LOCUS       DAHHYJ010000001        53373 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000001 DAHHYJ010000000
VERSION     DAHHYJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 53373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.001"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            342..1601
                     /locus_tag="NBK22_000001"
     CDS             342..1601
                     /locus_tag="NBK22_000001"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD2413225.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGA
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            1690..2025
                     /locus_tag="NBK22_000002"
     CDS             1690..2025
                     /locus_tag="NBK22_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2413226.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            2263..3690
                     /locus_tag="NBK22_000003"
     CDS             2263..3690
                     /locus_tag="NBK22_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCD2413227.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKDFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKDKETLYKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            3937..5253
                     /gene="rho"
                     /locus_tag="NBK22_000004"
     CDS             3937..5253
                     /gene="rho"
                     /locus_tag="NBK22_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCD2413228.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            5371..5625
                     /locus_tag="NBK22_000005"
     CDS             5371..5625
                     /locus_tag="NBK22_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCD2413229.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            5972..6571
                     /locus_tag="NBK22_000006"
     CDS             5972..6571
                     /locus_tag="NBK22_000006"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCD2413230.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            6572..7648
                     /gene="prfA"
                     /locus_tag="NBK22_000007"
     CDS             6572..7648
                     /gene="prfA"
                     /locus_tag="NBK22_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCD2413231.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            7635..8471
                     /gene="prmC"
                     /locus_tag="NBK22_000008"
     CDS             7635..8471
                     /gene="prmC"
                     /locus_tag="NBK22_000008"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCD2413232.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            8555..9601
                     /locus_tag="NBK22_000009"
     CDS             8555..9601
                     /locus_tag="NBK22_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCD2413233.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSI
                     AHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIIKGCEL"
     gene            9598..10017
                     /locus_tag="NBK22_000010"
     CDS             9598..10017
                     /locus_tag="NBK22_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCD2413234.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDLVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAHFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            10124..10648
                     /locus_tag="NBK22_000011"
     CDS             10124..10648
                     /locus_tag="NBK22_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCD2413235.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPDVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            10675..11913
                     /locus_tag="NBK22_000012"
     CDS             10675..11913
                     /locus_tag="NBK22_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCD2413236.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            11941..12570
                     /gene="upp"
                     /locus_tag="NBK22_000013"
     CDS             11941..12570
                     /gene="upp"
                     /locus_tag="NBK22_000013"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCD2413237.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            12594..13721
                     /gene="wecB"
                     /locus_tag="NBK22_000014"
     CDS             12594..13721
                     /gene="wecB"
                     /locus_tag="NBK22_000014"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCD2413238.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGQPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            13789..14241
                     /locus_tag="NBK22_000015"
     CDS             13789..14241
                     /locus_tag="NBK22_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCD2413239.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYFYLIIILGGLYLYTHHAFILGLIIGVTGSAINTCIFECYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYLNKAHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            14262..14990
                     /gene="atpB"
                     /locus_tag="NBK22_000016"
     CDS             14262..14990
                     /gene="atpB"
                     /locus_tag="NBK22_000016"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCD2413240.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            15033..15245
                     /gene="atpE"
                     /locus_tag="NBK22_000017"
     CDS             15033..15245
                     /gene="atpE"
                     /locus_tag="NBK22_000017"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCD2413241.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            15443..15964
                     /locus_tag="NBK22_000018"
     CDS             15443..15964
                     /locus_tag="NBK22_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCD2413242.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            15964..16503
                     /locus_tag="NBK22_000019"
     CDS             15964..16503
                     /locus_tag="NBK22_000019"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCD2413243.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINEELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            16525..18033
                     /locus_tag="NBK22_000020"
     CDS             16525..18033
                     /locus_tag="NBK22_000020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCD2413244.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            18064..18930
                     /locus_tag="NBK22_000021"
     CDS             18064..18930
                     /locus_tag="NBK22_000021"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCD2413245.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            18952..20364
                     /gene="atpD"
                     /locus_tag="NBK22_000022"
     CDS             18952..20364
                     /gene="atpD"
                     /locus_tag="NBK22_000022"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCD2413246.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            20384..20788
                     /locus_tag="NBK22_000023"
     CDS             20384..20788
                     /locus_tag="NBK22_000023"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCD2413247.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            21125..21217
                     /locus_tag="NBK22_000024"
     CDS             21125..21217
                     /locus_tag="NBK22_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413248.1"
                     /translation="MLKSNFPSPLSAVDVSFFESSYIFSYQDTL"
     gene            21349..21582
                     /locus_tag="NBK22_000025"
     CDS             21349..21582
                     /locus_tag="NBK22_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCD2413249.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            21693..22958
                     /gene="murA"
                     /locus_tag="NBK22_000026"
     CDS             21693..22958
                     /gene="murA"
                     /locus_tag="NBK22_000026"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD2413250.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            22992..23432
                     /gene="fabZ"
                     /locus_tag="NBK22_000027"
     CDS             22992..23432
                     /gene="fabZ"
                     /locus_tag="NBK22_000027"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCD2413251.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(23491..23931)
                     /locus_tag="NBK22_000028"
     CDS             complement(23491..23931)
                     /locus_tag="NBK22_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCD2413252.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDNEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            24125..24520
                     /locus_tag="NBK22_000029"
     CDS             24125..24520
                     /locus_tag="NBK22_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD2413253.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            24909..25604
                     /gene="sceD"
                     /locus_tag="NBK22_000030"
     CDS             24909..25604
                     /gene="sceD"
                     /locus_tag="NBK22_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCD2413254.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGVYNYTFDYEGFTYHFESDGTHFAWNYHATGANGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNAHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      25946..26048
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            26128..26817
                     /gene="tenA"
                     /locus_tag="NBK22_000031"
     CDS             26128..26817
                     /gene="tenA"
                     /locus_tag="NBK22_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCD2413255.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLLGNIQADALRHYLQAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYIKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDMELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            26810..27640
                     /gene="thiD"
                     /locus_tag="NBK22_000032"
     CDS             26810..27640
                     /gene="thiD"
                     /locus_tag="NBK22_000032"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD2413256.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRRLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            27624..28415
                     /gene="thiM"
                     /locus_tag="NBK22_000033"
     CDS             27624..28415
                     /gene="thiM"
                     /locus_tag="NBK22_000033"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCD2413257.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVAIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAFVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            28417..29058
                     /locus_tag="NBK22_000034"
     CDS             28417..29058
                     /locus_tag="NBK22_000034"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCD2413258.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKYDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            29144..30016
                     /gene="yidC"
                     /locus_tag="NBK22_000035"
     CDS             29144..30016
                     /gene="yidC"
                     /locus_tag="NBK22_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCD2413259.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(30787..31434)
                     /locus_tag="NBK22_000036"
     CDS             complement(30787..31434)
                     /locus_tag="NBK22_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD2413260.1"
                     /translation="MQQSDVISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKTFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     IVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERHDFMMMYLKQFFTEWNYHD"
     gene            complement(31462..32946)
                     /gene="cls"
                     /locus_tag="NBK22_000037"
     CDS             complement(31462..32946)
                     /gene="cls"
                     /locus_tag="NBK22_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD2413261.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKARYAKRSLWIKFKEGISQLLSPIL"
     gene            33112..33405
                     /gene="csoR"
                     /locus_tag="NBK22_000038"
     CDS             33112..33405
                     /gene="csoR"
                     /locus_tag="NBK22_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCD2413262.1"
                     /translation="MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            33417..33626
                     /locus_tag="NBK22_000039"
     CDS             33417..33626
                     /locus_tag="NBK22_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCD2413263.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            33754..33891
                     /locus_tag="NBK22_000040"
     CDS             33754..33891
                     /locus_tag="NBK22_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413264.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(34155..35357)
                     /locus_tag="NBK22_000041"
     CDS             complement(34155..35357)
                     /locus_tag="NBK22_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCD2413265.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPALIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKAIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            35675..36745
                     /locus_tag="NBK22_000042"
     CDS             35675..36745
                     /locus_tag="NBK22_000042"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCD2413266.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYPELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            36760..38118
                     /locus_tag="NBK22_000043"
     CDS             36760..38118
                     /locus_tag="NBK22_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCD2413267.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENAKCISIGVATDNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLGTLTGRRILILGDVLELGENSKEMHIGVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            38635..40155
                     /locus_tag="NBK22_000044"
     CDS             38635..40155
                     /locus_tag="NBK22_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD2413268.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(40508..41203)
                     /locus_tag="NBK22_000045"
     CDS             complement(40508..41203)
                     /locus_tag="NBK22_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2413269.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(41203..43860)
                     /locus_tag="NBK22_000046"
     CDS             complement(41203..43860)
                     /locus_tag="NBK22_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCD2413270.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKITTNFTKHGSHHFQYLDVDRIIEESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYAHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDTLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSFIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITIYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVTLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVNLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SYEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIYTDFNEGDV"
     gene            43998..44108
                     /gene="kdpF"
                     /locus_tag="NBK22_000047"
     CDS             43998..44108
                     /gene="kdpF"
                     /locus_tag="NBK22_000047"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCD2413271.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            44132..45808
                     /gene="kdpA"
                     /locus_tag="NBK22_000048"
     CDS             44132..45808
                     /gene="kdpA"
                     /locus_tag="NBK22_000048"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCD2413272.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIG
                     NFYQDIVRFIVRVVLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            45827..47854
                     /gene="kdpB"
                     /locus_tag="NBK22_000049"
     CDS             45827..47854
                     /gene="kdpB"
                     /locus_tag="NBK22_000049"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCD2413273.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPVDGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYIYELPKDIDGTYKTFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            47874..48434
                     /locus_tag="NBK22_000050"
     CDS             47874..48434
                     /locus_tag="NBK22_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCD2413274.1"
                     /translation="MNTIRNSICLTIITMVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTETRYFHGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNTELIA
                     RMKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADARHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDKMKD"
     gene            48643..49122
                     /locus_tag="NBK22_000051"
     CDS             48643..49122
                     /locus_tag="NBK22_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413275.1"
                     /translation="MSDYTKLSKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLCIKPLWQYHCRFYKVDQLSVQYRTSFLIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTIYLPMMSHDDVKIIEARTMSNLRGVESDV"
     gene            49115..50698
                     /locus_tag="NBK22_000052"
     CDS             49115..50698
                     /locus_tag="NBK22_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD2413276.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEVAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTKRLQAVVERQSYIRRLFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVPILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPLSGFHRH
                     FLIPSIIVIAMAGIGCYFWNQLLLLFTIITVVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKARHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            50685..51176
                     /locus_tag="NBK22_000053"
     CDS             50685..51176
                     /locus_tag="NBK22_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD2413277.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVLFWIPIALVLLTAFNILNWLFWEI
                     LDNYISIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLYTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            51180..51539
                     /locus_tag="NBK22_000054"
     CDS             51180..51539
                     /locus_tag="NBK22_000054"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCD2413278.1"
                     /translation="MIHGIGVDLIEIDRIQLLYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            51605..52753
                     /gene="alr"
                     /locus_tag="NBK22_000055"
     CDS             51605..52753
                     /gene="alr"
                     /locus_tag="NBK22_000055"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD2413279.1"
                     /translation="MSNKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDN
                     HRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            52838..53008
                     /gene="mazE"
                     /locus_tag="NBK22_000056"
     CDS             52838..53008
                     /gene="mazE"
                     /locus_tag="NBK22_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCD2413280.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NETYLSSNSTNE"
     gene            53005..53367
                     /locus_tag="NBK22_000057"
     CDS             53005..53367
                     /locus_tag="NBK22_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD2413281.1"
                     /translation="MIRRGDVYLADLSPVKGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
BASE COUNT        18761 a         7883 c        10146 g        16583 t
ORIGIN      
        1 gaaagcgtaa aaatggtaaa atataccaaa attgattgtg atataattat aaggaaaatg
       61 agcaatttat gaaaaaagtt tacgtacaaa tcgtagaatt aaaactaaat aattatcaaa
      121 acaacgtcaa tatttagttg aataatcaga ctttagacca tggtcaagtg gggaagaaca
      181 gcatatatta gtaaaggtga atgatttgtt attaatcaat cgaaaataga aagacaagat
      241 tttaacgatt aaaataaact attttacaaa taaagtaaaa ttaatttata atgctaataa
      301 tgcaaaaaat taaaaagtaa tggacaaagg agataaatga tatggctcaa gaggtaataa
      361 aaataagagg tggacgcaca ctaaatgggg aagttaatat cagtggcgcg aaaaacagtg
      421 ctgtagctat cattcctgca acattattag ctcaaggaca tgtgaaatta gaagggttac
      481 cgcaaatctc tgatgttaaa actttagtga gtttattaga agatttaaat attaaggcat
      541 cactaaatgg tacggaatta gaagttgata caactgaaat acaaaatgct gcattaccaa
      601 ataataaagt tgaatcatta cgtgcttcat actatatgat gggtgccatg ttaggtagat
      661 ttaaaaaatg tgtgattggt ttaccgggag gatgtccact tgggccacgt ccaattgatc
      721 aacatattaa aggatttaaa gctttaggtg ctgaaattga tgaatcaagc acgacatcaa
      781 tgaaaattga agctaaagaa ttaaaaggtg cgcatatttt tctagatatg gttagcgtag
      841 gtgcaacaat taacattatg ctagctgcag tttatgcaac aggacaaact gtaattgaaa
      901 atgctgctaa agaaccggaa gtagttgatg ttgctaactt cttaacaagt atgggagcta
      961 atattaaggg agcgggtaca tcaacaatta aaatcaatgg tgtcaaagaa ttacatggtt
     1021 ctgagtatca agttatacct gatagaattg aagcaggcac atatatgtgt atcgctgcag
     1081 catgtggaga aaatgttata ttaaataata ttgttccgaa acatgtagaa acattaactg
     1141 ctaaatttag tgaattaggt gtgaatgttg atgtaagaga cgaaagaatt cgcatcaata
     1201 ataatgcgcc atatcaattt gttgatatta aaacactagt atatccaggt tttgctactg
     1261 atttgcaaca gcctattaca ccattattat ttatggcaaa tggtccttca tttgtaactg
     1321 atacgattta tccggagcgt tttaaacatg ttgaagaatt aaagcgcatg ggtgcaaata
     1381 ttgaagttga cgaaggcaca gcaacgataa aaccatcaac attacatggt gctgaagttt
     1441 atgctagcga tttaagagca ggagcttgtt taattattgc tggtttaatt gctgagggtg
     1501 taactacaat ttacaatgta aaacatattt atagaggtta tacagatatt gtagaacact
     1561 taaaagcttt aggtgcagat atttggacgg aaactgtata gatttatctt gttttgtggt
     1621 ataataatta tactgattaa aaaagggtgt tcgtttacat tctaaattta ttgatgagag
     1681 gtgaagatga tggaagtatg tccgtatctc gaagaaactt ttaaaatact tggtagaagt
     1741 tggaatggat taatcattaa ttatctctca agatgtaatg actgttcagc acacttttcc
     1801 gatatgaaaa gagatttgaa aacaataaca ccgcgtgctt taagtcttaa gttgtcagag
     1861 cttgcacaat gggagttagt tgagaagcaa atcatttcta ccagtccagt acaaattatt
     1921 tatgtgctaa ctgaaaaagg taaagcgtta gcagaggctt tacatccaat tgaagcatgg
     1981 gcgcaatcat atgtcgattt aacagatcaa cgtactgcta aataattaca tgacgtttaa
     2041 acatagcatt gattataact atttctaagt cttcgcatta tttgcgatga tgtgggaata
     2101 gttattttta tttaaaaata taaaaaatta gatgcagcaa aattttaaag cattttattt
     2161 tgaacatatt aaaagggagc gtatcataat ggaatgtaat gtttatatcg tatgcattac
     2221 ggataaataa tatataaatc attcttgagg agtgaaagaa taatgagaga ctacacaaag
     2281 caatacatta atggcgaatg ggtagaaagt aatagtaatg aaacgataga agttataaat
     2341 ccagcaaccg aagaagtaat cgggaaagtt gctaaaggta ataaagctga tgttgataaa
     2401 gccgtcgagg cggcagacga tgtttattta gagttccgtc atacatctgt gaaagaaaga
     2461 caagcgttat tagataaaat tgtaaaagaa tatgaaaaca gaaaagacga tattgtacaa
     2521 gctattacgg atgaattagg tgctccttta tcattatctg agcgtgtcca ttatcaaatg
     2581 ggactaaacc attttgttgc agcgagagac gcattagata actacgaatt tgaagaacgc
     2641 cgcggagatg atttagttgt taaagaagca atcggtgtat ctggattaat tacaccgtgg
     2701 aacttcccta caaaccaaac atcattaaaa ttagcagcag catttgcggc tggtagtcca
     2761 gttgtactta aaccatctga agaaacacca tttgcagctg ttattttagc tgagattttt
     2821 gataaagtcg gtgttcctaa aggtgtattt aaccttgtta atggtgatgg tgctggtgtt
     2881 gggaatcctt tatctgaaca tcctaaagta cgcatgatgt catttacagg atcaggccct
     2941 actggttcta aaattatgga aaaagccgct aaagatttta aaaaggtatc attagagctt
     3001 ggtggcaaat caccatacat cgtcctagat gacgtagata ttaaagaagc ggctaaagca
     3061 acaacaggca aagttgttaa taatactggt caagtatgta cagctggtac acgtgtttta
     3121 gtgcctaaca aaattaaaga tgcattctta gctgaattaa aagaacaatt tagccaagtg
     3181 cgtgtcggta atccaagaga agatggtaca caagtaggcc ctatcattag taaaaaacaa
     3241 tttgatcaag tacaaaatta tattaataaa ggtattgaag aaggtgctga attattttat
     3301 ggtggtcctg gtaaaccaga aggacttgaa aaaggatact ttgcacgtcc gacaattttt
     3361 attaatgtag ataatcaaat gacgatagca caagaagaaa tttttgggcc agtaatgtca
     3421 gttatcactt ataacgattt agatgaagcg attcaaattg caaatgatac aaaatatggt
     3481 ttggcaggat atgttattgg taaagacaaa gaaacattgt ataaagtagc tcgttctatt
     3541 gaagcaggta cagtagaaat aaacgaagca ggcagaaagc cagacttacc atttggtggc
     3601 tataaacaat ctggtttagg tcgtgaatgg ggcgattatg gtattgaaga gttcttagaa
     3661 gtgaaatcaa tagctggcta ttttaaataa atttgatttt tgaattaaaa atcgcaataa
     3721 aacagtgcac atgactaatt aagttttgtg tactgtttta attttgcaat ttttataaat
     3781 agattttgta attaaaataa aaatttgcta tagttattca tgtatttaaa aggttgggga
     3841 ttagcataat gggattgtgc tagcacagtt atttatgcat tgtcatgcct atctattact
     3901 tactaactaa aaaataatga aatgggtgta aactatatgc ctgaaagaga acgtacatct
     3961 cctcagtatg aatcattcca cgaattgtac aagaactata ctaccaagga actcactcaa
     4021 aaagctaaaa ctcttaagtt gacgaactat agtaaattaa ataaaaaaga acttgttcta
     4081 gctattatgg aagcacaaat ggaaaaagat ggtaactatt atatggaagg tatcttagat
     4141 gatatacaac cagatggtta tggtttttta agaacagtga actattctaa aggggaaaaa
     4201 gatatttata tatctgctag ccaaattcgt cgttttgaaa ttaaacgtgg ggataaagta
     4261 actgggaaag ttagaaaacc taaagataac gaaaaatatt atggcttatt acaagttgac
     4321 tttgtcaatg accataacgc agaagaagtg aagaaacgac cgcatttcca agctttgaca
     4381 ccactttatc cagatgagcg tattaaatta gagacagaaa cacaaaatta ttcaacgcgt
     4441 atcatggatt tagtaacacc gattggttta ggtcaacgtg gtttaatagt ggcgccacct
     4501 aaagcaggta aaacatcgtt attaaaagaa atagcgaatg caatcagtac gaacaaacca
     4561 gatgcaaagc tatttatttt gttagttggc gagcgtcctg aagaggtaac agatttagaa
     4621 cgctcagtag aagctgctga agtcgttcat tcaacgtttg acgaaccacc agaacaccat
     4681 gttaaagtag ctgaattatt acttgaacgt gcaaagcgtt tagtagaaat tggggaagat
     4741 gtcattattt taatggattc tataacgaga ttagcacgcg cttataactt agttattcca
     4801 ccaagtggtc gtacattatc aggtggttta gatcctgcat ctttacacaa accaaaagca
     4861 ttcttcggtg cagcgagaaa tattgaagcg ggtggaagtt taacaatact tgcaactgca
     4921 ttagttgata cgggttcacg tatggacgat atgatttacg aagaatttaa aggaacaggt
     4981 aacatggagt tacatttaga tcgtaaattg tctgaacgtc gtatcttccc tgcaattgat
     5041 attggcagaa gttcaacgcg taaagaagaa ttgttgataa gtaaatctga attagacaca
     5101 ttatggcaat taagaaatct attcactgac tcaactgact ttactgaaag atttattcgc
     5161 aaacttaaaa ggtctaagaa taatgaagat ttcttcaagc agctacaaaa gtctgcagaa
     5221 gaaagtacta aaacgggtcg acctataatt taataaacat tatatagggg cttgcgtttt
     5281 gaattaatta cctttataat tacacagtat tgggtaaaaa ctcacaaata actctgttcc
     5341 agatggttca gggcaaagga gctgaaaata atgaaacaag gaattcatcc tgagtatcac
     5401 caagtaatct ttttagatac tactacaaac ttcaaattct taagcggttc tacaaaaaca
     5461 tcttcagaaa tgatggagtg ggaagatgga aaagaatacc cagttattcg tttagatatt
     5521 tcatctgatt cacatccatt ctacactgga cgtcaaaaat tcgctgctgc ggatggacgt
     5581 gtggaacgtt tcaacaaaaa gtttggtctt aaatcaaaca actaatatag taatcgttaa
     5641 gctgtctgca tcctataatg gaattgtaga cagctttttt atttataagc gtagtaaggg
     5701 tatatactta cgactgtgtt caaagaagtg actcaatacc atgttgttaa tacataatga
     5761 taaatttccc cctttattaa agtttgtatt gagtagaaac accgcacgaa taagaagcaa
     5821 caaattagaa tagctgtata taaatatgca agacaaattt ataaaataat tgttcatgta
     5881 caatatcgat ataattatga aaaagacatt tatgaatatt tgaaatatga tataataaag
     5941 cgattatgtt ttgaaaaagg tggaactctc aatgtatgaa acttaccatt ccggttggat
     6001 agaatgtatt acaggtagta tgtttagcgg taaatccgaa gaactaataa gacgtctaag
     6061 aaggggtata tacgctaaac aaaaagtcgt tgtatttaaa ccggcgattg atgatcgata
     6121 tcataaagaa aaagtggtct ctcataatgg taatgccatt gaagcgatta atatttctaa
     6181 agcaagtgaa attatgacgc acgacttaac taatgttgat gttataggaa tagacgaagt
     6241 gcaatttttt gacgatgaaa ttgtaagtat agtagaaaaa ctatcagcag atggacaccg
     6301 tgttattgtt gctggattag atatggattt taggggcgaa cccttcgaac caatgcctaa
     6361 attgatggct gtcagtgaac aagtaacgaa acttcaagca gtttgtgctg tttgtggttc
     6421 ttcatctagt agaacgcaac gattaataaa tggtaaacca gctaaaatcg atgatccgat
     6481 tatcttagta ggtgccaatg aaagttatga gccacgttgt cgcgcgcacc atatcgtagc
     6541 acctagtgat aataataagg aggaattata agtgtttgat caattagata ttgtagaaga
     6601 aagatacgaa cagttaaatg aactgttaag tgacccagat gttgtaaatg attcagataa
     6661 attacgtaaa tattctaaag agcaagctga tttacaaaaa actgtagatg tttatcgtaa
     6721 ctataaagct aaaaaagaag aattagctga tattgaagaa atgttaagtg agactgatga
     6781 taaagaagaa gtagaaatgt taaaagagga gagtaatggt attaaagctg aacttccaaa
     6841 tcttgaagaa gagcttaaaa tattattgat tcctaaagat cctaatgatg acaaagacgt
     6901 tattgtagaa ataagagcag cagcaggtgg tgatgaggct gcgatttttg ctggtgattt
     6961 aatgcgtatg tattcaaagt atgctgaatc acaaggattc aaaactgaaa tagtagaagc
     7021 gtctgaaagt gaccatggtg gttacaaaga aattagtttc tcagtttctg gtaatggcgc
     7081 gtatagtaaa ttgaaatttg aaaatggtgc gcaccgcgtt caacgtgtgc ctgaaacaga
     7141 atcaggtgga cgtattcata cttcaacagc tacagtggca gttttaccag aagttgaaga
     7201 tgtagaaatt gaaattagaa atgaagattt aaaaatcgac acgtatcgtt caagtggtgc
     7261 aggtggtcag cacgtaaaca caactgactc tgcagtacgt attacccatt taccaactgg
     7321 tgtcattgca acatcttctg agaagtctca aattcaaaac cgtgaaaaag caatgaaagt
     7381 gttaaaagca cgtttatacg atatgaaagt tcaagaagaa caacaaaagt atgcgtcaca
     7441 acgtaaatca gcagtcggta ctggtgatcg ttcagaacgt attcgaactt ataattatcc
     7501 acaaagccgt gtaacagacc atcgtatagg tctaacgctt caaaaattag ggcaaattat
     7561 ggaaggccat ttagaagaaa ttatagatgc actgacttta tcagagcaga cagataaatt
     7621 gaaagaactt aataatggtg aattataaag aaaagttaga tgaagcaatt catttaacac
     7681 aacaaaaagg gtttgaacaa acacgagctg aatggttaat gttagatgta tttcaatgga
     7741 cgcgtacgga ttttgtagtc cacatgcatg atgatatgcc gaaagcgatg attatgaagt
     7801 tcgacttagc attacaacgt atgttattag gagagcctat acagtatata gttggctttg
     7861 cctcatttta tggtagaacg tttgatgtaa actcaaattg tttgatacca agacctgaaa
     7921 ccgaagaagt aatgttgcat ttcttacaac agttagaaga tgatgcaaca atcgtagata
     7981 tcggaacggg tagtggtgta cttgcaatta ctttgaaatg tgaaaagccg gatttaaatg
     8041 ttattgctac tgatatttca cttgaagcaa tgaatatggc tcgtaataat gctgagaagc
     8101 atcaatcaca aatacaattt ttaacagggg atgcattaaa gcccttaatt aatgaaggta
     8161 tcaagttgaa cggcttgata tctaatccac catatataga tgaaaaagat atggttacga
     8221 tgtctccaac ggttacgaga ttcgaaccac atcaggcatt gtttgcagat aaccatggat
     8281 atgctattta tgaatcaatc attgaagatt tacctcacgt tatggaaaaa ggcagcccag
     8341 ttgtttttga aattggttac aatcaaggtg aggcacttaa atcaataatt ttaaataaat
     8401 ttcctgacaa aaaaatcgac attattaaag atataaatgg ccacgatcga atcgtctcat
     8461 ttaaatggta attagaagtt atgcctttgc tatgattagt taagtgcata gctttttgct
     8521 ttatattatg ataaataaga aaggcgtgat taagttggat actaaaattt gggatgttag
     8581 agaatataat gaagatttac agcaatatcc taaaattaat gaaataaaag acattgtttt
     8641 aaacggtggt ttaataggtt taccaactga aacagtttat ggacttgcag caaatgcgac
     8701 agatgaagaa gctgtagcta aaatatatga agctaaaggc cgtccatctg acaatccgct
     8761 tattgttcat atacacagta aaggtcaatt aaaagatttt acatatactt tggatccacg
     8821 cgtagaaaag ttaatgcagg cattctggcc gggccctatt tcgtttatat tgccgttaaa
     8881 gccaggctat ctatgtcgaa aagtttctgg aggtttatca tcagttgctg ttagaatgcc
     8941 aagccattct gtaggtagac aattattaca aatcataaat gaacctctag ctgctccaag
     9001 tgctaattta agtggtagac cttcaccaac aactttcaat catgtatatc aagatttgaa
     9061 tggccgtatc gatggtattg ttcaagctga acaaagtgaa gaaggattag aaagtacggt
     9121 tttagattgc acatcttttc cttataaaat tgcaagacct ggttctataa cagcagcaat
     9181 gattacagaa atacttccga atagtatcgc ccatgctgat tataatgata ctgaacagcc
     9241 aattgcacca ggtatgaagt ataagcatta ctcacccaat acaccactta caattattac
     9301 agatattgag agcaaaattg gaaatgacgg taaagattgg tcttctatag cttttattgt
     9361 gccgagtaat aaggtggcgt ttataccaag tgaagcgcaa ttcattcaat tatgtcagga
     9421 tgacaatgat gttaaacaag caagtcataa tctttatgat gtgttacatt cacttgatga
     9481 aaatgaaaat atttcagcgg cgtatatata cggctttgag ctgaatgata atacagaagc
     9541 aattatgaat cgcatgttaa aagctgcagg taatcacatt attaaaggat gtgaactatg
     9601 aagattttat tcgtttgtac aggtaacaca tgtcgtagcc cattagcgga aagtattgca
     9661 aaagaggtta tgccaaatca tcaatttgaa tcaagaggta tattcgctgt gaacaatcaa
     9721 ggtgtttcga attatgttga agacttagtt gaagaacatc atttagctga aacgacctta
     9781 tcgcaacaat ttactgaagc agatttgaaa gcagatatta ttttgacgat gtcgtattcg
     9841 cacaaagaat taatagaggc acactttggt ttgcaaaatc atgttttcac attgcatgaa
     9901 tatgtaaaag aagcaggaga agttatagat ccatacggcg gaacaaaaga aatgtatgta
     9961 catacctatg aagaacttgt aagtttaatt ttaaaattaa aagatattat ttgctagtag
    10021 gaattctgta taattagtat atgttaacag ttaagtaagt tataatagag gagtgtagca
    10081 ctttgctata ttcctttttt atttttagga agggtgggct tttatgaaag atttgacaat
    10141 gttattagac gaattaaaag atatgtcctt ttttaataaa ggagatatat gtttaattgg
    10201 atgttcaaca tcggaagtca ttggcgaaaa aatagggact gtcggttcga tggaagttgc
    10261 agaaactatt tttaatgcat tagatgttgt gagtaaagag acaggcgtta cttttgcttt
    10321 tcaaggatgt gaacatatca acagagcgat aacaatagaa aaatctcagt ataatccatt
    10381 aacaatggaa gaagtttccg tcgttccaga tgtacatgct ggagggagtt tggcaacata
    10441 cgcttttcag catatgaagg atccgatcgt ggtcgaacat attactgttc cttgtggaat
    10501 tgatattggt caaacattaa taggcatgca tattaaacat gtatgtgtac ctgtaaggac
    10561 ttcagtaaag caagtaggac aggcaattgt aactattgcc acatctaggc cgaaaaagat
    10621 tggtggcgaa cgtgctaaat accaataagt ttttttgaaa gaaggggaga ttttatgtct
    10681 tatatcacca agcaagataa agttatcgca gaagcaatcg agagagaatt tcagagacaa
    10741 aatagcaaca tagagttaat cgcatcggaa aattttgtat cggaagcggt tatggaagca
    10801 caaggttcag tgttgactaa taagtatgct gaaggctatc caggacgccg atattatggt
    10861 ggatgtgagt ttgtagatgt tactgaaagc atcgcaattg atcgtgctaa agcattgttt
    10921 ggagctgaac atgtcaatgt tcaaccacat tcaggttcac aagcgaacat ggctgtttac
    10981 ttagttgcat tagaaatggg cgacacagtt ttaggtatga atttgagtca tggtggtcac
    11041 ttgacacatg gagcgcctgt taattttagt ggtaaattct acaatttcgt tgaatatgga
    11101 gtagataaag acacagaacg aatcaattat gatgaagttc gtaaattagc gttagagcat
    11161 aagcctaagc ttattgtggc aggagcatca gcatattcaa gaacaattga cttcaaaaag
    11221 tttaaagaaa tcgcagatga agtaaacgct aagttaatgg tagacatggc acatattgca
    11281 ggattagtag cggcaggttt acatccaaat ccagtagaat atgctgattt tgtaacaact
    11341 acaacacaca aaacattacg cggaccacgt ggtggtatga ttttatgtaa ggaagaatat
    11401 aaaaaagaca tagataaaac aattttccct ggtattcaag gtggacctct tgagcatgtt
    11461 attgcagcaa aagcagttgc ttttggagaa gcgttagaaa ataatttcaa aacgtatcaa
    11521 caacaagtgg ttaaaaacgc aaaagttctt gcagaagcat taattaatga aggatttaga
    11581 attgtttctg gcggtacaga taatcactta gtagctgttg atgtaaaagg gtctatagga
    11641 cttactggta aagaagctga agagacttta gattcagttg gtatcacatg taacaaaaat
    11701 accattccgt tcgatcaaga aaaacctttt gtaacgagtg gtatacgttt aggtacacct
    11761 gctgcaacaa cgcgtggatt tgatgaaaaa gcttttgagg aagttgcaaa aatcatcagt
    11821 ttagcattga aaaatagtaa agatgaagaa aaattacaac aagctaaaga acgcgttgcg
    11881 aaattaacag ctgaatatcc tctatatcaa taaacataca caaagatagg agtaattttt
    11941 atgagtaaag tacacgtttt cgatcatcca ttaattcaac acaaactaag ctatattaga
    12001 gatgtaaata caggtactaa agaatttaga gaacttgtag acgaagttgg tatgttgatg
    12061 gcatatgaag taactagaga cttagagctt caggatgttg atattgaaac gcctgtaact
    12121 aaaatgacgg ctaaacgatt agctggtaaa aaattagcta tcgttccaat tttaagagct
    12181 ggtttaggta tgactgatgg tattttaagt ttagtacctg cagccagagt tggacatatc
    12241 ggtttatatc gagatcccga aacattaaag gctgtagagt attttgctaa gttacctcaa
    12301 gatattactg aaagacaaat cattgttgtt gatcctatgt tagcaacagg cgcatcagca
    12361 attgaagcta ttacttcatt gaagaaacgt ggcgctaaaa atattcgctt catgtgttta
    12421 attgcagcac cagaaggcgt agaaaagatg catgaagcac atccggatgt agatatttat
    12481 attgctgcac ttgatgaaaa gctaaatgat aaagcatata tcacaccagg gttaggcgac
    12541 gctggagata gattgtttgg tactaaatag aagaatcgga gataagtagt ttgatgaaaa
    12601 agattatgac catatttgga acaagacccg aagccataaa gatggctcct ttagttaaag
    12661 ctttggaaca agaaaaaatg ctcgagccaa ttgttgttgt tacagcacag catagagaaa
    12721 tgcttgattc agtattaagt acctttgaaa tcaaaccaaa atacgatttg aatattatga
    12781 aatcaggaca gacactttct gagataactt ctaaatcaat cactcaatta gaacaagtga
    12841 ttcaattaga aaaaccagac atggtacttg tacatggtga tacgatgacg acatttgcag
    12901 gtggtttagc ggcattttat aaccaagtgc cgattggaca cgttgaagca gggcttagaa
    12961 gttatgataa atattcacct tttccggaag aggtgaatag acaattagtt ggtgtattag
    13021 ctgacttaca ttttgcacct actaaaaatg ctgcatcgca tttgttaagt gaagggaaat
    13081 attcagaaag tgttgttgtc actggtaata cagcgattga tgctatgaaa tatacagttg
    13141 atgacaatta taaatctaac atcatggata aataccatga caaaaaattc attcttatga
    13201 cagcacaccg acgagaaaat atcgggcaac ccatggaaaa tatatttaaa gcggttagac
    13261 gcttaataga tgagtatact gatttagcac tagtatatcc aatgcataaa aacccgaaag
    13321 ttcgagaagt ggctcaaaag attttaggta gtcatgacag aattgaattg attgaacctt
    13381 tggacgtagt agatttccat aattttgcta aaaaatctta ttttattttg acagattcag
    13441 gtggaattca ggaagaagcg ccatcattta ataagccagt tttagttttg cgaagcgtta
    13501 cagaacgtcc tgaaggtgtt gaagctggca cattaaaagt cattggaaca aataagcaaa
    13561 atgtgtatca agctgcaaaa gaattaatcg atgatgagag attatatcat caaatgtctg
    13621 aagcatctaa tccttacggt gatggatttg cttcagaaag aatagttaat catattaagt
    13681 attatttgaa tttaatcact gaaaaaccaa gcgatttctg aggaaaatag actttttgtc
    13741 taattgtgtc taaattgtgt ctaactatgc ttattttttt acaacaattt gaatagaatt
    13801 gacaccacaa atcgcctata ttatcattta gtttgtatga gtggaacggt tttcatgatt
    13861 gatatcagtc acacggaggc atataaaatg agtcgtttct acaacatttt taaacagttc
    13921 attcaatatt atttttatct aataataata ttgggaggat tataccttta tacacaccat
    13981 gcatttatct taggattaat cattggtgtt actggttctg caatcaacac atgtattttt
    14041 gaatgctatt tagctaaagc taaaagacca gacactatgc atatttcaac tggaaatatg
    14101 tggcgatact tagttgcaat tattgcctgt atgatttggt accttaataa agcgcatgta
    14161 agtatcatcg gtataattat tggtttaatg atttcatatg ttgtagttat catacgtcct
    14221 ttactaaagg tgagcaaata aattaagaaa gaggtgagat tatggatcac aaatccccgc
    14281 tcgtgagttg gaatttattc ggttttgata tcgttttcaa tttatcaagt atattgatga
    14341 tacttgttac ggcgtttctt gtttttctac ttgctatcat ttgtacgcgt aatttgaaaa
    14401 aaagaccaac tggcaaacaa aatttcgttg aatggatttt tgatttcgtg aggggaatca
    14461 ttgaaggtaa catggcttgg aaaaaaggtg gtcaattcca cttcttagca gtaacgctga
    14521 ttctgtacat ttttatagct aatatgttag gtcttccgtt ttctatagta acgaaagatc
    14581 acacattgtg gtggaaatca ccgacagctg atgcaacagt gactttaacg ttgtctacaa
    14641 cgataatact gttaactcac ttttatggaa ttaaaatgcg tggtacgaaa caatatctta
    14701 aaggttatgt acagccgttt tggccattgg caattattaa tgtttttgaa gagttcactt
    14761 caacattaac gcttggtctg cgtttgtacg gtaacatatt tgcaggtgag atactattaa
    14821 cattacttgc tggcttattc tttaacgaac cagcatgggg ttggattatt agtatcccag
    14881 gattaattgt ttggcaagca ttttcaatat ttgtaggaac aatccaagca tatatcttta
    14941 ttatgctttc gatggtttat atgtcacata aagtggcaga tgaacactaa aaatttcaat
    15001 aattatatac aatcacagga ggaaattaaa ttatgaattt aatcgcagca gcaatcgcaa
    15061 ttggtttatc agcattagga gcaggtatcg gtaacggttt aatcgtttca agaacagttg
    15121 aaggtgtagc acgtcaacca gaagcacgtg gtcaattaat gggtatcatg ttcattggtg
    15181 taggtttagt tgaggcatta cctatcatcg gtgtagtaat tgcattcatg acatttgctg
    15241 gataattaac agataaaaga ggtcgggaca aagcgcatag gacataattc atgatgcata
    15301 tatagtaata tctttgaact ttattaaata gttgagatat gaatgcacca tgcctatcgc
    15361 ataaattcag taggtcctaa cctcgtcgtt tttttctata taacactagc gattatttta
    15421 atgaaaggag tgtcatgaac ccgtgactga aacagctaac ttattcgttc ttggtgcagc
    15481 tggaggcgtt gagtggggta ctgtgattgt acaggtccta actttcatcg tgttacttgc
    15541 gttacttaaa aagttcgcat ggggtccatt gaaagatgta atggataaac gtgaaagaga
    15601 tattaacaga gatatcgatg acgcagaaca agctaagtta aatgcacaga aacttgaaga
    15661 agaaaataaa caaaaactta aagaaacaca agaagaagtt caaaagattt tagaagatgc
    15721 taaggttcaa gcacgtcaac agcaagaaca aattattcat gaagcaaacg tacgtgcaaa
    15781 cggtatgatt gaaacagcac aaagtgaaat caatagccaa aaagaacgtg ccattgcaga
    15841 tattaataat caagtatctg aactatcagt gttaattgct tctaaagttc ttagaaaaga
    15901 aatttctgaa caagaccaaa aagcattggt tgacaagtat ctaaaagagg caggcgataa
    15961 ataatggtaa aagtagctaa caagtatgct aaagcattat ttgacgtgtc attagataca
    16021 aataatttag agactattaa tgaagaatta acagttataa atgaagcagt aaaagataaa
    16081 attgagcaat tgaaaatggt tgacagtaat ccaactcaaa cagcagaaca acgtcgtgag
    16141 ttgattaatg gtgtatttac tgacattaat ccatacatta aaaatatgat gtacgtgtta
    16201 gcagataata gacatatctc attaatagct gacgtattca aggcgttcca aagcttatat
    16261 aacggacact acaatcaaga ttttgcaaca attgagtcaa catatgaatt gagtcaagaa
    16321 gagttagata agattgtcaa actagtaact caacaaacga agttatctaa agttattgta
    16381 gatacaaaaa ttaatccaga tttaattggt ggatttagag ttaaagtcgg cacaactgta
    16441 ttagatggta gtgttagaaa tgatcttgtc caattacaaa gaaaatttag aagagttaat
    16501 taattataaa gaggagtgac atagatggcc ataaaagctg aagaaatcag tgcattactt
    16561 cgctcacaaa ttgaaaatta tgagtcagaa atgtccgtaa ctgatgtagg tactgtatta
    16621 caaattggtg atggtattgc attaattcac ggattaaatg acgttatggc tggtgagcta
    16681 gtagaattcc ataacggcgt acttggttta gcccaaaacc ttgaagagtc aaacgtgggt
    16741 gtggttattt taggaccata cacaggtatt actgaaggtg acgaagttaa acgtactggt
    16801 cgtatcatgg aagtaccagt aggtgaagaa ctaatcggaa gagttgttaa tccattagga
    16861 caacctattg atggacaagg accgattaac acaactaaaa cacgtccagt agagaaaaaa
    16921 gctactggtg taatggatcg taaatcagta gatgagccat tacaaacagg tatcaaagca
    16981 attgatgctt tagtaccaat tggtagaggt caacgtgagt taatcatcgg tgaccgtcaa
    17041 acaggtaaaa caacaattgc aattgacaca attttgaacc aaaaagatca aggtacgatt
    17101 tgtatctatg ttgctattgg tcaaaaagat tcaacagtaa gagcaaatgt tgaaaagtta
    17161 agacaagcag gcgctttaga ctacactatt gttgtagcag catcagcttc tgaaccttct
    17221 ccattattat atattgcacc atattcaggt gtaacaatgg gtgaagaatt catgtttaac
    17281 ggtaaacatg ttttaatcgt ttatgatgat ttaactaaac aagcagcagc ttatcgtgag
    17341 ttatcattat tattacgtag acctccaggt cgtgaagcat acccaggtga cgtattctac
    17401 ttacatagta gattattaga aagagcagca aaattaaacg atgacttagg tggcggttca
    17461 attactgcat taccaattat cgaaacacaa gctggtgata tttcagctta tgtaccaaca
    17521 aacgttattt caattacaga tggacaaatc ttcttacaat ctgatttatt cttctcaggt
    17581 gtaagaccag cgattaatgc cggacaatct gtatctcgtg ttggtggatc tgcacaaatt
    17641 aaagcaatga agaaagttgc tggtacgtta cgtcttgact tagcgtcata cagagaactt
    17701 gaatcatttg cacaattcgg ttcagacctt gatgaattta ctgcaagtaa attagaacgt
    17761 ggtaaacgta ctgttgaagt cttaaaacaa gatcaaaaca aaccattacc agtcgaacac
    17821 caagtgttga ttatttatgc attaacaaaa ggatatttag atgatattcc agttgtagat
    17881 atcacacgtt ttgaagacga gttaaaccac tgggcagaat caaatgctac tgaactgtta
    17941 aatgaaatca gagaaactgg tggcttacca gatgctgaga agtttgacac agcaattaac
    18001 gaattcaaaa aaagctttag caaatctgaa taataaacaa gtttagtata aggtggtgag
    18061 atagtggctt ctcttaaaga aatagatact cgaataaaat caaccaaaaa aatgaagcag
    18121 attacgaaag cgatgaacat ggtatcaagt tcaaaacttc gtagagctga aaaaaataca
    18181 aaacaattca caccatatat ggataaaatg caagatgcaa ttactgcagt tgcaggggca
    18241 agtagcaata caaaccatcc aatgctaaga cctcgtaaaa ttactagaag tggatattta
    18301 gttatcacga gtgataaagg tttagcaggt gcatatagtg caaacgtgct taaaaaattg
    18361 attactgata ttgaagcgaa acatcaagat agtagcgaat acagtattgt agttttaggg
    18421 caacaaggtg ttgatttcct aaaaaataga ggttatgaca ttgagtattc tcaagtagac
    18481 gtacctgatc aaccttcttt caaatctgtt caagcactag ctaaccatgc tatagactta
    18541 tacagtgaag aagaaattga tgaattaaat atatactata gtcattatgt cagcgttctt
    18601 gaaaacaagc ctacatctag acaagtatta ccattatctc aagaggattc tagtaagggg
    18661 catggtcatt tgtcttctta tgaatttgag ccagataaag aatctatctt aagtgtaatc
    18721 ttgcctcaat atgttgagag tttgatttac ggaacaatat tagacgcaaa agcaagtgag
    18781 catgcaacac gtatgactgc gatgaaaaat gccactgata atgcaactga acttattgat
    18841 gacttatcat tagaatataa cagagcgaga caagcagaaa ttacgcaaca aattactgaa
    18901 attgttggtg gttccgcagc gcttgaataa tatttaaagg aggaaaatag catgggaatt
    18961 ggccgtgtaa ctcaagttat gggtcctgta attgatgttc gatttgaaca taacgaagtt
    19021 cctaaaatta ataacgcctt ggttattgat gtgcctaaag aagaaggtac aatacaacta
    19081 acattagaag ttgcgctgca attaggtgac gacgttgttc gtacaattgc gatggattca
    19141 actgatggtg tccaaagagg catggatgta aaagatacag gcaaagaaat tagtgtacct
    19201 gttggtgacg aaacattagg tcgtgtattt aatgtactag gtgaaacaat tgaccttaaa
    19261 gaagaaatta gtgattctgt tcgccgcgat cctatccatc gtcaagcacc agcattcgat
    19321 gaactttcaa cagaagttca aattttagaa acaggtatta aagtagtaga tttactagca
    19381 ccttatatta aaggtggtaa aatcggattg ttcggtggtg ccggtgtagg taaaacagta
    19441 ttaatccaag aattaattaa caacatcgct caagagcacg gtggtatttc tgtattcgcc
    19501 ggtgtaggtg aacgtactcg tgaaggtaac gatttatact tcgaaatgag tgacagtggt
    19561 gtaattaaga aaacagccat ggtattcggg caaatgaatg agccacctgg tgcacgtatg
    19621 cgtgttgcat tatctggttt aacaatggct gaatatttcc gtgacgaaca aggtcaagac
    19681 gtattattat tcatcgataa cattttcaga tttacacaag ctggttctga ggtatctgca
    19741 ttattaggtc gtatgccttc tgcagtaggt taccaaccaa cacttgctac tgaaatggga
    19801 caattacaag aacgtattac gtctacaaca aaaggatcag ttacttctat tcaagcggta
    19861 ttcgtacctg ccgatgacta tactgaccca gcgcctgcga cagcgtttgc ccatttagat
    19921 gcaactacaa acttagaacg taaattaact gaaatgggta tttatccagc cgtggatcca
    19981 ttagcgtcta catcaagagc attggaacca tcaattgtag gtcaagaaca ttatgaagta
    20041 gctcgtgatg tacaatcaac acttcaaaaa taccgtgaat tacaagatat cattgctatc
    20101 ttaggtatgg acgaattatc tgatgaagat aaacaaacag ttgaacgcgc acgtagaatt
    20161 caattcttct tatctcaaaa cttccacgta gcggaacaat ttactggtca aaaaggttct
    20221 tatgtacctg ttaagacaac agttgcaaac tttaaagata tcttagatgg taaatatgac
    20281 catattccag aagatgcatt ccgtttagtt ggtagcatgg atgatgttat tgcaaaagct
    20341 aaagatatgg gtgttgaagt ataacaatta ggaggaatgg ataatgaata cattaaacct
    20401 agatattgtc actcctaatg gttctgttta caatcgtgat aatgttgaac tcgttgttat
    20461 gcaaacaaca gctggtgaga taggtgtcat gagtggacat attccaactg tagctgcttt
    20521 aaaaacaggc tttgtaaaag tgaaatttca cgatggaact gaatatattg ctgtaagcga
    20581 tggctttgtt gaagttagaa aagataaagt ttcaatcatt gttcagactg cagaaactgc
    20641 aagagaaatt gatgttgaaa gagctaaatt agccaaagca agagcagagt ctcacttgga
    20701 aaatgatgac gacaatactg atattcatag agccgaaaga gctttagaga gagcaaataa
    20761 ccgtttgcgt gtggctgaat taaaatagta aataaagggt cgaagatgtg atttcatatc
    20821 ttcgaccctt ttttgaatta tattgattta aagatacaaa acatgagagg ggggaaggaa
    20881 ttgataaaga accattaaag atttatgatg tagtggttct ttatcattaa acacagctaa
    20941 tgtgtattta aaaataggaa tacatgagta aaactcatgt ataagaaata ctaatttcta
    21001 aagaaaaagt atttctttat gttggggccc cgcaaactac tgccaaatat aacactatag
    21061 agtctagaca ttgatttatg tccgactccc aagaatagtt ttatttttta caatcactaa
    21121 tagattgcta aaatcaaatt ttccttcacc actatctgca gtcgacgttt cattttttga
    21181 aagtagctac attttttcat accaagatac tttatagtta tgatatttat gtaaaaagaa
    21241 ttatatagta agttagctta aactttacta aaaacgggta ttaaactttg tatcattatt
    21301 taaatttttc atgtacaatg taatacagta atcttatgag gtgataaaat ggattatatc
    21361 ggacaatatg cagttatcca tttagtgtta catgttgtat gtatttgtat tgcctattgg
    21421 gctttacaat caattagatt agatcaattt tttaaaaaag gatacgccac tcaattacaa
    21481 gtgtgtatga tatttgttgc tattttatta ggcactgcag taagcaattt tattgtagat
    21541 ttgttacaat actcgacgca ggtaaaatat ttaataaaat aagtctaact ctatgatttg
    21601 taatcaaaac tagatataat taaataatga cttaaaataa ttttaaaata gggaaatgta
    21661 aagtaatagg agttctaagt ggaggattta cgatggataa aatagtaatc aaaggtggaa
    21721 ataaattaac gggtgaagtt aaagtagaag gtgctaaaaa tgcagtatta ccaatattga
    21781 cagcatcttt attagcttct gataaaccga gcaaattagt taatgttcca gctttaagtg
    21841 atgtagaaac aataaataat gtattaacaa ctttaaatgc tgacgttaca tacaaaaagg
    21901 acgaaaatgc tgttgtcgtt gatgcaacaa agactctaaa tgaagaggca ccatatgaat
    21961 atgttagtaa aatgcgtgca agtattttag ttatgggacc tcttttagca agactaggac
    22021 atgctattgt tgcattgcct ggtggttgtg caattggaag tagaccgatt gagcaacaca
    22081 ttaaaggttt tgaagcttta ggcgcagaaa ttcatcttga aaatggtaat atttatgcta
    22141 atgctaaaga tggattaaaa ggtacatcaa ttcatttaga ttttccaagt gtaggagcaa
    22201 cacaaaatat tattatggca gcatcattag ctaagggtaa gactttaatt gaaaatgcag
    22261 ctaaagaacc tgaaattgtc gatttagcaa actacattaa tgaaatgggt ggtagaatta
    22321 ctggtgctgg tacagacaca attacaatca atggtgtaga atcattacat ggtgtagaac
    22381 atgctatcat tccagataga attgaagcag gcacattact aatcgctggt gctataacgc
    22441 gtggtgatat ttttgtacgt ggtgcaatca aagaacatat ggcgagttta gtctataaac
    22501 tagaagaaat gggcgttgaa ttggactatc aagaagatgg tattcgtgta cgtgctgaag
    22561 gggaattaca acctgtagac atcaaaacac taccacatcc tggattcccg actgatatgc
    22621 aatcacaaat gatggcattg ttattaacgg caaatggtca taaagtcgta accgaaactg
    22681 tttttgaaaa ccgttttatg catgttgcag agttcaaacg tatgaatgct aatatcaatg
    22741 tagaaggtcg tagtgctaaa cttgaaggta aaagtcaatt gcaaggtgca caagttaaag
    22801 cgactgattt aagagcagca gcagccttaa ttttagctgg attagttgct gatggtaaaa
    22861 caagcgttac tgaattaacg cacctagata gaggctatgt tgacttacac ggtaaattga
    22921 agcaattagg tgcagacatt gaacgtatta acgattaatt cagtaaatta atataatgga
    22981 ggatttcaac catggaaaca atttttgatt ataaccaaat taaacaaatt atacctcaca
    23041 gacagccatt tttattaatt gataaagtag ttgaatatga agaaggtcaa cgttgtgtgg
    23101 ctattaaaca agtatcagga aacgaaccat tctttcaagg gcattttcct gagtatgcgg
    23161 taatgccagg cgtattaatt actgaagcgt tagctcaaac aggtgcggta gctattttaa
    23221 atagtgaaga aaataaaggt aaaatcgctt tatttgctgg tattgataaa tgtcgtttta
    23281 aacgtcaagt agtacctggt gatactttaa cgttggaagt agaaatcact aaaattaaag
    23341 gaccaatcgg taaaggtaat gctaaagcta ctgtcgatgg tcaacttgct tgtagttgtg
    23401 aacttacatt tgcaattcaa gatgtaaaat aaaacaaaaa aaacattcaa agatttaatg
    23461 tgttggcata atctttgaat gttttttatt ttactcttct aatttttcat cctttaactt
    23521 tggtttagac tgcatcattc gattaaatga tttttttaat tcttcaccag ataatccatc
    23581 atcaataagt tgttctaata aactttcagc atactgttga cgtaaagtgt aaatatgaca
    23641 ttcaaatacg atagacattg ttgttttaaa atttttaata cttttcacag tagactcaaa
    23701 taaagctgga tcattggctg gacgcgtgat gacgacacga atatccaata actgttcatt
    23761 gtcttgatat tccttaaatg tttcataatg ctcattagaa atgacaattt caagcaacca
    23821 acccgtgcca ctttcctctt tattaattat tacgccatct tctaattcat attctataat
    23881 tctgccgtgt tcattcacaa tttgaaacct tactgcttta aaagttttca tattcccaca
    23941 tcccattagt ttaatattta tgatttttga attatttaga agatttaagg tcatttcatg
    24001 actaatttct ttaaatataa caaataacga gatgctatag ctattaatta tataaaattt
    24061 gccattttga ccatttttaa gctagtaatt aagatatagg cataggaggt gaatcacaca
    24121 ctacatgcta aataaaatcg taattgtcgg gagactgacg aaagacgcac aaatatttga
    24181 aaaggaggat agaaaaattg caacgttttg tgttgcaacg caccgaaatt ataaagatga
    24241 aaatggagaa atcgtctgtg attacttatt ctgtaaagca tttggcaagt tagcttctaa
    24301 tatagaaaaa tatactaatc aaggtacatt ggttggtata actggtcaaa tgagatcaag
    24361 aaagtatgat aaagacggac aaacacactt tgtcactgaa ttatatgttg aaacaataaa
    24421 atttatgtcc cctaaatccc aaaataatga aattctctca gatagtattt tagatattga
    24481 ctctcaaaat atagataatc atgacttatt agaaatttaa atccaattat cctaaacatc
    24541 cttaatatac atttaatcca tcctaaattt tttatagatc taattaactt gaaatatact
    24601 cacttaaaaa cgacttacat gactagtact gttgtgtagg tcgtcttttt gtgtagtttt
    24661 tctaatagaa aatagacata gattgcaaaa cgattacata aaaaattgca aataaaaaac
    24721 atttttactg tcatgacaat tctcgataga atcatatctg aaattatggg ggatttcgtt
    24781 gtaatcactg tgtaaaaaac aaggctattt ttttattttg ttgtaacctg cacggatttt
    24841 acagatgtta cagtagtctc tgtaaaactt ttagagaaac aaattacttg taggaggatt
    24901 ttaagattat gaagaaaaca ttactcgcat catcattagc agtaggttta ggaatcgtag
    24961 caggaaatgc aggtcacgaa gcccatgcaa gtgaagcgga cttaaataaa gcatctttag
    25021 cgcaaatggc gcaatcaaat gatcaaacat taaatcaaaa accaattgaa gctggcgttt
    25081 ataattatac atttgactat gaagggttta cttatcactt tgaatcagat ggtacacact
    25141 ttgcttggaa ttaccatgca acaggtgcta atggagcaga catgagtgca caagcacctg
    25201 caactaataa tgttgcacca tcagctgttc aagctaatca agtacaatca caagaagttg
    25261 aagcaccaca aaatgctcaa actcaacaac cacaagcatc aacatcaaac aattcacaag
    25321 ttactgcaac accaactgaa tcaaaagcat cagaaggttc atcagtaaat gtgaatgctc
    25381 atctaaaaca aattgctcaa cgtgaatcag gtggcaatat tcatgctgta aatccaacat
    25441 caggtgcagc tggtaagtat caattcttac aatcaacttg ggattcagta gcacctgcta
    25501 aatataaagg tgtatcacca gcaaatgctc ctgaaagtgt tcaagatgcc gcagcagtaa
    25561 aattatataa cactggtggt gctggacatt gggttactgc ataagccatt tatgcatagc
    25621 taattaatag ttatataagt aacttttaga ttggaatata tcggggaatt ctcgatatgt
    25681 tccggttttt ttatttcgca aatatatgtc attatacagg ctgggacata aatcaatgtt
    25741 ctatgctcta cgaagttata ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag
    25801 cttttttcaa ttctagtcaa ccttgccggg gtgggacgac gaaataaatt ttgcgaaaat
    25861 atcatttctg tcccactccc aactgagcaa caataattga aaatgttgaa accgtttgct
    25921 aagattaatt tgcaattgaa tactaaggaa ctactagggg agcctaatga tatggctgag
    25981 atgaattgtt cagaccctta tgacctgatt tggttagtac caacgtagga aagtagttat
    26041 tgaagtgatg gggctagtat agcaacattg ccatcaaact actttcatgt tggaagtagt
    26101 ttttttattt agggaggatt ttttacaatg gaattttcac aaaaattgta ccaagctgca
    26161 aagccaatca ttaatgatat ttatgaggat gactttattc aaaaaatgtt attaggcaat
    26221 atacaagctg atgcattacg tcactattta caagctgatg cagcttattt aaaagaattc
    26281 acaaacttat atgctttgtt aattccaaaa atgaatagca tgaatgacgt aaaattttta
    26341 gtagagcaaa tagaatttat ggttgaaggt gaagtacttg cgcatgatat tttagcgcaa
    26401 attgtgggtg aatcatacga agaaatcatt aaaacaaaag tttggcctcc aagtggcgac
    26461 cattacataa aacatatgta tttccaagcg catagtcgtg aaaatgctat ttatacaatt
    26521 gctgcaatgg caccttgtcc atatatttat gcagaattag ctaagcgctc tcaaagtgat
    26581 cataagctta atagagaaaa agatacggca aaatggtttg atttttatag tactgaaatg
    26641 gatgacataa ttaacgtatt tgaggcatta atgaataaat tagccgaatc aatgtctgac
    26701 atggaattgg aacaagtaaa gcaagtattt ttagaaagtt gcatacatga gcgtcgattt
    26761 ttcaatatgg ctatgacact agaacaatgg gaatttggag gaaaagtaaa tgattaaacc
    26821 taaaatagca ttaaccattg caggtactga tccaacaggt ggtgccggcg taatggctga
    26881 tttaaaatca tttcattcat gtggtgtata tggtatgggc gtcgttacaa gtattgttgc
    26941 tcaaaataca ttgggcgtac aacatattca taatttaaat catcaatggg tagatgaaca
    27001 acttgatagt gtcttcaatg ataccttacc tcatgctatt aaaacgggga tgattgctac
    27061 agcagatact atggaaacga ttcgtcatta tttaatgcaa catgaatcta ttccatatgt
    27121 aattgatcct gttatgttgg cgaaaagcgg tgattcatta atggataatg acacaaagca
    27181 aaacttgcag catacgttat tgccattagc tgacgtagta acaccgaatt taccagaagc
    27241 tgaagaaata acgggactaa ccattgatag tgaagaaaaa attatgcagg ctggccgcat
    27301 ctttattaat gagattggta gtaaaggtgt catcattaaa ggcggtcatt caaatgatac
    27361 tgatatagca aaagattatt tatttactaa cgaaggtgtt caaacatttg aaaatgaacg
    27421 atttaaaaca aaacatacgc atggaacagg gtgtacattt tcagcagtta taacggcaga
    27481 acttgcaaaa ggtagacgat tatttgaggc tgtacacaag gctaaaaagt ttatttcaat
    27541 gagtatacaa tatacgcctg aaatcggccg tggtagaggt ccagtgaatc attttgcata
    27601 tttaaagaaa gagggattag acgatgaatt atctaaataa aatacgtatt gaaaacccat
    27661 taacaatttg ttatacaaac gatgtagtta aaaattttac agcgaatggt ttattaagta
    27721 ttggtgctag ccctgcaatg agtgaagctc ccgaagaagc tgaagaattt tacaaagttg
    27781 cacaagcgct attaatcaat atcggtactt taacagcaga aaatgaacaa gatattattg
    27841 cgattgctca aacggcaaat gaggcaggct tacctattgt atttgaccct gtagctgttg
    27901 gtgcttctac atatcgaaag caattttgta aattattatt gaaatcagcg aaagtatcag
    27961 taattaaagg caatgcatct gaaatattag cgttgattga tgatacagca actatgaaag
    28021 gtacagatag tgatgctaat cttgatgcgg ttgcaatagc gaaaaaggct tacgcaacat
    28081 ataaaactgc aatagtaatc acaggtaaag aggacgttat tgttcaagat aataaagcct
    28141 tcgtattagc taatggatct ccattattag cacgagtaac tggagctggt tgtttattag
    28201 gaggcgttat tgctggattt ttatttagag aaacagaacc agacatagaa gcgttaattg
    28261 aagcggtaag cgtatttaat attgctgctg aggtagctgc tgaaaatgaa aattgtggtg
    28321 gtcctggtac gttttcacca ttgttgcttg atacgttata tcatttaaat gaaacaacat
    28381 atcaacaacg gattcgtatt caagaggtgg aataatatgt ttaaccaatc gtatctaaat
    28441 gtgtatttta tttgtgggac ctccgatgtt ccgagtcatc gaactattca tgaagtatta
    28501 gaagcagcat taaaggcagg gattacactt ttccaatttc gagaaaaggg tgaatcagct
    28561 ttaaaaggaa acgataaact tgtattagct aaagaattac agcatctctg tcatcaatat
    28621 gatgtgcctt ttatcgttaa tgatgatgta tccttggcaa aagaaattaa cgcggatggt
    28681 atacatgtcg gtcaagatga tgcgaaagtc aaagagatag cacaatattt cactgataaa
    28741 attattggac ttagtattag tgatttagat gagtatgcaa aatctgattt aactcatgtt
    28801 gattatatag gtgttggacc aatctatcct acgccgtcga agtatgacgc gcatatacca
    28861 gtaggtccag aaatgattgc aacgtttaaa gaaatgaatc cgcaactacc gattgtagct
    28921 attggcggta tcaatactaa taatgtggcg ccgattgtag aagcaggtgc caatggaatt
    28981 tcagtcattt cagccatttc taaaagtgaa aatattgaaa agactgttaa tcgattcaaa
    29041 gattttttta ataattagtt aaatttttcc acttattgtt tcatacgtac tcattaatgt
    29101 gatagaataa atcctatgtg aatataatga tagaggtgga aaaatgaaga aaaaagcgtt
    29161 actaccatta tttttaggta ttatggtctt tttggctggt tgtgactatt ctaaacctga
    29221 aaaacgtagt gggtttttct acaatacatt cgtagatcca atgaaaaatg tattggattg
    29281 gttgggaaat aacttattaa acgacaatta tggtttagct attattatcc ttgtattggt
    29341 aattcgtatt attttattac cattcatgtt gtcaaactat aaaaatagtc atatgatgcg
    29401 tcaaaaaatg aaagttgcaa agccagaagt tgaaaaaatt caagaaaaag tgaaacgtgc
    29461 gcgtacacaa gaagaaaaaa tggctgcaaa ccaagaatta atgcaagtat ataaaaagta
    29521 tgacatgaac ccgattaaga gtatgttggg ttgtttacca atgctaatcc aattgccaat
    29581 catcatggga ttatactttg tacttaaaga ccaacttgta gatggtttgt ttaaatatcc
    29641 acacttctta tggttcgatt taggacgtcc tgatatttgg attacaatta ttgccggtgt
    29701 tttatacttt atccaagcat atgtatcaag taaaacgatg ccagacgaac aacgtcaaat
    29761 gggttacatg atgatggtca tttcaccaat tatgattatc tggatttcat taagctcagc
    29821 atcagcactt ggtttgtact ggtcagtcag tgcggcgttc cttgtagttc aaacacactt
    29881 tgcgaacatt tattatgaaa aagtcgctaa aaaagaagta caacctttca ttgaagcgta
    29941 tgaaagagag cacaacggcg gcagcaataa aaaaggcaaa aacacacaag tagtgtctaa
    30001 aaagaaaaag aaataacctt agcaagtcca aataatgtgc accctaaaag ttgaagataa
    30061 ttttaacttc atagggcgca cattattttt cgcttcaaaa gcgaaactta gtgtcacatt
    30121 atacaatatg tgataaatag aaacagcagt aagatgattt taaatgtgaa agtcatccta
    30181 ctgctgtttt tatttgaaat aatattaacg agttttcagt atgtaaaaag tggttaataa
    30241 cagagttgtc attcttcatc gcaatgtcat cttgacacaa tgattcatca gtgtagcagt
    30301 atatgtcgca aatttccaca gcaattcact aatacatgta acagtgtgaa atactaacat
    30361 cacaacggca taatacacac tgcaacgtcc caacaacata tcatagaata gtaacggcat
    30421 ggcatcaaca ccaaaccggg ctataatacc ataacagcgc aacgccacat catagcacta
    30481 catcaagtcg catcgtagca ccagcacatc aaagtaacat ggcatgacac caaacctgtc
    30541 tataacacca taacagcgca acgccacatc atagcactac atcaagtcgc atcgtggtac
    30601 cagcacatca aagtaacaag gcatcaacac caaacctgtc tataacacca taacagcgca
    30661 acgccacatc atagcactac atcaagtcgc atcgtggcac cagcacatca aagtagcatg
    30721 gcatcaacac caagctccca caacaacacg ccattacatc gcatcatcat actacaactt
    30781 caatgtctag tcgtgataat tccattccgt aaaaaactgt ttcaagtaca tcatcataaa
    30841 gtcgtgacgt tctttagcaa tcatcttcgc cgtatctgta tgcattaaag attctaactt
    30901 aagtaatttt tcaaagaaat gtttaattgc agatggtggc aactgttcaa ctatatcatc
    30961 attaatctta tctagtgaca tatgttctgt ccacattggt tcaccaaagt gtcctgcaaa
    31021 ttgaaatgtt cgtgcaacac ctatagcgcc tatagcatca agacgatctg catccctgac
    31081 aatttgacct tctaaagata aagtgacatg atcgtttttg ccattgcgat agctcatatt
    31141 attaataata aataaaatgt gctcttgatc ttcggttgat agtgataaag aagataaaaa
    31201 tgtcttcaat tcaacatatt gtttgttagc atctacaact ttttcgtcaa cggtatcatg
    31261 aagcaaacat gcgagttcaa tgactaaagt atcattaaca ccttcatttt cagcgattga
    31321 tttagctaaa gcagtgacac gatatacatg cgcaatatca tggcctgtat aatcattttg
    31381 atgaatagat tccatatatt ttttggcagc actaatgacg tctgattgtt gcattaaagt
    31441 tacactcctc atatttctat tttataagat aggtgacaat aattgtgaaa taccttcttt
    31501 aaatttaatc caaagacttc gcttagcata acgtgctttt gttaattcag aagatactgc
    31561 taaatcatct ataaaagctt gttttaattt tttggcaatt tgttggtcat aaataaaggc
    31621 gttgacttcg aaattcaatg tgaaactgcg atggtccata ttagctgttc caacacttgc
    31681 aatttcatca tctataacaa gtgtttttga gtgtaaaaag ccattgtcgt agtgaaatac
    31741 tttaacaccg gcatctaata aggatgctgc attttttaaa gtagcccaaa aaacaaacgg
    31801 atggtcaggt ttattaggaa tcatgatatt gacatcaaca ccacctaatg ccgcaatttt
    31861 aatagaatct aaaaaggctt gatcaggtat aaaataggga gattgaatat aaatcgattt
    31921 tttagcagat gaaatcattt tcaaataacc gtatttaatc tgttcccatt cttcgtcagg
    31981 accactagaa gctatttgaa cgccaattgt tccaccagaa tttacatctg ggaaataacg
    32041 atcatcatag gagatgtggt cacgtgtggc ttgtgaattc caatctagaa taaatcgtaa
    32101 ttgcaatgca ttcactgcgt ccccgacaat tcgtaaatgc gtatctcgcc aatagccgaa
    32161 tttttttgat ttacctaagt actcatcacc aacattaaaa ccaccaacat atccaatttg
    32221 cccatctatt acaacaattt ttcgatggtt tcgattgttc atacgcaagt taattaaagg
    32281 taattttgat gggaaaaatg cttcagcatg tccaccttta ttgcgaaacg ggcgtaagcc
    32341 ttttttacgc agtccacgag aacccatgtc atcataaaga attttaactt ctacaccttg
    32401 ttccgctttt ttaccaagtt catttaaaat ggtacgacct aattcatcat tttgaataat
    32461 atagtactga aaatgaatat aatcagtagc attacggatg tcttgtatta ggtcatcaaa
    32521 tttttcttgg ccgtctgtgt atatttttaa atcgttgtct gttgttaaaa atgctgcgtt
    32581 attatataac aacatttgaa tcatttcttt aaattttaca atttgataat tggaatttga
    32641 aaagttttca ttttttaaag cagctaattg ctcatcaacg attaactcta atcctttttt
    32701 atcttcctta tcaattttga aaatttggtc acgttgaatt tgtcgtccta atagtaagta
    32761 taaaatgaag ccgaataaag gcaagaaaac taagactagt aaccaagccc agatagaatt
    32821 ggcagaacgt ctttccatga aaataatggt aaaggcgaat aataagttta aaataaatgc
    32881 accaataaat attgaattta aaataatatt agaatgcttg agtgcaatgg ataataactc
    32941 tatcatttga aacctcccat cgaaaatctt aaacgagtag tatatcttat taattgtatc
    33001 atgaagcaat ttaaatatca tgacaattta gtttgaaaaa cacactttgt gttgacgttg
    33061 tgttttatgg gatatagtat agtaaggaat gtaaatgaag gagtgaatgc tatgactgaa
    33121 caagataatg cacatcattc tgaacaaata aaaacgaatc ttaaatcacg tttaaatcga
    33181 attgaaggac aagtgagagc gattaatcgc atgattgaag aagatgtcta ttgtgatgat
    33241 gtccttacgc aaataagagc gacacgttcg gcgttaaaca gtgttgcgat aaagttatta
    33301 gaacaacata tgaaaagttg tattatgaat aaagttaatc aaggtgctca ggaagaggca
    33361 atggaagagt tattagtgac ttttcaaaaa ttgattaaag actaaaggag tttaagatga
    33421 tacatcaaaa tacgatttac acagcgggaa ttgaaacaga agaacaagta agtcaattga
    33481 cagaacgcat ttcaaatatg ataggtgttc atcaagtgaa tattaatata atagatggtc
    33541 aagtaactgt atcgtatgag acaccagcaa atttgaatag tattgaaaaa gaaatctatg
    33601 atgaaggata caaaattgta ttttagggta taatgtagag tgcgctatag attttaattt
    33661 tgaaaataaa ttaaaaattt tgtaaatgat gtagtaaagg tatgtcgaat agggtaagat
    33721 aatattgaaa gcgatccaaa cgaggtgata ataatgaaac gtcctgaaaa gattcaaaat
    33781 gtagtcaaac tattgtcatc attaggtgtg aatattaaaa aaactaaatc tcgtttagac
    33841 attattaata ctttgccagc atctaataaa gtaagtcacg aattaaaata attgactgtg
    33901 attgtgaatc acacaccgtt aaagctatcg tcggactatt cgatgatagc ttttttgact
    33961 ggatttacta aaatgaaagc aagtactata taacaaaatc cctctttaga gtgtacattt
    34021 tacaatgtgt acttcaaaga gggattgtta ttatcgagta atatctatat atgacatttt
    34081 aaatcgacga cgtaaataga ctatataacg tcatcttgaa aagttatgat atttgaaact
    34141 caaaatagtt taaattaatt actttttgga tggtataaat cgacatatcg ttttggttca
    34201 tgataataaa ttgataagac tatacctatt ccagtcatca tactccatag cgcactacca
    34261 ccataactaa taaatggtaa tggaatacca gtgattggta acaactgaat tgtcatacca
    34321 atattttgta aaatatggaa cacaagtaaa gtgacgaaac caacgataaa gattttgtta
    34381 aattgatctt caattttcgc agctaatctt attagatgga agattaaaaa taaaaatatt
    34441 aagatcaata tgacagaacc gataaagcca agttcctctc caatcactga aaagataaag
    34501 tcagtatgat tttcaggtat ataaacttca ccgtgattgt atcctttacc tagtaactgt
    34561 ccagaaccga tagctttaag tgattcagtt aaatgatagc catcaccact actatatgta
    34621 taggggtcaa gccatgaatt gattcgtccc atttgataca gttggacacc taataaattt
    34681 tcaattaatg cgggtgcata tagaatacct aaaatgactg tcattgcacc aacaatacct
    34741 gtaataaaga taggtgctaa gatacgccat gttataccac ttactaacat cacacctgca
    34801 ataatagcag ctaatactaa tgtagttcct aggtcatttt gcagtaatat taaaatactt
    34861 ggtactaacg agacaccaat aattttgaaa aataataaca aatcactttg gaatgattta
    34921 ttgaatgtga attgattatg tctagaaacg acacgcgcta atgctaaaat taaaataatt
    34981 ttcatgaatt cagatggctg aatactgata gggccaaacg tgtaccaact tttggcacca
    35041 ttgataatag gtgtaatagg tgactcagga ataacgagca agcctattaa taatagacag
    35101 attaagaaat acaataaata tgtataatgt ttaatctttt taggtgaaat aaacatgatg
    35161 atacctgcaa aaattgcacc taaaatgtaa taaaaaattt gtctgatacc gaaattagca
    35221 ctgtattgac caccgcccat tgccgagtta ataagcagaa cactgaaaat tgctaaaaca
    35281 gctatagtgg ctactaatac ccagtctact ttgcgaagcc aatgcttatc cggctgttga
    35341 cgagatgaat aattcattgc aaactccttt tatactcact aatgtttata tcaattttac
    35401 atgacttttt aaaaattagc tagaatatca cagtgatatc agctatagat ttcaatttga
    35461 attaggaata aaatagaagg gaatattgtt ctgattataa atgaatcaac atagatacag
    35521 acacataacg cctcgttttt aaaatgcaaa atagcattaa aatgtgatac tattaagatt
    35581 caaagatgcg aataaatcaa tcaacaatag gactaaatca atattaattt atattaaggt
    35641 agcaaaccct gatatatcat tggaggaaaa cgaaatgaca aaagaaaata tttgtatcgt
    35701 ttttggaggg aaaagtgcag aacacgaagt atcgattctg acagcacaaa atgtattaaa
    35761 tgcaatagat aaagacaaat atcatgttga tatcatttat attaccaatg atggtgattg
    35821 gagaaagcaa aataatatta cagctgaaat taaatctact gatgagcttc atttagaaaa
    35881 tggagaggcg cttgagattt cacagctatt gaaagaaagt agttcaggac aaccatacga
    35941 tgcagtattc ccattattac atggtcctaa tggtgaagat ggcacgattc aagggctttt
    36001 tgaagttttg gatgtaccat atgtaggaaa tggtgtattg tcagctgcaa gttctatgga
    36061 caaacttgta atgaaacaat tatttgaaca tcgagggtta ccacagttac cttatattag
    36121 tttcttacgt tctgaatatg aaaaatatga acataacatt ttaaaattag taaatgataa
    36181 attaaattac ccagtctttg ttaaacctgc taacttaggg tcaagtgtag gtataagtaa
    36241 atgtaataat gaagcggaac ttaaagaagg tattaaagaa gcattccaat ttgaccgtaa
    36301 gcttgttatt gaacaaggcg ttaacgcacg tgaaattgaa gtagcagttt taggaaatga
    36361 ctatcctgaa gcgacttggc caggtgaagt cgtaaaagat gtcgcgtttt acgattacaa
    36421 atcaaaatat aaagatggta aggttcaatt acaaattcca gctgacttag acgaagatgt
    36481 tcaattaacg cttagaaata tggcattaga ggcattcaaa gcgacagatt gttctggttt
    36541 agtccgtgct gatttctttg taacagaaga caaccaaata tatattaatg aaacaaatgc
    36601 aatgcctgga tttacggctt tcagtatgta tccaaaatta tgggaaaata tgggcttatc
    36661 ttatccagaa ttgattacaa aacttatcga gcttgctaaa gaacgtcacc aggataaaca
    36721 gaaaaataaa tacaaaattg actaactgag gttgttatta tgattaatgt tacattaaag
    36781 caaattcaat catggattcc ttgtgaaatt gaagatcaat ttttaaatca agagataaat
    36841 ggagtcacaa ttgattcacg agcaatttct aaaaatatgt tatttatacc atttaaaggt
    36901 gaaaatgttg acggtcatcg ctttgtctct aaagcattac aagatggtgc tggagctgct
    36961 ttttatcaaa gagggacacc tatagatgaa aatgtaagcg ggcctattat atgggttgaa
    37021 gacacattaa cggcattaca acaattggca caagcttact tgagacatgt aaaccctaaa
    37081 gtaattgccg tcacagggtc taatggtaaa acaacgacta aagatatgat tgaaagtgta
    37141 ttgcataccg aatttaaagt taagaaaacg caaggtaatt acaataatga aattggttta
    37201 cctttaacta ttttggaatt agataatgat actgaaatat caatattgga gatggggatg
    37261 tcaggtttcc atgaaattga atttctgtca aacctcgctc aaccagatat tgcagttata
    37321 actaatattg gtgagtcaca tatgcaagat ttaggttcgc gcgaggggat tgctaaagct
    37381 aaatctgaaa ttacaatagg tctaaaagat aatggtacgt ttatatatga tggcgatgaa
    37441 ccattattga aaccgcatgt taaagaagtt gaaaatgcaa aatgtattag tatcggtgtt
    37501 gctactgata atgcattagt ttgttctgtt gatgatagag atactacagg tatttcattt
    37561 acgattaata ataaagaaca ttatgatcta ccaatattag gaaagcataa tatgaaaaat
    37621 gcgacgattg ccattgcggt tggtcatgaa ttaggtttga catataacac aatctatcaa
    37681 aatttaaaaa atgtcagctt aactggtatg cgtatggagc aacatacatt agaaaatgat
    37741 attactgtga taaatgatgc ctataatgca agtcctacaa gtatgagagc agctattgat
    37801 acactgggta ctttgacagg gcgtcgcatt ctaattttag gagatgtttt agaattaggt
    37861 gaaaatagca aagaaatgca tatcggtgta ggtaattatt tagaagaaaa gcatatagat
    37921 gtgttgtata cgtttggtaa tgaagcgaag tatatttatg attcgggcca gcaacatgtc
    37981 gaaaaagcac aacacttcaa ttctaaagac gatatgatag aagttttaac aagcgattta
    38041 aaagcgcatg accgtgtatt agttaaagga tcacgtggta tgaaattaga agaagtggta
    38101 aatgctttaa tttcatagag attagtcgag ggacctttta cttataaaaa tgatttgaat
    38161 taatactaaa agattacaaa gaagaggtgg ttttgtgtgt aaatacaaaa ttgccttttt
    38221 ctttttatgt taaatctata aatttgaaac taaatcaagg ttaattctat gtacacactt
    38281 tatataggaa gtagtttgaa tgtttatata atgttttaca aaaagatgta gtattataat
    38341 gtctaatttc acatgtgttt cagtaaaatt tgttgtggaa tgttaacgat atacgtattt
    38401 tataaaaaat tttttataat gattattcga atgatgcgta acgcttacat cttatctaat
    38461 gctagctttt tgacaaaaat atgacaatca attaatgtga ttctaataaa tattcgcaaa
    38521 ttgctttatt gcgattaaat ttttttggtg gtactatata gaagttgatg aaatattaat
    38581 gaacttatat gcaaaagtat attgagaaat aaacaggtaa aaaggagaat tattttgcaa
    38641 aattttaaag aactagggat ttcggataat acggttcagt cacttgaatc aatgggattt
    38701 aaagagccga cacctatcca aaaagacagt atcccttatg cgttacaagg aattgatatc
    38761 cttgggcaag ctcaaaccgg tacaggtaaa acaggagcat tcggtattcc tttaattgag
    38821 aaagtagtag ggaaacaagg ggttcaatcg ttgattttag cacctacaag agaattggca
    38881 atgcaggtag ctgaacaatt aagagaattt agccgtggac aaggtgtcca agttgttact
    38941 gtattcggtg gtatgcctat cgaacgccaa attaaagcct tgaaaaaagg cccacaaatc
    39001 gtagtcggaa cacctgggcg tgttatcgac catttaaatc gtcgcacatt aaaaacggat
    39061 ggaattcata ctttgatttt agatgaagct gatgaaatga tgaatatggg attcatcgat
    39121 gatatgagat ttattatgga taaaattcca gcagtacaac gtcaaacaat gttgttctca
    39181 gctacaatgc ctaaagcaat ccaagcttta gtacaacaat ttatgaaatc accaaaaatc
    39241 attaagacaa tgaataatga aatgtctgat ccacaaatcg aagaattcta tacaattgtt
    39301 aaagaattag agaaatttga tacatttaca aatttcctag atgttcatca acctgaatta
    39361 gcaatcgtat tcggacgtac aaaacgtcgt gttgatgaat taacaagtgc tttgatttct
    39421 aaaggatata aagctgaagg cttacatggt gatattacac aagcgaaacg tttagaagta
    39481 ttaaagaaat ttaaaaatga ccaaattaat attttagtcg ctactgatgt agcagcaaga
    39541 ggactagata tttctggtgt gagtcatgtt tataactttg atatacctca agatactgaa
    39601 agctatacac accgtattgg tcgtacgggt cgtgctggta aagaaggtat cgctgtaacg
    39661 tttgttaatc caatcgaaat ggattatatc agacaaattg aagatgcaaa cggtagaaaa
    39721 atgagtgcac ttcgtccacc tcatcgtaaa gaagtacttc aagcacgtga agatgacatc
    39781 aaagaaaaag ttgaaaattg gatgtctaaa gagtcagaat cacgcttgaa acgcatttct
    39841 acagagttgt taaatgaata taacgatgtt gatttagttg ctgcactttt acaagagtta
    39901 gtagaagcaa acgatgaagt tgaagttcaa ttaacttttg aaaaaccatt atctcgcaaa
    39961 ggccgtaacg gtaaaccaag tggttctcgt aacagaaata gtaagcgtgg taatcctaaa
    40021 tttgacagta agagtaaacg ttcaaaagga tactcaagta aaaagaaaag tacaaaaaaa
    40081 ttcgaccgta aagagaagag cagcggtgga agcagaccta tgaaaggtcg cacatttgct
    40141 gaccatcaaa aataatttat agattaagag cttaaagatg taatgtcttg agctcttttt
    40201 tgttttcaat aattgattct ctgtagatat caaagtacta acgttttaaa ggttaaatat
    40261 ttaattggat tgagatctgt atgcggttat atcattctgt gtaaatataa atatgaatct
    40321 agaatataac aactacttaa attaagaata atcgaacata aataaacttt atgaaatttc
    40381 agtatcatgt tcttgtaaaa aaataatagg gctttttgct gacgctagta cgtgataaat
    40441 aataagttga atataaaaaa gatcactgcc aatcattcgt ttaatagcag cgatcttttt
    40501 tatttaatta tttctctttc cactgcaaca tttgataacc aatgcgtgga tgtgttttaa
    40561 taatatcttt tgcgtcctca tgacattgtg aaagtttttg tcttaatgat gtcatatgca
    40621 ctcttaaact aggcatttca gtcttattaa catagccata tattcgtttt aatatcatct
    40681 cataagtgag tacttttcct ttatgatttg acaatagttc taacaagcta aattcatttg
    40741 gagtcaaatg tacctcctga ttattaataa cgacagattt ggagccaaag tcgatgctta
    40801 gcaaaccgtt agtaaataca atgttagttt cttgatgtga cttagcgatt ctctcgatga
    40861 ctcgtattcg tgcccgaagc tcatcaacat taaaaggttt agtcatatag tcattcgcac
    40921 cgttatctaa agcttgaata attgtttgtt cttcttgtct tgcacttatt acaatgatag
    40981 gaatgtcagt atgttgcctg atttctgaaa tcaaacataa tccatcttta tctggtaaac
    41041 ctaaatctaa taaaatgaca tctggtttat caatttgaat tttaaagtgt gcttgtgtgg
    41101 cattgtcggc tgtagttaca ttgtaataat ctaaagttaa tgcaacatca agtaaatgtg
    41161 tgattgcgtg atcatcttca attatcaata ttttagattg cattatacgt ctccttcatt
    41221 aaagtctgta tatatattga aatagaatat actgccgtgt ggttggttcg gtttatattg
    41281 taagtttgat tgatgtttgt gtaggatagt ctgtactaaa tataagccta gtcccatgct
    41341 ttctttttgg ttatctttaa aatatttatt tgatcctgtg taaaaaggct cgaatatctt
    41401 ttgttgttct tctaaactaa ttccaggtcc ttcgtctata acggcaaatt cgatttgttc
    41461 atagctagca taacgaatag ataaattgat tttggtgtca gtagaagtgt gtttaactgc
    41521 attttcaatc aaattgaata aagcttgtaa aatcaactta ctgtcaatgt gtataaactg
    41581 taaatttact gaggatgata cagttatacg cttttttaaa tggcgacgtt ctaaaatcat
    41641 atcgatttct tctactaatt cactaacgag ataaggttgc aattttatct gaacattgga
    41701 tgactgtaat tttgttaatg ataaaatatt tgtcactaat agatataaat actgactttc
    41761 ttgaaaacta tgcacaagta attgttcctt ttctatgata gacatatctt tactatgtga
    41821 tactaaaata tctaaatttc ccataattgt tgttaacggt gtacgtatgt catgcgaaat
    41881 tgatcttaaa aaatttgaat gtgtcagttg acgttcagcc tgtaacatgg attctctcgt
    41941 ttgtttaagt aacgtcacat tttcaacggc gagagaaagt tcatttaaca ttgattctaa
    42001 tattgatgca tcatatggat taatcacttg agaactttgg taatcaatgg ctagaatgcc
    42061 tttaatcgga gatgtgccaa ttggtatcaa ccatttatta atgcctggaa atgtatctgt
    42121 tgttgcacca gcttgtcttt catttttaat tacccagctt aatgcttgtt catgctgttg
    42181 agtcgtatta tcgatatggt tttgcaatgg tattgtttta attactttcg attgattgat
    42241 aacgtatata gtaattgatt gttgcaataa ttgattaatt tggtatccag catttattag
    42301 taagttttca actgtataag tttgtttaat cgaatcatta aattgaaata ataaatctgt
    42361 acgataaagt tgctttttag taatggagta ttggaattta atttgtttta ataaagcact
    42421 cgttaaaata cttgttaaaa tgctaacgat aaatgtaata ggatagtcaa agcgatatac
    42481 ttcaaatgta tatctaggtt ccgtaaaaaa ataattaaaa acaaatacgt taataattgc
    42541 tgctaaaaag ccaatgatga aggaccgcgt ccaaatggat aataaaatga tgccgatgag
    42601 aaaaatcatt aaaataatcg tactagactc atgcttatca agttgataaa tccacagtcc
    42661 catcattaca caaattattt gaatcaaaat catttttaac gtatctatgg cgaaacgttt
    42721 gcctttagga cgatagggtg atttgttcat tttcaattca acaggtattt gtttgattgg
    42781 aacgatttcg attttatagc tatgttcaaa ggacattaaa tggtcaatta aaggtgtatt
    42841 gaaaaagtct cgccacttat ttctaatatg ttgtccgata attaatttgg ttacatcttg
    42901 atttttacac cattcgtcta atcctaatgc aacggtttgg ctataaacta cttttacttt
    42961 tgctcctaat gattttgcaa gcatgagatg ttgatgcact tgcttttgac tatctttata
    43021 ttgcctgttt ttttcgaata catctatata aatagcagtg aacttcgcat gttctttttg
    43081 agcaatatgg aatgcctctt taattactgc ttcattataa atgctcccac taattgccac
    43141 agcaatatga ggtttgagtg acgttttatg gttgtgtcgg actttttctt tatcactcat
    43201 caagtcggca actgttctta acgtcaatgt acgcagttcg cttaggtggg catacgtaaa
    43261 gaaattacta aatgctacat ctagcctatc ctttttatat accttgccag cttttaagcg
    43321 tttaattaat tgttcaggtg agatatctac gacttctaat acatcggcgc tcattatgaa
    43381 atagtcgggt acacgttctt taacatgtac accggtcatc agttcaattt gactacttaa
    43441 actttcaata tgttgaatgt tcaaagtggt atgaacatcg ataccatgat ttaaaatttc
    43501 ttcaatatcc atatatcgtt tctcatgacg atctctagaa atattcgtat gtgctaactc
    43561 atcgataagt actattgtcg gcgattcttc gattatgcgg tcgacatcta aatattgaaa
    43621 atgatgacta ccatgtttag taaaattggt tgtgatttta ggtaattgtt cagctaatgc
    43681 atttgtttca tcacgctgat gcggttcaat atatcctatt ttaatatcaa cgttactttg
    43741 aaatagttca atggcatttg aaagcatctc aaatgtttta ccaacacctg gactgtaacc
    43801 gatgtaaatt gttaaagatc cacgcttttt tcctatgttt agcgattcag tgtttgacat
    43861 aaccttcacc tcgatagcaa tatcataata ttcttcacaa ttaaatgcta taaaatttat
    43921 ttctgcatac acatcttaat gatttcttaa taactaagtt tgaaaattaa tatttctctc
    43981 ataaacaagt tgttacgatg ttgttgtgtt gtaaggaggt tatggcaatg ctcatcgttt
    44041 tattactggt agttattgca ttagttttgt atttatttta tgccttaatt cgtagtgaaa
    44101 aattttagaa atatattaag tgagggttat catggaaata attttgtttc taacaatgat
    44161 ggttatgatt acgtatgttt ttagtggata tctatatcgc gtagcattgg tgcaatcgtc
    44221 aagagtagat ttaatattta ctagatttga aaatatgtgt tttaaaatta tcggcacaga
    44281 tttagaacac atgtcagcta aaacatatgt caaacatttt ctggcattta atggatttat
    44341 ggggtttata acatttgtat tgctaatagt tcagcaatgg ctttttttaa atccaaatca
    44401 taatttaaac caatcgatag atttagcgtt taatacagca atatcttttt taacaaatag
    44461 taatttacaa cactataacg gtgaatcaga tgtgacatat ttaacgcaaa tgattgtaat
    44521 gacatattta atgtttacat ctagtgcatc aggttacgcc gtttgtatag cgatgttgag
    44581 acgtttaact ggattaacta atatcattgg taatttttat caagatattg ttcggtttat
    44641 tgtccgagta gttttaccat tatcatgttt aattagtatt ttattgatga ctcaaggtgt
    44701 accacaaacg ttgcatgcta atttaatgat tcggacttta agcggacata ttcaacatat
    44761 tgcatttgga cctattgcat cacttgaatc aataaaacat cttggtacga atggtggagg
    44821 atttttagca ggaaattctg caacaccttt tgaaaatcca aatatttgga gcaattttat
    44881 agaaatgggc agtatgatgt tacttcctat gtcaatgttg ttcttatttg gtcgcatgtt
    44941 aagtagacat ggtaaacgag tacatcgtca tgcgttgata ttatttgtcg caatgttttt
    45001 catttttata gcaattctta cattaactat gtggagtgag tatcgtggta atccaatact
    45061 agcgaattta ggcatttatg gaccgaatat ggaaggtaaa gaggtacggt ttggagcagg
    45121 cttgtcagca ctatttacag ttattacgac ggcatttaca acgggttctg ttaataacat
    45181 gcatgatagc ttaacgcctt taggtggatt aggaccaatg gtattaatga tgctaaatgt
    45241 tgtatttggt ggcgaaggcg taggactcat gaatttattg atatatgtct tactgacggt
    45301 gtttatatgc agtttgatgg ttggtaaaac accagaatat ttaaatatgc caattggcgc
    45361 ccgtgaaatg aaatgtattg tcttagtctt tctcatacat ccaattttaa ttttagtatt
    45421 ttcagcactt gcttttatga ttcctggagc aagtgaaagt ataacgaatc cgtcttttca
    45481 tggtatttcg caagttatgt atgaaatgac atcagctgct gcgaacaatg gatcagggtt
    45541 tgaaggactg aaagatgata caacattctg gaatatctct acaggaatca ttatgttgct
    45601 ttctcgttat ataccaatta ttttgcaatt aatgattgca tcaagtttag tgaataaaaa
    45661 atcataccac caagataaat atacaatagc gattgataaa ccttattttg gcgtatcact
    45721 aatcgtattt atcgttttac taagtggttt gacatttatt ccagtactat tacttggtcc
    45781 tattggtgaa tttttaactt taaaataaag taaagggatg gaaaaaatgc atcatgtaaa
    45841 taaatatttc aatcaaacaa tggtgataga agctttgaaa atgagttttt ataagctgaa
    45901 tcctaaacaa ttaataaaaa atccgataat gtttgtcgtt gaggttggaa tgattttaac
    45961 gttgatatta atatgttttc ctgacatatt tggaactagt tatctatcgc gaggttattt
    46021 gattacaatt tttatcatat tattaataac tatattgttt gctaattttt cagaagcctt
    46081 tgctgaaggt cgtggaaaag cgcaagctga tagtttaaga caagcacaat caaatttaac
    46141 tgctcgattg atagaagaaa atggtgcgta tcggatagtg aacgccacag agttaaaagc
    46201 cggacaaaat attcgggtag aaaatggaga aacaatccct gttgatggcg ttgtaataaa
    46261 tggattagca accgttgatg aatctgcaat tactggtgag tctgcacctg tgataaaaga
    46321 atcaggtggc gattttgatg gagtcattgg cggtacactt gtaacatctg attggttaga
    46381 aatacgtgtt gagagtgaag cgggtacatc ttttttagat aaaatgattg ccttagttga
    46441 aggggcggaa agaaataaaa caccaaatga aattgcactt tttacattat taacaacgtt
    46501 aacaattata tttttagtcg ttattgtaac gttatatcca atagcatctt acctgcattt
    46561 aattttacct attgcgatgc tcattgcgtt aacagtatgt ttaataccaa cgacaatcgg
    46621 tggtttgtta tcggcgattg gtattgcagg catggatcgt gttactcaat tcaatgtatt
    46681 agcgaaaagt gggcgtgctg tagaagtatg tggagatgtc gatgtaatga ttttagacaa
    46741 gacaggcaca atcacttatg gcaatcgaat cgctagtgaa tttttaccag tgaatcaaca
    46801 aatgctggaa aaattgatag tcgctgctta tatgtcatca atctacgatg atacgccaga
    46861 aggaaaaagt attgtacgat tagcgaagca gatgtatata tatgagctgc ctaaagacat
    46921 tgatggcaca tataagactt ttacagctga aactagaatg agcggaatca ttacaaatga
    46981 aatatcggta ttcaaaggtg cacctaatag tatgataaat cttgtcaaac aacaacaagg
    47041 caatattcct cttaatattg agtctctctg tatggatgta tcgagtaaag gtggcacacc
    47101 attaatagtc attgaaaaca atgtcatgct tggcgtgatt tatttaaaag atgtgattaa
    47161 agatggtctt gtagaacgat ttactgaatt gagaaaaatg gggattgaaa cagttatgtg
    47221 tacaggtgat aatgctttaa cagcagctac aattgcaaaa gaagccgggg ttgatagatt
    47281 tgttgcagag tgtaaacccg aagataaaat taaagtcatc aaagatgaac aagcgaaagg
    47341 ccatattgtt gcgatgacag gagatggtac aaatgatgca cctgcgctag cacaagcgaa
    47401 tataggatta gctatgaact cagggacaat tagtgctaaa gaggcagcta atctaattga
    47461 tttagattcg aatcctacaa agttaataga ggttgtaaaa ataggcaaac aattattaat
    47521 gacgagaggt gcattaacga catttagttt ggctaatgac gtagcaaaat actttgcaat
    47581 tttacctgca ctaatgatgt caacaattcc agaaatgact tcactaaata ttatgcattt
    47641 atcgtcacct aaatctgcaa ttatatcagc tttgatattc aatgcattaa tcattgttgc
    47701 cttaattcca attgccatga aaggtgtaaa agtaaagggt tacagcattg atagaatttt
    47761 tataaataat atgttgattt atggattagg tggattaatc gtcccgtttt taggcattaa
    47821 attaatagat atgattgtac aattttttgt gtaaagagga aggagcctgt accatgaata
    47881 cgataagaaa tagtatttgt ttaacgataa taactatggt attatgtgga tttttattcc
    47941 cgttagctat cacgttaatt ggtcaaatat ttttttatca acaagcaaac ggcagtttaa
    48001 ttacgtatga caatcgtata gtaggatcaa aattgattgg acagcactgg acagagacac
    48061 gatattttca cgggagaccg agtgcagttg attataatat gaatcctgaa aaattatata
    48121 aaaacggcgt atcatcaggt ggaagtaatg aatcgaatgg aaacaccgaa ttaatagcac
    48181 gcatgaaaca tcatgttaaa tttgggaatt caaatgtaac aatagatgct gcaacaagtt
    48241 ctgggtcggg cttagatccg catattacgg ttgaaaatgc attgaagcaa gcacctcgta
    48301 ttgctgatgc tcgtcatata tctacttcaa gagtggccga cttgattcaa catcgtaagc
    48361 agcgtggtgt gttaacaaat gattatgtga atgtgcttga actaaatatt gctcttgata
    48421 agatgaaaga ttgattatag atgaaaggca tcatgttgat aaaattataa catggtgtct
    48481 ttttagcgat tcaagcactt tctgcttaac taaactttta gatgtacctt taggaagaat
    48541 aaattagcat gaaatattcc atatgctata attagcaaca tataaaaata aaatgaaata
    48601 gattataaat ccgtataatt ttttatttgg ggagaggggt atatgtcaga ttatactaag
    48661 ttgtctaaga gtcctaagtc agcattgttg tattattaca ttacgaatgg cttagaattt
    48721 atattgagtg tagctgtgta tgtcattttt tattttatat ggttaagatt tgaatggcca
    48781 cattacttac tttatatttt gttggtttta ggtgctctga gtacattaaa attatgtatt
    48841 aagcctttat ggcaatatca ttgtcgcttt tataaagttg atcaactaag tgtacaatat
    48901 cgtacatcat ttttaatata taaagaagaa acgagtcgaa ttgaacgttt acaatacttg
    48961 tcgattaaat ctaatccaat tagtaaagtg cttaatttgt ataaagttgg atttatgact
    49021 gcaggtcata ctatttattt accgatgatg tctcatgacg atgttaaaat aatcgaagcg
    49081 agaactatgt cgaatttgag aggggttgaa agtgatgtct gaacctcaaa aattacatcc
    49141 gatttcatat tttaatggca taatcaatgc tatcaaacaa aacatagtcg tcttttttat
    49201 tttcattatc tttcaattga aagattttga ttatacgaat cccgaatcat atttatggat
    49261 aggtattgta tttgtattct ttttaatatc atacatttct caaattgtcg gaattatgaa
    49321 cacgcgatat tggattgagg ataattattt cattttaaca acaggtattt ttaataaaaa
    49381 gcgtaaagaa ttaaatataa aacgtattca gtccgttgat atgacacaag gggtcgtcaa
    49441 tcaaattatt ggtggtgtgg atttacaaat caaaacacca agtgatggta ttgtcttaag
    49501 tgttatttcg aaaaaacaag gtgaatatct tgaacgatat attgatcaat tgcaaacaga
    49561 gttgaagaca gaagtggcgc aaaagtctag tgctgagaag aatgacaact gtaatcatga
    49621 cattgaaggt aatgtggaag acatcgcaga aggtcgatta gatgaagtgc agaacactga
    49681 tagtatgctt aatcagaaaa ataaacgcat tcctatatat cagatgaatt ttaaagaatc
    49741 actatttatg gcaatgacca gtggcgccat tggtgtaacg ttagcagctt tagtaccgat
    49801 ttacggggct gtaagagagt tgatcccatg gtcgaagcta aatcatgaaa ttagtcaatg
    49861 ggttcaaatt gtaagtattt cagtattaat catcatttta ctagtattaa tagtggcata
    49921 tattattgga acgattataa cgatgataag attttatggc ttttcggtga tgcttgaaaa
    49981 tgaacaactt aaaattgaat atggtctctt taacaagaag aaagtaactg taccaacgaa
    50041 acgattgcaa gctgttgttg aacgtcagtc atatatacga agattatttg gctacacagc
    50101 aatacatttt atcatcacaa gtgattttga aaataaaaaa atcgaagatg atgacaatga
    50161 ttcaggtagt gtgccgatat tgccgtttat taaacgagac aaagcatatc aaattatcca
    50221 gaatctagta ccagatttat cttatcaaca agttaatgaa ggtatgcctt tgtcaggttt
    50281 ccacagacac tttttaatac ctagtattat tgtcatagct atggcaggaa ttggctgtta
    50341 tttttggaat caattattat tgttatttac gattataaca gtagtcatta ttggtttatt
    50401 tgtgatcaaa ggatatcttt atgtcaaaca tgcgggtttt aaaatgacag aagaacaaat
    50461 tactatccag aatttcgaac tgttgaaacg aaatatttat tattttaaag cgcgtcacat
    50521 tataggattt gacacttggc aacatacatt acttaaacgc aacaatttaa ataattttag
    50581 ttttattgtt gctaaaggtg tgggagccca atcgattaat ttaaaatacg caagaagtga
    50641 agatgcaaat aaattgaaat catggtattt aagaggtgaa aatgatgagg gattataatt
    50701 ttatgtcacc acacgctaaa aaagtgatga ggttaagtgc tgtgctgttt tggataccca
    50761 tagcgcttgt acttttaacc gcatttaata ttttgaactg gctattttgg gagattttag
    50821 ataactatat atccattgtt agtagtgtga ttataatatt acttgttgct gttttcacgc
    50881 ttgtcatagc gccagtatat cgatataagc actgcagata tgcatttgaa aaacaccatt
    50941 tgcgtgtacg aaacggcatt ttatttttgg atgaaaaagt aataccatat tttcgtattc
    51001 aaaatattga tatcgatgtt ggtcctataa tgagacgcta tcgattagca actttgaccc
    51061 tttatacagc gggtggtcaa gctgaaattg aattaataga tagaagtgaa gcacgtagat
    51121 tgaaacgatg gttaaataac gagagacatt tagaaaacaa taaaaatgag gaataatcaa
    51181 tgatacatgg aattggtgta gatttaatcg aaatcgatcg aatacaattg ttatatagta
    51241 agcaaccaaa attggttgag cggattttaa ctaaaaatga acagcacaaa ttcaacaatt
    51301 tcacacatga gcaacgtaaa attgaatttt tagctggcag gtttgctaca aaagaagcgt
    51361 tcagtaaagc attaggcaca ggcttaggaa aacatgtagc ttttaacgat atagactgtt
    51421 acaacgacga acttggcaaa ccaaagattg attacgaagg gtttatcgta catgttagta
    51481 tctcacacac tgagcattat gcgatgagcc aagttgtttt agaaaagtca gcattttaaa
    51541 tttgttatac aatgataatt aagatctagt tttaaaacgt gtgggaatta caggaggaag
    51601 taatatgtcg aataaatatt atagatctgc gtatatgaat gtagatttaa acgctgttgc
    51661 atcaaatttc aaagtattca gtacattgca tccaaataaa acagtgatgg ctgtcgttaa
    51721 agccaatgcc tatggactag gtagtgttaa agtagcacgt catttaatgg aaaatggcgc
    51781 cacatttttt gctgtagcaa cgttagatga agcgatagaa cttagaatgc atgggattac
    51841 tgctaaaatt ttagtcttag gtgtgttacc agctaaagat attgataaag cgatacaaca
    51901 ccgagttgcc ttaacggttc cgtctaaaca gtggttgaaa gaagcaatta aaaacatttc
    51961 tggtgagcaa gagaaaaagt tatggttgca cattaaatta gatacaggaa tgggacgttt
    52021 aggtattaaa gatactaaaa cgtatcaaga agtgattgaa atcattcaac aatatgagca
    52081 acttgtattt gaaggcgtgt ttacacactt tgcctgtgct gacgaaccag gagatatgac
    52141 aactgaacaa tatcaacgtt ttaaagatat ggtcaatgaa gcaattaaac ctgaatatat
    52201 acattgtcag aactcagcag gttctctact aatggattgc caattctgta atgcaataag
    52261 accaggaatt tccctttatg gatattatcc atcagagtat gtacagcaaa aagttaaagt
    52321 acaccttaaa ccaagtgtgc aattaattgc taatgtagtt caaacaaaga cgctacaagc
    52381 gggtgagtct gtaagttatg gtgcaactta tacagctact gacccaacta caatagcatt
    52441 gttacctatt ggatatgcag atggctattt acgcataatg caaggtagct ttgtaaatgt
    52501 aaatggtcat caatgcgaag ttattggtcg cgtatgtatg gatcagacaa ttgttaaagt
    52561 gccagatcaa gttaaagctg gagattcggt gattttaata gataatcata gagaaagtcc
    52621 acagtcggta gaggtggtag ctgaaaagca acatactatt aattatgaag tgctttgtaa
    52681 cttgtcgaga cgtttgccgc gaatctatca tgatggtgat caacgttttg taacaaatga
    52741 attgttaaaa taatatggtc agtgcatata aaatttgtta tcattagagt aattaaaggt
    52801 catttaataa cttttggaat caattggagg ttctcatatg ttatctttta gtcaaaatag
    52861 aagtcatagc ttagaacaat ctttaaaaga aggatattca caaatggctg atttaaatct
    52921 ctccctagcg aacgaagctt ttccgataga gtgtgaagca tgcgattgca acgaaacata
    52981 tttatcttct aattcaacga atgaatgatt agacgaggag atgtttattt agcagattta
    53041 tcaccagtaa agggatctga acaaggggga gtcagacctg tagtcataat tcaaaatgat
    53101 actggtaata aatatagtcc tacagttatt gttgcggcaa taactggtag gattaataaa
    53161 gcgaaaatac cgacacatgt agagattgaa aagaaaaagt ataagttgga taaagactca
    53221 gttatattat tagaacaaat tcgtacactt gataaaaaac gattgaaaga aaaactgacg
    53281 tacttatccg atgataaaat gaaagaagta gataatgcac taatgattag tttagggctg
    53341 aatgcagtag ctcaccagaa aaattaggcg tct
//
LOCUS       DAHHYJ010000002        52103 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000002 DAHHYJ010000000
VERSION     DAHHYJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 52103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52103
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.002"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..700)
                     /locus_tag="NBK22_000058"
     CDS             complement(<1..700)
                     /locus_tag="NBK22_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413282.1"
                     /translation="MGKPLFKKGDSMNNSIFYTYLKFNLRYFIRRKFEIVKRSRLNVT
                     TKILFLIKLIFSSLLLFISTIMLADYFSDYIDFIEYYFYASMFIYIVFIPLIPRTKLL
                     INPMDKQLLYRSSLREVDIFNLIYASDVLKKFVGYFNLLIVGMAISLYHISPFIFNLK
                     VFCALIYFSTISYVIQIIFINIKVISTKKLFSYHYCVFNFMMGSIVSVIGFVVSFLLV
                     NLLIKPFLVFSTYVI"
     gene            complement(738..959)
                     /locus_tag="NBK22_000059"
     CDS             complement(738..959)
                     /locus_tag="NBK22_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cyclic bacteriocin"
                     /protein_id="HCD2413283.1"
                     /translation="MLKLHKKIAWTGIKGSAITTFITALYNGSSVWNALAVAGIAFGG
                     GAGTAVAALGRATVMRFIKRWGVRKTAAW"
     gene            complement(1166..2344)
                     /locus_tag="NBK22_000060"
     CDS             complement(1166..2344)
                     /locus_tag="NBK22_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2413284.1"
                     /translation="MRKVFNIFTIAFKSILKNKGRNIFTMIGIIIGISSVITIMSLGN
                     GFKKTAADQFSDAGAGKQEALISFTFKVDEKIKKYPFNQRDIELVNQVDGVLDAKLKE
                     NKEEGIEATITNVQKKSDIFIIKKQNLHSFKVGRGFDKEDNELRKKIVVINDQVAKTV
                     FNNNAIGKSLYIEGQGFEVIGITDKLYSDSSTVIMPENTFNYYMGHLHQGLPTLQIII
                     EDGYNKKTVVKKVESLLNKKGSGSVLGEYTYTDTEEIIKSIDKIFDSITYFVAAVAGI
                     SLFIAGIGVMNVMYISVAERTEEIAIRRAFGAKSRDIELQFLIESILICVTSGFIGLI
                     LGVVFATIIDVLTPDYIKSVVSLSSVIIAVSVSILIGLLFGWIPARAASKKELIDIIK
                     "
     gene            complement(2341..3021)
                     /locus_tag="NBK22_000061"
     CDS             complement(2341..3021)
                     /locus_tag="NBK22_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2413285.1"
                     /translation="MIELKNVYRTFKNGDKNIRVLKDINIHINAGEFIAIMGPSGSGK
                     STLINILGFIDREYHGEYLFEGNNYQTQSDNQLANIRNKTVGFVFQNFKLIHNNTILE
                     NISMPLVYTNMNTIERKNKVMTVLSAVGLDGKGDFTPSKLSGGQQQRVAIARAIINNP
                     KFIIADEPTGALDSDTSKIIMDIFTSLNRDNHTTIILVTHDRKVAEKADRIIHILDGR
                     IQHEEVIK"
     gene            complement(3018..4130)
                     /locus_tag="NBK22_000062"
     CDS             complement(3018..4130)
                     /locus_tag="NBK22_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCD2413286.1"
                     /translation="MRRKRKWLIVICVLLVLITISQILKHFNYGTESKEAYNTYTVEK
                     ERQLDLQGKAYPREVKTYYKNNQVGTYLDVQVADGQTVKKGDMLINYKINSNKRQQLV
                     DKINDTQHIITEVYLNITKGLNNREIQKKLIEYKQTLYEAQQQLYQYDSQNRDSMYAS
                     FDGKIMIKNIDNDSDNQPILYLISKESHIKTSVTEFDLNKVKKGDKVNLSITSTGKTG
                     AGIIKQISELPISYEENLSLHENDALHLPESNNDGELANSKSISASPIFKSNNNSELS
                     KYGVIIDDLSLPIRAGYSLEIKVPLNVIKIPKSVLTKGNNVFIVNKNSIVEKRNIRIK
                     RINGDIIVEKGLKPGDKLIKQPKSTMNDGDKVEISS"
     gene            4256..4855
                     /locus_tag="NBK22_000063"
     CDS             4256..4855
                     /locus_tag="NBK22_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413287.1"
                     /translation="MSLSKLPLFNHFANIRIQPKLIIKLFLTLIIIVTYTFVSGLSVD
                     YKKLLSEAGLSETSLAQIKIFSQISIFFSRVFSVLFTFLIILVISRIMRSDANTKSIF
                     SATLSYILFTNAISLIILTIQISAGLSLIDYSITSLNIFAKGNRILGTFDLQFFVQAY
                     ILTVMLIGTSKLSIRASTIWGIIYLILIITFSLISISIQ"
     gene            complement(5003..7792)
                     /locus_tag="NBK22_000064"
     CDS             complement(5003..7792)
                     /locus_tag="NBK22_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD2413288.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYVWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTVAGIFTFKPNEDDCDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKPFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQYSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
     gene            8005..8286
                     /locus_tag="NBK22_000065"
     CDS             8005..8286
                     /locus_tag="NBK22_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413289.1"
                     /translation="MVLITYQIILFFIISLSYYLTLNHYMAVTVGNFTSIFGMFAAIL
                     FMYYYLLYKSPEYNQRKRFKHFIHITNLIIIAFSTFVLVHLALKLFFSI"
     gene            complement(8480..9355)
                     /locus_tag="NBK22_000066"
     CDS             complement(8480..9355)
                     /locus_tag="NBK22_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD2413290.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
     gene            complement(9358..10812)
                     /locus_tag="NBK22_000067"
     CDS             complement(9358..10812)
                     /locus_tag="NBK22_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2413291.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKLNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            complement(10824..11720)
                     /gene="murQ"
                     /locus_tag="NBK22_000068"
     CDS             complement(10824..11720)
                     /gene="murQ"
                     /locus_tag="NBK22_000068"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCD2413292.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            complement(11720..12775)
                     /locus_tag="NBK22_000069"
     CDS             complement(11720..12775)
                     /locus_tag="NBK22_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCD2413293.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYDHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSDLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            13361..15406
                     /locus_tag="NBK22_000070"
     CDS             13361..15406
                     /locus_tag="NBK22_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCD2413294.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKIQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK"
     gene            complement(15385..15486)
                     /locus_tag="NBK22_000071"
     CDS             complement(15385..15486)
                     /locus_tag="NBK22_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413295.1"
                     /translation="MMPQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            15679..17319
                     /locus_tag="NBK22_000072"
     CDS             15679..17319
                     /locus_tag="NBK22_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCD2413296.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTAAKDTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNN
                     ATFPAYHRPTSPDYTVGTAPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            17386..17943
                     /locus_tag="NBK22_000073"
     CDS             17386..17943
                     /locus_tag="NBK22_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCD2413297.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPHTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNVLN"
     gene            18219..19574
                     /gene="brnQ"
                     /locus_tag="NBK22_000074"
     CDS             18219..19574
                     /gene="brnQ"
                     /locus_tag="NBK22_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD2413298.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKLNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKLIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIYKIKHRKIPQ
                     A"
     gene            19827..21011
                     /gene="rocD"
                     /locus_tag="NBK22_000075"
     CDS             19827..21011
                     /gene="rocD"
                     /locus_tag="NBK22_000075"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCD2413299.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRHLCDKYQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDKEEIHQIVAAFQDVF
                     KN"
     gene            21050..22081
                     /locus_tag="NBK22_000076"
     CDS             21050..22081
                     /locus_tag="NBK22_000076"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCD2413300.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHVTIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     IAGQDVSVIFTPHLVPMTRGILSTIYVKFSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETSQTAILVSVIDNLVKGASGQAIQNLNILYDFEVTT
                     GLNQSPVYP"
     gene            22093..23334
                     /gene="argJ"
                     /locus_tag="NBK22_000077"
     CDS             22093..23334
                     /gene="argJ"
                     /locus_tag="NBK22_000077"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCD2413301.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSAKGSGMIHPNMATMLAFITTDASIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILNQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPKCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHEHVTIDVQLGLGNAA
                     ATAYGCDLSYDYVRINASYRT"
     gene            23350..24114
                     /gene="argB"
                     /locus_tag="NBK22_000078"
     CDS             23350..24114
                     /gene="argB"
                     /locus_tag="NBK22_000078"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCD2413302.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRLNNIYPIIVHGGGPFI
                     NDALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            24382..24861
                     /locus_tag="NBK22_000079"
     CDS             24382..24861
                     /locus_tag="NBK22_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCD2413303.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(25189..25833)
                     /locus_tag="NBK22_000080"
     CDS             complement(25189..25833)
                     /locus_tag="NBK22_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCD2413304.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMLQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(25846..33021)
                     /locus_tag="NBK22_000081"
     CDS             complement(25846..33021)
                     /locus_tag="NBK22_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCD2413305.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPSSSANYDLEAFFKQELS
                     TYHFNDSPLFKFKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHINRNDDKDNHDASHIALDSNYFRLENNSDIHIDSYLPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCAKSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTFLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINVSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     ATPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIALEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGERRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSG
                     LNVAQLQQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYTINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEVVSRQGNHVALVMNDLTMTYETLRNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPKKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGDNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPAQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQMEQDLKQYLNDHLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMHQNQQSLVALP
                     DNLSELQKIVMSRYNLGVLEDSLSHRPLGNTLLTGATGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDVIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSMAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLYHWAQYIKTIFNK"
     gene            complement(33468..34718)
                     /locus_tag="NBK22_000082"
     CDS             complement(33468..34718)
                     /locus_tag="NBK22_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2413306.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGIFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKERNYMLLISGFSIIMMGEFSISSYIAIRLKDQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGAFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            complement(35104..36129)
                     /locus_tag="NBK22_000083"
     CDS             complement(35104..36129)
                     /locus_tag="NBK22_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCD2413307.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GKDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            complement(36303..36662)
                     /locus_tag="NBK22_000084"
     CDS             complement(36303..36662)
                     /locus_tag="NBK22_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCD2413308.1"
                     /translation="MNTIDTHIKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            complement(36879..37910)
                     /locus_tag="NBK22_000085"
     CDS             complement(36879..37910)
                     /locus_tag="NBK22_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCD2413309.1"
                     /translation="MTLETLIKEQLDPHLVEVDEGTYYPRTFIQQLFVDGYFGEAALR
                     KNAEVIEAVSQSCLTTGFCLWCQLAFSTYLENATQPHLNNDLQQQLLSGEILGATGLS
                     NPMKSFNDLEKLNLEHTYVDGQLVVSGRMPAVSNIQADHYFGAISKHESSDEFVMFIL
                     RANQDGITLVEKTNFLGVNGSATYQITLNQVVVPQSQIITHDAKQFAATIRPQFIAYQ
                     IPIGLGSIKSSLELIDAFSNAQNGINQYLEYDVEAFKKRYRQLREEYYAILDDGNLTS
                     HLSELISLKKDIGYLLLDVNQASVVNGGSRAYTPYSPQVRKLKEGFFFAALTPTLRHL
                     GKLEAELKG"
     gene            complement(37923..38684)
                     /locus_tag="NBK22_000086"
     CDS             complement(37923..38684)
                     /locus_tag="NBK22_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2413310.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            complement(38681..39655)
                     /locus_tag="NBK22_000087"
     CDS             complement(38681..39655)
                     /locus_tag="NBK22_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2413311.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKNTQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLNQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            complement(39669..40409)
                     /locus_tag="NBK22_000088"
     CDS             complement(39669..40409)
                     /locus_tag="NBK22_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2413312.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            complement(40751..41263)
                     /locus_tag="NBK22_000089"
     CDS             complement(40751..41263)
                     /locus_tag="NBK22_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCD2413313.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            41442..41645
                     /locus_tag="NBK22_000090"
     CDS             41442..41645
                     /locus_tag="NBK22_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413314.1"
                     /translation="MDKESVVASLARNKKIAVETMAGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDSIKEIDENHLNDAT"
     gene            complement(41707..42666)
                     /locus_tag="NBK22_000091"
     CDS             complement(41707..42666)
                     /locus_tag="NBK22_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD2413315.1"
                     /translation="MNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(43312..44799)
                     /locus_tag="NBK22_000092"
     CDS             complement(43312..44799)
                     /locus_tag="NBK22_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCD2413316.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDHAVKVAQEAFESWSLTSKSERAQMLRDIGDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEVFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            45225..45608
                     /locus_tag="NBK22_000093"
     CDS             45225..45608
                     /locus_tag="NBK22_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCD2413317.1"
                     /translation="MRLILIVIGLIFTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            45615..45941
                     /gene="isdI"
                     /locus_tag="NBK22_000094"
     CDS             45615..45941
                     /gene="isdI"
                     /locus_tag="NBK22_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCD2413318.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(46006..47151)
                     /gene="cap8P"
                     /locus_tag="NBK22_000095"
     CDS             complement(46006..47151)
                     /gene="cap8P"
                     /locus_tag="NBK22_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCD2413319.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK"
     gene            complement(47228..48490)
                     /gene="cap8O"
                     /locus_tag="NBK22_000096"
     CDS             complement(47228..48490)
                     /gene="cap8O"
                     /locus_tag="NBK22_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCD2413320.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEIYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMRALD
                     SILPFLEKENTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNIALA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVELDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            complement(48544..49431)
                     /gene="capN"
                     /locus_tag="NBK22_000097"
     CDS             complement(48544..49431)
                     /gene="capN"
                     /locus_tag="NBK22_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCD2413321.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGHVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHRKTVLINMPSMLNKYFNKLSVFRKLFGNLIYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            complement(49431..49988)
                     /gene="cap8M"
                     /locus_tag="NBK22_000098"
     CDS             complement(49431..49988)
                     /gene="cap8M"
                     /locus_tag="NBK22_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCD2413322.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            complement(49999..51204)
                     /gene="cap8L"
                     /locus_tag="NBK22_000099"
     CDS             complement(49999..51204)
                     /gene="cap8L"
                     /locus_tag="NBK22_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCD2413323.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKALVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            complement(51238..>52103)
                     /gene="cap8K"
                     /locus_tag="NBK22_000100"
     CDS             complement(51238..>52103)
                     /gene="cap8K"
                     /locus_tag="NBK22_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8K"
                     /protein_id="HCD2413324.1"
                     /translation="FTNYLNKERKYKVLSLINVFRAGSMALLQIIFGLLALGSLGLII
                     GFSLSYIAGITLGYKTFKKHFNIVRDKEETKALFLENKNQLVYSTPSILLNSLSFSVV
                     VFFIGILYTNTEVGIYGMAIRVLGIPVTIISLGLSKIFMQQANDYYIEHGNFRNLLLK
                     FSSILVIVSIILYVPLYLFSEELVNILLGHSWVDAITVIKIVIPLFVIRLIVSTVSLS
                     VIVLQKQQLELILQALFLIGTTATFVISKMLNLTFLNFVSINTIVLIVSYMIFFIALY
                     YFAKNKQFKNS"
BASE COUNT        16695 a         9793 c         7774 g        17841 t
ORIGIN      
        1 ttattacata tgtcgaaaaa actaaaaagg gctttattaa taaatttact aaaagaaatg
       61 agacgacaaa tccaataaca gaaacaatgc ttcccatcat aaaattaaaa acacaatagt
      121 gataagaaaa taattttttt gtgcttatga ctttaatatt tataaaaata atttgaatta
      181 cataagagat tgttgaaaag tatattagtg cacaaaaaac cttgaggtta aatataaaag
      241 gactaatatg atataaacta atagccatac caactatcaa caaattaaaa tatccaacaa
      301 acttttttaa aacgtctgaa gcatatatca agttaaaaat gtcaacttct ctcaaactag
      361 atctatataa cagctgttta tccattggat tgatcaataa tttagttcta ggtattaatg
      421 gtataaatac aatgtaaata aacatactcg catagaaata atattctata aaatcaatat
      481 aatcactgaa atagtcagct aacataatag ttgaaataaa taataatagt gacgaaaaaa
      541 tcaatttaat taaaaacagt atttttgtag taacattaag tcgcgacctt tttacaattt
      601 caaattttct acggataaaa tatctaagat taaattttaa ataggtataa aatattgaat
      661 tattcattga atctcctttt ttaaaaagag gctttcccaa aaaagaaagc ctctcttata
      721 aagaattaga ttatctttta ccaagcagct gtttttcgta cgccccaacg tttgataaat
      781 ctcattactg ttgcacgacc aagagctgca accgcagtac cagcgccacc accaaaagca
      841 ataccagcaa cagctagcgc gttccaaaca ctacttccat tatataaggc agtaataaat
      901 gtagttatag ctgaaccttt gattcctgtc caagctattt ttttatgtaa ttttagcata
      961 ggccttccac ctccttctca aaaatattat ataatatttt tttatatctt aaaagtaata
     1021 tgttattttt tgcgtaaatt caatatataa tttacatttt gcaatacata tcttactttt
     1081 attctaatac gcatttccat cgttggtgtt gatagtcagt ttaatatgat aattagtctt
     1141 cagtactttt accattttaa atttactatt ttataatatc tatgagctct ttcttagatg
     1201 ctgcgcgagc tggtatccat ccaaaaagaa gtccaattaa tatcgacaca ctaaccgcaa
     1261 ttatgacaga actcaaactt actacacttt taatataatc tggtgttaat acatcaatta
     1321 ttgttgcaaa cacaacacct aaaataagtc caataaaacc acttgtcaca catattaaaa
     1381 tgctctctat taaaaattgt agttcaatat ctcgactttt tgcaccaaat gcacgtcgta
     1441 tcgcaatttc ttctgttctt tcagctacag atatatacat gacattcata actccaatac
     1501 cagcaataaa aagtgaaata ccggcaacag ctgctacaaa ataagtaata ctatcaaaaa
     1561 ttttatcaat acttttgata atttcttcag tgtctgtata tgtatactca cctaaaacag
     1621 agcccgatcc ttttttattt aatagtgatt ctactttttt aactacagtt tttttattgt
     1681 aaccatcttc aataattatc tgtaaggtag gcagaccttg atgtaagtgt cccatatagt
     1741 agttgaatgt attttcaggc attataacgg tagatgagtc agaatatagt ttatccgtaa
     1801 ttcctatgac ttcaaatccc tgtccctcaa tatatagcga tttaccaata gcattattat
     1861 tgaatacagt ttttgctact tgatcattta taaccactat tttcttacgt aattcattat
     1921 cctctttatc aaaccctcta cctactttaa aagagtgtaa attttgtttt tttataatga
     1981 agatgtcact ttttttctgg acattagtta ttgtggcttc aattccttct tctttatttt
     2041 cctttagctt cgcatctagt acgccatcaa cttgatttac caactctata tctctttgat
     2101 taaaaggata ctttttgatt ttctcatcaa ctttaaaagt aaagctaatc aacgcttctt
     2161 gttttccggc gccagcatct gaaaattgat cagcagcagt ttttttaaag ccatttccca
     2221 aagacattat cgtaataaca gaagaaatac cgatgattat acctatcatt gtaaatatat
     2281 ttctaccttt atttttcaat attgatttaa aagctattgt aaaaatattg aaaactttcc
     2341 tcatttaatc acctcttcat gttgtatacg accatccaaa atatgaatta ttcgatcagc
     2401 tttttctgct actttacgat catgtgtgac taaaataatt gtagtatgat tatctcggtt
     2461 taatgatgta aatatgtcca taataatttt agatgtatct gaatccaaag cacccgttgg
     2521 ctcatctgca ataataaact ttggattatt tataatggct cgcgcaattg caacacgttg
     2581 ttgttgaccg cctgatagtt tactaggagt aaaatcacct ttgccatcta ggcctacagc
     2641 actgagcaca gtcataactt tattctttcg ttctatcgta ttcatatttg tatataccag
     2701 tggcatactt atattttcta aaatagtatt attatgaatt agtttaaaat tttgaaaaac
     2761 aaatccaact gttttatttc ttatgtttgc caattgatta tcagattgtg tctgataatt
     2821 atttccttca aacaaatatt caccatgata ctctcgatct ataaatccta agatattaat
     2881 cagtgtgctc ttccctgagc ctgatggtcc cataatcgct atgaattcac ctgcattgat
     2941 atgtatatta atgtctttta aaactcgaat atttttatca ccgtttttaa aagtcctata
     3001 aacattcttt aattctatca tgacgaaatc tcaaccttat caccatcatt cattgtactt
     3061 ttaggttgtt taattaactt gtctccaggt tttaacccct tttctacaat aatgtcacca
     3121 ttgattcttt ttatacgaat attccgtttt tccacaatac tatttttatt aacaataaaa
     3181 acattattgc cttttgttaa tactgattta ggtattttaa tcacatttaa tgggacttta
     3241 atttccaacg aataacctgc acgtattggt agactaagat catcaattat aactccatac
     3301 tttgataatt cagagttgtt attactctta aatattggag atgccgaaat cgattttgaa
     3361 tttgctaatt ccccatcgtt gttactttct ggtaaatgca gggcatcatt ttcgtgaaga
     3421 cttaaattct cttcataact aataggcaac tcagagattt gttttataat ccccgctcct
     3481 gttttacccg tactcgtaat acttaagtta actttgtctc cttttttgac tttgtttaaa
     3541 tcaaactccg taacagacgt tttaatatga gactcctttg aaatgagata cagtataggt
     3601 tgattatcac tgtcattatc tatattcttg atcataatct tgccatcaaa tgatgcatac
     3661 atactatccc tattttgact atcatactga tacaattgct gttgagcttc atataatgtc
     3721 tgcttatatt caattaactt cttctgtatc tctcgattgt ttaaaccttt agtaatgttt
     3781 aaataaactt ctgtaataat atgttgagta tcatttatct tatcaacaag ttgctgtcgt
     3841 ttattactat ttattttata attaattaac atgtctcctt ttttgactgt ttgcccgtcc
     3901 gctacttgca catccaaata tgttccaacc tgattatttt tataatatgt tttaacttca
     3961 cgtggatatg ccttaccttg taaatctaat tgtctttctt tttcaaccgt atatgtattg
     4021 tatgcttctt tactctcagt tccatagtta aaatgtttca aaatttgtga tatcgtaatc
     4081 aatactaata atacacaaat aacaattagc catttccttt tacgcctcaa tttatgtcct
     4141 cctataaatg ttaaatactc ttcaaaccat tttttctact tagttttaat aaaatatatt
     4201 ttttatatta tttttatgat aaaatatagg agaattatat cattaggagg tctatatgtc
     4261 attatcaaaa ctacctttgt ttaatcactt tgcaaatatc cgcattcaac caaagttaat
     4321 cattaagctg tttctgactt tgataataat tgttacatat acatttgtta gtggactatc
     4381 tgtggactat aaaaagttat tgagtgaagc aggactctca gagacatcat tagcacaaat
     4441 aaaaattttt agtcaaatta gtattttttt ctcaagagtt ttcagcgtat tatttacatt
     4501 tttaatcatt ctagttatct ctagaattat gcgctcagat gctaatacaa aatctatatt
     4561 ctctgcaacg ttaagctaca tactgtttac aaatgctatt agtttaatta tacttaccat
     4621 ccaaataagt gccggtttat cattaattga ctattctatt actagtttaa atatttttgc
     4681 taaaggaaat cgcattctgg gtactttcga cttacaattt tttgtacaag catacatact
     4741 tacagtcatg ctaattggta cgtcgaaact ttctatacga gcatccacta tttggggtat
     4801 tatatatctt attttaatta tcactttttc tttgattagt attagcattc agtgatattt
     4861 tcagataaaa tgtctctatt aattaataag tttaaaacaa ggaacattcg ttatattctt
     4921 aataagatga atttaacaaa tgagcctttt ctaatgaaat aataatgccc ccctacacta
     4981 atctagcgag ggggctgaat cattacacac cgtatttttc agttgtttct gccacgaaag
     5041 attcaatggc ttttcttgct ttacgttttt ctttcaatgg caaatcacca atcatttttt
     5101 taaggtgatg tgggtttaca acaccactat actggtagtc atttgaaatt gtttttaggg
     5161 cttgttcatc gatagatctc tctcctgcaa accctttgaa ctccgcttct tttttaatac
     5221 tttcgaaatt aacatattct tgatcgatat catcatcctt atttaaagaa ggtacaacat
     5281 tgtcgatgaa ttctctaatt agatctcgtt ttaacctcaa tgtcggatca tctgcatgat
     5341 ctaaaatacg tctaatttgt tcttggttac gacgttgttc cgccttgtct tcaagatcaa
     5401 tttgtctcaa tatattcata atataattca cattaatcgt atcattacgc atcatttcta
     5461 tttcgaaatc aatatcattt aaaatggata ctttattttt ctcagccgtc gctcttttta
     5521 cttgatcgta cacagctaaa tatttacttt tatagtcttc attctcttgt tcatccattc
     5581 caatttcatc aattgtaaac tcaaactcgt caaatgcttt taaacgtaat attattttag
     5641 ctaataaacg ataagcttca acaaagcgct ttagctcttc ttcatcttga atgtcatcaa
     5701 ccatgtgtgg tgtcggcaca atcattttaa gctcacgata agcgtccata aattcttttt
     5761 tatactcttc ataactgcgc attaaaattg tatccgtatc atttgtttgt gagaatactc
     5821 tcagtgcatc gtctgtctct tttttcaagt cacgatagtt tacaatttta ccaaatggct
     5881 ttgattcttt ttcaacccta tttgtacgtg aatacgcttg aattaaatca tgatacatta
     5941 aattcttatc aacatataaa gtattcagta ctttactatc aaaaccagtt aagaacatat
     6001 taacaacgat taagatatca attttactat ctttaacgcc ctttttaacg ttttttgaaa
     6061 tatgattaaa atactcatta gtcgtgtctg ttgaaaaatt cgtctcgaac tttttattat
     6121 aatcgctaat cattatctct aatttttcac gtgaatgata tggcacttca ccatcacaat
     6181 catcttcatt aggtttaaac gtaaatatac cagctaccgt taatggttgt tccaactttt
     6241 tgttaagtcg cttaaatgtc tcataatatt taataagcgc gtgaatactt tggactgtaa
     6301 atatacttga atattgacga ttacgtgtat atttatcatg attattgatg atatgtcgtg
     6361 ttactaattc cacacgttta tccgctaacc atacttcttc cgtatcaatt gcttcaacca
     6421 tgctgttatc ttctgctttt aaagctttat ttttaaaagt attaatatag tcaactgaga
     6481 aaccaagtac attaccatca tgaatggcat ctctaattaa atatgtatgt aagcatctac
     6541 cgaaaatatc tgcagttgtt ctaccatctt gactactatt ttctggaaaa cgtggcgtac
     6601 cagtgaatcc aaagtattgg gcatttttga aatgttgttt aactagacga tgcatgtcac
     6661 caaattgact gcgatgacac tcatcaataa taaatacaac tttattcgtt ttatactgtt
     6721 ctaataaatg ggcattccct tgaatcgctt tagccatttt ttgaatcgtc gttacaataa
     6781 gtggcaaact tttatcattt agttggcgta ccagttgcga ggtattaaaa gttttgtcta
     6841 cagcaccctt agcaaattta ttaaattcct cttctgtttg actatccaag tctttacggt
     6901 caaccaaaaa gataactttc ttaatgtcat cttgctgtga taaaatctga ctcgctttaa
     6961 aagaagtcaa cgtcttacca cttccagttg tatgccatac atatccatta ttccctgtct
     7021 cagtcgcttg ttgaataagt gcttctaccg catacacttg atacggacgc attgccatca
     7081 gtattctatc tgtttcatta ataatcatat agcgcgatat catcttagct aattgacaag
     7141 gtctcataaa tgactcagca aacgattgca atgtattgat acggttattc tgtttatcac
     7201 tccaataaaa catgtgactc ttcaatagtt cgctatcatt attagaaaag tatcgcgttt
     7261 caacaccatt actaatgata aacatttgta tgtagcggaa taagcctgtg taattttgtt
     7321 tgcggtaacg ttttacttgg ttaaacgcct cattaatatc aatacctcga cgtttcaatt
     7381 caacttggac aaggggtagt ccgttgatta atatcgttac atcataacgt gctttatatg
     7441 tatcctcgac agatacttga ttcgtcactt gaaacttatt tttacaccaa cttttcgtat
     7501 ctaaaaacga caaataaatc tcagactcat catcacgtct aagtggtaat ttatcacgta
     7561 aaatacgggc actctcgaaa atacttttcc catcaatcat cgttaacaga cgttgaaatt
     7621 ctttatctgt taagggattg ccttctaatt tgtccgcatg acgctcattt aaaatcgttc
     7681 taaaattatc aagcaattgc ttattatcac gtatcgttac tctttcgtaa cccaattgtt
     7741 caagttgatt catcatttca ttttctaatg cgtattcact ttggtatgcc attcatatcc
     7801 ccttccatac actttctatt gctctaaata tatcataaac tttaatgaaa aatgtttgtt
     7861 ttttatcttc aaacgtaaat ttattctaat tttattgtct tatcttttaa tatttgtctt
     7921 tgaggtaagt cgtatactaa aatttgaata caaataatca aatcattgat aaattttttg
     7981 tctacgatta atggagggac ttgaatggtg ttaattacct atcaaatcat tttatttttt
     8041 attattagtc taagttacta tttaacttta aatcattaca tggcagtcac tgtaggtaac
     8101 ttcacttcaa tattcggcat gttcgcagcc atactcttta tgtactacta cctactctat
     8161 aaaagtcccg aatacaatca acgcaaacga tttaaacatt tcattcatat cactaatttg
     8221 ataataattg cttttagcac cttcgtatta gttcatttag cattaaaatt attcttcagc
     8281 atttaatttc catctatgaa aaaagcaaag ctcaaatctg aactttgctt taatttgtca
     8341 cgcctttatc attttcaaaa tagcctctat gccagtttta caaacttgta gcaacaattt
     8401 ttcatcaagc aactgaatca catcaaaaac ttcaattgtt tacgttgcaa ctaactttat
     8461 gttagaacgc acacaatatc taatgtttaa aatctatcgt cgcaagatgc ttcctgtaat
     8521 tatcaagtgc cattttcgat tgggttatac aatctagaat cgcatgataa tttaatgcta
     8581 caaatcgata gtacaatata tctaccgtga ataactgtgc aaatagtgac gttgtagccg
     8641 ccatacgcat ttcattttca tcagttctgc cataaatcaa tgcatagtct gcaatttgag
     8701 ccactggatt attagctgta ctagatatag ttatgatggg aatactgtaa tgtgtggcca
     8761 cctgtgcaat tgactgcaat tcactatgac taccttgatt cgtcacaaaa atcatgcaat
     8821 ctctatcatc atgcgtcgca aatgttgaca caagtaaatg cgtttcatgt aataacctga
     8881 catttaagcc aatacgagat aacttttgaa aaagatcacc aatagtcaaa ctcgatgcgc
     8941 caaatccaaa taaaaatatt gtcctggcat ttttcaacac atcacaaatt gcatcaattt
     9001 gcgcatccat aatattagta gctacaaatc gcatcgtatt cgttgctcta gcaatcattt
     9061 tatttttcaa agtttctaca gattcatttt caatcaattc taaatgtgga ttggttgcaa
     9121 tatcttcggg taagtatcga gatatcgcaa tctttagctc ttgaaaacct tgatgtgtca
     9181 ttttccgact aaatctaaca attgatgctg tactaacatt cgtaacatct gccaaatcat
     9241 tcactgtcat atcaatgatt ttatgtggat tctttaaaat gtaatcagcg attatctttt
     9301 ctgtcttcgt aaaatcactc aactgtttat caatgcgata taaaatattt gtcatcatta
     9361 atcacccaac aaatctgtct gtcgcatcgc ctttgtcgtt ccaaataaat atgtacaaac
     9421 gaatccacca gcatatgcag caagtaatcc tgcaatataa cctaaataca tattatctga
     9481 gattaatggt aagagtgaca caccacttgg gcctattgct ttggcaccaa tatgtccaat
     9541 tccacctatt acagcgccac caataccacc accaatacaa gcagttaaga aaggtcgacc
     9601 taatggcaaa gtcacaccat agattaatgg ttctccgata cctaggaaac caactggcaa
     9661 tgcacctttt aaagtattac gtaatgttgt gttgcgttta catcttaccc aaagtgctaa
     9721 tgcggcacct acttgtccag caccagccat cgctgcaatt ggcaataagt aagtagcacc
     9781 agattggtta atcatttcta tatgaattgg cgtaaaaata tgatgaagcc ctaacataac
     9841 taacggtagg aagcttgctc caatgataaa tccactaaat acgccaccaa tactaataat
     9901 tccgttaact actgaaacta aactgtctga aacaaaacct gctaatggca taaagataaa
     9961 gatagttaat agtcctacaa tcaacaatgc aatagtcggc gttacaataa tatcaatcgc
    10021 atttggcaca attttatgca atctcttttc gacaatactt aaaatccaaa cggcaaaaat
    10081 aacgccaata attccacctt gtccaggttg caatggttct ccagtgaaga cattcattaa
    10141 aatattttta ccagcaatac ccgttaataa cgttgtacca ccaatcacgc caccaagtcc
    10201 tggtgttgca ccaaattctt tagccgcatt aataccagta aaaatagcta agtatgctaa
    10261 cataccgtct ttaatgacat tgaatactgt tataagttgc gtaatccaag cacctgaaat
    10321 atagcctgcc accattaagt tactcagtac tgctgcaata ccaccaatta atccagctcc
    10381 aataaatgca ggaatcaacg gtataaagat attggcaatt gatttcaata ctttattcaa
    10441 cttaccattc ttttgttttg ctttatgtgc ttctttattc gcctttgctt tatcagctgc
    10501 atatgattta tagtccattt tttcactatc attgtgatgg tgtggtattg ggtcacctag
    10561 tttaacacca cttaattccg ccatatgatt agccacttta ttgactgtac caggtccaac
    10621 cacaacttga atgcgttcat cgtgtataac acccatgaca ccatcaatat gccttagttc
    10681 ttggtcatct actttatttt catctaatac tttaatacgc acacgtgtca tgcagttcat
    10741 gacactatct atattatcca taccacctac tgcagcaata attcgttctg caagttgttg
    10801 ttctttggtc atttaaatcc ctcctaaggt tgtctatctc tgattgctcg tttaacaatg
    10861 tcaccattgt ttaataaccg tcttgttgct tcttccttag aaatgccaca catacccata
    10921 actgtcgcaa ctttcacatc atgctcagat acctgatata acgccattgc ttcatcatat
    10981 gtgatagcac atatttcttg aataatacgc actgaacggt cgatcagttt ttgattggtt
    11041 gctttaacat caatcatgag gttatcgtaa acttttccga caccaaccat tgtgatggtt
    11101 gaaatcatat ttaaaattaa cttttgtgct gtaccagact ttaaacgtgt tgaaccagtt
    11161 aatacttctg gaccaacttt aacttctact ggatactgcg caatttcact tataactgca
    11221 tgttcattgc atgaaataga tactgttgta gcaccgattg tgttagcaaa tgttaaaccg
    11281 cctataacat atggcgtttt gccactcgcg gcaattccta taacgacatc ttttgatgtt
    11341 aaatctatat ttttcaaatc ttcttccgct aattttttgt gatcttccgc accttctaca
    11401 gccatcgtca tagcatgttg tccaccagca ataataccta taatttcatg agggtcagta
    11461 ttgaatgtag gtacacactc cgctgcatct aagacaccca atcttccact tgtacctgca
    11521 ccgatataga tcaatcgtcc acccttttta tactgtgcaa ttgttttttt aattactttt
    11581 gtcaattgtg gtattgcctt tcgaactgct aacgggactt gctgatcttc tttattcatc
    11641 gtaattaaag cctcttccac agtcatttca tcaagatgca tcgtcgcttc attacgcgct
    11701 tcggtcgtac tattttccat cacttcttac actccctagt tttttgaaaa tcaaatgtat
    11761 cattcggctc gatacaactt aacagtggta agtcttcttt aataatttgt gcaacaacat
    11821 tcacattgtc atgtgcacta agcgtttgtc tcacaatttg catttcacct tgataacgtc
    11881 cgttattcaa attatcaacg gttactgaac caatgcgtcg ttgcgtcgta aactgtggtt
    11941 gaatcgaatg tggacatatt tgtcttgacg tttccgaacg aatgacattt tccggattat
    12001 ccgggcgtac tttatgacac atatcgaaaa ggtaagtcac tgtcgtatca aacacttctt
    12061 caatacataa cgtgaaatgc ctatgcttgc aaaaatctat aagttgtttt gcctgcctca
    12121 tttcaatcaa tgagtctcca actaacactt cagatacacc agtttcttgt aataatttag
    12181 ctgcaacgac aggatgacta tgtctcgttg cttcaattgt tggcaagcct ttatgcaaag
    12241 gacctcgcaa atcactccct acaataaaac catatatttg tgcctttgga ttaaattgat
    12301 aaatgagttc atttttctta ttgaccaagt caacagataa tcccgtatct ggtcttggat
    12361 aatagttatg acaaaatgaa agtaatgtaa aatcattcaa ttgttgatgt aagcttgtta
    12421 acaattcccg ggaaataata cttgcattca aacagcactt taaaccctgt gccattatcg
    12481 cttcgattgc ctcaattgat gtactatgat cgatacgaat cataaattgt gcatcatatt
    12541 gtcgaagatg gtcataaaaa gatggtgtta aaatagatgg attagcatct atgaggtaag
    12601 tcacttgttc atgttttaat aaattgagta gttttgtgaa ataatgatat tttgtctcgt
    12661 catcttcttc tggtatttgt acagatgtaa aaatcatttg gtaaccttgt ttaatcattc
    12721 gcttaatata cgcttcatct aaaggttgtc ctaaatacac tgaaaagcct gtcaaagtag
    12781 ccctccttaa caatataatt attaggaaaa tatagttgag ttgtgtaatc gcttacattt
    12841 tactataaga gaaaacacat tacaatatta atcagttaaa gcctgttcat tgtaataatc
    12901 ttacatattt ctgtcacaag ttaattatta caccatcaaa gattatcctt tcttttaagt
    12961 gctgataata gctgctactg ctggattatt acaacaactt ttatacattt tattcaggat
    13021 tatcttatat tatgttttaa taataatctg tgaacaatta agagatttga aattgaattt
    13081 aataattgta ttgaaaacgc atacttcacc atgctaaaat aggagtcgca aacaaataag
    13141 attcaataag atgtgatggt aagctagata aactaacgta tcagttttat atactactta
    13201 gtcaattgct tcttttacat taattacata caccaacgtg ttactaagta agattaggca
    13261 tgagttaaat taagctgtga tggttaccaa cacagtctat ttgctcgtgt ctttttttat
    13321 tgaatcttaa ataataaata caactttgga ggttggacaa gtgaggaaga aacttttcgg
    13381 tcaattgcaa cgtattggta aagcgctaat gttacctgtt gcgattttac cagcagctgg
    13441 tctgttatta gctatcggta cagctatgca aggtgaatca ttacaacact acttgccgtt
    13501 tatacaaaat ggtggcgtac aaactgtcgc taaattaatg acaggtgctg gtggtatcat
    13561 ttttgataac ttgcctatga ttttcgcatt aggtgtcgca atcggattag ctggcggtga
    13621 tggcgtagca gctatcgcag cattcgtcgg ttacataatc atgaacaaaa caatgggcga
    13681 ctttttacaa gttacaccta agaatattgg tgatccagcg agtggttacg ctagcatttt
    13741 aggtatccca acattacaaa caggtgtgtt cggcggtatt ataatcgggg ccctggcagc
    13801 ttggtgttat aacaagttct ataacattaa cttaccatct tatttaggtt tcttcgctgg
    13861 taagcgtttc gtacctatta tgatggctac aacatcattt attttagcat tcccaatggc
    13921 attaatttgg ccaacgattc aatcaggatt aaatgcattc agtacaggat tattagattc
    13981 aaatactggt gttgccgtat tcttatttgg tttcatcaag cgtttattaa ttccattcgg
    14041 tctacatcac attttccacg caccgttctg gttcgagttt ggttcatgga aaaatgcagc
    14101 tggtgaaatt attcacggtg accaacgtat ctttatcgaa caaattcgtg aaggcgcaca
    14161 tttgacagct ggtaaattca tgcaaggtga attccctgtt atgatgttcg gtttacctgc
    14221 agcagcttta gcaatttatc acacagctaa acctgaaaat aagaaagtag tagcaggttt
    14281 aatgggttct gctgctttaa catcattctt aactggtatt acagaaccat tagaattctc
    14341 attcttattt gtagcaccat tattattctt tattcacgca gtacttgatg gtttatcatt
    14401 cttaacattg tacttattag atcttcatct aggttataca ttctcaggtg gtttcatcga
    14461 ctacttctta ctcggtatac tacctaataa gacacaatgg tggttagtca ttcctgtagg
    14521 tcttgtatac gcagttattt actacttcgt attccgattc ttaattgtaa aattaaaata
    14581 caaaacacca ggtcgtgaag ataaacaatc acaagcggct actgcttcag caactgaatt
    14641 accatatgca gtattagaag ctatgggtgg caaagcaaac attaaacatt tagacgcttg
    14701 tatcacacgt ctacgtgttg aagttaacga caaatctaaa gttgatgttc ctggtttgaa
    14761 agatttaggc gcatctggtg tattagaagt cggcaataat atgcaagcaa tttttggtcc
    14821 taaatctgac caaatcaaac atgaaatgca acagattatg aatggtcaag tagtagaaaa
    14881 ccctactact atggaagacg ataaagacga aactgttgtt gttgcagaag ataaatctgc
    14941 aacaagcgaa ttgagccata tcgttcatgc accattaact ggtgaagtaa cacctttatc
    15001 agaagtgcct gatcaagtgt tcagcgaaaa aatgatgggt gacggtatcg ctattaaacc
    15061 ttcacaaggt gaagttcgtg caccattcaa cggtaaaata caaatgattt tcccaacaaa
    15121 acatgcaatt ggtcttgtat cagatagtgg tttagaacta ttaatccaca tcggtttaga
    15181 cactgttaaa ttaaacggag aaggctttac tttacatgtt gaggaaggtc aagaagttaa
    15241 acaaggtgat ttattaatca actttgattt agactacatc cgcaatcatg caaagagtga
    15301 tattacgcct attatcgtga cacaaggaaa cattacaaac cttgatttta aacaaggtga
    15361 acatggcaac atttcatttg gcgatcaatt atttgaagct aaataatgct tactataaac
    15421 aggtgcgtat accttcataa ggtgacgcgc ctgttttttc tttgctattg tattttgcgg
    15481 catcattgat agttcgctct ccccttaaat tttgaatttt aagatcatca atttaagccc
    15541 cccttcatac tcatttccta aaaagtatta attgttcact attgttagcg ttttcacaac
    15601 aaagtcaact tccttgacct tacactatat tcgaggctat cattttaagt gtaaatatag
    15661 agaaaaggtg gtttttttat gaaacaacgc attggagctt acttaattga cgctattcat
    15721 cgagcaggcg tcgataaaat ttttggtgtt cctggtgatt ttaatctcgc ttttctagac
    15781 gatattatca gtaatcccaa tgtagattgg gtaggaaata caaatgaatt aaacgcaagt
    15841 tacgcagcgg acggttatgc ccgtcttaat ggactcgctg cattagttac tacatttggt
    15901 gttggcgaat taagtgccgt caacggtatc gcaggttcat atgctgaacg catacctgtc
    15961 attgcgatta caggtgcgcc gacacgtgct gttgaacaag ccggtaaata tgtacatcac
    16021 tcacttggtg aaggtacatt tgacgactat cgaaaaatgt ttgcacatat aaccgttgca
    16081 caaggttata tcacacctga aaatgcaaca accgaaatac cacgtttaat taatacagca
    16141 atcgccgaaa gacgcccagt tcatttacat ttaccaatcg atgtcgcaat ctctgaaatt
    16201 gagataccga caccatttga agtgacggca gccaaagata cggatgcatc aacatatata
    16261 gagttattag caactaaact gcatcaagcg aaacaaccta tcatcattac tggacatgaa
    16321 attaacagtt ttcacctcca tcaagaatta gaagattttg taaatcaaac acagatacca
    16381 gtagcacaac tttcattagg aaaaggtgct tttaatgagg aaaatccata ttatatgggt
    16441 atttatgatg ggaaaattgc cgaagataaa atacgagatt atgtggacaa cagcgattta
    16501 attttaaata ttggagccaa attaacagat tcagcaacag caggtttttc ataccaattc
    16561 aatatcgatg atgtcgttat gttaaatcat cacaatatca aaattgacga tgttacaaat
    16621 gatgaaatat ctctaccatc attgttaaaa cagttatcca atatttcata tacgaataac
    16681 gcaacgttcc ctgcgtatca tcgtccaaca tcacccgatt atactgttgg cacagcacca
    16741 ttaacacaac aaacttattt taaaatgatg caaaatttct taaaaccaaa tgatgtcatc
    16801 attgctgatc aaggtacatc attctttggt gcttatgatt tagcattata caaaaacaat
    16861 acttttatag ggcaaccgtt atggggttct atcggctata cattacctgc aacattaggt
    16921 tcacaattag cagacaaaga tcgtcgtaac ttattattaa ttggtgatgg ctcattgcaa
    16981 ctaactgttc aagctatttc aactatgatt agacagcata ttaaaccggt attatttgtg
    17041 attaataatg acggctatac ggtagaacga cttattcacg gcatgtatga accttataat
    17101 gaaattcaca tgtgggatta taaagcttta ccagctgtat ttggtggtaa aaatgttgaa
    17161 attcatgacg ttgaatcatc aaaagattta caagacacgt ttaatgcaat taatggtcat
    17221 cccgatgtga tgcattttgt cgaagtcaaa atgtctgtcg aagacgcacc gaagaaactg
    17281 atcgatatcg ctaaagcttt ttcacaacaa aataaataat ttcatcgtat acagggtata
    17341 agtttaagcg aatactttat taaacgaaaa ggactctgat ataagatgat taattttaat
    17401 aaaaccgctt tagtgttaat cgacctgcaa gaaggtatac ttaagatgga ttatgcgcca
    17461 catacagctg aaaatgtcgt tcaaaacgct aataaattaa tagatgtttt tagaaaaaac
    17521 aatggcttta tcgcttttgt tcgcgtgaat ttctatgatg gtaaagatgt attgcaacca
    17581 aatgcaatga tctcattacc accaaaagaa ggcgacgact acagtcgttt ccatcattta
    17641 ttagacaaga gagatgacga ttttgtcata gacaaacgac attttagtgc atttgtagga
    17701 acagatttgg acttacaatt gcgacgtcga ggaattgata cgattgttct tggtggtgtc
    17761 gcaacgcata ttggcgtaga tacgacagcg cgagatgcct atcaattaaa ctacaatcag
    17821 ttttttgtta cagatatgat gagtgcacaa aacgaaacgc tacatcaatt tccaatagat
    17881 aatgtattcc cattgatggg acaaacaata actacaaacg actttctaaa tgtattgaac
    17941 taaacatata cttcccccct tcgatcatgt tgagggggat ctttatttca caaagtatta
    18001 atacgtcggg ttgtctaacc ttctatattt aacatattct atatctgtta aatcgttctt
    18061 aacttacgcc cctactacat aaaaaacagt attcattccg gaattttcaa aaaatttagt
    18121 atttattgca aaattatgta tcactttatg tttaattttt gatattatct taattaagta
    18181 gatttttata agttctaaaa aggagaacaa atacatatat gaagaagaaa ctaacattta
    18241 aagaaaacat gtttataggt tctatgttat ttggtttatt ctttggtgcc ggcaatctta
    18301 tcttcccaat acacttgggt caagctgctg gttctaacgt ttttatcgct aacttaggat
    18361 ttttaattac agcaattggc ttaccatttc taggtatcat tgctattggc atttcaaaga
    18421 catctggttt atttgaaatt gcatcgcgtg ttaataaaac atatgcttac attttcacga
    18481 ttgccttata tctagttatc ggaccatttt tcgccttacc tagactggca acgacatcat
    18541 ttgaaattgc attttcgcca tttttatcac caaagcaaat cactttatat ttatttattt
    18601 ttagcttcgt cttctttgtg attgcatggt tttttgcgag aaagccatca agaattttag
    18661 aatatatcgg taaattttta aatccggtat tcttagtatt attagcaatt attttattat
    18721 ttgcttttat ccatccatta ggtggcatat ctgatgcacc tattagtaaa caatatcaat
    18781 cacatgcctt atttaacggc tttttagatg gatacaatac cttagatgcg ctagcgtcat
    18841 tggcatttgg tattatcatt gttgcaacga ttaaaaagtt aggtatcgaa aatccaactg
    18901 atatcgctaa agaaacaatt aagtctggta ctatcagtat cattatgatg gggatcattt
    18961 ataccctact agcaatcatg ggtacattaa gtattggtca tttcaaactt agtgaaaatg
    19021 gtggtattgc cttagcgcaa attactcaat actacttagg taactacggt atcgtcctgt
    19081 tgtcacttat cgttatggtt gcttgtttaa aaacagccat cggtttaatt acggcatttt
    19141 cagaaacatt tgagcattta ttcccgaaac tgaattacct agcgattgca acagttgtaa
    19201 gctttatttc gttcttattc gcgaatgttg gtttaactaa gattattatg tactcagtcc
    19261 cagtgttaat gttcttatat ccattagcaa ttgccttgat tgtactaaca ttatttagta
    19321 gcaaattcca tcattcaaaa cttatttatc aatgtaccat tttctttaca atgattgctg
    19381 cattagtaga tggattaaaa gctagtccag agttcatttc aagcacatca ttctcacaaa
    19441 ctttgattaa tttcagccaa aaatatttac cattatcaga cattggtatg ggctgggttg
    19501 ttctcagttt gattggtttc attatcggct tcattattta taaaattaag catcgtaaaa
    19561 ttccacaagc ataatactat gccacagtca tatgttaaac atatgcttgt ggcatttttt
    19621 attcatacta cattaaactg caatcgtata catacatatc aatgattatc cacaaaaaat
    19681 attagtactt tcattttaca aatcacatta atacaaacac aaccttatct ttatattatt
    19741 aaatttacat ttgacactta tattgaacaa ctgtaatata ttaatattaa ttctttaaat
    19801 tgtataaata taaaggaggg agaccgatga attcaatcat tgaattaact gattattata
    19861 gctctaataa ttatgcacca cttaagcttg tcatttctaa aggtaaaggt gtcaaagttt
    19921 gggatactga tggcaaacaa tatatagatt gcatttcggg tttttcagtt gcaaaccaag
    19981 gccattgtca tccaacaatt gttaaagcga tgacagaaca agcttcaaag ttgtctatca
    20041 tttcacgtgt cctttatagt gacaatctcg ggaaatggga agaaaaaatt tgtcatcttg
    20101 ctaagaaaga caaagtactc ccccttaact ctggtactga agctgttgaa gcagccatta
    20161 aaattgctag aaaatggggc tctgaagtta aaggcattac tgacggacaa gttgaaatca
    20221 tcgctatgaa taacaatttt cacggtcgta cacttggctc attatcacta tctaaccacg
    20281 acgcatataa agcaggattt caccccctac ttcaaggcac tacaactgta gattttggag
    20341 acattgaaca attaacacaa gctatttcac caaatacagc agcaattatt ttggaaccaa
    20401 ttcaaggtga aggtggcgtt aatataccac cgaaaggata tattcaagct gtgcgtcatc
    20461 tatgtgataa atatcaaata ttattgattg cagatgaaat tcaagttggt cttggtagaa
    20521 ctgggaaatg gtttgcgatg gaatgggagc aagttgttcc agacatttat attttaggta
    20581 aggcattggg tggcggctta taccctgtat ctgctgtact tgcaaataat gatgtcatgc
    20641 gtgttctaac accaggtaca catggttcga catttggtgg taacccttta gccattgcaa
    20701 tatcgacggc agcgcttgat gtacttaaag atgaacaact ggttgaacga tcagaacgct
    20761 taggttcatt tttattaaaa gcgttgctac aacttaaaca tcctagtatt aaagaaatta
    20821 gaggtcgtgg tttatttata ggcatagagc ttaacacaga tgctgcacct tttgtggatc
    20881 aactgattca acgtggaatc ttgtgcaaag acacgcatcg tactatcatt cgattgtcac
    20941 cacctctagt cattgataaa gaggaaatcc atcaaattgt tgcagctttt caagacgttt
    21001 ttaaaaatta acaattaatc atttatatat gatataggag ggattcatga tgattaaagt
    21061 aggtatcgtt ggcggtagcg gttatggcgc aattgaatta attcgattgt tacaaacaca
    21121 tcctcatgta acgattgcac acatctactc acattcaaaa gtagatgaac cgttgaaatt
    21181 aacatttcca catttacaac atattatgca acatttcgaa gcacttacag tggacaataa
    21241 tgactgtgat gtgattttct ttgcgacacc agcacccgta agtaaaacat gtatccctcc
    21301 cttagtagaa aaaggtattc atgttatcga tttatctggc gcatttagaa ttaagaatcg
    21361 tgaaatatat gaagcatatt acaaagaaac tgctgcagca caagatgatt tgaatcatgc
    21421 tatttacagc atttcagaat ggcaatcgtt tgataacaat ggaacgaagc tcatttctaa
    21481 tcctggctgt ttccctacag caacattatt agcattacat ccacttatta gcgaaaaaat
    21541 agtagatttg tcatctatta ttattgatgc taagaccggc gtgtcaggtg ctggtcgttc
    21601 attatcacaa cgtgttcatt tttcagaaat gaatgaaaat ctaagcgctt atgcaatcgg
    21661 aaaccataaa cacaaaccgg aaatcgagca atatttatct atcattgcgg gtcaagatgt
    21721 atcagtcata tttacaccac atctcgtacc aatgacacga ggtattttat caacaattta
    21781 tgtcaaattt tcatctgaat atacgactga atcattacat aaattaatga cctcttatta
    21841 tgctaatcag ccatttgtca gaattagaga tattgggact tttccaacca caaaagaagt
    21901 actcggtagt aactactgcg atatcggtat ctatgtagat gaaacatcac aaacagcaat
    21961 tttagtatca gtgattgata accttgtcaa aggcgcaagt gggcaagcca ttcaaaattt
    22021 aaatatatta tatgattttg aagtgacgac tggcctaaat caatcaccag tttatccata
    22081 aggggtgtta gaatgaaaca tcaagaaacg acatcacaac aatataactt ttcaattatt
    22141 aaacatggcg atattagtac acctcaaggc ttcacggctg gtggtatgca catcggttta
    22201 cgcgctaaca aaaaagactt tgggtggatt tactcatcgt ctttggcaag tgcagctgcc
    22261 gtatatactt taaatcagtt taaagctgca ccacttattg tcactgaaga cactttacaa
    22321 aagtctaaag gaaaattaca agcacttgtt gttaattcag ctaatgcaaa ttcttgtacc
    22381 ggtcaacaag gtatcgatga tgcacgacaa acacaaacat gggttgctca acaacttcaa
    22441 ataccatctg agcatgttgc tgttgcttca actggggtca ttggtgaata tttgcctatg
    22501 gataaaatta agactgggac cgaacatatt aaggatgcta attttgcaac gccaggtgcg
    22561 tttaacgagg caattttaac aactgatacc tgtacaaaac atatcgctgt atcactaaaa
    22621 atcgatggta aaaccgttac aattggtggt agcgccaaag gttcaggtat gattcaccca
    22681 aatatggcta ccatgcttgc ttttataaca accgatgcat cgattgaatc gaatacactt
    22741 catcaattat taaaatcttc gactgaccat acatttaata tgattactgt tgatggcgat
    22801 acaagtacaa atgacatggt attagtcatg gcaaatcacc aagttgaaca ccaaatactt
    22861 aatcaagacc atccacaatg ggaaacattt gttgatgcat tcaattttgt ctgtacattt
    22921 ttagctaaag ctatagccag agatggcgaa ggcgcaacaa agttaatcag tgtcaatgtt
    22981 tcaggcgcta agtcaatatc agacgcaaga aaaatcggta aaactatcgt aagttcaaat
    23041 ctagtaaaat cagctatttt tggcgaagat gccaattttg gtcgaatcat tacagctatt
    23101 ggctatagcg gatgtgaaat tgatcctaaa tgcacatatg ttcaactgaa ccaaatacct
    23161 gtcgttgata aaggtatggc tgtactattt gatgagcaag ctatgtcgaa cacattaact
    23221 catgaacatg tcacaattga cgttcaactt ggtttaggta acgctgcagc gactgcatac
    23281 ggttgtgatt tatcctatga ttatgtgcgt atcaacgcat catatcgaac ataaggtggt
    23341 gttggttaga tgaaatttat tgtcattaaa attggtggca gtacacttag tgacatgcat
    23401 ccatcaatta ttaacaatat taagcattta cgattaaaca acatctaccc tattatcgtt
    23461 catggcggtg gcccatttat taatgatgca ttatcaaacc agcaaatcga gccacacttt
    23521 gttaatggcc taagagtgac tgataaagca accatgacca ttactaaaca cacgctcatt
    23581 gcagacgtta acactgcatt agtagctcaa tttaaccagc accaatgttc tgcaataggc
    23641 ttatgtggtt tggatgcaca gctgtttgaa attacatctt ttgatcaaca atatggatat
    23701 gtcggtgttc cgaccgcttt aaataaggat gctttacagt atttatgtac taaatttgta
    23761 cctatcatca attcgattgg tttcaataac catgatggag aattttacaa tattaatgct
    23821 gacacgcttg cctattttat tgcatcatca ttaaaagcgc ctatttatgt attaagtaat
    23881 attgcaggtg tactcatcaa tgatgttgtt atacctcaat tgccattagt cgatattcat
    23941 caatatattg aacatggtga tatttatgga ggtatgattc ccaaagtgct agatgccaaa
    24001 aatgcgattg aaaatggctg tcctaaagtt atcattgcat caggaaacaa gccaaatatc
    24061 attgaatcta tttacaataa tgattttgtt ggcacaacaa tccttaattc ataactatga
    24121 aattaagacc taacaagttt tgacacgcga tgattccagt tcgattatcc attgcgctaa
    24181 aacatttatt tgccgttcat ctcgttaaca attttgaata cagtacgata caatatgaga
    24241 tgtaaaaaac taataacctt ttacaaattt gtttatcaaa atattttaag ttttgcaaag
    24301 ctttttattg tgattatttt cacaaaatac tataatgagg atagtaaata gagaggagtc
    24361 cttaagttga cgaaacgaca aatgggtata ttcatttatg ctggaattat cggtggcttg
    24421 ttatctggaa ttgtaaaatt aggttgggag gtcatgtttc cacctcgcac accagaacgt
    24481 aatgcaacga acccacctca agagttattg caacaattag gatttagtag tgagtttacg
    24541 catcaaacat atacattttc aaatatggaa ttgccttggg taagctttat tgtccacttt
    24601 agtttttcta tcgtcattgc aattatttac tgcatattag ttaaaaaata cgcttactta
    24661 gcaatgggac aaggtgctgt ttttggtatt gctatttggg tattattcca ccttatcatt
    24721 atgccaatca tgcatactgt acctgctgtg tgggatcaac cattccaaga gcatctgtca
    24781 gaattctttg gccatatcgt ctggatgatg acaattgaat tagtgcgaca acattttgtc
    24841 tatcgctata aattaaatta atacactgac taacattaac gtgagtttta aatcatcgtt
    24901 tgagtatgat gattgatgct cacgttattt tattaactga catgatatga ttccagccaa
    24961 cttacgtgag cattaaagtc tcaaatgcgt cgtaacaaac tattattttt ggcaatttca
    25021 atattgctca gtatattttc accttatcac ttactttaat ctcgtcatga ttttgaatga
    25081 tgccatcgtg tattcacctt tcatttttcc aataaaaaaa catctaacag taaacattta
    25141 ggcagtatag tttaaaatca ctgcgcaatg atactgtcag acgtcatatt aactactcaa
    25201 taactgaaat acagacactt ttttataacc ccagggtgcc tgtcctaaga aacatacctg
    25261 tagcataaac tgatcaaaaa taaattgttt gaacttcact tcacgtgatt gataaaagtg
    25321 tgattgtgtc atatcataaa tgtccaatcc tttgattaaa ccttcaccaa tcaattttgt
    25381 aaaactttct ttttgtgtcc atatttgata aaaatcattt aaactacata tttgatgtgc
    25441 ttcgtttgta gagaaacacg tcactaacgt acgccagtct aaacgttgtg atatcttttc
    25501 gatatctata ccaactggtt ctttatcgac aacacacacg atataaggat aactatatga
    25561 taagctcaca tagatgggct gtccatcacg attgtgttga acaatatctg ccttacctcg
    25621 tggcgaaatg tgataatgcc attcatgtgg taataaacct gtgtcatgtt gaattccata
    25681 ttgcactaaa atatctccca atctgtgcat gagtttatct tgattgtatc tatagttgac
    25741 tgtacgcggt tttttatatg accaacgact ttgtgatatt aattcttcaa tacttttcaa
    25801 gttactctgt aattgcatta caaatactgt cataactttc cctacttact tattgaatat
    25861 tgttttgata tattgtgccc aatgatacag ccaattgtta gttatcgttg gccatttttc
    25921 actgatgtga ttcattattt ttaatgttaa tgttgtatct atcattgcta gttgttgttc
    25981 acggtcaaca ctagttaatc caatcgtttc gtacatgtct tgtttctgta aaatttcatt
    26041 aaatgattca tcgctgacga gttcaatttc tttgcgctta acgcattcta acaaagattt
    26101 caccggcatt ttattaggtg atagcacatg gtaaatgatt tgtggtgtgt tgacttgtgc
    26161 taatgcgaca atttgtcttg cagtcgtatc cacaaaagaa aaatctacag gcatttcagc
    26221 catgctaacc ccgatacaat ccagttgtaa caaatcattc attaccattg aaaaacggtt
    26281 agtctttata tttctcatat gccatcttcc attgtaagga ctcgtcaaat taccaacacg
    26341 tacaatccga ccatctaagc cattatttac agcttctaat acttttaatt cactataaaa
    26401 tttgctccgt gtatatggtg atgttagtag ttgcccttta tagacatccg cttctgaaaa
    26461 tgtcacatct tctgtgtcta tatcaaaata agttcccaca cttatcgtag acacatatat
    26521 taaccttgca tgatgttgtt gtgccaaacg tatgacatca acagtacctt gaacatttac
    26581 tttttcaaat tcatcatcat caccaaagtg atctgtacga gcacctgcat gaataatcgt
    26641 atccatgttt tctggtaaaa caacatcatc catacactca aaatcaccaa caatgacttc
    26701 aatgtttgat aacatcattt caaccgtctc ttctgaaaaa taatcattta aattcgtcat
    26761 caacttatac catgctattt cctcattatc agcacgtatg aaacaataaa tgcgatgact
    26821 gtatccttgt agtgcttcaa tcagataagc acctaaaaac cctgtagcgc cagtcaatag
    26881 tgtatttcct agaggtcgat gacttagact atcctctaaa acacccaaat tataacgaga
    26941 cataacaatc ttttgtaatt ccgaaagatt atccggtaat gcaactaatg attgttgatt
    27001 ttggtgcata taattaacaa tctgtcgcac ggttttatat tggtataatg tctgcattga
    27061 aatatgatgg ccaaatcgtt ttaaatgcga gacaactaac atcgcctcta atgagttacc
    27121 accaagttca aagaaatcat cgtcaacacc gacatcattt tgtttcaata cctctccgaa
    27181 tacatcaaca aatgtctgct caatttcatt agatggttcg ctatacactt tattagactg
    27241 ttgtataggt gatggatttg gcaaacgcgt agtatccacc ttgtcattcg tggttaatgg
    27301 catacaatcg atatgcgtta tagtcttagg aatcatatac ttaggcagat gatcatttaa
    27361 atattgcttc aaatcctgct ccatttgttg ctctccgaca taataagcat tcaatatatc
    27421 atgcgtatca aagtgactta ctgttacaac acaatcagat ataccacgaa tagctaatat
    27481 tgcattttca atttcatcaa gttcaatacg gtacccgtta actttcactt gtttatctat
    27541 tcttcctaaa aattcaattt gaccatcaga tgtataacgt gctaaatcac cactatgata
    27601 caactttcct ttaccaaatg gattattttg ccatttatca gccattaatt ctggacggtt
    27661 aatatatcct atcgctaaac tatcacctgc aatacacaac tcgcctggca taccaatacc
    27721 gcataacaaa ccatctgaca taatatacac ttggatgtta gataagggtt tgccaattgg
    27781 aatcgtctca ggtatcaaat caccacaatg atgtgaccaa tacgatgtga tgactgttga
    27841 ctcagatggt ccataggcat tgaaatacgt gccacaatgc ttctcaatat atttaacaaa
    27901 ggatgccgta ctagttgccc cgcctgtaat caacttttca atataaaagt cttccataac
    27961 actacacatc tgtaacggaa tcgacgccac cgtcacacga tgcttattaa tgagttgttg
    28021 taactgtgct ggattaacac gttcctctct atctggaatc acaagcgtat gaccatttaa
    28081 caaacaacaa taaatctcca taactgatgc atcaaaaaca atatttgcat gttgcaaaaa
    28141 tacttcattg tcgcctaatt gcaattcagt tgaccatgca tgcactaaat tcaacaaatt
    28201 tcgttgtcgt atggcaaccc ctttaggcat cccggtcgta ccagatgtgt aaatagcata
    28261 catctcatta tctaacatcg ctgtgttttc aagttgattg ccatgtaaat catcatattg
    28321 ttcattttcc tttgattcaa caaagccttt agcattttcc aattgaatga ctggtaatgt
    28381 cgtttcaatt tcaacgccgt aagacatgac tgcagttact ttagcatcct ccaaaattgc
    28441 accttgtcgc tttttcggaa aatcaatatc gataggtata taagatgcac ctactttaac
    28501 tgtcgccaac atcgccgcaa tcatttcaaa actacgttct gtaaacaagg caacccgttg
    28561 accattgccc acaccatttg ataggagcat gtgcgcaatg gcatccacat agttgcgtaa
    28621 tgtttcatac gtcattgtca aatcattcat gactagcgca acatgattac cttgtcgtga
    28681 gacaacttca ttaaagtaac ttatgataga tttatttccc gggacattaa gcattcgatc
    28741 gttaacatgc gtattgaccc aatttagaag ttcctccgtg ccgtttggta tatcacaaat
    28801 ttgtagtgta tcttgatgct tcaaaatata atcaatcata atcatacatt gattacccat
    28861 gtgacgaact gtttctgagt gatataaatc ggtattatac tcgatattga ttgtataatc
    28921 atcgcgatct tcttcaatga tgaaagataa atcaaatttc gccgtcactg atttgggttg
    28981 aatgtgtgtt aatttactat gcccaaaatg agcatgattc gtttcattgt tttgtagtac
    29041 taacatgaca tcaaataatg gattccgtga ggcatcatgt gattgatcta agtcatttac
    29101 taaacattcg aatgggtatt cttgatgctc gtatgcctcc aaactcattt ccttaacctc
    29161 ttgtaaaaac tgtgtccaca ttttatcagg tgacggttgc cctctatata ccaacgtatt
    29221 agcaaacatg cctagcattt gctccgtgcc tttatgcata cgcgcactca tcacactacc
    29281 gacaacaaca tcatcttttc gagcatatct gcttaacaac gtcatgacca cactcataaa
    29341 gaacataaaa tcagtaattt gatgcttttc tacatacttt tgaagtagct gtctcatttg
    29401 ttgattcatt gtaaatgaca tcattgctcc atttgtcgtt ttaatatttg gtctaacata
    29461 gtctgtcggt aagcttaaaa taggtacttc atctttgaat tgagataacc aatattgtct
    29521 atgtttcgtc atatcacgat gcgacatcca ctcactatag tctttatatt gcaatttaag
    29581 tggtaacaat aatttatgtt gataaagtgc gttaagatca ttcattaatt gtatattact
    29641 cataccgtca ttaatgatat gatgtgtatc tataaacagg tatgcatgta agggacttct
    29701 aatgtatctc actctaattt gacttggctt ttccaaatta aaaggtgcca caaattggcg
    29761 catgatttct tgttcatccg taaaatgcgt gttaacttct tcaaagtcag ctacaacatc
    29821 tgccacaata cgttgtcgaa cctcatcatc tacaacaata tattgtgttc gtagaatctc
    29881 atgtcgcgct atcaaatgct gaactgcttg ttgcaattga gctacattaa gtcctgatga
    29941 taaccgccat aaaaaaggta cgttatacac cgtatcttta tggtttgatt tccataataa
    30001 atacatacgc ttttgtgcag agctcagcac ataatcatct ttaactatag cttctggaat
    30061 cacttcatag ttttgttctt gaaccttagc aatcgcttgt gctagttcaa atacagttgg
    30121 cttttgtaat aaatcaccaa tttgtaatcg tttcccagta gatgcctcta tccgattcac
    30181 cactaacgtt gcttttaatg aatggccacc taattcaaag aaattatcat gaatacctac
    30241 ttgattcaca tgtaaaatat ctgcaaaaat ttggcatagc aagtgttcgg tatctgtact
    30301 cggtgctaca taggcatccg tatcgacata gtccatgata ggcaatgcct tcttatctaa
    30361 tttcccatta atagtaatag gaatttgctc aatatgcatg aaattaactg gtatcatgta
    30421 ctccggtaag gtcatacgta attgtgattt aatcttatta tgtgataatg tatgcatcgc
    30481 ttcataataa gcaacgatat actgatcttg atcatgattt tgaacaataa caactgcttt
    30541 attaatacct tgtatacgct cgagcgcatg ctcaacctct gacaactcaa tcctaaaccc
    30601 tcgaatctta acttgtttgt cctttcgata taaataatct atgttgccat cgggtaacaa
    30661 acgaacgata tcaccacttc tatacatcag ctgatttata tttgaatctt tgataaattt
    30721 atctgctgtt aattctggct gatttaaata acctgcagct aacccaaagc cacttgtaca
    30781 taattctcca ggaataccaa caccacatcg acgctcgcct tgcatgatat aaacatgagt
    30841 accaagaatt ggtttaccaa taggaatacg atttggaact ttgttaggta tattatacgt
    30901 cgttgtaaat gttgtatttt cagttggtcc ataaccatta ataatttgag gatgcttcgg
    30961 tttttgatta agcaaatcca cccacttagc attcaatact tctccaccaa ttaataaata
    31021 ctttaacggt accaatactt ctattcgttc actagcaatc tgattaaata atgaggaggt
    31081 taaccacata gtattaacgt cattttcatt gattaattgt tctaacgcta ttggatttaa
    31141 taattgttct tttttagcaa caatcagctt tccaccattg agcaatgcac catatatttc
    31201 aaatgttgca gcatcaaagg ctatagttcc tgataacaaa atcgtcgtct cttcatttaa
    31261 tggtacataa tgattttgat gaaccaagcg aacaatacct cggtgcggaa ttagtgtccc
    31321 tttagggtta ccagttgtcc ccgacgtgta aataacataa gcatgatctt ctaacgtgtt
    31381 acatttagaa agattatcaa tatttttcca cgctatctta ttcaaatcaa tgtgattaat
    31441 attttgttta ccattttcat ataaagcttg gtacgttatt acaactttag gcgttgcatc
    31501 ttttaaaatg tactcctgac gatcacttgg atagttcgga tcaattggca cgtaagcccc
    31561 accagctttc aacacaccta tcatcgctat tatcatctca atactttttt cagctatgac
    31621 agcgacacga tcattaggtt caacaccata ctggtttctc aaacggtgtg ctaaatcatt
    31681 cgcgcgtgca ttcaatgttt gatatgttat aaacactccg tcaaattgca cagcgacatg
    31741 attcggcgtt gcttcaactt gttgctcaaa taaggtaaca actgtttgcg catcatctat
    31801 ctcaggcaaa cttacattga tatcgtcata taattgaata tcacgttctg tcatcaaatt
    31861 aagttcatct acagttgttc gtttatttcc attttcttca gtaatttgca aataaatatt
    31921 tcgaactaag tcactcagcg tctcgattga gagcaaatca tatgctgcac tgttatatat
    31981 aattttgaac ccgtgttgat gtggaaaaat ttcaatatcc gctaaaaatg tatgtgcatc
    32041 atgtatttga tgtacatcct caataacatc attacaacaa gacatcatat gatgataaca
    32101 atgaaaaata gtctctagtg aaagcgaaga cttcgcgcac tgtaattgcg acatattttg
    32161 caacacacat ttattaaaat ctgttgtaaa acgttgacat acatcttttg catcgattgt
    32221 taacgttaac ggcacaatat ttccgtgtaa atcatttggt aaatgtgatg gtacatgtat
    32281 acctaatgtg acatcatgtt gttgactcat tatatgatta gctaaataca cactaacagc
    32341 caacgatgcc atatctattg atgtcatatc atcaatcaaa tacgtttgat ataaagcttg
    32401 ctcaaatgga tgcttaattg gtaaataact atcaatatgg atgtcagagt tattctctaa
    32461 ccgaaaatag tttgagtcta atgctatatg cgatgcatcg tgattgtctt tatcatcatt
    32521 tctatttata tgtgcatgct gtcgagtatt gttaataaca gtattgccac gatatgcatt
    32581 gcataaatca tcaagaaaaa tatcaatttg actatcatcg aaaatggaca catgaaaatc
    32641 taatagtata tatgcagcat cagcgaactg aaacaattta aatttgaata aaggtgaatc
    32701 attaaaatgg taagtactta attcttgctt aaaaaaagct tctaaatcat agtttgcgga
    32761 agaagatgga acttgtttta tctcaataaa aggcagaaat tcatgaagta tcattgttaa
    32821 attgtcatcg gtagtaacat caaaaaaatg tcttatagat gcatgttgtt gcacaattgt
    32881 cgataatgca tacatcattt tagtagcttc aacattttta gcgagtttaa cccaatacgc
    32941 attacggtgt gtcgttgatt ctgtattatt tttgtatata cgaaaatatt cctgctgaaa
    33001 tctcaaatta cccataatca taaaaagtcc ttctttcata tcataatact cattacttac
    33061 tgaaattgca tgatgatatg ataaccgacg aaatgttaat taactcgtta tgtaatgatt
    33121 aatataaaac accattcgca acatatgagc gatatattct accctaaaat acatcttgta
    33181 tcatcgttac aattggtata tttttcaatg taaattacat acatcttcga taaatagcac
    33241 actacaactc gttaatcact ttctgttgtt cacatctcat tgcaaattca atattgttgt
    33301 tacaaaatat ccatgaagca agtttatatt aaacaaacaa ctcgcataaa acaattgtta
    33361 tctttaaatt ttaacaaatt cttaataaat ttatctctat tttaattacg accaaattaa
    33421 taggttttca atataaaaag atgcataaaa taaatattta aataaattca atttgtattc
    33481 acttgttttt gtcccccgaa tacaccagca acaagcatgc tagcaccaat tgttaaaacg
    33541 ataaacatat acagtcccat ttgtaatgac gttaagaaag cacccaacac aatccctaac
    33601 ctagctagtg tttctgaaaa atgaatacct aatgcattaa ctgcactata tgttcctctt
    33661 ttagctttag gaataatttt aaagcgttgt tctgaaacta taggcgaata aataatttca
    33721 cctacagtcg caattatcat aaaaacaact aataagccaa actgattaag ataggttaga
    33781 ccactatagc caacaatata aatcagcaaa ccagtgatta aagctttttt aaaatctatt
    33841 ttcaatacaa ctttcgagat tgaatacgtg agtaaaatga cgacgaccgt attaatcatt
    33901 agcaagattg ctaacatctt agcacctgta atatcatatg aacctatact tattgtttca
    33961 aactgatcct ttagtctaat agcaatatat gaggagattg aaaattcacc catcatgatg
    34021 atactgaacc ccgaaatcaa taacatataa ttacgctctt tcaaaactaa tttataactg
    34081 cgaaatatat tcattatttg taatttttga taacgacttg catgcctctt gtcatcactt
    34141 tgctttactt gatttcggtc ttgaggtaac caaatatata aaataaagag tacaattaaa
    34201 aatatacaag ctgctattaa gaaaagtagt aacatactgt agccatacat caagccacct
    34261 aacaatgccc caatagctac cgataagttc gtcatccaat agctaatctt gtaaatataa
    34321 tgttccacgt cttcggtaat tgcatccata attaatgtgt ccataactgg aaattgtaat
    34381 ccccaaacga ttgtaaatat ggcatatgca acacaaaaac caataatttg ccacaattga
    34441 tgtgacccaa atatgcccat gaacacaagc attatcacca tcgtcgcttg ataaataagt
    34501 actagcaact ttttcggaaa tatctcaata aggtaaccag atataatgga caatggaaat
    34561 tttagaacca ctaaaccaac aagatatata ccgacaattg attgacttaa catatctgtt
    34621 aaatatagtg ctataaacgg tataaatgct gtcgtaataa ttagctgtaa aaaattgcta
    34681 atcaatcgta ctttcaaagt cgtacttaag cgtttcatta ataactacac ccctatccac
    34741 atatatacac actaccattt atataaaatc tattattaga ttatacgttg aaacaaagaa
    34801 tttttagtta tacaaagtta tttaacaaac aaatgcatcg attttacaat gcatcttgca
    34861 tataatgcct caaaaaaatt agcactatat gactatatat tattccaaat gaatgaaatt
    34921 tatcaataat tgcgtaagtg aacacatatg tgcgtaaatg ttaaatcatt gtcctaacat
    34981 agccgtgact atacttagat gtaatcataa aaaaactact gccatcttta caacagcagt
    35041 agtgcttata tgattcattt atataaaatt gaaagcgtta atcgctagcc cagcatcctt
    35101 attctattta gctgtataac ttttactagc aatacgacca cttgcaacaa taatatcttt
    35161 atcttggaaa ggttcatgat tgaagaaacg ctctaaaata tctttaactc catcttcaat
    35221 acgtttttgt gcttctaaag tcatacctga atagtgaacc gtcatagcat ttctaggcat
    35281 tgttctccat ggatgatcag caggtgcagg ttgtggatac caaacatcac cagcatatcc
    35341 ttgtaaatgc tcggatgcta acgcttcaac taacgcatcg cgatttacaa ttttaccacg
    35401 tgcagtattc actaaataac tgtgtttttt catacgactt aaaacatctt tatcaaataa
    35461 gttatcagtt tctggtgtta atggtgcatg aattgtaatc gcatcacttg ttgaaacaag
    35521 ttcatcaaag cttacaaatt tagacaattt atggtcttgt tgattgattg gatcatagtg
    35581 ttgtaatgtt acattaaatg gcgctaatct ttcagcaaca agttgtccaa ttcgaccaaa
    35641 tccaaaaata ccaattgttt tgtgttgtaa ttcatgcgca tgattaccta cttgagacaa
    35701 gttccattca ccttctactg attgacgatg accttcttca tagtttctga gaagtattaa
    35761 taaatccata accgcatgtt ctgccacact aactgtatta cttcctgtaa cttcaacgac
    35821 accaatattg tgttcacttg ctgccgctaa atctacatgg tcagatccta cacctgctgt
    35881 aattgctaat ttcaagttcg gtgctttttc aatacgttca cgagtcatat atgcaggata
    35941 aaatggcgca ctaataatca catccatatc tggtaaatgt ttatctaagt ctttaccatt
    36001 atctgctaat ataatgaact catgtcctct ttcctctaaa aatgttttta atcctaatgc
    36061 ttttttagta ttaagtaatt gattttcttg accttctact gcttctggaa ataacgctac
    36121 gattttcata tccatctctc ctaaatgata aattttgact tccttaaagt acgtgttgca
    36181 tgttgcttgt tttaaaaata tgacaaaaaa aacggcaccg tttgacatac cacccccttg
    36241 aattattgtt aaattcaaag cgaagttagc aaacagcacc gtgtacctta caccagcaac
    36301 acttattcta ttgacttatc gaaaacaaat attgaaatga cttcatcatc attcaaatca
    36361 atatctgtaa ataattttac aaacttagcg cctacaagac tttccatctc atttacatct
    36421 atttctttat acatctgttt aatcttctct gtgcgtgtat aatggagcat cgattcattg
    36481 cgtttgtcgt ttaggtaaaa actctcaact ttactcaaaa cacctgtcat atgtgcaatc
    36541 gcccaattat ctttaaacga cactcgaata ctattgggtc ctttacccac gtattcttta
    36601 cgataagatc ttactagatt cgagaattgt tgttctttaa tatgcgtatc tatagtattc
    36661 accttcttta aaataagaag ttcattaatt gttcacattt tcattataca ccttattaac
    36721 actttttcaa tgataacgat tattattatt acgaaatgta tattttccta gttaaaagga
    36781 tataacaaat aaacagaaaa tgtttataaa ctaagattta tattatttta aaaaatgttt
    36841 caacaaacgc atctaaacaa caaatcagca tatcatactt accccttcaa ctctgcctca
    36901 agtttaccta aatgtcttaa tgttggtgtc aatgctgcga agaagaatcc ttcttttaac
    36961 ttgcgaactt gtggcgaata tggtgtgtac gctcttgaac caccattgac aacagaagct
    37021 tgatttacat ctaacaataa atagccaatg tccttcttca atgatattaa ttcacttaaa
    37081 tgtgaagtta agttaccgtc atctaatatt gcataatatt cctctctaag ttgacgataa
    37141 cgttttttaa aagcttcaac atcatactct aaatattgat ttattccgtt ttgcgcattt
    37201 gaaaatgcat caattaactc taaagaactt ttaattgagc ctaatcctat tggaatttgg
    37261 taagcaataa attgcgggcg aatagttgcc gcaaactgct tcgcatcatg cgtgataatt
    37321 tgtgattgtg gcactacgac ttgattcaat gtgatttgat acgttgctga cccgttgact
    37381 cctaaaaaat ttgttttttc aacaagagtg ataccatctt gattggcacg tagaatgaac
    37441 atgacaaatt catctgatga ttcatgtttc gaaattgcac caaaataatg gtccgcttga
    37501 atattactta cagctggcat acgtccactg acaaccaatt gtccatcaac ataagtatgt
    37561 tcaaggttta atttttctaa atcattaaat gacttcatcg gattagacaa tccggtagca
    37621 cctaatattt ctccagataa caattgctgt tgtaagtcat tatttaaatg tggctgcgtg
    37681 gcattttcta aatacgttga aaaagctaat tggcaccata aacaaaatcc tgttgtcaaa
    37741 caagactgcg atacagcttc gattacttca gcattttttc tcaatgccgc ctcaccgaaa
    37801 taaccatcta caaataattg ttgaataaat gttctcggat aatacgtccc ttcatcaact
    37861 tctactaaat gaggatctaa ttgttctttg ataagcgttt ctaaagtcat catcatctct
    37921 ccttattcac caaatcttct aagtataaac tgctcaatat aactaatgaa tcgatcaata
    37981 ataaaaccaa ataatccgat aaagaatatt gctgctaaaa catcttctaa gttcaacata
    38041 tttcgtgcat caacgattaa aaaacctaat cccgattgtg caccaatcat ttcaccagaa
    38101 actaaaaata tccaacttgt tcctaccgcc atatgtatcc cagccatgat ttgtttaaat
    38161 gccccgggaa ataatatatt gcgatacaat gaccacccag ttaaatttaa atttgccgct
    38221 atttttaaat attgaggttc aatgtctcta acgcctttaa tagtattgaa cacaattggg
    38281 aaaaaagcag cgataaaaat aatcgcaatc gctggcaaac taccaatacc aaaccataga
    38341 acaacaaatg gtgcccatgc tatcggagat atcggtctaa tcaattgaaa tagcggttcg
    38401 atagcgttgt atagccaacg attccttcca agcaagaagc ccaatggaat agcaaccaac
    38461 aatgcgacaa caaagcccgc tacaaatctc cataaactaa ttgctaaatg ttggaaaatt
    38521 tctccagtaa caatgaaagt ccatatactt tttcctacaa gagcaggacc aggtaacaat
    38581 acaggttggt aatgcccaat aataatgacc atttcccaaa tgcctaagaa aataataaat
    38641 gtgataatag gtaatataaa tttgttattt gtgggacgtg tcatgaacgc gatgcctcct
    38701 tatacaatga cggttcaaca aagtcatcat atgcaggtgg attaaacaaa tgatgttgtt
    38761 ttaccaatgt cgtaatttct tgatagccgg atggcttaat tgttaaatca ccataggatg
    38821 tccatgccgc tgactgtgtt aaaacgtcac gactttgttt aaaatgatgc gtcataatgt
    38881 ctacactttg cttgcgatca ttcattttaa agccagactt tttataatct tgtacaaatg
    38941 cttgcgctac atccttgtgt tgattaagca attcccctct cagtactagc acacagcaat
    39001 acgcatcagg tataacgtca tcaccatgtt tcaaagtctt acctttgcct aacttttcac
    39061 ccagtgcacc gaatggttcg gctacagaat accctgtaat tctgtgttca ctcaatgcgg
    39121 ctggcatttc tgctggcgac atttcatgat agctaaaatg ccccggttta atctttaatt
    39181 gtttacgtaa ttcctcaagt aaaagataat gtgttgaata acgatgtggt ataccaaaat
    39241 ggtaatcatc gccattatta ttaaattcat ttaagtgcat acctttttgt cccataatga
    39301 cattgccttc atgatggccc aatgccacag cctttatatt tgagcccttc tgttttgatt
    39361 tcatcgcgag ctctattaaa gttgatgcac catcaatacg accactgttt aatgcgtcca
    39421 ttaaatctgg ccaattattg aatttaacta attctagttt atatttcgga tgattgtatt
    39481 gtgataataa ttttttagtc atcatcaaat tagctgaatg tgtaatcggc aaatatccaa
    39541 ttttaatcac ttgctgattt tgggtatttt tagacggttc tttagacgtc ctttgccaat
    39601 cacatcctgt aattataaag attccaatga tgacgattat gcttaacctt ttcatcgtca
    39661 ctcactcctt ataaataata ttcaggttca acttgatgat gattcaatgc aaatgtttcc
    39721 ataatttcat tacgaatctt aagtaggtgg ctatcattac gactgcgtgg atgtgatgct
    39781 gtaatttcat attgagaaat aatattgcac ccttcaccta acagaacaat gcggtcggaa
    39841 agataaatag cttcatcaat gtcatgcgtc actaaaataa tagttgattg cgttttatgt
    39901 tttagttgca ctagttgatc ctgaagttta taacgtgtaa atgcatctaa tgcacctaat
    39961 ggctcatcca tcaatataac gttaggctta tgcacatgcg ctcgacatag tgccacacgt
    40021 tgtttcatac ccccggacag ttgctcggga aaatgctttc ccctgccttc taaatcaact
    40081 aatttaagct gtgcgttaat ctcttcatca ctaattttct gttgaaatcc aatcctaatg
    40141 ttgtcattaa tcgttttcca tggcagcaaa ttatgatgtt gaaatagcat taaacaatct
    40201 ggagacggct gttgtttaat ttcgttatca ataatgacac gcccagatga tggatgaata
    40261 aatccaccga taatattgag taaagtagac tttccgcaac cactttttcc tatgaaagtg
    40321 actatttctc ctttgctaat gtccaaatta aagttatgaa ttactttatg tgatccaaag
    40381 tgatgttgta attgttgtat tttaatcatg acaagccccc ttttaatccc tactatttta
    40441 ataggaatta agtgtatatc taaagggcta tgccaaacac agcccttata tcgtgaattg
    40501 ttatatctat agatgattat ggtttggtat gagtgctctt ataacataag cgttattcaa
    40561 ttaacgacgt tatattaatt tcagctctac taaatttcaa taaaatgatt gttttagaca
    40621 atacttgcat ttagttcatt agtttcttca tatcaactac ttacatttat agcattgagc
    40681 tattctaatt aactgcaatc gaaatccacg tcaaacctat atacatatat ctcatcaact
    40741 tgttgtctta ttaaagtttt tcgatgccat caatcggtgt atcaactgct tttcgcgcgc
    40801 gacgtaccgc ttcttcatca ggtgcgttgt ataaacaaat acatttagac atatcttcac
    40861 atacatatgt gcgtttaaat tcaacttctg gcacttcttc ataatgaaca gaatttttct
    40921 ttttacgtgc taaatattga tccatcgtaa tgccttccgg aatgttccaa gttacaaggt
    40981 aatcaacatg tgcatcacca tttttaactt catctaaatc tttaccaact aagcgtactt
    41041 ctttgactaa ttgaatacta atatctgctt ctgttaaaaa ttgtttagct tctgcttcgt
    41101 catttgcttc cacaataaaa taaccatgag ttaaattttc agtagcttgt acttcaatta
    41161 atgttggaat tgtcttcgta gatagtgatg ctgcctttgc ttctagttct tctttcgttg
    41221 atgcaaaatc aagattgtta gcttctaata aaaataatgt cattaataag acctcctata
    41281 acaatggttg aaaaaatatt actataattc ttatcggatt acaaggatat tgagcgattt
    41341 ttttaaatat ttagataaaa ttctgtaaaa tatattttca aagagtataa aaatcatgtt
    41401 agggtataag taaataaaca tatatatagg gggctatcat tatggataaa gaatctgtcg
    41461 tagcaagttt agctagaaac aaaaagattg ctgtagaaac aatggccggt caaagataca
    41521 tcatagaacg cattttacat acaaatgatg aaaaacatat tcatattttg aaacctaaag
    41581 atgtcgtgtt agatgtagat agcatcaaag aaattgacga aaatcattta aatgatgcaa
    41641 cataagtata aaacgaaacg tgcgcctatg acatagatta atgttttagg cgctacaatg
    41701 ttttatttat gttaatttgt cgaagtatgg ctcgtctaat ggattcaatt gcaagttttc
    41761 aatttgcaac gttgaatgtg caatgccata tttttctttc aacaatgatg atacttgatc
    41821 aatcgcttga taatcatcac cctcatattt tttatctaac acaacatggg cacttaatga
    41881 ataatgctct gttgtaatac tccacaaatg aaattcatgt acatctaata tgccatctat
    41941 gtttttaata tctgccataa tttgatcagt atccaaatgt tgaggcacac tttccattaa
    42001 aattaaccac gcattacgcg taattttata accaccacgt aaaatgatga gtgaaattac
    42061 aatactaatg attgggtcga tgatgcgcca tcctgtaaag taaatcaata caactgcaac
    42121 gatgacacca atagagttca ataagtctcc catgaaatgc cataatgcac tttgaatatt
    42181 gatattgtct tcttgtttta aagaccttac aaggataaca gtcaaaataa tattgacgag
    42241 taaaccaata ctagcaatca taaacataat gccactttca attggttgcg gataaataat
    42301 acgtacaata gcttcatata aaatccagat tgaaattaca attaatgcta aaccatttaa
    42361 aaatgcagct aatatctcaa atcttaaata tccaaatgtg tatcgtgcag tcggcttttt
    42421 acttgcaaaa taaatggcca acatagataa accaagtgct aatacatcac taagcatatg
    42481 aaatgaatct gacagtaatg ccaatgaatt agatactaaa cctccgacaa attcaatcac
    42541 tgtaaataac aatgtgatga taagtgatgc ccacagcgtc tttttagaac tttgttgaat
    42601 tttttgatga ttgacatgat gaaaatattt gtatctgttt atcgtattcg cctttttccc
    42661 attcatacgt ttgccactcc taaataaatg cgtcgtattt aatattgtcg cttttaaaaa
    42721 taattcttta tagcttctca ggttaatatt aatgcttccg taattaacca tgatttatac
    42781 atcatcgtca tttacatgac aactcattaa tctttaccct ttatagcttt atgtaatatc
    42841 accttggaaa acttttacag cacactcgcg ctttgcttta ttcctatcat atagaaaatt
    42901 ttaataatgt acgagattat tgtgttagtt acataaaaat gacaaaaaag tatctcaaat
    42961 ttattgttta gtttcattaa gttcaaaaaa tcgcaataac tttaatgatt gttccgcata
    43021 ttaaaacaaa cgatgacttt gcatattgaa cttcatattt ctaaatcacc ataattaata
    43081 aacaactctg tccttataat agccgcatcg caaattagca taattaaaaa cagcagtaag
    43141 atgatttgca attaaaaacc atcctactgc tattatttta gtcattatat tctaatatca
    43201 tgatacgtgt tcctatatat tttgttttgc ttaaaatcta aacaacgccg ttaaatgtta
    43261 agacttaccg ctagactgac ctacaaacac gcttcagaaa cgatatttat tctagtacaa
    43321 accttttaaa gcattgcttg tatcaatata aatattttta acttgttgat agttacttaa
    43381 cgcaccttta taagtttctc gaccgatacc tgatttttta taaccaccaa atggtgcgcc
    43441 ttctggtact tggttgtaag tgttaatcca aatacgtcct gtacgtacag ctttagcaat
    43501 atttaatgca cgtgtgatat tttgagaaaa tacaccgcct gctaaaccat actcagaatc
    43561 attagctata tcaattgctt cttgatcgtc cttcacttta atcactgtta acactggtcc
    43621 aaatatttct tcttgtgcta atttgtgatg attgtctggc acagcaatta atgtcggctc
    43681 aaagaagaac cctttatcta atccattttc agttaagcga tgaccacctg ctaaaatttg
    43741 tgcatctgat tcttttgctg catcaatata tgattgaatt ttatctaatt gatccttacc
    43801 agtttgacta cccatttgtg tagcttcatc ttgtggatct ccaactttaa tatttgaaaa
    43861 tacctcttgt aaacgtggca ccaattgatc ataaattttt tcatgaacta ataatcgaga
    43921 acctgcacta catacttcac cttggttgaa taaaatacct aactgaatac cttcaactgc
    43981 aaggtctaaa ttagcatcat ctaatatgat attggcgctt ttaccaccaa gctctaatgt
    44041 agcgggtact agatgttttg ctgcagcttc ggcaacttga taacctacat cagttgagcc
    44101 cgtaaatgat aatttatcta caccatcatg attgaaaatt gcattacctg attctgaacc
    44161 tttacccgtt agtatattga caacaccttt aggtaatacc tcttggaaaa ttttagcaac
    44221 ttccaataaa cttaatggtg ttgaagacga aggttgaatc acaattgtat tacctgcagc
    44281 aatggctggc gcaatcttcc atgcagctaa tagcattggg aagttccaag caacaacagc
    44341 acctacgacg ccaatcggct catgtcttac gatactcatt gtgtctttat cgatatcatt
    44401 tactgtacct tcttctgttt caataacact tgcgaaataa tggaaatgtc ttgcagcaaa
    44461 tggaatatca attgctgttg tctcacgaat cggtttacca ttatttaatg tttcaatcat
    44521 tgcaatttta tctttttgtg ccattaattt atcaccaata tcacgcaaca tttgtgcacg
    44581 ttctgattta gaagttaatg accatgattc aaatgcctct tgcgccactt tgactgcatg
    44641 atcgacatct ttatcttttg ctcttgtaat atgtgatagt gtttctccag ttgctggatt
    44701 agtcacttcg attgtttcgt cactgctacc tttaacaaat tccccattga taaataaacc
    44761 ataattctct gcaatataat ctcgaacgtt tactgccata ttatctctcc tttgtttatt
    44821 ggctataata taaatctctt agcatcatat ttaataatgc ttttaaacga tatgattcac
    44881 attatatagt aagcgtttac attttataaa ataatcagct cacagaagtc tcattttaaa
    44941 acttttgcgt cgtttttgca cataaaaata atcttatttt cgaaaacata ttgcactttt
    45001 caaaaataaa acacataata taactataat acttgagaac aattctcatt aaatagtttg
    45061 gttgttatga aagtcatgct taaaatgcgt taataggaca aggataattc aacaacgttt
    45121 cattttactg agtcattgca cttatcatac acattatatt tagcatgagt tatattacta
    45181 aaacaatatt acatgcgcat tagtactaga atggagtttt ttcaatgcga cttattttaa
    45241 tcgttattgg cttaatattt acagcgcttg gtattgcagg tgccgtatta cctttactgc
    45301 caacgacccc ttttttactc gtagcagttt tttgttttgc tcgaagttca gatcgctttt
    45361 acaattggct tattaatcaa aaaatttata aagaatatgt agaaaacttt tatttacatc
    45421 gaggctacac gctacaacag aaaattaaaa ttttaattag cttatacatt gtaataggtt
    45481 tttcaattta tatggtggat gttcttgcag tccgtgtagg attaatcata atggttatca
    45541 tacaaaccgc tgtactcttt acatttgtaa aaacattacc caaatcaaat cataaaatag
    45601 aggagtgatt gcccatgttt atggcagaaa atagattaca attacaaaaa ggcagtgcgg
    45661 aagaaacgat tgaacgtttt tacaatagac aaggcattga aactattgaa ggcttccaac
    45721 aaatgtttgt cactaaaaca ttaaataccg aggatacaga cgaagttaaa atcttaacta
    45781 tttgggaatc tgaagacagc tttaataatt ggttgaattc cgatgtattt aaagaagctc
    45841 ataaaaatgt acgtttaaaa agtgatgacg atggacagca aagtccaata ttatcaaata
    45901 aagtattcaa atatgatatt ggctaccact atcaaaaata aatatattaa aaacccctag
    45961 tcaccgctgg ttgtctaaac caacttcatg attaggggtt ttttattatt tgtgacgtaa
    46021 aggtacgaat tcatccggct tgtctgtgcg caatccaaaa taatattcta ttgcttcaca
    46081 aatacgtcgt gatgcttgtc catcaccata tggattattc gcttgactca tacgttgata
    46141 tgcttcatca tcctcaatca attgtttcac atttcgaaca atattgtcat aatctgtgcc
    46201 aattactctc gatgttcccg cctcaacgcc ttcgggacgc tctgtatgat tccttaatac
    46261 caacacaggt tttccaaatg taggagcctc ctcttgaata ccaccagaat ctgtcagcac
    46321 gaggtacgat tgatttgtaa aattatggaa ctcaatcgca tctaatggct caattaattc
    46381 aatccgattt ctcccagata aatatttttc ggcaatcgct cttacctttg gattacgatg
    46441 cattggataa atgaagacaa catctttata ttcatctgcc aaatctctta ctgctttaaa
    46501 aatctgatgc atcggttccc caatattttc acgacgatgc gctgttagta aaataacttt
    46561 cttgccttta tgtttattaa taatcgttga aacaaaatca ttttgaactg ttgttgacaa
    46621 tgcgtcaata actgtatttc cagtaataaa gatacgctct ttgtctttgt tttcaaaaag
    46681 taaattttta gctgcaatta ctgttggcgc aaaattcaat tcagcaatat tacttaccat
    46741 gactcgattt aactcttcag gaaatggtga gtatttctga tgtgttcgaa gtccagcttc
    46801 tacatgtccg accggaattt gatgataaaa tgctgccaaa cttcctacaa aagtcgttgt
    46861 agtatcacca tgtactaaaa tcatatccgg ctgttcctca ttaatgatgc tatcaagttt
    46921 agcaagcgca ttagccgtaa ggcccgctaa cgtttgttga tcttgcataa tatttaaatc
    46981 atgatcagct tgaatatcaa atatacttaa cacactatct aacatatctc tatgttgtgc
    47041 tgtaatcaca atgttcgctt caaagttccc attatgatca atttctttta ctaatggtgc
    47101 catttttatt gcttcgggtc tcgtaccgaa aataaccata atttttttca taacattatc
    47161 ctctctgaag ttcaaacaca tgacagctta tctttcctta atcatgtatg ccctagtttg
    47221 acacatttta tttgtcgata aaattaaata tattgccata attataatac gatacatctt
    47281 caaatgatga tttcacaaca ttttttgtat caaaaatcac tttatgcttc attttatcaa
    47341 aatgactgtc cgataaattt ttaaattctg agtggtcact taaaatcaat actagcgatg
    47401 cgtctttgac agcatgtgac atatcatgtt ccacaaaatc taattcaaca tgtggatcat
    47461 aagcacatac ttctatgtct ggttcttgat ttaatagctc ataaatatca aatgctggcg
    47521 attctcttat atcatcaaca tcacctttat aagttaaacc aaatactgtg actttattcc
    47581 cgctcaatgc tttgatgatt tgcttcgttg tatcaacaac ataggccggc attgaattat
    47641 taatttcacg tccagtttga attaactttg cattttcagg gtctttagca ataataaagt
    47701 acggatcaac agctaaacaa tgaccgccta cacctggacc aggttgatgg atattaacac
    47761 gcggatgttt gtttgccatt tcaatcacat ctaatacatt aatatttaag ttgttgcaaa
    47821 tttttgttaa ttcattagct aaagcaatgt tcacgtctct atatgtgttt tccattagct
    47881 tactcatttc agcagtacgt gcatctgttt caatcatttc tccctgaaca aatgtgcgat
    47941 agacacgttt acccgcttca atacaagctt tagtcacacc gccaatgatg cgattgttat
    48001 gaactaattc ttctaaaatt tttcctggca gtacacgttc tggacaatgc actaaataaa
    48061 tatcttcacc tattgtaaac cctaaatttt caatgactgg ttttacaaaa tcatccatcg
    48121 ttttaggcgc aattgtcgac tctacaataa tagtattttc tttttctaaa aatggtaaaa
    48181 tactatctaa tgcacgcata actagcgaaa tgtcacatga ccggtactga tcatcattat
    48241 tcggcgtcgg aacggcaatg ataaaaacat cagatgcttc tggcgttgta gataccttca
    48301 attttcccga tgacagtacc tcttcataaa tctcttgtaa tccaggttct tcaatactaa
    48361 tttgaccact ttgtaactta tcaatcgttt gctgattaat atcaacacca agcacatcga
    48421 cgccatgctt tgcaaacata attgatgttg gtaaaccaat ataacctaag ccaactactg
    48481 ttaacttcat actataacct ccaaataaaa cgatttgtat gttgatttta tttaatagat
    48541 gacttatgcc ttatctttgg ttgtcgtttc atccatgatg tccgcaataa caagtgacat
    48601 ttttccagga ataacttcaa gtgcattatt attttcatat aacgtattgc tgtatattaa
    48661 attgccgaat aattttctaa agaccgacaa cttattaaaa tacttattta gcattgaagg
    48721 catgttgatc aataccgttt tacgatgtga ttggcgacgt atttcataca ttactgacga
    48781 tgtatcaaag taaaaactat cttgaggatg atacacgcct gtcacttcta atgatattaa
    48841 ttgatcaata aatgctgtca gatgtttaat atataatgca ctgcgctgat tgttaatatt
    48901 gggaatgatt ggcagtcgct ttgacaattg cattaaccgt tggaaatttc ctgggcaatg
    48961 tgcaccataa atcattggtg gtctcacaat tgctacttta aacgaatcac taatcaactc
    49021 ttgtaatgct tgttcagcga actttttgga aataccatag ttggtcgtag gattcattgg
    49081 tgtttgtgta tcaatttgat ctgatttacc aacatgacct tcttttccat aaactgccat
    49141 agtactcata aaaataaatt gtttaacgtc ttcagcttta gccttttgtg ccaattgttt
    49201 cgtcagcaac atattcactt gcatataatc agatagcctt gcttgaggtg aattgttgtg
    49261 aaccaaagct gctgtatgaa ttaaaacatc ataatctttg aacgaggtcg acttccataa
    49321 ttgattccta acattaattt gatctacttg atgtccttgt tcaataagct tatcttttaa
    49381 agcattaccg atatatccat gtacgcctgt aattaaaata ttttttctca ttagtgatgc
    49441 acaccttctg aagtaacgat atttttaatt gttttatata tgatatacat atcaagcatc
    49501 atagattgat gtgttaagta ataatgatca tacgctactt tttgatcatc agtgatatca
    49561 tctctcccca tcacttgagc tagtcctgtc acacctggtc taatcgtatg cacgttcgct
    49621 tttgtacgtt tttcgattaa ttcgtattga ttataaagcg ctggtctagg acctacaatt
    49681 gacatttctc cttttaaaac attcaataat tgtggcaatt catcaataga ggtcttacga
    49741 atgaccttcc ctgtctttgt tatatacgat gttgaatcca ttaaatcagt tgcaacatta
    49801 ggtgtgtcta ttttcattga tctaaactta taaatattaa acaattcatt attaatcgtc
    49861 ggtctttttt gtttgaaaat ggctggtcca ggtgattcca ttttaattag taatgctgta
    49921 attaacagaa tcggacttaa aactactaaa ccatatattg aactcactac atcgaataat
    49981 cgcttcataa ttatctcctc acttcaacag attgtaatac ttcatagtat taatttctct
    50041 cgtatattta tctttaaaca gttgatgtgc gcgctcaccc atctcgtgca attccttact
    50101 cgagtgagtg ttgatgaagt tataaatggc atgtgcatcg ccattatcaa tttggatacc
    50161 cgcatcatat tgttcaacat gttgaacgat atcagattgc ttatccatga tgagtaccaa
    50221 cgctttctta gctgcaagat agccataatt cttgctcggc acgcctaaac cgacgccttc
    50281 tttaatcagc gatgcaatac atacatccgc aatttttaat acgtcagcat agtctgtacc
    50341 tgttaaaaac tcaaacattt taacattttc aatacgatgg tcttctattg ccgttttgac
    50401 atctgcaaat ttcttaccat gaccacaaag tattgttaac gtttgaggct gatccttatt
    50461 taattttaaa aatgagataa gtgtctccat atcctgtaac tgccccatat taccgctata
    50521 caataaaatt ttgtcgtatt gctcacggta agctttaaat gtgtcattat agatacgatt
    50581 gtcttgtaat tgacgcatgt cataccagtt aggaatcaca tggatattgt cagcattttt
    50641 agaaatttga tgatttagta agtagttttt catttccgta ccaaggacaa tgacattttc
    50701 agcattcttg tagacatgtc tattaatgta acgcatcagc ttatcaatca tgctacctgg
    50761 acgagttgca cctgtcttaa tcgcattatc aggtgctata tcatacacca caaaagaata
    50821 ttttttctta agcagtctgt gtaaaacgtc tggtattaat ggcaagattg gtggattaga
    50881 gtaaacaaga atctgatcat atttcaacat tttaggtata ttaatcacga attttgaaaa
    50941 taaactaaag aaattgatga tccttccaac cttacttttg ttattaaacc tcgaatactt
    51001 gagacgtcga atgcgaatac cacgatgcat ctcggtttta gaaacctgtt tatgattact
    51061 atattcatat ggccatccac acatgacatc gacattaatg tgattcgcaa ttaaatcttc
    51121 cgccaattga gttggtaacg tcgcagaaga tacatattcc ggataaaaat actgacataa
    51181 aatcaaaatc tttttttcac tcatctaatc gacgtccttt ttattaatga atttagacta
    51241 agaatttttg aactgtttat ttttagcaaa ataatagagt gctatgaaaa atatcatgta
    51301 cgatacgatt aaaacaattg tattaataga tacaaagttt aaaaaagtta aattaagcat
    51361 ttttgatata acaaatgttg cagtagtacc tattaaaaat aacgcttgta gtattaattc
    51421 taactgttgt ttttgtaata caatcacaga aagtgatacc gttgatacaa tcagccttat
    51481 aacaaataat gggataacaa tttttataac tgtaattgcg tcaacccagc tgtgtcctaa
    51541 taatatattg actaattctt cactgaacaa ataaagtggc acataaagaa ttatagaaac
    51601 tataaccagt atggaactaa atttaagtaa taaatttcgg aagttaccat gttcaatata
    51661 atagtcattg gcttgttgca taaatatttt tgataaccct aatgaaataa ttgtcactgg
    51721 tatgcctagt actcttatgg ccataccata aatacccact tctgtattgg tatacaaaat
    51781 acctataaag aacacaacaa ccgagaaaga caaactattt aataatattg atggtgttga
    51841 ataaactaac tgatttttat tttctaaaaa taatgcttta gtttcttctt tatctctcac
    51901 aatattaaag tgctttttaa acgttttata tcctagtgta atgcctgcga tataggataa
    51961 tgaaaaacca ataattaatc ctaaacttcc taatgctaaa agtccgaaaa taatttgaag
    52021 taaagccatt gatccagctc taaacacatt aatcaaactt aacactttat actttctttc
    52081 cttatttaga taatttgtaa aaa
//
LOCUS       DAHHYJ010000003        47378 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000003 DAHHYJ010000000
VERSION     DAHHYJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 47378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.003"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(1..186)
                     /locus_tag="NBK22_000101"
                     /pseudo
     CDS             complement(1..186)
                     /locus_tag="NBK22_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            312..662
                     /gene="sarV"
                     /locus_tag="NBK22_000102"
     CDS             312..662
                     /gene="sarV"
                     /locus_tag="NBK22_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCD2413325.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(764..1975)
                     /locus_tag="NBK22_000103"
     CDS             complement(764..1975)
                     /locus_tag="NBK22_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2413326.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETDVINKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(1968..2408)
                     /locus_tag="NBK22_000104"
     CDS             complement(1968..2408)
                     /locus_tag="NBK22_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCD2413327.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHKILLKEAHND"
     gene            2570..3334
                     /locus_tag="NBK22_000105"
     CDS             2570..3334
                     /locus_tag="NBK22_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2413328.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRAKK
                     LQKYFQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFIRGAIYRFEPLV"
     gene            complement(3326..3577)
                     /locus_tag="NBK22_000106"
     CDS             complement(3326..3577)
                     /locus_tag="NBK22_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413329.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLVIIHMGILAHSTYVYLY"
     gene            3966..5231
                     /gene="femX"
                     /locus_tag="NBK22_000107"
                     /gene_synonym="fmhB"
     CDS             3966..5231
                     /gene="femX"
                     /locus_tag="NBK22_000107"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCD2413330.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            5348..8515
                     /locus_tag="NBK22_000108"
     CDS             5348..8515
                     /locus_tag="NBK22_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCD2413331.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASASNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVVTIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDSDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGNIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(8699..9016)
                     /gene="mspA"
                     /locus_tag="NBK22_000109"
     CDS             complement(8699..9016)
                     /gene="mspA"
                     /locus_tag="NBK22_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCD2413332.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(9261..9425)
                     /locus_tag="NBK22_000110"
     CDS             complement(9261..9425)
                     /locus_tag="NBK22_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413333.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLNLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            9558..10466
                     /locus_tag="NBK22_000111"
     CDS             9558..10466
                     /locus_tag="NBK22_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCD2413334.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVAGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     PFIGMTTNLTIIISIIILYVYQAVFV"
     gene            10872..11003
                     /locus_tag="NBK22_000112"
     CDS             10872..11003
                     /locus_tag="NBK22_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413335.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(11120..11983)
                     /locus_tag="NBK22_000113"
     CDS             complement(11120..11983)
                     /locus_tag="NBK22_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCD2413336.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(12164..13051)
                     /locus_tag="NBK22_000114"
     CDS             complement(12164..13051)
                     /locus_tag="NBK22_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2413337.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IKSPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFETLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            13116..13211
                     /locus_tag="NBK22_000115"
     CDS             13116..13211
                     /locus_tag="NBK22_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413338.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            13237..15372
                     /locus_tag="NBK22_000116"
     CDS             13237..15372
                     /locus_tag="NBK22_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD2413339.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWTSSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYYTLSL
                     TVKGFDFQLESNQRYTNKETLEQIVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            15485..16819
                     /locus_tag="NBK22_000117"
     CDS             15485..16819
                     /locus_tag="NBK22_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCD2413340.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(17010..17399)
                     /locus_tag="NBK22_000118"
     CDS             complement(17010..17399)
                     /locus_tag="NBK22_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413341.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLIFN
                     ALLYLILTIILLVNKQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            17756..18064
                     /gene="rpsJ"
                     /locus_tag="NBK22_000119"
     CDS             17756..18064
                     /gene="rpsJ"
                     /locus_tag="NBK22_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCD2413342.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            18092..18754
                     /gene="rplC"
                     /locus_tag="NBK22_000120"
     CDS             18092..18754
                     /gene="rplC"
                     /locus_tag="NBK22_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCD2413343.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            18781..19404
                     /gene="rplD"
                     /locus_tag="NBK22_000121"
     CDS             18781..19404
                     /gene="rplD"
                     /locus_tag="NBK22_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCD2413344.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            19404..19679
                     /gene="rplW"
                     /locus_tag="NBK22_000122"
     CDS             19404..19679
                     /gene="rplW"
                     /locus_tag="NBK22_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCD2413345.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            19712..20545
                     /gene="rplB"
                     /locus_tag="NBK22_000123"
     CDS             19712..20545
                     /gene="rplB"
                     /locus_tag="NBK22_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCD2413346.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            20612..20890
                     /gene="rpsS"
                     /locus_tag="NBK22_000124"
     CDS             20612..20890
                     /gene="rpsS"
                     /locus_tag="NBK22_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCD2413347.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            20919..21272
                     /gene="rplV"
                     /locus_tag="NBK22_000125"
     CDS             20919..21272
                     /gene="rplV"
                     /locus_tag="NBK22_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCD2413348.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNAAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            21296..21949
                     /gene="rpsC"
                     /locus_tag="NBK22_000126"
     CDS             21296..21949
                     /gene="rpsC"
                     /locus_tag="NBK22_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCD2413349.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            21952..22386
                     /gene="rplP"
                     /locus_tag="NBK22_000127"
     CDS             21952..22386
                     /gene="rplP"
                     /locus_tag="NBK22_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCD2413350.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            22376..22585
                     /gene="rpmC"
                     /locus_tag="NBK22_000128"
     CDS             22376..22585
                     /gene="rpmC"
                     /locus_tag="NBK22_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCD2413351.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            22609..22872
                     /gene="rpsQ"
                     /locus_tag="NBK22_000129"
     CDS             22609..22872
                     /gene="rpsQ"
                     /locus_tag="NBK22_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCD2413352.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            22904..23272
                     /gene="rplN"
                     /locus_tag="NBK22_000130"
     CDS             22904..23272
                     /gene="rplN"
                     /locus_tag="NBK22_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCD2413353.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            23308..23625
                     /gene="rplX"
                     /locus_tag="NBK22_000131"
     CDS             23308..23625
                     /gene="rplX"
                     /locus_tag="NBK22_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCD2413354.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            23652..24191
                     /gene="rplE"
                     /locus_tag="NBK22_000132"
     CDS             23652..24191
                     /gene="rplE"
                     /locus_tag="NBK22_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCD2413355.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            24214..24399
                     /locus_tag="NBK22_000133"
     CDS             24214..24399
                     /locus_tag="NBK22_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCD2413356.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            24431..24829
                     /gene="rpsH"
                     /locus_tag="NBK22_000134"
     CDS             24431..24829
                     /gene="rpsH"
                     /locus_tag="NBK22_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCD2413357.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            24854..25390
                     /gene="rplF"
                     /locus_tag="NBK22_000135"
     CDS             24854..25390
                     /gene="rplF"
                     /locus_tag="NBK22_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCD2413358.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            25421..25780
                     /gene="rplR"
                     /locus_tag="NBK22_000136"
     CDS             25421..25780
                     /gene="rplR"
                     /locus_tag="NBK22_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCD2413359.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            25801..26301
                     /gene="rpsE"
                     /locus_tag="NBK22_000137"
     CDS             25801..26301
                     /gene="rpsE"
                     /locus_tag="NBK22_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCD2413360.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            26318..26497
                     /gene="rpmD"
                     /locus_tag="NBK22_000138"
     CDS             26318..26497
                     /gene="rpmD"
                     /locus_tag="NBK22_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCD2413361.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            26514..26954
                     /gene="rplO"
                     /locus_tag="NBK22_000139"
     CDS             26514..26954
                     /gene="rplO"
                     /locus_tag="NBK22_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCD2413362.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            26954..28246
                     /gene="secY"
                     /locus_tag="NBK22_000140"
     CDS             26954..28246
                     /gene="secY"
                     /locus_tag="NBK22_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCD2413363.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            28263..28910
                     /locus_tag="NBK22_000141"
     CDS             28263..28910
                     /locus_tag="NBK22_000141"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCD2413364.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            29103..29321
                     /gene="infA"
                     /locus_tag="NBK22_000142"
     CDS             29103..29321
                     /gene="infA"
                     /locus_tag="NBK22_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCD2413365.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            29353..29466
                     /gene="rpmJ"
                     /locus_tag="NBK22_000143"
     CDS             29353..29466
                     /gene="rpmJ"
                     /locus_tag="NBK22_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCD2413366.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            29489..29854
                     /gene="rpsM"
                     /locus_tag="NBK22_000144"
     CDS             29489..29854
                     /gene="rpsM"
                     /locus_tag="NBK22_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCD2413367.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            29878..30267
                     /gene="rpsK"
                     /locus_tag="NBK22_000145"
     CDS             29878..30267
                     /gene="rpsK"
                     /locus_tag="NBK22_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCD2413368.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            30342..31286
                     /locus_tag="NBK22_000146"
     CDS             30342..31286
                     /locus_tag="NBK22_000146"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCD2413369.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            31303..31671
                     /gene="rplQ"
                     /locus_tag="NBK22_000147"
     CDS             31303..31671
                     /gene="rplQ"
                     /locus_tag="NBK22_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCD2413370.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            32209..33018
                     /locus_tag="NBK22_000148"
     CDS             32209..33018
                     /locus_tag="NBK22_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCD2413371.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVHKEGTAIEIFDHAEELTTIGLDLPFPIKINQMLGHQTSFLTYEGLVDQL"
     gene            33015..33875
                     /locus_tag="NBK22_000149"
     CDS             33015..33875
                     /locus_tag="NBK22_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCD2413372.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKEKLADWHIGLPEIVQLQYDFEQKYQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            33865..34671
                     /locus_tag="NBK22_000150"
     CDS             33865..34671
                     /locus_tag="NBK22_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD2413373.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFIMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVKRTSYRQLKWQLRDTLSLIMIIPIAIILFVLKYSGV"
     gene            34676..35479
                     /gene="truA"
                     /locus_tag="NBK22_000151"
     CDS             34676..35479
                     /gene="truA"
                     /locus_tag="NBK22_000151"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCD2413374.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKL
                     LESKNRKNVPFTATAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            35719..36156
                     /gene="rplM"
                     /locus_tag="NBK22_000152"
     CDS             35719..36156
                     /gene="rplM"
                     /locus_tag="NBK22_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCD2413375.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKVT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            36170..36568
                     /gene="rpsI"
                     /locus_tag="NBK22_000153"
     CDS             36170..36568
                     /gene="rpsI"
                     /locus_tag="NBK22_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCD2413376.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            36850..37032
                     /locus_tag="NBK22_000154"
     CDS             36850..37032
                     /locus_tag="NBK22_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413377.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSMYLMIIFL
                     ICLGTASIIYNYKTNR"
     gene            complement(37014..37178)
                     /locus_tag="NBK22_000155"
                     /pseudo
     CDS             complement(37014..37178)
                     /locus_tag="NBK22_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567545.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37476..39140
                     /gene="alsS"
                     /locus_tag="NBK22_000156"
     CDS             37476..39140
                     /gene="alsS"
                     /locus_tag="NBK22_000156"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCD2413378.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPVESKAISLCQKPSLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEE
                     TNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISVKVDEP
                     FINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            39177..39881
                     /gene="budA"
                     /locus_tag="NBK22_000157"
     CDS             39177..39881
                     /gene="budA"
                     /locus_tag="NBK22_000157"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD2413379.1"
                     /translation="MASVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIHDEDFTN
                     ANIDYKDIADEIREAE"
     gene            complement(40268..40693)
                     /locus_tag="NBK22_000158"
     CDS             complement(40268..40693)
                     /locus_tag="NBK22_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD2413380.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKELTMDGK
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKIKNELASQRGVTTEKINNAQIA
                     TYTLTLNDGNKKVVNLKKNDDVKNSIDSSTIKQIQIVVK"
     gene            complement(40882..43329)
                     /locus_tag="NBK22_000159"
     CDS             complement(40882..43329)
                     /locus_tag="NBK22_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HCD2413381.1"
                     /translation="MTYRMKKWQKLSTITLLMAGAITLNGGEFRSIDKNQIAVADTNV
                     QTPDYEKLRNTWLNVNYGYDQYDEKNDAMKKKFDATEKEAEKLLSSMKTESGRTYLWD
                     SAKDLDNKSADMTRTYRNIEKIAEAMKHKDTKLNTPDNKNKVKDALEWLHKNAYGKEP
                     VKKLEELKTNFSKSAPQKNTNLNWWDYEIGTPRALTNTLILLKEDFTDEEKKKYTAPI
                     KTFAPKSDEILSSVGKAEPAKGGNLVDISKVKLLESIIEEDATMMKESIEAFNKVFTY
                     VQSNATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETPFKDSNQND
                     TTLKSWIDEGFMPLIYKGEMMDLSRGRAISRENETSHSTSATVMKSLLRLSDAMDEST
                     KAKYKQIVKTSVKSDSSYKQNDYLSSYSDISKMKSLIEDSTISTNGLTQQLKIYNDMN
                     RVTYHNKDLDFAFGLSMTSKNVAHYESINGENLKGWHTGAGMSYLYNSDVKHYRDNFW
                     ATADMKRLAGTTTLDNEEPKENKNSDKTFVGGTKFDDQHASIGMDFENQDKTLTAKKS
                     YFILNDKIVFLGTGIKSTDSSKNPVTTIENRKSNGYTLFTDDKQTTASNINDQETNSV
                     FLESTDTKKNIGYHFLNESKITVKKESHTGKWSDINKSQKSDDKTDEYYEVTQKHSNT
                     DDKYAYVLYPGLSKDNFKSKASQVTIVKQDDDFHIVKDNESVWAGVNYSNSTQTFDIN
                     NTKVEVKAKGMFILKNKDDNTYECSFYNPESTNTASDIESKISMTGYSITNKNTSTSN
                     ESGVRFELQQTLNKDDN"
     gene            complement(43319..43492)
                     /locus_tag="NBK22_000160"
     CDS             complement(43319..43492)
                     /locus_tag="NBK22_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413382.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVDSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKRRHVNDI"
     gene            43737..44153
                     /locus_tag="NBK22_000161"
     CDS             43737..44153
                     /locus_tag="NBK22_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD2413383.1"
                     /translation="MKTIEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNYDDNVERLWERK"
     gene            44223..45071
                     /locus_tag="NBK22_000162"
     CDS             44223..45071
                     /locus_tag="NBK22_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD2413384.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
     gene            45292..45417
                     /locus_tag="NBK22_000163"
     CDS             45292..45417
                     /locus_tag="NBK22_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413385.1"
                     /translation="MTYKHFSIITLITGIIMHMTMYFVPFECRKMPLFMMIIFTI"
     gene            45372..45665
                     /locus_tag="NBK22_000164"
     CDS             45372..45665
                     /locus_tag="NBK22_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413386.1"
                     /translation="MSENAIIYDDYFYNLKAVKTHNIAKNVNKSLLNDKGVSIGKFTQ
                     KVKGKNPTWRDPKTKWTISKNKGQSHGGSYWKLINNKGKRIASLTKEGKILRE"
     gene            45679..46287
                     /locus_tag="NBK22_000165"
     CDS             45679..46287
                     /locus_tag="NBK22_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3885 domain-containing protein"
                     /protein_id="HCD2413387.1"
                     /translation="MYFDINNLDIKKLNFRKVKNAIHLSLSGDKYQFLEDGKTINNTY
                     FLAVYDNAINIFEDLFSPSDSINLVHIVYVYNEPYKKTNVIKKFTSLTIDELYYFKEY
                     KKEKDDNILCEEFSYKCSIKDIKYKKLLKAISNQDFKGLVPNINQNDNYSEIYIINNT
                     KNLIYHLYDDRGLWLAFNNNEDYARYSEKYNDLISEFDNEDI"
     gene            complement(46343..46544)
                     /locus_tag="NBK22_000166"
                     /pseudo
     CDS             complement(46343..46544)
                     /locus_tag="NBK22_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789088.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(46581..47321)
                     /locus_tag="NBK22_000167"
     CDS             complement(46581..47321)
                     /locus_tag="NBK22_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCD2413388.1"
                     /translation="MFILRNDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSNVIDRTLKHCDNCGGA
                     IRPDIVLYGEILDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLMTE"
BASE COUNT        16978 a         7241 c         8674 g        14485 t
ORIGIN      
        1 caacgaaata aattttgtga aaatgttatt tctgtcccac tcttaagaat tactacataa
       61 ataaatcttt aagggctctt taacattgtt gtcacactcc aagtcattga gttgtaatat
      121 atctttttta ggtataaatg ttgtcgaata aacaacaagt tgtccaaaag atataaatct
      181 aaacaagata tagccagcaa tttaatattt gtaatagata aaatgctaag tttgatatat
      241 aataaattta agtaattgta taataatatg aattacaaac atctaagaag aaacatagga
      301 ggcatcatat tatgagtaat aaagttcaac gttttataga agcagaaagg gagttaagtc
      361 agttaaagca ctggttaaaa acaacacata agatttcaat tgaagaattt gtagtccttt
      421 ttaaagtgta tgaagctgaa aagattagcg gtaaagaatt gagggataca ttacattttg
      481 aaatgctatg ggatacaagt aaaatcgatg tgattatccg taaaatctat aaaaaagagc
      541 ttatttctaa attgcgttct gaaacggatg aaagacaagt attctatttc tatagtactt
      601 ctcaaaagaa attgttagat aaaattacta aagaaataga agtgttaagc gttacaaact
      661 aaaaacttaa aaagcatgcc aatcagtaca tcatatttgc gtcttgggga cagacaaatg
      721 atgaatagag attggcatgc tttttatttt tgaatataaa tatttagttc atggcatttc
      781 tagttacatg acgtccatga attaagaagt aaacaagcat agtaatgatt gctaaagcgg
      841 ccataaagcc gaagatttca ctatatgaaa acatatgagt aaataaccca aggaatgatg
      901 gaccgaagcc gacacctgca tctagaccaa cgtaaaaagt agatgtcgcg ataccatatt
      961 taatcggagg tgagactttt atcgcaatag attgcattgc agatgataaa tttccatacc
     1021 ctaaacctag gcaagcacca gcaagtaata ttaaccagct ttgatagctt gaaattaagc
     1081 acacaaatga aaggaaaagc atgataaatg ctggatagac aataatattt tcatttttat
     1141 catccatcaa tctaccagca ataggtctag taattaacga tgctatagca tagcaaataa
     1201 agaaatagct tgctgcagtg actaggtgtc gctctaaagc aaatgcttgt aaataagtta
     1261 ggattgacgc ataggtaacg ccaattaaaa gcataattac agcaacagga atggcctctt
     1321 ttgcaataaa ttgatgaata ctaaatcttg gtttattaat gacatcagtt tcagttttat
     1381 tatttgttac ttcgaaatca acttttataa ataatgagat aatgagtccg agtatgccta
     1441 atatgacaca aataataaac agtaaatcaa ttgcgtattt tgtaataagt aacatgccta
     1501 gaaatgggcc aatcgctgta cctaatacta aacttaagga aaataaactg atgccttcac
     1561 tttttctatt aacaggggta acgtatgccg caatagtacc tgttgcagtt gtcacaactg
     1621 cagttgcgat accgtttatg agacgtacaa agattaaaaa agctaaagac ccatcaataa
     1681 aataaagtaa ttgcgtgata attaaagcaa ttaaaccaat aaataataat cgtttaggtc
     1741 cgatttgatt tacaaattta cctgtagcaa atcgaccaat taaagacccg acaataaata
     1801 gtcctacaac taatccggct aatgaatcgg aagcgtcgaa agcttgctta ctatagccag
     1861 caataactac aagtaacaaa tacatgcata ggtatacgat aaagttgaca aaaaagttga
     1921 ttgtaaaact tttggtgaaa ataggtgatt tatttaatga gccttgatta gtcattgtgt
     1981 gcctccttta ataatatttt atgaatttga ttcatcacat ttatggtttc ttgcatttga
     2041 tcttgcgaaa tgtttgtttt ggcaactact gcttgttgga gcttttgtac atcaagacaa
     2101 acaatctcat ataattcatg ccctttaggt gttaaagtta aaaacttttg tcgtttatcc
     2161 tctaaactgt tttcaactgt tataaggtca ttttctatta gagcttttaa agtttttctt
     2221 gcagtaggct tttcaattgc acgtctatgt gaaatttcaa taagagtagt gggctgatgt
     2281 ttagcgatat cgcgtaagat taaccattgg ccataatata tattgtgttt ttctaaaatc
     2341 ggctcggcta attttaaata ggggcgatat attgttataa aactattgaa aaattcttgt
     2401 gatagcatag tgacatctcc taagacaaaa tagttagctt agctaacctt tttacaacaa
     2461 tagtaattat aaaacgggag caattagaaa tcaatatata attattaaga gcaaaaatat
     2521 ttatactttg ttaaaataag cgtaattaca tgtaaatagg gggatactaa tgatattgaa
     2581 atttgatcac atcattcatt atatagatca gttagatcgg tttagttttc caggagatgt
     2641 tataaaatta cattcaggtg ggtatcatca taaatatgga acattcaata aattaggtta
     2701 tatcaatgaa aattatattg agctactaga tgtagaaaat aatgaaaagt tgaaaaagat
     2761 ggcaaaaacg atagaaggcg gagtcgcttt tgctactcaa attgttcaag aaaagtatga
     2821 gcaaggcttt aaaaatattt gtttgcgtac aaatgatata gaggcagtta aaaataaact
     2881 acaaagtgag caggttgaag tagtaggacc gattcaaatg gaaagagata cacataaaga
     2941 tggtaaggta aagtggcaat tgctttatat aatgaatcag gatgatgatg aaattaagcc
     3001 accatttttt attcaatggg aagaaagtga ttccatgcgt gctaaaaaat tgcaaaaata
     3061 ttttcaaaaa caattttcaa ttgaaactgt tattgtgaaa agtaaaaacc gatcacaaac
     3121 agtatcgaat tggttgaaat ggtttgatat ggacattgta gaagagaatg accattacac
     3181 agatttgatt ttaaaaaatg atgatattta ttttagaatt gaagatggta aagtttcaaa
     3241 atatcattcg gttatcataa aagacgcaca agcaacttca ccatattcaa tttttatcag
     3301 aggtgctatt tatcgctttg aaccattagt ataaatatac gtaagtgcta tgagcgagaa
     3361 tgcccatatg aataatgaca agcacaatgg aaagaatcgt taatatatta tttaatcgtg
     3421 atgacttaat taaaatgaaa aagattgata atataaatgt gaaaaagata agtataaccc
     3481 gtaaactaaa gtaattcacg gtgagaggtt gactcaatgt cataatgatt gcaacgatgt
     3541 tcataattat aaatagactt aaaataattg ttctcatatc aaacacctca ttgttagatt
     3601 attgacatta taacaggggt aattgtatat gaacattaat gtggttgctt gaggaaaaat
     3661 ttattcattg aagtcaagtt ggttcatttt agaaatgaat atcgtgttag atgatgaaag
     3721 tatattgaag tataggtaac tagttgaaaa gtattaattg tacgataaca ttaaatttaa
     3781 cacgaaacat agatataaaa tgattcacaa ttaaaatggg taaatttgaa cttgctaaac
     3841 tattaattgg agcatggaca tttcaaaaat aagagttcaa atcttacaca agctctgaat
     3901 cgacactata agatacaaac tgtataatta aaggtattgt taaatagaag gagatatcat
     3961 aaatcatgga aaagatgcat atcactaatc aggaacatga cgcatttgtt aaatcccacc
     4021 caaatggaga tttattacaa ttaacgaaat gggcagaaac aaagaaatta actggatggt
     4081 acgcgcgaag aatcgctgta ggtcgtgacg gtgaagttca gggtgttgcg cagttacttt
     4141 ttaaaaaagt acctaaatta ccttatacgc tatgttatat ttcgcgtggt tttgttgttg
     4201 attatagtaa taaagaagcg ttaaatgcat tgttagacag tgcaaaagaa attgctaaag
     4261 ctgagaaagc gtatgcaatt aaaatcgatc ctgatgttga agttgataaa ggtacagatg
     4321 ctttgcaaaa tttgaaagcg cttggtttta aacataaagg atttaaagaa ggtttatcaa
     4381 aagactacat ccaaccacgt atgactatga ttacaccaat tgataaaaat gatgatgagt
     4441 tattaaatag ttttgaacgc cgaaatcgtt caaaagtgcg cttggcttta aagcgaggta
     4501 cgacagtaga acgatctgat agagaaggtt taaaaacatt tgctgagtta atgaaaatca
     4561 ctggggaacg cgatggcttc ttaacgcgtg atattagtta ctttgaaaat atttatgatg
     4621 cgttgcatga agatggagat gctgaactat ttttagtaaa gttggatcca aaagaaaata
     4681 tagcgaaagt aaatcaagaa ttgaatgaac ttcatgccga aattgctaaa tggcagcaga
     4741 agatggaaac atctgaaaag caagctaaaa aagcgcaaaa tatgattaat gatgcgcaaa
     4801 ataaaattgc taaaaatgaa gatttaaaac gagacctaga agctttagaa aaggaacatc
     4861 ctgaaggtat ttatctttct ggtgcactat taatgtttgc tggctcaaaa tcatattact
     4921 tatatggtgc gtcttctaat gaatttagag attttttacc aaatcatcat atgcagtata
     4981 cgatgatgaa gtatgcacgt gaacatggtg caacaactta cgatttcggt ggtacagata
     5041 atgatccaga taaagactca gaacattatg gattatgggc atttaaaaaa gtgtggggaa
     5101 catacttaag tgaaaagatt ggtgaatttg attatgtatt gaatcagcca ttgtaccaat
     5161 taattgagca agttaaaccg cgtttaacaa aagctaaaat taaaatatct cgtaaattaa
     5221 aacgaaaata gattaacgac tgaaatctga acgctcataa gactgtcatt tgcgttcaga
     5281 tttttttaca caatatagaa tggttgagta aaatattttt gaatatagtg aaagaggggg
     5341 aagtactgtg ataaaaaagc tattacaatt ttctttgggg aataagtttg ctatcttttt
     5401 aatggttgtt ttagttgtct tgggcggtgt atacgcgagt gctaaattga aattagaatt
     5461 actaccaaat gtacaaaatc cagttatttc agttacaaca acaatgccgg gtgcaacgcc
     5521 acaaagtacc caagatgaaa taagtagtaa aattgacaat caagtaagat cgttggcata
     5581 tgtgaaaaat gttaaaacgc aatccataca aaatgcttca attgtaacag ttgaatatga
     5641 aaataataca gatatggata aagcagaaga acagcttaaa aaagaaatcg ataaaattaa
     5701 atttaaagat gaagttggtc aaccagaatt aagacgtaat tcgatggacg cttttccggt
     5761 tttagcatat tcattttcaa ataaagagaa tgacttgaaa aaagtaacga aagtactgaa
     5821 tgaacaatta ataccaaaat tacaaacggt agatggtgtg caaaatgcgc aattaaatgg
     5881 gcagacgaac cgtgaaataa cccttaaatt taagcaaaat gaacttgaaa aatatgggtt
     5941 gactgctgat gatgtagaaa actatctaaa aacggcaaca agaacaacac cacttggatt
     6001 gttccaattt ggtgataaag ataaatcaat tgttgttgat ggtcaatatc aatctgttga
     6061 tgcttttaaa aacataaata ttccattaac gttggcagga ggacaagggc aatctcaatc
     6121 ccaaagtgac aataaacaaa attcagccat gtcagacgtt aatcaggcat caccgcagca
     6181 aaattcaaaa gcgtcagcat caaataatat aagtggtatg ccgacagcga aactaggaga
     6241 tttagctgat attacagttg gtgatgtgcg tacttctatt tctaaaacga atggcaagga
     6301 tgcggttaat ctacaaataa ctaaagctca agatgccaat acagttcaag tagccaaaga
     6361 tgtacaacgt aaaatcgata cattcgttga tgaaaataaa gattttaatg tcacaaaaac
     6421 aatggatact gcaaagcctg ttgagaaatc actttatacg atggttgaaa aagcatcatt
     6481 aggtacaatc gtggcaatta tagttatttt gctgttttta agaaacattc gtacgacggc
     6541 aatttctatt atatccattc cgttatcact tcttatggcg cttattgctc tgaaattgag
     6601 tgatgtttca ttgaatatac taacgttagg tgcattaaca gtagcgattg gacgtgtgat
     6661 agacgattcg attgtagttg ttgaaaatat ttatcgacgc ttaacagatt cagaagaaca
     6721 actaaaaggt gaaaatttaa ttatcagtgc gacaactgaa gtatttaaac caataatgtc
     6781 atcgacacta gttactatta tcgtcttctt accacttgtg tttgtatcag gttcagtagg
     6841 cgaaatgttt agaccttttg cattggctat tgcatttagt ttattagcat cgttattagt
     6901 gtcaattaca ctcgttccag ctttggcagc aacactattt aaaaaaggcg ttaaacgtcg
     6961 taataaacaa catcaagaag gattaggtgt tgttagtaca acttataaaa aagtattgca
     7021 ttggtcacta aatcataagt ggattgtaat tatattaagt acattaattt tggttgtaac
     7081 tattgtattt ggagggccga gactaggcac tagctttatt tcagcaggtg acgataaatt
     7141 tttagctatt acttatacac cgaagcctgg tgaaacggag caagcagtgt tgaatcatgc
     7201 gaaagatgtt gaaaaatatt taaaacagaa aaagcatgta aaaacaattc aatactcagt
     7261 cggtggtagt agtccagtag atccaacggg tagtacaaat agtatggcaa tcatggttga
     7321 atatgatagt gacacgccta attttgatgt agaagcggat aaggttatta aacatgcaga
     7381 tggctttaaa catcctggag agtggaaaaa tcaagattta ggaacaggtg caggtaataa
     7441 atctgtagag gttactgtaa aaggtccatc aatggatgcc ataaaatcaa ctgtaaaaga
     7501 tattgaacag aaaatgaaac aggttaaagg actagccaat gtcaaatctg atttatcgca
     7561 aacatatgac cagtatgaaa ttaaagttga tcaaaataaa gcggcagaaa atggtatttc
     7621 tgcaagtcaa cttgcaatgc acttgaatga aaacttacca gaaaaaacag ttacgactgt
     7681 taaagaaaat ggtaaaactg ttgatgttaa agtcaaacaa aataagcaaa cagactggtc
     7741 agaagataag ttgaataata taactttgaa aaagccgact ggtggtacga ttaaattggg
     7801 aaatatcgct acgttagtta aaacaacgac accaagtaaa ttgacgcaag aacaaggaga
     7861 ttatgcaacg acggtatctg ctaaagtaac aaataaagat gtgggtggca caacacgaca
     7921 agtgatgtct aaaataaata atttggacaa accgaataat gtaaaggtta atatcggtgg
     7981 tgcatcagat gatattaaca atgcaatgac acaattagcc tttgcaatgt tagctgcgat
     8041 cattatcgta tatttaatcc tagttattac atttaaaggt ggcttagcac catttacaat
     8101 tctattctct ttaccattta cagttatcgg tgtaattatt gcactattaa tcacaggaga
     8161 aacaatatca gtaccaagtt taattggtat gctaatgtta attggaatcg tagtaacaaa
     8221 tgccattgtg ttaatagacc gtgtcattaa taatgagcag cagggcatgg agatgaaaga
     8281 agcattaatc gaagcaggcg gtactagaat tagaccgata ttaatgacgg cgattgcaac
     8341 aatcggtgca ttagttcctt tgttatttgg tcaagatagc tcgattctta tttcgaaagg
     8401 attagccgct acagttattg gtggtttaat ttcatcaact ttattaacac ttgtagttgt
     8461 accagtaata tatgaaattc tctttacttt gaaaaaacga ttcactaaac gatagataat
     8521 ataaaagcca taaaagcggt catgatattg actactttta tggcttttta aaatttgaaa
     8581 ttttagatct tatttcacgt gcgatttaat cgatgtgtta ataaaacaat gctggcattc
     8641 aacatgtacg ataataaaat aaaattgcaa ttatgttatt gcatgttttg tttattactt
     8701 agaataatac gatggttaag atgaaatata tgacaaagat aaataatgac attaaattca
     8761 agatgtttac accttttaga tcttttttaa gcattttaaa tcctgtcact tcgacattac
     8821 cgactaagag taataagacg ataaataccc accaattctc ttttggaaaa ctcatcgtcg
     8881 ttaatgctaa aacgaataat aacaacacac ccataataat tctcaaaatg cgagtaaata
     8941 caacttccat tttaaaaata ctaataaaac taacaattag ataaagtatt gctagtaaaa
     9001 tcagataaaa ttgcatatga taacttcctc tccgtttcaa agggttcatg tagtacagca
     9061 gcgtagaaaa cataaatagc agtgttggga tagatatgcg tgtatgttgt taatgaaaca
     9121 ctaaatcata acacgacaac atcttgtgat tctagatgta attgagcgaa aaataaagca
     9181 attcatcaat ataacgtatt cagagcaatg ctagtcaaat ttatgtaacg aaaagcattt
     9241 attgcttgaa tgatagatta ttaatcttgc atttgagcgc gaacgtttct aatttcattt
     9301 tcaatatctt taagttgacg aacaagtgcg gaagtatcta aattcaaaga ttcccgtttt
     9361 tctaagtcac cttgaagacg aacataatca tatttaagtt cggctagttg gttttctaaa
     9421 gacatactat aacactcctt tgaaatataa ttaagtgcat ttaatgtaga ttatagcatg
     9481 aaacagtaga cttctaaaat atctgtggag tatgttaaag ttataaatta tgaagattat
     9541 tgaaagaagg gataacagtg acagaacaat ttactatgat tattttatta attgcgcttg
     9601 gttacttttt gaagcgcatt aattttataa aagcaacaga tagtcaagtc attgctacat
     9661 tagttcttaa tgtcacactc ccatctttag ttattgtgaa tttaaacagt gcagaattaa
     9721 aattgtcatt ctccatttta ccaattttaa tgattatata tggtattgtg gcgaaaatga
     9781 ttgctgtctg gttttttaga aaatatgata atcacatgcg tggatcagta ggtatgatga
     9841 ctggtgctat gaatattgga ttgtttgcat atccactaat tgaagtgata tggcctaaaa
     9901 cgggacttat ttattttggt atggctgata taggtggtgc ctttgtgatg tttggaatta
     9961 cttactttgt aggtagctac tttagtgaag gtagtgatca attcgatttt aaatatttag
    10021 gtaagaaatt attacaatca gtgccactcg ttacatacat tgtaatgttt atattgaata
    10081 taagtcacat ccatttacca catgtggcaa tcgatttctt tagcatactt tcccatgcaa
    10141 atatgccgtt atcaatgatt ttgctaggtg tcatgttgaa ttttactctg gaaagaaagt
    10201 atatacctgc tactattaaa tatttatgtt tgcattatgg attagcatta gttgcgggat
    10261 tgttagtaca tgtgttgtta ccggtatcag atgatatgat taagacgaca ttgttggtca
    10321 cttggatgtt cccggttggt gtcgcaatta tttcttatgg tattcagttt aaatatcgaa
    10381 cactgccatt tataggtatg acgaccaatt tgacaatcat tattagtatc attattttat
    10441 acgtgtatca ggcagtgttt gtctagttta cacatttgga aataaaatcc attttaatag
    10501 tcgaagaaaa tgagagcccc ctttactaca acatgtaaat gcatattgtg aaaagggggc
    10561 ttttatttat gacatagttg ttcaacaggc tgggacataa atcaatattc tatactcaac
    10621 gaagttatat tggcagtagt tgactgaacg aaaatacgct tgtaacaagc ttttttcaat
    10681 tctagtcagg ggccccaaca cagaagctga cgaaaagtca gcttacaata atgtgcaagt
    10741 tggggatggg ccccaacaca gagaatttcg aaaagaaatt ctacaggcaa tgcgagttgg
    10801 ggtgggacga cgaaatatat tttgcgaaaa tatcatttct gtcccactcc ctatgcagct
    10861 atcaacttca tatgtatggc gcatacttga taaatgaata tcatcaagat tttaaaagta
    10921 tcgctaattt taaagtctta aaacgcatga ttaatgtcat gatgtgttat tgcgttttgt
    10981 ttttagagcc acgtatttcc taggacatat agaggaacat tcaacgtaaa attcccgaaa
    11041 attaatttta atcattctat aagtgaatgg ttattttcag aagtgattac ttttttacgt
    11101 tacatgagta tttaactttc tatttcaaat tacctagaat tatagcagct atcacgataa
    11161 taataatacc tgcccaaata cccgtcatct gacgacgatc ttttctttct cctaaaatga
    11221 aaataccacc taatgttgaa acgataacaa gtaactgtga taatgagaaa cttgtagcta
    11281 caccaacttt tggttgagaa tagaacatga acaagttacc aattccccaa atcacacctg
    11341 gcaatagatt aagtgctgtt gatttaagtg atgttttatg attcatggat aggataaagc
    11401 caccaattgc cataccgaca gattggaaga acaatgcatc agttccacca acaccaaaga
    11461 tgtcaccaag tacaacgaaa cctacatatc caactgtaga tacaattaaa atacccattg
    11521 cttttttaaa ttcaggatta tctgattgac gttcattttt ggctttcagt gaagtaagtg
    11581 ctacaccagt aactaataat atcatggcga ttaaaccaaa gataatttga gtcattgaag
    11641 accattcacc taaaaagata acgctgaata atgttgtacc aactaattgc atacctgtag
    11701 aaactggcat agtatttgat acaccgatta aactaataga tttaagctga ttcgcttgtc
    11761 caaacgccca taatgcacca gaaataagac cgacaataat tacagtagga ttattgaatt
    11821 ttgcgaaacc agtaataagt aaacctaatc caacaatgag tgcgcctagc gtcgtaccac
    11881 gaatttggtt gtaaggtcca ccgccaacga acacattaat aagaacgaca cttccccaga
    11941 ataaagcagg taaaagtgcg attaagaaat caagaaattg caagactact cactctccta
    12001 tactatttat gcaagtgctc cccttaattg acacttgcat gttattgtaa agcattctgc
    12061 cctaatagta aaaaacaact acttataatt ttcaatattt acaaaaagta gaaaacccca
    12121 caatacatgc taatgatgta ctgtggggta tttgcttggt attttattca gggattgttt
    12181 gatcttttac gttacgtgat tcgaatttga atttaatgta ctcatagttg atatggaaac
    12241 ctagaccatt aaatcgttcg atgagtttat tatttttgtt acgaatttta aaataaactt
    12301 ttctaatatc aaagttatca aaagcatatt gcaatgcata cgcaagcaac tcgaaagcga
    12361 tacctttcaa acggtaatct acatgagagc tgaagtattt aatctcggcg attgactgtt
    12421 gtgaatcaat ttcaaggtat aaataacctt taagtaaacc ttcgctaaca aataaaaaca
    12481 aatgatgatg gtcgtctaaa ctatcaatga tatcttgtgg tgactgtgcc tgatatttaa
    12541 atgtagttgt gtgtaatttg ctgaaagcac gataaaaccc tttgtgataa ggaatgatat
    12601 ttggttggtg catatcttct tctaatcttg tacgggcttc tatgtaatag tcagtgaagt
    12661 tataacttgc ttgaataact ttcattaatg gtttgtattg ttgaatgcct tcttcaaaag
    12721 aaaagttaaa gacggcatcg tcaggttggt tcgaagtcat tgctttaaat agcgtttcaa
    12781 acgtatcgac agataataca taattgtcag ccacgaatgg gcctatgact ttgaatttat
    12841 ttttatcgta tgcaaaagac aatatgattg ctttgattga atcgttttct ttaatcgaaa
    12901 atacaccagg tgatttaatc gcatcggtta ttaatacatc tatttcattg tactgttgag
    12961 gaagtttata taagtatgat gctgattcat aattcgaatg ttcaatgaac gattttattt
    13021 gtgtaatatc atataactga actatgctca tgtcattacc tccgtacttt ttttactttt
    13081 attatatagt atttcaactg aaatgaaagt taatagtgat attaacatgt tacaatacat
    13141 ttaacacatc atttgcaatc attgaattta aatcaaagat tagtggaata gatgaagcac
    13201 gttcgaaata aaagaacgta tgagaaagga taatttatga aatctttaat attagctgaa
    13261 aaaccatcag tcgcaagaga tattgctgat gctttacaaa taaatcagaa gcgtaatggt
    13321 tactttgaaa ataaccaata tattgtcacg tgggcgttag gtcatctagt gacaaatgcg
    13381 acacctgaac aatacgataa aaatttaaag gaatggcgat tagaagacct tccaattata
    13441 cctaaatata tgaaaactgt tgttattggt aaaacaagca aacaatttaa aacagtaaaa
    13501 gcgttaattt tagataataa agtgaaagat attattattg caacagatgc tggacgagaa
    13561 ggtgaactag ttgcaagatt gattttggat aaagttggta acaaaaagcc aatccgtcga
    13621 ttatggacta gctcagttac taaaaaagct attcaacaag gttttaaaaa tttaaaagac
    13681 gggcgtcaat ataacgattt gtattatgca gcgttagcga gaagtgaggc agattggatt
    13741 gttgggatta atgcaacgcg tgcactaaca acaaagtatg atgcacagct atccctggga
    13801 cgtgttcaga caccaacgat tcaattagta aatacacgac aacaagagat taatcagttc
    13861 aaaccacaac aatactatac attatcatta acggtaaaag ggtttgattt tcagctagaa
    13921 tcaaatcagc gatataccaa taaagaaact ttagaacaga tagttaataa tttgaaaaat
    13981 gtcgatggta agattaaatc tgttgctact aaacataaga agtcgtatcc gcaatcactg
    14041 tacaatttaa cagatttaca acaagatatg tatagacgtt ataaaattgg acctaaagaa
    14101 acattgaata cacttcaaag cttatatgag agacataaag tcgtaaccta tccaagaaca
    14161 gattcaaact atttaacaac tgatatggta gatactatga aagaacgtat tcaggcgacg
    14221 atggcaacaa catataaaga ccaagcacgc ccattaatgt ctaaaacatt ttcatcaaaa
    14281 atgtcgatat ttaataatca aaaagtatct gatcaccatg caattattcc tacagaagtg
    14341 agacctgtca tgtcagactt aagtaataga gaattaaagt tatacgatat gattgtcgag
    14401 cgttttttag aagctttaat gcctccgcac gagtatgacg cgataactgt aactttagag
    14461 gttgcagggc acacatttgt tttgaaagag aatgtaacaa ctgttttagg ttttaaatct
    14521 attagacaag gtgaatctat tacagagatg caacagcctt tttcagaagg cgatgaagtg
    14581 aagatttcaa aaacaaacat tagagaacat gaaacaacac ctccagaata ttttaatgaa
    14641 ggttcgttat taaaagcgat ggagaaccct cagaacttta ttcaattgaa ggataaaaaa
    14701 tatgcgcaaa ctttaaaaca aacaggtggt atcggcacag ttgcaacaag ggccgacatt
    14761 atcgataaat tatttaatat gaatgccatt gaatcaagag acggtaaaat taaagtaacg
    14821 tcaaaaggta aacaaatatt agaattagca ccagaagaat taacgtcgcc acttttaact
    14881 gcacaatggg aagaaaaatt acttttaatt gaacgtggta aatatcaggc gaaaacattc
    14941 attaatgaaa tgaaagattt tacgaaagat gttgtaaatg ggattaaaaa tagtgatcgt
    15001 aaatataaac acgataattt aacaaccaca gaatgcccaa cgtgtggtaa attcatgatt
    15061 aaagttaaaa ctaaaaatgg tcagatgctt gtgtgccaag atccatcttg taagacgaaa
    15121 aagaatgtac agcgcaaaac aaatgcaaga tgtccaaact gtaaaaagaa attaacgttg
    15181 tttggtaaag ggaaagaagc ggtatatcgt tgtgtttgtg gacattctga aacgcaagca
    15241 catatggatc agcgtatgaa gtctaaatcc tctggtaaag tatctcgtaa agaaatgaaa
    15301 aagtatatga ataaaaatga aggtttagac aataatccgt ttaaagatgc attaaagaac
    15361 ttgaatttat agataaaatc gaacaaagtt gaatcagaaa aacgaaaagt tcgcttttgg
    15421 tattgttttt tattaagaat gatattaaac tattaaggta ttttaaaaaa aggagcatcc
    15481 attcgtgaaa aactatttcc agttcgataa atatggaaca aactttaaaa gagaaatctt
    15541 aggcggtatc acaactttct tatctatggc ctatatttta gcagttaacc cgcaagtttt
    15601 aagtttagca ggtgttaaag gcgtatcaga agatatgaaa atggaccaag gtgccatttt
    15661 tgtagcgact gcattagcag catttgtagg ctcgctattc atggggctaa tagctaaata
    15721 tccaattgcg ttagcaccag gtatgggatt aaatgcattc tttgcattta ctgttgtgtt
    15781 aaccatgggt attccttggc aagttggttt aacaggcgtt ttattctcag gtatattctt
    15841 tgcgatatta accgtcacag ggtttaggga agttattatt aatgcaattc cttatcaaat
    15901 gaagatggct gtttcagcag gtataggatt attcattaca tttgtaggtt tacaaagttc
    15961 gggtattata gttaaaaatg aatctacatt agtaacattg ggacatttaa ctgatccacc
    16021 agtacttttg gcgatattcg gtatcgttat tacagttatt ttatatgcaa ttaaattgcc
    16081 gggttctatc tttatcggta tgatcatcac agcaatcgtt ggtatgttca ctggattgat
    16141 tcaaatgcca tctggtattg taggtaagat tccaagtatt gaaccaacgt tcggtgctgc
    16201 atttgaagcg tttaaagacc caagtcaatt attaacgata cagttcttaa ttgttatttt
    16261 aacgttctta tttattgatt tctttgatac agctggtaca ttagtagctg ttgctactca
    16321 agcaggtatt atgaaagata ataaattacc aagagctggt agagcattgt tctcagattc
    16381 tttagctacg attgtaggat cagtttttgg aacaactaca acaacttctt atatcgaatc
    16441 aacttcaggt gttgctgtag gtgctagaac aggatttgct agtatcgtaa caggttgttg
    16501 tttcctatta gcgctgttct ttagtccgtt aatcgctgtt gtaacaagtg cagttacgac
    16561 accagcttta gtggtggtag gtgtattaat ggctgcaaac tttgctgaga taaactggaa
    16621 aagttttgaa gttgcagtgc cagcattcat tacaatcatt atgatgcctt tatcatactc
    16681 aattgcaact ggtattgcgt gtggatttat tttctatcca attacaatgc ttatctctaa
    16741 aaaacataaa gaagtacatc cgataatgta tgtattaatg gtactattca ttctgtactt
    16801 catatttgtt catggttaaa tccagacaca aaaaacgact gccaatgatt ggtagtcgtt
    16861 ttttctttga agtaaggata aaataagaag cgaaataaca taaagcaata ttatgaatgt
    16921 ctttaaatat ccgatataaa tatacttctt ttaaaattgt tggacgtgtt tcttctatat
    16981 tatataccta attaaaaagg ttttaagaat tattctctat gtccatcttt gtattgtgtg
    17041 taaatgacac gagcattttt attaggtata taggttggcg ctgataatac aactaggaca
    17101 aatagaatca ttaggaaaaa taaaatccaa tgaatcaaaa ttcctaagaa ggggatgata
    17161 gctaaaaaac tacctactat accaaatatt ggaataatcg ctataggtct aatcgcattt
    17221 tgtttattca ctaataatat aatagtaaga attagataaa gtaatgcatt aaagattaaa
    17281 ggctgccatc caaaacttaa aataaacgtt gcacctaaaa aaggaatgcc atagaaaaac
    17341 tcacaaagta ttaaaaagat acctataagt gctaaagata attttggtcc atttttcata
    17401 ttcattcacc accgttctta tgactaatta tatagaataa ttttcaatga taataataaa
    17461 atgattcaag gtacgaattt acaataaaga aggaaataaa aaatatctcg aaaatagttg
    17521 aactgactaa gtatataaag taaaatataa aagtatgtgt tagacagata gaaaaattat
    17581 gtgaaaagcc ttgatttatc gatgtttttc gtgtaatata actaacgttg gactttaaga
    17641 aaagcgatga agcgaaaggt tactgacaca cccggccgct ttgccatggc gctgtgtaag
    17701 atagttttcg tggagaagtc tatcactaaa tgtagacgaa taaggaggga aaattatggc
    17761 aaaacaaaaa atcagaatca gattaaaagc ttatgatcac cgcgtgattg atcaatcagc
    17821 agagaagatt gtagaaacag cgaaacgttc tggtgcagat gtttctggac caattccgtt
    17881 accaactgag aaatcagttt atacaatcat ccgtgccgtg cataagtata aagattcacg
    17941 tgaacaattc gaacaacgta cacacaaacg tttaatcgat attgtaaacc caacaccaaa
    18001 aacagttgac gctttaatgg gcttaaactt accatctggt gtagacatcg aaatcaaatt
    18061 ataatagaca attttaggag gtggactttc gatgaccaaa ggaatcttag gaagaaaaat
    18121 tgggatgaca caagtattcg gagaaaacgg tgaattaatc cctgtaacag tagtagaagc
    18181 taaagaaaat gttgtattac aaaagaaaac tgtagaagtt gatggataca acgcaatcca
    18241 agttggattt gaagacaaaa aagcatacaa aaaagatgca aaatctaata aatatgctaa
    18301 taaaccagct gaaggtcacg ctaaaaaagc tgacgcagca cctaagcgct tcattcgtga
    18361 attccgcaat gtagacgtgg atgcttacga agtaggtcaa gaagtctcag tagatacttt
    18421 tgtagctggc gacgttattg acgtaacagg cgtatcaaaa ggtaaaggtt tccaaggtgc
    18481 aattaaacgc cacggacaat ctcgtggacc tatgtcacac ggttctcatt tccacagagc
    18541 accaggttct gtaggtatgg cttcagatgc ttctagagta tttaaaggcc aaaaaatgcc
    18601 aggacgtatg ggtggaaaca ctgtaactgt tcaaaactta gaagtagttc aagttgacac
    18661 agaaaacaaa gttatcttag taaaaggtaa cgtacctgga cctaaaaaag gtttagtaga
    18721 aatcagaact tcaattaaaa aaggtaataa ataataagaa gtgaaaggag gaaatgcata
    18781 atggctaatt atgatgtttt aaaattagac ggaactaaat caggttcaat cgaattaagc
    18841 gatgcagtat tcggtattga gccaaataat agcgttttat tcgaagctat taatttacaa
    18901 cgtgcttcat tacgtcaagg tacgcatgct gttaagaatc gttcagcagt aagcggtggc
    18961 ggacgtaaac catggaagca aaaaggaaca ggtcgtgctc gtcaaggtac aatccgtgct
    19021 ccacaatggc gtggcggtgg tatcgtattc ggaccaactc caagaagtta tgcatacaaa
    19081 atgcctaaga aaatgcgtcg tttagcttta cgctcagcat tatctttcaa agctcaagag
    19141 aatggcttaa ctgtagttga cgcattcaac ttcgaagctc caaaaactaa agaattcaaa
    19201 aatgtattat ctacattaga acaacctaaa aaagtattag tagttactga aaacgaagat
    19261 gtaaatgttg aattatcagc acgcaacatc cctggcgttc aagtgacaac tgctcaaggt
    19321 ttaaatgttt tagatatcac taatgctgac agcttagtaa ttactgaagc tgctgctaaa
    19381 aaagttgagg aggtgctcgg ataatggaag caagagatat tcttaagcgc cccgtaatca
    19441 ctgagaaatc ttctgaagca atggctgaag acaaatacac tttcgacgtt gatactcgtg
    19501 ttaacaaaac acaagtaaaa atggctgttg aagaaatctt caacgtaaaa gttgcaagtg
    19561 ttaatatcat gaattacaaa cctaagaaaa aacgtatggg ccgttaccaa ggctatacaa
    19621 acaaaagaag aaaagcgatt gtaactctta aagaaggatc aatcgactta tttaactaat
    19681 aaaacaatta ccattaagga ggtaagcgac aatggctatt aaaaagtata agccaataac
    19741 aaatggtcgt cgtaatatga cttcgttaga tttcgcagaa atcacgaaaa ctacacctga
    19801 aaagtcatta ttaaaaccgc taccgaaaaa agcgggacgt aacaaccaag gtaaattgac
    19861 tgtaagacac catggtggtg gacacaaacg tcaataccgt gttatcgatt tcaaacgtaa
    19921 caaagatggt atcaatgcaa aagttgattc tattcaatat gatccaaacc gctcagcaaa
    19981 catcgcttta gttgtatatg cagacggtga aaaacgatat atcattgctc ctaaaggatt
    20041 agaagtaggt caaatcgttg aaagtggtgc tgaagctgac atcaaagttg gtaacgcatt
    20101 accattacaa aacattccag ttggtacagt agtacacaac atcgagctta aacctggtaa
    20161 aggtggacaa atcgctcgtt cagctggtgc aagtgctcaa gtacttggta aagaaggtaa
    20221 atacgtatta atcagattaa gatctggtga agttcgtatg atcttatcta cttgccgtgc
    20281 tacaatcggt caagttggta acctacaaca cgaattagtt aacgttggta aagccggacg
    20341 ttcaagatgg aaaggtatcc gtccaacagt tcgtggttct gtaatgaacc ctaacgatca
    20401 cccacacggt ggtggtgaag gtcgtgctcc tatcggtaga ccatctccaa tgtcaccatg
    20461 gggtaaacct acgcttggta agaaaactcg tcgtggtaaa aaatcatcag acaaacttat
    20521 cgttcgtgga cgtaagaaaa aataatataa tcaacttatt tgggtgtgcg gcttaaagct
    20581 gcacgcacat aataagaagg gaggcgccca aatggctcgt agtattaaaa aaggaccttt
    20641 cgtcgatgag catttaatga aaaaagttga agctcaagaa ggaagcgaaa agaaacaagt
    20701 aatcaaaaca tggtcacgtc gttctacaat tttccctaat ttcatcggac atacttttgc
    20761 agtatacgac ggacgtaaac acgtacctgt atatgtaact gaagatatgg taggtcataa
    20821 attaggtgag tttgctccta ctcgtacatt caaaggacac gttgcagacg acaagaaaac
    20881 aagaagataa tatctattaa gtagaggagg acatcctaat ggaagcaaaa gcggttgcta
    20941 gaacaataag aatcgcacct cgtaaagtaa gactagttct tgacttaatc agaggtaaaa
    21001 atgctgctga agctattgca attttaaaat taacaaacaa agcttcatca ccagtaattg
    21061 aaaaagtatt aatgtccgct ttagctaatg ctgaacataa ctatgacatg aacacagatg
    21121 aattagtagt taaagaagca tatgctaacg aaggaccaac attaaaacgt ttccgtccac
    21181 gtgcgcaagg tcgtgcaagt gcgattaaca aacgtacaag ccacattaca atcgtcgtaa
    21241 gtgacggtaa agaagaagct aaagaagctt aattaacttt taaggaggga atactgtggg
    21301 tcaaaaaatt aatccaatcg gacttcgtgt tggtattatc cgtgattggg aagctaaatg
    21361 gtatgctgaa aaagacttcg cttcactttt acacgaagat ttaaaaatcc gtaaatttat
    21421 tgataatgaa ttaaaagaag catcagtttc tcacgtagag attgaacgtg ctgcaaaccg
    21481 tatcaacatt gcaattcata ctggtaaacc tggtatggta attggtaaag gcggttcaga
    21541 aatcgaaaaa ttacgcaaca aattaaatgc gttaactgat aaaaaagtac acatcaacgt
    21601 aattgaaatc aaaaaagttg atcttgacgc tcgtttagta gctgaaaaca tcgcacgtca
    21661 attagaaaac cgtgcttcat tccgtcgtgt acaaaaacaa gcaatcacta gagctatgaa
    21721 acttggtgct aaaggtatca aaactcaagt atctggtcgt ttaggcggag ctgacatcgc
    21781 tcgtgctgaa caatattcag aaggaactgt tccacttcat acgttacgtg ctgacatcga
    21841 ttatgcacac gctgaagctg acactactta cggtaaatta ggcgttaaag tatggatcta
    21901 tcgtggagaa gttcttccta ctaagaacac tagtggagga ggaaaataat aatgttacta
    21961 ccaaaacgtg taaaatatcg tcgtcaacat cgtcctaaaa caactggtcg ttctaaaggc
    22021 ggtaactacg taacatttgg tgagtttggt ttacaagcta caacaacgtc ttggatcaca
    22081 tctcgtcaaa tcgaatctgc tcgtatagca atgacacgtt acatgaaacg tggcgggaaa
    22141 gtttggatta aaatcttccc acatacacca tatactaaaa aacctttaga agtacgtatg
    22201 ggtgctggta aaggtgcggt tgaaggctgg atcgcagttg ttaaaccagg tagaatttta
    22261 ttcgaagttg ctggcgtttc tgaagaagtt gcgcgtgaag cactacgttt agcaagtcac
    22321 aaacttccag taaaaactaa gtttgtaaaa cgtgaggaat tgggtggtga aacaaatgaa
    22381 agctaaggaa attagagact taaccacttc agaaatcgaa gaacaaatca aatcttcaaa
    22441 agaagagctt tttaacctac gctttcagtt agctacaggt caattagaag aaactgcacg
    22501 tattcgtaca gtaagaaaaa cgattgcacg tctaaaaact gttgctcgtg aaagagaaat
    22561 tgaacaaagt aaggctaatc aataattaag taagaggagg ttacaaaagt gagcgaaaga
    22621 aacgatcgta aagtttatgt aggtaaagtt gtttcagaca aaatggacaa gactattaca
    22681 gtacttgttg aaacttacaa aacacacaaa ttatacggta aacgagtaaa atactctaaa
    22741 aaatacaaaa ctcatgatga aaacaattca gctaaattag gagacattgt taaaattcaa
    22801 gaaactcgtc ctttatcagc aacaaaacgt tttcgtttag tagagattgt tgaagagtca
    22861 gtaattattt aatacaagtt tagagataag ggaggtttaa ctaatgatcc aacaagaaac
    22921 acgcttgaaa gtagcagaca actctggtgc tcgtgaagtt cttacaatca aagtattagg
    22981 tggatctggt cgtaaaacag caaacatcgg cgatgttatc gtatgtactg ttaaaaatgc
    23041 aacaccaggt ggcgttgtta aaaaaggtga cgttgtcaaa gctgtaatcg tacgtactaa
    23101 gtcaggtgtt cgtcgtaatg acggttcata catcaaattt gatgaaaatg catgtgttat
    23161 catccgtgat gacaaaggcc cacgtggtac tcgtatcttc ggacctgttg ctcgtgaatt
    23221 acgtgaaggt aacttcatga aaatcgtatc attagcacca gaagtacttt aattaataac
    23281 aaccaataca aataaggagg tgcccacatg catatcaaaa aaggtgacaa cgttaaagtt
    23341 atcgcaggta aagacaaagg taaagaaggt aaagtaattg ctactctacc taaaaaagac
    23401 cgtgtcgttg tggaaggtgt taacattatg aaaaaacacc aaaaaccaac tcaattaaat
    23461 cctgaaggtg gaatcttaga aacagaggca gcaatccatg tttctaatgt acaattattg
    23521 gaccctaaaa caaacgaacc aactcgtgta ggttacaaat ttgttgatgg taaaaaagtt
    23581 cgtatcgcta aaaaatctgg cgaagaaatt aaatctaata attaataaca agttgaaagg
    23641 aggatcccac tttgaaccgt ttaaaagaaa agtttaacac tgaagttact gaaaacttaa
    23701 tgaaaaaatt caattatagt tcagtaatgg aagtaccaaa aatagataaa atcgttgtga
    23761 acatgggtgt aggtgacgca gtacaaaatt ctaaagtatt agacaatgct gttgaagaat
    23821 tagaattgat cactggtcaa aaaccattag taactaaagc taaaaaatca atcgcgactt
    23881 tccgtttacg tgaaggtatg ccaatcggtg cgaaagtaac acttcgcggt gaaagaatgt
    23941 atgaattctt agacaaatta atttcagtat cattaccacg tgtacgtgac ttccaaggtg
    24001 tttctaaaaa agcatttgac ggacgcggta actacacttt aggtgttaaa gaacaattaa
    24061 ttttcccaga aatcgactat gataaagtaa gtaaagttag aggaatggat attgttatcg
    24121 taacgactgc taacactgat gaagaagctc gtgaattgtt agctaacttc ggtatgccat
    24181 tccgtaaata attatttaaa ggaggctaat taagtggcta aaacttcaat ggttgctaag
    24241 caacaaaaaa aacaaaaata tgcagttcgt gaatacactc gttgtgaacg ttgtggccgt
    24301 ccacattctg tatatcgtaa atttaaatta tgccgtattt gtttccgtga attagcttac
    24361 aaaggccaaa tccctggcgt tcgtaaagct agctggtaat aaaaaagagt ctgaaaggag
    24421 gcaacaatca atgacaatga cagatccaat cgcagatatg cttactcgtg taagaaacgc
    24481 aaacatggtg cgtcacgaga agttagaatt acctgcatca aatattaaaa aagaaattgc
    24541 tgaaatctta aagagtgaag gtttcattaa aaatgttgaa tacgtagaag atgataaaca
    24601 aggtgtactt cgtttattct taaaatatgg tcaaaacgat gagcgtgtta tcacaggatt
    24661 aaaacgtatt tcaaaaccag gtttacgtgt ttatgcaaaa gctagcgaaa tgcctaaagt
    24721 attaaatggt ttaggtattg cattagtatc aacttctgaa ggtgtaatca ctgacaaaga
    24781 agcaagaaaa cgtaatgttg gtggagaaat tatcgcatac gtttggtaat aaaaaataag
    24841 gaggtgccat aacatgagtc gtgttggtaa gaaaattatt gacatcccta gtgacgtaac
    24901 agtaactttt gatggaaatc atgtaactgt taaaggtcct aaaggtgaat tatcaagaac
    24961 tttaaatgaa agaatgacat tcaaacaaga agaaaacaca attgaagttg taagaccatc
    25021 tgattctaaa gaagatagaa caaaccatgg tacaactcgt gctttattaa acaatatggt
    25081 acaaggtgtt tctcaaggat acgtaaaagt acttgaactt gttggtgtag gttaccgtgc
    25141 tcaaatgcaa ggtaaagact taatccttaa cgttggttat tctcacccag tagaaattaa
    25201 agctgaagaa aacattactt tctcagttga gaaaaacaca gtcgttaaag ttgaaggtat
    25261 ttcaaaagaa caagttggag cattagcatc taacatccgt tcagtaagac ctccagagcc
    25321 ttacaaaggt aaaggtattc gttaccaagg tgaatacgtt cgccgtaaag aaggtaaaac
    25381 tggtaaataa tagataaact ctaaagaaag gagtattgaa atgatcagta aaattgataa
    25441 aaataaagtg cgtttaaaaa gacatgctcg tgttcgtact aacttatcag gtacagctga
    25501 aaagccacgt ttaaacgtat atcgttcaaa caagcatatc tacgctcaaa ttattgatga
    25561 taataaaggc gtaacattag ctcaagcttc ttcaaaagac agcgacattg ctactacagc
    25621 aactaaagtt gaattagcaa ctaaagtcgg tgaagcaatt gctaaaaaag ctgctgacaa
    25681 aggcattaaa gaaatcgtat ttgaccgtgg aggatattta tatcacggac gtgttaaagc
    25741 attagctgaa gcagcaagag aaagcggatt agaattttaa tttaaaggag ggacaaatac
    25801 atggctcgta gagaagaaga gacgaaagaa tttgaagaac gcgttgttac aatcaaccgt
    25861 gtagcaaaag ttgtaaaagg tggtcgtcgt ttccgtttca ctgcattagt tgtagttgga
    25921 gacaaaaatg gtcgtgtagg tttcggtact ggtaaagctc aagaggtacc agaagcaatc
    25981 aaaaaagctg ttgaagcagc taaaaaagat ttagtagttg ttccacgtgt tgaaggtaca
    26041 actccacaca caattactgg ccgttacggt tcaggaagcg tatttatgaa accggctgca
    26101 cctggtacag gagttatcgc tggtggtcct gttcgtgccg tacttgaatt agcaggtatc
    26161 actgatatct taagtaaatc attaggatca aacacaccaa tcaacatggt tcgtgctaca
    26221 atcgatggtt tacaaaacct taaaaatgct gaagatgttg cgaaattacg tggcaaaaca
    26281 gtagaagaat tatacaatta aggagggaaa actagttatg gctaaattac aaattaccct
    26341 cactcgtagt gttattggtc gtcctgaaac acaacgtaaa actgttgaag ctttaggtct
    26401 taaaaagact aacagttcag tagttgttga agataaccct gctattcgtg ggcaaatcaa
    26461 caaagttaag cacttagtaa cagtagaaga aaaataatca aggaggtgcc gaaatgaaat
    26521 tacatgagtt aaaaccggca gaaggttcac gtaaagaacg caatcgtgtt ggacgtggtg
    26581 ttgcgacagg taatggtaaa acaagtggtc gcggacacaa aggtcaaaaa gctcgttcag
    26641 gcggtggtgt aagaccagga tttgaaggtg gtcaattacc attattccgt cgtttaccaa
    26701 aacgtggttt tactaacata aatcgtaaag aatatgctat tgttaactta gaccaactta
    26761 ataaatttga agatggtact gaagtaactc cagctttatt agtagaatct ggtgttgtta
    26821 agaatgaaaa atctggtatc aaaatactag gtaatggttc acttgataag aaattgacag
    26881 tgaaagctca taaattctca gcttcagcag cagaagctat tgatgctaaa ggtggagcac
    26941 acgaggtgat ctaatgattc aaacccttgt gaacttcttt agaacaaaag aggttcgtaa
    27001 caagattttc ttcacactag caatgttagt aatttttaaa atagggactt atataccagc
    27061 tccaggagta aatcctgcag cttttgataa tccccaaggt tctcaaggtg ccactgagtt
    27121 attaaatact tttggtggcg gagccttgaa acgattttct atttttgcaa tgggtattgt
    27181 accctacatc actgcatcaa tcgtaatgca attattacaa atggatattg tccctaaatt
    27241 ctcagaatgg gcaaaacaag gtgaagtagg tagaagaaag ttaaataacg ttactcgtta
    27301 tttagcaatt tctttagcat ttatccaatc tataggtatg gcattccaat ttaataatta
    27361 tctcaaaggt gcgctgatta tcaatcagtc aattatgagt tatttattaa tagcactagt
    27421 tttgacagca ggaactgctt tcttaatatg gcttggtgat caaatcactc agttcggtgt
    27481 tggtaatggt atttctatta tcatatttgc gggtatttta tcaacgttgc cagccagtct
    27541 aattcaattt ggacaaactg cgttcgttgg gcaagaagat acatcattag catggttgaa
    27601 agtattagga cttttagtgt cactgatact attaacagta ggtgctattt atgtacttga
    27661 agccgttcgt aaaattccga ttcaatatgc taagaaacaa actgctcaaa gattaggttc
    27721 acaggcaact tatctacctt taaaagtgaa ctcagccggt gttattcctg taatctttgc
    27781 aatggcgttc ttcttactgc ctagaacatt aacgttgttc tatccagata aagaatgggc
    27841 acaaaacatt gcgaatgctg caaatccttc aagtaatgtt ggtatggttg tatatattgt
    27901 actaattata ctattcacat acttctatgc atttgtacaa gttaatccgg aaaaaatggc
    27961 tgataacctt aagaaacaag gtagctatgt tccaggtatt agacctggtg aacaaaccaa
    28021 aaaatatatt actaaagttt tatatcgttt aacttttgta ggttctatct tcttagccgt
    28081 tatttcaatt ctaccgatat tagcaactaa gtttatggga ttaccacaat caattcagat
    28141 tggtggtaca agtttactta tcgttattgg tgtagcgatt gaaacaatga aatctttaga
    28201 agcacaagta tctcaaaaag aatataaagg ctttggtggt agataatttt aggagggcac
    28261 ttatgaatat cattttgatg ggtttacctg gcgcaggtaa aggaactcaa gcaagtgaaa
    28321 ttgtcaagaa attcccaata ccccacattt caactggtga catgttcaga aaagctataa
    28381 aagaagaaac tgaattaggt aaagaagcta agtcttatat ggaccgtggc gaattagttc
    28441 ctgatgaagt gactgtaggt atcgttaagg aaagaatttc tgaagacgat gcaaaaaaag
    28501 gctttttatt agatggcttc ccaagaacaa tcgagcaagc tgaggcatta aataatatta
    28561 tgtctgagct tgacagaaac attgatgctg tcatcaatat cgaagttccg gaagaagaat
    28621 taatgaaccg tcttacaggt cgtcgaatct gtgagtcatg tggtacaacg tatcatcttg
    28681 tatttaatcc tccgaaggtc gaaggtattt gtgatatcga tggtggtaaa ttgtatcaac
    28741 gagaagatga taatcctgaa acggtagcta atcgtttgag tgttaatatt aaacaatcta
    28801 agcctatttt agatttctat gatcaaaaag gtgtattgaa aaatattgat ggttcaaaag
    28861 atattagcga cgttaccaaa gatgtcattg atattttaga tcatttgtaa tagatcaact
    28921 tttagcggtc tattttaagt gaggcatgat ttaatcatat tagatagatg agagtggcaa
    28981 ttaacgataa ttaattcccg taatttttgt ttaacttata aaattaatca ctgatagatt
    29041 cgtaacaata aagttgtcta ttgacgatta ccttactatt gcaaaaaggg ggaagttaat
    29101 caatggctaa acaagatgta attgaattag aaggtactgt attagatact ttaccgaacg
    29161 caatgtttaa agtagaatta gaaaatggtc atgagatttt agctcacgta agtggtaaaa
    29221 tcagaatgaa ttacattcgt attctacctg gcgacaaagt aactgttgag atgtctccgt
    29281 acgatttaac acgcggaaga attacttatc gttataaata atcgtcactc cataatatag
    29341 ggaggtataa aaatgaaagt aagaccatca gtaaaaccta tttgcgaaaa atgtaaagtc
    29401 attaaacgta aaggtaaagt aatggtaatt tgtgaaaatc caaaacacaa acaaagacaa
    29461 ggttaataaa agagaggtgt aaattaatat ggcacgtatt gcaggagtag atattccacg
    29521 tgaaaaacgc gtagttatct cattaactta tatatacggt atcggtacgt caactgctca
    29581 aaaaattctt gaagaagcta acgtatcagc tgatactcgt gtgaaagatt taactgatga
    29641 cgaattaggt cgcatccgtg aagttgtaga cggttataaa gtcgaaggtg acttacgtcg
    29701 tgaaactaac ttaaatatca aacgtttaat ggaaatttca tcataccgtg gtatccgtca
    29761 ccgtcgtggt ttaccagttc gtggtcaaaa aacgaaaaac aacgcgcgta ctcgtaaagg
    29821 accagttaaa acggtagcta acaagaaaaa ataataggta aaggaggcaa attttaaatg
    29881 gcacgtaaac aagtatctcg taaacgtaga gtgaaaaaga atattgaaaa tggtgtagca
    29941 cacatccgtt caacattcaa caacactatt gtaactatca ctgatgagtt cggtaatgct
    30001 ttatcatggt catcagctgg tgcattagga ttcaaaggat ctaaaaaatc aacaccattt
    30061 gcagcacaaa tggcttctga aactgcatct aaatcagcta tggagcatgg tttaaaaaca
    30121 gttgaagtaa cagttaaagg acctggtcca ggtcgtgaat cagctattcg tgcattacaa
    30181 tctgcaggtt tagaagtaac tgcgatcaga gacgttactc cagtacctca taacggttgt
    30241 cgtccaccaa aacgtcgtcg tgtataattt atgatggtat tgttacaggt cactgagcaa
    30301 acattttaaa ttaagtcgac gtatataagg aggatattta aatgatagaa atcgaaaaac
    30361 ctagaattga gacaattgaa attagtgaag atgctaaatt cggtaagttc gttgttgaac
    30421 cactagaacg tggctacggt actacactag gaaactcctt acgtcgtatc ctactatctt
    30481 cattaccagg tgcagccgtt aagtatattg aaattgaggg agttttacat gaattctcag
    30541 cagtagacaa tgtagttgaa gatgtttcta caattattat gaacattaaa caattagcat
    30601 tgaaaattta ctctgaagaa gataaaactt tagaaattga tgtacgtgat gaaggcgaag
    30661 taacagcaag cgacattaca catgatagtg atgttgaaat tttaaaccca gagcttaaaa
    30721 ttgcaacagt atctaaaggt ggtcacttaa aaattcgtct agttgctaac aagggtagag
    30781 gttacgcatt agcagaacaa aataatacta gtgatttacc aattggtgta atccctgttg
    30841 attcattgta ttcacctgtt gaacgtgtga actatactgt tgaaaataca cgtgtaggtc
    30901 aaagcagtga ttttgataaa ttaacattgg atgtttggac taatggttca atcacaccac
    30961 aagaatcagt ttcattagca gcaaaaataa tgactgaaca cttgaatatc ttcgttggtc
    31021 ttactgatga agcgcaaaac gctgaaatca tgattgaaaa agaagaagat caaaaagaaa
    31081 aagtattaga aatgtctatt gaagaattag acttatctgt acgttcatat aactgcttaa
    31141 aacgcgcagg aatcaattct gttcaagagt tagctgacaa atctgaagct gacatgatga
    31201 aagtgcgtaa tttaggtcgt aaatctttag aagaagttaa atacaaatta gaagatttag
    31261 gattaggatt aagaaaagaa gattgataaa ggaggttaac tcatgggtta cagaaaatta
    31321 ggtcgtactt ctgatcaacg taaagctatg ttacgtgact tagctacatc acttattatt
    31381 agtgaacgta ttgaaactac agaagctcgt gcaaaagaag ttcgcagtgt tgttgagaaa
    31441 ttaatcactt taggtaaaaa aggagattta gcttctcgtc gtaatgcagc taaaacttta
    31501 cgtaatgttg aaatcttaaa cgaagatgaa actacacaaa ctgcacttca aaaattattt
    31561 ggtgaaatcg cagagcgtta cacagaacgt caaggtggtt acactcgtat ccttaaacaa
    31621 ggccctcgtc gcggtgacgg tgctgaatca gtaattatcg aattagtata agaatttata
    31681 taaatacact aactactttt atataaagca aatgagtgca acgataatgt ttgccacata
    31741 caaatattgt ctagctcaga gtaccccatc aactaaataa agatttaaag cgtgaactca
    31801 acaaacttat gatggggtgc gcgctttttt attgaataag atatcggact tatgaacaga
    31861 aatgttttta agtccgattt ctttatattt acttttttga gtgcgaatct aaaaatatga
    31921 tgaagctagt ttgatcaatt tcactagaac tgaatgaagt gaaagtatta tcgttgaatc
    31981 aaacgcgacc acccctatat tagtaaatca tttattttat taacgtttat acataattgt
    32041 tagagaggaa taaaagtcct tatcattagt tgttaaacaa cactttaaag actagaaatg
    32101 agtcagaaaa tactagtaat atatagagaa cccagtcaat gtcatataca gcgatttata
    32161 aatattgtaa aatatgatag atacacgaat cgtaaaggag agagacaagt ggaggataag
    32221 aattcagtta ttgtatttaa aaatgtttca tttcaatatc aaagtgatgc atccttcaca
    32281 ttgaaagatg tttcttttaa tatacctaaa ggtcagtgga catctattgt tggtcataac
    32341 ggttctggaa aatctacaat tgccaagtta atgattggca tagagaaagt taaatctgga
    32401 gaaatttttt ataataatca agctataact gatgataatt ttgaaaagtt aagaaaagac
    32461 ataggaattg tatttcagaa tccggataat caatttgttg gttcaattgt aaaatacgat
    32521 gtggcatttg gactcgaaaa tcatgcggtt ccacatgacg aaatgcatag aagagttagc
    32581 gaagcactta aacaagttga tatgttagaa cgtgcagatt atgaacctaa tgcattatcg
    32641 gggggacaga agcagcgtgt ggctatagca agtgtattag cacttaaccc ctctgtcatt
    32701 atattagatg aggcgacttc tatgttagat cctgatgcac gtcaaaattt attggattta
    32761 gtgagaaaag ttaaatcaga acataatatt acaatcattt ctattacgca tgatttatct
    32821 gaggcgatgg aagcagatca tgttatcgtt atgaataaag ggactgtcca taaagaaggc
    32881 acagcgattg aaattttcga ccatgcagaa gagttaacaa caataggtct agatttgcca
    32941 ttcccaatca aaataaatca aatgctggga caccaaacat cattcttaac ttatgaaggg
    33001 ctggtggatc aactatgact atacgctttg acaatgtaag ttatacctat caaaaaggga
    33061 caccatacca gcatcaagct attcatgatg ttaatacaga atttgaacaa ggtaaatatt
    33121 acgccatcgt tggacaaacg ggtagtggta aatcaacatt gatacaaaat attaatgcac
    33181 tgttaaagcc gactactggg acagttacag ttgatgacat tactattaca cataagacca
    33241 aagataaata tattagacct gtaagaaaaa gaattggaat ggtatttcaa ttccccgaat
    33301 ctcaattatt tgaggacaca gtagagcgtg aaatgatatt tggacctaaa aactttaaaa
    33361 tgaatttaga tgaagccaaa aactatgccc atcgtctgtt gatggattta ggtttttcaa
    33421 gagatgtaat gtcacaatca ccatttcaaa tgtcaggtgg acaaatgcgt aaaatagcga
    33481 ttgtatcgat attggcaatg aatcctgata ttatcgtggt tgatgaaccc acagcaggac
    33541 ttgatccaca aagtaaacga caagtaatgc gattactaaa gtcactacaa acagatgaaa
    33601 ataaggcaat tatcctaatt tcacatgata tgaatgaagt cgcgcgttat gcagatgaag
    33661 tcatcgttat gaaagaaggt agtatagtgt cgcaaacatc accaaaagag ctcttcaaag
    33721 ataaagaaaa attggcggat tggcatattg gtttgccaga aattgttcag ttacaatatg
    33781 actttgaaca aaaatatcaa acaaaattaa aagatattgc cttaactgaa gaagcatttg
    33841 taagcttgta taaggagtgg caacatgaaa aataaattga ttatagggcg atatttacca
    33901 attaattctt ttgttcatca tcttgatcca agagcaaagt taatgtttgt ttttcttttt
    33961 attatattaa tatttttctg tcattcacca ttaacatatc tttgggtgtt tgcacttatc
    34021 ttattcatta tgagattagc aaaaattcaa ttatggttcc taatcaaagg tttaacacca
    34081 atatttttct ttttaatctt cacattaatg atgcatatat ttttaactaa aggtggatat
    34141 gtattagttg aatggcatgg catcacgatt gaaactaatg gtattttaga agggctatat
    34201 atatcactgc gtttaattgg gattgtgatg attgcaacaa ttatgacact atctacaagt
    34261 ccaattgatt taacagatgc gtttgaaaga ttacttgcac cactaaagat gtttaaatta
    34321 ccagttcatc aattaagtat gataatgtcc attgcgttac gattcatccc gacgttaatg
    34381 gatgagttag ataaaattat tttggcgcaa aagtcgcgtg gttctgaaat aagttcaggg
    34441 aacattgcaa cacgtattaa atcatttatt ccattactag tgccactatt catctcagct
    34501 ttccaacgcg ccgaagaatt agcggtcgca atggaagtta ggggttatga tgccaatgtt
    34561 aagagaacga gctacagaca gcttaaatgg caattgagag atacgttatc tttaatcatg
    34621 attatcccaa ttgcaattat tttattcgta ttaaaatatt caggagtgta aattcatgcg
    34681 tatattagta gaaattgcgt atcaaggaaa taattttcta ggctttcaaa ttcaacagaa
    34741 tggacgtacg gtacagcaac aatttgaaaa gctattacaa cgcatgcata aaagacatgt
    34801 gagaatccat ccttcaagta ggactgatag aggtgtacat gcgatacagc aatactttca
    34861 tttcgacaca gagctgaata tacctatgtc acaatggcaa tatgcaatga accgaacgtt
    34921 acctgatgat atttatgtca acaatgtagt tacagtcgat gacgattttc attgtcgtta
    34981 cgattgtgta ggtaaacgct atcgatataa agtatatcaa gcacaacacc gcgatccatt
    35041 tcaaagtggt ttgaaaacat ttattcctga aacgttggat ttagataaaa tgaacagagc
    35101 ggcacaacaa ttcataggta cacatgattt tacgggattt tgttcgcaaa aaactgaagt
    35161 agaaagcaag gttagaacat tataccaaag tgaaattgta aagacagatg atggctttga
    35221 ttatattgtc actggatctg gatttttata taacatggtg cgtgtcttag ttgcattttt
    35281 aatagaagtt ggaaaaggac gacatgaaat ttctgatgtg ccgaagctac ttgaatcaaa
    35341 gaatcgtaag aatgttcctt tcactgcaac ggctgaagga ttatatttag agaagattta
    35401 tttagatgaa aatgagttat taaaagactt tggcaacgat atcaaaatac atcgtaaaaa
    35461 atcattacaa aatgactgat tgccattgac aaaaagcttg tgaaattata agattattaa
    35521 cggtattgtt ttatatccac cccacgataa gccccggaaa cttattgtgt tacaagatat
    35581 ataagcagaa acgaacaaca gttaacaaaa taaatgaaat taaacgtttt aaaaatgaaa
    35641 caaatgaaat catctattag gttatgaaac tgtttatagc ttgaatagaa gcatttattt
    35701 tttaggagga caattattat gcgtcaaaca tttatggcaa atgaatcaaa cattgagcgc
    35761 aaatggtatg ttatcgatgc tgaaggccaa acattaggtc gtttatcatc agaagtagca
    35821 tctatcttac gcggtaaaaa taaagtaact tacacaccac acgttgatac tggtgattat
    35881 gtaatcgtta ttaatgcatc aaaaatcgaa tttactggta acaaagaaac tgacaaagtt
    35941 tactaccgtc actcaaatca cccaggtggt atcaaatcaa tcactgctgg tgaattaaga
    36001 agaactaacc cagaacgttt aattgaaaac tcaattaaag gtatgttacc aagcactcgt
    36061 ttaggcgaaa aacaaggtaa aaaattattt gtatatggtg gcgctgaaca tccacacgct
    36121 gcacaacaac cagaaaacta cgaattacgt ggttaattag aaggaggaaa tgactttggc
    36181 acaagttgaa tatagaggca caggccgtcg taaaaactca gtagcacgtg tacgtttagt
    36241 accaggtgaa ggtaacatca cagttaataa ccgtgacgta cgcgaatact taccattcga
    36301 atcattaatt ttagacttaa accaaccatt tgatgtaact gaaactaaag gtaactatga
    36361 tgttttagtt aacgttcatg gtggtggttt cactggacaa gctcaagcta tccgtcacgg
    36421 aatcgctcgt gcattattag aagcagatcc tgaatacaga ggttctttaa aacgcgctgg
    36481 attacttact cgtgacccac gtatgaaaga acgtaaaaaa ccaggtctta aagcagctcg
    36541 tcgttcacct caattctcaa aacgttaatt gtcggacgat atatacaaaa cacttcgatg
    36601 ttatgtcgag gtgttttttt gcgtttgtgt ggggaatatg aaatgtatgt agtataaatg
    36661 aactttcctt tatttaatgt ctcaggaata gaataaattc aactatgaat tattgattta
    36721 cacgtataat ttgtacatgc ttacacaatc aaaagtttac attattaaat tgtcatttag
    36781 cttgcggagg gtagacgata actcaagagc cttaattaaa aagtattatt aaaataagga
    36841 gtgacttaat tgaaagattc tatattttgg aagaaagctt ttattcctgt ttattttatt
    36901 gttgcgatgc tggtgtttct actttttagg ttttatatta aaacagataa cttttctatg
    36961 tatttaatga ttatcttctt aatttgttta ggaactgcat ctatcattta taactataaa
    37021 accaatcgat aaatataagt atgaaatgta taagtcacag ttaaatctaa atcttgctgc
    37081 ggctttttat cgaaatgaca attttaaatc gccaaaaaca acattttcag tccgtcataa
    37141 aacagcattt tcagcccacc atcgactaaa cacctcatcc caaaatatgc taacaatcct
    37201 ccacaacaat caatccaaca tccctcataa tcacaacgca caaaatctac tcatgcattt
    37261 ttggaatact tagtattaca aataacgatt tttattcatc taacaaaggt tatataatgt
    37321 aatgaaggca atttttatgt attacaaatc taatcgtaca tgtaaaattt tgataaacat
    37381 cattaatttt gcgtaactat cattagatta caaatcacaa tgtaattaca tgtaatacac
    37441 atctatacat cacatttgaa gggaaatgaa tataaatgac tgataaaaag tacactgcag
    37501 ccgatatggt tattgatact ttgaaaaata atggggtaga atatgttttt ggtattccgg
    37561 gtgcaaagat tgactatcta tttaatgctt taattgatga tggtcctgaa cttattgtca
    37621 ctcgtcatga acaaaacgcc gcaatgatgg cgcaaggtat tggaagatta acaggcaaac
    37681 cgggtgtagt acttgttaca agtggccctg gcgtaagtaa tttaacgact ggactattaa
    37741 cagctacatc tgaaggggat cctgtcttag cgttaggcgg ccaagtgaaa cgtaatgatt
    37801 tattacgatt aacgcatcaa agtattgata atgctgcttt attaaaatat tcatcaaaat
    37861 atagtgaaga agtacaagat cctgaatcat tatcagaagt tatgacaaat gcaattcgaa
    37921 ttgctacttc aggaaaaaat ggcgcaagtt ttattagtat tccgcaagac gttatttctt
    37981 caccagttga atctaaagcg atatcacttt gccaaaaacc aagtttaggg gtaccgagtg
    38041 aacaggatat taatgatgtc attgaagcga ttaaaaatgc atcatttcct gttttattag
    38101 ctggtatgag aagttctagt gcagaagaaa caaatgccat tcgtaagcta gtagagcgta
    38161 ccaatttacc cgttgtagaa acattccaag gtgcgggtgt gattagtcgt gaattagaaa
    38221 atcatttctt tgggcgagtg ggcttattcc gcaatcaagt tggtgatgaa ttattacgta
    38281 aaagtgattt agttgttaca atcggttatg atcctataga atatgaggct agtaactgga
    38341 ataaagaatt agatacgcaa ataatcaata ttgacgaagt tcaagctgaa attactaatt
    38401 atatgcaacc gaaaaaagag ttgattggta atattgctaa aacaattgaa atgatttctg
    38461 tcaaagtaga tgagccattt ataaatcaac aacatttaga cgaattagaa caattaagaa
    38521 cacatattga tgaagaaaca ggtatcaaag cgacgcatga agaaggtatt ctacatccag
    38581 tggaaattat tgaatctatg caaaaggtat taactgatga tactactgta acagttgatg
    38641 ttggaagtca ctatatttgg atggcacgta atttcagaag ttataatcca agacatttat
    38701 tatttagtaa cggcatgcaa acgcttggtg tagcattacc gtgggcaatt tcagctgcac
    38761 ttgtacgccc taatacgcaa gttgtatccg ttgctggcga tggtggcttt ttattttcat
    38821 cacaagattt agaaacggcc gtacgtaaaa atctaaacat tattcaactt atttggaacg
    38881 atggaaaata taacatggtt gaattccaag aagaaatgaa atataaacgt tcatcaggtg
    38941 tcgacttcgg tcctgtagat ttcgtaaaat atgctgaatc atttggcgca aaaggtttac
    39001 gagttactaa tcaagaagaa ttagaagcgg caattaaaga gggctatgaa acagatggtc
    39061 cagtattaat tgatatacct gtaaattaca aagataatat caaactttca acaaatatgt
    39121 tacctgacgt atttaactaa aacaaagata aatgttaatg aggagtggga aattttatgg
    39181 ctagtgtttt atatcaacat ggtacattag gcacattaat ggcaggttta ttaaaaggaa
    39241 ctgcatcaat aaatgaatta ttgcaacatg gtgatttagg tatcgctaca ctaacaggtt
    39301 caaacggtga ggtaatcttt ttagatggaa aagcttacca tgcaaatgaa cataaagaat
    39361 ttgtagaatt aaaaggtgat gagttaacac catatgcaac tgtaactaaa ttcgtagcag
    39421 atacaagcta tgaaacgaaa gataaatctt ctgaagcagt ttttgcggaa attaaggaga
    39481 aaatgctgag tgaaaattta ttttcagcag taaaaatttc aggcttattt aaaaaaatgc
    39541 atgtacgtat gatgccggct caagaaccac cttatacacg tttaatcgat tcagctagaa
    39601 gacaacctga acaaactgaa acgtatgtca aaggttcagt cgttggtttc tttacaccag
    39661 aattattcca tggtatcggg tcagcaggat tccatgtaca ctttgcgaat gatgatcgta
    39721 actttggtgg acatgtctta gattttgaag tagaagatgt taaagtagaa atccaaaata
    39781 ttgaaacatt tgaacagcat tttccaattc atgatgaaga tttcactaat gcaaatattg
    39841 actataaaga tattgcagac gaaattagag aagctgagta atgaatgcag aaatataatg
    39901 acggtttatg aaaattgact tcataatgcg cgatttagaa atggtagttt gttaaatatg
    39961 gttaagcatg attacaatag aacaaatata tttataatta cgtctaagta ataaaataaa
    40021 tcccttcact attagcggta gtgaggggat ttatttaggt tccagatatt tgagatttgc
    40081 tgttatgttt agattataaa ttgtggtaga cattaatatc gaatttacaa ttgcataggc
    40141 caatctaatc tattgtcagt agaagcaatc aataataaat tacaagttca atcattcatt
    40201 tcatactaaa aagacaaacg caatttttca gcgtttgtcc tgtataacta ttttcatttt
    40261 caaattatta tttaactaca atttgtattt gtttgattgt acttgaatca attgaatttt
    40321 taacgtcgtc atttttcttt agattcacta cttttttatt accatcattc aaagtaagtg
    40381 tatacgttgc tatttgtgca ttattaattt tttctgttgt aacaccacgt tgagaagcta
    40441 attcattttt tattttactg tcaatttctt gataaagtac ctttttattt tctggaaaaa
    40501 caaagtaagt tcgatgtaat gcagtaatac catctactga gatcgtgtaa ggtacagtgt
    40561 gttttccatc cattgtcaat tccttataac cgttattact atctgcagat gcttcatgac
    40621 tcggtaaagc tgcacctact gttgataata atcccaatgc taaagtggca gtaacaaatg
    40681 attttagttt cataactcaa tctccttttt gttttgtagg atattacact atttgtataa
    40741 cacgtttcat aaagtttaaa acatactttt tgtttttttt atatttttga tttttagtaa
    40801 tagtttttaa cgagggatat gacccgtgta ttttgaaaaa agaattataa taaaccatca
    40861 tgttactata attttaaatc attaattatc atctttattt aatgtttgtt gtaattcaaa
    40921 gcgcacgccg gattcattag aagtcgacgt atttttgttt gtaatagagt atccagtcat
    40981 tgaaatttta gattcaatat ctgaagcggt atttgtagat tcgggattat agaagctaca
    41041 ttcataagtg ttatcatctt tgtttttaag aataaacatt cctttggctt taacttcgac
    41101 tttagtgttg ttaatgtcaa aagtttgagt gctattacta taattgacac cagcccaaac
    41161 cgattcatta tctttcacaa tgtggaagtc atcatcttgt ttaacgatag ttacttggct
    41221 tgctttggat ttaaaattat ctttagataa gcctggatac aagacatatg catatttatc
    41281 atctgtatta gaatgctttt gagttacttc ataatactca tcagttttgt catctgactt
    41341 ttgactttta tttatatcac tccacttacc agtatgactt tcttttttta cagttatttt
    41401 cgattcgttt aaaaaatgat aaccgatgtt cttttttgta tctgtggact ctaaaaagac
    41461 tgaattggtt tcctgatcat taatatttga agcggttgtt tgtttatcgt ctgtaaataa
    41521 cgtataccca ttcgatttgc gattttcaat cgttgtcact ggattctttg atgaatcagt
    41581 acttttaatg ccagttccta agaagacaat tttatcgttt aatatgaaat atgatttttt
    41641 ggcagttaaa gttttgtcct gattttcaaa atccattccg atactagcat gttggtcatc
    41701 gaattttgtt ccgcctacaa aagttttatc ggagttctta ttttctttag gttcttcatt
    41761 atctaaagtt gtagtacctg ctaaacgttt catatcagct gtcgcccaga agttatcacg
    41821 gtagtgtttc acatcgctat tgtataaata agacattcca gcaccagtgt gccaaccttt
    41881 taagttctct ccgttgatac tttcgtaatg tgcgacgttt ttcgacgtca tacttaagcc
    41941 aaatgcaaag tctaagtctt tgttatgata ggtgacacga ttcatgtcat tatatatttt
    42001 aagttgttgt gttaaaccgt tagtagaaat agtgctgtct tcaattaaag acttcatttt
    42061 gcttatatct gaataagagc ttaaataatc gttttgttta taacttgaat cagatttaac
    42121 agaagtttta acgatttgct tatatttagc ttttgttgac tcatccatgg catcacttaa
    42181 tctcaacaat gatttcatta cagttgcaga tgttgagtga ctcgtttcat tttcacggct
    42241 aatggctcta ccacgtgata aatccatcat ttcaccttta taaatgagtg gcataaatcc
    42301 ttcatcaatc cacgacttta atgttgtatc attttgatta ctatctttaa aaggtgtttc
    42361 ttttatcatc ggcatcattt gagaaatccc ctctaagagt acaacgccat aagcaccagt
    42421 gtatgggacg tcttgatgat caatataaga gccgtcttta tagaatccat tacgttcttt
    42481 accagttgca ttactttgaa cgtaagtgaa gactttatta aatgcctcta ttgattcttt
    42541 catcatagtt gcatcttctt cgataatact ttctaaaagt tttactttag aaatgtctac
    42601 taaatttccg cctttagcag gttcagcttt tcctacagaa gataatattt catcactttt
    42661 tggggcgaaa gttttaatag gggcagtgta ttttttcttt tcttcatcag taaaatcttc
    42721 ttttaagagt ataagggtat ttgttagtgc tctaggtgtt ccaatttcat aatcccacca
    42781 atttaagttt gtattctttt gaggtgctga ttttgagaaa tttgttttta gttcttcaag
    42841 ttttttcacc ggttcttttc cataggcatt tttatgcagc cactcaaggg catctttaac
    42901 tttgttttta ttatccggag tatttaactt agtatcttta tgcttcatcg cttctgcgat
    42961 tttctcaata ttacgatagg tacgagtcat atccgcagac ttgttatcta aatcttttgc
    43021 actatcccac aagtaagtcc ttccactttc agttttcatg ctactgagta atttctctgc
    43081 ctctttctcc gtagcatcaa acttcttctt cattgcgtca ttcttctcat catactgatc
    43141 ataaccgtag ttaacgttca gccatgtgtt cctcaacttt tcataatctg gcgtttgaac
    43201 attcgtatca gccacagcga tttgattttt atcaatactt ctgaattcac caccattcaa
    43261 agtaatcgca ccagccatta ataacgtaat ggtggataat ttttgccatt tcttcattct
    43321 atatgtcatt gacatgtctc cttttgtgtt gcgcgtgcgc aatgaatatt atgattaaat
    43381 aatgattcaa tttttcaaaa ttcgttaacg tatacaaatg actgtctact gtcaaacaat
    43441 ccacaaagaa tgttgatgca tataaacaat cgatcaccca aattttccgc atcacccaaa
    43501 aattgcaacg aaaataattt ttaaaaaatt aaatattatc aatcttagtg attatatcat
    43561 tttaaatatt taagttatac aatttttaaa gtaattaaaa acgatgaaat gtaaaatgtt
    43621 aaaaaagaga caaatttaaa tttgagtaat aaaataagag gtattcaaac gaggatggta
    43681 atatataaag caacattact taagatgcca ccaacataac tgaaaggata gtcgcaatga
    43741 aaactataga agtcgtagcg ctcatgaata tatctcaaga cactttaaga tattatgaaa
    43801 aggttggtgt gattccacct gttaatcgag atgaaaatgg atatagaata tataatgata
    43861 gtgatttaaa ttggatttat ttagtgaaaa atttgcgaaa tgcaggcgtc agtattgaat
    43921 cgcttatcga attttgcagg ttagcgcagc ttcctaaaaa tgaaaatatt caagcacagc
    43981 aaaagcaaat tttaaataag caactcgaag aattaaatga aaacttaaag acaattcatg
    44041 atgcgagaga tttactacaa tataaaattg ataattatga taatcatatt gctaaaatca
    44101 atgctagtga taattatgat gacaatgttg aacgtctttg ggagagaaag taaacctatg
    44161 gcttgctata gagttcgctc catggtttat agtggactta caagataaag gaggttcatt
    44221 ttatgaatca tattgaaata agtaaagatg taaagatacc agtattaggc ttcggagtat
    44281 ttcaaattcc tcaagaacaa acagcagaag cggttaaaga agcaattaaa gcgggctata
    44341 gacatattga tacagcgcaa agttatttaa atgaaactga agtaggtcaa ggtattgaag
    44401 cttcaggtat tgatagaagt gaactattca ttacgactaa agtatggatt gaaaatgtaa
    44461 actatgaaga tactatcaaa tcaatcgaac gatcattaca acgattaaat ttagactatc
    44521 ttgatctagt gcttattcac caaccttata atgatgtata tggctcatgg cgtgcattag
    44581 aagagttaaa agaaaatggt aaaattaaag caatcggtgt atctaacttc ggcgttgatc
    44641 gaattgtaga tttaggtatt cacaatcaaa ttcaaccaca agtgaaccaa atagaaatta
    44701 atccattcca tcaacaagaa gaacaagttg cagcacttca acaagaaaat gttgtggtag
    44761 aagcttgggc accatttgca gaaggtaaaa atcaattatt ccaaaatcaa ttgttgcagg
    44821 ccattgcaga taaatataac aaatcaattg cacaagttat tttacgctgg ttagttgaac
    44881 gtgacattgt tgtactcgca aaatcagtaa atccagaacg tatggctcaa aacttagata
    44941 tatttgattt tgaacttaca gaagaagata aacaacaaat tgcgacatta gaagaaagta
    45001 atagccaatt tttctcacat gctgatccag agatgattaa agcacttaca agcagagaat
    45061 tagatgttta atcataatat aacaaagtaa ataattttca ttacgctaac ctgaaacttg
    45121 gatgttaaag tctatgtttc aggttatttt attactctta aaaagtgaaa ggagcaagca
    45181 aataagttat tatatatttt tatattatgt aataacttaa ctaaggttat aattagataa
    45241 ttaatatgtc ttggaattaa ttatctaata agttgtatag caggtagcga tatgacatat
    45301 aaacactttt ctattattac attaattaca ggtataatta tgcatatgac aatgtacttt
    45361 gtaccatttg aatgtcggaa aatgccatta tttatgatga ttatttttac aatctaaaag
    45421 cagtaaagac tcataatata gccaaaaacg ttaataaaag tttattaaat gacaagggtg
    45481 tgagtattgg taaatttact caaaaggtta aagggaaaaa tcctacctgg agagatccaa
    45541 aaactaaatg gactatttcc aaaaataaag ggcaatcaca tggtggttct tattggaagt
    45601 taatcaataa caaaggaaaa agaatagctt ctttaactaa agaaggaaaa atcttaaggg
    45661 aataaggtgt tgtaaattat gtactttgat ataaataatt tggatataaa aaagttgaat
    45721 tttagaaagg taaaaaacgc aattcattta agtttaagtg gcgataaata ccagtttttg
    45781 gaggacggta aaactatcaa taatacttat tttttagctg tatatgataa tgcaataaac
    45841 atttttgaag atttgttttc gccttctgat tctataaatt tggttcatat agtatatgtt
    45901 tataatgaac cttataagaa aacaaatgtt ataaaaaaat ttacatcgtt aacaatagat
    45961 gaattgtatt actttaaaga atataaaaag gagaaggatg ataatatttt gtgcgaggag
    46021 ttttcatata agtgttccat aaaagatata aaatataaaa aattattaaa agctatatcc
    46081 aatcaagact ttaaagggtt agttccaaat atcaatcaga atgataatta ttctgaaata
    46141 tatataatta acaatactaa aaacttaata tatcatttat atgatgatag aggtctatgg
    46201 ctagctttta ataataatga agattatgca agatattctg aaaaatataa tgatttaata
    46261 tcggaatttg ataatgaaga tatctaattt tttaatattc gtagctttta tttttctact
    46321 tattttatgt ttatttttaa tcttacaaat gacaaaccat taaaagtagc atcccaacat
    46381 caaacaaaaa acaatccatc aaataaaaat cgctataaaa ccaagcaagt cattctatac
    46441 tcaataaata aaatttctca ctcattataa tttggaattc caaatgtcga attccgaaaa
    46501 ccaaactcca aattccaaaa acgcaactcc aaaattaaaa gcatttcccg agtaacaggg
    46561 aaatgctttt tacgtactga ttactctgtc attaatgatt ttacaacgga aaccatgtcg
    46621 tcatgtatga ccaaagtagc gtcgctatca taaggtgttc gatctttatt gataataatt
    46681 aaattgtcgc ctttaaaatt tgatattaat cctgcggcag gttgtacaac gagtgatgaa
    46741 cctagtacaa caagggtgtc agcatgttca attttattta atgcccttat gatggtaggt
    46801 tgatctaata tttcaccgta taatacgatg tccggtcgaa tggcaccacc acaattatca
    46861 caatgtttca aagttctatc aatgacattt gatttcgtat aagatttatg acatgcatta
    46921 caataaaaac gatttaacgt gccatgtaat tcatcaacat gttgacttcc agcgtctgag
    46981 tgcaaaccat cgatattttg cgtgatgaca cctaaagatt gttgattacg ttctaatttt
    47041 gcaatccaat catgaacgat attaggcttc gtatcgacaa atagtaaacg cttatggcag
    47101 aaattgataa aaccttcagg atcatcttct aaataatcac ggcttaacaa gtattctggc
    47161 gaaagtccat cttttgaaat ttcatcaaat aagccaccca ttgaacggaa atctggaacg
    47221 ccacttgcga cagatacacc agcacctgtg aaaaatgtaa tacgattcga actatctata
    47281 atatgtttta atgtctctaa atcatttctc aaaataaaca ccccatcaaa ataatgaatg
    47341 atttataacg tcttttgctt ttattatagt atattcat
//
LOCUS       DAHHYJ010000004        43667 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000004 DAHHYJ010000000
VERSION     DAHHYJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 43667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43667
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.004"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..307
                     /gene="lpl5"
                     /locus_tag="NBK22_000168"
     CDS             <1..307
                     /gene="lpl5"
                     /locus_tag="NBK22_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein Lpl5"
                     /protein_id="HCD2413389.1"
                     /translation="KFFGQYANFKDLENYNNGDVSINWNVPSYDVEYKMSNKDENVKQ
                     LRSRYNIPTDKAPMLKMHIDGDLKGSSVGYKRLEIDFSKEDRDISVIDYLSYKPAKK"
     gene            339..1130
                     /locus_tag="NBK22_000169"
     CDS             339..1130
                     /locus_tag="NBK22_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2413390.1"
                     /translation="MEYLKRLALFISVIILTIFIMGCDSQSDTAENPKEGSKEAQIKK
                     SFSKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWTISTDFAKSNKPGEMDSEGMVL
                     HLNRNTRTATGHYTIRTTYDELGKMTREKNYRVELKNNKIVVLDKVEDVNLKHKIENF
                     KFFSQYADFKDLKNYKNGRISINENVPYYEAEYKRNNSDGNVKKLREKYPITTKQSPI
                     LKLHIDGDIKGSSVGYKQIEYMFSKEKEDETFMSDFLNFGPSHSN"
     gene            1182..1979
                     /locus_tag="NBK22_000170"
     CDS             1182..1979
                     /locus_tag="NBK22_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2413391.1"
                     /translation="MGYFKRVLLYIIVMVLSVFIIGCDKSSDTSEKSKGDSKEAQIKK
                     SFAKTLDMYPTENLEDFYDKEGYRDGKFKKGDKGTWVIRSEMTTELKNENMVSKGMVI
                     RLNRNSRTCTGEYFVRIVKEDSEGKVYSDERKYPVKMENNKIIPLKPIDDEKVKKEIE
                     EFKFFVQYGNFKELENYKDGEVTYNPEAPIYSAQYQLKNSDYNVEQLRKRYNIPTQKA
                     PKLLLKGSGNLKGSSVGYKNIEFTFVENKEENIYFTDSVYFNPSEDK"
     gene            2161..2973
                     /locus_tag="NBK22_000171"
     CDS             2161..2973
                     /locus_tag="NBK22_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2413392.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKDEQKKEEQTNKTDSKEE
                     QIKKSFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKADELSNE
                     GMVLYLDRNTRTAKGHYFVKTFYEKDKFPDRKKYKVEMKNNKIILLDKVEDPKLKKRI
                     ENFKFFGQYANLKELKNYNNGDVSINENVPSYDAKFKMSNKDENVKQLRSRYNIPTDK
                     APVLKMHIDGNLKGSSVGYKKLEIDFSKGEKSDLSVIDSLNFQPAKVDEDER"
     gene            2963..4288
                     /locus_tag="NBK22_000172"
     CDS             2963..4288
                     /locus_tag="NBK22_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413393.1"
                     /translation="MKDKYKIDPGPIKYNTEETTAISKISYEIENAKLYGIKSKTIGR
                     QIDQLKEAKKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKKL
                     KEGEFSRQNFTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFIT
                     GHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITRF
                     VAENDALTENLKKYKHYVFFGNDKVFKNGKGHGMEGFLTKEEQKAIKKELKKLQGYAE
                     ENNKSFVKHSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQL
                     IDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMVR
                     EPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            4285..4608
                     /locus_tag="NBK22_000173"
     CDS             4285..4608
                     /locus_tag="NBK22_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413394.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKQL
                     DKLSD"
     gene            4627..4941
                     /locus_tag="NBK22_000174"
                     /pseudo
     CDS             4627..4941
                     /locus_tag="NBK22_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5264..5458)
                     /locus_tag="NBK22_000175"
     CDS             complement(5264..5458)
                     /locus_tag="NBK22_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413395.1"
                     /translation="MLFFILALYVIGIAFILLSVFGSKTEGLSTKHTLYTIGSAIITI
                     AIFISIGYAIQYLTAALYGL"
     gene            5645..6847
                     /locus_tag="NBK22_000176"
     CDS             5645..6847
                     /locus_tag="NBK22_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD2413396.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLETMPNNVVRSKGIVWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(7491..7553)
                     /locus_tag="NBK22_000177"
     CDS             complement(7491..7553)
                     /locus_tag="NBK22_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCD2413397.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(7617..7685)
                     /locus_tag="NBK22_000178"
     CDS             complement(7617..7685)
                     /locus_tag="NBK22_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCD2413398.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(7737..7802)
                     /locus_tag="NBK22_000179"
     CDS             complement(7737..7802)
                     /locus_tag="NBK22_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCD2413399.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(7834..7899)
                     /locus_tag="NBK22_000180"
     CDS             complement(7834..7899)
                     /locus_tag="NBK22_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCD2413400.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            8420..9904
                     /locus_tag="NBK22_000181"
     CDS             8420..9904
                     /locus_tag="NBK22_000181"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCD2413401.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMPMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHMSNSTWSLLTNVLLVLVVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILGWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYIITHNYFYDVLQNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKAFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            9917..12622
                     /locus_tag="NBK22_000182"
     CDS             9917..12622
                     /locus_tag="NBK22_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCD2413402.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHYYNIPQHYINLYID
                     NIKTLKDVPASYMDHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPKMTIESVLTHFSIDQEDYQAYVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESLAGALDAIQVNVSENDNANQS
                     HSVSLNATQAVDENNSELNQVGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNTIMPRKSRVSLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQSGVYIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSSIALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGKLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVVQAPDYVVARLLANNDHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            12784..13146
                     /locus_tag="NBK22_000183"
     CDS             12784..13146
                     /locus_tag="NBK22_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCD2413403.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIILKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(13375..13791)
                     /locus_tag="NBK22_000184"
     CDS             complement(13375..13791)
                     /locus_tag="NBK22_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413404.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYIYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            13829..14503
                     /locus_tag="NBK22_000185"
     CDS             13829..14503
                     /locus_tag="NBK22_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD2413405.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIVTIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            14540..15274
                     /locus_tag="NBK22_000186"
     CDS             14540..15274
                     /locus_tag="NBK22_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCD2413406.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNDQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSDILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            15452..15565
                     /locus_tag="NBK22_000187"
     CDS             15452..15565
                     /locus_tag="NBK22_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413407.1"
                     /translation="MGIAPEVVMWRDVGDIDGVVCGEMLAHVYSVEPEILM"
     gene            15730..17067
                     /locus_tag="NBK22_000188"
     CDS             15730..17067
                     /locus_tag="NBK22_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCD2413408.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            17284..18189
                     /locus_tag="NBK22_000189"
     CDS             17284..18189
                     /locus_tag="NBK22_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCD2413409.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLVIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDTEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLTEGTI
                     VVVFPDGSDRYMSKQIFEYEENNNE"
     gene            18182..19324
                     /locus_tag="NBK22_000190"
     CDS             18182..19324
                     /locus_tag="NBK22_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCD2413410.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIATLENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKNTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            19622..20647
                     /locus_tag="NBK22_000191"
     CDS             19622..20647
                     /locus_tag="NBK22_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCD2413411.1"
                     /translation="MIEFRQVSKTFNKKKQTIDALKDVSFTVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASNGQVIVDGHDITNYSDKMMRDIKKDIGIIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNIDQQFDDTAINQYFKEKN
                     IQFEEVH"
     gene            20651..21310
                     /locus_tag="NBK22_000192"
     CDS             20651..21310
                     /locus_tag="NBK22_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2413412.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPIINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            21347..22189
                     /gene="gmpC"
                     /locus_tag="NBK22_000193"
     CDS             21347..22189
                     /gene="gmpC"
                     /locus_tag="NBK22_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCD2413413.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            22521..23525
                     /gene="aaa"
                     /locus_tag="NBK22_000194"
     CDS             22521..23525
                     /gene="aaa"
                     /locus_tag="NBK22_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCD2413414.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRG
                     YNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPT
                     VGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH
                     "
     gene            complement(23710..23979)
                     /locus_tag="NBK22_000195"
     CDS             complement(23710..23979)
                     /locus_tag="NBK22_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413415.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            24128..24523
                     /locus_tag="NBK22_000196"
     CDS             24128..24523
                     /locus_tag="NBK22_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD2413416.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVHALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWIDKDNDALIAPAVKVWIETYGGKHDK"
     gene            24513..24998
                     /locus_tag="NBK22_000197"
     CDS             24513..24998
                     /locus_tag="NBK22_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2413417.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SNNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAAFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQDINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(25167..25949)
                     /locus_tag="NBK22_000198"
     CDS             complement(25167..25949)
                     /locus_tag="NBK22_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD2413418.1"
                     /translation="MNAVFILAVLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(25946..27064)
                     /locus_tag="NBK22_000199"
     CDS             complement(25946..27064)
                     /locus_tag="NBK22_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD2413419.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTILTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRNIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(27179..28063)
                     /locus_tag="NBK22_000200"
     CDS             complement(27179..28063)
                     /locus_tag="NBK22_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2413420.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPSLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            28245..32744
                     /gene="gltB"
                     /locus_tag="NBK22_000201"
     CDS             28245..32744
                     /gene="gltB"
                     /locus_tag="NBK22_000201"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCD2413421.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILG
                     SEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKR
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKIYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYKTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            32762..34225
                     /locus_tag="NBK22_000202"
     CDS             32762..34225
                     /locus_tag="NBK22_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCD2413422.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKNKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFEE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            34494..34586
                     /locus_tag="NBK22_000203"
     tRNA            34494..34586
                     /locus_tag="NBK22_000203"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34530..34532,aa:Ser,seq:tga)
     gene            35057..36484
                     /gene="treP"
                     /locus_tag="NBK22_000204"
     CDS             35057..36484
                     /gene="treP"
                     /locus_tag="NBK22_000204"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCD2413423.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKQAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGPVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            36548..38188
                     /gene="treC"
                     /locus_tag="NBK22_000205"
     CDS             36548..38188
                     /gene="treC"
                     /locus_tag="NBK22_000205"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCD2413424.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKKILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLTILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLACEGRVVIQTGTVENNTISGFGAIVIETNA"
     gene            38213..38941
                     /gene="treR"
                     /locus_tag="NBK22_000206"
     CDS             38213..38941
                     /gene="treR"
                     /locus_tag="NBK22_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCD2413425.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVVYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRTK"
     gene            39003..39272
                     /gene="ffs"
                     /locus_tag="NBK22_000207"
     ncRNA           39003..39272
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NBK22_000207"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            39341..39442
                     /locus_tag="NBK22_000208"
     CDS             39341..39442
                     /locus_tag="NBK22_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413426.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDAQFE"
     gene            39580..40104
                     /locus_tag="NBK22_000209"
     CDS             39580..40104
                     /locus_tag="NBK22_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD2413427.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            40173..41870
                     /gene="dnaX"
                     /locus_tag="NBK22_000210"
     CDS             40173..41870
                     /gene="dnaX"
                     /locus_tag="NBK22_000210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCD2413428.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDEALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPTQKSSKKPVRGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEK"
     gene            41960..42277
                     /locus_tag="NBK22_000211"
     CDS             41960..42277
                     /locus_tag="NBK22_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCD2413429.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            42284..42880
                     /gene="recR"
                     /locus_tag="NBK22_000212"
     CDS             42284..42880
                     /gene="recR"
                     /locus_tag="NBK22_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCD2413430.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLTFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKNDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            43083..43181
                     /locus_tag="NBK22_000213"
     CDS             43083..43181
                     /locus_tag="NBK22_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413431.1"
                     /translation="MNWIIELDLRLWCVGAINLINAVNDYANEILL"
BASE COUNT        14944 a         6212 c         8912 g        13599 t
ORIGIN      
        1 taaatttttc ggacaatatg caaattttaa ggatttggaa aattacaaca atggcgacgt
       61 gtcaataaat tggaatgttc caagttatga cgtggaatat aaaatgagca ataaagatga
      121 aaatgttaaa caattaagaa gtcgttataa cattcctact gataaagctc caatgttaaa
      181 aatgcatatt gacggggact taaaaggtag ttctgttgga tataaaaggt tagaaataga
      241 tttttcaaaa gaagataggg atatttcagt cattgattat ttaagttata agccagcgaa
      301 aaaatagtac tgataattaa atattagggt gtgaaggaat ggaatatcta aaaaggcttg
      361 cattgttcat aagtgttatt attttgacca tttttataat gggttgtgat agtcaaagcg
      421 atactgcaga aaatccaaaa gaaggttcaa aagaagcaca aattaaaaag agtttttcga
      481 aaacgttaga tatgtatcca attaagaatc tcgaggattt atatgacaaa gaaggatatc
      541 gtgatggcga atttaaaaaa ggtgataaag ggacgtggac aatatcaaca gattttgcta
      601 aaagcaacaa accaggtgaa atggatagtg aaggtatggt gctacatttg aatagaaata
      661 cgaggacggc aactgggcat tatactataa gaacaactta tgatgaattg ggtaagatga
      721 cacgcgaaaa aaattatcgt gttgaactta aaaataataa gatagtagtt ttagataaag
      781 tagaagatgt aaaccttaaa cataaaattg aaaattttaa attttttagt cagtatgcag
      841 attttaaaga tttgaaaaat tataaaaatg gaagaatatc tatcaatgaa aatgttccgt
      901 attatgaagc agagtacaaa aggaataata gtgatggaaa tgtaaaaaaa cttagagaaa
      961 agtacccaat tacaaccaag cagtctccaa tattaaaact gcatatagac ggagatatta
     1021 aaggtagctc agttggatat aagcagatag agtacatgtt ttctaaggag aaagaggatg
     1081 agacttttat gagtgatttt ttgaacttcg gaccatcaca tagtaattag taaataaaac
     1141 caggggcata aactaattta aaacaagaga gaagtgaaaa gatgggatat tttaaaagag
     1201 ttttacttta tataattgtt atggttttga gtgtttttat aataggttgt gataaatcaa
     1261 gcgatacttc agaaaaatca aaaggagatt caaaagaagc acaaattaaa aagagttttg
     1321 caaaaacgtt agacatgtat cctactgaaa atctagaaga cttttatgac aaagagggat
     1381 atcgagatgg aaaatttaaa aaaggcgaca aaggtacttg ggttattaga tctgaaatga
     1441 caacagaact gaaaaatgaa aatatggtat ctaaaggtat ggtcatacgt ttaaatagaa
     1501 atagtagaac atgcactggt gaatattttg tcaggatagt taaagaagac agtgagggca
     1561 aggtatatag tgatgaacga aaatatccag tgaaaatgga aaataataaa atcattccat
     1621 taaaaccaat cgatgatgaa aaagtaaaaa aagaaattga agaatttaaa ttctttgtac
     1681 aatacgggaa tttcaaagaa ttggaaaact ataaagacgg agaagtgaca tataacccag
     1741 aagcaccaat atactctgca caatatcaat tgaaaaacag tgattacaat gtagaacaat
     1801 tacgtaagcg atataatatc ccgacgcaaa aagcgcctaa attattattg aaaggctcag
     1861 gtaatttaaa aggttcatca gtcggatata aaaatattga atttaccttt gttgaaaaca
     1921 aggaagaaaa tatttacttc acagatagtg tctactttaa tccaagcgag gataaataag
     1981 tataattatt attataaaaa atgacgttag aacatttaat aataaagtta aatgaattac
     2041 atgtattaaa ttgaataagt tagttttgtt tgataacata aaagttgaat aatcactatt
     2101 ttaataagtt atatgaaata ttcaaatgtt tataaaatta aagcagagag atgtgaaatg
     2161 atggagtata taaaaaaaat tgctttgtac atgagtgtat tacttttaat catttttatt
     2221 gggggatgtg gaaatatgaa agatgaacag aaaaaagagg aacaaacgaa taaaacagat
     2281 tcaaaagaag aacaaatcaa aaagagtttt gcgaaaacgt tagatatgta tccaattaag
     2341 aatctcgagg atctatatga caaagaagga tatcgagatg gtgagtttaa aaaaggtgat
     2401 aaaggtatgt ggacgatata tacagatttt gccaaaagta ataaagcaga cgaattaagt
     2461 aatgaaggta tggtcttata cttagataga aatacacgga cagcaaaggg acattatttt
     2521 gttaagacat tttatgaaaa ggataaattc ccagatagaa aaaaatataa agttgaaatg
     2581 aaaaataata aaattatctt attagacaag gtagaagatc caaaactaaa aaagagaata
     2641 gaaaacttta aatttttcgg acaatatgca aaccttaaag aattgaaaaa ttacaacaat
     2701 ggtgatgtct caattaatga gaatgttcca agttatgacg caaaatttaa aatgagcaat
     2761 aaagatgaaa atgttaagca attaagaagt cgttataata ttcctactga taaagcaccg
     2821 gtattaaaaa tgcatattga tggtaatttg aaaggaagtt ctgtgggtta taaaaagttg
     2881 gaaattgact tttcaaaagg tgaaaaaagc gatttgtcag taatagattc attaaatttc
     2941 cagccggcga aggtggatga agatgaaaga taaatacaaa atagatccag gacctattaa
     3001 atataatact gaggaaacta cagctatttc taaaataagt tatgaaattg aaaatgcgaa
     3061 attatatgga attaaaagta aaacaatagg tagacaaata gatcaattaa aagaagcgaa
     3121 aaaattccct tctaatcttg aatatgttga tagttatact gattctctta ctggagtaac
     3181 aacttctgct tttttaaata aagatacagg caaagtaact ctcgggatga ctgggactaa
     3241 tttacaagac gaagccttta aaaagttaaa agaaggtgaa ttttcaagac aaaattttac
     3301 caatgctttg gaaacagtta aagatggata tgcagatctt aaaatattat attctcctgc
     3361 atctgatcaa aactatagat atgcgaatac acaagaattt ataaataaaa taaaaagtaa
     3421 gtatgacatt gattttatta ctggtcattc actaggtgga agagatgcag tagttctagg
     3481 aatgagtaat ggtattccga acattgtggt ttataatcca gctcctattt ctataactag
     3541 tttgaatcct aattccccag atggaaaacg attgttagag ttatataaaa attataaagg
     3601 taatattact aggtttgttg cagaaaatga tgcattgaca gaaaatctga agaaatataa
     3661 gcattatgtt tttttcggta atgataaagt ctttaaaaat ggtaaaggtc atggaatgga
     3721 aggctttctg accaaagaag aacaaaaagc tataaaaaaa gaactaaaaa aactacaagg
     3781 ttatgcagaa gaaaataata agtcatttgt aaagcattca aataatgcta tctctaaatt
     3841 agctagtata gaattactta gagcaaatat gatgactaca aatggaggag gattatcttc
     3901 ttcgcagcaa aaagttttag aaagtttaac agctttaaca attgcgcagt cattcagtca
     3961 actgatagac gatgaaatta atcaaatcaa aaaaatgtat aatgaaaaga aaaagaaatt
     4021 tggaaaaaat tgggaagacg ctcaaaaagc tggaaaagct gtaggtgaag atttgagtgt
     4081 aaatggagtt ctcaatgctt tagatgaagg tcaagtgaat gaaagtagta tggtaagaga
     4141 acctgaacaa atgatatctg caaaagaaag acaactttca acgatagggt cttctgtatc
     4201 aaattatatt atgagagtta gactcagtat taatgaaatc gttgataaag atcaagtgct
     4261 tgcatcacaa ataggtggat tactatgatg tttaatcaaa ttaataataa aaatgaatta
     4321 gaagaatcat atgaatctga gaaaaaacgt atagagaatg aactgcaaaa tttaaatgaa
     4381 cttaggcata gaactcgaaa agaaaatgaa cgtagttatg atgtttttca atatttgaag
     4441 cacgaaatga attatagtga agatgcccaa aggaaaatga cgagaaatat agaagcgtat
     4501 gagcaagaaa tcaatgagat aattagaaag caagaatgga aattagaaga atataaagaa
     4561 gacttaaaaa agtcttatga aaagcagtta gataaactaa gtgactgata atttggagga
     4621 gattaaatga ctctaataga accagatatg aacttaagaa tgccagatat aagtactaca
     4681 gtagaaacac ttaatctcat atctaaaatg gaagcgtaaa aagaaaatat tcgcacagtt
     4741 attgcacctg aacataagca taaatacaaa gatattgaaa acggattaaa aggtgaagaa
     4801 aaagtattaa ttgaacaaat ggcgcagcat tgcgaagctt ttaaagctaa ttttaaaggc
     4861 gcagctcaag gagattgggt taaaagtgct atgtctgaga ttgacagcat taaggatgac
     4921 ctgaaaaaaa ttaatagcta aactacatat catattaata aataaagctc ccttcaaaat
     4981 tttcattttt tcaatgtcta ctttggaggg agcgcttcag tatattcaca actgacacta
     5041 ggccgcttct ttttaattta ttcccatgta aagtaattcg aaccgaatgg aaatatcaca
     5101 ctcacaattg taaaaacaca acttaaatta ctgtcccgaa ttaaacataa ccacattcgt
     5161 aatagcaaca acaaaatcct caagtaccaa aagctatgac ctctttatca aaatcaacgc
     5221 aagttaacct ctcccgaact acccgccaca aatcatcttc accttacaaa ccataaagcg
     5281 ctgcagttaa gtattgaatg gcatagccaa ttgaaatgaa aatagcaatc gttataatag
     5341 cactgccaat ggtatataaa gtatgtttcg tagataatcc ttcagtcttt gaaccaaaaa
     5401 cactgagtag aataaatgca atgccaatga catataaggc taaaatgaaa aataacatgt
     5461 ttatacacct cgattctctc tcgtgatata tatatctttg tctccattat aaagtaaagt
     5521 gctcaataat ttaattaagt atactatttt tttattatcg aagattgcga aagaggaaac
     5581 gctcctatat aatgtaaaac gtaattattc ctatttacaa ataaattgaa gtgagggtat
     5641 atatatggct aaaattccag ttacggtatt aagtggttat ttaggctcgg ggaagacaac
     5701 gttgttaaat catattttac aaaatcgaga aggtcgacgt atcgcggtaa ttgtaaatga
     5761 tatgagtgaa gtcaatatcg ataaagatct tgtcgcagat ggtgggggac tatcgcgtac
     5821 agatgaaaaa ttagtcgaac tctctaatgg ttgtatctgt tgtacactta gagatgattt
     5881 attaaaagaa gttgagcgtt tagtgaaaaa aggtggcatc gatcaaattg ttattgagtc
     5941 aacagggatt tcagagccag tacctgttgc acaaactttc tcatatattg atgatgaact
     6001 tggcattgat cttacagcga tttgccgttt agatacaatg gttacagttg ttgatgctaa
     6061 ccgcttcgta catgacatca actcagaaga tttattgatg gatcgtgatc aaagcgttga
     6121 tgaaacagat gaacgttcga ttgctgattt attaattgac caagttgaat tttgtgatgt
     6181 attgattatt aataaaattg atttaattag tgaagaagaa cttgcaaagt tagaaaaagt
     6241 gttaagcgca ttgcaaccga ctgctaaaat cattaagaca acaaattctg aagtagattt
     6301 aaaagaagtc ttaaatacgc agcgttttga ttttgaaaaa gcgagcgagt cagcaggatg
     6361 gatcaaagaa cttgagtctg gtgggcatgc atcgcataca cctgaaacag aagaatatgg
     6421 tatatcatcg tttgtatata aacgtcgact tcctttccat gctaaaaggt tcaatgattg
     6481 gttagaaacc atgccaaata atgtcgttcg atcaaaaggt atcgtatggc tagcacaata
     6541 caatcatgta gcatgtttat tatctcaagc agggtcatct tgcaatattc acccagttac
     6601 atattgggtg gctagtatgt ctgaagcgca acaaacacaa atattagcag aacgtcaaga
     6661 tgtcgcagct gaatgggatc cagaatatgg cgatcgtcat acacaatttg tcattattgg
     6721 tacagaatta gatgaagaaa aattaacaaa agaactcgat gcatgcttag tcaatgcgca
     6781 agaaattgat gcagattggc aacaatttga agatccatat caatggcaaa ttagaccagc
     6841 acgataagtt gaatgaagta taatcatttt taaattgtgg cttaattgtt tgcaatttat
     6901 gacaattaat aaaaagttaa gatgttctta ggatcttttt gttttcaatc tatctaaagt
     6961 gtgatagttt tgataaagca gaaatttgca ttttatccat agggctagga catgtatgtg
     7021 tcttagtcct ttttatattt acgttgatga aaaatgataa tcatcaaatg aaagcacaat
     7081 cattgtttaa cggtcataaa aatgagtgga gtaaatgtag ctatcaatgt actattaccg
     7141 cttacggtca cacgagtgtt tcagtctaaa cgtgcaacgc aattccgtac gtggtcaata
     7201 tatcttgcgc tagatcatga aaatcattaa gatttcgcac gccaaatcat gaatgtctca
     7261 gacctcgagt atcaaacata aaacacgcca caaaaatata acgcgtcaga ctccaaagaa
     7321 cacgaatatt ccgtacgtcc caccaagaac atcctataca aaaaaaccaa gaccatttat
     7381 ttgcaacaaa ctaacaagac taacctcaca tcaataaatc aacacaaagc aaagccacca
     7441 tccctattgg catagtggcc taagaatttt aagtattcaa ttcgcttaaa ttattttgcg
     7501 aaaatgtcga taattgcttt gatgatttta atgatagtac ctacaatagc catcgttttg
     7561 tcctcctgta tgttgattta acatagtgat gagttgttga tcgttaatgt ttgagattag
     7621 ttgttaccta aaaatttacc aagtaaatct ttaaagaatt tgaataattt tgctacgaat
     7681 tccatgtgaa tggccccctt caaataagat gttcatatct atgagatttt tataacttac
     7741 ttaccagtga atttctcaat taatccttta atgaatttaa tgattcctgc aatgataccc
     7801 atgtgaaaga cctcctttgt ttgttatgaa atcttattta ccagtgaatt gttcgattaa
     7861 gcttttgata actttaatga tgccagcgat gatacccatt aagattacct cctttgctta
     7921 tgagttaact tcattgtaca tagttatctt gtgcgtaatt gatttttttc ctaacttgaa
     7981 ttgttatgat cataactgcg aattttttat tttaaaagga cgggaaatac tgcctaactg
     8041 tacaggtgca tttacaaaat ctttacaatc attggtttaa tagaggttaa gctcatgaat
     8101 ttgacatgaa gcagaaattt aattatattt tatgttataa gtttaaaacg aataacacgt
     8161 taggtctcgt ctaggcaaag catatttatg aagatgaatt gaacagacag aagtaacatt
     8221 ttatatacat atgtgcatgg gagcacgctt aaagagaaca ctgcaacaag gtatatcaac
     8281 gtaccgtgat gcagtgtcta gttaaactga aacctgcaat agtaaatttt ctgccattat
     8341 gtacagaatc tactattgta ggtttttatt tttatacata agcaattatg cgagagatta
     8401 gaaataagga ggttatgcag tgttaagttt tcaattgcta ttttcactgt ttgttattgc
     8461 gcttatcatt gcattgataa gtggcttgtt gtttttagca ccagttatgc caatgagata
     8521 tattaaatta catttataca tactagtcat gccagtatta tttgcagtca ttggcttttt
     8581 cggtattcat ggtcaacatg tcttaggtcc atttaaaata gatcgtttat cttggttatt
     8641 agctggcttt gtaatggcgc ttggttttat tattcaaaag ttttcaatgc gatatttact
     8701 aggtgatcat cattatagac attactttcc attgttcact gcgattacgt cgtttgcatc
     8761 tttagcatgg atgtctgaag acttaagact gatggcactc tgctggggta tgacattatt
     8821 atgtttaaca ttgctgatga acgttaatcg tttttggaaa gtgccacgtg agtctgcgaa
     8881 attatcaagt atgacatttt tatgtggttg gcttgcattc gttggagcaa ttgtaactat
     8941 ttatattgcg actggcgagt ggcgcgtgcc tcaacatatg tccaattcga catggtcatt
     9001 gttgacgaat gtactacttg tattagttgt catgatacca gcagcacaat ttccatttca
     9061 tcgatggttg attgaatctg taacggcacc aacgccagta tcagcaatta tgcatgcagg
     9121 aattgtgaat gcaggtggtg ttattttaac tcgttttgcg ccgatatttg ataatggatt
     9181 tgcgttatca ttattactta tcctttctag tatttctgta ttgttaggat cgggtattag
     9241 cttagttcaa gttgactata aacgccaatt agtgggctct acgatgagtc aaatgggctt
     9301 tatgttagtt caatgtgcat tgggtgtata ttcagcagcg attattcatt taatattgca
     9361 cggtattttt aaagcaacat tatttttaca atcaggttct atcgtgaagc gattcaatat
     9421 tccaaaacaa gcatctgcta aagacgctta tggctggatt gtcatgggac gtgtattagc
     9481 tattatcgtg gcatttctat tttggatgag tagtgacaga agtgcatatg aagtgttaag
     9541 tgcactcatt ctaggatggt cattacttgt atcttggaat caaatggtag cctttagtaa
     9601 aggacgcatg gcacgtcttg ttggtatgat tttgattgca atcgtgacat ttatctacat
     9661 catcacacat aattattttt acgatgtatt acaaaatata acaacacatg cgacaacgcc
     9721 gcctacagtg agtgtcatca ttagtgttgt cattttaatc tttggcagtt tattaagtat
     9781 ttgggtggcg cggcatcgat actctaaggc ttttgcggta ttgtacgtgt ggttagttaa
     9841 tctaggtgaa gcacgctcga aagcgataga aagtcatccg aattatttga agaagtattt
     9901 ataggaggtg aaaggtatga caacacagtt aaatatcaat tcagtcattg aaaatgcgaa
     9961 acgtgttatt acaccattat caccaatttc gatttttgca gcacgaaatc catgggaagg
    10021 attagaagcg gatacgtttg aagatgtcgc aaaatggtta cgtgacgttc gagatgtgga
    10081 tattttccca aataaagcat taatagaaag cgctgtggca cgtggtgaat tagatgaaag
    10141 tgtctttaat caacttgtta ctgatatgtt acttgaacat tactacaata tcccgcaaca
    10201 ctacatcaat ctttatattg ataacattaa aacattaaaa gacgtacctg catcatatat
    10261 ggatcattca aatgttgatg ttgttgctga tctactatta gaaaaatcaa aacgtgatat
    10321 ggctgaatca tatcatcact atgatgtacg tccgatgagt gatgcaataa tagatgaaca
    10381 aggtgagcca cttagcgaac aagtgaatcg tcaaatgatt aaatggacga aactttatat
    10441 cgatcaattt ctatcgagtt ggacaatgcc gaagcgtgag caaagttttt accatgcatg
    10501 gttgcattta gcgcaacatg atcatagttt tactaaagca cagcgccaag tgattaaagg
    10561 cttgcccaat gatcctaaaa tgacgataga gtcagtatta actcattttt caatagatca
    10621 ggaagactac caagcttatg ttgaaggaca tcttttggcg ttaccgggtt gggcaggtat
    10681 gttgtattac cgttcacaac agcatcactt tgaacaacat ttgttaacgg attatttggc
    10741 aattcggtta gttgtcgaac aattgctagt tggtgatgag tttaagtcag tcactaagga
    10801 ttgtgaaagt agatcggaaa attggtttaa gcaaactgtt gcatcatggt gttactacag
    10861 tgatatgcct agcgatgtat tactacaaca tgacgtcaat gaaattcaaa cgtttattca
    10921 ttttgcagca actatgaata aaaatgtatt taaaaattta tggttgattg cctgggaaat
    10981 gacatacgaa tctcagttaa aacaaaaaat taaagcaggt catgagagtt tggcgggcgc
    11041 attagatgca attcaagtaa atgtttcgga aaatgacaac gctaatcagt cacattcagt
    11101 atcgttaaat gccacacaag cagttgatga aaataatagc gagctaaatc aggtgggcac
    11161 atcaacgaaa gcgcaaattg cattttgtat agatgttcgt tcagaaccat ttcgcagaca
    11221 tatcgaagca gcagggccct ttgaaacgat tggtattgca ggtttctttg gattacctat
    11281 tcaaaaagat gccgtagacg aacaattcaa acatgattca ttacctgtca tggtaccgcc
    11341 ggcatatcgc attaaagagt ttgcagaccg ttatgatatg aatgtttatc gacaacagca
    11401 acagacaatg tcatcgatgt tttacacatt taaattgatg aaaaataatg tcatgcctag
    11461 tctgttattg cctgaattaa gtggaccatt tttaagcttg agtaccattg tcaatacgat
    11521 tatgcctaga aaaagtcgcg tgtctttaca gaaaataaaa caaaaatggt tgaagaagcc
    11581 tgaaacaaag ttaacgattg atcgtgagtt tgacagaaca tcagacttac ctgttggatt
    11641 tactgagcaa gagcaaattg atttcgcgtt acaagcgttg aaattgatgg atttaaccga
    11701 agcatttgcg ccgttcgttg tgttagcagg tcatgctagt cattcccaca ataatccaca
    11761 tcatgcatca cttgaatgtg gggcttgtgg tggtgcatca agtggcttta atgctaagtt
    11821 attagcgatg atatgtaatc gtccaaatgt cagacaagga ttaaaacaat caggtgtgta
    11881 tattccagag acaactgttt ttgcggcagc agaacatcat acgtctactg atacgttggc
    11941 atgggtatat gtgccagaca cattatcttc tattgcttta gatgcatatg aatcattgaa
    12001 tgacgcgatg ccgatgattt ctgaacacgc gaatcgcgaa cgtttggaca aactgccaac
    12061 gattggtcgt gtgaatcatc cagtggaaga agcgcagagg tttgcgagtg attggagtga
    12121 ggtacgtcca gaatggggct tggctaaaaa tgcatcattt ataattggac gacgccaatt
    12181 aacaaaaggc attgatttag aagggcggac atttttacac aattatgatt ggcgtaaaga
    12241 taaagatggc aaattgttaa atactatcat ttctggtccg gcgcttgtgg cacaatggat
    12301 taatttacaa tattatgcgt cgacagttgc gccgcatttt tacggaagtg ggaataaagc
    12361 gacacaaacc gtcacgtcag gtgttggtgt catgcaaggt aatgcgagtg atctgatgta
    12421 tggcttatca tggcaatctg ttatggctgc tgatcgaacg atgtatcatt cgccaattcg
    12481 tttgcttgtc gttgttcagg cacccgacta tgttgtagca agactactcg cgaataatga
    12541 ccatttcgct aggaaggtgt ctaatcattg gctgcgttta atgagcgtta atgaggaagg
    12601 gcgttttaaa agttggattt aacgatgata tgttgcgatg cttagtaact aatatatcat
    12661 catatctaca atgttctagc ttcttaaaat atactaaaaa tgataaactt aatgtaatag
    12721 ttgatgtcgt gataacgata caaagatgaa gtaatcaatt ttaatattat tggagttagt
    12781 gatatgaaaa gaacgaaagg tgaaatcgaa gctgaaatca gtaaagccat tacgcaatgg
    12841 gaaaaagatt tccttggcag aggttccttg tcagttaaat cagacatttt aagagatatg
    12901 gtgattatta gtttacaagg tatcttaacg ccagctgaat atcgtgtgtg tagtacgaat
    12961 gaaggattac taaatattaa acgaacacgt tctgaattag ttgaatccgg tgagcaagat
    13021 ttgaatgata tcattcttaa aattacaggt atcaaagtga tgagcttcca cagtgattta
    13081 agtacagtta caggtgaacg tattatcgta ttcaaacttg aggataattt ggaaaagcat
    13141 atttaagaag gttaggtatg cttctgctgg cttattaaag ctatagaagt atgtttggtt
    13201 aaacagcgca acgccatata tttgggtgtg ctgtttttct attatatgat gtgtttatta
    13261 cgtggatgaa tgcgtgggtc tgaatatagc ccgtgaaagg cgtatgtcat aaaatttgcg
    13321 aatggtgaca agtcgacgca agcatatttg ttgcgagaca tgcccactat ccttttacat
    13381 aaagagtata tgtgtgacgt aggcatataa tcgataaagt attcctaaaa aattaggtat
    13441 aatgaccatt gttgcaataa gtttttcggc cttgaaaccg ataacgcata gtataatagg
    13501 aataaaatat aatacaaata tccagtaaat caaatttgaa aacgatggat gaataggggt
    13561 aaagaatcta actaatgtaa tgattaacgc tatatagaca aaaataactc tattgatacg
    13621 ccttaccccc tctgtataat aaatatagat cgtactaaat tgaaaatagc aaaatattgt
    13681 tatttcaatt atacaatgtt ttatttgcaa tatacataac aatatgaact ttataatttt
    13741 gatattgtcg gtaaagtgaa atttgtggca cattgatatt atggataaca tttgtaaagt
    13801 gaaaatgatg atacgaggag attgtaagat gatagataaa aaattaacat caccgaaaat
    13861 gacagtgcct ttatttttaa tcgcgctgat tgtatttata ggtatgtttt acagtgtagt
    13921 gacaaatcaa gaatggctta aaaatataga tatgggatca ttaacatggt ttacagatta
    13981 tttcggtgag ccacaacgtc agtatgttaa caatttattt aattactata tgacgtttag
    14041 tgcggaaatt ggagatgtca aaggtgtcgt gttgatttcc attatcgtca caatcatact
    14101 gtttattaaa cagaggcatt tagcggtttg gtttgtgaca tatttggttt caggtgtcat
    14161 catgaacaaa ttaattaaag atactgtttt acgtccaaga ccatataatc atttagccgt
    14221 tgatacaggc ttttcatttc caagtggaca ttccaacgcc agcacattat tatatttcgc
    14281 cttaatgatc ataattattt cacttgctgc taagacaata acaaaagtgt tgagtgcgct
    14341 agttatggga atattatggc ttagcatatt attttgtcgt ctttattttc atgcgcatta
    14401 cttttcagat gtcattggcg gcacgtcact agcaatcatt tgggtagcgt tatttttaat
    14461 ggtataccca tactttatta atcatcgacg acaacgcgtt tagcaaacat ctttagacat
    14521 agtggaggta tatagaaaaa tgagaattaa aacaccgagt ccatcttatt taaaaggcac
    14581 aaatggacat gcgatattat tattacattc atttacaggt acaaatcggg atgtgaagca
    14641 tcttgcagct gagttaaatg accaaggatt tagttgttat gcaccgaatt atccaggtca
    14701 tggcttactg ttgaaagatt tcatgacata taatgtagat gattggtggg aagaagttga
    14761 gaaagcttac caatttttag tcaatgaagg ttatgaatct atcagtgcaa cgggtgtgtc
    14821 tttaggtgga ttaatgacat taaaattggc acaacactat cctttgaaac gtatcgctgt
    14881 catgtcagca ccaaaggaaa agagtgatga tggtttaata gaacatttag tttattatag
    14941 tcaacgcatg tcggatattt taaatttaga tcagcaagca tcgagtgcgc aattagcagc
    15001 aattgatgat tatgaaggtg aaattacgaa gtttcaacat tttattgatg atatcatgac
    15061 aaatttaaat gttattaaaa tgccagctaa tatattattt ggtggtaaag atgcgccatc
    15121 ctatgaaaca agtgcacact ttatttatga acatttagga tcagtagaca aagaattaaa
    15181 tggtctgaag gattcgcatc atttaatgac gcatggagaa ggcagagata ttttagaaga
    15241 aaatgttatt cgctttttca atgctttaac ataattgaag attttaaaaa tcaacacttg
    15301 agtgacgatg ttattatatt tgtcatttga gtgttttttg tggcgtgata gtatagggaa
    15361 cttagctaaa gttaatttgt agtgtaatgt cgtggaaata tgatggagtt ataccagaag
    15421 ttgatttgta ttgtaatgtc gtggaaattt tatgggcatt gcacctgaag ttgttatgtg
    15481 gcgagatgtt ggggatatag atggtgttgt atgtggtgag atgttggcgc acgtatacag
    15541 cgttgaacca gaaattttaa tgtagcgtaa tgacaacaca ttacaagagt tgactatatt
    15601 atgataaata caggcgctac cgtattaatg aaacaatttt gatgcaggtg tattgcatgt
    15661 tatttacata catgttagag tgttacttaa acacgtagac gctttaacgc tttaaaggag
    15721 atgtgaatga tgaaaagaca acaatcacaa tggaagtcat caactggatt tattttagct
    15781 agtgcgggtt ctgcaatcgg tcttggtgcc atgtggaaat tcccatatat ggcagggatt
    15841 tatggcggcg gtgcctttct agctatgttc ttaatattca ccatttttgt tgggttgcca
    15901 ttactcatta tggaattcac tgttgggaaa atgggacgga catatacaac acaaatatat
    15961 agtaaattaa ctggtaaaaa atggctcaat atcattggct ggaacggtaa tttggcagtg
    16021 tttattttat ttggcttcta tagtgttatc ggtggttgga ttgtcattta catcggacaa
    16081 gtattatggc aattagttat atttcaacgc atcaatcatc tccaagaaat gaattttgaa
    16141 gcggtaatat caaatccttg gttaactgtt ctagggcaag gtatattcat attcgctacg
    16201 atgattattg tcatgttagg tgttgaaaaa ggattagaaa aggcatcgaa agttatgatg
    16261 ccattgctgt ttgtcttttt aatcatcatt gtgattaagt ctttaacatt agatggtgcc
    16321 ttagaaggtg tgaaatttat tttacaacca agagtatcag agattactgc tgacggtatc
    16381 ttgtttgcgc taggccaatc attctttacg ttatcattag gaactacagg tatgattact
    16441 tatgcgagtt atgcctctaa agacatgacg attaagtcat cagctatttc tatcgttgtt
    16501 atgaatatct ttgtatctgt attggcaggt ctagctatat ttccggcgtt acatagtttt
    16561 ggctatgaac cacaagaagg gcctggatta ttatttaaag tactaccaat ggtctttagt
    16621 caaatgcatc taggcacatt attctatttg ggattcttag tgctgttctt atttgcggct
    16681 ttaacgtcat ctatttcttt attagaatta aatgtttcta acttcacgaa gaatgacaat
    16741 acaaaacgta aaaaagtcgc agtgattggt agtattttag tatttatcat tagcattcca
    16801 gcaaccttat cttttggtat cttaaaagat gtaagattcg gtgcgggaac gatttttgat
    16861 aatatggatt tcatcgtttc gaatgtattg atgccattag gcgcattagg tactacgctt
    16921 gtcgtaggac aattattaga taaaaaatta ttacaacaat attttggtaa agatcgattt
    16981 aggttattca gtggttggta ttacttaatt aagtatgcga tgcctgtcgt tattatttta
    17041 gtctttatcg tgcaattatt tagttaatat atagaagcat ctgacacaca agtatttgtg
    17101 ttgctcaggt gtttttttgt tttgaattga aaatttagta ctggttctga attaatcgaa
    17161 acttagataa ttgcaaaata acaaaatgtc gtttataatt cttatctgaa aagtaagaat
    17221 taaaattgag acatgaagaa aggtttcatt catttgatcg gcatgtcaga tgagggtgca
    17281 tctatgatta cttatgattt aattggcaat acaccattag tactgttaga acattatagt
    17341 gatgataaag ttaaaattta tgccaagctt gaacaatgga atcctggagg cagtgttaaa
    17401 gacagactcg ggaaatattt agtagagaag gcaattcaag aagggcgtgt gcgtgcaggt
    17461 caaactattg ttgaagcgac tgctggtaat acaggcatag ggttagttat tgcagcgaat
    17521 agacatcatt tgaaatgtaa gatctttgcg ccgtatggtt tttcagaaga aaagattaat
    17581 attatgatag cgcttggtgc agatgtttca aggacgagtc agtctgaagg tatgcatggg
    17641 gcacaattag ctgcacgttc ctatgctgaa aaatatggtg ccgtttatat gaatcaattt
    17701 gaatccgaac ataatccgga tacatatttt catacattgg gacccgaatt gacttcagca
    17761 ttacagcaaa ttgattattt tgtggctggt attggctctg gcggtacatt tacaggtacc
    17821 gcacgttatt taaagcaaca tcacgtgcaa tgttatgccg ttgagccaga agggtccgtg
    17881 ttaaatggag ggccagctca tgcacatgac actgaaggta tcggttctga gaaatggccg
    17941 atatttttag agagacgtct tgtagatggg atatttacga ttaaagatca agatgccttt
    18001 cgaaatgtca aaagtttggc tataaatgaa gggttgttag taggcagttc ttcaggtgca
    18061 gcattacaag gtgcattgaa tttaaaagcg caactaactg aaggtacgat tgttgtcgta
    18121 tttccagatg gtagtgatcg ctatatgtct aaacaaatat ttgaatatga ggagaataat
    18181 aatgaataag aaaacgaaat taattcacgg tgggcacaca acagacgatt atacaggtgc
    18241 cgttacaaca ccaatttatc aaacaagtac atatttacaa gatgatattg gtgatttacg
    18301 tcaaggatat gaatattctc gtactgcgaa tccaacaaga agttctgtag aaagcgttat
    18361 tgcgacatta gaaaatggca aacatggctt tgcatttagt tcaggtgttg cagcaatcag
    18421 tgcagttgtt atgctgttgg acaaaggtga tcatattatt ttaaattcag atgtatacgg
    18481 cggtacttat cgcgcattga caaaagtatt tacacgattt ggcattgaag tggattttgt
    18541 agatacaact catacagatt caattgtaca agcgatacgc ccaacaacaa agatgttgtt
    18601 tattgaaaca ccttctaatc cattattacg tgttactgac attaaaaagt ctgctgaaat
    18661 tgcgaaagaa cacggtttga tttcagtcgt tgataacaca tttatgacac cttattatca
    18721 gaatccatta gatttaggca tcgatattgt cttgcattct gcaacgaaat atttaggtgg
    18781 acatagtgat gtcgttgctg gtttagttgc aacatcggat gacaagcttg cagaacgttt
    18841 agcatttatt tcaaattcaa caggtggcat tttaggacct caagatagct atttacttgt
    18901 gaggggtatt aaaacattag gcttacgtat ggaacaaatt aatcgcagtg ttattgaaat
    18961 tattaaaatg ttacaagcac atccagctgt gcaacaagtg ttccatccaa gtattgaaag
    19021 tcatttaaat catgatgtcc atatggctca agcggatggc catacaggtg tgattgcatt
    19081 tgaagtgaaa aatacagaaa gtgccaaaca attgattaaa gcaacatcgt attacacatt
    19141 agctgaaagt ttaggtgcag tggaaagttt aatttcagta cctgcattga tgacacatgc
    19201 atccattcca gcagatattc gagctaaaga aggtattaca gacggacttg taagaatttc
    19261 tgtaggtatt gaagatactg aagatttagt cgatgattta aaacaagcac tagatacgct
    19321 ataaataata gcagcactag catatatttt gagttcaacg tttgttgttc ttgaatatgc
    19381 tagtgctttt ttttgtgcga tgttaatcat tcgtgattga gttatggagg tttggtggac
    19441 atttttatgg atttaatttg ataaatgtca tagtagtctc acaattcgtc attgtctcat
    19501 gaattactta ttttttaatt ttttagaaaa ttcggcaaat tgtattgacg atatatgcaa
    19561 ttttacatat aatgctctta ttcctagtgg attaataaga atttgtagga gggggctttg
    19621 catgattgag tttcgacagg taagtaagac ctttaataag aaaaagcaaa caatagatgc
    19681 tttgaaggac gtatcattta cggtcaatcg caatgatatt tttggtgtga ttggatatag
    19741 tggtgcagga aaaagtacgt tggtaagact cgtgaatcat cttgaagctg cctcgaatgg
    19801 acaagtgatt gtagatggac atgatattac gaattatagc gataaaatga tgagggacat
    19861 taagaaagat atcggtataa tatttcagca tttcaattta ttaaattcag ctacggtatt
    19921 taaaaatgta gcaatgccac tcattttaag taagaaaagc aaaacagaaa ttaagcaacg
    19981 agtaacggaa atgcttgaat ttgtaggatt gagtgataaa aaagaccaat ttcctgatga
    20041 attatctggt gggcagaagc aaagggtggc tattgcaaga gcgcttgtta ctaatccgaa
    20101 aatactccta tgcgatgaag caacaagcgc attggatcca gcaacgactg cttcgatatt
    20161 gacgttatta aagaatgtca atcaaacctt tggcattaca attatgatga ttacacatga
    20221 aatgcgcgtt attaaagaca tttgtaatcg tgttgctgta atggaaaagg gtcaagtggt
    20281 tgaaacagga actgttaaag aggtgtttag tcatcctaaa acgacgattg ctcaaaattt
    20341 tgtgtctaca gttatacaga ctgagccaag tacatcattg attcgtcgat tgaatgacga
    20401 acaagttggc gattttaaag attataaaat cttcgtcgag gaaactcagg tgacacaacc
    20461 gattataaat gacttgattc aaatttgtgg cagagaggtt aaaattttat tttcatctat
    20521 gtcagaaata caaggtaaca ccgtatgtta tatgtggctt cgatttaata tagatcaaca
    20581 atttgatgac acggcaataa atcaatattt caaagagaaa aatattcaat ttgaggaggt
    20641 gcattaacat atgtttggtt ctgatttaga cagtgcacag ttattacaag cattgtacga
    20701 aacgttatat atggtatcta ttgctttatt tttaggagca gtgattggta ttccattagg
    20761 tgtcttattg gtaattactc gaaaacaagg catatggccg aatatagtga tacatcaagt
    20821 tttaaatcct ataatcaata ttttaaggtc actaccattt attattttgt taattgcgat
    20881 tgtgccattc acaaaattag tagtaggtac ttcaattggt acgactgctg ccatcgtgcc
    20941 tttaacagta tatgtggcac cttacattgc acgacttgtt gaaaactcat tattagaagt
    21001 agacgagggg attattgaag cggcgaaagc gatgggcgct tcaccactac aaatcattag
    21061 atatttttta attcctgaag cgttaggttc attagtatta gcaattacca ctgcgattat
    21121 tggacttatt ggaagtacgg cgatggcagg agctgttggc ggtggtggta taggagactt
    21181 agctttagtg tatggttatc aaagatttga tacgacggtc attattatta ccgttattgt
    21241 attagtcatt attgtccaag tgattcaaac gctagggaat gtcctagcta gattcatacg
    21301 tagacattaa tgatatatag tgaagatttt gaaaggaatt gataaaatga aaagattgat
    21361 tgggttagtt atcgtagcac ttgtattatt agcagcgtgt ggtagtaaca atgataaaaa
    21421 agtaacaatt ggtgtcgcat caaatgacac taaggcttgg gagaaggtta aagaattagc
    21481 taaaaaagat gatattgatg tggagattaa gcacttctcc gattacaatt taccgaataa
    21541 agcattaaac gatggtgata ttgatatgaa tgcattccaa cattttgcat ttttagatca
    21601 gtataagaag gcacataaag gaacaaagat ttcagcattg agtacaacag ttttagcacc
    21661 gttgggcatt tactcagata aaattaaaga tgtcaaaaag gttaaagatg gtgctaaagt
    21721 tgtcattcca aatgatgtat caaaccaagc acgtgcactt aaactattag aagcagctgg
    21781 tttaataaaa ctgaaaaaag atttcggatt agcaggtaca gtgaaagata taacgtcaaa
    21841 tccaaaacat ttaaaaatta ctgcagtaga tgcacaacaa actgcacgtg ctttatctga
    21901 tgtcgatatt gcagttatta ataacggtgt agcaactaaa gcgggtaaag atcctaaaaa
    21961 tgatccgata tttttagaaa aatcaaattc agatgctgta aagccatata ttaatattgt
    22021 tgcagttaat gacaaagact tggataacaa aacatatgct aaaatcgtag aattgtatca
    22081 ttcaaaagaa gctcaaaaag cgttgcagga agatgtcaaa gatggagaga aacctgttaa
    22141 tttatctaaa gatgagatta aggcaataga aacgtcatta gcaaaataga ttatattgcg
    22201 tcctacaagc aaagttcatg cttatgtttg tagggcgtta ttgtttgaca ataaaattat
    22261 ttccaataga gaaagggatt gtaatcattt tatagtgaaa tattatgaaa ttgtaataat
    22321 ttagatattg taaaatctaa taagttgtaa taattttaag gggtaattat aaaatttgat
    22381 gatacagtat atgatttttt tgtaatcata atgtcatcaa acatcaacct attatacata
    22441 ataaaatcgt ataatgatgt agtattcata aattcggata aaagaatgtt aggaaagtta
    22501 agcaagagga ggattttaaa gtgcaaaaaa aagtaattgc agctattatt gggacaagcg
    22561 cgattagcgc tgttgcggca actcaagcaa atgcggctac aactcacaca gtaaaaccgg
    22621 gtgaatcagt gtgggcaatt tcaaataagt atgggatttc gattgctaaa ttaaagtcat
    22681 taaacaattt aacatctaat ctaattttcc caaaccaagt actaaaagta tctggctcaa
    22741 gtaattctac gagtaatagt agccgtccat caacgaactc aggtggcgga tcatactaca
    22801 cagtacaagc aggcgactca ttatcattaa tcgcatcaaa atatggtaca acttaccaaa
    22861 acattatgcg acttaatggt ttaaataatt tctttattta tccaggtcaa aaattaaaag
    22921 tatcaggtac tgctagctca agtaacgctg cgagcaatag tagccgtcca tcaacgaact
    22981 caggcggtgg atcatactac acagtacaag caggtgactc attgtcatta atcgcatcaa
    23041 aatatggtac aacttatcaa aaaattatga gcttaaatgg cttaaataat ttctttattt
    23101 atccgggtca aaaattgaaa gtaactggta atgcatctac gaactcagga tctgcaacaa
    23161 ctacgaatag aggttacaat acaccagtat tcagtcacca aaacttatat acatggggtc
    23221 aatgtacata tcatgtattt aatcgtcgtg ctgaaattgg taaaggtatt agtacttatt
    23281 ggtggaatgc taataactgg gataacgcag cggcagcaga tggttacact atcgacaata
    23341 gacctactgt aggttctatc gctcaaacag atgtaggtta ttatggtcat gttatgtttg
    23401 tagaacgtgt aaataacgat ggtagtattt tagtttcaga aatgaactat tcagctgcac
    23461 caggtatttt aacttacaga acggtaccag cttaccaagt aaataattat agatatattc
    23521 actaaagtct tacgtatata aatatataat gaattcctat tacatttcac aagctgaaaa
    23581 gttggattga atcctaaact ttatggcggg gctatcaaag tcgtgattgt tgtaatagga
    23641 atttttgtat aagaaaaagg attggtcgaa tgaacttcat gttctatgtt cgaccaatcc
    23701 ttttctgaat tagtattcat gctttacttt gcgaatgctt gtaaataatc tagcaccgtt
    23761 tgttattaaa gtaacaactg ccactgcttt tttgaactta cgtggtgact taattgaaat
    23821 gtaaaagtct aacacggttc ctacagtgct taaacctaat gaaagatata ctaatttttt
    23881 atttttagca tgatatttat agccattgta gccgtcgact atgaaacctg cgacatttag
    23941 taaacttgat aaacgttgtg atttggaacg ttttgccata ataataattc cccctagtat
    24001 cttctgttta tatgaaaaat tgggtgcgct gaattgctaa cgttttgcgc tataactact
    24061 catatatgat aacataattg tacagtataa ttttaaaaat tgatttcaca aagttggggt
    24121 gtcaaagatg attaaatgtg tctgtttagt tgaagaaaca gctgataaaa tattacttgt
    24181 tcaagtaagg aatcgcgaaa agtattattt cccaggtggt aaaatagaag aaggagaatc
    24241 acaagtacac gcgctgttaa gagaagtaaa agaagaatta aatttaacat taacaatgga
    24301 tgaaattgaa tatatcggga caattgtagg tcctgcatat ccacaacagg atatgttaac
    24361 tgagttaaat ggatttcgcg cattaaccaa aatcgattgg gaaaacgtaa ctatcaataa
    24421 tgaaattacg gatatacgct ggattgataa agataatgat gcgttgattg cgcctgctgt
    24481 caaagtttgg attgaaacgt atggtggtaa acatgacaaa taatgacacc atcatgttac
    24541 gacattatgt cccacaagat tattcgatgt tagaagcttt tcaattaagt gaaagtgatt
    24601 tgaagtttgt taaaacgcca gaggaaaata ttacagctgc aatgtctaat aatgaaaggt
    24661 atcccatcgt tgtaatggat ggcaggcaat gtgtggcctt ttttacatta catcgtggaa
    24721 aaggggtcgc accatttagc gataaccaag atgcagcatt tttcaggtca tttagtgttg
    24781 atcaacgtta tcgtaataga ggaataggta aagtggtaat ggaaaaattg gcgtcattta
    24841 tcacttcaac atttcaggat attaatgaga ttgtgttaac ggttaatact gacaatccac
    24901 atgccatggc actttatcgc caacaaggat atcaatatat gggagatagt atgttcgtcg
    24961 gaagacctgt tcatattatg gcgttaacta taaaataaat taaatttaaa agcatcttta
    25021 ctcatcgtcg accacaacaa ttaatgatga ataaaggtgc tttttgttat agatcaccgg
    25081 acaatttact atagtaaaaa gcgacctagt gaacaattga catatatcca caggtcgctt
    25141 aacttaagtt atattgctag ttgcgattaa ttgatagact catcattttt gcgctgtcga
    25201 gatggtcttt ttattaaaaa tgccgtaatc caagccgtaa tcggaatact gattgcaacg
    25261 gcaataccgc ctaaaataat agaaataaat tcttgggcaa atattttcga gtttataata
    25321 tgaccaaatg aatatttaag tttgaaaaac caaataaata aagcaagttg gccaccaaaa
    25381 aaggcaaggt aaatcgtgtt cgcagatgtc gctaaaattt ctctaccaac acgcatgcca
    25441 gattggaata attcgtattg cgtaagtgtt ggattcactt gatgcaattc ataaatgggt
    25501 gaactaatgg taatcgttaa atctatcaca gctgcaataa cagcaagaat aatagtgaac
    25561 accataaatt gaaccatatc aatgccaata ttcattgaat acacatatgt ttcatcttgt
    25621 tgttcggttg aaaagccttg tagatgaccg aagtagaccg ataaataaat gagtgtaatc
    25681 aacaatattg ttgtaacgat agtgctgata aatgcagctt gtgttttaac attgtaacta
    25741 ttgagtacga ataaattaca agcgccaata ataatgcaga aaaagaatgt gacgacataa
    25801 atcggtacgc caaaaataat caacacaata ctaataatta aaatagcgaa atttaaaaat
    25861 agggttaaat aagagatgaa tcccttttta cctccgaaaa ttatcatcag aaagaggagc
    25921 aataccgcca atataaatac agcattcatt gtttcgccct ccttaatgtt tcaaatattt
    25981 ccataaacaa tattgtgata ggaattgtaa gtacgatacc tataccgcct gttagtgcgc
    26041 gcgcaatttc taacgaccaa ttcatcgaaa tagtataagt cacagtattt gcatttttta
    26101 aaaagattaa aaacataggt agtgcaccgg ataaatatga gaataataag atgttagtca
    26161 ttgttcccat aatatcttgg ccgatgtttc gcccagcaag cgcccatctc ctcattgaaa
    26221 tgtgtggcgt acgctgtaaa atttcatgca taccactagc aattgtaatt gcaacatcca
    26281 taatagcgcc aagtgaacct attaacactg aggctaggaa gatatctttc ggtggtaatg
    26341 ataaaaagtt catcgtttca tatttaatgc ctttaccatc tgtcatatat atgattaatt
    26401 ctgttaaacc tatactcaaa aaagttccga taattgtact ggctatggta atgagtgtac
    26461 gcatatgcca gcctgtaacg agcaataaag tgagtattgt tgaacagatc atggcaatgg
    26521 tcatgagtaa gaataaatta atattgctat gttgaatatg aatgtaaatt gcgattaata
    26581 tggcaataga attcaagatt aacgataaaa tcgattgcag tccgactttg cgaccaacca
    26641 ataatacagt taataagaac aaaccagtga tgataaccgt taaggtatca cgcttctttt
    26701 ctataatata agcatcactc ggcttgttag aaatatgtaa taatactttt tcgtgtgtgc
    26761 gaaatgcctc agaatctgct tgcgatttga cgtactgatg attaatcgtc gtcgtttctc
    26821 cagcaaattg accatttaat attttgactt ttaattgatt tttatattta atatcacgat
    26881 tattttgtgc atcttttgta ggtgtcgaag aaacatgttt gacatctata atttgaccaa
    26941 ttggtttgtt gtaaaagttc tcattattga atgtaaataa aatagcacca atgaatgcga
    27001 tgcagaacaa acctaaaatt atattaaatg gctttgtaaa taaatttcta tatttcaaaa
    27061 acaaaacccc aattctatga atgaattaat atggtgatta tacgccctta attttttatt
    27121 ttcaaagata ttactgctaa gtgtaaaacg aaaatcatca ttgatagcat cgaattactt
    27181 aatggaatgt agacgtttta gtcattaatt gctgaataag tgttaataat atgccaatat
    27241 cactctttgt ataaggctcc tttgtaatag cacatatcgt tctttttaat tcagtatgat
    27301 ctaattttat atctatccat gatttagatt ctggtaaatg tatattttgt gatgaaatga
    27361 tgtaaccttc tttttgacga aggagatact gcgcaagtgg ttggctactg attgtgtata
    27421 catctgattt agtaatcttg cgcaattgtt tttttacagt ttcggcaaat ggtgccaagc
    27481 aataaatatg actatgctca aactgaatta atggtgggtg tgtcgccatc gtaattggat
    27541 cgtctgaagg cgcatataaa tgatagtgct cttcgaataa aggtagcata tgtaattgtt
    27601 tgtgtttacg tatttctggt gtaagttccg tgaaaccaat gtctatattc ccatttaata
    27661 cgctatttat aattgtgtca tgttctaata agctcggtat gacatgtgta tcattttgta
    27721 aatgaaacgt ttggataagt ggtagtaaca tgtgggatac gtcactctca tcatagccaa
    27781 tgtagatact tttattttta gttaatccat ggctttgaaa ttgttcaatc gtgctatcta
    27841 aatgttcaat aatacgcaga gcttcattaa ataataattt cccttcagat gtgagcgtaa
    27901 tattgcgtcc ttgcttttta aataaagaca cattaagttc ttgttctaat aatgtaattt
    27961 gacggcttat cgctgattga gcaatgttta gttcaagtgc tgtttcggag atatgttctc
    28021 ttttagcgac ctcgataaaa tatcttaatt gtttaatttc catagcgata taggcacctc
    28081 caaaaatgag tgttttgtaa ctattatagc aatattattg ataaatgttc tattttttag
    28141 atgaatatct tctattttat atattgaaca gataaatttt ttagattata gtaattatca
    28201 ttaataacta atatcagaat attctaaaaa aaggggtgtg catcatgcac aatgagaaat
    28261 taattaaagg cttatatgac tatcgtgagg aacatgatgc gtgtggtatt ggtttttatg
    28321 cgaatatgga taataaaagg tctcacgaca tcattgataa atcgcttgaa atgttgcgac
    28381 gcttagatca taggggcggg gtcggcgcag atggcatcac tggtgatggc gcaggtatta
    28441 tgactgaaat accttttgca tttttcaaac aacatgtaac ggactttgat atcccaggtg
    28501 aaggtgaata tgccgtgggg ttattttttt ccaaagaacg cattttaggt tctgaacatg
    28561 aagtagtttt taaaaaatat tttgaaggcg aagggttatc aattcttggt tatcgtaatg
    28621 taccagttaa taaagatgcc attgctaaac atgtagcaga tacgatgcca gtcattcaac
    28681 aagtgtttat tgatattagg gacattgaag atgttgaaaa gcgtttgttt ttagcgagaa
    28741 aacaattaga gttctattcg actcagtgcg atttagaatt gtattttacg agcttatcac
    28801 gcaaaacaat tgtatataaa ggttggttac gatcagacca aattaaaaaa ctatatacag
    28861 atttatcgga tgatttatat caatcaaagc tagggttagt gcattcgaga tttagtacga
    28921 atacattccc gagttggaaa agggcacatc ctaaccgtat gttaatgcat aatggtgaga
    28981 ttaacacgat taaaggtaat gtaaactgga tgcgagcacg ccaacataaa ttaatagaaa
    29041 cattatttgg cgaggatcaa cataaagtgt ttcaaattgt cgatgaggat ggtagtgact
    29101 ctgctattgt agataatgcg ctagagttct tatcgttagc catggagcca gaaaaggcag
    29161 cgatgctact catacctgaa ccttggttat acaatgaagc gaatgatgca aatgtacgtg
    29221 cgttttatga attttatagt tatttaatgg aaccgtggga tggtcctaca atgatttcgt
    29281 tctgtaacgg tgacaaactt ggcgcgctta cagatagaaa tggattacgt ccaggtcgtt
    29341 atacgattac taaagataac tttattgtct tttcatctga agtgggtgtt gtggacgtac
    29401 ctgaaagtaa tgttgctttt aaaggtcaat tgaatcctgg aaagttattg cttgttgatt
    29461 ttaaacagaa taaagtcatt gaaaataatg atttaaaagg tgcgattgct ggagaattac
    29521 catataaagc gtggattgat aaccataaag ttgactttga ttttgaaaat atacaatatc
    29581 aagattcgca atggaaagat gagacgttat ttaaattaca acgtcagttt gcatacacga
    29641 aagaagagat tcataagtat attcaggaac ttgtagaagg taagaaggat cctatcggtg
    29701 caatgggata tgatgcgcca attgcagtgt tgaacgagcg accagaatca ctatttaatt
    29761 actttaaaca gctgtttgca caagttacga atccaccaat tgatgcgtat cgtgaaaaaa
    29821 tcgtaacgag tgaactttct tatttaggtg gcgaaggtaa cttactagca cctgacgaaa
    29881 cggttttaga tcgtattcaa ttgaaaaggc cggtattgaa tgaatcacac ttagcagcga
    29941 ttgatcagga acattttaaa ttaacttatt tatcaacggt atatgaaggg gatttggaag
    30001 atgcgttaga agcattaggc cgagaagcag tgaatgctgt aaagcaaggc gctcaaattc
    30061 tagtgttaga tgatagtgga ttagttgata gcaatggctt tgcaatgccg atgttactcg
    30121 caataagtca tgtgcatcaa ttacttatta aagcagattt acgtatgtct acaagtttag
    30181 tcgctaaatc tggtgagaca cgagaagtgc atcatgttgc ttgtttactc gcatatggcg
    30241 cgaatgcaat tgtgccatac ctagcgcaac gtacagttga acaactgaca ttgacagaag
    30301 ggttacaagg caccgttgtc gataatgtta agatatatac ggatgtattg tcagaaggtg
    30361 tcattaaagt aatggctaag atgggaattt cgacagtgca aagttatcaa ggggcacaaa
    30421 tatttgaagc gattggcttg tctcatgatg tgattgatcg ttattttact gggacacagt
    30481 ctaagttatc tggtatttcg attgatcaaa ttgatgctga aaataaagca cgtcaacaaa
    30541 gtgatgataa ttatcttgca tcaggtagta cattccaatg gagacaacaa ggtcaacatc
    30601 atgcttttaa tccggaatct attttcttat tgcagcacgc atgtaaagaa aatgactatg
    30661 cgcaatttaa agcatactct gaagcggtga acaaaaatag aacagatcac attagacatt
    30721 tacttgaatt taaagcatgt acaccgattg acatcgacca agttgaaccg gtaagtgaca
    30781 ttgtcaaacg ctttaataca ggggcgatga gttatggatc gatttcagcg gaagcacatg
    30841 aaacgttagc acaagccatg aaccaattag gtggaaagag taatagtggt gaaggtggcg
    30901 aagatgcaaa acgttatgaa gtacaagttg atggaagcaa caaagtaagt gcgattaaac
    30961 aagttgcttc tgggcgtttt ggtgtaacta gtgattattt acaacatgcc aaagaaattc
    31021 aaattaaagt tgcgcaaggt gcaaagcctg gtgaaggtgg tcaattacct ggtactaagg
    31081 tatatccgtg gattgcgaag acaagagggt caacgccagg tatcggtctg atttcaccac
    31141 cgccacatca tgatatttat tcaatagaag atttagcgca actgatacat gatttgaaaa
    31201 atgcgaataa agatgcagat atcgcggtaa aattagtttc gaaaacaggt gttggtacca
    31261 ttgcatctgg ggtggcaaaa gcatttgcag ataaaattgt catcagtggt tacgatggtg
    31321 gtacaggggc ttcacctaaa acgagtattc agcatgccgg tgttccttgg gagattggtt
    31381 tagcagaaac acatcaaaca ttaaaactaa atgacttaag aagtcgtgtt aagttagaaa
    31441 cagacggtaa gttattaact ggtaaagatg tagcgtacgc atgtgcgctt ggagcggaag
    31501 aatttggatt tgcaactgca ccattagtgg tgttgggctg tattatgatg cgtgtatgcc
    31561 ataaagatac atgtccagta ggagttgcaa ctcaaaacaa agatttacgt gctttatata
    31621 gaggtaaagc acatcatgtt gttaatttta tgcattttat tgcacaagaa ttaagagaaa
    31681 ttttagcatc tttaggtttg aaacgtgtag aagacttagt tggaagaact gatttattac
    31741 aacgatcatc aacattaaaa gcgaatagca aagcggctag tattgatgtt gaaaaactgt
    31801 tatgtccttt cgatgggcca aacacaaaag aaattcaaca aaatcataat cttgagcatg
    31861 gatttgattt aacaaattta tatgaagtaa cgaagccata tattgctgaa ggacgtcgct
    31921 atacaggtag ctttacagta aataatgaac aacgtgatgt aggggttatt acaggtagtg
    31981 agatttcgaa acaatatgga gaagcaggac ttcctgaaaa tacaattaat gtttatacga
    32041 atggtcatgc tggtcaaagt cttgcagcat atgcaccgaa aggcttaatg attcatcata
    32101 ctggagatgc gaatgactat gttggtaaag gattatctgg tggtacggtc attgtcaaag
    32161 caccttttga agaacgacaa aatgaaatta ttgctggtaa cgtctcattc tatggtgcga
    32221 caggtggtaa ggcatttatt aacggtagtg caggagaaag attctgtatt agaaatagtg
    32281 gtgtagatgt tgtcgttgaa ggtatcggtg accatggatt agagtacaag actggtggac
    32341 atgtcattaa tttaggggat gtaggtaaga acttcggtca aggtatgagt ggtggtattg
    32401 cttacgttat cccgtctgat gtagaagctt ttgttgaaaa taatcaacta gatacacttt
    32461 cgtttacaaa gattaaacac caagaagaaa aagcattcat taagcaaatg ctggaagaac
    32521 atgtgtcaca cacgaatagt acgagagcga ttcatgtgtt aaaacatttt gatcgcattg
    32581 aagatgtcgt cgttaaagtt attcctaaag attatcaatt aatgatgcaa aaaattcatt
    32641 tgcacaaatc attacatgac aatgaagatg aagcgatgtt agctgcattt tacgatgaca
    32701 gtaaaacaat cgacgctaaa cataaaccag ccgttgtgta ttaaggaaag ggggagatac
    32761 gatgggtgaa tttaaaggat ttatgaagta tgacaaacag tacttaggtg aattatcact
    32821 ggtagaccgt ttgaagcatc ataaagcata tcaacaacga tttactaaag aagatgcctc
    32881 tatccaaggt gcgcgatgta tggattgtgg aacgccgttt tgtcaaaccg gacaacagta
    32941 tggtagggaa acaataggtt gtccaattgg aaactacatt cctgaatgga acgacttagt
    33001 gtatcatcaa gattttaaaa ctgcttatga acgcttaagc gaaacaaata actttcctga
    33061 ctttacaggg cgtgtatgtc ctgcaccatg cgaaagtgct tgtgtgatga agattaatag
    33121 agaatcgatt gcgattaaag gtattgaacg cacaattatt gatgaagctt ttgaaaatgg
    33181 ttgggtagca ccgaaagttc cgagtcgtcg tagagatgaa aaagtggcaa tcgttggaag
    33241 cggtccagca ggattaactg ctgctgaaga acttaatcta ctaggatatc aagtaactat
    33301 ttatgaacgt gctagagaat caggcggttt attaatgtat ggtattccta atatgaagct
    33361 tgataaagat gtggttcgac gtcgtattaa gttaatggaa gaagcgggca ttactttcat
    33421 taatggtgtt gaagtaggcg ttgatattga taaagcaacg ttagaatctg agtatgatgc
    33481 cattatatta tgtactggtg cacaaaaagg tagagattta cctttagaag gacgcatggg
    33541 tgatggtata catttcgcta tggattattt aactgaacaa acgcagctgt taaatggaga
    33601 aattgatgat ataacaataa ctgcaaaaaa taagaatgtc atcattattg gtgctggtga
    33661 tacaggagca gactgtgtag caacggcatt aagagaaaac tgtaaatcga ttgttcaatt
    33721 caataagtat acgaaattac ctgaagctat tacatttgaa gaaaatgctt cttggccatt
    33781 agcgatgcca gtatttaaaa tggattatgc acaccaagag tacgaagcta agtttggtaa
    33841 ggaaccacgt gcatatggtg ttcaaacaat gcgttacgat gttgacgata aaggacacat
    33901 acgtggtttg tatactcaaa ttttagagca aggcgaaaat ggtatggtca tgaaagaagg
    33961 acctgaaaga ttttggcctg ctgaccttgt attattatca atcggcttcg aaggtacaga
    34021 accaactgta ccgaatgctt ttaacattaa aacggataga aatcgaatcg tagcggatga
    34081 tacaaactat caaactaata atgaaaaagt atttgctgct ggagatgcta gacgtggtca
    34141 aagtttagtt gtatgggcga ttaaagaagg tagaggcgta gcgaaagcag tagatcagta
    34201 tttagcgagt aaagtttgtg tataatcttt gtatggaaat ggtggttacg ttgacgttgt
    34261 ggcatgctga atcgagtttg aaataatcta gtatctatca acgtcacatg ctatctttgt
    34321 aacctaaaaa caaaggtttg taagacaaca aatagattaa ttataagtag tgatttttta
    34381 cattcgttta taggtcaact gtagtggaag acaatgattt gtggtaatca tgtaatgctt
    34441 aaaaacaata ttgactttta cagaacgttc atatatgata aatattgtgt ttaggaggaa
    34501 tacccaagtc cggctgaagg gatcggtctt gaaaaccgac aggggcttaa cggctcgcgg
    34561 gggttcgaat ccctcttcct ccgccatcaa tatttatatt aaattctata tataatgaag
    34621 gtaagtgctc aaattttgag tatttacctt ttttatttgt ctttgaatgg ctcgtaattt
    34681 ttgataatag aaatgataag gcattgagat tggaagggca tttggcttgt gcaatataca
    34741 tagctaaatg tcttttttgt tttgtgaaat atgatggatg gcttgtgtgg acaagtttgc
    34801 tatttataga tatgcatttt tcaatttagg agttggccat gcatctacac tttataatgg
    34861 tgagagcgtg gtgaggtatt gttaataacg caattgtagc gaggagttat tgctacatat
    34921 gtcgttatgg ctcattgatt ttctgaaatg gctaccccag ataattgtga caaaataaaa
    34981 atatttcttt gaaagccttt acacaacttg tctagacaag ttatactcat tttaagacat
    35041 taagggagtg aaatatatgg ctgtaaaaag agaagatgta aaagccatcg taaccgctat
    35101 tgggggaaaa gaaaatcttg aagctgcaac gcattgtgta acacgattac gtttagtgct
    35161 gaaggatgaa agtaaagttg ataaagacgc attaagtaat aacgcgttgg tcaaggggca
    35221 gtttaaagca gaccatcaat atcaaattgt cattggtcca ggaacagtcg atgaagtgta
    35281 taagcagttt attgatgaaa caggtgctca agaagcttcg aaagatgaag cgaaacaagc
    35341 agctgcgcaa aaagggaatc cagtacaacg tttgatcaaa ttattggggg atatttttat
    35401 accaatatta cctgcgattg tgacagctgg tttgttaatg ggaatcaata atttactaac
    35461 aatgaaaggt ttatttggtc cgaaagcact tattgagatg tatccacaaa ttgctgatat
    35521 ttcaaacatc attaatgtga ttgcgagtac ggcatttatt ttcttaccag cattaattgg
    35581 ttggagtagt atgcgtgtat ttggtggtag tccgattcta ggcatagtct taggtttgat
    35641 tttaatgcat ccgcaattag tatctcagta tgatttggca aaagggaata ttccgacatg
    35701 gaacttattt ggcttagaga ttaagcagtt gaattaccaa ggtccagtgt tgccagtttt
    35761 aattgcagct tatgttctag ctaaaattga aaaaggatta aataaagtcg ttcacgattc
    35821 gataaaaatg ttggtcgttg gccccgtagc gcttttagtt actggatttt tagcatttat
    35881 tatcattgga ccagttgcat tattgattgg tacaggaatt acatctggtg ttacatttat
    35941 attccaacat gcaggatggc ttggcggagc aatatatgga ttgttatatg caccacttgt
    36001 aattacagga ctacaccata tgtttttagc agtagatttc caattgatgg gtagcagctt
    36061 aggtggtacg tatttatggc caatcgttgc gatttccaat atttgtcagg gctctgcagc
    36121 atttggagca tggtttgtct ataaacgtcg taaaatggtt aaagaagaag gcttggcatt
    36181 aacatcttgt atttctggta tgttaggtgt tactgaacca gctatgtttg gtgtgaactt
    36241 acctctgaaa tatccattta tcgctgcgat atcaacatct tgtgtattgg gggcaatcgt
    36301 tggtatgaat aacgtacttg gaaaagttgg tgttggtggc gtgccagcat tcatttcaat
    36361 tcaaaaagaa ttttggccag tatatcttat tgtgacagct attgctattg ttgtaccatg
    36421 tatactaaca attgtgatgt ctcattttag taaacaaaaa gcgaaagaaa ttgttgaaga
    36481 ttaataaaat aaaaaagggg cgttcgttat ttggacgtcc tttattacgt tataaggtgg
    36541 taattgtgtg tcgaaagaaa tagattggag aaaatccgtt gtatatcaaa tttatcctaa
    36601 gtcgtttaat gatacgacgg ggaatggtat aggagatatc aatggaatta tagaaaaatt
    36661 ggattatatc aagttattgg gtgttgatta tatttggtta acaccagtgt atgaatcacc
    36721 tatgaatgat aatggctatg atatcagcaa ttatttagaa atcaatgaag actttggaac
    36781 gatggatgat tttgaaaagt taatcaaagt tgcgcatcaa aaagacttga aagtgatgtt
    36841 agatattgtc attaatcata cgtcgacgga gcatgaatgg tttaaagaag cccgtaaatc
    36901 taaagataac ccttatagag attattactt tttcagatca tctgaagacg ggccgccaac
    36961 aaattggcat tctaaattcg gtggtaatgc atggaagtat gattctgaga cagatgaata
    37021 ttatttacat ttatttgatg tcagtcaagc tgatttaaat tgggataatc cggaagtacg
    37081 tcaatcgtta tatcgcatag tcaatcattg gatagacttc ggcgttgatg gttttcgatt
    37141 tgatgtcatt aacttaattt ctaaaggtga atttaaggac tctgacaaaa taggtaaaga
    37201 attttatacg gatggtccta gagtgcatga gtttctgcat gaattaaatc gtcaaacgtt
    37261 tggtaacact gacatgatga ctgtaggaga aatgtcttcg acgacgattg aaaattgtat
    37321 taagtataca cagccagaac gccaagaatt gaatagtgtt tttaattttc atcatctaaa
    37381 ggttgattat gttgatggtg aaaagtggac aaatgcgaag cttgatttcc ataagttaaa
    37441 gaaaattctg atgcaatggc aacgaggtat ttatgacggt ggcggatgga acgcgatttt
    37501 ctggtgtaat catgatcagc cacgggtagt gtctagattt ggtgatgata cgtcggaaga
    37561 gatgaggata caaagtgcta aaatgttagc tatcgcactg catatgttgc aagggacgcc
    37621 atatatttac caaggtgaag aaattggtat gacggaccca cattttacat caatagcaca
    37681 atatcgcgat gttgaatcga ttaatgccta ccatcagttg ttaagtgaag ggcatgctga
    37741 agcggatgtg ttaacgattt taggacagaa gtcacgagac aattcgagaa cgcctatgca
    37801 atggagtgat gatgttaatg ctggatttac agctggtaag ccttggattg atatttcgga
    37861 aaattatcat caggtcaacg ttagacaagc acttcagaat aaagagtcta ttttctatac
    37921 gtatcaaaaa ttaatacaat taagacatac gcatgatatt attacgtatg gagacattgt
    37981 gccacgtttt atggatcatg atcatttatt tgtttatgaa cgtcattata agaatcaaca
    38041 atggctagta attgcgaatt tctcagcatc ggctgttgat ttgccagaag gattggcgtg
    38101 cgaaggtcgt gttgtgattc aaacaggcac agtggaaaat aatacgataa gcgggtttgg
    38161 tgcaattgta atcgaaacaa acgcgtaaaa taaattgagt ggatgcgttt atatggcgaa
    38221 acaaaaaaag tttatgaaga tttatgaggc gttgaaagaa gatatattaa acgggcagat
    38281 tcaatatggt gaacaaattc cgtctgaaca tgatttggtg caattgtacc agtcatctcg
    38341 agagaccgtg cgtaaggcat tagatttgtt ggcattagac ggcatgattc aaaagattca
    38401 tggtaaaggg tcacttgtca tttatcagga ggttacagag tttccatttt ctgaacttgt
    38461 tagttttaaa gaaatgcaag aagaaatggg cgtcgtatat ttaactgaag ttgttgtgaa
    38521 tgaggttgtt gaagcgcatg aagttccaga agttcaacat gctttaaaca tcaattctag
    38581 tgaatcgctc attcatattg ttagaactcg tcggcttaac caacatgtga agattgttga
    38641 tgaagattat tttctaaagt cgattgtttc agatataggt aatgatgttg cgagtgattc
    38701 tatttatgat tatttggaaa aggtattaaa tcttaatatt agttattcaa gtaagtctat
    38761 tacttttgaa ccgtttgatg aacaagcata tcaattgttt ggtgatgtat cggtggctta
    38821 ttcagcaaca gttcgaagta ttgtgtattt agaaaataca atgccgtttc aatataatat
    38881 ttcaaaacat cttgcaaatg aatttaaatt taatgacttc tcaagacgtc gtacaaagta
    38941 aacaatgata taaatgattt atacttgcaa ttaactatta aaatatagta atatatatct
    39001 tgccgtgcta ggtggggagg tagcggttcc ctgtactcga aatccgcttt atgcgaggct
    39061 taattccttt gttgaggccg tatttttgcg aagtctgccc aaagcacgta gtgtttgaag
    39121 atttcggtcc tatgcaatat gaacccatga accatgtcag gtcctgacgg aagcagcatt
    39181 aagtggatca tcatatgtgc cgtagggtag ccgagattta gctaacgact ttggttacgt
    39241 tcgtgaatta cgttcgatgc ttaggtgcac ggttttttat tttttaaata ttaaaccgat
    39301 tattaagagt tgaaaatata tatttataga agctactttc ttgaagacaa ttcagcgtat
    39361 tatacgtgga acatgtttgt gggaagtagc ttttttatat gtgaagtttg attcaagtga
    39421 actcgatgcg cagtttgaat gatttttgtg tcaatgaaaa gtaagaagtt attatttgat
    39481 gataaagaaa tgatggtgaa atgaggggga gtatcttaca atagaattat taatgagata
    39541 cgttatgatt attgacaatc aaatgcctac ggaggacata tgcaaatata tttaagtact
    39601 ttaacagagt tagattatga taaatcttta aatagtattg aagaaagttt tgatgataat
    39661 cctgaaacga gttggcaagc acgtgcgaaa gtaaaacatt taagaaaatc tccttgctat
    39721 aattttgaat tagaagtaat agcgaaaaat gaaaataacg atgtcgttgg acacgtttta
    39781 ttaattgaag tagaaattaa tagtgatgat aagacgtatt atggtttggc gattgcctct
    39841 ttatcagttc atcctgaatt acgtggacaa aaattaggtc gtggcttggt tcaagcagta
    39901 gaagagcgtg ccaaagcaca agagtatagt acggttgttg tagaccattg ttttgactac
    39961 tttgaaaagt tgggttatca aaatgctgct gagcatgaca ttaaattaga atctggtgat
    40021 gcaccgttac ttgtaaaata tttatgggat aatttgacgg atgcaccaca cggaatcgta
    40081 aaatttccag aacattttta ttaattgttc aattaagaag taaaggtatt atcatgctat
    40141 aatgagaggt aattgtttat ggaggtgcta acttgaatta tcaagcctta tatcgtatgt
    40201 acagacccca aagtttcgag gatgtcgtcg gacaagaaca tgtcacgaag acattgcgca
    40261 atgcgatttc gaaagaaaaa cagtcgcatg cttatatttt tagtggtccg agaggtacgg
    40321 ggaaaacgag tattgccaaa gtgtttgcta aagcaatcaa ctgtctaaat agcactgatg
    40381 gagaaccttg taatgaatgt catatttgta aaggcattac gcaggggact aattcagatg
    40441 tgatagaaat tgatgctgct agtaataatg gcgttgatga aataagaaat attagagaca
    40501 aagttaaata tgcaccaagt gaatcgaaat ataaagttta tattatagat gaggtgcaca
    40561 tgctaacaac aggtgctttt aatgcccttt taaagacgtt agaagaacct ccagcacacg
    40621 ctatttttat attggcaacg acagaaccac ataaaatccc tccaacaatc atttctaggg
    40681 cacaacgttt tgattttaaa gcaattagcc tagatcaaat tgttgaacgt ttaaaatttg
    40741 tagcagatgc acaacaaatt gaatgtgaag atgaagcctt ggcatttatc gctaaagcgt
    40801 ctgaaggggg tatgcgtgat gcattaagta ttatggatca ggctattgca tttggtgatg
    40861 gtacgttaac attgcaagat gcgttgaatg tcacaggtag cgtacatgat gaagcgttgg
    40921 atcacttgtt tgatgatatt gtacaaggtg acgtacaagc atcttttaaa aaataccatc
    40981 agtttataac agagggtaaa gaagtgaatc gcctaataaa tgatatgatt tattttgtca
    41041 gagatacgat tatgaataaa acatctgaga aagatactga gtatcgagca ctgatgaact
    41101 tagaattaga tatgttatat caaatgattg atcttattaa tgatacatta gtgtcgattc
    41161 gttttagtgt gaatcaaaac gttcattttg aagtgttgtt agtaaaatta gctgagcaga
    41221 ttaagggtca accacaagtg attgcgaatg tagctgaacc agcacaaatt gcttcatcgc
    41281 caaacacaga tgtattgttg caacgtatgg aacagttaga gcaagaacta aaaacactaa
    41341 aagcacaagg agtgagtgtc gctcctactc aaaaatcttc gaaaaagcct gtgagaggta
    41401 tacaaaaatc taaaaatgca ttttcaatgc aacaaattgc aaaagtgcta gataaagcga
    41461 ataaggcaga tatcaaattg ttgaaagatc attggcaaga agtgattgat catgccaaaa
    41521 acaatgataa aaaatcactc gttagtttat tgcaaaattc ggaacctgtg gcggcaagtg
    41581 aagatcacgt acttgtgaaa tttgaggaag agatccattg tgaaatcgtc aataaagacg
    41641 acgagaaacg tagtagcata gaaagtgttg tatgtaatat cgttaataaa aacgttaaag
    41701 ttgttggtgt accatcagat caatggcaaa gagttcgaac ggagtattta caaaatcgta
    41761 aaaacgaagg tgatgatatg ccaaagcaac aagcgcaaca aacagatatt gctcaaaaag
    41821 caaaagatct tttcggtgaa gaaactgtac atgtgataga tgaaaagtga tacatgacaa
    41881 gcgatataat cgtatgtata atgaaagaaa catcatttta ttgataaata tttattgatt
    41941 ttcaaggagg aaatggaata tgcgcggtgg cggaaacatg caacaaatga tgaaacaaat
    42001 gcaaaaaatg caaaagaaaa tggctcaaga acaagaaaaa cttaaagaag agcgtattgt
    42061 aggaacagct ggcggtggca tggttgcagt tactgtaact ggtcataaag aagttgtcga
    42121 cgttgaaatc aaagaagaag ctgtagaccc agatgatatt gaaatgctac aagacttagt
    42181 gttagcagct actaatgaag cgatgaataa agctgatgag cttactcaag aacgtttagg
    42241 taaacatact caaggcttaa acatccctgg aatgtgatca tagatgcatt atccagaacc
    42301 tatatcaaaa cttattgata gctttatgaa attgccaggc attggtccaa agacagccca
    42361 acgtctgact tttcatacct tagatatgaa agaagacgat gttgttcagt ttgccaaagc
    42421 attagtagat gttaagagag aattaacata ttgtagcgta tgtggtcaca ttactgaaaa
    42481 tgatccatgt tatatttgtg aagataagca aagagatcgt tcagttattt gtgttgtgga
    42541 agatgacaaa gatgtcatag ctatggaaaa aatgagagaa tacaaaggtt tatatcacgt
    42601 tttacatggg tctatttcgc ctatggatgg cattggacca gaagatatta atattccttc
    42661 attgattgaa cgcttgaaaa acgatgaagt tagcgaatta atcttagcta tgaacccgaa
    42721 cttagagggg gaatctacag ccatgtatat ttctagatta gttaagccta taggtatcaa
    42781 agtgacgaga ttagcacaag ggttatcggt aggtggcgat ttagagtatg ctgacgaagt
    42841 aacattatct aaagcaatcg caggtagaac agaaatgtaa tgtcttctat taaacatttt
    42901 tgattttaat actatagtaa gaaaagtcac agtgtaatca ttgtggcttt ttttatggtg
    42961 tggtgtgatg tactacttta tttgcggtgt ggcggtggca tggtttacct agttttaatg
    43021 gggggggtca tcttaagaaa gaaagtcgtt agttgagatt tgttccttct aatagtaatg
    43081 atgtgaattg gattatcgaa ttagatttac ggttatggtg tgttggtgct attaatttga
    43141 taaatgcggt taatgactat gcaaatgaaa ttcttttgta attgaaatga tagatgctgg
    43201 cttagtagtt gtacttcttt ggtctaaagc ttattaaatc agcctgtata gcggtgtttt
    43261 gagagattat ttaaaacttg taaatttatt tttaatttct ggtaaaaaaa taacgttctg
    43321 ttttgcggtt tttttgattg atatggttag agaaaaatct gtttcttgtt ctaaaaaacg
    43381 tactatttat aagtggggat tttttaagtt cgatttttag gataagggcg ttcagtacag
    43441 atgacaaagg tgtaattttt actgttgtta agcagtttga aagcctgtat agtatttatt
    43501 tgttgaggca aacaaaacaa ttcaacttaa gaaataactt gaattactaa cgaaaattaa
    43561 ttttaaaaag ttgttgactt aaatgttaat aaaatgtata attaattctt gtcggtaaga
    43621 aaaaatgaac attgaaaact gaatgacaat atgtcaacgt taattcc
//
LOCUS       DAHHYJ010000005        42704 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000005 DAHHYJ010000000
VERSION     DAHHYJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 42704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42704
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.005"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            83..1249
                     /locus_tag="NBK22_000214"
     CDS             83..1249
                     /locus_tag="NBK22_000214"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCD2413432.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(1414..1935)
                     /locus_tag="NBK22_000215"
     CDS             complement(1414..1935)
                     /locus_tag="NBK22_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCD2413433.1"
                     /translation="MEYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGTREENTSLA
                     IFREATQWLDAYFKGDNPEITIPLKPTGSHFQQCVWNELRQVPYGTLTTYGAIAKKVG
                     KLLNKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            2344..4449
                     /gene="clpL"
                     /locus_tag="NBK22_000216"
     CDS             2344..4449
                     /gene="clpL"
                     /locus_tag="NBK22_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCD2413434.1"
                     /translation="MNNGFFNSDFDSIFRRMMQDMQGSNQVGNKKYYINGKEVSPEEL
                     AQLTQQGGNHSAEQSAQAFQQAAQRQQGQQGGNGNYLEQIGRNLTQEARDGLLDPVIG
                     RDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEIISV
                     DISSLEAGTQYRGAFEENIQKLIEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSKGLS
                     DILKPALSHGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILKGIR
                     EKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAVDKVE
                     TEKRISELENDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDISDTI
                     QRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGNRPI
                     GSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGYVGY
                     DDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFKNTI
                     IICTSNAGFGNGNDAEEKDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVNLLL
                     DDVQVTLDKKGITMDVSQDAKDWLIEEGYDEELGARPLRRIVEQQVRDKITDYYLDHT
                     DVKHVDIDVEDNELVVKGK"
     gene            complement(4511..4681)
                     /locus_tag="NBK22_000217"
     CDS             complement(4511..4681)
                     /locus_tag="NBK22_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCD2413435.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDINRDCCG
                     TEQHTANHFPGK"
     gene            complement(4694..6688)
                     /gene="feoB"
                     /locus_tag="NBK22_000218"
     CDS             complement(4694..6688)
                     /gene="feoB"
                     /locus_tag="NBK22_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCD2413436.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVDKQVQAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTILKNDNAIFIVELPTYRVPSIKPLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVEPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDASLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(6700..6927)
                     /locus_tag="NBK22_000219"
     CDS             complement(6700..6927)
                     /locus_tag="NBK22_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCD2413437.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(7143..9632)
                     /gene="farE"
                     /locus_tag="NBK22_000220"
     CDS             complement(7143..9632)
                     /gene="farE"
                     /locus_tag="NBK22_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCD2413438.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAG
                     SAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVIFAVFSALTLLPALISIFHKRI
                     KVNKLQSNFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKQQATNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            9777..10325
                     /gene="farR"
                     /locus_tag="NBK22_000221"
     CDS             9777..10325
                     /gene="farR"
                     /locus_tag="NBK22_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCD2413439.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTMSDTINNKEDLREIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSDIPDNLIFYIYDSLIEGFIHWIKDE
                     KIDWPGEDIDNIFHRLINIKIK"
     gene            complement(10402..10569)
                     /locus_tag="NBK22_000222"
     CDS             complement(10402..10569)
                     /locus_tag="NBK22_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413440.1"
                     /translation="MRYVISIIMGIVLAIWSFKQLSQSHLDSGFIFFFIVYVLCISYF
                     NSDKHDKNKKR"
     gene            complement(10681..12225)
                     /gene="pruA"
                     /locus_tag="NBK22_000223"
     CDS             complement(10681..12225)
                     /gene="pruA"
                     /locus_tag="NBK22_000223"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCD2413441.1"
                     /translation="MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(12415..13014)
                     /locus_tag="NBK22_000224"
     CDS             complement(12415..13014)
                     /locus_tag="NBK22_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCD2413442.1"
                     /translation="MTEKEKMLAEKWYDANFDQYLINERARAKDICFELNHTRPSATN
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            13253..13444
                     /gene="cwrA"
                     /locus_tag="NBK22_000225"
     CDS             13253..13444
                     /gene="cwrA"
                     /locus_tag="NBK22_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCD2413443.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQLSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            13683..16091
                     /locus_tag="NBK22_000226"
     CDS             13683..16091
                     /locus_tag="NBK22_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCD2413444.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAVLSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHQIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQITKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            16244..16450
                     /gene="copZ"
                     /locus_tag="NBK22_000227"
     CDS             16244..16450
                     /gene="copZ"
                     /locus_tag="NBK22_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCD2413445.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(16540..17538)
                     /locus_tag="NBK22_000228"
     CDS             complement(16540..17538)
                     /locus_tag="NBK22_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD2413446.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLDALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVKNLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(17561..18715)
                     /locus_tag="NBK22_000229"
     CDS             complement(17561..18715)
                     /locus_tag="NBK22_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD2413447.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQKALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKGTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNVFKTRRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVQEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(19143..20651)
                     /locus_tag="NBK22_000230"
     CDS             complement(19143..20651)
                     /locus_tag="NBK22_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2413448.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTACGKNYEDYIELRQLRYIYDVYFDHDDRITVPTDL
                     AELQQMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDSKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLATIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(20663..21526)
                     /locus_tag="NBK22_000231"
     CDS             complement(20663..21526)
                     /locus_tag="NBK22_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCD2413449.1"
                     /translation="MTMMDMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQHFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFDNERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFQDVMDQIKVFSIEAQPIIELAVRIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(21563..22690)
                     /gene="crtQ"
                     /locus_tag="NBK22_000232"
     CDS             complement(21563..22690)
                     /gene="crtQ"
                     /locus_tag="NBK22_000232"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCD2413450.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKALTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDTDGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQGEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGAGGTKPK
                     IMTAIVLWLFGSIASILGLCLSLKYRQMSVRKMVALYLSYTTQFIYLHRRIGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(22696..24189)
                     /locus_tag="NBK22_000233"
     CDS             complement(22696..24189)
                     /locus_tag="NBK22_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2413451.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKLFEYSKKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNAILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLLHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDSTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVEADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(24182..24670)
                     /gene="crtO"
                     /locus_tag="NBK22_000234"
     CDS             complement(24182..24670)
                     /gene="crtO"
                     /locus_tag="NBK22_000234"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCD2413452.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKYIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRAEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMIANVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(24845..25612)
                     /locus_tag="NBK22_000235"
     CDS             complement(24845..25612)
                     /locus_tag="NBK22_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD2413453.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWANAAASSGYTVNNTPKVGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(25973..27784)
                     /locus_tag="NBK22_000236"
     CDS             complement(25973..27784)
                     /locus_tag="NBK22_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD2413454.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYIIEILLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
     gene            complement(28470..29171)
                     /gene="isaA"
                     /locus_tag="NBK22_000237"
     CDS             complement(28470..29171)
                     /gene="isaA"
                     /locus_tag="NBK22_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCD2413455.1"
                     /translation="MKKTIMASSLAVALGVTGYAASTGHEAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLSAWGF"
     gene            complement(29778..30833)
                     /locus_tag="NBK22_000238"
     CDS             complement(29778..30833)
                     /locus_tag="NBK22_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCD2413456.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVILIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(30960..31088)
                     /locus_tag="NBK22_000239"
     CDS             complement(30960..31088)
                     /locus_tag="NBK22_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413457.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYR"
     gene            31243..31812
                     /locus_tag="NBK22_000240"
     CDS             31243..31812
                     /locus_tag="NBK22_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2413458.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDCNLPFLIHHIAHF"
     gene            32010..32510
                     /locus_tag="NBK22_000241"
     CDS             32010..32510
                     /locus_tag="NBK22_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413459.1"
                     /translation="MTSIFVFAYLNNTSGNMLFRQQMICACLLANVRHSSIVCLNNIC
                     CIVDMRCLNTKIGGIDVSETDEPQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIARGKKRIDYRLSYNFYTTYYDNVEINKIDCEYTYAMPIAILIRHTMSFDNAHFFAQ
                     LRHPVL"
     gene            32730..32960
                     /locus_tag="NBK22_000242"
     CDS             32730..32960
                     /locus_tag="NBK22_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCD2413460.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(33110..33487)
                     /locus_tag="NBK22_000243"
     CDS             complement(33110..33487)
                     /locus_tag="NBK22_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2413461.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGIVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(33504..34325)
                     /locus_tag="NBK22_000244"
     CDS             complement(33504..34325)
                     /locus_tag="NBK22_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCD2413462.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDNVSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSGVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQNYNK"
     gene            complement(34345..34644)
                     /locus_tag="NBK22_000245"
     CDS             complement(34345..34644)
                     /locus_tag="NBK22_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCD2413463.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKVL
                     AMTAPLGIFSHQLQRFIHLVWDVRDVINDNIKGNGQTPEPYTYLKGEKEDYWFLR"
     gene            34775..35332
                     /locus_tag="NBK22_000246"
     CDS             34775..35332
                     /locus_tag="NBK22_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2413464.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSNYEVMKVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFDFYYDLFKSDDDIYKKFKTDMLLQALSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVERND"
     gene            35325..36029
                     /locus_tag="NBK22_000247"
     CDS             35325..36029
                     /locus_tag="NBK22_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD2413465.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVDDFSNNSKHVSEGIKPIIQLATIGA
                     DGPTGTFINGDGEMPW"
     gene            36127..37138
                     /locus_tag="NBK22_000248"
                     /pseudo
     CDS             36127..37138
                     /locus_tag="NBK22_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
     gene            37158..37988
                     /locus_tag="NBK22_000249"
     CDS             37158..37988
                     /locus_tag="NBK22_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2413466.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKY
                     SDKITLLNGTDSRGSFPQDVNFYINKETSIPIVDIPGGHLGYIQKPEGFADVLLNMWG
                     "
     gene            complement(38135..39025)
                     /locus_tag="NBK22_000250"
     CDS             complement(38135..39025)
                     /locus_tag="NBK22_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD2413467.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVSQMTATSENKHESLHKTINHQFVQ
                     SPAKCTKAQFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
     gene            complement(39122..40462)
                     /locus_tag="NBK22_000251"
     CDS             complement(39122..40462)
                     /locus_tag="NBK22_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCD2413468.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII"
     gene            complement(40456..41562)
                     /locus_tag="NBK22_000252"
     CDS             complement(40456..41562)
                     /locus_tag="NBK22_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCD2413469.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYRIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVHTPHEPILATGFDATKNPI
                     VQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC"
     gene            complement(41857..42153)
                     /locus_tag="NBK22_000253"
     CDS             complement(41857..42153)
                     /locus_tag="NBK22_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCD2413470.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPILEIENQKYMFDYSACKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFENMKQNLDSEIQRGEVTPQ"
     gene            complement(42272..>42704)
                     /locus_tag="NBK22_000254"
     CDS             complement(42272..>42704)
                     /locus_tag="NBK22_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD2413471.1"
                     /translation="NGNQNNKSMVLGGIGVTIGIFLKGFVDAVNISISTTLLLILFII
                     AMLASILLKINLSKKLKINIDNHHYFGSVILIPTVKNFLLVICIYIVNIMLVIGLFVG
                     NIFYNKQNVLTYIIWILMFFIFMCLNMTSISDKKIHIKIKN"
BASE COUNT        13953 a         7912 c         6618 g        14221 t
ORIGIN      
        1 agtatatttc tttgtataaa attagcatat tctgatatga tacaagtgtt gcttttataa
       61 atttgaaagg atgtaaaacc ttatgacaat aggtatcgat aaaataaact tttacgttcc
      121 aaagtactat gtagacatgg ctaaattagc agaagcacgc caagtagatc caaacaaatt
      181 tttaattggt attggtcaaa ctgaaatggc tgttagtcct gtaaaccaag acatcgtttc
      241 aatgggcgct aatgctgcta aggacattat aacagacgaa gacaaaaaga aaattggtat
      301 ggtaattgtg gcaactgaat cagcagttga tgctgctaaa gcagccgctg ttcaaattca
      361 caacttatta ggtattcaac cttttgcacg ttgctttgaa atgaaagaag cttgttatgc
      421 tgcaacacca gcaattcaat tagctaaaga ttatttagca actagaccga atgaaaaagt
      481 attagttatt gctacagata cagcacgtta tggattgaat tcaggcggcg agccaacaca
      541 aggtgctggc gcagttgcga tggttattgc acataatcca agcattttgg cattaaatga
      601 agatgctgtt gcttacactg aagacgttta tgatttctgg cgtccaactg gacataaata
      661 tccattagtt gatggtgcat tatctaaaga tgcttatatc cgctcattcc aacaaagctg
      721 gaatgaatac gcaaaacgtc aaggtaagtc gctagctgac ttcgcatctc tatgcttcca
      781 tgttccattt acaaaaatgg gtaaaaaggc attagagtca atcattgata acgctgatga
      841 aacaactcaa gagcgtttac gttcaggtta cgaagacgct gtagattata accgttatgt
      901 cggtaatatt tatactggat cattatattt aagcctaata tcattacttg aaaatcgtga
      961 tttacaagct ggtgaaacaa tcggtttatt cagttatggc tcaggttcag ttggtgaatt
     1021 ttatagtgcg acattagttg aaggctacaa agatcattta gatcaagctg cacataaagc
     1081 attattaaat aaccgtactg aagtatctgt tgatgcatat gaaacattct tcaaacgttt
     1141 tgatgacgtt gaatttgacg aagaacaaga tgctgttcat gaagatcgtc atattttcta
     1201 cttatcaaat attgaaaata acgttcgcga atatcacaga ccagagtaat ctgtgtattt
     1261 gaaagacatt taaatataac ctaaaaacag cagtaagaca gcttctcatt gaaatcgtct
     1321 tactgctgtt ctctatttat aacacttcgt attgaatgaa ttcattatgc ctatttgaca
     1381 cattattgaa atttttaata taccccttat acgttacggc ttcgtgctat gttttggtac
     1441 ataaagcttt gacatatcga tattctccaa ctctaacaac ttaattttat tattaatcgt
     1501 tccaccgaac cctgttaagc tacccgtttt accgacaaca cgatgacatg gcacgataat
     1561 agataatgga ttacttccga ctgcacctcc aaccgcttgg gctgacattt ttggcttgtt
     1621 aagcagcttg cctacttttt tggcaatagc accatacgtt gttagagtcc cataaggaac
     1681 ctgtcttaat tcattccaaa cacactgttg aaaatgacta cctgttggct ttaaaggtat
     1741 tgtgatttca ggattgtcac ctttaaaata cgcgtctaac cactgtgtcg cctctctaaa
     1801 tatcgctaaa gacgtatttt cttccctagt accatcacct tgttgatttt caaacaaaac
     1861 agcggtcaga cttaccccat cactcaaaag ttccaatcgt cctacaggcg aatcatagta
     1921 actcttatac tccataaaaa ttcccccttt ttctaatcat acgccttaat tgttaaatta
     1981 gaaaatattc atgttcacat tttttaaaaa taaataagtc gtcaattaca tatataaaca
     2041 cgtaatacca gctatcactt tgctgcaata tacagttaca tatcttacta cacgtgctaa
     2101 cctcttactt tgtaaaccaa atcttaaatt aaaatattga aaatgcaatg gatccttaat
     2161 attttattaa acctataatt acttattaaa aataacacac aatattcata aagttttaaa
     2221 aatattctat tttatcacct attattagtg gaaaagtaca attgcaattg tatatagttt
     2281 gcataacgct tcaaaagtaa tttctttttt gtttagttca aaaaaattta gaggtgatgt
     2341 tatatgaata acggtttttt caatagcgac tttgattcaa tttttcgaag aatgatgcaa
     2401 gatatgcaag gttcaaatca agtcggtaac aaaaagtact atattaatgg taaagaagtt
     2461 tcacctgaag aactagcgca actcacacaa caaggtggca atcactctgc tgaacaaagt
     2521 gcgcaagctt ttcaacaagc agcacaaaga caacaagggc aacaaggtgg caacggcaat
     2581 tatttagaac aaattggtcg taaccttacg caagaagcac gtgacggttt attagatcca
     2641 gtcattggtc gtgataaaga aattcaagaa actgctgaag tcttaagtag acgaactaaa
     2701 aacaatccta tattagttgg agaagctggt gttggtaaaa ctgcgattgt tgaaggttta
     2761 gcacaggcaa tcgttgaagg aaatgtacca gcagcaatca aagacaaaga aattatttct
     2821 gtagatattt catcattaga agctggaacg caatatcgtg gtgcttttga agaaaatatt
     2881 caaaaattaa tcgaaggtgt taaatcttca caaaatgccg tactattctt tgatgaaatc
     2941 catcaaatta tcggttcagg tgccacagga agtgattcag gtagcaaagg gttatctgat
     3001 attttgaaac ctgcattaag tcatggtgag atttctatta ttggtgcaac aacacaagat
     3061 gaatatcgaa acaatattct taaagatgct gcattaacgc gcagatttaa tgaagtactt
     3121 gttaatgaac caagtgctaa agatactgtg gaaattttaa aaggtattcg cgaaaaattc
     3181 gaagaacacc atcaagtaaa attaccagat gacgtattaa aagcatgtgt tgacttatca
     3241 attcaatata ttccacaacg attattacca gataaagcaa tcgatgtgtt agatattaca
     3301 gcagcacatt tatctgcgca aagtccagct gtcgataaag ttgaaactga aaaacgaatt
     3361 tctgaattag aaaatgataa acgtaaagcg gtaagtgctg aagaatataa aaaagctgac
     3421 gacattcaaa atgaaatcaa atcattacaa gataaattag aaaatagtaa tggtgaacat
     3481 actgctgttg ctacagttca tgatatttca gatactattc aacgattaac tggtattcca
     3541 gtttctcaaa tggatgataa cgatattgaa cgtttaaaaa atatttctaa tcgtttaaga
     3601 agtaaaatca taggtcaaga tcaagctgta gaaatggttt cacgtgcaat tcgccgtaat
     3661 cgtgctgggt ttgatgacgg caaccgtcca attggcagtt tcctatttgt tggccctact
     3721 ggtgttggta aaacagagct tgctaaacaa ttagcaattg atctatttgg taataaagat
     3781 gcactgattc gacttgatat gagtgaatat agtgacacaa cagctgtttc aaaaatgatt
     3841 ggtacaactg ctggttatgt cggttatgat gacaattcaa atacgttaac tgaaaaagta
     3901 cgccgtaatc catactcagt cattctattt gatgaaatcg aaaaagcaaa tccacaaatt
     3961 ttaacattgt tattacaagt aatggatgat ggtaatttga ctgatggtca aggtaatgtc
     4021 atcaacttta aaaatacaat tattatttgt acatcaaatg ctggctttgg caatggcaat
     4081 gacgctgaag aaaaagatat tatgcacgaa atgaaaaaat tcttccgccc tgaattcctt
     4141 aaccgcttca acggcatcgt tgaattctta catttagata aagatgcatt gcaagatatc
     4201 gtcaacttat tattagacga tgtacaagtt acattagata aaaaaggtat tacgatggac
     4261 gtttctcaag atgcgaaaga ttggttaatt gaagaaggct atgatgaaga attaggtgca
     4321 cgtcccttaa gacgtattgt tgaacagcaa gtacgtgaca aaattacaga ttactattta
     4381 gatcatacag acgttaaaca tgtggatata gatgttgagg ataacgaatt agtcgtaaaa
     4441 ggtaaataac gacactttaa catatcaaaa atgagcatca ggtcgccctt gcctgtgctc
     4501 atttttttaa ttatttccct ggaaaatgat tcgctgtgtg ctgttctgtt ccacaacaat
     4561 cacgattaat gtcacatgta ccacattttc cttgtttcga acgtttgaaa aatttcacta
     4621 gtgtatacaa ggcatatccg aaaattgcta aaaaaattaa aatgttaata atgactgaca
     4681 ttttaaccac tccttaaaca aataaatgtc cgacttgata aaaaatgaat gttaagacat
     4741 atgcagtgac tagaggatag gcaactgcaa gtgccgtcca tttccatgaa taagtctctt
     4801 tacggattgc tgccactgta gaaacacaag gaatatacaa tagtataaat atcataaatg
     4861 catacgcaga tagcggtgtg aattgatttt gaatcacatt gacaaggctt gcatcacctg
     4921 atgaatagat aatcgccatc gaacttacga taacttcttt tgctaaaaat cctggcacta
     4981 acgtagcacc tgcttgccat gttccaaatc cgagcggttc cactaacata ccaaagaagc
     5041 taccaaccat atgtaaaaaa ctttgattga tattcacatt gataccatgt ggccctacat
     5101 aacttaatag ccaaatgact actgagccgc caaaaataaa tgtacctgct ttacgaacaa
     5161 agcccttagc cttttcccaa gtactacgcc acaacggttt aatggaagga acacggtatg
     5221 ttggcaattc cacaataaag attgcattat cattttttaa aatcgtctta gtaagtactg
     5281 tactgactaa aaatgccata ataataccta aaacatacag gcttaatact actaaagatt
     5341 gattctcttt gaaaaagata cctacgaaca acgcatacac tggcagtcta gcagagcatg
     5401 acatgaatgg tgcaattaat atcgttgtta aacgctcttt ttcattttca atactgcgcg
     5461 cagccataat actcggtacg ttacaaccaa atccgataat catcggtata aaagacttcc
     5521 cgcttaaacc gaacgattcc ataatacgat ccattaaaac cgcaatacgt gccatataac
     5581 ccgaatcttc taataaagat ataaagaaaa agagcacaac aatttgtggt acaaagacta
     5641 atactgatcc tacaccagca ataatgccat ctgtaattaa atcttgtaaa aatggtataa
     5701 caccaagata attcataatc gtcttcacac tatcggtaaa tgtaccacct ataaatgcat
     5761 cgagttgatc cgacaaaggt gtgccaatcc atgtaaatgt agtttgaaag atcaaccaca
     5821 taatcgctag aaaaataggc atccctatat atttatgtgt taatatcttg tctattctag
     5881 agctgaaata ttgcttatct tcatctggat acgttaccac gtcttgcaat aacgtctcaa
     5941 tataatgatt gcgtatacgc tccatctctc gacgaacaga tacagcctgt acttgtttat
     6001 caacttgatc acgtaaactc gacaatttat ttacaacctc tgaattaagt tcgtttgcaa
     6061 tttgcatgtt atttaataag aattgaatcg caataaatct agcttgatac ttatcatgag
     6121 atgtctctgt cattattatt tgacacatat ttttaattgt ctcttcaatc ttctcaccat
     6181 aattgatttt aaaatgcggt tgataccctt cccctagatg ctttatttcg ccaagtaaat
     6241 gtttagttcc tttgcctgta cgtgccacaa ctggaaaaat aggtgttttt aactttttca
     6301 tcaatttatg ataatcgatt tttatcccgc gctttgtagc tacatcaatc atatttaatc
     6361 cgatgtatat tggttgatta agttctaaca attgtactgt taattgcata tttcttttta
     6421 gttgactcgc atcaacaatg ttaatgattc ctgaaaatga atcgtttaat aaatagtctg
     6481 tcactacagt ttcatcttta gaaatcggcg ataaatcata tgtacctggt aaatcaatta
     6541 attgtcctac attttctttt agcttcccta cttttttctc taccgttacg ccactccagt
     6601 tgcctatata ttcatacgaa ccagttaaag cgttaaacaa agatgtttta ccaacattag
     6661 gatttcctaa aatacaataa ttttccattc gtccggctcc tattcttcta atgcaataga
     6721 acaagcatcg caatgcctaa tacttaactg ttgtccgttt acttcaataa tacatggccc
     6781 tttaaataaa catttttgtt taatcgttat gatagcgtca tctgttaacc caaaggcact
     6841 tagacgatac aacatattct cattagcaat atccattcgc tttattttat aagccttatt
     6901 catttcacca tttttaatgt ttaacatact atttcgctct cctattagaa ataataatca
     6961 ttatcactta aaaatcataa cccttaaaat tgtagctcgc aatactttat ttaaataatt
     7021 ttcatttttc atgtaaaatt tgtgacattg caaaaatgtt ttgtataatc acttccatgc
     7081 aaaaaacctc cgcttcaaat gtcattgata gacatttaaa gcggaggcat gtacatgtta
     7141 ttttatgatt tatttcgtag atttttttct aacaattgat tgagcttttc aatattttta
     7201 ttcaactgtt ctatattcgt tgcttgttgt tttgaattta gttgatgctt gttttgataa
     7261 tctattaaag catcatataa gaacaatgaa cttgctgatt gctgtttcac ttcttggtac
     7321 aatgccatcg ttttatcatc tagtactatt gaatgtgcat catcttgccc atgtgcatct
     7381 tgatgtttcg atacatttgt tgcaccttgt ggatatactt taaaggttgt gtcgtatgtt
     7441 tccttactat ctttagcttc agattcctgt gattcaaccg ttttatattt ttcaagtgca
     7501 tgtccttcaa tatcaactcg tggaataatg cgattcaacc atgctggtaa ataccacgaa
     7561 ccttttccaa acaatttcgt taatgcagga attaacatca ttctgactac gaaggcatca
     7621 aagagtacac caaacgctaa tgccataccc attgatttaa tcatgacatc ttcttggaat
     7681 acaaacgcaa agaagacact aaacataatt aatgcagctg ctacaataac aggaccgctt
     7741 tctttcaatc ctactttgat agaataatca ttatcccctg ttttactata ttcttcatga
     7801 attcgcgaca taaggaagac ttcataatcc atcgctaatc caaataagat acctatagta
     7861 ataaccggta aaaatgctag cattggtcct gtcgtttcaa taccaaacag acctttcata
     7921 aaaccatctt gcattactaa tgttgtaaat cctaatgttg ccattaatga caagacgaat
     7981 cctaaaactg cttttaatgg tattagaatt gaacggaaga caatcattaa taagaaaaat
     8041 gctaatacaa caatgactga ggcaaataaa ggtatcgcct catttaactt tttagacata
     8101 tcaatattaa tgacactttg tcccgaaatc tccgttttga acccatattt atcttgtgca
     8161 tctttatgat aatctcgtaa atcatgcact aaatcatttg tactctctgc attaggccct
     8221 tgcttaggta tcacgaccat caaagcgtaa tcattatctt tactcatttg tggcggcgta
     8281 acgatatcta catttttctt atctttaata tctttatata cagactgtaa atcttgttgt
     8341 aatccttgtg gatcatcctt tttatctttc acatttatca acatcggaat ttggccatta
     8401 aatccttcac caaatttatc cgagattata tcgtaagctt ttttctgtgt agaatctgct
     8461 ggtttaacac cgtcatctgg aataccaagt cgcatatgac taactggtat tgcagctgct
     8521 actaatatga ttaaacctag taatactgcc gcaagtgcat ttcctgtaat aaatttagac
     8581 caaggcgtat caatatcttt tttaaaatta gactgcaatt tgttcacttt aatgcgttta
     8641 tggaaaatac taattaacgc aggtaataaa gtcagtgcac tgaatacagc aaatataaca
     8701 ctaattgccg aagcaaaccc catcactgct aagaagtcaa tgcctactaa tgataaacca
     8761 catacggcaa ttacaactgt tacaccagca aaaacaactg cactacctgc tgttcctatt
     8821 gcaagaccaa tgcctttaat gtaatctgtt tcagttttca taacttgtcg atatctgaat
     8881 aaaataaaca aagcataatc gataccaact gctagtccaa tcattacggc taatgtcaat
     8941 gtgacatttg gtatatcaaa tgcataagtt aacaaactga taatacctac accagaggct
     9001 agaccaatca atgcacttat aattggtaat cctgcagcaa tgactgaacc aaatgtaatc
     9061 aataacacta caaatgcaac aataatacca actagttcag aattaccgcc tacttctgta
     9121 gatgtcattc ctgtaccagt taactctgtt tgtacattat gattgtcacg taagtctttc
     9181 aaatgacttt taacattatc tctagagcca tcttttaaag atgtttgact aacgtcatat
     9241 gtgatatctg caaatgctgt tgttttatct ttactaattt gcttattttc ataaggatct
     9301 gatattttat caatgtgctt gtcatctttt ttaatatcat ctaacgtttt cttaatatct
     9361 ttagtaatgt tcggttgcac aataccatca tctttagtcg tcttaaagac aacacgtatt
     9421 tgtgcctttt cactatcttg attaaaatgt ttttcaatct ttttattcgt atctaacgac
     9481 tctaatcctg tcattttaat atcattgtca aatttcggtg catttgtagc aagtggtatc
     9541 aatattgcag ctacaatcac tatccatgca atgaccgcgg accatttatg ttttgcgatg
     9601 aatgtcccca tcttatataa aaattttgcc aaagtatatt gcctcctttt aaaatcaacg
     9661 ttatagttta aatatacagt gtagattatt gttcgattat agtatctatc cccgacctct
     9721 taaagaatca attggaaaat tttgtatatt aaactacaca caaaggagaa atgtagatga
     9781 aagagactga tttacgagtt ataaagacaa aaaaagcatt gtcgagtagc ttgctacaat
     9841 tgttagaaca gcaattattc caaacgatta ctgtcaatca aatttgcgac aatgcactcg
     9901 tacaccgcac aacattttat aaacattttt atgataaata tgatcttcta gagtatttgt
     9961 tcaatcaatt gactaaagac tatttcgcca gagacattag cgatcgcctt aaccatccat
    10021 tccaaacgat gagtgatacg attaataata aagaggattt gagagaaatc gcagaattcc
    10081 aagaagaaga cgctgaattt aataaagtat taaaaaatgt ctgcattaaa attatgcata
    10141 acgatatcaa aaataataga gaccgtatcg atattgacag cgatatccca gataatctca
    10201 tattttatat ttatgactcg ttgattgaag gttttataca ttggataaaa gatgaaaaaa
    10261 ttgactggcc tggcgaagat attgataaca ttttccatag attaatcaat attaagatta
    10321 aatagtagat gagaaactca tgagcgttac caacattcat aataaaaacg atagtgtaca
    10381 cgttaatgaa ttcgtgtact actatcgttt tttattttta tcatgcttat cgctattaaa
    10441 ataactgata cacaacacat aaactatgaa gaaaaaaata aatccgctat ctaaatgact
    10501 ttgactcagt tgtttaaatg accaaattgc taatacaatt cccattatta ttgaaataac
    10561 gtatctcaca ttcttatacc tataatcctt ttctaaaaat atggttgcta ttacttaatt
    10621 tttaaagtta taaataaaaa gagccaaccg caatagatgg cccttgttca ttatgaagca
    10681 ttagaacatt tctgaaacaa ccttttgttc taagaagtgt aataagtagt ctgggctacc
    10741 tgttttagcg tccgtacctg acattttgaa accaccaaat ggatggtatc caacaactgc
    10801 tgaagtacaa cctctgttaa ggtataaatt acctacatca aattcgttta ccgctttaat
    10861 ccaatgctcg cgattatttg taatcactgc accagttaaa ccgtaatctg tatcatttgc
    10921 aacctcaatg gcttcatcaa aatcgttaac tttcacaaag ccaacaactg gaccaaaaat
    10981 ttcttcttgc atgattctat ctttagattt aagtcctgaa atgattgttg gttctacaaa
    11041 gtaacctttt gaatcatcag tgccgccacc ttgttctaat ttaccttctt ctttaccaat
    11101 ctcaatataa tttttaatct tatcaaattg ttttttatta ataactgggc ccatatacgt
    11161 attgtctaca gtattgccca acgttaattc ttttgttaat ttgattgatt tctctaatac
    11221 ttcgtcataa acgtctttat gcacaattgc acgtgaacat gcagaacatt tttgaccaga
    11281 gaaaccaaac gctgacgtta caatagcttc tgccgccata tctgtatcaa tattttcatc
    11341 aactacaatg gcatctttac cacccatttc agcgataaca cgtttcaaga agttttgacc
    11401 ttcttgaact acggcactac gttcataaat tctagtacct gtcgcacgtg atcctgtaaa
    11461 tgtaacgaaa tgcgtatctt tatgatcaac taagtaatca ccaatttctt tcggatcacc
    11521 aggaacaaag ttaactacgc cttttggtaa tcctgcttct tctaaaattt ccattaattt
    11581 ataagcgata taaggtgtat cctcagcagg tttcaataac actgtattac ctgccacaac
    11641 tggtgctaaa gttgtaccag ccataatcgc aaacgggaag ttccacggcg gaattgtaac
    11701 acctgtacca attgatttat agaaatattt attgtgttca ccttcacgat caagtactgg
    11761 cttaccttga gccaagtcca tcattgaacg tgcatagtat tcaataaaat caataccttc
    11821 agctgcatca ccaactgctt catcccatgg cttacctgct tcataaacca taattgctgc
    11881 aatttccgct tttcgacgac gaataattgc cgaaacacgt aacataagct ctgcacgatc
    11941 atttgctgac catgttttcc aagatttata agcttcgttt gctgctttaa acgcatcttc
    12001 aacatcttgt tttgttgcct ttgatgcatt tgcaatcact tgtgatgtgt ctgcaggatt
    12061 gattgattta attttgtcat ctttgaaaat cttctctcca ttaatcacta atggtatgtc
    12121 ttgacctaat tctttttcca cgtctttcaa tgctttctta aacatatcca cattttcttg
    12181 gactgaaaaa tcgtaaccag gttcattttt aaattctact accatgtaca cttaccccct
    12241 ataaattttg aaagtggttt aaccctttga tttaatgata taacatcatt taaactcatt
    12301 ttactatgat taaggttagt tttgcaatcg ctttcatttt tatgctttat cacttattct
    12361 caagtatttt gaaattgatt ggttactttt taaaatttat atgggtcgca actgctactt
    12421 tatcgtttcg tcatttaatg tttcggatgg taggtcatta tcaattttac gaacgacttt
    12481 acaagggttt ccaaccgcta agctgtgtgg cggaatatct ttagtgacaa cactaccagc
    12541 accaatcaca ctaccttctc caatcgtcac ccctggtagc acggctacat gaccgccaaa
    12601 ccaagtatta ctgccaatat gaatgggtcc ggctttttca aaaccttcat ttctatgatg
    12661 aaaattaagt ggatgtgtcg ctgtgtagaa tccacaatta ggtccaataa aaacattatc
    12721 gccaattgta atctgtccac catccataaa ataacaattg gtgtttacat agacattttt
    12781 tcctagttta acgttccaac cataatctgt atcaaaagga atcgaaatac ttacattgtc
    12841 tgttgttgtt tgaaataatt gatcaattaa ttcctttctt ttatttgtag cactcggtct
    12901 tgtatgattt aattcaaagc aaatatcttt cgctcgtgca cgttcattga ttaagtattg
    12961 atcaaagttt gcatcgtacc atttttctgc taacattttt tctttttcag tcattacacc
    13021 tttcaactcc taataactta tttacttgtt taaaagttaa tcaaataaac attcgcctat
    13081 gcaactaata cgctataaca ttatgaaatc atgaccttat cacccttatc tatacaattc
    13141 tcgcatcaaa tactgctaaa gtagtagata aattcaatac tacagacgca ttcatttttt
    13201 aatctattaa cgtacaatgt gagtaagaga aatataaagg agtatgatag cgatgagaat
    13261 attaattaca ggcacagttg ctatcttaat cattctaggt ttagttaaaa cgatacaaga
    13321 ttacgaaatg acaaacgata caagtcgtca gttgtcagac aacaaagatg atgataaagt
    13381 catccatctt aataatttca aaaatttaca tgcgaaagaa tttaacccat ctgatttctt
    13441 ttaagtcacc taagaattgc aaatccagaa gtcatttaag ttttaccttt cattcataca
    13501 tcctttaata ttaattacga cttcttttat atagatgcta aatagagaga ttgttgtgca
    13561 atgtttgcac ggcaatatct cttttctttt taaaattggt aaaagtaaaa cgcaacgatt
    13621 gacttatata cctatagggg gtacattaga cgtgtaacaa tgaatcacag ggaggcaata
    13681 atgtggctaa tacgaaaaaa acaacattag atatcactgg tatgacttgt gccgcatgtt
    13741 caaatcgtat cgaaaagaaa ctgaataaac ttgatgacgt taatgcccaa gtgaatttaa
    13801 ctacagagaa agcaactgtt gagtataacc ctgatcaaca tgatgtccaa gaatttatta
    13861 atacgattca acatttaggt tacggtgtcg ctgtagaaac tgtcgaatta gacattacag
    13921 gtatgacttg tgctgcatgc tcaagccgta ttgaaaaagt gttaaataaa atggacggcg
    13981 ttcaaaatgc aacggtcaat ttaacaacag agcaagctaa agttgactat tatcctgaag
    14041 aaacagatgc tgataaactt gtcactcgca ttcaaaaatt aggttatgac gcgtctatta
    14101 aagataacaa taaagatcaa acgtcacgca aagctgaagc gctacaacat aaattgatta
    14161 agcttatcat atcagcagta ttatctttac cactattaat gttaatgttt gtgcatcttt
    14221 tcaatatgca tataccagca ctatttacga atccatggtt ccaatttatt ttagctacac
    14281 ctgtacaatt tattattgga tggcaatttt atgtaggtgc ttataaaaac ttaagaaatg
    14341 gtggcgccaa tatggatgta cttgttgctg ttggtacaag tgcagcatat ttttacagta
    14401 tttatgaaat ggttcgttgg ctaaatggct caacaacgca accgcattta tactttgaaa
    14461 caagcgccgt actaattacc ttaatcttat tcggtaagta tttagaagct agagcgaagt
    14521 ctcaaacaac caatgcgctt ggcgaattat taagtttgca agctaaagaa gcacgcattt
    14581 taaaagatgg taatgaagtg atgattcctc taaatgaagt acatgttgga gatacactta
    14641 tcgttaagcc aggtgaaaag atacctgttg atggcaaaat tattaaaggt atgactgcca
    14701 tcgacgaatc tatgttaaca ggtgaatcta tccctgttga gaagaatgtt gatgatactg
    14761 taattggttc aacgatgaac aaaaacggta ctattactat gacagcaaca aaagttggcg
    14821 gggacactgc gttggcaaat attattaaag ttgtcgaaga agctcaaagt tctaaagcgc
    14881 cgattcaacg attggcagat attatttctg gttatttcgt tcctatcgtt gttggtatcg
    14941 cactattaac atttatcgtg tggattactt tagttacacc aggtacattt gaacctgcac
    15001 ttgttgcgag tatttccgtt cttgtcattg cttgtccatg cgcattagga cttgctacac
    15061 caacttctat tatggtaggt actggtcgcg ctgctgaaaa tggtatttta tttaaaggtg
    15121 gcgagtttgt tgaacgcaca catcaaattg ataccatcgt tttagataag acgggtacca
    15181 ttacaaatgg tcgtccagtc gtgacagatt atcatggtga caatcaaacg ctacaactgc
    15241 ttgctactgc tgaaaaagat tctgaacacc cattggcaga agccattgtc aattatgcaa
    15301 aagaaaaaca attaacatta actgagacaa caacatttaa agcagtacct ggccatggta
    15361 ttgaagcaac gattgatcat caccatatat tggttggtaa ccgtaaatta atggctgaca
    15421 atgatattag cttgcctaag catatttctg atgatttaac acattatgaa cgagatggta
    15481 aaactgctat gctcattgct gttaattatt cattaactgg tatcatcgca gtggcagata
    15541 ctgtaaaaga tcatgccaaa gatgctataa aacaattgca tgatatgggc attgaagttg
    15601 ccatgttaac tggcgataat aaaaacactg ctcaagccat tgcaaaacaa gtaggcatag
    15661 atactgttat tgcagatatt ttaccagaag aaaaagctgc acaaattacg aaactacagc
    15721 aacaaggtaa gaaggttgcg atggttggtg acggtgtaaa tgatgcacct gcattagtta
    15781 aagctgatat cggtatcgcc attggtacag gtacagaagt tgccattgaa gcagctgata
    15841 ttactattct tggtggcgac ttgatgctta ttcctaaagc catttatgca agtaaagcaa
    15901 ccattcgtaa tattcgtcaa aatctatttt gggcattcgg ctataatatt gccggtatcc
    15961 ctatagctgc attgggctta cttgcgccat gggttgctgg tgctgcaatg gcactaagtt
    16021 cagtaagtgt tgtcacaaac gcacttagat tgaaaaaaat gcgattagaa ccacgccgta
    16081 aagatgccta gattccttaa taatgaagga ttcgttggtg attctgagat aggctagtga
    16141 ttggctctat aatgtcgcgg tttacagttg gatcttcgct ccaactgcat aagagccact
    16201 aattattata aataataagt ggctctaaaa ggaggtgaat gctatgtcac aagaaatttt
    16261 aaatgttgaa ggtatgagct gtggtcactg caaaagtgct gttgaatctg cattaaataa
    16321 tattgacggt gtcacttcag ctgacgttaa ccttgaaaat ggtcaagtaa gtgttcaata
    16381 tgatgacagt aaagttgctg tatctcaaat gaaagacgca attgaagatc aaggttacga
    16441 tgtcgtttaa tcaggcaata ttcaacgtca tcaacaccaa attaaaaaat cgaactgatg
    16501 agaatcccaa caatccaaat tatctcatca gttcgatttt taatttactc gtaacctagt
    16561 atctccagtc tgcaatacat ctaatgttgc atctaatgca tcgacaatta gatttttaac
    16621 tgcagcttca gtataaaacg caatatgtgg tgttaatatg acatcttccc tgtcaatcaa
    16681 cgattctaac aatggatcgt tcagtgtttt gcccctttga tcacttggga aaagtttgcg
    16741 ttcaaattca tacgtatcaa gtgctgcacc tttaatcaca ccattgtcta atgcgtctaa
    16801 taacgcctta gtatctacta aagaacctct cgcacaattg acaaatactg cgcccttttt
    16861 aaaatgttta aataattcag cattaaatag ataatgatta tatttcgttg caggtacatg
    16921 taatgtcacg atatcagcac cttcaaccgc ttcctcaatc gtatctttgt aatcgacata
    16981 cgttgcaatt ttagcattag gaaacgggtc gtatgcgacc acatcacttt gataaccatt
    17041 ggcaaatata tcggctacta cacggccaat tcgacctgta ccaataacag ctacttttaa
    17101 atctttaatg gatttcgata aaatagtagg ttcccatcta aaatcatgct ctcgcacttt
    17161 cgtttgaatt tgattaaaat gacgaaccac attaatagcc tggttcacag caaactccgc
    17221 aattgaattc ggagagtatg acggcacatt tgacacaatc aagttatact tgtttgctaa
    17281 ctccaaatca tatgtatcaa atccagcact acgttgtgcg atttgtttaa tacctagttc
    17341 atttaatcgt ttataaacat gctctgataa tggtatttgt tgtgatagcg ataagccatc
    17401 ataaccagcg acaccttcaa cattgtcatc agttaatgct tctttagtaa tatctacctc
    17461 aacatgatgt ttctctgccc acgccttgat ataaggcata tcttcatcac gtacactcat
    17521 gattttaatt tttgtcattt taacatcacc cttaacttta ttattcatat aaatatgcta
    17581 gttctgttaa tcttattgca gcttcgtcta atttctggtc atctaacgcc aatgaaattc
    17641 tcacataacg attaccattc tctccaaatg gtttccctgg agcaacaagt attgacttct
    17701 cttgcactaa aaattgctca aattgctcgc tgtcataacc aggcggtgtt tccaaccata
    17761 catatatgcc acctttagca tgaacaaatg gcaaatcagc ttttgcaagc atggcttcga
    17821 atcggtcacg acgtgtttta aatacattgc tttgttcttc taaaaaatca tcataatgat
    17881 tcaaagcata tattgcggca tcttgtaatg caccaaacat cccagcattt gtgtgcgttt
    17941 ggtacttttt caaagcttga atcatatctt tattaccaac tgcaaaaccg actctaaaac
    18001 ctgacatgtt ataaccttta gacaaggaat aaatttcaat cgcaacatct ttcccatttt
    18061 ccgaagcaag tatactagga tttttagcgt cgaaaccgaa agcaccataa gcaaaatcat
    18121 gcacgatttt agtgtctgta cctttaaatt tagctatcgc ttcatcaaaa acttctttcg
    18181 tagctgtcga tccagttgga ttatttggat acgttaaata aatgagtttt gttttatcta
    18241 ttatttgtga atcaactttg gaccaatctg gcaaataatg tggcggttct aaattaagcg
    18301 gaactggctt gccatcagct aaaagtacac ctgctaaata gtccgtgtag cctggatcag
    18361 gtagtaatac atagtctcct ggattgataa cacatgttgg tactgccact aatccatttt
    18421 ttgtaccata taaaatgcat acttcatctt ctttatctaa cgtcacatta tattgtcttt
    18481 gataaaaatc tacaatagct tgcttgaacg cttctttacc atgaaaagca ccatattttt
    18541 gattttcagg aatagttagt gctttttgaa aatgatcaat aataccttgt ggcgtgggcc
    18601 catcagggat cccaactgcc atattaatta atggcaatgg tccatgttcg attttacgtc
    18661 ccatcgtttt cccgaaataa ctatcaggga tatttgctaa tttgttagag atcatcaaat
    18721 tcctcctcta tcattaaaca tagcctgggc gactatcata atcctaacaa cttgtatcac
    18781 tctcatttag atggttacaa tgacatcgcc attcaccgtt atgttcaaca gaacttatga
    18841 cacacgttgt attgaatgaa tttattttca ttttaggtag gtacattatt attgtcaata
    18901 ttaggaattt tcagattaat atgcactcaa tcgttatgat ttaactgtca tgcatatccg
    18961 catgcgcaac cagttagata tgcttatata aagtataacg cccctcaagg tacgtattca
    19021 aacgtgaacc ttaacaggcg cctattcatt gttaaataaa acttctttag aacatactta
    19081 tttcactatg ccttttacgt tccccttata cttttctcac atctttctct tagactactc
    19141 ccttatacgc cccgctcaat atctttaatc atttcgtcta cagttatttt cgcacttgtt
    19201 aagacaatag gaacgcctgc acctgggtgc gtacttgcac ctgcaaaata taaatcttta
    19261 taatctcgcg atacattttg tggacgataa taattacttt gcgctaaagt tggcattaaa
    19321 ccgaatgccg aaccaaattt cgcatgatac gtttgctcaa aatcatttgg cgtaaagatt
    19381 gtttctgaaa caatatgcga ttttatatct tcaaatactt caattgttgc taatttacga
    19441 taaataattt cctttatttg ttgcgtcaaa gcttcatctg accaatcgat tccgctacct
    19501 gttttaagtt ccggcgtcgg cattagcaca taaataccag ttttgccttc tggcgcaagt
    19561 gatttatcag cgaccgctgg tacatacaca taaatagaag gatcatatga taaacgtccc
    19621 tcaaatattt cttcaatatt gcctctaaag tcatctgaaa aaataacatt atgaagtctc
    19681 acttgatctg tcacatcaat atctataccg atatacatta aaaatgctga acaagagtaa
    19741 tctaagtctg caattttatg tggtggatac tttttaatag gtgcaaaatc tggcattaat
    19801 gattccgcaa cactagggaa atcagccgta cataaaattt tatcaaattt tcttatgtca
    19861 ccattcactt ttatcgcatc ggcccgtttg aatttagaat caataataat ttgctcaatt
    19921 tcagcattta gttcaatatt aacgcctaag tctttattta attgcgctag cccttgagcc
    19981 atgccataca taccgccttt aataaaatgc acaccaaaca tcatttcaat cataggaata
    20041 attgaatata gtgacgggcc tcgttttgga tcaattccta tgtataacgt ttgaaacgct
    20101 aaaagctttt gtatcttttc gttatcaata taatgttcaa ttagctgatc tgcatgattt
    20161 aacgttttta acttagcacc ttgcacaagt gacgtcatat tataaaagtc actcggtttg
    20221 cgatacgttc tttctaagaa atagcgacgt gcaatttcat attttttata aacatccgtt
    20281 aaaaaggaca taaaaccatg cgttgaacca ggttctatac tttctagcat ttgctgtaat
    20341 tcagctaaat ctgtaggcac cgttatacga tcatcgtggt caaaatacac atcgtaaata
    20401 taacgtaatt gtctcaattc aatataatct tcataatttt taccacacgc tgtaaaaaca
    20461 tctttataaa catctggcat catgacaatt gtgggaccca tatcaaatgt aaagccgtct
    20521 ttctttaatt gattcatacg cccgcctaca ttattatttt tttcaaatat cgtcacttca
    20581 tgaccttgag aagcaatacg ggctgccgct gctaatcctg tgacacctgc accaattact
    20641 gcaatcttca ttattcaacc acctatattc tatgatattt actatttatt tcatgaaaca
    20701 actttgcctt tttccgctta tccacaaaaa cacgttcatg taatgtatag ttagcctgtc
    20761 tcacttcgtc cagtatttca atatatatac gtactgctaa ttctatgatt ggttgtgctt
    20821 caatactaaa tactttgatt tgatccataa catcttgaaa atctttttct gcgatagctg
    20881 cataatattc ccataagtca atataatgat tattaacacc attttggtac acttcagcaa
    20941 tatcaacttc atattgcttt aatcgttgct tactaaaata tatccgttca ttgtcaaaat
    21001 cttcaccgac atctcttaat atattaatca attgcaacga ttcaccaagt cttcttgcga
    21061 catcgtatgt ctgatgtgtt tcatgatcac ttaaaatcgg cgtcaatact tcacctactg
    21121 taccagcaac accataacaa tatccgaata attcagcgtc cgtttcaaac attgtaaaat
    21181 gttgatcttt atatacagta tcaatgagat tataaaaaga ttgaaaggcg atatttttat
    21241 gttgtgcaac atgctgaagc gccatcatga ttctacgatc actttgaaag tgatgatgtt
    21301 catatgggta tttttcaata gattgtatat cttcttttat ttgatttaaa aattgaatat
    21361 cgccataaac atctatactg tcatcaattt tacgacacac agcataaatt gcccaaaccg
    21421 cttttctttg atcttctggt aacaagtcaa aagcgtaaga aaagcttttt gaatgtttct
    21481 tcatgatttt atgacaatat ttaaaattca tatccatcat tgtcatacta gtcctcctat
    21541 attgaaatgc cattaccttg atttattgtt ctttagatat agaatattga cgacctttcc
    21601 attcaactac accataacga tgcgtttgtt tccaagattg gatgaaaatt ttagtaaaaa
    21661 acataaataa caatggatga cataccatta ataaattaga aaattggccg atccttcgat
    21721 gcagataaat aaattgtgta gtatagctca agtaaagtgc taccattttt cttacagaca
    21781 tttggcgata ttttaaacta agacatagcc ctaaaatact cgctatagaa ccaaacaacc
    21841 acaacacaat tgctgtcatg atcttaggct ttgtgccacc tgccccagtt gacaaatgct
    21901 ttgtccatcc ttcttgtaat gactgaaatc cttcttgata catgcgaaat gcaacaaatg
    21961 gaaacccttc ataaactgtt acgggcaatg attgtgaagt atatgcactt cctaaagcaa
    22021 atccttcaat aatatgacgg tttgcacttt tatgacctcc agttgcataa taatcttctt
    22081 tatttgttaa tgtcaccggt ccaaatgcat gctggttagt ccgaccgtcg tctaaggtag
    22141 aaaatacatt cataccaacg actgtcatta aattaaatat cgctgaaaac ccttcgtaga
    22201 aacgctttgt tatatgataa ggctgtacgc ttaacaatcc tttttcacct tgtaattgat
    22261 actgattaat caacgtttca acagcatctt tccttaagaa agttacatca gcatctacaa
    22321 aggcaatgcg attcgtacat gcatgcgtca caccttgata acaagcatgt gatttcccat
    22381 accatttgcc gtcagtatca tcaacaacat ctactacagt cgcaccatat gaacgtgcta
    22441 cacgtgctgt ttcatctgtc gatccgtcat tcataacaat gacatctact ggaacttgct
    22501 gttgtattat cgaatgtagt aaatgaccta ttcttttttc ttcgtttcta gccggaataa
    22561 taattgttaa tgctttatga ttgaagttca gcgttttcgc ctttagctga tgtctacgat
    22621 taaatatcaa tgcaccacac gccatagaca tggtcactat tactgttaat attcgtgata
    22681 accatttcat agatatcact tcctattctt cgcttctcgc gcgtttattt tgtctgcgac
    22741 ttgttgccca cttaatgtaa ccattggcat gccaccacca ggatttactg atccacctac
    22801 aaagtacaag ttttcaaaat actgactttc tttaggaaat ttaaatcctt tgtttttctt
    22861 tttatctgct tcaacaccat atattgcacc acgattagaa cgataatttt tttcaatatc
    22921 ctccggtgtc caaacatctt cataaataat gtgttttctt aaatcagtaa gtcccatttt
    22981 ttctaattta tccaaaattt tatccctaaa cttcgcataa tcttcagtgg taaaaggctg
    23041 atcttgaata tatggaatat gtggtaagac tttgatattt tcataaccta ctggcgcttg
    23101 tgtgtgatca gttttattcg tatttactag ataaatggtc gaatcatctg gcaatacctt
    23161 ttcatgaaaa acttgttgat aattgagata agcattttcc gtaaaaaaga aattatgatg
    23221 tgctaattgc gggtattggc aagcaacacc taaatgcatc acatatcctg agcttgctgg
    23281 ctcaaattcc cgctctaatt tgtttaatcg ttgagtatca aggtgaagta aatatttata
    23341 agtaggtatg acttccatat ttgaaataat ataatctgcc tttacaaact cacctgtatc
    23401 taatctgaca cccgtcacac gtctttgata tgttttaata ttgtccacac gtgcacctgt
    23461 atgaattgtg acaccttctt cacgcgctag cttttccaag gcattggcta aatgatggat
    23521 tccaccttct acataccaaa ggccttgctc ttgttgcata tggaataaca tagataatac
    23581 agctggcgca tcgtatgatg aagaacctac atatttgata aaatagccta acatttgtcg
    23641 caagtatgga ttcgagatac gcttgtctat ggcctgttgc atagtatgta cataatcata
    23701 attaataaga gcatttaatg gcccatgaaa tttaataatt tgagatagtg tatctaaacc
    23761 atagttaaaa tacccttttt cagtaatacg atcgattcgt cttgtataat tcaaataatt
    23821 ttgcagttcc tctatatcct gtttcgacaa tatcgcatta tgctgacctg tttctttaat
    23881 accttcatac aaatcgatag tcgttccatc tggaaaaaag ctacgccatt gatgtggcaa
    23941 tcgcttgatt gtaacgtagt ctgacatttg cttcttgcta tattcgaata atttttcaaa
    24001 aatataaggc atcgttaaaa tagatggacc taaatcaaaa ccaaagccat ctgattcatg
    24061 acgattcact ttgcctccta tatgattatt ttgttcatat aatgagaccg aatagccact
    24121 ttgtgccatt cgaattgctg cagaaatccc acctaagcca ccaccaataa cgatgatatg
    24181 tttagtcatg acgttcacct cttcgtttta atatgcgtag taactgcgtt aatctcggtc
    24241 gattatagcg ttgcacaata atgattggaa cattagctat cattgcatag aaaatattta
    24301 tatactttac taaacgaggg cctttattga atatgatgac tggaagtatc gatatccaat
    24361 gaatcaactc tgccctcttt gtctctataa tcattttttc aatttcatca gtattgattg
    24421 tcattaaatg acgctgatca tatatatttt gattaagctg atgaccatct aaaatcttat
    24481 gtttccattt tcttacgtaa aaccatttgt tccaaaattt tccatgcttc tcaaagttaa
    24541 atgatttaaa tattatgtac ttctgacgaa aatacttatc aggaattctt gtacctaaat
    24601 ttgctatatt tagttgaaca attaaccaat acatactaca aaaaaatgct gttttaatat
    24661 attttttcat ggttttcatc taaattgaat cactctcaat catactgaca tagtataaat
    24721 tatcacttta ttacttagtt ccctaaagat cagtgaataa accgctctca tttatattta
    24781 tataaaacaa aaacgccaat actctcatgt tagagaatat tgacgtcatt tgatttacat
    24841 ttgattaatg aatgaaatta tatgaacctg cttggtttgc tgaaattgta cgagacgtaa
    24901 ccacaccagc accatgtcca tagttcattt ctgaaacacg aacagaaccg ttgctgttaa
    24961 cgccttcaac gtaagcaaca tgaccgtaat agccttgtgt tgtttgcatg atagcaccaa
    25021 cttttggtgt attgttcact gtatagccag atgatgcagc tgcgttagcc caattacttg
    25081 cgttacccca tgttgaacca attttcccac caacacgatc aaatacataa taagtacatt
    25141 gtcctgaagt atataagtta cttcctgatg cataaccatt agaaattgaa cgaccatttg
    25201 aagatggcgc tgcagttgta gtcacatgaa cattattact tgttgtgcta taacttgcac
    25261 ctgaaccacc agtataatag ttatttgttg ctgtttgact attgtttgta tagttattgt
    25321 attgatatga atgattatag tagttattgt aagatgcatt gttataacta taagtattgt
    25381 agttgttgta gtagtatgtg ttgttttgcg ttaattgact tggattccaa tttcctgtcc
    25441 aagtgtaatg ataattacct tgtgcatcaa ttgtatacgt atagctgtat gattgagcgt
    25501 cattagaatt atatccattg ttattttgtt ctgcggcttg tgcatcatgt cctgcaaatg
    25561 cgatagtggc aagtcctgct gtagcgattg tagctgtaac gatttttttc ataattaaat
    25621 atcctcccaa taatcaaaac actctataca tatagtcatt agcgactcaa ttattttaaa
    25681 ttttatattt tttatttttt tagtatgcaa acaattataa cttttatagt tttgcgacaa
    25741 aacaaactat aacagagatt aggtgtcttg cgggctttgt tatcataatg taatctgttc
    25801 ctaataaagc ttcattgaat gtaatatacc aattagaatg tttacaacac tttttctgca
    25861 ccgttactat gggatttgaa aataaaaaag cactaaactt ttaatgaaat acatcatttt
    25921 tgataataaa tatcagaagt aatgtaatat ttcaaaagtt tagtgcatca aattatttct
    25981 tatttgtagc atgtgtttcc atcgttttta caatcaaatc agtcagcgct ttactacctg
    26041 catattctaa gtgaataccg tcatatgcaa agtattccgg atgacctgca gaagctttat
    26101 accaatcgac tagatgtaca ttagagcgct tttcagctgc ctcataaatt aatttattta
    26161 ttctaccttc ataatctcta ggtactctaa tagaaactaa atatatgtct gcttttccaa
    26221 aactatccaa tagttcattt aattgatctt tcgtaaatgc cccatttgta ccaagctcta
    26281 ctacaacttt ttgacctttt ttagcatagt ctttatattg cgatttcaca attggtgtag
    26341 catcaacgag ttgccgtcca actttaccat caatttgtgc atttggtatt ttcttagtaa
    26401 agacattacc aatatccacc atgaccgagt caccaattaa tagtggtgat gactttttaa
    26461 tatcctcttt gctattcgct gtctgcttat cctcttgctt atctttttta acgacttttt
    26521 tcttcgttgt tttaaattcc gtcttcttct ctttcttctc agcttcatgt tgtttgccaa
    26581 gtgcatcaaa ctgtccactg agcacaacga tagacggaac caataatagg ataactaaca
    26641 ctgttctagc aaattgcccc ttctttttag gtaaaaatgc aaaagcttta aatccttttt
    26701 tacgtatagg tgtttcaata aagcgatacg aaatttcagc cattaacgct gttaacaaaa
    26761 tttctataat ataaacgtat accggtattt gtccttgtac gtaataactg ttcacaaaaa
    26821 caatgatagg ataatgccat aagtataagc tatatgatcg tttacctata attagtaaag
    26881 gtttcatact taaaaattta gcaaataaac tagaaggatg taccgcaatt gcaataatga
    26941 ataaagttgc aaatgatata atgtaaaatc ctccattata gatccattga tcttggtctc
    27001 caactataaa gaacaaagtc attagaaccg caaaaccaga tatccctata atatctaatg
    27061 atacgacaat ctttttagaa atatcttttt tcaaagcaaa cggaggccag ataaatgcta
    27121 atatacaacc aagcaataaa gtttgcagtc gtgtatctgt cccaaaatac acacgtgaat
    27181 tatctccagt gatgaaatga atcactatca taagtcctaa agaaatcaac gatacaataa
    27241 atagcgtttg aataatattt ctcggtttaa atctatgtaa taagaacgtg ataaccaatg
    27301 ggaaaagcaa gtaaaattgt tcttcaatgg ctaaagacca taaatgtttt agtggttcaa
    27361 tagcaaattg gttaaaataa tctacattct gtgagatgta ccaccagttt gaaacataga
    27421 atatagctgc aatagcatct cgtttcattt gtataatcaa ttccggttta aatatcagtg
    27481 tgaacgtaag cacgacacaa attaaaaaca acactgccgg aatgagtcgt ttcaatcgtc
    27541 gcttccaaaa ctctagcaaa tcgatttttt gcgtccgata atactcactt atcaacaaac
    27601 ttgttattaa ataacctgaa ataacgaaga atgtatctac tcctaaaaag cccccactta
    27661 accattgtgc attcaagtga taaataatga ttcctataac tgcgaatgcc ctcaatccat
    27721 ctaatccggg taagtatcgc ggggaataca ttttttctaa acgtttaaag tcttttgtat
    27781 ccatgttaat aaacgcccca tttatttttc tctattttgt agcatatcac aatatttttg
    27841 aaaataaaat attgcactga ttttcattaa ttgatttaac ccttaattaa gatagtttta
    27901 aattttttat taagtagaaa acaattatta cagttaattt cattactgca aaccacatat
    27961 aaatttgtcg attttactac ataacataga ttatcataga ttcttgaatt tttagcaaaa
    28021 taactgttat tttcattata tttttacaaa aaaaggttcg ttttatattt tatgcatctt
    28081 actgtaacag aatcattaag atatgctatt cgaatatact tttaaaaagt tgaataaact
    28141 gataaactac cgcgcactgt tattaaaata tatccacggt gatatctttc caactcactt
    28201 tcaaatttaa ccatatgaaa tgacaaatac tcactccata ttgaagtatt tgtgtaaaaa
    28261 gaatcatggt gtattgttta tttcgcctta tctaatttaa tagtgacatt aattcttcta
    28321 tcatgacatt aaaatcgtca ggaataataa aataacatcc taacctctag aatcagactt
    28381 taatttctaa taaaaaaact ctccccaatt tctatgggaa gagctatata tttaatgtct
    28441 aaacattact tttatttatt atgaaggaat tagaatcccc aagcgcttaa accttgtgct
    28501 ttgtatgctt taacagctgc gttgatttgt tggtcaacag tgtttgttgg accccaacct
    28561 ggcatagttt ggaataaacc tgaagcacct gatgggttgt aagcatttac ttgaccattt
    28621 gattcacgag cgatgattgc agcccatgta gaagctgaaa caccagtacg ttgagccatg
    28681 atttgagctg ctgatgaacc agtagcacct gcagtattac cattgcttaa tctcactgaa
    28741 cttgaagtag ttgaagtgct gtagttatgg taagttggag ctgaaacagc ttcaacgttt
    28801 gagttacttg attgtgcatt gtagcttact gattgtacat ttgaaccttg gttgtatgaa
    28861 gtagtgtagt ctgcacctgc aacgtttgag aaaccagcag tttgaccatt agctgcttca
    28921 tagctccatg accatgtagt accatttgaa gtaaagttat attggaaacc atcttttaca
    28981 aagtggatgt catatgcacc atctttgatt ggagctgcat ttaattgatc ttggtgatta
    29041 tgcgctaagt caactaagtg tgcttgatca acgtttactt cagcagcgtg tgcttcatgt
    29101 cctgtgcttg ctgcgtaacc tgttacacct aatgccactg ctaatgatga tgccataatt
    29161 gtctttttca tagtaaaaaa tcctccagta ataattgtaa gtttatgttt ttagtaatta
    29221 tattttgaat ttgaatgtcg tagtgcaagt ttaaattgtc ttttatttct ttcaacggta
    29281 ctcactatat cataaaaaac cagccagtaa attacacttt ctttacaaaa cattacaata
    29341 tcaagtttta tttgtaatgt tgaaatatgg ctgttttata ctgtaatgtg aaatatgttc
    29401 cctttagaat ccaatcaacc cttgaaatag tctttaacac ataagatttt tactatgttt
    29461 agctcaacta ttacaacttt cgtaatatta cagattgtat ttttgttaca tagctgtaat
    29521 atatctgaca tgtaacttct cttattttcg tacattaaac gaattaaaaa gcaatcaaca
    29581 catatatttc tacaaatgta ttgattgcta ttattgttat atattcaaag ttttaaaaca
    29641 cacatctttt gtgaattgtc ttatctttta ttagcgcaaa taaactgcaa ctcaattata
    29701 ttgctaaact ttattctcgc aattcacaat aacattaaat aatttttggt ctaatatttt
    29761 caaaaaacat actgtcctta tcccatgaat ttaaaaatat cattagtata taaacgaaac
    29821 actttacgat aaatgatatc tgcaagccaa gctgttacaa atggcacaac aaagaacgct
    29881 actacaatta gtatgacact caaccaagca gaatcaacct ccataaattt aaatgcatta
    29941 atcggtccta ccattcctat aaaaccaaat ccagctgact ctttcgttcc atgaatacct
    30001 actaatgctg ataccaaacc tgatacaatg gctgttgtca atattggtaa cataagaatt
    30061 ggatatttca ccatattagg tatcatcatt ttaacgcctc caaagaagac agataacggc
    30121 acccctaaac gattcacttt acttgtacca attatcaata ctgcttcagt cgcggagata
    30181 ccaattgacg ctgatccagc tgctaaacct gtaataccta tcgcaaaggc aatggccaca
    30241 gttgatagtg gagaaataat aataagacta aataccattg aaatcaaaat actcatgaca
    30301 atcggttgta attctgtaaa accattaacc atattaccga tggctgttgt aatcattttc
    30361 gtatacggca atattaaaac accaattgca cctgaaatac cgccaacaac tgttgggaat
    30421 acaatcaatg ccatactacc tacgcgatgt tgaataagta aaatgaacaa cactgcaatc
    30481 gctgctgtaa tcattgtatt aattaaatca ccaatacccg taatcatcca agcaccattt
    30541 ttaaactgcg ctgcaccgct tcctacatat gctgcacttg ccacaacagc aattgctaat
    30601 ggcgataggt caaatttcat ggcaaccaat gcaccaatca aagcaggtac tgtaaattga
    30661 attgcaacga caacgcctaa taacgtttta aaaatcggat gataatccat aaagtattta
    30721 aaaatttctc caagtatcgc attaggaact aagcccgcaa caatacctat ggcgacacct
    30781 gataaaactc taaatataaa atctttgggt gtaattgttt taattgatgt cataatatca
    30841 tccttccatt tatgtatata catctttatg caaataataa agagccttaa gttataagct
    30901 gccactagct taaattctaa gatgtgcatg ccgatgttgt tatatttagg ctagcagtat
    30961 catctataac tcaagactat gaaaaatagt atatcacaaa attctgaatt tttagataaa
    31021 gaaattggca atttttcaaa catattgtta caatacactt ttattttatc ttcattttta
    31081 aaatccatta atataataga agaaagacat tcaaatgctt accaaaaagg tacattattt
    31141 gttaggagcg tatcagcact tacatatcat caacacaatt gacaacataa tagaagatac
    31201 tgataataag tgttaaaaca acagatgtta ggtagtgaac aaatgatgga aagtaaatcc
    31261 atagatccaa gaatcgttag aaccaaacaa ttgcttgtcg atgcttttct taaaatttct
    31321 agagaaaaga aattatctca aattacagtt aaagatatca ctgatattgc tacgctaaat
    31381 cgtgcaacat tttacgctca tttcactgat aaagaagacc tcctagacta cacattatct
    31441 gtaaccattt taaaagactt gaatgataac ttgagcattt ctaatgtcat taatgaaaag
    31501 gtgctgcgta atattttcat ttcaattgcg agttatatca aagatgctgc aaagtcttgc
    31561 gaattaaata gtgaagcatt ttgcaacaaa gcacatcaac gtattaataa tgaattagaa
    31621 gatatttttg cgattatgtt agaaaacagc tacccagagc atcaacgcga tatcattgta
    31681 aatagtgcga gttttttagc agctggtatc tcaggcttag cattacattg gtttaacacg
    31741 agtcaagaga cagccgatgt gtttatcgat tgcaaccttc catttttaat tcatcatata
    31801 gcacattttt aataaaactt ggtatttagt catgcatctt gaaatcacta tgtgacttag
    31861 gttcatactt gtacacacaa taaaatttaa cgtattacga ttgattagcc gtgtctagga
    31921 cataaatcaa cgtcctatac tctacaatgt catattagca gtcgttaact gaatgaaaat
    31981 aagcttgtca ttaaaacata tagattttag tgacaagcat ttttgttttt gcgtacttaa
    32041 acaacacttc aggcaatatg ttgtttaggc aacaaatgat atgtgcgtgt ttattggcaa
    32101 acgtacgaca tagtagtata gtatgtctaa acaacatatg ttgcatagtt gatatgcgtt
    32161 gtttaaatac taagatagga gggattgacg tgagcgagac agatgaacct caggggtttg
    32221 aacgcacgca taatatatta aatattaatc agagtagtct gggtgtagtg acatacatta
    32281 caaataaatt aaagtcgacg ttgaagcaac acataataat tgctcgtggt aaaaagcgaa
    32341 tcgactatcg actgtcgtat aacttttaca ctacgtatta tgataatgta gaaatcaaca
    32401 aaatcgactg tgaatatacc tatgctatgc ccattgcaat tttaataaga cacacgatgt
    32461 cattcgacaa tgctcatttc tttgctcagt tacgtcatcc tgtcttataa aacaacattg
    32521 cagacatgta tatcaaacga cacttcaata acatcacttt gccatcgtac tactagtaaa
    32581 atcgtgtctc aaatccctta ttttaattcc aaaaatctgc tggtcaaaag accgagaaac
    32641 taaaaacatt actgaatgtg ttgataaatt atcttataga aataaactca aaatatatca
    32701 acacttgatt ctaaggagga tatgacaata tgaaaatttt agatagaatt aatgaacttg
    32761 caaataaaga aaaagtccaa ccacttactg tagctgaaaa acaagaacaa catgcattgc
    32821 gtcaagacta cttaagcatg atccgaggac aagtattaac aacattttcc acaataaaag
    32881 tggttgatcc aatcggtcag gatgtcacac cagacaaagt ttatgatctt cgccaacaat
    32941 acggttatat tcaaaattaa tatttgctca cgaggtattg cacttaaggt gccaactgac
    33001 ctcataaaca aagcccatac tgattgaaga cactaatgtg tccaccatgg tgcacattaa
    33061 accttcatct ttgtatgggc tttttattta ttcttttgag aatttcattt tagcagacca
    33121 aaaaattaaa atggtgtcca tcaagatctt tgaacattgc accataaaac ccttggctta
    33181 ctgttggttc ttgaacgact atgccacccg cttctttcac tttattcacc aactggtcaa
    33241 cttcttcatt tgtgttcaca cctagagaaa tcatcacttc atttggttca gtattaaggc
    33301 tttgctgact tacattttga aaatgcttgt tttctattaa aattacggtt gtttgaccta
    33361 tttgaatgcc gaccatttta tctaacattt gtgggtttct atttatttta aatcctaacg
    33421 ctttataaaa ctgtgcgctc ttttctaaat cttgcacatg caaattaaac cacattgatt
    33481 gaatcatgat tgcaccccat tcattactta ttatagtttt ggactttaag ccaatcactt
    33541 aatgataatc ttgttggatt tatttcagcc attaattcaa agtctacttc ataacctttt
    33601 tcttccaacc attgcttttc tgcaacacca ctaacaaatt ctccttctat aacagtagat
    33661 ttacctgtca cttcactaaa aattgttgct gcttcactta atgtaacttc atcggaacca
    33721 atctctattg attgatgcgt aaagctttgt ggatgtgcaa aaatatacga tgcaatttta
    33781 gctatatcaa tagaagaaat cattgtgaat tttatattcg gattaataaa ttctggtaat
    33841 gtaatacgtt catcttcgac tttagcaatg cgtaaaaaat tatccataaa gaatgatggt
    33901 ttgataactg ttgcatttat attagattcc attaatctat tttctatttt tgctagtact
    33961 tcaaagtgtg ggccagttcg atttcgatta acccctcccg cagtactata cacaatatgt
    34021 tgaatatttt cttgctcagc tatttcaatt atcttcatac cttgtcttaa ttcttcgcta
    34081 acatcatctt taacgattgg ctgaatactg tataagccat acttaccttt catcgctgat
    34141 tgcaaactaa cattatcact cagatcacct tcaacgattg ataaatgcgg atgtcctatg
    34201 tctgaaagtt tacgattatt cttatttcta gttaatgcac ttacatacca tccatcctct
    34261 aacaactgtt ttacaactgc attaccttgc ttccctgttg cgcctattac taaaatatct
    34321 ttcattttag atccactcct ctttttatct taaaaaccaa tagtcctctt tttcaccttt
    34381 taaatacgta tatggttctg gtgtttgtcc atttccttta atattgtcgt ttattacatc
    34441 tctgacatcc catactaaat gaataaatcg ttgtaattga tgactaaaaa tgcctagtgg
    34501 cgctgtcata gctaatactt tttccacatc ttctgtagtg acattcagct tttcagcaat
    34561 catttgaacc gataaagttg atgcttcaaa atgtgcttgc aattcttcag ctgtgatgcg
    34621 tctttgctct ttatttaaag acattatcaa cactcttttc ttttatgata tttaatagtt
    34681 attgcaaatt cattgctaac tttcttaaca cgttttataa taaacggaga gttatccgtt
    34741 tgtcaatttt agagaatttt aagtaggtgt aatcatgcga aaagatgcaa aagagaatag
    34801 gcaacgaatt gaagaaatag cgcacaaact ttttgatgaa gaaggcgttg aaaacattag
    34861 tatgaatcgt attgctaaag aactaggtat cggtatgggg acattgtatc gacatttcaa
    34921 agataaaagc gatttatgct actacgtcat acaaagagac cttgatattt ttatcactca
    34981 ttttaagcaa attaaagatg actatcattc taattatgag gttatgaaag tatctttaga
    35041 ttatctttta caatttaaaa tcgataataa ggcactactg caatgtattg aagcaggcaa
    35101 caataaatta cgtttttacc aaagcgcatt ttatcaagaa ttatttgatt tttattatga
    35161 cttatttaaa agcgatgacg acatttacaa gaagttcaaa acagatatgc tgttgcaagc
    35221 attatcaact agtgtatttg ccttccaaat agagcatcgc catatttcaa ttgaagcgta
    35281 cagaaattat ttattaaaca tttatttaga tgaggtggaa cgtaatgact aaaattgtgt
    35341 taatcacagg aggcaataaa gggttaggct atgcaagtgc agaggcactt aaagcattgg
    35401 gttacaaagt ttatataggt tctcggaatg atgtaagagg acaacaagca tcacaaaaat
    35461 taggtgttca ttatgtacag ttagatgtta caagtgacta ctcagtcaaa aatgcttata
    35521 acatgattgc tgaaaaagaa ggccgtcttg atatcctcat taacaatgca ggtatatctg
    35581 gtcaattttc agcaccttcc aaattgacac ctcgtgacgt tgaagaagta tatcaaacga
    35641 atgtattcgg tatcgtacga atgatgaata cattcgtccc tctcttagaa aaatctgaac
    35701 aacctgttgt cgtcaacgta tcaagtggtt taggttcatt tggaatggtt acaaaccccg
    35761 aaacagctga atctaaagtg aattcattag cttattgttc gtctaaatcg gcagtaacaa
    35821 tgctaacgct acaatacgct aaaggattac ctaacatgca aatcaatgct gctgatccag
    35881 gcgctacaaa tactgattta gttgatgatt ttagtaataa ttccaaacac gtttctgaag
    35941 gaatcaagcc aatcattcaa ttagcaacga ttggtgcaga tggcccaaca ggtacattta
    36001 ttaatggcga tggagagatg ccttggtaga tcaattttat gttacaaaca ttgaaattag
    36061 aggtatataa aaaatgcttc tgcaatagat gcaataaaca tgcaatacaa tatggaggcg
    36121 aagtaaatga aaagtattac gtttgaagaa cattatgtca ttgaagatat tcaaaaagaa
    36181 acgatgaatg cgatatcagc agatcctaaa ggtgtaccga tgaaagtaat gttagaaggc
    36241 cttgaaaaaa aagacaggtt ttacaaatgc cgacgaatta tcacatcatg atgaacgtat
    36301 ccaatttatg aataatcaag acgttcaaat tcaagtctta tcttatggaa atggttctcc
    36361 ttcaaatttg gttggtcaga aagccattga attatgtcaa aaagcaaatg atcaattggc
    36421 aaactatatt gcacaatatc ccaatcgctt tgtaggcttt gcaactttac ctatcaatga
    36481 gcctgaagcc gcagcgcgag aattcgaacg ttgcatcaat gatttaggat tcaaaggtgc
    36541 gctcattatg ggacgtgcac aagatggttt tcttgatcaa gacaaatatg acattatttt
    36601 caaaacagct gaaaatttag acgtaccgat ttatctacat cccgcgccag ttaacagtga
    36661 catttatcaa tcatactata aaggaaatta tcctgaagta actgcggcaa catttgcttg
    36721 ttttggttat ggttggcaca ttgatgtcgg tattcatgca atacatctag tattatctgg
    36781 tatttttgat cgttatccaa agttaaatat gattattgga cattggggtg agtttatccc
    36841 attcttctta gaacgtatgg atgaagcttt attcgctgaa catttgaacc accctgtaag
    36901 ctattacttt aaaaataatt tttatatcac accgagtgtc atgttaacga agccacagtt
    36961 tgatttagtc aagaaagaag taggtattga tagaattctt tatgctgctg attatccata
    37021 tattgagcct gaaaaattag gtgtattttt agatgaactg ggtttaacag atgaagaaaa
    37081 agagaaaata agttatacaa atggtgctaa attattaggt ttatcagcta acaattaaca
    37141 aatggaggtc attgaatatg gaaactttag aattacaagg cgctaaatta cgataccacc
    37201 aagtcggaca aggacccgtg ctcatcttta ttcctggtgc aaacggtacc ggagacattt
    37261 ttctgcctct tgcagaacag ttaaaagacc attttactgt tgtagccgtt gatcgtcgtg
    37321 attatggaga aagcgagtta actgaaccac tccctgattc cgcttcaaac cctgacagtg
    37381 attatcgtgt caaacgcgac gcacaggaca ttgccgaact tgcaaagtca ttaagtgatg
    37441 aacctgtcta tatattaggt tcaagttcag gttcaatcgt tgcgatgcat gtgttaaaag
    37501 attaccctga agtcgttaaa aagattgcat ttcatgaacc accaattaat acatttttac
    37561 cagacagtac ttattggaaa gataaaaatg atgacattgt gcatcaaatt ttaacagaag
    37621 gcttagaaaa aggcatgaaa acatttggtg aaacattaaa tatcgcacca attgatgcaa
    37681 aaatgatgtc tcaacctgct gatactgaag aaggacgtat agaacagtac aaacgtacga
    37741 tgttctggtc agaatttgaa attagacaat atacgcattc agatattact ttggatgatt
    37801 tcacaaaata cagcgataaa atcaccctac taaatgggac agattctaga ggctccttcc
    37861 cacaagatgt taacttttat ataaacaaag aaactagcat acctattgta gatattccag
    37921 gcggtcactt aggttatatt caaaaacctg aaggctttgc tgatgtatta ttaaatatgt
    37981 ggggttaatt gatcgttctc taataaaaca gcttggacag ttttcaaatt atatttgatg
    38041 gctgaccaag cttattttta tttacgctta aacgttcaaa tcaactcgtt attatacatg
    38101 aaggcatgtt aaccattctc ttccactgaa aacactactg ttccaaacat tgatagtctt
    38161 cttttgaaat accctttccg ataagcacca aatattctgg cacttttttt gaaaatgcta
    38221 caggtgttaa ttcatattgt ccttgtgtaa attgaatgag atacgtatgt gcggtgtctt
    38281 caaatgtcat aaaccctttc aacctataaa tatgagacgg aaggcatgct aaacgtttta
    38341 taaactgtgc tttagtacat ttggctggtg attgcacaaa ttgatgattt attgttttgt
    38401 gtaatgattc atgtttattt tcagaagttg ctgtcatttg gctaactgat attggcaaag
    38461 tgacgctgcc atgcatacca acttgtatat cggcctctgg gttaataact tctaaatctt
    38521 tcaatagttt gctcgtcgtt tcaacatctg ctgaatctat tttattaaca aataagacag
    38581 aacaatatgc taattgctca taaaataagc cttggatgtc tttagggaat gatttaatgt
    38641 gtttatacat gcttgcgtct attacaccaa tcatatgtgt aatggttgta aacggagcta
    38701 aaataggcgt taaacaagca tctaagacag agactggttc tgcaatccca ctacattcaa
    38761 taaatacaat gtctggttgc tcttttaaat ataattgatg taactgttct gatacatctg
    38821 ctttcattgc gcaacagata cacccctctg tcaattcact taaaggtatg tcttcagata
    38881 ctaaggcacc atcaacactc attttcccaa attcattcat gatgactgct ggtttcagcg
    38941 attcctttaa tgattcagcg agcaaatgat ttaagacagt cgttttaccg ccacctaaaa
    39001 acccacctat aataactatt ttcatctgcg actctcctct tatacttcat atgaatagca
    39061 tattccaaga tagatctcct attgactgac atgactctat attcagttat agttcatttt
    39121 tttaaataat cagcattact attttaacaa tgatgcctac accaataact aaagacaatg
    39181 atgtcaccat ttgctttcca attaatttta gtgctgactg accacctaaa tgtttcaaaa
    39241 gcatcgtcat tgtgaccgag cacgctgtaa atgttgaact aaaaaacaca agtagtagta
    39301 actgtgttgc tgtcattcct tgtaataagg cgccctgatg caaattaaac aagagcatgc
    39361 cgtcttttcg aatcattgaa aataaaatcc ctggtgacaa ttctgacgag atgcctaata
    39421 acgataatat aggtgtaaat atttgtgata aaacattcaa aattggcgtt agtgataaaa
    39481 tactaacaat aagacagatt gtgataaaaa taggtagcgc ttgaacgata aacatttgta
    39541 tattttgcca catttgcagc aacatttgac gtatatttgg catatgtaat tgtctatcat
    39601 aaggtagcgg aacgctaagt tgttgatcat tctttttcaa ccatatccta ttatgtaaga
    39661 tgccacctaa aagtactaat attaagtacg gcataaatag ccatgacttt ccagctacac
    39721 taaaaataga taatgtcgca cctatttgat aactacaaga actaccaaag cttattaaac
    39781 tcatacactg tgtcttcgtg caggtatggc attggtgtgc tgcttgtgaa atagctgctg
    39841 cattacatcc aaatccttct aatagtggca cgatatcatt accttgtaat ccgatccata
    39901 acattgacgg ttcaattgcc catatcatcc atgatttgag tcctgtttga tcaattattg
    39961 cagttgataa actaattaat attacaaccg gcaatgccca tacgagcgaa tatgttccaa
    40021 gtgatagcac accataatca ccaaataata tatgctgtaa tatatcgtga ttgagcaatg
    40081 ttgtttgagt taaatattct atccatgctg atacatactg attttgaaac caatctgaaa
    40141 attgataagc gataccgatt ggtaatgcaa acattaaaaa tatcaacaac caggctaaaa
    40201 atgtcgcact tattttaggc cttaaatgtc gtcgctcatg ttgaaatagt gtcaattgtt
    40261 ctatcacatg ttcgctcgct ataggcgaag aaactacact ttctgccaat gaaatatcta
    40321 acttctgttt tatgattaat aatcttaatt tcgctttttc tgttgcggta agtttatccg
    40381 catgactaat aactaaataa tgaggttcat taggtatggg cgtataatgc ccagttctca
    40441 tatcatattt cacttctaac atgtgcatga cacttcacaa catgaataat catctaaata
    40501 catttgattt ttttgataat tttcaatgac atcttgttta gctggtaagc cttcccgctg
    40561 tgttaaaaga tgggcaagta ctgcaaatcg cgctctgaat ttatagatat agcataattt
    40621 ggcattatca ttttcaactg ttgcaccaat cataaaaata ttcggataac gtgtcgattc
    40681 atcatgtgtt gttaatttaa tatcttgatt tgttgtcaca aataattgtt gaacgattgg
    40741 attttttgtt gcatcaaagc cagttgctag tattggttca tgaggcgtat gcacactttg
    40801 tccgctatca aaactgatat gatactgtcc attgttaaaa tcaatatctt taactgtata
    40861 atgtacattc atttcgatgc gagcaccttg cttaatgaca ttacctagtc gctgacgtgt
    40921 ataaggtgac aatctaacac taggatcagc atccggatca tttaaaccgg ttgtgctagt
    40981 ataaagtgcg atgtcagagc cattttttgc aagttgatat gcagcatcaa agccactttc
    41041 attacctccg ataaccacat attgcccctt attaaagtta tcaaagtctt caatttcact
    41101 ataatgaata ccatatttaa atggcttttt agggaaatta taatcacctg ttgcgacaaa
    41161 gatataatcc gcgtgatata tctctgttgt cgttgcaatc ctataatatg catcatctac
    41221 agatatattt gtgacaactg tattttcaaa gatattcagc tcgtaatggt tggcaaccac
    41281 ttgtaaatat tcagcatatg tttctccgga aatatgttct tcattaaatg taaatgctgg
    41341 tgaagtatcc atggaaattg cattcatatc aggcatgcca aatccattag acgtaaatga
    41401 tggcgtgatc gtacgggtcg atttcggcca atgtttaaat gaatgtccta ctgttccttt
    41461 ttctaaaata atgacatctg ttataccgaa atcttttaag gtaatggcca tacctatacc
    41521 tgcagcaccg gcaccgataa tagccacttt atgatgttgc atattaacac tccctcaatg
    41581 atctatttca agttattgtt tttgactata aatcgtaata attacgattt ataaagtata
    41641 gacctatcct tgagaaattt caatattaaa agaaaaactt ccgttattgt agagaattca
    41701 tctacatttc ggaagttggt atgtgtcttt tttattaaat ttaaagatgc tacggttagt
    41761 tcaaatgatt atgaatgaca tttcatggta tcatccagcc tcagtacttg gagataacat
    41821 tttggcggtg agacagaaat catattttta caaaatttat tggggtgtaa cttctccacg
    41881 ttgtatttca ctatccaaat tttgtttcat attttcaaac aactcttgaa atctaacctc
    41941 atcttgatca gaataacctt taaaaataac tgaatctata ttttcctcgt taaaataata
    42001 aatttcatct tctacaacac caatcggata tttacatgca gaatagtcaa acatatactt
    42061 ttgattttca atttctaaaa ttggtcctct attaataatc attaactttt gcgaaccttc
    42121 ttttaaataa ataatgcttc ctattgtttc catttatttt cctcctttgt catttacgat
    42181 attttcgcag tgtaattcat ttttaataaa caagatcaac ttttggattt gaatcttttc
    42241 attactttaa ttcgaaataa tattagttaa atcaattctt tattttaata tgaatttttt
    42301 tatccgaaat ggaggtcata tttaaacaca taaatataaa gaacataagt atccaaatga
    42361 tatacgttag aacattttgc ttattgtaaa aaatattacc tacgaataaa cctattacca
    42421 acattatatt aacaatataa atacaaataa ctagtaagaa attcttcaca gttgggatta
    42481 atataacgct tccaaaataa tgatgattat caatgtttat ttttaacttt ttagataaat
    42541 taatttttaa taaaatagat gctaacatag ctataataaa caatatcaac aatagtgtag
    42601 tactaatact tatatttact gcatctacaa aaccttttaa gaatatgcct atagttacac
    42661 caatgcctcc tagaaccata gacttattat tctgattacc attt
//
LOCUS       DAHHYJ010000006        42607 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000006 DAHHYJ010000000
VERSION     DAHHYJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 42607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42607
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.006"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     misc_binding    184..553
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            619..1722
                     /locus_tag="NBK22_000255"
     CDS             619..1722
                     /locus_tag="NBK22_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD2413472.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYNIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKGESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIERDIIQALDKAQIGEIV"
     gene            1719..2879
                     /locus_tag="NBK22_000256"
     CDS             1719..2879
                     /locus_tag="NBK22_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCD2413473.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKLEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISD
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTTAHLEQAKNGGAVIGFRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     AALPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            2848..4689
                     /locus_tag="NBK22_000257"
     CDS             2848..4689
                     /locus_tag="NBK22_000257"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCD2413474.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAATKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNIISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAYLSCYPNASLLDIENSEFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPKKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            4686..6914
                     /gene="metE"
                     /locus_tag="NBK22_000258"
     CDS             4686..6914
                     /gene="metE"
                     /locus_tag="NBK22_000258"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCD2413475.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEKAGVAKKLVIQTYFERAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            6957..7709
                     /locus_tag="NBK22_000259"
     CDS             6957..7709
                     /locus_tag="NBK22_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCD2413476.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSAEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIETFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(7792..8973)
                     /locus_tag="NBK22_000260"
     CDS             complement(7792..8973)
                     /locus_tag="NBK22_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCD2413477.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGELIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            9512..10645
                     /locus_tag="NBK22_000261"
     CDS             9512..10645
                     /locus_tag="NBK22_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCD2413478.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGLQYLLHIRTGLDYGDKRTCQAFASVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISSSLGII
                     VVSYIISLIFKGHDQVMIISVSVATFCIMLIYFKNQRLRNKNE"
     gene            complement(11015..11644)
                     /locus_tag="NBK22_000262"
     CDS             complement(11015..11644)
                     /locus_tag="NBK22_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCD2413479.1"
                     /translation="MKGMFLSSFYATRKQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRNDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMICASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIAYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(11644..12486)
                     /locus_tag="NBK22_000263"
     CDS             complement(11644..12486)
                     /locus_tag="NBK22_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2413480.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFGEEHTVDDVEYKEHIGVVFDDLRVPNKLTIKDIDKVF
                     QSIYMTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEHLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTGDKDVEIPKHLIIASRLSKGKYQILVKDYAEIKNAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(12511..13224)
                     /locus_tag="NBK22_000264"
     CDS             complement(12511..13224)
                     /locus_tag="NBK22_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCD2413481.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATYNNVV
                     VISIVATIIIILVEAIVLMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     ILQTVIAFLVLLIFVVGHAEANGILFFLIPFFVSAIFNTQFTLFNRRFDDRIPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGINQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(13221..13424)
                     /locus_tag="NBK22_000265"
     CDS             complement(13221..13424)
                     /locus_tag="NBK22_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2413482.1"
                     /translation="MRNRLKELRARDGLNQTQLAKLAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNETVETVFIIEEDEA"
     gene            13665..14054
                     /locus_tag="NBK22_000266"
     CDS             13665..14054
                     /locus_tag="NBK22_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCD2413483.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQNGTA
                     TYVHQSEDNIVTSSVKSTIPVAQKSNKTIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLQDQAVISELIPQ"
     gene            14153..14377
                     /locus_tag="NBK22_000267"
     CDS             14153..14377
                     /locus_tag="NBK22_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCD2413484.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            14433..15095
                     /gene="tatC"
                     /locus_tag="NBK22_000268"
     CDS             14433..15095
                     /gene="tatC"
                     /locus_tag="NBK22_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCD2413485.1"
                     /translation="MLLSFVITVIIVYVSSFWWMTLVISYITRAHVSLHAFSFTEMIQ
                     IYVMIIFFIAFCLISPVMFYQLWAFVAPGLHNNERQFIYKYSFFSVLLFCAGVAFAFY
                     VGFPMIIQFALKLSLTLNISPVIGFKAYLVELIRWLFTFGILFQLPILFIGLAKFGLI
                     DTTSLKHYRKYIYFACFVLASIIAPPDLTLNILLTLPLILLFEFSMFIVKFTCRGKPP
                     TH"
     gene            complement(15187..16899)
                     /locus_tag="NBK22_000269"
     CDS             complement(15187..16899)
                     /locus_tag="NBK22_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HCD2413486.1"
                     /translation="MKHYLTKFVAMLITAAMVCSFGLLKSQAAEQQSISDVYSVITDA
                     KSALSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDDQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTAKVSHAWTTFKSNIDHIDK
                     KSDTSANDQYHVSQLNDELEKAIKAIDDNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKKVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFIIGIALAIVILIIFALLFRFIVKLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(16880..18109)
                     /gene="efeB"
                     /locus_tag="NBK22_000270"
     CDS             complement(16880..18109)
                     /gene="efeB"
                     /locus_tag="NBK22_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCD2413487.1"
                     /translation="MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKS
                     MFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMAD
                     RMMDGDTVGKTSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFK
                     DLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISA
                     KGKETPRNLMAFKDGTINPRKSNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTA
                     LEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILR
                     RAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFL
                     VLPGVSKGGYLGETLFD"
     gene            complement(18106..18960)
                     /locus_tag="NBK22_000271"
     CDS             complement(18106..18960)
                     /locus_tag="NBK22_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413488.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(19246..19773)
                     /locus_tag="NBK22_000272"
     CDS             complement(19246..19773)
                     /locus_tag="NBK22_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2413489.1"
                     /translation="MFGMKVNEQITLKILEAHDTEALFNLVNRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGNGFQCGIWYEGTLVGVIGLHEINHMHRKTSLGYYLDKQ
                     FECHGIMTQAVEALIKYCFEELDLNRIEISAAVNNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLK"
     gene            20192..21187
                     /locus_tag="NBK22_000273"
     CDS             20192..21187
                     /locus_tag="NBK22_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCD2413490.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTTVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(21247..21813)
                     /locus_tag="NBK22_000274"
     CDS             complement(21247..21813)
                     /locus_tag="NBK22_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD2413491.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRELE
                     LEFSVGKNYLDTTVDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFS
                     LKALAQSTMRTAKVQQQVFEEENNQYDF"
     gene            complement(21827..22888)
                     /locus_tag="NBK22_000275"
     CDS             complement(21827..22888)
                     /locus_tag="NBK22_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD2413492.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRA
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDVSSKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNEIIPAIKKHLSK"
     gene            complement(22902..23828)
                     /locus_tag="NBK22_000276"
     CDS             complement(22902..23828)
                     /locus_tag="NBK22_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2413493.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLDNNGI
                     TSERLYYLGQEGVVFKDEDDLEIILLVNDSFEVPHQWQHNVYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRANKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPLMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPVEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            24140..25498
                     /gene="glpT"
                     /locus_tag="NBK22_000277"
     CDS             24140..25498
                     /gene="glpT"
                     /locus_tag="NBK22_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCD2413494.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(25581..26021)
                     /locus_tag="NBK22_000278"
     CDS             complement(25581..26021)
                     /locus_tag="NBK22_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCD2413495.1"
                     /translation="MCKSKILLKNIFSEESEVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKSGYFVTCWTKDEDNYNRPYKIEEFADYLIVAVIDDELNGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFFEY"
     gene            complement(26125..27480)
                     /gene="mepA"
                     /locus_tag="NBK22_000279"
     CDS             complement(26125..27480)
                     /gene="mepA"
                     /locus_tag="NBK22_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCD2413496.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYFIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMVSMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIIYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKAVIMSIGVIFVVCMSAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMASLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(27587..28006)
                     /gene="mepR"
                     /locus_tag="NBK22_000280"
     CDS             complement(27587..28006)
                     /gene="mepR"
                     /locus_tag="NBK22_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCD2413497.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            28218..30173
                     /locus_tag="NBK22_000281"
     CDS             28218..30173
                     /locus_tag="NBK22_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD2413498.1"
                     /translation="MLSYRQIEILYLLIHEQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDHYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEIDKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSN
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            30178..30621
                     /locus_tag="NBK22_000282"
     CDS             30178..30621
                     /locus_tag="NBK22_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2413499.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHAAFCDEDHYASLIFVL
                     SAIDNHSHLSVLQNLATVLGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            30623..30907
                     /locus_tag="NBK22_000283"
     CDS             30623..30907
                     /locus_tag="NBK22_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2413500.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            30922..32268
                     /locus_tag="NBK22_000284"
     CDS             30922..32268
                     /locus_tag="NBK22_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD2413501.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYIHESISDSQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGVLITFLPLLFLPFL
                     GELGGAATTFSDTDFLTVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(32361..33386)
                     /locus_tag="NBK22_000285"
     CDS             complement(32361..33386)
                     /locus_tag="NBK22_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCD2413502.1"
                     /translation="MNNKVLVTGGTGFVGMRIISRLLEQGYVVQTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSK
                     LLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVST
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAIDS
                     MAKYHLI"
     gene            complement(33702..34724)
                     /locus_tag="NBK22_000286"
     CDS             complement(33702..34724)
                     /locus_tag="NBK22_000286"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD2413503.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQHIFLDEDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGATDVVQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRDITIEEFKNLMVTQILGID
                     DIKEAKRYELSDADWEAIEELASKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIYGDFFGQGDIKDVEEALQGTKMTREDLTHQLKQLDIVYYFGNVTVESL
                     VEMILS"
     gene            complement(34702..35646)
                     /locus_tag="NBK22_000287"
     CDS             complement(34702..35646)
                     /locus_tag="NBK22_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCD2413504.1"
                     /translation="MQSSKWNAMSLLMDEKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLMEGKHYHIITTNADNAFDAAEYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLRRYNA
                     FLEQHQDDKVLYLEIGIGYTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTEDISTLITVALRNDSTTKNNNIGETEDVLNRTD"
     gene            complement(35636..36436)
                     /locus_tag="NBK22_000288"
     CDS             complement(35636..36436)
                     /locus_tag="NBK22_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCD2413505.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNHQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNQDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEALNRDAE"
     gene            complement(36437..36769)
                     /locus_tag="NBK22_000289"
     CDS             complement(36437..36769)
                     /locus_tag="NBK22_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCD2413506.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            complement(36803..37804)
                     /locus_tag="NBK22_000290"
     CDS             complement(36803..37804)
                     /locus_tag="NBK22_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD2413507.1"
                     /translation="MVKLSVLDYALIDEGKDAEKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEQVKAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            complement(37918..38010)
                     /locus_tag="NBK22_000291"
     CDS             complement(37918..38010)
                     /locus_tag="NBK22_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413508.1"
                     /translation="MENIFKIELMNGICRSENMNMKKKNKGEKI"
     gene            38106..39305
                     /locus_tag="NBK22_000292"
     CDS             38106..39305
                     /locus_tag="NBK22_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD2413509.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRTRLCLEVMRAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGEHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            39378..40205
                     /locus_tag="NBK22_000293"
     CDS             39378..40205
                     /locus_tag="NBK22_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2413510.1"
                     /translation="MTEIKDKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARNRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTDSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTFERVNKNEHDFDRRPNDEAITIYRKLVDFLNAITMM"
     gene            complement(40446..42518)
                     /gene="lip2"
                     /locus_tag="NBK22_000294"
     CDS             complement(40446..42518)
                     /gene="lip2"
                     /locus_tag="NBK22_000294"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCD2413511.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTN
                     AAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQ
                     NSKTIRTQNDNQVKQDSERQGSKQSHQNNATNKTERQNDQVQNTHHAERNGSQSTTSQ
                     SNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTH
                     QPAHQITDAKQDDTVRQSEQKPQVGDLSKHIDGQNFPEKPTDKNTDNKQLIKDALQAP
                     KTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKF
                     KVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKT
                     YKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGG
                     HNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFK
                     QLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSS
                     HTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTN
                     DEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATES
                     KGTQLKAS"
BASE COUNT        13450 a         8251 c         6572 g        14334 t
ORIGIN      
        1 ctaaatgagt cagacaacca ttaatcacac tttatttaaa aaataattca tttttcaact
       61 tcaagatacc aattacaatt tcacaaacaa attaactttt aaatcaaaat tttctgaata
      121 attgttgaca aatctttttt tactctgtaa gctaattaat atcttataac agtttaatga
      181 aacgtaaaca caataaagag gaaagtaaaa cacaccctgc ttatacagag agtctttagt
      241 agctgagaga agattttgaa agcgtgtttg aaaatggcct tggagtgttg atgccaatat
      301 gaggtgtcta cgggttcgcc cgttatagcg atacagtatt aacattgatg ttaaatggcg
      361 tactggattc tttacgcacg attttttgtt aataagtatg ggatagcaca ttactatatc
      421 cttacttact gactttaatt gtgataattg ttcagtaagc atatttactt ttaatgcgta
      481 ctgaataagg ttatttcagc gatggaataa caaataaagg tggtaccgcg aaacataagc
      541 tttcgtcctt tttatccgat tcattcgggt acgaaggacg gaagcttttt ttattttttc
      601 ttataggagg gtcttaatat gaaggataca cagttagccc aaatcacatt aaccgatgat
      661 tcaaccggtg ctatagcgaa tccaatccat ttatctactg cctacaagca tccaaaacta
      721 ggacaatcga caggttttga ttatacacgt actaaaaatc ctacacgctc aacatttgaa
      781 acctgttttg ccaaactcga acatggtatt gcatcattcg ctacatcaag tggaatgtca
      841 gccattcaat taatatgtaa tttatttaaa ccacacgatg aaattttagt ttcattcgat
      901 ttatacggcg gcacatttag attatttgaa ttttatgagc aacaatacaa tatcaaattt
      961 aagtacgttg attttacaga ttatgaacaa gttgaaaaag aaatcactga taaaacagtt
     1021 gcattattca ttgaaccaat atctaaccca caaatgattg ctattgatgt aaagccgtac
     1081 tatcaacttt gtaaagctaa aggcttattg tcaattatcg acaatacatt tttaacacct
     1141 tatctttcaa caccattagc agaaggtgct gatatagtct tacattcagc tacgaaatat
     1201 attggtggac ataacgatgt actagcaggt gtcgtaaccg tcaaaggtga atcactcgcg
     1261 caacagttgt ttgattttca caacatgact ggcgcaacac tttcaccaat agatagttat
     1321 ttgttgttac gtggacttaa aactttgcat ttacgcattg agcgtgcgca atcaaacgct
     1381 agaaaacttg ctaaaaaatg tcagtcactt caagcaattg acgaagtact atatagcggg
     1441 caaactggca tgcttagttt aagacttaac aaggcctata gcgtcgctaa attattagaa
     1501 aatttagaca tttgcatttt tgcagaaagt ttaggaggta ctgaaacatt agtgaccttc
     1561 ccttacaccc aaacacatgt tgatatgcca gatgctgaaa aagataaacg tggcattgat
     1621 gagtatttaa tccgcttatc actaggtgtt gaaaattatg aagacatcga acgcgatatc
     1681 atccaagcat tagataaagc tcagattgga gagattgtat gacactttca aaagagacag
     1741 aagtgatatt cgattggcgt agaggtgtgg aatatcattc agctaaccca ccactctatg
     1801 attcttcaac attccatcaa acaagtcttg gtggcgatgt taaatatgat tatgcacgaa
     1861 gtggcaaccc taaccgtgaa cttttagaag agaagttagc acgattagaa cagggaaaat
     1921 tcgcttttgc ttttgcatca ggtattgctg ctatttcagc agtacttttg actttcaaat
     1981 ctggtgatca tgtcatctta cccgatgatg tatatggcgg tacttttcgc ctcactgagc
     2041 aaattttgaa tcgatttaat attgaattta caaccgtaga tactacaaag ctcgaacaaa
     2101 tcgagggtgc cattcaatca aacacaaaat taatttatat cgaaacacca tcgaatccct
     2161 gttttaaaat tactgatatc aaagctgttt ctaaaatagc cgaaaagcat gaactactgg
     2221 tagctgtgga caatacattt atgacaccgt taggtcaatc acctttatta cttggcgctg
     2281 atattgtcat tcatagtgct acaaaatttt taagtggaca tagcgattta attgctggtg
     2341 ctgtcataac taataatgag gcaattagtg acgctcttta tttaatacaa aatggtacag
     2401 gcaatatgtt atctgctcag gatagctgga cacttgctaa acatttaaaa acttttccaa
     2461 tcagatttaa acaatctgtc gaaaacgctc aaaaaatcgt gtcattttta ataaagcaag
     2521 atgaaatttc agaagtttat tatccgggac tcactactgc tcatttagaa caagctaaaa
     2581 atggcggtgc cgttattggc tttcgtttag ctgatgagtc taaagcacaa caatttgtcg
     2641 atgcactgac attaccactc gtttcagtga gtctgggcgg tgttgaaacg atcctttcac
     2701 atccagcaac aatgtctcac gctgcactac ctgaagaagt gagacaagaa cgtggtatca
     2761 ctttcggtct attccgatta agtgttggtc tcgaagatcc tgatgaactc attgcagaca
     2821 tcaaatacgc attaaaggag gcattcaatg agtcaattcc tcacacaatt gaaagataat
     2881 gttttagtag ctgatggcgc tattggaacc attttatact ctgaaggatt agacacctgt
     2941 ccagaagcat ataatcttag ccatccagat aaagttgaac gtatccatcg ttcatatatc
     3001 gaagctggtg ctgatgtcat tcaaaccaat acttatggtg caaattttga aaagttaaaa
     3061 cgattcggtc ttgaagataa agttaaagct atacatcaag ccgccgtacg catcgcaaaa
     3121 aaagcagcaa ctaaagatac gtatatatta ggcacagttg gtgggtttag aggtatcaaa
     3181 caagaggata tcagcttaca aactattctt tatcatactg aaattcaaat agacacctta
     3241 attgaagaag gcgttgacgc actacttttc gaaacgtatt acgacctaga agagctaaca
     3301 aatatcattt cacgaacgag aaagaaatac gacattccaa tcattgctca attaaccgct
     3361 tcaaacacaa attacttagt taatggtcag gcaatcaatg aaggattaaa acaactcgtt
     3421 caatgtggtg caaacatcgt gggactcaat tgtcatcatg gcccgcacca tatgcaagag
     3481 tctttcacac atattgaatt accagagcac gcatacttat cttgttatcc aaatgccagc
     3541 ttattagata ttgaaaatag tgaatttaag tatagtgaca atgcacaata tttcggtcaa
     3601 gttgctcaaa atctaattcg cgaaggtgtt cgtttaattg gtggttgctg tggtacaacg
     3661 cctgagcaca tcaaatttat taaagaatct attcagacac ttaaacctgt taatgacaaa
     3721 aaagtgattc cgataccaaa gaaagcactt ttcaatccat ctcaaaataa agttagacaa
     3781 tcattaacat ctaaggttca agaacgtcca accgttatta tcgaattgga tacaccgaaa
     3841 catttagaca cggatagatt ttttgaaaat atcgctaaac ttgataaagc taatgtagat
     3901 gcggtaacac tcgcagataa ttcattggca actgtcagaa ttagcaacat tgctgctgca
     3961 agcttaatta aacaatatta caatattgaa ccactcgtac atattacttg tcgagaccga
     4021 aacttaatcg gcttgcagtc ccatttactt ggattatcgc tcattggcgt taacgaaata
     4081 ttagccataa ctggtgatcc ttcaaaagtt ggtcacttac caggtgcaac caatgtctat
     4141 gatgttaatt ctaaaggatt aactgaactc gctctaagat ttaatcaagg tattaacact
     4201 gacggtgatg cgctgaagaa acgtacacac ttcaacatcg ctggcgcctt taatcctaat
     4261 gttcgaaaat tagatggtgc cgtcaaaaga ttagagaaaa agatagaaag cggaatgtct
     4321 tattttataa cacaacccgt gtacagcaaa gagaaaatca ttgaaattta ccatgccact
     4381 aagcacttga acaaaccatt tttcataggc attatgccta tcgcaagtta caaaaacgca
     4441 ctctttttgc ataatgaagt gccaggaatc aagatgtcag atgaaatttt acaacaattt
     4501 gaagcagtta aagatgataa agccaaaaca cgagaactaa gtcttaagct ttcaaaggat
     4561 ttaatcgata ctgttcatga atattttaat ggtttataca ttatcacacc gtttcaaaat
     4621 gtcgaagatt cattagaact tgcagcatac tcaaaatcta ttactgctca caaggaggca
     4681 atattatgac aacaattaaa acatcaaact taggattccc aagattaggt agaaaaagag
     4741 aatggaaaaa agccatcgaa agttattggg caaagaagat ttctaaagaa gaattagatc
     4801 aaacattaac tgatctacat aaagaaaatt tattattaca aaagtactat cacttagata
     4861 gcatcccagt tggtgatttc tctttatatg accatatatt agatacgtca ttattattca
     4921 acatcatccc tgaacgtttc caaggaagaa ctattgatga tgatttatta tttgatattg
     4981 ctcgcggtaa taaagaccat gttgcaagtg cacttataaa atggtttaac actaactatc
     5041 attacatcgt acctgaatgg gacaatgttg agcctaaagt gagtcgcaat gtattattag
     5101 atcgctttaa atatgcccaa tctttaaacg ttaacgctca ccctgtcatt gttggtccga
     5161 ttacttttgt taaattatca aaaggcggtc accaaacttt tgaagaaaaa gttaaaacgt
     5221 tattaccact ttataaagaa gtgtttgaat cattaattga tgcaggtgct gagtacattc
     5281 aagttgatga gccaatctta gttacagacg acagcgaaag ctatgaaaat attacacgtg
     5341 aagcttatga ctatttcgaa aaagctggtg ttgctaaaaa attagtcatt caaacatact
     5401 ttgaacgtgc acatcttaaa ttcttaagtt ctttacctgt tggtggttta ggtttagact
     5461 ttgtccatga taatggctat aaccttaaac aaattgaagc aggagatttt gataaatcaa
     5521 aaacattata cgctggaatt attgatggtc gtaatgtatg ggcaagtgat attgaagcta
     5581 aaaaagtctt aatcgacaaa ttgttagcac acactaatga gcttgtcatt caaccatcat
     5641 cttcattatt acatgttcca gtatctttag atgatgaaac attagataca agtgttggcg
     5701 aaggcttaag ctttgcaact gaaaaattag acgaattaga tgcattgcgt cgcctattta
     5761 atcaaaacga tagtgttaaa tatgataaat taaaagcacg ttatgagcgt ttccaaaatc
     5821 aatcattcaa aaacttagat tatgatttcg aaagcgtgcg tacttcaaga caatcaccat
     5881 tcgcgcaacg tattgaacaa caacaaaaac gtttgaactt accggattta ccaacaacaa
     5941 ctattggatc attcccacaa agccgagaag ttcgaaaata ccgtgcagat tggaagaaca
     6001 aacgcattac agacgaagca tatgaaacat tcttaaaaaa tgaaattgct cgatggatta
     6061 aaattcaaga agacatcggc ttagatgtat tagttcacgg tgaatttgaa cgtaatgaca
     6121 tggttgaatt cttcggagaa aaattacaag gtttcttagt aactaaattc ggttgggtgc
     6181 aatcatatgg ttcacgcgcc gtaaagccac caatcattta tggtgatgta aaatggacag
     6241 cgcctttaac tgttgatgaa acagtttatg cacaaagctt aacagataaa ccagttaaag
     6301 gtatgttaac tggacctgta acaattctaa actggtcatt tgaacgtgtt gatttaccac
     6361 gtaaagtcgt tcaagatcaa attgctttag caatcaacga agaagtatta gcacttgaag
     6421 ctgcaggaat caaagttatc caagttgacg aacctgcatt acgtgaaggc ttaccattac
     6481 gctctgaata tcacgaacaa tatcttaaag atgctgtttt atcatttaaa cttgcaacgt
     6541 cttcagttcg tgatgaaact caaatccata cacatatgtg ttattctcaa ttcggtcaaa
     6601 tcattcatgc tattcatgac ttagatgctg atgttatttc aattgaaaca tctcgtagcc
     6661 atggtgattt aattaaagac tttgaagata ttaattatga tttaggtatt ggtctaggtg
     6721 tatatgatat tcatagtcca cgtattccaa caaaagaaga aattactaca gcaatcaatc
     6781 gttcactaca acaaatcgac cgctcattat tctgggtaaa ccctgactgt ggtttgaaaa
     6841 cgcgaaaaga agaagaagtt aaagatgcat tgactgtgct tgtgaatgct gttaaagcta
     6901 aacgccaaga ataatcgcag catttatcat ataataaact aaaagcgggt gattttatga
     6961 cacaatatcc tttatggcaa caattaaata ctttaaaaca agcaacttgg gttgatttaa
     7021 cgcatacgtt cgatccggaa ataccacgtt ttagtgagtt tgaaaaaggc gaagtttcaa
     7081 ctttattcac ggttaaagat catggtttct atgtgcaacg ttggaatatt gtaacgcaat
     7141 atggtactca cattgatgca ccaattcact ttgtcgagaa taaacgttat ttagaagaca
     7201 ttgatttaaa agaattggta ttgccgttaa ttgtattaga cttttcagca gaagtagcga
     7261 acaacaatga tttcatcgta acacgtgcgc atatagaagc atgggaaaaa gagcatggta
     7321 ctattgaacc tggcactttt gttgcacttc gtaccgattg gtcaaaacgt tggcctaata
     7381 ttgaaacgtt tgaaaacaaa gatgcgaacg gacaacaaca tgcaccgggt tggggattag
     7441 atgctttgaa gtacttaatt gaagaacgtc gtgttgaagc tgttggtcat gaaacattcg
     7501 ataccgatgc atctgtggac gttgtaaaaa atggtgattt agttggcgaa cgttatattc
     7561 ttggtcagga taaataccaa gtcgagttat taactaattt agatcaatta ccaactcgtg
     7621 gcgctatcat ttacgctatc agccctaaac caaaagatgc acctgggttc cctgttagag
     7681 catttgcaat taaaccatca aatgactaat attacccatt aaatccataa acaaataaaa
     7741 acaggttatg ccaacatgta attaatactg ttgtgataac ctgttttctt attatttata
     7801 ctttgataca actgcagcga tagcttgacc gccaccaata cacaatgatg ttaaaccagt
     7861 ttcaacttca tcattcagtt gatgcaataa tgtcactaaa actctagcac cagatgcacc
     7921 aatagggtgt cctaatgcaa tagcgccacc tttaacattc accttttcag gaggtaattt
     7981 taattcacga tcaacagcta atgattgtgc tgcaaatgct tcatttaatt caaatacatc
     8041 aatatcgctt aattcttttt tactacgttt caaagccttt tctacagcgc caactggtgc
     8101 aatacccata atagaaggat ctacgccatg acttccaaag ccatcaagca ctgccaatgg
     8161 ttcgatattt aattctttag ctttgtcttc tgacatgact aacatcatcg cagcaccatc
     8221 attgattcct gatgcattac ctgctgtaac tgtaccgtct tttttgaaag ctggtcttaa
     8281 gcgacttaat ttttcgactg atacattttc acgtacacct tcatccttag tgactacgat
     8341 tagttcacct ttacgttgag gaatcgatac tggaactatt tcactatcaa attcaccatt
     8401 ttgctgtgca cgtactgctt tttgttgtga gtttacagca aatgtatctt gttcttctct
     8461 tgaaatacca tattgctcta ctaaattttc agcagtaata cccatatgat attgattaaa
     8521 tacatctgtt aaaccatcat ataccatgct atcaaccatt gattgatgtc ccattttaaa
     8581 accgaagcga ctgttgttga caagcattgg tgactgagac atattctcca taccgccagc
     8641 tagcacgatg tcattttcac cagtcacaat agattgatat gctaattgaa tcgactttaa
     8701 cccagaacca catactttat tcactgtaaa tgcaggtact gtttctggca agccaccttt
     8761 catagcagca attcgtgctg gattttgtcc ttgtcctgct tgtagtacgt taccgatgat
     8821 aacttcatca atctcacttg gattcaaacc cgtctcttta ataatatgtt ctattaaagt
     8881 cgcacctaaa tcataggctg gcacgtcttt aaacgcacct ccaaaaacgc caataggtgt
     8941 cctgtatgct gctgctaata cgactctcgt catgtcatat tcatctcctt atgatatata
     9001 caattttatt actaatacgt ctaatgaacc aatacgccac taacatccaa tttatgaaac
     9061 caattgctac gtatgttaca ttttcattct tgttatgcat ttaacaaatc atattataca
     9121 atgatgtaat ttgtttgggc aatcggaaac gtaaacggtt tcactttaac atttcacact
     9181 cttaaatatg ctctatcatc ctcatctata gtacaaaatt atgctttgat ttaccacaaa
     9241 ttatcattaa tcatttcgcg taatgactct aattaaatgc tatctatcat tgcatcgcta
     9301 ttcacagctg gtttcacaat caatttctca tcaatttttt attaccatga cgtctccagt
     9361 caaatattaa atcgctgatg attgttaatt ccgcaattac cttgcagtca cctctccttc
     9421 cacttcatcg ccaaatctgc ccctattcac tagaaaaaat aaatagtata cttagtaaat
     9481 taattacaaa tcttaaagga gttaaaattt tatggttaat gcattaacac caaaacattt
     9541 agctgaaaag cgtgcaggat tccatgagtt gttctttgat ttaattttcg tctatgccat
     9601 tcaaaaaatt gcacatgtga ttttaacaac acaaaatggt tccatttcag ctgatttatt
     9661 tttcaaatac atcgtcatga gtttgttctt atggttaatg tggtctcatc aaacattttt
     9721 tacaaatcga tttggtcaag ttacattcaa agacgtctcg tttatgatgt ttaatatgtt
     9781 catcatggtc ttcctatcta acagcctata tccggacttt gaaaagacat tcttcccatt
     9841 cttcttatgc gtggcaatta tgtatttaag tatcggatta cagtatttat tacatatacg
     9901 tacagggtta gattatggcg ataagcgtac atgtcaagct tttgcgagcg tagcctttgt
     9961 gatttcattt ttatcatttc tatcattagt actaccacaa agtatccatt acatccctgg
    10021 atttttaggc gtattcattg cagcgacagg acttattcca tttcagaaat atcttgtatt
    10081 atcacctgtt aatatgatgc atttagtaga acgtttttcg ttactaacaa ttattatctt
    10141 tggcgaggta ctggttggtt tagcttcgtc atcatttagc attgatcatt ttagttacat
    10201 ttatatattc caatttatga ttttaattag tcttttcggt gtatattgga tcattactga
    10261 aaattacatt aatcataaat tgtcatctat cggtttccgt ttatcgtata cgcatctatt
    10321 aatcaatatc gctttaggtg tgattaatgc tgcaattgtt tttagtaata acaataaact
    10381 aaatgacttg tttgaaatta atatgatgta tatcagtgtt ttaatattct atattggatt
    10441 atggttaatc acgccttatt tccataatga actgacaaac gcaaaatata tttcttcttc
    10501 actaggcata attgtagtat catatatcat cagccttatc tttaaggggc atgaccaagt
    10561 aatgattatc tcggtatccg ttgcgacttt ctgcatcatg ctgatttatt ttaaaaatca
    10621 acgtctacgt aataaaaatg aataaatagt attcacctcg gtcaaagtat ttgaactgaa
    10681 tccaaaagtg tcgaacttca cattatactt ttgtgacaag cttcagttat gctacatgat
    10741 cgaggttttt aaatatttcc aaggaaatcg ttagcctata caatcatcaa atgatactca
    10801 taaataacat atattctcta atacccacta tctaaaagga aataattccc acacaacata
    10861 accacctcaa aacaaatgaa tcaatctaga tatgtcacct atacttatca ccctgcattt
    10921 cattccacac caaaaagagc agatgataat cgtattgatt tatcacctgc tctaaatatt
    10981 atttcaagga ctagcttcac accaagaata ttgattatag ttcttgtttg ttaaaaattt
    11041 taatagacaa tatataagaa acgctcgtta taactatacc aatgaccgca tatgctatga
    11101 caactagcca tccttcagtg ctaatttttt caaattctaa tgcagatgca ccactaacca
    11161 taccatatat aaagaaaaag accacgaaag aaataagtag tagtatagat gcacatatca
    11221 taatgacatt agagttttca gcgccaaatt taaatgtcaa tgggaaaatg atagagtatg
    11281 cccctatgat accaaaactc attaaacctg acataatacc aatcatcaca ctttgggtca
    11341 cgattgtagt cacaactaat ccaatcatta aacttgcacc gaataagatt aaataaaagg
    11401 caaagtatga cttaatataa tcattacgtt taaccggtaa agtagataca taatacatcc
    11461 atcttgagtc tttttcatgt ttaatattat cagtaatggg tgtgattaac ataaccccag
    11521 ccattgccga actcatcaac ggattaaata ctgcaaagta tcccgcagct atgatagcga
    11581 ctataaaata aatatatgtt tgctttctcg ttgcataaaa actacttagg aacatacctt
    11641 tcattatact tcacctcgca ttatgatttt cgtagcgtca tcaatgtgtt ttaaaggttc
    11701 tgcattttta atttctgcat aatctttaac taaaatttga tatttcccct ttgacaatct
    11761 agaagcaatg attaaatgct taggaatttc aacatcttta tctcctgtcg taacaattcc
    11821 atattgtgct aaaagtatat ctttctgttc agttaaaatc attcgtccat ctttcataaa
    11881 cactaattta tcagctaaat gttctatatc ttcagaaata tgcgatgata ttaagatgcc
    11941 tccaccttga gcgacaaaat cttctaatag ttccattact tcttctcgtc cagaaacatc
    12001 catacctgca gttgcttcat ctaagattaa taacttcaca tcatgagaaa gcgcaattgt
    12061 taaagctatc ttcattcgca tccctcttga aaaagtttta attttagttt gtagtggtaa
    12121 ctcgaaatat ttgattaaat caaagaattt ttgactattc caagtcatat aaatagattg
    12181 aaatacttta tcaatatctt taatagtcaa tttattaggc actctcaaat catcaaacac
    12241 tacacctatg tgttctttat attcgacatc atccacggta tgttcttctc caaaaaatgt
    12301 gatactaccg ttatctttat gtcgattgcc tactagaata ttaattaatg tcgatttacc
    12361 tgagccattt ttcccaatta atccaactgt ctcattagta gaaatcgtta aagatgcatc
    12421 ttgcaattta aaatccgaat ctttgtattg tttgttcaca ctatctacgt ttaacaatgt
    12481 tgtcatatcc atgctcctct ttgtttaatt ttaataaaaa cgtcttctct tcaataaata
    12541 actaaaggcg ttatatatga aaatagcaat gataagtagc aaactaaagc tttgattaat
    12601 tccagtagta attgaataaa gcccaattac tacaatggct agtatcaata ttgataagtt
    12661 gatcgcatat gttttaaata atgcaattaa ttctatatgg cgttcacctt catccaatat
    12721 ttccaatcgc ttttcagtgt aattcttatc tgcaattttt ggtattctgt catcaaacct
    12781 tctattaaac agtgtaaatt gtgtattgaa aatagcacta acaaaaaatg gtattaaaaa
    12841 gaatagtatt ccatttgctt cagcgtgccc taccacaaaa ataagcaata ctaaaaaagc
    12901 aataactgtc tgcagaatac ttaatatact tccatttaaa acataacgat tcgcaagcaa
    12961 ttcatattga tctgttgcat cgatatctac ctcttcatct acaagtcgct tatacttcaa
    13021 tgcacgtctt tgattcatca aaacaatggc ttctaccaat atgataataa tagtcgcaac
    13081 aatcgatatt acaacgacat tgttatatgt cgcaaacgtc aagttctcaa tacccaatcc
    13141 atcaaagtta cctaaaattc cacctatgat accacctaca agtccaccca gtaaaagata
    13201 tccgatatat cttagtattt tcatgcctca tcctcctcaa taataaaaac cgtttccacc
    13261 gtttcattga aaatgcgagc aatctttatt gccgttaata ctgatggcat aaaattgttt
    13321 cgctcaatta gcgaaatggt ttgtcttgaa acaccagcta gtttggcaag ctgcgtttga
    13381 tttaagccat ctcgtgctcg taactctttc aatcgattac gcacatcgca tcaactcctt
    13441 agtcctactg taaatgatat ttgtcttttt gacaactata tttgtcaaat ctacacaaaa
    13501 aaatatgatt gtccacctat atatgacatt ttgaaacaaa cacctcaacg cctacaagtc
    13561 ataattgttt actttcgtta caccttcctg cataattaac agcattctaa ttttagtatg
    13621 atgcacgcat tttcactaaa tcaaatcatt caaaggagat tattatggca tttacattat
    13681 ctgcaattca acaagcacat caacaattta ctggtgttga ttttccaaaa ctattcaaag
    13741 catttaagga tatgggtatg acgtacaata ttgttaatat tcaaaatggt acagcaacat
    13801 acgtacatca atcagaagat aatatcgtta cgtcatctgt gaaaagtacg attcctgttg
    13861 ctcaaaaatc aaacaaaaca atagttcaag acgtcttaac aagacatcaa caagggcaaa
    13921 cagattttga aacattttgt gatgaaatgg ctgaagctgg catttataaa tggcatatcg
    13981 atattcaagc gggcacttgt acttatatcg acttgcaaga ccaagctgtt atttcagaat
    14041 taatccctca ataaactata tttatagcaa cattttaatt attttataaa atttattgat
    14101 aatcattatc gttcggtata aagtaaatac tatatactac ttatgagtga ggttgattat
    14161 catgataact aacactttta ttttaggcat cacaggccca acaagtcttg tcgtcattag
    14221 cattatcgct ttaattattt ttggtccgaa aaaattacca caatttggtc gtgctatcgg
    14281 ttctacttta aaagaattta aatctgcaac agaagattta gacaaagagt ctcacgacac
    14341 acccagtaag gaatcgaaac aacagcgaga gcaatagcac tgaccacacc ttactagttc
    14401 actttagcga actacgccat cggttagtaa aaattttatt gtcgttcgtc attacggtca
    14461 tcatcgttta tgtttcatca ttttggtgga tgacattagt catatcatat attactcgag
    14521 cacatgtgtc cttacatgca ttttcattca cagaaatgat ccaaatatac gtgatgatta
    14581 tatttttcat cgcgttttgt ctcatttcac cggttatgtt ttatcaattg tgggcgtttg
    14641 ttgcgcctgg tttgcataat aatgaacgtc aatttattta taaatatagt tttttcagcg
    14701 tgttattatt ctgtgctggc gtcgcttttg cattttatgt tggctttcca atgatcattc
    14761 aattcgcgtt aaaattatca ttaactttaa acatttcgcc tgtcattggc tttaaagcgt
    14821 atttagttga acttatccgt tggcttttca cttttggcat tttattccaa ttgccaatat
    14881 tattcatagg acttgcaaaa ttcggtctta tagataccac atcattgaag cattatcgaa
    14941 aatatattta tttcgcttgc tttgtattag caagtatcat tgcaccaccc gatttaacat
    15001 taaatatatt actcacattg ccactcatat tgctctttga gttcagtatg ttcattgtta
    15061 agttcacatg ccgaggcaaa ccgccaacac attaacctta taagaattaa tgctgtgatt
    15121 atcgttatac tgcaataccg aatatttcca acatcgcaac aaaaaaacag gcactcggcc
    15181 tgttttttat tttttaaatt taaagattaa gatagcaact accataatat aagcagcttg
    15241 tgctatcaat ggttcataac ttggatagaa gcccaaccag ttaatcgttg ggaatccttc
    15301 aataacatgt cttggcatcg cacctaataa ttgtaacttt tgaatactta cgccaagcat
    15361 tttgaatccc ataataaaaa taaagatcga caacactcta aatatataga aaataggtat
    15421 taatttaact ataaatctaa ataataatgc aaagatgatt aaaataacga tagctaaagc
    15481 aataccaata ataaaatctt tggtcgctag ctcacctatc atccccatat agaaaatgat
    15541 aacctcgaca ccttcacgca acacagatat taaaccaatc gtcgctaaca ataccaaatt
    15601 accattacta atcgcattag catacatatt tttaatcatg tcattccaac gttttgcatt
    15661 tgaacgtttg tgcatccaaa caccaacgat aaacattaat atgaccgcaa cgatacctaa
    15721 tcccgcttcc atactttcac gaagaatgcc actattccct aaagtttcta caaacgtaat
    15781 tgctaagata atactcagta caagtccggc aattgcacca ccaatcacac ttgcagtacc
    15841 cttcttatct ttgacattac gcgtcatagt agtcaatgtc attacaatta ataacacttc
    15901 tagcccttca cgtaaaaaga taatcatcac atcgacgaag ctataactat ggccaacaac
    15961 ctttttaatt tggttattta aatctactaa accatctttc acatgtgctt tattatgttc
    16021 gtctaataca ctttgataat atggtatttt atcttcaatt ttcgtataca aagcaccgtc
    16081 tttagtttga atttgacctt caacatacgg ccaaatttct ataaaatgtg taagcgcagc
    16141 atcagcatcc gacaattgat tgtcgtcgat agctttaatc gccttctcta actcatcatt
    16201 taattgtgat acatggtatt gatcatttgc agacgtatca ctttttttat cgatatgatc
    16261 aatatttgat ttaaaagttg tccaagcatg tgacactttt gccgtatcta atggtgattt
    16321 atgaattgca attctaagtt gtaataatgc gacttcaatt tgtccatatt gatttgcgtc
    16381 ataattgcga atcactgttt cattacttgt ccaaatctga ttcaaactat tgttcaaaga
    16441 ttctaattcc gctttatttt tatctttaat cgcttttgtc atcgcagcat ctttggcatc
    16501 gacttgctgt tgtaatagtt taattttaga acccgcatct ttactagcca atttctcttc
    16561 ataagcaatt aatgacttcg ttaattgcga aagtgtatct ttttgatcat cattcgcttt
    16621 tgcatcttca agctttctca catctgattt gacagcatta ccttcactat tatcttcaag
    16681 cgataatttc ttaactgcac ttaccacttg ctcaattgct ttctgcttat tgtcattcga
    16741 tatcgaatta ttagaaagtg cagatttcgc atccgttatc acactatata catcactaat
    16801 actttgttgt tctgctgcct gacttttcag taacccaaag ctacacacca tagcagcagt
    16861 tattagcatt gctacaaatt tagtcaaata atgtttcacc aaggtatcct cccttactaa
    16921 cacctggtaa tactaaaaat gaagcagaac ctctatgtgt aatatattca tttaatttat
    16981 cattactacc taaattattt tgtatatcga taaattgttt tgtcgctttt tgaaaagcaa
    17041 tgaacaacaa gcctgtttcg aagttacctg tgcggtcatc cgtgccatca acataattaa
    17101 aggctctacg taaaattgac gtatttgctt ctttcgctag tctcgtatgg gcatctttat
    17161 caataatata ctcgccatga ctatctttcg cttttaagtc aatttcatca aactctttcc
    17221 cgcctgttaa cggcgcacca ctatgtcgtt tccgaccaaa tgtagcctct tgttcttcca
    17281 gtgcagtacg atcccacgtt tcaatgtgta tttgaatacg tctgacaaca caataagttc
    17341 catgtttcgc ccatccgtca tcaataaaca cataatcttt aagttgatta ctctttctag
    17401 gattaattgt tccatcttta aatgccatta aatttctagg tgtttcctta cctttagcag
    17461 agataaaacc agattgcgcc caacgtacct taacaatatc tcgaaatgga cgaactaaat
    17521 tatgaaccgc atgaaaggat acttgcgaat catttgagca tgcttgaatc ataatatcac
    17581 catcgctgta atcgtctatt aactgatcat tcggaaaatg cggtaaatct ttaaaggcat
    17641 cgggaatttt actagataac ccaattttct tcatcaaaga cttactaatc ccaaaggtaa
    17701 tcgttaactt gcttgcacct aatcctatcg attcaccggt atctactggt ggcattaaag
    17761 gattgttact cgtcttgcca actgtatcac catccatcat acgatcagcc ataaccgtcc
    17821 actttttaaa cattgcctta attgcatctc tatcttttga cttcaaatct aacgcaacga
    17881 aattgcatgt tttttgcgtg ggtgtggtaa tgcctggttg cactttacca taaaattcat
    17941 acgcatcttt ttccggatct tctggtgtat tgaacattga tttgaaagac cacatgctac
    18001 caacaccact tgcgccaatt gcaacaccgg caccgccaat acctaacatt ttcaaaaatg
    18061 tacgtcttga aaattgcgta ctatcgttaa cttgttcata atttgtcatt cagtaatcac
    18121 agccatttta cttagtggct ctcctaatgc attaacagca tccgcaaatg ctttacggtc
    18181 tttcttagat actttttcaa atgactcata accgccgttg ttatctttat atttatccaa
    18241 taattgattc actttatcga agttcatttg aatatcatca cttaattttt tatctttttt
    18301 ctctaaaata ggtttaaata agtcataaat tttttgtgca ccttcaacgt tcgctttaaa
    18361 atcatataaa tctgtatgtg aataaatttc ttcttcacct gtgattttag aagttgcaac
    18421 ttcatttaat aggtcaacag aaccttgtaa cattaatttt ggtgtgatat ctaatgtatc
    18481 agctttggca tgcaattctt ttgcatcttt caataattgt tgtgcatctt ttttagtcac
    18541 atcatcaatt ttcttatctt cgtataatgc tttttcaatc ttatgatatc ctgaccattc
    18601 tttttctttt ttctcttctt tcatatctgc aagacgtgca tcaattttag gatctaaatc
    18661 tccaaatgct tctgcaactg gttcagagcg ttcataatac atacgaactt ttggatataa
    18721 cgcttttgct tgggccatat cattattttc aatcgcttta acaaattttt ctgtaccttt
    18781 taaaaattca tttaactgtt catcagtata tttatcatat gcttttgttg cttgttttaa
    18841 ttctgcttta acaccatcat cttttttcga tgtctttgaa tcatccttct tactatcgtc
    18901 gttcccacat gcagcaatta ataacgttga tgctaataat agcgttgtta actttttcat
    18961 tttgacacct cattatagaa attctattat ccccatagat gtgttcttat aaatattatt
    19021 ttcgcattcc ctacacgcta aaataataca ttacacttaa aacggctgtt ttaaagcatc
    19081 ctcccataaa catcatctag ttgataataa ttatcaaaat cattatattg ttataaaata
    19141 atgctgtcaa caacctttta ttttcgaaaa ttaaataaaa gagcgatgcc attttaaatg
    19201 tcatcactct tttgtaagtc taatttgtca tttttggtcg tattcttatt ttaataaact
    19261 atagatgtaa ctcgatgaat aaataccatt tagtaattca ttgtcacgta acataccttc
    19321 tctagtaaat cccagccttt caggaatagc tcggcttttt tcattattaa ctgcggcact
    19381 aatctcaatt cggtttaagt caagctcttc gaaacaatac tttatcaatg cctcaactgc
    19441 ttgtgtcata atcccatgac actcaaattg tttatctaaa taatatccta atgaagtttt
    19501 tctgtgcatg tgattaattt catgtaaacc gatgacacca actaacgttc cttcatacca
    19561 aatgccacac tgaaatccat taccatcagc aaattgcaaa agtcctcttt taataaatgc
    19621 acgcgtatct gatggttgct cagttgcatc tacccaaggt aaccattccc taagtgaatt
    19681 tcttgaacga ttgactaaat tgaaaagcgc ttctgtgtca tgagcttcta aaatttttaa
    19741 tgttatttgt tcattcactt tcattccaaa cataccatca catcctcatt catttttcat
    19801 ataattctgt aaattatcat aataataaca tattaatatt acgccttgta tcttaaattt
    19861 gttgtccttc cccaacttgc attgcttgta gaatttcttt tcgaaattct ctatgttggg
    19921 gccccgccaa cttgcattgt ctgtagaaat tacggaccca atttctctat gttggggccc
    19981 atccccaact tgcacattat tgtaagctga cttttcgtca gcttctttgt tgggaccccg
    20041 ccaataagcg ttgtagtgcc tattactttg gttgatgtcc ttgataccta tcacacaact
    20101 taatttaatc actactttat tattcattcc aaatttatat ttaaaaatac gacaaaacag
    20161 tgctaaaatg ctattaaagg aggcactgct catggcatca ttgaaaaaca agcattttat
    20221 gattggacta tcattaacat ttatagtcgc attatttagc tttttagcag ctaaattgcc
    20281 aatcttagat aaggttggcg cattaactat cgcgatatta atcgctattc tatatcgtca
    20341 ctttagagga tatcctgagc aatatagctc aggtattacg ttttcatcta aatatttatt
    20401 aagatttgca atcatcctat atggacttaa actcaatata ttcgatatta tcggtcaagg
    20461 tagtagacta ttagccatcg atgtaggtgt cgttattttt agtattgtaa tgatgctctt
    20521 tgtcaacaaa ctgttacatg gtgacaaaaa catcgcatta ttactaggtg taggtacagg
    20581 cgtttgtggt gctgccgcta ttgctgcagt agctccaata ttcaaatcac gtgaaaagga
    20641 tacagctatt agtatcggta tcatcgcatt gattggtacg atattttcac ttatatatac
    20701 agctatctat gctatctttt caatgacgac aactgtctat ggcgcttggt ccggcgttag
    20761 tcttcatgaa attgcgcatg ttgtcttagc tggcggcttt ggtggtagtg atgcacttaa
    20821 aattgcactg ctgggcaaac ttggcagagt attcttactg attccattaa ccatcgtact
    20881 tattttaatt atgcgtttcc gttcatcaga atcatctagc aaaggtcgaa taagcattcc
    20941 atacttttta attggatttg tcattatggc attagtgaat acatatgtaa ctattccgtc
    21001 agcattgcta aatattttaa atactgtatc aacaatttgc ttattaatgg ccatggttgc
    21061 tttagggtta aatgtggcat ttaaagatct taaaaatcgc gcacttaaac cgcttatgac
    21121 aatcattatt acctcaatat gcttgtcatc acttgccttt atcgtcgttc attggctata
    21181 tagttaatat gaaaagacgc tcaaacactt acaaatttag tgtgccgagc gtcttatttt
    21241 tatactttaa aagtcgtatt ggttgttttc ttcttcaaac acttgttgtt gtactttggc
    21301 agttcgcatt gtggattgtg ccaacgcttt taaactaaat acaacatcat cattgacaat
    21361 ttgttgattt gaaaaatctt tctcttcaat aaatacatac gtttgcatcg tatgtgcttt
    21421 catgtaactc aatattggtt ttaaatgcat ttcaggaatt aaataatgtt tactagaacc
    21481 tgctgtcgct acaagtccta ttaccttgtc acgaaacgca ttgaccggaa gcaaatcaaa
    21541 tacatttttc aaagcaccag ggatggaagc ttgaaaaatt ggaaaaccaa taaaaatcac
    21601 atcagcctgc attaacgacg tcgttaattt atatacatct actgtagtat ctagataatt
    21661 ctttccaacg ctaaattcta attcaagttc tcgtaaatcc atcaactcta tttgatgtcc
    21721 ctcattgatg acttctagtt cattttttaa atcatccata gcaattctcg ttttagaacc
    21781 tactgtggaa cctgacaata atacaatatt catgatgacg cccctcctat tttgataaat
    21841 gctttttaat cgccggtata atttcgttgc caattaactc aatattcttc ataacatttt
    21901 caaatggcac accgccaaaa tcaagctgtg ccataaaacg ttgatgacca tacaactcgt
    21961 gttgatacaa tatcttttca ataatttgtt gcggacttcc aaccattagc gcttctcgat
    22021 aatctgacga attagcaaat tgctgttttg gataaccaac gccacgaata aatgacatcc
    22081 ctgtattcaa atgtggataa aattctctca tagcatcctg agttgtttca gctgtataaa
    22141 acagactcgc tgtacttact ggtaaagact tagacgaaac atcgaaacct gcttcagttg
    22201 ctgcttgacg ataagcatct atagaacctt taaagttcat tgctgggcca ccaagggttg
    22261 taatcatcat tggcacacct tgttttcccg ctttgatagc acttgcaggt ggaccaccaa
    22321 cagcacgcca tattggcaat gtattatcga ttggtcttgg gaatattttc atatttctaa
    22381 gttctggacg atattttcca gaccaagtaa tacgctcagt tttatttaac tctaaaagta
    22441 aacctaattt ttcttcaaac aattcatcat agtcttttaa atcatagcca aacaagtcaa
    22501 aaatacctgt tcttgatgct ctaccagcca caatttcggc tctaccatga gaaatcaaat
    22561 ctaatgtcgc gaagtcttca aatgctctta caggatctgt tgcactaata atcgttgaag
    22621 aacttgaaac tttaatatgc tttgtcgctt gggcaattgc acctaacaca accgtatgtg
    22681 cctgtgttgt aaaatgctcc tgatgacttt caccaactgc aaaaacatca atacctgctt
    22741 catctgctaa tttacttgct tcaataattt cattaatacg ttgctcataa ctaacttttt
    22801 cacctttcaa tggattcaat aaatgatcac ctaaggaata caaaccaaac tcaagaggcg
    22861 tatttttatt catttctaat ttggccatta aacattctcc tttatatttc gtgtaactta
    22921 ctttcaattt gttcacgttc tgcttctaaa aagtcaggca agttcaattg acttcctaat
    22981 tgttcaacag gtgtatcaat agtaaatcca ggcgcttcag tcgcaaattc atacataatt
    23041 gaattatggc gatagtataa tgatttaaag aaatagcgat caattatacc tgaattactt
    23101 tgtggttgtt gttgtaattt cttgtaaatt gcatctaaat cactcattag tggtgtatta
    23161 actgcaatat gatggatata acctcgtcca ggtctttcac gttgtccctg ttgctcaata
    23221 actacaaaat ccgaatataa accttgtggt gctaatgtca gcacatcgaa tgatttatta
    23281 gctcttttgc gataacctaa gacattttcc aaaaattcta ccgtacgcgc tgcatttcta
    23341 actcttaatt cgactggccc tattcctaaa atttgatatg cttgaggtat ttcactataa
    23401 acgttatgtt gccattgatg tggtacttca aaactatcat taacaagcaa tatgatttct
    23461 aagtcgtctt catctttaaa gacaacacct tcttgtccaa gatagtacaa acgttcagac
    23521 gtaataccat tattatcaag gcgtttttca aaataatgaa gtgccgcttg atttggtact
    23581 aataaagaaa atcgataaat cgtttcagtg cctggctgct tatgacccgc attgggaatt
    23641 tcaaaaaagc ttaaaattgt tccggctgta cctacttcgt ccccataaaa caaatgatac
    23701 attgaaggat tgtcttgatt aaccgacttt tcaactaatc gtaatccaag gacatttgtg
    23761 taaaaatcct tattacgttt tgcatctttt gtatacatgg atatgtgatg atgccctact
    23821 atattcatac tatccctcct attagtataa attttatacc agcttacata aaaaagatag
    23881 ttatatgcca tttacccctt ttctgcaaat aaaaatatgt ttgtactcat tcgtagatac
    23941 acagctagta tgtataacga cataataatt gcataattat aaatttatgt gtagcaattt
    24001 attttttcta aatgtttata aaatacttca ataactaaat atgcaagatt tcacaaaagt
    24061 atattgacaa cgctttcata catgtctaat ataagtttat cgcacaaaat gcatataaat
    24121 acatattaag gaggatttta tgaattttct taaacctgca aagcatatta agcctttgcc
    24181 agaaaatcag atagatgata cctataaacg attacgtctc caagtatttc ttggtatttt
    24241 catcggttac gctgggtact atttattacg taaaaacttt tcgttagcga tgccggcatt
    24301 gcaagagcaa ggttttacaa aagcggaact aggttttgca ctttctgctg tttccatcgc
    24361 atatggattt agtaagttct ttatgggtac tgtaagtgat cggagcaatg ctcggatatt
    24421 cttagttctt ggattagcac tcactgctat cgtcaatttg ttaatgggat ttgtaccgtt
    24481 ctttacatca ggtatcggta ttatgtttgt cctattattc ttaaatggat ggtttcaagg
    24541 tatgggctgg ccaccttcag gccgtgttct cgttcactgg tttagtgtaa gtgaacgcgg
    24601 aagtaagact gccctttgga acgttgcgca taatgttggt ggaggtatta tggcacctat
    24661 tgctgcttgg ggtattacaa caacagcatt tatcaacttt ggttatttaa aaggtttcga
    24721 aggtgtattc atttaccctg cactcttagc acttatcatt gcagcaattt catacgtatt
    24781 gattagagac acacctcaat ctcaaggttt acctccaatc gaaatttata aaaatgactt
    24841 tgctacaagc gataagaaaa cattagaaac agaattaact acaaaagaaa ttttatttaa
    24901 atatgtacta aacaataaat gggtatgggc aattgccttt gcaaatatat ttgtttattt
    24961 cgtgcgttat ggtgtacttg attgggcgcc agtctactta agtgaagaaa aacatttcga
    25021 cttaaaagca tcaggttggg catacttctt atacgaatgg gctggaattc ctggtacatt
    25081 attatgtggt tacatttctg ataaattatt caaaggtcgt cgaggacctg caggtttctt
    25141 ctttatgtta ggtgtcacag tatttgtatt aatttattgg ttaaatcctc caggcaatgc
    25201 ttggttagac aatgtctcat taattgccat tggtttctta atatatggac cagttatgtt
    25261 aattggttta caagcattag attatgtacc taaaaaagca gctggcacag cagctggatt
    25321 aacaggatta tttggttatc tgtttggtgc tgtaatggcc aacatcgtct taggtgctgt
    25381 agttgataaa ttcggatggg atgtcggttt tattttatta acagcaatca gtgtgtttgc
    25441 aatgttgagc tttatcctca cttggaataa agtaggacaa gaaaccgttc atcattaaat
    25501 gataaaaaat aaagtcatag ggttatctta tcgaaagatg atatattcat ctcttataag
    25561 ttcaaccata tgacttttta ttagtattca aaaaaatatt tacattgcca cttttgtgtt
    25621 tgccctgctg ttttattcaa ttgattacac cacttaggat aaactctaaa agccatctta
    25681 cctttatgtt caaatgtagt taagatacct ttttctacca ataattccct aggaaataga
    25741 aagtagccat ttaattcatc atcgataaca gcaacaatca ggtaatcagc aaactcttca
    25801 attttgtatg gccgattata attatcttcg tcttttgtcc aacatgtcac gaaatatccc
    25861 gatttagtcg gtgttttctt agctaaccta ctttgatatg tttcctcttt aaagctaaat
    25921 gttaatgctt cgtaatcttg attatatttt tcttcagtta aatctttaac ttctgattct
    25981 tcactaaaaa tatttttcaa cagtatttta gatttacaca tatgccaatc tcactttatt
    26041 ttttcataat cgtatcatat atttattttt ttcgaaaaat acacttccga ccgaaatata
    26101 ctcagccaga agtggacgaa atatttaacc ttctattaat tcagatgtat caactgtcaa
    26161 acgatcacgt aataaataga cgattaacat tgctgctaaa gcacaaagtg actcagcaat
    26221 taataatgac caaatgacac ctgttagtcc aaacaaagca ttcataataa ataatactgg
    26281 aatgataatt gcaccttgta aaatggccat aattgtagca ccacgacctt gcccagtcgc
    26341 ttgaagcata ccagtaaaca agaaacctat accatttaat aataatgatg ccattgttac
    26401 tttcaaaata aatgtcgcca tctcaacaat ggcttgatca gtagtaaata gtccgaccat
    26461 atgatgtcca attgtaaata cagcactcat acatacaaca aagataacgc cgatagacat
    26521 gataactgct ttgataacgt ctttcatacg gcctttattt gccataaagt tatatgcaat
    26581 tagtggtaca acaccttcac ataatcccat gataataagt tctggaaatt gcacaagtct
    26641 aaatgagata ccataacttg caatcgcgaa gtttccataa tgtgctaaaa ataaatttaa
    26701 aactaatcct gtgaatccca ttaagatact cattaaaaat gcaggaatac cgattttaaa
    26761 gatttcagaa agcatttctt tattaggttt cgcaagttta atattaactg acacaacgtc
    26821 actatttttc ataaaataaa tgataaagaa cagagcagca gcaacattac tgattgcagt
    26881 acccaaagct gcaccaacaa cgtttaaatc aaaaccaaaa attaaaattg gatctaaaat
    26941 aatatttaag cctacactag ctaacatacc aatcatagaa accattggtg ccccaattgc
    27001 acgtgcaaat tgttctaata tgaagaacaa aattacaaaa ggtgcactta aaaacattac
    27061 tttcaaataa ttacttgtta aagctaacgt ttcacctctc gcccctaaaa ttgctgcgat
    27121 ttgatcactg aatggtaaag taactaaaat cacgataagt cctagtgcaa taccaccata
    27181 aatagagaaa ctacttacaa atttactctt actatagtct ttcgcaccta ataaacgtga
    27241 aatataagtt cctgcaccaa cgccaaataa attacctaac cccattaaga tagcaaatac
    27301 tggcagtgtt agagagatag cagaaatcat gtggctatct tctaaaaatc ctataaagta
    27361 aatatttaat atgccataaa taacgcttaa taaagtccct atcatcattg gcaatgagaa
    27421 atgcatcatc gctttaaata ctggcgattt ctcaaaataa tataattgtt cgtctttcat
    27481 tgttcaatac tccttgtctt ttccaataat tagcttacta acaattagat atctaactat
    27541 aatattaaag acaaagtgac tgatttctac cagtcacact tatcatttat tgtaaactag
    27601 ataacatttt agttaagttt gctttcattt gttcattttc ttcttcagat aactgcgata
    27661 cgagtgtttg ttccatttca tcaaatatcg aagtgaatgc ttctacgagt ttaatcccag
    27721 aggtagtcag ccctatattc tttcttctcg tatcttgtgc atcgacatag cgatagatca
    27781 gctttttacg ttcaaggttc cttaataaat tactgacagt tggacctgtt cgttgtaatg
    27841 ctttagcaat atcattttgt gtcagtccat cttgttgatg tgcataaaga taacctaacg
    27901 tatgaccttg ctcatttgta atatcaaatt gctctaactt ttgatcagcc ttttgtttca
    27961 tctcatgact aatcattcta aataaatacg aataagtgaa ttccatttat tgcaccacca
    28021 tttcgttaga tgtctaacta aatatactgc gacattttta aattgtcaac atgaatatct
    28081 aatttatttt aaaagttcct ataaaatctt gcgccataaa cattttgata tgcaattttt
    28141 cggattaaaa tatgaaagag attgcatatc tacgttttta agaaatcgct tacaattaca
    28201 aataggagga tattacgttg ttaagttata gacaaatcga aattttgtat cttttaattc
    28261 atgagcaaac gtttattcca atcaatacca ttgctaatca acttggtgta tcacctcgta
    28321 ccattcaata tgatatagca tatattgagc aatatgctga aacttatcat taccaagtca
    28381 gccgtaataa agctgcaggc atcaaagtaa caacagctca cgcgacacta ttaaatgaat
    28441 tagagcacaa tctaacaaat caaattcatt tttcaaaaga tgaacgactc acacatatcg
    28501 ctttaaagtt attcgaaaca accgatcctg tttcaacaaa gcaacttgcg caagatgtta
    28561 atgtttcgcg tcggacaatt gcagatgata ttaaaatgat tcaagcccaa ctagatcaat
    28621 accatttaaa gttaaattat gtccacaata aaggattcaa tattattggt gaggaagatc
    28681 attatcgtaa agcgtatgca cactttatac atcaatatat gaaacaagct gcacctttta
    28741 tagaggcgga tatctttaat tcagaatcaa tcgcattggt tcgccgtgcc attattaaga
    28801 cattaaatag tgaaaattat catttagttc agtcggctat cgatggctta atctatcata
    28861 tactcattgc cattcagcgt ttaaatgaaa atttttcgtt cgatatacct atcaatgaaa
    28921 ttgataaatg gcgacatact aatcagtatg ctattgcttc aaaaatgata gaaaacttag
    28981 aacgcagttg taatgtcaca tttccagagt ctgaaattat ctttatcacg ttacatttac
    29041 taggctctaa aatgactgaa catacagcat cttcaattac ctttgaatac catgatttat
    29101 cgcaaaatat acatgaattg atcacttgtg ttagccaaga attaggcatt gatatgtcaa
    29161 aagacaacaa gttacatacc agtctgatca cacatatcaa accagctata catcgtatta
    29221 aatacgatat gctacaacct aatcctttga ggcaagaagt tatgcgtcgc tatcctcaaa
    29281 tcattgaagc cgttagcaag catattagtc caattgaaca agatgctgct attcgcttca
    29341 acgaagatga attaacatac attacaattc acttcgcatc aagtatagag cgtgttgcaa
    29401 cacataaaca atcaatgatt aaggttgtct tactatgtgg ttctggtata ggcacgtcac
    29461 aacttttaaa atcaaaacta aatcacctgt atcctgagtt tcacatttgg gatgcctatt
    29521 ccatttatca attggaagaa agtcgattat tgcaagataa cattgattat gtcatttcaa
    29581 cagtaccttg tgaaatatca gctgtaccag ttattcatgt cgatccattt atcaatcaac
    29641 aatctcgtca aaaattgaat caaattatca atgactcaag agaacaacga gtcatgaaaa
    29701 tggcaactga tggcaagtca ctcgcagatt tattgcctga acatcgcatc attataaata
    29761 aacaaccatt atcaattgaa tccgcaattg cagtggctgt gcaaccttta atcaatgatg
    29821 gcattgtcta ttcaaattat acagctgcaa ttttaaaaca atttgaacaa ttcgggtcat
    29881 atatggtcat tagtccacat attgcactta ttcacgctgg tactgattat gtacagaatg
    29941 gtgtaggttt cgcactaaca tatttcactg aagggattat ctttggtagt aaagctaacg
    30001 atcccgttca ccttgtaatt acattagcaa cggaccaccc caatgcacat ttaaaggcat
    30061 tgggacagtt aagcgaatgc ttaagcaacg acttatatcg acaagatttc ttagatggga
    30121 atatttttaa aattaaacaa cacattgctt taactatgac aaaggaggct taataacgtg
    30181 tcattagaca ttttgtcaac aacacgcatc attgtaaaag aacaagtaaa tgattggact
    30241 gaagctataa ctatagcttc tcagccatta ctacaagaac aaattattga acaaggctat
    30301 gttcaagcaa tgattgatag cgttaatgaa cttggacctt atatcgttat cgcacctgaa
    30361 attgcaattg cacatgcaag accgaacaat gacgtacatc aagttggttt aagtctatta
    30421 aagttgaatc aacatgcggc attttgtgat gaagatcact acgcatctct catttttgta
    30481 ttgagtgcca tcgacaatca ttcacactta tctgtattac aaaatttagc aaccgtactg
    30541 ggcgataacc aaacagtcca gcaactatta actgcaacaa atgcacaaga cattaaaaac
    30601 attttaaagg agcatgatta atatgaaaat tttagtagta tgtggccacg gtttaggaag
    30661 tagttttatg gtagaaatga acgcacaaga agcacttagg caacttaatg caccatctga
    30721 tatcgaagtt gaacatagtg acattatgac agcaagtcca gagatggctg acttgtttat
    30781 ttgtggtaga gatttagctg aaaatgccga acgtctaggg gatgtcttag ttcttgataa
    30841 tattttagat aaagctgaat tacaacaaaa gctctcagaa aaattacaac aacttaacat
    30901 gatttaaagg aggtacgacc tatgcaagca atccttaatt ttatagtcga tattttaagt
    30961 caaccagcca ttcttgttgc actgattgcc tttataggtt taatcgttca gaaaaaacct
    31021 gccgcaacga tcacttcagg aaccattaaa acgatattag gcttcttaat tttaagtgca
    31081 ggtgctgatg tcgtcgttcg atctcttgaa ccattcggca aaatattcca acacgcattt
    31141 ggtgtgcaag gtatcgtacc taacaacgaa gctatcgtct cactagcctt aaaagatttt
    31201 ggaacaacag ctgcactcat catggtctgt ggcatgattg ttaatatttt aattgcccgc
    31261 ttcactaatt taaaatatat ctttttaaca ggtcatcata cattttacat ggctgcgttt
    31321 ttagcaatca ttttaacagt cagtcatatt aaaggctggc taacgattgt tatcggcgca
    31381 ctcgtattag gattaatcat ggcagtatta cctgcattac tccaacctac gatgcgaaaa
    31441 attacaggga atgaccaagt agctttaggt cattttggct caatcagtta ctttgccgca
    31501 ggtgctgtag gtcaattatt caaaggtaag tctaaatcaa cggaagagat taaatttcca
    31561 aaaggcttaa gtttcttacg agaaagtaca attagtatct cgattacgat ggcattactt
    31621 tacttcatcg catgcttatt tgcgggcgtt agttatatac acgaatctat tagtgatagt
    31681 caaaacttta ttgtcttttc attaattcaa ggtgtgacat ttgctgctgg tgtatttatt
    31741 attttaacgg gcgttcgttt aatcttagct gaaatcgtcc cagcatttaa aggaatttct
    31801 gaaaagcttg taccaaattc taaacctgca ttagactgcc ctattgtgtt cccttatgca
    31861 caaaatgcag tattaattgg attctttgtc agctttatta caggtgtcat cggtatgttt
    31921 atcttattct tatttggtgg cgtcgtcatt ttacctggcg tagttgcaca cttcttctta
    31981 ggtgcaacgg ctgctgtatt cggtaatgca agaggcggta ttaaaggtgc tgttgctggc
    32041 gccgctctaa atggtgtcct aatcacgttt ttaccattat tattcttgcc atttttaggc
    32101 gaattaggtg gtgctgcaac aacattctca gatacagact ttttaactgt cggtatcgtg
    32161 ttcggtaacg cagtaaaata tatgggatta tttggtgcga ttctatttat tattatcgta
    32221 ggtgcgacag caattttatt aaaaggccgt caaaagccac agcaatagtg ttaacgtaga
    32281 aatataaaac accgtcacat attgagtgaa tgcccctttt atcaagagga aagccactta
    32341 cttatggacg gtgttttgta ttatattaaa tgatacttag ccatactatc aattgctgct
    32401 aaaattgctt cttcttgtgt cgcaatcggt tcccaaccaa gtaatgtttt tgcacgttcg
    32461 ttacttacat tgcggttcat atctaataaa agtttacctt ctttagcttg atgattaaat
    32521 ttagcaccta gactcaaaat aaagtctggt aattttttag tagaaacttt ttgagctatt
    32581 tcaggtctct tttctttaat taattttgca atttccaaca aattaatttg tccatcagcc
    32641 gtcgcaataa atcgcttgcc attagcttgt tcatttgtca ttgccaaaat gtgcagttca
    32701 gctacgtctc tcacatcaac aacatttaac ggaatttgag gtacacgttt cattgaacca
    32761 ttcaataaat tttctaataa atgaaagctt cctgaaacgt gtgcatctaa tgatggccca
    32821 aaaattgcaa ctggattgat tgtggcaaat tctactgttg tattttcatt ctcaacaaaa
    32881 tcccacgctg ccttttcagc taacaatttt gatttttcat atactgataa gcctggttca
    32941 tcttcatttg tccaatgact ttcatttgtg attgaatttt tatctttatt actaaaacca
    33001 actgcaccaa agtttgcagt cattaccaca cgtttaacac ccgcatgttc tgcagctctt
    33061 aaaatacgtt gtataccttc aattgcaggc ttcgccatca cttctgcatc gtctgtttta
    33121 ccgaaaaaca ccggagatgc tacactcaag acatacttgc aatctttcat tgcttcatcc
    33181 caatgttcat cttgtgataa atccgcttcg acaaacatta atcgctctgt ggaaatgcca
    33241 ttgtcttgca ttgttttaat tactttatca gctttactta aatcacgtat cgtcgtttgt
    33301 acgacataac cttgttctaa taatcgtgaa ataattcgca tgccaacaaa ccctgtacca
    33361 ccggttacta atactttatt attcatcaca taatctcctc tttgtgtcta ttgattactc
    33421 attatagcag tcattatatt ttgacgtata taaatagact ttagtagcta gtatttaatc
    33481 ttacttatat aaatgaaaat gaatttattc ctttaaaaac tatcattaac cattttcaaa
    33541 tacccaatca tttagaaaca caaacgctat aaatatgatg tctttgctaa ttaactaaat
    33601 tcattttcaa tcgtatgatc aaattacata actacatagt caccatttta aaagcaaata
    33661 cacatattca ttgagggtgt gacattaacg cctaaacaat attaacttaa aatcatctca
    33721 accaatgatt ctaccgtaac attgccaaaa taataaacga tgtctaattg ctttaactgg
    33781 tgcgttaaat cttctcttgt catttttgtt ccttgtaatg cttcttccac atcttttata
    33841 tcaccttgtc caaagaaatc cccataaata cgacaatctg cgatacgatt ttgttcaaca
    33901 gaaattgtta tgtctaccgt acctgatgat aatctttcac ttcgattata ttcatattta
    33961 ggtgacttcc cataattcca atcccaattt ttatactttt tactcgccaa ttcttcaatc
    34021 gcttcccaat ctgcatcaga taattcgtat cgttttgcct ctttaatgtc atcgattccc
    34081 aaaatctgcg tcaccattaa atttttaaat tcttcaattg ttatatcacg atacttttca
    34141 tccagtgctt ctctaagatg accaacacgt gcacgcacag atttaatccc tttcgatgca
    34201 atctttttgc gattaggctt taacacttta tccattgctt cataattaac atcaagtaat
    34261 agcgaataac cgccataaat acgattattc attaatgtca ttgcggcgcc tgacactttt
    34321 ttaccattca atgttaaatc atttctaccg ctttgtacca catctgttgc acctaatgta
    34381 tgcaacgcct ttatagctgg ttgataaaat cgttgaaaat caccataaat tgaagtgtct
    34441 tgttctaaaa tacaacacat attaacggca cctttatcaa catacacagc gccaccacca
    34501 gtatctcggc gaactacttg aatactgtgt ttatctatat aatcttgatt cacttctata
    34561 gcagtatttt gaaaacgtcc aatttccact tttggatcac aataataagg gaataaaata
    34621 tcttcatcta aaaagatatg ctggttaaca taaacttgca tagcgagtgc aatcgcacca
    34681 tcagtaatat attctccatt tctaatcggt tctattaagt acatcttctg tctctccaat
    34741 gttgttattt ttcgttgtgc tgtcgttccg gagtgctact gtaatcaatg ttgagatatc
    34801 ctcagttaaa tgtatggtac gttcttgaat tgaattcgga atgcgatatg cctttttatt
    34861 catcgtcata taaagggcat tttcattttt acgtgtcata cgctgaaaag gatgcttcac
    34921 aaattgtggt gtagtataac caattccaat ttccaaatac aacactttat catcttgatg
    34981 ttgctctaga aaagcattat aacgtcgtag ttgcgcatga aattcagcat cttcaaccat
    35041 gccaacttcc gctttacgtt tattcacttc cattggggca tcacattttg gacatcttgg
    35101 aatcatctcc caaggtataa gcatatcttg ttgcgcaaca accattttac gaattaaatc
    35161 atcattacga tatgtttgag catgacaatg ctgactacac tgttgcagta tatactcccc
    35221 ttgtatatga aatacatgcg tcatatcata ttcagcagca tcgaaagcat tgtctgcatt
    35281 cgtagttatg atatggtaat gtttaccttc cattaaggat tttaaagcga ggtaagactg
    35341 acctacaggt tgatctaaat agtttaatgt aataaaacga ctctcaaacg cccaatactc
    35401 ttgccaactg ccataaggat gtaaactcgc ttgcaacata tcaaagaagc gatatttttc
    35461 aataaaatct gggaaatttt ccgtaaaacg ctctcctaca tatgtaaatc cgtcagatgc
    35521 agacatgcct gcaccaattc caatcactat cgcatctgct tcatcaatcg cagtacgcaa
    35581 tacttcagcc tgctttgtct tttcatccat taaaagagac attgcattcc atttactact
    35641 ctgcatcacg gttcaatgcc tccttataca gttgtaaatc cttatctgta aatacattga
    35701 acacgacttt caatgttgaa tttgtttctt tgagatagct ttctactgtt cgaacagcaa
    35761 tttctgctgc ttcatcttga ggaaaagcaa atacacctgt agatatacag caaaaagcga
    35821 catgatttaa actatgttga tcagccaatt taagacagct aagataacat ttagctaaca
    35881 agtcctgatt catctttgaa acaggcaatc gacgtatttg cggaccaacc gtatgaatta
    35941 tatactttgc tggcaaatta tatccacgtg ttattttggc tttacctaca ccttcattgc
    36001 gcccttgttg tcgaatgatc tctgcacaat caagtcgaac ttgaacaccc gcttttgtat
    36061 gaataatatt atcaatgcag tcatgattag cttgcataca tcctagaaaa cgactatttg
    36121 cagcattaac aatagcatcg atttttaacg tcgtgatatc accttgccaa acaaagatat
    36181 tgtcaccttt tatcggcttc aaatccttca aatccgtaac atgttgatga ttcaaatcac
    36241 ttaacatagc atcttgtaca gctaaatatt catcacttac aggtaatgcc ggtctgacat
    36301 ttgccaagcc tcgatataat tcccacaaat cttcaaatga agatggcatt accaatacgt
    36361 cattgtcatt tctctctcga cgcatatcat tgattaaata ttcaagtctc gctttatttg
    36421 attttaacgt ttccatttaa gcctccggta atgctaggaa tgcttcttta tcgacatcat
    36481 ccaatttgaa caaccaattt tcttctggtt tttcagagtt taaaattgtc ggttcttctt
    36541 ccgcttttgt atttcgctca ataatcgttc ctgacaatgg cgtttgcaca tcaatgaccg
    36601 ttttcgatgc ttcgatactc acaatttcat catccacttt aacttcatct ggacttacga
    36661 attcaacata acctactgtc ccaatatcat cttgcaattc aggtgtcata ctaaacacat
    36721 acaaatctcc tactttttct acccataaat aattggctaa ctttttcatc ttatcttcat
    36781 cctttctctt ctatttaaaa tgctacaaat agatctccgc tagtaatttt aatgttttac
    36841 aacgctgttc aatgcctgga ataagcggtg ctactaacac ctcatcaact tcaaacgtag
    36901 caatgaaatc atctaattgt gctttaacct gttcttgtgt acctgcaatg atgcgtgctt
    36961 gatgtgcttg aatcatctct ttgtctcgat cattaagctt atacttttgc gctgtgtcta
    37021 ccgatggaaa atgttcaaat tctgcaaatt gtaatttacc taataaccaa acatctaagg
    37081 catgttgcaa tgctgctact tccgcttcat tatcagctac aatgacaaat acagatgcca
    37141 tcacctttgc gtccatttta atcgttgatg cttggaaatg ttttttgtaa atatcaatgt
    37201 tatccttcgc tgcatgtatc gcatttatat ctggtagcaa aaatgtgcca acagaaagcc
    37261 ctatacctag ttcggcagct attttggcag atgttgcgct actacttaat aaccacattt
    37321 ctggaaaatg atcaatgtgt ggttggacac ctaacgtatg cgcacttggt ttatccttta
    37381 ttgtaagata atcacgtaat aacgaaatcg attcatcgta actatcatat gtaggattta
    37441 ttccatctaa agcttgcttt accatagtag tacctggatt attaccaata cctaaatcaa
    37501 tacgatttgg atataacgct gccatcattc taaaatgctc agcaatttta taaggtcgat
    37561 agtgcggcag catcacacca ccagagccaa ctcgtatgtg atttgtctgc gccaatgtat
    37621 gcatcatcaa aagttctgga ctactacacg caaacgctgg tacattatga tgttccgtaa
    37681 accaaattcg cttaaagcca agtcgatctg ctaattttgc aagtgtcact gaatcttgca
    37741 atgccttttc tgcatcctta ccttcatcta ttaaggcata gtctaatacg cttaatttaa
    37801 ccaatccgtc atctccaaac ttatcctgtc atgtcaaacc gacataacat tttagcgttc
    37861 ttaataccat tccctcttca tatacccacg tatatgataa cgtttttcaa ttaactttta
    37921 tatcttttcg cctttatttt tctttttcat attcatattt tcagacctac aaatgccatt
    37981 cattagttca attttaaaaa tgttttccat gtaccaattc tattctatca agactgttca
    38041 tcattgatta ttcattcttt ccatggtact ttgaaaacaa tattgaatag aaaaggaatc
    38101 atagtatgta ccgatacaaa ccacttttac aatcaatcca cttacctaat ggcatcaaaa
    38161 tttcgaatcg ctttgtatta tcaccaatga ccgtaaacgc atcaacaaaa gaaggctata
    38221 ttacaaaagc agacttggct tatgctgcac gtcgctctaa ttcagctgga atgcaagtca
    38281 ccggcgctgc ttacattgaa ccttatggtc aattgtttga atatggcttt aatattgatc
    38341 atgatgcttg tatccctgga ttaactaaca tggcatctac gatgaaacaa catggtagcc
    38401 tcgctattat ccagctagcg catgctggtc gtttttcaaa tcaagccatt ttaaattttg
    38461 gaaaggtgta tggaccaagt cctatgactt tgcattcacc gattgaacac gatgttatcg
    38521 ccatgtctca tgagaaaatc aatagcatca ttcagcagta tcgcgatgct acattacgcg
    38581 cgattaaagc aggttttgat ggtgtggaaa tttcaattgc acaacgtttg ctgattcaaa
    38641 catttttctc aactttttcg aataaacgta cagaccatta cggtgccgac tcattaaaaa
    38701 atcgtacaag attatgttta gaagttatgc gtgcggtaca agaagtgatt gataaagaag
    38761 cacctgacaa ttttatttta ggatttcgag caacgccaga agaaaccaga ggcagtgatt
    38821 taggatatac cattgacgag ttcaaccaat taatcgattg ggtcatggat gtttcaaaca
    38881 tccaatattt agcaatcgct agctggggtc gacatattta tcaaaatacg tctcgtacac
    38941 caggtgaaca tttcggtcgg ccagttaacc aaattgtcta tgaacattta gcaggtcgta
    39001 tccctttaat tgcaagtggc gggattaatt ctccagagtc tgctttagat gctttacaac
    39061 atgcagatat ggtcggcatg tcttcgcctt ttgtcacaga acctgacttt gtgcataaac
    39121 ttgctgaaca gcgaccacat gatatcaatc ttgaattttc tatggcagat ttagaagatt
    39181 tagccattcc acatgctgct tttaaagata ttgttaaaat gatggattat ggggaaggtc
    39241 taaagaagca tacccgtgac gctttacgtc aattggaaca gaattatcat gactcatcgt
    39301 cttaagacta tttttcatgg aaatttaaga cagtgttata atgacatcaa tatcaaaaat
    39361 aaggagccta ttaaatcgtg actgaaatta aagataaagt aattactaaa gatgcatttg
    39421 cattacctta cacaattatt aaagcaaaaa atcaaccaac aaaaggcgct attgtctaca
    39481 tccacggtgg tggattaatg ttcggtaaag ctaacgattt atccccacag tatatcgata
    39541 tccttacgga acattacgat ttaattcaac taagttatcg tttattacca gaagtaagtc
    39601 tcgattgcat tattgaggat gtctacgcat cattcgatgc gattcaatca caatattcta
    39661 attgccctat ttttaccttt ggcagatcat caggtgcata tttaagtcta ttaattgcac
    39721 gaaacagaga tattgatggt gttattgatt tttatggtta tagtcgcatc aacactgaac
    39781 cttttaaaac aactaatagt tattacgcaa aaatagctca aagcgtaaac gaaacaatga
    39841 ttgcacaact cacttcacca acaccggttg ttcaagatca aatcgcacaa cgttttttaa
    39901 tctacgttta tgcacgtggc acaggtaaat ggataaatat gattaacatt gctgattata
    39961 ctgattcaaa atataatatt gcaccagatg aacttaaaac acttcccccg gtgttcattg
    40021 cgcattgtaa tggcgattat gatgttcccg ttgaggaaag cgaacatatc atgaatcatg
    40081 ttccacattc aacatttgaa cgcgtgaata aaaacgagca tgattttgat cgtagaccta
    40141 atgacgaagc aatcactatt tatcgtaaac ttgttgattt cttaaacgct ataacaatga
    40201 tgtaatatat cattatttta aaaaactcaa tttatcatca tgataaacag ttattcgatc
    40261 aaccatatta ttcatacaat ttattaagct cataaacaat acgcctgtcc ctttagtttt
    40321 cgctaatgag acaggcgctt tatttcactt tactattttg aaattcaaca ctgtgatgct
    40381 acttatatct gctgtgccat gttagtagca cgatttacat agcatattaa attcagaaga
    40441 tgaatttaac ttgctttcaa ttgtgttcct ttactttcag ttgcttcaac acgcaacaag
    40501 tcatttataa tacctgtata gaagttggca agttctgcac ctttacgctt gaaatctagg
    40561 aaatccacac caataaaatc gacatgatcc catccttgta tgattggttt aacttgccag
    40621 atacctctgc gtgtggcagg ttcatcattc gtaacattaa caaatggttg attggacgga
    40681 tgtaatgacg aaattactgg tacgacacca tcatttttac gccattcttc tcttgcatca
    40741 tgaccaataa ttctactcgt tgtatccatt aagaaaaatg tacctaaatc aggattttca
    40801 taacctaatg gaccagtatg agatgataca cctgtataag tcgtatacgt aatattagga
    40861 ttcatacttg tcatgttgtt caattttgca gagccatcta acgttaaatc ataggcagca
    40921 ttgtcgtctg atgtccaaat tttgctttta ctaacgcgtt ttatatagtc aatgtaactc
    40981 tcatttggta attgtttaaa gccccattgc gttaatccta aatcgatatt cgaatacttg
    41041 ttacccataa atcgatttaa agcgaacatg atttttctaa cagcttctgt atttccaaac
    41101 ttatcagctg cttgtgaacc attatgtggt gttgctaatg ttgtgattga tgcaaccata
    41161 ttgttatgac caccagtgaa taatggtgat atttctccac catgcgcttt atgataggca
    41221 atttcttctt tgttaccatt tcttaaaaac tcttccatta aacgaattgt ttgaccaccc
    41281 atactatgcc ctacaagatg taccttttta ccaggttccc aattaggcat gattccttta
    41341 taagtcttac catagcgctc atgtccgtat ttagctgcat gtgctgcgcc ataatctacg
    41401 cgaccacctt taatgtaata ataaagttct acagcgcgat catagttact accaaatgca
    41461 cttacacttg cttgatgtac attatagcct tgctttctca attcttcgat aactttaaat
    41521 ttatttccac cccaataatt tggatataaa gcaggtgcat tatcgcctac taatcctaaa
    41581 aatccatgta caaaaacaac tggatatttg ttaagtggtt gtacttgatt cgctttaagt
    41641 ggtcgaacct ttttagcatc tgctgctgca tttgtagtcg aacgtgtttt aggcgcttga
    41701 agcgcatctt tgattagttg tttattatca gtatttttat ctgtcggttt ctctgggaaa
    41761 ttttgaccat cgatatgttt acttaaatcg ccaacttgtg gtttctgttc actttggcga
    41821 acagtatcat cttgctttgc atctgtgatt tgatgcgcgg gttgatgtgt atcttgttga
    41881 tttggatgtt tgtccgtggt tgcatcttgt gcttttgttg atttaggtgc agttttatca
    41941 ttagacgggg gtgtagttga agttggtgct gctttatcat gattgggtat taccttttgt
    42001 gccggaatgg atggttgtga tttatcaaca tcattcgatt gtgacgttgt cgattgtgat
    42061 ccattacgtt cagcatgatg ggtattttga acctgatcat tttgacgttc agttttgtta
    42121 gtcgcattgt tttggtgtga ctgtttagaa ccttgtcgtt cagaatcttg ctttacttga
    42181 ttatcatttt gcgttcggat tgttttacta ttttgtgttg attgatgctt cgatgattga
    42241 agcgtgtctt taccttctgt cactgttcca cttttaccat tctctttatg catatcgtct
    42301 aattgctttc ctgactgcat ttgatgtgac gttatcgcat tcccttgttc tcccggttga
    42361 tttagtgttt ctttttgtgc cgctgcatta gttggtgttt tttccgaggc ttgtgcttca
    42421 tgtgatgaca caacaaacat tgtagccgct aacactgaca ccacgcctat tgaatacttt
    42481 ctaatactat actttctttc ttcttgtcct cttaacatat aatcacctca aaagtatttt
    42541 tttgaccccc tcttgagaat gaaaaacaac tgcaccttca atataacatg acatatatta
    42601 ttttttg
//
LOCUS       DAHHYJ010000007        42005 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000007 DAHHYJ010000000
VERSION     DAHHYJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 42005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42005
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.007"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..536)
                     /locus_tag="NBK22_000295"
     CDS             complement(<1..536)
                     /locus_tag="NBK22_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2413512.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVN"
     gene            640..1527
                     /gene="pdxS"
                     /locus_tag="NBK22_000296"
     CDS             640..1527
                     /gene="pdxS"
                     /locus_tag="NBK22_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCD2413513.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            1531..2091
                     /gene="pdxT"
                     /locus_tag="NBK22_000297"
     CDS             1531..2091
                     /gene="pdxT"
                     /locus_tag="NBK22_000297"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCD2413514.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVDILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(2299..3513)
                     /locus_tag="NBK22_000298"
     CDS             complement(2299..3513)
                     /locus_tag="NBK22_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD2413515.1"
                     /translation="MHILIGIIGIIFFLTLAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            3671..4132
                     /locus_tag="NBK22_000299"
     CDS             3671..4132
                     /locus_tag="NBK22_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCD2413516.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            4151..4717
                     /locus_tag="NBK22_000300"
     CDS             4151..4717
                     /locus_tag="NBK22_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCD2413517.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            4707..5714
                     /locus_tag="NBK22_000301"
     CDS             4707..5714
                     /locus_tag="NBK22_000301"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCD2413518.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            5728..8184
                     /locus_tag="NBK22_000302"
     CDS             5728..8184
                     /locus_tag="NBK22_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCD2413519.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            8669..10033
                     /gene="radA"
                     /locus_tag="NBK22_000303"
     CDS             8669..10033
                     /gene="radA"
                     /locus_tag="NBK22_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCD2413520.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            10058..11131
                     /locus_tag="NBK22_000304"
     CDS             10058..11131
                     /locus_tag="NBK22_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCD2413521.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
     gene            11688..13142
                     /locus_tag="NBK22_000305"
     CDS             11688..13142
                     /locus_tag="NBK22_000305"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCD2413522.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHSHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEALEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            13565..14212
                     /gene="cysE"
                     /locus_tag="NBK22_000306"
     CDS             13565..14212
                     /gene="cysE"
                     /locus_tag="NBK22_000306"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCD2413523.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            14196..15596
                     /locus_tag="NBK22_000307"
     CDS             14196..15596
                     /locus_tag="NBK22_000307"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCD2413524.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            15589..15993
                     /locus_tag="NBK22_000308"
     CDS             15589..15993
                     /locus_tag="NBK22_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCD2413525.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            16001..16747
                     /gene="rlmB"
                     /locus_tag="NBK22_000309"
     CDS             16001..16747
                     /gene="rlmB"
                     /locus_tag="NBK22_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCD2413526.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            16747..17271
                     /locus_tag="NBK22_000310"
     CDS             16747..17271
                     /locus_tag="NBK22_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCD2413527.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARIQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            17352..17921
                     /locus_tag="NBK22_000311"
     CDS             17352..17921
                     /locus_tag="NBK22_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCD2413528.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            18036..18179
                     /gene="rpmG"
                     /locus_tag="NBK22_000312"
     CDS             18036..18179
                     /gene="rpmG"
                     /locus_tag="NBK22_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD2413529.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            18235..18417
                     /gene="secE"
                     /locus_tag="NBK22_000313"
     CDS             18235..18417
                     /gene="secE"
                     /locus_tag="NBK22_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCD2413530.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            18430..18978
                     /gene="nusG"
                     /locus_tag="NBK22_000314"
     CDS             18430..18978
                     /gene="nusG"
                     /locus_tag="NBK22_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCD2413531.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEETQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            19159..19581
                     /gene="rplK"
                     /locus_tag="NBK22_000315"
     CDS             19159..19581
                     /gene="rplK"
                     /locus_tag="NBK22_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCD2413532.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVATVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            19789..20481
                     /gene="rplA"
                     /locus_tag="NBK22_000316"
     CDS             19789..20481
                     /gene="rplA"
                     /locus_tag="NBK22_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCD2413533.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    20569..20715
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            20753..21253
                     /locus_tag="NBK22_000317"
     CDS             20753..21253
                     /locus_tag="NBK22_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCD2413534.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEENAE"
     gene            21296..21664
                     /gene="rplL"
                     /locus_tag="NBK22_000318"
     CDS             21296..21664
                     /gene="rplL"
                     /locus_tag="NBK22_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCD2413535.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            21839..22447
                     /locus_tag="NBK22_000319"
     CDS             21839..22447
                     /locus_tag="NBK22_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2413536.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGSSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKLNGIDNVIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            22662..26213
                     /gene="rpoB"
                     /locus_tag="NBK22_000320"
     CDS             22662..26213
                     /gene="rpoB"
                     /locus_tag="NBK22_000320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCD2413537.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            26377..29973
                     /gene="rpoC"
                     /locus_tag="NBK22_000321"
     CDS             26377..29973
                     /gene="rpoC"
                     /locus_tag="NBK22_000321"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCD2413538.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            30110..30364
                     /locus_tag="NBK22_000322"
     CDS             30110..30364
                     /locus_tag="NBK22_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCD2413539.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            30462..30875
                     /gene="rpsL"
                     /locus_tag="NBK22_000323"
     CDS             30462..30875
                     /gene="rpsL"
                     /locus_tag="NBK22_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCD2413540.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            30941..31411
                     /gene="rpsG"
                     /locus_tag="NBK22_000324"
     CDS             30941..31411
                     /gene="rpsG"
                     /locus_tag="NBK22_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCD2413541.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            31534..33615
                     /gene="fusA"
                     /locus_tag="NBK22_000325"
     CDS             31534..33615
                     /gene="fusA"
                     /locus_tag="NBK22_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCD2413542.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            33832..35016
                     /gene="tuf"
                     /locus_tag="NBK22_000326"
     CDS             33832..35016
                     /gene="tuf"
                     /locus_tag="NBK22_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD2413543.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(35298..36473)
                     /locus_tag="NBK22_000327"
     CDS             complement(35298..36473)
                     /locus_tag="NBK22_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD2413544.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFFKIIELEQVFKTN"
     gene            36644..37831
                     /locus_tag="NBK22_000328"
     CDS             36644..37831
                     /locus_tag="NBK22_000328"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCD2413545.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            38099..38977
                     /gene="hchA"
                     /locus_tag="NBK22_000329"
     CDS             38099..38977
                     /gene="hchA"
                     /locus_tag="NBK22_000329"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCD2413546.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNEDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            39136..40773
                     /locus_tag="NBK22_000330"
     CDS             39136..40773
                     /locus_tag="NBK22_000330"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCD2413547.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPHTYALQNSNDYLEIMEEGISYIVRESKIDPDNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNIRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEAVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            40987..41952
                     /locus_tag="NBK22_000331"
     CDS             40987..41952
                     /locus_tag="NBK22_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD2413548.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
BASE COUNT        15093 a         6157 c         8202 g        12553 t
ORIGIN      
        1 tgatttacta attgttcggt agatgagcca attataattt gattaggatc acaaattacg
       61 ccacgattag taaataaata aaatgccagt tgaaaccgca aatgtaattc tccttgaaaa
      121 tgtcctctac gtaattgatt taaatgattt gtatcataaa gatctttgga atactttctg
      181 aaaagttcta tagggaaatg tttcgtatct atttcatcca aattaaaagc ataatcataa
      241 gcttcatcac tcgcttttgg tttatatgaa tcatcatcaa aaagagaggg gataggttga
      301 ttgtttaaaa ttgttaaaga ttcaatttcg gacacaaaat atccagagcg aggtcttgaa
      361 taaatgtaac cttcgtctaa tagaagttga tatgcatgct ctacggttgt ttggctaata
      421 gataaatgtt tgcttaattg tcttttagaa taaaatttat caccttcttt aaattgacct
      481 tcaattattt gtttttttaa tttttcataa agttgatggt ataaagtgtt tttcaatttt
      541 ataactgacc tcctaaattt atcttatttt gtaccttttt aaatatcagt ttatacatta
      601 caatgtattt aatcaacttg aaaaggggtt ttatgtataa tgagtaaaat tattggatca
      661 gacagagtca aaagaggtat ggctgaaatg caaaaaggcg gcgttattat ggatgtcgtt
      721 aatgctgagc aagcaagaat tgcagaagaa gctggcgcgg tagcagttat ggcattagaa
      781 cgagtacctt ctgatattag agctgctggt ggcgttgcac gtatggcaaa ccctaaaatt
      841 gtagaagaag taatgaatgc tgtttctatt ccagtcatgg ctaaagcacg tattggtcat
      901 atcactgaag caagagtatt agaggcgatg ggtgttgact atattgatga atcagaagtg
      961 ttaacaccag cagatgagga atatcactta agaaaagatc aatttacagt accatttgta
     1021 tgtggatgtc gtaatttagg tgaagctgcg cgtagaattg gtgaaggtgc tgctatgtta
     1081 cgtactaaag gtgaaccagg tacaggtaat attgttgaag ctgtaagaca tatgagacaa
     1141 gttaattcag aagttagtcg attgactgta atgaatgatg atgagattat gacttttgcg
     1201 aaagatatcg gtgcgcctta tgaaatttta aaacaaatta aagacaatgg tcgtttaccg
     1261 gtagttaact ttgcagctgg tggcgttgcg actcctcaag atgctgcttt aatgatggaa
     1321 ttaggtgccg acggtgtgtt cgttggatca ggtattttta aatcagaaga tccagaaaaa
     1381 tttgctaaag caattgttca agcaacaaca cattaccaag actatgaact aattggaaga
     1441 ttagcaagtg aacttggcac tgctatgaaa ggtttagata tcaatcaatt atcattagaa
     1501 gaacgtatgc aagagcgtgg ttggtaagat atgaaaatag gtgtattagc attacaaggt
     1561 gcagtacgtg aacatattag acatattgaa ttaagtggtc atgaaggtat tgcagttaaa
     1621 aaagttgaac aattagaaga aatcgagggc ttaatattac ctggtggcga gtctacaacg
     1681 ttacgtcgat taatgaattt atatggattt aaagaggctt tacaaaattc aactttacct
     1741 atgtttggta catgcgcagg attaatagtt ctagcgcaag atatagttgg tgaagaagga
     1801 taccttaaca agttgaatat tactgtacaa cgaaactcat tcggtagaca agttgacagc
     1861 tttgaaacag aattagatat taaaggtatc gctacagata ttgaaggtgt ctttataaga
     1921 gcgccacata ttgaaaaagt aggccaaggc gtagatatcc tatgtaaggt taatgagaaa
     1981 attgtagccg tccagcaagg taaatattta ggcgtatcat tccatcctga attaacagat
     2041 gactatagag taactgatta ctttattaat catattgtaa aaaaagcata gcttaatgta
     2101 tgctaaatca acgaattatt gatatttata gatttgttga gaagacaata tctccttcaa
     2161 acttagcttt ggaggagttg ttttttatgt caaaattaaa aatgataaaa aataaagata
     2221 tacataagaa aaaacccctt caaagacact gagaatagtc aaaattttga aggggttaat
     2281 tcgatgttga tgtatttgtt aaataaagaa tccagcgatt gcagctgaaa tgaaagatac
     2341 tagtgttgca ccgaataata gtttcaaacc aaagcgggca actgtatctc cttttttgtc
     2401 attaagagat ttaatcgcac ctgaaataat accaatagag ctaaagttag caaatgatac
     2461 taagaataca gatgtaacac cttttgcgtg ttcagataaa tcactaagtt taccaagtgc
     2521 ttgcattgct acaaattcgt tagataatag ttttgtcgcc ataactgaac cggcttgaac
     2581 tgcatcttgc catggcacac cgactaagaa tgcaaatggt gcaaagacaa aaccaattaa
     2641 tgtttggaaa tcccaagaaa tagcgccacc tgaaactgta ctaaagatat tgcttacaat
     2701 tccatttaat agagcgataa tagcaatgta tccgattaac attgcgccta caatgacagc
     2761 tactttaaat ccatctaaaa tatattctcc tagcatttcg aagaatgatt gttgtctttc
     2821 ttcagtttct tcaactaata atttgtcatc ttcttcatta actttataag ggttaataat
     2881 tgaagcgatg atgaaaccac caaataagtt taagacaaca gccgttacaa catatttagg
     2941 ttcaattaag gtaaagtatg caccgataat tgaagcagaa acagtcgaca ttgctgaagc
     3001 tgttaatgtg tataaacgtt gcttaggtat gtatggtaat tgttttttaa ttgaaataaa
     3061 tacttcagat tgtcctaaaa ttgctgcagc aactgcattg tatgattcta aacgtcccat
     3121 accattaatt ttagaaatta agaatcctaa aacattaatg attaaaggta aaatctttgt
     3181 gtattgaagg ataccgataa tcgctgaaat aaatacgata ggtaataata cactgaagaa
     3241 gaatggtggt tgcttaggat cgatatattg aataccaccg aatacaaagt taacaccatc
     3301 tgctgctttt aataataagt agttaaaacc gtttgaaata ccaccaataa ccttgattcc
     3361 cattgtagtt ttaagcaaga taaatgcaaa gataagctga attgcaagta aaattcctac
     3421 atatttccag cgaatatttt tcctgtctga gctaaataga aacgcaagtg ttaaaaagaa
     3481 gataattccg ataatcccaa ttagaatatg catatatttc tcattccttt agttttttct
     3541 acaatctatc atacaataaa atggaagggc taacatcata aatttttgaa aatataaaaa
     3601 caaattaatt gaaaaaggtc aaaataggtc atataatata gtcaaagaag gtcaaaaagg
     3661 ggtgatatac atgcacaata tgtctgacat catagaacaa tacatcaaac gtttatttga
     3721 agagtcgaat gaagatgtcg ttgaaattca gagagcgaat atcgcacagc gttttgattg
     3781 tgtaccatca caattaaatt atgtaatcaa aacacgattc actaatgaac atggttatga
     3841 aatcgaaagt aaacgtggtg gtggtggtta catccgaatc actaaaattg aaaataaaga
     3901 tgcaacaggt tatattaatc atttgcttca gctgattgga ccttctattt ctcaacaaca
     3961 agcttattat attattgatg ggcttttaga taaaatgtta ataaatgaac gtgaagctaa
     4021 aatgattcaa gcagttattg atagagaaac gctatcaatg gatatggttt ctagagatat
     4081 tattagagca aatattttaa aacgtttgtt accagttata aattattact aaatgaaatg
     4141 aggtgttgaa gtgctttgtg aaaattgtca acttaatgaa gcggaattaa aagttaaagt
     4201 tacaagtaaa aataaaacag aagaaaaaat ggtgtgtcaa acttgtgctg aggggcacca
     4261 tccgtggaat caagctaatg aacagcctga atatcaagaa catcaagata atttcgaaga
     4321 agcatttgtt gttaagcaaa tcttacaaca tttagctacg aaacatggca ttaattttca
     4381 agaagtagcg tttaaagaag aaaaacgttg cccatcatgt catatgactt tgaaagatat
     4441 tgcacatgtt ggtaaatttg ggtgtgctaa ttgttatgca acatttaaag atgacatcat
     4501 tgatatcgtc cgcagagttc aaggtggaca atttgagcac gttggaaaga caccacattc
     4561 ttcacataaa aagatagctt taaagcgaaa aatcgaagaa aagaatgaat atttgaaaaa
     4621 acttattgaa atccaagatt ttgaggaagc agccattgtt agagatgaaa ttaaagcact
     4681 aaaagctgag agtgaggtgc aacatgatga cgcataatat tcatgataat atcagccaat
     4741 ggatgaaaag taatgaagaa acaccaattg ttatgtcttc tagaattcgg ttagcgcgta
     4801 atttagaaaa tcatgtgcat ccactaatgt atgctactga aaatgatgga tttagagtta
     4861 taaatgaggt acaagatgcc ttgccaaatt ttgaattaat gcgtcttgat caaatggatc
     4921 aacaaagtaa aatgaaaatg gttgcaaagc atttgattag tcctgaacta ataaaacaac
     4981 cagcagccgc agtattagtg aatgatgatg aatctttaag tgtcatgata aatgaagagg
     5041 accatattcg tattcaagct atgggaactg acacgacatt acaggcttta tataatcaag
     5101 cttcatcaat tgatgatgaa ttagatcgaa gccttgatat aagttatgat gaacaacttg
     5161 gttatttaac tacatgtcct accaatatag gtactggtat gagagcaagc gtgatgctac
     5221 atttgccagg tctatctatt atgaaaagaa tgacacggat tgctcaaacc attaatcgtt
     5281 ttggatatac aatcagaggt atttacggtg aaggttcgca agtttatgga catacttatc
     5341 aagtatccaa ccaacttaca cttggtaaat ctgagttaga aatcatagaa acattaacag
     5401 aagttgttaa tcaaatcatt catgaagaaa aacaaatacg acaaaagtta gacacttata
     5461 atcaattaga aacacaagac cgtgtttttc gctcgctagg tattttacaa aactgtagaa
     5521 tgataactat ggaagaggct tcttatagat taagcgaagt taaacttggt atagatttaa
     5581 attacattga attacaaaac tttaaattta atgaattgat ggtagctata cagtcaccat
     5641 ttttattaga tgaagaagat gacaaatctg taaaagaaaa acgagcagat atactaagag
     5701 aacatataaa gtaggaggtc attatttatg ttatttggta gattaactga gcgtgcacag
     5761 cgcgtactag cacatgcaca agaagaagca attcgtttaa atcattcaaa tataggaaca
     5821 gaacacctat tattgggatt aatgaaagag cctgaaggaa ttgctgcaaa agtattagaa
     5881 agttttaata tcactgaaga taaagtaatt gaagaagttg aaaaattaat tggacatggt
     5941 caagatcatg ttggtacatt gcattataca cctagagcta aaaaagtcat tgaattatcg
     6001 atggatgaag ctagaaaatt acatcacaat tttgttggaa cggaacatat tttattaggc
     6061 ttgattcgtg aaaatgaagg tgttgcagca agagtttttg caaatctaga tttaaatatt
     6121 actaaagcac gtgcacaagt tgtgaaagct ttaggaaacc ctgaaatgag taataaaaat
     6181 gcacaagcta gtaagtcaaa taatactcca actttagata gtttagctcg tgacttaaca
     6241 gtcattgcca aagacggtac attagatcct gttataggac gtgataaaga aattacacgt
     6301 gtaattgaag tattaagtag acgtacgaaa aacaatcctg tgcttattgg agagccaggt
     6361 gttggtaaaa ctgctattgc tgaaggttta gcgcaagcca tagtgaataa tgaggtacca
     6421 gagacattaa aagataagcg tgttatgtct ttagatatgg gaacagtagt tgcaggtact
     6481 aaatatcgtg gtgaatttga agagcgtctg aaaaaggtta tggaagaaat ccaacaagca
     6541 ggtaatgtca tcctatttat tgatgagttg catactttag ttggtgctgg tggtgctgaa
     6601 ggtgctatcg atgcttcgaa tattttgaag ccggcattag cacgtggtga attacaatgt
     6661 attggtgcta ctacattaga tgaatatcgc aaaaatattg aaaaagacgc ggctttagaa
     6721 cgtcgtttcc aacctgtaca agttgatgaa ccttcagtag tagatacagt tgctatttta
     6781 aaaggattaa gagatcgtta cgaagcacac catcgtatta atatttcaga cgaagctatt
     6841 gaagcagctg ttaaattaag taacagatac gtttcagatc gtttcttacc agataaagca
     6901 attgatttaa ttgatgaagc aagttctaaa gtaagactta agagtcatac gacacctaat
     6961 aatttaaaag aaattgaaca agaaattgaa aaagttaaaa atgaaaaaga tgccgcagta
     7021 catgctcaag agtttgaaaa tgctgctaac ctgcgtgata aacaaacaaa acttgaaaag
     7081 caatatgaag aagctaaaaa tgaatggaag aatgcacaaa atggcatgtc aacttcattg
     7141 tcagaagaag atattgctga agttattgca ggatggacag gtatcccatt aactaaaatc
     7201 aatgaaacag aatctgaaaa acttcttagt ctagaagata cattacatga gagagttatt
     7261 gggcaaaaag atgctgttaa ttcaatcagt aaagcggtta gacgtgcccg tgcagggtta
     7321 aaagatccta aacgaccaat tggtagcttt atcttccttg gaccaactgg tgttggtaaa
     7381 actgaattag ctagagcttt agctgaatca atgtttggcg atgatgatgc gatgatccgt
     7441 gtagacatga gtgaatttat ggaaaaacac gcagtgagcc gattagttgg tgctcctcca
     7501 ggatatgttg gtcatgatga tggtggacaa ttaactgaaa aagttagacg taaaccatat
     7561 tctgtaattt tatttgatga aattgaaaaa gctcatccag atgtatttaa tattctatta
     7621 caagttttag atgatggaca tttgacagat acaaaaggac gtacagttga tttcagaaat
     7681 acaattatca taatgacatc aaacgttggg gcacaagaat tacaagatca acgatttgct
     7741 ggattcggtg gttcaagtga tggacaagat tatgaaacaa ttcgaaaaac gatgttaaaa
     7801 gaattaaaaa attcattccg tccagaattt ttaaaccgtg tagatgatat cattgtattc
     7861 cataaactaa caaaagaaga attaaaagaa attgtaacaa tgatggttaa taaattaaca
     7921 aatcgattat ctgaacaaaa cataaatatt attgttactg ataaagcgaa agacaaaatc
     7981 gcagaagaag gatatgatcc agaatatggt gcaagaccat taattagagc gatacaaaaa
     8041 actatcgaag ataatttaag tgaattaata ttagatggta atcaaattga aggtaagaaa
     8101 gttacagtag atcatgatgg taaagagttt aaatatgaca ttgctgaaca aacttcagaa
     8161 actaaaacac catcgcaagc ataattataa aacagtccaa aacaaattaa agttttgggc
     8221 tgttttttta gtagcattga actatagaaa ttcgtgaaag tatccatcaa cgaaacaatc
     8281 taataaaaca atcatcaaag gatagttaag aattatatgt aacaagttaa tgagcctaca
     8341 gcgatagcat aaaggtatga atttttataa aggttttttg tttgaagata ctagcaactt
     8401 acgtcaaaat aaaataggtc tatataatat tgtgataaat ctagagaaaa gagtttacta
     8461 agaaattatt aagattttat ctttgaaaga taccgactat caacatgatg taagtttatt
     8521 ttataatatt cataaaaaat aaatctggta aaacagtttg cgttagtagt catgttaaaa
     8581 tacaaacaaa tgtaactcac atttaatttg tcataatggg aatgtgcgtt taaaatagat
     8641 ttgttcaaag gaaagtggag gtgcaatttt ggccaagaaa aaagtgattt ttgaatgtat
     8701 ggcttgtggt tatcaatctc ctaaatggat ggggaaatgt cctaattgtg gcgcttggaa
     8761 tcaaatggag gaaattgttg aaaaagcagc caatcctaaa catggagtta aaaccaagga
     8821 attagcaggt aaagtacaaa aattaaatag tattaaacat gaaacaacgc cgagagtgtt
     8881 aacagattca gcagaattca accgtgtatt aggtggaggt attgtgagcg gatcgttagt
     8941 acttattggt ggggatccag gtattggtaa gtcaacgtta cttttacaaa tttgtgcatc
     9001 gttatctcaa aagaaaaaag tactatatat tactggagaa gaatcgctta gtcagactaa
     9061 attacgtgca gagcgattag atgaagattc aagtgaattg caagtattag ctgaaacaga
     9121 tcttgaagtt atttatcaaa cagtaaaaga agaacaacct gatttattag tagtggattc
     9181 gattcaaaca atatatcatc ctgaaatcag ctctgcgcca ggttctgttt cacaagttcg
     9241 tgaaagtaca caaagtttaa tgaatattgc taaacaaatg aacattgcaa cttttatagt
     9301 gggtcatgta acgaaagaag gtcaaattgc tggcccaaga ttgctagaac acatggttga
     9361 tactgtgctt tattttgaag gcgatgaaca ccacgcatat cgaattttgc gagctgttaa
     9421 aaaccgtttt ggttcaacga atgaaatggg aatcttcgaa atgaagcaaa gtggattaaa
     9481 aggtgtaaat aatccatctg aaatgttttt agaagaacgt tcaacaaatg ttccaggttc
     9541 aacaattgtt gcaaccatgg agggaaccag accactttta atagaagttc aagcgctggt
     9601 aactccaacg acttttaaca atccgagacg aatggcaaca gggattgatc ataatcgatt
     9661 aagtttgttg atggctgttt tggaaaagaa agaaaattat ctattacaac aacaagatgc
     9721 ttatatcaaa gtagctggcg gtgtaaagtt aacggagcca gcagttgatt taagtgtaat
     9781 tgtagcaact gcatctagct ttaaagataa agctgtcgac ggattagatt gctatattgg
     9841 agaagttggt ttaacgggtg aggtacgtcg tgtatctcgg atagaacaac gcgtgcaaga
     9901 ggctgcaaaa ctaggtttca aacgtgtaat tattcctaaa aataatatag gcggatggac
     9961 atatcctgaa ggtatacaag taataggtgt aactactgta catgaagcat tgtcatttgc
    10021 tcttcattca taaaacatca agaaaggagg acattgtgtg aatatcgtta aactaatggt
    10081 tattattatt tacttaatta ttgggagcgc attaggaata attattattc ctgaaattgc
    10141 aaatgatctt ggattacaaa actccagctt tttaaaaaat cactatgtag atggcattat
    10201 cggtagtatt tttatgttct taatttttgg tgtatttatt agacgagtta ctaacgctat
    10261 aaaaggttta gaacatttta ttatgcgtag aagtgctgtt gaaatactat tcgcaacaat
    10321 aggtttaata atcggattac ttatttctgt tatggtgtcg tttatattag aatcaattgg
    10381 taactctatt tttaatcatt tcattcctgt cataattacg atattactat gttatttcgg
    10441 tttccaattt ggccttaaaa aacgagatga aatgttaatg tttttacctg agaatatagc
    10501 gcgttccatg tcacaacata ctaaaagtgc tacgccaaaa attatcgaca caagcgcaat
    10561 tattgatggt cgtattttag aagtcattcg ttgcggtttt atcgatggca atattttaat
    10621 tccgcaaggt gttattaatg aattacaaat tgttgcagat tcaaatgaca gtgttaaacg
    10681 tgaaaagggt aaaagaggct tagatatttt aaatgaattg tatgatttag attatcctac
    10741 aaaggttata catccaacta aaacacatag tgatattgat acaatgctat taaagctagc
    10801 taaacaatat catgcaagta ttattacgac agatttcaac ctaaataaag tttgtcatgt
    10861 acatggtatc aaagcattaa atgttaatga tttatcagaa gcaatcaaac ctaatgtaca
    10921 tcaaggtgat caactgcata ttttactgac aaaaatgggt aaagagcctg gtcaggcagt
    10981 aggatatcta gatgatggta cgatggtagt tgttgataat gctaaaaatc ttattggcag
    11041 tcatgtcaat ttagaagtag tcagcttatt gcaaacatct tcaggaagaa ttgtttttgc
    11101 taaaaaaatc gaagatacag tatcattata aaaactgtag ttgacgaaaa caaaatagat
    11161 attaaaaggt atatgaaatg gcgaagtttg taatttgaag tataactata aataaatgca
    11221 aaacatatta gttatcaaaa aatacaaata gtgctaatat atcattgaaa caagttcatt
    11281 aagtgttaaa ttttagatat taataaagtt attcttatag caatcaagac atacaaaatt
    11341 agaatcaatt ttaacgtatt tgaatagtta atttgaagtt taatttcaca gtacataata
    11401 ttcattttta agtcgttgaa atgtttatgt agtgcagtct tgattcttag ggatagcttt
    11461 tttaaagtgt tgaaaaaaca actgctttct atttaaaatt taagcatatt acccagggaa
    11521 ttatgttatg attaaacaat gaaattattt taaggtcatg ttaaaaagca ttgtttcgtt
    11581 ttaacaataa gacgagttag acatttgtta tgacggtcga agatagcaaa tgaacaattt
    11641 atatgaacta acttgaaaat ctgatattta atttaggagt gaaaaaaatg agcgatcgta
    11701 taagagtaag atatgcacca agtccaactg gttatcttca tattggtaat gcaagaacag
    11761 cattattcaa ttacttgtat gctaaacatt acaacggaga ttttgtgatt cgaattgaag
    11821 atactgataa aaaacgtaat ttagaagatg gagaaacatc acaatttgat aatcttaaat
    11881 ggttaggatt agattgggat gagtctgtag ataaagacaa tggctacgga ccatatcgtc
    11941 aatctgaacg tcaacatatc taccaaccat taatagatca gttactagca gaagataaag
    12001 catataaatg ctatatgaca gaagaagaat tagaagctga acgtgaagcg caaatcgctc
    12061 gtggtgaaat gcctcgctat ggtggtcaac attcgcattt gactgaagaa caacgtcaac
    12121 aatttgaagc agaaggacgc caaccatcaa ttcgtttccg agtacctcaa aaccaaacgt
    12181 attcatttga tgatatggta aaaggaaata tttcatttga ttcaaatggt attggtgact
    12241 gggttatcgt aaaaaaagat ggcattccaa cgtacaattt tgcagtagct atagatgatc
    12301 attacatgca aatttcagat gtaattcgtg gtgatgatca tatttcaaac acgcctaaac
    12361 aaattatgat ttatgaagca tttggctggg agccacctcg ttttggtcat atgtcattaa
    12421 ttgttaatga agaacgtaaa aagttaagta aacgtgatgg gcaaatttta caatttattg
    12481 agcaatatcg tgacttaggt tatttacctg aagcgttatt taattttatt gcgttattag
    12541 gttggtctcc tgaaggtgaa gaagaaatct tttctaaaga agaatttatc aaaatctttg
    12601 atgaaaagcg tttgtcaaaa tcaccagcat ttttcgataa gcaaaaatta gcatgggtta
    12661 ataaccaata tatgaaacaa aaagatactg aaacagtatt ccaattagca ttacctcatt
    12721 taattaaagc aaatttgatt cctgaggtgc cgtcagaaga ggatttatct tggggacgca
    12781 aattaattgc gctttatcaa aaagaaatga gttatgccgg tgaaattgta cctttatcag
    12841 aaatgttctt taaagaaatg ccagctcttg gtgaagaaga acaacaagtg attaatggag
    12901 agcaagtacc agagttaatg acgcacttat tcagtaaatt agaagcactt gaaccatttg
    12961 aagcggctga aattaaaaag acaattaaag aagttcaaaa agaaacagga ataaaaggca
    13021 agcaattatt tatgcctatt cgtgttgctg taacaggcca aatgcatggt cctgaattac
    13081 caaatacaat tgaagtactt ggtaaagaaa aagtgctaaa ccgtttaaaa caatataagt
    13141 aatgaaatag acaagatcaa ggtagtatat acttgaattt tataacgtaa ccacatatga
    13201 taaagttgta ttaacaaatt acaacaatat gaataaagta ttttatataa aacgaaggat
    13261 tagtaattaa atttatacga tgcagagagt gtacggttgc tgtgagtaca acgtagaaat
    13321 taatgaatgc accttcgtaa aatgaattaa atatataatg agagtgatga gcattaagtt
    13381 gacttagttt ccttgataat ttggaagcgc ccgcaatatt attaatgtta ttcgctaaat
    13441 tcagagtgga accgtgcgga agcgcctcta acaatacaat ttgtatgtta gtggtgcttt
    13501 tttgatattt aatttcgcag gtacttcaat tatgtattta cgtagttcaa tcattgagga
    13561 ggaaatgatc ttgttaaaaa gaatgagaga cgatataaaa atggtatttg agcaggatcc
    13621 agcggcacgt tcaacattag aagtcattac aacgtatgca ggtttacatg cagtttggag
    13681 tcatttgatt gcacataagt tatacaacca aaaaaaatat gttgcagcac gcgcgatatc
    13741 tcaaatttca agatttttca caggtataga aatccatcca ggtgctaaaa ttggaaagcg
    13801 tctatttata gatcatggta tgggcgttgt aataggagaa acatgtacaa ttggtgataa
    13861 tgtgacaatc tatcaaggcg tgacacttgg tgggacaggg aaagaaagag ggaaaagaca
    13921 cccagatata ggagacaatg ttttaatagc agcgggtgcg aaagttttag gaaatattaa
    13981 aataaattca aatgtaaata ttggtgcaaa ttcagttgtt ttacaatcag ttccaagcta
    14041 ttcaacggtt gttggtatac caggacatat tgttaagcaa gatggtgttc gagttggaaa
    14101 aacatttgat catcgccatc tacctgatcc aatttatgaa caaattaagc atttagaacg
    14161 acaacttgaa aagactagga atggagagat tcaagatgat tacattatat aatacgctta
    14221 cacgtcaaaa agaagtgttc aagcctatag aaccaggaaa agtaaaaatg tatgtatgtg
    14281 gtcctactgt atataactac attcatattg gtaacgcaag accagcaatt aattatgacg
    14341 tagtgagacg ttactttgaa taccaaggat ataatgtaga atatgtatca aattttacag
    14401 acgtagatga taaattaatt aaacgttctc aagaattaaa tcagtctgtt cccgaaattg
    14461 cagaaaaata tatcgctgct tttcatgaag atgttggtgc gttaaatgtt agaaaagcga
    14521 cttcaaatcc aagggtaatg gaccatatgg atgacattat tcaatttatt aaagatttgg
    14581 tggatcaagg ttatgcatat gaaagtggtg gcgatgttta ctttagaaca cgtaaatttg
    14641 aaggttatgg taaattaagt catcaatcca tagatgactt aaaagtgggt gctcgtatag
    14701 atgcaggaga gcataaagaa gatgcacttg attttacatt gtggaaaaaa gcgaagcctg
    14761 gcgagattag ttgggatagc ccatttggtg aaggtagacc aggatggcat atagaatgtt
    14821 ctgtaatggc atttcatgag ctaggaccta caattgatat acatgcgggt ggttcagatt
    14881 tacaatttcc acatcatgaa aatgaaatag cacaatcaga agcacataat catgcgccat
    14941 ttgctaatta ttggatgcat aatggtttca ttaatattga taatgaaaaa atgagtaaat
    15001 cactaggcaa ctttatttta gttcacgata ttattaaaga agttgatcca gatgtactaa
    15061 gattctttat gattagcgta cattatagaa gcccaattaa ctataatcta gaattggtag
    15121 aatcagcacg tagtggacta gagcgtattc gcaatagtta tcaattaatt gaagagcgcg
    15181 cacaaattgc tactaatatt gaaaatcaac agacatatat tgatcaaatt gatgcgattt
    15241 taaatcgttt tgaaacagtt atgaatgatg attttaatac agctaatgca attacagctt
    15301 ggtatgattt agcaaaactt gcgaataaat atgtactaga gaatacaaca tcaacagaag
    15361 taattgataa atttaaagca gtttatcaaa ttttcagcga tgttttaggt gtaccgttaa
    15421 aatctaaaaa tgcagatgaa ttattggatg aagatgttga aaaattaatc gaagagcgta
    15481 atgaagcaag gaaaaacaaa gattttgcac gagcagatga gattcgagac atgctgaaat
    15541 cacaaaacat tatattagaa gacacacctc aaggggttag atttaaacgt ggataatcaa
    15601 caagataatc acattaaatt attgaatcca ttgaccttag catatatggg agacgcagtc
    15661 ttagatcaat atgtacgtac ctatatcgtt ttaaagctta aaagtaagcc taataaacta
    15721 catcaaatgt ctaaaaaata tgtatctgcc aaaagtcagg cgcaaacgtt agaatattta
    15781 atggagcaag aatggtttac agacgaagaa atggatattt tgaagcgagg gcgtaacgcg
    15841 aaaagtcata ctaaagctaa aaacactgat gttcaaacat atcgtaaaag ttcagcgata
    15901 gaagcagtga taggttttct ttatttagaa aaaagagaag aacgattaga ggcattatta
    15961 aataaaataa taacaatagt aaacgaaagg tagtgacgat gtggaagata cggttattgt
    16021 tggtaggcat gctgttagag aagcgattat tactgggcat ccgataaata agatattgat
    16081 tcaagaaggt attaaaaagc aacaaattaa tgaaatttta aaaaatgcaa aagatcaaaa
    16141 aatcattgtt caaactgtac caaaatctaa attagatttt ttagcaaatg caccacatca
    16201 gggtgttgca gcgcttattg caccatatga atatgctgac ttcgatcaat ttttaaaaca
    16261 gcaaaaagaa aaagaaggtt tatcgacagt acttatatta gacggcttag aagacccgca
    16321 taacttggga tcaattttaa gaacagccga tgcaacggga gttgatggtg ttattattcc
    16381 taaacgtcgt tctgttacac taacgcaaac agttgcaaaa gcctcaacag gtgcaattga
    16441 acatgtacca gttattcgag tgacaaattt agctaaaact atcgatgaac taaaagataa
    16501 tggcttttgg gtagctggca ctgaagctaa taatgcaaca gattatagaa atctagaagc
    16561 ggacatgtca ttggctattg taattggtag cgaaggacag ggtatgagtc gcctagtaag
    16621 tgataaatgc gatttttata ttaagattcc aatggttgga catgtaaaca gtttgaatgc
    16681 ttcggttgca gcaagtttaa tgatgtacga agtatttcga aaaagacatg atgttggaga
    16741 aatataatga aagaacgtta cttaatcatt gatggataca atatgatagg acaatcacca
    16801 acgctaagcg ccattgcaaa agagaattta gaagaagcta gaatacaatt aatagatgca
    16861 attgcaaatt ataatgcagt tatttcagat gaaattattt gtgttttcga tgcttatgac
    16921 caatcgggtg ttgaaagaga atacatgtat catggcgtta aaacgatttt taccaaggaa
    16981 aaagaaacag ctgatagttt catagaacgt tatgtttatg aactttatga caaacacact
    17041 aagcatatta cagttgtaac aagtgatatg agtgagcaac atgctatctt tggatcaggt
    17101 gcatatagaa tatcatctcg cgaaatgtgg agagatttaa aagaaaatga aattgatgtg
    17161 agtaaatcat tagatgatat aagtgaaaac aagccaagaa ctcgaattcc gttatcttct
    17221 gaaatccttg cagaatttga aaaaatacga agaggacatc ataagaaatg acatttccgt
    17281 catcttgaaa ttttgaatgt aacaatatta aactaacctt aaattttaga tagaaggggt
    17341 tagtttaata tttgaaatat gatttgacaa ctcaagacag tacaatcaaa cgtaacaatg
    17401 caataaatga taaagacttc gaaaagttag taatggatct acaaccatta attattcgac
    17461 gcatcaaaac atttggattt aatcattatg atttagaaga cttatatcaa gaaatactta
    17521 tacggatgta taggtcggtc caaacatttg attttagtgg agagcagcct ttcacaaatt
    17581 atgttcaatg tttaattacg tctgtaaagt atgattattt gagaaaatat ttagctacaa
    17641 ataaaagaat ggataatttg attaatgaat atagagttac gtatccatgt gcaataaagc
    17701 gttttgatgt tgaaaacaat tatttgaata aattagcaat taaagagttg atttgtcagt
    17761 ttaagtattt gagtgcattt gaaaaagatg tcatgtattt aatgtgtgaa caatataagc
    17821 cgagagaaat tgctcaactg atgcatgtaa aagagaaagt gatttataat gccatacaac
    17881 gatgtaaaaa taaaataaaa cgttatttca aaatgatttg aaaagcgcct taggacgtga
    17941 attgaattag aacgtgttac ttactgatgg tttgacattt gttataaatt ttatgtatag
    18001 tatactggta ttataatgaa taaaggtgaa ttattgtgag aaaaatacct ttaaattgtg
    18061 aagcttgtgg caatagaaat tataatgttc ctaagcaaga aggctcggca acaagattaa
    18121 ccttaaagaa atattgtcca aaatgtaacg cgcacacaat tcataaagaa tcgaaataaa
    18181 tacattcgaa ataatacttt gataatatgt tcaaaggatt tggaggttga gcagatggct
    18241 aaaaaagaaa gtttctttaa aggcgttaag tctgaaatgg aaaaaacaag ttggccgacg
    18301 aaagaagagc tatttaaata tactgtaatt gtagtttcta ctgttatatt cttcttagtc
    18361 tttttctatg ccttagattt aggaattaca gcattgaaaa atttattatt tggttagagg
    18421 agtgaagaca tgtctgaaga agttggcgca aagcgttggt atgcagtgca tacatattct
    18481 ggatatgaaa ataaagttaa aaagaattta gaaaaaagag tagaatctat gaatatgact
    18541 gaacaaatct ttagagtagt cataccggaa gaagaagaaa ctcaagtaaa agatggcaaa
    18601 gctaaaacga ctgttaaaaa aacattccct ggatatgttt tagtggaatt aatcatgaca
    18661 gatgaatcat ggtatgtggt aagaaataca ccaggcgtta ctggttttgt aggttctgca
    18721 ggtgcagggt ctaagccaaa tccattgtta ccagaagaag ttcgcttcat cttaaaacaa
    18781 atgggtctta aagaaaagac tatcgatgtt gaactcgaag ttggcgagca agttcgtatt
    18841 aaatcaggtc catttgcgaa tcaagttggt gaagttcaag aaattgaaac agataagttt
    18901 aagctaacag tattagtaga tatgtttggc cgagaaacac cagtagaagt tgaattcgat
    18961 caaatagaaa agctttaatt aacaattaaa gttattaaac taaccaaaag ataaaaaaga
    19021 gtattgattt tttaattaga aaagtgttaa aattatgtgg tcgcgctttt agagcgccca
    19081 tttcgtcacg aaatgttaag agtgggaggg caaaactgag ccctgtgacc acatcacgat
    19141 atcaaggagg tgcacatcgt ggctaaaaaa gtagataaag ttgttaaatt acaaattcct
    19201 gcaggtaaag cgaatccagc accaccagtt ggtccagcat taggtcaagc aggtgtgaac
    19261 atcatgggat tctgtaaaga gttcaatgca cgtactcaag atcaagcagg tttaattatt
    19321 ccggtagaaa tcagtgttta tgaagatcgt tcatttacat ttattacaaa aactccaccg
    19381 gctccagtat tacttaaaaa agcagctggt attgaaaaag gttcaggcga accaaacaaa
    19441 actaaagttg ctacagtaac taaagatcaa gtacgcgaaa ttgctaacag caaaatgcaa
    19501 gacttaaacg ctgctgacga agaagcagct atgcgtatta tcgaaggtac tgcacgtagt
    19561 atgggtatcg ttgtagaata attttacgaa tattaaattt gattacatga tttaaacgat
    19621 gaagcagata acagagataa taatgatgaa ttataaatat aatctgaatg actagattaa
    19681 tgattgattt attcataaga ttaattcttc tgttgtctgc tcttaacttg catatagcaa
    19741 gtaatgtggg aggaaattcc gctaaaacca ctaaaggagg aactataaat ggctaaaaaa
    19801 ggtaaaaagt atcaagaagc agctagtaaa gttgaccgta ctcagcacta cagtgttgaa
    19861 gaagcaatta aattagctaa agaaacaagc attgctaact ttgacgcttc tgttgaagtt
    19921 gcattccgtt taggaattga tacacgtaaa aatgaccaac aaatccgtgg tgcagttgta
    19981 ttaccaaacg gaactggtaa atcacaaagt gtattagtat tcgctaaagg tgacaaaatt
    20041 gctgaagctg aagcagcagg tgctgactat gtaggtgaag cagaatacgt tcaaaaaatc
    20101 caacaaggtt ggttcgactt cgatgtagta gttgctacac cagacatgat gggtgaagtt
    20161 ggtaaattag gtcgtgtatt aggaccaaaa ggtttaatgc caaaccctaa aactggaact
    20221 gtaacaatgg atgttaaaaa agctgttgaa gaaatcaaag ctggtaaagt agaataccgt
    20281 gctgaaaaag ctggtatcgt acatgcatca attggtaaag tttcatttac tgatgaacaa
    20341 ttaattgaaa acttcaatac tttacaagat gtattagcta aagctaaacc atcatctgct
    20401 aaaggtacat acttcaaatc tgttgctgta actacaacaa tgggtcctgg agttaaaatt
    20461 gatactgcaa gtttcaaata ataaatgata taaacaatta caggctgaaa gaaatatctt
    20521 tcagtctgta aaaatatatt gacaataagt aatttccaag ttatattact tattgtgatt
    20581 attttaccta agacagtagg agttatttat aacttaaaat ttatcctgcc gaggctaaaa
    20641 ttgacttgaa cgtgatgatc tatgatcttt caagcacttt ttgccgtagg tagaaagtgc
    20701 tttttttatt aattttaaaa aaagcaccaa aaatttaaat ggaggtgtct gaatgtctgc
    20761 tatcattgaa gctaaaaaac aactagttga tgaaattgct gaggtactat caaattcagt
    20821 ttcaacagta atcgttgact accgtggatt aacagtagct gaagttactg acttacgttc
    20881 acaattacgt gaagctggtg ttgagtataa agtatacaaa aacactatgg tacgtcgtgc
    20941 agctgaaaaa gctggtatcg aaggcttaga tgaattctta acaggtccta ctgctattgc
    21001 aacttcaagt gaagatgctg tagctgcagc gaaagtaatt tctggatttg ctaaagatca
    21061 tgaagcatta gaaattaaat caggcgttat ggaaggcaat gttattacag cagaagaagt
    21121 taaaactgtt ggttcattac cttcacacga tggtcttgta tctatgcttt tatcagtatt
    21181 acaagctcct gtacgcaact tcgcttatgc ggttaaagct attggagaac aaaaagaaga
    21241 aaacgctgaa taatttttag cgtaaaaaaa ttaaaaataa tggaggaatt ataaaatggc
    21301 taatcatgaa caaatcattg aagcgattaa agaaatgtca gtattagaat taaacgactt
    21361 agtaaaagca attgaagaag aatttggtgt aactgcagct gctccagtag cagtagcagg
    21421 tgcagctggt ggcgctgacg ctgcagcaga aaaaactgaa tttgacgttg agttaacttc
    21481 agctggttca tctaaaatca aagttgttaa agctgttaaa gaagcaactg gtttaggatt
    21541 aaaagatgct aaagaattag tagacggagc tcctaaagta atcaaagaag ctttacctaa
    21601 agaagaagct gaaaaactta aagaacaatt agaagaagtt ggagctactg tagaattaaa
    21661 ataattcaag tatcttaaac ttaataatca aagttttata gcaagtattg ctataatata
    21721 atgattcttt gagaagttaa aaccccgtta ttttgataac ggggttttat tcatttaaag
    21781 actgagtgaa atgttataat tataatgacg agttacaaag tgaagatgag gtggaataat
    21841 gagtcattat tacgatgaag atccaagtgt aattagcaat gaacaacgta ttcaatatca
    21901 attaaaccat cataaaattg atttaataac tgataacgga gtgttttcga aagataaagt
    21961 agattatggt tcagatgttc ttgttcaaac ttttttaaaa gcgcatccac ctggttcaag
    22021 taagcgaatt gccgatgttg gttgtggtta cggaccaatt ggtttgatga ttgctaaagt
    22081 atcaccacat cattcaatta caatgctaga tgttaatcac agagcgctag ccttagttga
    22141 aaaaaacaaa aaattaaatg gtattgataa tgtgatcgta aaggaaagtg atgctttgtc
    22201 tgctgtggaa gacaaaagtt ttgattttat tttaaccaat ccaccaataa gagcagggaa
    22261 agaaaccgtg catcgtatat tcgagcaagc attacataga ttagactcga acggtgaact
    22321 attcgttgta attcagaaga agcaaggtat gccatctgca aagaaaagaa tgaatgaact
    22381 ttttggaaat gtagaagtgg taaataaaga taaaggatat tacattctga gaagtataaa
    22441 agcttgaaat gaaatggata ttctgttata gttatataat gtaaaaattt atgttcaata
    22501 agtgtgtact tttacgttaa atagataagt taattaagaa taaatataga atcgaaaatg
    22561 gtgtcatcat tagtgttgcc gttttctttt tgtcttttta ttaatatgct tatggtattt
    22621 agctaaaagc ggatcacata atttttgagg ggtgaatctg tttggcaggt caagttgtcc
    22681 aatatggaag acatcgtaaa cgtagaaact acgcgagaat ttcagaagta ttagaattac
    22741 caaacttaat agaaattcaa actaaatctt acgagtggtt cctaagagaa ggtttaatcg
    22801 aaatgtttag agacatttct ccaattgaag attttactgg taatttgtca ttagagtttg
    22861 tggattaccg tttaggagaa ccaaaatatg atttagaaga atctaaaaac cgtgacgcta
    22921 cttatgctgc acctcttcgt gtaaaagtgc gtctaatcat taaagaaaca ggagaagtta
    22981 aagaacaaga agtctttatg ggtgatttcc cattaatgac tgatacaggt acgttcgtta
    23041 tcaatggtgc agaacgtgta atcgtatctc aattagttcg ttcaccatcc gtttatttca
    23101 atgaaaaaat cgacaaaaat ggtcgtgaaa actatgatgc aacaattatt ccaaaccgtg
    23161 gtgcatggtt agaatatgaa acagatgcta aagatgttgt atacgtacgt attgatagaa
    23221 cacgtaaact accattaaca gtattgttac gtgcattagg tttctcaagc gaccaagaaa
    23281 ttgttgacct tttaggtgac aatgaatatt tacgtaatac tttagagaaa gacggcactg
    23341 aaaacactga acaagcgtta ttagaaatct atgaacgttt acgtccaggt gaaccaccaa
    23401 ctgttgaaaa tgctaaaagt ctattgtatt cacgtttctt tgatccaaaa cgctatgact
    23461 tagcaagcgt gggtcgttat aaaacaaaca aaaaattaca tttaaaacat cgtttattta
    23521 atcaaaaatt agctgagcca attgtaaata ctgaaactgg tgaaattgta gttgaagaag
    23581 gtacagtgct tgatcgtcgt aaaatcgacg aaatcatgga tgtacttgaa tcaaatgcaa
    23641 acagcgaagt gtttgaattg catggtagcg ttatagacga gccagtagaa attcaatcaa
    23701 ttaaagtata tgttcctaac gatgatgaag gtcgtacgac aactgtaatt ggtaatgctt
    23761 tccctgactc agaagttaaa tgcattacac cagcagatat cattgcttca atgagttact
    23821 tctttaactt attaagcggt attggatata cagatgatat tgaccattta ggtaaccgtc
    23881 gtttacgttc tgtaggtgaa ttactacaaa accaattccg tatcggttta tcaagaatgg
    23941 aaagagttgt acgtgaaaga atgtcaattc aagatactga gtctatcaca cctcaacaat
    24001 taattaatat tcgacctgtt attgcatcta ttaaagaatt ctttggtagc tctcaattat
    24061 cacaattcat ggaccaagca aatccattag ctgagttaac gcataaacgt cgtctatcag
    24121 cattaggacc tggtggttta acacgtgaac gtgctcaaat ggaagtacgt gacgttcact
    24181 actctcacta tggccgtatg tgtccaattg aaacacctga gggaccaaac attggattga
    24241 ttaactcatt atcaagttat gcacgtgtaa atgaattcgg ctttattgaa acaccatatc
    24301 gtaaagttga tttagataca catgctatca ctgatcaaat tgactattta acagctgacg
    24361 aagaagatag ctatgttgta gcacaagcaa actctaaatt agatgaaaat ggtcgtttca
    24421 tggatgatga agttgtatgt cgtttccgtg gtaacaacac agttatggct aaagaaaaaa
    24481 tggattatat ggatgtatcg ccgaagcaag ttgtttcagc agcgacagca tgtattccat
    24541 tcttagaaaa tgatgactca aaccgtgcat tgatgggtgc gaacatgcaa cgtcaagcag
    24601 tgcctttgat gaatccagaa gcaccatttg ttggtacagg tatggaacac gttgcagcac
    24661 gtgattctgg tgcagctatt acagctaagc acagaggtcg tgttgaacat gttgaatcta
    24721 atgaaattct tgtacgtcgt ctagttgaag agaacggcgt tgagcatgaa ggtgaattag
    24781 atcgctatcc attagctaaa tttaaacgtt caaactcagg tacatgttac aaccaacgtc
    24841 caatcgttgc agttggagat gttgttgagt ataacgagat tttagcagat ggaccatcta
    24901 tggaattagg agaaatggca ttaggtagaa acgtagtagt tggtttcatg acttgggacg
    24961 gttacaacta tgaggatgcc gttatcatga gtgaaagact tgtgaaagat gacgtgtata
    25021 cttctattca tattgaagag tatgaatcag aagcacgtga tactaagtta ggacctgaag
    25081 aaatcacaag agatattcct aatgtttctg aaagtgcact taagaactta gacgatcgtg
    25141 gtatcgttta tattggtgca gaagtaaaag atggagatat tttagttggt aaagtaacgc
    25201 ctaaaggtgt aactgagtta actgccgaag aaagattgtt acatgcaatc tttggtgaaa
    25261 aagcacgtga agttagagat acttcattac gtgtacctca cggcgctggc ggtatcgttc
    25321 ttgatgtaaa agtattcaat cgcgaagaag gcgacgatac attatcacct ggtgtaaacc
    25381 aattagtacg tgtatacatc gttcaaaaac gtaaaattca tgttggtgat aagatgtgtg
    25441 gtcgacatgg taacaaaggt gtcatttcta agatcgttcc tgaagaagat atgccttact
    25501 taccagacgg acgtccgatc gatatcatgt taaaccctct tggtgtacca tctcgtatga
    25561 acatcggaca agtattagag ctacacttag gtatggctgc taaaaatctt ggtattcacg
    25621 ttgcatcacc tgtatttgac ggtgcaaacg atgacgatgt atggtcaaca attgaagaag
    25681 ctggtatggc tcgtgatggt aaaactgtac tttatgatgg acgtacaggt gaaccattcg
    25741 ataaccgtat ttcagtaggt gtaatgtaca tgttgaaact tgcgcacatg gttgatgata
    25801 aattacatgc gcgttcaaca ggaccatatt cacttgttac acaacaacca cttggcggta
    25861 aagcgcaatt cggtggacaa cgtttcggtg agatggaggt atgggcactt gaagcatatg
    25921 gtgctgcata cacattacaa gaaatcttaa cttacaaatc cgatgataca gtaggacgtg
    25981 tgaaaacata cgaggctatt gttaaaggtg aaaacatctc tagaccaagt gttccagaat
    26041 cattccgagt attgatgaaa gaattacaaa gtttaggttt agatgtaaaa gttatggatg
    26101 agcaagataa tgaaatcgaa atgacagacg ttgatgacga tgatgttgta gaacgcaaag
    26161 tagatttaca acaaaatgat gctcctgaaa cacaaaaaga agttactgat taatacgcaa
    26221 tttacaaaac aggcaaaaag atactaagct gaattttatt gatgattcag tttagtactt
    26281 taagctattt taaataaatg caaatcaatc aaatagcaca gctaatctaa attgaaggag
    26341 gtaggctcct tgattgatgt aaataatttc cattatatga aaataggatt ggcttcacct
    26401 gaaaaaattc gttcttggtc ttttggtgaa gttaaaaaac ctgaaacaat caactaccgt
    26461 acattaaaac ctgaaaaaga tggtctattc tgtgaaagaa ttttcggacc tacaaaagac
    26521 tgggaatgta gttgtggtaa atacaaacgt gttcgctaca aaggcatggt ctgtgacaga
    26581 tgtggagttg aagtaactaa atctaaagta cgtcgtgaaa gaatgggtca cattgaactt
    26641 gctgctccag tttctcacat ttggtatttc aaaggtatac caagccgtat gggattatta
    26701 cttgacatgt caccaagagc attagaagaa gttatttact ttgcttctta tgttgttgta
    26761 gatccaggtc caactggttt agaaaagaaa actttattat ctgaagctga attcagagat
    26821 tattatgata aatacccagg tcaattcgtt gcaaaaatgg gtgcagaagg tattaaagat
    26881 ttacttgaag agattgatct tgacgaagaa cttaaattgt tacgcgatga gttggaatca
    26941 gctactggtc aaagacttac tcgtgcaatt aaacgtttag aagttgttga atcattccgt
    27001 aattcaggta acaaaccttc atggatgatt ttagatgtac ttccaatcat cccaccagaa
    27061 attcgtccaa tggttcaatt agatggtgga cgatttgcaa caagtgactt aaacgattta
    27121 taccgtcgtg taattaatcg aaataatcgt ttgaaacgtt tattagattt aggtgcacct
    27181 ggtatcatcg ttcaaaacga aaaacgtatg ttacaagaag ccgttgacgc tttaattgat
    27241 aatggtcgtc gtggtcgtcc agttactggc ccaggtaacc gtccattaaa atctttatct
    27301 catatgttaa aaggtaaaca aggtcgtttc cgtcaaaact tacttggtaa acgtgttgac
    27361 tattcaggac gttcagttat tgcagtaggt ccgagcttga aaatgtacca atgtggttta
    27421 ccaaaagaaa tggcacttga actatttaaa ccattcgtaa tgaaagaatt agttcaacgt
    27481 gaaattgcaa ctaacattaa aaatgcgaag agtaaaatcg aacgcatgga tgatgaagtt
    27541 tgggacgtat tggaagaagt aattagagaa catcctgtat tacttaaccg tgcaccaaca
    27601 cttcatagac ttggtattca agcatttgaa ccaactttag ttgaaggtcg tgcgattcgt
    27661 ctacatccac ttgtaacaac agcttataac gctgactttg acggtgacca aatggcggtt
    27721 cacgttcctt tatcaaaaga ggcacaagct gaagcaagaa tgttgatgtt agcagcacaa
    27781 aacatcttga accctaaaga tggtaaacct gtagttacac catcacaaga tatggtactt
    27841 ggtaactatt accttacttt agaaagaaaa gatgcagtaa atacaggcgc aatctttaat
    27901 aatacaaatg aagtattaaa agcatatgca aacggctttg tacatttaca cactagaatt
    27961 ggtgtacatg caagttcatt caataatcca acatttactg aagaacaaaa caaaaagatt
    28021 cttgctacgt cagtaggtaa aattatattc aatgaaatca ttccagattc atttgcttat
    28081 attaatgaac ctacgcaaga aaacttagaa agaaagacac caaacagata tttcatcgat
    28141 cctacaactt taggtgaagg tggattaaaa gaatactttg aaaatgaaga attaattgaa
    28201 cctttcaaca aaaaattctt aggtaatatt attgcagaag tattcaacag atttagcatc
    28261 actgatacat caatgatgtt agaccgtatg aaagacttag gattcaaatt ctcatctaaa
    28321 gctggtatta cagtaggtgt tgctgatatc gtagtattac ctgataagca acaaatactt
    28381 gatgagcatg aaaaattagt cgacagaatt acaaaacaat tcaaccgtgg tttaatcact
    28441 gaagaagaaa gatataatgc agttgttgaa atttggacag atgcaaaaga tcaaattcaa
    28501 ggtgaattga tgcaatcact tgataaaact aacccaatct tcatgatgag tgattcaggt
    28561 gcccgtggta acgcatctaa ctttacacag ttagcaggta tgcgtggatt gatggccgca
    28621 ccatctggta agattatcga attaccaatc acatcttcat tccgtgaagg tttaacagta
    28681 cttgaatact tcatctcaac tcacggtgca cgtaaaggtc ttgccgatac agcacttaaa
    28741 acagctgact caggatatct tactcgtcgt cttgttgacg tggcacaaga tgttattgtt
    28801 cgtgaagaag actgtggtac tgatagaggt ttattagttt ctgatattaa agaaggtaca
    28861 gaaatgattg aaccatttat cgaacgtatt gaaggtcgtt attctaaaga aacaattcgt
    28921 catcctgaaa ctgatgaaat aatcattcgt cctgatgaat taattacacc tgaaattgct
    28981 aagaaaatta cagatgctgg tattgaacaa atgtatattc gctcagcatt tacttgtaac
    29041 gcacgacatg gtgtttgtga aaaatgttac ggtaaaaacc ttgctactgg tgaaaaagtt
    29101 gaagttggtg aagcagttgg tacaattgca gcccaatcta tcggtgaacc aggtacacag
    29161 cttacaatgc gtacattcca tacaggtggg gtagcaggta gcgatatcac acaaggtctt
    29221 cctcgtattc aagagatttt cgaagcacgt aaccctaaag gtcaagcggt aattacggaa
    29281 atcgaaggtg tcgtagaaga tattaaatta gcaaaagata gacaacaaga aattgttgtt
    29341 aaaggtgcta atgaaacaag atcatacctt gcttcaggta cttcaagaat tattgtagaa
    29401 atcggtcaac cagttcaacg tggtgaagta ttaactgaag gttctattga acctaagaat
    29461 tacttatctg ttgctggatt aaacgcgact gaaagctact tattaaaaga agtacaaaaa
    29521 gtttaccgta tgcaaggtgt agaaatcgac gataaacacg ttgaggttat ggttcgacaa
    29581 atgttacgta aagttagaat tatcgaagca ggtgatacga agttattacc aggttcatta
    29641 gttgatattc ataactttac agatgcaaat agagaagcat ttaaacaccg taagcgtcct
    29701 gcaacagcta aaccagtatt acttggtatt actaaagcat cacttgaaac agaaagtttc
    29761 ttatctgcag catcattcca agaaacaaca agagttctta cagatgcagc aattaaaggt
    29821 aagcgtgatg acttattagg tcttaaagaa aacgtaatta ttggtaagtt aattccagct
    29881 ggtactggta tgagacgtta tagcgacgta aaatacgaaa aaacagctaa accagttgca
    29941 gaagttgaat ctcaaactga agtaacggaa taacaagtat ataacagagg ctaaggcttt
    30001 agcctcttgt tatttttatg taaattattt gatttaatgt tgacgaattc tcttgttcaa
    30061 tgttaatata ttaaaggttg atgcaagcag aactttggag gataaattat tgtctaagga
    30121 aaaagttgca cgctttaaca aacaacattt tgtagttggt cttaaagaaa cgcttaaagc
    30181 gttaaagaaa gatcaagtta catctttgat tattgctgaa gacgttgaag tatatttaat
    30241 gactcgcgtg ttaagccaaa tcaatcagaa aaatatacct gtatcttttt tcaaaagcaa
    30301 acatgctttg ggtaaacatg taggtattaa cgtcaatgcg acaatagtag cattgattaa
    30361 atgagaatta gtaagtgttt tacttactaa attttattta acctaaaaat gaaccacctg
    30421 gatgtgtggg attaaaaagt gaagagagga ggacatatca catgccaact attaaccaat
    30481 tagtacgtaa accaagacaa agcaaaatca aaaaatcaga ttctccagct ttaaataaag
    30541 gtttcaacag taaaaagaaa aaatttactg acttaaactc accacaaaaa cgtggtgtat
    30601 gtactcgtgt aggtacaatg acacctaaaa aacctaactc agcgttacgt aaatatgcac
    30661 gtgtgcgttt atcaaacaac atcgaaatta acgcatacat ccctggtatc ggccataact
    30721 tacaagaaca cagtgttgta cttgtacgtg gtggacgtgt aaaagactta ccaggtgtgc
    30781 gttaccatat tgtacgtgga gcacttgata cttcaggtgt tgacggacgt agacaaggtc
    30841 gttcattata cggaactaag aaacctaaaa actaaggatt tagtttttaa ttaaatctta
    30901 aacttaaaat atttaatata aggaagggag gatttacatt atgcctcgta aaggatcagt
    30961 acctaaaaga gacgtattac cagatccaat tcataactct aagttagtaa ctaaattaat
    31021 taacaaaatt atgttagatg gtaaacgtgg aacagcacaa agaattcttt attcagcatt
    31081 cgacctagtt gaacaacgca gtggtcgtga tgcattagaa gtattcgaag aagcaatcaa
    31141 caacattatg ccagtattag aagttaaagc tcgtcgtgta ggtggttcta actatcaagt
    31201 accagtagaa gttcgtccag agcgtcgtac tactttaggt ttacgttggt tagttaacta
    31261 tgcacgtctt cgtggtgaaa aaacgatgga agatcgttta gctaacgaaa ttttagatgc
    31321 agcaaataat acaggtggtg ccgttaagaa acgtgaggac actcacaaaa tggctgaagc
    31381 aaacaaagca tttgctcact accgttggta agataaaagc ttttaccctg agtgtgttct
    31441 atattaatga attttcatta agcgttcatg cttagggcat cgccatatct atcgtattta
    31501 ttcagtaata taaactggaa ggagaaaaaa tacatggcta gagaattttc attagaaaaa
    31561 actcgtaata tcggtatcat ggctcacatt gatgctggta aaacgactac gactgaacgt
    31621 attctttatt acactggccg tatccacaaa attggtgaaa cacacgaagg tgcttcacaa
    31681 atggactgga tggagcaaga acaagaccgt ggtattacta tcacatctgc tgcaacaaca
    31741 gcagcttggg aaggtcaccg tgtaaacatt atcgatacac ctggacacgt agacttcact
    31801 gtagaagttg aacgttcatt acgtgtactt gacggagcag ttacagtact tgatgcacaa
    31861 tcaggtgttg aacctcaaac tgaaacagtt tggcgtcagg ctacaactta tggtgttcca
    31921 cgtatcgtat ttgtaaacaa aatggacaaa ttaggtgcta acttcgaata ctctgtaagt
    31981 acattacatg atcgtttaca agctaacgct gctccaatcc aattaccaat tggtgcggaa
    32041 gacgaattcg aagcaatcat tgacttagtt gaaatgaaat gtttcaaata tacaaatgat
    32101 ttaggtactg aaattgaaga aattgaaatt cctgaagacc acttagatag agctgaagaa
    32161 gctcgtgcta gcttaatcga agcagttgca gaaactagcg acgaattaat ggaaaaatat
    32221 cttggtgacg aagaaatttc agtttctgaa ttaaaagaag ctatccgcca agctactact
    32281 aacgtagaat tctacccagt actttgtggt acagctttca aaaacaaagg tgttcaatta
    32341 atgcttgacg ctgtaattga ttacttacct tcaccactag acgttaaacc aattattggt
    32401 caccgtgcta gcaaccctga agaagaagta atcgcgaaag cagacgattc agctgaattc
    32461 gctgcattag cgttcaaagt tatgactgac ccttatgttg gtaaattgac attcttccgt
    32521 gtgtattcag gtacaatgac atctggttca tacgttaaga actctactaa aggtaaacgt
    32581 gaacgtgtag gtcgtttatt acaaatgcac gctaactcac gtcaagaaat cgatactgta
    32641 tactctggag atatcgctgc tgcggtaggt cttaaagata caggtactgg tgatacttta
    32701 tgtggtgaga aaaatgacat tatcttggaa tcaatggaat tcccagagcc agttattcac
    32761 ttatcagtag agccaaaatc taaagctgac caagataaaa tgactcaagc tttagttaaa
    32821 ttacaagaag aagacccaac attccatgca cacactgacg aagaaactgg acaagttatc
    32881 atcggtggta tgggtgagct tcacttagac atcttagtag accgtatgaa gaaagaattc
    32941 aacgttgaat gtaacgtagg tgctccaatg gtttcatatc gtgaaacatt caaatcatct
    33001 gcacaagttc aaggtaaatt ctctcgtcaa tctggtggtc gtggtcaata cggtgatgtt
    33061 cacatcgaat tcacaccaaa cgaaacaggc gcaggtttcg aattcgaaaa cgctatcgtt
    33121 ggtggtgtag ttcctcgtga atacattcca tcagtagaag ctggtcttaa agatgctatg
    33181 gaaaatggtg tcttagcagg ttatccttta attgatgtta aagctaaatt atatgatggt
    33241 tcataccatg atgtcgattc atctgaaatg gccttcaaaa ttgctgcatc attagcactt
    33301 aaagaagctg ctaaaaaatg tgatcctgta atcttagaac caatgatgaa agtaactatt
    33361 gaaatgcctg aagagtacat gggtgatatc atgggtgacg taacatctcg tcgtggacgt
    33421 gttgatggta tggaacctcg tggtaatgca caagttgtta atgcttatgt accactttca
    33481 gaaatgttcg gttatgcaac atcattacgt tcaaacactc aaggtcgcgg tacttacact
    33541 atgtacttcg atcactatgc tgaagttcca aaatcaatcg ctgaagatat tatcaagaaa
    33601 aataaaggtg aataatataa cttgttttga ctagctagcc taggttaaaa tacaaggtga
    33661 gcttaaatgt aagctatcat ctttatagtt tgattttttg gggtgaatgc attataaaag
    33721 aattgtaaaa ttctttttgc atcgctataa ataatttctc atgatggtga gaaactatca
    33781 tgagagataa atttaaatat tatttttaat tagaatagga gagattttat aatggcaaaa
    33841 gaaaaattcg atcgttctaa agaacatgcc aatatcggta ctatcggtca cgttgaccat
    33901 ggtaaaacaa cattaacagc agcaatcgct actgtattag caaaaaatgg tgactcagtt
    33961 gcacaatcat atgacatgat tgacaacgct ccagaagaaa aagaacgtgg tatcacaatc
    34021 aatacttctc acattgagta ccaaactgac aaacgtcact acgctcacgt tgactgccca
    34081 ggacacgctg actacgttaa aaacatgatc actggtgctg ctcaaatgga cggcggtatc
    34141 ttagtagtat ctgctgctga cggtccaatg ccacaaactc gtgaacacat tcttttatca
    34201 cgtaacgttg gtgtaccagc attagtagta ttcttaaaca aagttgacat ggttgacgat
    34261 gaagaattat tagaattagt agaaatggaa gttcgtgact tattaagcga atatgacttc
    34321 ccaggtgacg atgtacctgt aatcgctggt tcagcattaa aagctttaga aggcgatgct
    34381 caatacgaag aaaaaatctt agaattaatg gaagctgtag atacttacat tccaactcca
    34441 gaacgtgatt ctgacaaacc attcatgatg ccagttgagg acgtattctc aatcactggt
    34501 cgtggtactg ttgctacagg ccgtgttgaa cgtggtcaaa tcaaagttgg tgaagaagtt
    34561 gaaatcatcg gtttacatga cacatctaaa acaactgtta caggtgttga aatgttccgt
    34621 aaattattag actacgctga agctggtgac aacattggtg cattattacg tggtgttgct
    34681 cgtgaagacg tacaacgtgg tcaagtatta gctgctcctg gttcaattac accacatact
    34741 gaattcaaag cagaagtata cgtattatca aaagacgaag gtggacgtca cactccattc
    34801 ttctcaaact atcgtccaca attctatttc cgtactactg acgtaactgg tgttgttcac
    34861 ttaccagaag gtactgaaat ggtaatgcct ggtgataacg ttgaaatgac agtagaatta
    34921 atcgctccaa tcgcgattga agacggtact cgtttctcaa tccgtgaagg tggacgtact
    34981 gtaggatcag gcgttgttac tgaaatcatt aaataatttc taatttctta gattttatat
    35041 aaaaagaaga tccctcaatc gaggggtctt tttttaatgt gtaaattttg taatggctat
    35101 tcgatttaga agaacaataa ttgatgaaag actgactaat aaaacttata actgataata
    35161 ctgtttaaat aaaattgttg agtcttggac attgtaaaat gctcccttca aagttttcat
    35221 tttttcaatg tctactttga agggagcatt tcattagttt atgtctcaga ttcatatctt
    35281 tcaattaatt taaatgctta atttgtttta aatacttgct ctaattcaat gattttaaaa
    35341 aatacagcta cagcgtattt taatgatttt tcatcaatat caaatttggg attatggtgt
    35401 ggcgctgtaa tacctttact ttcattacca caaccagtca gaaagaatgc acctggtcgt
    35461 actttcaaat aatgtgaaaa atcttctcca atcatcatta aatctgattc attaaagcgt
    35521 acatgtaagt catttgttgc ttctttaata acttgatatg ctttctcgtt attatggaca
    35581 ggcaaatacc ctttaatata attcaaatca tagttaatat catttgctat tgctaaacct
    35641 tgtagaagct tatccatttt gtccattaca tgattctgta tatctgaatc gaaagttcta
    35701 actgtacctt tacaaaatgc ttgatcagga ataacgctat ctgtggtgcc tgcttgaatc
    35761 attccaaatg aaagtacagc ttgtttaact ggatcaatcg tacgtgaaat tattttttgt
    35821 gcacttaaaa tgaactctgc catgattact attgggtcaa tggtttcatg aggtttggca
    35881 ccatgaccgc cacgaccttt aattgtgaca ctaaattcat ctggagaggc catgattgcc
    35941 cccgcacgtg aatgaatagt tccagtagga taaccactcc ataaatgtgt accgtaaatt
    36001 ctatctacat tttccagaca tccagcatct atcatttctt gagaaccacc tggcatgatt
    36061 tcttcaccgt actggaatat taatacaaca ttaccttcta ataaatgttt atgttcatct
    36121 aaaatctctg ctacagtaag taaaattgct gtatgaccat catgcccaca cgcatgcata
    36181 catcctggat ttttagactt ataaggcaca tcgtttaatt cctcgacagg taacgcatca
    36241 aagtcagctc ttaatgcaat ggtaggtcct gtgcccaagc ctttaaatgt ggctttgata
    36301 ccattgcggc cgataggagt ttcaatatca caagataact ggcttaattg gttaacaata
    36361 taatcatgtg tttgaaattc ttcaaaagat aactcagggt attggtgtaa ataacgtctg
    36421 agttgaattg ttttattttc tttattattt gctagttgga accaatctaa cacccttatc
    36481 actactttct aaaataatgt ttatagtata acattttatg aaattatcgt actaaatgat
    36541 tgctttgaga tattttatct atgaatgata aggctttcaa gttatgtaga attactgtat
    36601 gataaaggta ttaccaaaca atacttaagg gggattatat actgtggttc aatcattaca
    36661 tgagttttta gaggaaaata taaattatct aaaagaaaat ggtttgtata atgaaataga
    36721 tacaattgaa ggtgcaaacg gaccagaaat caaaatcaat gggaaatcat acattaactt
    36781 atcttcaaat aattatttag gactagcaac aaatgaagat ttgaaatcag ctgcaaaagc
    36841 agctattgat acacatggag taggtgcagg cgctgttcgt acaatcaatg gtacattaga
    36901 tttacacgac gaattagaag aaacactagc aaaatttaaa ggaacagaag ctgcaatagc
    36961 ttatcaatca ggatttaatt gtaatatggc tgctatttca gctgtcatga ataaaaatga
    37021 tgctatttta tcagatgagc ttaatcatgc atcaattatt gatggatgtc gcttatctaa
    37081 agctaaaatt attcgagtta accattcaga catggatgat ttacgtgcga aagcaaaaga
    37141 agcagttgaa tcaggtcaat acaataaagt gatgtatatc actgatggcg tttttagtat
    37201 ggatggtgat gtggctaaat tacctgaaat tgtagaaatt gcagaagaat ttggtttatt
    37261 aacttatgta gacgacgctc atggttcagg tgttatgggt aaaggcgctg gtacggttaa
    37321 acattttggt ttacaagata aaatcgattt ccaaataggt acgctttcta aagcaattgg
    37381 tgtcgttggc ggttatgtag caggtacaaa agagttaata gattggttaa aagcacaatc
    37441 acgaccattc ttattctcta catcattagc acctggggat accaaagcaa taactgaagc
    37501 agttaaaaag ttaatggatt caactgaatt acatgataaa ttatgggaca atgcacaata
    37561 tttaaaaaat ggattgtcaa aattaggata tgatacaggt gagtcagaaa ctccaattac
    37621 accagtaatt attggtgatg aaaaaacaac tcaagaattt agtaagcgtt taaaagacga
    37681 aggtgtctat gtgaaatcta tcgttttccc aacagtacca agaggtacag gacgtgtaag
    37741 aaatatgcct acagctgcac atacaaaaga catgttagat gaagcaattg cggcttatga
    37801 aaaagtagga aaagaaatga agttgattta atatttattt attcccacgg caaatattgt
    37861 cgtgggcttt tttttaaatg tttagtttat taacagtaag ttcttataac aatgtttagt
    37921 gctccccaaa attaaagttt taattttaaa agcatcttgt aaaatttagt tgtatttttt
    37981 tcaaagaaat tcattttgat tatttttgat aatgagcatt ttaatagtaa tacatgttta
    38041 tagtgtgtag tatatgtcta tactagtagt aactatatag agaaagtagg aataaactat
    38101 gtcacaagat gtaaatgaat taagtaagca accaacgcca gataaagcag aagataacgc
    38161 attttttcca tcaccatatt cccttagtca atatacagca cctaaaacag attttgatgg
    38221 tgttgaacac aaaggtgcct ataaagatgg taaatggaaa gtattgatga ttgctgctga
    38281 agagagatat gtattattgg aaaatggaaa aatgttctct acgggtaatc atcctgttga
    38341 aatgttatta cctttacatc atttaatgga agcaggtttt gacgttgatg ttgcgacatt
    38401 atctggttat ccagttaaat tagaattatg ggctatgcca actgaagacg aggcagttat
    38461 aagtacttat aataaattga aagaaaaatt aaaacagcca aaaaaattag cagatgtgat
    38521 taaaaatgaa ttaggacctg attcagatta tttatctgtc tttatcccag gcggacatgc
    38581 tgcagttgtt ggtatttctg aaagtgagga tgttcaacaa acattagatt gggcattaga
    38641 caatgaccgc tttatagtta cattatgtca tggaccagca gcactacttt cagcagggct
    38701 taacagagaa aaatctccat tagaaggata ctctgtttgt gtcttccctg actcattaga
    38761 tgaaggtgca aatattgaaa taggttattt acctggacgc ttgaaatggt tagttgctga
    38821 tttattaact aaacaaggat taaaagtagt taacgaggat atgacaggaa gaacgttaaa
    38881 agatcgtaaa ttattaacag gtgacagtcc tttagcttca aatgagttag gaaaattagc
    38941 agttaatgaa atgttaaatg caatacaaaa taaataatta aatattaatt agaggagcct
    39001 catatgtaaa tgtatgaggg ctcttttttt tggcaaaatt taagtgatac ttgtaaaata
    39061 gaacctatta tgagtatgat ttaagaaaac gcttgcaaaa ctaataaccg caactagcga
    39121 tatggaggaa acatgatgtc ttatagcatt ggaattgatt atggaacagc ttcaggccgt
    39181 gtgtttttaa ttaatacaac taacggtcaa gtagtatcaa aatttgtgaa accatataca
    39241 catggtgtca ttgaaagtga attaaatggt ttgaaaatac cacatacata tgcacttcaa
    39301 aatagtaatg attatttaga aattatggaa gaaggaatat catatatagt acgtgaatca
    39361 aaaatagatc cagacaatat agtaggtatt ggtatagact ttacttcatc tactattatt
    39421 tttactgacg aaaatcttaa cccggtacat aatttaaaac aatttaaaaa caatccacat
    39481 gcgtatgtga aactttggaa acatcatggt gcatataaag aagcagagaa attatatcaa
    39541 actgctattg aaaataataa taagtggtta ggccattatg gatataatgt tagtagtgaa
    39601 tggatgattc ccaaaataat ggaagtcatg aatcgagcac cagaaattat ggaaaaaacg
    39661 gcttatatta tggaagcggg cgattggatt gtaaataaat taactaataa aaatatacgc
    39721 tcgaattgtg gattaggttt caaagcattt tgggaagaag aaacagggtt tcattatgat
    39781 ttatttgata aaatagaccc caaattatca aaagtaattc aagataaagt atctgcaccg
    39841 gttgttaata ttggtgaagc agtagggaaa ctggatgata aaatggcaca gaaattagga
    39901 ttatcaaaag agactatggt aagtcctttt attattgatg cccatgctag tttattaggt
    39961 attgggtctg aaaaagataa agaaatgact atggtgatgg gaacaagcac atgccatctt
    40021 atgttaaatg aaaagcaaca tcaagtgcca ggtatatcag gttctgtaaa aggagcaatt
    40081 attccagaat tatttgctta tgaagcgggg caatcagcag taggtgattt gtttgagtat
    40141 gtcgctaagc aagcaccaaa gtcatatgta gatgaagcag caaatagaaa tatgactgta
    40201 tttgaattaa tgaatgaaaa gataaaacat caaatgccag gtgaaagtgg gctcattgct
    40261 cttgattggc ataatggaaa tcgaagtgta ttaagtgata gcaatttaac aggttgtatc
    40321 tttggattaa ctttacaaac taagcatgaa gatatttata gagcatattt agaagctaca
    40381 gcatttggta ctaagatgat tatgcaacag tatcaagatt ggcatatgga agtagaaaag
    40441 gtatttgcat gtggcggtat acctaaaaag aatgctgtta tgatggatat ctatgcgaat
    40501 gtactgaata aaaaactaat tgttatggat agtgagtatg caccagcaat aggcgcagca
    40561 atattaggtg cagtcagtgg tggcgcacat aattcaatta atgatgcagt tgatgctatg
    40621 aaagagccaa ttttatacga aattaatcca gaagcggaaa aagtacaaag gtatgaaaca
    40681 ttatttaaag cttataaggc tttacatgat atccatggtt ataaaaaagc taatataatg
    40741 aaagatatcc agagtttaag agttgaggga taaaaaattt aatttgacag taattagcaa
    40801 taataaacgc tataaattac taaaatcaat taatacaact agaatttcca tttacagaaa
    40861 tatcagcaac atgtacatgt catatataca agaaagcgct tttatattat aattattatg
    40921 aaaatgaata ttatgtccgc ttgtgatgga ctatagctat atagaagaga caaaggagat
    40981 atttctatga aaaaaattat gattactggt gcattaggac aaattggtac agaattagtt
    41041 gttaagtgca gagaaattta tgggacagat aatgttcttg ctacagatat tagggaacct
    41101 gaagcagact cacctgtaca aaatggacca tttgaaatct tagacgtaac agatcgtgac
    41161 cgtatgtttg agttagttag ggactttgaa gcggatagtc taatgcatat ggcagcatta
    41221 ttatcagcaa ctgctgagaa aaatccaatt ctagcttggg atttaaatat gggtggatta
    41281 atgaatgcat tagaagctgc aagaacttat aatttgcact ttttcacacc aagttcaatt
    41341 ggtgcatttg gagactcaac tcctaaagtt aatacgccac aagtaacgat tcagcaacct
    41401 acgacaatgt atggtgtaaa taaagtagct ggagaattat tgtgtcaata ctatttcaaa
    41461 cgttttggtg tagatacaag aagtgttaga ttcccaggtt taatctcgca tgttaaagag
    41521 ccaggtggcg gtactacaga ctatgctgtt gaaatatact tcaaagcagt aagagagggt
    41581 cattatacaa gcttcataga taaaggcacg tatatggata tgatgtatat ggatgatgca
    41641 attgaagcaa ttattaaact tatggaagca gacgacgcta aattagaaac tagaaatggt
    41701 tataatttga gcgcaatgag ttttgatcca gagatggtaa aagaagcaat tcaagaatac
    41761 tatcccaatt ttacattaga ttacgatgtt gatcctatta gacaaggtat cgctaatagt
    41821 tggccggatt ctattgatac aagctgttca cgtggcgaat ggggatttga tcctaaatat
    41881 gatttagcga gcatgactaa attaatgtta gaagctattg aacaaaaaga tactgttaaa
    41941 aataataact aatcatttcc attcacttta atacacggaa tgatatttta aattactctt
    42001 tattt
//
LOCUS       DAHHYJ010000008        38110 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000008 DAHHYJ010000000
VERSION     DAHHYJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 38110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.008"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            74..349
                     /locus_tag="NBK22_000332"
     CDS             74..349
                     /locus_tag="NBK22_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413549.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(535..1257)
                     /locus_tag="NBK22_000333"
     CDS             complement(535..1257)
                     /locus_tag="NBK22_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCD2413550.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            1611..2060
                     /locus_tag="NBK22_000334"
     CDS             1611..2060
                     /locus_tag="NBK22_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCD2413551.1"
                     /translation="MTALFPYIAFENSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPFADQFWGGKMGVFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(2141..2728)
                     /locus_tag="NBK22_000335"
     CDS             complement(2141..2728)
                     /locus_tag="NBK22_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2413552.1"
                     /translation="MIIDRRIRKSQVAMRNAFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLEDQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMVEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIMHFNTIVNQGPATIIKNIQ"
     gene            2871..4553
                     /locus_tag="NBK22_000336"
     CDS             2871..4553
                     /locus_tag="NBK22_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCD2413553.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAAKQPWSN
                     GNIGTNSVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWI
                     QGIFARWTDNPNIEDMIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLFLNAENHTLNHAKISSAHVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(4662..5297)
                     /locus_tag="NBK22_000337"
     CDS             complement(4662..5297)
                     /locus_tag="NBK22_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCD2413554.1"
                     /translation="MKKVISLFVAILSVLIFTDNANAENNKPEGVNENSNLIPVRQPD
                     ANYPGPVSDIARVKKLDNLIDGTKDPDVIKQLKDDNEVQNNAVKAKKSGIVSDCRHLL
                     KHPDNLMDGTKDPKVIKQLKNANVTSNNGAVNPSVKANDTHTKSNKQMAKQQSIHLDS
                     KSKSGHKQQLKDLPKTGVNNESNTAIMVLFSGLILLVVSLVTRFIKSNSHQ"
     gene            complement(5634..6017)
                     /locus_tag="NBK22_000338"
     CDS             complement(5634..6017)
                     /locus_tag="NBK22_000338"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCD2413555.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(6019..6870)
                     /locus_tag="NBK22_000339"
     CDS             complement(6019..6870)
                     /locus_tag="NBK22_000339"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCD2413556.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEKERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(6863..7681)
                     /gene="panB"
                     /locus_tag="NBK22_000340"
     CDS             complement(6863..7681)
                     /gene="panB"
                     /locus_tag="NBK22_000340"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCD2413557.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            7754..8614
                     /locus_tag="NBK22_000341"
     CDS             7754..8614
                     /locus_tag="NBK22_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD2413558.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HIPFKNVCQAVVYISGQKKGDVVTHFRDYQLRIKDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLDFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(8700..9404)
                     /gene="budA"
                     /locus_tag="NBK22_000342"
     CDS             complement(8700..9404)
                     /gene="budA"
                     /locus_tag="NBK22_000342"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD2413559.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFSLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGTIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
     gene            complement(9683..10642)
                     /locus_tag="NBK22_000343"
     CDS             complement(9683..10642)
                     /locus_tag="NBK22_000343"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD2413560.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
     gene            11329..12777
                     /locus_tag="NBK22_000344"
     CDS             11329..12777
                     /locus_tag="NBK22_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2413561.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAEHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            complement(12857..14194)
                     /locus_tag="NBK22_000345"
     CDS             complement(12857..14194)
                     /locus_tag="NBK22_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCD2413562.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLAIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKFKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            14452..14868
                     /locus_tag="NBK22_000346"
     CDS             14452..14868
                     /locus_tag="NBK22_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413563.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFVFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLIALVIPRIK"
     gene            14991..15881
                     /locus_tag="NBK22_000347"
     CDS             14991..15881
                     /locus_tag="NBK22_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCD2413564.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNDELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(16075..17571)
                     /gene="mqo"
                     /locus_tag="NBK22_000348"
     CDS             complement(16075..17571)
                     /gene="mqo"
                     /locus_tag="NBK22_000348"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCD2413565.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            17867..18196
                     /locus_tag="NBK22_000349"
     CDS             17867..18196
                     /locus_tag="NBK22_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413566.1"
                     /translation="MDYSILNLYSILIQYKCKINHKKEIQYLHPRKTSQLPLILKTIL
                     NLSLIGIVIIVFTHAEFKFNAGDSNVFLLTWDIRGLTSYIFSFLILVALVSKLILIFI
                     TRRQRTS"
     gene            complement(18256..19854)
                     /locus_tag="NBK22_000350"
     CDS             complement(18256..19854)
                     /locus_tag="NBK22_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCD2413567.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKEYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDMAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTKGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDSEIEKWQQQKDSNQ"
     gene            complement(20048..20491)
                     /locus_tag="NBK22_000351"
     CDS             complement(20048..20491)
                     /locus_tag="NBK22_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCD2413568.1"
                     /translation="MNIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRQ
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            complement(20771..20983)
                     /locus_tag="NBK22_000352"
     CDS             complement(20771..20983)
                     /locus_tag="NBK22_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD2413569.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            complement(21261..22970)
                     /gene="betA"
                     /locus_tag="NBK22_000353"
     CDS             complement(21261..22970)
                     /gene="betA"
                     /locus_tag="NBK22_000353"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCD2413570.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHFEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRTGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGSEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            complement(23231..24721)
                     /gene="betB"
                     /locus_tag="NBK22_000354"
     CDS             complement(23231..24721)
                     /gene="betB"
                     /locus_tag="NBK22_000354"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCD2413571.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            24790..24885
                     /locus_tag="NBK22_000355"
     CDS             24790..24885
                     /locus_tag="NBK22_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413572.1"
                     /translation="MKNVSIYKTTQELSFVSLLDFCLTKHLTLML"
     gene            24974..25537
                     /locus_tag="NBK22_000356"
     CDS             24974..25537
                     /locus_tag="NBK22_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCD2413573.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(25725..26411)
                     /locus_tag="NBK22_000357"
     CDS             complement(25725..26411)
                     /locus_tag="NBK22_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD2413574.1"
                     /translation="MTNQFKNKQSKLHDSLESITKNLYATPTSELPFDNRFLFKSFIL
                     KRETGNIVIYHSGHLGDSQQNIASLGGVSKVLMNHDHESIGGSNQVEAPYFIHENDVA
                     ALKHKISVQKQFSNRVMLDKDLEVIPAPGHTPGTTLFLWDDGHHRYLFTGDFICFEGK
                     RWRTVILGSSDREKSIQSLEMVKELDFDVLVPWVTIKDEPLVYFVENEYEKREQIQNI
                     IDRVREGENS"
     gene            complement(26675..28297)
                     /locus_tag="NBK22_000358"
     CDS             complement(26675..28297)
                     /locus_tag="NBK22_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD2413575.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            28429..28590
                     /locus_tag="NBK22_000359"
     CDS             28429..28590
                     /locus_tag="NBK22_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413576.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKTIVLSYDLTNLTNFTRTAFKNIY
                     TLLTNIYLL"
     gene            complement(28606..28716)
                     /locus_tag="NBK22_000360"
     CDS             complement(28606..28716)
                     /locus_tag="NBK22_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413577.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVEREKWYFSGKEV"
     gene            complement(28816..29352)
                     /gene="nrdG"
                     /locus_tag="NBK22_000361"
     CDS             complement(28816..29352)
                     /gene="nrdG"
                     /locus_tag="NBK22_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCD2413578.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCTERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            complement(29349..31199)
                     /gene="nrdD"
                     /locus_tag="NBK22_000362"
     CDS             complement(29349..31199)
                     /gene="nrdD"
                     /locus_tag="NBK22_000362"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCD2413579.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAAGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            complement(31445..32815)
                     /locus_tag="NBK22_000363"
     CDS             complement(31445..32815)
                     /locus_tag="NBK22_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCD2413580.1"
                     /translation="MNSDNMLLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKPWIKWVNTALTLAVILAMLINIAP
                     PEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            complement(33137..33232)
                     /locus_tag="NBK22_000364"
     CDS             complement(33137..33232)
                     /locus_tag="NBK22_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413581.1"
                     /translation="MGGFVNSLIMKDLSDNDMRFKYEYYNREKDT"
     gene            complement(33308..33913)
                     /locus_tag="NBK22_000365"
     CDS             complement(33308..33913)
                     /locus_tag="NBK22_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCD2413582.1"
                     /translation="MNMPLMIDLTNKNVVIVGGGVIASRRAQTLSQYVEHMTVISPTI
                     TEKLQKMVDKGVVIWKEKEFEPSDIVNAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKVLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVR"
     gene            complement(33976..35856)
                     /locus_tag="NBK22_000366"
     CDS             complement(33976..35856)
                     /locus_tag="NBK22_000366"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCD2413583.1"
                     /translation="MKLNTSNSPFTEKQVSQINNLLQTLTESQQQWLSGFLLANSNDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAEHICKRVDCDIDYEED
                     AEKWMADIINMIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRD
                     VY"
     gene            36060..36143
                     /locus_tag="NBK22_000367"
     CDS             36060..36143
                     /locus_tag="NBK22_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413584.1"
                     /translation="MNAYSNASLLFFIYLLFIMYQISLSVK"
     gene            complement(36251..36748)
                     /locus_tag="NBK22_000368"
     CDS             complement(36251..36748)
                     /locus_tag="NBK22_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD2413585.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISTNIEILL
                     QESSS"
     gene            complement(36758..37018)
                     /locus_tag="NBK22_000369"
     CDS             complement(36758..37018)
                     /locus_tag="NBK22_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413586.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVLEKCITLYNVCSRCKRALILSEHSKKNIFNIKKAV"
     gene            complement(37400..37513)
                     /locus_tag="NBK22_000370"
     CDS             complement(37400..37513)
                     /locus_tag="NBK22_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413587.1"
                     /translation="MNGILIKCLNIGGQYYLQTRMLSNVDNAEKTISRNSI"
BASE COUNT        11939 a         7168 c         5702 g        13301 t
ORIGIN      
        1 caccattttt caaaataatg gcttaaatat cacatatcat taaatatagc gttttataac
       61 ggaggcgttt gttatgacta aagaaacaga taataaaaaa acagtagcga cttctgacaa
      121 caaagacaca cagcaacaaa atcaacctga agaagatgaa cgtcaaggtt tgaatggtta
      181 tcgtaaaaca gatcttgatt tagaaatcga gcaagagcta cgtgaaatga tgaaaacagg
      241 agaaaatgaa acgaaaaatg actataaaaa gtttaaagtg ttttcactta tttcaacact
      301 tgtcattgtc attttagcaa ttataagatt tgttcataaa atgatgtaat tagagtgaga
      361 cattgtttta tgtctcagga tccagttatt cattatatct acaatattta cgattatata
      421 aataacccga gattttagta tgattcattt cactaaaatc tcgggtttct atttgataat
      481 ttttaatggg atatggcatg tatacgttct tgccttttat ctcatttcca atgattaatc
      541 tggatattgt tctaaaaatg ctttcgcttc tttattaact gttttaaaat caataccttg
      601 ttgcatcgct gcaaagacac atccacaata acactgccta aagatattat agtcattaca
      661 catttctatg gaacgctcat aacctttact tttcttaaaa tcacttggca aatagttcac
      721 atcgtatatt ttttggacat ccataccaag ttcattgatt aattgtgcgt tctttttagg
      781 tgataatgtg attgcactgc caaaataatc atagccatgt tctacagctg ctttcgctac
      841 aatatccaaa cgcatttcaa aacaagcggt acaacgtaag ccgccttctt tttcatcagc
      901 taattcttta tccttcacca ttttcacaaa cttatgcggt tcataaggtg cttcaatata
      961 cttcacattc gcaccagtct tgcgattaaa atcttccaca aattgttctt gcactttagc
     1021 acgtcgtaaa tattcatttt tcggatgaat atttgaattc gcgaaataaa ttgcaatgtc
     1081 tgcatattgt gttaaaaact ctaatgtata tgtactacaa ggtgcacaac aactatgcaa
     1141 taagatttta ggtctgattg cttctctttc ccactgcccg attaatttct tcaacacctt
     1201 gtcataatta attttttgat ttttcatttt actgataata ggttcagcat taatcatgat
     1261 ttaacctccc acatttaatc attaacttct attatatatg attcatatta aatgtcagtc
     1321 aaaaaagtta gaaattcatt ttaatgcatt tatatttcga aaatccctta tgtatctaaa
     1381 agcatttttt aaacttgaat tttaaaacac taaacaacac atacgtctct gtgtcatttt
     1441 cattttttgt atgtcatata tatgtttact tcatttaaat caatttcatc ttacaattta
     1501 tcgtgtattt tacaaaagat tgacttcaat tcatcgtaaa agttatactt ttgccatttt
     1561 ttaatgtaac atggtgttag taataaaaat aatacattga ggtgttttac atgacagcat
     1621 tattccctta tatcgctttt gaaaattcaa aagaagccct tgcatattac gaagaagtat
     1681 ttggtgcaac tgacgttaaa cgtttagaag ttggcgaaga acaagcgtca cattttggta
     1741 tgactaagga agaagcgcaa gaagcaacta tgcatgctga atttgaagtg cttggcgtaa
     1801 aagtgttatg ttctgattct tttggtcgcg ctgacaaaat taataatggc atatcattat
     1861 taattgatta tgatgttaac aataaggaag atgctgataa agttgaagca ttctatgagc
     1921 aaattaaaga tcattcttca attgaaatag aattaccgtt tgctgaccaa ttctggggtg
     1981 gcaaaatggg cgtctttacc gataaatacg gtgttcgttg gatgttacat ggtcaagact
     2041 atactgcaat ccaacaataa tgttattgat aaagatatca tttaataagc ttatgttatt
     2101 tagcaaggca agccactctc gattcatggc ttgccttatt ttattgtata tttttaatta
     2161 tcgttgctgg cccttgattg acaatcgtat taaaatgcat tattaagtca actttatcta
     2221 tacggtttgg atcttctacc caatgtctta tcaaagacaa tcccgcacct gaaacataac
     2281 tcatgaaata ggaaaatggt ataccattaa tttgatcatt ttcattttta taacgttgta
     2341 aatgacacat aattaaatca taaagttttt cgtggatatt tgaatcttta cccatacgaa
     2401 acatcaattg ataaaatgcg atgtcttttt ctaccatttc tattaaaacg gtcataattt
     2461 gatgtatgtt atccgtggat aacttaactg ctccatttaa cttctcatca tgaatgaagt
     2521 ctcttatttc ctccaactgc tggtcctcta atttttcaag caaatcatat ttatcataat
     2581 aatgcgtata aaatgtacta cggttcacat cagctaaatc tgcaatttgt tgcacagtaa
     2641 tctcttctaa ttggtgttga tgtaaaagtt caataaatgc atttctcatt gcaacttgtg
     2701 attttctaat acgtcgatct atgatcattt gtatcaagac ctccccaatg attattaaca
     2761 attataaacg ttatgttcat tatcctacaa atctccaaca ttgatgattg gcacacaatg
     2821 tttacctgtt taatataggt gatacagaca aacagaaaaa ggtgataaca atgaaccaac
     2881 atctactagg aaatccaaaa ttaactgtaa ctcatgtcaa tgaagtgaaa gccggtatta
     2941 accacatcgt tgtcgacagt gttcaatatg gaaatcaaga aatgattatg gaaaaagatg
     3001 tcactgtgga aatgcgcgat ggcgaaaaat tatatattaa tattttcaga ccaaataaag
     3061 atggcaaatt ccctgtagtt atgtctgcag atacttacgg taaagataat aagcctaaaa
     3121 tcaccaatat gggtgccctt tggccaacat taggcgccat tccgacatct agttttacac
     3181 ctgaagaatc accagaccca ggattttggg tgccaaatga ttatgttgta gttaaagttg
     3241 cattacgcgg tagtgacaaa tccaaaggcg tcttatctcc atggtcaaaa agagaagcgg
     3301 aagattatta cgaagtgatt gaatgggcag caaaacagcc atggagtaat ggaaatatcg
     3361 gaacaaatag tgtttcttat cttgcggtga ctcaatggtg ggtcgcatca ttaaatccac
     3421 cacatttaaa agcaatgatt ccttgggaag gcttaaatga tatgtataga gaagtagcct
     3481 ttcacggggg tattcctgat actggctttt atcgtttctg gatacaaggt atttttgcga
     3541 gatggacaga taatccaaat atcgaagaca tgattcaagc acaacaagaa caccctttgt
     3601 tcgatgattt ttggaaacag cgtcaagtgc cattatcaca aattaaaaca cctctactaa
     3661 catgtgctag ttggtctaca caaggtttgc acaaccgtgg ctcttttgaa ggatttaaac
     3721 aagctgcatc tgaagaaaaa tggctatatg tgcatggacg taaagagtgg gaaagttact
     3781 acgctagaga aaatctcgaa cgccaaaaat cattctttga tttttacctt aaagaagaaa
     3841 ataacgattg gaaagatacg cctcatgtca tatatgaagt tagagatcaa ttttataaag
     3901 gcgaattcaa atcagcgtca gcgttccctt tacctaacac agaatataca ccattgtttt
     3961 tgaatgctga aaatcacaca ttgaatcatg caaagattag tagcgcgcat gtcgcacaat
     4021 atgactctga agataaacga caagatgtaa gttttaaata tacgtttgac aaagatactg
     4081 agttagttgg aaacatgaac ttaaaactat gggtaagcac taaagattca gacgatatgg
     4141 atttatttgc aggtattaaa aagttagatc gtcgtggtaa tgaagttaac ttccctgatt
     4201 ttaatcatat tgaaaatggt caagtagcta ccggttggtt acgcgtatca catcgtgaat
     4261 tagatcaaga aaaatccaca attgcgcaac cttggcataa acatgaaaca gaattaaagt
     4321 tgtcacaaga tgagattgta cctgttgaaa tcgaattgtt accttcaggc acgctattta
     4381 aacaaggcga aacattggaa gttgttgtaa aaggtagtga aattgtaatt ggtaatagta
     4441 cccctggcat gaaaacgcgt tatgaacatg aagaaaccgt aaataaaggc atgcacatga
     4501 tttatactgg tggtaaatat gattcacaat taatcattcc tatcgttaat tgatatgcag
     4561 caattacggt cgcttttgat taaaagtgac atagtgatag aactgtataa caagagcagg
     4621 ccacacgctt ggaatcttaa accaaggtgt ggcccttttt attattgatg gctatttgat
     4681 tttataaatc ttgtcactaa acttactaca agcaatatta aaccactaaa taaaaccatt
     4741 attgctgtat tactttcgtt gtttactcct gtttttggca aatctttcaa ttgttgctta
     4801 tgtcctgact tgctcttgct atccaaatga atactttgtt gtttagccat ttgtttatta
     4861 ctttttgtgt gagtatcatt tgctttaaca cttggattta cagcaccatt attcgatgtt
     4921 acatttgcgt ttttcaactg cttgataact tttggatctt tcgtgccatc cattaaatta
     4981 tctggatgtt ttagtaaatg tctacaatcc gatactattc cagatttttt tgcctttacc
     5041 gcattattct gcacctcatt atcatctttc aactgcttga taacatctgg atctttcgtg
     5101 ccatctatta aattatcaag ttttttcaca cgtgctatgt cactaactgg cccaggataa
     5161 tttgcatccg gttgtctaac tggtattaaa ttgctgtttt cattcacacc ttcaggcttg
     5221 ttattttcgg cgttcgcatt atccgtaaaa atcaacactg ataaaattgc aacaaataaa
     5281 cttataactt ttttcatcat atccaccttc ttcagaccat cattctattt tgtataattt
     5341 tattatataa ttgctatcaa ttcaatattt ttacttcacg aaactaaaca acatgtcatt
     5401 aacttatctc gttaagttaa gaattctatt ttctaccaaa taaattctgc atactgtttt
     5461 agataatcaa ttcacaaaac tatttcttta taaatatgac catcatttaa cactgataaa
     5521 attgcaacga taataccttt acttctatta cgctaaccca attggcataa aaataaaaac
     5581 ccctaatgga taactacatt gttatctact agaggtttct aacttatact attttataac
     5641 actatcgtat tttctttttc atgaatcatt tcaataatga cattatcttc attcattact
     5701 gctactttag gtgcatggtt tttaatttct tcttcattca actgtgcata agtcatgatt
     5761 atgactacat cgcctacttc aacaagtctt gacgctgcac cgtttaaaca aattttacca
     5821 ctacctcttt caccagctat tacgtatgtt tcaaaacgtg caccattatt attattcacg
     5881 atggctactt tttcatttgg caagatgtct accgcttcca atatatctga atcaatcgta
     5941 atgctaccta cataatttaa atttgactca gtcactcttg ctctatgaat tttagcattc
     6001 atcattgttc ttatcacttt attcagctcc aattattata ttatctatta aacgcgcttt
     6061 tgaaaatttg acagctaacg agataaatat gcgtccagtt atttcgtgtt gttctactaa
     6121 ttgaggataa ctataaacag caacttcttc aatgcgtcca cttatatgtg attcaagata
     6181 ttcagtaacc ctgtctataa ttactttact ttgacgttca ccgtcttgat acaacgcttg
     6241 tgctaatagc aaacttttac ttaaatgtac cgcttcttgt cgttctttct ccgttaaata
     6301 aacatttctt gaacttttcg ccaaaccatc tgcttctcga acgatatcaa taccaataat
     6361 ttcaacggca tgattgaagt cttttaccat ttgctcgaca atagccaatt gctgggcatc
     6421 ttttttacca aaataagcat aatccggcat aacaatatta aatagcttat taactactgt
     6481 taccacccca tcaaaatgcc ctggacgctt cgctccttct aacacatcag ctaatggacc
     6541 tactttgaca tcaataccta attcacctgg atacatatct tctactgcag gatgaaaaac
     6601 aatgtccgcc cctacttctg atactaattc taaatcttta tcaatttgtc tcggataagc
     6661 atcgaaatct tcgtttggac caaattgtaa tggattaaca aatacactca caactgtaat
     6721 atcatttgta ctaactgatt cgcgtaccat cgttaaatgt ccatcatgta aggcacccat
     6781 tgttgggata aaaccaatcg ttgtgcctga gcgtttggct gctttaacaa tgtgttgcat
     6841 ctcttttacc gtagtaatca gcttagtcat tgttattaac ctcattcata attttctttt
     6901 tatacgtata ttcttctgat ggaaatgcac cagatttaac ttcttgatcg tattgcttta
     6961 aaccatccac accaacacta aaatcagcaa attgcttcac aaatttcgct ttatgttcaa
     7021 caccataatt taacatatcg tgataaacca atacttgacc atctgtacct tttcctgcac
     7081 caataccaat gactggaatt gttaagtgct tgctaatttc ttctgctaaa tcatttggaa
     7141 ttgcttcaag tactaacgca actgcaccag cttgttctac atttttcgca tctaaaataa
     7201 gttgctccgc tgcttctttc gttgcacctt gtaatttata ccccataacg ccaacacttt
     7261 gaggtgttaa tcctaaatgt gcaacaacag gaataccaat tgccgttgct ttttcaataa
     7321 atggtgtaat atgcgctcct tctgctttaa ttgcatttgc attcgtctcc tgataaagct
     7381 ttagagcatg atttaagtct tgtgtcatag agatgcctac tgcaccaatc ggcatatcaa
     7441 caactacaaa tgtatttggt gcgcctcttc ttactgcacg accgtgatga atcatatctg
     7501 ctaacgtcac ttgtacggta ctttcataac ctaatacagt cataccaagt gaatccccaa
     7561 caagaatcat atcaataccc gctgcttcca cttgtttagc acttggaaaa tcataagctg
     7621 ttaccataga aattttagtt tgcttttgtt tcatatctat taattgactt actgttttca
     7681 atgttattca acctcttttt gcagtattat tagaaatagt ataatgaatc ttttatagaa
     7741 aggaaagaag aagatgttat cagttgcgat tatcggccca ggcgctgtcg gtacaactat
     7801 tgcctatgaa ttacaacaat cattgcctca tacaacgctt atcgggagac acgccaaaac
     7861 aataacatat tatactgtac cacatgcacc tgcacaagat attgttgtga aaggttatga
     7921 agatgtcaca aacacatttg atgttattat tattgcagtc aaaacacatc aacttgatgc
     7981 agttattcct catttaactc atcttgcaca tgaggacacg ctcatcattt tagcccaaaa
     8041 tggttatggt caactcgaac atattccatt taaaaacgta tgccaagcag ttgtctatat
     8101 aagtggtcaa aagaaaggcg atgttgttac acacttcaga gattatcaat tacgcataaa
     8161 agataatgca ttaacacgac aatttagaga tttggtccaa gacagtcaaa tcgacattgt
     8221 attagaagca aatattcagc aagctatttg gtataaattg ttagttaact tgggcattaa
     8281 ttctatcaca gcactcggta gacagacagt tgcaatcatg cataatcctg aaatacgtac
     8341 actgtgtaga cagctattac tagatggttg tcgtgttgca caggcagagg ggttagactt
     8401 ttcagaacaa actgtcgata ctattatgac tatatatcaa ggttatcccg acgaaatggg
     8461 aacgagcatg tattacgaca ttgtgcatca gcaacctctt gaagtcgaag cgattcaagg
     8521 ctttatttat cgacgtgcac gtgaacacaa tcttgacacc ccatatcttg atactattta
     8581 tagcttttta cgcgcgtatc aacaaaatat gtaagcacat atataatata aaaagacctt
     8641 aacgatgcat ttagaaccac ctgttcctaa tatcgctaag gtcttttaat tatattagac
     8701 tattcagctt ctctaatttc ttctgccaca tctttatagt ctattttcgc tttaacaaac
     8761 gtctcgttat ttaccgggaa atgttgttgg aatgtttcaa agttttgtat ctcaacgaca
     8821 acgtcatcca cttcaaagtc aagaacatgt ccaccataag ctctttcatc atctgcaaaa
     8881 tgtatatgaa aaccagcaga ccctacgcca tgaaataatt ctggtgtaaa aaatccaaca
     8941 atggtaccac gaatatcttg tcttttttcc tcaggttgtc tgcgtgctga atcaatcaaa
     9001 cgtgtatatg gcggttgctg agcaggcatc attcgtacat gcatatgttt aaatgtgcca
     9061 taaattttaa cagccgaaaa taaattctca cttaacattt catttttaat ttgtgcgaat
     9121 acatcatctt gtgataattg ttgcaatgaa aatgtcttac tcgctttaaa attagtaatc
     9181 gatgcatacg gtactttctc atcgcctttt aattctataa attctttatg ctcgttagca
     9241 tgatatgcct tcccgtctaa aaatattact tcgccatcag accctgttaa cgttgcaatc
     9301 cctaaatttc catgttctaa caattcatta attgtagctg ttccttctag taagccagcc
     9361 attaacgtac ctaatgtacc atgttgatac aagacattcg tcattccaaa accatctccc
     9421 tatagttatt cctcgttgtc atgctctact tcgtgattaa tgcagaacta ctttccttta
     9481 tactcaatga cgcctcgaca tttctattca tgccactaaa ttttcacgag tcataatatt
     9541 ttattcaatt attattttac gcaaaagtat tgcctctatc aatgcttgct aaaaaaaatg
     9601 aacctatcag tgccacgtgt tactgcaaca ctaatagatc ctcttaccat taatatattt
     9661 ctaattaata cttaaaatag gcttagtctt ctaataaata tttaattgaa tcaaatgtat
     9721 cttctaatgt tttaacagat ttatcgaaca acgcttgttc ttcggcactt aatggcattt
     9781 cataaatttt aactgcgcca tgttggttaa ctaatgttgg tacacctagg taaacgcctt
     9841 tgtgaccacc atattgacca tctaattgta tagaaacatt taagacatta ttttcattat
     9901 ttaaaatggc ttttgaaatg cgcatcaatg ctaatgcaat accatagtat gttgaccctt
     9961 tagcttggat aatttcataa gcagcgtcac gtgtatttac ataaatttct tccgctttag
    10021 cttcgctacc agtttgttct tttaatgtgt catatactga aataccagct acatttgctt
    10081 gtgaccaaac tgcaagttca gtatcaccat gctcaccaat aatactagcg tcaacacttg
    10141 aaggtgcaac accaagttct tggctaatta aatattgtaa acgtgcactg tccaatacag
    10201 tacctgaacc gataacacgc tctgctggta aaccagtata ttcttttaca aatcttgtta
    10261 aaatgtcaac agggtttgcc gcgattaaga aatatccatc aaagccacta tccataacac
    10321 tcttaacgat gctcttcata atcttagtat ttttttcaac taattgtaaa cgtgtttcac
    10381 ctggcttttg aggtgcacca gctgtaataa caactaaatc tgcatcttta cagtcttcgt
    10441 attcacctgc tttcacatca actggtgaag gactgtggac tgtaccatgg tttaaatctt
    10501 gaacatctgc ttttactttg tcttttgcaa tgtcaataat tacaaattca tcagcaacac
    10561 cttgcgtaac cattgcaaag gcatagcttg atcctacaga tccatctccg attaatacaa
    10621 cctttttacc aaatgttttc ataattatgt ctcctaacct aattgaattt attttacacg
    10681 tgactacacg taaaaatatc tatactgaaa ttaaagatta acaattttat tttattataa
    10741 tattatgcta ccaaattaca aacataaaac tgagatgtaa gcggagtcaa attacactct
    10801 aacgccaata aatattgata tctcaagctt tagcacgtaa tttgaaattt gtgacaattt
    10861 ttagcattct aaaattgcgt aacgacgcta tttaaaggct ttaacaaact attgagcttt
    10921 ctattttttt cattttaaaa aggatggcta ttcgacttaa atgacttact atataacctc
    10981 ctattttccg ccttaccctc atcatatttg atgcatcatt ttatagtcaa agaattacat
    11041 ttcatacaaa ctagccaatc agacataaac atgaaattga ttttaaaaat ataatgtgca
    11101 aaattgttag aaaattagca aaaaatagta cttattcatt taatatctgt tataatcagt
    11161 tttatcattt aaaaaatcta tggatgttat caagacaagt cattaatacg taacataaag
    11221 ttgtgatttt tcagaatttt tagacaaaat attgattatt tgtgctatga tataacatat
    11281 taaaaaacac cgtatagaat tgtttatgaa tgtttgggag gaaagtttat gggaagtttt
    11341 ttcaataaaa tagcacgaaa agaggatccg gctatctatc aaaataaaga tggtcattta
    11401 aagcgtacac ttcgggtgcg tgatttctta gctttaggtg taggaacaat tgtatcgaca
    11461 tctatcttta cgctacctgg cattgttgct gcagaacatg caggaccggc cgttgcgtta
    11521 tcattcttac tcgctgctat tgttgctggt ttagttgcat ttacttatgc agaaatggct
    11581 gccgctatgc catttgcagg ttcagcctat tcttgggtca atgtattatt tggtgaattt
    11641 tttggatggg ttgccggttg ggctctatta gctgaatatt ttatcgccgt agcctttgtt
    11701 gcatcaggat tctcagcgaa tttacgcgga cttgtgaaac caattggcat cgaattacct
    11761 gcagcattat caaatccatt tggtacgaat ggcggtttta tcgatattat tgctgctatc
    11821 gttattttat taactgcatt attactatca cgtggtatgt cagaagcagc acgtatggaa
    11881 aacattttag ttattttaaa agtattagct attattttat ttgtaatcgt aggtttaaca
    11941 gcaataaatg ttagtaacta tgtgccattt attccagaac acaaagtaac tgctacaggt
    12001 gactttggtg gatggcaagg catatatgct ggtgtttcaa tgattttctt agcgtatatc
    12061 ggtttcgatt ctatcgcagc aaactcagca gaagcacttg accctcaaaa gacaatgcct
    12121 agaggtattc ttggttcttt aagtgttgct atcgtattat ttattgctgt agcacttgtg
    12181 ttagttggta tgttccatta ctcacaatac gcaaacaatg ctgaacctgt tggttgggct
    12241 ttacgtcaaa gtggtcatgg tgttgtagca gctattgttc aagctatctc tgttatcggt
    12301 atgtttacag cattaattgg tatgatgtta gcaggctcac gtttacttta ttcatttgga
    12361 cgtgacggct tattaccttc atggttaagc catttaaacg acaaacattt acctaatcgc
    12421 gcactagtta tacttactat tattggtgtt ttaattggtt ctatgttccc attcgcattt
    12481 ttagcacaat taatttcagc aggtacactt gttgcattta tgttcgtttc attagcaatg
    12541 tatcgtttga gaaagcgtga aggtaaagat ctaccaattc ctgcatttaa attaccttta
    12601 tatcctgtgt taccagcagt tacatttgtt ttagtattgc tagtattctg gggattagga
    12661 ttcgaagcga aattatacac tttaatttgg tttattgttg gtatcattct atatttaagt
    12721 tatggactta gacattctaa gaaaaatgac gtagctgaat accacccacc aaaataaaaa
    12781 tcaaaaaaat catttcccat ctgattaaca tccagatgtg gaaatgattt tttattttat
    12841 attaagataa tcgccattac caaccttgtc cagatatgtc atattgatct aagttacctg
    12901 cttccaaagc agtcagtgca tcttctatag tgtttaacgc cgtgtctaat tgctcatatg
    12961 ttatcaccaa tggcggttgg aatcgcaaca catttcctgc tacagctata ataactacgc
    13021 catgctcaaa gcagtaatta caaattttaa gtgccgcact ggcatcacgt gttttgaatt
    13081 ttttgtcgga aacaatatca ataccaatgc tcagaccttt acctctaaca tcgcctacac
    13141 tattgtattt agatacccat tgatccattc gtttcctaac atattcccct ttttccgcac
    13201 tagcctgaag aagcgactga tcttcaatca tttgaattgt ggctaatgca gcttcacaac
    13261 taactggatt agcacctgtt gtaaataaat gtgctggcgc ttctaaacaa ttcataatct
    13321 ctttgcgtcc aacaattgct gacataggca taccacctgc taaggatttt ccaaaagtga
    13381 ttaaatcagg cgtaaaatta aaatgcgaga ctgaactcca tgtacctgtt cgcccaaaac
    13441 cttgttgaat atcatcgaca gcgattaaaa taccatgttc acgacaaatc ttttctaacg
    13501 cttcaaaata ccctggaact ggttctaaaa gtccaccatc gccttgtatc gtttcaataa
    13561 caatacatgc tacttcgtca gcaggtacat atttcgcaaa catttctttt aagggtgcta
    13621 aatattcttc tactgaatta gcttgcggct gctcgtacat accacgatat ttatctggaa
    13681 acggaatatg ataaaaacca ttcaataatg gaccataatg tttgcgcata tttaaactaa
    13741 tagctgacat agacaacgag ccaaaagttg aaccatgata tgcattagtg aaactaatga
    13801 tataagggcg ccctgtatat gctctggcaa atttaatgat gccatcattt gcgtctgatc
    13861 cggttaatcc gaaagtcact cttttttcaa aatctccagg tgcaatctca caaagcttct
    13921 tagctaaacg taccaacggt tcatgataca tatatgctgg tgtataatga ataaatttat
    13981 caacttgtgc ctttatcgct tctgttactt ctctaggtgc atgacctacg ttttgagaac
    14041 tcgcactcga taacaaatcg atatatgtct tcccctcaat atcaaccaat gttgctccat
    14101 atccatgatc aatcgctaac ggataatatt taatacgtcc tgatttcgca aaataatgtt
    14161 catcctcttg aattaattga tgtgctttac tcatatacat tcccctccca ttgacagtat
    14221 taatatgcaa gcaaagtggc ctctcttaaa tcgattataa aatccgattt aaaatcctgc
    14281 tcaaataccg tcatgcctct atgaatacta tccaatatac taataaaatt cagatttgta
    14341 aatatattct gataattaag aattttatat caatctactt tgtttatgcg cataattttg
    14401 ttcgattagg gaattgttta ctatatttca tttgactctg aggtgatacg aatgcgttta
    14461 aaagtattgt tccatttcat tgctgctatt ttcatatcat tcatgttgct atggatgacc
    14521 atgttgtttg acttaataag caatcaatca catttaaaag ctttattatt gaatttagat
    14581 tttctaattc cttctgacaa tacaccatat attctagaaa taatatgtca cttactaatt
    14641 ggtagcgtca tttattttgt atttgtgttg ctgtttcata cttctaaaag gctttactat
    14701 ctatgttata tccctttatt tttcttattt atagcattgt atcccttttt agtatttatt
    14761 gcgcaacgtc caatttttca atttagtgtg acagaattaa tcggttggat tattacacat
    14821 attttcttta tgtcattaat agcgttagtc atacctagaa taaaataata tttttaatta
    14881 aactgacaat tatttttcac attttataca cttttcaagg tttatgactt gagaaatgtt
    14941 ctcattttta tcatactgga ggtgtattga aaagttaagg agtggaaagt atgaataaag
    15001 agcaattaga aaaaatgaaa aatggaaaag gctttattgc cgcattagac caaagtggtg
    15061 gtagtacacc aaaagcactt aaagaatatg gtgtaaacga agatcaatat agcaatgaag
    15121 acgaaatgtt ccaacttgtt cacgatatgc gtacacgtgt ggtaacttca ccttcattct
    15181 caccagataa aattttgggt gctattcttt tcgaacaaac aatggatcgc gaagtagaag
    15241 gcaaatacac tgcagattac ttagctgata aaggtgttgt tccgttctta aaagtagaca
    15301 aaggtcttgc tgaagagcaa aatggtgttc aattaatgaa accaatcgac aacttagaca
    15361 gtttattaga ccgtgcaaac gaacgtcaca tttttggtac aaaaatgcgt tctaacattt
    15421 tagaattaaa tgagcaaggt atcaaagacg ttgttgaaca acaatttgaa gttgctaaac
    15481 aaattattgc taaaggttta gttccaatta tcgaaccaga agttaatatt aatgcaaaag
    15541 ataaagctga aattgaaaaa gtattaaaag ctgaacttaa aaaaggttta gatagcttaa
    15601 atgctgatca attagtaatg ttgaaattaa caattcctac tgaaccaaac ttatacaaag
    15661 agttagctga acatcctaat gttgttcgtg tcgttgtatt atcaggtggt tacagcagag
    15721 aaaaagcaaa tgaattactt aaagataacg atgaacttat cgcaagtttc tcacgtgcat
    15781 tagctagtga tttaagagct gatcaatcta aagaagaatt cgataaagct ttaggtgatg
    15841 ctgtagaatc aatttacgat gcttctgtaa acaaaaacta atttatgcat taaacgtatt
    15901 ttttgatata aactactaaa aaggactcat acatgtgctt taatggcatt gtgtatgagt
    15961 cttttttgat taaagttgat tttgccgtat gtattagatt acgtaaaaaa atcggacagc
    16021 tgaaaaatct ttaagatttc tcagttgtcc gattttaaat atagctatta gaatttagtt
    16081 ttcgtagtaa cctaattcta agtcttttga agtttgttta cggatttttc tcattaattt
    16141 ttcgtcttca attaatgatt caccgtatga tggaatcatt ttcttgattt taggtgccca
    16201 ttcagttttg tattcaggga agttacgttc taatacttct aacgcaactg aaactgaagt
    16261 tgaagcccct ggtgattcac ctaataatgc gattacagtg tggtcttgtg agttaaccac
    16321 ttctgtaccg aattggatga atcctttacc gtgttcaggt gtatctttga taacttgtac
    16381 acgtttacca gcagtgtata attgccaatc ttcattacgt gcttctggat agaaagtacg
    16441 taagtggttc atacaacctt cttttgtcat aatcacttgg tcaaatgagt atttaattaa
    16501 aggtaagttt ttaactgctg ctgctaataa agttgtaatg ttgtatgttt taacagactt
    16561 gaataaatct aagttagaac catttttcaa gaatttaggt ccaacattag caaatggtcc
    16621 aaataataat gttctttgac catcaatgta acgcgtatct aaatgaggta cagtcattgg
    16681 tggtgtacca ggtggctctt taccataaac tttggcatcg tgttgttcaa taacttgtgg
    16741 gtttgtacaa gctaagaatt gaccactgat agggaatcca cctaaatgtt tactttcagg
    16801 gatacctgtt ttttgtaata atggaattgc tccaccgcca gcaccgatga atacatagtc
    16861 agttacttgt ttgaatttct caccagttag gcgattttta acagtaactt cccattgccc
    16921 atttgataat tgttcaaaat caacaacttc atggttaaat tgcactgtag catttggatg
    16981 tgcttcaatg cttttagcca ttttacgtgt taattcaccg aagtttacat ctgtaccttc
    17041 gtcaatttta cttgccgcca tgataccagg gttatcttca cggcctttca tcatcaatgg
    17101 aatccatttt ttcattactt cgatgtcctc agtatattcg atattatcga acatagggaa
    17161 agctttcatc gcttcgtaac gatcttttaa gaatttaaca ttgtttttac ctctaacata
    17221 actgatgtgt ggtaatggat tgataaattc tcttgggttc tcgatgctac cgcttttcac
    17281 taagtgaccc cagaattgtt ttgaaatctc aaactcttcg ttaatcactt tcgctttttc
    17341 gatgtcgata gaaccatcag gttgtaaaac tgtgtagttc aactcacata atgctgcatg
    17401 acccgtacca gcattatttc tttcgtttga actttcgatt gcaggacgat ccaagcgttc
    17461 gtaaacgtgg atattccagt ctggctcaat ttcttttaac attgaaccaa atgttgtgct
    17521 aagtacaccg gctccaatta aaacgatgtc tttactatta gacttagcca ttggtttcac
    17581 ctctccaaaa attgtaaatg tgtatcatca atatgaaagt tacataaaac tgacatattt
    17641 ctttaaaata tcaacgccat agataacttc ctgttttaat tgatacgctg taacaaaata
    17701 ctatagttag tgcttacatg tatatgttaa agcaagcagt ggtaaatgta aattataatt
    17761 attcattaac tttgcaatat attaaatctt ttattcatag aagataaata tcaaatcgat
    17821 cataattatt tgacaacaaa tagctaacga ttgttttaat ctacatttgg attatagcat
    17881 tttaaaccta tactctattt tgatacaata taagtgtaaa atcaatcata aaaaggagat
    17941 tcaatatctg catccaagaa aaacatcaca attaccttta atattgaaaa ctattttgaa
    18001 tctttcactt ataggcattg tgattatcgt ttttacacat gctgagttta agtttaatgc
    18061 cggtgatagt aatgtgtttt tattaacatg ggatattcga ggtttaacat cttatatttt
    18121 ctcattctta atactggttg cgcttgtttc taaactaatt ttaatattta taactagacg
    18181 acaaagaaca tcataaataa acaagcagtc cagtgcgtat catcatacct tacgcattgg
    18241 actgctttgc tatgtttatt gattgctatc tttttgttgt tgccattttt ctatttcaga
    18301 atctcgtagt tcaacacgac gaattttacc tgagtttgtt tttggtaagt cgtcaacgaa
    18361 ttcaatctct ctcggatatt tatatggtgc aacttcattt ttaacaaatt gttgtagttc
    18421 tttaactaac gtatcatcac ccgcagtatg gtcctgtaaa ataacgaatg ctttaacaat
    18481 atttcctcgt atatcatgag gttttgctac tacagcacat tctttaacgg ctggatgatt
    18541 cgttaacgca tcttcaactt caaaaggccc aatcgtatag cctgaactaa taataatgtc
    18601 atctcgacgt ccttcaaacc agaaataacc atcatcatct acatgagcta agtcaccagt
    18661 gatgtagtat ttacctgttt gcgctttcgc cgtacgttct ggctctttat aatacccttt
    18721 gaaaagtgct ggcaaatcaa gtggtactgc aatattccct ttcgtattag caggtacgct
    18781 attcccctca tcatctacta cagtgaccga actacccgga atgcctttac ccattgatcc
    18841 aatcctctgt ggtgtatctt ttaaaaagcc tataagcaag gtactttcag tctggccata
    18901 tccatctctt acagttaaat taaagtattt cttgaattgt tcaactactt ctcgatttag
    18961 tggctcacct gcagaaacgg cactatgtaa atgcgttaag tcataatcat ttaagttctg
    19021 taatttagcc atcatacgat attctgtcgg tgtacaacat aaaacattaa tttgatattt
    19081 ttgaagcaat tctaagtatg tttcaggact gaaccttcca ttaaatacaa aagcagttgc
    19141 acctgaacct aatacagata agaaaggact ccatacccat ttttgccaac ctggtgctgc
    19201 tgttgcccaa actaagtcat cttcattaat acataaccaa tgttttggtg ccatttgtaa
    19261 atgtgcaaat ccccatccat gacaatgtgt aacggcttta ggattgccag ttgtaccaga
    19321 tgtatatgac agaatcgcca tatcatcacg cgtcgtatct gccatttcta gtttgttact
    19381 tgcgttttct ttttcagctt caagtgaaat ccatccatct ttttgaccgg caataacaaa
    19441 tttagttaac gcatcatatt ctttaatttt ttcaaattca actgtgaatg gctctagtgc
    19501 aataactgca ttaatttcac catgtgtgat acggtattgt aaatctttag ttcttagcat
    19561 ttcagaacat ggaatgattg caacacctaa ttttaaagca gcaatatata attcatacgt
    19621 cgcaatagat cgtggcatca taatgagtac tttatcgcct ttagataaac cgtgcgatgc
    19681 taaaacatta cctactttat tagactgttc aatgagctgt tggtaagtga ctgatatatc
    19741 ttcgccttca gtattatgat ataaaattgc ctttttatct ggtatgtggc tatatttttc
    19801 gatttccgaa ataatgttat atttttcagg cgcgaataga gctgactttt gcataactaa
    19861 cttcctttca tacatccact tttcctgtga tgaacattgt aattttataa atgaattata
    19921 tatatcatac gcctatcttt acagaatttt caattaaata gggttaaata ccaaagtcct
    19981 cgacactaca ctttgacatg acgtagcatt caaggacttt caaatgattg agggttgata
    20041 tctcgggcta gaccatatca gctaattcaa tacgaatatt gtatgataat tcacgattaa
    20101 ttatttttac atctgcacct ttcgaagtgc cacgatgctt gtgtgtatgc ttgtactcag
    20161 ctgaattttg ccaatgataa aatgcttgcc tattttccca cagcgtaata atgatatagt
    20221 gtctaccagc tgttctaggt cttaaaaacc ttaatgcttt aaatccatca acgtttttta
    20281 aatgcttatt acgtcgcaaa aatttttgct cgaatgtttc ttcttgtcct tcgttaatat
    20341 ataaatgatt taacacacat aatgaatcat ttgataaatc atttatcgct tctagcacat
    20401 cgtaatatgc agtgtcatta tttttttgta ttgtgagaca atcatccagt tcttctatta
    20461 cataacttct atattcatca taaatgttca taataaatgc cttcatttca tttatatttt
    20521 tggtcatatt actttatatc tattactaat tcattcccgt atttattaat tacaatcata
    20581 gtttggcttc tttttaaaag ataagacttt gtaaaaagta ttaatatttc atgcaaatgg
    20641 ggacaggagt cgcccactat ttttgtgtct tcaatttcat gatcattatt taacattagt
    20701 catgaaaata gccgacaact tcatctgtga aatcaccggc cttttatttt agctaacttt
    20761 atttctgatt ttacgatttt aattgatcat acagagaaag tgatcttttt acaatttcta
    20821 aaaactcatg atctatattg gacatttgat gaaaataaga caaaatgttt tctgttagct
    20881 tctcttgttt tgggaatgaa tcatcttctt taatccaaat cgctaattcg cctaatggtg
    20941 ttttatcatc tttaaatgtt tgtatatatt cgtaaaagct catagtattc cttctctcaa
    21001 tttacttata taaatcctac cacgaaagct ttcaagaaaa cacaattaaa tgtctattta
    21061 gtgaactttt taaggttgtg cactctttta atgtctgcca attaggtcaa ttaatcatca
    21121 caatgtacaa ttaactctat tttcagttca tatactcaca caccgttttt gaacaacaca
    21181 ttaacttctc atttagataa aacgcaaaaa agcctggcac caatacaata gatgccagac
    21241 taagagtcta ctatataaat ttatttagcg tatggtttta cttcgattgc accttcattt
    21301 tcatcatgaa caccatgctt ataataatca atatattgtg gctctaaagg ctttctgcca
    21361 cgtataatgt ctgctgcttt ttcagctaac attaaaacag gtgcgtgtat attgccattt
    21421 gtcgtacgtg gcatagcgga tgcatcaact acacgtaaat tttccatacc gtggactttc
    21481 attgttaacg ggtcaactac tgccattgga tctgaagcag gacccatttt agcactacaa
    21541 gatgggtgta atgctgtttc accatctcta cgaacccaat caagaatttc ttcgtctgtt
    21601 tgcacttctg atcctggtga aatttctcca ccattgaatg gatccattgc tttttgagat
    21661 aagatatttc ttgctacacg aattgcttct acccattctt ttttatcttc ttctgttgat
    21721 aaataattaa agcggatact tggtttttcg aatggatctt tagatttgat tttcaagcta
    21781 ccacgagagt ttgaatacat tggtcctacg tgaacttgat aaccatgtgc gaccgctgcc
    21841 ttttgaccat catatcttac agctattggt aagaaatgga acattaagtt aggataatca
    21901 acttcgttat ttgaacgtac aaatccgcca ccttcaaaat ggttagatgc tgctgcacct
    21961 gtacgtgtga aaatccattg taaaccaata aatggcatgc gcttgatatc taagcttggc
    22021 tgtaatgata caggttcctt acatttatgt tgtatgtata cctctaagtg atcttcaaag
    22081 ttttcaccca caccaggtaa atgaacacgt ggctcaatgc cttttgattt taggaactct
    22141 gaatcaccga taccagataa ttgcagtaat tgtggcgtat tgaatgcccc accagacaaa
    22201 atgacttcat tagcatcgat ggtatgtaat ttgccatttt tcttatacgt aacaccagtt
    22261 gctcttctac cttcaaaatg aatttcagtt acaaaggcac gtgtttcaac ggttaagttt
    22321 ttacgcttca tagctggatg taaatatgct cttgaagctg acattcggcg accacgatgt
    22381 acttgactat cgaacggtcc aaaaccttct tgtctaaatc cattcacatc aggtgtttta
    22441 tgatagcctg cttcaacacc tgcatcaaag aatgactgga ataaaggatt cgttgctggc
    22501 cctcgtttta acttaattgg tccatcatgg cctctaaatt tatcataagg cgctgcaccg
    22561 tatgtttttt ctaatttttt aaaatacggt aaacagtgcg caaaatccca agtttccata
    22621 ccttctggtt ctgcccagcc ttcatagtcc attggattac cacgttggta aatcatgcca
    22681 ttaatcgaac ttgatccacc taaaacttta cctcgggcat gtgctacttt acgaccgccc
    22741 atatgtggtt cttcatctgt tgaataaatc caatcgtaaa atttattgcc tgaagggaac
    22801 attaacgcag caggcatttg gataaataaa tcccaaaaat aatcactgcg acccgcttct
    22861 aatactaaga cttctttatc tttatcttca ctcagacgat tacctagtac agaacctgca
    22921 ctgcctcctc caatgatgac ataatcatat gatttgtttt tgttactcat cgttatgacc
    22981 tccaaagttt gttgtatttc aaagtgagta ttgcatgaaa tatttgttag tggctgattg
    23041 gttatcgttg aattgctaaa cttagctaac caaaacactc gattttcctt ttacgattgt
    23101 tactaacagt catgaaataa tctatgattc attttgcgtt tagtccaaat tatcaatcaa
    23161 catttcgcca cttatacaaa acaaagtgag tgtcttgtgt tcttacaatg gcactcacct
    23221 tatctaattt ttatttgcta aaccaattca ctaattgtgg atttgtattt gttaaaatgt
    23281 gttttgaaac aaggtactct tctaagcctt ctttgcctaa ttctctaccg atacctgatt
    23341 gtttgtatcc accccatggc gcttgtgcaa aatatggatg gaaatcatta atccacaccg
    23401 ttccaagttt caacttgtta gcaacgcgtt gcgcttttcc aatatcttta gaaaatacag
    23461 cacctgctaa accatatata gaatcattcg ctaattgaat cgcttcttgt tcagtttcaa
    23521 agccttctac agtaacgaca ggtccgaaaa cctcttcttg tacaatacgc attgacgtat
    23581 cacaatttgt aatgactgtt ggctcgaaga atagaccatc ttttaaatcg tctctatctg
    23641 gacgtttacc accaacagca attgttgcgc cttctgcttt agctacatcc atataagatt
    23701 cgatcttatt acgatgttct gttgaaatca ctggtcccat ttcagtatca gcatcaaaac
    23761 cattacctaa tttgattttt ttcacgcgat caataagtgc ttgctcaaat ttgtctttaa
    23821 tactgttttg tactaatatt cttgatcctg ctgaacaaac ttgacctgca tggaaatatc
    23881 caccatttaa cgcttggtct actgccaatt caaaatcagc atcatcaaag ataatgtttg
    23941 gatttttacc gccaagttcc aaggcaatat tcgtaacatt attagcagca tttttcataa
    24001 tatgcttacc agtctcaatg ccacctgtaa atgatacaag gtcaacctct ttatgacctg
    24061 acattacgtc accaacttca gaacctgcac ctagaataag attaattgtt cctttaggga
    24121 aaccaacttc ttccattaat tcaaaaacac gtattgttgt taatggtgta atttcacttg
    24181 gtttcataac tagtgaacaa cccgtagcaa gcgctggcgc aattttccat gatgcttgta
    24241 ataacggata attccaaggt gtaatttgtg taactacacc tactggttct ttaacaattt
    24301 tgctttctgt atctggaatt ggtgaatcaa tcatttcgcc accgtcttta tctgctaatc
    24361 cagcaaaata cataaacaca ttatgaatat catccatatc tgcatatgat tcttctaacg
    24421 tttttccagt atctaatgtt tctaatcgtg ctaacgcttc gcgatgttct ttaatcttgt
    24481 ccgcgatggc acgtactttt ttacctcttg tttcagcagt ttcttgcgac cattcaccag
    24541 actcaaacgc acgtcttgca gctaagattg cacgttctgc atcctctttt gtcccttcag
    24601 aaaccgtaaa tatcacttct tgattgtaag gattgataat atctcttgta tttttattcg
    24661 cgctttcaac ccactcacca tcaatatatt gacgctgaga taaatgtttt aaaagttcca
    24721 ttcaacaacc tccgttaagt ttgttaaatt tttatttaac gaattttaca aacttatgtt
    24781 agcatagaaa tgaaaaatgt gtcaatttac aaaacaactc aagaattaag ttttgtaagt
    24841 ttgttagatt tttgtttaac gaaacattta actttaatgc tttaaatatt tttaatgtct
    24901 ttttttagaa aaggttttat aatgtattag tatttatttt aacgtgacca aaatgatgta
    24961 cggaggtgca atgatgactc gttctcagaa caaacagcaa caattagaac aagcaaaaga
    25021 tattgtaatt aattcaattg gacagacaat ggatttgtat ggcacgaaca gaagtgtagg
    25081 aaacttatat ggcactatgg tgtttgaagg cagtatgacg cttgatgaaa tgcgtcatca
    25141 attgcaaatg agcaaaccaa gtatgagtgc tggcgttaag aaattgcagg aatttgatat
    25201 tgttaaacaa caatttacga gaggaagccg caaacaacat tttatcgccg agaaagactt
    25261 tttcatcttt ttccgaaact ttttcactaa aaaatttcag cgtgaaattg acatcaatgt
    25321 cgaagcagta aaagatgctc aagctattat caatccatta cttgaaagca gtgatttaac
    25381 tgaagctgaa acacaagagg cacaaaaaat taaagctcag cttgatcata cacatgttta
    25441 ttacgaatgg ttagaacagt taaccgaagc gattgaaagc ggtgaaattt tcaaatattt
    25501 cccaattcca gaacagcctt ctgattctga aaattaaaat tgactcaaac aacgacaatg
    25561 atgattatgc atgataaaaa caatgcaata ggtccacgaa ataaacggta atttctatct
    25621 caataaatag aaattaccgt ttttgtttta tatgacaatc aattatggag gtattttcta
    25681 gggctttctt agattcgtta cattgcttcg ccaatatatt tcaattagct attctcgccc
    25741 tcacgtactc tatcaataat attttgtatt tgttcacgtt tttcatattc attttctaca
    25801 aaataaacta acggttcatc tttgatagta acccaaggta caagtacatc aaaatctaat
    25861 tctttaacca tctctaaact ttgaatagat ttttctctat cacttgaacc taatataact
    25921 gtacgccatc tcttcccttc aaaacatata aaatctccag taaataagta acggtgatga
    25981 ccatcatccc ataaaaatag tgtcgtccct ggtgtatgtc caggcgcggg aataacttct
    26041 aaatccttat ccaacattac acgattacta aattgttttt gaacagaaat cttatgtttc
    26101 agtgcagcca catcattttc atgtataaag taaggggctt caacttgatt agaacctcct
    26161 atagattcat gatcatgatt catcaatact tttgaaacac cacctaatga tgcaatgttt
    26221 tgttgggagt cacctaagtg tccggaatga tagataacaa tattacctgt ttcacgtttt
    26281 aaaataaaag atttaaatag aaatcgatta tcaaaaggca gttccgaagt aggtgtcgca
    26341 tataagtttt ttgtgatgga ttctaaactg tcatgtaatt tggactgttt atttttaaat
    26401 tgattagtca ttttatttca tccttcgatt aatttgaatt gtacatacgc aatacccttt
    26461 atttaattta ataatcaatg agagtctttc aaacttcgat ttagaatatg cttcatacaa
    26521 taaataagta cttggagcac taaaaaatta aatcttttag tacaacatta atgaaaacct
    26581 aaaagttcat cctacaatgc tactaaaaaa aggggaatgg aacagaaatg atattttcac
    26641 aaaattcatt tcgtcgtccc agacccgctt tgaattataa attatcgtct tgttcttctt
    26701 ctgatacttg aacgattcgc aatgaacgac gttcaacttc ttttaatttt tcagcacgcg
    26761 tttcaagttt aattctatcg cgccctaaaa tgattaaaaa tgatatcatc atgaaaataa
    26821 aaataacaat taatggcaca cttgccagta ttgaagcagt tttcaatact tctaatgcac
    26881 gttcgccacc aactagcatc aatgaaaatg gcaataagca caatgcaaat gcccagaata
    26941 aacgattggc acgtaatggt tcgcctacca cttttttctg agatgctgcc gctaaaatat
    27001 atgaacccga atcaaatgtt gttgctaaga ataagaaagc agatactaag aataatacaa
    27061 tcatcaatga tgggaatggt aaatgatgca ccacttcaat aatggttgcc tctgtaccat
    27121 gtgtatttaa atattgtgtt acattaaact gtccagaaat ttgtaaatac acagcatagt
    27181 taccaaaaat accaaagaat aatacgcatc caagcgttcc ataaataatt gttcctagca
    27241 cgacttcttt aaggcgtcga ccttttgaaa ttctagcgat aaataaaccg ataaatggcg
    27301 catatactaa ccaccatgac cagtagaata ttgtccagtc ttgtgggaaa ttcgtttctt
    27361 ttcgaccttt aataccaccg aatggttcta accatgttgc catatgaaag aaatctctca
    27421 acatatttcc gaaccctgtc actgtcgttt ccataataaa aacagtcggt ccaataataa
    27481 atataaaggc taaaagtaca aaggatagcc aaacgttgat atcacttaac ttttgaatac
    27541 cttttttcaa tcctgtatat gaactaatgg caaagataac cgtgattgtt aataaaatgg
    27601 ccgaacgtaa aatcatattt ttaccatcta aaccagttaa tctttctatg cctgcagaaa
    27661 ttaatggcac acctaacgct agtgatgttg ccgcaccacc tagcaatcca aagataaata
    27721 agatatctac aactttacct acaaatttat ctgtttgacc ttttaaaatc ggacgacaag
    27781 cttgactaat tttatacacc ggttgttttt taacaaatac taaataacca attggtaatg
    27841 ctggtaaaac gtaaatagcc caagcaattg gcccccagtg gaacatacca tattgcgtcg
    27901 catattggag tgcttcatca ctcatacttt tcgcgccatt tggtggaact tgatagtaaa
    27961 aagcccattc aataacgccc cagtataaaa tatcagagcc tatgcctgca caaaacagca
    28021 ttgccgccca tgtaaatgta ttaaattctg gtttatcact tgctttacca agtgtgacat
    28081 taccatattt accaaatgcg atatacatta caaagcaaaa aatcgccagc cccataaata
    28141 aatatatcga accaattgaa tcagaaatgg cactattaat accagtgatg atatcttcac
    28201 ttgcttttgg aaaagccatc ataggtataa ctgcaaaaag aagtacagct actgtcccta
    28261 taaaggtcgt ccagtccata actttctctt ttttcaattg tgctccccct aattattaat
    28321 tttatgaatc ctgtttcgat ttatctcaaa atgtaataat tatattgatt cacaaaattg
    28381 acaataacta acatttaata ataatgcaaa tttcatacaa tttgaaactt ggcaattatt
    28441 gaatatttat ataatttttc ccgtaataaa caaaacctta atagcgctaa aacaatagtg
    28501 ttaagttatg atttaacgaa tttaacaaat tttactagga cggcatttaa gaatatttat
    28561 acgttattaa cgaatattta tttattgtaa aacgctacca aaaagttaga cttccttccc
    28621 actaaaatac cacttttctc tttcaacttt tttaaaaaac ggatatgcaa cttttagtat
    28681 tggtatcaaa atgattgtta ggtcatattc tatcaatata tttttctaaa gcattgcttt
    28741 tattaacttt caattatgta cctaacctaa aaagaagcca aggcaacgaa tgttaccttg
    28801 acttctaata catattcaac taactatata ttcaatcata cgcgcatgcg agagtgattg
    28861 ttgtacatct ataatgcgtt gatttaaaga acctttatat ggtaaatcag gtttgaataa
    28921 gtgttgtata aatagaccat ctactaaaac gtcaatgtat gataataact ctcgacgttc
    28981 tgtacaatca tttgctaaat attcatataa aaatccagtc catacccaaa ttgtctttgt
    29041 atttccaaaa cgtgctcgaa atgctttgac aagatttaat gtaatatcca aattacaaaa
    29101 tggttcgcca cctaatagac ttagcccaga tatataatca tgatcgcaat catctaatat
    29161 ttctgctaat atttcatcag tgtatttctc gccatatctg aacttttgtg aggctttgtt
    29221 ataacatcca acacaattaa atggacatcc tgatacataa acactgcatc ttactccttc
    29281 accgtcaaca aagctatttg attctatttt agcaatataa ccttgtcctt gtttaatgtc
    29341 taaaagtgtc attctttagg cgctttcata tgttttactc gtgcgcaaat ttctttatga
    29401 cggcctttaa ttactggacg ttgaactgga ttgcctaagt aaccacatgt tcgtttaacg
    29461 acatcaactg ttttaggatt atcattgcca cagttcgggc atttaaatcc tttttcagtt
    29521 gcttcaaaat ctccatcgta atcacattca taacaatgat caatcggaat atttgtacct
    29581 aagtaaccaa ctttgtcata agagtagtcc cataccgctt ctagtgcttt caaattgtgt
    29641 tgcaatttcg gatactcaca atagtgaatg aaaccaccac tcgcataata aggataatct
    29701 ttttcaaaat ctaacttttc aaaaggtgta acatctttac gtacatcata atggaaagag
    29761 ttttgataat atcctttatc tgtaatgtct ttaatatctc caaatctctc ttggtctaaa
    29821 cgacaaaaac gatccgttag cgattcactc ggcgtactgt aaatactgaa ccaaatgtca
    29881 tataattctg tccatttcgt ttgataacgt ttcatttctt taagaatttc aagcgtaaat
    29941 gcttttgctt cttgagatgt ttcccagtct ggaccataga aaacagtagc tgtttcatac
    30001 aaccctatat agcccattga aatcgttgca cgtttatttt taaataactc agcaacatca
    30061 tctgtttctt ttaatttata gttaaatgcg ccacttttat ataaaattgg tgcgttattc
    30121 ggtacagcat ctttcaaacg atttatacga taaagtaatg catcatgtaa cacatcgata
    30181 cgttcataaa aaatttccca gaatttcgtc atattaccgg cagattctaa tgccattcta
    30241 ggtaaattaa gtgtaacaac accaagatta caacgaccat tattttcaaa atgtcctgcc
    30301 gcatctttcc aacttggtaa aaatgagcga caacccattg gcgctttgaa atcacctaat
    30361 atttctacga gtttgtcata atttaaaata tctgggtaca tacgtttcgt tgaacacttt
    30421 aatgctagtt gtttaatgtc atagttcgga tcttgcggac taaagttggt tcctttttta
    30481 attgaaaata caagtttcgg gaaaatcgct gtcgtgcggt cttttcctaa gcctttgata
    30541 cgagtattta agatagcttg ttgaattttg cgacttaaat gatctgtacc taagccgaat
    30601 cctaatgtta caaaaggtgt ctgtccatta gatgtatata aggtattaat ttcatattct
    30661 aaactttcaa tcgcatcatt gatgtcttta gtgacttgtt gatcaacata acgatcaatt
    30721 tcagattctt tgacaaattg ctttgcgata ttcctatgtt gttcttcatt atgtcgtgca
    30781 tatgtactaa gtaattcgtc aacgcggtca accgtacagc caccatattg actgctggaa
    30841 acattggcta taatttgtac aagctgcgct gatgcagttt gtattgattt tggtgaagtt
    30901 acattcgcgt tgcctatttc aaatccatta tgtagcatat ttttagcatc tattaaacaa
    30961 cagttagtta acggttggaa tggatgataa tctaagtcgt gaaaatgtat gtcccctctt
    31021 tgatgtgcat ctgcaacgtg ctttggtaat aaatgattta atgcgtaaga tttagaaacg
    31081 atacctgctg ttaaatctct cattgttgaa aatgtatcac tatctttgtt agcattttcg
    31141 ttaacaatag caggatcttt agaaatcaaa cccgttaatg cttcttcgat ttgattcatt
    31201 ttaccccttc ttcctatata ttgtgtctac tcattacgtt ataccactat atatagtatt
    31261 ttcaaatttt tattagtctg ttaactaaaa cgtcacaaag ttattaacat ttagtgaaat
    31321 tcatatttct acacaattat ttcattaatt tttaaaattg tttaaagatt cgtccgtcat
    31381 ttcgacatgt ttatgcaact attatttcat tttgcaccgg gactatagtt tctatttttc
    31441 atacttaaat cgtcacaatg cccatcaaca ttgcaattac taacataacg atggcgaatc
    31501 cccaaatcca aaagaatgca tacttaatat acgtgcccat gtttgcctct gctaaaccaa
    31561 ttgccaacca taaggctggt gaaaacggac tgacaaatgt acctataata ttccctataa
    31621 ccattgaata agctgttgat acagacggta caccaaattg ccctgctgtt tgttcgacga
    31681 tcggtaacac agcaaaataa taagcgtctg tgctagttag taaatctaat ggtacgccaa
    31741 gtaaacctac aataatatgc aagtatggtc ctacttctgc aggaatcact ttgattaaat
    31801 tcgtcgcaat cgctttaagc ataccggttt catttagtac acctaaaaac atacctgctg
    31861 caataatcac tgcagccatc attaatgcat tcggcgcatg cgctcttaat cgctccattt
    31921 gttcatccac tgatttaaaa ttaataacaa gcgccaatga gacacctatc atgaatgcaa
    31981 attcaggtgg cgcaatattt attaacatcg ctagaataac agctaaagtt aaagctgtat
    32041 tcacccattt tatccatggt tttgttcgtg cacgtccttt tacaggaaac cttacatctt
    32101 gatctcgttc atatacttca actaatttat gtacatctat atcttgtgtt tgcggtaatt
    32161 catttctctc tattgctttt ttgatacgtt tttgttcttt aaatccaaga tataccgcaa
    32221 acaacataac aagaatgaat cctattattt gaataggaat taatccatac cataattcat
    32281 tgacactttt ggcttttaac actgcagcta cacgtgccat tggacctccc caaggtacca
    32341 tgttcataat cgccgcactt aatgctaata gtaaaatcaa taaatattta ttcatattta
    32401 acgctttata taaaggtaat aatgcaggaa tagaaagcaa aaatgttacc gcaccggccc
    32461 catctaattg ggctattgtg ccaattaaag ctgtcattgc acagacaatg acgacattgc
    32521 ctcgtgtcat taatattaag cgtttgacaa gcggcttgaa taaaccacta tcgttcatga
    32581 taccaaagaa aataatggca aagataaaca taataacaac gttgatgact tgatctaacc
    32641 ctttagcaaa aaatccaacc aaatctgtca cactatatcc taaaatcatt gcccctaagc
    32701 aaggtatgat tgtcatacct acaactggat ttatcttttt ggcaatgagt aaacctacaa
    32761 ttgaaataat aataatgagc cccattactg ttaacaacat attatcacta ttcatattat
    32821 tttctccctc cacttttaat acaatttaca taatccccct ctttaactta aatattataa
    32881 ttaatataat tttttatatt taagttatct cattttatca aaaaaatctt ttttgcgtaa
    32941 ctatcaatag taaagtttga ttttcctact tgataacatg gcatgactaa tccttgtata
    33001 tcctgtcctg tatttcattt tgatttatca tttttattat tgctttctat tgcacaaaaa
    33061 atctggctaa aggtcgctat gatagtaacg acttcaacca gatttcaatt tatccatcta
    33121 ttttattaag tttgttctac gtatcttttt ctctattgta atattcatat ttaaaacgca
    33181 tatcattatc gcttaaatcc ttcattatta gactattcac aaagccgccc atgtcttaat
    33241 ttcaatgatt aagcgttaaa gttaaaataa gaagacttac cttagacggt tagacggtcc
    33301 gctattatta tcttacatcc aaccacgcta aaaattgagc ttgtttgtca tgatttaaat
    33361 attcttgtga cacaatttgt gacagtaact gttgcttttc gttatatgtt atatcaagta
    33421 cttttatttt ctgtcggcaa gtatataaaa agtcgatata cgaactgtat gatggtggat
    33481 ataacgcctc aagctctgcc ataattgatt ttgtcaactt aggactcgca ccatcagttg
    33541 atacactgat agttagcttg tcgcggtgta gtgcacttgg aaatacaaca ttgccatttg
    33601 atgcatctcc aacattatta aaaagggcat gctcaggtaa ggcttgtttt accgcttcat
    33661 tgacacgtgg ctcattggtt gctgcaataa ctaaataagc atttacaata tcacttggtt
    33721 caaattcttt ttctttccat atgacgacac ctttatctac cattttttga agtttttctg
    33781 tgattgtcgg actgatgacc gtcatatgtt caacgtattg acttaatgtt tgtgcccgac
    33841 gacttgcaat gacgcctcca ccaactatga cgacattttt atttgttaaa tctatcatta
    33901 atggcatatt catgttgagc aacatccttt atatcttttt acatcattac accttaaaat
    33961 atttaacacc aatcgctaat aaacatcacg ttgatagcgt tgttgttgtt tcatttgtcg
    34021 caataataac tctgcttctt cttgagaaat atgacgttct tttaccaata catctttaat
    34081 ggcttgatgg acatctttcg ccatacattt ttcatcgcca caaatataga ttgctgcgcc
    34141 ttgctcaatc cattcattga aacgttcact ttcttccatt atacgatgct gtacatattc
    34201 tttgtgttct tggtctcttg aaaatgctaa atctacgcgt gttaagtttc cattttcaag
    34261 ccattcttct atttcttctt catataaaaa gtcagaacta cggtgttgat caccaaagaa
    34321 caaccatgtt tttccagtca taccaagttc ttcacgttct tgtaaatatg ctctaaaagg
    34381 agcaattcca gtacctggtc caatcataat aaccggtata tcttgcttca tcggaaattt
    34441 gaagttcgga tttttcttca aataaattgg tactatatcg cctggtttaa tacgctcagc
    34501 aaaatgaacc gagcatacac cttttctctc acgtccatgt gcttgataac gaaccgtacc
    34561 aacggtaata tgcacttcat ctggcgttgc cataaaacta ctagaaatcg aatactctct
    34621 tggtggtaat tttctaagga tttgatacat attctcaggt tgtaattcta tagttggaaa
    34681 atctgttatt aaatctataa agtcccgatt tataacatat tcacgcgccc atgactcatc
    34741 ttgaatacgt tcagataatt cttcattgtc aaaatagata tctgcatttt tcaataatgg
    34801 caatgttaat ttagtaaatt caaaatgtga tgttagagct tcaacaatag gaactgtatc
    34861 accatgatca ttaattggca ccggagattg cggatcccaa cctaacatgg atattagttt
    34921 ttcaaccaat tcagggtcgt tttgcggtaa tgctactata caatctcctg gttcatatga
    34981 ttcactaaaa tcatcaagta aaaattctat atgtcgtgtt tctttatttg aatcggtacc
    35041 atttaaattg atattcgcta atacttctgc ttggtatgga tttgatttag aatatttctt
    35101 ttctttggca gacttaattg attcacttat caccgattca ctttgaatac cttctgatgt
    35161 ggtatcaatc atattaatga tgtctgccat ccacttttct gcgtcttctt cataatcaat
    35221 atcacaatct acacgcttac atatatgttc agcgcctaga ttttctagta aagcatcaac
    35281 atctttacca gcttgacaga aaaattcata tgtttgatca cctaaagcta gtactgaata
    35341 tctcacatga tttaaattag gtgcgttatc gtcttctaaa aattcaaaga aatcccatgc
    35401 attatcaggc ggttctcctt caccatgagt agacgtaata ataaataaat cttctaactg
    35461 tgcgatgttt gtcgtatcat attcatccat tgacatcaaa acaacttgat gtccgatatc
    35521 acttaaacgt tctgaaaaaa tttcagctaa acgcatggca ttacctgatt cagaaccata
    35581 tagcactgta acatgccgct gattagcttc gatatgtggc tctttatttt gtaacatata
    35641 cgttgttgaa gttgcttgtt cttctgatat ttgtgattgt tgccacactt ctgtctctaa
    35701 ttgttgttga ttactatcac ttgtcgtatc gttactatta gctagtaaaa acccactaag
    35761 ccactgttgt tggctctctg ttaatgtttg cagcagattg ttaatctgtg agacttgttt
    35821 ttctgtaaat ggactattag atgtatttaa tttcaaatat tttcacctca ataaacttga
    35881 aatagatgat tgactcattt tttatcgata gtttagtcaa ttttttaata tgtatggtat
    35941 aaattaaaac aaacttgagt tgtgtctgat agttatactt gcaaatattt acaactttca
    36001 tgttgcttga atgacattat cagtaattca gacaaaaata tgattatgtc gtatcagtta
    36061 ttaatgcata cagtaatgca tcgttgttat tttttattta tttactattt attatgtacc
    36121 aaatcagcct ttcagtaaaa taggtcaaat tactgatttt ctaaatataa aatgcctcct
    36181 aataacatac tattagtaca tcattaagag gctcttgtgt tatttgcata ctaagcgctc
    36241 aaatttaaat ttaagatgaa gattcttgca ataatatttc tatatttgtc gatatatcca
    36301 atggatcttc cattggcaag aatcgattta caacatttcc ttgtcgatcg attataaatt
    36361 ttgtgaaatt ccattttatt ggggacccaa agattcctgg ttgttcattc tttaaatgcg
    36421 taaataacgg atgttcatcg ttcccgttca cagatatttt agctaacact ggaaatgtta
    36481 caccaaattt ctcacgacta attttcaaga tttcttcatt agaacctggt tgtcgattgt
    36541 caaaattatt attcggaaaa ctcaacacta caaacccacg atccttatat ttttgaaaca
    36601 aagtctctag ttttttcaat tgttcgctat atatacattc tgttgcagta ttaacaacca
    36661 aaatcacttt acctctaaat gcttctaatt tataagttaa gcctttataa tcacttactt
    36721 cgatatcata cacatttcta ttattcataa gacaccccta cacagccttt tttatattga
    36781 atatgttctt tttagaatgt tctgataaaa taagtgcgcg tttacaccgc gaacatacat
    36841 tatatagcgt gatacatttt tcgagcacac gataaataat gttcgagttt atgttgttgc
    36901 tcaacctatc cgatttaccg tcttttttca cctgtcttac tttattaaaa taatcacatt
    36961 gtatagctgt attagtccag ctacaatatc tttcgttata acccctcatt ataatcatcc
    37021 ttattttcta tttttaaaaa gacaattaga ccgctcttta aactatagat taatacttaa
    37081 gttaaactca tacatactga taccatacgt tagatctaac aatttaaaat tcgttataac
    37141 tatggattaa agagctgccc aactcatata atccttaaaa acttcacatg tgattgttta
    37201 ttaagccctc ctttatctta ttaaatatcc ttataaccct tttaaaatta aactgacaca
    37261 ctcatacatt gttacacgcg acgataaacg tcatattatt gcatatcatt aaaacaagtc
    37321 actttgtatc gtgtgcgacc gaatacatat taaatcatca ataatgatta ttcaattatt
    37381 aaaaataaaa acgaacattc tatatagagt ttctacttat tgttttttca gcattgtcta
    37441 cattacttaa cattcgtgtt tgtaagtaat attgaccgcc aatatttaga cactttataa
    37501 gtatgccatt catcattttt aatcatatta tttttcttat tgatatttct attcatatat
    37561 ttctgattgt atatttagat ataataattt gttattggaa attatatgaa acaaatttga
    37621 ctcatctttt tcaatatcta aatgacacga tgcatgattt ttgaattttg taagtaactt
    37681 ccttttaaat tagactgaat tgtgacaatt ttcaaacatt ttaatgtgta aatcattggc
    37741 aaaatatacg aattgcattt aaatcgtcaa tttacatata tttttgcgta gtaatcaatc
    37801 gttatcattg ttatttatcg ttacattact tcgagtatca gtatgtattt cgggcttcgt
    37861 ttgataacga catttctttg tgacatcgct tcatcagtgt aacaacaaat acaatgattt
    37921 cgtgatgtta gttacccatt ttatgtgttg cataaaatat gttgttataa aacattttaa
    37981 atcattttat ataaacaatc tatatatttt tgttatcccc aaaatatcac ttgttatatt
    38041 aaaaaccgac aagacatttt atcttatcgg ttcaaatttg gtattgttat ttgtaatgtt
    38101 tttaggtttc
//
LOCUS       DAHHYJ010000009        35471 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000009 DAHHYJ010000000
VERSION     DAHHYJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 35471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.009"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            131..1249
                     /gene="ald"
                     /locus_tag="NBK22_000371"
     CDS             131..1249
                     /gene="ald"
                     /locus_tag="NBK22_000371"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCD2413588.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSNDMYEKEGAKIVTHEQAWEADLVIKVKEPHESEYQYFKKNQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLEDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLASSHDLDYKEILNVIE"
     gene            1344..2384
                     /gene="tdcB"
                     /locus_tag="NBK22_000372"
     CDS             1344..2384
                     /gene="tdcB"
                     /locus_tag="NBK22_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCD2413589.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2415..3737
                     /locus_tag="NBK22_000373"
     CDS             2415..3737
                     /locus_tag="NBK22_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2413590.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3893..5284
                     /gene="norB"
                     /locus_tag="NBK22_000374"
     CDS             3893..5284
                     /gene="norB"
                     /locus_tag="NBK22_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCD2413591.1"
                     /translation="MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFFIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEILYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
     gene            5682..35471
                     /gene="ebh"
                     /locus_tag="NBK22_000375"
                     /pseudo
     CDS             5682..35471
                     /gene="ebh"
                     /locus_tag="NBK22_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635093.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
BASE COUNT        14111 a         6219 c         6436 g         8705 t
ORIGIN      
        1 ataatatatt aaatatttat taacaaatta tagataaaat atgaataatt aattaatggt
       61 atttacatat tcataaccaa aggataaaat aattgtgaaa ttattcacaa atgaaagagg
      121 agtgttgcaa atgttagttg cagtagtcaa agaattaaaa caaggtgaag gacgtgtagc
      181 ttgcacaccc gaaaatgtgc gtaagttaac ggatgcagga cataaagtaa ttgttgaaaa
      241 aaatgctggc attggttcag gattttctaa cgatatgtat gaaaaagaag gcgctaagat
      301 cgtaactcac gaacaagcat gggaagctga tcttgttatc aaagtaaaag aacctcatga
      361 aagcgaatat caatatttca aaaagaatca aattatctgg ggatttttac atctagcatc
      421 ttcaaaagaa atagtagaaa aaatgcaaga agttggtgta actgcgatta gtggtgaaac
      481 cattataaaa aatggaaaag cagaattatt agcgccaatg agtgctatag caggtcaacg
      541 ctcagcaatt atgggagctt actactctga agcacaacat ggtggtcaag gtactttagt
      601 gactggtgta catgaaaatg tggatatacc tggtagtaca tatgtgattt tcggtggtgg
      661 agtagcagca acaaatgcag caaatgttgc cttgggacta aatgctaaag taatcattat
      721 cgagttaaac gatgaccgca ttaaatatct tgaagatatg tatgcagaaa aagatgtcac
      781 agtagtcaaa tcaacaccag aaaatttagc agaacaaatt aagaaagcag atgtatttat
      841 ttctacaatt ttaattccag gtgcgaaacc gccaaaattg gttactcgtg agatggttaa
      901 atcaatgaaa aaaggttcag tattaatcga tatagctatt gaccaaggtg gaactattga
      961 aacaattaga ccaactacaa tttctgatcc agtgtatgaa gaggaaggtg tgattcatta
     1021 tggtgtacca aatcaaccag gagcagtccc aagaacttca acaatggcat tagcacaagg
     1081 aaatattgat tatatattag aaatttgtga caaaggctta gaacaagcaa ttaaagataa
     1141 tgaagcctta agtactggtg taaacattta ccaaggacaa gtgacaaatc aaggattagc
     1201 ttcatcacat gacctagatt ataaagaaat attaaatgtt atcgaataga tagtaattta
     1261 aatgaaattg agtgaaatga atattttaaa tatagcatta tagtttggac taaaaattta
     1321 caaaacggaa ggatgtaatt attatgacaa ccaacacagt tacattacaa acagcacata
     1381 ttgtaagttt aggagatatc gaagaagcta aagcaagcat taaaccattt attcgtcgaa
     1441 cacctctaat taaatcaatg tatttaagcc aaagtataac taaagggaat gtatttctaa
     1501 aattagaaaa tatgcaattc acaggatctt ttaaatttag aggcgctagc aataaaatta
     1561 atcacttaac agatgaacaa aaagaaaaag gcattatcgc agcatctgct gggaaccatg
     1621 cacaaggtgt tgctttaaca gctaaattat taggcattga tgcaacgatt gtaatgcctg
     1681 aaacagcacc acaagcgaaa caacaagcaa caaaaggcta tggggcaaag gttattttaa
     1741 aaggtaaaaa ctttaacgaa actagacttt atatggaaga attagcgaaa gaaaatggca
     1801 tgacaatcgt tcatccatat gacgataagt ttgtaatggc aggccaagga acaattggtt
     1861 tagaaatttt agatgatatt tggaatgtga atacagtcat cgtaccagtt ggcggtggag
     1921 gattaattgc aggtattgcc accgcattaa aatcatttaa cccttcaatt catattatcg
     1981 gtgttcaatc tgagaatgtt catggtatgg ctgagtcttt ctataagaga gatttaactg
     2041 aacatcgagt ggatagcaca atagcagatg gttgtgatgt aaaagttcct ggtgaacaaa
     2101 catatgaagt agttaaacat ttagtagatg aatttattct tgttactgaa gaagaaattg
     2161 aacatgctat gaaagattta atgcagcgtg ccaaaattat tactgaaggt gcaggcgcat
     2221 taccaacagc tgcaatttta agtggaaaaa taaacaataa atggcttgaa gataaaaatg
     2281 ttgttgcatt agtttcaggc gggaatgttg acttaactag agtttcaggt gtcattgaac
     2341 atggactgaa tattgcagat acaagcaagg gtgtggtagg ttaaaacatt taatcttaaa
     2401 aatgaggtgt aattatgtca aatggtaaag aattacaaaa aaatataggt ttcttctcag
     2461 cgtttgctat tgttatgggg acagttattg gttcaggagt attctttaaa atatcaaacg
     2521 taacagaagt aacaggaaca gcaggaatgg ccttgtttgt atggttccta ggcggcatca
     2581 ttaccatttg tgcggggtta acagcagcag aacttgctgc tgcaatccct gaaacaggtg
     2641 gcttaacgaa gtatatagaa tatacatacg gtgatttctg gggcttccta tcaggttggg
     2701 cgcaatcatt tatttatttt ccagctaacg tagcagcatt gtctatcgta tttgcgacac
     2761 agctaattaa tttattccat ttatctatag gttcgttaat accaatagca atcgcatctg
     2821 cgttatctat tgtgttgata aatttcctag gttcaaaagc aggcggaatt ttacaatcag
     2881 ttactttagt aattaaactg attccaatca tcgttattgt aatttttggt atttttcaat
     2941 ctggagatat cactttttca ttaattccaa ctacaggtaa ttcaggaaat ggcttcttta
     3001 cagcaattgg tagtggttta ttagcaacta tgtttgcata tgatggttgg attcatgtag
     3061 gaaatgttgc gggggaactt aaaaatccta aacgcgattt acctttagcg atttcagttg
     3121 gtatcggttg tattatggct gtgtatttat taattaacgc tacattctta ttaacgttgc
     3181 caatagaact acttgctggt aatttaaatg cagcttcaga tacatcaaaa atattatttg
     3241 gtgaaaatgg cggtaagatt attacaatcg gtatattaat ttctgtttat ggtacgatca
     3301 atggctatac tatgactggt atgcgcgtac catatgcaat ggctgaaaga aaattattgc
     3361 catttagcca tttattcgca aaattaacaa aatctggcgc accatggttt ggcgcaatta
     3421 tacaacttat aatcgctatc atcatgatgt caatgggagc atttgataca attacaaaca
     3481 tgttaatctt tgttatttgg ttgttctatt gtatgtcatt tgttgcggta ataattttaa
     3541 gaaaacgtga accaaatatg gagcgtccat ataaagtacc gttatatccg atcatacctt
     3601 taattgctat tttggcagga tcttttgtat taattaatac actgtttaca caatttatat
     3661 tagcaatcat tggaattcta ataacagcac ttggtatacc agtttattac tataagaaga
     3721 aacaaaaagc agcataaggt aagataacta gcattgagaa taaatggatg gactactaat
     3781 aaatttaaag ttttacacat taaaattaaa accattcact tattctaacg aacagacaaa
     3841 tttctgttat ggaatttgtc tgtttttcaa aagtataggg aggcaaatag agatggaaaa
     3901 gccgtcaaga gaggcatttg aaggcaataa taagttgtta ataggaattg ttttaagtgt
     3961 aataacgttt tggctatttg cacaatcatt ggttaatgtt gtaccaatac ttgaagatag
     4021 tttcaataca gatattggaa cggttaatat cgccgttagt ataactgctt tattttcagg
     4081 aatgtttgta gtaggagcag gtggtcttgc tgataaatat ggcagaatta aactcacgaa
     4141 cattggtatt atcttaaata tattaggttc attattaatc attatttcaa atattccttt
     4201 attacttatt ataggaagat taattcaagg actttcagca gcatgtatta tgcctgcaac
     4261 tttgtctatt attaagtcat attacattgg gaaagataga caacgcgctt taagttattg
     4321 gtcaattggc tcatggggcg gctctggtgt ttgttcattt tttggaggtg cagttgcaac
     4381 gcttttaggt tggcgttgga ttttcatcct atcaattata atttcattaa ttgcactgtt
     4441 ttttattaaa ggcacacctg aaactaaatc taaatcgatt tctctaaata aatttgacat
     4501 taaaggtctg gttcttttag tcattatgct cctcagttta aatattttaa ttactaaagg
     4561 atcagaatta ggtgtaacct cacttctttt tattacttta ttagctattg caattggatc
     4621 ttttagttta tttatagttc ttgaaaagcg tgctacaaat cctttaatcg attttaaatt
     4681 atttaaaaat aaagcttaca caggtgcaac agcttcaaac tttttgttaa atggtgttgc
     4741 aggaacatta atagtagcca acacatttgt tcaaagaggt ttaggatatt cttcattgca
     4801 agcaggaagt ttatcaatca cttatttagt aatggtacta attatgattc gtgttggtga
     4861 aaagttactt caaacactcg gatgcaagaa accaatgtta attggaacag gagttcttat
     4921 tgtcggagaa tgtctcattt cattaacttt cttgccagaa atattgtatg tcatttgttg
     4981 tattataggt tatttattct ttggtttagg actagggata tatgctacac catcaacaga
     5041 tacagcaatt gcaaatgcac cgttagaaaa agtaggcgtt gctgcaggta tctataaaat
     5101 ggcttctgca ttaggtggag catttggcgt cgcattgagt ggtgcagtat atgcaatcgt
     5161 atcaaatatg acaaacattt atacaggtgc aatgattgca ttatggttaa atgcaggtat
     5221 gggaatatta tcattcgtta tcattttgtt acttgtgcct aaacaaaacg acactcaatt
     5281 atgataattg agaattaaat tgaaatcata caagtcgcta caatattaaa caaaaatata
     5341 aaccgattct tatgtgtcat tatttaaaat gaacataggg attggttttt tattactctt
     5401 ttacgctact ttatttataa ttattataaa ttgtcacaaa ttcaatttac tatacaatat
     5461 attttgtgtt attatattct ggatcataaa taaattgttc aacacatagt tgtaatgtgt
     5521 ttcaatactt tttggataga ttgcgaaatt gtattgaatc gtcatcgttt taaattttta
     5581 aatgagaatg gaatgagcat tacaatatac aagcaatcaa aagtaaatac attcacaaca
     5641 caacagagac ataacaacaa gataaggagt gaacaatagc tgtgaattat cgtgataaaa
     5701 ttcaaaagtt tagtattcgt aaatatacag ttggtacatt ttcaactgtc attgcgacat
     5761 tggtattttt aggattcaat acatcacaag cacatgctgc tgaaacaaat caaccagcaa
     5821 gcgtggttaa acagaaacaa caaagtaata atgaacagac tgagaatcga gaatctcaag
     5881 tacaaaattc tcaaaattca caaaatggtc aatcattatc tgctactcat gaaaatgagc
     5941 aaccaaatat tagtcaagct aatttagtag atcaaaaagt agcgcaatca tctactacta
     6001 atgatgaaca accagcatct caaaatgtaa atacaaagaa agattcggca acggctgcga
     6061 caacacaacc agataaagaa caaagtaagc ataaacaaaa cgaaagtcaa tctgctaata
     6121 aaaatggaaa cgacaataga gcggctcatg tagaaaatca tgaagcaaat gtagtaacag
     6181 cttcagattc atctgataat ggtaacgtac aacatgaccg aaatgaatta caagcatttt
     6241 ttgatgcaaa ttatcatgat tatcgcttta ttgaccgtga aaatgcagat tctggcacat
     6301 ttaactatgt aaaaggcatt tttgataaga ttaatacgtt attaggcagt aatgatccaa
     6361 taaacaataa agacttgcaa cttgcataca aagaattgga acaagctgtt gctttaattc
     6421 gtacaatgcc tcaacgtcaa cagactagcc gacgttcaaa tagaattcaa acgcgttcgg
     6481 ttgaatcaag agctgcagag cctagatcag tatcagacta tcaaaatgca aattcatcat
     6541 attatgttga aaatgctaat gatggttcgg gctatcctgt tggtacatat atcaatgctt
     6601 ctagtaaagg ggcgccatat aatttaccaa ctacaccatg gaatacattg aaggcctctg
     6661 actcaaagga aattgctctt atgacagcga aacaaactgg agacgggtac caatgggtta
     6721 ttaagtttaa taaaggacat gctccacatc aaaatatgat tttctggttt gcattaccag
     6781 cagaccaagt gccagtagga agaactgact ttgtaacagt taattcagat ggaacaaatg
     6841 tacaatggag tcatggagca ggagcaggtg caaataaacc acttcaacaa atgtgggaat
     6901 atggagtaaa tgatcctcat cgttcacatg actttaaaat aagaaataga agtggccaag
     6961 taatatatga ctggccaact gtccatattt attctttaga agatttatct agagcgagtg
     7021 attattttag tgaagctgga gcaacagctg ctactaaagc atttggtaga caaaattttg
     7081 aatatattaa tggtcaaaaa cctgctgaat caccgggtgt tcctaaagtt tatactttca
     7141 tcggtcaagg tgatgcaagt tatacaattt catttaaaac acaaggtcca actgttaata
     7201 aattgtatta tgcagcaggt gggcgtgctt tagagtacaa tcaattattt atgtacagtc
     7261 aactatacgt cgaatcaacg caagaccatc aacaacgtct taatggttta agacaagtgg
     7321 ttaatcgtac atatcgcata ggtacaacta aacgtgtaga agtgagtcaa ggaaatgtac
     7381 aaacgaaaaa ggtattagaa agtacaaacc taaatataga tgattttgtt gatgatcctt
     7441 taagttatgt taagacgccg agtaataaag tgttaggttt ttacccaact aatgcaaata
     7501 ctaacgcttt tagaccgggg ggcgttcaag aattaaatga atatcaatta agtcaattat
     7561 ttactgatca aaaattacaa gaagcagcaa gaactagaaa cccaataaga ttaatgattg
     7621 gtttcgacta tcctgatggt tatggtaata gtgaaacttt agttcctgtt aacttaacgg
     7681 tattacctga aatccaacat aatattaaat tctttaaaaa tgacgatact caaaatattg
     7741 ctgaaaaacc attttcaaaa caagctgggc atccagtttt ctatgtatat gcaggtaacc
     7801 aagggaatgc ttccgtgaat ttaggtggta gcgtaacatc tattcaacca ttacgtatta
     7861 atttaacaag taatgagaat tttacagata aagattggca aattacaggt attccgcgta
     7921 cattacacat tgaaaactcg acaaatagaa ctaataatgc tagagaacgt aacattgaac
     7981 ttgttggtaa tttattacca ggggattact ttggtacgat acgttttgga cgtaaagaac
     8041 aattattcga aattcgtgtt aaaccacata caccaacaat tacaacgaca gctgagcaat
     8101 taagaggtac agcattacaa aaagtgcctg ttaatatttc gggaataccg ttggatccat
     8161 cggcattggt ttatttagtt gcaccaacaa atcaaactac gaatggtggt agtgaggcag
     8221 atcaaatacc atctggttat acgatacttg cgactggtac acctgatggg gtgcataata
     8281 caattactat acgaccgcaa gattatgttg tattcatacc acctgtaggt aaacaaatta
     8341 gagcagtagt ttattataat aaagtagttg catctaatat gagtaatgct gttactattt
     8401 tgccagatga cattccacca acaatcaata atcctgttgg aataaatgcc aaatactatc
     8461 gaggcgacga agtcaacttt acaatgggag tctctgatag acattctggt ataaaaaata
     8521 caactattac tactttgccg aatggttgga catcaaattt aactaaatcc gacaacaaaa
     8581 acggctcatt agctattaca ggtagagtga gtatgaatca ggcgtttaac agtgatatta
     8641 catttaaagt atcagcgaca gataatgtca ataatacgac aaatgatagt caatctaaac
     8701 atgtgtcaat tcatgtaggt aaaattagtg aagatgctca tccgattgta ttaggaaata
     8761 ctgagaaagt tgtagtagtc aatccgactg ctgtatctaa tgatgaaaag caaagcataa
     8821 ttactgcctt tatgaataaa aaccaaaata taagaggata tttagcatca actgatccag
     8881 taactgtcga taataatggt aatgtcacat tacattaccg tgatggctca tcaacaacgc
     8941 ttgatgctac aaatgtgatg acatacgaac cagttgtgaa atctgaatat caaactgcca
     9001 atgcagctaa aacagcaacg gtaacgattg ctaaaggaca atcatttaat attggtgata
     9061 ttaaacaata ttttacttta agtaatggac aagctattcc aagtggcaca tttacaaata
     9121 ttacatctga tagaactatt ccaactgcac aagaagttag tcaaatgaat gcaggcacgc
     9181 agttatatca catagttgct tcaaatgcat atcataaaga cactgaagat ttctatatta
     9241 gtttaaaaat cgttgatgtg aaacaacctg aaggcgatca acgtgtctat cgtacgtcaa
     9301 catatgattt aactactgat gaaatctcaa aagtaaaaca agcttttatt aatgcaaata
     9361 gagatgtaat tacacttgcc gaaggtgata tttcagttac aaatacacct aatggtgcta
     9421 atgtaagtac tattacagta aatattaata aaggtcgatt aacgaaatca ttcgcgtcta
     9481 acctagctaa tatgaatttc ttgcgttggg ttaatttccc acaagattat acagtgacat
     9541 ggacgaatgc aaaaattgca aacagaccaa cagatggtgg tttatcatgg tccgatgacc
     9601 ataaatcttt aatttatcgt tatgatgcta cattaggcac acaaattaca actaatgata
     9661 ttttaacaat gctaaaagcg actactacag tgcctggatt gcgtaataat attactggta
     9721 atgaaaaagc acaagcagaa gcaggtggaa gaccaaacta tagaacaact ggttattcac
     9781 aatcaaatgc gacaactgat ggtcaacgtc aatttacgtt gaatggtcaa gtgattcaag
     9841 tgttagatat cattaaccct tcaaatggtt atggaggaca acccgttaca aattcaaata
     9901 ctcgtgcaaa ccatagtaac tcaactgttg ttaacgtaaa cgaaccggca gctaatggtg
     9961 ctggcgcatt tacaattgac cacgttgtaa aaagtaattc tacacataat gcaagtgatg
    10021 cagtttataa agcacagtta tacttaacgc catatggtcc aaaacaatat gttgaacatt
    10081 taaatcaaaa tacaggaaat actactgacg ctattaacat ttattttgta ccaagtgact
    10141 tagtgaatcc tacaatttca gtaggtaatt acactaatca tcaagtgttc tcaggtgaaa
    10201 catttacaaa tactattaca gcgaatgata actttggtgt gcaatctgta actgtaccaa
    10261 atacatcaca aattacaggt actgttgata ataaccatca acatgtttct gcaacggcac
    10321 caaatgtgac atcagcaact aataagacaa tcaatttatt agcaactgat acaagcggca
    10381 atacagctac aacttcgttc aatgtaacag tgaaaccttt gcgtgataaa tatcgagttg
    10441 gtacttcatc aacggctgct aatcctgtga gaattgccaa tatttcgaat aatgcgacag
    10501 tatcacaagc tgatcaaaca gcaattatta attcattaac gtttactgaa acagtaccaa
    10561 atagaagtta tgcaagagca agtgcgaatg aaatcactag taaaacagtt agtaatgtca
    10621 gtcgtactgg aaataatgcc aatgttacag taactgctac ttatcaagat ggaacaacat
    10681 caacagtgac tgtacctgta aagcatgtca ttccagaaat cgttgcacat tcgcattaca
    10741 ctgtacaagg ccaagacttc ccagcaggta atggttctag tgcatcagat tactttaagt
    10801 tatctaatgg tagtgccatt ccagatgcga cgattacttg ggtaagtggt caagcgccaa
    10861 ataaagataa tacacgtatt ggtgaagata taacagtaac tgcacatatc ttaattgatg
    10921 gtgaaacaac gccgattacg aaaacagcaa catataaagt agtaagtaca gtaccgaagc
    10981 atgtctttga gacgaataga ggcgctgtgt tcccaggtgt atctgatgtg tatgatgcga
    11041 aacaatatgt taaaccagtt aatgattctt ggacacaaaa tgcgcaacgt atgaatttcc
    11101 aatttacaaa ttcatatgga ccaagtaaag atgtggttgg tatttcaaca agggatattc
    11161 gagttactta tgataatcac caaacgcaaa ttatcaagat tttagcaaaa gttaaaccag
    11221 atccaccgcg aattgatggc aactctgtga cttataaagc aggtcttact aaccaacaaa
    11281 ttaaaattaa taatgtgtta agtagttcat ctattaaatt atttaaagca gataatacgc
    11341 cattaacgat tacaaacacg acttatggta gtggtaatac tgcagttgta acggtaagtg
    11401 atgcgttacc aaatggtgtg attaaggcaa ggtcttctat cacaatgaat aatgtgacgt
    11461 acactactca agatgaacat ggtcgagcga tagatgtaac aagaaatgaa tctgttgatt
    11521 caaatgacag tgcaacagta acagtgacac cacaattaca agcaactact gaaggcgctg
    11581 tatttattaa aggtggcgac ggttttgatt tcggacacgt agaaagattt attcaaaacc
    11641 cgccacatgg ggcaacggtt gcatggcatg atagtccaga tacatggaag aatacagtcg
    11701 gtaacactca taaaactgcg gttgtaacat tacctagtgg tcaaggtacg cgtaatgttg
    11761 aagttccagt caaagtttat ccagttgcta atgctaaggc gccatcacgt gatgtgaaag
    11821 gtcaaaattt gacacatggt acaaacgcta ttgattacat tacatttgat ccaaatacta
    11881 atacgaatgg tattacagca gcatgggcaa atagacaaca accaaataac cagcaagcag
    11941 gcgttcaaca tttaaatgtc gatgtcacat atccaggtat ttcagctgct aaacgagttc
    12001 ctgtaactgt gaacgtatat caatttgaat tccctcaaac tacttataca acaacagttg
    12061 gtggcacttt agcaagtggt acgcaagcat caggatatgc acatatgcaa aacgctaatg
    12121 gtttaccaac agatggattt acgtataaat ggaataatgc agcaactggt acaaacgatg
    12181 caaactgggc agctatgaat aaacctaata tggctaaagt cgttaacgca aaatatgacg
    12241 tcatctataa cggacatact tttgcaacat ctttaccagc gaaatttgta gtaaaagatg
    12301 tgcaaccagc gaaaccaact gtgactgaaa cagcggcagg agcgattaca attgcacctg
    12361 gagcaaacca aacagtgaat acacatgccg gtaacgtaac gacgtatgct gataaattag
    12421 ttattaaacg taacggcagt gtcgtgacaa catttacacg tcgcaataat acaagtccat
    12481 gggtgaaaga agcatctgca gcaactgtag caggtattgc tggaactaat aatggtatta
    12541 ctgttgcagc aggtactttc aatcctgctg atacaattca agttgttgca acacaaggaa
    12601 gcggagagac agtaagtgat gagcaacgta gtgatgattt cacagttgtc gcaccacaac
    12661 cgaaccaagc gactactaaa atttggcaaa atggtcatat tgatatcaca cctaataacc
    12721 cgtctggaca tttaattaat ccaactcaag caatggatat tgcttacact gaaaaagtgg
    12781 gtaatggtgc agaacatagt aagacaatta atgttgttcg tggtcaaaat aatcaatgga
    12841 caattgcgaa taagcctgac tatgtaacgt tagatgcaca aactggtaaa gtgacgttca
    12901 atgccaatac tataaaacca aattcatcaa tcacaattac tccgaaagca ggtacaggtc
    12961 actcagtaag tagtaatcca agtacattaa ctgcaccggc agctcatact gtcaacacaa
    13021 ctgaaattgt gaaagattat ggttcaaatg taacagcagc tgaaattaac aatgcagttc
    13081 aagttgctaa taaacgtact gcaacgatta aaaatggcac agcaatgcct actaatttag
    13141 ctggtggtag cacaacgacg attcctgtga cagtaactta caatgatggt agtactgaag
    13201 aagtacaaga gtccattttc acaaaagcgg ataaacgtga gttaatcaca gctaaaaatc
    13261 atttagatga tccagtaagc actgaaggta aaaagccagg tacaattacg cagtacaata
    13321 atgcaatgca taatgcgcaa caacaaatca atactgcgaa aacagaagca caacaagtga
    13381 ttaataatga gcgtgcaaca ccacaacaag tttctgacgc actaactaaa gttcgtgcag
    13441 cacaaactaa gattgatcaa gctaaagcat tacttcaaaa taaagaagat aatagccaat
    13501 tagtaacgtc taaaaataac ttacaaagtt ctgtgaacca agtaccatca actgctggta
    13561 tgacgcaaca aagtattgat aactataatg cgaagaagcg tgaagcagaa actgaaataa
    13621 ctgcagctca acgtgttatt gacaatggcg atgcaactgc acaacaaatt tcagatgaaa
    13681 aacatcgtgt cgataacgca ttaacagcat taaaccaagc gaaacatgat ttaactgcag
    13741 atacacatgc cttagagcaa gcagtgcaac aattgaatcg cacaggtaca acgactggta
    13801 agaagccggc aagtattact gcttacaata attcgattcg tgcacttcaa agtgacttaa
    13861 caagtgctaa aaatagcgct aatgctatca ttcagaagcc aataagaaca gtgcaagagg
    13921 tacaatctgc gttaacaaat gtaaatcgtg tcaatgagcg attaacgcaa gcaattaatc
    13981 aattagtacc tttagctgat aatagtgctt taagaactgc taagacgaaa cttgatgaag
    14041 aaatcaataa atcagtaact actgatggta tgacacaatc atcaatccaa gcatatgaaa
    14101 atgctaaacg tgcgggtcaa acagaatcaa caaatgcaca aaatgttatt aacaatggtg
    14161 acgcgacaga ccaacaaatt gccgcagaaa aaacaaaagt agaagaaaaa tataatagct
    14221 taaaacaagc aattgctgga ttaacaccag acttggcacc attacaagct gcgaaaactc
    14281 agttacaaaa tgatattgat cagccaacga gtacgactgg tatgacaagc gcatctgttg
    14341 ctagatttaa tgaaaaactt tcagcagcta gaactaaaat tcaagtaatt gatcgcgtac
    14401 tggcttctca tccagatgtt gcaacgattc gtcaaaacgt gacagcagcg aatgctgcta
    14461 aaacagcact tgatcaagcg cgcaatggct taacagtcga taaagcgcct ttagaaaatg
    14521 cgaaaaatca actacaacat agtattgaca cgcaaacaag tacaactggt atgacgcaag
    14581 actctataaa tgcatacaat gcgaagttaa cagctgcacg taataagatt caacaaatca
    14641 atcaagtatt agctggttca ccaacagtag atcaaattaa tacaaatacg tctgcagcaa
    14701 atcaagctaa atctgatttg gatcatgcgc gtcaagcttt aacaccagat aaagcaccgc
    14761 ttcaaactgc gaaagcgcaa ttagaacaaa gcattaatca accaacagat acaacaggta
    14821 tgacgaccgc ttcgttaaat gcgtacaacc aaaaattaca agcagcgcgt caaaagttaa
    14881 ctgaaattaa tcaagtgttg aatggcaacc caactgtcca aaatatcaat gataaagtgg
    14941 cagaggcaaa ccaagctaag gatcaattaa atacagcacg tcaaggttta acattagata
    15001 gacagccagc tttaacaaca ttacatggtg catctaactt aaaccaagca caacaaaata
    15061 atttcacgca acaaattaat gctgctcaaa atcatcctgc gcttgaaaca attaagtcta
    15121 acattacagc tttaaatact gcgatgacta agctaaaagc cagtgttgcg gataataatt
    15181 cgattaaatc aggtcaaaat tacactgacg caactcaagc taataaacaa gcgtatgaca
    15241 atgcagtcaa tgcagctaaa ggtgtcattg gagaaactaa taatccaaca atggatgtta
    15301 atacagtgaa ccaaaaagca gcatctgtta aatcgacgaa agatgcttta gatggtcaac
    15361 aaaacttaca acgtgcgaaa acagaagcaa caaatgcgat tacgcatgca agtgatttaa
    15421 accaagcaca aaagaatgca ttaacacaac aagtgaatag tgcacaaaat gtacaagcag
    15481 taaatgatat taaacaaacg actcaaagct taaatactgc tatgacaggt ttaaaacgtg
    15541 gcgttgctaa tcataatcaa gtcgtacaaa gtgataatta tgtcaacgca gatactaata
    15601 agaaaaatga ttacaacaat gcatacaacc atgcgaatga cattattaat ggtaatgcac
    15661 aacatccagt tataacacca agtgatgtta acaatgcttt atcaaatgtc actagtaaag
    15721 aacatgcatt gaatggtgaa gctaagttaa atgctgcgaa acaagaagcg aacactgcat
    15781 taggtcaatt aaataacttg aataatgcgc aacgtcaaaa cttacaatcg caaattaata
    15841 gtgcacatca aatcgagaca gttaatacga ttaagcaaaa tgcaacaaac ttaaatagtg
    15901 caatgggtaa tttaagacat gctgttgcag ataaagagca agtcaaacgt acagaagatt
    15961 atgcggatgc agatacagct aaacaaaatg catataataa tgccgtttca agtgctgaaa
    16021 caatcattaa tcaatcaaca aatccaacga tgtctgttga tgatgttaat cgtgcaactt
    16081 cagctgttac tactaataaa aatgcattaa atggtgatga aaaattagca caatctaaaa
    16141 cagatgctgc atgagcaatt gatgcattac cacatttaaa taatgcacaa aaagcagatg
    16201 ttaaatctaa aattaatgct gcatcaaata ttgctggcgt aaatactgtt aaacaacaag
    16261 gtacagattt aaatacagcg atgggtaact tgcaaggtgc aatcaatgat gaacaaacga
    16321 cgcttaatag tcaaaactat caagatgcga cacctagtaa gaaaacagca tacacaaatg
    16381 cggtacaagc tgcgaaagat attttaaata aatcaaatgg tcaaaataaa acgaaagatc
    16441 aagttactga agcgatgaat caattgaatt cggctaaaaa taacttagat ggtacgcgtt
    16501 tattagatca agcgaagcaa acagcgaaac agcagttaaa taatatgacg catttaacaa
    16561 ctgcacaaaa aacgaattta acaaaccaaa ttaatagtgg tactactgtc gctggtgttc
    16621 atacggttca atcaaatgcc aatacattag atcaagccat gaatacgtta agacaaagta
    16681 ttgccaacaa agatgcgact aaagcaagtg aagattacgt agatgctaat aatgataagc
    16741 aaacagcata taacaacgca gtagctgctg ctgaaacgat tattaatgca aatagtaatc
    16801 cagaaatgaa tccaagtacg attacacaaa aagcagagca agtgaatagt tctaaaacgg
    16861 cacttaacgg tgatgaaaac ttagctacgg caaaacaaaa tgcgaaaacg tacttaaaca
    16921 cattaacgag tattacagat gctcaaaaga acaatttgat tagtcaaatt agtagtgcga
    16981 caagagttag tggtgttgat actgtaaaac aaaatgcaca acatctagat caagctatgg
    17041 ctagcttgca aagtggtatt aacaacgaat ctcaagttaa atcatctgag aaatatcgtg
    17101 atgctgatac aaataaacaa caagagtatg ataatgctat tactgcagcg aaagcgattt
    17161 taaataaatc gacaggtcca aacactgcgc aaaatgcagt tgaagcagca ttgcaacgtg
    17221 ttaatactgc gaaagatgca ttgaatggtg atgcaaaatt aattgcagct caaaacgcag
    17281 cgaaacaaca tttaggtact ttaacgcata tcactacagc acaacgcaat gatttaacaa
    17341 atcaaatttc acaagctaca aacttagctg gtgttgaatc agttaaacaa agtgcaaata
    17401 gtttagatgg cgcaatgggt aacttacaaa cggctatcaa cgataagtca ggaacattag
    17461 cgagccaaaa cttcttggat gctgatgagc aaaaacgtaa tgcttacaat caagctatat
    17521 cagctgccga aaccatttta aataaacaaa ctggaccgaa tacagcgaaa acagcggttg
    17581 aacaagcact taataatgtt aatagtgcga aacatgcatt aaatggtacg caaaacttaa
    17641 ataatgcgaa acaagcagcg attacagcaa tcaatggcgc atctgattta aatcaaaaac
    17701 aaaaagatgc attaaaagca caagctaatg gtgctcaacg cgtatctaat gcacaagatg
    17761 tacagcacaa tgcgactgaa ctgaacacgg caatgggcac attaaaacat gccatcgcag
    17821 ataagacgaa tacgttagca agcagtaaat atgttaatgc cgatagcact aaacaaaatg
    17881 cttacacaac taaagttacc aatgctgaac atattattag cggtacgcca acggttgtta
    17941 cgacaccttc agaagtaaca gctgcagcta atcaagtaaa cagcgcgaaa caagaattaa
    18001 atggtgacga aagattacgt gaagcaaaac aaaacgccaa tactgctatt gatgcattaa
    18061 cacaattaaa tacacctcaa aaagctaaat taaaagaaca agtgggacaa gccaatagat
    18121 tagaagacgt acaatctgtt caaacaaatg gacaagcatt gaacaatgca atgaaaggct
    18181 taagagatag tattgctaac gaaacaacag tcaaagcaag tcaaaactat acagacgcaa
    18241 gtccgaataa ccaatcaaca tataatagcg ctgtgtcaaa tgcgaaaggt atcattaatc
    18301 aaactaacaa tccgactatg gatgctagtg cgattaccca agctgcaaca caagtgaata
    18361 atgctaaaaa tggtttaaac ggtgctgaaa acttaagaaa tgcacaaaac actgctaagc
    18421 aaaacttaaa tacgttatca cacttaacaa ataaccaaaa atctgccatc tcatcacaaa
    18481 ttgaccgtgc aggtcatgtg agtgaggtaa cagctgctaa aaatgcagca actgagttaa
    18541 acacgcaaat gggcaacttg gaacaagcta tccatgatca aaacacagtt aaacaaggtg
    18601 ttaacttcac tgatgcagat aaagctaaac gtgatgcgta tacaaatgcg gtaagcagag
    18661 ctgaagcaat tctgaataaa acgcaaggtg caaatacgtc taaacaagat gttgaagcgg
    18721 ctattcaaaa tgtttcaagt gctaaaaatg cattgaatgg tgatcaaaac gttacaaatg
    18781 cgaagaatgc agctaaaaat gcattaaata acttaacgtc aattaataat gcacaaaaac
    18841 gtgacttaac aactaaaatt gatcaagcaa caactgtagc tggtgttgaa gctgtatcta
    18901 atacaggtac acaattgaat acagcgatgg ctaacttgca aaatggtatt aatgataaag
    18961 caaatacact agcaagtgaa aactatcatg atgctgattc agataagaaa actgcttata
    19021 ctcaagccgt tgcgaacgca gaaaatattt taaataaaaa tagtggatca aatttagata
    19081 aagctgccgt tgaaaacgcg ttgtcacaag ttgctaatgc gaaaggtgcc ctaaatggta
    19141 accataattt agagcaagct aaatcaaatg caaacactac tataaacgga cttcaacatt
    19201 taacaactgc tcaaaaagat aaattgaaac aacaagtgca acaagcacaa aatgttgcag
    19261 gtgtagatac tgttaaatca agtgccaaca cattaaatgg tgctatgggt acgttaagaa
    19321 atagcattca agataacaca gctacgaaaa atggccaaaa ctatcttgat gctacaggaa
    19381 gtaacaaaac aaactataac aatgctgttg atagtgctaa tggtgtcatt aatgcaacaa
    19441 gcaatccaaa tatggatgct aatgcaatta accaaatcgc tacacaagtg acatcaacga
    19501 aaaatgcatt agatggtaca cataatttaa cgcaagcgaa acaaacagca acaaatgcca
    19561 tcgatggtgc tactaactta aataaagcgc aaaaagatgc gttgaaagca caagtgacaa
    19621 gtgcgcaacg tgttgcaaat gtaacaagta tccaacaaac tgcaaatgaa cttaatacag
    19681 ctatgggtca attacaacat ggtattgatg atgaaaatgc aacaaaacaa actcaaaaat
    19741 atcgtgacgc tgaacaaagt aagaaaactg cttatgatca agctgtagct gctgcgaaag
    19801 caattttaaa taaacaaact ggatcaaatt cagataaagc agcagttgac cgtgcattac
    19861 aacaagtaac aagtacgaaa gatgcattga atggtgatgc taaactggca gaagcgaaag
    19921 cggcagctaa acaaaactta ggcactttaa accaaattac gaatgcacaa cgtactgact
    19981 tagaaggcca aatcaatcaa gcgacgactg ttgatggcgt taatactgta aaaacaaatg
    20041 ccaatacatt agacggcgca atgaatagct tacaaggtgc aatcaatgat aaagatgcga
    20101 cattaagaaa tcaaaattat cttgatgcag atgaatcaaa acgaaatgca tatacgcaag
    20161 ctgtcacagc ggctgaaggc attttaaata aacaaactgg tggtaacaca tctaaagcag
    20221 acgttgataa tgcattaaat gcagttacaa gagcgaaagc ggctttaaat ggtgctgaaa
    20281 acttaagaaa tgcgaaaact tcagcaacaa atacgattaa tggtttacca cacttaacac
    20341 aattacaaaa agacaacttg aagcatcaag ttgaacaagc gcaaaatgta gcaggtgtaa
    20401 atggtgttaa agataaaggt aataccttaa atactgccat gggtgcatta cgtacaagta
    20461 tccaaaatga taatacgacg aaaacaagtc aaaattatct tgatgcatct gatagcaaca
    20521 aaaataatta caatactgct gtaaataatg caaatggtgt tattaatgca acgaacaatc
    20581 caaatatgga tgctaatgcg attaatgaca tggcaaatca agtcaataca acaaaagcag
    20641 cgttaaatgg tgcacaaaac ttagctcaag ctaaaacaaa tgcgacgaac acaattaaca
    20701 acgcacatga cttaaaccaa aaacaaaaag atgcattaaa aacacaagtt aacaatgcac
    20761 aacgtgtatc tgatgcgaat aacgttcaac atacagttac tgaattgaac ggcgcgatga
    20821 cagcacttaa agcaactatt gcggataaag aaagaacaaa agcaagcggt aattatgtca
    20881 atgctgatca agaaaaacgt caagcttatg attcaaaagt gacgaacgct gaaaatatca
    20941 ttaatggtac accaaatgcg acattaacag tcaatgacgt aaatagtgcg gcatcacaag
    21001 tcaatgcggc taaaacagca ttaaatggtg ataacaactt acgtgtagcg aaagagcatg
    21061 ctaacaatac aattgacggc ttagcacaat tgaataatgt acaaaaagca aaattaaaag
    21121 aacaagttca aagtgcaact acattagatg gtgttcaaac tgttaaaaat agttctcaaa
    21181 cgttgaatac agcgatgaaa ggcttaagag atagtattgc gaatgaagca acaattaaag
    21241 caggtcaaaa ctacactgac gcaagtccaa ataatcgtaa cgagtacgac agcgcagtta
    21301 ctgcagcaaa agcaatcatt aatcaaacat cgaacccaac gatggaacca aatactatta
    21361 cgcaagcaac atcacaagtg acaactaaag aacatgcatt aaatggtgcg caaaacttag
    21421 ctcaagctaa gacaactgcg aaaaacaact taaataactt aacatcaatt aacaatgcac
    21481 aaaaagatgc gttaacgcgt agcattgatg gtgcaacaac agtagctggt gtaaatcaag
    21541 aaactgccaa agcaacagaa ttaaataacg caatgcatag tttacaaaac ggtatcaatg
    21601 atgaggcaca aactaaacaa actcagaaat acctagatgc agagccaagt aagaaatcag
    21661 cttatgatca agcagtaaat gcagcgaaag caattttaac aaaagctagt ggtcaaaatg
    21721 tagacaaagc agcagttgaa caagcattac aaaatgtaaa cagtacgaag acggcgttga
    21781 acggtgatgc gaaattaaat gaagcaaaag ccgccgcgaa acaaacgtta ggtacattaa
    21841 cacacattaa taatgcacaa cgtacagcgt tagataatga aattacacaa gcaacaaatg
    21901 ttgaaggtgt taatacagtt aaagccaaag cacaacaatt agatgttgct atgggtcaat
    21961 tagaaacatc aattcgtgat aaagacacga cgttacaaag tcaaaattat caagatgctg
    22021 atgatgctaa acgaactgct tattctcaag cagtaaatgc agcagcaact attttaaata
    22081 aaacagctgg aggaaataca cctaaagcag atgtcgaaag agcaatgcaa gctgttacac
    22141 aagccaatac tgcattaaac ggtattcaaa acttagaacg tgcgaaacag gctgcgaaca
    22201 cagcgattac aaatgcttcg gacttaaata caaaacaaaa agaagcatta aaagcacaag
    22261 taacaagtgc aggacgcgta tctgcagcaa atggtgttga acatactgcg actgaattaa
    22321 atactgcgat gacagcttta aaacgtgcca ttgctgataa agctgacaca aaagctagtg
    22381 gtaactatgt caatgctgat gcgaataaac gccaagcata tgatgaaaaa gtgacagctg
    22441 cagaaaatat cgttagtggt acaccaacac caacgttaac accatcagat gttacaaacg
    22501 cagcaacgca agtaacgaat gcgaagacac agttaaacgg taatcataat ttagaagtag
    22561 cgaaacaaaa tgctaacaca gcaattgatg gtttaacttc tttaaatggt ccgcaaaaag
    22621 caaaacttaa agaacaagtg ggtcaagcga cgacgttgcc aaatgttcaa actgttcgtg
    22681 ataatgcaca aacattaaac actgcaatga aaggtctacg agatagcatt gcgaatgaag
    22741 caacgattaa agcaggtcaa aactacacag atgcaagtca aaacaaacaa aatgactaca
    22801 acaatgcagt cactgcagca aaagcaatca ttggtcaaac aactagtcca acaatgaatg
    22861 cgcaagaaat caatcaagcg aaagaccaag tgacagctaa acaacaagcg ttaaatggtc
    22921 aagaaaactt aagaactgcg caaacaaatg cgaagcaaca tttgaatggc ttaagtgact
    22981 taactaatgc acaaaaagat gcagcgaaac gtcaaatcga gggtgcaacg catgttaatg
    23041 aagtaacaca agcacaaaat aatgcggatg cattaaatac agctatgacg aacttgaaaa
    23101 atggtattca agatcaaaat acgattaagc aaggtgttaa cttcactgat gcagatgaag
    23161 cgaaacgtaa tgcatataca aatgcagtga cgaaagctga acaaatttta aataaagcac
    23221 aaggtccaaa tactgcaaaa gacggtgtcg aaactgcgtt acaaaatgta caacgtgcta
    23281 aaaacgaatt gaacggtaat caaaatgttg cgaacgctaa gacaactgcg aaaaatgcat
    23341 tgaataacct aacatcaatt aacaatgcac aaaaagaagc attgaaaacg caaattgaag
    23401 gtgcgacaac agttgcaggt gtaaatcaag tgtctacaac agcatctgaa ttaaatactg
    23461 caatgagcaa cttacaacgt ggtattaacg atgaagcagc tacaaaagca gctcagaaat
    23521 atactgatgc agatagagaa aaacaaacag catacaatga tgctgtaaca gcagctaaaa
    23581 cgttattaga taaaacagct ggttcaaatg acaataaagc agccgttgaa caagcattac
    23641 aacgtgtgaa tactgctaaa acagcattaa atggtgacgc gcgattaaat gaagcgaaga
    23701 acacagctaa acaacaatta gcgacaatgt cacatttaac taatgctcaa aaagcaaact
    23761 taacagaaca aattgaacgc ggtacaactg ttgctggtgt tcaaggcatc caagcaaatg
    23821 ctggtacttt aaatcaagca atgaatcaat taagacaaag tattgcttct aaagatgcga
    23881 ctaaatcaag cgaagattat caagacgcga atgcagattt acaaactgca tacaatgatg
    23941 cggtaactaa tgctgagggt attattagtg caacgaataa ccctgaaatg aatccagata
    24001 cgattaatca aaaagcgagc caagtgaaca gtgcgaagtc tgcattgaac ggtgatgaaa
    24061 aattagcagc agcaaaacaa aatgcgaaat cagacatcgg tcgtttaaca gacttgaaca
    24121 atgcacaacg aactgcggca aatgcagaag tggatcatgc accaaacctt gcagctgtta
    24181 caacggctaa aaataaagca acatcgttaa atacagcgat gggtaatttg aaacatgcac
    24241 ttgctgaaaa ggataatacg aaacgtagtg tcaattacac agatgcggat caaccaaaac
    24301 aacaagcgta tgatactgca gttacacaag cagaaggaat tactaatgct aatggcagca
    24361 acgcgaatga aacacaagtt caagcagcgc ttaaccaatt gaatcaagct aaaaataact
    24421 tgaatggtga taataaagta gctcaagcta aagaagcagc aaaacgagca ttagcttcgt
    24481 acagtaactt gaataatgcg caatcaactg cagcaactag tcaaattgac aatgcaacga
    24541 cagtagcagg cgtaactgct gcacaaaata ctgctaatga gttaaataca gcaatgggtc
    24601 aacttcaaaa tggtattaat gaccaaaaca ctgttaaaca acaagtgaac tttacagatg
    24661 ctgaccaagg taagaaagat gcttacacaa atgctgttac gaatgctcaa ggtattttag
    24721 ataaagcaca cggtcaaaat atgacgaaag cacaagttga agctgcatta aatcaagtaa
    24781 cgactgctaa gaatgcttta aacggtgacg caaatgtaag acaagcaaaa tcagatgcga
    24841 aagcaaactt aggtacatta acacacttaa ataatgcaca aaaacaagat ttaacatcac
    24901 aaatcgaagg cgcaacaaca gtcaacggtg taaatggtgt taaaacgaaa gcacaagact
    24961 tagatggtgc aatgcaacga ttacaatcag ctatagcaaa taaagatcaa actaaagcga
    25021 gcgaaaatta catcgacgca gatccaacta agaaaacagc atttgataat gctatcacac
    25081 aagctgaatc ttacttaaat aaagatcacg gtgcgaataa agataagcaa gctgttgaac
    25141 aagcaattca aagtgtaacg tctactgaaa atgctttgaa cggtgacgcg aacttacaac
    25201 gcgctaaaac tgaagctata caagctatcg ataacttgac acatttgaat acaccacaaa
    25261 aaacagcatt aaaacaacaa gtgaacgctg cgcaacgtgt atcaggtgta actgatctga
    25321 aaaatagtgc tacatcactt aataatgcga tggatcaatt aaaacaagca attgctgatc
    25381 atgacacaat tgtagctagt ggtaattaca ctaacgcgag tcctgataag caaggtgctt
    25441 atactgatgc atataatgct gcgaaaaaca ttgtaaatgg ttcgcctaat gtgattacaa
    25501 atgcagcaga tgttacagca gcaacacaac gtgttaataa tgctgaaaca ggtttaaacg
    25561 gtgatacaaa cttagcaact gcgaagcaac aagctaaaga tgcattacgt caaatgacac
    25621 atttatctga tgcacaaaaa caaagtatta ctggtcaaat tgatagcgcg acacaagtaa
    25681 ctggtgtaca aagtgtgaaa gacaatgcaa caaatcttga caatgcaatg aatcaacttc
    25741 gaaatagtat tgcgaataaa gatgaagtaa aagcgagtca accatatgtt gatgcagata
    25801 cagataaaca aaatgcatac aatacagcag ttacaagtgc tgaaaatatc attaatgcaa
    25861 cgagtcagcc aacacttgat ccatctgcag taacacaagc agctaatcaa gtgaacacta
    25921 acaaaacggc gcttaatggt gcgcaaaact tagcaaataa aaagcaagaa acaactgcta
    25981 acatcaaccg attaagtcat ttaaacaatg ctcaaaagca agatttaaat acacaagtga
    26041 caaatgcacc aaatattagc acagtaaatc aagtgaaaac taaagctgaa caattagatc
    26101 aagcaatgga acgtttaatc aacggaatcc aagacaaaga tcaagtgaaa caaagtgtta
    26161 actttacaga tgcagatcca gaaaaacaaa cagcatacaa caatgcggta actgctgctg
    26221 aaaatattat taatcaagca aatggtacaa atgcgaacca atcacaagtt gaagcagcac
    26281 tttcaactgt aacaactact aaacaagcgt tgaatggtga tagaaaagta acagatgcta
    26341 aaaacaatgc aaaccaaaca ttatctacgt tagataactt aaacaatgca caaaaaggtg
    26401 ctgttactgg aaacatcaat caagcgcaca ctgtagctga agtaacgcaa gccattcaaa
    26461 ccgctcagga actgaataca gcgatgggta acttgaaaaa tagcttgaat gataaagaca
    26521 ctacacttgg cagtcaaaac tttgcagatg cagatccaga gaagaaaaat gcatacaatg
    26581 aagcggttcg taatgctgaa aatattttaa ataaatctac aggtacgaac gtgcctaaag
    26641 atcaagttga agcagctatg aatcaagtga atactacaaa agcagcgctt aatggtactc
    26701 aaaaccttga aaaagcgaaa caacacgcaa atacagcaat tgacggttta agccatttaa
    26761 caaatgcaca aaaagaggca ttaaaacaat tggtacaaca atcgactact gttgcagaag
    26821 cacaaggtaa tgaacaaaaa gcaaacaatg ttgatgcagc aatggacaaa ttacgtcaaa
    26881 gtattgcaga taatgcgaca acaaaacaaa accaaaatta tactgatgca agtccgaata
    26941 aaaaggatgc gtacaataat gctgtcacaa ctgcacaagg tattattgat caaactacaa
    27001 atccaacttt agatccgact gttatcaatc aagctgctgg tcaagtaagc acaactaaaa
    27061 atgcattaaa tggtaatgaa aacctagagg cagcgaaaca acaagcgaca caatcattag
    27121 gttcattaga caacttaaat aatgcgcaaa aacaagctgt tactaatcaa attaatggcg
    27181 cgcatactgt tgatgaagca aatcaaatta aacaaaatgc gcaaaactta aatacagcga
    27241 tgggtaactt gaaacaagcg atagctgaca aagatgctac gaaagcaaca gttaacttca
    27301 ctgatgcaga tcaagccaaa caacaagcat ataacactgc tgttacaaat gctgaaaaca
    27361 ttatttcaaa agctaatggt ggcaatgcaa cacaaactga agttgaacaa gcaatccaac
    27421 aagttaatgc aacaaaacaa gcattaaatg gtaatgccaa cgttcaacat gcaaaagacg
    27481 aagcaacagc attaattaat agctctaatg accttaacca agcacaaaaa gacgcattaa
    27541 aacaacaagt tcaaaatgca actactgtag ctggtgtaaa caatgttaaa caaacagcac
    27601 aagagttaaa caatgcgatg acacaattaa aacaaggcat tgcagataaa gaacaaacaa
    27661 aagctgatgg taactttgtc aatgcagatc ctgacaagca aaatgcatat aatcaagcag
    27721 tagcaaaagc tgaagcatta attagtggta cgcctgatgt agtcgttaca cctagcgaaa
    27781 ttactgcagc tttaaataaa gttacgcaag ctaaaaatga tttgaatggt aatacgaact
    27841 tagcaacggc gaaacaaatt gttcaacaag ctattgatca attgccaaac ttgaatcaag
    27901 cgcaacgtga tgaatacaac aaacaaatta cgcaagcaac tcttgtacca aacgtcaatg
    27961 ctattcaaca agcggcgaca acgcttaatg acgtgatgac acaattgaaa caaggtattg
    28021 cgaataaagc acaaattaaa ggtagcgaga actatcacga tgctgatact gacaagcaaa
    28081 cagcatatga taatgcagta acaaaagcag aagaattgtt aaaacaaaca acaaatccaa
    28141 caatggatcc aaatacaatt caacaagcat taactaaagt gaatgacaca aatcaagcac
    28201 ttaacggtaa tcaaaaatta gctgatgcca aacaagatac taagacaaca cttggtacac
    28261 tagatcattt aaatgatgct caaaaacaag cgctaacaac tcaagttgaa caagcaccag
    28321 atattgcaac agttaataat gttaagcaaa atgctcaaaa tctgaataat gctatgacta
    28381 acttaaacaa tgcattacaa gataaaactg agacattaaa tagcattaac tttactgatg
    28441 cagatcaagc taagaaagat gcttatacta atgcggtagc aaatgcagaa ggtattttat
    28501 ctaaagcaaa tggcagcaat gcaagtcaaa ctgaagtgga acaagcgatg caacgtgtga
    28561 acgcagcgaa acaagcattg aatggtaatg acaatgtaca acgtgcaaaa gatgcagcga
    28621 aacaagtgat tacaaatgca aatgatttaa atcaagcgca aaaagatgca ttaaaacaac
    28681 aagttgatgc tgcgcaaact gttgcaaatg taaacacgat taagcaaaca gcacaagatt
    28741 taaatcaagc aatgacacaa ttgaaacaag gtattgcaga taaagaccaa actaaagcaa
    28801 atggtaactt tgtcaatgct gatactgata agcaaaatgc atataacaat gcggtagcgc
    28861 atgctgaaca aatcattagt ggtacaccaa atgcaaacgt ggatccacaa caagtggctc
    28921 aagcgttaca acaagtgaat caagctaagg gtgatttaaa cggtaaccat aacttacaag
    28981 ttgctaaaga caatgcaaat acagccattg atcagttacc aaacttaaat caaccacaaa
    29041 aaacagcatt aaaagaccaa gtgtcgcatg cagaacttgt tacaggtgtt aatgctatta
    29101 agcaaaatgc tgatgcgtta aataatgcaa tgggtacgtt gaaacaacaa attcaagcga
    29161 atagtcaagt accacaatca gttgacttta cacaagcgga tcaagacaaa caacaagctt
    29221 ataacaatgc agctaaccaa gcgcaacaaa tcgcaaatgg cacaccaaca cctgtattgg
    29281 cgcctgatac agtaacacaa gcagttacaa ctatgaatca agcgaaagat gcattaaacg
    29341 gtgatgaaaa attagcgcaa gcgaaacaag atgctttagc aaatcttgat acgttacgtg
    29401 acttaaatca accacaacgt gatgcattac gaaaccaaat caatcaagca caagctttag
    29461 ctacagttga acaaactaaa caaaatgcac aaaatgtgaa tacagcaatg ggtaacttga
    29521 aacaaggtat tgcaaataaa gatactgtga aagcaagtga gaactaccac gatgctgatg
    29581 tcgataagca aacagcatat acaaatgcag tgtctcaagc ggaaggtatt atcaatcaaa
    29641 cgacaaatcc aacgcttaac ccagatgaca ttactcgtgc attaactcaa gtgactgatg
    29701 ctaaaaatag cttaaacggt gaagctaaat tagccactga aaagcaaaat gctaaagatg
    29761 ccgtaagtgg aatgacgcat ttaaacgatg ctcaaaaaca agcattaaaa ggtcaaatcg
    29821 atcaatcgcc tgaaattgct acagtgaacc aagttaaaca aacagcaacg agcctagatc
    29881 aagcaatgga tcaattatca caagctatta atgataaaga tcaaatatta gcggacggta
    29941 attacttaaa tgcagatcct gacaaacaaa atgcgtataa acaggcagta gcaaaagctg
    30001 aagcattatt gaataaacaa agtggtacta atgaagtaca agcacaagtt gaaagcatca
    30061 ctaatgaagt gaacgcagcg aaacaagcat taaatggtaa tgacaatttg gcaaatgcaa
    30121 aacaacaagc aaaacaacaa ttggcgaact taacacactt aaatgatgca caaaaacaat
    30181 catttgaaag tcaaattaca caagcgccac ttgttacaga tgtcactacg attaatcaaa
    30241 aagcacaaac gttagatcat gcgatggaat tattaagaaa tagtgttgcg gataatcaaa
    30301 cgacattagc gtctgaagat tatcatgatg caactgcgca aagacaaaat gactataaca
    30361 aagctgtaac agctgctaat aatatcatta atcaaactac atcgcctacg atgaatccag
    30421 atgatgttaa tggtgcaacg acacaagtga ataatacgaa agttgcatta gatggtgatg
    30481 aaaaccttgc agcagctaag caagaagcga acaacagact tgatcaatta gatcatttga
    30541 ataatgcgca aaagcaacag ttacaatcac aaattacgca atcatctgat attgctgcag
    30601 ttaatggtca caaacaaaca gcagaatctt taaatactgc gatgggtaac ttaattaatg
    30661 cgattgcaga tcatcaagcc gttgaacaac gtggtaactt catcaatgct gatactgata
    30721 aacaaactgc ttataataca gcggtaaatg aagcagcagc aatgattaac aaacaaactg
    30781 gtcaaaatgc gaaccaaaca gaagtagaac aagctattac taaagttcaa acaacacttc
    30841 aagcgttaaa tggagatcat aatttacaag ttgctaaaac aaatgcgacg caagcaattg
    30901 atgctttaac aagcttaaat gatcctcaaa aaacagcatt aaaagaccaa gttacagctg
    30961 caactttagt aactgcagtt catcaaattg aacaaaatgc gaatacgctt aaccaagcaa
    31021 tgcatggttt aagacagagc attcaagata acgcagcaac taaagcaaat agcaaatata
    31081 tcaacgaaga tcaaccagag caacaaaact atgatcaagc tgttcaagcc gcaaataata
    31141 ttatcaatga acaaactgca acattagata ataatgcgat taatcaagca gcggcaactg
    31201 tgaatacaac gaaagcagca ttacatggtg atgtgaaatt acaaaatgat aaagatcatg
    31261 ctaaacaaac ggttagtcaa ttagcacatc taaacaatgc acaaaaacat atggaagata
    31321 cgttaattga tagcgaaaca actagaacag cagttaagca agatttgact gaagcacaag
    31381 cattagatca acttatggat gcattacaac aaagtattgc tgacaaagat gcaacacgtg
    31441 cgagcagtgc atatgtcaat gcagaaccga ataaaaaaca agcctatgat gaagcagttc
    31501 aaaatgctga gtctatcatt gcaggattaa ataatccaac tatcaataaa ggtaatgtat
    31561 caagtgcgac tcaagcagta atatcatcta aaaatgcatt agatggtgtt gaacgattag
    31621 ctcaagataa gcaaactgct ggaaattctc taaatcattt agatcaatta acaccagctc
    31681 aacaacaagc gctagaaaat caaattaata atgcaacaac tcgtgataaa gtggctgaaa
    31741 tcattgcaca agcgcaagca ttaaatgaag cgatgaaagc attaaaagaa agtattaagg
    31801 atcaaccaca aactgaagct agtagtaaat ttattaacga ggatcaagcg caaaaagatg
    31861 cttatacgca agcagtacaa cacgcgaaag atttgattaa caaaacaact gatcctacat
    31921 tagctaaatc aatcattgat caagcgacac aggcagtgac tgatgctaaa aacaatttac
    31981 atggtgatca aaaactagct caagataagc aacgtgcaac agaaacgtta aataacttgt
    32041 ctaacttgaa tacaccacaa cgtcaagcac ttgaaaatca aatcaataat gcggcaactc
    32101 gtggcgaagt agcacaaaaa ttaactgaag cacaagcact taaccaagca atggaagctt
    32161 tacgtaatag cattcaagat caacagcaaa cggaagcggg tagcaagttt atcaatgaag
    32221 ataaaccaca aaaagatgct taccaagcag cagttcaaaa tgcaaaaaat ttaattaatc
    32281 aaactaacaa tccaacgctt gataaagcac aagttgaaca attgacacaa gctgttaacc
    32341 aagctaaaga taacctacac ggtgatcaaa aacttgcaga cgataaacaa catgcggtta
    32401 ctgatttaaa tcaattaaat ggtttgaata atccgcaacg tcaagcactt gaaagccaaa
    32461 taaacaacgc agcaactcgt gatgaagtag cgcaaaaatt agctgaagca aaagcgcttg
    32521 atcaagcaat gcaagcatta cgaaatagta ttcaagatca acaacaaaca gaatctggta
    32581 gcaagtttat taatgaagat aaaccgcaaa aagatgctta ccaagcagca gttcaaaatg
    32641 caaaagattt aattaaccaa acaggtaatc caacactcga caaatcacaa gtagaacaat
    32701 tgacacaagc agtaacaact gcaaaagata acctacatgg tgatcaaaaa cttgctcgtg
    32761 atcaacaaca agcagtaacg actgttaatg cattgccaaa cttaaatcat gcacaacaac
    32821 aagcattaac tgatgctata aatgcagcgc ctacaagaac agaggttgca caacatgttc
    32881 aaactgctac tgaacttgat cacgcaatgg aaacattgaa aaataaagtt gatcaagtga
    32941 acacagataa ggctcaacca aattacactg aagcatcaac ggataaaaaa gaagcagttg
    33001 atcaagcgtt acaagctgct caaagcatta cagatccaac taatggttca aatgcaaata
    33061 aagatgctgt agaacaagca ttaacgaagc tgcaagaaaa agaaaatgag ttgaatggta
    33121 atgagagagt cgctgaagct aaaacacaag cgaaacaaac tattgaccaa ttaacacatt
    33181 taaatgctga tcaaattgca actgctaaac aaaacattga tcaagcgacg aaacttcaac
    33241 caattgctga attagtagat caagcaacgc aattgaatca atctatggat caattacaac
    33301 aagcagttaa tgaacatgct aacgttgagc aaactgtaga ttacacacaa gcagattcag
    33361 ataagcaaaa tgcttataaa caagctattg ctgatgctga aaatgtattg aaacaaaatg
    33421 cgaataagca acaagtggat caagcacttc aaaatatttt aaatgcaaaa caagcattaa
    33481 atggtgatga acgtgtagca cttgctaaaa caaatggtaa acatgacatc gaccaattga
    33541 atgcattaaa caatgctcaa caagatggat ttaaaggtcg catcgatcaa tcaaacgatt
    33601 taaatcaaat ccaacaaatt gtagatgagg ctaaggcact taatcgtgca atggatcaat
    33661 tgtcacaaga aatcactggc aatgaaggac gcacgaaagg tagcacgaac tatgtcaatg
    33721 cagatacaca agtcaaacaa gtatatgacg aagcggttga taaagcgaaa caagcacttg
    33781 ataaatcgac tggtcaaaac ttaactgcag aacaagttat caaattaaat gatgcaatca
    33841 ctgcagctaa gaaagcatta aatggtgaag aaagacttaa taatcgtaaa gctgaagcat
    33901 tacaaagatt ggatcaatta acacatctaa acaatgctca aagacaatta gcaatccaac
    33961 aaattaataa tgctgaaacg ctaaataaag catctcgagc aattaataga gcaactaaat
    34021 tagataatgc aatgggtgca gtacaacaat atattgacga acagcacctt ggtgttatca
    34081 gcagcacaaa ttacatcaat gcagatgaca atttgaaagc aaattatgat aatgcaattg
    34141 cgaatgcagc acatgagtta gataaagtgc aaggtaatgc aattgcaaaa gctgaagcag
    34201 agcaattgaa acaaaatatt atcgatgctc aaaatgcatt aaatggagac caaaaccttg
    34261 caaatgccaa agataaagca aatgcgtttg ttaattcgtt aaatggatta aatcaacagc
    34321 aacaagatct tgcacataaa gcaattaaca atgccgatac tgtatcagat gtaacagata
    34381 ttgttaataa tcaaattgac ttaaatgatg caatggaaac attgaaacat ttagttgaca
    34441 atgaaattcc aaatgcagag caaactgtca attaccaaaa cgctgacgat aatgctaaaa
    34501 caaacttcga tgatgccaaa cgtctagcaa atacattgct aaatagtgat aacacaaatg
    34561 tgaatgatat taacggcgca attcaaacag tcaatgatgc aatccataat cttaatggtg
    34621 atcaacgact acaagatgct aaagacaagg caattcaatc tattaatcaa gctttagcta
    34681 ataagctaaa agaaatcgaa gcttcaaatg cgacggatca agacaagcat attgcgaaaa
    34741 ataaagcaga agaattggca aacagcatca tcaacaacat taataaagca acaagtaatc
    34801 aggctgtatc tcaagttcaa acagcaggca agcacgcgat tgaacaagtg catgccaatg
    34861 aaataccaaa agctaaaatt gatgccaata aagacgttga taagcaagtt caatcattaa
    34921 ttgacgaaat tgatcgaaat ccaaatctaa cagataagga aaaacaagca cttaaagatc
    34981 gtattaatca aatacttcaa caaggtcata acggcattaa caatgcgatg acaaaagaag
    35041 aaattgaaca agccaaagca caacttgcgc aagcattaca agacatcaaa gatttagtga
    35101 aagctaaaga agatgcgaaa caagatgttg ataaacaagt tcaagcatta attgacgaaa
    35161 tcgatcaaaa tccaaatcta acagataagg aaaaacaagc acttaaagat cgaattaatc
    35221 aaatacttca acaaggtcat aacggcatta acaatgcgat gacaaaagaa gaaattgaac
    35281 aagccaaagc acaacttgca caagcattac aagacatcaa agatttagtg aaagctaaag
    35341 aaaatgcgaa acaagatatt gataaacgtg tacaagcttt aattgacgaa atcgatcaaa
    35401 atccaaatct aacagataag gaaaaacaag cacttaaaga tcgaattaat caaatacttc
    35461 aacaaggtca t
//
LOCUS       DAHHYJ010000010        35312 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000010 DAHHYJ010000000
VERSION     DAHHYJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 35312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.010"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(123..1292)
                     /locus_tag="NBK22_000376"
     CDS             complement(123..1292)
                     /locus_tag="NBK22_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD2413592.1"
                     /translation="MIKNTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVTKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFKKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGHASQRICQTIFK"
     gene            complement(1720..3414)
                     /locus_tag="NBK22_000377"
     CDS             complement(1720..3414)
                     /locus_tag="NBK22_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD2413593.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     EGLNEEQIEEYNELTTQNGKANYVLANEAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(3436..4461)
                     /locus_tag="NBK22_000378"
     CDS             complement(3436..4461)
                     /locus_tag="NBK22_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD2413594.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRKKLPMSLVHEAVGEVVFDSKGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKTYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYRKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(4454..5170)
                     /locus_tag="NBK22_000379"
     CDS             complement(4454..5170)
                     /locus_tag="NBK22_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCD2413595.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNVNGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHAVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
     gene            complement(5698..6741)
                     /locus_tag="NBK22_000380"
     CDS             complement(5698..6741)
                     /locus_tag="NBK22_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD2413596.1"
                     /translation="MKALKLYGVEDLRYEDNEKPVIESANDVIIKVRATGICGSDTSR
                     YKKMGPYIKGMPFGHEFSGVVDAIGSDVTHVNVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEENLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSNFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
     gene            complement(6765..6911)
                     /locus_tag="NBK22_000381"
     CDS             complement(6765..6911)
                     /locus_tag="NBK22_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413597.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            complement(6913..7968)
                     /locus_tag="NBK22_000382"
     CDS             complement(6913..7968)
                     /locus_tag="NBK22_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCD2413598.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYVCNECEYCKSKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLQLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            complement(7986..9245)
                     /locus_tag="NBK22_000383"
     CDS             complement(7986..9245)
                     /locus_tag="NBK22_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD2413599.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYSVSGSIWQALLAAVIFQIICL
                     KVADWTAPMMSEFFDLPGVSIATGSTISYAPGIYLVKLLQKVPGLNKLDADPETIQKR
                     FGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLMPVS
                     ESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVLPFG
                     DLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQMPK
                     GSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            complement(9472..9750)
                     /locus_tag="NBK22_000384"
     CDS             complement(9472..9750)
                     /locus_tag="NBK22_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD2413600.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEMAKEHNIKVYIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            complement(9773..10240)
                     /locus_tag="NBK22_000385"
     CDS             complement(9773..10240)
                     /locus_tag="NBK22_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2413601.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMNKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            complement(10225..12321)
                     /locus_tag="NBK22_000386"
     CDS             complement(10225..12321)
                     /locus_tag="NBK22_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCD2413602.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLEDHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDNISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKDVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRMHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARGNGWDSN"
     gene            complement(12452..12549)
                     /locus_tag="NBK22_000387"
                     /pseudo
     CDS             complement(12452..12549)
                     /locus_tag="NBK22_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12659..13594)
                     /locus_tag="NBK22_000388"
     CDS             complement(12659..13594)
                     /locus_tag="NBK22_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD2413603.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKSAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDVIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADNHVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSSLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDYKLRTYE"
     gene            13956..15485
                     /locus_tag="NBK22_000389"
     CDS             13956..15485
                     /locus_tag="NBK22_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2413604.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMSDIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGKDNIEI
                     VDCCATRLRVTLHQNDKVDKALLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            complement(15805..16758)
                     /locus_tag="NBK22_000390"
     CDS             complement(15805..16758)
                     /locus_tag="NBK22_000390"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD2413605.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAEAEELK"
     gene            complement(17069..17176)
                     /locus_tag="NBK22_000391"
     CDS             complement(17069..17176)
                     /locus_tag="NBK22_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413606.1"
                     /translation="MSVCTPTIARIYLTNSKNIVVFLEKSKHDNKNVKV"
     gene            17331..18476
                     /locus_tag="NBK22_000392"
     CDS             17331..18476
                     /locus_tag="NBK22_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCD2413607.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLSYRAKRHYSIVSGEKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQV
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDCVHYETFIPRLSVAV"
     gene            18502..18675
                     /locus_tag="NBK22_000393"
     CDS             18502..18675
                     /locus_tag="NBK22_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413608.1"
                     /translation="MKLNQRYVKVFALYFASIVTANIVVKNNNLIKTLIQTLAGYTVF
                     AVGLKYLTKRKNK"
     gene            18831..19187
                     /locus_tag="NBK22_000394"
     CDS             18831..19187
                     /locus_tag="NBK22_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCD2413609.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDAQKDAFEKLKDIVNQHNHVLLLYTAK
                     DTKHNQAVVLQQLLNT"
     gene            19385..20860
                     /locus_tag="NBK22_000395"
     CDS             19385..20860
                     /locus_tag="NBK22_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2413610.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(21174..22316)
                     /locus_tag="NBK22_000396"
     CDS             complement(21174..22316)
                     /locus_tag="NBK22_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCD2413611.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMLSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVVLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            22757..24334
                     /locus_tag="NBK22_000397"
     CDS             22757..24334
                     /locus_tag="NBK22_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCD2413612.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEIYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            24360..25865
                     /locus_tag="NBK22_000398"
     CDS             24360..25865
                     /locus_tag="NBK22_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCD2413613.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFRAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDNDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILNQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            25977..27188
                     /locus_tag="NBK22_000399"
     CDS             25977..27188
                     /locus_tag="NBK22_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCD2413614.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDYFKDLRELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            27374..29635
                     /locus_tag="NBK22_000400"
     CDS             27374..29635
                     /locus_tag="NBK22_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HCD2413615.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQAFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKISIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLVHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     TRMEDELGDLPQWISDLDGGFYKQDETIEYATPVSHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKALIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            29665..30849
                     /locus_tag="NBK22_000401"
     CDS             29665..30849
                     /locus_tag="NBK22_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCD2413616.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALLTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIRVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            31163..31270
                     /locus_tag="NBK22_000402"
     CDS             31163..31270
                     /locus_tag="NBK22_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413617.1"
                     /translation="MCVRTRLVSSSSVRLSKAIIIAVIVVCHIDVRGLF"
     gene            complement(31452..33434)
                     /gene="coa"
                     /locus_tag="NBK22_000403"
     CDS             complement(31452..33434)
                     /gene="coa"
                     /locus_tag="NBK22_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCD2413618.1"
                     /translation="MKKQIISLGALAVASSLFTWDNKADAIVTKDYSGKSQVNAGSKN
                     GKQIADGYYWGIIENLENQFYNIFHLLDQHKYAEKEYKDAVDKLKTRVLEEGQYLLER
                     KKEKYEIYKELYKKYKKENPNTQVKMKAFDKYDLGDLTMEEYNDLSKLLTKALDNFKL
                     EVKKIESENPDLKPYSESEERTAYGKIDSLVDQAYSVYFAYVTDAQHKTEALNLRAKI
                     DLILGDEKDPIRVTNQRTEKEMIKDLESIIDDFFIETKLNRPKHITRYDGTKHDYHKH
                     KDGFDALVKETREAVAKADESWKNKTVKKYEETVTKSPVVKEEKKVEEPQSPKFDNQQ
                     EVKITVDKAEETTQPVAQPLVKIPQGTITGEIVKGPEYPTMENKTLQGEIVQGPDFLT
                     MEQNRPSLSDNYTQPTTPNPILEGLEGSSSKLEIKPQGTESTLKGIQGESSDIEVKPQ
                     ATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYN
                     VTTNQDGTVSYGARPTQNKPSETNAYNVTTHANGQVSYGARPTQNKPSETNAYNVTTH
                     ANGQVSYGARPTYKKPSKTNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHANGQ
                     VSYGARPTQNKPSKTNAYNVTTHADGTATYGPRVTK"
     gene            33624..33968
                     /locus_tag="NBK22_000404"
     CDS             33624..33968
                     /locus_tag="NBK22_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCD2413619.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLHELIANTDLNKLSYLNLDAYQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQNN"
     gene            complement(34132..>35312)
                     /locus_tag="NBK22_000405"
     CDS             complement(34132..>35312)
                     /locus_tag="NBK22_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD2413620.1"
                     /translation="LNRQSLFKNMRLSWQITKRNKFRLVIEIVILELIIGAILTLIIS
                     GATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFTKLSLISVLVLHLKQENVLDQP
                     GLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYNNTINTNISIIGHRGFEDKGVE
                     NSIPSLKAAAKANVEYVELDTIMTKDKQFVVSHDNNLKRLTGVNKNISESNFKDVVGL
                     KMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGKEPADYTQRVIDILKKHGVEHQ
                     YRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKETSLDFFVIEDFSYSPRLVNQAH
                     LENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALADQIKEEKKDETYFDRSIRILFE
                     "
BASE COUNT        11223 a         6453 c         5763 g        11873 t
ORIGIN      
        1 aaacctatat tacattatat cagacgtttt catacacaaa taataacata caagcaaaca
       61 tttcgtttat tatttatatc acttaactaa ttaatttata attttttatt gtttttaagt
      121 tatcacttaa aaatcgtttg gcaaattcgt tgtgacgcgt gtccatcttc taatgaacag
      181 aatttttgat aaaataccgt tcgtgcttca atatactcat ttgcagtctc atcgatttgt
      241 tttaatgcat caatgagtgc tgtttgattt tcaacaattg gacctggcaa ctctttttta
      301 taatccatgt aaaaacctct aagctcatcg ccatatttat ctaagtcata cgcatagaaa
      361 atttgcggac gctttaatac accgaagtcg aacatgacag atgagtagtc ggtaactaac
      421 gcatcgctga ttaagtataa atccgaaata tcttcataat ctgaaacgtc tttcacgaaa
      481 tcatcatgtt catcaatacg tgtcacaact aaataatgca tgcgtaataa aataacataa
      541 tcatcatcca gcgcttgacg caaagcttct atatcaaagt taacattaaa ttgatatgaa
      601 ccttctcgaa tcgcttcatc gtcacgccaa gttggcgcgt acataatcac ttttttatct
      661 aatggaatat ttaatcttgt cttaatacca ttaatatatt cagtatcatt gcgtttatgt
      721 gataatttat catttcttgg ataacctgtt tccaaaatct tatctcgact aacatgaaat
      781 gcatgttgaa atatcgatgt cgaatatgga ttaggtgaca ctagataatc ccaccgttgg
      841 ctttcttttt taaagccatc ttggtaattt tgagtatttg ttcctagcat tttaacgtta
      901 ctaatatcca aaccaatctt ttttaatggc gtgccatgcc atgtttgtaa gtacgtcgtt
      961 cgcggtgatt tatataacca atctggtgta cgtgtgttaa tcatccacgc tttcgctctt
     1021 ggcatcgcta aaaaccattt cattgaaaac tttgtaacat atggtacatt gtgctgttgg
     1081 aatatgtgtt catatccttt tttcacaccc catattaatt gggcatcgct atgttcagtt
     1141 aagtattcat ataatgcttt ggggttgtcg ctgtattgtt taccatgaaa gctttcaaaa
     1201 taaattagat tcttgtttgg caattttgat agtaatttaa aagtcgtata tatactatgt
     1261 tctatcaatt ttttaattgt atttttaatc atgtcgtacc tccgacgtgt ttttgtaatt
     1321 atattaatat gtatgagcaa gctcattgta accatgccta ttatagcatt tcatcataaa
     1381 atacatttaa ccattacact tgtcgttaat tatcatacga aatacatgat taatgtacca
     1441 ctttaacata acaaaaaatc gttatccatt cataacgtat gtgtttacac atttatgaat
     1501 tagataacga ttggatcgat tattttattt tacaaaatga caattcagtt ggaaggtgat
     1561 tgcttttgat tgaatcgcct tatgcatgaa aaatcaaaag gttattctca ttgtatagtc
     1621 ctgcttctca tcatgacatg ttgctaactt cattgtcaga acccttcttg aaaactatgc
     1681 cttatgactc atttgcatgg caagtaatat atgccaacat tagcgtctaa acaaatcttt
     1741 gactaaacgt tcacttgagc gaccatcttg atatttaaaa tgtttatcta agaatggtac
     1801 aactttttca acctcataat cttcattgtc caaagcatcc attaatgcat caaaggattg
     1861 tacaatttta cctggaacaa atgattcata tggttcatag aaatcacgcg tcgtaatgta
     1921 atcttctaag tcaaatgcat agaaaatcat cggcttttta aatactgcat attcatatat
     1981 taaagatgaa taatcactaa tcaacaagtc tgtaacaaag agaatatcgt taacttcacg
     2041 atgatctgac acatcgataa agtattgtct atgttcacgt gaaatattaa gtctattttt
     2101 tacgaacgga tgcattttga ataacacaac tgcattatgc ttctcgcagt atcttgctaa
     2161 acgttcaaaa tcaattttaa aaaatggata atgtgccgta ccgtgaccat tacctctaaa
     2221 tgtcggtgcg aatagaataa ctttcttacc ttttataatt ggcaattcat cttccatctc
     2281 ttgtttaatt tgtgttgcat aagcttcatc aaatagtaca tcagtacgtg gtacacctgt
     2341 tggtacaaca ttttcttctc taattccaaa tgcttcagca taaaatggaa tatcggtttc
     2401 tgatgaaaca tatgctttcg tgtaactacg atggtttaat gaatcgataa acggaccacc
     2461 tttttttcct gtacgactaa agccaactgt tttaaaagca ccaacggcat gccacacttg
     2521 aataatttct tgtgatggtc taaagcgaac cgtataaatt aatggatgga aatcatcaac
     2581 aaaaatataa tctgccttac caagtaaata tggcaatcta aacttgtctc tccatttacg
     2641 tctatccgta atattcgcct taaataccgt tttaatatca taatcaaaat ctactttttg
     2701 gcgaagtaac tcatcatata catacttgaa attccctgat aaattcggac gcgaatctga
     2761 tgtgaataat attgttttgc ttcttttaat atgtagtaac tttgtaatat taaaaatagc
     2821 tttaaataag aaacttctac tttcaaatga agctttatgt ccttgtttat gaagccagtg
     2881 tgcgcttgtc gcaatgaccc ctgatttctc ttgaggtaag gtgatttcaa tatcaaatac
     2941 aaattcgtta acgtcacttg ctatttccgg agtaatggta tagaccgtat gttgtgatac
     3001 gccacctttg cggaaaactt tagcttcgtt ggctaacaca tagtttgctt ttccattttg
     3061 tgtagtcagc tcattgtatt cttcaatttg ctcttcattt aatccctcat aagcttgatc
     3121 aataatattt ggattaagtt gtccaatata ctctaactgg tcatgataaa caaataaata
     3181 tttatccatt ggtaaataat acccatcgtc taatattgcg acgttgaatc tacatataaa
     3241 tgtattaccg tctattttca tttcatttgc ttctaatgtt tttgtttcgg ttaaattcct
     3301 cagaacaaat tgtcctgatg ttaaatcgac accttcacta tgtccttcaa tatataactg
     3361 aacacgttcc cagtatatgt tatcaatatg tattgcttgt ttcgtttttg tcattgttat
     3421 tcccccatcg gtttattaaa tcgtccattt caatactgtt tttccccaag atgtcgataa
     3481 atccatttca aacgcttgga cgatatcttg catcgtacat acattaattt catgtccttt
     3541 taataatgct aacttttcaa caatgtctgg gtacttacga tataaatcaa caacttgctc
     3601 aaaatcttta gagccgcttc gactactacc aatcaacgtt aatccttttt caagtactaa
     3661 tcgtgtattc acttccacgg gtaattcact tacgcctaac aaagcaatac tgccttctgg
     3721 tgaaatatgt tcaactattt gttgaagtgc aacttgactt cctttacctc caacacattc
     3781 aaatgcatga tcaattttaa gatcatctgg tatttgattt actgtaaaga tgtcatctac
     3841 aaatgaaaaa tgacttaatt tatagtctgt cttaccaaat acataagttt tagcttctgg
     3901 gtacaactta cgtagcaaaa tagcagtaat ataacctaag ttaccatcac cccaaatacc
     3961 aaagctggtt ttcaaaggta tagatttacg ttcaaatcgt tgtatagcat gataacttac
     4021 tgacactaac tctgtgtatg aaatcgtact caaatcaatg tcattaggca gcggaacgat
     4081 acgatcatgt gccatcacaa cgtagtcttg cataaaacca tcataaccac tagatctaaa
     4141 ataactagag gctaagtaat tctccgcaat aatatgatgt tgctctgtag gtgtattcgg
     4201 taccattact actttcgtac ctttttcaaa taccccttta ctatcaaata caacttcacc
     4261 aacagcttca tgaactaatg acattggtaa ttttttgcgt agtacatttt catctcttcg
     4321 acctgtgtaa tacctttgat cagctgcaca aatagacaag tataaaggtc ttacgatgac
     4381 atgattacca taaatatcaa cattattata tgtgacgtcg aactgtctcg gtgcaacgag
     4441 ttgatatact tgattaatca tcggcaatat caccttgaat aatggcattt gctactttta
     4501 aatcatacgg tgttgtcact ttaatgttgt atagttctcc acgtaccaat ttaactgcat
     4561 gtccagattc gacaatgatt ttacatgcat ctgataagat ttctttttgt tcactactta
     4621 aggcgcgata actatcttgt aataatttaa tattaaatga ttgtggtgtt tggccttgat
     4681 acatttcatt ccttacaggg atactgtgta tgttctgttt atctttagac attacaatcg
     4741 tatcaattgc ttcaatgact gtatctactg caccatattt tgctgctact tcaatgttct
     4801 ctttaataat acgttgagtt aaaaatggtc ttacggcatc atgagttaca atcacatcat
     4861 cattattaat tccatttaca ttgcgaatat ggtcgataat gttcataatt gtttcatttc
     4921 gatccgtacc acctgcaact actttgacac gttgatctgt aatgttatat ttttttaaaa
     4981 tatcctgtgt atgggaaatc cactgtgctg gcgttgcgat aataatctca ttaaattcac
     5041 tcactaaaat gaacttctca attgtatgga ttaaaatcgg tttattatca atatctaaaa
     5101 attgttttgg taatggcacg ttccccattc tcgaaccaat acctcctgct aaaatacctg
     5161 cataaatcat gttgtcctcc attctgtcat tacatcattt ccatttatac attactgacc
     5221 tatgcccgca cataagccta acctattgct cacttgcctc ttttattaat ccaaagatag
     5281 ttgtcacaat agtgtgataa ttttttataa aaatgtattt ttgtaactga ccattctaag
     5341 ttgttttgcc atgcagttaa tcattaactc tgacgatatt aaattgttaa aggtattaat
     5401 gtttactctt tttcaaattc attattactg ccatcatttt accatatatt ataataaatt
     5461 tatcttatta agtggctgta cttgattttc actttaaaaa ttatcaaata ttgccatctc
     5521 attttaagta tacaaaatgc aaaacaaccg attcacaagc atatttcaca caagtaaacc
     5581 ggctatttat caacgtatat tcgaagatga attatttcga tagtatctat agaccagacg
     5641 gcattcgcac tttcatagct ataactatac cagcgttttc gtcctcaaag gtgcatacta
     5701 ataaatcgta aacatgactt tatcaaatcg ttctttcttg ttaactaatt tatcaaatgt
     5761 ctccgggcct ttttctaacg gtaaaaaatg agaaataata ggctttacat taatatcttt
     5821 cgtcttcata taatgtaagg ttgccgtcca ctctttgccc ggaaaattac tggacaaaca
     5881 gttccaagag ccacatactg tcaactcgtt acgcagaatt ttttcaaaat gaacgcgatc
     5941 aatctcaata tcatcatatg gtattccgag taataccacc tcgccacctt ttttaggtag
     6001 cgtcaatatt tgaccaatcg taactttagc acctgatgat tctatagcta aatcgatttg
     6061 attggcgtaa tgattttcga tgaatttctc aagattttct tcttttgaat tgattgtttg
     6121 atgtgcgccc aatgatgttg caatatctag tttatgcgca tctatatcta tagcgatgat
     6181 atgtgcagca ccaaatattc gtgcccattg aatagctaac aaacctatac tgccacaccc
     6241 cattactgca acagtcatac caggttgtat attcgattta taaaacccat gcgcaacaac
     6301 ggctgatggc tcaaccattg ctgcttcaat gtaatcaaca ttgtctggaa cctttaaaac
     6361 attttgcgct ggcaatttga catattccgc gaacgatcca ggttcatatg agccaatgac
     6421 gaataacttt tcacatcgtg catattcacc ttttaaacaa tactcgcatt gataacaagg
     6481 tattgctggg caacctgtca ctttgtcgcc cacattaaca tgcgtaacat cacttccaat
     6541 ggcatctact acacctgaaa attcatgacc aaatggcata cctttaatgt atggccccat
     6601 ttttttgtat cgtgacgtgt ctgaaccaca tatgccagtc gctcgtactt taataataac
     6661 gtcattcgca ctttcaatga ctggcttttc attatcctca taccgtaaat cttccacgcc
     6721 atataatttc aatgctttca cttgtaaatc acctcaaatt tgatttaatt cacaactttt
     6781 ttctttttaa aaatacctgt cgcaaaataa cctgcaatga caatggaatt acttacgagt
     6841 aaatgttcca tataaaaatc agtgatttgt cttaatggcc caagcataaa agttagcatt
     6901 gattctacca tattaattct ctccttcatt ttcatctaaa tcgagcggct taagtaatgc
     6961 tttaatacct tcgccggatt ttaaatgttg atacgcctcg tcccatttcg aaatatcata
     7021 tatttttgtc accaaagctt cagcatttac taaaccatcc gccataagtt gcaatgaagg
     7081 ttcccaatct gctggctttt gacttctact accaacaact gttatttctt tttgaatcac
     7141 tttttccata tcaaatggaa tttcagcatc cttaaaaata cctatttgac tgtagaaacc
     7201 ttttttgcgt aaaatatcca aaccttgtcg tgctgctgga actgcacctg aacattcaac
     7261 aacaacatct gcaccgtaac cgtctgtaat tccattgata tacgttttta agtctgtttg
     7321 ttgtaaattg actacataat ccatgtgcaa tgcttctgct ttatctaatc tgactttgtc
     7381 attgtccaat ccagttacca caacagttgc gcctttactt tttaacactt gtgctacaag
     7441 taatccgatt ggcccaggtc ccattacaac tgctacatcg cctgaattga cttgaatctt
     7501 agaaacgcca tgatgtgcac atgctaatgg ttctgtcata gctgcagact gatacgatac
     7561 ttcgtctgga atatgatgca aactttcttc acgtgcaatg acataattag taaatgcgcc
     7621 atcaacttgt gttccaatac cttttcgatg gttgcataaa ttatagtctt ttgatttaca
     7681 gtattcacac tcattacaaa catagaatgt cgtttcagat gtgacacggt caccaacttt
     7741 aaaatcttta acgtctgctc caacttcaac gatttcacca gaaaattcat gacctaatgt
     7801 cactggaaaa ttaactttat aatgaccttc ataagtatga atatctgtgc cacaaattcc
     7861 tgcataatgt actttaatct ttactttatc atctagcggt gttgcaactt ctttatcaag
     7921 aagttctaag ttgccatgtc cttctcttgt ttttactaaa gctttcacca caaacacctc
     7981 gatttttaat tgaatagact aaatagttta aagataagat agttaacgat attaccacct
     8041 tgatcaatac ttgaaatttc agatgaacct tttggcattt gtacattcgt acctttcgcc
     8101 atatctgtga aaatgggtgc tacgtctgtt gcaatatata atgaaattgc aatcataatc
     8161 gtacccacaa tgacagaatg aataatgttt cctcttgctg caccaacaat aaacgcgaca
     8221 acaaatggta tcgttgctaa gtcaccaaaa ggtagtactt ggtttcctgg taaaataacg
     8281 gctaataaaa cagtgatagg tactaaaatt aatgctgtcg aaataaccgc tggatgacct
     8341 aatgctacag ccgcatccaa tccaatataa atttcacgtt cgccaaaacg tttatttagc
     8401 catgttcttg cagactctga aactggcatt aaaccttcca ttaagatttt taccattcta
     8461 ggcattaata ccattactgc agccattgac attcctaaat taatgatgtc tccaggtttg
     8521 taacctgcta acacaccaat acctaaacct aaaattaagc cgacaaatat agactctcca
     8581 aatgcgccaa aacgtttttg aattgtttca ggatcagcat ctaacttatt cagaccgggt
     8641 actttttgta acaatttaac taagtaaata cctggtgcat aagaaattgt acttcctgta
     8701 gcaatcgata cacctggtaa atcaaagaac tcactcatca tcggtgctgt ccaatctgct
     8761 actttcaaac agataatttg gaaaataact gctgctaata acgcttgcca aatactgcct
     8821 gatacggaat aaaccattgc tgctgtaaac gtataatgcc aaaaattcca aatatctaca
     8881 ttcatcgtct ttgtcacttt agttactagc aatacaacgt taactatgat tccgagtgga
     8941 ataataaatg ctgcgacaga tgatgcccaa gcgatagatg atgttgctgg ccaacctaca
     9001 tcaatcacat tcagactgac gcctaaattt ttaaccatcg cttgtgctgc tggccctaaa
     9061 tttttaacta ataaatcgat gactaagaaa atccctacaa aagccacacc tattgttaaa
     9121 ccagacctaa atgccgctcc aattttctgc ctaaagaata ggccaagcaa gaatatgaca
     9181 accggtaaaa taacagttgc acctaaatct aaaaatcccc ttacaaaatc agtgaagtaa
     9241 ctcatattta aaccctccct gttatatatg cattgtcacg atactttccg attgtgatta
     9301 catttgacgt tacagtcatt tcaacgacaa cccttgctac attcgactgc agtccttttg
     9361 aattacagtc actgcgtttc tatgtcatca acaatcattt gtcgtgatag tcatttatat
     9421 gcaatttgca tatatttata tgttattgac ccacgttaca tatcaattcc gttatttttg
     9481 taactctgtt aagatttgtt gttttgtttc ttcaatacca ataccagtta agaaattacg
     9541 tgcgttgata actgggaatt tatattcttt ttttgtcatt gcagttgtaa ctaataaatc
     9601 tgcagtgtct tcataaggtc caacttctgt aattttgatt tgtttaatat atactttaat
     9661 attgtgttcc tttgccattt cctcaattgc attatttact actgttgacg ttgcaatacc
     9721 tgcaccacac gctactaata cttgtttcat tttcaattcc tccaattaat ttttagttat
     9781 attccaaata atcattgatt agtgttgcta aaattgtttc atctttcgtt cgtagaatct
     9841 gctccaattt ttcttcactt tgaaaaattt gcatcaactg ttgtaacagc ttaagttgat
     9901 catctacttt attcattgct aacataaaaa cgattttcac ttctgtctgt tgatcaagtg
     9961 ttcccatttc aataaacggc acttcttttt ctagaacagc cacacctatc gttctatggt
    10021 taatatgttc gacatctgta tgcggtatag cgaccgaaca tagatgcgtt ggtaaaccag
    10081 tagcaaattc tttttctctg tcgatgactg catctttaaa cgttgacttc acgaacccat
    10141 tttgaaataa cacatctgac atttgtgaca atacggattc tttatcagtt gccgacaaat
    10201 tgagcattat attttcttta tgcactaatt gctgtcccat ccatttcccc tcgcttcttt
    10261 atttgaataa ttttttaaaa tctcatttac atcagaattt ttgcgacttt gtatgatgcg
    10321 cttaattgcg tcattgtctt gcgccacatc tctcaattgt agtaacgctc ttaagtgtgt
    10381 cactttatca acagcagcaa taggtacaat aatatggatt gctgttccat ctgacatgta
    10441 tattggttct tgtaatatca acatactcat cgctgtttta tgtacatgct tttcagagta
    10501 tgcatgtgga atagcaatat tttgcatgat aaccatatac gaatcatcaa atacctgttg
    10561 catctcatga atatatttaa tatcaataaa atgattagca actaacacat cacttgcttt
    10621 agcaatagct tcatcaatat tttcaacatg atgcattctt ttcacgtgcc ttgccggtat
    10681 caagtcagct aaatctaatg tcttattttg tgtgacaatc gatccattaa tggttgaaat
    10741 tgaattataa ttggcaataa aatcttctaa accatcacgt agttctgtaa tgtcattaac
    10801 tgtcgttgtg cgttcaatta atgccattaa cttgtttatt tccttatcaa tgtcagccga
    10861 ttccttatta atgtacttca tcacatcttt acgtaacttt cgttgctcat tttcagataa
    10921 agctactttt gtgataaata attttttatg tgttaggaca aacattggtg aaaagacgat
    10981 gtcataatct aatgtgtaat tttcaaatgt tctaagtgaa atcgcatcta agaaaataat
    11041 ttctggaaat aagtttcgca actcgtataa catcatttgt gatactgacg tgccttgtgt
    11101 acacacgata atagctttta tcttgccatc gaagttttca tcttgacgtc tcaaactacc
    11161 tccgaacaac atggttaaat atgctatttc attatcaggc aacgattttc cgaaatattc
    11221 agttaacgat tgacatgatt gtttcaccat atgaaataag gattgataat ttccttgtaa
    11281 aggatttatt aattcatcac gatccgttaa gttatattta atcctataaa aagcaggcgt
    11341 taaatgtaac aagagttgct gtgataattt ctccttatct tcaatgttaa taaaagtgat
    11401 ttgttcaaaa tggtgaatca tttgagcgat ggccatcgtt aaattcgata tgttatctga
    11461 ttcctgcaaa tcagtccatt gcacacttgt tgaaagtaag tgtaatgtca aatataactt
    11521 ttccgcttct ggcaaatccg gctcatgttg cgtcataatc tccgttgctt gatattcttt
    11581 cgtatccctc aaatactgat aattaatatt taatggattc atcacatgac cactttgaat
    11641 tcgtctacga atcacacaaa ggacataagg caatgaacta agtgatttgt ctataaagcg
    11701 actcttcaaa aattgttcta cctgtttgat cttgtctttt tgatatgcga tatcttcgaa
    11761 tgttaagttg agcgccttta aaacttcact tttagtaata tcatgattca acctttgatc
    11821 aatcaactta atgaagaaac ggcgaacttc aaattcatca ccaacaattt cataaccatg
    11881 ctttcgagaa tacttaagtg acaaaccatg atcttccaat tgctctttca catgatttat
    11941 atcgtgaatg acagtatttt tactgacttg taaatcaatt gaaaaatggt ttagagacat
    12001 tgcattttcc ttactaaaaa gcatgagcat taaataataa cgacgtgttt ctatgctaaa
    12061 aatgacattg ttgccgttta acatttgctg ctccgataca tctcgcttga ataacgtcat
    12121 gatttcagaa cttacaataa aatttccttg gcttgttctt tcaagttttg gataaccctc
    12181 ttgttcaagc cacaaattga ttttttgaat gcgatatcct agttgtctac gagacaaacc
    12241 aaatatcgat tcaagttctt taccatgaat agtaggattc aatacaattt ctctgagtat
    12301 ttgctgatta tccccgttca tgttcgatcc tcctttgagc atctgagctc aatttatcat
    12361 caatcgtcac accgatgtac acactttgaa cacatatttt caaaatgagc atgtacatca
    12421 ttgtgatgtt ttaacaacat ttcaattata tctatatttt ttgtgatttt aatcttttaa
    12481 aataaatcaa ttgaaatttt tacatatatt tttgtgtttt atgttttttg aagcattttt
    12541 aacatacata tctcaatcat tatcaaattg tcatgaccaa tgtaatccaa tacaaaaacc
    12601 ctaaggacgc ttatatcagg cgccttaggg ctaactgtat ctatttaatt aagtattatt
    12661 attcgtaagt acgtaactta taatctatca agttccacac ttcttcaaca tcaactgctg
    12721 tagcaaaata agcattggca ggcttacctg taacatgatt taaatcgaca gccatagtgc
    12781 cataagttag tgaactttga tgttcaatgt cgatattaac gggtaccatt gtaaacaatt
    12841 ctggttgtaa caaatacaaa attgtacaag catcatgtat tggaccacca tccatattaa
    12901 agtgagtctt gtatgtcttc ttaaagaatt gcaataattc tacgacgaac tgtgcaacag
    12961 gattattgat actttcaaag cgttcaatca cgtgattgtc ggctaaaact tgatgtgtta
    13021 catctaaacc aaacacattt atagtaatcc cactttcaaa aacacgcttc gctgcttcag
    13081 catctaccca aatattgaat tctgctgtag gcgtccaatt tccaaatgta ccaccaccca
    13141 tcaaagtaat agattcaata tgctcagcga ttcttggctc acgaatcaat gccgttgcta
    13201 cattcgtaag aggacctgtc gctacaattg ttacaggtgt atcactcgtc atcactttgt
    13261 ttataatcac atctgatgct ggcattgcaa ctgcttgacg tgatggtgtc gacggtagtt
    13321 tcggaccatc taatccagat tccccatgta tttcagaagc aaaggcagct gatttaatta
    13381 acggcctgtc cgcacctttc gctactgcta tatcttgacg tcccataata tccaatacgt
    13441 tcaaggcgtt tgtcgtattc ttgtcaactg attgattacc tgcgactgtt gttacagcta
    13501 atatctctag tggactgtca attgcccccg ctaaaattaa tgctattgca tcatcgtgtc
    13561 ctggatcaca atccataata atctttcttt tcatttatat atccaccttt cttaagttgt
    13621 tatcgatagc ttatgtatat ttatttatgt ggtgaatcat gtttattttg aaaaatagtt
    13681 ttaactttct catatttttg gatacaaaca ctatttatct attttatggc ttataaattt
    13741 atccgatatg ccttatcaac ctacctcgct aaaaatagga tgtctacata tctataccga
    13801 cttttgtcaa ctcattttca caacaatata aacagcaatt tatatgattg ttacatgatt
    13861 caaacaattt ttatgaaaaa tattttcata cacagaatat atattgatat taaatttctc
    13921 aaaagctata ttgagaataa ttaggaggga tgttgatgaa atctttattt gaaaaagcac
    13981 agcagttcgg caagtccttt atgctaccta tcgcaatctt accagctgca ggtctattgt
    14041 tgggtatcgg tggtgcatta agtaatccaa acaccgttaa agcataccct attttagata
    14101 ttaccttatt acaaaatatt tttacattaa tgtcagctgc aggtagtatt gttttccaaa
    14161 atttaccagt catctttgca attggtgtcg caatcggatt atctagaagc gataaaggta
    14221 ctgcaggttt agctgcacta ctcggtttct taattatgaa cgcaactatg aatggcttat
    14281 taactatcac gggcacattg gcaaaagatc agcttgcaca aaatggacaa ggcatggtgc
    14341 tcggtataca aacggttgaa accggtgttt ttggcgggat tatcacaggt attatgaccg
    14401 caatacttca caacaaatat cacaaagtgg tattaccacc gtatttaggt ttctttggtg
    14461 gctctagatt tgtccctatt gtcacagcat ttgccgcaat ctttttaggt gtattgatgt
    14521 ttttcatttg gccaagcata caagccggca tttatcatgt tggtggattt gtaacgaaaa
    14581 caggtgccat cggtactttt gtttatggct tcatcttaag attgttaggt ccactcggtt
    14641 tacatcatat tttttactta ccgttttggc agacggcact tggtggtact ttagaagtca
    14701 aaggacactt agttcaaggt acgcagaaca tcttctttgc tcaacttggt gatccagatg
    14761 tgacgaagta ttattcaggt gtgtcacgct ttatgtcagg ccgttttatt acgatgatgt
    14821 tcggcttatg tggtgccgca cttgcaattt atcacacagc taaacctgaa cataaaaaag
    14881 ttgtcggcgg tttaatgtta tccgctgcac tcacttcatt tttaacaggt attaccgaac
    14941 ctttagagtt tagtttcttg tttgtcgcac ctattcttta tgtaatccat gccttctttg
    15001 atggattagc atttatgatg tcagacattt tcaacattac aattggtcaa accttcagtg
    15061 gaggctttat cgatttctta ctttttggtg tgctacaagg taatagtaaa acaaactacc
    15121 tatacgtcat acctattgga attgtgtggt tctgtttgta ttacatcgtt ttcagattct
    15181 taattacgaa atttaatttc aaaacacctg gtcgagaaga taaagctgca gcacaacaag
    15241 ttgaggctac tgaaagagca caaactattg ttgctggttt gggaggcaaa gataacattg
    15301 aaatcgttga ctgttgtgca acgagactac gcgtcacact tcatcaaaat gacaaagtcg
    15361 ataaagcatt actcgaaagt actggtgcca aaggtgtaat ccagcaaggc actggtgtgc
    15421 aagtaattta tgggcctcac gttacagtta tcaaaaatga aattgaagaa ttgctcgggg
    15481 attaagacta accgaaatat caacagaact aatggcaacg atgtacgaag taagaagtga
    15541 catcgttgct tttattttta atgttacatt tgaagcatta agttcatcat gcactgtagt
    15601 gagcccgcaa atcgcctctg ctagacaatc atcttaatgc tatgattaaa gcttaagtgc
    15661 cagatttgaa tttaatttca acaacgactt tcactacatt aaaaataggg ctactcgaca
    15721 catatagttg tatcaaatag ccctttatac aatttttggg gtaaggtttt acaatttttg
    15781 gaatggtata gattttataa aaagttattt aagttcttct gcttcagcca taatatcttt
    15841 taatgtttta gctgaatgtg cgaacttgct ttgttcttcg tcgtttaatg ggatttctac
    15901 gacgttgcga ataccgtttc tattgatgac tgctggaaca ccaatataaa catcttcttc
    15961 atcatattcg ccttctaata atgctgatac agtcaatacg gcatcttcat ttctgaaaat
    16021 cgcttcagta attctagcta atcccattgc aacaccataa taagtggcac ctttagcttg
    16081 aataatgtca tatgctgcat cacgtgtttg aacaaaaatt tgttcaattt gcgctttgcc
    16141 ctcaggacgt tgttcaagta atgtcttcaa aggttgaccc gcaatattag cgtgtgacca
    16201 tactggtaat tcagtgtcac catgttcacc aataatttga gcatcgacgc tacgtggcgc
    16261 aacatcgaac gcttcgctta acaataatct aaagcgtgca gagtctaaaa ttgtaccaga
    16321 acctataaca cgttctttag gtaaaccaga gaatttccat gttgcatacg ctaaaatatc
    16381 aacaggattt gtagctacca agaaaatacc atcaaatttt gatgccatta cttcaccaac
    16441 aattgatttg aatattttca agtttttaga tactaaatct aaacgtgttt ctccaggttt
    16501 ttgtgcagca ccagcacaga tgacaactag atccgcatca tgacaatcac tgtattcgcc
    16561 agctttcaca cgaactgttg ttggagaata tggtgtggca tgttttaaat ccataacatc
    16621 tcctcgaact ttttcagtgt ctaaatcaat gatgactaat tcatcaacaa tgctttggtt
    16681 cactaatgaa aatgcgtagc ttgaacctac tgcaccatta cctattaata caactttgtt
    16741 ccctttaaat ttgttcatta caaaaactcc cttatgatta attcactaac atacgtgtag
    16801 cttcaaatat gttagtttaa tgctgcttat tgacgataca aaagcaaata aacatctctt
    16861 ttattttcaa cgcataactt aaaaggtcat gtgtcatccg cttttaagtt tgtgatttat
    16921 ttcacatata aaatgtaaca tgcattaagt actgggtcaa tattaaattg tgatttattt
    16981 cacattttat tttaattttt acaccttttt aatttgtata cgattacatc ttagatgtct
    17041 ttagtcttcg tacttcgcca gtgattattt acactttcac atttttatta tcatgtttac
    17101 ttttttctaa gaaaacaaca atgttttttg aattagtcaa ataaatgcgc gcaatcgtcg
    17161 gtgtgcaaac agacaattgt acacaatgct tattgataag tatttaaaaa attaaaaatg
    17221 tcatacaatt atcaaatttg ccattttatt tatattttct caaaccaatt aattgaatat
    17281 cgaaattttt agtagaataa tcaaaataga cagattaaag gaggagtatc atgcttacag
    17341 aacaagagaa agacattatc aaacaaacgg tgcctttact taaagagaaa gggacagaaa
    17401 ttacgtcaat cttttatcca aaaatgttta aagcgcatcc tgaactttta aacatgttta
    17461 atcaaacgaa ccaaaaacga ggcatgcaat cttcagcatt agcacaagct gtaatggccg
    17521 ctgcggttaa tatcgataac ttaagtgtta ttaaaccagt cattatgcca gtcgcatata
    17581 aacactgtgc attacaagtt tatgctgaac attatccaat tgtgggggaa aatttattaa
    17641 aagcaatcca agacgtgaca ggattagaag aaaatgatcc tgtcattcaa gcttgggcaa
    17701 aagcatatgg cgttattgcg gatgtgttca tccaaattga aaaagaaatt tatgatcaaa
    17761 tgatgtggat tggttttaaa ccattcaaaa ttaccaatat taaacaagaa tctgaagaca
    17821 ttaaatcatt tacagttgaa actgaagaat atgactttag tgaatttaca ccaggccaat
    17881 acatcacagt tgatgtttct agtgataaac tttcatatag agctaaacgt cactattcta
    17941 tcgtatcagg tgaaaaaaac catttaactt ttggcgttaa acgtgatgtc acaacagaac
    18001 atgaaggcga agtttcaaca attttacacg atgagattaa agaaggcgat atgattaatt
    18061 tagctgcgcc tgtaggtgga ttcgtattag agaatacgac tgaaccacaa cttttcttag
    18121 gttcaggtat tggtgttaca cctttagtag ctatgtatga agctgcctct gccaaaggtt
    18181 tagatacaca gatggttcaa gttgctgaaa atgaacaaca tttacctttc aaagacaact
    18241 tcaacagtat cgcaagccat catgacaacg ctaaattata tacacactta aaagataaac
    18301 aaggctatat tggtgctgaa gaattacaag tatttttagc aaataaacct gaaatttata
    18361 tctgtggtgg tacaaaattc ttacaatcta tgatcgaagc acttaaatct ttaaattacg
    18421 atatggattg cgtacactac gaaacattta ttccaagact aagcgttgca gtataattga
    18481 gaaaagaggt ttagtgctca tatgaaactt aatcaacgtt acgtaaaagt atttgcatta
    18541 tatttcgcga gtattgttac cgcaaatatt gttgtcaaaa ataacaattt aatcaaaaca
    18601 ttgattcaaa cacttgccgg ttacaccgtc tttgcagttg gtttgaagta tttaactaaa
    18661 cgtaaaaata aatgacatct ataaaaaaca gctatgactt tactttgtca tggctgtttt
    18721 ttaattgaga gtgagacaga aatgatattt tcgcaaaatt tatttggtcg tcccaccaca
    18781 acatgcattg atgtatgctc actgaatttc ataagaaagg agttcacaag atgaccgtag
    18841 atattggacg gatttatgac aataaagata ataccgacgc tattcgtatc ctagtcgata
    18901 gagtctggcc gagaggtatt tcgaaaagaa ctgctaacct agattattgg ttaaaagaca
    18961 ttgccccttc tactgagttg cgacaatggt tccaacatga tcctaaactt tttggagctt
    19021 ttaaagaaaa atatgaaaaa gaattacgtg atcaggatgc gcaaaaagat gcttttgaaa
    19081 aattaaagga tattgtaaat cagcataatc atgttctatt gttatataca gcaaaagata
    19141 ctaaacataa ccaagctgta gtactacagc agttgctcaa tacttagtta tatatgttta
    19201 tcgtgaatca ctataaatat cgctaacttc atttctaata tttattttta aaaagcataa
    19261 ttactacaat taattgaact ttaataatta ctaaacttga aaaacatttt actttaacaa
    19321 aataaagttt taaaatatta attgttgggt tttacatagt aattagaaag agagtgttaa
    19381 tgaaatgaag tttaaaagac tagcaactat attttcagca gtattagtgt tatcaggatg
    19441 tggctcaatg cattcatctg gcaaagactt aaatatttcg ttaccgttaa aaacaaagtc
    19501 tattgcacct tatgaaacag atgtaccagt taagattggt gctgcagaat cattatttaa
    19561 aacaaatgat caaggaaaaa tagaaaaagc attagtgaaa tcgtatcatc aaccaaatga
    19621 tacaacgtta gatatagaat taaaagataa tattaaattt caaaacggtc aaaaattgac
    19681 tgcagaaaaa gtgaaatcta gccttgaaaa tagcatgaaa aaaagcgact tggtcaaata
    19741 ttcattacca atatcatcaa ttaccgctaa aggtcaaaaa ctgacaatta aaaccaactc
    19801 cgcttaccct gaacttgtat ctgaattagc taatcctttt atggcaattt atgatacaga
    19861 tgctaaatca gatgttaatc aaactcctgt tggtacaggc ccttaccaaa taaaagatta
    19921 taagcaatct cgaaaaatat cattgtcgaa ttttaaggac tattggcaag gtaaacccaa
    19981 acttgatcat attactgtga cgtaccaaga agacggcaat aatcgcgtca gaaatttaga
    20041 atctcaaaaa gatgatttaa taactgatgt cccagttaat aaagttcaag acatagaaaa
    20101 taatcaaaat ttaaaagtgt caaaagaatc tggatttaga acttctttac ttatgtataa
    20161 tcatactaat aaaaaaatga ctaaatccgt tcgtgaagca ttagatcata tcattgatag
    20221 acaaggtatt gcagatcata tttatcaagg ttatgcgaaa cctgcaacga gtccatttaa
    20281 tgataaaatt ccatatatta aagaacctaa gttaaccaaa caaaatatcg aacaagcaaa
    20341 aacgttatta gctaaagatg gttatacaaa agaacaccct ttaaaaatta agttaattac
    20401 gtatgacggt cgtccagagc tatcgaaaat tgcgcaagta ttacaatcag atgctaaaaa
    20461 agcaaatatt gaaatcgaca ttaaaagtgt tgatgacata gaaggttact taaaagaccg
    20521 ttctgcatgg gatgcaacga tgtatagttt tgggacaatc cctcgcggtg atacaggtta
    20581 tttctttaat caagcatata aaaaagatgg tgctattaat aaaggagact acaataatag
    20641 caacgtcgat gatttaatta atcaattgaa tcatactgta gatgttaagg aacgtcacaa
    20701 tatttctaat gatattatta aattatcaag tcgagatgta ccaaacagtt atattgctta
    20761 caacgatcaa atagtcgctg ctaactctaa ggttaaaaat tataaagtaa cacctgaagg
    20821 tatctattta atcgattatc gaacaacgat tgaacgataa gggagtgtgg gacattgatt
    20881 tatgtctcag ccctgcttca actataaaaa ccacgccgaa gtcatccaat atgattatgg
    20941 cgtggtttaa tttgcttata tgattgtatt aatttctgtt tgatgaattt caataaaatg
    21001 attattacat actttcagtg aatcatgttc attttatata aatctaattg tcactatttg
    21061 tttaacgtga aacacttttt taactccaaa aagtattcct attccactct tttaaagaca
    21121 aattataaaa atagcttatg tcacaaacat atgagcaaca taagctttaa ttattattcg
    21181 tctacttttt tctgttgttc tttaaattct ttctgcagta aattcacttg ttttaaacct
    21241 gcccccatta aaatgaatac aagtatccac acaataacga ttaaaataaa tattttcaac
    21301 gtatcactgc caagtacagt taaagatgta tcccaaatgc catgtaaaac aacggctgat
    21361 aaaaagaata ttaaaaagcg tttatcaaaa atatctttga attcaaagcc atgctgttct
    21421 ttcgcaataa ctattgcagc accaacaatc gctgaccaaa ctaaatgacc accaatcgca
    21481 gtccacgcac gtgtgaagac aatatctaat aatgggacat tttctcctaa agcgaaattc
    21541 aaaatataac ctgctgattc aaaaactgcg aaccctgcac caatagcagc accgattaat
    21601 aatccattca aaatcttatt tgttttcaat ttattgacga aataaacaat aatgagtgct
    21661 tttccagttt cttcaactaa tcctactaaa aatgcatcga aaaatgttaa agaaccaaac
    21721 ctttcgaatt gatcactaaa aacgacaaat ctatataata ccatcgtact tagtaatgaa
    21781 aatacgccgc caataaagaa catgataata acttcaaaaa tgctaatatt tttaaacgca
    21841 tttgattcat aaaagaagaa caaacccgat aatggtactg ttaaagcccc tataaaaatg
    21901 agacccggaa tcgcgttagt gttattaaaa attgctgcca tgacccataa tccaataaat
    21961 gttactgtga aagcgatgaa tactcgagaa aagagccatg gcttccccca ttcttctgat
    22021 atgtcacaaa ttgctggcgt agttttagca gtacccgcta taaatatttc atctgcttcg
    22081 tttttagtat gcggcttaaa tacttcagaa agcatatctt ttaaattaag atttaagctt
    22141 tcatcattgc ccgctagtct accgattgat ttggttgcat tatcaaataa acttcttgct
    22201 tcgtttttta aatctgtatt ttgcgctgtt tcatgcgctt tagcttccat ttgtttatcg
    22261 atagattcat tatgtatgtc ggacgtctta gttgattgtg tacttgctga tgacatcccc
    22321 tcttcacctg tattaggtgc aactgattgc gtttgtgttg tctcttcttt ttctgtattt
    22381 cttcgatgat taagaccttc catagactta cctactttca cttttcataa taaaactatt
    22441 ttaatttaat attgatggat tttccatatt ttaatataaa tactttgttc gattgggcac
    22501 tttgtatttc atatttaaaa atcaaaacct atttaacttt ttatattccg aacaatattt
    22561 ttatataagt attttaatga tattcaattc attacaaacc cttgatatta caattgttat
    22621 cgaccatcaa aatgtgtcta tactgtgtct attatcagaa taaaaagata tttctaattg
    22681 attttaaacg tcgttatgtt atatttcttg taagggttta caaaaagtgt aaaaacgcta
    22741 tattttatca aatgtgatgg aggtgacttg cttgaaacaa atcacatggc acgacttaca
    22801 acatatcatt aaagatggtg atgtgattgg tttaccagca ttagctgtag ccaacttacc
    22861 cgccgaagtt ctacgtgctg tgttagcgca acatgacaca tatcatacgc ccaatgattt
    22921 aacgtttata ttagcgaatg atattcatag tttaggtgcc gcaccggatt tagatgattt
    22981 tatagaacgt cgcatgatta aacgtgtcat tatgagtatt ttaacggctt cttccaaaac
    23041 ggcacaagca atgaaaaata atgacattga agcttatttt ttaccacaag gtatcattgc
    23101 aactcattat cgtcagagta atcaattatt acctggagtt attactaaaa tcggattaaa
    23161 cacagctgtt gatcctagat acggtggcgg taaagtaaat acacgaacaa cggatgattt
    23221 agtttcatta gtaaccatca acgatgaaac atacttacat tacacattcc ctagcgttga
    23281 tgtggcacta ctgagaggaa catacgcaga tcaacaaggt aacatttatt taactcaaga
    23341 agcgtacttg agcgagtgtt atcatgtcgc attaaacgcg aaagccaatc atgggaaagt
    23401 tattgtacaa gttaaagctt tagttgacga ctatcaacta aaaccgaatg aagttgttat
    23461 cccaggaaat cttgtcgatt atgtatacgt cacagaagat gaaaagaatc accgccaagt
    23521 aattcaaagt cattatttac cagccttgtc tggagaagaa cgaattgatg gaatacctga
    23581 acccgcatta ccttttaata gtcgcaaatt gattctccga cgtgctgctc agtttttaac
    23641 ttatggcgat acaattagca tcggttatgg catcaataat gaactctcta atttattgca
    23701 tgaagaatgt gttgaacatg atgtgcaacc gattttagat gttggcattt tcggtggatt
    23761 cgttgggagt cgtgaacatt ttggtatgaa ttacaatgca gatgtgcgca tgcctcatga
    23821 tcgagcatgg gattttattt ataacaatgg tgtatcagtt gcctatctta gctttgctga
    23881 ggttgatcaa tacggcaatg tcaacgtgtc ttacttcaat gaccgactaa atggatgtgg
    23941 tggctttata gacattacgc aatctgtaaa taaaattatc ttttcaggta cttttgtagc
    24001 tggcagtcat gtctcatgcc ataatcaacg attaaacatt gaaactgaag gacaaaacca
    24061 gaaatttgta tcagatgtga gccatatcga ctttaatgca caatattcac aatcactcga
    24121 gcaagaaatc tattttgtta ctgatcgtgc agtattcgaa ctcaccaatc aaggcttgaa
    24181 actaattgaa attgcaccag gtcttgattt gcataaagat attttgaatc aaatggcttt
    24241 taaaccaatt attgctgatc atttaaaatt aattgatacc agcatttaca aagaaaaatg
    24301 gggacaactt aaacaatcaa ttcataaagt atgaaagtga agctaaaggg ggtatactaa
    24361 tgaatttcga ttggataaaa acgcgttcag acttcgatga tgacaagcct gccgttattg
    24421 atcacgcaaa acaaacatct tggacatacc aacaactcaa tgcacgcgct gataatatgg
    24481 cacattattt aacatctcaa ggtgttaaaa aaggcgatgt tatcggtatt tttgcgccaa
    24541 atgatattgc aatattagat ttattgtttg cttgttttaa aacaggtgcg gtttttttac
    24601 cattgaattg gcggcttaac ccaaaagaaa ttgcagccat tgtggaagat gcacaattaa
    24661 aactgctctt ctatgctgaa aaacatttaa gttcactcac cgatattgac caaaacttat
    24721 tgcatatgga tattgatgtg gcgcaatatg atgaaatcgt aaatccagat tatcaccaac
    24781 cttttcaagc aacacctgtt gaaccacaag atcttgcagc attaatttat acaagtggta
    24841 caaccggatc acctaaaggt gtgatgttct catatgaatc atttgttcat aacggtgcaa
    24901 acttagagct gacgtataag ttcaattcaa actatattac gattgtatca acaccaatgt
    24961 tccatgtttt agggtttaac gatactgtat taccagtatt aatgtcaggt ggcacactta
    25021 ttcttcaacg ttactttaac ggcgaagaat tgaatgacat gattgcacaa tatcacccta
    25081 catttattat catgattccg acaatgtatt atagtacgct acgtgctagt aattttaatc
    25141 ctgaaaattt tagagctatg gattatatca tccaaggtgg ttcacaacca ttaccaagta
    25201 tccaagcagc ttttaaacaa tatggcataa acattattaa cggctatggt ttaactgaag
    25261 cacctcttgt acttgttaat acaccggaaa attcaaaacg taagccaatg agtattggta
    25321 aagcagtcat gttcgttgat gcacgtatcc ttgatgataa cggtgaggaa gtacctactg
    25381 gtgagattgg cgaacttgca attaaggcta aaaatgtcac gccaggatat tggaataaac
    25441 cagcagagac tgccaaagca tttcatggtc gatatttatt aactggtgac ttagcgaaga
    25501 tggacaacga tggcgatata tttattattg accgcaaaaa agaattaatc ataactggtg
    25561 gcgaaaatgt cttaccatcc gaagtcgaaa atgctttagc tgagcatcca ctagtagacc
    25621 ggtgtgtggt cgttggctat gatcatccaa aatatggtga atcaattgct gcagccatta
    25681 tacttcgcga agatgaacct cattacgctg aaattttaaa tcaacatatg cgaagtcgtt
    25741 tagcaggtta taaagtccca agaatgtatg taccagtgac acatatgccg ttaaacagta
    25801 cgcagaaacc agataaactt gcgattcgac aaatgatgaa tgacaaagtc tcgcaaacac
    25861 tttaaaggtg ataaaaattt ttgacattta gtgtaagcgt ttacaaataa agcgtgttgt
    25921 ttttgaatta aatgcatttc acattagtac tcatattatt tttaggagga atttatatga
    25981 catttgaaaa agaaacggtc ttaaaaacat tatttcctga agatgtactt agtattgcta
    26041 aaggtttaac agacggtgaa gtcgaatttt tacaacaagt agattcattg ctagaaagta
    26101 agtaccgtga aaatattaat caacattgga tagacgctac tgtacccgag gactatttta
    26161 aagatctgag agaattaaat tattttaaca atccattact ttacaaggat cgtccaaacg
    26221 ccaaaatgcc tagtcaacta tttcagtttt tcatgtctta cctactcgcg cgatttgata
    26281 tttccttagc taccctactc ggtgttcacc aaggtttagg gcataacact ttctatttcg
    26341 gaggtagcaa agaacaaatt gcgaaatatg tacctaaatt acagtcacat gaactgcgta
    26401 catgctttgc tttaactgaa ccagaacacg gttcggacgt tgcgggaggt cttgaaacag
    26461 tcgctgaacg ccaaggcgat acttgggtta tcaatggtga aaagaaatgg attggtggtg
    26521 cacatgtatc tgatgtcatt ccagtattcg cagtaaataa agaaactggc aaaccccatt
    26581 gctttgtagt cagaccagaa caagatggcg tcgatattga agtcattgat aataaaatcg
    26641 cacttcgcat tgttcctaac gccctaatta aattaactaa tgtcaaagta gatgaagcgg
    26701 atcgcttaca aaacataaca agctttaaag atattgccaa aattctttat tcaacgagag
    26761 caggcgttgc ttatatggct acaggtggta tggctggcgc tttacgtgcc acattagatt
    26821 atgtcactga gcgtaagcaa ttcggcaaac caattagtaa atatcagtta atacaagaaa
    26881 agctagcaat gatgcaaggt aatttagctc aagcaatggc aacatgtgct caattagcta
    26941 atatgcaagc acatggtgaa tatgacgagg ttgcaacttc aacggcgaag atgatgaatg
    27001 ccttacgttt gcgtgagaca gtagctatgg gccgcggtat tacaggtggt aatggcatac
    27061 tagctgacga ttatgatatt gcacgtttct tctctgatgc agaagcgatt tacacgtacg
    27121 aaggtacaca tgaaattaat gccttagtaa ttggacgcgc tttgactgga gattctgctt
    27181 tcgtataaat agcaaataat tatatgagat gcattaattt cactaaaaaa gacttatttt
    27241 aagcataaag ctttttcctt aaataagagg ctaagatgac tgtcaaagat acttaattaa
    27301 ttttataaaa tagcaacgtt attccaatta tcttaatggt tatcttatcc tcaactaaat
    27361 tggaggaatc actatgacaa ttaataaagt aaccgttctt ggcgcaggca caatgggcgc
    27421 tcaactggca gcactttttg tgaatgctgg acttaaagta aaactattag atattgtagt
    27481 ggacaaaaac gatccaaatc tcattgcgaa aaaatcttac gataaaatta cagataagaa
    27541 acggccgcta ctattcgact taaatctagc gagtcattta acatatggta attttgatga
    27601 tgacttggta aatgatgatg ctgatttata tatcgaagca gtcaaagaag atattgaaat
    27661 taagcatgct gtttggcaac aagttctaca acatgctaaa gaagatgctt tattcgctac
    27721 aaatacatca ggtattccaa ttaatgcgat tgctcaagca tttaacgaga aggatcaaga
    27781 acgattcttt ggtctacatt tctttaaccc accacgtatt atgaaattag tggagttaat
    27841 acctacgtca cacacgaagg aatctattat attagatgta aaaaatttcg cgcaaaatgt
    27901 gttaggtaaa ggtgtcattg tcgtcaatga tgtgcctggc tttgtcgcaa atagagtcgg
    27961 cacgcaaaca atgaatgata ttatgtatcg cgccgagcaa cacaagataa gcattgtaga
    28021 tgtggatgct ttaactgggc aagcgattgg tcgtcctaaa acaggtacat atgcgctatc
    28081 tgacctagtc ggtttagata ttgcagtgtc tgtaattaaa ggcatgcaac aagtacctga
    28141 agaaacacct tattttcatg atgtcaaaat tgtaaatacg ttgtttgaca atggcgcact
    28201 cggacgtaaa acgaaacaag gattttacaa aaaggataaa gaaactaaag ctcgacttgt
    28261 ttacgatgtt gaaaaacaag attatgtacc tgtatcgcaa ccacaattac caattttaaa
    28321 tgaatttaat aaagacttag tgcataacct tgatgtcata ttcaatgcgc aagacgaagc
    28381 gggactattt ttatgggaga cattacgtaa taatttctat tactctgcta tcaatgtacc
    28441 taaagctacc gatgatttcc gagacataga ccgtgcgctt gtctgggggt tcaactggaa
    28501 acttggtcca ttccaattat gggatgcaat gggatacgaa cgtgttaaaa cacgtatgga
    28561 agacgaactt ggagacttac cacaatggat tagtgattta gatggtggct tttataaaca
    28621 agatgagacc attgaatatg caacacctgt ttctcacttc gtaaaagatg aactttggga
    28681 taaaggtgat gccaaacttt ccgtaactca tgatgatcaa ctgttactga aattacaaag
    28741 taaaaataat gtcattaccg atgaattcaa cgatgcgtta gttgatgcca ttgatttact
    28801 ggaaaatgac cattacacaa gtatggtcat ttatgcagat ggtaataatt tcagtgtggg
    28861 tgctaacctt ttcttaatga aaaaggcgca tgaagacggt cttgtagatg atgtcgttgc
    28921 acaatcaatt gataaattac attatagctt taatcgtttg aagtatagtt tgaaaccagt
    28981 agtcacagct gttcaaggtc gtgccttagg cggtggctgt gagcttgtac tttactcacc
    29041 tattgttgtc gctgcaagtg aaacatatat aggtcttgtt gaagcaggcg ttggcttatt
    29101 accgagtggc ggtggccttg cagaaatggc tgatcgcata ttacgcacat cgcataagtt
    29161 tgatgacaaa caagcttcca tgacaaaagt actgacgaat atcgcatttg cgaaagtctc
    29221 tacaaatgcc tttgaagcac gtcgttatgg ttatttacgt gatacagata cgattatttt
    29281 caatacagca caacgtgtcg aagttgcgct caaacgtgcg aaatatgaag cagaaacaaa
    29341 ctatattccg aatcctagac atcaatatat cgctttaggt gaagacttca aagcattgat
    29401 ccaaggacaa ttagatgcgc aaagacgggg gcattttatt agcgaccatg attatcatat
    29461 cgccttaaat atcgccacaa ttttagcggg tggtgattta ccaagaaata catttataaa
    29521 tcaacgttac attcaatcgt tggagaaaat tggctttatt gacttactaa aatctaaaaa
    29581 atcatatgaa agaattgcac atatgttaaa aactggtaag ccattacgta attaaaagat
    29641 agtcattaag aaaggatgat aaccatgcaa gaagcataca ttgtagctta tgggcgttca
    29701 gccgcagcga aagcaaagca aggcgcatta ttccacgaaa gacctgatga tgtcgcagcc
    29761 aaagtattac aaggcgtatt gaaacgtatt gacggaaaat tcaataagaa tatgattgaa
    29821 gatgtcattg ttggtacggc ttttccagaa ggattacaag gacaaaacat tgcacgaacg
    29881 attgcattgc ttacgggatt atctgacacg gtaccgggtc aaacagtgaa tcgctactgc
    29941 tcatcaggat tacaaaccat cgcgattgca gccaatcaaa ttatggctgg tcaaggagat
    30001 atacttgtag ctggtggcgt tgaattgatg agtgccgtac caatgggtgg caacgagccc
    30061 acaaacaatc ctaccttaca atatgatgat ataggtgcgt catatcctat gggcttaact
    30121 gctgaaaatg tagcatccca atttgacgta tcacgcgaag atcaagatgc ttatgctgtc
    30181 agaagtcatc aacgtgccta tgacgcacaa cgtgatggtc ggttcaaaga tgaaattatt
    30241 ccaatacggg taaactcagt tgaatataca aacgcaggac caaaagtaca cacaaatatc
    30301 tttgaccaag atgaatttat acgccctgac accacgatgg aggcattagc caaattacgt
    30361 acagtattta aagctgacgg cactgtgact gcgggaacat ctgccccact ttctgatggt
    30421 gcaggatttg tagttttaat gtctggagat aaagtgaaag aactaggcgt gacacctatt
    30481 gcacgattcg ttggttataa ggcagtaggc gttgacccga aaattatggg tattgggcct
    30541 gcatatgcga ttcctgaagt attgtcactc agcaatctat ctgttgaaga cattgatttg
    30601 atcgaattga acgaagcatt tgcttctcaa acgattgcat ctattaaaga agtaggtcta
    30661 gatatatcac gtacgaatgt gaatggtggc gctattgctt taggtcatcc attaggtgct
    30721 acaggggcaa tgttaaccgc gcgtttactt aatgaaatgg gtagacgtcc cgatagccgt
    30781 tacggcatgg ttacgatgtg tattggtgtc ggcatgggtg cagctgctat atttgagtat
    30841 gtgcgttaga atggttgatt ttggatgaag cggattcgtt aggttattga atgaagtagg
    30901 ctgaagttga agcagattga agttgaagcg ggttgaagca atttcgtttt attaatgaag
    30961 ctgtgtgaaa tatagtgatt gaacaaaaaa gtggtttaat gggatggtgg ttatttccgt
    31021 tttagaattt aacatttaca cgtctaattt taatcattgt tttaaatttt atgaatcgaa
    31081 gccctttgat ttaataatat ttgctaatgc tagtaactta tctgattgtt catgtttaaa
    31141 ataaagaaaa ccactcacat cagtgtgtgt tcgaactaga cttgtaagtt ccagttcggt
    31201 acgactttct aaagcaatta ttattgctgt gattgtcgta tgtcacatag atgtgcgtgg
    31261 tttattttaa taggttagta atatattagg tcatgttatg tttaagacta taatgaataa
    31321 ataatttaga aatatgcttc cgattgttcg atgctttaat tcagttagaa gcatcataga
    31381 atgcatgatt actgttgtaa agatacataa tgttttgtat tgactgtatg tctatggata
    31441 gagttataaa cttattttgt tactctagga ccatatgtcg cagtaccatc tgcatgtgtt
    31501 gttacgttat atgcgtttgt tttacttggc ttgttttgtg tcgggcgagc tccgtatgat
    31561 acttggccgt ttgcatgtgt tgttacgtta tatgcatttg ttttgcttgg cttgttttgt
    31621 gtcgggcgag ctccgtatga tacttggccg tttgcatgtg ttgttacgtt atatgcattt
    31681 gttttgcttg gcttcttgta tgtcggacga gctccgtatg atacttggcc atttgcatgt
    31741 gttgttacgt tatatgcgtt tgtttcactt ggcttgtttt gtgttgggcg agctccgtat
    31801 gatacttgac catttgcatg tgttgttacg ttatatgcgt ttgtttcact tggcttgttt
    31861 tgtgttgggc gagctccgta tgatactgtg ccatcttgat ttgtcgttac gttgtatgca
    31921 tttgtttcac ttggcttgtt gaatcttggt ctcgcttcat atccaaatgt tccatcgttg
    31981 tattcacgga tacctgtacc agcatctcta tatttcacat acttaggtgt tttgttaaat
    32041 tgcggtctcg gaccatattg agaagcttct gttgtttcag ttgcttgagg tttaacttca
    32101 atatcacttg attctccttg aatacctttc aacgttgatt cagtaccttg tggttttatt
    32161 tcaagtttag atgagctacc ttcaagacct tctaaaatag ggttcggtgt cgtcggttga
    32221 gtataattat cgcttaaaga tggtctgttt tgttccattg ttagaaaatc gggaccttga
    32281 acgatttcac cttgtaacgt tttattttcc atcgttggat attccggacc ttttacaatt
    32341 tcacctgtaa ttgtgccctg tggaatttta actaatggct gtgccactgg ttgtgttgtt
    32401 tcttcagctt tatcaactgt aattttaacc tcttgttggt tatcaaattt aggtgattga
    32461 ggttcttcaa ctttcttctc ttcttttaca actggagatt ttgttacagt ttcctcgtat
    32521 tttttgacag ttttattttt ccaagattcg tcagcctttg caaccgcttc tcttgtttct
    32581 ttaactagag catcaaatcc atctttatgt ttatggtaat catgtttagt tccatcatac
    32641 ctagtaatgt gtttaggtct attcaacttg gtttcaatga agaaatcatc aataatagat
    32701 tctaaatctt taatcatttc tttttcagta cgttgattcg taactctaat tggatctttt
    32761 tcatcaccta aaatcaaatc aattttcgcc ctaagattta atgcttctgt tttatgttgt
    32821 gcatctgtaa cgtaggcaaa atatacacta tatgcttgat caacaagtga atctatttta
    32881 ccatatgctg ttctttcttc gctttcagaa tatggtttta aatctggatt ctctgattca
    32941 attttcttta cttctaactt aaagttatcc aatgcttttg ttaataattt tgataagtca
    33001 ttgtattctt ccatagttaa atcgccaaga tcgtatttat caaatgcttt cattttaact
    33061 tgagtattag gattctcttt tttgtatttt ttatatagtt ctttataaat ttcgtatttt
    33121 tctttttttc tttctagcag gtattggcct tcctctaaaa ctctagtttt taatttatct
    33181 actgcatctt tatattcttt ttctgcatat ttatgctgat ccagtaaatg aaaaatattg
    33241 taaaactggt tttctagatt ttcaattatt ccccaataat atccatctgc aatttgtttc
    33301 ccatttttac tcccagcatt aacttgtgat ttcccactat aatcctttgt tactatcgca
    33361 tctgctttgt tatcccatgt aaataagcta gatgcaactg ctaatgcgcc tagcgaaatt
    33421 atttgctttt tcataatttt ttaattcctc caaaatgtaa ttgcccaatc tacattaaag
    33481 aaacaaaata ttaaaagaca ttaactatat attaactaga ataaacaaag cattaactat
    33541 ctttttgtta tagttaatta gctttgcaat atacaatagt gtataatttt tttgattaat
    33601 gtacatcaaa ggagtaacaa agcatgacaa cacaaatgaa aatcaaaaca tatttagttg
    33661 ctggtattaa agcggcgctc cttgatacga ctggtattaa attagcaagc aaatctgaaa
    33721 ctacatcaca tacgtatcaa catcaagcgc ttgtagatca attacatgaa ttaatagcaa
    33781 acactgactt aaataaatta tcgtacctaa atttagatgc gtatcaaaaa cgcgatattt
    33841 tagctgcaca ttatattgca aaatccgcta tacgcactaa aaatttggat caaatgacta
    33901 aagcgaaaca tagattagaa agtatttacg attcaatttc taaccctttg cattcacaaa
    33961 acaattagca acttatcaat aaatcatgta agtgtttgtg acgccaaatt gccatacaaa
    34021 tacttgtcat atgaatataa acgaatgaaa cgattgccta tcccatagat ggcaacatta
    34081 aaaagacctc tgaaggtatc atcttgataa ctttagaggt ctttgtttat attattcaaa
    34141 caaaattctt atagaacgat cgaagtatgt ttcgtctttc ttttcttctt taatctgatc
    34201 agctaatgct gggtcatctg tgataatacc atcaacattg gtttgtaagt atttcgttaa
    34261 atcttcttcg ccgttaatag tccaagtata gacttcttta ttttccaagt gcgcttgatt
    34321 aacaagtctt ggcgaataag aaaaatcttc gatgacaaag aaatctaatg atgtttcttt
    34381 aaaatgacca aactgcaacg gaatgatata accacacttg agatatggcg cttctttttt
    34441 caacttagtc atcacatcat aatccaatga catcacacga tattgatgtt caacaccatg
    34501 ctttttcaaa atatcaataa cacgttgtgt ataatctgct ggttctttac catgtggctt
    34561 taactctact agtagcttca catttgattg tttagccgtt tcaataaatt cgtctaagga
    34621 tacaagtttt gcttcatgtc cattttgacg cattttcaaa ccgacgacat ctttgaaatt
    34681 agattcagaa atgtttttat taacacctgt taaacgtttc aaattgttat catgactaac
    34741 aacaaattgt ttatctttcg tcataattgt atctaactca acgtattcga cattcgcttt
    34801 tgcagcagct ttcaatgacg gaatagaatt ttcaacgcct ttatcttcga aaccacgatg
    34861 gccaataata gaaatatttg tattgatagt attattgtaa agtaagtaca tgttataacc
    34921 gataaaacat gtcactgcaa gcaccattga aattataaag aacctagact tccgtttcgg
    34981 ttttgggtat ttaaattcta agcccggttg gtctaataca ttctcttgtt ttaagtgcag
    35041 tactaacaca ctgattaatg ataatttcgt aaataaataa tagaagaaca atgcgctttt
    35101 caatacaaca aataaaattg atgagactaa aaacttatct ccttcttcat ctacacaaat
    35161 agcaagatat gttgctcctg aaataattaa tgttaaaatc gcaccaatga tgagttccaa
    35221 tataactatt tctataacaa gtcgaaactt atttcgcttc gtaatttgcc aacttagtct
    35281 catattttta aataacgact ggcggtttaa aa
//
LOCUS       DAHHYJ010000011        34300 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000011 DAHHYJ010000000
VERSION     DAHHYJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34300
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.011"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            335..1045
                     /gene="deoD"
                     /locus_tag="NBK22_000406"
     CDS             335..1045
                     /gene="deoD"
                     /locus_tag="NBK22_000406"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD2413621.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNDAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            complement(1166..1609)
                     /locus_tag="NBK22_000407"
     CDS             complement(1166..1609)
                     /locus_tag="NBK22_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCD2413622.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLENAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(1809..2222)
                     /locus_tag="NBK22_000408"
     CDS             complement(1809..2222)
                     /locus_tag="NBK22_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCD2413623.1"
                     /translation="MPIAYYDGNCIYCYNYVIWLIQHDLPRNYQFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIKLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            complement(2680..4053)
                     /locus_tag="NBK22_000409"
     CDS             complement(2680..4053)
                     /locus_tag="NBK22_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCD2413624.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRISNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            complement(4725..4955)
                     /locus_tag="NBK22_000410"
     CDS             complement(4725..4955)
                     /locus_tag="NBK22_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413625.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            complement(4986..5924)
                     /locus_tag="NBK22_000411"
     CDS             complement(4986..5924)
                     /locus_tag="NBK22_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCD2413626.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            complement(6360..7025)
                     /locus_tag="NBK22_000412"
     CDS             complement(6360..7025)
                     /locus_tag="NBK22_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2413627.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNHQEFQIISGEQDIKDALTQFEI
                     EKD"
     gene            7177..7497
                     /gene="czrA"
                     /locus_tag="NBK22_000413"
     CDS             7177..7497
                     /gene="czrA"
                     /locus_tag="NBK22_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCD2413628.1"
                     /translation="MSEQYSEINTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            7499..8479
                     /gene="czrB"
                     /locus_tag="NBK22_000414"
     CDS             7499..8479
                     /gene="czrB"
                     /locus_tag="NBK22_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCD2413629.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDYHVWTISNDMNALSCHVVADHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            8745..9836
                     /locus_tag="NBK22_000415"
     CDS             8745..9836
                     /locus_tag="NBK22_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2413630.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPVDAYLKVIAVLLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKNKKVVTEPKNTKVENDKVTTSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(10278..10382)
                     /locus_tag="NBK22_000416"
     CDS             complement(10278..10382)
                     /locus_tag="NBK22_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413631.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISTQDHKK"
     gene            11062..11220
                     /locus_tag="NBK22_000417"
     CDS             11062..11220
                     /locus_tag="NBK22_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCD2413632.1"
                     /translation="MSKSSDALFNSLTQSFELIEGVYNELVTDNLKIVMYKPRKESTS
                     GRINRRFK"
     gene            complement(11878..12735)
                     /locus_tag="NBK22_000418"
     CDS             complement(11878..12735)
                     /locus_tag="NBK22_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2413633.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITTFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     EHFGIHQDETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(12803..13585)
                     /locus_tag="NBK22_000419"
     CDS             complement(12803..13585)
                     /locus_tag="NBK22_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2413634.1"
                     /translation="MLIQLDQIGRMKQGKTILKNISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATTGGVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERVIDVVI
                     SGAFKSIGVYQDIDEEVRNEAHQLLKLVGMSAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLSILDSLSDSYPMLAMIYVTHFIEEITSNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            complement(13817..15622)
                     /gene="glmS"
                     /locus_tag="NBK22_000420"
     CDS             complement(13817..15622)
                     /gene="glmS"
                     /locus_tag="NBK22_000420"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCD2413635.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     EGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            16303..17841
                     /locus_tag="NBK22_000421"
     CDS             16303..17841
                     /locus_tag="NBK22_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCD2413636.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPLM
                     KFIMHILSVAVEALVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VAALIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            17876..20008
                     /locus_tag="NBK22_000422"
     CDS             17876..20008
                     /locus_tag="NBK22_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD2413637.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VEAYLTSFSLTLERANKKGLRIAGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTKSSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDSSYLTVNPLLPDSDISYVAQFLNTKSTFQEMHDKSSNMI
                     DKDDVHVETKDIDGNTSFENEQTSNLTSVFEKHLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRSKFLERIKKQFI"
     gene            20020..20454
                     /locus_tag="NBK22_000423"
     CDS             20020..20454
                     /locus_tag="NBK22_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2413638.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            20454..21560
                     /locus_tag="NBK22_000424"
     CDS             20454..21560
                     /locus_tag="NBK22_000424"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD2413639.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     PFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTADQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(21879..29309)
                     /locus_tag="NBK22_000425"
     CDS             complement(21879..29309)
                     /locus_tag="NBK22_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HCD2413640.1"
                     /translation="MNLFRQQKFSIRKFNVGIFSALIATVTFISTNPTTASAAEQNQP
                     AQNQPAQPADANTQPNANAGAQANPTAQPAAPANQGQPAVQPANQGGQANPAGGAAQP
                     NTQPAGQGNQADPNNAAQAQPGNQATPANQAGQGNNQATPNNNATPANQTQPANAPAA
                     AQPAAPVAANAQTQDPNASNTGEGSINTTLTFDDPAISTDENRQDPTVTVTDKVNGYS
                     LINNGKIGFVNSELRRSDMFDKNNPQNYQAKGNVAALGRVNANDSTDHGNFNGISKTV
                     NVKPDSELIINFTTMQTNSKQGATNLVIKDAKKNTELATVNVAKTGTAHLFKVPTDAD
                     RLDLQFIPDNTAVADASRITTNKDGYKYYSFIDNVGLFSGSHLYVKNRDLAPKATNNK
                     EYTINTEIGNNGNFGASLKADQFKYEVTLPQGVTYVNDSLTTTFPNGNEDSTVLKNMT
                     VNYDQNANKVTFTSQGVTTARGTHTKEVLFPDKSLKLSYKVNVANIDTPKNIDFNEKL
                     TYRTASDVVINNAQPEVTLTADPFSVAVEMNKDALQQQVNSQVDNSHYTTASIAEYNK
                     LKQQADTILNEDANHVETANRASQADIDGLVTKLQAALIDNQAAIAELDAKAQEKVTA
                     AQQSKKVTQDEVAALVTKINNDKNNAIAEINKQTTSQGVTTEKDNGIAVLEQDVITPT
                     VKPQAKQDIIQAVTTRKQQIKKSNASLQDEKDVANDKIGKIETKAIKDIDAATTNAQV
                     EAIKTKAINDINQTTPATTAKAAALEEFDEVVQAQIDQAPLNPDTTNEEVAEAIERIN
                     AAKVSGVKAIEATTTAQDLERVKNEEISKIENITDSTQTKMDAYNEVKQAATARKAQN
                     ATVSNATNEEVAEADAAVDAAQKQGLHDIQVVKSKQEVADTKSKVIDKINAIQTQAKV
                     KPAADTEVENAYNTRKQEIQNSNASTTEEKQAAYTELDTKKQEARTNLDAANTNSDVT
                     TAKDNGIAAINQVQAATTKKSDAKAEIAQKASERKTAIEAMNDSTTEEQQAAKDKVDQ
                     AVVTANADIDNATANTDVDNAKTTNEATIAAITPDANVKPQAKQAIADKVQAQETAID
                     ANNGSTTEEKTAAKQQVQTEKTTADAAIDAAHTNAEVEAAKNAEIAKIEAIQPATTTK
                     DDAKQAIATKANERKTAIAQTQDITAEEIAAANVNVDNAVTEANSNIEAANSQNDVDQ
                     AKTTGENSIDQVTPTVNKKATARNEITAILNNKLQEIQATPDATDEEKQAADAEANTE
                     NGKANQAISAATTNAQVDEAKANAEAAINAVTPKVVKKKAAKDEIDQLQATQTNVINN
                     DQNATTEEKEAAIQQLATAVTDAKNNITAATDDNGVDQAKDAGKNSIQSTQPATAVKS
                     NAKNDVDQAVTTQNQAIDNTTGATTEEKNAAKDLVLKAKEKAYQDILNAQTTNDVTQI
                     KDQAVADIQGITTDTTIKDVAKDELATKANEQKALIAQTADATTEEKEQANQQVDAQL
                     TQGNQNIENAQSIDDVNTAKDNAIQAIDPIQASTDVKTNARAELLTEMQNKITEILNN
                     NETTNEEKGNDIGPVRAAYEEGLNNINAATTTGDVTTAKDTAVQKVQQLHANPVKKPA
                     GKKELDQAAADKKTQIEQTPNASQQEINDAKQEVDTELNQAKTNVDQSSTNEYVDNAV
                     KEGKAKINAVKTFSEYKKDALAKIEDAYNAKVNEADNSNASTSSEIAEAKQKLAELKQ
                     TADQNVNQATSKDDIEVQIHNDLDNINDYTIPTGKKESATTDLYAYADQKKNNISAGT
                     NATQDEKQQAIKQVDQNVQTALESINNGVDNGDVDDALTQGKAAIDAIQVDATVKPKA
                     NQAIEVKAEDTKESIDQSDQLTAEEKTEALAMIKQITDQAKQGITDATTTAEVEKAKA
                     QGLEAFDNIQIDSTEKQKAIEELETALDQIEAGVNVDADATTEEKEAFTNALEDILSK
                     ATEDISDQTTNAEIATVKNSALEQLKAQRINPEVKKNALEAIREVVNKQIEIIKNADA
                     SAKEIARTDLGRYFDRFADKLDKTQTNAEVAELQNVTIPAIEAIVPQNDPDANDTNNG
                     IDNNDATANSNANATPENTGQPNVSETTDNGKADASPTTPNNSDAATGETTATSATDD
                     ANDKPQANNNSSVDASTNSPTMDNDVTSKPEVESTNNGTTDKPVTETDNATPAESTTN
                     NNSTTTATNENAPTGSTATAPTTASTEAASSADSKDNASVNDSKQNAEVNNSAESQST
                     NGKVAQPKSENKAKAEKDGRDSTNQSMVESTTETLPSADITEPNVPSNTSKDKEESTS
                     NQTDAGQLKSETKVASNEADKSPSKADTEVSNKPSTSASSEAKDKMTSTNVSQKDDTA
                     TADTNDTQTSVGPVANNKATQNDGANASPATVSNGSNSANQDMLNVTKPEENKANAKS
                     AQQGKVNKAKQQAKTLPDTGMSHNDDLPYAELALGAGMAFLIRRFTKKDQQTEE"
     gene            complement(29587..30942)
                     /locus_tag="NBK22_000426"
     CDS             complement(29587..30942)
                     /locus_tag="NBK22_000426"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCD2413641.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            complement(30969..31901)
                     /locus_tag="NBK22_000427"
     CDS             complement(30969..31901)
                     /locus_tag="NBK22_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCD2413642.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            complement(31903..32712)
                     /locus_tag="NBK22_000428"
     CDS             complement(31903..32712)
                     /locus_tag="NBK22_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCD2413643.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            complement(32901..33809)
                     /gene="rocF"
                     /locus_tag="NBK22_000429"
     CDS             complement(32901..33809)
                     /gene="rocF"
                     /locus_tag="NBK22_000429"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCD2413644.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLISRLKQLDL
                     DVYDKGDIKVPAVNIEKFHSEQKGLRNYDEIIDVNQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     LNSNVIKPENIVLIGMRDLDKGERQFIKDHNIKTFTMSDIDKLGIKEVIENTIEYLKS
                     RNVDGVHLSLDVDALDPLETPGTGTRVLGGLSYRESHFALELLHQSHLISSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            complement(33947..34019)
                     /locus_tag="NBK22_000430"
     tRNA            complement(33947..34019)
                     /locus_tag="NBK22_000430"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33984..33986),aa:Lys,seq:ttt)
     gene            complement(34023..34097)
                     /locus_tag="NBK22_000431"
     tRNA            complement(34023..34097)
                     /locus_tag="NBK22_000431"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34063..34065),aa:Gln,seq:ttg)
     gene            complement(34107..34190)
                     /locus_tag="NBK22_000432"
     tRNA            complement(34107..34190)
                     /locus_tag="NBK22_000432"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34154..34156),aa:Tyr,seq:gta)
     gene            complement(34220..34295)
                     /locus_tag="NBK22_000433"
     tRNA            complement(34220..34295)
                     /locus_tag="NBK22_000433"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34260..34262),aa:Val,seq:tac)
BASE COUNT        10375 a         6100 c         5237 g        12588 t
ORIGIN      
        1 atcaattttg cactattcat tttctatctc ctcctttatg gttgactata aaaatacggt
       61 tgtaaattag ttgtttgagc gtcaggttca tttaaatatc aggttagatg ttcgcttttt
      121 atgtaaccgc atacatatac tattacatta attcatttcc cataaacaaa caatacaatt
      181 gaacgtgata tcttcattat gaacgatgac ttgacaacaa gctaatcagg attatatttt
      241 tataattctt taattctata gtacaaaaat tcgcaaaaaa gggaaacaaa tgttatctta
      301 aaattattaa tgaatattaa ggagaagata acaaatgaca aaaggtacac cacatattca
      361 accaaatgga gtaaaaattg ctaaaacagt attaatgcct ggcgatccgc tacgtgcaaa
      421 atatattgct gataattttt tagaaaatgt tgaacaattt aacgatgtac gtaacatgtt
      481 tggttacact ggtacatata aaggtaaaga agtttctgta atgggttctg gtatgggtat
      541 tccaagtatt ggtatttact catatgagtt atacaacttc tttgatgtag atacaatcat
      601 tcgtatcggt tcttgtggcg cattacaaga aaatgttaac ttatacgatg ttattattgc
      661 acaagctgca tcaactaatt caaattatgt agatcaatac aatattccag gtcatttcgc
      721 gcctatcgct gacttcgagt tagtaactaa agctaaaaat gtcgctgacc aaatcggtgc
      781 tactacacac gtaggtaacg tattatcttc tgatacattt tacaatgccg atccaacatt
      841 caatgatgct tggaaaaaaa tgggtatttt aggtatcgaa atggaatcag ctggtttata
      901 tttaaatgcg attcatgctg gtaaaaaagc acttggtatt ttcacagtaa gtgatcatat
      961 tttacgtgac gaagctacta cacctgaaga acgtcaaaat tcatttacac aaatgatgga
     1021 aatcgcttta gaaatcgcag agtaacttat ttaaattgac tttaattgct ctttaacaat
     1081 gcgattaaac tcaaaaagcc aacacattct gggcgtatcc ccatttatgt gttggctttt
     1141 atttatatta ttacttatct gtagattagc ttaagtaaga tttaaacatc caattatgtt
     1201 tatctactga tgtttgcata cctataaaca tatcttctga tacatcatcg ccagcattac
     1261 cagcaatttc gattgcgttt tctaattgtt ttgagatatt tgtgaagtct tgtgataatt
     1321 cttcaaccat ttgttctgca gagtaacctt tcgcagcttc tttaacaatt gattgctcta
     1381 agcattcagt taatgtacct acagggtttc ctcctaccgc taaaattctt tcagctaatt
     1441 cgtctacata ttggcttgct tcattatata attcttcaaa tttaacgtgt aatgagaaga
     1501 agttaggccc tttcacatac cagtggaaat tgtgtagctt tgtgtaagct actgtccagt
     1561 ttgctacttg ttgattcaat tcttttacaa catcttgttg attactcata tttaatacac
     1621 tccttaaaat tgtctacgtc ttgcttattt atattaatta ttatattata atgattctaa
     1681 tctgtaaagt gtttttacct tcttttttat aattattcta atctaaatgt aaagtgttcc
     1741 gctttttaaa tgcctaaatt ttaattcatc atctaatata tccattatca attagcaagt
     1801 atgttttatc aatcagtaag ttttactttc gcattatcat ctaaaaagaa cgatttttca
     1861 taatcatttg gttggtgcca tgttgttttc cacattctat ttctattatt agcaaacatt
     1921 cgataaccga aattacggac tggtttaggt acaagccata aacctacgcc caataattta
     1981 gttcgattag gtagtgctgt gattagttta ataatcgcct gcgattcaaa atatatacga
     2041 tcacctttct gtaaaatcac actattttta tttgccgctt ctggatgttg tttaaaaaat
     2101 tgttggccaa cttcaccttt taacgtggca aattgataat ttcttggtag gtcatgttga
     2161 attaaccaaa tgacatagtt ataacaatat atacagtttc catcataata tgcgattggc
     2221 ataatttatt cgctccttta atagtactta tagtcatttt tcctaattta aaaacattta
     2281 aacaaggttg tcacttgata agtcctcgcg acacgaaatt gccataaaat ttatttttca
     2341 gttttatatt tattgtataa tgtatttttt atttacttta aatttctcaa tctacagtgt
     2401 aatcttattt cttattatta acttagcgtt tggtcattcg tcttattcaa cataccactg
     2461 gaaaactcat ataaagtttt aattctgtaa cataaattct tttatattca tatactgtct
     2521 ttattattta ttaaaataaa aacgagtaat ctatcataag ctcttaatta gctcttatgg
     2581 ttgattaccc gtcatttttt ctttatgcgg ttattcaaat atgcaattat gcaattttta
     2641 atcctatcct tacttctttt caaaaactac catatatcat tagtcatgtt tgactttgac
     2701 tatacgtgtg tattttaaaa actgctctga atatttctcc ttaatttcct ctaaactgtc
     2761 atatgtaata cctacaatat gccaattagg attgaaataa tagaaagaat ctttttgtcg
     2821 tgaccattta atcatattac cgtctttatt ataacgtggt aatgttactt catagccttc
     2881 taacacatta atatacgttt ggaaaatgtc tgtatctata cgattaaaat tatcaataac
     2941 taaataactt ttatcctttt taggcattaa ttgagtaata aacccttcac gatagtataa
     3001 tgcccctgtt tcatttggta aatacttgcc ataaagcata tcttctgaaa aatcaggatg
     3061 acctgttgtt ataacaggat ttgcattcgc tttttgcaat aagtcattag ccgcttttaa
     3121 gccttcttct tttcgatcta caacaagtaa aataaacggc tttaacgctt cttctttttc
     3181 actttcaagc atatctggtt tttgaaccat ttcaaatgga gatttcaatc cattattatc
     3241 gctcatttca ataattgcat catactgtgc ttgtgacata cttgcaatag cctgttttgc
     3301 attttcttga aggaaatata agtttttcaa tttaggatgc ttatttaatg tacttaatgt
     3361 aatcggtgta atgtctttct cataagacac ttcaatcact gtactatttg ttctaccagg
     3421 aattggtggt ttttcatgaa tatgctttga tacttctcca attccaacga cagattgatt
     3481 tttcgttcga ttataaaaaa taatattgtc gccttcttct aactgagtat aaaaatgata
     3541 accattacgt ttaataccgt tgtacgtgtg cgtataaatc gtatattggt ttccaggttc
     3601 aaattcttca gtttcagcta aaaagaaata acgcggtatc ttaatttcgc ctttaccaag
     3661 accacttatt aaatcaaact cttctgcagt gatttgattg aacaatgtct ctttcatatt
     3721 acttatacga aattccaaag cttcactacg ctttaaataa tctgctgtta atggtttcaa
     3781 ttgttcatta aaacgaaact gtacacgtat tttattttgt gcacctgttt caacactaat
     3841 aatttcacca catccaagta gtccagtatc cgtctgaact tgataaaaga tgacttgatc
     3901 tcctacttta gcctttttaa acgctctaaa tccttgagat gggttaaaat gtgcgcctga
     3961 ttcaaataaa gctgtttgtc ctactaacgg ttcattatga ttccaacggt tatatccaca
     4021 attcaaccaa aaataattcg tttctgctgt catcttaata ctccttaacc tgaataaatt
     4081 ttagaaacac tatgaattac attcttttag tgtttcttat gcagttggac gcgtatgcga
     4141 acaactgtat accctttgtt cactgcgatt ttaatcgcat ttcctataac attgtagcgc
     4201 ccaggacatt aatttacgtc ccagaccctt atcgttttca cttctaagta agtcgaacta
     4261 ttttgcttta caacaagtgc gactctaaat acagttggac acacatacga gcaactgtat
     4321 accttttaat cagttttcta tattttattt attatatctg tcttaatgat aaaaattgtt
     4381 acaaacagtt taacatattt agctacctat tttgcatata aaaaacatgc ttgcatacac
     4441 tatgcaataa aaattacaaa tttatatatg ataccactat gcttgcttat ctctatagcg
     4501 ccattgatac acatttttaa atatctatac tgccgttaga attttatcat gtcttaattt
     4561 tcattaaata ttaattactt cattttatat aaaccaacaa aaaccccctc actactatgc
     4621 aagtgagagg ttatgttgat gtgctttatt ttcatcaatg tcattaacac aatcatattt
     4681 cttctgttaa tgcactgtat tatattcaaa cgcgctatcg acaattaatt tgaagctaca
     4741 aaggtaccgc ataatggcag cacattaatt aacaatgcgc taaactttaa tgaggcgctg
     4801 atttgcttgt ctttaaaagt caacatagca ctgataattc ctaaaatagc tactatacgt
     4861 ggaattgtca tgctcggata ctgcggtata tgtaagaaag caccaagacc tgctatcaca
     4921 ccacatacta acgcaatata tagaaattgc ttcaaatcat tcactcctaa attgttatta
     4981 cactattaca catagctaat catcaatgtg aaatcacctt caaagacact atccaaatct
     5041 tcagaagtca aaataaagtt tgtaccagta gtcagtttga aaatttcacc atcgacaatc
     5101 atttgccctt cgccttccaa cactgtaact aaacagaact ctctaggctt catataattt
     5161 aacgtgccag aaatttccca tttaaccaat gtaaagaaat cattcgatac aatgtgtgta
     5221 cacttatggt tttcaataat ttcgctttca ggcaaaatat taggtaatgg tgcattgtac
     5281 tgaataacgt ctaaagcttt ttcaatattt aacggtctat cattatattg attatcttga
     5341 cgattgaaat cataaagtct atatgtaatg tctgacgatt gcatcgtttc gtatgctaat
     5401 attcctgaag aaatagtatg tactgttcct gctggaataa aatagaattc tccaggtttt
     5461 actttaatat atctaagtat cgactctatc gttccgtgtt gaacatgatt cgcaacttct
     5521 tctctagact ctgctaatgt ccctataact atttctgcat cttcttctgc atctataata
     5581 taccaacatt cagatttgcc atattgcccg ttttcatgct cataagcata agaattatca
     5641 gggtgcacat gaatagaaag tgattctctt gcatccacta ttttagttag aagcggaaaa
     5701 tctttgcttg ggaaatcacc aaacaattca cgatgttctg accaaatacg gtctaatgtt
     5761 tgaccttgat atggtccatt aataatctcg ctcgtaccat ttggatgtgc tgacacacac
     5821 caacattccc cagttgtatc attgtctaat tgatatccaa actcacttag acgttgaccg
     5881 ccccataatt ttgtttttaa aattggttgt aaaaataatg gcattgttgc acctccattg
     5941 tgattaagta agcaatagaa ctctgatgtt gttgttccat tatattttga ttttgttctc
     6001 atttacatcg tattataaac ttccacattt caaattaact attagtgatt gtaccatatt
     6061 tactaacatt gcagtactgc caattaaagc gcttcactta aatttacagt actttaacat
     6121 ttttaaaaat taatagcata gagattatct ctctcttaca tttgtacaca tttcccttta
     6181 aatttactcg cccattatac caattaataa acaactttaa tagttgtgcc atacattgtt
     6241 caaattcttt gtaaaatgca tagacaatac gtacttattc atacttataa ttcatcattt
     6301 tcaaaaaaat aacgagttac gaaaacgtaa cccgcttcaa atcatattta ctatccttat
     6361 taatcctttt caatttcaaa ttgagttaaa gcatctttaa tgtcctgctc accactaata
     6421 atttgaaact cttggtgatt aaaatgattg gatgtgacaa tttcttttaa tactgtcgca
     6481 acatcttctc taggaatttc acctttacca tcaaaatatt gtgcagcttc tatctttcca
     6541 gatcctgctg catttgtaag tgcccctgga tgtaaaattg tataattcaa acctgaacgt
     6601 cttaaatagt catcagcgta atgtttagct attgtatatg gctttaaatc accgctatca
     6661 tcaaaagcct gacgtctcga atcatatgtt gaaaccatga catagtgttt aatattagcc
     6721 tctttactcg caatcattga tttaacagca ccatctaaat cgacaataat tgttttatct
     6781 gcacccgtgt tccctccaga acctactgaa aagataactt tatcgaatgg tttaaacgtc
     6841 tcagttaaag tctctattga atcattttca acatcaacaa gaattgcttt cataccttgt
     6901 gattttaacg cattaagttg atctgattgc ctaacaccag cagtaaatgg tacattttct
     6961 tttgctaatt gttgcactag taacgaacct acaccgccat tagcacctat aaccaaaata
     7021 ttcatttaca acactctcct atttattatt ctctatgcca taccacttta tgagatatgt
     7081 aaaacttgtt acaactataa aaatcaattg acatactact gggaacgtat taaattaata
     7141 tatgaacaaa tattcatatg aaaggatttt catataatgt cagaacaata ttcagaaata
     7201 aatacagata cattagaacg cgtaactgaa attttcaagg cattaggcga ttacaatcga
     7261 atacgtatca tggaattgtt atcagtcagt gaagcaagtg ttggtcacat ttcacatcaa
     7321 ttgaatttat ctcaatcaaa tgtctcgcac caattaaaat tacttaaaag tgtgcatctt
     7381 gtgaaagcaa aacgacaagg ccaatcaatg atttattcat tagatgacat ccacgtagca
     7441 actatgttaa agcaagccat acatcacgcg aatcatccta aagaaagtgg gttataatat
     7501 gtctcattca catcatcatc atgaccatat gcatagtcat gtaactacaa ataataagaa
     7561 agtattgttt atatcgtttt taataatcgg tctatatatg tttatcgaaa tcatcggcgg
     7621 tctccttgct aacagcttgg cattactatc tgacggtatc catatgttta gcgacacatt
     7681 ctcattaggt gttgcacttg tcgcatttat ttatgctgaa aagaatgcca caactacaaa
     7741 aacatttggt tataaacgtt tcgaagtact cgcagcgtta tttaacggtg taacgctttt
     7801 tgtaataagt attttgattg tttttgaagc gattaaacgt ttctttgttc cttctgaagt
     7861 tcaatcaaaa gaaatgttaa tcattagtat tatcggttta attgtcaata tcgttgttgc
     7921 attctttatg tttaaaggcg gcgacacttc acacaattta aatatgcgtg gtgcttttct
     7981 acatgttatc ggagacttat taggttcagt tggcgccatt actgcagcta ttttaatttg
     8041 ggcatttgga tggacaatcg ccgatcctat cgcaagtatt ttagtttccg ttattatttt
     8101 aaaaagtgct tggggtatca caaaatcttc aattaacatt ttaatggaag gcacaccaag
     8161 tgatgttgat atagatgaag ttataactac tattaaaaag gattcacgaa tacaaagtgt
     8221 gcatgattac catgtttgga caatttcaaa tgatatgaat gcattgagtt gtcatgttgt
     8281 tgcagaccat acattgacaa tgaaagaatg tgaattatta ttagaaaaca ttgagcatga
     8341 tttattacat ttaaatattc accatatgac tattcaatta gaaacgccta atcacaaaca
     8401 tgatgaatcg attatatgtt caggaacaca tagtcattca cataaccatc atgctcatca
     8461 tcacgcgcat gtacattaat aattttaacc tactgccatt gcatcgatta aacttttcaa
     8521 tggcagtagg ttttttatgt ctttatggcg acttgtttgg tctttgatga tgcaatgttt
     8581 attaacaaat tttcaactat tatttcttac attagtcata tttttgacaa tttactatta
     8641 taattctcta actttagtca ctttaattaa tttttattag atattaatat gaaaataacg
     8701 tgttttttgt tattgtattt atatgaagaa aaggaggcac cactttgttt acttacttta
     8761 aatcagcatt taaaaatgct aaaccacaat tattaataac attgatatat gcacttatag
     8821 catttgctgt aattgcagtt gtatatttac ttgctaattt ccagcttgct aagtatgtac
     8881 aaactattgc aatttattca caatttgggc aaaaaccacc agtagatgca tatttaaaag
     8941 ttatcgctgt attacttata gcagctgtag tttcactgtt tgttttagtt caaattttta
     9001 tcggaattac aaatgttatg aaacgagcaa tgagtcatga aaaagtaaaa tttaccgatt
     9061 tatttattgc atttaaaaaa ggtaattatt taaagagcgt tctgatcggt cttgtatcta
     9121 tagcaatgat tatcgttctt tcacttctga catcattact atacaaatta ttttctccag
     9181 tatcagaaat gattatgaat tcagtacaat catcatatgc agatagtaca cacctgattg
     9241 gtattgctat tacaactcaa agcatcataa tcattgttgt tttattgatt aaagcaatta
     9301 ttacttggtt attgttaata cctattttca actttatgac tagtttcgtt gaatcaacta
     9361 acgataaagt aaaaacacac ttagctaatg gatttaaagc aatgaaaaat ggtcaaaaaa
     9421 catttttcaa atttttcatt ggaatactat tattaaactt aattattatt ttatttaaaa
     9481 cacctgttgg ttacttgatt tcatttaata cgcaatcact ctctcagagt gttgcagaaa
     9541 atatcataag agtttataca gtaatgacaa ttattttatt cgttgttatt catgcaatca
     9601 ttttaatggg tatcgttcaa tattacttaa aacgtggtca aaaaattact aaagataaag
     9661 taaaaacagc tgacaagaat aaaaaagttg tcactgaacc aaaaaataca aaagtagaaa
     9721 atgataaagt gacaacatct gttgaaacaa aaacagaaaa agcacaagat tcattaaatg
     9781 ataatacaac aaaaacaatg acttcagata aaccagaaga caatcaacca aaataaattt
     9841 tagagcccct tacaacttta gttgtaaggg gctcttatgc agttgatgcg tagcatccaa
     9901 ctgtgttcct ctatttttca actttagttg ttaggggctt ctttctaaaa taactaagtg
     9961 aattatctat atatgaagag gtctccaata ctttttatat aattcgtgat tacattctat
    10021 acttttaatt gtattttctg ctgtatggaa agttggtttt attaattctg attatgttca
    10081 gaactacaat tcataagaaa aatgtgttaa aatatatttg tagtaagtag aagcaaaaga
    10141 tgaaaatctt taactcttga aacacaaaaa gggcaacact cggaaacatg ttaccctaat
    10201 gagcccgtta aaaagacggt gaccttatat tttatttaaa aatagccttc aaaatgccgg
    10261 tcaaagcgaa tagaaggtta ttttttatgg tcttgagtac taatcaatac taaaccaatg
    10321 attgttaata caaccatcag catttcaaaa tcagacatgc tagtcctttc taggagtaac
    10381 aactataacc ataggcatca cccctttatt aagagattag ccaccatcta tccaacttgc
    10441 tcaataaata ttataacatg cacactttta aacagtaatt catagtataa gttttttcat
    10501 ttcttaatca actcgataaa cagttgagtg acataccctt gtctaactag ctatttcttc
    10561 ccctgttatc gtttgttcat catataaaaa tttaatttct cattttttat tctctgataa
    10621 agaagagcac caaccttttc ggctgatatg ctcccttcaa aatagacatt gaaaaatgaa
    10681 aactttgaaa gagaaatcgt cagtctatta ttgataagta ttatcctatc ctcgaaaaat
    10741 tactttcaga cactagtgaa caaaaatttt actataagcg tatactttgg caatattcga
    10801 aggaaaaaca tggattaacc tgtgcatatt atacatttag agcttatata cttaaacatg
    10861 atgaatttac tccttatatt atgaaatgtt atcaatagat gtcgcctaaa gatacaacaa
    10921 gattcgagac aaaacctgat catcaagcac agtttgactg gaaagaaagc attaacttta
    10981 aaacgaaaga taatcaaagg attccactat atataggtgt gctactactt tattattctc
    11041 gttttataat catgcataac aatgagtaaa tcaagtgatg ccttatttaa ttcattaaca
    11101 caatcgtttg agctaataga aggtgtatat aatgaacttg taacggataa cttgaaaata
    11161 gtcatgtata agccaagaaa agaaagtaca agtggtcgaa taaatcgtag atttaaataa
    11221 ttcgctgatg gttttaaatt tcaagtaaaa ccttgtattt ccggcctccc tagaaaaaga
    11281 taaagttgaa tcaataacaa aaatattaga tgaaattaat gcttaccaag acgaattgca
    11341 tttggaagag attcctaagt ttttttaatc ttaatgaacg atttaactac taagttcata
    11401 ctagtacagg taaattgcct attactgcct aagagaaaga aaaaattttc ttacaaccac
    11461 tacagaatgt gaatgtaaaa aactcatata aagtgaaaca tatcttactt atggaatgaa
    11521 atgtttgatt atcccaaaat cacaaatgcg attttggagt tgttagggct ctaacgcaaa
    11581 tacaccccta cttaaaataa cggtagttgt tatagtatac ttaaatgcat aaaccatata
    11641 aaaggtgatg ctaatatgac atacggttca attgtagcta tactctgtgc cctatttgtg
    11701 attttcttca ttccatatat ggagaagaaa gacaaaaaga agaaataaca ctagtatctg
    11761 gattactagc atacaaagga gcagtaagac attttctaat agaaaataac tttactgctc
    11821 ctttgtaaat tactcttcaa ctattgtaaa ttaaataata aataactgcc gatgttatta
    11881 atcagctaaa aaatgctttg ctaaatattt tgctgcgcca tcctcttcat tcgtgtatgc
    11941 cgttacatct gaagttaatg cttggatttc agggcgtgca tttttcatag caactgtata
    12001 atgaccaaat tcaaacattg ctctatcatt gtcgctatct ccaataacta acgtttcatc
    12061 ttgatgaatg ccaaagtgtt caatcatttc tttaatgcct gtacctttat cagtttgata
    12121 agccattgtt tccgcattaa atcttgatga atttgaaaca ctaatctgta gttgcacatg
    12181 attttgcttt aattcatctc taaatgttgt tattttttct aaattagaac tgaatagata
    12241 aatttttgaa tattcacctt caggaaactt agttacccaa tctatcttac cagcaagcgc
    12301 atcttgtctt gaagaccatt cactatgact tacgccatta ataggatctt gactacgaat
    12361 catatctcgc atccatgttt catcttcttt taaagaaact ctattacctt caaaaggaaa
    12421 tacctcataa taaatttgtt ggcgcttagc taaattagta atttgttgca cttgagccaa
    12481 tgataaacca tgcttgaaga taatttctcc atctacttct ccaattgttc catttgaact
    12541 aatgatgcca ttaaccgcaa aatcttgagg tacaagttga tgtatttcag aatgcgaacg
    12601 tcctgttgcc aaaaatactt tatatccttt ctctctcaat tgattaatga catctttcgt
    12661 atacgttgat gcttgattat tttcatgtaa aattgttcca tccatgtcca aaaatattgc
    12721 ttttacgtta tccatattgc cctccactgt ccattttatc ttaattaaat tttgtcatta
    12781 ttacttatta tttgcaaaat atttactcta acattgccat agaaaatcga ttattccatc
    12841 tttgaactgc tacatttttc tggaaaaatc gtgacatgtt ttcagatgtc aaaatatcat
    12901 ctattaatcc ttgttgtaca ctttccccat cttttaatag caaaatttta gtaaagttac
    12961 tagtaatttc ttcaataaag tgcgtaacat aaatcatcgc aagcattgga tatgaatctg
    13021 acaatgagtc aagtatactt aacaacgatt ctcgtgcaat aaagtctaaa ccagctgctg
    13081 gctcatctaa aattaaaacc tggggttgcc ccattaaagc tcgcgcaatc atcactcgtt
    13141 gtttttcacc tgtagataaa taaccaatat attgttgcgc tttagcagac attccaacta
    13201 atttaagtaa ttgatgtgct tcattacgta cttcttcatc aatatcttga taaacaccaa
    13261 ttgatttaaa agcaccgctt atcaccacat cgattactct ttcaccctct tgaaactttt
    13321 ccagtaaact atgagataca aaacctatat gttgtcgtac agtctctgca gaatacccta
    13381 ccttgcctgg cattttacca aatagattaa caccgccagt tgtcgcaggc tcatatgcat
    13441 ttaatatatt taatagcgtt gtcttaccag caccattcaa cccatataat atccatttat
    13501 cacctttagc aatttgccaa gaaatatttt ttaaaattgt ttttccttgc ttcattcgcc
    13561 caatttgatc taattgaatt aacatgcgag gtcacccctt tattgctact ccatacttct
    13621 cataaaatca tattaacata acacccttaa ttgtcagact atttaaataa ataaaacact
    13681 tcctttttac gcatttctgc caaattaaga tgaagtaaaa gctaagtcga cctaaaaaag
    13741 cacccttcta gtcgattaat ctaaaagggt gccatatact ttaattttaa tacatgattg
    13801 attctaaaaa agtgaattat tctacagtaa ctgatttagc aaggttacgt ggtttatcca
    13861 catctaaatc tctgtgtaat gctgcataat atgaaatcaa ttgtaatgca actactgaaa
    13921 ctaatggtgt caataattca tgtacatgag gaatgacata agtgtcgcct tctttttcaa
    13981 gaccctccat agaaataata catggatgtg caccacgtgc tactacttct ttaacgttac
    14041 cacgaattga taaattaact ttctcttgtg ttgctaaacc tacaactggt gtaccttctt
    14101 cgattaaggc aattgtacca tgtttaagtt cgccaccagc aaaaccttct gcttgaatgt
    14161 aagaaatttc tttaagtttt aacgcacctt ctaaacttac gttatagtca atagtacgtc
    14221 cgataaagaa tgcattgcgt gttgtttcta agaaatctgt agcaatttgt tccataattg
    14281 gtgcatcgtc aacaattgct tctattgctg ttgttacttt tgctaattct ctcaataaat
    14341 caatatctgc ttcacgaccg tgctcttttg caacgatttg agacaagatt gataatactg
    14401 caatttgtgc agtatatgct tttgtagatg caactgcgat ttcaggaccc gcgtgtaata
    14461 acaatgtgtg gtctgcttca cgtgataaag ttgaacctgc aacattagtg attgttaatg
    14521 atttatgacc taatttatta gtttcaacta atacggcgcg gctatctgca gtttcacctg
    14581 attgagaaat ataaacgaac aatggttttt cagataataa tggcatgttg tagacaaact
    14641 ctgatgcaac gtgtacttca gttggtacac cagcccattt ttctaaaaat tctttaccta
    14701 ctaaacctgc atggtaactt gtacctgctg caataacgta aatgcgatct gcttctttaa
    14761 catcattgat gatgtcttga tcaattttca agttaccttc agcatcttgg tactcttgaa
    14821 taatacgacg cattactgct ggttgttcat gaatttcttt taacatgtag tgtgcataaa
    14881 cacctttttc agcatctgat gcatcaattt cagcaatata agaatcacgt tctacaacgt
    14941 ttccatctgc atctttaata ataacttcat cttttttaac aataacgatt tcatggtcat
    15001 ggatttcttt atattcgctt gtcacttgta acattgcaag tgcgtctgat gcgataacat
    15061 tgaaaccttc accaacacct aataataatg gtgatttatt tttagcaaca tagattgtgt
    15121 ctttgtcttc agcatctaat aaacctaatg catatgaacc atgtaataat gacacaactt
    15181 ttgtaaatgc ttcttcagtt gaaagtcctt gatttgaaaa gtattcaact aattgaacga
    15241 taacttctgt atctgtttct gaaatgaatg atacaccttg taagtattca ccttttaact
    15301 cttcatagtt ttcaataaca ccgttatgaa ctagagtaaa acggccattt gatgattgat
    15361 gtggatgaga gttttcatga ttcggtacac cgtgtgttgc ccaacgtgtg tgaccgattc
    15421 caacaggtcc atcaaaatcg ctactatcag caactttacg taattctgca atacgacctt
    15481 tttctttaaa tacagttgta ttatcatcat ttactactgc gatacctgca gagtcataac
    15541 ctctgtattc taatttttct aaacctttta ataataattc tttggcatta tcatagccaa
    15601 tataaccaac aattccacac ataacgacat tttcctccat attggaatag tacgcgtaaa
    15661 ttatgattta ttgccgataa tttagattga caatctgctt tcataatata aataggaaca
    15721 tgctatcatc gcattcatcc ataacaaatt aagcatagtt atttttacaa ctatacaaat
    15781 tgctcacact gtactttcca tattaatatt ttttatattc aatttctggc gatcttatta
    15841 actttgtcca ttaagtcacc ctaatgtttt acttaataag ctaacgaatg agccacatcc
    15901 gggatagcat ccgccgatct attcgatcac tatcctcctc gtctacaaat acatatattg
    15961 cactctataa aggccactca tatattaacc tttaatcttc aaatacaaat atttatttgc
    16021 acaggcgctt taactgtact gccgaacttt ccccctttcc attaatcatt attgtacaac
    16081 ggtgttgttt tgttttgcaa atattttcac aataaaattt taaaaatcct aaaacaattt
    16141 ttttgtttta ctttttcaaa atatctatac agtcacattg atgacacttt atttaatttt
    16201 gtcacattta ttttgacaaa gttgattttt gtttatattg agtaacaagt aacgtctcta
    16261 tacactatat atagtcacat atattaaaaa agaggtgtaa atatgtcaca aactgaagag
    16321 aaaaaaggaa ttggtcgtcg tgttcaagca tttggatcat tcttaagtag tatgatcatg
    16381 ccaaacattg gcgctttcat cgcttggggt tttatcgcgg ccatttttat agataatgga
    16441 tggttaccta acaaagattt agctacttta gcaggaccaa tgattactta tttaatccca
    16501 ttattgatcg catttagtgg tggtcgttta atttatgatt tacgtggtgg tatcatcgca
    16561 gcaacagcaa ctatgggggt catcgttgca ttacctgata caccaatgtt acttggtgca
    16621 atgattatgg gtccacttgt tggttggtta atgaagaaga ccgaccaatt gattcaacct
    16681 agaacgccgc aaggttttga aatgttattt aataactttt ctgctggtat tttaggattt
    16741 atcatgacga ttgcaggatt taaaatttta gcaccactta tgaaatttat tatgcatatt
    16801 ttatcagtgg cagttgaagc attagtacat gcacacctac ttccacttgt aagtatttta
    16861 gtagagcctg caaaaattgt atttttaaac aatgcgatta accatggtgt attcacaccg
    16921 cttggtgcag atcaagctgc aaaagctggt caatcaattt tatacacaat tgaatctaac
    16981 cctggaccag gtttaggtat cttacttgct tatatgattt ttggaaaagg cactgcaaaa
    17041 gcaacgtcat atggtgctgg aattatccac ttcttaggtg gtattcatga aatttatttc
    17101 ccatatgtat taatgcgtcc tttattattt atcgctgtta ttttaggtgg aatgactggt
    17161 gttgcaactt atcaagcaac aggatttgga tttaaaagtc ccgcgtcacc aggttcattt
    17221 atagtttact gtttaaatgc acctagaggt gaattcttgc acatgttact cggtgtcttc
    17281 cttgctgcac ttgtatcatt cgttgtagct gctttaatta tgaagttcac tagagaacca
    17341 aagcaggatt tagaagctgc gacagctcaa atggaaaata ctaaagggaa aaaatcaagc
    17401 gttgcttcta agttagtatc ttctgataaa aatgttaaca cagaagaaaa tgctagtggt
    17461 aatgttagtg aaacatcttc atcagatgat gatcctgaag cgctattgga taattacaac
    17521 actgaagatg ttgatgcaca caattacaat aatataaatc atgttatttt tgcctgcgat
    17581 gcgggtatgg gttctagtgc aatgggtgca agcatgttac gtaataaatt taaaaaggcg
    17641 ggcattaatg atattacagt tacaaatact gcgattaatc aattgccaaa agatgctcaa
    17701 ttagttatca ctcagaaaaa actaactgat cgtgctatta aacaaacacc aaatgccatc
    17761 catatttcag tggataattt ccttaattca ccaagatatg aagaactttt aaataatcta
    17821 aaaaaagatg atcaagcata ataattaaat aaattaaaaa atggaggata ccgccatgtt
    17881 attgagtaca cgtgaaaaag aaatgatagc cctattgatt aagtaccacg gtcaatatat
    17941 cactatacac gacattgctc agcaacttgc ggtgtcctct cgtactattc accgtgaatt
    18001 aaaaggtgtt gaagcatatt taacttcatt ttcattaact ttagaacgcg caaacaaaaa
    18061 agggctacgc attgctggcg cagattctga tttaaacgat ttgaagcaat cgattgcaca
    18121 acatcaaacc attgacttat ctgttgaaga gcagaaagta attattatat acgctttgat
    18181 acaagccaag gagccagtta aacaatatag tttagcgcaa gaaattggcg tttctgtcca
    18241 gactttagca aagatgttag atgatttaga gcttgattta aataagtacc aactatcttt
    18301 atctcgaaag cgtggcgaag gcatttactt ggtaggtact gaatcaaaga aacgtgaatt
    18361 tttaagtcaa ttaatggtga ataacttaaa tagtactagc gtttattcag taattgaaaa
    18421 tcattttgtc tttcattcat taaatcaaat ccacaaagac tttgttgact tagagcgtat
    18481 ttttaatgtt gaaagactat taatggacta cctaagtgcc ttaccctacc aacttaccaa
    18541 atcaagttat ttaactttaa ctgtccatat cgtgctctcc atttcacgta taaaaaatgg
    18601 agagtatgtc gcattaaacg atgatattta tgattctgta caaaacacat ttgaacacaa
    18661 agtagcaagc gaacttgctg ataaacttgg tcaaatatat gacgtcacgt ttaatcaggc
    18721 agaaattgct ttcattacta tccatttacg tggagctaaa cgaaaaaatc ttaatgatac
    18781 atcattaaat aatcgttgtg aagaaaacaa aattaaagcg tttgttaaca aagtagaaat
    18841 gatttccggt atgacatttg cagatttgga tactttagta gatggactga cgctacacct
    18901 taatcctgca atcaatcgtt tgcaagctaa tatcgagacc tataatccgt taacagacat
    18961 gattaagttc aaatatccaa gactatttga aaatgtaaga ttagctttaa atgattgttg
    19021 gcctgatttg atttttccag agagtgaaat tgcttttata gttttacact ttggtggctc
    19081 gattaaaaac caaggtaatc gatttttaaa catattagtc gtttgcagca gtggtatggg
    19141 aactagtcgt ctattatcaa ctcgtctaga gcaagttttt agtgagattg agcgtattac
    19201 acaagcatca gtcagcgatt tgaagtcact agatttaagt caatatgatg gcattatttc
    19261 tactgtgaat ttagacatcg actcctccta tttaacggta aacccattat taccagatag
    19321 tgatatcagt tatgtcgcac agtttttaaa tacaaagtct acgttccaag agatgcatga
    19381 taaatcatca aacatgattg ataaggatga tgttcatgtt gaaacgaaag atattgatgg
    19441 caacacatct tttgaaaatg aacaaacttc aaacttaact tcagttttcg agaaacattt
    19501 aagtgacgaa aaatcagaac aattaatgca tcatatgcgt tcgggtttaa ctttgcttga
    19561 ttcagtgaaa atagttagta ccgaagttaa acagtggcaa acatatatcg cagattatct
    19621 atatcaatgc gatgtaataa acgatccaac gtcattcgct gaactactag agcaacgatt
    19681 gattgacaat ccaggatgga tattaagtcc atatcctgtt gcaataccac acctaagaga
    19741 caatatgatt aaacacccta tgattctaat cacagtttta gaagaaccgt taacattgcc
    19801 tagtattcaa aatgacaatc aaacaattaa atatatgatt tccatgttta ttcctgacaa
    19861 tgattttatg gcatcactgg taagtgactt gtccgaattt ttaagtttga aattagaatc
    19921 tattgatact tttatggaaa atccacagga acttgaaaca ttattaagaa gcaaattttt
    19981 agaacgaatt aaaaaacaat ttatttagga gtgtacaaaa tgagcgaatt atttagtaat
    20041 gacaatatct ttttaaatgt aaatgttaac agccaaaatg aagcaattga aaaagcaggt
    20101 aaagccttag ttgatagtgg tgctgtaaca gatgcttata ttcaagcaat gaaagatcgt
    20161 gaacaagtcg tatcaacatt tatgggaaat ggcttagcaa ttcctcatgg cacagatgaa
    20221 gctaaaacaa atgtgattca ctcaggttta acattattac aaatccctga aggcgttgac
    20281 tgggatggcg aagtagttaa agttgtcgtg ggaatcgctg gtaaagatgg cgaacattta
    20341 gacttgttat ctaaaattgc aattacattt agcgaagaag aaaatgtgga tcgtatcgtt
    20401 caagcaaaat ctgcagaaga aattaaacaa gtattcgagg aggcagatgc ataatgaaag
    20461 cagttcactt tggtgctggt aacataggtc gtggtttcat tggttatatt cttgcagaca
    20521 acaatgttaa agtaacattt gcagacgtca atgaagaaat cattaatgct ttagctcatg
    20581 atcatcaata cgatgttatt ttagctgatg agtctaaaac aacgacgcgc gtgaataatg
    20641 ttgatgcaat taattcaatg caaccttctg aagcgttgaa acaagcaatt ctagaagctg
    20701 atattattac aacagctgtt ggtgttaaca tactacctat tattgctaaa tcttttgcgc
    20761 ctttcttaaa agaaaaaaca aaccatgtta atattgttgc ttgtgagaat gctattatgg
    20821 caactgatac attgaaaaaa gcagtacttg atattactgg ccctcttggt aacaatattc
    20881 attttgctaa ctcagcagtt gatagaattg taccattaca aaagaatgaa aatatattag
    20941 acgttatggt tgagccattt tacgaatggg ttgttgaaaa agatgcatgg tatggtccag
    21001 aactaaatca tattaaatat gttgatgatt taacaccata tattgagcgt aaattattaa
    21061 ctgtgaatac aggacatgca tatttagcgt atgctggtaa atttgcaggt aaagctaccg
    21121 tattagacgc tgtaaaagat agttcaattg aagctggctt acgccgtgtt ttagctgaaa
    21181 caagtcaata tattactaat gaatttgatt ttactgaagc ggaacaagct ggttatgttg
    21241 aaaaaataat agaccgtttc aacaattctt atttatctga tgaagtgaca cgtgtcggac
    21301 gaggtacgtt acgtaaaatt ggccctaaag atagaattat aaaaccatta acatatcttt
    21361 ataataaaga tttagaacgc actggtttat taaatacagc tgcattgtta ttgaagtatg
    21421 atgatacagc agaccaagaa actgttgaga aaaataatta cattaaagaa cacggtttaa
    21481 aagcgttttt aagcgaatat gctaaagttg acgatggctt agccgatgaa ataattgaag
    21541 cgtacaattc actttcataa tttattgagc tttgtttgaa acaagaagtt tccaacgtta
    21601 ttcgttaaca atcagtaata atgtagtagt tccgttgaat taacaatatt aaatttctga
    21661 acataaaaaa tactcccttc aacatagaca cttaacttgt gttatgtatg aaaggagtat
    21721 ttttgcgtta ataacttgtt ttattttcga gccacagcca cctattctat ggctattggt
    21781 cattactaaa acaaattcat attaactgtt agacttggtt acttagtaag gaatatttcc
    21841 ctatgaaata actagatgtt cacattcttg aataaatttt attcttcagt ttgttggtct
    21901 ttcttagtga atcttctaat taagaatgcc atacctgcac ctagagctaa ttcagcatat
    21961 ggtaaatcgt cattatgtga cataccagta tctggtaaag ttttagcttg ttgtttagct
    22021 ttattaactt ttccttgttg agctgatttt gcatttgctt tgttttcttc aggcttagtt
    22081 acattaagca tatcttgatt agcactattg cttccatttg aaactgtagc tggagatgca
    22141 ttggcaccgt cgttttgcgt agctttattg tttgcaactg ggccaactga tgtttgcgta
    22201 tcattagtat ctgctgttgc cgtatcatct ttttggctaa cattagttga agtcatttta
    22261 tcttttgctt cagaagatgc agatgttgat ggtttattcg aaacttcagt atcagctttg
    22321 cttggcgatt tatctgcttc gttagatgca actttagttt cagacttaag ttgtcctgca
    22381 tcagtttgat ttgacgtact ttcttcttta tcttttgatg tattagaagg tacatttggt
    22441 tctgttatgt ctgctgaagg caatgtttca gttgttgatt caaccatact ttgatttgtt
    22501 gaatctctac catctttttc tgccttagct ttattttcag attttggttg tgcaaccttg
    22561 ccattagttg attgagattc agcactatta tttacttcag cattttgttt tgaatcattt
    22621 acagatgcat tatctttgct atcagcagat gatgctgctt ctgtgctcgc agtagttgga
    22681 gccgttgctg ttgatcctgt tggtgcattc tcgtttgttg ctgtagttgt actattgtta
    22741 tttgttgtgc tttctgctgg cgttgcatta tcagtttctg ttacaggttt atcagttgtg
    22801 ccgttattag ttgattctac ttctggttta ctagttacat cgttatccat tgtcggactg
    22861 tttgttgatg catctacact agaattgtta ttagcttgcg gtttatcatt tgcatcatca
    22921 gttgctgatg ttgctgttgt ttcacctgtt gccgcatcac tattatttgg tgttgtcgga
    22981 gaagcgtctg ctttgccatt atctgtcgtc tcagatacgt taggttgtcc agtattttct
    23041 ggtgttgcat tagcatttga atttgctgtt gcatcattat tatctatacc attattagta
    23101 tcattagcat ctggatcatt ctgaggcaca atcgcttcaa ttgcaggtat cgttacattt
    23161 tgtaattcag caacttctgc atttgtttgt gttttatcta atttatcagc aaatctgtca
    23221 aaatatctac ctaaatccgt acgtgcaatt tctttcgccg atgcatctgc atttttaatt
    23281 atttctattt gcttgttaac cacttctctg attgcttcca aagcattttt cttaacttca
    23341 ggattaatac gttgtgcttt aagttgttca agcgcactat ttttgacagt agcgatttct
    23401 gcatttgtag tttgatcaga aatatcttca gttgcttttg ataaaatgtc ttctaaagca
    23461 ttcgtaaacg cttctttttc ttcagttgta gcatcagcgt cgacatttac acctgcttca
    23521 atctggtcta gtgcagtttc taattcttcg atagcttttt gtttttctgt tgagtcgatt
    23581 tgaatgttat caaatgcttc aagtccttga gctttcgctt tttcaacttc agcagttgtt
    23641 gttgcatcag taataccttg tttagcttga tctgtaattt gtttaatcat tgctaatgct
    23701 tcagtttttt cttcagcagt taactggtca ctttgatcaa tagattcttt cgtatcttct
    23761 gctttaactt caatagcttg gttcgcttta ggtttaacag tagcatctac ttgaatagca
    23821 tcaattgctg ctttaccttg tgttaatgca tcatcaacgt caccattatc cacaccatta
    23881 ttaatgcttt ctaatgcagt ttgaacattt tggtcaactt gcttaattgc ttgttgcttt
    23941 tcatcttgtg ttgcattagt gccagctgaa atattatttt tcttctgatc tgcataagca
    24001 tataaatctg ttgtagctga ttctttttta cctgttggaa ttgtgtaatc gttaatatta
    24061 tctaagtcat tatgaatttg aacttcaatg tcatctttag aagtagcttg attaacattt
    24121 tgatccgcag tttgttttaa ttcagcaagt ttttgtttcg cttcagcaat ttcacttgaa
    24181 gtcgatgcgt tagagttatc cgcttcgttt actttagcat tatatgcatc ttcaatttta
    24241 gctaaagcat cttttttgta ctcactaaat gttttaactg cattaatttt agcttttcct
    24301 tctttaactg cattatcaac atattcattt gttgatgatt gatcgacatt tgttttcgct
    24361 tgatttaatt cagtatcaac ttcttgtttt gcatcattaa tttcttgttg tgatgcattt
    24421 ggtgtttgtt ctatttgtgt tttcttatca gctgcagctt gatctaattc ttttttacct
    24481 gctggtttct taacaggatt tgcatgaagt tgttgaactt tttgtactgc tgtatcttta
    24541 gcagtagtta catcacctgt agtagttgct gcattaatat tatttaaacc ttcttcatat
    24601 gctgctctaa ctggtccaat atcgttacct ttttcttcat tagtagtctc attattatta
    24661 agtatttcag ttattttatt ttgcatttca gttagcaatt ccgctcttgc attcgtttta
    24721 acatctgttg atgcttgaat tgggtcaatt gcttgaattg cattatcttt tgcagtgttt
    24781 acatcatcga ttgactgtgc attttcaata ttttgattac cttgtgttaa ttgtgcgtct
    24841 acttgttgat ttgcttgttc tttttcttca gtagtcgcat ctgcagtttg tgcaataagc
    24901 gctttttgtt cgtttgcttt tgttgctaat tcatctttcg caacatcttt aattgttgta
    24961 tctgttgtaa taccttgaat atcagcaact gcttgatctt taatttgcgt aacatcatta
    25021 gttgtttgtg catttaagat atcttgatac gctttttctt tagcttttaa aactaaatct
    25081 tttgctgcat ttttctcttc agttgtagca ccagttgtat tatcaattgc ttgattttga
    25141 gttgtcacag cttgatcaac atcattttta gcatttgatt taaccgctgt tgctggttgt
    25201 gtgctttgaa ttgaattctt tccagcgtct ttcgcctgat ctacaccatt atcatcagtt
    25261 gcagctgtaa tattattttt cgcgtctgta actgctgttg ctaattgttg aatagctgct
    25321 tctttttctt ctgttgtagc attctgatca ttattgataa catttgtttg cgttgcttgt
    25381 aattgatcaa tttcatcttt agccgctttt ttcttcacaa cttttggtgt taccgcatta
    25441 atcgctgctt ctgcattagc tttcgcttca tcaacttgtg cgttagtagt tgctgctgaa
    25501 atggcttgat ttgctttacc attttcagta tttgcttctg catcagctgc ttgtttttct
    25561 tcatctgttg catctggtgt agcttgaatc tcttgcaatt tgttatttaa aattgctgtg
    25621 atttcattac gtgcagttgc ttttttatta actgttggtg ttacttgatc aatactattt
    25681 tcacctgtcg ttttcgcttg gtctacatca ttttgactat tagcagcttc aatgttgcta
    25741 tttgcttctg tcacagcatt atctacattc acattagccg ctgcaatttc ttctgctgta
    25801 atgtcttgcg tttgagcaat ggctgtttta cgttcgttcg ctttcgtagc gatggcttgt
    25861 ttcgcatcat ctttcgttgt tgtcgctggc tgaatcgctt caattttagc aatttctgca
    25921 tttttagccg cttcaacttc cgcatttgta tgtgctgcat ctattgctgc atcagcagtc
    25981 gttttttcag tttggacttg ttgttttgca gctgtttttt cttctgttgt tgaaccgtta
    26041 ttagcatcta ttgctgtttc ttgagcttgt actttatctg caattgcttg tttcgcttga
    26101 ggtttaacat ttgcatcagg tgtaatggct gcgattgtag cttcatttgt agtttttgca
    26161 ttatctacat ctgtatttgc tgtagcatta tctatatcag cgtttgcagt aactactgct
    26221 tgatcaactt tgtctttcgc tgcttgttgt tcttcagtag tcgaatcatt cattgcttca
    26281 attgcagttt tgcgttcact tgctttttga gcgatttccg ctttagcatc cgatttctta
    26341 gttgtggccg cttgcacttg attaattgca gcaataccat tgtctttagc tgttgttaca
    26401 tcactgtttg tatttgcagc atcaagattt gttcttgctt cttgcttttt agtatctaat
    26461 tctgtatatg cagcttgttt ttcttctgta gttgaagcat tgctattttg aatttcttgt
    26521 ttacgtgtat tatatgcgtt ttctacttcc gtatcagctg caggtttaac ttttgcttgt
    26581 gtttgaattg cattgatttt atctattact tttgattttg tatcagcaac ttcctgtttt
    26641 gatttaacaa cttggatgtc atgtaaacct tgcttttgag ctgcatctac tgctgcatca
    26701 gcttctgcta cttcttcatt tgttgcattt gaaactgtag cattttgagc ttttctagct
    26761 gttgcagctt gtttaacttc attataggca tccatttttg tttgcgtaga gtcagtaata
    26821 ttttcaattt ttgagatttc ttcgttttta actctttcta aatcttgtgc agtcgttgtc
    26881 gcttcaattg ctttaacacc agaaacttta gctgcattaa tacgttcaat agcttccgct
    26941 acttcttcat ttgttgtatc aggatttaaa ggtgcttgat caatttgtgc ttgaacaact
    27001 tcgtcaaatt cttcaagagc tgctgcttta gctgttgtag caggtgtagt ttgattaata
    27061 tcattgatcg cttttgtttt aatggcttct acttgtgcat ttgttgttgc tgcatcaata
    27121 tctttaattg cctttgtttc aattttacca attttatcat ttgctacatc tttttcatct
    27181 tgtaatgatg catttgactt tttaatttgt tgtttacgag ttgtaactgc ttggataata
    27241 tcttgtttcg cttgaggttt aactgttggt gtaatcacat cttgttctaa cactgcgata
    27301 ccattatctt tttcagttgt gacaccttgt gatgttgttt gtttattaat ttctgcgatt
    27361 gcattatttt tatcattgtt aattttagtc acaagcgctg caacttcatc ttgcgtaact
    27421 tttttacttt gttgtgctgc tgtaaccttt tcttgagctt tagcatctaa ttcagcaatt
    27481 gctgcttgat tatcaattaa tgcagcttgt aatttagtta ctaaaccgtc aatatccgct
    27541 tgagatgcac gatttgcagt ttcaacgtga ttcgcatctt catttaaaat agtgtctgct
    27601 tgttgtttaa gtttattgta ttctgcaatt gatgctgttg tgtaatgact attatcaact
    27661 tgtgagttta cttgttgttg caacgcatct ttgttcattt caaccgctac tgaaaatgga
    27721 tctgcagtta gtgtaacttc tggttgcgca ttattaatta caacatctga agcagtacga
    27781 tatgttaatt tttcattaaa atcaatattt ttaggtgtat cgatattcgc aacattaact
    27841 ttatatgata attttaaaga tttatctggg aataaaactt ctttagtgtg tgtaccacgt
    27901 gccgttgtca caccttggct tgtaaatgta actttatttg cattttgatc ataattaaca
    27961 gtcatatttt tcaatactgt actgtcttca ttaccattag ggaatgttgt agttaatgaa
    28021 tcattaacgt aagttacacc ttgtggtaat gttacttcat atttaaattg atctgctttt
    28081 aatgaagcac caaaattacc attgttaccg atttcagtat taatagtata ttctttattg
    28141 ttagttgctt tcggtgctaa atctctattt ttgacatata aatgtgatcc tgagaataga
    28201 cctacattat caatgaatga atagtattta taaccatctt tatttgttgt aattcttgaa
    28261 gcatcagcaa ctgctgtatt gtcaggaata aattgtaaat ctaaacgatc agcatcagtt
    28321 ggtactttaa ataaatgtgc agtaccagtc ttagcaacat ttacagttgc taattcagta
    28381 tttttcttag catctttaat aactaaattt gttgcacctt gcttactatt cgtttgcata
    28441 gtagtaaagt taataattaa ttctgaatct ggttttacat ttacagtttt tgaaataccg
    28501 ttaaagttac catgatctgt agaatcattt gcattcacac gacctaatgc agccacgttt
    28561 cctttagctt gatagttttg agggttattc ttatcaaaca tatcgcttcg tcttaattct
    28621 gagttaacga aaccaatctt accgttgtta attaatgaat aaccatttac tttatctgta
    28681 acagttacag ttggatcctg tctattctca tctgttgata tggcaggatc atcaaatgtt
    28741 aatgtcgtat taatactgcc ttcaccagta ttgctagcat ttggatcttg agtttgtgcg
    28801 tttgctgcta caggtgctgc tggttgcgct gctgctggag cattcgctgg ctgtgtttga
    28861 tttgccggtg ttgcattatt attaggtgtt gcttggttat ttccttgacc tgcttggttt
    28921 gccggtgttg cttgatttcc aggttgtgct tgtgcagcgt tattcggatc agcttgatta
    28981 ccttgtccag ctggttgtgt atttggttgt gctgctcctc ctgctggatt agcctgtcca
    29041 ccttggtttg ctggttgtac tgctggttgt ccttggttgg caggtgcagc tggctgtgct
    29101 gtaggattag cttgagcacc agcatttgcg ttaggctgtg tattggcatc agctggttgt
    29161 gctggttgat tttgtgcagg ctgattttgc tctgctgcag acgctgttgt cgggttagta
    29221 gatataaaag taacagtggc aattaaagct gaaaaaatac cgacattaaa ttttctgata
    29281 ctaaattttt gttgtctgaa taaattcatt aagtcatcct cctggttgat tattctcgct
    29341 gttaaatgat ttcacttaat caactgttaa gataagtagt agcatctgcg ttaaaaacac
    29401 aaagcaactc tatctaatta aaattaattt tatcatcatt atatattgag taccagtgta
    29461 ttttatatta catattgatt actttgtttt tattttgttt atatcatttt acgtttgtac
    29521 tataaattat ttctagaaac acaaaaaaac cgatgcatac gcatcggctt atttgtaata
    29581 cagtatttat ttatctaatc ccattttatc ttgaaccaca tcagctattt gttgtgcaaa
    29641 tctttcagca tcttcatcag ttgctgcttc aaccatgaca cgaactaatg gttctgttcc
    29701 agaaggtctt actaaaattc gaccttctcc attcatttct acttctactt tagtcataac
    29761 ttctttaacg tcaacatttt cttcaacacg atatttatct gttacgcgta cgttaattaa
    29821 tgattgtgga tattttttca tttgtccagc taattcactt agtgatttac cagtcatttt
    29881 tattacagaa gctaattgaa taccagttaa taaaccatca ccagttgtat tgtaatccat
    29941 cataacgata tgcccagatt gttccccacc taagttataa ttaccgcgac gcatttcttc
    30001 tactacatat ctgtctccaa ctttagtttt attagattta attccttctt gttcaagcgc
    30061 tttgtaaaaa cctaaattac tcataacagt agaaacaatc atgtcattat tcaattcttg
    30121 atttttatgc atttcttgac caataataaa cataatttgg tcaccgtcaa cgatttgacc
    30181 attctcatct actgctatga ttctgtctcc atcgccgtca aatgctaacc caaaatcact
    30241 ttcagtttca actacttttt cagctaattt ttcaggatgt gtagagccac atttctcatt
    30301 gatattatat ccatcaggac tacatccaat tgtttcagta tctgcttcta aatcaccaaa
    30361 taagaatggc gctagtgatg atgttgaacc atttgcacca tctaaaacaa ttttcaaacc
    30421 ttcaaagtta acatctactg ttgattttaa atagctcaaa tatttttgtg ccccttcaaa
    30481 gtaatctgaa taatgtacaa tatcattgcc aactggtctt ggtaattctg ggttttcttg
    30541 atccaacaat gcttcaattt cattttcttg ttcatctgat agtttaaaac catctgatcc
    30601 aaagaattta ataccattat ctgcaactgg attatgagag gctgaaatca ttacacctaa
    30661 ctctgcaccc atatcgcgtg ttaaatatgc aacacctggt gttgaaataa tacctaatcg
    30721 catcacttct gcaccaattg aaatcaaacc agctattaat gctgattcta acatttcacc
    30781 tgaaactcta gtatcgcgac ctacaagtac acgtgggtgt ttttcacctt tgttatgtgc
    30841 tagaacatag ccaccgtatc ttcctaattt aaatgccaat tcaggtgtta gttcttggtt
    30901 tgcgacacct cttactccgt ctgtaccaaa atattttccc attgtttact ctcctttaat
    30961 ttagctattt attttacatt tatataagcc ttcgtttcac ttggttgtgc tttagtgaca
    31021 tgttctggta aattgatttt tacagtcttt tcagttgatt ctgaaatacc atctaaatct
    31081 acttctgcat caacttcgct tatattttgt aagtcatctc gactaccgaa gatttcaatt
    31141 tctttatctt ctaaatcaat cgaacttaac tctttatcat ctgctaaact acctttctgt
    31201 ttaacattta ctttaacctt tttgctaaaa ggctctactt taacttgtaa attcacttca
    31261 ttaggttgaa tcgatacatt taatttattc agttttttat caaaagccgt tacttctgcg
    31321 acatcttttg tgtcaccatt aatcttttta ttagttttaa aagtggcttt taaataagcg
    31381 atatcattca attgttcttc tccacctgtt actttaactg tttgtggtga aacttcttgc
    31441 tttgtaattt tataaagtgg atcaatatca ctctgactta catcaggttg aactgtcatc
    31501 tttttagtta ctttgttttc aagcgtaata tttgctaatt taggttttac agaataatga
    31561 atgtcatcag ctaacccttt aacttgatac ttctcttgat attttccagc tttagcattt
    31621 gataaatcaa tcactactct taaatcttct ggattttcaa tttttattat ctttgattgt
    31681 ggtcctgaaa tagtcacatt aactgtttca ggcgcttttg ttaaatgcaa atctttagtg
    31741 ttataaagaa tttcaacggg tacatcttga atcgttttac tagacttttg accaagatta
    31801 ccagtgttaa agatatttcc aaaaacattg ttaacagata aaaagaaaaa caatgccaaa
    31861 agaaaggcaa taaatctcaa gccccattta ctttctagca tattatttca cacctttctt
    31921 ttgaaagcgt gtgccaaacc aatgttcagc aagcaactct tcaaaaattt cgtttgaaat
    31981 gtctcgtcgt aattttccat caaatgttac cgaaatatca ccagtttctt cagatacaat
    32041 aacggtaaat gcatcagata cttctgaaat accaaccgca gctctatgtc ttgtacccaa
    32101 acttttagat atcttaggac tatcagacaa tggcaaataa cttgctgctg ctgcaatctt
    32161 agtgccttga ataatcattg caccatcatg taaaggtgtg ttaggtataa agacattaat
    32221 taaaagttct tgcgaaatat ttgaatccat tgcaatacct gtttcaatat aatcttgaag
    32281 acctgtttct ttttcaaaga caattaatgc acctatacgt cttttagcca tatattgcac
    32341 agcctttgaa accgattgaa tcaatttctc ttcatcttta ctatacgtat tagaagtata
    32401 gcgttttaaa aagctacctc taccaagttg ttctaacgca cgtctaattt ctggttggaa
    32461 tattactatt aaagctaata ccccccattg aataacgata tcgaataatt tagatgttgc
    32521 agtcaagttc aatatcatac ttatctgctg accaataaca attactaata tccctttaag
    32581 taattgtatc gctttagttc ccttaaagac cgtgatgaga agataaagta cataccaaac
    32641 tatcagtaaa tcaaggatac tcgttacaat ttttaacgta ctgaggtttt gaaaaaagtt
    32701 ggaaaaatcc ataacatctc ctccgggtaa tatttttcca taatacccat tataccaatc
    32761 atttacatca ctgtcatacg aacatatatt taaatagaaa aaatttattt taaagattat
    32821 aactactctt aatcatttta gtgaattaaa aaaagtagtg caaaaagcaa aatatacttt
    32881 atacactaca aatcatttat ttataataaa gtttcaccaa aaaatgttcc aactaatgaa
    32941 accgcttgtt cagcagtatg attattactg tcaatcaatg gatttacttc aactaaatcc
    33001 attgaggaaa ttaaatgtga ttgatgcagt aattccaatg caaaatggct ttctctataa
    33061 ctaagaccac ccaaaactct agtaccagtg cctggcgttt caagcggatc taaagcatca
    33121 acatctaaag ataaatgaac gccatcaaca ttgcgtgact tcaaatattc tattgtattt
    33181 tcaattactt cctttatccc caatttatca atatctgaca tagtaaatgt tttaatatta
    33241 tgatctttga taaattgtct ttcaccttta tctaaatctc tcataccaat tagtacgatg
    33301 ttttctggct tgattacatt actatttaat tctaaaagtt ctttgggacc ttcgcctgtc
    33361 aaaatcctta gaggcatacc atgaatattt ccacttggtg actcttcagg tatatttaaa
    33421 tcaccatgtg catcatacca aataacacct aaattattat aatgtttact tattgctgat
    33481 actgaaccta ccgcaataga atgatctcca ccaagaacta gaggaaatct gttattttca
    33541 attgatgctg aaacctcttt atttaatttt tgattaacat ctataatttc atcataattt
    33601 cttaatcctt tttgttcact atgaaatttt tcaatgttca cagcaggtac cttaatatcc
    33661 cccttgtcat atacatcaag gtctaattgc tttaatcttg aaattaatcc agcatatcta
    33721 attgctgttg gtcctaaatc aacacctaat tttctttgtc caaatgttga tggtgcacct
    33781 ataatatcaa ttgcttttgt ctttgtcata agcgtccccc tttgctctat gtaattaaag
    33841 aataatgtat gcgcttacca ttatcaagca atagctacac atataatctg tttatcttat
    33901 tacttcataa aaaaaagcac ttcccaaaaa tggaaagtgc aagtagtgag ccatagagga
    33961 ttcgaacctc tgaccctctg attaaaagtc agatgctcta ccaactgagc taatggctct
    34021 aatggctggg ctagctggat tcgaaccaac gagtgacgga gtcaaagtcc gttgccttac
    34081 cgcttggcta tagcccaata tatagatggt ggaggggggc agattcgaac tgccgaaccc
    34141 gaaggagcgg atttacagtc cgccgcgttt agccacttcg ctacccctcc agcttattca
    34201 tataattaaa taatcaaaat ggtggagaat gacgggttcg aaccgccgac cctctgcttg
    34261 taaggcagat gctctcccag ctgagctaat tctccaaaat
//
LOCUS       DAHHYJ010000012        33256 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000012 DAHHYJ010000000
VERSION     DAHHYJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33256
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.012"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..650)
                     /locus_tag="NBK22_000434"
     CDS             complement(<1..650)
                     /locus_tag="NBK22_000434"
                     /inference="COORDINATES: protein motif:HMM:NF020442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HCD2413645.1"
                     /translation="MSYDICDFEMNCENSDNDNLNKKSEIICNNLKSELNKCKTHNKE
                     KSWQIFYFIKEFIQSLDLLEEFNFNYFRGQRSNWKVLPGLLRDSTNKEYINHFEQEYK
                     RLAYNYPEELSYLPYDKNNRLERANYLSILQHYGMQTSLLDITKNPFIALLFMVSEEN
                     KNKINKPSFILYEIDENIHHESHLFIRVIKDANNKRIEAQRGAFLCYDYLYSLNIT"
     gene            complement(741..1334)
                     /locus_tag="NBK22_000435"
     CDS             complement(741..1334)
                     /locus_tag="NBK22_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001798987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCD2413646.1"
                     /translation="MTYGDVIDENLLNEFTEDQKEKIKKLVLNNEKGYFIDIEEICSI
                     CNISVEKAKIFGDFDMNDSGVYDEENSTIIYNPLDSITRQRFTIAHELGHAVLGHKGT
                     SFRTKIADKYENILERMKETSANQFAAKLLMPKILVKSLLKEEIEKLSFDSNNLSALD
                     VETLVENIAGKLKVSRESLSYSLKNNNLIRVDDYEHI"
     gene            complement(1512..1676)
                     /locus_tag="NBK22_000436"
                     /pseudo
     CDS             complement(1512..1676)
                     /locus_tag="NBK22_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
     gene            complement(1664..2155)
                     /locus_tag="NBK22_000437"
     CDS             complement(1664..2155)
                     /locus_tag="NBK22_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD2413647.1"
                     /translation="MVSDFESNDKITEVELLMHYNPKVINSKIKAMRSQIESLYHLNM
                     NHVITNDNDMLVSVSYPLDKLVLYIIEEKDKLEYYMKTAQDRLNLFKDIIKNYSENEQ
                     QDVMRYMLSSGKVKNEGVIERLKVDIYKVESRKRQERQNQREELHRIEFNKHLEQVKK
                     ELS"
     gene            complement(2333..3391)
                     /locus_tag="NBK22_000438"
     CDS             complement(2333..3391)
                     /locus_tag="NBK22_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HCD2413648.1"
                     /translation="MKEFKEQFGYQLSNFDDMDIKGYANLYQKDIGKDVSMIEQGLKQ
                     LSITETEVLLPEQINKKLLGVLNMNEVNQSSNTWVGTLTKQLVSSENNFNIQELPTAR
                     KEVFKELLVNCELPQSLREVITITDDEHVESIPSLTYIKDKLATNGIELSLNGSSKYF
                     DRREGHIYTEVSDSVVHGSDRTLDDLLKEIFINECVAYETTLLLDKNNSSGLIDKGNQ
                     DLSLYNQGIKEVSNTSIYDGIKQAMKDIPQTFRRNVSIVMNTEHHDKLIKELAQMGLG
                     GLAGDLSNLFNVTNVVVTDDAHDVFVGDFGHAIYAKYEPIMYNKKKQALKGIYQFALN
                     YVFDIKIIPELLRIVNIK"
     gene            complement(3378..3584)
                     /locus_tag="NBK22_000439"
     CDS             complement(3378..3584)
                     /locus_tag="NBK22_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413649.1"
                     /translation="MLDHKNESVKRVFKYRDKQELLNKYHELLDKYFLHNISFYEEDK
                     NNERIYGLVVHTKNKLGDEDYERV"
     gene            complement(3809..4153)
                     /locus_tag="NBK22_000440"
     CDS             complement(3809..4153)
                     /locus_tag="NBK22_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD2413650.1"
                     /translation="MNRSRMKQIILEYIKSNGSTSFVEIENVFEEQGFKYKGNGAYTS
                     GNHKNIIFWVGWNEEAFNIVADLKRDGLIEMQICPPMYYLIDGKGLKLPIVKNKNIKI
                     DHWLPVIFSLVN"
     gene            complement(4824..7196)
                     /locus_tag="NBK22_000441"
     CDS             complement(4824..7196)
                     /locus_tag="NBK22_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD2413651.1"
                     /translation="MAIKKKDRIIRVKELEIPQELKLVPNWVLWRAEWNEKQQNFGKV
                     PYSINGYRASTTNKKTWCDFESVSIEYEVDEQYSGIGFVLSDGNNFVCLDIDNAIDKK
                     GQINSELALKMMQLTYCEKSPSGTGLHCFFKGKLPDNRKKKRTDLDIELYDSARFMTV
                     TGCTIGQNDICDNQEVLNTLIDEYFKENLPANEVVREESNTNIQLSDEDIINIMMKSK
                     QKDKISNLLQGNYESYFESSSEAVQSLLHYLAFYTGKNKQQMERIFLNYNNLTDKWES
                     KRGNTTWGQLELDKAIKNQKTIYTKSIDEFNVIPQGSKDVKQLLNQLGHEERTKMEEN
                     WIEEGKRGRKPTTISPIKCAYILNEHLTFILFDDEENTKLAMYQFDEGIYTQNTTIIK
                     RVISYLEPKHNSNKADEVIYHLTNMVDIKEKTNSPYLIPVKNGVFNRKTKQLESFTPD
                     YIFTSKIDTSYVRQDIVPEINGWNIDRWIEEIACNDNQVVKLLWQVINDSMNGNYTRK
                     KALFLVGNGNNGKGTFQELLSNVIGYSNIASLKVNEFDERFKLSVLEGKTAVIGDDVP
                     VGVYVDDSSNFKSVVTGDPVLVEFKNKPLYRATFKCTVIQSTNGMPKFKDKTGGTLRR
                     LLIVPFNANFNGIKENFKIKEDYIKNQQVLEYVLYKAINLDFETFDIPDASKKMLEVF
                     KEDNDPVYGFKVNMFDQWTIRKVPKYIVYAFYKEYCDENGYNALSSNKFYKQFEHYLE
                     NYWKTDAQRRYDNEELAKRIYNFNDNRNYIEPIESGKNYKSYEKVKLKAI"
     gene            complement(7288..7587)
                     /locus_tag="NBK22_000442"
     CDS             complement(7288..7587)
                     /locus_tag="NBK22_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCD2413652.1"
                     /translation="MNWEIKNLMCDLKILKEKFEDLKDNHGSHFEDLYLHEPNHTLNK
                     DDVIKEGFSYHERRIHNDQMFDLLLLYTEKFDELITRFDALEKASSFADQSQDNA"
     gene            complement(7588..7812)
                     /locus_tag="NBK22_000443"
     CDS             complement(7588..7812)
                     /locus_tag="NBK22_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413653.1"
                     /translation="MKNEQKEVIQDIYNTLEAVAYNTSMEYIHNCVDGKKEWMENVNR
                     EEHLQAIIEWALQQIENNFDFENDTEMEEL"
     gene            complement(7802..7951)
                     /locus_tag="NBK22_000444"
     CDS             complement(7802..7951)
                     /locus_tag="NBK22_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413654.1"
                     /translation="MKLYLVYVTLTSFLTILLLAISNMYVAFSVYGMMVTYGFNLTGG
                     LENEK"
     gene            complement(7998..8258)
                     /locus_tag="NBK22_000445"
     CDS             complement(7998..8258)
                     /locus_tag="NBK22_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD2413655.1"
                     /translation="MSQLLSEQASQQLVNSIVDIAEKIALEKVKQSRKRYLIQKEVME
                     EYNVTHKVITEWEIMGLRKVKIGKTIRYDRQDIENVIEQMKN"
     gene            complement(8259..8495)
                     /locus_tag="NBK22_000446"
     CDS             complement(8259..8495)
                     /locus_tag="NBK22_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2413656.1"
                     /translation="MNENVKLLLRAARINNGMSQKKAASLLGVHYQTLASWEKDSSDL
                     SRSKMLLMEKIYSIPLEYIFFGNENEFIRTKRRG"
     gene            8628..9014
                     /locus_tag="NBK22_000447"
     CDS             8628..9014
                     /locus_tag="NBK22_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2413657.1"
                     /translation="MPRPSLSYHEKILRKTISKNLKRLSEGKTQAEISQHTNIPTSTL
                     SGYFAERSTINPENTEKLATFFNVSLEKIDPRFSRNMLFLEDGDDEEYSLTVEILDMI
                     EQLNKENKYKAFINTQKLLINQEKTN"
     gene            9098..10234
                     /locus_tag="NBK22_000448"
     CDS             9098..10234
                     /locus_tag="NBK22_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2413658.1"
                     /translation="MIKKYKKKDGSTAYMFVAYLGTDPITGKQKRTTRRGFKTEREAK
                     IAEAKLQTEVSQNGFLNNDITTFKEVYELWLEQYQNTVRESTYQRVLTLFDTAILEHF
                     QDVPIKKITVPYCQKVINKWNKKYSDIKAIRIYTSNVFKYAVSLKIIVDNPFAHTKAP
                     RKKEAQQDASTKYYSSDELKQFLTFVEDDPLYYAIFRTLAFTGFRRGELMALTWNDID
                     FTKQTISINKTCARGANYKLVIQEPKTKSSHRTISIDDKTASVLKSWRTHQRVESLKY
                     GHNTSDKHQHVFTTVRDNKQLYPEHCNKALDLICEKNSFKRIKVHGFRHTHCSLLFEA
                     GLSIQEVQDRLGHGDIKTTMDIYAHVTEKQRDQVADKFAKYINF"
     gene            complement(10328..11869)
                     /gene="guaA"
                     /locus_tag="NBK22_000449"
     CDS             complement(10328..11869)
                     /gene="guaA"
                     /locus_tag="NBK22_000449"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCD2413659.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINTKSDELFAGLPAEQTVWMSHSDKVIEIPEGFEVIADSPSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCRGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(11894..13360)
                     /gene="guaB"
                     /locus_tag="NBK22_000450"
     CDS             complement(11894..13360)
                     /gene="guaB"
                     /locus_tag="NBK22_000450"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCD2413660.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(13398..14666)
                     /locus_tag="NBK22_000451"
     CDS             complement(13398..14666)
                     /locus_tag="NBK22_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCD2413661.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILLLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(14666..15244)
                     /gene="xpt"
                     /locus_tag="NBK22_000452"
     CDS             complement(14666..15244)
                     /gene="xpt"
                     /locus_tag="NBK22_000452"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCD2413662.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHKRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(15475..15577)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15757..16164
                     /locus_tag="NBK22_000453"
     CDS             15757..16164
                     /locus_tag="NBK22_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCD2413663.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLHLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            16307..16969
                     /locus_tag="NBK22_000454"
     CDS             16307..16969
                     /locus_tag="NBK22_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413664.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            17087..18043
                     /locus_tag="NBK22_000455"
     CDS             17087..18043
                     /locus_tag="NBK22_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413665.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESTNKVEKNAVTEDKADSKDSKVAGVNTSTDAKTDTKADNAGDGTTT
                     KVDKEDQISATEAIEKASVEQSKNGNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSSEAKVENNSTASQSEMAEPKPQEEKKGFFARLFNL"
     gene            complement(19035..19133)
                     /locus_tag="NBK22_000456"
     CDS             complement(19035..19133)
                     /locus_tag="NBK22_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413666.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNLVEKGEKG"
     gene            19218..20606
                     /locus_tag="NBK22_000457"
     CDS             19218..20606
                     /locus_tag="NBK22_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCD2413667.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKSA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITTYI
                     IHLVILSLLGISPIRYVKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FDSNDNAALTSH"
     gene            complement(20686..21441)
                     /locus_tag="NBK22_000458"
     CDS             complement(20686..21441)
                     /locus_tag="NBK22_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HCD2413668.1"
                     /translation="MSEHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPVFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            21933..22502
                     /gene="ahpC"
                     /locus_tag="NBK22_000459"
     CDS             21933..22502
                     /gene="ahpC"
                     /locus_tag="NBK22_000459"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCD2413669.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            22518..24041
                     /gene="ahpF"
                     /locus_tag="NBK22_000460"
     CDS             22518..24041
                     /gene="ahpF"
                     /locus_tag="NBK22_000460"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCD2413670.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDEKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTGIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            24156..25775
                     /locus_tag="NBK22_000461"
     CDS             24156..25775
                     /locus_tag="NBK22_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413671.1"
                     /translation="MLDVHFIYPRKLQNACEYFNLPTQKEGFEVTMHALRPENIHKIY
                     HDMDKTEQKFIETLISNGNPVTIINKRDVNILKLTNHLGFVYQLNEDEFFVNNFFLRY
                     INSEVLDNAYGEFESLDTINHMNLNEEVLRVRQHTRLNDVTLEHALLLKTNDELKTIC
                     RQYGIKGFSNKNKQNLVELIMTAFFNNNHIIEQVFSNTTLFEFDILSSILESDANYRL
                     NNELIEFEKLQLINFNNFLTSHFYYYYDFEYDCLILPNDVRKHIENFVNQVGNGDFEK
                     AVNQFVDEQTIEKSLAEFENALELSFMDDEVDLDMLLKDDDFEDFGTTDSLRSDAITQ
                     LISEIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLTQRFYDATFSKEELQYSLD
                     TLAGTELYYIEDDFVFHPVLFDVVESEASGIDISDEGYYVPASVEELIYYDRHHYLKS
                     DSEIKAFTSYLRKQINMKNDIEKEKYVNEIIMLLRTIPTSKLIQPIMQLFIDNAILNN
                     VQNDALLIEKVTHARNHLRLWSLQGHRDIPN"
     gene            25848..26474
                     /locus_tag="NBK22_000462"
     CDS             25848..26474
                     /locus_tag="NBK22_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCD2413672.1"
                     /translation="MKKRLLLSTFLASTLILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            26730..27113
                     /locus_tag="NBK22_000463"
                     /pseudo
     CDS             26730..27113
                     /locus_tag="NBK22_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868247.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27179..27760)
                     /locus_tag="NBK22_000464"
     CDS             complement(27179..27760)
                     /locus_tag="NBK22_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD2413673.1"
                     /translation="MTIYLVRHGESKSNYDNKHFRSYFCGQLDVPLTDTGTKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDSNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNNVSREEAVDLKVENCKPYIIE"
     gene            27938..28147
                     /locus_tag="NBK22_000465"
     CDS             27938..28147
                     /locus_tag="NBK22_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413674.1"
                     /translation="MTTQSFIFMNIILLDDATQRHPFFIYQILAFLFPTSPHVNLLLF
                     NPILPTFSNIIIHIDHFHANKKDDN"
     gene            complement(28190..28441)
                     /locus_tag="NBK22_000466"
     CDS             complement(28190..28441)
                     /locus_tag="NBK22_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD2413675.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWLG
                     GTLLGQWGPEIGRIYILPALIGSIILIAIVTLILRAMRK"
     gene            28741..29004
                     /locus_tag="NBK22_000467"
     CDS             28741..29004
                     /locus_tag="NBK22_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2413676.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDAPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(29142..29714)
                     /locus_tag="NBK22_000468"
     CDS             complement(29142..29714)
                     /locus_tag="NBK22_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD2413677.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(29895..30263)
                     /locus_tag="NBK22_000469"
     CDS             complement(29895..30263)
                     /locus_tag="NBK22_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD2413678.1"
                     /translation="MKTILKTITYLALTIIGAYAALFILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYTQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(30632..31243)
                     /locus_tag="NBK22_000470"
     CDS             complement(30632..31243)
                     /locus_tag="NBK22_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD2413679.1"
                     /translation="MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQ
                     DKQLQKVEEVPNNSEKVLVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVAGRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKNRENVEINTDDIKNVTFELVKSVNDIEQV"
     gene            31450..32151
                     /locus_tag="NBK22_000471"
                     /pseudo
     CDS             31450..32151
                     /locus_tag="NBK22_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270135.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            32190..33128
                     /locus_tag="NBK22_000472"
     CDS             32190..33128
                     /locus_tag="NBK22_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCD2413680.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKCLVLKLITENNQEDGY
                     KIIDEFLCIDKSYSNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPEKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRSPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
BASE COUNT        10948 a         5940 c         4812 g        11556 t
ORIGIN      
        1 tctgttatat tcaaactata tagataatca taacatagaa atgctcctct ttgcgcttca
       61 attcgtttgt tatttgcatc ttttataact ctaataaata gatgagactc atgatgaata
      121 ttttcgtcta tttcatacaa aataaaacta ggtttattaa ttttattttt attttcttcg
      181 ctaaccataa ataataatgc gataaatgga ttttttgtaa tatcgagcaa acttgtttgc
      241 attccataat gctgcaatat agataaataa ttagcccttt ctaatctgtt atttttatca
      301 taaggtaaat aagagagctc ttcagggtaa ttataagcaa gtcttttata ttcttgttca
      361 aaatgattta tatactcttt attagtgcta tctcttagta aacctggcaa tactttccaa
      421 ttagaacgct gccctctaaa ataattaaaa ttaaattctt ccaatagatc taaagactgt
      481 ataaattctt taataaaata aaatatttgc catgattttt ccttattatg agttttacat
      541 ttatttaatt ctgattttaa attattacat ataatttcac tttttttatt tagattatca
      601 ttatcactgt tttcgcagtt catctcaaaa tcacaaatat cgtatgacat tttctctttt
      661 tttcttcatt aggtataata ataagtttaa attttatgtc attgtacaat ttaactttat
      721 tatttttttc ttttatttct ttatatatgt tcataatcat ctactctaat taaattatta
      781 tttttcaaac tatagcttaa agattctcga gaaactttta attttccagc tatgttttca
      841 actaatgttt ctacatccaa agcactcaaa ttatttgaat caaaacttag tttttctatt
      901 tcttctttta gaaggctttt cactaaaatt ttaggcatta gtaatttagc tgcaaattga
      961 ttggcgcttg tttctttcat tctctctaaa atattttcat acttatcagc tattttagtt
     1021 ctaaaagatg tccctttatg acctagtacc gcatgtccca actcatgggc aatagtaaat
     1081 ctttgacgcg taatcgaatc tagtgggtta tagattatag tgctattttc ttcatcataa
     1141 actcctgaat cattcatatc aaaatcacca aatattttag ctttctcaac agaaatatta
     1201 caaatagagc atatttcttc aatatcaata aagtaacctt tttcattatt taatacaagt
     1261 tttttaattt tttctttttg atcttcggta aactcattca ataaattctc atcaataacg
     1321 tctccataag tcatatcaat cactttgaca cacctccatc tattcatatt aaaaattctt
     1381 aaacttcaaa ttatcactga atagcaaaat attgcattat aattattaaa ttaagtctcc
     1441 tttatctagt agtttatata taataataaa ttatttctgc aatttttact atatatcgac
     1501 atacaaaaat gtcaatcctc taacggaata tcatccacaa tcacaggatg actaggatta
     1561 gcattagata cctcttttac agttttgtct aactcctcat catctccgtc ccattcacca
     1621 atattaatga atataggaat attcccgtta atatcatgct aatctatgaa agttcttttt
     1681 taacttgttc taaatgctta ttaaactcta ttcgatgtaa ttcttctcgc tggttttgtc
     1741 gttcttgacg ctttctactt tcaactttat aaatatctac ttttaaacgt tcaatgactc
     1801 cttcattttt aactttccca ctacttaaca tgtacctcat aacatcctgt tgttcatttt
     1861 cagaatagtt tttgatgata tctttaaaca aatttagcct atcctgagct gttttcatgt
     1921 aatattcaag tttatctttc tcttctataa tataaagtac taatttatct aatggatatg
     1981 atacagatac cagcatatcg ttatcattcg tgatcacatg gttcatgttc aagtgatata
     2041 agctttctat ttgtgatctc atcgctttaa ttttgctatt aatgactttc ggattgtaat
     2101 gcattaaaag ttctacttca gtgattttat cattgctttc aaagtcagat accaaataac
     2161 cacaccttta catatcgaat ttaatattta taagtatctg aaaaagggat aaatcaagtt
     2221 atcccccttt cataaaagtt tatccgcgtt gcaagcgaag ggcccccgcc ggtacccgtc
     2281 gaaaaaacat tttaagccga tgggtagggg gctataaaaa taaattattt ttttatttaa
     2341 tattaactat gcgcaataac tctggaatta tttttatatc aaacacatag ttcaatgcaa
     2401 attgataaat accttttaat gcttgtttct ttttgttata cataataggt tcgtatttag
     2461 catatatggc atgtccaaag tctccaacaa agacatcatg cgcatcatca gtaaccacta
     2521 cattagtcac attaaatagg ttacttaaat cacctgcaag tccacctaat cccatctgtg
     2581 ccaactcttt gatgagctta tcatgatgtt ctgtgttcat cacaatagat acattacgcc
     2641 taaacgtttg tggtatatct ttcatcgctt gcttgatacc atcgtatatt gatgtgttac
     2701 ttacttcttt gataccttga ttataaagtg ataagtcttg gttaccttta tcaattaaac
     2761 cacttgaatt attcttatct aataacaatg ttgtttcata tgcgacacac tcattaataa
     2821 atatctcttt aagtagatca tctaatgttc tatcagagcc atgaactaca ctgtctgata
     2881 cttctgtgta gatatgtccc tcacgtctat caaagtattt actactgcca ttaagtgata
     2941 gctcaatacc gttagtagct aacttatctt taatgtatgt tagtgatggt atagattcaa
     3001 catgttcatc atcagtaatg gtaatcacct cacgtagtga ttgaggtagc tcacaattca
     3061 ccaataactc tttaaatact tctttacgtg cagttggtaa ctcttgaata ttaaagttat
     3121 tctcactact aactaattgt tttgttaagg tacctaccca tgtattactt gattgattta
     3181 cttcattcat atttaacact cctaataatt ttttgttaat ttgctctggt aataaaactt
     3241 ctgtttcagt aatacttaat tgctttaacc cctgttcaat catacttaca tctttaccta
     3301 tatccttttg atatagattg gcgtatcctt taatatccat atcatcaaag ttagatagtt
     3361 gatacccaaa ttgttcttta aactctttca taatcttcat ctcctaattt gtttttagta
     3421 tgaacaacta aaccatatat acgttcgttg tttttatctt cttcatagaa cgaaatgtta
     3481 tgaagaaagt atttgtctaa taattcatga tacttattta atagttcttg cttatctcta
     3541 tacttaaaca cacgttttac agattcattt ttatgatcta gcacgtcttg cctctctcct
     3601 tttaatctga tagatataca ttttaataag ttcaaaatca gcacgctttt ttgtatagcg
     3661 ccttacaacg cgatttatat atttcgtatt cattaattga acgttcacag gtatatgttt
     3721 gattatttga tatgcaattc ttttatgatc aataaatata cacactccca tatctatact
     3781 tgatatttta aatgcttatc tgcatgtttt aatttactaa actaaatatt actggtagcc
     3841 aatgatctat ttttatattt ttattcttta cgattggtag ctttagacct ttaccatcaa
     3901 taagatagta cattggcgga caaatttgca tttcaattag cccgtcgcgt tttaaatctg
     3961 ccacaatatt aaacgcttct tcattccaac ccacccaaaa tataatattc ttatggttgc
     4021 cactggtata tgcgccgtta cctttgtatt taaaaccttg ttcttcaaat acattctcaa
     4081 tctctacaaa cgaagtgctg ccattgctct ttatatattc taaaatgatt tgtttcattc
     4141 tacttctatt catgttactc tagtcctcca atctatccag ttactaaagc aaccacgtta
     4201 gatactaaaa accactgaat ttatgttttt caataaccac tgtaaacaca gtgatgtcaa
     4261 tacatacagg ctgttggtta ctgagttact gaaaattcac ggtattttat ataatctttt
     4321 attaccttaa ttctctatta tacatttcta ctaaaaagaa ataatttata gtaactcagt
     4381 aactttttgt caaaaagact aatatgaaag taaaaactgt ggttactgaa ttctagaatt
     4441 ttagtatctt ttagtgactt aaacgttttt tagtaacttt agtatcttag gcaattttgt
     4501 ataactgcaa gtctcttccg attatgtgca actgaaattt acagctatgc aactcgtttt
     4561 aaattttcca gttgcaattt ttttcttact gtcacaaggg gttaatccac ttttgcaact
     4621 atgcaaccga aatcacacaa attagtttat ataatttttg atactatttc ttatttatat
     4681 tttggttatt tacttttcta ctaataagtt atagattttg agttgctcag ttgcataagc
     4741 gccctaaacc cttgatataa ctgtgattat aggtgcaacc cgttttaaaa ttttcggttg
     4801 cattcggttg cattcggttg caactatatt gcttttagct tcaccttttc atacgattta
     4861 tagttttttc cactttcaat aggttcaatg taatttctat tgtcattaaa gttgtatatc
     4921 ctcttagcaa gttcttcatt gtcatatctt cgctgtgcat cagttttcca ataattctct
     4981 aaataatgtt caaattgttt ataaaacttg tttgaactca atgcattata gccattttca
     5041 tcacaatatt ctttataaaa tgcgtataca atatatttcg gcacttttct aatagtccat
     5101 tgatcaaaca tatttacttt aaacccataa actggatcgt tatcttcttt aaatacttca
     5161 agcatttttt tagatgcatc aggaatgtca aaggtttcaa aatctaaatt aattgcttta
     5221 taaagcacat actctagcac ttgctgattt tttatatagt cttctttgat tttaaaattc
     5281 tctttaatgc cattaaaatt ggcattaaac ggtactatca ataaccttct taaagtcccg
     5341 cctgttttgt ctttaaattt aggcattcca ttcgttgatt gaataactgt acacttaaaa
     5401 gtcgctctat ataagggttt atttttaaac tcaaccaaca ctggatcgcc agtaactaca
     5461 cttttaaagt ttgaagaatc atcgacatac acaccaactg gtacatcgtc tccaattact
     5521 gctgtcttgc cctctaacac actcaattta aaacgttcat caaactcatt cacttttaag
     5581 ctagcaatat tgctataacc tattacatta gacaataatt cttgaaatgt acctttacca
     5641 ttgttaccat taccaaccaa aaataatgct tttttacgtg tgtagtttcc attcattgaa
     5701 tcattaatta cttgccacaa taatttaaca acctgattat cattacaagc aatctcttct
     5761 atccaccgat ctatattcca gccatttatt tcaggtacta tatcttgcct tacatacgat
     5821 gtatctattt tactggtaaa tatataatca ggtgtaaatg attctagttg cttcgtttta
     5881 cggttaaata caccattttt aactggtatt aagtatggtg agttagtttt ctcttttata
     5941 tctaccatat tggttaaatg ataaataact tcatcagctt tgttgctatt atgtttgggc
     6001 tctaaatagg aaatcactcg ttttataatt gtagtgttct gtgtatatat cccttcatca
     6061 aattgataca tagctaactt agtattttct tcatcatcaa aaagtataaa tgttaaatgc
     6121 tcattcaaaa tatatgcaca ttttataggg ctaattgttg taggctttcg ccctcgtttt
     6181 ccttcttcaa tccaattttc ttccattttt gttctttctt catgccccaa ttgattcaac
     6241 aactgtttaa catctttact cccctgtggt ataacattaa attcatctat cgatttagta
     6301 taaattgtct tttgattctt tatagcttta tctaactcaa gctgtcccca agtcgtatta
     6361 cctcgtttac tttcccattt atccgtaaga ttattgtagt ttaaaaatat acgctccatt
     6421 tgctgtttgt ttttacctgt gtaaaacgct aagtagtgta ataagctttg tactgcttcg
     6481 cttgaactct caaaatatga ttcataattg ccttgtagta agttgctaat tttatctttt
     6541 tgtttagatt tcatcataat gtttataata tcttcatcag ataattgtat attggtatta
     6601 gattcctctc tcacaacctc atttgctggc aaattctctt taaagtattc atcaatgaga
     6661 gtatttaata cttcctgatt atcacaaata tcattttgac caattgtgca tcctgtaaca
     6721 gtcataaacc ttgctgaatc atataactct atgtctaaat ctgttctttt ctttttacgg
     6781 ttatctggta gtttaccttt aaagaaacaa tgtaatcctg taccacttgg agacttttca
     6841 caatatgtga gttgcatcat ttttaatgct aattcagaat tgatttgtcc ttttttatca
     6901 attgcattat caatatctag gcaaacaaaa ttattaccat cacttaatac aaaacctata
     6961 ccgctatatt gctcatcaac ttcatattca atacttacac tttcaaagtc acaccatgtt
     7021 tttttattgg ttgtactagc tctgtaacca ttaatactat atggtacttt tccaaaattt
     7081 tgttgcttct cgttccattc agctcgccat aatacccaat tgggcactaa ttttaattct
     7141 tgcggtattt ctaattcttt aactctaata attctatctt ttttcttaat tgccacagaa
     7201 tgcctccttt ctaatctgat ttctctgtat tttcaatgca actaatgtta aaatattaat
     7261 ggtatgattt tataaatttt tattttttta tgcgttatct tggctttggt cggcgaaaga
     7321 tgacgctttt tctaatgcat caaacctcgt tattaattca tcgaattttt cagtgtatag
     7381 aagtagcaaa tcaaacattt gatcattgtg aatacgtctc tcatgatatg aaaatccttc
     7441 tttaataaca tcgtctttat ttaaggtatg atttggctca tgtagatata aatcttcaaa
     7501 atgcgagcca tgattatcct ttaaatcctc aaacttttct ttcaatatct ttaaatcaca
     7561 cattaaattt ttaatttccc aattcattta taattcctcc atttcagtat cattctcaaa
     7621 atcaaagtta ttttctattt gttgtaatgc ccactcgatt atggcttgta agtgttcctc
     7681 acgatttacg ttttccatcc attctttctt accatctacg caattatgga tgtattccat
     7741 tgatgtgtta taagctactg cttctaaagt attatagatg tcttgaataa cttctttttg
     7801 ttcatttttc attttctaat cctcctgtta aattaaatcc ataagttacc atcatgccgt
     7861 acacactaaa agcgacatac atgttagata ttgctagtaa taaaattgtt aaaaacgatg
     7921 ttaaggttac ataaactaag tacaatttca ttgttttacc tcctgatagg ttagagttct
     7981 cacgcgttgc aggcgtttca atttttcatt tgttcaatta cattttcgat atcttgtcga
     8041 tcatagcgaa ttgttttacc aatcttaact ttacgaagtc ccattatttc ccactcggtt
     8101 ataaccttgt gagtcacgtt gtattcttcc atgacttctt tttgaatgag atatctctta
     8161 cgcgattgtt taactttttc taaagctatt ttttcggcaa tatcaactat gctattaact
     8221 aactgttgac tagcttgttc actaagtaat tggctcattt aacccctcct ctttgtacga
     8281 ataaactcgt tttcattacc aaaaaaaata tattccagcg gtatgctgta tattttttcc
     8341 atcaatagca ttttagaccg tgataaatca gaactatctt tttcccaaga tgctaaagtt
     8401 tgataatgta cacctaataa gctagccgcc tttttttgac tcataccatt atttatcctt
     8461 gctgctctga gtaataattt tacgttttca ttcattaatc taacctcctc acaagtcact
     8521 ataaacgcgt ttattcgttt tgtcaacgtt taaattcgtt attttacata ttttctattg
     8581 ttttaatcgt atttattcgt taatataacg taaaggagtg ataaaaaatg ccaagaccct
     8641 cattaagtta ccacgaaaaa atcttaagaa aaactatttc taaaaaccta aaacgattgt
     8701 ctgaaggaaa gacgcaagca gaaatatccc agcatactaa tataccaact tcaactttat
     8761 caggctattt tgctgaacga tcaacaatca atcctgaaaa tacagaaaaa ttagcaacct
     8821 ttttcaatgt aagccttgag aaaattgatc ctcgtttttc tcgaaatatg ttatttttag
     8881 aagatggtga tgatgaagag tactctttaa cagttgaaat tttagacatg attgaacagc
     8941 ttaataaaga aaacaaatat aaagccttta ttaacacaca aaaattattg ataaatcaag
     9001 aaaaaactaa ttaatcatca cgtcacaagg tagccattcc ttaacggcgt gtcaatccaa
     9061 tctcacgcgt tgcaggcaat agaaaggatt gatttatatg attaaaaaat ataagaagaa
     9121 agacggttcg actgcctata tgtttgtcgc atatttaggt actgatccaa ttactggtaa
     9181 gcaaaagaga accactagac gaggctttaa gactgaaaga gaagctaaaa ttgctgaggc
     9241 aaaacttcaa acagaagtaa gtcaaaatgg ctttctaaac aacgatataa cgacgtttaa
     9301 agaagtatat gagttgtggc ttgaacagta tcaaaatact gtaagagaaa gcacatatca
     9361 acgtgtttta actttatttg atacagctat attagaacat ttccaagatg tacctattaa
     9421 gaaaataacc gttccatatt gccaaaaagt tattaataaa tggaacaaaa agtattctga
     9481 tattaaagct atacgaatat acacatctaa tgtgtttaaa tatgccgtaa gccttaagat
     9541 aatcgttgat aatccgtttg cacatacaaa agcacccaga aaaaaagaag cgcaacaaga
     9601 cgcatctaca aagtattatt caagtgatga attgaaacag tttttaactt ttgttgaaga
     9661 tgacccatta tattatgcta tttttcgaac attagctttt actggcttta gacgtggaga
     9721 attaatggct ttaacatgga acgatattga ttttactaaa caaaccatat ctattaataa
     9781 gacttgcgca agaggtgcaa attataaact ggtaatacaa gagcctaaaa caaaatcatc
     9841 tcacagaaca ataagtattg atgataaaac tgcaagtgta ttgaaatcat ggcgcactca
     9901 tcaaagagtg gagtccctta aatatggtca caatacctct gataaacatc aacatgtttt
     9961 cactactgta cgtgataata aacaactata tcccgagcat tgcaataaag cattggactt
    10021 aatttgtgag aagaatagtt ttaagagaat taaagttcat ggttttagac acactcattg
    10081 ttctcttttg ttcgaggctg gcctttctat tcaagaagta caagacagat taggtcacgg
    10141 ggacatcaag acaactatgg atatttacgc tcacgtaact gaaaaacaac gtgatcaggt
    10201 ggctgataaa ttcgcaaaat acatcaattt ttaagcatac cgtattcaaa acgtattcaa
    10261 cgcaaaataa aaaaggctgg aaaccgcata atcacggtaa ctccagccta tcatttgcta
    10321 tatataatta ttcccactca attgtgcttg gtggttttga tgtaatgtca tagactacgc
    10381 ggttgacgtg atctacttcg tttacgatac gactagaaat cttttgtaag acttcccaat
    10441 cgatgcgtgc gaagtcactt gtcataccgt cgatagatgt tactgcacga atacctactg
    10501 tgtgatcata cgtacggtag tctcccataa cacctactga ttgaatgttt ggtaacactg
    10561 tgaagtattg ccaaatttct ctttcaagac cttcttctct aatcacttgg cgtaaaatcg
    10621 cgtctgattc tctaacgatt tctagtttat cttcagtaat ttctccaagt acacgaatac
    10681 caagaccagg tcctgggaat ggttgtctcc atactaaatg ttctggaata cctaactcaa
    10741 tacctaattt acgtacttca tctttaaaca atgtattgat tggttcgatt aattcgaatt
    10801 ccatgtcttc tggtaatcca ccaacattgt ggtgtgattt gattgtttgt gctgttttag
    10861 tacctgattc gatgacgtct gtatatagtg ttccttgcgc aaggaagtct acacctttca
    10921 gttttgatgc ttcatcatca aatacgtata caaattcatt accaatgatt ttacgttttt
    10981 gttcaggatc tgaaacacct tttaatttat tcataaagcg atctttcgca ttaacacgaa
    11041 taatattcat gttgaaacct tcaccgaatt gctccataac catgtcgcct tcacctttac
    11101 gaagtaagcc atggtctaca aagatacatg ttagttgatc acctattgct ttatgcaata
    11161 gtacagctac aacagatgaa tctacgccgc cactcatcgc acataataca cgacggtctc
    11221 ctacgcgttg acgaatcttt tcaatttcga tttcgataaa gttttccatt gtccattgac
    11281 ctctacaatc acaaacacga cggacaaaat tatttaataa atcattacca tattctgtat
    11341 gacgtacttc tggatggaat tgaacaccat aaatgcgacg tttcttatct tcgattgctg
    11401 catagtctgt gcttgggcta tcagcgataa cttcaaagcc ttctggaatt tcaataactt
    11461 tatcagaatg actcatccaa acagtttgtt ctgctggtaa gccagcgaat aactcatctg
    11521 acttcgtatt aatgattgct ttaccgtatt cacgttcatt ggcacgttca actttaccac
    11581 ctaataattt agtagttaat tgcatgccgt aacaaatacc aagtactgga attcctaaat
    11641 tatatatttc cggatcaatt gtaaatgaac cttcttcata aactgaattt ggaccacctg
    11701 ataagataat accttttgga ttcattttct taatttcttc aattgaaatt tcatgatcgt
    11761 gtaattcact ataaacgccc atttcacgaa ttcggcgtgt aattaattgg ttgtattggc
    11821 taccaaagtc taagacaagg attaactctt gttcttttgc catttccata tttgtcgttc
    11881 tcctttatct taattagaat gagtagttcg gtgattcttt cgtaatttga atattatgtg
    11941 ggtgactttc tgctaaacca gcagggccca tacgtgtgaa ttgtgcttct tcgcgtaatt
    12001 ctcttaaatc gtgtgaacca gtataaccca taccagcacg cacaccgccc attaattggt
    12061 aaattgtatc ttgtaacgca cctttatatg ccgtacgtcc ttcgatacct tcaggaacaa
    12121 atttcttagg cgctttgtcc tcttggaagt aacggtcgtt tgaacctttt tccatcgcac
    12181 ctaaagagcc cataccacgg tatactttat attgtctacc ttggaaaatt tctgttgcgc
    12241 ctgggctttc ttcagtacct gctaataagc tacctaacat aaccgcatgt ccaccagcag
    12301 ctaatgcttt aatgatatct cctgagaatt taataccacc atcagcaatg atagctttac
    12361 catgtttgcg tgcttcagtt gcacaatcat aaattgctgt aatttgtggt acaccaacac
    12421 ctgctacaac acgtgtcgta caaattgaac ctgggccaat accaacttta acaatatctg
    12481 cacccgcttc aaataaatcc tttgttgctt ctgcagttgc tacgttacct gctactaatg
    12541 tgatttctgg gtaagtcttc ttaatatgtt tcacttgatc gataacacct ttagagtgac
    12601 catgtgctgt atcgataact aagacatcca cacctgcttc gactaatttt tgagcacgaa
    12661 tatcagtgtc ttttgaaata ccgattgctg cggctacaag tagacgacca tgttcatcct
    12721 ttgctgcatt agggaattcg atcacttttt caatatcttt aatagtaata agaccttcta
    12781 gacgtccgtc tttaactaat ggtaactttt caatcttatg tttttggaga attttttctg
    12841 cttcttcaag tgttgtattc actggagctg taattaaatt ttcttgtgtc attacatcta
    12901 caattttaat tgagaagtct tcaataaagc gtaagtcgcg gtttgttaaa atacctacta
    12961 agttgcgatc ttctttatta tcaacaattg gtacacctga aatacggtat ttacccatta
    13021 atgcttctgc ttcataaacg ctttcttctg gcgttaagaa aaatgggttt gaaatgacac
    13081 cattttctga gcgttttact ttttgaactt cgtccgcttg ttcttcaacg cccatatttt
    13141 tatgaataac acctaaacca ccttgacgag ccatagcaat cgccatttta gattcagtta
    13201 ctgtatccat accagcagaa ataactggaa tattcaattt aactttgtct gataattgta
    13261 cgcttaaatc aacgtctttc ggtaaaatat cagattgtgc tggaattaat aacacatcat
    13321 caaacgttaa tgattctttt gcaaatttac tttcccacat taaaaacagc ctccattttt
    13381 caaattaatt agttatatta tttcacattt tcttccgttt gtttatactt tattccatta
    13441 aaaaagaaat taagaataat tgcagagatt gcgcctaaaa caattccgtt ttgagtcaac
    13501 catgcaaatt gttcacctaa acctttaaat gcttgtggta cagcgcttat accagtacct
    13561 aatcctactg atacagcgat aattaataaa ttgttttgat ttttaaaatc gatatgtcct
    13621 aatatactaa caccatatgc cattaccatg ccaaacatag ctatcatcgc accacctaac
    13681 acaggtagcg gtatgatatt tgctaatgca ccaagcttag gtatacaacc acatataagt
    13741 aataacacga ccatgccgta tataacatta tttttcttag cgccggataa agaaacaagt
    13801 cctacatttt gcgaataggc tgtatacgga aatgaattga atatagaacc taacactatc
    13861 gctagacctt ccgcagtata acctttacga aaatcttttc tttctaactt cttaccggta
    13921 atttcactta acgcatgata gacacctgtc gactcaatta aactaacgat agctacaata
    13981 aagaacacta acgtcgatgt cacatcaaag ctaaatccag agaatctaaa cggcactggg
    14041 atgcctaacc aaccggcatg attgacttga ttaatatcga ccatcccaag taagccagca
    14101 cctatcgttc ctaaaacgag tccaattaat atggcaatac tcttaataaa tccagttgtg
    14161 aatctttgta aaagaagaat aatgattaat gtcattaaac ctaacaaaat gttcttaaca
    14221 tctccatagt cctttgcacc ttgacctcca gctaagtaat tcattgctac tggcattaaa
    14281 ttgataccaa tgatagtaac aacactaccc gttactactg gtgggaagaa ttttacaaga
    14341 tgtgaaaaga aaggcgcgat gataataact aatatccctg ataaaaatag cgaaccgtaa
    14401 agtacatcta ttcctttcgt ttgaccaatt aaaatcatgg gcgcaacagc cgtgaatgta
    14461 catccaagaa cgattggtaa tcctgttcct gttactttat tggcttgtaa aaatgtggca
    14521 actccacaca taaatatatc tactgtaact aagtaagcga tttgttcagg tgtaaacttc
    14581 aaacttgtac caacaatgat tggaactaag atagcacctg cgtacatagc taaaagatgt
    14641 tgaacactta ggattaaatt tttcattatt cttctcccac caatgtcact ttgtttcctt
    14701 ctagtgaagc aaccttgcag agagaagaaa ctgttaaacc tgcttcttct aaacgtttat
    14761 gcccattttg gaaactcttt tcaacaacaa taccaatacc agctgtctta gcattcgctt
    14821 gctgtgcgat atcgtataat cctaatgaag catcaccatt tgctaaaaag tcatcgatga
    14881 tgagtacagt atcttcttct gataaaaatt cttttgaaac aatgaccgta cttgttttat
    14941 ttttagtaaa tgaatgaata gatgtttcat aataaccatc cgtcaaagtg ctaggttttg
    15001 cttttttcgc aaataaacat ggcacatcaa aatgcagtgc agccatgatt gcaggtgcga
    15061 taccggaagc ttcaatggtt aagattttag taatcccttt atctttaaat tgctcgtaaa
    15121 aagtgcgacc aacttcattc attaactttg catcaatttg atgatttaaa aatccatcga
    15181 cttttaaaat cttctcatca atgacaacgc cgtcttcctt tactttttgt cctagtaact
    15241 ccactcttaa aacctcctca gatttgtgtg aaacattttg ctcttaaatt ggtgctagat
    15301 acaaaaaaat ccccaaacta aataatagtt tcagggttta tgagtgaacg aacatgcata
    15361 acgaatttgt catgcaatca atgtaaaaga agtttcatca aataaactgt gaccatcatg
    15421 taaaatgata taaatcaccc accatggtta caatttaatg gctgaagcta ctcctagtat
    15481 tgtgttgtta ctcatagtca tgtcgttcaa ggcaacatgg tagaaacttc taaagccata
    15541 ttctttagat tatatgagtt tatgtaaatt atttaacgat aatagcaaat tttcggcatt
    15601 ttttcaataa ctgcttaggt aatcttttaa tagttttata aatgtacaga gacgtttaat
    15661 gcgaatgaag acttataact taataatgta agggttttac tttataataa gaagggtaaa
    15721 agattaaata aacaaatttt taaaggggcg acaactatgg cagatattac tgtagtaaat
    15781 gacactggcg aattatacaa cgtaatcaat caaaagaaat ctgaaggtta tttagaatca
    15841 gagctaacaa ttattagtaa aagtaaatta cacctaaatg acttacacga ctctgaaatc
    15901 tcccttatct caacaagtgg aacatttagt gatagaatga ctaaactgtt aacaggagaa
    15961 gatggtgaac atgcagtttt atcacgctac aacttagcgc ctgatgaatt agaaaaatat
    16021 aaacaactta ttttagacga taaaatgtta gttgtagcgg ttcgtgacaa atcatcacat
    16081 caagaagtgc atgaaaataa ttcagcttat gaagagattg atattacaca ttttgctgaa
    16141 gcatctaaag gtcccaaagc ttagttatca cactgaattc aaaatgagat tactgtactg
    16201 tctaatttaa ggtagtacag ttttttatac catttaaaag tttatgaaca gtcttttaca
    16261 gcaggcgatt atgctataat attatttaaa ttggggaggt gttcttatgg aatttaaaac
    16321 cattcaatct gtcaaagatc cacattacaa cgaagcactt aaactatacg atactaagtt
    16381 aaatttatct ttaactgaag ataaacgtat catcaaacgc tcattagaaa acaacaaaac
    16441 cgaaaacgat tatgtcttta tcgtaggctt agaaaacgat gttgttgtca gtctagcaac
    16501 tgcacattat gaagccacaa ccaattcagc atttttaatt tatttattcg caaaagacct
    16561 acccaaccat gatgagttaa tgacgcaaac attacatcaa attgaacatg aactaaactt
    16621 attatcaaac cttgttcatt ctagaagtat taacttttta atgatggaag tccctaaaga
    16681 acctgcagat ttaacacaag ataacggcct cattattgaa caccgtagac agtttttata
    16741 cgaacatcaa tttgaaagac aaactgaaat tgattacatt catccaacta atgatggtca
    16801 cactaaaaaa gtggacttat ttttaaagaa aaatattaaa ctgacaaagg atatctacgg
    16861 tacaagtgtt aaatcaaatt atattttgaa gtatgtattt gccaatggta tcgcacgtga
    16921 aattatttat gctttattag aagaaatgga tttacgtaaa ccattttaaa caaacttgaa
    16981 atcactaaca ataacgttta aactatgtat gaagcaagac tgacgtttca ctgcccgtag
    17041 acaaacgtca gtatacataa agtatatgca gaaaggactt taaagtatgt taactaaaga
    17101 atttgcacaa cgcgtagaac taagtgagaa gcaagtacgt aaaattgttc aacatttaga
    17161 agaacgtggt taccaactaa gcaagactga atatcgtggt cgtgaagcaa ctgatttcaa
    17221 agaagaagat atcgagcttt tcaaagacat tgccgacaaa gtaaaacaaa caaatagtta
    17281 tgatctagcg tttgatgaat tagaaaaaga aaaagacttc ctgcaagtca ttgtcaaaaa
    17341 cgatgacaaa aacttaccta ctaatcaaaa tgtcgctcaa ttagtagaag atttacgcct
    17401 agaaatccag aaaatgcgcg aagaacgtca cctacttggt caaatgatga atcaagtaca
    17461 tcagcaacaa caagaattaa aagaacttca aaatcaactt acatctaaaa tcgattcaaa
    17521 tagcgaatcc ttaaaagcta tccaaacatc acaagaggct atccaagaag cacaagcatc
    17581 tcaagctaaa gtattggctg aatcaacaaa taaagttgaa aaaaatgctg ttactgaaga
    17641 taaagctgac agtaaagatt cgaaggttgc tggagtgaat acgtcaacag acgctaagac
    17701 ggatactaaa gcagacaatg caggcgatgg cactactact aaagtagata aagaggatca
    17761 aatttctgca actgaagcaa ttgaaaaagc cagcgttgag cagtctaaaa atggcaatgc
    17821 agctgagact tcaaataaag aagctacagt agatgcagaa gcgcaacatg atgctgaaca
    17881 acaagtagca gaagcccatg cagaagcttc taaacaagct acttcgaacg attcatcgga
    17941 agcgaaagta gaaaataaca gcacagcttc acagtctgaa atggcagaac ctaaacctca
    18001 agaagagaaa aaaggtttct tcgcacgttt attcaacctg tagggagtgg gacagaaatg
    18061 atattttcgc aaaatttatt tcgtcgtccc accccaactt gcattgtctg tagaaatcgg
    18121 gaatccaatt tctctttgtt ggggccccgc cggcaaggtt gactagaatt gaaaaaagct
    18181 tgttacaagc gcattttcgt tcagtcaact actgccaata taacttcgta gagcatagaa
    18241 cattgattta tgtcccagcc tgtaagactc agtaatatat atctatgatt taaaaactga
    18301 ttttttataa aactatagaa ctgtaattta ttttcaaatt taatactcaa ttcataaaac
    18361 ctgaaacaag ctgatatact ccattcaata tctactttga agagagcata tcaaactaat
    18421 ttgtttcagg ttttttaatt attcattatg attaatattg aattttgaat aaatttttat
    18481 agtatgttac ttttaaatgg gatggctcct gtgtattcct aaatagattc atcttaataa
    18541 gcccattcaa tttaaatgtt tggctgtgtt agtaactgac gaatcgcata agggcacact
    18601 catattcaca tttactacaa acgccttttt gctcaaacaa aaatatttgt ttacagtaag
    18661 cttttgaact accacctgca cacacgaaca ctcaaaagat gctaattttg attcgacaga
    18721 tttttttaca acgtgtttgt tcgaagcgtc ttttcaaggt tgatcattta ttatcttttt
    18781 attcaataaa tatgaggtta gcaattttta tcaaaccgtc atagccacat ctcgattcag
    18841 gaatacattt gttttctcat atcttatctc tcatgacagt acacgatata tatcttttcc
    18901 aaatgttcat cacttttttc ctgttaaaat catttctatt attaacgctg taatgacaac
    18961 ttatttaatt tattgcattt aatgccctgc ttcattttca aatatcaact ttaatcgact
    19021 aggtcataac actattaacc cttttcacct ttctctacta aattcaaaac gatttgttta
    19081 caaattccct tatgccaacg gtcgaatttc atcatgttta catttaattg caaatgccct
    19141 cttgaaatat tttaaaatta atattattct tagcatacta gtcggaatta tattcaaaaa
    19201 tttgaggagt gacgttcatg aatgcttttc ttacactgat taatattatc gtattggtga
    19261 ttttcatcgt aatattgcac atgatggctc gcaaacatat atcttttgcg aaacgtgtgt
    19321 ttacagcttt aggtatcggc attgtatttg gcgtgttatt acatcttgtc tatggaacac
    19381 attcgaatgt tattacatca acaagtgact ggttcaacat tgtcggacaa ggttacgttg
    19441 cattgttgca aatgattgtg atgcctttaa ttttcatttc tattgtcgct gcattcacta
    19501 aaattcaaat cggagaaaag tttgctaaaa ttggtagtct catttttatc tttttaattg
    19561 gcactgtcac cattgctgca attgttggtg ttgtgtatgc cttggtgttt ggtttagatg
    19621 catcaacaat taatcttggt aacgctgaac aagcacgtgg aagcgaaatt gctaaacaag
    19681 cgaaagattt aacagcacat acgttaccac aacaaatttt ggaattattg cctaaaaatc
    19741 cattcttaga tttcactgga caacgtgcaa cttccacgat tgctgtcgtg atatttgctt
    19801 cgtttatagg atttgcttat ttacgcgtag cacgcaaaca acctgatcat ggtgaattgc
    19861 ttaagagtgc gattgacgct atctattcac tagtaatggc gattgtgacg ttcgtcttac
    19921 gtttaacgcc atacggtgta ttagcaatta tggccaacac gttatcgaca agtgactttg
    19981 gtgccatttg gactttagga aaattcttaa tcgcatcgta cgctgcatta attacgacgt
    20041 atatcattca cttagttatt ttaagtctgt taggtattag cccaattcgc tatgttaaaa
    20101 agactttgga agtattaatc tttgcattca catcacgctc aagcgcgggt gccttaccgc
    20161 taaatgtaca aacacaaaca agacgtctag gcgtgccgga gggtattgcc aatttcgctg
    20221 caacatttgg attgtcgatt ggacaaaatg gttgtgcagg tatttaccct gcaatgcttg
    20281 caattatggt cgcacccgtc gctaatgtag aaattgattt acaatttatt gtaacgttaa
    20341 tcgctgtcgt gattattagt tcattcggcg ttgctggcgt cgggggcgga gctacatttg
    20401 catccatatt ggtgttgtca acgttaaact tgccagtcgc tttagccggc gtgctcatct
    20461 ccgttgaacc attgattgac atgggtagaa cagcattaaa cgtgaacgat tcaatgctag
    20521 ctggtacagg taccgccaag ttaactaaac attgggataa agatactttc gattcaaatg
    20581 ataatgctgc attaacttca cactaattca ttgcaagaaa tacccgcccc ctctagctat
    20641 acttatcatt ttaagctaga gggggcgggt tattttggta tcttgctatc gctgtattaa
    20701 tcctgatttt tgcaattgtt ctaacatatc taatcttgct ttgtttccta ggaacatctc
    20761 aatttgctgc gaccatgttt ctttgcgatt cccgtttgta cgttgatcat agtactcgct
    20821 gattgtctgg tcgtaatctg ccatttgtgc atactgtgtt tccttatcag catgatactt
    20881 attatgatgg aagacatggt caaatggtaa gcgtggcttg gctgccccat tttcatcatc
    20941 tgcgggttcc cctactgcca taccaaatac cgggaagaca tagtcaggta agtctaaaat
    21001 ttctcgaact cgttcaacat catttcttaa tgatcctaaa aagacaatgc cataccccat
    21061 atcttcagca gttaccgcaa tattttcggc aactaatgct gcatcgattg cacctactag
    21121 caaaccttcc gttgaaccat atgcattttc catatcagtt tcagcatgtt gatcaactaa
    21181 atggtgacga taataatcaa taacaaagac gaataaatag ccattttcta caacataagg
    21241 ttgtccagaa acttctcgta aattttcttt aatcttctca tcgtcgatac caataattga
    21301 gtatgcttgc aggaaacttg acgtcgaagc gctttgtcca gcttctacca atttcttaac
    21361 aacgtcttca cttaaaggtt tattcttaaa ttttctaaca gaatgatgct ttttcacaag
    21421 attatataca tgttctgaca cgttacccac tcctcgatac tttacagtta attaaaaatc
    21481 ttcatattgc aattgtacac gtacaaactt ttttatagca attatttgct tataagttaa
    21541 taattgaact tttaaaaata ttgtctcgca cttgaaaaat ccagtttctc atgcatcacc
    21601 ttcatccatc tcgaacaaat attctcacac cattatcaat aaggttgact tagttgagaa
    21661 tacaactctt ctttttttac attttttaca ttaaaatcaa gatattaaat atagacacaa
    21721 ccttttaaat catcaactac aaaatccatt atcaaagcac tttatattat ctttctctat
    21781 tttaaaagta atcattcact ttacaaagtt atccagttga ctattctgaa tcaaaatgta
    21841 ataatgctaa ctaagagatt agaattatta taattaagca caatcaattg atagttctaa
    21901 ttcatactta aattcttagg aggaagatat ttatgtcatt aattaacaaa gaaatcttac
    21961 catttacagc gcaagctttc gatccaaaaa aagatcaatt taaagaagtt acacaagaag
    22021 atttaaaagg ttcttggagc gtagtatgct tctatcctgc tgacttctca ttcgtttgtc
    22081 caactgaatt agaagactta caaaaccaat atgaagaatt acaaaaatta ggcgtaaatg
    22141 tattctcagt atcaactgat actcacttcg tacacaaagc atggcatgac cattcagatg
    22201 caattagcaa aatcacttac actatgattg gtgacccatc acaaacaatc actcgtaatt
    22261 ttgatgtatt agatgaagct actggtttag ctcaacgtgg tacattcatt atcgacccag
    22321 acggtgttgt acaagcatct gaaattaacg ctgacggaat tggccgtgac gctagtacat
    22381 tagctcacaa aatcaaagca gctcaatatg ttcgtaaaaa ccctggcgaa gtatgcccag
    22441 ctaaatggga agaaggcgct aaaacattgc aacctggttt agatttagta ggtaaaatct
    22501 aaggaggcat tacaaccatg cttaatgctg atttaaaaca acaacttaaa caattattag
    22561 aactaatgga gggcaacgtt gaattcgttg ccagccttgg ttcagatgaa aaatctaagg
    22621 aactcaaaga attattaaca gaaatttctg atatgtcacc tagactttca ctttctgaaa
    22681 agtctttaaa acgtacacca agtttctcag tcaaccgtcc tggcgaagaa acaggtgtaa
    22741 catttgcagg aattccatta ggtcacgagt ttaactcact tgttttagca attttacagg
    22801 ttagtggtcg tgcacctaaa gaaaaacagt caattattga ccaaattaaa aacttagaag
    22861 gttcattcca ttttgaaaca ttcattagtt taacgtgtca aaaatgtcct gatgtcgttc
    22921 aagcacttaa cttaatgagt gtgatcaacc ctaacatcac gcattctatg attgatggtg
    22981 cagtgttccg tgaagaatct gaaaacatca tggcagtccc tgctgtcttt ttaaacggcg
    23041 aagaatttgg caatggtcgt atgacaatcc aagatattct ttcgaaacta ggcagtacgg
    23101 cagatgcatc tgagtttgaa aataaagaac cttatgatgt cttaatcgtt ggtggtggtc
    23161 ctgctagtgg tagtgcagcg atttacacag cacgtaaagg tttacgtact ggtatagttg
    23221 ctgatcgtat cggtggccaa gttaatgata ctgctggtat tgagaacttc attactgtta
    23281 aagaaacaac tggttctgaa ttttcttcta acttagcagc gcacattgat caatatgaca
    23341 ttgatgcaat gacaggtata cgtgctacag atatcgaaaa gactgacgaa gcaattaaag
    23401 ttacgttaga aaacggtgct gtcttagaaa gtaaaacagt cattattgct actggtgcag
    23461 gttggcgtaa gctaaacatt ccaggtgaag agcaattgat taataaaggt gttgcattct
    23521 gccctcactg tgacggacct ctatttgaaa ataaagacgt agcagttatc ggtggcggta
    23581 actctggggt tgaagcagca attgaccttg ctggtatcgt taatcatgtt acattattcg
    23641 aattcgctag cgaattaaaa gcagacaacg tgttacaaga tcgtttacgt tctttatcaa
    23701 atgttgatat caaaacaaat gccaaaacta ctgaagttgt cggagaagac catgttacag
    23761 gtatacgtta cgaagacatg agcaccggcg aagaacatct acttaactta gatggtatct
    23821 ttgttcaaat tggtttactt ccaaacacat catggttaaa agatgctgtt gaattaaacg
    23881 aacgtggtga aattgttatt gatcgtaaca ataatactaa cgtacctgga atatttgctg
    23941 ctggcgatgt cacagatcag aagaacaaac aaattattat ttcaatgggc gctggtgcaa
    24001 atgcagcatt aaatgccttt gactatatta tcagaaacta aagtttcatt catttcactg
    24061 acagtatctt acatttgtta agatgctgtt atttttttat cccgacataa aatcatgtta
    24121 tgataaactt aatatataca acgaggtgtg caaaaatgct tgatgttcat tttatatacc
    24181 caagaaaatt acaaaacgca tgcgaatatt ttaatttacc gacccaaaaa gaaggatttg
    24241 aagtcacgat gcatgcttta cgccctgaaa atatacataa aatttatcat gatatggata
    24301 agacagaaca aaaatttata gaaacactta tatctaatgg taatcctgtc acaattataa
    24361 ataaacgcga tgttaatatt ttaaagctaa caaatcatct aggttttgtt tatcaactta
    24421 acgaagatga gttctttgta aataatttct ttttaagata tatcaattca gaggtactag
    24481 ataatgcata tggtgaattc gaatcgttag atacaataaa ccatatgaat ttaaatgaag
    24541 aagtgttacg tgtaagacaa catacgcgct taaacgatgt cacattagaa catgctttac
    24601 tgttaaaaac taatgacgag ctaaaaacaa tatgcaggca gtatggtatc aaaggattct
    24661 caaataaaaa caaacaaaat cttgttgaat taattatgac agcatttttc aataataatc
    24721 acatcattga acaagtgttt tctaatacta ctctatttga gtttgacatt ctttctagca
    24781 tacttgaaag tgatgctaac taccgattaa acaatgaact aattgaattt gaaaagctac
    24841 aattaataaa ttttaacaac tttttaacta gtcattttta ttattattac gactttgaat
    24901 atgattgtct tattttgcca aatgatgtgc gtaaacatat tgaaaatttc gtgaatcaag
    24961 tcggcaatgg tgattttgag aaagctgtaa atcagtttgt tgatgagcaa acaattgaga
    25021 aaagtttagc agagtttgaa aacgcgttag agttatcatt tatggacgac gaagttgact
    25081 tagatatgtt acttaaagat gatgactttg aagattttgg tacaaccgat agcttaagaa
    25141 gtgatgcaat cactcaattg atttctgaga ttaagaaagg attaatagat aaaaaagata
    25201 ttttacctct tcgtattata cttggttctg taaacttgta tggtatttta tcaactaatc
    25261 atctactata tctcacacaa agattttatg acgctacttt tagtaaagaa gagttgcaat
    25321 atagcttaga tactttagct ggtactgagc tatattatat tgaagatgac ttcgtgtttc
    25381 accctgtgtt atttgatgtt gtagagtctg aggcgtcagg cattgatatt tctgatgaag
    25441 gatactatgt accagcttct gttgaggaat taatctatta tgatagacac cactatttga
    25501 aatctgattc agaaattaaa gcgttcacaa gttatctacg caaacaaatc aatatgaaaa
    25561 atgatattga gaaggaaaag tatgtgaacg aaatcattat gcttttacgt acgataccta
    25621 cctctaaatt gatacaacca attatgcagt tatttataga taatgctatc cttaacaatg
    25681 ttcaaaacga cgcattattg atagaaaaag taactcatgc acgtaatcat ttacgtctat
    25741 ggtcattgca aggtcatcgt gacattccaa actaattcaa tgttactcta ttaaacatga
    25801 atacatattt tttcaattaa agataaacta aggagttata ttacattatg aaaaagagat
    25861 tactactaag tacattttta gcatcgacat taattcttac aggttgcgcg tccgatcaat
    25921 ctgataacga agatcatcat acaagtactg gcatccatgc acctaaaagc gctaaaaaat
    25981 tagaaacaaa agatatcttt aactcagata agaaaaatag tgatattagt gatgcagaga
    26041 tgaagcaagc tatagaaaag tatttgtctg taaatagcga tatacttgat aacaaatata
    26101 ttatgcaaca taaactagat aaacaaattg acagtcaaac aaaagtgact gaaaaacaag
    26161 cagaaacact tagccactta tctaacttag ctgttaaaaa cgatttgcat ttcaagaaat
    26221 ttgtcactga aaacaatatt cctaaagaat ataagaaacc cgttgaatta atgatgaatt
    26281 acttcaaagc tttaaatagt acaatcgcca atgttgatga agatattgaa aagcttagct
    26341 atcaaccgca aaataaaatc aatgttgtcg acgtgccaac aaaatatgcc ggtgatgtta
    26401 ataaaaagca acaagataaa attaaagatt tcttaaaatc taaaggtatc aagagtgatg
    26461 ttatcgataa ataactgaaa gccatcattg cgacatgtaa cacatcatta catgtgcttt
    26521 aattcatgac aattctttat ctttaaaagt gactaatcaa tttagctaaa gtcatcattt
    26581 gggcatcaag cagcatatta ctgtttgatg cttctttaat tgccttaaat tctctatcta
    26641 tgccaaaatc acccattagt tgacgcattt gactatatcc ctaacatatt ccatacttat
    26701 attgactcta taaacaaagg atgatcatga tgaaagttcg aattactttt attattttag
    26761 caattttatc gacgattgtt tgcttgttct tagcagccat gcatccaaca ggaccaaaca
    26821 cagtaacttt tgaacagcca tatttattca ccctcaacat cattataatg gtattagtcg
    26881 cattaccttc acttgtttaa gctatatatg attatatgag ttttagaatt tcttctacta
    26941 ttttacaatt tctaggggct gtctcttggt tctttttatc attgatatta tcgctcacac
    27001 aatttacacc ttttacatta gcgtcattta taacttcaat tattttgttc gcaagcacaa
    27061 ttatcacatt agctattggt ggtaagtctg ttgataagaa tgattcccat taaattccaa
    27121 atacaaaaaa ggttctgaag gccgctataa aacacagctt ttcagaacct caatacttct
    27181 attcaatgat atatggtttg caattttcaa cttttaaatc cacagcttct tcccgtgaaa
    27241 cgttattaaa ataaaccatt aaacaacgaa tgacaacttg atgtgcaaca atgacaatat
    27301 catctttttg tgtatcttca ttgacaacat gattcataaa atgttctacg cgttgatata
    27361 catcttcata actttctcct tcaggcgctt tttgtgaaaa actatgacga aagtctttaa
    27421 agtttgaatc attgaaatat ttttcatatt tcggattcgc actgatttca tctttatatt
    27481 caccctcaaa tacgccaagt gaacgttctc ttaatagagg cgtagtcgtt gatgcaatgt
    27541 catatggaaa aatatgttca aacgtttgct gtgttcttaa taagtctgaa acatatacat
    27601 gtttaatctg tttctcttta aaataatcac ataaatcgtc cgcacttttt gtgccagtat
    27661 ccgttaacgg cacatctaat tgtccacaaa aataagatct aaaatgttta ttatcataat
    27721 tcgattttga ttcgccatgt ctaactaaat aaatcgtcat aatattactc ctcaccttat
    27781 gtatttcata tctaccataa cactttgact actaattcga tatcaatctt aatattctat
    27841 tctaaaaaaa taattaattc atattttaaa ttaatgactc taaaaatagt attgtcttta
    27901 atcaagtgat gtgttactat atgggtgtag ttcttaaatg actacacagt cttttatatt
    27961 catgaacata atactcctgg atgacgcaac ccaacgtcat ccttttttta tttatcaaat
    28021 acttgctttt ttatttccaa cctctcctca tgttaattta ttacttttta atcctatctt
    28081 acctacattt tcaaatatta taatacatat tgaccatttt cacgcaaata aaaaagatga
    28141 caactgaatg ccatctttat attaattcaa tttccaattg aattttttat tatttacgca
    28201 tagctcttaa aattaacgtt acgattgcaa ttaagataat tgaaccaatt aatgctggta
    28261 agatgtaaat tcttcctatt tcaggacccc attgccctaa taacgttcca cctaaccatg
    28321 atccaataat acctgcgata atattaccta aaataccacc tgggatatct ttacccataa
    28381 tagcaccagc agcccatcca attaagccac cgacaattaa cattccaata aatccaaaca
    28441 taattttcag tctccttttt ctatttattt tgcgttattc taagtagtac cccttattta
    28501 caattctaaa acaaattcaa attattttta tccaaatatt tttaaaagta gtaattgaat
    28561 atcaatttta ttcaatgtag ctatcgttat ttaaagtctc tgtactggca atatcatata
    28621 catttacatt attttttctg ccgaattcat agcttgatta ttttatgtta taggactaga
    28681 atatacacat attattagag catctttgaa ttttaaatca agaagcgagg ttaatgaaca
    28741 atgaatatgc atattttata taacttacga actaaacata atttagaaat tgacgaatta
    28801 gcacagcaat taaatgagaa atatggtact aaatatgaag cacatcaaat ttgggaatgg
    28861 gagaatcatc accatgaacc taaatttaaa gatgccatgc atttagctga cttctttgat
    28921 gcaccatatg aaatgttttt agaaagtaag gttaaagaat atcagaaaca tttagaagaa
    28981 gtcgatattc gcatggataa atagatgcaa ataaaccctc acaacacgtt tggcatatat
    29041 cctttcaaat ctatactgga tatattacat tacgttgtga gggtatttta ataattaata
    29101 tgaattaaga cctgttatta tcattagtat tacgacatat tttagtgatc ttgctcactc
    29161 tttaatactt taccgttctt agcatcaaca gtaacttctt gttttttatt accttttttc
    29221 aaatcgatat tgtaaacaag tttgccatca tctttttcaa gtgaccattc tttaatatca
    29281 ccatcaaatt ctttttgtcc ttctttaatg gcttttttgt aatctatagc atcgctatat
    29341 ttaaagttat cattttcatt cactgtatct tctttttcag tctttttatt aatcactttt
    29401 ttatttttat cagcaacaag tacttctgac tcttcaccag atttttgttg cgtcacttta
    29461 taagcccatt caccattaga attttcaaat gaaattcctt tcaacttttg gcctttgtaa
    29521 gtttcttcag cttttttcac agcatcttct gggcttgttt taacatcttt taaagcaata
    29581 acatcttttg ttgtattagt gtcttgatta gtatttgact ctgttgattt tgtttcatct
    29641 tttggagtat cattgccaca tgcagtaagc accaccgctg acattgataa cactgctaat
    29701 gattttaatt tcataatatc actctctctt cctatttttg aaactcataa caaaagctta
    29761 tatgctatat agattgtatt accccttgtt tttaatttta ttcataatta ttacaaatat
    29821 ttttaaatta atcatcatgt gttactttcg ttcgtactca ttttataatt gaaccatgta
    29881 atataagcat gctattatcg actcagtttg tctaatgctt ttttaggtac ttcttccttt
    29941 tcaacttctt caaaagtttc tacatgatga cctttatgtg taattttcaa gtatctatgc
    30001 ggtttaacat caaatgtagc agtatacatt aattgcgttt ctttcccttc tttattaaaa
    30061 gcactttgag tataacttcg gagttgatca tccattctag tagacacctc tgtcgtttta
    30121 acataagaat catcttcctt tactaatggg ttaaattgat ctgttatacc atgagagtct
    30181 attgttttta aaatgaataa agcagcataa gcgccaatga tagtaagtgc aagatatgtt
    30241 attgttttta aaatcgtttt caaaagatca cctctgacaa aatataaaat aggatgcctc
    30301 tgaaaacaac ttacataatt gtaattaaag tgtaagtatt aggagaaaat gatggtattt
    30361 gaatctgaaa gatacgaatc gtttttaaac taagttattt tatagagact ttattatgga
    30421 attgaaagaa gttaatttta aggttgtcga atataaaaac atattgatta accataaaag
    30481 agaaattatt tttgtagatt tcggcataaa catcattaaa gaattttcta tatgatggtg
    30541 ctaatcataa caaagaaagt taggcatctt cggataccta actttcaaag tattagcgat
    30601 aaaacactat atatactaat ttaacttaat ttcaaacttg ttcaatgtca ttaacacttt
    30661 tcacaagttc gaaagttaca ttttttatat catcagtatt aatttctaca ttttccctat
    30721 tcttttgtaa ctctttatgc gactctaatg tatatttacc accatctttt gtatttatga
    30781 cgatgttacc tttaggcaaa tgttctccca tatacaagcc ataggaaata tgagcaaatc
    30841 ttatgatatg atccaattct tttaaagtga caacttcttt attaagagta atgttcctct
    30901 taggtgtata cactctgcca gcaactgtgt atgtaccttc taaatgtata cgaacttggt
    30961 tttcatttaa aggtctctct gaataaaccc aattcctaga tttattagat tttccattta
    31021 ttgtatcctt gctgtatcta tcgtaaagtt ttttaaccaa aactttttct gaattatttg
    31081 gtacttcttc aactttttgt aattgtttat cttgtacact tgaggaattt tgtgttgacg
    31141 cgtctgcttt tggatatatt ccaaatgttc ccccatagat gatacctagc gatagaatag
    31201 attttaatac gattgaattt tttgagtcat attttttgaa catatttaat tacctccttg
    31261 atgtaaagct tttatttgct acaattataa aaataataga cgtgttcatg aattaaattc
    31321 atcttaactc ttgattaact ttaattttct atcactttgt ctttaataac tataaatcgt
    31381 ctacacataa ttggacaaaa tctatgcgaa tagaatttgt taatttaaaa tagtaagcaa
    31441 ttcaaagtta tatgtgtaat agataaaata gatatcccta tagtgatgcg ttactagcta
    31501 aacgtaataa cactttagaa gataatgaag ataaggagtt actggattgt ttcgactatg
    31561 taattaagta taaaaatatc caacgacaaa acgtaattat aaaatggtaa aagctatggt
    31621 acagtttcaa attgctaatg acatgcgtat cggtgagcta cttgcaataa agagagaaaa
    31681 tataaactat gaagataaaa cgctagatat cgatggtaca attaattgga taactgaaaa
    31741 aagacgggag cattcggagt aaaggagaca actgaaagaa gtaatagcta taaggccaca
    31801 gggctcacta cccaaagcat cgacttacta agaacactta tgcttggaat gataagttta
    31861 ttgatagaga gtacatattc acaaatacgt ctggtagccc tatcgactcg aacaaaatta
    31921 gccacattat taaagggggg cgctgatatt agttctatta agaaacctat aacgacgcat
    31981 acattacatc attcgcatat atctacactt gctcaattag gaattaactt aaaagcaatg
    32041 caagagcatg taggtcattc agattataaa aaaaatctag agatatacac acatgttact
    32101 aatcagatgg cgaaagatat gatgaataaa tttgaacgat tggggagtta aaattggaaa
    32161 aagatgatac actagcagaa attaagccta tgctcaattt tgatgagcaa atagcgaaat
    32221 taaaacagat gaatatattt tttaatatta ttgacaccga aaaagcaaat gaaattctta
    32281 gaaaaaataa ttacttcttc aaactagctt atttccgaaa aaatttcgaa aaaaagaatg
    32341 gcggctattt catagaattt gcttatttat cagatttagc aactatagat atgaaattaa
    32401 gatacacaat gttgcattta actttagata ttgaacatag tttaaagtgt ctagtcttaa
    32461 aactaataac agaaaataac caagaagatg gttataaaat aatagatgag ttcttatgta
    32521 ttgataaatc atatagcaat tcaaattttg acacaaattc aagaacacca gaagaagtta
    32581 tggaaaccaa aatcaaaaat aaaaacgaaa tattcaagca tatgaataaa cgaggacaac
    32641 tacccgagaa gttgaataaa tactatcaaa atccacccgc atgggtttgc attgaattca
    32701 tgcaactagg tcaattcgtt tcgtttctca acttctatta caagaagtac aatgacgaag
    32761 aattgagagt tgctaatatt ttaatgcctt tagttaaaaa tataagaaac aaatcagctc
    32821 ataaccaacc catcatagca aatctaaatt atgacagtag atctcctcaa tatttatttg
    32881 aaaaagggaa taatataggc atatctagaa acatgttcgg aataaaaaat ttcatagata
    32941 ctttcgctac gctagaatta cataatcaag tttgtagtaa tgcaattatc caagcaagat
    33001 atcacgattt ggaccaactt caaaagcgat ataaaagaaa cgaaagctat tataataatg
    33061 cattagctat caaaagattt tttatagctt tagataaaat tattgacttc aacagaccaa
    33121 aagtataaac tatctagtga ggaaagagac ttataggtct cgcgagttat tttaattcgt
    33181 atgcaagaaa aagaagagct atgcatttta tttaaaatgc gtagttcttt ttttatgcat
    33241 ctaaattcat attttt
//
LOCUS       DAHHYJ010000013        32289 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000013 DAHHYJ010000000
VERSION     DAHHYJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.013"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            60..6869
                     /gene="sasA"
                     /locus_tag="NBK22_000473"
     CDS             60..6869
                     /gene="sasA"
                     /locus_tag="NBK22_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
                     /protein_id="HCD2413681.1"
                     /translation="MSKRQKAFHDSLANEKTRVRLYKSGKNWVKSGIKEIEMFKIMGL
                     PFISHSLVSQDNQSISKKMTGYGLKTTAVIGGAFTVNMLHDQQAFAASDAPLTSELNT
                     QSETVGNQNSTTIEASTSTADSTSVTKNSSSVQTSNSDTVSSEKSEKVTSTTNSTSNQ
                     QEKLTSTSESTSSKNTTSSSDTKSVASTSSTEQPINTSTNQSTASNNTSQSTTPSSVN
                     LNKTSTTSTSTAPVKLRTFSRLAMSTFASAATTTAVTANTITVNKDNLKQYMTTSGNA
                     TYDQSTGIVTLTQDAYSQKGAITLGTRIDSNKSFHFSGKVNLGNKYEGHGNGGDGIGF
                     AFSPGVLGETGLNGAAVGIGGLSNAFGFKLDTYHNTSKPNSAAKANADPSNVAGGGAF
                     GAFVTTDSYGVATTYTSSSTADNAAKLNVQPTNNTFQDFDINYNGDTKVMTVKYAGQT
                     WTRNISDWIAKSGTTNFSLSMTASTGGATNLQQVQFGTFEYTESAVTQVRYVDVTTGK
                     DIIPPKTYSGNVDQVVTIDNQQSALTAKGYNYTSVDSSYASTYNDTNKTVKMTNAGQS
                     VTYYFTDVKAPTVTVGNQTIEVGKTMNPIVLTTTDNGTGTVTNTVTGLPSGLSYDSAT
                     NSIIGTPTKIGQSTVTVVSTDQANNKSTTTFTINVVDTTAPTVTPIGDQSSEVYSPIS
                     PIKIATQDNSGNAVTNTVTGLPSGLTFDSTNNTISGTPTNIGTSTISIVSTDASGNKT
                     TTTFKYEVTRNSMSDSVSTSGSTQQSQSVSTSKADSQSASTSTSGSIVVSTSASTSKS
                     TSVSLSDSVSASKSLSTSESNSVSSSTSTSLVNSQSVSSSMSDSASKSTSLSDSISNS
                     SSTEKSESLSTSTSDSLRTSTSLSDSLSMSTSGSLSKSQSLSTSISGSSSTSASLSDS
                     TSNAISTSTSLSESASTSDSISISNSIANSQSASTSKSDSQSTSISLSTSDSKSMSTS
                     ESLSDSTSTSGSVSGSLSIAASQSVSTSTSDSMSTSEIVSDSISTSGSLSASDSKSMS
                     VSSSMSTSQSGSTSESLSDSQSTSDSDSKSLSQSTSQSGSTSTSTSTSASVRTSESQS
                     TSGSMSASQSDSMSISTSFSDSTSDSKSASTASSESISQSASTSTSGSVSTSTSLSTS
                     NSERTSTSMSDSTSLSTSESDSISESTSTSDSISEAISASESTFISLSESNSTSDSES
                     QSASAFLSESLSESTSESTSESVSSSTSESTSLSDSTSESGSTSTSLSNSTSGSTSIS
                     TSTSISESTSTFKSESVSTSLSMSTSTSLSTSLSDSTSDSKSDSLSTSMSTSDSISTS
                     KSDSISTSTSLSGSTSESESDSTSSSESKSDSTSMSISMSQSTSGSTSTSTSTSLSDS
                     TSTSLSLSASMNQSGVDSNSASQSASNSTSTSTSESDSQSTSSYTSQSTSQSESTSTS
                     TSLSDSTSISKSTSQSGSTSTSASLSGSESESDSQSISTSASESTSESTSTSLSDSTS
                     TSNSGSASTSTSLSNSASASESDSSSTSLSDSTSASMQSSESDSQSTSASLSDSLSTS
                     TSNRMSTIASLSTSVSTSESGSTSESTSESDSTSTSLSDSQSTSRSTSASGSASTSTS
                     TSDSRSTSASTSTSMRTSTSDSQSMSLSTSTSTSMSDSTSLSDSVSDSTSDSTSASTS
                     GSMSVSISLSDSTSTSTSASEVMSASISDSQSMSESVNDSESVSESNSESDSKSMSGS
                     TSVSDSGSLSVSTSLRKSESVSESSSLSGSQSMSDSVSTSDSSSLSVSTSLRSSESVS
                     ESDSLSDSKSTSGSTSTSTSGSLSTSTSLSGSESVSESTSLSDSISMSDSTSTSDSDS
                     LSGSISLSGSTSLSTSDSLSDSKSLSSSQSMSGSESTSTSVSDSQSSSTSNSQFDSMS
                     ISASESDSMSTSDSSSISGSNSTSTSLSTSDSMSGSVSVSTSTSLSDSISGSTSVSDS
                     SSTSTSESLSDSMAQSQSTSTSASGSLSTSISTSMSMSASTSTSQSTSVSTSLSTSDS
                     ISDSTSISISGSQSTVESESTSDSTSISDSESLSTSDSDSTSTSTSDSTSSSTSTSIS
                     ESLSTSGSGSTSVSDSTSMSESDSTSVSMSQSMSGSTHNSTSVSDSESVSTSTSTSLS
                     TSDSTSTSESLSTSMSGSQSISDSTSTSMSGSTSTSESNSMHPSDSMSMHHTHSTSTS
                     RLSSEATTSTSESQSTLSATSEVTKHNGTPAQSEKRLPDTGDSIKQNGLLGGVMTLLV
                     GLGLMKRKKKKDENDQDDSQA"
     gene            6999..8210
                     /gene="secY2"
                     /locus_tag="NBK22_000474"
     CDS             6999..8210
                     /gene="secY2"
                     /locus_tag="NBK22_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCD2413682.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTF
                     DSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            8221..9774
                     /gene="asp1"
                     /locus_tag="NBK22_000475"
     CDS             8221..9774
                     /gene="asp1"
                     /locus_tag="NBK22_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCD2413683.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYQLIVLNHAPNLRTFLHRYDLYETKYSSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQTYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDQGEASYQEYLTINGDCVLHEDLKNGRVTVSKRYQHHYQQTEYNNMAQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKLLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDYYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            9764..11332
                     /gene="asp2"
                     /locus_tag="NBK22_000476"
     CDS             9764..11332
                     /gene="asp2"
                     /locus_tag="NBK22_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCD2413684.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLLQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAFELQYTFRLIQTGADGALIEQIVLT
                     DDMLDSPLEIPAKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNRSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            11310..12269
                     /gene="asp3"
                     /locus_tag="NBK22_000477"
     CDS             11310..12269
                     /gene="asp3"
                     /locus_tag="NBK22_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCD2413685.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLGCKVYRTSHEDDDLKGWLTEHVPGNHELKDT
                     NVEVYFKEEQDKHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            12259..14649
                     /gene="secA2"
                     /locus_tag="NBK22_000478"
     CDS             12259..14649
                     /gene="secA2"
                     /locus_tag="NBK22_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCD2413686.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENRDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            14667..16175
                     /gene="gtfA"
                     /locus_tag="NBK22_000479"
     CDS             14667..16175
                     /gene="gtfA"
                     /locus_tag="NBK22_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCD2413687.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDIERELGHEISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            16168..17526
                     /gene="gtfB"
                     /locus_tag="NBK22_000480"
     CDS             16168..17526
                     /gene="gtfB"
                     /locus_tag="NBK22_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCD2413688.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSKGSDVL
                     VWQEHCNGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            17767..19674
                     /gene="sasF"
                     /locus_tag="NBK22_000481"
     CDS             17767..19674
                     /gene="sasF"
                     /locus_tag="NBK22_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCD2413689.1"
                     /translation="MAKYRGKSFQLYVKLSCSTMMATSIILTNILPYDAQAASEKNTE
                     ISKEILSKQDLLDKVDKAIRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKSSHTEMNGQSDIDSKLDQLLKDLNEVSSNVDRGQQSGEDDLNAMKNDMSQT
                     ATTKHGEKDDKNDEAMVNKALEDLDHLNQQIHKSKDALKDASKDPAVSTTDSNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVDQLKKHFASTGDTSSDDILKE
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYILSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            19842..20402
                     /locus_tag="NBK22_000482"
     CDS             19842..20402
                     /locus_tag="NBK22_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD2413690.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(20669..22528)
                     /locus_tag="NBK22_000483"
     CDS             complement(20669..22528)
                     /locus_tag="NBK22_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCD2413691.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNDEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDINQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            22739..25720
                     /locus_tag="NBK22_000484"
     CDS             22739..25720
                     /locus_tag="NBK22_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD2413692.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFVADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPN
                     SNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFSVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(25832..26770)
                     /gene="manA"
                     /locus_tag="NBK22_000485"
     CDS             complement(25832..26770)
                     /gene="manA"
                     /locus_tag="NBK22_000485"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCD2413693.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIQKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(26784..28736)
                     /locus_tag="NBK22_000486"
     CDS             complement(26784..28736)
                     /locus_tag="NBK22_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCD2413694.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLWFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSDLNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWESLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(28813..30687)
                     /locus_tag="NBK22_000487"
     CDS             complement(28813..30687)
                     /locus_tag="NBK22_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD2413695.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFVRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDSKLISQSCIKQINERLGYDLNEKVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHIPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAIDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELSVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     TTIKVAPLFSEDDRHKIRLVVKQKQNPVQAHHHMDTVNFLVVNTEQKSRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISHKDKAFTKQMMQLIANLDDDSVNHLCSLDDHSLKQQLFEYLQE
                     "
     gene            complement(30927..31385)
                     /locus_tag="NBK22_000488"
     CDS             complement(30927..31385)
                     /locus_tag="NBK22_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413696.1"
                     /translation="MKKLLITIVIIVLIGALGFAIYAAINHSTSSSNNQEQENKATHK
                     KTETEENDQQDDSADKADKNQNNGNNVTNDNQPSSQSNVPNNHSTAPSNTQPAQPKDS
                     NSNKNNTHDGGSQSSTNNQNNQQQNTPPANKSNNTNTSSKQTPQAQSPKN"
     gene            31643..32170
                     /gene="isaB"
                     /locus_tag="NBK22_000489"
     CDS             31643..32170
                     /gene="isaB"
                     /locus_tag="NBK22_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCD2413697.1"
                     /translation="MNKTSKVCVAATLALGTLIGVTVVENSAPTSKQAQAAITPYYTY
                     NGYIGNNANFILDKNFINAIKYDNVKFNGIKLAKTNTIKKVEKYDQTFKGVSAKGNEA
                     SQLQFVVKNNISLKDIQKAYGKDLKKENGKTKEADSGIFYYQNAKKTLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT        11202 a         5091 c         6034 g         9962 t
ORIGIN      
        1 ttgaatagtg gcatatatta taatctaatg aatgtaaaga taatacaagg agttattaca
       61 tgagtaaaag acagaaagca tttcatgaca gcttagcaaa cgaaaaaaca agagtaagac
      121 tttataaatc tggaaaaaat tgggtaaaat ccggaattaa agaaatagaa atgttcaaaa
      181 ttatggggct accatttatt agtcatagtt tagtgagtca agataatcaa agcattagta
      241 aaaaaatgac gggatacgga ctgaaaacta cggcggttat tggtggtgca ttcacggtaa
      301 atatgttgca tgaccagcaa gcttttgcgg cttctgatgc accattaact tctgaattaa
      361 acacacaaag tgaaacagta ggtaatcaaa actcaacgac aatcgaagca tcaacatcaa
      421 cagccgattc cacaagtgta acgaaaaata gtagttcggt acaaacatca aatagtgaca
      481 cagtctcaag tgaaaagtct gaaaaggtca cttcgacaac taatagtaca agcaatcaac
      541 aagagaaatt gacatctaca tcagaatcaa catcctcaaa gaatactaca tcaagttctg
      601 atactaaatc tgtagcttca acttcaagta cagaacaacc aattaataca tcaacaaatc
      661 aaagtactgc atcaaataac acttcacaaa gcacaacgcc atcttcggtc aacttaaaca
      721 aaactagcac aacgtcaact agcaccgcac cagtaaaact tcgaactttc agtcgcttag
      781 ctatgtcaac atttgcgtca gcagcgacga caaccgcagt aactgctaat acaattacag
      841 ttaataaaga taacttaaaa caatatatga caacgtcagg taatgctacc tatgatcaaa
      901 gtaccggtat tgtgacgtta acacaggatg catacagcca aaaaggtgct attacattag
      961 gaacacgtat tgactctaat aagagttttc atttttctgg aaaagtaaat ttaggtaaca
     1021 aatatgaagg gcatggaaat ggtggagatg gtatcggttt tgccttttca ccaggtgtat
     1081 taggtgaaac agggttaaac ggtgccgcag taggtattgg tggcttaagt aacgcatttg
     1141 gcttcaaatt ggatacgtat cacaatacat ctaaaccaaa ttcagctgca aaggcgaatg
     1201 ctgacccatc taatgtagct ggtggaggtg cgtttggtgc atttgtaaca acagatagtt
     1261 atggtgttgc gacaacgtat acatcaagtt caacagctga taatgctgcg aagttaaatg
     1321 ttcaacctac aaataacacg ttccaagatt ttgatattaa ctataatggt gatacaaagg
     1381 ttatgactgt caaatatgca ggtcaaacat ggacacgtaa tatttcagat tggattgcga
     1441 aaagtggtac gaccaacttt tcattatcaa tgacagcctc aacaggtggc gcgacaaatt
     1501 tacaacaagt acaatttgga acattcgaat atacagagtc tgctgttaca caagtgagat
     1561 acgttgatgt aacaacaggt aaagatatta ttccaccaaa aacatattca ggaaatgttg
     1621 atcaagtcgt gacaatcgat aatcagcaat ctgcattgac tgctaaagga tataactaca
     1681 cgtccgtcga tagttcatat gcgtcaactt ataatgatac aaataaaact gtaaaaatga
     1741 cgaatgctgg acaatcagtg acatattatt ttactgatgt aaaagcacca actgtaactg
     1801 taggcaatca aaccatagaa gtgggtaaaa caatgaatcc tattgtattg actacaacgg
     1861 ataatggtac tgggactgtg acaaatacag ttacaggatt accaagcgga ttaagttacg
     1921 atagtgcaac gaattcaatc attgggacac caacaaaaat tggtcaatca acagtgacag
     1981 ttgtgtctac tgaccaagca aataacaaat cgacgacaac ttttacaata aatgttgtgg
     2041 atacgacagc accaacagtg acaccaatag gagatcaatc atcagaagtg tattcaccaa
     2101 tatccccgat taaaattgct acgcaagata acagtggaaa tgcggtgacg aatacagtga
     2161 ctggattgcc atccggacta acatttgata gtacaaataa tactattagt ggtacaccaa
     2221 caaacattgg tacaagtact atatcaatcg tttctacaga tgcgagcggt aacaaaacga
     2281 cgacaacttt taaatatgaa gtaacaagaa atagcatgag tgattccgta tcaacatcag
     2341 gaagtacaca acaatctcaa agtgtgtcaa caagtaaagc tgactcacaa agtgcatcaa
     2401 cgagtacatc aggatcgatt gtggtatcta catcagctag tacctcgaaa tcgacaagtg
     2461 taagcctatc tgattctgtg agtgcatcta agtcattaag cacatctgaa agtaatagtg
     2521 tatcaagctc aacaagcaca agtttagtga attcacaaag tgtatcatca agcatgtcgg
     2581 attcagctag taaatcaaca tcattaagcg attctatttc aaactctagc agtactgaaa
     2641 aatccgaaag tctatcaaca agtacatctg attcattgcg tacatcaaca tcactcagtg
     2701 actcattaag tatgagtaca tcaggaagct tgtctaagtc acaaagctta tcaacgagta
     2761 tatcagggtc gtctagtaca tcagcatcat taagtgacag tacatcgaat gcaattagta
     2821 catcaacatc attgagcgag tcagctagca cctcggactc tatcagtatt tcaaatagca
     2881 tagccaactc tcaaagtgcg tcaacaagca aatcagattc acaaagtaca tcaatatcat
     2941 taagtacaag tgattcaaaa tcgatgagta catcagaatc attgagcgat tcgacgagca
     3001 caagtggttc tgtttctgga tcactaagca tagcagcatc acaaagtgtc tcaacaagta
     3061 catcagactc gatgagtact tcagagatag taagtgactc tatcagtaca agtgggtcat
     3121 tatctgcatc agacagtaaa tcaatgtccg taagtagttc aatgagcacg tctcagtcag
     3181 gtagtacatc agaatcatta agtgattcac aaagtacatc tgattctgat agtaagtcat
     3241 tatcacaaag tactagtcaa tcaggttcaa caagtacatc aacgtcgaca agtgcttcag
     3301 tacgtacttc ggaatcacaa agtacgtctg gttcaatgag tgcaagtcaa tccgattcaa
     3361 tgagcatatc aacgtcgttt agtgattcaa cgagtgatag caaatcagca tcaactgcat
     3421 caagtgaatc aatatcacaa agtgcttcta cgagcacatc tggttcggta agtacttcga
     3481 catcgttaag tacaagtaat tcagaacgta catcaacatc tatgagtgat tccacaagct
     3541 taagtacatc agagtctgat tcaataagtg aatcaacgtc aacgagcgac tctataagtg
     3601 aagcaatatc tgcttcagag agcacgttta tatcattaag tgaatcaaat agtactagcg
     3661 attcagaatc acaaagtgca tctgcctttt taagtgaatc attaagtgaa agtacgtctg
     3721 aatcaacatc agagtcagtg agtagttcga caagtgagag tacgtcatta tcagacagta
     3781 catcagaatc tggtagcaca tcaacatcat taagtaattc aacaagtggt agtacgtcca
     3841 tttcaacatc gacaagtatc agtgaatcaa cgtcaacgtt taagagcgag agtgtttcaa
     3901 catcactgag tatgtcaacg agtacaagtt tgtcaacatc attaagtgat tccacaagtg
     3961 atagtaagtc tgattcatta agtacatcaa tgtcgacaag tgattcaatc agtacaagta
     4021 aatctgattc cattagtaca tccacatcat taagtggttc tacaagtgaa agtgaatccg
     4081 actcaacatc atcaagtgaa agtaaatccg attcaacatc aatgagcata agtatgtctc
     4141 aatcaacatc aggaagtaca agtacgtcaa cgagtacaag tttgtctgac tcaacgagta
     4201 cgtcattgtc actaagtgca tcaatgaatc aaagcggagt agactcaaac tcagcaagcc
     4261 aaagtgcctc aaactcaaca agtacaagca cgagcgaatc cgattcacaa agcacatcat
     4321 catatacaag tcagtcaaca agccaaagtg aatccacatc gacatcaacg tcactaagcg
     4381 attcaacaag tatatctaaa agtacgagtc aatcaggttc aacaagcaca tcagcatcat
     4441 taagtggttc agagagtgaa tctgattcac aaagtatctc aacaagtgca agtgagtcaa
     4501 catcagaaag tacatcaaca tcgcttagtg actcaacaag tacaagtaac tcaggatcag
     4561 caagtacgtc aacatcgctc agtaactcag caagcgcaag tgaatccgat tcgtcgtcaa
     4621 catctttaag tgattcaaca tctgcgtcaa tgcaaagcag tgaatccgat tcacaaagca
     4681 catcagcatc attaagtgat tcgctaagta catcaacttc aaaccgcatg tcgaccattg
     4741 caagtttatc aacatcggta agtacatcag aatctggctc aacatcagaa agtacaagtg
     4801 aatccgattc aacatcaaca tcattaagcg attcacaaag cacatcaaga agtacaagtg
     4861 catctggatc agcaagtaca tcaacatcaa caagtgactc tcgtagtaca tcagcttcaa
     4921 ctagtacttc gatgcgtaca agtacaagtg attcacaaag tatgtcgctt tcaacaagta
     4981 catcaacaag tatgagtgat tcaacgtcat tatctgatag tgttagtgat tcaacatcag
     5041 actcaacaag tgcgagtaca tctggttcga tgagtgtgtc tatatcgtta agtgattcga
     5101 caagcacatc aacatcggct agtgaagtaa tgagcgcaag catatctgat tcacaaagta
     5161 tgtcagaatc tgtaaatgat tcagaaagtg taagcgaatc taattctgaa agtgactcaa
     5221 aatcgatgag tggttcaaca agcgtaagtg attctggctc attgagcgtc tcaacgtcat
     5281 taagaaaatc agaaagtgta agcgagtcta gttcattgag tggctcacaa tcgatgagcg
     5341 attcagtaag cacaagcgat tcgtcatcat taagtgtatc gacgtcacta agaagttcgg
     5401 aaagcgtgag tgaatctgat tcattaagtg attcaaaatc gacaagtggt tcgacttcaa
     5461 caagtacatc tggttcattg agtacctcaa catcattaag tggttcagaa agcgtaagcg
     5521 agtctacctc gctaagtgat tcaatatcaa tgagtgattc tactagtaca agtgactccg
     5581 actcattaag tggatcaata tctttaagtg gttccacaag tcttagcact tcggattcat
     5641 taagtgattc aaaatcattg agtagctcgc aaagtatgag tggatcagaa tcaacgtcaa
     5701 caagtgtgag cgattcgcag tcaagctcaa caagtaatag tcaatttgac tctatgagca
     5761 tcagtgcatc agaaagcgac tcaatgtcta caagtgattc gtctagcatc agtggatcaa
     5821 attcaacgag tacatcactt tcaacatctg actcaatgag cggaagcgta tcagtttcaa
     5881 catcgacaag tttaagtgac tcaatatcag gttcaacaag tgtaagtgac tcaagctcaa
     5941 cgagtacatc agagtcgtta agtgattcta tggcacaaag ccagtcaaca agtacaagtg
     6001 catctggttc cttaagtaca tcgatatcaa catcaatgtc aatgagtgca agtacatcga
     6061 catcacaaag cacgtcggtg tcgacatcat tatcaacatc agacagtatc agtgattcta
     6121 cttcaataag tatcagtggt tcacaaagta cagtagaatc agaatctaca agtgattcaa
     6181 cttctatcag tgactcagaa tcattgagta catcagattc agactcgaca tcgacaagta
     6241 catcggactc aacaagtagt tcaacttcaa caagcatatc tgaatcatta agtacgtctg
     6301 gttcaggttc aacgagcgta tctgactcaa catcaatgag tgaatccgat tcaacaagtg
     6361 tttcaatgtc acaaagtatg tccggttcaa cgcacaactc gacttcagtg agcgactcag
     6421 aatcagtgtc aacaagcaca tcaacgtcat tgagcacatc cgattcgaca agcacatccg
     6481 aatcactgag tacatctatg tctggttcac aaagcatttc tgactcaaca tcaacaagta
     6541 tgtccggctc aacaagtaca tctgaatcta actcaatgca tccgtcagac tcaatgagta
     6601 tgcatcatac tcacagcacg agcacatctc gcttatcaag tgaagcaaca acgagcacga
     6661 gtgaatctca gtctacatta agtgcaacat ctgaagtgac taaacataat ggcacgccag
     6721 cacaaagtga aaaaagattg ccagatacag gtgactcaat aaaacaaaat ggattactag
     6781 gtggcgttat gacattatta gttggtttag gtttaatgaa gagaaagaaa aagaaagatg
     6841 aaaatgatca agatgattct caagcataaa ttgaatttat agctggctaa aattaaaaag
     6901 actgaggcac caatcactag aaaaatcgat ataggatttt aataattagt gatgaaccct
     6961 tagtcttttt aatgtttttg atataaaaga ggtgcaacat gttaaaactt ttacaacaat
     7021 acgaatataa aattatatat aaacgtatgc tatacacatg ttttatcttg tttatttata
     7081 tattaggcac aaacatttcc atcgtttcct ataacgatat gcaagtaaaa catgagtcat
     7141 tttttaaaat agctatttct aatatgggtg gagacgttaa tactttaaac attttcactt
     7201 taggattagg tccgtggtta acatcaatga ttatcttgat gctgatttct tatcggaata
     7261 tggataaata catgaaacaa acaagtcttg agaagcatta taaagagcgc atcttaacat
     7321 taattttaag tgttattcaa agttattttg tgattcatga atatgtttcg aaagaacgtg
     7381 ttcatcaaga caatatttac ttaacaatat tgattttggt tactggaacg atgttattag
     7441 tttggttagc tgacaaaaat agtcgctacg gcatcgcagg accaatgccg attgtaatgg
     7501 ttagtattat taaatcaatg atgcatcaaa aaatggaata tatagatgca agtcatattg
     7561 tgattgcgtt gttaatcatc cttgtgataa tcacattgtt tatcttatta tttattgaat
     7621 tagtagaagt gagaatacca tatatcgatt taatgaacgt ttccgcaaca aatatgaaat
     7681 cttatttatc ttggaaagtt aaccctgcag gcagtattac tttaatgatg agtatttcag
     7741 cttttgtttt cttgaaaagt ggcattcatt ttattttatc tatgtttaat aaaagcatat
     7801 cagatgacat gccaatgctg acatttgata gtccagtagg tatttcagta tatttagtga
     7861 ttcaaatgtt attgggttat tttttatcga gatttttaat caatactaaa caaaaatcca
     7921 aagatttctt aaagagtggc aattattttt caggagttaa acctggtaag gatacagaac
     7981 gttatttaaa ttatcaagca cgacgcgtat gttggtttgg atcggcatta gttacagtca
     8041 ttattggtat accgctttat ttcacattgt ttgtaccgca tttatctact gaaatttatt
     8101 tctcagtaca actgattgta ttagtttaca tcagtattaa tattgcagaa acaattcgta
     8161 catatttata ttttgataaa tataagccat ttttaaacca gtattggtaa ggaggtaatt
     8221 atgaaatact ttattccagc ttggtacgat gaccaacgat ggtggcaaga cacgactgtg
     8281 ccgtattatc aactacaaaa taagacggaa tttgacgata tgattagttt aatgggaatg
     8341 caccttgaaa atgacttaga ttatcaactg attgttctca atcatgcacc aaatttaaga
     8401 acatttttac atcgatatga cttatatgag acaaagtatt cgtctgtgtt tgatgaaatt
     8461 caaggattca gtcaccatgc gccacaagcg attaattatc atcacttaaa atggccggat
     8521 gatgttgagt ttgtgtacac accgtattta ttaaaatgtg tgacgagtga acagacctat
     8581 acaaatattt attttagtca agaagggtat tcaatttggt ttgaagaatt tgaaagagat
     8641 cagttacaac ggcgttatat ttttgatgac agaggttatt tatcagcgat acgttatttt
     8701 gatgatcagg gagaggcttc ttaccaagaa tatttaacga ttaatggaga ttgtgtactt
     8761 catgaagatt tgaaaaatgg cagagtcact gtatcaaaaa gatatcaaca tcactatcaa
     8821 caaacagaat ataacaatat ggctcaacta attgaagaaa aatttcaagc aatgattgca
     8881 caacaaatac atgaagatga tcatgtgatt gtggcttcag atgctaggca caatcgacaa
     8941 atagccaatc atattccagc gaaattgtta agttattcat tttttaaaaa tagaaatgaa
     9001 actgtgtcag atgaggaata tcaatctatc gtaaagaatg cccatttaat tgttgatagt
     9061 gtgcaactag aacgtgattt aattagtcat caagagaagt atcagcggga gaatacaatg
     9121 attcgaatca caccatttga aacgagacaa tcacctaata taagtagtca attgatggaa
     9181 acatttatag gtgtatggat agatggtatg agtgacgctg atttgcaaca aatgatgcaa
     9241 cgacttgtgg attatatcgc acaggaagat tattaccgtt taattttatt atcgcgccat
     9301 caaaatgaca taccgatgtg gcttcgtgaa tgtattacgt cggtaaatga ggaataccaa
     9361 gctaaacaga atgcggatgt taatgtttca gcattaatga cacctgaaga tcaagatgac
     9421 atcattgctg ttaagacgat acatgctgaa catgatgttg tagaagcatt gcggacgttg
     9481 cgacttgtga tagatatgtc aaaagaacct gacttgtatt tacaaattag tgcaattagc
     9541 gctgggattc cacaaattaa tggtcaacaa acagattacg tctctgatta tgacaatggc
     9601 cgtattataa atacagttga tgaattagat gatgcgttaa attattattt attttatttg
     9661 aaaaattgga attatgcgta cgcctattct ttaaaattaa tagatgcata tgcttctaag
     9721 aatattatta atcagctcga tgagttaata gaaggtgaaa atgatgccac gtaaatttag
     9781 agttttgcaa attggaggag acgatttaga acctattttt caacacaaaa aaggtgtgag
     9841 ttgggattac ttcgatattg gattgtttga atttgatagt ggttatgtag aggctattga
     9901 agcgattgtt gaagcagaag ggcgctttga ttttatctat attcaagcac catactcgga
     9961 gacattaacg aatttattac aaatgataag cgaaccatac aatacgtatg ttgatgaatc
    10021 attttggtca gttgaatatg aacaagacga aaatgtccaa aaatacgttg ttcaaccatt
    10081 acattaccgg aatattgaag aacgtaataa taaattagag gcagttagct tctcaggaca
    10141 gtatggagat aaggtttctc ctaagttagc acttgtgcat ccgaatttta aaggagatgt
    10201 cgtttaccaa ggtaattcag agctcacatt gagtggagaa tttgggaaag aatttaaacc
    10261 tatcgcatct tggcaaaata atctcgttta cgataaagat aaagtcattc aaatatggcc
    10321 agaatttgat attgatggtg cgttcgagtt gcaatataca tttagattga ttcagactgg
    10381 cgctgatggt gcattaattg aacaaatcgt attgactgat gatatgttag acagtccctt
    10441 agagatacct gcgaaaccat ttgatgctta tataagtgta actgttaagg cgcgtgggaa
    10501 cgggacggta catttaggac ctatacacaa acgatggtct agattagaca tgggtcaatt
    10561 tttacttggt ggtagtcgtt tcgtagatag tcaacgacag gaatttattt attatttcca
    10621 ccctggtgat atgaaaccgc cactaaacgt atattttagt ggttatcgaa cagcggaagg
    10681 tttcgaagga tattatatga tgaaacgtat gaatgcaccg tttttactta tcggcgatcc
    10741 tcgtgttgaa ggtggtagtt tttatatcgg ttcatctgaa tatgaacaag gtattatcaa
    10801 tgttattgac gagacattag aaaagctgaa ttttaaatca catgaattaa tattatctgg
    10861 tttatctatg ggttcatttg gtgccttgta ttatggtgct cagttaaatc ctcaggcaat
    10921 tattgttggt aaaccacttg ttaatattgg aacgattgct gaacatatgc gattacttag
    10981 accagaagag ttcggtacag cattagacgt cctggtgtct aatgaaggtg atacatctca
    11041 agcgtcgata caggcattaa atcaaaaatt ttggcaaaca tttcaaaaga aatcattgtc
    11101 acaaactgtt tttgccatag catatatgca acatgatgat tatgatccac atgcatttca
    11161 agagctatta cctgtattga cagcacacca agcaagagtg atgaatcgca gtattcctgg
    11221 gcgacataat gatgattcac ctaccattgc aagttggttt gttaattttt acaacattat
    11281 tttggaagac aagtttggga gagttcaaca tgctgaaaaa caaaacattt aaagttactt
    11341 ggaattatat ttcacaaact acatttatgt atggctctaa agtatcattt tcaaatggtg
    11401 aagtgacttt tataaatcct ttgatgccct ctggattacc gattcatgag tggttgatgt
    11461 taaagcaatt ttcaaaatat aaaagtgcgc caagtttacc aattttgcgt agaggacagc
    11521 attataaatt gcattttgat tttgatgcaa cgcctgctgg ttcagtgtat tttatcatca
    11581 ttttttataa taaaaatggc acaaaactaa gtactgagat tgtaaagtca aatagtatta
    11641 cgattcaata tcctgatgaa gcctatgctt ataaaataaa aatgatgaat gcagcatcga
    11701 cgagtcttat atttagatgt ttaacaatca cagagatgac gcatcaagat gatttagagt
    11761 ataaaagcat gcgggtgacg aagataggcg atgaccaata tggaaatgat atgatcaatg
    11821 tcattattgc agaaccatct gacacctatc caactatttc gaatgatttt ttgaaactgt
    11881 ttggtcatgt ttggctcgtt gaacgttgga tggacgacaa tatcgaggga aatattaagc
    11941 aactaaagaa tgatttgcaa agtcaagata ccctaaaggc tattaatttg attagttatg
    12001 ggtcaaagtc aaatgttttt gcaacttatg tcgctcagca tttgggttgc aaagtgtatc
    12061 gtacaagcca tgaagatgat gatttaaaag gatggcttac agaacatgtc ccaggaaatc
    12121 atgaactaaa agatacaaat gtcgaggttt attttaaaga agagcaagac aaacatctaa
    12181 attatatgtc acgtttaatg aatccagtac gatttttaga ttacttagat gtatcgaaac
    12241 tgaatggtgg ggaagtaaat gaaacataaa ctagatgtta cgataaacga attaagacta
    12301 aagtcgatta gaaaaatagt gaaacgcatc aatacatgga gcgatgaagt taaatcatat
    12361 tctgatgatg cgttaaaaca aaagacaata gaatttaaag aacgtttagc atcaggagtt
    12421 gatacactag atacattgtt acctgaagct tatgcagtgg cacgtgaagc gagctggaga
    12481 gtattaggta tgtatcctaa agaagttcag ttaatcggag cgattgtatt gcatgaaggt
    12541 aacattgcgg agatgcaaac aggcgaaggt aaaacattaa cggcaacgat gcctttatat
    12601 ttaaatgctc tttcgggaaa aggaacatat ttaattacaa cgaatgatta tttagcgaag
    12661 cgagattttg aagagatgca accattatat gaatggttag gtttaactgc ttcattaggt
    12721 tttgttgata ttgtggatta tgagtatcaa aaaggggaaa aacgtaatat atatgaacat
    12781 gatattatat atacaactaa tggacgtctc ggttttgatt atttaattga taatttggct
    12841 gatagtgcgg aagggaaatt tttaccacaa ttaaattacg gtatcattga tgaagtggat
    12901 tcaatcattt tagatgctgc tcaaacacca ttagttattt cgggtgcacc aagattacaa
    12961 tcaaatctat ttcatattgt gaaagagttt gtagatacat tgattgaaga cgtgcatttt
    13021 aaaatgaaga agaccaaaaa agaaatatgg ctgttaaatc aaggtattga agcggcacaa
    13081 tcatacttta atgttgaaga tttatatagc gaacaagcga tggtcctagt gcgtaatatt
    13141 aatttagcac tgcgcgcaca atatttgttt gaatctaatg tcgattactt tgtatataat
    13201 ggtgatattg ttttaattga ccgtattaca ggtcgtatgt taccgggaac taagttgcaa
    13261 gctggacttc accaagctat tgaagcgaaa gaaggtatgg aggtttcaac agataaaagt
    13321 gttatggcaa caattacctt ccagaattta tttaaacttt ttgaatcatt ttcaggtatg
    13381 acagctacag gaaaattagg cgaatcagag ttctttgatt tgtattcaaa aatagtcgta
    13441 caagtaccaa ctgataaagc gattcaacgt atcgatgaac cagataaagt gtttcgttca
    13501 gttgatgaga aaaacatcgc gatgattcat gatatagttg aacttcatga aacggggcga
    13561 ccggttttac tcataacgag aactgctgaa gcggctgaat acttttcgaa agtattattc
    13621 caaatggata ttcctaataa tttactcatt gcgcaaaatg ttgcaaaaga agcgcagatg
    13681 atagctgaag cagggcaaat tggttccatg actgttgcga ctagtatggc aggtcgaggc
    13741 acagatatta aacttggtga aggtgtcgaa gcattaggtg gattagctgt tattattcat
    13801 gaacatatgg aaaatagccg tgtagacagg caattacgtg gtcgttctgg tagacaaggg
    13861 gatccgggat catcttgtat atatatttca ctagatgatt atttagttaa gcgatggagc
    13921 gatagtaatt tagcggaaaa taatcaatta tattcattag atgcacaacg attatcgcaa
    13981 agtaatttgt ttaatcgcaa agttaagcaa attgtagtta aagcgcagcg tatctcggaa
    14041 gaacaagggg ttaaagctcg tgaaatggct aatgaatttg aaaaaagcat tagtatacag
    14101 cgagatcttg tatacgagga acgcaatcga gttttagaaa tagatgatgc tgagaatcga
    14161 gattttaaag cgttagctaa agatgtattt gaaatgtttg taaatgagga aaaggtgcta
    14221 acaaaatcgc gtgttgtcga gtatatttat caaaatttaa gtttccaatt taataaagat
    14281 gtggcttgtg ttaattttaa agataagcaa gcagtagtga catttttatt agagcaattt
    14341 gaaaagcaat tagctttgaa tcgtaaaaac atgcaaagtg catattatta taatattttc
    14401 gttcaaaaag tctttttgaa agcaattgat tcatgttggt tagaacaagt cgactattta
    14461 caacaattaa aagcaagcgt taatcaacga caaaatgggc aacgtaacgc tatttttgaa
    14521 taccatagag ttgcattaga ttcatttgaa gttatgacac gaaatatcaa aaaaagaatg
    14581 gtgaaaaata tttgtcaaag catgattaca tttgataaag agggtatgcc agttatacat
    14641 tttccataaa tgaaaagggg tagcgcatga caatatataa tataaacttt ggtataggct
    14701 gggcgagtag cggtgttgaa tatgcacaag cttaccgtgc taaattattg agacgtacaa
    14761 atcaagacac taaatttgtg tttttagatt ttatacagtc agagaacata cagacattaa
    14821 cgcaaaacat tgggtttaaa gatcaagaag ttatttggtt gtatcagtat tttagtgata
    14881 tttctatagc accaacaacg tacacgttag atgacattga acgtgagctt ggtcatgaaa
    14941 ttagttttag agaaagaaat ggtaagatag tccgtctata ttttaatggg aaatcatcat
    15001 ttgtaacatg ttatctacaa aatgaacaaa aagatgttgt ggatcgtgca gaatttgtaa
    15061 ttaatagtat gttagtacgt aaagattttt atagttatac gcgtatattt tctgagtatt
    15121 atgcaccagc tgataataaa gcgaaattat atatgcgaca attctataat gaagatggtt
    15181 ccatcgctta taacgaatat attgatggag acagctctat atatgtgttt gaagatgcgg
    15241 tgttgtataa caaaacggaa tttattgcat actttttaca acgattgaat ttaacccgag
    15301 acgatattgt catattagac cgcgcttctg atattggtca ggctgttttg cagcataaag
    15361 gtgatagtaa agttggtgtt gtgattcatg ctgatcatta tagtaataat atgatgagtg
    15421 agcagcatat actatggaac aattattatg aatatcaatt ttcaaaagct aaatacattg
    15481 atttctttat tacagcaaca gatattcaaa atcatatggt ttgtcgacaa tttgagcaat
    15541 atcaaggtta tcgcccacgt gtttatacta ttcctgtggg aagcattgat gcattatcat
    15601 atccaacatt atcaagaaag ccatatgcga tgattagtgc atctcgttta gcgaatgaga
    15661 aacatattga ttggcttgtc aaagcggtta tcgtggctaa acgacaagtg ccagaattaa
    15721 catttgatat ttatggagaa ggtagcgaga agacgcgact gcgtaaaatt attgatacac
    15781 accgcgcaca agattatatt cgtttactag ggcatgttaa acttgatgaa atttataatg
    15841 attatgagtt atttctttct gcatcaacta gcgaaggttt tgggttaaca ctaatggaag
    15901 cggtaggctc aggactaggt atgattggat taaatgtgaa ttatggtaac ccaaccttta
    15961 ttcgagatgg ggaaaatggc tacttggtac cttttgatac cgacgaagat agtgtggatg
    16021 atgttatcgc aaaactagct catgccattg taatgtattt taacaatggt ccccagacac
    16081 cacatgacat ctcttatgaa gtggcacagc aatttatgac tcaagatatt attttaaaat
    16141 gggaaacatt agttcaagag gtgctacatg attaaattat ttgattactt taacgatcat
    16201 tcacgaaagt tatatgagtc ttttaaagcg agtaagttgg aagaagattt aacaattgta
    16261 ctcaatgaca atggcttttt accggacgat gttatatcac cgtatcaatt ttttgcagat
    16321 aatcataata cagaaaatat gaaaccacga ttttttaatc aggttactgt accagctttt
    16381 tgggaaatta aaggtaacaa caattcggct acaattaatg atatgggacg tctacgaggc
    16441 aaaatatttt atcaaagtgg agagcgccct agaattgtta gtcgtgtcga atggtttgat
    16501 gaccaacaac gtgtccgatt tgtcgattat tattctaaga atggtattaa atttgcacaa
    16561 actgtttacg atttaaatcg taaagcgatc ttgaagaaat atatgacagc agaaggtaaa
    16621 gaagtgattt atgaaaattt tgtaacatct gatgtcatat tggattggca agggaagtct
    16681 tactttttcc cttcaaagct cgcattcgta ttatttttta tcaagcaact tgaaattacg
    16741 gaacatcatt tcgttattaa ctcactagcc ctaccatttt cagtgttata taatttgcca
    16801 tcaaaaggta gtgatgtttt ggtatggcaa gaacactgta atggtaatgt cccgggaaat
    16861 atgcaattga tgtgtaaagg tgatatgaaa cgacattgta atattattat tccagataaa
    16921 aacgaatatg aaacgatgct caatatagca gacgcaaagg ttcaatctcg tattttacaa
    16981 ggcggatatc tatataacta tcgtagtcgt aatagatata ctaaagaaat tgtaatatta
    17041 actaactcgg atcaacttcg taacatcaag gtattggttg aaactttacc agattttaag
    17101 tttcacatcg ctgctattac agagatgtct gataagctaa tgcaacttga tcaatatgct
    17161 aatgtgcatt tatacccatc tattaatatt gacagggtaa atgaactata ccagttatgt
    17221 gatatttatc ttgatatcaa tgagggtaat gaaattttaa atgcggtgga acaagcgttt
    17281 gactatgagt tgttgatttt aggttatcgt caaaccgcac accatgctaa agtaacatta
    17341 tcagagcact tatttgagca taatgatgag ataacaatgg aaagtaaaga ccaattaata
    17401 caaatgttag agagtttgaa agatcaacaa cagtttcgag atgctttatt agcacaaaaa
    17461 gcacatgcac acgaaatctc gcgtgaacaa ttcgagcaag tttttaaaca ggcgctcgaa
    17521 agctaaaaag aaaaagcagt ctaaaataaa acatcattta atataaaaga tttgacgtaa
    17581 tatgatgtta gcgacatgga caatatgata ttacgtcatg cttttttgtt tacttaaata
    17641 taagctaagt aataagtgga taattactaa caacaataac tagatagata agtgtaaatt
    17701 tcttgtaaac aggtatataa tagtatgtaa ttctttgaag gaagtaagtg agaaaggagt
    17761 atgttgatgg ctaaatatcg agggaaatcg tttcaattat atgtaaagtt atcgtgttcg
    17821 acaatgatgg cgacaagtat cattttaacg aatatcttgc cgtacgatgc ccaagctgca
    17881 tctgaaaaga atactgaaat ttcaaaagag atattatcta agcaagattt attagacaaa
    17941 gttgacaaag caattcgtca aattgagcaa ttaaaacagt tatcggcttc atctaaagca
    18001 cattataaag cacaactaaa tgaagcgaaa acagcatcgc aaatagatga aatcataaaa
    18061 cgagctaatg agttggatag caaagacaat aaaagttctc acactgaaat gaacggtcaa
    18121 agtgatatag acagtaaatt agatcaattg cttaaagatt taaatgaggt ttcttcaaat
    18181 gttgataggg gtcaacaaag tggcgaggac gatcttaatg caatgaaaaa tgatatgtca
    18241 caaacggcta caacaaaaca tggagaaaaa gatgataaaa atgatgaagc aatggtaaat
    18301 aaggcgttag aagacctaga ccatttgaat cagcaaatac acaaatcgaa agatgcattg
    18361 aaagatgcat cgaaagatcc agcagtgtct acaacagata gtaatcatga agtagctaaa
    18421 acgccaaata atgatggttc tggacatgtt gtgttaaata aattcctttc aaatgaagag
    18481 aatcaaagcc atagtaatcg actcactgat aaattacaag gaagcgataa aattaatcat
    18541 gctatgattg aaaaattggc taaaagtaat gcctcaacgc aacattacac atatcataaa
    18601 ctaaatacgt tacaatcttt agatcaacgt attgcaaata cgcaacttcc taaaaatcaa
    18661 aaatcagact taatgagcga agtaaataag acgaaagagc gtataaaaag tcaacgaaat
    18721 attattttgg aagaacttgc acgtactgat gataaaaagt atgctacaca aagcatttta
    18781 gaaagtatat ttaataaaga cgaggcagat aaaattctaa aagatatacg tgttgatggt
    18841 aaaacggatc aacaaattgc agatcaaatt actcgtcata ttgatcaact atctctgaca
    18901 acgagtgatg atttattaac gtcattgatt gatcaatcac aagataagtc gctattgatt
    18961 tctcaaattt tacaaacgaa attaggaaaa gctgaagcag ataaattggc taaagattgg
    19021 acgaataaag gattatcaaa tcgccaaatc gttgaccaat tgaagaaaca ttttgcatca
    19081 actggcgaca cgtcttcaga tgatatatta aaagaaattt tgaataatgc caaagataaa
    19141 aaacaagcaa ttgaaacgat tttagcaaca cgtatagaaa gacaaaaggc aaaattactg
    19201 gcagatttaa ttactaaaat agaaacagat caaaataaaa tttttaattt agttaaatcg
    19261 gcattgaatg gtaaagcgga tgatttattg aatttacaaa agagactcaa tcaaacgaaa
    19321 aaagatatag actatatttt atcaccaata gtaaatcgtc caagtttact agatcgattg
    19381 aataaaaatg ggaaaacaac ggatttaaat aagttagcaa atttaatgaa tcaaggatca
    19441 aatttattag acagtattcc agatataccc acaccaaagc cagaaaagac gttaacactt
    19501 ggtaaaggta atggattgtt aagtggatta ttaaatgctg atggtaatgt atctttgcct
    19561 aaagcggggg aaacgataaa agaacattgg ttgccgatat ctgtaattgt tggtgcaatg
    19621 ggtgtactaa tgatttggtt atcacgacgc aataagttga aaaataaagc ataattatat
    19681 tgggggaaga gcatctatat atttttttaa gtatataaga cgtcttattt ccccttaaat
    19741 tattgtgaag tatatgcaaa atgcaatgaa tagattgtcc atcattttaa cgttataatg
    19801 aatttaacga cttagaacta cagaagtaaa ggagaatgaa gatgtctcga aaaacggcgc
    19861 tattagtttt ggatatgcaa gaaggtatag cgagtagtgt acctagaata aaaaatatta
    19921 ttaaagcgaa tcagagagca attgaagcag caagacaaca tcgaatacca gtcattttca
    19981 tacgtttagt gttagataag cattttaatg atgtctcctc gagtaataaa gtgttttcaa
    20041 caattaaagc tcaaggatat gcgattactg aagcagatgc atctacacga atacttgaag
    20101 acttagcacc actagaagat gagccgatta tttctaagcg acgctttagc gcatttacag
    20161 gtagttactt ggaagtttat ttacgtgcaa atgatattaa tcatttagta ttaacgggtg
    20221 tctctacaag tggagctgta ttgagcacgg cattagaaag tgtagataaa gactattata
    20281 ttactgtttt agaagatgct gttggtgata gatcagatga taaacatgac tttattattg
    20341 aacaaatttt atcacgctca tgtgacattg aatccgtaga gtcatggaaa agtagtttat
    20401 agttaatata acgtcaatta aagctcggca gtaatgttta agaataagta catttgctca
    20461 tatttataaa atgtgtgaga tggcaattga aacggatatg atgaggaaca tttgaacata
    20521 aaataatata tttatataaa atgacccgag gcgttcgaac tgaatgcctc gggtttaatt
    20581 gaataaggaa tcggacttat gaacagaaat atgtttaagt ccgaactcct tgtttatact
    20641 tataaatttt acgggtttaa tataatactt atttacctgt aatatatgat aattcttcag
    20701 cggcagctgc gttgatagtt ctatgagaaa tgatacctaa tcctttaaca ttggattctg
    20761 aaataacgat agaaccatca ctgttaactt tttcaacaaa tgctacatga ccgtaatgtt
    20821 gatctgcacc aaattgtcca gcctcaaata caacagcagc atgacgtttt ggtgtatgac
    20881 ttacttgata atcacggtat tgagctcgat tattccaatt atgtgcatca cctaaatcac
    20941 ctgagataga tgtaccaaat tgtttcatac ggttatatac gtaccaagta cattggccat
    21001 gtggatatgg catactatca gatacctcac ggaaaggttt gaattcatct gatgaatcat
    21061 cataatcctt gatagaacgt tcatatttat ctaaatctgg catgcgttca tcgtcaaact
    21121 gagttaattg atagtgttta ataatactgt ttaatttctt agcatagttt ggatctgtag
    21181 catatgtttt agataagtgt gatgttgcat ctttataaga atcggcttcc gatttccatg
    21241 ttggtttata aattgttcga ttgccatcaa taccattttt aataaggtca gagtaatctt
    21301 ttagtgattc tttcgtgctt ggatattttc ggaatccagc attaatacta tacaattgat
    21361 taccatcagc ttctaatgtg ttaaaaggaa cagaattccc ttcaaaagca cctttgatac
    21421 cgaataaatt atggtttggt gacttagcta aagcactacg acctgagtca gattctaaga
    21481 ttgcttgggc aatcatgaca gacgcataaa tatcgttatc ttgaccaatg cgatgtgcat
    21541 ctttagcaat tgatttgaca aattgacgtg tatcttttga gtcaacaacg ttaaattgtc
    21601 cactatcatc attgttagat atactaggat ctgtttcgaa taatgatgtt gcacgtgtat
    21661 ccttttgatt aatatcgtta ttgaatgatt gagcaggttt agatttatgt ttcaattcat
    21721 cttgtgttgg taactgtgga ttctttgtat tagatttttc atttttgtct tttttagatt
    21781 gagatgcata atctttttgt gttttctttg catcttcact gtattgatcc aaaatagagt
    21841 ctaaagccga atctgacatt gattgattat ctttcgatga agatttttga tttgctttat
    21901 cgtcacttgc tggttgacta tttgattgat taggttgtgt tggctttggc gaatttggtt
    21961 gcttattaga tgtacttggt tttgtattgt ttgatttagg tgctttttga ttgtctgctt
    22021 tatcttgttt agatgattgc gtatcagtgt catttttgat gctattgtca ctgtttttat
    22081 tcgaatcatt tgttgacttt tcgtcattac gaggttgttc gtaatcagaa atatccgaat
    22141 ttaaattgaa taagttttgg attaaagttg ttaatgagta attatcatcg tatttatttt
    22201 tggttagcaa ttggtttata ttggtttgtg gtaaattctt ataaataaaa tcaatgatat
    22261 tgttagagtc tgaagtgctg tcgtctatag ttttaaattt tttgtcgtta ttgtcttggt
    22321 tacttgtatt atttttgtct gctttatcaa tatctttact tgtagtatcc ttagaagttt
    22381 catcgtcatt agattttttt gaatcatgag atgttgtctt agctgtagta tctttttgag
    22441 gtgtatcagc ataagcggta ggtgaaacta aagtaggtaa tacgagcgta gttgatagca
    22501 aataaattaa aattttattt ttaggcatat ttcgtattct cccttgaaaa atataataat
    22561 taagtgtgat aataaactat gatttgttat aatttatcgt atgctgaaaa tagttgatag
    22621 gtatcaatcg actaaatatc ttccagtaaa ttgattatac taattcacaa cgcaaaaata
    22681 aattaattta caaaaaatat ataaaaaata tgaataattc ctacatagga gtgtgacaat
    22741 gaagaacgca tttaaattat ttaaaatgga tctgaagaaa gtagctaaga cgccagctgt
    22801 gtggattatc ttagcaggct tagctatttt gccatcgttc tacgcttggt ttaacttatg
    22861 ggcaatgtgg gatccatatg gcaacacggg acacatcaag gtcgcagtcg ttaatgaaga
    22921 taaaggcgac acaatcagag ggaaaaaagt taatgtcggt aatacgatgg ttaatacact
    22981 caagaaaaat aaaagttttg attggcagtt tgtaagtaga gagaaagctg atcatgagat
    23041 aaaaatgggt aaatattttg caggtattta catcccatct aagtttacac atgaaattac
    23101 agggacacta cgtaagcagc ctcaaaaagc agatgtagaa tttaaggtga atcagaagat
    23161 taacgctgtt gcgtctaagc taacagatac tggttcgtca gttgtcgttg aaaaagcgaa
    23221 tgaacaattt aataaaacag taactcgagc attattagaa gaagctaaca aagcaggttt
    23281 aactattgaa gaaaatgtgc cgacaattaa caagataaaa aatgcggtat attcagcaga
    23341 taaagcttta cctaagatta atgactttgc gaataaaatt gtatatttga ataaccacca
    23401 agcggattta gataaatatg ccaatgattt tagaaaacta ggaaattata aaggtgatat
    23461 tttagatgct cagaaaaaat taaacgaagt caatggtgct attccgcaac ttaatgaaaa
    23521 ggctaagttg atattagctt taaataatta tatgccgaaa attgaaaaag cgttaaattt
    23581 tgtagctgat gacgtgccag cgcagttccc taaaattaat caaggactta acattgcgag
    23641 tcaaggtatt gatcaagcta atggacagtt aaatgatgcc aaaggcttcg tcacacaagt
    23701 tagaagtaga gtcggtgatt atcaagatgc aattcgacgc gcgcaagatt taaatcgaag
    23761 aaaccagcaa cagattcctc aaaatagcgc ggcgaacaac gaaacatcaa atagtgcacc
    23821 tgcagctggt aatggtgtag catcaacgcc accaagtgca ccaagtggcg atactgcacc
    23881 aaataataat gttacgcaaa ataccgcacc aaatagtaat aatgcgcctg tatcgactac
    23941 accacaaagt acaagcggga aaaaagatgg tcaaagtttt gcagatataa caacaacaca
    24001 agtcagcaca gctaacgaga acacacaaaa cattacagat aaagatgtta aatcaatgga
    24061 agcggcatta acgggctctt tattatcatt atcaaataat ttagataccc aagcgaaagc
    24121 cgcacaaaaa gatagtcagg cattacgtaa tatttcgtat ggcattttag catcggacaa
    24181 gccttctgat tttagagagt ctttagataa tgttaagtcc ggtttagaat acacaacgca
    24241 atataatcaa caatttatcg atacattaaa agagattgag aagaatgaaa atgttgattt
    24301 atcaaaagaa attgataagg taaaagcagc taataatcga attaatgaat cattaaggtt
    24361 agttaatcaa ttaagcaatg cattaaagaa tggtagttca ggaactgctg aagctactaa
    24421 attactagat caactttcaa aactagattc atcattatca tcatttagag attatgttaa
    24481 aaaagatctt aacagctctt tagtatcaat atcacaacgt attatggatg aattgaacaa
    24541 agggcaaact gcattatcca atgttcagtc taaattaaat acaattgatc aagtcattaa
    24601 cagtggacaa gctattttaa aaaatggtaa aacacgtatt gatcgcttac aaacagtatt
    24661 accaagtatt gaacaacaat acattagtgc tgttaaaaat gctcaagcaa acttcccgaa
    24721 agtgaaaagt gatgtagcta aagctgctaa ctttgtgcgc aatgacttac cacagttgga
    24781 acagcgatta actaatgcga cagcaagtgt gaataaaaat ttaccaacgt tattaaatgg
    24841 ttatgatcaa gcggtaggat tactaaataa aaatcagcca caagcgaaaa aggctttatc
    24901 agatttagct gatttttctc aaaataaatt gcctgatgtt gaaaaagatt tgaaaaaagc
    24961 gaataaaatt ttcaagaaat tagacaaaga tgatgcagtc gacaaattaa tcgacacact
    25021 taagaatgat ttgaaaaagc aagcgggtat tattgcaaat cctattaata agaagactgt
    25081 tgatgttttc tcagttaagg attatggttc aggtatgaca ccattctata ctgcactgtc
    25141 agtatgggta ggtgcactct tgatggtaag tttattaacg gttgataata aacataagag
    25201 tctagagtca gtcttaacga caagacaagt gttcttaggt aaggcaggat tctttataat
    25261 gcttggtatg ttgcaagcac tcattgtatc ggttggagat ttgttaatcc taaaagcagg
    25321 agttgagtca cctgtattat ttgtacttat aacgattttc tgttcgatta ttttcaactc
    25381 aatcgtatat acgtgcgtat cattacttgg taacccaggt aaagcaattg caatcgtatt
    25441 gcttgtatta caaattgcag gtggtggagg aacattccca attcaaacta cgccacaatt
    25501 tttccaaaac atttcgccat acttaccatt tacgtatgca attgattcat tacgtgaaac
    25561 agtaggcggt attgttccgg aaatcctaat tacaaaatta attatattaa cgttatttgg
    25621 cataggattc ttcgttgtag gtttaatttt aaaacctgta acagatccat tgatgaagcg
    25681 cgtatctgaa aaagttgacc aaagtaacgt tacagaataa aaattaaatc cacacattag
    25741 ggttatagct ccttaatgtg tggattttta tgtttttaga cagaagagat agtaatttct
    25801 gtcttttatg ggacgggtgt tatcattgct attatccagg atgacttact atagcactaa
    25861 tattaccgac aaagtgaata tcctcgtctt ccgtagttaa aataaagcta gaaccttttt
    25921 ggatgtcata gtgcttatcg tttactgtta aagtaccagt accatcgata atagtaacta
    25981 agcaataagc atgtggttta ttgaatttta aatctccatg aatatcccat ttatatactg
    26041 caaaatattg attatctaca aattgagtta cagtgtgtgt gtcgatgtga gttgttatag
    26101 gagtagtatt tggttcatga ttgcctaatt caatcacatc tttactttgc tctaagtgca
    26161 aatcacgcaa ttgaccattt tgatctcgtc tatcatagtc ataaatacgg tatgtcgtat
    26221 cggatgattg ttgtgtctct aaaattaaaa tacccgaacc aatggcatgg acagtgccag
    26281 caggaacata ataaaagtca ccgggcttaa caggtatacg tttgaaaaga ctgtcaaatt
    26341 catgattatc aatcatgtcg attaacgtct gtttattatg tgcatgtacg ccatatataa
    26401 tttcagcacc tggggctgca tctaaaatat accaacattc tgttttacct agttcgcctt
    26461 cgtgttttaa agcgtagtca tcatctggat gaacttgaac agataattta tcattggcat
    26521 ctaatacttt agttagcaga gggaaactat ctcgtgaatc attatcgaat aattcacgat
    26581 gttgtgacca aagttgatct agggtcatat ccttgtatgg accattgata attgtattag
    26641 gaccatttgg atgtgcagaa attgcccagc attcaccagt tgtttcatta gggatatcat
    26701 agttaaatgc ttttaatgca tgaccgcccc aaattctgtc tttaaaaacg ggttgtaaaa
    26761 ataatgccat agttaaaact cctctatatt ttcattaata agttataaat ttctgtagta
    26821 ctgtttgcat taattagtga ttggcgtgtc tcatcattca ttaacgcttt agataagcgc
    26881 tgaagtattt ttaaatgtgt atcctgactg ttgtttggta cggcaattaa gaatatcaat
    26941 tgaggtagac taccatctag actctcccat ttaacaccat gattattttt cataacagct
    27001 acaatcggtt gttttacaac atcagacttt gcatgtggaa tggccacgtt catgccaata
    27061 gctgtcgtag actccatttc acgttctagg attgcatttt ttaaatgcga tgtgtgctct
    27121 acataacggc aaattttaag tttatgaatc aacatatcaa ttgcttcgtt tcgagacatg
    27181 tcgtgatcag taattatcat agtttgttga tcaaaaacat gagaaggttt attgagatct
    27241 gaatgtttcg cggtgttatc tacattgtca acctctgtat catgttgtgt aatatctgta
    27301 tcatgaagtt gcgtgtgttg cgctggtgca tctactgcta taactggtgt attgcgtttt
    27361 aataatagta cagtagtcat tgtgacaaga ctacctacta tcactgcaaa gataaaccat
    27421 aatacatgat caataccacc taatacagcc acgattggac ctccatgtgc gactctatcg
    27481 ccgacaccac caatggctgc aatgactgat gcaatcattg caccaatgat gtttgcaggt
    27541 ataatgcgca atggatcttg ggctgcgaaa ggaatagcac cttcagtaat accaaacagt
    27601 cccatagtga aggaagcctt acccatttct ctttcggaat gattgaattt atacttttga
    27661 acaaacgttg ctaaacctaa accgattggt ggtgtacata cagcaactgc gaccataccc
    27721 ataacggcgt aattaccttc agcaataagt gctgagccaa ataaaaatgc tactttgttt
    27781 actggaccgc ccatatcgaa ggcaatcatc gcacctataa tcatcgcaag tataataata
    27841 ttagcacctt gcatactttt taaccaggtt gttaatgcct caaaaatatt agaaattggt
    27901 gcaccgatta aaaatataaa tatcaatcct acaacgaccg atgaaataat gggaataata
    27961 atgataggca taattggtgc cattgctttt ggaactttaa tatctttaat ccactttgcg
    28021 atataacctg ctaagaaacc agcaacaata ccacctaaaa atcctgcgcc tgcatcactg
    28081 ccataaaaac taccgtcagc agcgatagcg ccgccaatca taccaggaac aagaccgggc
    28141 ttgtcagcga tactaacagc gatatatcca gctagtattg gaaccataaa tttaaaggct
    28201 aaactaccaa tgttttcaat ggatttccaa aatgaatcat ctgggatgac taatcctttt
    28261 gatgtcgttt caccgcctag agtcagcgcg atggcgataa ggagtccacc aactacgata
    28321 aaaggaacca taaacgatac accgttcatt aaatgttgat acaccatttg aataccactt
    28381 ttagacttac cgcgatcttt cgaatgataa tttgtttcag attgataaat aggcgcatct
    28441 tgattaatga tacgttgaat tagacctctc ggattatgaa tcccttcgcg aacattttca
    28501 ttaatcaacc gtttaccaac aaatcgggac agatcaactt gtttatcagc tgcaattatg
    28561 acaccgtcag cttcttcgat gtcttgcgta gttaaaacat tttcagcacc aacaccgccc
    28621 tgtgtctcta ctttaatatc cacacccatt tcttttgcta cctgctcaag cttttcttga
    28681 gccatatatg tatgtgcaat gccatttggg catgaggtaa tagctacaat tttcataaaa
    28741 tcatctcctt ttctatattg taagcgtatt ctcgatacta aaaaaaagaa taattaccgt
    28801 tactagtggc aattattctt gtaagtattc aaataactgt tgctttaaac tatgatcatc
    28861 taaactacat aaatggttca ctgaatcatc atccaagtta gcaattaatt gcatcatttg
    28921 ttttgtaaaa gctttgtctt tatgcgaaat cgctaagaaa aagacaagtt tgacatcgtg
    28981 ttgtcgccaa ggaaaaacat cttttgtgcg aaaaataagc acatgtgatt gtaaaacttt
    29041 ttcaggatct ccatgaggaa tcgccataaa attacctatg tatgtagaag atgatttctc
    29101 acgctctaaa gctgattcga tatatccttc tacaatcgca tgatgtgctt gtaatatttt
    29161 ttgagcttct tcaaaaattt gcacagtatg ccgtgatttt tgttcagtat ttacgacaag
    29221 gaaattgaca gtgtccatat gatgatgtgc ttgaactgga ttttgctttt gcttcacaac
    29281 gagtctgatt ttgtgacggt catcttcaga aaataatggt gcaaccttga tagtcgttag
    29341 gtgcttagga agtatgttta gcgtttgttt tggaatatca tgggtcgtta ttaataaatc
    29401 tacattgtca aagtgatagt gtgttatatt ttctagttta atcgtattta tcactgacaa
    29461 ctcttcggat aagttattta ttttagtttc taaaaaattc gacacaccta gaccataata
    29521 acaagcaatg actacattta attgtgtttt ggtacgacgc tcgatggcag cttgaaaatg
    29581 aattgttaaa aatgcaattt catcttcgct catctctata tcagtatcaa ttgctaattt
    29641 atcaatcgct tcaaaaagtg tgttaaacac aaagggatag agttttttaa tctctataac
    29701 taaaggattg tttaaataaa tgttttgagt gatacgtaaa tatgctttac taaaatgatt
    29761 atataaattt tgttgtaaaa tcgaatcttc attgaaaggt atatgaatac gttgctgcat
    29821 caattcgatt aagcgatcaa tataactttg tataaatata cgttctatgc caatatcgag
    29881 tttattaaaa tgataagcaa taaagaatga aaacatattg attacttttt cgttcaagtc
    29941 ataacctaat ctttcgttga tttgcttaat gcaagattga gatatcaatt ttgaatcgtg
    30001 gtttaacgtg tctagctcac ttaaagattg cttacgttta atgattaaaa tgagatgaat
    30061 taaaagctgt tgtatttgaa tatcagttgt ttcaatacta tgttgttgaa gtgtctcttg
    30121 tataatatgc gaaatcatcc tttggtgtga atcaggtaat tcatttaaaa ttaggtcttc
    30181 aacatgtaca tgccctgatg ataattgatt taaatggatg atggcattag tgatatcatt
    30241 atctgttcca tcgaccacaa taccttgttt tttcttgatt gttaaagcga tattaaattt
    30301 ttgacaccat tgttgaattc gaacgaaata gtcgttgagc acagttttag ataatagata
    30361 atcttgctct agttgttgca atgtctgcga tttattgtgc attagcagtt gatacgccat
    30421 agataataat agtttttctt tatatgattg aatatgcgtg taacgttcag ttatcgtttc
    30481 taaggtatac tgcgatgtat ttaattgata tcctttagat ttaatactaa cgatgatatc
    30541 atccataaaa ttgctattga taacatgaat atcattgcga actgtgcggt ttgaaacatt
    30601 gacatgttca gcaatttcat tagaactaat gtgctttgat ggatttttaa taaaaaactg
    30661 gagtagtttt aagtgtctat caagcattta acatgtacct cctttctaaa ttttccgtgt
    30721 aagcgttttt aaaggtgtat taatattatt aaacatgaga gcttatacat acgtcaatga
    30781 cattaaagcg aacttttata tgaatttaac agagtgcgaa ttatgcaaat aaagaacagc
    30841 agtaagatat ttcaaataga aaaatatcat attgctgttc ttctgaattt atgtacctga
    30901 tacttgtgga cgtatcaggt aaatgattaa tttttaggtg attgtgcttg aggtgtttgt
    30961 ttagaagacg tattggtatt atttgattta ttagcaggtg gtgtgttctg ttgttgatta
    31021 ttttggttgt ttgttgacga ttgactacca ccatcgtgag tattattttt atttgaattt
    31081 gagtcttttg gctgagctgg ttgcgtattc gatggtgctg ttgaatggtt gttaggaaca
    31141 tttgactgtg aagatggttg attgtcatta gtaacgttat tgccattatt ttgattttta
    31201 tctgctttgt ctgcactatc atcttgttga tcattttctt cggtttctgt ctttttatgc
    31261 gtagctttat tttcttgttc ttggttatta cttgatgatg tagaatgatt tattgcagca
    31321 taaattgcga acccaagtgc tccgattaat acgataatta cgatggtaat aagaagtttt
    31381 ttcaaatata acgcctccat gttaatgaaa agtagttgat acgtgaagct aaataattta
    31441 tttcaatatt gccataatca ttttaaaaaa taaatattag aaaagtaggc gatattaata
    31501 ttttcattgt tttttgtgta cttcaattag tataacaaaa ctgttatgta tttataaata
    31561 taatcattag tatttttatg gctgaaaaag ttataataaa agtgtaagga ataaaatatt
    31621 tgtatggaaa agagagataa ttatgaataa aaccagtaaa gtttgtgtag cagcaacatt
    31681 agcattgggc acactgattg gagtaacagt tgttgaaaat tcggcgccaa caagtaaaca
    31741 ggcacaggca gcaataaccc catattatac ttataatggt tatattggta ataatgctaa
    31801 ttttattctg gataagaatt ttattaacgc gattaagtat gataatgtga aattcaatgg
    31861 tattaaatta gctaaaacga atacgataaa aaaagtagag aaatatgatc aaacttttaa
    31921 aggtgtttct gcaaaaggaa acgaagcaag tcaattgcaa tttgtagtta aaaataatat
    31981 ttcattaaaa gatatccaaa aagcttatgg caaggacttg aaaaaagaaa atggtaaaac
    32041 aaaggaagct gatagcggta ttttttacta tcaaaatgct aaaaagacat taggcatctg
    32101 gtttgtcgtt gatcataata gagttgtcga agtaacagtt ggacatacac catacaaaac
    32161 aagtaaataa aataatggca tattaaggct agagtgtgag gagagatacc gcattctagt
    32221 cttttttatt aaataataac gattattgcg tcttacatag ttgtttgaaa ttgataaaaa
    32281 tgtttattt
//
LOCUS       DAHHYJ010000014        31832 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000014 DAHHYJ010000000
VERSION     DAHHYJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 31832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31832
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.014"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            245..1912
                     /locus_tag="NBK22_000490"
     CDS             245..1912
                     /locus_tag="NBK22_000490"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCD2413698.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(2158..2247)
                     /locus_tag="NBK22_000491"
     CDS             complement(2158..2247)
                     /locus_tag="NBK22_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413699.1"
                     /translation="MLLTTPIRLYLIDNNYQYQLLVLIVAHPN"
     gene            2246..4933
                     /gene="harA"
                     /locus_tag="NBK22_000492"
     CDS             2246..4933
                     /gene="harA"
                     /locus_tag="NBK22_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCD2413700.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDTNDAVVTNDQSSSVASNQTNTNTSNQNTSTINNANNQPQATTN
                     MSQPAQPKSSTNADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEYTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDINTKDDDTSQNNTSEPLNVQTGQEDKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDMVKDTDSSVDKDAHLNIDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPSDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            5064..5156
                     /locus_tag="NBK22_000493"
     CDS             5064..5156
                     /locus_tag="NBK22_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413701.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(5136..6041)
                     /locus_tag="NBK22_000494"
     CDS             complement(5136..6041)
                     /locus_tag="NBK22_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD2413702.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            6423..7685
                     /locus_tag="NBK22_000495"
     CDS             6423..7685
                     /locus_tag="NBK22_000495"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCD2413703.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            complement(7779..9053)
                     /locus_tag="NBK22_000496"
     CDS             complement(7779..9053)
                     /locus_tag="NBK22_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCD2413704.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            9225..9842
                     /locus_tag="NBK22_000497"
     CDS             9225..9842
                     /locus_tag="NBK22_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCD2413705.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            9970..11436
                     /locus_tag="NBK22_000498"
     CDS             9970..11436
                     /locus_tag="NBK22_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCD2413706.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWILKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            11543..12673
                     /locus_tag="NBK22_000499"
     CDS             11543..12673
                     /locus_tag="NBK22_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD2413707.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRSVLDNL"
     gene            complement(12634..12729)
                     /locus_tag="NBK22_000500"
     CDS             complement(12634..12729)
                     /locus_tag="NBK22_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413708.1"
                     /translation="MKPLVQSFTSSYAVKQQSNYKLSSTLLSSPK"
     gene            complement(12823..14427)
                     /locus_tag="NBK22_000501"
     CDS             complement(12823..14427)
                     /locus_tag="NBK22_000501"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD2413709.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEQFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGPRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            complement(14414..15574)
                     /locus_tag="NBK22_000502"
     CDS             complement(14414..15574)
                     /locus_tag="NBK22_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCD2413710.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLDVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            complement(15688..16134)
                     /locus_tag="NBK22_000503"
     CDS             complement(15688..16134)
                     /locus_tag="NBK22_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCD2413711.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPQI
                     QIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPSIQAK"
     gene            16214..16957
                     /locus_tag="NBK22_000504"
     CDS             16214..16957
                     /locus_tag="NBK22_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD2413712.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRISDYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRNYWYKKPNFSRIAQIASYVNPNYALVTRKFVDKAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            complement(17173..17775)
                     /gene="rpsD"
                     /locus_tag="NBK22_000505"
     CDS             complement(17173..17775)
                     /gene="rpsD"
                     /locus_tag="NBK22_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCD2413713.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(18019..18483)
                     /locus_tag="NBK22_000506"
     CDS             complement(18019..18483)
                     /locus_tag="NBK22_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCD2413714.1"
                     /translation="MTTINPTNYTLLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVGFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            18620..20314
                     /gene="ezrA"
                     /locus_tag="NBK22_000507"
     CDS             18620..20314
                     /gene="ezrA"
                     /locus_tag="NBK22_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCD2413715.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     SQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            complement(20388..20528)
                     /locus_tag="NBK22_000508"
     CDS             complement(20388..20528)
                     /locus_tag="NBK22_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413716.1"
                     /translation="MIMLFSLLILIKTTHLITFIFKQLLFIFIVPFIYPYKKEARQRI
                     VG"
     gene            20677..21825
                     /locus_tag="NBK22_000509"
     CDS             20677..21825
                     /locus_tag="NBK22_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD2413717.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKTQINAMINSKTNYDVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVKYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAKVIKNYPKAHFHVDAVQAFGKISMDLNNIDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            21825..23048
                     /gene="thiI"
                     /locus_tag="NBK22_000510"
     CDS             21825..23048
                     /gene="thiI"
                     /locus_tag="NBK22_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCD2413718.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAISTAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGAVLKHFDNISVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            23092..23862
                     /locus_tag="NBK22_000511"
     CDS             23092..23862
                     /locus_tag="NBK22_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCD2413719.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLFVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLIFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMAISMIAGSY
                     AGAHFAIKQGVGYVKVLFIIITAILILKNAFDYIQQFV"
     gene            23960..24454
                     /gene="tpx"
                     /locus_tag="NBK22_000512"
     CDS             23960..24454
                     /gene="tpx"
                     /locus_tag="NBK22_000512"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCD2413720.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSDASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     YKNI"
     gene            24577..25524
                     /locus_tag="NBK22_000513"
     CDS             24577..25524
                     /locus_tag="NBK22_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2413721.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINVLKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            25612..26814
                     /locus_tag="NBK22_000514"
     CDS             25612..26814
                     /locus_tag="NBK22_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCD2413722.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLIERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(27060..27560)
                     /locus_tag="NBK22_000515"
     CDS             complement(27060..27560)
                     /locus_tag="NBK22_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD2413723.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            27701..28819
                     /gene="ald"
                     /locus_tag="NBK22_000516"
     CDS             27701..28819
                     /gene="ald"
                     /locus_tag="NBK22_000516"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCD2413724.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            28943..29041
                     /locus_tag="NBK22_000517"
     CDS             28943..29041
                     /locus_tag="NBK22_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413725.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLG"
     gene            complement(29135..30190)
                     /locus_tag="NBK22_000518"
     CDS             complement(29135..30190)
                     /locus_tag="NBK22_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD2413726.1"
                     /translation="MTKISKIIDELNNQQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLTSGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKEAQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIL
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            30365..31054
                     /locus_tag="NBK22_000519"
     CDS             30365..31054
                     /locus_tag="NBK22_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD2413727.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKVNFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(31359..31772)
                     /locus_tag="NBK22_000520"
     CDS             complement(31359..31772)
                     /locus_tag="NBK22_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD2413728.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
BASE COUNT        10959 a         4673 c         5746 g        10454 t
ORIGIN      
        1 aatatgatct ttggaagatg attattttaa ataatagaaa aatatagtta tcaatagtat
       61 caagcgctaa aagttgtata atgcaaaact ttaataagtg aatttattgc aaaaatgaaa
      121 gcgctaaccc gatttagtcg acaagttttt aacagttcgt tattatatga atgtaagtaa
      181 aaatttctta gctacaactt acatatttaa aatgcataaa ttaaacaaaa aggggcgaaa
      241 aaagttgact catttatcag atttagatat tgcgaatcaa tcaacactac aaccaattaa
      301 ggatattgct gcatcagtag gtatttcaga ggatgcatta gaaccttatg gtcattacaa
      361 agctaaaatc gacattaata aaataacgcc aagagaaaac aaagggaaag ttgttttagt
      421 aactgcgatg agcccaacac cagctggtga aggtaaatca acggttacag ttggtttagc
      481 tgatgcattc catgagttaa ataaaaacgt tatggttgca ttaagagagc ctgctttagg
      541 accaacattt ggtatcaaag gtggtgcgac tggtggtggt tatgcgcaag tcttacctat
      601 ggaagatatc aacttacatt tcaacggaga tttccatgcg attacaactg caaataatgc
      661 attgtctgcg tttatcgata atcatattca ccaaggtaat gaattaggaa tcgatcaaag
      721 acgtattgaa tggaaacgtg tattagatat gaatgatcgt gcacttagac atgtaaacgt
      781 tgggttaggt gggcctacaa atggtgtacc acgtgaagat ggctttaata ttacagtagc
      841 gtctgaaatt atggcgattt tatgtttaag tagaagtatt aaagacttaa aagataaaat
      901 tagtcgtatt actattggtt acactagaga tcgcaagcca gttacagttg cagatttaaa
      961 agtggaaggt gcacttgcaa tgattttaaa agatgcaatt aaaccaaact tagtacaatc
     1021 aattgaaggg acacctgcat tagttcatgg tggaccattt gcgaatatcg cacacggttg
     1081 taactcaatt ttagcaactg aaacagcacg tgatttagct gatatcgttg taacggaagc
     1141 tggatttggt tcagacttag gcgctgaaaa attcatggat attaaagcgc gtgaagcagg
     1201 atttgatccg gcagctgtcg ttgttgttgc gacaattcgt gcgttaaaaa tgcatggtgg
     1261 tgtagcgaaa gataatttaa aagaagaaaa tgtagaagca gtaaaagcag gaattgttaa
     1321 tttagagcgt catgttaata atattaaaaa attcggtgta gaaccggttg ttgcaattaa
     1381 tgcatttata catgatactg atgcagaagt agaatatgta aaatcttggg ctaaagaaaa
     1441 taatgtacga attgccttaa ctgaagtttg ggaaaaaggt ggtaaaggtg gcgttgactt
     1501 agcaaatgaa gtattagaag tcattgatca acctaattca tttaaacctt tatatgaatt
     1561 agaattacca ttagagcaaa agattgaaaa gattgtgact gaaatctatg gcggttcaaa
     1621 agtaacgttt agcagtaaag cgcaaaaaca attaaaacaa tttaaagaaa atggttggga
     1681 taattaccca gtatgtatgg cgaaaacaca atattcattc tcagatgatc aaacgttgtt
     1741 aggtgcacca tcaggatttg aaattacaat tcgtgaatta gaagcgaaaa caggtgcagg
     1801 atttatcgta gcgttgacag gtgcaatcat gactatgcct ggtttaccta aaaaaccagc
     1861 agcattaaac atggatgtta ctgatgatgg tcatgcaatt gggttattct aataaatcat
     1921 gtcaattgtt taataaagat aagtaaatag tttaatagac cggactgttg gagatgcatt
     1981 atttcagcag ttcggttttt tgctgtgcta aaaatagatt caatttggcg aatctaacga
     2041 caatgtttga aggtggttaa ttaatgtata tgaagataaa aagtgggctt gaagaatagg
     2101 aaagcgatgc aatgaatatt acatattaaa aaaagttaat aaaataggtt gcaatattta
     2161 attgggatgc gctacaatta acactaataa ttgatattga taattattat caattaaata
     2221 taatcttata ggagttgtta acaacatgaa caaacatcac ccaaaattaa ggtctttcta
     2281 ttctattaga aaatcaactc taggcgttgc atcggtcatt gtcagtacac tatttttaat
     2341 tacttctcaa catcaagcgc aagcagcaga aaatacaaat acttcagata aaatctcgga
     2401 aaatcaaaat aataatgcaa ctacaactca gccaccaaag gatacaaatc aaacacaacc
     2461 tgctacgcaa ccagcaaaca ctgcgaaaaa ctatcctgca gcggatgaat cacttaaaga
     2521 tgcaattaaa gatcctgcat tagaaaataa agaacatgat ataggtccaa gagaacaagt
     2581 caatttccaa ttattagata aaaacaatga aacgcagtac tatcactttt tcagcatcaa
     2641 agatccagca gatgtgtatt acactaaaaa gaaagcagaa gttgaattag acatcaatac
     2701 tgcttcaaca tggaaaaagt ttgaagtcta tgaaaacaat caaaaattgc cagtgagact
     2761 tgtatcatat agtcctgtac cagaagacca tgcctatatt cgattcccag tttcagatgg
     2821 cacacaagaa ttgaaaattg tttcttcgac tcaaattgat gatggagaag aaacaaatta
     2881 tgattatact aaattagtat ttgctaaacc tatttataac gatccttcac ttgtaaaatc
     2941 agatacaaat gatgcagtag taacgaatga tcaatcaagt tcagtcgcaa gtaatcaaac
     3001 aaacacgaat acatctaatc aaaatacatc aacgatcaac aatgctaata atcaaccgca
     3061 ggcaacgacc aatatgagtc aacctgcaca accaaaatcg tcaacgaatg cagatcaagc
     3121 gtcaagccaa ccagctcatg aaacaaattc taatggtaat actaacgata aaacgaatga
     3181 gtcaagtaat cagtcggatg ttaatcaaca gtatccacca gcagatgaat cactacaaga
     3241 tgcaattaaa aacccggcta tcatcgataa agaatataca gctgataatt ggcgaccaat
     3301 tgattttcaa atgaaaaatg ataaaggtga aagacagttc tatcattatg ctagtactgt
     3361 tgaaccagca actgtcattt ttacaaaaac aggaccaata attgaattag gtttaaagac
     3421 agcttcaaca tggaagaaat ttgaagttta tgaaggtgac aaaaagttac cagtcgaatt
     3481 agtatcatat gattctgata aagattatgc ctatattcgt ttcccagtat ctaatggtac
     3541 gagagaagtt aaaattgtgt catctattga atatggtgag aacatccatg aagactatga
     3601 ttatacgcta atggtctttg cacagcctat tactaataac ccagacgact atgtggatga
     3661 agaaacatac aatttacaaa aattattagc tccgtatcac aaagctaaaa cgttagaaag
     3721 acaagtttat gaattagaaa aattacaaga gaaattgcca gaaaaatata aggcggaata
     3781 taaaaagaaa ttagatcaaa ctagagtaga gttagctgat caagttaaat cagcagtgac
     3841 ggaatttgaa aatgttacac ctacaaatga tcaattaaca gatttacaag aagcgcattt
     3901 tgttgttttt gaaagtgaag aaaatagtga gtcagttatg gacggctttg ttgaacatcc
     3961 attctataca gcaactttaa atggtcaaaa atatgtagtg atgaaaacaa aggatgacag
     4021 ttactggaaa gatttaattg tagaaggtaa acgtgtcact actgtttcta aagatcctaa
     4081 aaataattct agaacgctga ttttcccata tatacctgac aaagcagttt acaatgcgat
     4141 tgttaaagtc gttgtggcaa acattggtta tgaaggtcaa tatcatgtca gaattataaa
     4201 tcaggatatc aatacaaaag atgatgatac atcacaaaat aacacgagtg aaccgctaaa
     4261 tgtacaaaca ggacaagaag ataaggttgc tgatacagat gtagctgaaa atagcagcac
     4321 tgcaacaaat cctaaagatg cgtctgataa agcagatgtg atagaaccag agtctgacat
     4381 ggttaaagat actgatagta gtgttgacaa agatgcgcat ctaaatattg atcatttatc
     4441 cgatatgtcg gataacaatc acttcgataa gtatgattta aaagaaatgg atactcaaat
     4501 tgccaaagac actgatagaa atgtggataa agatgccgat aatagcgttg gtatgtcatc
     4561 taatgtcgat actgataaag actctaataa aaataaagac aaagtcatac agctgaatca
     4621 tattgccgat aaaaataatc atactggaaa agcagcaaag cttgacgtag tgaaacaaaa
     4681 ttataataat acagacaaag ttactgacaa aaaaacaact gaacatctgc cgagtgatat
     4741 tcataaaact gtagataaaa cagtgaaaac aaaagaaaaa gccggcacac catcgaaaga
     4801 aaacaaactt agtcaatcta aaatgctacc aaaaactgga gaaacaactt caagccaatc
     4861 atggtggggc ttatatgcgt tattaggtat gttagcttta ttcattccta aattcagaaa
     4921 agaatctaaa taattaactg gatatagcat atgtatgatt aactttgtag acaatgtgaa
     4981 accaattaaa ttataaacta ttgattggtt taatggcttt cctttagagt aaataaaaag
     5041 aacagcagtg agaaattttc taattgaaaa taatcttact gctgttttta atatttggat
     5101 tcattgttgt ggttacttta aaaagtgagc atcaattaac gcttttttcg atttaacaaa
     5161 tgtgatttaa tatcatattt taatgcgtcg ttgtattctt tttcagtgat ttgatcttcg
     5221 attaacatac gctttaatac ataatgttgt ctttggatac tatatttcaa atctttatcc
     5281 gattttaatg ttccatcttt ttcgtagggt gtatagccat aagggctttg caacaaaccg
     5341 ataaggtatg cagattgtgc aattgataaa tcttttggtg gaataccaaa caaactatat
     5401 gaagcggatg caattccgga aatattagcg ccattataat ctctaccgaa gggaactata
     5461 tttaaatatg tatatataat ttcatctttt gagagtaggt gttctaatct aattgctagg
     5521 cgaagttcat ttgcttttct actatatgtt ttttcgttgg taagaacttg atttttaaca
     5581 agttgttgtg taattgtgct accacctgaa ctttgatcag tattaaaaat atcttgtatc
     5641 attgctctta aaatcgcctt tggtaagatg ccatcatgtt tataaaataa agtgtcttca
     5701 gatgacgtta atgctttaat gacatttgga cttgatgttt tagggcctat aatgagtgag
     5761 ttttgagaat ggttatactc atataataaa tttttgttat tatgatctaa taattcatcg
     5821 ccaggtattt gtcgaacttt ttttattaaa gcatcatctg ataatgagtc ggacgtttta
     5881 gttaaatgat gaaaatataa agacatcgca atcacagcga tagcaacgat ggcaatgaaa
     5941 atcataaata taaaaacaat gacatgtttg atagctttat atatcttttc aaaatgatat
     6001 atacgatgat tcaattgatg attgttgtct tgattcgtca tctttaacct cctaaattac
     6061 gtatagtata ccatatttaa gcaatttgac ttttatttta acgttaatta taattaggga
     6121 aaatgcataa agaataggat aaaaggacaa gtaatattat ttgaaatcat cagagagcta
     6181 gtggtcgctg tgaactagtg tttttaataa tatgaataca cctttataac taacatgtcc
     6241 tacgtttatt aatgaacgta tcattaatag agtgaacatt atcttatgat tatgtttaag
     6301 tttggtggta acgtgcaatc gcgcccttac atatttgttg taagggtttt ttttacgttc
     6361 aaaaaacttt aaagtatttt aacaagtatt caataaataa ttataaatag gaggaaataa
     6421 taatgacgaa tgtattaatt gaagatttaa aatggagagg tcttatttat caacaaactg
     6481 atgaacaagg tattgaagat ttattaaata aagaacaagt gacgttatac tgcggtgccg
     6541 atccaacggc agatagttta catattggtc acttactacc attcttaaca ttaagacgtt
     6601 ttcaagaaca tggacatcgt cctatcgttt taattggcgg tggtacaggt atgattggtg
     6661 atccatcagg taaatcagaa gaacgtgtgc tacaaacaga agaacaagta gataaaaata
     6721 tcgaaggtat tagtaagcaa atgcacaata tttttgaatt tggaacagac catggtgcag
     6781 tgcttgttaa taatagagac tggttaggac aaatctcatt aattagtttt ttacgtgact
     6841 atggtaaaca cgtcggcgtt aattacatgt taggtaaaga ttcaatccaa agtcgtttag
     6901 aacatggtat ttcatataca gaattcacat acacgatttt acaagctatt gatttcggtc
     6961 atttgaatag agaattgaat tgtaagattc aagtaggtgg atcagatcaa tggggtaata
     7021 tcacaagtgg tattgaatta atgcgtcgta tgtatggtca aacagacgca tacggtttaa
     7081 ctattccgct tgtaactaaa tcagatggta agaaatttgg taagtctgag tcaggtgctg
     7141 tttggttaga tgctgaaaaa acaagtcctt atgaatttta tcaattctgg attaatcaat
     7201 cagacgaaga tgtaattaaa ttcttaaaat actttacttt cttaggaaaa gaagaaattg
     7261 atcgcttaga acaatctaaa aatgaagcac cgcatttacg tgaagctcaa aaaacattag
     7321 ctgaagaagt aactaaattt attcatggtg aagatgcatt aaatgatgca atccgtattt
     7381 cacaagcatt atttagtggt gatttaaaat cattatcagc gaaagaatta aaagatggat
     7441 ttaaagatgt gcctcaagtg acattatcaa atgacacaac aaatatcgtt gaagtcctta
     7501 ttgaaacagg catttctcct tctaaacgac aagcacgtga agatgttaac aatggtgcga
     7561 tttatattaa tggtgagaga caacaagatg ttaattatgc tttagcacca gaagataaaa
     7621 ttgatggcga atttacgatt attcgtcgcg gtaagaaaaa atacttcatg gttaactatc
     7681 aataaatata attgcatagc taaataaatt agagcctact catattcatt cctaagaatg
     7741 taatgagtag gctcttaatg tacttttctg tctgtaaatt atctaaagaa atctctatcg
     7801 ccttggccag gtgattgacg ttctgatcgg ctttgacgtt ttggttgttc tttttgttgt
     7861 tttaatttca ctttaacttc ttttgtttta ccatcacgga taacggtaac agtgactgat
     7921 tcaccaggtt ttttattttc atataaatag cttcttaaat caacatcatc tttaattttc
     7981 ttgccatcaa tttctgtaat aatatcacct tttttaagat caatatcact atcagctttg
     8041 gcgacataaa taccgtcttc tctatcagta tgaagttgct cgcgctcttc ttcaggaata
     8101 tctttcaaat taattaaacc aataccaatc gaagggcggt caattttacc atgttttaca
     8161 agttgttcaa ttgttacttt aacttcatta cttggaatag caaacccgat accttcaact
     8221 tgtgtcgcag caattttcat tgagttaata ccaactaaat taccattaat atctactaat
     8281 gcgccacctg agttacctgg gttaatagca gcatctgttt gaagaacgct aacttttgta
     8341 ttgccaccag ttgtctcagc gtcaatcgta cgttcgcttg ctgaaatgat accagatgtt
     8401 acagagttag caaattgtaa tcctaatggg ttacccattg cgaatacgct atcgccagtt
     8461 tgtacttttg aagagttggc aaattgaatc gctttaatac cttttgtatt ttcaatttta
     8521 agtacagcaa tatcagttac tgcatcttta ccaactaatt tcgctttaac ttgtttttta
     8581 ttatgtaatt ggactctaat ttcatttgcg ccatcaataa catgattgtt tgtaacgata
     8641 taagctgaat tgttgtttat ttgatagata acacctgaac ctactccagc ttcagatggt
     8701 ttagatgatt tgccttttaa taagtcgtct acacttgatg ctttttgcat gttaataact
     8761 ccaacaattg taggggagac agattttatc atttcatgaa cggtaccgaa tttcttgctt
     8821 tgaccgtcta attgattgcc acctttatta tttgttgtct gaacagttga accatcttta
     8881 tttaaaattg tactatttaa tactttgcct ataccaagta ctagaagtgc accaataatt
     8941 ccagcaatca atgcaacgat gactgtttta aaccatggaa atttaggtct tctgtatcta
     9001 ggtgtttggc tatggtttgt tgtagaatga tctgtatgat taaaatctga catacttaac
     9061 ctccattata tgatttatat atgctttaat tatgtctttt ttttataatt attgctagta
     9121 atacactata caactttaga tgtattttca gactatcttg ttctagtaaa aatatagtga
     9181 taaaatctag ttgttataaa caaataagtt aagtaggtgg cacaatgtat tcagtgatta
     9241 gtaagatttt gaatttcatt ttagttaaaa tgtcaaaatc attatacgta atcggtaaag
     9301 ataatattcc taaggatagt aaatatgtcg taacttgtac gcatgaaagt tataacgaag
     9361 tcattatgct aggtatggca ttatatccta atcaaattca ttatatggct aaaaaagaat
     9421 tattcaaaaa taaatggatt ggtaaatttt taacgtcttt aaatgcattt cctgtagatc
     9481 gtgaaaatcc aggacctagt actttaaaac gcccaatcaa tttgttgaaa gataataaga
     9541 cagttggtat cttcccaaca ggttctcgca catcgcaaga aggtgcacct ttgaaacgtg
     9601 gtgcttcaac aattgcaatg ttaagtaagt caccaatatt acctgttgct tatgttggtc
     9661 ctactaagat tcacggtctt ttaacaggac aagcatacat aaatattggt aaaccaatcg
     9721 atattaacga tttacctaaa gatttaaaac gaaacgagcg tatcgattat attacaaaag
     9781 agattgaaac acgaacagca aagttacaac aagaattgca tgaaattgta aaaagtttat
     9841 aaaataactt gaggtagaaa tatagctggt tagtaagcct aggtgactat aacggagtat
     9901 cgaagtagtt attctagtta cattagtaat ctttatattt ctgccttatt ttaagaagga
     9961 tgacattcaa tgtataaatt tttccaaaat ttagggcgtt cgttaatgtt accagttgcc
    10021 attttaccgg ctgcagcaat tattgccgga attggtaata cgttaaatgc acttcatgcg
    10081 acgccgaaaa tcgcaatgtt ctttactacg gtaggtacta cgattttaga gcaattaggt
    10141 attttatttg caattggtgt agctatagga atggcaaaga aaaatgatgg tgcagtcgcg
    10201 cttgcagcta cgttaggata ttttttagtt acagttgtat tatcaccaat gaaattagcg
    10261 cctttattag gaatgaaagc ttcggaaata aattcagctt ttgaaaagat gaataatggc
    10321 aatgtctttg ttggtattgt aataggttta atagcagctt atgcatacaa taagtttagt
    10381 gaaacagaat taccattagc attatcattt tttagtggta aacgtctagt tccaattatg
    10441 actgcatttt actgtacatt tttagttgtc atattgttat tcttatggcc actactttat
    10501 tcatggattt taaaatttgg tgaatctata gtcggtttag gatcgtttgg agctttcata
    10561 tatggtgttg ccaatagatt attaattcct acaggattac atcatgcact gaatagtgtg
    10621 ttctggtttg atacgattgg tattaatgat attggtaaat tccaaagtgg caaagatgct
    10681 ataaaaggta ttacaggacg ctatcaagcg ggattcttcc caatcatgat gtttggtatt
    10741 ccagcggctg cgttagcaat gtatcataca gctaaaacga ctcagaaaaa acaagtttac
    10801 ggttggttcc tagcgagttc agttgcagca tttttcgttg gtgttacaga gccgattgag
    10861 tttgcattta tgtttgttgc gccaatctta tatgttgttc atgcgttatt aacaggatta
    10921 tctttattta tcgcagctac attccattgg acagctggat tttcatttag tgcaggtttg
    10981 atagattatg tgttatcact aattaatccg gtatcaaatc atccattgat gttgcttgtt
    11041 caaggtgttg tatttttcat cttatactat gtgattttcc gtgtagtaat ccaagtattt
    11101 aacttgaata cgattggtag aggtgaaaat gaattagttg atccaacagt tgtaaaagat
    11161 aatattgctc ctggtgaaaa tgatattaaa caaagtaaat atcatcaaca tgctatacaa
    11221 atattagaag gtttaggtgg tcaagagaat attgttaatt taactaattg tgcaacaagg
    11281 ttgcgtctag agttaaaaga cacatctatt attgatcaac agaagattaa aaacgccggt
    11341 gcagttggtg ttactgttaa tggtaaacat tcaactcaag ttattgtcgg tacacacgta
    11401 cagcaggttg ctgatgaaat tgaaaaacat ttataaaatt taaacagcta aagcgcaaat
    11461 ccaacttcaa tagggtttgc gcttttttta gtcaaatagt agaatgattt atatctgaat
    11521 tttcagaaat ggagtgggga ttgtgaagaa aattttattt gatgttgatg gcgttttttt
    11581 aagtgaagaa cgttgtttcg atgtctctgc attaacggta tatgaattat taatggataa
    11641 gtgttattta ggtcttcatt cacatataga ttgggaaact ttgactgata acgatataca
    11701 agacattagg aatcgaattt ttcaaaaaga taagatttta aacaaattaa aatcgctagg
    11761 gctgaattca aattgggata tgttatttat cgtttttagt attcatttaa ttgatatttt
    11821 gaaaaagtta tcacatgatg aaattgaggc attcatgtat caagatgagc ctgtcgaatt
    11881 gaagttgcaa aatataagca caaacttagc agactgtttt aatttaaatg aacaactacc
    11941 tttacaattt ttggataatg taaaagttgg taaaaataat atttatgctg ctttggaaga
    12001 gtttgcaaca acagagttac atgtttcgga tgctacttta tttagtttga agggtgcatt
    12061 atggacgtta gcgcaagaag tttatcaaga atggtattta ggatcgaagt tgtatgaaga
    12121 tgttgaaaag aaaatagcac gaactacttt taagacaggt tatatttatc aagaaattat
    12181 tttgagacca gtagatgaag ttaaggtact tctgaatgat ttaaaaggtg ctggtttcga
    12241 attaggtatt gcaacaggtc gtccttatac tgagactgtt gtgccatttg aaaatttagg
    12301 attgttacca tattttgagg ctgattttat tgcaacagca agtgatgttt tagaagcaga
    12361 gaatatgtat ccgcaagcac gaccattagg aaagccgaat ccttttagtt atatcgcagc
    12421 tttatatggt aataatcgcg ataaatatga atcttatatc aataagcaag ataacattgt
    12481 aaataaagat gacgtattta tagtaggcga ttcgttagct gacttattaa gtgctcaaaa
    12541 aataggtgca acgtttattg gaacattaac aggtttaaaa ggtaaggatg ctgcaggtga
    12601 gttagaagcg catcatgccg actatgttat taatcattta ggtgaactta gaagtgtact
    12661 agataatttg taatttgatt gttgtttgac agcataactt gaagtgaatg attgaaccaa
    12721 aggtttcata ttgagttaca atgaaattaa taatgaaaaa atgccaagaa gcaatggaag
    12781 taatccaatg tcttcttggc attttgaatt tacataaatt gtttatgact gtaccgtcaa
    12841 ttcagttgtg aaaatttgat tgtattcacc aacttgttta agttcatcaa ttatattgtt
    12901 tgaaacaggt tgatcaacgg ataaaatcat tagcgcatct ccgcccgctt cagttctacc
    12961 taaagtcata gatgcaatgt tgatattgta tttacctaac aatgcgccag tttttcctac
    13021 catacctgga gtatcattat gatatgacac aatttgatat tgatttggct ttaagtctac
    13081 agaaaaatta ttaattctaa caattcttgg accgaaacct gtaaagacag aagcgccaac
    13141 tttaacggaa tcgctatcgt ttgatagttc tacctctaag tagttactaa aacctgtctc
    13201 tgctttatta ttttcaatat ttaatgtcac ttgttgttga tttaacaaca ttagagcatt
    13261 gataatattg acttcaggac caagatcatc ttttaaaata tgagtaataa ttgtacgtgt
    13321 taataaacta ctatcaatag aggctaagtc gccaccgtac gtaattttaa tagagcttgg
    13381 tgcattgtac attaattgaa tagctaattc accaactgtt tggcttaaat tgatgaatga
    13441 ttttacagta tcatctatat tgcttaagtc cattttaggt gcattcactg catgcgttac
    13501 agtaccatca attaaaattt cgatgatttc atttgaaaca gaaattgcca ctttttcttg
    13561 agcttcgact gttgatgcac ccaaatgagg tgtaacaata attttatcat gtgcaacaag
    13621 aggcgaatca gttgcaggtt catgttcaaa cacatcgata gctgcccgac taatttgtcc
    13681 ttcgtctaat gcttttatta gcgccttttc atcaataata ccaccacgtg ccacattgat
    13741 tatttgcaaa ctaggttttg ctttggcaaa aaagtcagca ttaattaagc cttttgtttt
    13801 aggtgttagt ggtgtatgta atgtaacgaa atcagaatgt tgggcaatct catcaactgt
    13861 tgccttcgta atgcttaaag attttgcttt ttcatccgtt aagtaagggt caaaagctag
    13921 tattttcatt ccgaaacttt gcgcacgttt agcaacacct aaaccaattc taccagcacc
    13981 aatgacacct aatgttttat gataaagctc agtaccttta aatgcatttc gattccattc
    14041 tttatttgta agtgattggt gtgcttgcgg aatatttcga gccattgata ataacattgc
    14101 cagtgaatgt tcagtagctg aaatcgtatt accatctggg gcattaataa ctaaaatacc
    14161 ttttaatgtt gcagcatcaa tattaatatt atctacacca acaccggcgc gtgcgattac
    14221 ttttaaagaa tcagcagcat ttatgatatt ttcagtgacc gtagtttgac tacgaacgat
    14281 taaagcatgg tatgaaggta taattttgat taatgcttct tcggacaagc cagtttgaat
    14341 atctacatta aattgttcgt gatctaataa tgctttgata ccatcttttg atatgggatc
    14401 tgcaacgagt acattaaatt gcttcatgaa taacctccat atattttgat atacctttac
    14461 cgatatagtt aactttacgg tgttcagtta aaataatttc taaagcagat actaccgata
    14521 aaatatcgaa aggactaatt ttccccatat gaccaattct taaaatttga cctttaagat
    14581 ggccttgacc accagcaatt gttattttaa accgattttt aagttcatct ttgattattt
    14641 tcacttcatc atttgtatta ggtttgaatg ctgtaaccgt tggagatgca tctttatcat
    14701 tgactaataa agttaaatct aatgctttta aggcgcttct taatgcattt ctaattgcat
    14761 agtgtcgtgc tattacgtga ttgaaacctt ctgcttttac ggtttcaacg tatgcattta
    14821 cacctctaaa taaagacaca tttggtgtga acggtgtaga attgtcagct tgtgacgaaa
    14881 tgtatttatt taagtctaga taaaattttg gcgtagttac ttctttgaaa cgttcttttg
    14941 cacggtggct ataagctaca aaagctaatc ctggaggtaa cataattgct ttttgactac
    15001 cagaaacaag tacatcaatt ttatctttgt taatgtcaac atcaacagca ccaatgcaac
    15061 ttacgccgtc aactacaaaa taaatattac tattaaattg attgatggca tttcctaact
    15121 cgtgaatagg gtgtagcact gtcgtagatg tttcgcaata ttgactaaat actgctttaa
    15181 cttcaacatc taaagttgaa agataattga tgaaatcttt aacatctaca gcttctcccc
    15241 atgttacgtc ataaatgtgc acatttttgt aataagtttg tgcaatttgt ttaaatcggt
    15301 taccaaaggc acctgaaaca atgacaacga agtgatcttc agggtttaca atgttcaaca
    15361 tactagcttc taagacgctt gtaccgctag atgttaaaat aagtacatca ttttgactcc
    15421 caaatattgg ctttagacct tgaaatgctt gttgtgcgat gtcttcaaaa tctttagaac
    15481 gatgaccaac cataggtgct tgaatttctc tcataatggc atcaggtaca ggggttgggc
    15541 caggtgttaa taacaacggt tgatgataat acatttatat gcctcctata aataagattt
    15601 cactatttta gcaaattttc tgaaaattta aaagcctaaa aaagtgataa cgctatattg
    15661 tcgaacaaac gttatcactt aacgatttta tttggcctga atgctgggat agatttttat
    15721 gtccacatta tttcttacag catttgaaat catgcaatta ttatctgcaa ttgttatcaa
    15781 ttttggtaat cgcttttcta attgtgctat ttgatcactt ggaatttgaa tttgaggatg
    15841 gtgcacaatt tttgacatac tgaattttcc gttatttaaa caagctgttc caatcgattg
    15901 ttgttcaatt gaaatatctg tgaactttgc acgttcaaga gtagctgcta atgagatgat
    15961 ataacatgat gaagcggctg atactaacat ttcatcggga tttgttccta taccaacacc
    16021 acctaaagaa gcaggtatag aaatattctc tgaaagtatg tcgccttgaa cgtttccgac
    16081 attgttacga ccaccttgcc aagaagtttg gactttaaag tcatgttgat gcaattgctt
    16141 taacctccaa tataattgtg atagtttaat tttagaatag tttatcaaag tttaacagaa
    16201 aggtgactta tcaatgactc tgaataaact gaaagatgaa ttacaaattg tttcgcaccg
    16261 tggattgccg agtgattttc ctgaaaatac aatggtcggt tatcgagagg taatggggct
    16321 caatgttgct atgttagaaa tagatgttca tttgaccaaa gaccaacatt ttgttgtgat
    16381 acatgatgaa acaattgata gaacatcgga tggtaagggg cgtatttctg attacacatt
    16441 atcgcaatta aaatcatttg attttggtag ttataaagat gttgctttta agggagaacg
    16501 aatacccacg ttagatgaag tactttcatt atgcttaaag tatgataaaa agctattgat
    16561 tgaattgaaa agtccgaatt tatatccaga aatagaatgt aaattgctgg catttttgga
    16621 agaaaagaaa gtcgatgcga cacaagtagt tatacaatct tttgatatcg aatgcatcga
    16681 aaagttaaac acattgggca gtatatatga attaggtgtg ctttgtagta aacgtaatta
    16741 ttggtataaa aagccaaact tttcaagaat tgctcaaatt gctagttatg ttaatcctaa
    16801 ttatgcatta gtgaccagaa aatttgttga taaagcacat catcatcaat tacaggttat
    16861 gccatacaca gttaataaat tgaaaactgg agaaaaatta agacaaatgg gtgtagatgg
    16921 attaattaca gataatccga aattatttat taagtgagac gatgaatggt aatttgatag
    16981 agggatgagg attcgtatat cgcaattgat aattttaaaa actaatgatg aaatactaaa
    17041 gataattaaa caaataagac ttgtaaattc taagttgatt gtaaaatagg gtagctccat
    17101 ataattttga atctatcatt tttaaattaa taaaaaaaac acccacaatt gtgggtgttt
    17161 gtattgaacg tattatcttg agtagtactc aacgattaat tgttcgttaa tttcagcagg
    17221 taattcgcta cgttctggta aacgtacgaa agtaccagtt aagctgtcag catcaaagtt
    17281 taagtactca ggtacgaaat tgttgatttc aactgattca acgatgatgt ttaatttttg
    17341 agatttttca cgaactgaaa ttgtttgacc aggtttaaca gaataagatg gaatatcaac
    17401 acgtttacca tctactaaga tatgaccgtg gttaactaat tgacgtgctt gacgacgagt
    17461 acgagctaaa cctaatgaat aaacaacagc gtctaaacga cttgctaata agatcatgaa
    17521 gttttcaccg tgtacaccga attttttacc agcgatgtca aatgtgttac ggaattgtct
    17581 ttcagtcatt ccatataagt aacgtaattt ttgtttttca cgtaattgta aaccatattc
    17641 tgataatttt ttacgttggt ttggaccatg ttgtcctggt gcgtaaggac gtttttctaa
    17701 ttctttacca gtaccgctta aagagatacc taaacgacga gattttttcc agtttgaacc
    17761 tctgaatcga gccataataa gactcctcct ttaattcttt tttgttgtta tgaataaaca
    17821 aaaaagagtg ttgcatgctt actaagatat attgttttat gtgtcttcac cttatagcga
    17881 cagttacgtg acacgtccgc attgggaaca acatagcgct caataatata caactgctac
    17941 tttcgttaat tcacacaaag attattgtaa cattttcctg ctaactgtca acactttatt
    18001 gaaaatgctg atgtcctttt atgcgagttg cttttcaata attttaacaa ttgcttcaag
    18061 atgttctttg tcattgtcat caaatcgatc cgttataggg gcatcgatat ctaagacgcc
    18121 gataatttta tcatctttga aaattggaac gacaatctca gacttactat tagcatcaca
    18181 agcgatatgt ccttcgaatt gatgaacatc agctacaatt tgtgtacgac gttctgaaac
    18241 ggctgtacca catacacctt ttccaattgg aatgtggaca caagcggggt gtccttggaa
    18301 aggtccaagt ataagttcat tttgttccaa taagtaaaag ccgacccaat taatttgatc
    18361 tagattatca tttaataagg cagacatatt acttaaaata gcaatcatat gatgttcatc
    18421 ttcaataagg cttgctgctt gtttctttaa taatgtgtag tttgttgggt taattgttgt
    18481 catatttaaa acctcttatt ttcatagatt ttataatagt atagaatttc gaaaatatta
    18541 tggcaataaa ttcattttat aatttatcct taagcaaaat tacgttataa tagtaatgat
    18601 aaattaggag gagaagcata tggtgttata tatcattttg gcaataattg tgattatatt
    18661 gattgctgta ggtgtattat tctatttacg ttcaaataaa cgacaaatta ttgaaaaagc
    18721 aatcgaacgt aaaaatgaaa ttgagacgtt accttttgat caaaaccttg cacaattatc
    18781 taagttgaat ttaaaaggtg aaacaaaaac gaaatacgat gcaatgaaaa aggacaacgt
    18841 agaaagtaca aataagtatc tagctcctgt ggaagaaaaa atccataatg ctgaggcttt
    18901 attagataaa tttagtttca acgcatctca aagtgaaatt gatgatgcaa atgagttgat
    18961 ggatagttac gaacaaagct atcagcaaca attagaagat gtaaatgaaa ttattgcgtt
    19021 atacaaagat aatgatgaat tatatgacaa atgtaaggtt gattatcgtg aaatgaaacg
    19081 tgatgtttta gcaaatcgtc atcaatttgg tgaggcagca agtctacttg aaactgaaat
    19141 tgaaaagttt gagccaaggt tagagcaata tgaagtacta aaagctgatg gtaattatgt
    19201 acaagcgcac aaccatatag ctgccttgaa tgaacaaatg aaacagctaa gatcttatat
    19261 ggaagaaata ccagaattaa ttagagaaac tcaaaaagaa ttacctggtc aattccaaga
    19321 tttaaaatat ggttgccgtg atcttaaagt tgaagggtat gatctggatc acgtaaaagt
    19381 agacagtaca ttacaaagct taaaaacaga gcttagtttc gttgaaccat taattagccg
    19441 cttagaatta gaagaagcta atgataaact agctaatatc aatgataagt tagatgacat
    19501 gtatgattta attgaacatg aagttaaagc taaaaatgat gtcgaagaaa caaaagatat
    19561 cattacggat aacttattca aagctaaaga catgaattat acattgcaaa cagaaattga
    19621 atatgtacgt gaaaactact atataaatga atctgatgct caaagtgttc gtcaatttga
    19681 aaatgaaatt caaagtttaa tttctgtata tgatgatatt ttaaaagaaa tgtctaaatc
    19741 tgctgtgcga tatagcgagg ttcaggataa tttacaatat ttagaagatc atgtcacagt
    19801 tattaatgac aaacaagaaa agctacaaaa tcatctgatt caattgcgtg aagatgaagc
    19861 agaagcagaa gacaatctgc tacgagtaca atcgaagaaa gaagaagtgt atcgtcgatt
    19921 acttgcttct aacttaacaa gcgttcctga aaggtttatc atcatgaaaa atgaaattga
    19981 tcatgaagtt cgtgatgtta acgaacaatt tagtgaacgt ccaatacacg ttaaacagtt
    20041 aaaagataaa gtgtctaaaa ttgtgattca aatgaataca tttgaagatg aagcaaatga
    20101 tgttcttgtt aatgctgttt atgcagagaa attaattcaa tatggaaata gatatcgtaa
    20161 ggactatagc aatgttgata agagcttaaa tgaagctgaa cgattattta aaaataatcg
    20221 ctataagcgt gcgattgaaa ttgcagagca agctcttgaa agtgttgagc caggtgttac
    20281 taaacatatt gaagaagaag ttattaagca atagaaacta gtatgtagtt atacttaaat
    20341 aatatgagca ctctgtcaaa ttggactgat gagtttaata attgaagtta gccaacgata
    20401 cgttgtctag cttctttttt atatggataa atgaaaggga caataaatat aaatagcaat
    20461 tgtttaaaga taaacgtaat caaatgtgtt gttttaatta atataagtag tgaaaaaagc
    20521 ataatcacac agctgtttaa atagagtgaa atagtctaat tcttatttaa taagtagaaa
    20581 taagattatt ttgcgctata acctttattt tttgtcggat aatagtataa tgcttgagta
    20641 aaacaatttt aacgattgat gattaatgaa agagaagtgt ttattttgat atatctagat
    20701 aatgcggcaa cgacgaaagc atttgaagaa gtgttagata cttatttaaa agtaaatcaa
    20761 tcaatgtatt ataatccgaa tagtccgcat aaagctggtt tgcaggcaaa tcaattacta
    20821 caacaagcaa aaacccaaat taatgcaatg attaattcaa aaacaaatta tgatgttgta
    20881 ttcactagtg gtgcaactga atccaataat cttgctttaa aaggtattgc ctatcgtaaa
    20941 tttgatacag cgaaggaaat aattacatcc gtgttagagc atccgtccgt attagaggtt
    21001 gtaagatatt tggaagcaca cgaaggattt aaagttaaat atgttgatgt aaagaaagat
    21061 ggcagtatta acttagaaca cttcaaagaa ttaatgtcag acaaagtcgg tttagtaaca
    21121 tgtatgtatg taaataatgt aactggacaa atacagccta ttccacaaat ggctaaagtt
    21181 ataaaaaatt atcctaaggc acattttcat gtagatgcgg ttcaagcatt cggcaaaatt
    21241 tcaatggatc tcaataacat agatagtatt agtttaagtg gacacaagtt taatggttta
    21301 aaaggacaag gcgtcttact tgtaaatcac attcaaaatg ttgaaccaac tgtccatggt
    21361 ggtggtcaag aatatggcgt tagaagtgga acagttaatt tgccaaatga tattgcaatg
    21421 gttaaagcga tgaagatagc taatgaaaac tttgaagcat tgaatgcatt tgttactgag
    21481 ttaaataatg acgtccgtca atttttaaat aaatatcatg gagtttatat taattcttca
    21541 acttcaggtt caccattcgt tttaaatatt agttttcctg gcgtaaaagg tgaagtatta
    21601 gttaatgctt tttcaaaata tgacattatg atatctacga caagtgcttg ttcatctaaa
    21661 cgtaataaat taaatgaagt attggctgca atgggattat cagacaaatc tattgaaggt
    21721 agtataagat tatcatttgg ggctactaca actaaagaag atatagcgag gtttaaagaa
    21781 atatttatca tcatttatga ggaaattaag gagttgctaa aataatgaag tatgatcact
    21841 tgcttgttag atacggggag ttaacattaa agggttcaaa tagaaagaaa tttgtaaatc
    21901 aattaagaaa taatgtaaat aagtcattaa aaggacttga tgggtttgtc gttaaaggca
    21961 aacgagatcg tatgtatatt gaacttgaag accatgcaga tataaatgaa ataacatatc
    22021 gattatcaaa aattttcggt attaaatcta ttagtccagt attaaaagta gaaaaaacaa
    22081 tagaggcaat aagtacagcg gcaattaaat ttgcgcagca atttgaagaa aacagcacat
    22141 ttaaaattga tgtgaagcgt gccgataaaa atttcccaat ggatacgtat gaattacagc
    22201 gtgaattggg tggtgcagta ttgaagcact tcgacaatat ttcagtgaat gtcaaacgtc
    22261 cagatcatga aattcgagtg gaagttagat tagatgcaat ttatatgtat gaagaagttg
    22321 ttccgggttc aggtggatta ccagttggta ctggtggtaa gacgttacta atgctttcag
    22381 gcggtataga ctcaccagtt gctgggatgg aagtgatgag acgtggcgta acaattgaag
    22441 cgattcattt ccatagtcca ccatttacaa gtgatcaagc aaaagaaaaa gttattgaat
    22501 tgacacgtat tttagctgaa cgtgttggac caattaaatt gcatattgta ccatttacag
    22561 aattgcaaaa acaggtaaat aaagttgtac atccaagata tacaatgact tcaacgagac
    22621 gtatgatgat gcgtgttgct gataaattag tacatcaaat aggggcttta gctattgtaa
    22681 atggtgaaaa cctagggcag gtagccagtc aaacacttca tagcatgtat gcaattaata
    22741 atgtaacttc tactcctgta ttacgtcctt tattaactta cgataaagaa gaaattatta
    22801 ttaaatcgaa agaaattggt acatttgaaa catctattca accatttgaa gattgttgta
    22861 caattttcac ccctaaaaat ccagtaaccg aaccaaactt tgataaggta gtccaatatg
    22921 aaagtgtctt tgattttgaa gagatgatta atcgtgctgt tgaaaatatt gaaacacttg
    22981 aaataactag tgattataaa actattaaag aacagcaaac aaaccaatta ataaacgact
    23041 ttttataaat aaaatcctag agtaaattta aacataaggg gatgttaaac tatggatttg
    23101 aacttaacga tgattataat cataatttta tttggtttta tcgcggcgtt tatagattcg
    23161 gttgtagggg gtggcggttt aatttctacg ccagcattat tagcaatcgg tctaccacca
    23221 tctgtagctt taggtacaaa taaattggca agttcatttg gttctttaac tagtacgata
    23281 aagtttataa ggtccggtaa agtggactta tttgttgttg ccaaattatt tggttttgta
    23341 tttttggcat ctgcatgtgg cgcatatatt gcaacgatgg ttccgtcaca aatattgaaa
    23401 cctttaatca tcattgcact ttcgtcggtg tttatattca cattacttaa aaaagattgg
    23461 ggcaatacac gtacgtttac tcaatttaca tttaagaaag ccataatatt tgcagcactt
    23521 tttatattaa tcggctttta tgatggattt gtaggtgggg gaacaggttc atttatgctt
    23581 tttgtattgt taatctttgg ttttgatttt ttaagtgcag caggaaatgc taaagttttg
    23641 aactttgctt ctaatatagg tgcgcttgta ttatttatgg tattaggaca agtagattat
    23701 gtaataggtt taattatggc tataagtatg attgctgggt catatgctgg ggctcatttt
    23761 gctattaaac aaggtgttgg ttatgtaaaa gtattattta ttattattac tgctatttta
    23821 attttaaaaa atgcttttga ttatattcaa caatttgtat aaaattaaat ttgcttttga
    23881 ttaaagcatg aaaattgtaa tcaaaccata aattgtcgta tgatgtagtt agaattttaa
    23941 aatgcaggag gtcaagtata tgactgaaat aacattcaaa ggtggaccaa tccacttaaa
    24001 aggtcaacaa attaatgaag gtgattttgc acctgatttt acagtgttag ataatgactt
    24061 aaatcaagta acattagcag attatgctgg taaaaagaaa ttaattagtg tggtaccatc
    24121 aattgataca ggtgtttgtg atcagcagac tcgcaaattc aactctgatg cttctaaaga
    24181 agaggggatt gtgcttacaa tttcagcaga cttaccattc gcacaaaaaa gatggtgcgc
    24241 ttcagcaggt ttagacaatg tcattacatt aagtgaccac cgtgacttat catttggtga
    24301 aaactatggc gttgttatgg aagaacttcg cttattagct cgtgcagtat ttgtattaga
    24361 tgcagataat aaagttgttt ataaagaaat cgttagtgaa ggtactgatt tcccagattt
    24421 tgatgctgct ttagctgcat acaaaaatat ttaatcatta aagagataaa tcttaaaatg
    24481 tatacatcgt gtccatcgtt gtcaacagca ttaaaataga attgttttct atgattgcta
    24541 agacctatgg gcacttttta ttggagaggg acgaatatgg cagaacaaca aacaattatg
    24601 gaacgcttgt ttcatacatt agatgaaaaa gctaaaacat taaataatga aaatggccaa
    24661 agttttattg aaaatcttgg gctagcaatg gaacaagtat ataccaatga aagaggattg
    24721 ttagaacaat cgacgttaca agatcgacgt aaagcattcc aatttgcata tttaagttta
    24781 atgcaggaag aaaagataca agcaaatcat caaattacac cagattcaat tggattgata
    24841 ctaggatttt tagttgagcg ttttatgaac aaccaagaag aattacatat tgttgatatt
    24901 gcaagtggtg ccggtcattt aagtgctact gtaaaagaag tgttacctga aattgcggtt
    24961 atgcatcatt taattgaagt tgatccagtt ttatcacgtg ttagtgtaca tttagcaaac
    25021 ttcttagaaa ttcctttcga tgtgtatcct caagatgcca tcatgccact accattagaa
    25081 gaagcagata tcgttattgg tgattttcca gtaggctatt atccaattga tgaaagaagt
    25141 aaggagttta agctaggttt tgaagaagga catagttatt cacattattt attaatagaa
    25201 caagcaataa atgtattaaa agatgctgga tatgcctttc tagtggtacc aagtaatatt
    25261 tttacaggtg aacatgtaaa acagcttgaa aaatatattg caacagagac agagatgcaa
    25321 gcatttttaa atttaccacc aactttattt aaaaatgaaa aagcgcgaaa atctatatta
    25381 attttacaaa agaaaaaatc gggtgaaaca aagccagttg aagtattatt ggcaaatatt
    25441 cctgatttca aaaatccttc acaatttcaa ggatttatga cagagttaaa tcagtggatg
    25501 gacacaaatc gtcctaaaaa ataatctgta atagtatagt cataaaactg tatgaatgat
    25561 aaaatgagaa atgaaatagt ataaaaagca aacgatggag gatcattact tatgtcaaaa
    25621 ttaatcttgg ctatcaatgc tggtagttca tcattaaaat ttcaattaat tagaatgcct
    25681 gaagaggaat tagtaacaaa aggtttaata gaaagaatcg gattgaagga ttcaattttt
    25741 acaattgaag tcaacgggga aaaagttaaa acagtacaag atatcaagga tcacgttgag
    25801 gcagttgata tcatgttgga tgcttttaaa gcgcacaata ttattaatga tattaacgat
    25861 atcgatggaa caggtcaccg tgttgtacat ggtggtgaaa aattccctga atcagtagct
    25921 attactgatg aggttgaaaa ggaaattgaa gaattaagcg aattagcacc gcttcacaac
    25981 ccagctaact tgatgggtat tcgtgctttc cgtaaattat taccaaatat ccctcatgtg
    26041 gcaatttttg atacagcatt ccatcaaaca atgcctgaaa aagcatatct atacagcttg
    26101 ccatatcatt attataaaga ttatggcatt cgtaagtatg gtttccatgg tacaagccat
    26161 aaatttgtat cacaaagagc ggcagaaatg ttagataagc caattgaaga tttacgtatt
    26221 atttcatgtc acattggtaa tggagcatca attgcagcta tcgatggcgg taaatctatt
    26281 gatacatcaa tgggcttcac accacttgct ggtgtaacaa tgggtactcg ttctggtaat
    26341 attgaccctg ctttaattcc atttattatg gaaaaaacag gtaaaactgc tgaacaagta
    26401 ttggaaatct taaacaaaga atcaggttta ttaggattat caggtacatc tagtgattta
    26461 cgtgacttat cagaagaagc tgaatcaggt aaagcacgtt cacaaatggc tttagatgta
    26521 tttgcttcta aaattcacaa atatattgga tcatatgctg cgagaatgca tggtgttgat
    26581 gttatcgtat ttacagcagg tatcggtgaa aactcagtag aaattcgtgc gaaagttctt
    26641 gaaggcttag aatttatggg tgtttactgg gatcctaaga aaaatgaaaa cttactacgt
    26701 ggtaaagaag gatttattaa ctatcctcat tcaccagtta aagttgtcgt tattccaaca
    26761 gatgaagaaa gtatgattgc ccgtgatgtt atgacattcg gtggtttaaa ataattttta
    26821 aataaatatt aagcgtctat ctccgctaca attatatgta ggaggtagac gctttttgta
    26881 ttgtttgaaa attaactagt gtaatgtctc ttttcacgaa tatcttaaag aattaagtag
    26941 tcgaagaaga ttatgaaaac attattaaat gagttttatt tataaacaat aaaaaaccaa
    27001 gtgcagaaag ttgcacttgg ttcataatga tcaagtagtt atctaatgat catctatatt
    27061 taattttgat atttttcacg taattgagtt gttgcaactt gtggttggaa gtctgctggt
    27121 aactcttcag tacgaacaac taacacgtca catggcgcat gacgaacgat agattctgat
    27181 acagaaccaa caataaatct ttccacggca tttaagcctg atgtaccact cataatcaag
    27241 tctgcattaa tttcatgtgc aagcttttta ggaatgatag atttaggaga gccaaactct
    27301 agacgcgttt ctacatcttt aacaccagcg ttagtagcta cttctttata accatttaat
    27361 aattcttctg caaaatgctt agatttttca gtaaattgag catcataaac ttcataagaa
    27421 gaatacgttc ttgaatcaat tacattcaca attgttaact tcgcatcgtt acgtttagca
    27481 acaccaactg ctctgttaaa tgcccattcc gcttcatgtg aaccgtcaac tgcgattaaa
    27541 atatttttgt aagtaatcat agtaagtgcc tccttgttct ttcttaatta tattttacca
    27601 caattttcgg aaaagtcagt gtaataacct ttacaatttt gtggattttt gattgcgctt
    27661 tcaaaatgtt cgcgttaaga tgattatgga ggtggatggt atgaaaattg gtataccaag
    27721 ggagattaaa aataatgaaa atcgtgttgg tttatcacca agtggtgtgc acgctttagt
    27781 agaaagtggg catactgttt tagtggaaac aaatgcgggt tcaggatcgt tctttgaaga
    27841 tgtagattac aaagaagcag gtgctgagat tgttgctgaa caagcaaaag tttgggatgt
    27901 ggatatggtt attaaagtta aagaaccact tgaatctgaa tatccatatt ttaaagaagg
    27961 gcttgtatta ttcacttatc ttcatttagc aaatgaagaa aaattaacac aagctttgat
    28021 agatagaaaa gtaattagta ttgcatatga gactgtgcag ttaccagacc gatctttacc
    28081 attgttatca ccaatgagtg aggtagcagg aagaatgtca gctcaagttg gtgcagagtt
    28141 cctacaaaaa ctcaatggtg gtatgggaat tctactaggt ggtgttccag gagtacctaa
    28201 aggtaaagta actattatcg gtggtggtca agcaggaaca aatgcagcta aaattgcact
    28261 aggactaggt gcagatgtta cgattttaga tgttaatcca aagcgtttac aacaattaga
    28321 tgatttattc ggtggacgtg tacatacaat tatgtcaaat ccgttgaata ttgagttgta
    28381 tgttaaacaa agtgatttag taataggtgc agttttaatt ccaggtgcta aagcgccaag
    28441 acttgtaaca gaagacatga ttaaacaaat gaaaaatggg tcagttatta ttgacattgc
    28501 tattgatcaa ggcggtattt ttgaaacaac tgataaaatt acgacacatg atgatcctac
    28561 atatattaag catggtgtgg ttcattatgc agttgcaaat atgccaggtg cagtaccgcg
    28621 tacttcgacg ttagctttaa ataatgctac gctaccttat gcgctcatgc tagctaataa
    28681 agggtataga gaagcattta aatcaaatca accattatca ttaggtttaa atacttacaa
    28741 aggtcatgta accaataaag gcgttgcaga ggcatttgaa atggaatata aatctgtaga
    28801 agaagcatta caattataat ttgaatttaa tgaaaaggga atggattaca gcgtactaaa
    28861 gaatgacgtt atcatctcct tggttattaa aattaaataa tgataaatta aagctagaaa
    28921 caatgcgaag aaatcatcga acatgaatga tattgatttg attcattaca tgttactgat
    28981 gaacatacct gcatatgtgt ttctagcttg tttgttacat attgcattta gacttggata
    29041 aaaatttatg tcttaggcgc tacaatgtta tattggcagt gtttgactga atgagaatgc
    29101 gcttgtaaca agcttttcat ttttatccca ctccttattt ttcgtaatgt gttaatactt
    29161 catatccttc attagtgaca agtatgtcat cttcaattct tacacctgca acacctggta
    29221 cataaatacc tggttcgatt gtaataacca tgccagcttc taacaaatta gaattagtac
    29281 ttgaaacatc ttgatattca tgttcttgta atcctaaacc atgacctaag cgatgaggga
    29341 aatattcacc ataacctttt tctaaaataa tatttctagc gatatgatcg atatctttta
    29401 aaggtattcc aggtttaatt gcttggattg cagatgtttc tgcttctaat acaatattat
    29461 aaatttcttg tgcttctttg ctaggttcac caaatttaat ggtacgtgtc atatcgctac
    29521 aataatgctc ataaattaca cctaaatcaa atagtacata ttcattgctt tttaatctgc
    29581 gatctcctgg tgtgccatga ggtgatgcgg catgatctcc aaataaaacc atcgtatcaa
    29641 aactcatttc attgacgcca tattgtttga tagtttgctc aatatggttg actacttcac
    29701 gttcagtcac accttctttt aaataagaaa cacctatttc gatacactta tctgctaact
    29761 cagcagcttt acgtattttg ctaatttcat cttcggattt aatatttctc aattgtttga
    29821 ttgttaaatc aacatctccg aatgaattga cattgaaacc agaggttaat tgtttttggc
    29881 gtgctactgt taagtgctcg ctttcaatta gtaatttatt gattgtttga ggataaagtg
    29941 aaaaagggtt ttcagtgtct aaatatccaa cgatttcacc tgtgaaaggt gatgctttga
    30001 cttcttcgac ttccattttt ggacaaaata gtacttgttt accatctttc ttaatcaata
    30061 atgcaaataa tctttcatgg ggttcgctac ggtatccagt aaaataatat acattcaacg
    30121 gtgttgtaat ccatgctgca tcagcttgtt gattgttcag ttcgtctatt atttttgata
    30181 tttttgtcat tttataaaca ctcctcaaca taatattact tccattttat gccaattatt
    30241 attagatttc aaaatcgagc taatacatta actagatttt caatttttaa aatttgagaa
    30301 tttgttataa ttaagtggca aaagggtact aaatttatat agcaattgat aggaggatgc
    30361 tcaaatgaag ttatcatttc atggtcaatc aacgatttat ttagaaggga acaacaaaaa
    30421 ggttatcgta gatccgttta tttcaaataa ccctaagtgt gatttgaata ttgaaacagt
    30481 gcaggtagac tatatcgttt taacgcacgg tcattttgat cattttggtg atgttgtgga
    30541 actagcaaag aaaacaggag caacagtaat tggaagtgca gaaatggctg actatctttc
    30601 ttcatatcac ggtgttgaaa atgttcatgg tatgaatatt ggtggtaaag taaactttga
    30661 tttcggaagc gttaaatttg ttcaagcatt tcatagttca agttttacac atgaaaatgg
    30721 tattccagtt tatttaggta tgccaatggg tatcgtgttt gaagttgaag gaaaaacaat
    30781 ttatcatact ggtgatacag gtctgtttag tgacatgtca cttattgcga aacgtcatcc
    30841 agttgatgta tgttttgtac caattggaga taattttacg atgggtattg atgatgcaag
    30901 ttatgctatt aatgaattta ttaaaccaaa aatttctgta ccgattcatt atgatacatt
    30961 cccattaatc gaacaagatc cacaacaatt taaagatgct gttaatgtag gggacgtgca
    31021 aattttaaaa ccaggtgaat ctgttcagtt ttaaatagaa tatagatttt acactaaaaa
    31081 acacctcgct aatctatgtg gaagttatcg tctgcatatt tttagtgggt gtttttatat
    31141 tctactgatt cagttacgac gtggacgggg gacatcagtg gttctttatc atttctgacc
    31201 tactctcgta attttagttt tatacaatta gacattgatt ataaatttac tacgatataa
    31261 aaaatccaga accacagtca taatcatttc ttgtgtttaa tcagtcgttt gagactgcat
    31321 catgattgaa agattatttg tgattctgga taaaaatttt atttaacgat taatacaggg
    31381 atagtcgcac gttttgctac tttgtggctg acacttccaa gtacaaattt ctttttgtct
    31441 tctgctttac ggttacttaa aacaacaatt tcgtatttac cgctattagc atgttttaat
    31501 agttcttctt ttgcattacc acgaacaatt atttggtcat aatcaatacc ataatcttct
    31561 aaagctttgc gtgttttttc caatcgcttg cttcgttctt cagtaagttt gtttaaatga
    31621 acacctgctt taattgatgc ttgagcatct tgttcgctga ttgcgtttaa aactgttacg
    31681 actgtacctt cgccagctaa tttagacact tcttttagtg ctttttcatt ttttaactga
    31741 gtgtctacac caagtaatat atttttatac attagtacat ccctcctcac ttacattcta
    31801 ataaataata atggattttt ccatttaaaa tt
//
LOCUS       DAHHYJ010000015        28178 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000015 DAHHYJ010000000
VERSION     DAHHYJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.015"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..613
                     /locus_tag="NBK22_000521"
     CDS             <1..613
                     /locus_tag="NBK22_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413729.1"
                     /translation="SVYLHFFKNGINDKDIDSSNRIKENLYYFINHEITDQRDYEHAL
                     TNYYLYFLVDSNIEIGIKSIIIKIFTEKGITSKDKLQILEYYLDEIDDLNTEISTKIA
                     DAHVKNSTNFLENEKVSINSNLAKYITHRDTRASAPKNMLLSLILNSDLVSPKDLEEK
                     ELKYPKKITKEHHDKLIKEINKALDYYNDNNTTPSDYFYITSD"
     gene            806..1102
                     /locus_tag="NBK22_000522"
     CDS             806..1102
                     /locus_tag="NBK22_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD2413730.1"
                     /translation="MYFNQSDKFEQLKKKILGISFNLPEGEEFTFHDLNSRANVQCSK
                     AVQQNVGRWFAYFVKQAPRVPFIIIGKDTHGHLVYLKTGPNPHYNSNTSKGGDC"
     gene            1102..2889
                     /locus_tag="NBK22_000523"
     CDS             1102..2889
                     /locus_tag="NBK22_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCD2413731.1"
                     /translation="MSYTLFEIGPFALDSFGWKETKPPKKDGDREKVVIMASPIIVNA
                     RFSDPVTGVEKLIITNNNGKKDIFESDILTTRNLPSLIKYGYSINEKHIRSLSYALQL
                     MRDRLPLSELYEGVGILETPFGYLISLDKVLNSVQFNQSSPSYPIVDSAYDLTPKGTF
                     DNWFNMYIDEVKGHLLLELAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKST
                     AAALAVSVAGNPTKGNETLFRSWNATRNALEGYLSNNYGVPIVFDELSSTTLRDTTGL
                     LYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQF
                     TTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDETNNT
                     GIRMFKRYAVITTSAKILGRVLSTDIDIANIRDYFIDYHTHTVSERSLADKAIDVIIQ
                     FVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNVVN
                     ALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDEKFASILGLTKDKSLLQNW
                     TPSNDNKAAKEFFKSANDGIGPSGVHEDF"
     gene            3123..4472
                     /locus_tag="NBK22_000524"
     CDS             3123..4472
                     /locus_tag="NBK22_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2413732.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGHKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
                     NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KGAIDKRIKRQRNIEKHHILTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHTIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQAMQSSIA"
     gene            4494..6122
                     /locus_tag="NBK22_000525"
     CDS             4494..6122
                     /locus_tag="NBK22_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2413733.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            6644..6994
                     /locus_tag="NBK22_000526"
     CDS             6644..6994
                     /locus_tag="NBK22_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413734.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            7081..7392
                     /locus_tag="NBK22_000527"
     CDS             7081..7392
                     /locus_tag="NBK22_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCD2413735.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            7407..7913
                     /locus_tag="NBK22_000528"
     CDS             7407..7913
                     /locus_tag="NBK22_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCD2413736.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIRTPENLKHIKNPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            7965..9572
                     /locus_tag="NBK22_000529"
                     /pseudo
     CDS             7965..9572
                     /locus_tag="NBK22_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(9563..9823)
                     /locus_tag="NBK22_000530"
     CDS             complement(9563..9823)
                     /locus_tag="NBK22_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCD2413737.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(9795..10781)
                     /locus_tag="NBK22_000531"
     CDS             complement(9795..10781)
                     /locus_tag="NBK22_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCD2413738.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            10878..12887
                     /gene="mecA"
                     /locus_tag="NBK22_000532"
     CDS             10878..12887
                     /gene="mecA"
                     /locus_tag="NBK22_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCD2413739.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(12933..13361)
                     /locus_tag="NBK22_000533"
     CDS             complement(12933..13361)
                     /locus_tag="NBK22_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCD2413740.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(13458..14201)
                     /locus_tag="NBK22_000534"
     CDS             complement(13458..14201)
                     /locus_tag="NBK22_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCD2413741.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(14958..15329)
                     /locus_tag="NBK22_000535"
                     /pseudo
     CDS             complement(14958..15329)
                     /locus_tag="NBK22_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            15383..16057
                     /locus_tag="NBK22_000536"
     CDS             15383..16057
                     /locus_tag="NBK22_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCD2413742.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSK
                     YLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEI
                     SIMLAS"
     gene            complement(16089..16328)
                     /locus_tag="NBK22_000537"
     CDS             complement(16089..16328)
                     /locus_tag="NBK22_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413743.1"
                     /translation="MSDNLSLFIDYINDNIIYGSEIKREKLENLFNQFAIKNVEKNIV
                     YDELKSLDITIIESQDSYKNKLKRLFSVLLQSKKI"
     gene            complement(16743..17999)
                     /locus_tag="NBK22_000538"
     CDS             complement(16743..17999)
                     /locus_tag="NBK22_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCD2413744.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKDVDDLIDKVFNVEAIQYKVFGEK"
     gene            complement(18321..18800)
                     /gene="rlmH"
                     /locus_tag="NBK22_000539"
     CDS             complement(18321..18800)
                     /gene="rlmH"
                     /locus_tag="NBK22_000539"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCD2413745.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(19168..21486)
                     /gene="adsA"
                     /locus_tag="NBK22_000540"
     CDS             complement(19168..21486)
                     /gene="adsA"
                     /locus_tag="NBK22_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCD2413746.1"
                     /translation="MKALLLKTSVWLVLLFSAMGLWQVSNAAEQHTPMKAHAVTTIDK
                     ATTDKQQVPPTKEAAHQSGEEAATNVSASAQGTADDTNNKVTSNAPSNKPSTAVSTTV
                     NETRDVDTQQASTQKPTHTATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLMLDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKITFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAIADAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDINKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGTREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSESGKDAQPIGDDKVMDPA
                     KKPAPGKVVLLLAHRGTVSSGTEGSGRTIEGATVSSKSGKQLARMSVPKGSAHEKQLP
                     KTGTNQSSSPEAMFVLLAGIGLIATVRRRKAS"
     gene            complement(21714..22514)
                     /locus_tag="NBK22_000541"
     CDS             complement(21714..22514)
                     /locus_tag="NBK22_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD2413747.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(22904..23692)
                     /locus_tag="NBK22_000542"
     CDS             complement(22904..23692)
                     /locus_tag="NBK22_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCD2413748.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIKVPTSILNKSVKGIKLEQITGRSKDFSSKAKGDSDLTTSDGGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFNI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVIGVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(23693..25027)
                     /locus_tag="NBK22_000543"
     CDS             complement(23693..25027)
                     /locus_tag="NBK22_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413749.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFILFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKSLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(25020..26846)
                     /gene="walK"
                     /locus_tag="NBK22_000544"
     CDS             complement(25020..26846)
                     /gene="walK"
                     /locus_tag="NBK22_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCD2413750.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKTNDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(26859..27560)
                     /locus_tag="NBK22_000545"
     CDS             complement(26859..27560)
                     /locus_tag="NBK22_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2413751.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
BASE COUNT         9409 a         5013 c         4265 g         9491 t
ORIGIN      
        1 ctcagtttat ctacactttt ttaaaaacgg tatcaatgac aaagatatag attcttctaa
       61 tcgtatcaaa gagaacttat attatttcat caatcatgaa attactgatc aaagagatta
      121 tgaacatgct ctgactaatt attatctcta ttttctagta gacagtaata tagaaattgg
      181 aatcaaatct atcattatta agattttcac tgaaaaaggt atcacttcta aagataaact
      241 tcagattctt gaatattatt tagatgaaat agacgattta aacactgaaa taagtaccaa
      301 aattgctgat gctcatgtga aaaatagtac aaacttttta gaaaatgaaa aagtaagtat
      361 aaacagtaat ttagcaaaat atattactca tcgagacacc agagcttcgg cacctaaaaa
      421 tatgctgtta tcattaattt taaatagtga cctagtgtct ccaaaagact tagaagaaaa
      481 agaactcaaa tatccaaaga aaataacaaa agaacatcat gataaattaa taaaagaaat
      541 caataaagca ctagattatt ataatgataa taacaccact ccttctgact acttttatat
      601 aacttcagat taatttttat ttaaagcgct gttaaatcag cgttttttat tttgtcaaca
      661 aaaatccatc ttaatttagt acggaatttc taacatattt ttacaagctt tcaaatgata
      721 acttcttggc taaaatgaac ttaagaaagt taattgtgcg catatttaat tttcctatct
      781 tagttcatta gtaggaggaa gttaaatgta tttcaatcaa tcagataaat tcgagcagtt
      841 aaaaaagaaa atattaggta tttcattcaa tttacctgaa ggagaagaat ttacatttca
      901 tgacttgaac agtcgtgcaa atgtacaatg ttcaaaagct gtacaacaaa atgttggacg
      961 ttggttcgca tattttgtaa aacaggcgcc acgtgttcca tttattatta tcggtaagga
     1021 tactcacggt cacttagttt atcttaaaac aggtcctaat ccacactata acagcaacac
     1081 ttcgaaagga ggtgattgct aatgagttac acattgtttg aaattggccc atttgcttta
     1141 gattccttcg gttggaaaga aacgaaaccg cctaaaaaag atggtgacag agaaaaagtg
     1201 gttataatgg ctagccctat tattgttaat gcaagatttt ctgatccagt tactggcgtt
     1261 gaaaagttga ttattactaa caacaatggt aaaaaagaca tttttgagtc tgatatctta
     1321 acaacacgaa acttaccttc tttaattaaa tatggataca gtattaacga gaagcatatt
     1381 agatcattga gttatgcctt gcaattgatg cgagatagat taccactttc tgaattatat
     1441 gagggtgtag gtatactaga gacacctttt ggctacttga tatcgctaga taaagttctc
     1501 aactctgttc aatttaatca gtcctcacct tcatacccta ttgtagatag tgcttatgac
     1561 ttaactccta aaggtacatt tgataattgg tttaacatgt atattgacga ggttaaaggt
     1621 catcttcttt tagaattggc agtcatattt gggatttctg ccttagtgac aagttttctg
     1681 aagcataaac atgaaatcga atttgcagga attctcttta gttttacagg tcaatcctca
     1741 accggtaagt caaccgcagc tgctttagct gtatcagtcg ctggtaaccc taccaaaggt
     1801 aatgaaactc tttttagaag ttggaatgcc acacgtaacg cacttgaagg ttatttgagt
     1861 aacaattatg gcgttcctat agtatttgat gagctttcat ctacgacact tagagatacc
     1921 acgggtttgt tatattccat cgctgaaggt caaggtcgtc aacgttcaaa cgttcatgga
     1981 gaagttaaaa cgcctaagaa ttggggtact tctgtcattt caacgtcaga atacagtatt
     2041 tttaatgatt cagctcaaaa cgatggatta cgtgtaagaa ccatcgagat taatgaacaa
     2101 ttcactacaa atgcgaccaa tgctgacaat attaaaaaag ctgtagcact aaactatggt
     2161 catgttttac cgttagtagc taaatatctt ataaatcgtg aagatgaagt tattcaatgg
     2221 ttttacaagg aagtagattg gtttgaagct aaattgaaag atgaaacaaa caatacaggt
     2281 attcgtatgt ttaagcgtta tgcagtcatt acaacatctg cgaaaatatt aggtcgtgtt
     2341 ttatctacag atattgatat cgctaatata agagactact ttattgatta tcatactcat
     2401 acagtctctg aacgctcatt agctgataaa gctattgatg tcatcattca atttgtggca
     2461 caaaacagag gtaagttttc agatgaaggt gctttgaaaa atatgtttga gaactatgga
     2521 ttaatctcat taaaagatga ccatatcgaa gtaaaaataa ttgcgaacgt ttttaaacag
     2581 atgctaaaca atcatcaatt tcaagatgtt aataatgttg tcaacgcctt aagagataaa
     2641 ggtttcatat tagctgatcg tggacgccaa acaactaaaa gaagtgtcaa agataatagc
     2701 ggtaaaaaac aatcacttgt attttaccac ctaaaattag atgaaaaatt tgcatctatc
     2761 ctcggattaa ctaaagataa gtcgttactt caaaattgga caccgtcaaa tgataacaaa
     2821 gctgcgaaag aattttttaa atccgctaat gatgggatag gtccttcagg agttcatgaa
     2881 gatttctaat tgtctttatc atacagctgt gaagcatcaa actgaataat cgacgtgttg
     2941 atgattgaac aaataacgga gtgaagaaca tggatgaact tatcgaggta ttagccgatt
     3001 tggtaattga agaaatcaat caaatgaatg actaatatga acgcccaaca tcttattgat
     3061 gttgggctta tatatcaatt catccataaa tatatattat tactgtgaaa ggaggagccg
     3121 atatgaaaca agtcataggc tatttacgtc aaagtacgat gaaacaacaa tctcttgcag
     3181 cacagaaaca ggctatcgaa gcaatagccg aaaaacatca tattcaacat atcaactttt
     3241 atagcgacaa acaatcagga cacaaagata atcgtagtgg gtatcgacaa atgacgcaat
     3301 taattcaaca agggcaatgt gacatattat gttgttatcg tcttaataga ttacatcgta
     3361 atctgaaaaa tgcattaaaa ctcatcaaat tatgtcaaac ataccatgtt catatcttaa
     3421 gcgtacacga tggttacttt gatatggatc aagctttcga ccgactcaag cttaatatct
     3481 tcatcagttt agccgaactt gaatcggata acattggaga acaagtcaga aatgggcttc
     3541 aagaaaaagc aaagcaaggt agattgatta caacccatgc gccctttggt tacgaatatc
     3601 acaacggaac attcatcatc aatcaaaatg agtcaccaac ggtaaaggct gtattcaatt
     3661 attacattaa aggtcatggt tataagaaaa ttgcacagtt attagaagaa gataacacgt
     3721 atatcaatcg acaaccctat caagttcgta acattattat caatcctaat tattgtggtc
     3781 gtgtcaacaa tcaatatggt caattcgaca atatgtttcc ttctattgtt tccacaagta
     3841 tatatgagca agcgcagaga cttcgattgc aaaaacaaac caaacagaca ccttcggata
     3901 atcaactcaa acaaaaaatc aaatgcccat gttgtaatgc aacacttaca aatatgacca
     3961 ttagaaaaaa gaatcataca ttacgttact acgtctgtcc taaaaacatg aatgcttcac
     4021 gctttgtctg tgattttaaa ggcatcaatg cacaaacact tgaagataaa gtattagaag
     4081 tgtgccgaga cttttatcaa aatcaacgca tctacacaaa aattaaaggt gcgattgaca
     4141 aacgcatcaa aagacaaaga aacatagaaa aacatcacat attgactcaa gaacaactga
     4201 tagaaaagtt ggcacaaggc atcattgatg cagaaacgtt cagagaacaa acgcaatcat
     4261 tacgtcaaca accgcaacgc actacatcta taaatgggca tcaaatacaa cacaccattc
     4321 aaaatattat tcaaaaacgt ttcacgttaa acatattgta cccctatatt gaaaccattc
     4381 acattacgaa agataaaaat cttataggaa tctatttcaa aaatgaacca ctcaatatcg
     4441 tcaatcaagc catgcaatca tcgattgcat aaatgaagaa aggatgtaac ataatgcaac
     4501 aacttaaaac aaaacgtgtc ggtatctatg tacgtgtatc aacagaaatg caaagcacag
     4561 aaggttatag tatcgacgga caaatcaatc aaatcaaaga atactgtgac ttccatcatt
     4621 ttgaagttaa agatatatac gctgaccgtg gtatttcagg taaatctatg aacagacctg
     4681 agctccaacg tatgttgaaa gatgcaaaag aaggcaatat cgactgtgtt atggtataca
     4741 aaacaaaccg attagctcgt aatacatctg atcttctcaa aattgtcgaa gatttacaca
     4801 aacaaaatgt cgaatttttc agtttgtcag agcgtatgga agtcaatact tcttctggta
     4861 aactcatgtt acagatactt gcgagtttct cagaattcga acgtaataac attgtcgaga
     4921 acgtatttat gggtcaaacg agacgtgccc aagaaggcta ttatcaaggc aatttaccac
     4981 taggttatga caaaatacca gatagtaaac acgagctaat gattaaccaa catgaagcta
     5041 atattgtaaa atatatattc gagtgctatg ccaaaggaca tggctatcgt aaaattgcca
     5101 atgcattgaa tcacaaagga tatgtcacta aaaaggggaa acctttcagt attagttcaa
     5161 tcacatacat cttagctaac cctttctata tcggcaaaat tcaatttgcg aaatacaaag
     5221 attggagtga aaaacgtcgt aaagggctga atgataaacc agtgatagct gaaggtaagc
     5281 attcccccat tattaatcaa gatttatggg ataaagtaca aatgcgtaag aaacaagtca
     5341 gtcaaaaacc ccaagtccat ggcaaaggaa cgaatctgct tacaggcatt attcactgtc
     5401 cccaatgtgg cgcacctatg gcagcaagca ataccacgaa tactcttaaa gacgggacta
     5461 agaaacgtat tcgttactat tcatgtagta attttcggaa caagggttcc aaagtatgtt
     5521 cggcaaacag tgtaagagct gatgtgattg aagattatgt gatgaagcaa atacttgaaa
     5581 tagtcaaaag tgataaagtc attcaacgcg tagtaacaca cgtcaatcaa gaaaatcaag
     5641 ttgatggcgc tgcacttcat cacgatattg cttataagca acaacaatat gatgaagtac
     5701 aaatcaaaat aaataacttg attaaaacca tcgaggataa tccggactta acatcagtaa
     5761 tcagaccaag tattcaaaaa tatgaaaagc aactcaatga cattacgaat caaatcaacc
     5821 aactcaaaaa tcaacaaaat gaagataaga ctttatttga tgccaaagaa atcagtaaac
     5881 tattacaaca catctttcat gatattaagc acatagaaaa atctcgactc aaagcattgt
     5941 atctatcagt gattgatcgc attgatatta aaaaagatgg taatcataaa aaacaattct
     6001 atgtcacact caaacttaat aacgaaatca ttaaacaact tttcaataat aaacaactcg
     6061 acgaagtgca tctcagcact tcgtctttat ttttgcccca aacactatat cttactattt
     6121 agctaataga atcattgtta taacaataat tttcactata ttctttcgct tattttatct
     6181 aactattttc aagaattgct ctttatcaat ttcaataaca cttatctaat gaaacagtta
     6241 ttgtcgtaat tttttcataa ctatacaatg cttatattga aattgctaaa gaacatccca
     6301 caatgatttt ttatcgggaa atgattggga tttgcagatt cgaagaaaca gaaggattgt
     6361 gcaagcacaa tcccaaaatc ccgttacttt ttataaataa ataatatata taaggaagca
     6421 gagacacaac cctatcatca atacttataa ataatgagtt gattgtttgt caaaataggc
     6481 ggaatttcag gatttccttg gcataccaac gtttgtagcg ggtttttata tggaattttc
     6541 tcttattccc accgggattc gtaataaatg acaaatcaaa tatatattat ctcaatgatg
     6601 tatatgaact acatcactat tacactatct aggaggcaat aatatgaaaa ccatcactca
     6661 agaactcaaa caatatataa cccacctatt ccaactatct aataacgaag cttgggaatg
     6721 tgaagcgctg gaggaagcag cagaaaatat tctaccggaa cgttttatta atgattctcc
     6781 actggtacat ctcacactcg agacttatac ctactataat gatgaactac atgagctaag
     6841 tatctatcca tttctaatgt atgccaataa ccaactcatc agtgtcggat acttagacca
     6901 ttttgacatg gactttctgt accttacaga tactcaaaat atcattattg atgaacgtca
     6961 tttactaaaa caaggaggtc aggaccatga ataattggat catagttgct caattatccg
     7021 tcacagttat caacgaaatc atcgacatca tgaaagaaaa gcagaaggga gggaaatagc
     7081 atgaaaatca atcgatacat cacaagagga attaatgaaa gtattccact agaccttcaa
     7141 atcttacttt ggcacatggt agaaaaaaaa gataaccagc ctcatacaga ttacctacac
     7201 atttttaaat tacaagaaga tgagaatata ctctcaatca cacatgaaca agaacaaccc
     7261 acatacaaat tggaatatca ctatacaaac tatgaaaaaa atcaaaatgc attacctaag
     7321 aaagtctacg tcattcgtga agatgacgtg gacgcttttt attatgtgat gcttttacca
     7381 gaagaatact aaaggaagtg acgatgatga atacaatcaa aagtacgata cacacagaag
     7441 ccatatttag cgatgatgaa cagcaccgct atttactcaa gaaaacatgg gatgaaaaga
     7501 aagctgcatg cacagtgata acgatgtatc ctcatttaga cggtgtatta tcactcgatc
     7561 tcacaactgt tcttatcctc aaccaattag ccaattcaga acgatacggt gctgtatatc
     7621 ttgtaaatct attctctaat attagaacac ccgaaaacct caaacatatc aaaaatccat
     7681 acgatgagca cactgatatt catttgatga aagcgattag tgaaagtgac acagtgattc
     7741 ttgcttatgg tgcctatgcg aagcgaccag ttgttatcga ccgtgtcgaa caagtgatgg
     7801 aaatgttaaa acctcataaa aagaaagtaa aaaagctcat caatccagta acaaatgaaa
     7861 ttatgcatcc actcaaccct aaggcacgtc aaaaatggat tttgaaatca tagcaaatac
     7921 atggaggtca atatggaaac aaaacaaatc aacatcgttt tactgtaaat gatattgttg
     7981 aaacaattcc tgacaatgaa ttcgacgaat tcagacatca ccgtggtgca acttcgtacc
     8041 atcctaaaat gatgttaaaa gtgattctat atgcctacac acaatctgta ttctcaggtc
     8101 gtaaaataga aaaaatgctt aatgatagca tccgaatgat gtggctatca caaaatcaaa
     8161 aaccttctta taaaacaatt aatcaattta gagtaaatcc aaaagtagat gctttattag
     8221 aatctttatt tattcaattt tacagtcagt gtgtaaaaca aaatcttata gatgataaag
     8281 ctatttttat tgatggtaca aaaattgaag caaatgccaa tcgatataca tttgtatgga
     8341 aaaagagtat tcaaaaccat gaatcaaaga tgaatgagga ttctaaagcc ctctaccatg
     8401 aattggtaac caataaaatc ataccggaaa ttaaagaaga tcatgataat gaattaacaa
     8461 aagaagaaat agatttgatt ggtagtcact tagataaaga aatcgaagat ttaaaccaac
     8521 atatcaacaa tgaaaaatgt actaaaacaa gaaaacaaat acgtctcaaa agaactaaaa
     8581 tcaaaaaata caaaaagcaa atcaatgatt attttgagcg aaagtatcga tacgaatttc
     8641 aaaaatctat tttaaaggat agaaatagtt attctaagac agattatgat gcgacattta
     8701 tgagaatgaa agaagatcac atgaaaaatg gacaacttaa gccagggtat aatttacaaa
     8761 tagcaacaaa ttcccaattt gttttatctt ataatgtgta tcaaaatcca acggatacta
     8821 gaacgatgat tccattttta aattcaattc aagagaccta cggtcattta cctgaatata
     8881 ttgtagctga tgcaggttat ggtagtgaat caaattataa ggcaattata gatgacttta
     8941 atcgaacgcc actcataaca tatggaatgt ttataaaaga taaaactaaa aaatataaaa
     9001 gtgacatctt taatactcaa aattggaact atgacgaaat taatgacgaa ttcatttgtc
     9061 cgaataataa acggctaggt tttaaaagat atgcctatcg tcatgataag tatggttata
     9121 agcgagactt caaattatat gaatgtgatg attgttcaga atgtcctctg aaaaatcaat
     9181 gtatgaactt caattcaaaa acaaacaaaa aaataatgaa gaattataac tgggaatatt
     9241 ttaaatccca aattaacaaa aagctttcag aaccagaaac aaaaaatatc tacagtcaaa
     9301 gaaaaattga tgtggaacct gtttttggat ttatgaaggc tattttgggt ttcactcgga
     9361 tgtctgtccg aggactcaat aaagtcaaaa gagaacttgg ttttgtatta atggcactta
     9421 atataagaaa agtagtagct caacgagctg aaaataatca aaaaatttat aaaaaagaca
     9481 atttctatat tatttcaata gaaattgtct tttttcactt atccaagaac tttatgtccc
     9541 ggactcatta tgatttattt ttttattgct gacttgccat taattctgct tgctctacaa
     9601 cttgttccac agccattttt tgaagatctg gtggatagcc atatttcttt aataggcgtc
     9661 taacttgaac tctcatttta gctttagcac tgtctcgttt agaccaatca acactcatgt
     9721 tatccttaac tgttttagtt aactcatgtg caatggctct taactcttta tcgcccaaag
     9781 cttctttagc tgtttcatgt gaagctcgat atactacata caatatacgc aacaatacgc
     9841 ttgtttcgat taggtttagg cattgaatca taaagtgcaa tatacttaac acgttcttta
     9901 atatttgaat taaaacctag taaatattgt gctgccacat tatttatgtg ctgagatttt
     9961 aaaatagagc attttaatat cgattcacca taacgtatat gttcatggcg attcaaaatt
    10021 tttaaaacgt ttctatcaca tactttttca cagtcattgt ccatcattgt tttacttata
    10081 tatagtgcag gattaaacca gaatatcatt ttaaaaacaa cataaagctg gttgaatatt
    10141 aagtcatgac ttttcacatg tgatagttca tgtagaataa tatattcaat ttctttgtca
    10201 ttcatggttt cgactacgac agttggtagt acaatttggg atttcactaa accaaatacc
    10261 atcggattat caatgtttga actataacta attgttatat gctttttgta gaactgcatc
    10321 ttactttgac atactttaag tcgttcatta agatatgacg attccaatga cgaactttta
    10381 ataacatcaa tttgtcggaa tgccttaatc atataaaata agcacaacaa actaccaaat
    10441 acccatatca aaagaatcat atacgttata tttgaggtct caaactgatt aacattaatt
    10501 gctaagtctt tcgtaacaga tgattgttga ccatctaaca tatgactaac cgaagaagtc
    10561 gtgtcagata catttcgatt catcatatct tttgaaaatg taaaattcga tattttgtaa
    10621 aatggtatta atggaattaa cgtggagacg agcactaata accaaatctt atgtgacata
    10681 atattttgag tatattttat atagagcatt ctcactaaaa aaattacaca tatcgtgagc
    10741 aatgaactga ttatacttaa cattaaaaaa gatgataaca ccttctacac ctccatatca
    10801 caaaaattat aacattattt tgacataaat actacatttg taatatacta caaatgtagt
    10861 cttatataag gagtatattg atgaaaaaga taaaaattgt tccacttatt ttaatagttg
    10921 tagttgtcgg gtttggtata tatttttatg cttcaaaaga taaagaaatt aataatacta
    10981 ttgatgcaat tgaagataaa aatttcaaac aagtttataa agatagcagt tatatttcta
    11041 aaagcgataa tggtgaagta gaaatgactg aacgtccgat aaaaatatat aatagtttag
    11101 gcgttaaaga tataaacatt caggatcgta aaataaaaaa agtatctaaa aataaaaaac
    11161 gagtagatgc tcaatataaa attaaaacaa actacggtaa cattgatcgc aacgttcaat
    11221 ttaattttgt taaagaagat ggtatgtgga agttagattg ggatcatagc gtcattattc
    11281 caggaatgca gaaagaccaa agcatacata ttgaaaattt aaaatcagaa cgtggtaaaa
    11341 ttttagaccg aaacaatgtg gaattggcca atacaggaac agcatatgag ataggcatcg
    11401 ttccaaagaa tgtatctaaa aaagattata aagcaatcgc taaagaacta agtatttctg
    11461 aagactatat caaacaacaa atggatcaaa attgggtaca agatgatacc ttcgttccac
    11521 ttaaaaccgt taaaaaaatg gatgaatatt taagtgattt cgcaaaaaaa tttcatctta
    11581 caactaatga aacagaaagt cgtaactatc ctctaggaaa agcgacttca catctattag
    11641 gttatgttgg tcccattaac tctgaagaat taaaacaaaa agaatataaa ggctataaag
    11701 atgatgcagt tattggtaaa aagggactcg aaaaacttta cgataaaaag ctccaacatg
    11761 aagatggcta tcgtgtcaca atcgttgacg ataatagcaa tacaatcgca catacattaa
    11821 tagagaaaaa gaaaaaagat ggcaaagata ttcaactaac tattgatgct aaagttcaaa
    11881 agagtattta taacaacatg aaaaatgatt atggctcagg tactgctatc caccctcaaa
    11941 caggtgaatt attagcactt gtaagcacac cttcatatga cgtctatcca tttatgtatg
    12001 gcatgagtaa cgaagaatat aataaattaa ccgaagataa aaaagaacct ctgctcaaca
    12061 agttccagat tacaacttca ccaggttcaa ctcaaaaaat attaacagca atgattgggt
    12121 taaataacaa aacattagac gataaaacaa gttataaaat cgatggtaaa ggttggcaaa
    12181 aagataaatc ttggggtggt tacaacgtta caagatatga agtggtaaat ggtaatatcg
    12241 acttaaaaca agcaatagaa tcatcagata acattttctt tgctagagta gcactcgaat
    12301 taggcagtaa gaaatttgaa aaaggcatga aaaaactagg tgttggtgaa gatataccaa
    12361 gtgattatcc attttataat gctcaaattt caaacaaaaa tttagataat gaaatattat
    12421 tagctgattc aggttacgga caaggtgaaa tactgattaa cccagtacag atcctttcaa
    12481 tctatagcgc attagaaaat aatggcaata ttaacgcacc tcacttatta aaagacacga
    12541 aaaacaaagt ttggaagaaa aatattattt ccaaagaaaa tatcaatcta ttaactgatg
    12601 gtatgcaaca agtcgtaaat aaaacacata aagaagatat ttatagatct tatgcaaact
    12661 taattggcaa atccggtact gcagaactca aaatgaaaca aggagaaact ggcagacaaa
    12721 ttgggtggtt tatatcatat gataaagata atccaaacat gatgatggct attaatgtta
    12781 aagatgtaca agataaagga atggctagct acaatgccaa aatctcaggt aaagtgtatg
    12841 atgagctata tgagaacggt aataaaaaat acgatataga tgaataacaa aacagtgaag
    12901 caatccgtaa cgatggttgc ttcactgttt tattatgaat tattaataag tgctgttact
    12961 tctcccttaa atacaatttc ttcattttca ttgtatgttg aaagtgacac tgtaacgagt
    13021 ccattttctt tttttatgga tttcttattt gtaatttcag cgataacgta caatgtatta
    13081 cctgggtata caggtttaat aaatttaacg ttattcattt gtgttcctgc tacaacttct
    13141 tctccgtatt taccttcttc tacccataat ttaaatgata ttgaaagtgt atgcatgcca
    13201 gatgcaatga tacctttaaa tctactttgt tctgcttttt ctttatctat atgcatatat
    13261 tgaggatcaa aagttgttgc aaattggata atttcttctt ctgtaatatg aaggcttttt
    13321 gttttgaatg tttctcctac tataaaatca tcgtatttca tatatgtctc tctttcttat
    13381 tcaaattaat tttttagtat gtaacatgtt aaaggtaagt ctaccgtcac tgaaacgtaa
    13441 gactcacctc taactttcta ttgagacaaa tgcaccattt tatctgcatt gtctgtaaag
    13501 ataccatcaa ctccccaatt agcaagttgg tttgcacgtg ctggtttgtt tacagtccat
    13561 acgttcaatt cataacccgc ttcttttacc atttttactt ttgctttagt aagtttggca
    13621 tcttcagtgt ttactatttt agcattacag taatctaaaa gtgttctcca gtcttcacga
    13681 aacgaagttg tatggaatat aactgctctg ttatattgtg gcatgatttc ttctgcaagt
    13741 ttaacaagca caacattaaa gcttgaaatg agcacttctt gattctgatt taagtttgtt
    13801 aattgttctt ccacttgctt aaccatactt ttagaaagtg ctagtccatt cggtccagta
    13861 atacctttta attctacatt taaattcata ttatattcat ttgctatttt tactacatca
    13921 tcgaaagttg gcaaatgttc atctttgaat ttttcaccaa accaagatcc tgcagaagca
    13981 tctttaattt catcataatt caattcagtt atttccccgg acatatttgt agtccgttct
    14041 aaataatcat catgaatgat aatcagttgt tcatcttttg taattgcaac atctaactcc
    14101 aaccagttta taccttctac ttctgaagca gctttaaatg atgcaattgt attttccgga
    14161 gctttactag gtaatcctct atgtccatat acagttagca tattacctct ccttgcattt
    14221 ttattttttt aattaacgta actgtattat cacattaatc gcacttttat ttccattaaa
    14281 aagagatgaa tatcataaat aaagaagtcg atagattcgt attgattatg gagttaatct
    14341 acgtctcatc tcatttttaa aaaatcattt atgtcccaag ctccattttg taatcaagtc
    14401 tagtttttct gtacccctta tctgcaattt tacttaggat tgcttttaac ttacccctta
    14461 tcagcaattt tactgagaac tgcttttaac gcacctctta tctgcaattt tgcctagaac
    14521 tgcttttaac gtacctctta tctgcaattt tactgagaac tgcttttaac ttacccctta
    14581 tcagcaattt tgcatggaat tgcttttaac gtacctctta tctgcaattt tacttagaac
    14641 tgcttttaac aaacctctta tctgcaattt tacttagaat tgcttttact attcctctta
    14701 ttagtataat ctcagtaaga atgcgtataa aaatgaaaat tacaaccgat tttgtaagtg
    14761 ctgacgcctg agggaatagt atgtgcgaga gactaatggc tcgagccata cccctaggca
    14821 agcatgcacg tacaaaatcg taagataaaa aaataagcat atcactgtaa actttaaaaa
    14881 atcagtttag tgatatgctt atttatttcg agttaggatt tatgtcccaa gctcatcaag
    14941 cacaatcggc cactagttta tttctctatc ttatatgttc tgatatggtc ttctatactg
    15001 tataagtata cttttgaata tggatcttgt gtcaattcac gttcgaaatc aaattcttga
    15061 ttatcaaatc tgttaaagaa tgtttcgtat tcttcgactg ataattgctc tctagattct
    15121 agcatattta agtgtttctc tttatctaat gctttgtcat atcctttaac gattgaacca
    15181 ctaaagattt ctcctactgc tcctgaacca taactaaata gacatacttt ctcttctggt
    15241 tggaatgtgt ggttctgtaa taacgaaatt aaacttaagt ataatgatcc tgtataaatg
    15301 ttaccaacat ctctattcca taatacggtt ctgttgcaaa gttgaattta tagtataatt
    15361 ttaacaaaaa ggagtcttct gtatgaacta tttcagatat aaacaattta acaaggatgt
    15421 tatcactgta gccgttggct actatctaag atatacattg agttatcgtg atatatctga
    15481 aatattaagg gaacgtggtg taaacgttca tcattcaacg gtctaccgtt gggttcaaga
    15541 atatgcccca attttgtatc aaatttggaa gaaaaagcat aaaaaagctt attacaaatg
    15601 gcgtattgat gagacgtaca tcaaaataaa aggaaaatgg agctatttat atcgtgccat
    15661 tgatgcagag ggacatacat tagatatttg gttgcgtaag caacgagata atcattcagc
    15721 atatgcgttt atcaaacgtc tcattaaaca atttggtaaa cctcaaaagg taattacaga
    15781 tcaggcacct tcaacgaagg tagcaatggc taaagtaatt aaagctttta aacttaaacc
    15841 tgactgtcat tgtacatcga aatatctgaa taacctcatt gagcaagatc accgtcatat
    15901 taaagtaaga aagacaaggt atcaaagtat caatacagca aagaatactt taaaaggtat
    15961 tgaatgtatt tacgctctat ataaaaagaa ccgcaggtct cttcagatct acggattttc
    16021 gccatgccac gaaattagca tcatgctagc aagttaagcg aacactgaca tgataaatta
    16081 gtggttagct atattttttt actttgcaac agaaccgaaa ataatctctt caatttattt
    16141 ttatatgaat cctgtgactc aatgattgta atatctaaag atttcagttc atcatagaca
    16201 atgttctttt caacattttt tatagcaaat tgattaaata aattctctaa tttctcccgt
    16261 ttgatttcac taccatagat tatattatca ttgatatagt caatgaataa tgacaaatta
    16321 tcactcataa cagtcccaac ccctttattt tgatagacta attatcttca tcattgtaaa
    16381 acaaattaca ccctttaaat ttaactcaac ttaaatatcg acaaattaaa aaacaataaa
    16441 attacttgaa tattattcat aatatattaa caactttatt atactgctct ttatatataa
    16501 aatcattaat aattaaacaa gccttaaaat atttaacttt tttgtgatta ttacacatta
    16561 tcttatctgc tctttatcac cataaaaata gaaaaaacaa gattcctaaa gaatatagga
    16621 atcttgtttc agactgtgga caaactgatt ttttatcagt tagcttattt agaaagtttt
    16681 atttaaatta cagtttctat ttttattaga tcacaatttt attttagctc ttgttcaagt
    16741 aatcattttt cgccaaaaac tttatactga atagcttcta cattaaatac tttgtcaatg
    16801 agatcatcta catctttaaa ttcagaataa tttgcatatg gatctataaa ataaaattgt
    16861 ggttctttac cggaaacatt aaatattctt aatattaaat atttctgctt atattctttc
    16921 atagcaaaca tttcatttag cgacataaaa aatggttcct caatactaga agatgtagat
    16981 gttttaattt caataaattt ttctacagct ttatctgtat ttgttggatc aaaagctact
    17041 aaatcatagc catgaccgtg ttgagagcct ggattatcat ttaaaatatt cctaaactgt
    17101 tctttcttat cttcgtctat tttattatca attagctcat taaagtaatt tagcgctaat
    17161 ttttctccaa ctttaccggt taatttattc tctttatttg atttttcaat ttctgaatca
    17221 tttttagtag tctttgatac acctttttta tattttggaa ttattccttt aggtgcttcc
    17281 acttccttga gtgtcttatc tttttgtgct gttctaattt cttcaatttc gctgtcttcc
    17341 tgtatttcgt ctatgctatt gaccaagcta tcataggatg tttttgtaac ttttgaagct
    17401 aattcattaa atagttctaa aaatttcttt aaatcctcta gcatatcttc ttctgtgaat
    17461 ccttcattca aatcataata tttgaatctt attgatccat gagaatatcc tgatggataa
    17521 tcatttttta aatcataaga tgaatcttta ttttctgcgt aataaaatct tccagtatta
    17581 aattcatttg atgtaatata tttattgagt tcggaagata aagttaatgc tctttgtttt
    17641 gcagcatttt tatcccgcgg aaacatatca cttatctttg accatccttg attcaaagat
    17701 aagtatatgc cttctccttc cggatgaaaa agatatacca aataatatcc atcctttgtt
    17761 tcttttgtta tattctcatc atatattgaa atccaaggaa ctttactata gttcccagta
    17821 gcaaccttcc ctacaactga atatttatct tcttttatat gcacttttaa ctgcttgggt
    17881 aacttatcat ggactaaagt tttatataga tcacctttat cccaatcaga ttttttaact
    17941 acattattgg tacgtttctc tttaattaat ttaaggacct gcataaagtt gtctatcatt
    18001 tgaaattccc tcctattata aaatatatta tgtctcattt tcttcaatat gtacttattt
    18061 atattttacc gtaatttact atatttagtt gcagaaagaa ttttctcaaa gctagaactt
    18121 tgcttcacta taagtattca gtataaagaa tatttcgcta ttatttactt gaaatgaaag
    18181 actgcggagg ctaactatgt caaaaatcat gaacctcatt acttatgata agcttcttaa
    18241 aaacataaca gcaattcaca taaacctcat atgttctgat acattcaaaa tccctttatg
    18301 aagcggctga aaaaaccgca tcatttatga tatgcttctc ctcgcataat cttaaatgct
    18361 ctgtacactt gctcaattaa tacaacccgc atcatttgat gtgggaatgt cattttgctg
    18421 aatgatagtg cgtagttact gcgttgtaag acgtccttgt gcaggccgtt tgatccgcca
    18481 atgacgaaaa caaagtcgct ttgcccttgg gtcatgcgtt ggttcaattc ttgggccaat
    18541 ccttcggaag atagcatctt tccttgtatt tctaatgtaa tgactgtgga ttgtggtttg
    18601 attttggcta gtattcgttg gccttctttt tcttttactt gctcaatttc tttgtcactc
    18661 atattttctg gtgctttttc gtctggaact tctatgatgt ctatcttggt gtatgggcct
    18721 aaacgttttt catattctgc tatggcttgc ttccaatatt tctcttttag tttccctaca
    18781 gctaaaatgg tgattttcat gtcgtttggt cctccaaatt gttatcaact ttccagttat
    18841 ccacaagtta ttaacttgtt cacactgttc cctcttatta taccaatatt ttttgcagtt
    18901 tttgatattt tcctgacatt tatgcccgat ttttagtgta taacctgtgt atttgtggat
    18961 aactctgtgg gcattgtggg tatttctctt taaattgtac acaattctgg ataactatcc
    19021 ccatttctgt ggataaacac cttgatgtct tattacttat cctcgttttt atatcccata
    19081 tttatcaacg gttatcgctt tttttcataa aaaaacacta ccgtacctct tatttaagaa
    19141 atacagtagt attattttca atatatttta gctagctttt ctacgtcgta cagtcgcgat
    19201 taaacctata cctgctaata atacaaacat cgcttctggg cttgaacttt gattagttcc
    19261 agtttttggt aactgtttct catgcgcgct acctttaggc actgacattc tagccaattg
    19321 tttcccactc ttgcttgata cagtagctcc ttctattgtg cgaccagaac cttctgtacc
    19381 gctactaaca gttcctctat gcgctagcaa caatacaact ttacctggag ctggtttttt
    19441 cgctggatcc atcactttgt cgtcaccaat tggttgtgca tctttaccag actcactacc
    19501 tttgctacct tgttgtcctt tagctggttg ttcacttact gctggtttac ctaataacat
    19561 acgttgtggt tctgtcgtat catacttagc taagttagct gtttttaaat aacttgctag
    19621 tacttgatct aatgaaatac cttcttctct agtaccaccg aacatactat atccgtcgcc
    19681 acctgatgct gtgaagtcat tcatcgttac gtgatataca cgttttaaat caatattttc
    19741 aaacttacct gtctctttat ttaaaatttg aatagcatta attcgtttgc cagacggttt
    19801 atttatatca tagtaaacac ggattgaatc agagatatgt agtaaaccgc cattcgctgt
    19861 taacactgtc ttaccgtcct tttgtgttgt tggtgcgcct aaactatgtt cgaaagccgt
    19921 ccagacgtct gaacctttta catcaatttg cgcaatcgta tttccaaatg gtaatactga
    19981 gattaaatca tagcgtgtca ccttaccttt tgcgatagag gcacgaatac ctccaccatt
    20041 tgtcacggca aagtcagtct ttttagagaa attcttaacg ccatatgctt ccatagcatc
    20101 tgcaattgcg tttcctaaat ttgtttcacg cgttctaacg tcatctctct ctcctttgaa
    20161 atcaatcgta ttatttggaa taataacctc tgctgtttgt gctctaaatg tttgatcagc
    20221 ttgattaatt tgttcagcta atgctttgtt cggtgtcaca ttttcaacgt ctttaacgtt
    20281 aatcaatgac ggtttaatat ttgatacctc tccattgcgg taattaaatg taatcttacc
    20341 gatattcgca agtgctgtac ctgtttgtgc taatgcatca ttgttataaa tttgaccatt
    20401 ttgaagtacg gtatgtgaat gaccatcaat gactgtaatt cgtttcttca attgtggatt
    20461 ttgacttaat tgtttcacta agtaatcacc acgccatgtt tcttgtgttg aaggatcaat
    20521 ccctaaatgt gatataacaa caaatgtatc tacgtcttta taaatacgca tcatttctgc
    20581 tgtcacactt tgtaatggat ctctaaattc aacaccttta atgccttcag gtcttgtttt
    20641 cgtctttgtt tctggtgtcg ttacgccaat aattccataa cgaataccat ttttcgttac
    20701 aatcgttgaa ggcttaaacg cgcgttttcc atctttataa acgttagtgc ttagcatcgg
    20761 gaagtctaac ataccctcta actttttcaa ctgatcgtat ccaaagtcaa attcgtggtt
    20821 accgactgcc atagcatcat aacctactgc attcattgct ttagccattt cttcaccttt
    20881 tgactggttt gaaagtggta aaccttggaa ggcgtctcct gcgtctaaca ttaaatcagg
    20941 cttttcttgt tcttttactg tttttaattt agccataccg atgacacgcc ctttttcttc
    21001 ggctagtcgg ccatggatat catttgtatg taatatttta tgtgtcgttg tttgtggtgc
    21061 attagcagca aacattcgtg gtgaaagtga tgctgtttta gcatttgata atttgaatgt
    21121 tgctgtgtga gttggttttt gtgttgaggc ttgttgtgta tctacgtcgc gcgtttcgtt
    21181 tactgttgtt gaaactgctg tagatggttt gttagatggt gcgttggatg ttactttgtt
    21241 gtttgtatca tcagctgttc cctgtgctga tgctgatacg ttggttgccg cttcttcacc
    21301 agattgatga gctgcttcct ttgttggcgg tacttgttgc ttatctgttg ttgctttgtc
    21361 tatcgttgtt actgcatgtg ctttcattgg tgtatgctgc tcagccgcgt tcgagacttg
    21421 ccataatccc attgcactaa aaagcaaaac gagccataca cttgttttaa gtaataaagc
    21481 tttcatttcg ttccctccat taactgtcat acaccaatat attatcataa tccttatatt
    21541 aaaatgtttg attttatgta aattatttat aaattacatt taatatcgaa ttattttaca
    21601 catttcaatc aagccggttc aatctaacaa cagccatttc ccaccaataa cataaaaaag
    21661 cacccgataa agtcgtctca cagcagtgcc gaactttata gggtgactct catttatatt
    21721 gtatatattg gcgttggaat agctttatcc gtatcacata gcaatacttc tttttccgta
    21781 tcaatatcgt gttcgttcaa tacttgacca acactcatac gcgccaaatc tttcatatta
    21841 ttatcttgtg ataaatgcga taggtaaata cgtttcgtat tacctgtaat cacgtctgtc
    21901 atcgcatgac ccgcatcctc attagataca tgacccatat cgcctaaaat acgttgtttc
    21961 gtcttccatg gataacgaca cattctcaac atatcgacgt catgattact ctcaaaaata
    22021 aacgcatcgc tgccacgtat catacctttc atacgatccg acacgtaacc cgtatccgtc
    22081 aaaatcgtaa acttcttata gttattatgg aaaatataaa attgcggatc tatcgcatca
    22141 tgtgacacgt taaacgattc aacatcgaaa cctgcaatag attttgtttc ataaggatta
    22201 aaaatgaatt tctgatccat agggatgcga ctatctttct tttcaattgc ctgccaagtc
    22261 ttttcattcg cataaattgg caattgatat ttacgcgcca aaacacctaa tcctttaata
    22321 tgatcaatat gttcatgggt tactaaaata ccatttaaat cttgaatatt acggtcaatt
    22381 tgactaaaca attcttccat tttctttcca gtcaaaccaa catcaactaa tagactacct
    22441 ttttcatttt ctacaaaagt ggcgttacct gtactaccac ttgctaatac actcatgcgt
    22501 atcaagcggc tcatcctttc tatttaagca gtcaatctct ctatatgact gcaatataca
    22561 tcagtgcaaa tatacgattt tccctacaga aactatcagt atattagctg ttttgtatac
    22621 ctttcctatt atgtaacttt agttcatcga ccggtctctt ataattaaat tttgataatc
    22681 acgtgcaatg aatgtcctaa ttattataac aatgattaga caaatactaa agtttttaaa
    22741 ataacagtta cactaatttt acaatgcggt tctacttaaa atatgaataa atgcgcacgt
    22801 tggagacgca aaaacatcag ccataataaa ccgtaccagc aaaaaagcta gcaccgcttc
    22861 tcacatatga tgagcttgca atgctagctt attacgattc atattaatga ttaataattt
    22921 tagggttatt atttgtcgct tcgacatagt aagtattcgt tttatcctta ccgtcatgct
    22981 tcactttaat ttcccagttt ggttgtaaca attgaacatt cgtttcattc acgactgagt
    23041 agtaaccgag tctagcatta atgacttcat cacctttttt caagtaacga ttataatata
    23101 atgcctcgat tgcttttctc acaccaatca cttgatgctt cttatctgca ccatctgtgg
    23161 gcttaatgtc atccatcatt gattgtttat aactagtcgc tttgttatct tctatattaa
    23221 agttcaacat cgctttactg ttatttaaaa tcggaaaatc atcatacgtt tgttcatatt
    23281 ttacagaatc tgaactaatc tcgcttaatt gatattcagc acctttaaat acgcgcttgt
    23341 taacataatc tttcaaatct tttaagttat tgtcactgac ctttaccgat tgactaatgt
    23401 tcgcattcaa taattttcca ccatctgatg tggtcaaatc cgaatcgcct ttagctttag
    23461 aactaaagtc ttttgatcgc cctgtaattt gctctaattt tataccttta actgatttat
    23521 tcaatatact agtcggtact ttaatttctt cttgctgaaa attaacttca ttgttactct
    23581 cgacttcatt aatgtgtgag cgattgactt tattaacata aatcgatact aacacgatgt
    23641 tgacaagaat aaacacgaaa atgaaaagtg tctttgtcag tttccagttc atttattcaa
    23701 gcctcccatc gttataaaca taccattcgc catcatattc tacataccaa cgcggtacga
    23761 gttcactgtt aatctgcact tcaatgtgat tatgatctga attatcctgc atttcgtaac
    23821 cgatagcgat gtttgttact ttttcaaaat taatatcact attgttcgct aagcttgaac
    23881 gtacagactc taatttcggc aacgatttat tatcctcact atttaaaaca acgtcggtgc
    23941 gtaataacga acgacgatag tcaaagacgc ctttttcacc ccaagtgact tgaatttgat
    24001 tagaaccttc tttattaaac gttggataac cattaaggaa acgttgatat gttaactcgc
    24061 ctgactgatt attcgtacta aacaatctaa agtcttcgtt taagaaacca ccatgaccat
    24121 taataaaatc aaaggttcct ggaatcgttt cttccatttt gctggaactc gcttcatctt
    24181 cggacaggtt tttataatga tatttttcat ttttatcgtt atagtttgcg acacctgtat
    24241 tattgttgta ggttgtaaca ccactctttg aactacgaac gatggttgaa tcgtcaaata
    24301 gtatagcatt cattttctca acactaatcg tgttaaatac catgcgatat gtttttaact
    24361 tttcaggttt acttggtgca aaaacatgcg tcgtacgatc aattgtatct ttgtttgtga
    24421 tgatatctgt gtatggttgc atatcttttt tcactgctgc taatgcatct aaaaaatgat
    24481 catttttcgt tgtagttgtt aattttacgt aatcgtggcg atctttgctt atagcataaa
    24541 gcacgatatt atcatcagca tcatgatcta tgaccaaacg attgaaattg aaatgatttg
    24601 gtactttcgc attcatgttc agtacttgac caagatatgt tgataacggt aaatcatacg
    24661 taaaatcgaa taatataaaa tcactgctca aatcaggaat catcaagtta tgatcacgac
    24721 gaacatgttc cacggacttc acttctttat ttttcaaagg tttcgtcagt ttattcacat
    24781 tagataccgt cgcaatcgtt ccttctggat gatcattttt cgaatgaata atctgaaatg
    24841 gcgtaatagt tgtatccatt ttggctgtca taggtgtcgt taaaggtttc gtttcactct
    24901 tcttactatc tgtattgtcg acatttgcaa tatcaggaga aaagttccat accatatatg
    24961 tcaatacgac actcatcaag acgagtagtg ctaaaatgac agatttaata tgctccttat
    25021 tattcatccc aatcaccgtc ttcaatgact tcacatggaa gtgtgataaa gatagatgta
    25081 ccttgacctt ctacactgtt tgcccaaata cgaccattgt gcgcttccac aatctctttc
    25141 gaaatggcta gtcctaatcc agtaccaccc attttacgcg tacgtgcctt atctacacga
    25201 tagaatcggt cgaatatctt atcgacttta ttgataggaa taccaatgcc attatcttta
    25261 atacgaatcg tcattcgatt ataaagtgga ttttgtttca cgtggaactc gacacgttta
    25321 tcgcctctag aatatttcat cgcatttgta atgacattat caaatacttg cgtcatttta
    25381 tcaggatcaa attctgtgaa aatcgtcttt ttcggaatat ctcgaataaa tgttgtatct
    25441 ttcgcagaca tttcatgtcg attaataatt ttattaatga acatgttaaa gtcgataatt
    25501 tctttgttga tttgatcaga ctcattatcc attttagata actgtagcaa gtcattgacc
    25561 agtcgaatca ttcgttctgt ttcttcacgg gtaacagata aaaattgtgg cgcaagttcc
    25621 tcatctttcc atgcaccttc ttcaagtgct tcaatgtaac tattcataga agttaaaggt
    25681 gtacgtaact catgtgatac attggcaaca aattcacgac gctcacgttc aacttgttgt
    25741 tgttcagtta cgtcatgtaa cacagcgata taaccagtta caaatcctgt ttcctgcaca
    25801 atcgtactaa agttaacacg tgcgattaga ccttcttctt catttaaatc taataagaaa
    25861 ctatcattat tctcttgaat ttcttccagt ttaaattcat cttcaagact taatacactt
    25921 aacatgtaat atccgatgat gtcttctttc gccataccaa gcatcttgag tgccatatca
    25981 ttgacgatac gaatacgtcc acggcggtct gttgcaataa taccatcact catatgggtg
    26041 ataactgagt ccagtctacg tttctcactt tcagtattag cctgcgcttc ttgtacacgt
    26101 ttagacaagt tattaaatgc taaagctaat tcgccaattt catcattacc ataaatcttc
    26161 acacgttgcg tatagttacc tctggacatt tcgaccgtct ggttacgcat atcggtgatt
    26221 ggtttggtaa tcgttcgcgc tataaagaat cctaggatga ctgtgattaa taatgaaata
    26281 gctgtaccaa caatgaatat ttgatttata ttatttaatt ggttataaac gtcattaatt
    26341 tttgattcga tataaatatt accaattacc tttttatcga ctttaactgg gatattatat
    26401 acccagacac ggtccttacc accgccataa tcttttaaaa ttaaatgatc gtttgattgt
    26461 cctagtgata gtgctttttg gacagaacta tcattcgttt tttgattgat tagactacgg
    26521 ttagactgct tcgtcgtcgc aataataatt tggtctttat ctataaaacg aatttctcca
    26581 atttcttgac ggttggcata ctcacttaat aaattttgaa tatctttttg tgcatttacg
    26641 gagccctttt cgtcatatac tttttcaata ctaatttcta attgtttcgc gtactgcgta
    26701 atattcttct taaaattatc aagcagctct ttttcaaggt tatttgtaaa atataacccg
    26761 ataatttgca taccaatgat aatcagtaat acataaacaa ttacaagttt agtatgaagg
    26821 gattgtagtt gttttagcca cttcattcgt ttcgacctct actcatgttg ttggaggaaa
    26881 tatccaacgc ctctacgcgt cacaatatat tcaggatgtg acggatcatc ttcaatcttt
    26941 tcacgtaaac gacgaatcgt tacatcgacc gtacgtacat cgccaaagta atcatagccc
    27001 catactgttt gtaataaatg ttcacgtgtc attacttgtc ccatatgttt tgataaataa
    27061 tggaacaatt caaattcacg atgtgttaat tcaatatctt cgccacgttt tttaatagaa
    27121 tatgcgtctg gataaatcac aatatcttta attgtgattt cattcgttac atttccagtg
    27181 tcttgtgctg gttgtgagta atgacgacgt aagttcgctt tcacacgtgc gattaattca
    27241 cgcgtactaa acggtttcgt tacatagtca tctgcaccta gttctaaacc aagcacttta
    27301 tcaatttctg aatctttagc agtaagcatt attattggca tttcgtattt tttgcgcact
    27361 tcacgacata cttccatacc atcacgacca ggtaacatga tatctagtaa tacgatgtct
    27421 ggttcttctt cataaattaa gtcgactgca tcattaccat cgtatgcaca gtacacatcg
    27481 tatccttctt tttttaagtt aaattctaaa atatcagcaa tcggtttttc atcatcaact
    27541 acaacaactt ttctagccat ttgcataaac ctcttttctt aaatcattaa tattatttta
    27601 tcattttttg ttctttatcg tttatgtttg tgtaaaaaat cacaggatgt cgcttactat
    27661 ttttgttatt gtgcgcattt tccgtcttat aacgtatagt aacctattat ataatttacc
    27721 atttataagc tattaattct attttccaat ataaacttat atattactat tttttattag
    27781 tttctcacgg cggattgtga caatgatatt taacacaatg atgaggtctc tattccaata
    27841 ataaaattag gataatggca acttctagtt gcacgtcatt gctcagaaaa taaatatcat
    27901 tattcgttat tcgttacttt aacaattgat gttatggagg actggtggca tatcacaacg
    27961 aagaaatatg ttgacagtag catatgtttg tacgtctaat aaagatttta ttgtctacgt
    28021 ttatctttca tatccaacca ctaagttact tgaggttcat acattttaaa gaaaatggac
    28081 gaacagataa atcaatatga ttatcattcg tccatatatc ttttattaat ataatattaa
    28141 acgcaaaaaa atgcctatcc tcttggatag gcatttgt
//
LOCUS       DAHHYJ010000016        27880 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000016 DAHHYJ010000000
VERSION     DAHHYJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27880
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.016"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            51..4361
                     /locus_tag="NBK22_000546"
     CDS             51..4361
                     /locus_tag="NBK22_000546"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCD2413752.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKT
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMVETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
     gene            4651..5118
                     /gene="rimP"
                     /locus_tag="NBK22_000547"
     CDS             4651..5118
                     /gene="rimP"
                     /locus_tag="NBK22_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCD2413753.1"
                     /translation="MSKITEQVEVIVQPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            5139..6314
                     /gene="nusA"
                     /locus_tag="NBK22_000548"
     CDS             5139..6314
                     /gene="nusA"
                     /locus_tag="NBK22_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCD2413754.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDIALEDADTTESTEEVNDVSVETNVEKESE"
     gene            6335..6619
                     /locus_tag="NBK22_000549"
     CDS             6335..6619
                     /locus_tag="NBK22_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCD2413755.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            6616..6933
                     /locus_tag="NBK22_000550"
     CDS             6616..6933
                     /locus_tag="NBK22_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCD2413756.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            6938..9055
                     /gene="infB"
                     /locus_tag="NBK22_000551"
     CDS             6938..9055
                     /gene="infB"
                     /locus_tag="NBK22_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCD2413757.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            9441..9791
                     /gene="rbfA"
                     /locus_tag="NBK22_000552"
     CDS             9441..9791
                     /gene="rbfA"
                     /locus_tag="NBK22_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCD2413758.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            9960..10877
                     /gene="truB"
                     /locus_tag="NBK22_000553"
     CDS             9960..10877
                     /gene="truB"
                     /locus_tag="NBK22_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCD2413759.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLENFKGVIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            10892..11863
                     /gene="ribF"
                     /locus_tag="NBK22_000554"
     CDS             10892..11863
                     /gene="ribF"
                     /locus_tag="NBK22_000554"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCD2413760.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMSDDKSRAKYLLAVDFGDEVAYNI"
     gene            11978..12247
                     /gene="rpsO"
                     /locus_tag="NBK22_000555"
     CDS             11978..12247
                     /gene="rpsO"
                     /locus_tag="NBK22_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCD2413761.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            12617..14713
                     /gene="pnp"
                     /locus_tag="NBK22_000556"
     CDS             12617..14713
                     /gene="pnp"
                     /locus_tag="NBK22_000556"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCD2413762.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKYDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVDF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            14949..16622
                     /locus_tag="NBK22_000557"
     CDS             14949..16622
                     /locus_tag="NBK22_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD2413763.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            16878..19247
                     /locus_tag="NBK22_000558"
     CDS             16878..19247
                     /locus_tag="NBK22_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD2413764.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVALENIKAWFGSFNEKMSERNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRITQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            19252..19965
                     /locus_tag="NBK22_000559"
     CDS             19252..19965
                     /locus_tag="NBK22_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2413765.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            19996..21261
                     /locus_tag="NBK22_000560"
     CDS             19996..21261
                     /locus_tag="NBK22_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD2413766.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLQEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVSVAEKAFLDTIYVLTKGGGK"
     gene            21261..22547
                     /locus_tag="NBK22_000561"
     CDS             21261..22547
                     /locus_tag="NBK22_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD2413767.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFVIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSFYLNLDQQVDSRLEIKK"
     gene            22547..23251
                     /locus_tag="NBK22_000562"
     CDS             22547..23251
                     /locus_tag="NBK22_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD2413768.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            23356..24183
                     /locus_tag="NBK22_000563"
     CDS             23356..24183
                     /locus_tag="NBK22_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCD2413769.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            24202..24594
                     /locus_tag="NBK22_000564"
     CDS             24202..24594
                     /locus_tag="NBK22_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2413770.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVFIF"
     gene            24628..25206
                     /gene="pgsA"
                     /locus_tag="NBK22_000565"
     CDS             24628..25206
                     /gene="pgsA"
                     /locus_tag="NBK22_000565"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCD2413771.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGFEYFYKGRDVFKQK"
     gene            25433..26584
                     /locus_tag="NBK22_000566"
     CDS             25433..26584
                     /locus_tag="NBK22_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCD2413772.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLANHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            26749..27792
                     /gene="recA"
                     /locus_tag="NBK22_000567"
     CDS             26749..27792
                     /gene="recA"
                     /locus_tag="NBK22_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCD2413773.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDAPKSLFDEE"
BASE COUNT        10215 a         3756 c         5452 g         8457 t
ORIGIN      
        1 gttcgaacac gtaaaagtaa tttctattta aggtggtaat tgtcttggca atgacagagc
       61 aacaaaaatt taaagtgctt gctgatcaaa ttaaaatttc aaatcaatta gatgctgaaa
      121 ttttaaattc aggtgaactg acacgtatag atgtttctaa caaaaacaga acatgggaat
      181 ttcatattac attaccacaa ttcttagctc atgaagatta tttattattt ataaatgcaa
      241 tagagcaaga gtttaaagat atcgccaacg ttacatgtcg ttttacggta acaaatggca
      301 cgaatcaaga tgaacatgca attaaatact ttgggcactg tattgaccaa acagctttat
      361 ctccaaaagt taaaggtcaa ttgaaacaga aaaagcttat tatgtctgga aaagtattaa
      421 aagtaatggt atcaaatgac attgaacgta atcattttga taaggcatgt aatggaagtc
      481 ttatcaaagc gtttagaaat tgtggttttg atatcgataa aatcatattc gaaacaaatg
      541 ataatgatca agaacaaaac ttagcttctt tagaagcaca tattcaagaa gaagacgaac
      601 aaagtgcacg attggcaaca gagaaacttg aaaaaatgaa agctgaaaaa gcgaaacaac
      661 aagataacaa cgaaagtgct gtcgataagt gtcaaattgg taagccgatt caaattgaaa
      721 atattaaacc aattgaatct attattgagg aagagtttaa agttgcaata gagggtgtca
      781 tttttgatat aaacttaaaa gaacttaaaa gtggtcgcca tatcgtagaa attaaagtga
      841 ctgactatac ggactcttta gttttaaaaa tgtttactcg taaaaacaaa gatgatttag
      901 aacattttaa agcgctaagt gttggtaaat gggttagggc tcaaggtcgt attgaagaag
      961 atacatttat tagagattta gttatgatga tgtctgatat tgaagagatt aaaaaagcga
     1021 caaaaaaaga taaggctgaa gaaaagcgtg tagaattcca cttgcatact gcaatgagcc
     1081 aaatggatgg tatacccaat attggtgcgt atgttaaaca ggcagcagac tggggacatc
     1141 cagccattgc ggttacagac cataatgttg tgcaagcatt tccagatgct cacgcagcag
     1201 cggaaaaaca tggcattaaa atgatatacg gtatggaagg tatgttagtt gatgatggtg
     1261 ttccgattgc atacaaacca caagatgtcg tattaaaaga tgctacttat gttgtgttcg
     1321 acgttgagac aactggttta tcaaatcagt atgataaaat catcgagctt gcagctgtga
     1381 aagttcataa cggtgaaatc atcgataagt ttgaaaggtt tagtaatccg catgaacgat
     1441 tatcggaaac gattatcaat ttgacgcata ttactgatga tatgttagta gatgcccctg
     1501 agattgaaga agtacttaca gagtttaaag aatgggttgg cgatgcgata ttcgtagcgc
     1561 ataatgcttc gtttgatatg ggctttatcg atacgggata tgaacgtctt gggtttggac
     1621 catcaacgaa tggtgttatc gatactttag aattatctcg tacgattaat actgaatatg
     1681 gtaaacatgg tttgaatttc ttagctaaaa aatatggcgt agaattaacg caacatcacc
     1741 gtgccattta tgatacagaa gcaacagctt acattttcat aaaaatggtt caacaaatga
     1801 aagaattagg cgtattaaat cataacgaaa tcaacaaaaa actcagtaat gaagatgcat
     1861 ataaacgtgc aagacctagt catgtcacat taattgtaca aaaccaacaa ggtcttaaaa
     1921 atctatttaa aattgtaagt gcatcattgg tgaagtattt ctaccgtaca cctcgaattc
     1981 cacgttcatt gttagatgaa tatcgtgagg gattattggt aggtacagcg tgtgatgaag
     2041 gtgaattatt tacggcagtt atgcagaagg accagagcca agttgaaaaa attgccaaat
     2101 attatgattt tattgaaatt caaccaccgg cactttatca agatttaatt gatagagagc
     2161 ttattagaga tactgaaaca ttacatgaaa tttatcaacg tttaatacat gcaggtgaca
     2221 cagcgggtat acctgttatt gcgacaggaa atgcacacta tttgtttgaa catgatggta
     2281 tcgcacgtaa aattttaata gcatcacaac ccggcaatcc acttaatcgc tcaactttac
     2341 cggaagcaca ttttagaact acagatgaaa tgttaaacga gtttcatttt ttaggtgaag
     2401 aaaaagcgca tgaaattgtt gtgaaaaata caaacgaatt agcagatcga attgaacgtg
     2461 ttgttcctat taaagatgaa ttatacacac cgcgtatgga aggtgctaac gaagaaatta
     2521 gagaactaag ttatgcaaat gcgcgtaaac tgtatggtga agacctgcct caaatcgtaa
     2581 ttgatcgatt agaaaaagaa ttaaaaagta ttatcggtaa tggatttgcg gtaatttact
     2641 taatttcgca acgtttagtt aaaaaatcat tagatgatgg atacttagtt ggttcccgtg
     2701 gttcagtagg ttctagtttt gtagcgacaa tgactgagat tactgaagta aacccgttac
     2761 cgccacacta tatttgtccg aactgtaaaa cgagtgaatt tttcaatgat ggttcagtag
     2821 gatcaggatt tgatttacct gataagacgt gtgaaacttg tggagcgcca cttattaaag
     2881 aaggacaaga tattccgttt gaaacatttt taggatttaa gggagataaa gttcctgata
     2941 tcgacttaaa ctttagtggt gaatatcaac cgaatgccca taactacaca aaagtattat
     3001 ttggtgagga taaagtattc cgtgcaggta caattggtac tgttgctgaa aagactgctt
     3061 ttggttatgt taaaggttat ttgaatgatc aaggcatcca caaaagaggt gctgaaatag
     3121 atcgactcgt taaaggatgt acaggtgtta aacgtacaac tggacagcat ccagggggta
     3181 ttattgtagt acctgattac atggatattt atgattttac gccgatacaa tatcctgccg
     3241 atgatcaaaa ttcagcatgg atgacgacac attttgattt ccattctatt catgataatg
     3301 tattaaaact tgatatactt ggacacgatg atccaacaat gattcgtatg cttcaagatt
     3361 tatcgggcat tgatccaaaa acgatacctg tagatgacaa agaagtcatg caaatattta
     3421 gtacacctga aagtttaggt gttactgaag atgaaatttt atgtaaaaca ggtacgtttg
     3481 gggttccaga attcggtaca gggttcgtgc gtcaaatgtt agaagataca aagccaacaa
     3541 cattttctga attagttcaa atctcaggat tatctcatgg tacagatgtg tggttaggca
     3601 atgctcaaga attaattaaa accggtatat gtgatttatc aagtgtaatt ggttgtcgtg
     3661 atgatatcat ggtttattta atgtatgctg gtttagaacc atcaatggct tttaaaataa
     3721 tggagtcagt acgtaaaggt aaaggtttaa ctgaagaaat ggttgaaacg atgaaagaaa
     3781 atgaagtgcc ggattggtat ttagattcat gtcttaaaat taagtacatg ttccctaaag
     3841 cccatgcagc agcatacgtt ttaatggcag tacgtatcgc atatttcaaa gtacatcatc
     3901 cactttatta ctatgcatct tactttacaa ttcgtgcgtc agattttgat ttaatcacga
     3961 tgattaaaga taaaacaagc attcgaaata ctgtaaaaga catgtattct cgctatatgg
     4021 atctaggtaa aaaagaaaaa gacgtattaa cagtcttgga aattatgaat gaaatggcgc
     4081 atcgaggtta tcgcatgcaa ccgattagtt tagaaaagag tcaggcgttc gaatttatca
     4141 ttgaaggcga tacacttatt ccgccgttca tatcagtgcc tgggcttggc gaaaacgttg
     4201 cgaaacgaat tgttgaagct cgtgacgatg gaccattttt atcaaaagaa gatttaaaca
     4261 aaaaagctgg attatctcag aaaattattg agtatttaga tgagttaggc tcattaccga
     4321 atttaccaga taaagctcaa ctttcgatat tcgatatgta aaatgaaata atcaaggtat
     4381 ttatttaatg cgtatggcgt agttaaagaa atacaaaatt gttgctggac ataaaattat
     4441 gcctgtattt cttttcaacg tcttacgagt ctattcgaat gtaatggtga aataaatgaa
     4501 caaactttta caagaatctc tgattaatag tgaagtcatt tgtttcaagc ataaacttat
     4561 gctataatta agttgcttaa aaattagtga actcaggcag aagagtggga gattcccgct
     4621 cttttctatt tgccaaaaag ggaggcctgt atgagtaaaa ttacagaaca agtagaagtg
     4681 attgttcaac caattatgga agacttgaat tttgaacttg tagacgttga atatgtcaaa
     4741 gagggtagag atcattttct tagaatctct attgataaag aaggtggcgt agatttaaat
     4801 gattgtacgc tagcttctga aaaaataagt gaagctatgg atgcaaatga tcctattcct
     4861 gaaatgtatt atttagacgt agcgtcacct ggtgcagaac gtccaattaa aaaagaacaa
     4921 gatttccaaa atgcaataac taaacctgta tttgtttctt tatatgtacc aattgaaggt
     4981 gaaaaggaat ggttaggcat tttacaagaa gtcaataatg aaacaattgt agtacaagtt
     5041 aaaatcaaag caagaacgaa agatatagag ataccgagag acaaaatagc aaaagcacgt
     5101 cacgcagtta tgatttaacg tgatgaggag gaaaaaacgt gtcaagtaat gaattattat
     5161 tagctactga gtatttagaa aaagaaaaga agattcctag agcagtatta attgatgcta
     5221 ttgaagcagc tttaattact gcatacaaaa agaattatga tagtgcaaga aatgtccgtg
     5281 tggaattaaa tatggatcaa ggtactttca aagttatcgc tcgtaaagat gttgttgaag
     5341 aagtatttga cgacagagat gaagtggatt taagtacagc gcttgttaaa aaccctgcat
     5401 atgaaattgg tgatatatac gaagaagatg taacacctaa agattttggt cgtgtaggtg
     5461 ctcaagcagc gaaacaagca gtaatgcaac gtcttcgtga tgctgaacgt gaaattttat
     5521 ttgaagaatt tatagacaaa gaagaagaca tacttactgg aattattgac cgtgttgacc
     5581 atcgttatgt atatgtgaat ttaggtcgta tcgaagctgt tttatctgaa gcagaaagaa
     5641 gtcctaacga aaaatatatt cctaacgaac gtatcaaagt atatgttaac aaagtggaac
     5701 aaacgacaaa aggtcctcaa atctatgttt ctcgtagcca tccaggttta ttaaaacgtt
     5761 tatttgaaca agaagttcca gaaatttacg atggtactgt aattgttaaa tcagtagcac
     5821 gtgaagctgg cgatcgctct aaaattagtg tcttctctga aaacaatgat atagatgctg
     5881 ttggtgcatg tgttggtgct aaaggcgcac gtgttgaagc tgttgttgaa gagctaggtg
     5941 gcgaaaaaat cgacatcgtt caatggaatg aagatccaaa agtatttgta aaaaatgctt
     6001 taagcccttc tcaagtttta gaagttattg ttgatgaaac aaatcaatct acagtagttg
     6061 ttgttcctga ttatcaattg tcattagcga ttggtaaaag aggacaaaac gcacgtctag
     6121 ctgctaaatt aaccggctgg aaaattgata ttaaatcaga aacagatgcg cgtgaagcgg
     6181 gtatctatcc agtagttgaa gcagaaaaag taactgaaga agatattgct ttagaagatg
     6241 ctgacacaac agaatcaacc gaagaggtaa atgatgtttc agttgaaaca aatgtagaga
     6301 aagaatctga ataataggtt ggagtgaagt atctatgaaa aagaaaaaaa ttccgatgcg
     6361 aaaatgtatt ctttcaaatg aaatgcatcc caaaaaagat atgattcgtg ttgttgttaa
     6421 taaagaaggc gaaatctttg cggatgttac tggaaagaaa caaggccgtg gcgcatatgt
     6481 ttctaaagat gttgctatgg ttgaaaaagc acaacaaaaa gaaattttag aaaaatattt
     6541 taaagcatct aaagagcaat tggatcctgt ttacaaagaa attattagat taatttatag
     6601 agaagagatc ccaaaatgag tatagatcaa atattaaact ttttgggatt agcaatgaga
     6661 gctggtaaag taaaaacagg tgaatcagtc attgttaatg agattaaaaa aggaaatttg
     6721 aagctcgtta ttgttgcaaa tgatgcgtct gataatacag ctaaattaat tacagataaa
     6781 tgtaagagtt acaaagttcc attcagaaag tttggaaatc gaaatgaatt gggaatagca
     6841 cttggaaaag gtgagcgtgt taatgtaggg attactgacc caggctttgc taaaaagttg
     6901 ctatcaatga tagatgaata tcataaggag tgattatatg agtaaacaaa gaatttacga
     6961 atatgcgaaa gaattaaatc taaagagtaa agagattata gatgagttaa aaagcatgaa
     7021 tattgaggtt tcaaatcata tgcaagcttt ggaagatgac caaattaaag cattagataa
     7081 aaagttcaaa aaagaacaaa agaacgacaa taaacaaagc actcaaaata atcaccaaaa
     7141 atcaaacaat caaaaccaaa ataaagggca acaaaaagat aacaaaaaga atcaacaaca
     7201 aaataataaa ggcaacaaag gcaataaaaa gaataataga aataataaga aaaataacaa
     7261 gaataataaa ccacaaaatc aaccagctgc tccaaaagaa ataccatcaa aagtgacata
     7321 tcaagaaggt attacagtag gcgaatttgc ggataaatta aatgttgaat catcagaaat
     7381 tatcaaaaaa ttattcttac ttggtattgt tgctaatatc aatcaatcat taaatcaaga
     7441 aacaatcgaa ttaattgccg atgattatgg cgttgaggtt gaagaagaag ttgtgattaa
     7501 tgaagaagat ttatcaatct acttcgaaga cgaaaaagat gatccagaag caattgagag
     7561 accagcagtt gtaacaatta tgggacatgt tgaccatggt aaaactactt tattagattc
     7621 aattcgtcat acaaaagtta cagcaggtga agcaggcgga atcactcaac atattggtgc
     7681 atatcaaatt gaaaacgatg gcaaaaaaat cactttctta gatacaccgg gacatgctgc
     7741 atttacaacg atgcgtgcgc gtggtgcaca agtaacagat attacaattt tagtagttgc
     7801 agctgacgat ggtgttatgc cacaaacaat tgaagcaatt aaccatgcta aagaagcaga
     7861 agtaccaatt attgttgcag taaataaaat tgataaacca acttcaaatc ctgatcgagt
     7921 tatgcaagaa ttaactgaat atggtttaat tcctgaagat tggggcggcg aaacaatttt
     7981 cgttccactt tctgcattaa gtggtgatgg tatcgacgat ttattagaaa tgataggatt
     8041 agttgcagaa gttcaagaac ttaaagcaaa tcctaaaaac cgtgctgttg gtacagttat
     8101 cgaagctgaa ttagataaat cacgtggtcc ttctgcatca ttattagtac aaaacggtac
     8161 attaaatgtt ggtgatgcga ttgtagttgg taatacttac ggccgtatcc gtgcaatggt
     8221 taatgactta ggtcaaagaa tcaaaacggc tggtccatca acgcctgttg aaattacagg
     8281 tattaatgat gtgccacaag ctggggatcg ctttgttgta tttagtgatg aaaaacaagc
     8341 tcgtcgtatt ggtgaatcaa gacacgaagc tagcattgta caacaacgtc aagaaagtaa
     8401 aaatgtttca ttagataacc tgtttgaaca aatgaaacaa ggtgaaatga aagatttaaa
     8461 cgttattatt aaaggtgatg ttcaaggttc tgttgaagct ttagctgcat cattaatgaa
     8521 aattgatgtt gaaggcgtaa atgttcgtat cattcataca gcggttggtg caattaatga
     8581 gtcagacgtg acacttgcta atgcctcaaa tggtattatc attggtttca atgttcgtcc
     8641 agacagtggt gcaaaacgtg ctgcagaagc tgaaaatgtt gatatgcgtt tacacagagt
     8701 tatttataat gttatcgaag aaattgaatc agcgatgaaa ggtttacttg atccagaatt
     8761 tgaagaacaa gttatcggac aagctgaagt tcgtcaaaca ttcaaagttt ctaaagttgg
     8821 tactattgct ggatgttatg ttactgaagg taaaattacg cgaaatgctg gtgtacgtat
     8881 tatccgtgat ggtattgttc aatatgaagg cgaattagat acacttaaac gtttcaaaga
     8941 tgatgctaag gaagttgcaa aaggttatga atgtggtatt acaattgaaa actacaatga
     9001 ccttaaagaa ggcgatgtta tcgaagcatt tgaaatggtt gaaattaagc gttaattaaa
     9061 taaattacaa gctaaaagta tagttaagat tgatatgctc cctataaata ttgcactttt
     9121 taagtgtcta ctttataggg agcatatttg atactagctt ttggtttttt atttgaatag
     9181 attacctatt aaaagttacg ttatatggac atgattttgt ataaaatttt gtggtggcct
     9241 agaatgattt ttaatgacaa aatataatgt cgactattat tggaaaattt tctgttgaaa
     9301 tgcctatctt acggcaaact ttatttgatt ttataggctt aatttattaa aataacgtgt
     9361 gagctaaaat aattgtttaa gcattgttac actaaaaaat gcaaataaca attgaactta
     9421 aagataaaga ggtgacaaga atgagcagta tgagagcaga gcgtgttggt gaacaaatga
     9481 agaaggaatt aatggatatc atcaacaata aagtcaaaga tcctcgagtt ggttttatta
     9541 caattacaga tgttgtttta acaaatgatt tatcgcaggc taaagtattt ttaactgtat
     9601 taggtaacga taaagaagta gaaaatacat ttaaagcact tgataaagca aaaggcttca
     9661 ttaagtctga attaggttct agaatgcgat tacgtattat gccggaatta atgtatgaat
     9721 atgatcaatc aatcgaatat ggtaataaaa ttgaacgaat gattcaagat ttacacaaac
     9781 aagatagata atttagtgtt aggtatctgg aaaatgtttg ataatttctt aatatcggta
     9841 tattaacatt aaacagttaa tacatagatg tgtagaaata gttaacattt tccagttttt
     9901 ttatgaataa attcagttga tacgctatta aaatatattt taaaaaagaa ggtgactata
     9961 tgtataatgg gatattacca gtatataaag agcgcggttt aacaagtcat gacgttgtat
    10021 tcaaattgcg taaaatatta aaaactaaaa aaataggtca cacgggtacg cttgatcccg
    10081 aagttgcagg cgtgttaccg gtatgtatag gtaatgcaac gagagttagt gattatgtta
    10141 tggatatggg caaagcttat gaagcaactg tatcgatagg aagaagtaca acgactgaag
    10201 atcaaacggg tgatacattg gaaacaaaag gtgtacactc agcagatttt aataaggacg
    10261 atattgaccg attgttagaa aattttaaag gtgttattga acaaattccg ccaatgtatt
    10321 catcagtcaa ggtgaatggt aaaaaattat atgaatatgc gcgtaataat gaaacagttg
    10381 aaagaccaaa gcgtaaagtt aatattaaag acattgggcg tatatctgaa ttagatttta
    10441 aagaaaatga gtgtcatttt aaaatacgcg tcatctgtgg taaaggtaca tatattagaa
    10501 cgctagcaac tgatattggt gtgaaattag gctttccggc acatatgtcg aaattaacac
    10561 gaatcgagtc tggtggattt gtgttgaaag atagccttac attagaacaa ataaaagaac
    10621 ttcatgagca ggattcattg caaaataaat tgtttccttt agaatatgga ttaaagggtt
    10681 tgccaagcat taaaattaaa gattcgcaca taaaaaaacg tattttaaat gggcagaaat
    10741 ttaataaaaa tgaatttgat aacaaaatta aagaccaaat tgtatttatt gatgatgatt
    10801 cagaaaaagt attagcaatt tatatggtac accctacaaa agaatcagaa attaaaccta
    10861 aaaaagtctt taattaaagg agatagaatt tatgaaagtc atagaagtga cacatcctat
    10921 acaatctaaa cagtatatta cagaggatgt tgcaatggca ttcggatttt tcgatggcat
    10981 gcataaaggt catgacaaag tctttgatat attaaatgaa atagctgagg cacgcagttt
    11041 aaaaaaagcg gtgatgacat ttgatccgca tccatctgtc gtgttgaatc ctaaaagaaa
    11101 acgaacaacg tatttaacgc cactttcaga taaaatcgaa aaaattagcc aacatgatat
    11161 tgattattgt atagtggtta atttttcatc taggtttgct aatgtgagcg ttgaagattt
    11221 tgttgaaaat tatataatta aaaataatgt aaaagaagtc attgctggtt ttgattttac
    11281 ttttggtaaa tttggaaaag gtaatatgac tgttcttcaa gaatatgatg cgtttaatac
    11341 gacaattgtg agtaagcaag aaattgaaaa tgaaaaaatt tctacaactt ctattcgtca
    11401 agatttaatc aatggtgagt tgcaaaaagc gaatgatgct ttaggctata tatattctat
    11461 taaaggcact gtagtgcaag gtgaaaaaag aggaagaact attggcttcc caacagctaa
    11521 cattcaacct agtgatgatt atttgttacc tcgtaaaggt gtttatgctg ttagtattga
    11581 aatcggcact gaaaataaat tatatcgagg ggtagctaac ataggtgtaa agccaacatt
    11641 tcatgatcct aacaaagcag aagttgtcat cgaagtgaat atctttgact ttgaggataa
    11701 tatttatggt gaacgagtga ccgtgaattg gcatcatttc ttacgtcctg agattaaatt
    11761 tgatggtatc gacccattag ttaaacaaat gagcgatgat aaatcgcgtg ctaaatattt
    11821 attagcagtt gattttggtg atgaagtagc ttataatatt tagagttgcg tatagttata
    11881 taaacaatct ataccacacc tttttcttag taggtcgaat ctccaacgcc taactcggat
    11941 taaggagtat tcaaacattt taaggaggaa attgattatg gcaatttcac aagaacgtaa
    12001 aaacgaaatc attaaagaat accgtgtaca cgaaactgat actggttcac cagaagtaca
    12061 aatcgctgta cttactgcag aaatcaacgc agtaaacgaa cacttacgta cacacaaaaa
    12121 agaccaccat tcacgtcgtg gattattaaa aatggtaggt cgtcgtagac atttattaaa
    12181 ctacttacgt agtaaagata ttcaacgtta ccgtgaatta attaaatcac ttggtatccg
    12241 tcgttaatct taatataacg tctttgaggt tggggcatat ttatgttcca accttaattt
    12301 atattaaaaa agctttttac aaatattaac atttattata tgttaagcta atattgagtg
    12361 aataataagg ttataatgag ataaagatga tataagtaca cctagagtaa taatcaagat
    12421 attaaaaata aagtatgttt ttttaaaaaa tataacttat atttatactg ataagggtgg
    12481 gacgataagt ctattttgta aataatagat ggatatcccg ctctcttttt ttccaattca
    12541 atattttata actaatatta aaatacgata ataaatgata tgatataact attagattca
    12601 agagaggaga tttataatgt ctcaagaaaa gaaagttttt aaaactgaat gggcaggaag
    12661 atctttaacg attgaaacag ggcaattagc taaacaagca aatggcgctg tattggttcg
    12721 ttatggagat acagtcgtgt tatcgacggc aactgcatca aaagaacctc gtgatggaga
    12781 tttcttccca ttaacagtga actatgaaga aaaaatgtac gctgcgggta aaattcctgg
    12841 tggatttaaa aagagagaag gacgtcctgg tgacgatgca acattaactg cgcgattaat
    12901 tgatagacca attagacctt tattccctaa aggatataag tatgatgttc aaattatgaa
    12961 catggtatta agtgcagatc ctgattgttc accacaaatg gctgcaatga ttggttcatc
    13021 tatggcgctt agtgtgtcgg atattccatt ccaagggcca atcgccggtg taaatgtggg
    13081 ttatattgac ggtaaatata tcattaaccc aacagtagaa gaaaaagaag tttctcgttt
    13141 agaccttgaa gtagctggtc ataaagatgc agtaaacatg gtagaggcag gcgctagtga
    13201 gattactgaa caagaaatgt tagaggcgat tttctttggt catgaagaga ttcaacgttt
    13261 agttgatttc caacaacaaa tcgtcgacca cattcaacct gttaaacaag aatttattcc
    13321 agcagagcgt gatgaagcgc tagttgaacg tgtaaaatct ttaaccgaag aaaaaggact
    13381 taaagaaaca gttttaacat ttgataaaca acaacgagat gaaaatcttg ataacttaaa
    13441 agaagaaatc gtcaatgaat ttatcgatga agaagatcca gagaatgaat tacttattaa
    13501 agaagtttat gcaattttaa atgaattagt gaaagaagaa gttagacgtt taattgcaga
    13561 tgaaaaaatt agaccagacg gccgtaaacc tgatgaaatc cgtccattag attctgaagt
    13621 tggtatttta cctagaacgc atggttcagg tctatttaca cgtggtcaga ctcaagcact
    13681 ttcagtttta acattaggtg ctttaggcga ttatcaatta attgatggtt taggacctga
    13741 agaagaaaaa agattcatgc atcattacaa cttcccgaat ttttcagtag gtgaaactgg
    13801 tccagtacgt gcgccaggtc gtcgtgaaat tggacatggt gcgttaggtg aaagagcatt
    13861 aaaatatatt attcctgata ctgctgattt cccatataca attcgtattg taagtgaggt
    13921 acttgaatca aatggttcat catctcaagc gtcaatttgt ggatcaacat tagcattaat
    13981 ggatgcgggc gtaccgatta aagcaccagt tgctggtatt gctatgggcc ttgttacacg
    14041 tgaagatagc tatacgattt taactgatat ccaaggtatg gaagatgcat taggtgatat
    14101 ggactttaaa gtcgctggta ctaaagaagg tattacagca atccaaatgg atattaaaat
    14161 tgacggttta acgcgtgaaa ttatcgaaga ggctctagaa caagcgagac gtggtcgttt
    14221 agaaataatg aatcatatgt tacaaacaat tgatcaacca cgtactgaat taagtgctta
    14281 cgcgccaaaa gttgtaacta tgacaattaa accagataag attagagatg ttatcggacc
    14341 tggtggtaaa aaaattaacg aaattattga tgaaacaggc gttaaattag atattgaaca
    14401 agatggtact atctttattg gggccgttga tcaagctatg ataaatcgtg ctcgtgaaat
    14461 cattgaggaa attacacgtg aagcggaagt aggtcaaact tatcaagcca ctgttaaacg
    14521 tattgaaaaa tacggtgcgt ttgtaggcct attcccgggt aaagatgcgt tgcttcacat
    14581 ttcacaaatt tcaaaaaata gaattgaaaa agtggaagat gtattaaaaa tcggtgacac
    14641 aattgaagtt aagattactg aaattgataa acaaggtcga gtaaatgctt cacatagagc
    14701 attagaagaa taatatttaa agtcatatga cgacaatgta tcgtcatgtg atttttttat
    14761 gccatttttt acgaagtgat ccattttgaa tttgttgtat tgaacatttt aaaacgcttt
    14821 attattttgt gtgcaactgt taattatcct gtatgtatag tgattaatag tgtacatcaa
    14881 gtgtttttta acttataatg aatagtgagt ttatatatgg acgggtaaca aatttaggag
    14941 gtaagatttt gagtttaata aagaaaaaga ataaagatat tcgcattata ccattaggcg
    15001 gtgttggcga aattgctaaa aatatgtata tcgttgaagt agacgatgaa atgtttatgt
    15061 tagatgctgg acttatgttt ccagaagacg aaatgctagg tattgatatt gttataccag
    15121 acatttcata cgtacttgaa aataaagata aattgaaggg tatattcctt acacacggac
    15181 atgagcacgc gattggtgca gtgagttatg ttttagaaca attagatgca ccagtatatg
    15241 gatctaaatt gacaatagcg ttaattaaag aaaatatgaa agcccgtaat attgataaaa
    15301 aagttcgcta ctacacagtt aataatgatt caattatgag attcaaaaac gtgaatatta
    15361 gtttctttaa tacgacacac agtattcctg atagtctagg tgtttgtatt catacttcat
    15421 atggtgccat tgtgtataca ggtgaattta agtttgacca aagtttacat ggacattatg
    15481 caccagatat taaacgtatg gcagagattg gtgaagaagg cgtatttgtc ttaatcagtg
    15541 attctactga ggcagagaaa cctggatata atactccgga aaatgtgatt gaacatcata
    15601 tgtatgacgc ttttgcaaaa gtgcgaggtc gcttgattgt ttcatgttat gcttcgaact
    15661 ttatacgtat tcagcaagtt ttaaatattg ctagcaagct aaatcgtaaa gtgtcatttt
    15721 taggaagatc acttgaaagt tcatttaata ttgctcgtaa aatggggtat ttcgacattc
    15781 ctaaagattt gctaattcct ataacagaag ttgataatta tcctaaaaat gaagtgataa
    15841 ttatagctac tggtatgcaa ggagaacctg tagaagcctt aagtcaaatg gcgcaacata
    15901 agcataaaat tatgaatatc gaagaaggcg attctgtatt tttagcaatt acggcttctg
    15961 ctaatatgga agttatcatt gcgaatacat taaatgagct tgtacgtgct ggcgcacata
    16021 ttattccaaa taacaagaag attcatgctt caagtcatgg ttgcatggaa gaattaaaaa
    16081 tgatgattaa tattatgaaa cctgaatact ttattcctgt acaaggtgaa tttaaaatgc
    16141 agatagcaca tgcgaagcta gcagctgaag caggtgttgc accagaaaag attttccttg
    16201 tggaaaaagg agatgtcatt aattacaacg gtaaagatat gatattaaat gaaaaggtaa
    16261 attcaggaaa tattttaata gatggtattg gtattgggga tgtaggaaat atcgtgttga
    16321 gagaccgtca tcttttagca gaagatggta tctttattgc tgtagtaacg ttagatccta
    16381 aaaatagacg tatagctgcg ggacctgaaa ttcaatctcg tgggtttgta tatgtacgtg
    16441 aaagtgaaga cttattacgt gaagcagaag agaaagtacg tgaaatagta gaggctggtt
    16501 tacaagaaaa acgcatagaa tggtctgaaa ttaagcaaaa tatgcgtgat caaattagta
    16561 aactattatt cgaaagtaca aaacgtcgtc ctatgattat tccagtaatt tctgaaattt
    16621 aatcaaaaag tcattaacat aaaagaggtc agaacaagtc actgaaatat aatggttgtc
    16681 atggacaatt tacttatatt ttatgatggt caattgaagg ggtaacgatt aatctgttat
    16741 cttaagtaaa ttgatacata gatgatattg ttctaacctc tttcatcgtc tgtttggact
    16801 acatattcta aacatcaaat aggaaattat atataataac gtcgttttaa ctaaggcaac
    16861 ataaggaggt gcgtcaattg gcacaagcaa aaaagaaatc gacagctaag aaaaaaacaa
    16921 catcaaaaaa aagaacaaat tcgaggaaaa agaagaatga taatccgata cgttatgtca
    16981 tagctatttt agtagttgta ttaatggtgt tgggtgtttt ccaattagga ataataggtc
    17041 gtctaattga cagcttcttt aattatttat ttgggtacag tagatattta acatatattt
    17101 tagtactctt agcaactggt tttattacat actctaaacg tattcctaaa actagacgaa
    17161 cggctggttc gattgtattg caaattgcat tgctatttgt atcacagtta gtttttcatt
    17221 ttaatagtgg tatcaaagct gaaagagaac ctgtactttc ttatgtgtat cagtcatacc
    17281 aacacagtca tttcccaaat tttggtggcg gtgtattagg cttttattta ttagagttaa
    17341 gcgtaccttt aatttcatta tttggtgtat gtattattac tattttatta ttatgctcaa
    17401 gtgttatttt attaacaaac catcaacatc gtgaagttgc aaaagttgca ctggaaaata
    17461 taaaagcttg gtttggttca tttaatgaaa aaatgtcgga aagaaaccaa gaaaaacaat
    17521 tgaagcgtga agaaaaagca agacttaaag aagaacaaaa ggcacgtcaa aatgaacagc
    17581 cacaaataaa agatgtgagt gattttacgg aagtgcctca agaaagagat attccaattt
    17641 atgggcatac tgaaaatgaa agtaaaagcc agagtcaacc aagtcgaaaa aaacgagtgt
    17701 ttgatgcaga gaatagttcg aataacatcg taaatcatca tcaagcagat cagcaagaac
    17761 aattaacaga acaaactcat aacagtgttg aaagtgaaaa cactattgaa gaagctggtg
    17821 aagttacgaa tgtatcgtat gttgttccac cgttaacttt acttaatcaa cctgcaaaac
    17881 aaaaagcaac atctaaagct gaagtgcaac gtaaaggaca agtactagag aatacattaa
    17941 aagattttgg ggtaaatgca aaagtgacac aaattaaaat tggtcctgca gtaactcaat
    18001 atgaaattca accagctcaa ggggttaaag tgagtaaaat tgtaaacttg cataatgata
    18061 ttgcattagc tttagcagca aaagatgtta gaatcgaagc gccaatacct ggtcgttctg
    18121 cagtaggtat tgaagtgcca aatgagaaaa tttcattagt ttcactaaaa gaagttttag
    18181 atgaaaaatt cccgtctaat aataaactag aagttggatt aggaagagat atatcaggtg
    18241 atccaattac tgttccacta aatgaaatgc cacacttatt ggtggcagga tcgacgggta
    18301 gtggtaaatc tgtttgtata aatggtatta ttacaagtat tttattaaat gctaagccgc
    18361 atgaagttaa acttatgtta atcgatccga aaatggttga actaaatgtt tataacggaa
    18421 ttccacactt attaattccg gttgttacaa atcctcataa agctgctcaa gctttagaaa
    18481 aaattgtagc tgagatggaa agacgttatg atttattcca acattcatca actagaaaca
    18541 ttaaaggtta taacgaatta atccgtaagc aaaatcaaga attagatgag aagcaaccag
    18601 aattacctta tatcgttgtt attgtagatg agcttgcaga tttaatgatg gtagctggta
    18661 aagaagttga aaatgcgatt caacgtatta cacaaatggc acgtgcagca ggtatacatt
    18721 taattgtagc gacacaaaga ccttctgtgg atgtaattac aggtatcatt aaaaataata
    18781 ttccatctag aatagctttt gctgtgagtt ctcaaacaga ttcaagaact attattggta
    18841 ctggcggcgc agaaaagtta cttggtaaag gtgacatgtt atacgttgga aatggtgact
    18901 catcacaaac acgtattcaa ggggcgtttt taagtgacca agaggtgcaa gatgttgtaa
    18961 attatgtagt agaacaacaa caggcaaatt atgtaaaaga aatggaacca gatgcaccag
    19021 tggataaatc ggaaatgaaa agtgaagatg ctttatatga tgaagcgtat ttgtttgttg
    19081 ttgaacaaca aaaggcaagt acatcattgt tacaacgcca atttagaatt ggttataata
    19141 gagcatctag gttgatggat gatttagaac gcaatcaggt aatcggtcca caaaaaggaa
    19201 gcaagcctag acaagtttta atagatctta ataatgacga ggtgtaaaaa aatgtcagaa
    19261 atgaatgcgg tatataacgt taaacaatac attttaaatt tgattaagca aaataaattg
    19321 gaatatggtg accaacttcc aagtaattta tcaattgcca gagaattaaa tgtaaaaacc
    19381 gacgatgttt atgaagcaat tcaggcattg attactgaac aagtcattaa agataatttt
    19441 gaagagggca caagtgttaa gtcactgccc cctttctttt atccattgaa tgaacttata
    19501 agtattgggc aaatgattaa aaatgcagga tttgaatgcg gaactgaata cttaaatttt
    19561 gatgagcaac cagcaactat gttagatgca aatttgttga gcgttgaaga aggatatcca
    19621 gtaaccatta tagaacgatt acgaactgcc gatggagaac cggtcgtcta ttgtttagat
    19681 aaaattgcta aaaaagaatt aacatgtaca gagtatcaaa tgagcaatgg atcgattcta
    19741 agtgcgataa aagaacaaag taatcataat atttgttatg ctgatacaga aattgaagcg
    19801 gtaaattatg aacctcgaat atccgaagta ctgaatgctt cgccacacga aggtttgatt
    19861 ttattaaaaa ttacgcacta taatgaatca gatgaaccta ttttgtattc attaaattat
    19921 atgaaaaata gcttagttca atttaaaatc actagaaaaa tatagatgta agaagctata
    19981 gaggaggcta tatatttgag tagacaatct caaccaaata tacatatcaa agtttcacca
    20041 acaactaaat ttaaaacaac tactatagtt tttaaattta tggcaccttt agaatatgac
    20101 acaattacag ctagatcatt attaagcaaa ttattagttc gagcaactaa gaaatggcca
    20161 accgataagt cgtttaataa tcacttagcc gatttatatg gtgcatatgt gaatagtaca
    20221 atttcaaaat tcaaagatca gcatgtcatt acattttcat tagaaattgt aaatgaacgt
    20281 tatttaagaa acggtgaatc attatttaat caaggattag atttattaca agaaatcatt
    20341 tggaatccat taattgaaaa taaagcattc aatgataatt ttgttaacca agagaaaaca
    20401 ttattagcca aaaaaataga agcaatggta gataacaaag cacaatattc gtttttaaaa
    20461 ttactcgacc atatgtttga aaatgaagca tacaaatacc tatctacagg acaactagaa
    20521 caaatcccac atattactgc tgaaacacta tatcatacat atcaatcaat gattaataat
    20581 gatcaatgtt ctgtttatgt tgtcggcaat gtagaacctg aaagtgttga gaaacaaata
    20641 cgtgaaaaat ttgcacttaa accattcgat aaacatcaat tccaacattc tacacatcat
    20701 ttacacgatg aagaagttga ttatattgtt gaatatgatg acgtggatca agctaaatta
    20761 aatatgggat atcgttttcc aacacaatat ggacaaagtg gatatgctgc ttttgttgta
    20821 tttaacatga tgtttggagg agatccttca tctgttttat ttaatgaagt gcgagaaaag
    20881 caaagtttag cgtactctat acattcacaa attgatggca aaaatggcta tttatttgtt
    20941 ttgagtgggg tttcaagtga taagtacgaa actgcaaaag acactattat aagtgaattt
    21001 gaaaaaataa aagcaggaga tttcactgaa gaaaaattag agttagctaa aaaagtaatc
    21061 atttctcatc gatatgaatc tgaagatcgt ccgaaaagta ttatagagat tatgcataac
    21121 caaatattat tagagcaacc acaaagcaaa gaaacattta taaatgatat acagaaggta
    21181 agtcgcgaag atattgtttc tgttgctgaa aaagcatttt tagatacaat ctatgtgttg
    21241 acaaaaggag ggggtaaata atgaaagagc gttattatga attaatagac gaaagagtat
    21301 tcgaacaaga attagaaaat ggtttacgat tatttgttat ccctaaacca ggttttcaaa
    21361 agacatttgt cacttacact acacaatttg gttcattaga taatcaattc aaacccctcg
    21421 gacaagacca atttgttact gtacctgatg gagttgctca ctttttagaa cataaattat
    21481 ttgaaaaaga agaagaagac ttatttactg cgtttgctga agataacgca caagcaaatg
    21541 cgtttacaag ctttgatcgt acaagctact tgttcagtgc aactgataat attgaaaaca
    21601 acattaaacg tttacttaca atggttgaaa cgccttattt tacaaaagaa actgttgata
    21661 aagaaaaagg tattattgca gaagaaataa aaatgtatca agagcaacct ggatataaat
    21721 taatgtttaa tacattgcgt gcaatgtatc aacaacatcc aatacgtgtt gatattgccg
    21781 gtagtgtaga aagtatatac gatattacaa aagatgattt gtatctatgt tatgaaacgt
    21841 tttatcatcc atcaaatatg gttttatttg ttgttggcga tgtggatcct gaagcaatat
    21901 gtcgaatagt aaaacaacac gaggatgctc gtaataaagt taaccaaccc aaaatcgaac
    21961 gaggacttgt tgatgaaccg gaggatgtta aagaagcatt tgttactgaa tctatgaaaa
    22021 ttcaatcacc aagactaatg cttggtttta aaaataaacc attacaagaa gcgcctcaaa
    22081 aatatgtaca acgtgattta gaaatgtcat tattctttga gttaattttt ggggaagaaa
    22141 cagattttta tcagaattta ttaaacgaag gacttatcga tgatacattt ggttatcaat
    22201 ttgtactaga gccgacttat agtttttcaa tcgtgacaag cgctactgaa gaaccagata
    22261 aattaaaaaa attattatta gatgagttgc gtgataaaaa aggcaatttc caagatgcag
    22321 aagcatttga acttttgaaa aagcaattta taggtgaatt catatcaagt ttaaactcac
    22381 ctgaatatat tgctaatcaa tatactaaat tgtattttga aggtgttagc gtgtttgaca
    22441 tgttagacat tgttgaaaat atcaccttag atagtattaa cgaaacgtca tcgttctatt
    22501 taaatttaga tcagcaagtc gatagtcgtt tggagattaa aaagtaatga aagcattagt
    22561 attaggtggt tctggttcaa ttggttctga gatagtcaaa caattattaa ctgatggatt
    22621 tgaagtttat gtgcaatatt atcgtactga tataaatgaa ttaactagca aatttaatga
    22681 tgataaagtt cgttttatac aagcggattt atctcaaaca attgatattg acaagacatt
    22741 tggtgacatt aaatcattag actgtttaat atatgcaagt ggtcagtctt tatatggtgt
    22801 tttacaagat atgaaagacc atgatattga tgcatgttat cagttaaatg tcttgcaatt
    22861 aattcgatta tgtagatatt tcgttgatgt tttacgtcaa agtgccaatg gaagaattat
    22921 tgtaatttca tcaatttggg gtgagacagg agctagtatg gaaactattt attcggcgat
    22981 gaaaagtgca caattaggtt tcgttaaggc gcttagtcaa gagcttgcac taacatcagt
    23041 gacagtaaat gctatcgcac ctggatttgt agccggtaat atggcaagtg agtggcaaga
    23101 agatgaactt caagcaatga taactgaatt accacaacag cgattggttt taccgagtga
    23161 ggttgctcat acatgcgcct atttatatca cccaaatgct agaagtgtta ctggaactat
    23221 acagaaagtt aatggtgctt ggtatattta aaatatagaa ctatttttat gagcaaatgt
    23281 ttttatgatt gatgttataa tatgaatatt acaatgaatc ataatttgtt aattaaacaa
    23341 ggggtgaggt tcaccatgac agttgcagag aaaaaagaat ggtacctaga atacgaaatt
    23401 gaaattaata gaccgggtct tttaggtgat gtatctagtt tattaggtat gttaggtata
    23461 agtattgtta caattaatgg tgttgatcaa ggtaaacgag gccttttaat taaaacagac
    23521 aatcttgaaa aagttgaacg ttttgagcaa atagctcgtg gtataaatga aattgaaata
    23581 acaaagctta aaaaaccaga attaagagac cgtcttgcag taagacatgg aagatatatt
    23641 gagcaagatg caaaagataa gaaaaccttt cgatttgagc gtgaagattt aggcttgtta
    23701 gtagactttt tagctgaatt gttcaaagaa gaaggtcata agttgattgg cattagaggt
    23761 atgccacggg ttggtaaaac tgaatcaatt gttgcgggaa gtgtttgtgc acataagaga
    23821 tggttattta ttagttctac tttaataaaa caaactgtac gtagctctct aattaaaggg
    23881 gaatatgatg ccaatcatgt atacattatt gatggtgcag ttactgccag agaatctaat
    23941 ccaaaacatc aagagcttgt taacgaagtt atgacgttac catcaatcaa agtcgttgaa
    24001 catccagatt tatttgttga aacaagtact tgtacaatgg aagattttga ctatattatc
    24061 gaattgagag aaaatgaaaa tcaagaaata cattacgaag aaatgaagaa acagacagtc
    24121 caaagtaaga ataatttaga ttttggagat ccgtttggtg gtggttttgg tttcttcgag
    24181 taagtgtaag gaggctatga attgaaaacg gtcggtgaag cgctaaaagg tagacgtgaa
    24241 aggttaggaa tgactttaac agaattagag caacgtactg gaattaaacg tgaaatgcta
    24301 gtgcatattg aaaataatga attcgatcaa ctaccgaata aaaattacag cgaaggattt
    24361 attagaaaat atgcaagcgt agtaaatatt gaacctaacc aattaattca agctcatcaa
    24421 gatgaaattc catcgaacca agccgaatgg gacgaagtaa ttacagtttt caataataat
    24481 aaagacttag attataagag taaatcaaaa gagccaatac aattattagt aatcatgggt
    24541 attacagttt taataacttt attgttatgg atcatgttag tttttatatt ttaacagaaa
    24601 taaattagtg agaaatgagg gtgtataatg aatattccga accagattac ggtttttaga
    24661 gtagtgttaa taccagtttt tatattgttt gcgttagttg attttggatt tggcaatgtg
    24721 tcatttctag gaggatatga aataagaatt gagttattaa tcagtggttt tatttttata
    24781 ttggcttccc ttagcgattt tgttgatggt tatttagcta gaaaatggaa tttagttaca
    24841 aatatgggga aatttttgga tccattagcg gataaattat tagttgcaag tgctttaatt
    24901 gtacttgtgc aactaggact aacaaattct gtagtagcaa tcattattat tgccagagaa
    24961 tttgccgtaa ctggtttacg tttactacaa attgaacaag gatttgtaag tgcagctggt
    25021 caattaggta aaattaaaac agcagttact atggtagcaa ttacttggtt gttattaggt
    25081 gatccattgg caacattgat tggtttgtca ttaggacaaa ttttattata cattggcgtt
    25141 atttttacta tcttatctgg ctttgaatac ttttataaag gtagagatgt ttttaaacaa
    25201 aaataaatat ttgtttatac tagatttcat tttcatatgg aatctagttt ttttaatccc
    25261 aattttagaa attagccacg caattgttta taatgatata ttgtaaaaca atatttgttc
    25321 atttttttag ggaaaatctg ttgtagcatc tgatacattg aatctaaaat tgatgtgaat
    25381 ttttaaatga aatacatgaa aaaatgaatt aaacgataca agggggatat aaatgtcaat
    25441 tgccattatt gctgtaggct cagaactatt gctaggtcaa atcgctaata ccaacggaca
    25501 atttctatct aaagtattta atgaaattgg acaaaatgta ttagaacata aagttattgg
    25561 agataataaa aaacgtttag aatcaagtgt acgtcatgcg ctagaaaaat atgatactgt
    25621 tattttaaca ggtggcttag gtcctacgaa agatgactta acgaagcata cagtggccca
    25681 gattgttggt aaagatttag ttattgatga gccttcttta aaatatattg aaagctattt
    25741 tgaggaacaa ggacaagaaa tgacacctaa taataaacaa caggctttag taattgaagg
    25801 ttcaactgta ttggcaaatc atcatggcat ggctccagga atgatggtga attttgaaaa
    25861 caaacaaatt attttattac caggtccacc gaaagaaatg caaccaatgg tgaaaaatga
    25921 attgttgtca cattttataa accataatcg aattatacat tctgaactat taagatttgc
    25981 gggaataggt gaatctaaag tagaaacaat attaatagat cttatcgata aacagactaa
    26041 tcctacgatt gcgcctttgg cgggaagtca tgaagtatat attagattga ctgcaaatgc
    26101 cgactcaaaa gaacaagcac aatcattgat tcaacctgtt aaacaagaaa ttcttgatcg
    26161 tattggagaa tattattatg gttcagatga cacattaatt gagcaagctg taataaagaa
    26221 aattcatgaa ccttttgtaa tatatgatgg tattactaat ggtgctttat atcatcgatt
    26281 gaaagaagtg gatttaaacg atgttctaaa gggtatgatt aatcacaatg aaaactttgt
    26341 tgatattaat aaacctattg agcagcaatt aaaagatgca gtgcaatttg ttaataaatt
    26401 gtttaatgtg tcatcagcaa ttattctatt agagtatgat ggtgtagtcc atataggcta
    26461 tgataataac tttgaattta aaactgagca atttaaaatg tctaaatcta gaaatttatt
    26521 aaagaacaga agtcaaaatt atgcgctcat aagattatta aattggctta gaacaacaaa
    26581 ttaattgtat tatcgataaa aatataagca cgtttgttcg tttttgcgtt ttgatttcaa
    26641 gattttatac gaacaaatat tcgcaaaaca cttgtatttt attttgaatc cttgtatagt
    26701 attggtaaga taattaaaag atagcaattt caattaggag gtctcgcttt ggataacgat
    26761 cgtcaaaaag ctttagatac agtaattaaa aatatggaga aatctttcgg taaaggtgcc
    26821 gtaatgaagt tgggtgacaa tataggtcgc cgagtttcaa ctacatcaac tggttcagtt
    26881 acattagata atgcgctagg tgtaggtggc tatcctaaag gacgaattat tgaaatttat
    26941 ggtcctgaaa gttctggtaa gacaacagta gcgcttcacg ctattgctga agtacaaagt
    27001 aatggcgggg tggcagcatt tatcgatgct gaacatgctt tagatccaga atatgctcaa
    27061 gcattaggtg tagatatcga taatttatat ttatcgcaac cggatcatgg tgaacaaggt
    27121 cttgaaatcg ccgaagcatt tgttagaagt ggtgcagttg atattgtagt tgtagactca
    27181 gttgctgctt taacacctaa agctgaaatt gaaggagaaa tgggagacac tcacgttggt
    27241 ttacaagctc gtttaatgtc acaagcgtta cgtaaacttt caggtgctat ttctaaatca
    27301 aatacaactg ctattttcat caaccaaatt cgtgaaaaag ttggtgttat gttcggtaat
    27361 ccagagacta caccaggtgg acgtgcatta aaattctata gttcagtaag actagaagta
    27421 cgtcgtgcag aacagcttaa acaaggacaa gaaattgtag gtaatagaac taaaattaaa
    27481 gtcgttaaaa ataaagtggc accaccattt agagtagctg aagttgatat tatgtatgga
    27541 caaggtattt ctaaagaggg tgaacttatt gatttaggtg ttgaaaacga catcgttgat
    27601 aaatcaggag catggtattc ttacaatggc gaacgaatgg gtcaaggtaa ggaaaatgtt
    27661 aaaatgtact tgaaagaaaa tccacaaatt aaagaagaaa ttgatcgtaa attgagagaa
    27721 aaattaggta tatctgatgg tgatgttgaa gaaacagaag atgcaccaaa gtcattattt
    27781 gacgaagaat agtacacaaa tttatatcta tagttaaact tagcaaatat ccttatagga
    27841 ttgattgaaa gtgatattca tctcataaag ctagaataat
//
LOCUS       DAHHYJ010000017        26216 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000017 DAHHYJ010000000
VERSION     DAHHYJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.017"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            68..556
                     /locus_tag="NBK22_000568"
     CDS             68..556
                     /locus_tag="NBK22_000568"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD2413774.1"
                     /translation="MAIKKLVPASHPILTKKAQAVKTFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            549..1484
                     /locus_tag="NBK22_000569"
     CDS             549..1484
                     /locus_tag="NBK22_000569"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCD2413775.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPNLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDTQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAGKKRMLAANYLSGAQNALVGKKLI"
     gene            1481..2788
                     /gene="rsmB"
                     /locus_tag="NBK22_000570"
     CDS             1481..2788
                     /gene="rsmB"
                     /locus_tag="NBK22_000570"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCD2413776.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            2791..3885
                     /gene="rlmN"
                     /locus_tag="NBK22_000571"
     CDS             2791..3885
                     /gene="rlmN"
                     /locus_tag="NBK22_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCD2413777.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     DFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            3892..4635
                     /locus_tag="NBK22_000572"
     CDS             3892..4635
                     /locus_tag="NBK22_000572"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCD2413778.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GDQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            4632..6626
                     /gene="pknB"
                     /locus_tag="NBK22_000573"
     CDS             4632..6626
                     /gene="pknB"
                     /locus_tag="NBK22_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCD2413779.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDMILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKGYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGEKSESSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDDV"
     gene            6854..7729
                     /gene="rsgA"
                     /locus_tag="NBK22_000574"
     CDS             6854..7729
                     /gene="rsgA"
                     /locus_tag="NBK22_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCD2413780.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFEVQNINEGYIHQVFERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLNARVLVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            7730..8374
                     /locus_tag="NBK22_000575"
     CDS             7730..8374
                     /locus_tag="NBK22_000575"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCD2413781.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            8381..9022
                     /locus_tag="NBK22_000576"
     CDS             8381..9022
                     /locus_tag="NBK22_000576"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCD2413782.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(9403..9591)
                     /locus_tag="NBK22_000577"
     CDS             complement(9403..9591)
                     /locus_tag="NBK22_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCD2413783.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            10034..10408
                     /locus_tag="NBK22_000578"
     CDS             10034..10408
                     /locus_tag="NBK22_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD2413784.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            10423..12069
                     /gene="fakA"
                     /locus_tag="NBK22_000579"
     CDS             10423..12069
                     /gene="fakA"
                     /locus_tag="NBK22_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCD2413785.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAIEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEYPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQAVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            12259..14319
                     /gene="recG"
                     /locus_tag="NBK22_000580"
     CDS             12259..14319
                     /gene="recG"
                     /locus_tag="NBK22_000580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCD2413786.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEVLQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDIDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            14522..15094
                     /gene="fapR"
                     /locus_tag="NBK22_000581"
     CDS             14522..15094
                     /gene="fapR"
                     /locus_tag="NBK22_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCD2413787.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            15099..16085
                     /gene="plsX"
                     /locus_tag="NBK22_000582"
     CDS             15099..16085
                     /gene="plsX"
                     /locus_tag="NBK22_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCD2413788.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNQDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            16078..17004
                     /gene="fabD"
                     /locus_tag="NBK22_000583"
     CDS             16078..17004
                     /gene="fabD"
                     /locus_tag="NBK22_000583"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCD2413789.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAAKTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSSLLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            16997..17731
                     /gene="fabG"
                     /locus_tag="NBK22_000584"
     CDS             16997..17731
                     /gene="fabG"
                     /locus_tag="NBK22_000584"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCD2413790.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            18102..18335
                     /locus_tag="NBK22_000585"
     CDS             18102..18335
                     /locus_tag="NBK22_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD2413791.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            18451..19182
                     /locus_tag="NBK22_000586"
     CDS             18451..19182
                     /locus_tag="NBK22_000586"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCD2413792.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            19329..22895
                     /gene="smc"
                     /locus_tag="NBK22_000587"
     CDS             19329..22895
                     /gene="smc"
                     /locus_tag="NBK22_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCD2413793.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLIELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRFT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            22895..24145
                     /gene="ftsY"
                     /locus_tag="NBK22_000588"
     CDS             22895..24145
                     /gene="ftsY"
                     /locus_tag="NBK22_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCD2413794.1"
                     /translation="MSFFKRLKDKFATNEENEEVKSLTEEQGQDKLEDTHSEGSTQDA
                     NDLAENAEVKKKSRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGSK"
     gene            24132..24464
                     /locus_tag="NBK22_000589"
     CDS             24132..24464
                     /locus_tag="NBK22_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCD2413795.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            24490..25857
                     /gene="ffh"
                     /locus_tag="NBK22_000590"
     CDS             24490..25857
                     /gene="ffh"
                     /locus_tag="NBK22_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCD2413796.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTQLMGGENTSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
BASE COUNT         9800 a         3490 c         5163 g         7763 t
ORIGIN      
        1 gtaaggtcgc atttttaata tatttacgtt ataatagttt gatgaatttt attgaggagt
       61 aagagttatg gcgattaaaa agttagtacc agcatcgcat cctattttaa cgaaaaaagc
      121 gcaagcagtt aaaacatttg atgattcgtt aaaaagatta ttacaagatt tagaagatac
      181 aatgtatgca caagaagctg ctggcttatg tgcacctcaa attaatcagt cattgcaagt
      241 ggcaattatt gatatggaaa tggaaggatt attacaactt gttaatccga aaattattag
      301 tcaatcaaat gaaacaataa cagacttaga aggttcaatt acattgccag atgtttacgg
      361 cgaagtgaca agaagtaaaa tgatagttgt cgaaagttat gacgtcaatg ggaacaaagt
      421 tgaactaact gcacatgaag atgtagcaag aatgattttg catattatag atcaaatgaa
      481 cggtatccct tttacagaac gtgcggaccg tattttaaca gataaagaag tggaggcata
      541 ttttataaat gactaaaata atatttatgg gtacaccaga cttttcaaca actgttttag
      601 aaatgcttat tgcagaacat gatgtcattg cagtcgtaac gcaaccagat cgacctgttg
      661 gacgtaaacg tgttatgaca ccaccaccag ttaaaaaagt tgcaatgaaa tatgatttac
      721 ctgtatatca acctgaaaaa ttaagtggat cagaagaatt agaacaattg cttcaattag
      781 atgtagattt aattgtaact gctgcttttg gacaattatt acctgaatca ttgttggcat
      841 taccaaatct tggggcaatt aatgtacatg catcattgtt accgaagtat agaggtggtg
      901 caccaattca tcaggcaatt atcgatggtg aacaagaaac cggcataaca attatgtata
      961 tggttaaaaa attagatgcg ggtaatatta tttcgcaaca agcaattaaa atagaagaaa
     1021 atgataatgt cggtacgatg catgataaat taagtgtatt aggggcagat ttattaaaag
     1081 aaactttacc atctattata gagggcacaa atgaaagcgt acctcaagat gatacgcaag
     1141 caacatttgc ttccaatatt cgacgcgaag atgagcgaat tagctggaat aaaccaggaa
     1201 gacaagtgtt taatcaaatt cgtggattat caccatggcc agttgcttat acaactatgg
     1261 atgacactaa cttgaaaata tacgatgctg aactcgttga gactaataag ataaacgagc
     1321 ctggaaccat tatagaaacg actaaaaaag ccattattgt tgctacaaat gataatgaag
     1381 ctgttgcaat taaagatatg caattagctg ggaaaaagag aatgttagct gccaattatt
     1441 taagtggtgc gcaaaacgca ctagtaggga agaaacttat atgatagaaa acgtgagaag
     1501 tcttgctttt gacacgattc aagatatatt aaatgaaggt gcgtatagta acttgcgtat
     1561 caatgaagtg ttgtcagaaa atgaattaaa tgcaatggat aaggctttat ttacagaaat
     1621 tgtctacgga accgttaaaa gaaaatatac gttagatttt tatttaaagc cttttgtgaa
     1681 aacaaagatt aaggcatggg ttaggcaatt attatggatg agtatttatc aatatgttta
     1741 tttagataaa gttccaaatc atgccattat taatgaagca gttgaaatag caaaagaacg
     1801 cggtggctat cataatggta atgtcgtaaa tggtatttta cgtacaatga tgcgtagtga
     1861 cttacctgat tttaatgaaa ttgcagatcc taaaaaaaga atggcaatcg agtatagtat
     1921 gccgaagtgg attatagatc attgggcaac acattatggt ctcgaagaaa ctgaaacaat
     1981 tttacagtca tttttagaaa cgacatcaac aactgtgcgt gccaacctga cgcgagcatc
     2041 attagatgat attattgaaa agttgcaaga cgaaggttat gacgttgaaa aagatcatga
     2101 cttaccttat tgtctccata taggaggaca accaattatt cattctcgtt catttaaaga
     2161 tggattcgtt tcaattcaag ataaaagctc aatgtttgtt gcacacatta tgaatgtaga
     2221 ccgacatgat cacgtattag atgcatgtag tgcacctggc ggtaaagctt gtcacattgc
     2281 tgaagtttta atgccagaag ggcaagttga cgcttcagat atacatgatc acaaaataga
     2341 cttaattaat tttaatataa aaaaattacg attaacaaat attaaagctt ttcaacatga
     2401 tgcgacaaaa ccttatgata aaacatacga taagatactt gttgatgcac catgtagcgg
     2461 attaggtgta atgagacata agccggagat taagtatact caaagcaaac aacatattga
     2521 gtcactagtt gaattacagc ttgaaatatt ggaaaatgta aaaaacaatg taaaaatagg
     2581 tggcgaaatc atctattcaa catgtacaat tgagcaacta gaaaatgaaa acgtgattta
     2641 tacgtttttg aaaaataata aaaacttcga atttgaaccg tttcaacatc cgataactgg
     2701 agagttggtc aaaacgttac aaatcatgcc gcaagacttt aattcagatg gattctttat
     2761 cactaagata aaaagaaagg acaattagga atgataactg ctgaaaagaa aaagaagaat
     2821 aaatttcttc caaattttga caagcaatca atatattcat tgcgatttga cgaaatgcaa
     2881 aactggctcg ttgaacaagg gcaacaaaaa tttcgagcga aacagatttt tgaatggtta
     2941 tatcaaaaaa gagtagattc gattgatgaa atgacgaact tatcgaaaga cttacgacag
     3001 cttttaaaag ataactttac tgttacaact ttaacaactg tagtaaaaca agaaagtaaa
     3061 gacggtacaa ttaaattctt atttgaatta caagatggct atacaattga aactgtttta
     3121 atgagacatg attatggaaa ttcagtatgt gtaacgacac aagtaggttg tcgcatcgga
     3181 tgtacgtttt gtgcttctac acttggcggc ttaaaaagaa accttgaagc tggcgaaatt
     3241 gtttcacaag ttttaacagt tcaaaaagcc cttgatgcta cagaagagcg cgtatctcaa
     3301 attgtcataa tgggtatcgg tgaaccattt gaaaattatg atgaaatgat ggacttttta
     3361 agaatcgtca atgatgataa cagtttaaat attggtgcac gtcacattac agtatcaaca
     3421 tcaggtatca ttcctagaat atatgacttt gcggatgaag atattcaaat taattttgct
     3481 gtaagcttac acgccgcaaa agatgaagtg cgatcacgct tgatgccaat taaccgtgca
     3541 tataatgttg agaagttaat cgaagcaatt caatattatc aagaaaaaac aaatcgtcgt
     3601 gttacttttg aatatggtct gtttggtggt gtgaatgacc aactagaaca tgcaagagaa
     3661 ttagcacatt taataaaagg cttaaactgc catgttaact taattcctgt caaccatgtt
     3721 ccagaaagaa attatgtgaa aacggctaaa aatgatatct ttaaatttga aaaagaatta
     3781 aagagactag gaattaatgc cacaatacgt cgtgaacaag gttcggatat tgacgcagct
     3841 tgtggtcaat taagagcaaa ggaacgacaa gtagaaacga ggtaaagaca aatgctagag
     3901 gcacaatttt ttactgatac tggacaacat agagataaga atgaagatgc gggtggtatt
     3961 ttttataatc aaactaatca acaactttta gttctgtgtg atggtatggg tggccataaa
     4021 gcaggagaag ttgcaagtaa atttgttaca gatgagttga aatcccgttt tgaagcggaa
     4081 aatcttatag aacaacatca agctgaaaat tggttgcgta ataatataaa agatataaat
     4141 tttcagttat atcactatgc acaagaaaat gcagaatata aaggtatggg tacaacatgt
     4201 gtttgtgcac ttgtttttga aaaatcagtt gtgatagcaa atgtcggtga ttctagagcc
     4261 tatgttatta atagtcgaca aattgaacaa attactagtg atcactcatt tgttaatcat
     4321 cttgttttaa cgggtcaaat tacgccggaa gaagcattta cacatccaca acgtaatatt
     4381 attacgaagg tgatgggcac agataaacgt gtgagtccag atttgtttat taagcgatta
     4441 aatttttatg attatttatt attaaattca gatggattaa ctgattatgt taaagacaat
     4501 gaaattaagc gtttgttagt aaaagaaggt acaatagagg atcatggtga tcaattaatg
     4561 caattggcat tagataacca ttcgaaagat aacgttactt tcatactcgc ggctattgaa
     4621 ggtgataaag tatgataggt aaaataataa atgaacgata taaaattgta gataagcttg
     4681 gcggcggtgg catgagtacc gtttatcttg ctgaagatat gatacttaac attaaagttg
     4741 caattaaggc gatttttata ccacctagag aaaaagaaga aacattaaaa cgttttgaac
     4801 gagaagtaca taactcatca cagctatcac atcaaaatat agtaagtatg atcgatgttg
     4861 atgaagaaga tgactgttac tacttagtaa tggaatatat cgaaggtccg actttgtctg
     4921 agtatattga aagtcatggg ccattaagtg ttgacacggc gattaatttt acgaatcaaa
     4981 tattggacgg tattaaacat gcgcatgata tgcgtattgt acatagagat attaagccac
     5041 aaaatatatt aattgacagc aataaaacgt tgaaaatatt tgattttgga attgctaaag
     5101 ctttaagtga gacgtcttta actcagacta atcatgtgtt aggtactgtg cagtactttt
     5161 cgccagaaca agcaaaaggt gaggcaactg atgaatgtac agatatttat tctataggta
     5221 ttgtgttata tgaaatgctt gttggtgaac caccctttaa tggagaaact gcagttagca
     5281 ttgcgattaa acatattcag gattcagtgc caaatgtgac aacagatgta cgtaaggata
     5341 ttccgcaatc tttaagtaat gtcattttac gtgctacaga aaaagataaa gcgaatcgtt
     5401 acaaaacaat tcaagaaatg aaagatgact tgagtagtgt tttacatgaa aatcgagcga
     5461 atgaagatgt ctatgaactc gataaaatga aaacgatagc ggtacctttg aaaaaagaag
     5521 atctagcaaa gcatattagt gaacataagt cgaatcaacc taaacgtgaa acgacgcaag
     5581 tacctattgt aaatgggcct gctcatcatc agcaattcca aaagccagaa ggtacggttt
     5641 acgaaccaaa acctaaaaag aaatcaacac gaaagattgt gctcttatca ctaatctttt
     5701 cgttgttaat gattgcactt gtttcttttg tggcaatggc aatgtttggt aataaatacg
     5761 aagagacacc tgatgtaatc gggaaatctg taaaagaagc agagcaaata ttcaataaaa
     5821 acaacctgaa attgggtaaa atttctagaa gttatagtga taaatatcct gaaaatgaaa
     5881 ttattaagac aactcctaat actggtgaac gtgttgaacg tggtgacagt gttgatgttg
     5941 ttatatcaaa gggccctgaa aaggttaaaa tgccaaatgt cattggttta cctaaggagg
     6001 aagccttgca gaaattaaaa tcgttaggtc ttaaagatgt tacgattgaa aaagtatata
     6061 ataatcaagc gccaaaagga tacattgcaa atcaaagtgt aaccgcaaat actgaaatcg
     6121 ctattcatga ttctaatatt aaactatatg aatctttagg cattaagcaa gtttatgtag
     6181 aagactttga gcataaatcc tttagcaaag ctaaaaaagc cttagaagaa aaaggattta
     6241 aagttgaaag taaggaagag tatagtgacg atattgatga gggtgatgtg atttctcaat
     6301 ctcctaaagg aaaatcagta gatgaggggt caacgatttc atttgttgtt tctaaaggtg
     6361 aaaaaagtga atcatcagat gtcaaaacga caactgaatc ggtagatgta ccatacactg
     6421 gtaaaaatga taagtcacaa aaagttaaag tttatattaa agataaagat aatgacggtt
     6481 caactgaaaa aggtagtttc gatattacta gtgatcaacg tatagacatt cctttaagaa
     6541 ttgaaaaagg aaaaacagca agttatattg ttaaagttga cggtaaaact gtagctgaaa
     6601 aagaagtcag ctatgatgat gtataaatat aattgaagta aatgtaccga ggtttctatt
     6661 tggaagtctc ggtattttta tgttgagatt gcggtagttt taaaatgctt catgtcatca
     6721 tatacaggtg agcattgtct atcaattctg tatcactctc acaatattta tatcatttga
     6781 aatgatcatt tatatattaa ttttccagtc tttgatataa taaatgcagt aaaattaatc
     6841 gagaggtgcc attttgaaga caggtcgaat agtgaaatca attagtgggg tatatcaagt
     6901 agacgttaat ggcgaacgtt tcaatacaaa accacgagga ttatttagaa agaaaaaatt
     6961 ttcaccggta gttggtgata tagtggaatt tgaagtacaa aacattaacg aaggctatat
     7021 tcatcaagtg tttgagcgga aaaatgagtt gaaaagacca cctgtaagta atatagatac
     7081 actagtaatt gtaatgagtg ctgtcgagcc aaatttttca acgcaattat tagatcgatt
     7141 tttagttatt gcacattcgt atcagttaaa tgcgagagtt ttggtgacta aaaaagataa
     7201 aacaccaatt gaaaagcagt tcgaaattaa tgagttgttg aaaatatatg aaaatattgg
     7261 ctatgagact gaatttattg gaaatgatga tgatcgaaaa aaaattgtag aagcttggcc
     7321 agctggactt atagtactta gtggtcaatc aggtgtcggt aagtccactt tcttaaatca
     7381 ttatcgtcca gaacttaatc ttgagacaaa tgatatatca aaatcattaa atcgaggaaa
     7441 gcatactaca agacatgtcg aactattcga acgtcaaaac ggttatattg cagacacacc
     7501 tggattcagt gctttagatt ttgatcatat agataaagat gaaataaaag attattttct
     7561 tgaattaaat cgatatggtg aaacatgtaa gtttaggaat tgtaatcata tcaaagaacc
     7621 taattgtaat gttaagcatc aattagagat agggaatatt gcgcaattta gatacgacca
     7681 ttatttacaa ctatttaatg aaatttcaaa tagaaaggtt agatattaaa tgacaaaact
     7741 atatccatca ttattatctg ttgatttttt ggatttacaa catgaattaa aacgacttga
     7801 agaagcaggt gtcgacggag ttcattttga tgttatggat ggtcaatttg tgcctaatat
     7861 atctattggt ttaccaatat tagatgcagt aagaaaaggc acaacattac ctatagacgt
     7921 acatttgatg attgaaaatc cagaaaagta tattgcatca tttgcagaac atggtgccga
     7981 tatgatttca attcatgtcg aatcaacgcc tcatattcat cgtgctattc aaatgattaa
     8041 acatttagat aaaaaagctg gtgtagtaat taatcctggt acaccaatat cacaaattga
     8101 acctatttta gacattgttg attatgtact agtgatgaca gttaacccag ggtttggtgg
     8161 tcaatcattt attgatcaat gcgtagaaaa aatagcgggt cttaatgcta ttaaaatgga
     8221 acgtcaatta aactttgata ttgaagttga tggaggcgta aataccgata cagcgaaagt
     8281 ttgtgttgaa aatggtgcta caatgctagt aacaggttca tttttcttta aacaagagga
     8341 ttataaaaaa gtcacacaag cattgaaagg ttgagtgtat atgcatataa atttattatg
     8401 ttctgatcga cacttgccgc aagatatttg ggccaaaagt aatgaaggta aatggggcgg
     8461 cgttgataga ggtgctttga ttttattgaa gcatcaaatt atcccttttt tctcagtggg
     8521 agactttgat tcagtcagta aagaagaacg ccaacttcta acagaacagt tacaaatcaa
     8581 accagttcaa gctgaaaaag ctgatacgga tttagcttta gcggttgata aagctgttgc
     8641 acttggattt gatagtatta caatttatgg tgcaacaggc ggacgattag atcacttttt
     8701 tggggcaatt cagttattat tgaaaaaagc atattataaa catgatgttc atatagaagt
     8761 tatcgatcaa caaaataaaa ttgaattatt gcctaaaggt caacatacag ttgaaaaaga
     8821 taagagttat ccgtacattt catttatacc gatgactgat gatgtagaac tttctctagc
     8881 aggttttaaa tataatttag ctagacaaat gcttaatata ggttctactt taactatttc
     8941 aaatgaaatt gagtctttgc aagcgaaagt aactgtacat gatgggttga ttttgcaaat
     9001 tagaagtaca gatttaaatt aagtatattc aaatttgtga ggatttttaa taaagtgcat
     9061 gagttattta acaaagtgac gtttatagat agaatttata acataataat agatgaatca
     9121 cgctgtaatt aatgaagatt atggcgtgat ttattattga aaggaaaatt gtaggtccta
     9181 gtacattgat ttatgtgcca aaatcgatat tgaacccata agtccaattt gacagagaac
     9241 cattattaaa cgtattaacc aaattatatt gatagttcga atttcactat gaaccattat
     9301 gtattgtttg gtaacaaaaa aagaactaag aggttgagct cttagttctt aagtaaatgt
     9361 gtcctccaca taaggtcaca ttttttatgt gcaatatatt tattaaactc tagttacttt
     9421 accagatttt aaagcacgtg cagaaaccca aactttttta ggtttaccgt caactaggat
     9481 tctaactttt tgaaggttag cgttccatct acgtttagta gagtttaaag cgtgtgaacg
     9541 tctgttacca gtcgaagctt tacgacctgt tacgaaacat tgtttaccca tatgagtacc
     9601 tcctttaata aatataaata cacataacta cttatatact taattaagat agcatagttt
     9661 catttgaaaa acaatgaata attttcacat aaaagtcaaa aatactgagt tttgtgatat
     9721 aattgtagac tgtgaagtta tgtagtatga tattttagag aaataagtga atgaaacatt
     9781 taaaattttt atttaatgat actacatcta ttaaaagaaa acgcctatag ataatcgtca
     9841 tgagtgtaaa ttcagtttac tcaaaatata ataggtgatt taagcgtaag tcattctgat
     9901 aagttgtacc attgatattt tataaaaata atcaatgaaa cttaaagcgt ttatgactac
     9961 actaagtggt aaaattttaa aagtgattag ggaaaacgaa taataaactt tgaaatttag
    10021 gagggacaaa gatatgacat tagagatttc aaatgattac ggtaaaattg atatttcaaa
    10081 cgaagtgatt gcttcggttg taggtggaaa ggccgttgaa tgttatggta ttgtaggtat
    10141 ggcatctaga caacaagtta gagatggtat tgcggaaata ctaggacatg aaaattatgc
    10201 taaaggcatc aaagtaactg aaaataatgg cgtagtggat atagatatgt acattattgt
    10261 aagttacggt gtgaaaatat ctgaagttgc caataatgta caatcaacag tgaaatatac
    10321 tttggaaaaa tcacttaatg tatcagtaaa ttcaatcaat atatatgtac aaggtgtacg
    10381 tgtgaataat acaggcaaga aagcttagga ggacaacttg aaatgattag caaaattaat
    10441 ggtaaattat ttgccgatat gattatacaa ggggcacaaa atttatctaa caatgcagat
    10501 ttggtagatt ctttgaatgt gtatccagtg ccagatggtg atacaggaac aaatatgaat
    10561 cttactatga cttcaggtcg cgaagaagta gagaataatt tgtcgaaaaa tatcggcgaa
    10621 ttaggtaaaa cattctcgaa aggtttacta atgggtgcaa gaggtaactc tggtgtcatc
    10681 ttgtcacaat tattcagagg attttgtaaa aatattgaaa gtgaatctga aattaattca
    10741 aaattgttag ctgaaagttt tcaagctggt gttgaaacgg catataaagc tgttatgaaa
    10801 ccagttgaag gtacaatact tacagttgca aaagatgctg cgcaagctgc aatagaaaaa
    10861 gcaaataata ctgaagattg tatagaatta atggagtaca ttattgtaaa agccaatgaa
    10921 tcacttgaaa acacaccaaa cttattagct gtacttaaag aagttggtgt tgttgatagt
    10981 ggcggtaaag gtttgttatg cgtttacgaa ggattcttaa aagcgcttaa aggtgaaaaa
    11041 gttgaagcca aagttgcaaa gatagataaa gatgaatttg tacatgatga acatgatttc
    11101 catggtgtaa ttaatactga agatattatt tatggctatt gtactgaaat gatggttcgt
    11161 tttggaaaga ataaaaaagc ctttgatgaa caagaattca ggcaagatat gagtcaattt
    11221 ggtgattctt tattagtcat taatgatgaa gaaattgtga aagttcacgt gcataccgaa
    11281 tacccaggta aagtgtttaa ttatggtcaa caatatggtg aattaattaa acttaaggtt
    11341 gaaaatatga gagaacagca tcgtgaagtg attcgaaaag aacagcacac agctaaaccg
    11401 aaaatggaaa cggttgaaac agcgattatt actatttcta tgggtgaagg tatttcagag
    11461 atatttaaat caatgggtgc cacacatatc attagtggtg gacaaacgat gaatccttct
    11521 acagaagata tcgttaaagt cattgaacaa tcaaaatgta aacgtgcaat tattttaccg
    11581 aataataaaa atattttaat ggcaagtgaa caagcagcga gtattgttga tgcagaagct
    11641 gttgttattc caacgaaatc tattcctcaa ggtataagcg cactattcca atatgatgtg
    11701 gacgcaacac ttgaagaaaa taaagcgcaa atggctgatt cagtaaataa cgttaaatct
    11761 ggttcattaa cgtacgctgt tcgtgatacg aaaattgatg gcgttgagat taaaaaagac
    11821 gcgtttatgg gcttgattga agataagatt gtaagcagcc aaagtgatca attaacaacg
    11881 gttactgagt tgttaaatga gatgttagca gaagatagtg aaatattgac tgtgattatt
    11941 ggtcaagatg cagagcaagc agttacagat aacatgataa actggattga agagcaatat
    12001 ccagatgtag aagtggaagt tcatgaaggt ggacaaccaa tttatcaata tttcttttca
    12061 gtagaataaa aatttaaaat aaaaaactac caatgataaa tcatcagttg gtagtttttt
    12121 attttgctat tttagtgaga tagcgggtta aaagtatcat tctcgagttg ctaacaatgt
    12181 catgttcaac ttagtcatga taaaataaat aacatactaa atgatacgta aaatcaaata
    12241 aaacataggt gatttatttt ggctaaagta aacttaatag aaagtccata ttctctttta
    12301 caattaaaag gtataggtcc taagaaaata gaagtattgc aacaactaaa tattcataca
    12361 gtggaagatc ttgttcttta tttgccaact agatatgaag ataatacagt gattgatttg
    12421 aatcaagcag aagatcaatc taacgttacg atagaaggac aagtatatac agctccagta
    12481 gttgcatttt ttggaagaaa taaatcaaaa ttaaccgttc atttaatggt aaataatatt
    12541 gctgtcaaat gtattttttt caatcaaccg tatttaaaaa agaaaatcga attaaatcaa
    12601 actataactg ttaaaggtaa gtggaatagg gttaaacagg aaattactgg taatagggtt
    12661 ttctttaatt cacaagggac acaaactcaa gaaaacgcag atgttcaatt agaaccagtc
    12721 tatcgtatta aggaaggtat taaacaaaag caaatacgag accaaattag acaagcgtta
    12781 aatgatgtga caattcatga atggttaact gatgaactaa gagaaaaata taaattagag
    12841 accttggact ttactttgaa cacattacat catcctaaaa gtaaagagga tttattacgt
    12901 gctcgtagaa cctatgcatt tactgaactg tttttattcg aattacgtat gcaatggcta
    12961 aatagattag aaaagtcatc tgacgaagca attgaaattg attatgacat agaccaagtt
    13021 aaatcattta ttgatcgttt accttttgaa ctaactgaag cacagaaatc cagtgttaat
    13081 gaaattttta gagatttaaa agcaccaata cgtatgcatc gattacttca aggtgatgta
    13141 ggttcaggaa aaacagtagt tgctgcaatt tgtatgtatg cgttaaaaac ggctggttat
    13201 caatcagcat tgatggtacc aactgaaatt ttagcagagc aacatgctga aagtttaatg
    13261 gctttatttg gagattctat gaacgttgca ttgttaactg ggtcagtaaa aggtaagaaa
    13321 cgaaagatac ttttagaaca acttgaaaat ggtacgattg attgtttaat tggaacccat
    13381 gctttgattc aagatgatgt gattttccat aatgttggtt tagtaattac agatgaacaa
    13441 catcgatttg gtgtgaatca acgccagctt ttaagagaaa aaggtgcaat gacgaatgtg
    13501 ttatttatga cagcaacgcc gataccaaga acactagcaa tatcagtttt tggtgagatg
    13561 gatgtgtctt caattaaaca attaccaaaa ggtcgtaaac ctatcattac tacttgggca
    13621 aagcatgagc aatacgataa agttttgatg caaatgacct cagagttgaa aaaaggtcgt
    13681 caagcatatg tcatttgccc gcttatagaa agttctgagc atctcgaaga cgttcaaaat
    13741 gttgtcgcat tgtacgagtc tttacaacag tattatggtg tttcccgtgt agggttattg
    13801 catggtaagt tgtctgccga tgaaaaagat gaggtcatgc aaaagtttag caatcatgag
    13861 atagatgttt tagtttctac tactgttgtt gaagtaggtg ttaatgtacc gaatgcaact
    13921 tttatgatga tttatgatgc ggatcgcttt ggattatcaa ctttacatca gttacgcggt
    13981 cgtgtaggta gaagtgacca gcaaagttac tgtgttttaa ttgcatcccc taaaacagaa
    14041 acaggaattg aaagaatgac aattatgaca caaacaacgg atggatttga attgagtgaa
    14101 cgagacttag aaatgcgtgg tcctggagat ttctttggtg ttaaacaaag tggattgcca
    14161 gatttcttag ttgccaattt agttgaagat tatcgtatgt tagaagttgc tcgtgatgaa
    14221 gcagctgaac ttattcaatc tggcgtattc tttgaaaata cgtatcaaca tttacgtcat
    14281 tttgttgaag aaaatttatt acatcgtagt tttgactaat tgccatgctg atttgtcaat
    14341 ttgagtgcaa cacttcgtta attgagtgat atgacacttg aactatttaa atgtaaagtg
    14401 gtattttaac aatttataaa ttttcgacta aataatagct aaatattaca gttatttgtt
    14461 gagtcggtta aatagaaagt gttatgatat gtgaggaatg tttaagacta ggtactaaaa
    14521 aatgaggggt gagacgttga aactaaagaa agataaacgt agagaagcaa tcagacaaca
    14581 aattgatagc aatcccttca tcacagacca tgaactaagc gacttatttc aagtgagtat
    14641 acaaacaatt cgtttagatc gcacttattt aaacatacca gaattaagga agcgtattaa
    14701 attagttgct gaaaagaatt atgaccaaat aagttctatt gaagaacaag aatttattgg
    14761 tgatttgatt caagtcaatc caaatgttaa agcgcaatca attttagata ttacatcgga
    14821 ttctgttttt cataaaactg gaattgcgcg tggtcatgtg ctgtttgctc aggcaaattc
    14881 gttatgtgtt gcgctaatta agcaaccaac agttttaact catgagagta gcattcaatt
    14941 tattgaaaaa gtaaaattaa atgatacggt aagagcagaa gcacgagttg taaatcaaac
    15001 tgcaaaacat tattacgtcg aagtaaagtc atatgttaaa catacattag ttttcaaagg
    15061 aaattttaaa atgttttatg ataagcgagg ataaaattat ggttaaatta gcaattgata
    15121 tgatgggtgg cgacaatgcg cctgatatcg tattagaagc cgtacaaaag gctgttgaag
    15181 actttaaaga tctagaaatt atacttttcg gtgacgaaaa aaagtataat ctgaaccatg
    15241 aacgaatcga atttagacat tgttctgaaa agattgaaat ggaagatgag cctgttagag
    15301 cgattaaacg taaaaaagat agctcaatgg taaaaatggc tgaagctgtg aaatctggtg
    15361 aagcagatgg atgtgtgtca gcaggtaata ctggtgcttt aatgtcagct ggtttattca
    15421 ttgttggacg tattaaaggt gtagctagac cggctttagt agtaacattg ccaacgattg
    15481 atggaaaagg ttttgtcttt ttagacgttg gtgcaaatgc tgatgctaaa cctgaacact
    15541 tattacagta tgcgcaacta ggggatattt atgctcaaaa aatcagaggt attgataatc
    15601 cgaaaatctc attattaaat ataggtaccg agccagctaa aggtaatagt ttaacgaaaa
    15661 aatcatatga gttattaaat caagatcatt cattgaattt tgttgggaat attgaagcga
    15721 agacattaat ggatggcgat acagatgttg tagttaccga tggctatact gggaacatgg
    15781 tccttaaaaa tttagaaggt actgcaaaat caatcggtaa aatgttaaaa gatacgatta
    15841 tgagtagtac taaaaataaa ttagcaggtg caatattgaa gaaagattta gcagaattcg
    15901 ctaaaaagat ggattactca gaatacggtg gttccgtatt attaggattg gaaggtactg
    15961 tagttaaagc acacggtagt tcaaatgcta aagcttttta ttctgcaatt agacaagcga
    16021 aaatcgcagg agaacaaaat attgtacaaa caatgaaaga gactgtaggt gaatcaaatg
    16081 agtaaaacag caattatttt tccgggacaa ggtgcccaaa aagttggtat ggcacaagat
    16141 ttgtttaata acaatgatca agcaactgaa attttaactt cagcagcaaa gacgttagac
    16201 tttgatattt tagagacaat gtttactgat gaagaaggta aattgggtga aactgaaaac
    16261 acgcaaccag ctttattgac gcatagttcg tcattattag cagcgctaaa aaatttgaat
    16321 cctgatttca ctatggggca tagtttaggt gaatattcaa gtttagttgc agctgacgta
    16381 ttatcatttg aagatgcagt taaaattgtt agaaaacgtg gtcaattaat ggcgcaagca
    16441 tttcctactg gtgtaggaag catggctgca gtattgggat tagattttga taaagtcgat
    16501 gaaatttgta agtcattatc atctgatgac aaaataattg aaccagcaaa cattaattgc
    16561 ccaggtcaaa ttgttgtttc aggtcacaaa gctttaattg atgagctagt agaaaaaggt
    16621 aaatcattag gtgcaaaacg tgtcatgcct ttagcagtat ctggaccatt ccattcatcg
    16681 ctaatgaaag tgattgaaga agatttttca agttacatta atcaatttga atggcgtgat
    16741 gctaagtttc ctgtagttca aaatgtaaat gcgcaaggtg aaactgacaa agaagtaatt
    16801 aaatctaata tggtcaagca attatattca ccagtacaat tcattaactc aacagaatgg
    16861 ctaatagacc aaggtgttga tcattttatt gaaattggtc ctggaaaagt tttatctggc
    16921 ttaattaaaa aaataaatag agatgttaag ttaacatcaa ttcaaacttt agaagatgtg
    16981 aaaggatgga atgaaaatga ctaagagtgc tttagtaaca ggtgcatcaa gaggaattgg
    17041 acgtagtatt gcgttacaat tagcagaaga aggatataat gtagcagtaa actatgcagg
    17101 cagcaaagag aaagctgaag cagtagtcga agaaatcaaa gctaaaggtg ttgacagttt
    17161 tgcgattcaa gcaaatgttg ccgatgctga tgaagttaaa gcaatgatta aagaagtagt
    17221 tagccaattt ggttctttag atgttttagt aaataatgca ggtattactc gcgataattt
    17281 attaatgcgt atgaaagaac aagagtggga tgatgttatt gacacaaact taaaaggtgt
    17341 atttaactgt atccaaaaag caacaccaca aatgttaaga caacgtagtg gtgctatcat
    17401 caatttatca agtgttgttg gagcagtagg taatccggga caagcaaact atgttgcaac
    17461 aaaagcaggt gttattggtt taactaaatc tgcggcgcgt gaattagcat ctcgtggtat
    17521 cactgtaaat gcagttgcac ctggttttat tgtttctgat atgacagatg ctttaagtga
    17581 tgagcttaaa gaacaaatgt tgactcaaat tccgttagca cgttttggtc aagacacaga
    17641 tattgctaat acagtagcgt tcttagcatc agacaaagca aaatatatta caggtcaaac
    17701 aatccatgta aatggtggaa tgtacatgta atatatttga gctaaagctc attgacgcag
    17761 tggttgactg gtcatccaat ggagaattgt ctgacctagt caactttgcg ggggaaattc
    17821 taagcaacct agataaggtt ccagaatttc tccctaagaa acactaatca ataaattgat
    17881 aagaagatta gaggaacgct tgagctaaag ctcaagataa agacgcagaa atcaataaat
    17941 tgataactgg gatagaggta cgcctgagct aaagctcatg cataagaaac actaatcaat
    18001 aaattgataa gtgtttctaa aatttctact tgttttttag aatttaaaat gggaaaatat
    18061 aatagtctat gtataggcat ttttaaagga ggtgaatcga cgtggaaaat ttcgataaag
    18121 taaaagatat catcgttgac cgtttaggtg tagacgctga taaagtaact gaagatgcat
    18181 ctttcaaaga tgatttaggc gctgactcac ttgatatcgc tgaattagta atggaattag
    18241 aagacgagtt tggtactgaa attcctgatg aagaagctga aaaaatcaac actgttggtg
    18301 atgctgttaa atttattaac agtcttgaaa aataataaat cttacatctg ggtcgtcagt
    18361 attgtcgact cagttttttt ctttaattat caatagtttt aacgtaaaat taaagatgat
    18421 tcaagagcaa cacataaagg agataaaata atgtctaaac aaaagaaaag tgagatagtt
    18481 aatcgtttta gaaagcgctt tgatactaaa atgacagagt taggctttac ttatcaaaat
    18541 attgatttat accaacaagc attttcgcat tcgagtttta ttaatgattt taatatgaat
    18601 cgtttagacc ataatgagcg tttagagttt ttgggtgatg cggtattaga attgacggtt
    18661 tcacgatatt tatttgataa acatcccaac ttgccagaag ggaatttaac aaaaatgcgt
    18721 gccactattg tatgtgagcc ctcacttgta atatttgcga ataaaattgg attgaacgaa
    18781 atgattttac ttggtaaagg tgaagagaaa acagggggac gtacaagacc atcattaata
    18841 tcagatgcat tcgaagcatt tattggggca ttgtatttgg atcaaggact agatatagtt
    18901 tggaaatttg ctgagaaagt tattttccca catgtagaac aaaatgagtt attaggcgtg
    18961 gtagatttta aaacacaatt ccaagaatat gtgcaccagc aaaataaagg tgatgtaacc
    19021 tataatttaa taaaagaaga gggaccggca catcatcgtc tattcacttc agaagttatt
    19081 ctgcaagggg aagcaatagc tgaaggtaaa gggaaaacga aaaaagaatc agaacaacgt
    19141 gctgctgaaa gtgcctataa gcaattaaaa caaattaaat agaaatttat atgatacgac
    19201 atataggtgt tcgatttagg atttaaggaa gaatttttaa tattttatgg aacaacttcg
    19261 aaatttaata tacaaatact aaaatatcgt atacatgtgt tctttaaaat tgtgataagg
    19321 agtttaggat ggtttattta aaatcaatag atgccattgg atttaagtct tttgcagatc
    19381 aaaccaatgt tcaattcgat aaaggtgtaa ctgcaattgt tggtccaaat ggaagcggta
    19441 aaagtaatat tacagatgct attaaatggg tgttgggtga acaatcggct aaatcattac
    19501 gtggctcaaa aatggaagat attatcttct caggtgcaga acatcgcaaa gctcaaaatt
    19561 atgctgaagt acagttaaga ttagataatc attctaaaaa gctcagtgtt gatgaaaacg
    19621 aagttattgt aacaagaaga ttgtatcgaa gtggtgaaag tgagtactac ataaataatg
    19681 accgtgcaag attaaaagat attgccgatt tatttttaga ttctggattg ggaaaagaag
    19741 catatagcat tatctcgcaa ggtagagttg atgaaatact aaatgctaaa ccaattgata
    19801 gacgtcaaat tattgaagaa tcggctggtg tacttaaata taaaaaacgt aaagctgaat
    19861 cattaaataa acttgaccaa acagaagata atttaacgag agtagaagac attttatatg
    19921 atttggaagg tcgtgtagaa cctctaaaag aggaggcagc tatagctaaa gaatataaga
    19981 cactttcaca ccaaatgaaa catagtgaca ttgtagttac agtgcacgat attgatcaat
    20041 atacaaatga caatagacaa ttagatcaac gtttaaatga tttacaaggt caacaagcaa
    20101 ataaagaagc tgacaagcaa cgtttaagcc aacaaattca acaatataaa ggtaaacgtc
    20161 atcaacttga taatgatgtt gaatcgctta attatcaatt agtaaaagct acggaagcct
    20221 ttgaaaaata tacgggacaa ttaaatgttt tagaagaacg taagaaaaat caatctgaaa
    20281 caaatgcacg atatgaagaa gaacaagaaa atttaattga gcttttagaa aatatatcaa
    20341 atgagatttc tgaagctcaa gatacttata agtctctgaa aagtaaacaa aaagaactca
    20401 atgctgtcat tcgtgaactt gaagaacaac tatatgtttc agacgaagca catgatgaaa
    20461 aattggaaga aattaaaaac gaatactata cattaatgtc agagcaatca gatgttaaca
    20521 atgatattcg ttttttaaag catactatag aagagaatga ggctaaaaaa tcaagactag
    20581 attctcgatt agttgaagtt tttgagcaat tgaaagatat tcagggtcaa ataaaaacga
    20641 caaaaaaaga atatcaacag accaacaaag aactttctgc tgtagataaa gaaattaaaa
    20701 atatagaaaa agatctcact gatacaaaaa aagcacaaaa tgaatacgaa gagaaattgt
    20761 atcaagcata tcgatatacc gaaaaaatga aaacacgtat tgatagtttg gcaacgcaag
    20821 aggaagaata tacttatttt ttcaatggcg tcaaacatat tttgaaagct aaaaataaag
    20881 aattaaaggg tattcatggt gcagttgcgg aaattattga tgtgccatct aaattaactc
    20941 aggcaattga aacagcatta ggtgcttcat tacaacatgt cattgtagat tcagaaaaag
    21001 atggacgcca ggctattcaa tttttaaaag aacgtaattt aggtcgtgcg acgtttttac
    21061 cattaaatgt tatacagagt agagtggtag cgactgatat taaatctatt gctaaagagg
    21121 caaacggatt tattagtatt gcttcggaag cagttaaagt agcaccagaa tatcaaaata
    21181 ttatcgggaa tttattaggt aatacgatta tcgttgatca tttaaagcat gcaaatgaat
    21241 tggcacgtgc gattaaatat cgaactcgta ttgttacttt ggaaggtgat attgtaaacc
    21301 ctggtggttc tatgactggt ggtggcgctc gtaagtccaa aagtattctg tctcaaaaag
    21361 acgagttgac aacaatgaga caccaattag aagattactt gcgtcaaaca gaatcatttg
    21421 aacaacaatt taaagagttg aagataaaaa gtgatcaatt aagtgaactg tattttgaaa
    21481 aaagtcaaaa gcataataca cttaaagagc aagtgcatca ttttgaaatg gagctcgata
    21541 gatttactac acaagaaaca caaataaaaa atgatcatga agaattcgaa tttgaaaaaa
    21601 atgatggtta tacgagtgac aaaagtcgac aaactttgag tgaaaaagaa actcatctag
    21661 aaagtattaa agcatcttta aaacgactag aagatgaaat tgaacgctac acaaaacttt
    21721 ctaaagaagg taaggaaagc gttactaaaa cacaacaaac cttacatcag aaacaatctg
    21781 atcttgctgt ggttaaagag cgtattaaaa cacaacaaca gacaatagat cgattaaata
    21841 atcaaaatca acaaactaaa catcaattaa aagatgttaa agaaaaaatt gcattcttta
    21901 attcggatga agtgatgggc gaacaagctt ttcaaaatat taaagatcaa attaatggtc
    21961 aacaagaaac gagaacacgc ttatctgatg aattagataa attgaaacaa caacgtattg
    22021 agttgaatga acaaatcgat gcgcaagaag ctaaactaca agtttgtcac caagatattt
    22081 tagctatcga aaatcactac caagatatta aagctgaaca atcaaagcta gatgtattaa
    22141 ttcatcatgc gatagatcat ttaaatgatg aatatcaatt gactgttgaa cgtgcgaaat
    22201 ctgaatatac gagtgatgaa tcgattgacg cattacgtaa aaaagttaag ttaatgaaga
    22261 tgtcgattga tgaactaggt cctgtaaact taaatgcaat tgaacaattt gaagagttaa
    22321 atgaacgtta tacattttta agtgaacaac gtacagatct tcgtaaagct aaagaaacat
    22381 tagagcaaat tataagtgaa atggatcaag aggttactga aagatttaaa gaaactttcc
    22441 atgctattca aggacatttt acagctgtgt tcaaacaatt gtttggtgga ggcgatgcag
    22501 aattgcaatt aactgaagcc gattatttaa cagctggtat tgatattgtg gtacaaccac
    22561 cgggtaaaaa gttgcaacat ttatcgttac tgagtggtgg tgagcgtgca ttaactgcta
    22621 ttgctttact atttgcaatt ttaaaagtaa gatctgcacc ttttgttata ttagatgagg
    22681 ttgaagctgc actagatgaa gcaaatgtta ttagatacgc aaaatattta aatgagttat
    22741 cagacgaaac acaattcatt gttattacac accgtaaagg aacaatggaa tttgcagata
    22801 ggttatacgg tgtaacaatg caagaatcag gtgttactaa acttgtgagt gtgaatttaa
    22861 atacaataga tgatgtgttg aaggaggagc aataatgagc ttttttaaac gcttaaaaga
    22921 taagtttgca acaaatgaag aaaatgaaga agttaaatcc ttaacagaag aacaaggtca
    22981 agacaaatta gaagatacac attctgaagg ttcaacgcag gacgcaaatg atttagcaga
    23041 aaatgctgaa gtgaaaaaga agtcacgcaa gttgagtgaa gcggattttg atgacgatgg
    23101 cttaatatca attgaagatt ttgaagaaat tgaagctcaa aaaatgggtg ctaaatttaa
    23161 agcaggactc gaaaaatctc gtcaaaattt ccaagaacaa ttaaataatt tgatagcgag
    23221 atatcgtaaa gtagatgaag acttttttga agctttagaa gaaatgttaa tcactgcaga
    23281 cgtcggtttt aatacagtga tgacgttaac tgaagaatta cgtatggaag cacaacgacg
    23341 taatattcaa gatactgaag atttgcgtga agtcattgtt gaaaaaatcg tagagattta
    23401 ccatcaagaa gatgataatt cagaagctat gaacttagaa gatggtcgtt taaatgtcat
    23461 tttaatggtt ggtgtgaatg gtgttggtaa aacaacaaca attggaaaat tagcttaccg
    23521 atataaaatg gaaggtaaaa aagtaatgtt agctgcgggc gatactttta gagcgggtgc
    23581 tattgatcaa ttgaaagttt ggggcgaacg tgttggtgta gacgtaatta gccaaagtga
    23641 aggttctgat ccagctgctg ttatgtatga tgcgattaat gccgctaaaa acaaaggtgt
    23701 tgatatttta atttgtgata ccgctggacg tttacaaaat aaaacaaatc taatgcaaga
    23761 attagaaaaa gttaagcgtg taattaatcg agcagtgcca gatgcgcctc atgaagcatt
    23821 actatgttta gatgctacaa ctggtcagaa tgcgttgtca caagctagaa actttaaaga
    23881 agtaacaaat gttacaggta ttgtattaac gaaattagat ggtacagcca aaggtggtat
    23941 cgtattagcc attcgtaatg aattgcacat tccagttaaa tatgtaggtt taggtgagca
    24001 attagatgac ttacaaccat ttaaccctga aagttatgtc tacggcttat tcgctgatat
    24061 gattgaacaa aatgaagaaa taacaacagt tgaaaatgat caaattgtaa cagaagaaaa
    24121 ggacgataat catgggtcaa aatgatttag ttaaaacgtt acgaatgaat tatttgtttg
    24181 atttttatca atccttattg acgaataaac aacgtaatta tttggaatta ttttatcttg
    24241 aagattattc tttaagtgaa atcgcagata cttttaatgt gagtagacaa gcagtttatg
    24301 ataatataag aagaactggc gatttagttg aagattatga aaagaaattg gaattatacc
    24361 agaaatttga gcaacgccga gaaatatatg atgaaatgaa acaacattta agtaatccag
    24421 aacaaataca acgttatatt caacaattag aagacttaga atagatataa attgagggag
    24481 gacatcgata tggcatttga agggttatca gaacgcttgc aagcgacgat gcaaaaaatg
    24541 cgtggtaagg gtaaacttac tgaagctgat ataaagataa tgatgcgtga agtaagatta
    24601 gcgttacttg aggctgacgt aaactttaaa gtggtaaaag aatttattaa aacagtatca
    24661 gaacgcgcat taggttccga tgtaatgcaa tcattaacac cagggcaaca agttattaaa
    24721 atagttcaag atgaattaac gcagttgatg ggtggagaaa atacgtcgat taatatgtca
    24781 aataaaccac ctactgttgt tatgatggtt ggtttacaag gtgctggtaa aacaacaact
    24841 gcaggtaaat tagcattatt gatgcgtaaa aaatacaaca aaaaacctat gttagttgca
    24901 gcagatattt atcgtccagc agcgataaat caattacaaa cagtagggaa acaaattgat
    24961 attcctgtat acagtgaagg agatcaagta aagccacaac aaattgtaac taatgcatta
    25021 aaacatgcta aagaagaaca tttagacttt gtaatcattg atacagcagg tcgattacac
    25081 atcgatgaag cattgatgaa cgaattaaaa gaagtaaaag acattgctaa accaaacgaa
    25141 attatgttag ttgtcgattc aatgacgggt caagatgctg tcaatgttgc agaatctttt
    25201 gacgatcaac ttgatgtcac aggtgttacc ttaactaaat tagatggtga tacacgtggt
    25261 ggtgcagctt tatctattcg ttcggtgaca caaaaaccaa ttaaatttgt tggtatgagt
    25321 gaaaagttag atggtttaga gctattccat cctgaacgta tggcatcacg tattttaggt
    25381 atgggtgatg tgttaagttt aattgaaaaa gcgcaacaag atgtggatca agaaaaagca
    25441 aaagatttag agaaaaagat gcgtgagtca tcgtttactt tagatgattt tttagaacaa
    25501 cttgatcagg tgaaaaatct aggaccactg gatgatatta tgaaaatgat tccaggtatg
    25561 aataaaatga aagggctaga taagcttaat atgagtgaaa agcaaattga tcatattaaa
    25621 gcgattatcc agtcaatgac gccggctgaa agaaacaatc cagacacatt gaatgtatca
    25681 cgtaaaaagc gtattgctaa agggtctggt cgttcattac aagaagtcaa tcgtttgatg
    25741 aaacaattta acgatatgaa gaaaatgatg aaacaattca ctggtggcgg taaaggtaaa
    25801 aaaggtaaac gcaatcaaat gcaaaatatg ttaaaaggta tgaatttgcc gttttaatgt
    25861 ttaatataac acgctgattt ttgttgcaat gagatgtgca tcccgaaagt tatactaaag
    25921 aatataactt tatggggtgc atatttttag atacacaata ataattaaaa actgaaattg
    25981 ctgatttgct agaacatgag gttattgttg ctaatattaa taagttatat tgggggaacg
    26041 tgtgcggtag aagtggtacc attcatgtcc ttacgggata aattaactaa acacgtatgt
    26101 ggtgaaggct actatgaatt tttataacct attaacagtc taatttagta atcacatagt
    26161 tattattgta agaaaaaggt tcaataattt tctaggtaaa gaaaaaactc tttaca
//
LOCUS       DAHHYJ010000018        25915 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000018 DAHHYJ010000000
VERSION     DAHHYJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25915
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.018"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..232
                     /gene="mnhE2"
                     /locus_tag="NBK22_000591"
     CDS             <1..232
                     /gene="mnhE2"
                     /locus_tag="NBK22_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCD2413797.1"
                     /translation="KDMNPGLLSYETRLTSDWAITFLTILIIITPGSTVIRISQDSKK
                     FFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR"
     gene            229..531
                     /gene="mnhF2"
                     /locus_tag="NBK22_000592"
     CDS             229..531
                     /gene="mnhF2"
                     /locus_tag="NBK22_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCD2413798.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            506..943
                     /gene="mnhG2"
                     /locus_tag="NBK22_000593"
     CDS             506..943
                     /gene="mnhG2"
                     /locus_tag="NBK22_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCD2413799.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDAHTHASILLSSNEQNSTEALQLRAKKREEHRKKWYQND"
     gene            1283..3325
                     /locus_tag="NBK22_000594"
     CDS             1283..3325
                     /locus_tag="NBK22_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD2413800.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(4219..5148)
                     /locus_tag="NBK22_000595"
     CDS             complement(4219..5148)
                     /locus_tag="NBK22_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCD2413801.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIETVHGSMK"
     gene            complement(5145..5981)
                     /locus_tag="NBK22_000596"
     CDS             complement(5145..5981)
                     /locus_tag="NBK22_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD2413802.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(5975..6718)
                     /locus_tag="NBK22_000597"
     CDS             complement(5975..6718)
                     /locus_tag="NBK22_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD2413803.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            6840..7484
                     /locus_tag="NBK22_000598"
     CDS             6840..7484
                     /locus_tag="NBK22_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCD2413804.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(7564..8316)
                     /locus_tag="NBK22_000599"
     CDS             complement(7564..8316)
                     /locus_tag="NBK22_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCD2413805.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKPVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFIPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            8552..9316
                     /locus_tag="NBK22_000600"
     CDS             8552..9316
                     /locus_tag="NBK22_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCD2413806.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTHQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(9377..10171)
                     /gene="tagH"
                     /locus_tag="NBK22_000601"
     CDS             complement(9377..10171)
                     /gene="tagH"
                     /locus_tag="NBK22_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCD2413807.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            10518..11330
                     /gene="tagG"
                     /locus_tag="NBK22_000602"
     CDS             10518..11330
                     /gene="tagG"
                     /locus_tag="NBK22_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCD2413808.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            11429..12532
                     /locus_tag="NBK22_000603"
     CDS             11429..12532
                     /locus_tag="NBK22_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD2413809.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSDLSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKAMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKDKYGITNKLVLYVPT
                     YREDKADNRAIDKAYFEKCLPGYTLINKLHPSIEDSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLDIPTIFYVYDEGTYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            12529..13590
                     /locus_tag="NBK22_000604"
     CDS             12529..13590
                     /locus_tag="NBK22_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD2413810.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVINPTSIVLPGKL
                     DRLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLTEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDE
                     DFNRYIIEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKRF
                     LIQLKGRKFAHRTKSMLKR"
     gene            13653..14051
                     /gene="tagD"
                     /locus_tag="NBK22_000605"
     CDS             13653..14051
                     /gene="tagD"
                     /locus_tag="NBK22_000605"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCD2413811.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(14168..15463)
                     /gene="pbp4"
                     /locus_tag="NBK22_000606"
     CDS             complement(14168..15463)
                     /gene="pbp4"
                     /locus_tag="NBK22_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCD2413812.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDDTVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLTGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWAEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            15884..17611
                     /locus_tag="NBK22_000607"
     CDS             15884..17611
                     /locus_tag="NBK22_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2413813.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTSTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            17968..19197
                     /locus_tag="NBK22_000608"
     CDS             17968..19197
                     /locus_tag="NBK22_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD2413814.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            19452..19544
                     /locus_tag="NBK22_000609"
     CDS             19452..19544
                     /locus_tag="NBK22_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413815.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIANNEMKVN"
     gene            19722..20555
                     /locus_tag="NBK22_000610"
     CDS             19722..20555
                     /locus_tag="NBK22_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD2413816.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            20837..21634
                     /locus_tag="NBK22_000611"
     CDS             20837..21634
                     /locus_tag="NBK22_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2413817.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDGENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            21670..22674
                     /locus_tag="NBK22_000612"
     CDS             21670..22674
                     /locus_tag="NBK22_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD2413818.1"
                     /translation="MTNRENPTPLKFLSYIIGLSMILLITLFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            22671..23687
                     /locus_tag="NBK22_000613"
     CDS             22671..23687
                     /locus_tag="NBK22_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD2413819.1"
                     /translation="MISSNNKRRQLIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITTALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            23921..24889
                     /gene="dhaK"
                     /locus_tag="NBK22_000614"
     CDS             23921..24889
                     /gene="dhaK"
                     /locus_tag="NBK22_000614"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCD2413820.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKAKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVQTVIVRDDIAVTNEVQRRGVAGTVFVHKLAGYLA
                     EKGYSLTEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVGTLLDELYKEVTANDVILMVNGMGGTPLSELNIVTKYIQQNLAAR
                     TVNVAKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            24931..25515
                     /gene="dhaL"
                     /locus_tag="NBK22_000615"
     CDS             24931..25515
                     /gene="dhaL"
                     /locus_tag="NBK22_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCD2413821.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKDKLDDSSMQSLFKSTGTALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFITL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEELKNSETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            25508..25870
                     /locus_tag="NBK22_000616"
     CDS             25508..25870
                     /locus_tag="NBK22_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCD2413822.1"
                     /translation="MPKIILVSHSKEIASGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEVLTKLEDDALCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
BASE COUNT         8773 a         3721 c         4674 g         8747 t
ORIGIN      
        1 aaaagatatg aaccctggat tactttcata tgaaacaaga ctaacaagtg attgggcaat
       61 aacattttta acaattttaa ttattataac tccagggtct acagtaatac gaatttctca
      121 agactctaaa aagtttttta ttcatagtat cgacgtgtca gaaaaagaaa aagatagttt
      181 gttaagaagt attaagcatt atgaagactt aatattggag gtgtcgcgat gatacaaaca
      241 ataacacata ttatgattat tagttcactc attatttttg gaattgcatt aatcatctgt
      301 ttatttagat taattaaggg acctacaaca gcagatcgtg tcgttacatt tgatacaaca
      361 agtgctgtcg taatgtcaat tgtgggtgtg ttaagtgtac ttatgggcac cgtttctttc
      421 ttagattcaa tcatgctcat tgccattata tcttttgtaa gttctgtttc aatatcacgc
      481 tttattggtg gggggcatgt gtttaatgga aataacaaaa gaaatcttta gtcttattgc
      541 tgctgtgatg ttgttgttag gtagttttat tgctcttatt agtgcaatag gtatcgtgaa
      601 attccaagat gttttcttaa gaagtcacgc tgcgacaaaa agttcaactt tatccgtgtt
      661 attaacttta atcggtgtat taatttattt tattgtgaat acaggatttt tcagtgtgcg
      721 tttattactg tcacttgttt ttattaattt aacttcacca gtcggcatgc acttagtcgc
      781 tcgcgctgct tatcgcaacg gcgcttatat gtatcgaaaa aatgatgctc acacacatgc
      841 atcaatatta ttaagttcaa atgaacaaaa ctctacagaa gcattacaat tacgtgctaa
      901 aaaacgagaa gagcatcgta agaaatggta tcaaaacgat tgaagtgttg gctaaattgt
      961 taagcataac aatgcttttg aaaatctgtt ttcaaaatta atcattaaaa gagtactttg
     1021 aaattggtta gtcgatttta aggtgctcat tttttgtatt ataagttaat gttgttatgt
     1081 gaaagcaagt tgtttattta tacaaacaat tagtgataaa ataaaattaa ataattgcaa
     1141 atttaagtac tgcaatcgta gacatatatc tatattaaag tgcttttgtg gtcgctaact
     1201 caatttatcc tctaaagtat aattacaacg agatgtgata cttaattttc aattaaacaa
     1261 tactttttag agaggtgaaa gtttggaaat atttgaaaca attcttatat ttatagctgt
     1321 tgtgatacta agttcgtttg tccatacttt catacctaaa gtacccctag catttataca
     1381 aattttcttg ggcatgttac tatttattac cccaatccct gttcaattta attttgattc
     1441 tgaattgttt atggtaacaa tgattgcgcc tttgttattt gtagaaggtg ttaatgtttc
     1501 tagagtccat ttaaggaaat atattaagcc agtgatgatg atggcattag gattagtcat
     1561 tactactgtg ataggtgtag gtttatttat tcattggatt tggccagatt tacctattgg
     1621 agcagcattt gcaattgctg ccattctttg tcctactgat gcagtagcag tgcaagcaat
     1681 cactaaagga aaggtcttgc caaaaggagc aatgacaatt cttgaaggtg agtcattatt
     1741 gaatgatgct gctggtatta tttcatttaa aatagctgtt ggagtattag ttacaggtgc
     1801 tttttcactt gttgatgctg ttcagttgtt tttaattgca tcaattggtg gcgcagtggt
     1861 tggtttactt ataggtatgg cattagtaag gttccgacta acattgatgc gtcgaggata
     1921 tgaaaacatt aatatgttta caattattca attgttaaca ccatttgtta cgtatttaat
     1981 tgctgaattg tttcacgcat caggaatcat tgcagcagta gttgcaggac ttgtacatgg
     2041 tttcgaacgt gacagaatta tgcaagtacg tacacaactg caaatgagtt acaatcatac
     2101 atggaatata ctaggttatg ttttaaatgg ctttgttttt tcaatattag gatttttagt
     2161 acctgaagtt attattaaaa ttatcaaaac agaaccgcac aatttaatct ttttaatagg
     2221 catcactatt gttgttgctt tagctgtcta tctatttaga tttgtttggg tttatgtctt
     2281 atatccttat ttttatttag ccatcagtcc attccaaaaa atgatgacta aaaatgatga
     2341 tgataatcca acgactgaga aaccaccaaa gcgaagttta tacgctttaa ttatgacgtt
     2401 atgtggtgtg catggaacaa tttctttagc aatcgcatta acgttaccgt attttttagc
     2461 agggcatcat gcttttacgt atagaaacga cttattattt attgcatctg gtatggttat
     2521 tattagtttg gtagttgcgc aagtattatt gccattatta acgaaacctg cacctaaaac
     2581 agtaattggc aatatgtcgt ttaaagttgc tagaatttat atattagaac aagttattga
     2641 ttatctaaat caaaaatcta ctttcgaaac aagttttaaa tatggtaacg tgattaaaga
     2701 atatcatgat aaattagcat ttttaaaaac tgtagagaaa gatgatgaaa actctaaaga
     2761 attagaacgt ctacaaaaaa ttgcttttaa tgtagaaaca aaaacattag agtctttagt
     2821 agatgaagga caaataacga atagtgtact tgaaaactat atgcgttatg ctgaaagaac
     2881 acaggtatat agacaagcat cattaataag aagaatgatt gtattattac gaggtgcttt
     2941 attaaaacga agagtacaaa cgagagtgaa ctccgcatct tcacttagtg ttacggataa
     3001 cttaatggaa ttaaataaaa ttaataaatt agtccattat aatgtggtta gtcgtttgtc
     3061 taaggaaaca acaaaagata atacacttga agttggaatg gtttgtgacg gttatttaat
     3121 gcgaattgaa aacttaacac catcaaattt cttcaactca gcaagtgaag atacgattac
     3181 taaaattaaa ttaaatgcat tgagagaaca acgtcgcatt ttacgtgaat tgatagatac
     3241 agacgaagta tctgaaggta ctgctttgaa attaagagag gcaatcaatt acgatgaaat
     3301 ggttattgta gatagtatga cgtagttcct aattatgcta aaagggattg atgaaaaact
     3361 gaagggcttt tcatcaatcc cttttatttt atgggaattg aatagatagt tttaaactat
     3421 acgaattatt aatatttgag atttaattga aataagtttt aaaaattgga ggagatagat
     3481 taagcgaagt catttaaagg tgaagttaag tgtattcaca aaaaatagcc acactcatat
     3541 gacatcggat gagtgtggct taaggatcta tggggggagg aaaccataga tgtttacttt
     3601 gataggccag attaaatatc aaagtatgcg attatttata gcttgatgca aaagtggtat
     3661 gcctattaaa agttactgca catagctttt aatattccgt tcaaaggaaa ggggcataca
     3721 attgaacaat ctgtaatagt acttttaacc agctatgcta aaagtctagt agggagaaca
     3781 gttgtccaat cacataagaa cctctaactt cgttagtacg attaagaaaa gctttttagt
     3841 tagtatgtaa tacaatttat tgacgcgcgt gaatctcttt tataagagtg tgtagggaat
     3901 ggcgttgtat aaattgtatt agaagaactt ctaacgcatc tctgtggtta aaagagatga
     3961 agggaacgac agtttaatta aaactgcata agaacttcta gcttttctct ttcgttcaaa
     4021 gagaagttct aataccacca tatcgtgcga tcgggaatgg tatatatatt aataggaggg
     4081 taatatatat ttaacgcacg atatgggact attagccttc gactttgtta tgttgatgtg
     4141 tggcctaaaa tattggagat accaatattt taggttgcat caacatcaat tcatcttact
     4201 tcattaaaac acagcgtgtt atttcatgct tccgtgtaca gtttcaatat ttgatttcat
     4261 cattttgtag taagagtcac ctttagtgcc ttctttaccg attgaatctg tgtacacttc
     4321 accaaagata tctttcttcg tttcttcaga taaactttcc attgctttct tatcaacact
     4381 tgtttctact aataagtgtt ttaatttgtg ctttttaaca aactcaatag cttgtctcat
     4441 ttgttcaggt gtaccttgtt tttcagtgtt aatttcccaa atataacctg gtgtaatacc
     4501 gtattgtttt gagaagtact tgaaggcacc ttcacttgta atcatggcac gttgttcttt
     4561 tggaatgtca ttaaatttgt ctttactgtc attatttaat ttttccaatt gagcaatgta
     4621 tttgttacct tgcttttcat aatctgcttt atgttttttg tcgttatcga taaatgtttg
     4681 ttgaattgtt tttacgtatt taataccgtt atctaaactt aaccatgcgt gtggatcttg
     4741 tttatctttg ttgccttctt caccgtttaa atagataggt ttaacatctt ttgatactgc
     4801 gataactttt ttatctttta atgatttacc agcctgttct aaggcttttt caaaccaacc
     4861 gttaccagtc tctaaattta atccgttgta taaaataacg tcagcgtcag ttaacttttt
     4921 aatatcttta ggtttaactt catattcatg aggatcttga ccaacaggta caatactatg
     4981 aatatcgacg ttgtctccac caacattttt agccatatca tataaaattg aattcgtcgt
     5041 tactactttt aatttgccgt ttgacttatc actgctttgt ttaccaccag taccacatgc
     5101 agcaactaga agtaataagg ctaataataa aggtactaat tttttcatgt taaacttcct
     5161 cgtttctttc tattcgtaaa ttttgtgaaa aataatgtga tgatataaat tacaaacgta
     5221 caaagtacga ttgtcgcacc actaggaatg ttgtaaatat agctgtaata aagtccgaca
     5281 attgaactta tgacacttat taaacttgct ataatcatca ttgagtatag ttttttacta
     5341 attaaaaatg ctgtagatgc aggtgtaatt aataatgcaa ctacaagaat aatacctacc
     5401 gtttgaatac ttgctactgt tactaatgag agtaacaaca tcacaaagta atgtaataac
     5461 gtcgtattta gaccactcat tctactaaac gttggatcga atgtagaaat cattaatgga
     5521 cgatagaaaa taatgattag aataaggacg attgaaccaa tcacaatagt tgttaaaaat
     5581 gcactatttg tgattgccag taaattacca aacagaatat ggtacaaatc tgtcgtagtg
     5641 tttattaagc taataataat aatccccgaa gctaagaaag cggtaaaact aattccaata
     5701 gcggcgtcag gtttcgtttt actactagat gtgatataac cgataaaaat acttgcgatc
     5761 ataccagtta taagtgcgcc tacaaacatt ggaataccaa ataagaatga tagggcaaca
     5821 ccaggtaata ctgcgtgact cattgcatct cccattaatg aaagaccacg taatacaatt
     5881 aaactaccaa ctgtaccaca aactatccct acaataattg aagttatcaa tgctcgattc
     5941 aagaattgat atgtaaataa atgttcgaca aactctaaca tgttatattg ctcctttgac
     6001 tagggtcact acagtcagtg ctactcataa atgtttcgtt taagcgagtg acactcatag
     6061 cctcttcact atcaccaaag tatcgtaatg tttgatttaa tagaataatg cgatcaaagt
     6121 attgctttgc ttttgataga tcatggtgga tgataagaat aagttttcct tgttgtttta
     6181 agttctcgat ttttgtcatg attaattttt cgctactaaa atcaattccg acaaacggct
     6241 catctagaaa ataaacttca ctttcggaca tcaatgctct tgctactagc acacgttgta
     6301 attgtccacc acttaattct gaaatttgtc gatgacgtaa agattctaat tctaaatcgc
     6361 ttaataactg tttgagttta tcccttgctg atttattagg tcgtctaaac catccaattt
     6421 ctttgtagca acctgataaa atcacttgtt ccacacttat aggaaaatct aaatcaatat
     6481 gtgctttttg tggaatatat gtaatatgtt gcagttgttg ttgtataggt ttgttatata
     6541 acaatttagt accggtagca ttaaattcac caattaaaga cttgataagg gaagatttac
     6601 cagcaccatt cgggcccatg ataccaatta tttcgccgcg tactggtatc gataaggaaa
     6661 tgtttttaag tacatgctta ttacctaaaa acagatttaa atcttttgtt tctaacaaac
     6721 gtttatacct cctaattaaa agtttaggct aacctaatta attgtataat aaactgagaa
     6781 tatttatcat gtcaagtaaa ttcgtgatat aatatagaca atgtatgtga ggtgaaagta
     6841 tgttaactga agaaaaagag gactatttaa aggcaatcct tacgaataat ggcgataaaa
     6901 actttgtgac aaataaaatc ttatctcaat ttttaaatat taagcctcca tctgtaagtg
     6961 aaatggtagg acgtcttgaa aaagcaggct atgttgaaac aaaaccatac aaaggtgtta
     7021 gattaacaga ggatggttta acgcatacgc ttgatatcat taagagacat cgactattag
     7081 aattattttt aatagaaata ttgaaataca attgggaaga agtacatcaa gaagcagaaa
     7141 ttttagaaca tcgaatttca gatttatttg ttgaaaggct ggatagcctg ttaaatttcc
     7201 cagaaacttg cccgcacggc ggtgtgattc ctagaaataa tgaatataaa gagaaatata
     7261 taacaacgat tttgaattat gaacctggtg atatcgttac aatcaaacgt gtgagagata
     7321 agaccgattt gctaatatat ttgtctagta aagatatttc tattggtaat gaagtggaaa
     7381 ttgtatcgaa agatgaaatg aataaagtaa ttatcattaa acgtaatgat aatgtaatta
     7441 ttgtcagtta cgaaaatgca atgaacatgt ttgctgaaaa ataaaataaa gaagccataa
     7501 agatatccat gattgaactg ataaagacat atggataatt gctttaggct tcttttttat
     7561 tagttaattt atcaagtgag tatatttgag taaaatattc actgcataaa gattgaagat
     7621 aatccatatt gtactataaa tgaagatagg tacatgactg agttctttaa gtgcactacc
     7681 atcccactgt ggactcggac gctggaaagt caatttagca atcgtccaac tagattgtag
     7741 aacttcgcct aataatacac ctaaaatata ttgataactc attgtgacaa gtagttgaat
     7801 ttctactata ttttcatctt ttaatataaa atacaacatg atagaaatta aagttataac
     7861 aacaatgggt gagccttttc tagatgttaa aattaaaaaa taaataaata tcaataaata
     7921 ggtaaatata aagaaactag gtatctgata atggctcgac gctaaaccta tcaataacat
     7981 aataggtggt ataaaataac caccaatcgt tgtaagccat tgacctgcta gatgtctaga
     8041 ttgtgtaatt gcgaatcctt gttgtaatgt ctgttgtcgc tctcgtggac ttgttacaat
     8101 gactaaatct tttgcacggc caccagcgag tttattaaac agtacatgac caaattcatg
     8161 tgttaaaaca gggatatagt ttaaaatgac atctaaatag ttcaaaacag gcttatgtct
     8221 atattgatga atagcaatat aacaagctgc aacaataacg ataatgtata tattaagttg
     8281 aattgtcgta ttaaaaaagt ttgataaata attcattgtt aacctcatat aagatattaa
     8341 tttaaagttt gcttagcact tattataaat gatattggca tcaatagcgt tagactttag
     8401 acttacctta gttaaactaa ttttaatttt tgaaaaggtg aatatgtgtt aaaataaagc
     8461 aaaatcattt cgatataaat aggatgaata taaatactgt taatattgat tacactaaca
     8521 taataatgaa ataagatagg agattcctgt tatgactgtt gaagaaagat ccaatacagc
     8581 caaagttgac attttagggg tcgattttga taatacaaca atgttgcaaa tggttgaaaa
     8641 tattaaaacc ttttttgcaa atcaatcaac gaataatctt tttatagtaa cagccaaccc
     8701 tgaaatagtg aattacgcga cgacacatca agcgtattta gagttaataa atcaagcgag
     8761 ctatattgtt gctgatggga caggagtagt caaagcttcg catcgtttaa agcaacctct
     8821 agcgcatcgt atacctggta ttgagttgat ggatgaatgt ttgaaaattg ctcatgtaaa
     8881 tcatcaaaaa gtatttttgc taggggcaac taatgaagtt gtagaagcgg cacaatatgc
     8941 attgcaacaa agatatccaa acatatcgtt tgcacatcat cacggttata ttgatttaga
     9001 agatgagaca gtagtaaaac gaattaaact gtttaaacct gattacatat ttgtaggtat
     9061 gggattccct aaacaagaag aatggattat gacacatgaa aaccaatttg aatctacagt
     9121 gatgatgggc gtaggtggtt ctcttgaagt atttgctggg gctaaaaaga gagcgcctta
     9181 tatctttaga aaattaaaca ttgaatggat atatagagca ttaatagatt ggaaacgtat
     9241 tggtagatta aagagtattc ctatatttat gtataaaata gccaaagcca aaagaaaaat
     9301 aaaaaaggcg aaataatcat gatgacaaaa ataaaaccga ggaaatcctt gaatggagat
     9361 tctcggtttt ttcggtttat ttaataacga agcgggactc atcgagtttg tttctaaatt
     9421 ctttttgttc ggctttggat ttctttttaa aatcgttaag gaaagcttca tatttaggta
     9481 atacatcatc aagttcaccg taatctttta actttccgcc ttcaatccaa gcaatcttag
     9541 tacaaaattg tctcacttgt cctaagttat gactaacgaa aaagatggtt ttgttttgct
     9601 ctttaaactc gtaaatttta tctaaacatt tttgtgcaaa agtttggtca cctacagata
     9661 aagcttcgtc aatgactaag atatctggat taactgtgat attaattgaa aaaccaagtt
     9721 ttgcacgcat accacttgaa tactttttaa ctggttgata aataaactca ccaagttcac
     9781 taaattcaat aatcttaggt gtcatcgctt taatttcttt tcgcttaaag cccatacata
     9841 acattttaaa ttcgatattt tcaatccctg taagttgtcc actcaagcca gcactaattg
     9901 cgataacgct gacttcacca ttacgatcca ctttgccaac agtaggcgac aaagaaccgc
     9961 caatgatatt gctcaacgtt gatttgccgg aaccattgat gccaacaagc cctatgacgt
    10021 cgccttcata tgcttttaaa ctaatgtcat ctaaagcgaa aaatgttttg tttttatgtt
    10081 tgggaatgag cgcatctttc atacgttctt tatttgtacg ataaatacga tattcttttg
    10141 ttacattttt aatgtttacc gaaacgttca tttgtagacc ttccttattc acatttatct
    10201 agattataat atactactca acagttgtta aattttaaaa cctgttgtaa agtgtataga
    10261 agattttgtt attatcagag tgggtgtttt gacacaaaat gttaatcatc aatgataaca
    10321 atgatattta aaaactaaac ttatttcaac ttacatgatt gtatactata atgtatttgt
    10381 aataaactaa tattttaaag aactagacaa taattttgat agcatccatg tatagtgata
    10441 gtatttacaa caattattat aatactattt agttaagtag agaaatagtt aaacatttga
    10501 aagtgtggtt taatggaatg tcagcaatag gaacagtttt taaagaacat gtaaagaact
    10561 tttatttaat tcaaagactg gctcagtttc aagttaaaat tatcaatcat agtaactatt
    10621 taggtgtggc ttgggaatta attaaccctg ttatgcaaat tatggtttac tggatggttt
    10681 ttggattagg aataagaagt aatgcaccaa ttcatggtgt accttttgtt tattggttat
    10741 tggttggtat cagtatgtgg ttcttcatca accaaggtat tttagaaggt actaaagcaa
    10801 ttacacaaaa gtttaatcaa gtatcgaaaa tgaacttccc gttatcgata ataccgacat
    10861 atattgtgac aagtagattt tatggacatt taggcttact tttacttgtg ataattgcat
    10921 gtatgtttac tggtatttat ccatcaatac atatcattca attattgata tatgtaccgt
    10981 tttgtttttt cttaactgcc tcggtgacgt tattaacatc aacactcggt gtgttagtta
    11041 gagatacaca aatgttaatg caagcaatat taagaatatt attttacttt tcaccaattt
    11101 tgtggctacc aaagaaccat ggtatcagtg gtttaattca tgaaatgatg aaatataatc
    11161 cagtttactt tattgctgaa tcataccgtg cagcaatttt atatcacgaa tggtatttca
    11221 tggatcattg gaaattaatg ttatacaatt tcggtattgt tgccattttc tttgcaattg
    11281 gtgcgtacct acacatgaaa tatagagatc aatttgcaga cttcttgtaa tatatttata
    11341 tgacgaaacc ccgctaacca ttaataaatg gaagtggggt tcatttttgt ttataattta
    11401 agtaaataac atattaagtt ggtgtattat gaacgtttta ataaagaaat tttatcattt
    11461 ggtagttcga atactttcta aaatgattac gcctcaagtg attgataaac cgcatatcgt
    11521 atttatgatg acttttccag aagatattaa acctatcatc aaagcattaa ataattcgtc
    11581 gtatcagaaa actgttttaa caacaccaaa acaagcgcct tatttatctg atcttagcga
    11641 cgatgttgat gtgatagaaa tgactaatcg aacattggta aaacaaatta aggctttgaa
    11701 aagcgcgcag atgattatta tcgataatta ttacctatta ctaggtggat ataataagac
    11761 ttctaatcaa cacattgttc aaacgtggca tgcaagtggt gcattaaaaa actttggctt
    11821 aacagatcat caagtcgatg tgtctgacaa ggcaatggtt cagcagtacc gtaaagttta
    11881 tcaagcgacg gatttttact tagtgggttg tgaacaaatg tcacaatgtt ttaaacagtc
    11941 tttaggtgca acagaagagc aaatgctgta ttttgggctt ccgagaatta ataaatatta
    12001 cacagctgat agagcaacgg ttaaggcaga gttaaaggat aaatatggaa ttacaaataa
    12061 gttggtatta tatgtaccaa catatagaga agataaagca gataataggg ctattgataa
    12121 agcttatttt gaaaaatgtt taccaggata tacactgatt aataaattac atccatcaat
    12181 tgaagattca gacattgatg acgtatcttc aatcgacacg tctacattaa tgctaatgtc
    12241 agatataatt attagcgact atagttcgct gccaatagaa gctagcttgt tagatattcc
    12301 aactatattt tatgtgtatg atgaaggaac atatgataaa gtgagaggcc tgaatcaatt
    12361 ttacaaagca ataccggata gctacaaagt gtatactgaa gaagatttaa taatgacgat
    12421 acaagaaaaa gaacatctat taagtccgtt atttaaagat tggcataagt ataatactga
    12481 taaaagttta catcagctca cagaatatat agataagatg gtgacaaaat gaggcttacg
    12541 ataatcatac ctacatgtaa taatgaggca acaattcgac aattgttaat atctattgag
    12601 agtaaagaac actatagaat cctttgtatt gatggtggtt ctactgatca aacaattcct
    12661 atgattgaac ggttacaaag agaactcaag catatttcat taatacaatt acaaaatgct
    12721 tcgatagcta cgtgtattaa taaaggtttg atggatatca aaatgacaga tccacatgat
    12781 agtgacgcat ttatggtcat aaatccaaca tcaatcgtat tgccaggtaa attagatagg
    12841 ttaactgcag ctttcaaaaa taatgataat attgatatgg taatagggca gcgagcttac
    12901 aattaccatg gtgaatggaa attgaaaagt gctgatgagt ttattaaaga taatcgaatc
    12961 gttacattaa cggaacaacc agatttgtta tcaatgatgt cttttgacgg aaagttattc
    13021 agtgctaaat ttgctgaatt acagtgtgac gaaactttag ctaacacata caatcacgca
    13081 atacttgtca aggcgatgca aaaagctacg gatatacatt tagtttcaca gatgattgtc
    13141 ggagataacg atatagatac acatgctaca agtaacgatg aagattttaa tagatatatc
    13201 atagaaatta tgaaaataag acaacgagtc atggaaatgt tactattacc tgaacaaagg
    13261 ctattatata gtgatatggt tgatcgtatt ttattcaata attcattaaa atattatatg
    13321 aatgaacacc cagcagtaac gcacacgaca attcaactcg taaaagacta tattatgtct
    13381 atgcagcatt ctgattatgt atcgcaaaac atgtttgaca ttataaatac agttgaattt
    13441 attggtgaga attgggatag agaaatatac gaattgtggc gacaaacatt aattcaagtg
    13501 ggcattaata ggccgactta taaaagattc ttgatacaac ttaaagggag aaagtttgca
    13561 catcgaacaa aatcaatgtt aaaacgataa cgtgtacatt gatgaccata aactgcaatc
    13621 ctatgatgtg acaatatgag gaggataact taatgaaacg tgtaataaca tatggcacat
    13681 atgacttact tcactatggt catatcgaat tgcttcgtcg tgcaagagag atgggcgatt
    13741 atttaatagt agcattatca acagatgaat ttaatcaaat taaacataaa aaatcttatt
    13801 atgattatga acaacgaaaa atgatgcttg aatcaatacg ctatgtcgat ttagtcattc
    13861 cagaaaaggg ctggggacaa aaagaagacg atgtcgaaaa atttgatgta gatgtttttg
    13921 ttatgggaca tgactgggaa ggtgaattcg acttcttaaa ggataaatgt gaagtcattt
    13981 atttaaaacg tacagaaggc atttcgacga ctaaaatcaa acaagaatta tatggtaaag
    14041 atgctaaata aattatatag aactatcgat actaaacgat aaattaactt aggttattat
    14101 aaaataaata taaaacggac aagtttcgca gctttataat gtgcaacttg tccgttttta
    14161 gtatgtttta ttttcttttt ctaaataaac gattgattat catatgaaca attagtgcta
    14221 atccagcgac aaggcatgca ccaccaatga tagtgaataa tggatgttct gcccacatac
    14281 ttttagcaac agtatttgcc ttttgaataa ttggctgatg aacttctaca gttggaggtc
    14341 cataatcttt attaataaat tctcttggat agtccgcgtg tactttacca tcttcgacta
    14401 caagtttata atctttttta ctaaaatcac ttggtaaaac atcgtaaaga tcgttttcaa
    14461 cataatattt cttaccattt atcctttgct cacctttaga caatattttt acatatttat
    14521 actgatcaaa tgagcgttcc attaatgcat tccccatcat attacgttgc ttctcgccac
    14581 caaggttttt atagtctccc gcacccatga taacttgatt aattctaaat ttacctcgtt
    14641 tggtagtaat cgtatggttg taatttgctg tatcacttga tccagttttt aaaccatctg
    14701 taccagtcaa actcattttt gcaccttcca atgaaaagtt gaatgtgtaa tacgtaactg
    14761 catgcgttgt tggtgctaac tgctttgtaa agtctaatat tttaggtgtc tctttaatca
    14821 cgtgtaaatc taaaatggcg tagtctctag cagtcgttac agtacgttct tggtctttat
    14881 actttgttgg tgcaaatgta cgtaatcttg aattttcagc acccgttgga ttgacgaaat
    14941 gtgtattttt cattccgata gctttagctt tgttattcat taaatcaacg aaatcgctgg
    15001 tgttttttga aaccttctta gctaaaatta atgccgcggc attactagaa ttagatactg
    15061 taatttgtaa taggtctgcg attgtccata cttgtccagg atatagtttc gtattactca
    15121 actcaggtag tgtagacata atatattctt tgttcgtcat tgtgacagtg tcatcaagtg
    15181 aaagctgccc cttatttaca gcttccaatg ttaagtacat tgtcattaat ttagtcatag
    15241 acgctggatt ccacttagta tcgatattgt attgatacag taattgtcca gtttgactta
    15301 cattaacagc actcgtcggt tcgtatgcag ctgacaaacc tgcataacca tattgatttg
    15361 ctgcttgtac aggggttacg tcactgttag cagcttgtgc atatggtgtc ataatactta
    15421 atgttaaaca taaaatgatg ataatagata ttaaattttt cataaagcgt taatcttccc
    15481 ttttccaatt cttaaatatt ccctaaaagc aatggttatt cctacttacg gaaatcattg
    15541 ctaattcact tcaccttaat taaattgttg aaaataaagt tttctgcagt taatttgaaa
    15601 aataatgcaa atatattacg tgtgtagcta aaggtgttat aatgtttgta cgaagagcaa
    15661 acttactcaa aagcgattaa ttttcatgtt ttaatataaa gactttgaga agttattaca
    15721 aaaaatgcaa tagaaatatt ctatcatata aatgttatga gcggtatttt ggggcaacac
    15781 tttatttgat ttttaaagtt ttgttgggag aaagtatatg atagaaatgc atgtatctat
    15841 ctaaatgaat taactataaa tttcaaacag aagaggtaaa actatgaaac gagaaaatcc
    15901 attgtttttc ttatttaaaa aactatcatg gccagtgggt cttatcgttg cagctatcac
    15961 tatttcatca ctagggagct taagtggact attagtgcca ctgtttactg gtcgaattgt
    16021 agataaattt tccgtgagcc atatcaattg gaatctaatc gcattatttg gtggtatctt
    16081 tgtcatcaat gctttattaa gcggattagg tttatattta ttaagtaaaa ttggtgaaaa
    16141 aattatttat gcgatacgct cagttttatg ggagcatatc atacaattaa aaatgccatt
    16201 ctttgacaaa aatgaaagtg gtcaattaat gagtcgatta actgacgata cgaaagtgat
    16261 aaatgaattt atttcacaaa agctacctaa cttattacca tcaatcgtta cattagttgg
    16321 gtcactaatc atgttattta ttttagattg gaaaatgaca ttattaacat ttataacgat
    16381 accgatattc gttttaatta tgattcctct aggtcgtatt atgcaaaaga tatcgacaag
    16441 tacacaatct gaaattgcaa acttcagtgg tttgttaggg cgtgtcctaa ctgaaatgcg
    16501 tcttgttaaa atatcaaata cagagcgtct tgaattagat aatgcacata aaaatttgaa
    16561 tgaaatatat aaattaggtt taaaacaggc taaaattgcg gcagttgtac aaccaatttc
    16621 aggtatagtt atgttgctaa caattgcaat tattttaggt tttggtgcat tagaaattgc
    16681 gactggtgca atcactgcag gtacattaat tgcaatgata ttttatgtta ttcagttatc
    16741 tatgccttta atcaatcttt caacgttagt tacagattat aaaaaggcag tcggtgcaag
    16801 tagtagaata tacgaaatca tgcaagaacc tattgaaccg acagaagctc ttgaagattc
    16861 tgaaaatgta ttaattgatg acggtgtatt gtcatttgaa catgtagact ttaaatatga
    16921 tgtgaagaaa atattagatg atgtgtcgtt ccaaatccca caaggtcaag tgagtgcttt
    16981 tgtaggtcct tctggatctg gtaaaagtac gatatttaac ctgatagaac gtatgtatga
    17041 aattgagtca ggtgatatta aatatggcct tgaaagtgtc tatgatatcc cgttatctaa
    17101 gtggcgacgc aaaattggat atgttatgca atcaaattcg atgatgagtg gtacaattag
    17161 agacaatatt ttatacggaa ttaatcgtca tgtttcagat gaagaactta ttaattatgc
    17221 taaattagcg aactgtcatg attttatcat gcaatttgat gaaggatatg acacgcttgt
    17281 aggtgaacga ggattgaaac tgtctggcgg acaacgtcaa cgtattgata ttgctagaag
    17341 ttttgttaaa aatcctgata ttttgttact tgatgaagca acagctaatc tcgatagtga
    17401 aagtgaattg aaaattcaag aagctttaga aacattgatg gaaggtagaa caacgattgt
    17461 cattgcgcat cgtttgtcta cgattaaaaa agccggtcaa attatattct tagacaaagg
    17521 acaggtaaca ggtaaaggta cgcattcaga actgatggca tcacatgcga agtataaaaa
    17581 ctttgtagtg tctcaaaaat taacagatta attttatata taagtaagct tggagcaaat
    17641 acacatatac catcgaggaa attaaattgt ggcacattga tggatataga tgttaataaa
    17701 ttgcttcaag cttttgtcta tttttaatca tttgagaagt tacgacataa taattcttaa
    17761 attaatgaaa tcgatatttt aagaaaaaaa tgctcatggt ataatacaag ttataagcaa
    17821 acatacatat attaaatact gtagccacga gtcataattc ttcatatttt acatagcaat
    17881 ttaactgatt ttagagtcca cggtacagaa gtttgatatt tcaatgtttc taaattttta
    17941 aaaaattaaa tcataggtgg gtgccaaatg tttttattaa tcaacattat tggtctaatt
    18001 gtatttcttg gtattgcggt attattttca agagatcgca aaaatatcca atggcaatca
    18061 attgggatct tagttgtttt aaacctgttt ttagcatggt tctttattta ttttgattgg
    18121 ggtcaaaaag cagtaagagg agcagccaat ggtatcgctt gggtagttca gtcagcgcat
    18181 gctggtacag gttttgcatt tgcaagtttg acaaatgtta aaatgatgga tatggctgtt
    18241 gcagccttat tcccaatatt attaatagtg ccattatttg atatcttaat gtactttaat
    18301 attttaccga aaattattgg aggtattggt tggttactag ctaaagtaac aagacaacct
    18361 aaattcgagt cattctttgg gatagaaatg atgttcttag gaaatactga agcattagcc
    18421 gtatcaagtg agcaactaaa acgtatgaat gaaatgcgtg tattaacaat cgcaatgatg
    18481 tcaatgagct ctgtatccgg agctattgta ggtgcgtatg tacaaatggt accaggagaa
    18541 ctggtactaa cggcaattcc actaaatatc gttaacgcga ttattgtgtc atgcttgttg
    18601 aatccagtaa gtgttgaaga gaaagaagat attatttaca gtcttaaaaa caatgaagtt
    18661 gaacgtcaac cattcttctc attccttgga gattctgtat tagcagcagg taaattagta
    18721 ttaatcatca tcgcatttgt tattagtttt gtagcgttag ctgatctatt tgatcgtttt
    18781 atcaatttga ttacaggatt gatagcagga tggataggca ttaaaggtag tttcggttta
    18841 aaccaaattt taggtgtgtt tatgtatcca tttgcgctat tactcggttt accttatgat
    18901 gaagcgtggt tggtagcaca acaaatggct aagaaaattg ttacaaatga atttgttgtt
    18961 atgggtgaaa tttctaaaga tattgcatct tatacaccac accatcgtgc ggttattaca
    19021 acattcttaa tttcatttgc aaacttctca acgattggta tgattatcgg tacattgaaa
    19081 ggcattgttg ataaaaagac atcagacttt gtatctaaat atgtacctat gatgctatta
    19141 tcaggtatcc tagtttcatt attaacagca gctttcgttg gtttatttgc atggtaatat
    19201 gtcgaagagt gactatgata atacatttta actaataaat atgtccaggc atgtcgtcta
    19261 ttgatatagg tgagatgctt ggactttttt attattgata taaaggtatt taaatatttt
    19321 taaagttacc gaaattgaag cattataaaa accaagtgca catggtaata cacttggctt
    19381 ttatgggaaa tgaatattat tgtacatatg acagtaagga ctaggtacag tcatagtact
    19441 tcgagcaaaa tttgttttgt tattataaac aacacaaagg agataacttc tctattgaag
    19501 aagttaaaaa cattatagca aacaatgaaa tgaaagtaaa ttaaaaattc agaatatttt
    19561 taattattat attgtgagtg atatttatta gggaaagtta ttcttcatat aaactagtta
    19621 aatagtaaat ctttgttaga actctttcca tagagttgtg acagtatttt tgttgtgcta
    19681 ctatttttat agtctaataa atatataaag gggatggttt cgtgaataaa acggttaaag
    19741 atttaatact agttgtctta ggttcattta tctttgctgc aggtgtaaat gcatttatta
    19801 tttctggtaa cttaggtgaa ggcggggtta caggtttagc aattatttta tattatgcgt
    19861 ttcatatttc accagccatc actaatttct tggtcaacgc agtattgatt gccataggtt
    19921 ataaattttt gagtaagaga agtatgtact taactattct tgtaacaatt cttatttcaa
    19981 tatttttgag tttaacagaa tcatggcaag tagaaactgg aaacagcatt gtgaatgcca
    20041 tttttggtgg tgtaagcgtt ggactaggaa tcggagtaat tatccttgca ggcggtacaa
    20101 cagcaggtac aacaattttg gcgagaattg caacgaaata cctcgatgta agcacgccat
    20161 atgctttgct tttcttcgat atgatcgttg ttgcaatttc acttacagtt attccacttg
    20221 ataaagtatt agtaacagta atatcacttt atataggaac aaaagtgatg gaatatgtca
    20281 tagaaggttt aaacactaaa aaagctatga cgattatttc aactaatccc gacaaacttg
    20341 ccaaagcaat agacgagcaa attggaagag gtttaactat tttaaacgga catggctatt
    20401 atacgcgtga agaaaaagat gtcttatacg ttgttatttc taaaacacaa gtttcaaaag
    20461 caaagcgatt aattaaacaa atcgataaag atgcattcct cgtaattcat gatgtaagag
    20521 atgtctatgg taatggcttt cttgcagatg aataaataaa tggtatgagc acacatactt
    20581 aaatagaagt ccacggacaa gtttttgaac tatgaagact tatctgtggg cgttttttat
    20641 tttataaaag taatatacaa gacatgacaa atcgagctat ccaatttaaa aagtaatgtt
    20701 agtcaataag attgaaaaat gttataatga tgttcatgat aatcattatc aattggggtg
    20761 cctttgaaaa ttgataattt aaaaatagaa attatttttt ataaacagaa agaattttat
    20821 tgaaagtagg gaaattatga atcgtttgca tggacaacaa gttaaaattg gttacgggga
    20881 taacacgatt ataaataaat tagatgttga aataccagat ggcaaagtga cgtcaatcat
    20941 tggtcctaac ggctgcggga aatctacttt gctaaaggca ttgtcacgtt tattggcagt
    21001 taaagaaggc gaagtatttt tagatggtga aaatattcat acacaatcta cgaaagagat
    21061 tgcaaaaaaa atagccattt tacctcaatc acctgaagta gcagatggct taactgttgg
    21121 ggaattagtt tcatatggtc gttttccaca tcaaaaagga tttggtagat taactgctga
    21181 ggataagaaa gaaattgatt gggcaatgga agttacagga actgatacat tccgacaccg
    21241 ttcaatcaat gatttaagtg gtggtcaaag acaacgtgtt tggattgcaa tggcattagc
    21301 acaaagaact gatattatct ttttagacga accaacaaca tatttagata tctgtcatca
    21361 attagaaata ctagaattag ttcagaagct aaatcaggaa caaggttgta caattgtcat
    21421 ggttcttcat gatatcaacc aagcgattcg tttctcagat catcttattg cgatgaaaga
    21481 aggggatatc atcgctacag gttcaacaga agacgtatta acacaggaaa tattagaaaa
    21541 agtttttaat attgatgttg ttttaagtaa agatcctaaa actggaaaac ctttactggt
    21601 aacttatgac ttatgtcgca gagcttattc ttaattaagt aagttaatat gataaaaagg
    21661 acaattaaca tgacaaatag agagaaccca acgccattga agtttttatc ctatattata
    21721 ggtttaagta tgatactact aatcacacta tttatttcta cattaatagg tgacgccaaa
    21781 attcaagcct ctacaattat agaggctatt tttaattata atcctagcaa tcaacagcaa
    21841 aacatcatca atgagattag gattcccaga aatatagcag cagtaattgt aggtatggcg
    21901 cttgcagttt ctggtgcgat tatacaaggt gttactcgta atggtcttgc tgatccggcg
    21961 ctcattggtt taaattcagg tgcttcattt gctttagcat taacatatgc agttttacca
    22021 aacacttcat ttttaatatt gatgtttgct ggatttttag gtgctattct aggaggtgct
    22081 attgtattaa tgataggccg atctagacgt gatggattta atccgatgcg tattatttta
    22141 gcgggtgcag cagtaagtgc tatgttaaca gcgctaagtc aaggtattgc attagctttt
    22201 agactaaatc aaacagtaac attttggact gctggaggcg tttcaggcac aacatggtca
    22261 caccttaagt gggcaattcc attaattggt attgcgttat tcattatatt aacaattagt
    22321 aaacaactta ccattttaaa tcttggtgaa tcattagcta aaggtttagg tcaaaatgta
    22381 acaatgatca gaggcatatg tttaattatt gctatgattc tagcaggtat tgcagttgct
    22441 atcgctggac aagttgcatt tgtaggtttg atggtacctc atatagcaag atttttaatt
    22501 ggaactgatt atgctaaaat tctaccatta acagccttgt taggtgggat actcgtgctt
    22561 gttgccgatg tgatagcacg atatttagga gaagcgcctg ttggtgcaat catttcattt
    22621 atcggtgttc cttacttttt atatttagtt aaaaaaggag gacgctcaat atgattagtt
    22681 caaataataa acgcagacaa ttgatagcac tggctgtttt tagcattcta ctatttctag
    22741 gttgtacttg gagtattacc tcaggtgaat acaacatacc tgttgaaaga tttttcaaaa
    22801 ctttaattgg acaaggtgat gccattgatg agttaatctt attagatttc aggttacctc
    22861 ggatgatgat tactattttg gctggcgcag cgcttagtat tagtggtgca atagtgcaaa
    22921 gtgtcacaaa aaatccaata gctgaaccag gtatattagg tattaacgca ggtggcggat
    22981 ttgcaatcgc attatttatt gcaattggta aaattaatgc tgacaacttt gtttatgtac
    23041 tgccgttaat aagtatacta ggtggtatca ccactgcatt gattattttt attttcagtt
    23101 ttaataaaaa tgaaggtgtt acacctgcga gtatggtatt aataggtgta ggtttacaaa
    23161 cagcattata tggtggctca attacaatta tgtcaaaatt tgatgataag caatctgatt
    23221 tcatcgctgc ttggtttgca ggtaatattt ggggtgacga atggccattt gtcattgcat
    23281 ttttaccgtg ggtgttgatt attattcctt acttactatt taaatcgaat acactaaata
    23341 ttattcatac gggtgataat attgcacgag gtctaggtgt aaggttaagc agagaacgtt
    23401 taatattatt ctttatcgca gtgatgttat catctgctgc tgtagcagta gcaggttcga
    23461 tttcgtttat cggattaatg ggtccgcata ttgccaaacg tatcgttgga ccacgtcacc
    23521 agttgttttt accaattgcc attttagtag gggcatgttt acttgttata gctgatacaa
    23581 ttggcaaaat tgtattacaa ccaggtgggg ttccagcagg tattgtcgta gcaattattg
    23641 gtgcaccgta tttcttatat ttaatgtaca aaacgaaaaa tgtatagtgt caaaggacac
    23701 aacttattgc tatgaaaggc actttattat aaggcttttc atagcatttt ttatttaatg
    23761 agccactcaa gactatttat tttttcaata atgaaccatt aagttatcaa gaggatctta
    23821 tcaaaaatat atttgataac ggtatcaggt taattcttta tgatagcgca ttcatttatt
    23881 ctgttttata ctatgactga taataccaag gaggtacaac atgatgaaaa agttaatcaa
    23941 taaaaaagaa acatttttaa ctgatatgct tgaaggattg ttaattgcgc acccagagtt
    24001 agatctgatt gctaatacag ttattgtaaa aaaagctaag aaagaacatg gtgtagcaat
    24061 agtctctgga ggtggaagcg gacatgaacc tgcgcatgcc ggttttgttg cagaaggtat
    24121 gctagatgca gcggtttgtg gcgaagtatt tacatcacct acacctgata aaatattaga
    24181 agctattaaa gcagtagata ctggtgatgg tgtattacta gttgtaaaaa actatgcagg
    24241 tgacgtgatg aatttcgaaa tggcacaaga gcttgcagaa atggaaggta taaatgttca
    24301 aactgttatt gttcgtgacg acattgctgt gacaaacgaa gtacaacgtc gtggtgttgc
    24361 aggaacagtg tttgttcata agcttgccgg ttatcttgct gaaaaaggtt attcattaac
    24421 agagataaaa tcacgtgtag aagcgttgtt acctgaaatt aaaagtattg gtatggcaat
    24481 tgagccaccg cttgttccaa ctactggaaa atatggcttt gatattgaag acgacaaaat
    24541 ggaaatcggt attggtatac atggtgaaaa aggtattcat agggaagaag taaaggatat
    24601 tgatcatatt gttggaacat tgttagacga attgtataaa gaagttactg ccaatgatgt
    24661 catattaatg gtaaatggta tgggtggtac gccgttatct gaattaaata tcgtaactaa
    24721 atatattcaa caaaatttag ctgcaagaac ggttaatgtt gctaaatggt ttgttggtga
    24781 ttatatgaca tctttagaca tgcaaggttt ttctataact atcgtgccta ataaaccaga
    24841 atatttggaa gcatttttag caccaacaac aagtcaatac tttaaataag aagtgaatat
    24901 gaataaatat acatttatga ggtggcacaa atgaaagtga atgatatgaa agcacgttta
    24961 ttaaatttag aagaaacgtt taaaaaacat gaatctgaat taactgaatt agatcgagca
    25021 attggtgatg gtgaccatgg ggttaacatg gttcgtgggt ttagtagtct taaagacaaa
    25081 cttgatgata gctcaatgca atcattgttc aaatcgactg gtacggcatt gatgtcaaat
    25141 gttgggggtg catcaggacc actgtatggc tttagctttg ttaaaatgtc tgcagtcacc
    25201 aaagatgata tggataatca agatttcatt acactaattc aggcatttgc cgaagcggtt
    25261 gaatcacgtg gtaaagttac tttaaatgaa aagacaatgt atgatgtagt agcgcgagca
    25321 gcagaggagc ttaaaaatag tgaaacttta acattcaatg atttacagca attagcagat
    25381 aatacaaaag atatggtagc aacgaaaggt agagctgcat attttggaga agaatcaaaa
    25441 ggttatattg atccaggtgc tcaaagtatg gtttatattt taaacgcttt gattggagat
    25501 gaagataatg cctaaaatta tacttgttag ccacagtaaa gaaattgcaa gtggtacaaa
    25561 atctttgtta aagcaaatgg caggtgacgt tgatattata ccaatcgggg gattaccaga
    25621 tggttcaatt ggaacttcat ttgatatcat ccaagaagtt ttgactaaat tagaggatga
    25681 tgcattgtgt ttttacgata ttggatcttc agaaatgaat gtagatatgg caattgaaat
    25741 gtatgatggt aatcatcgtg tgttaaaagt tgatgcacca attgttgaag gcagttttat
    25801 cgcagcagta aagctatcaa tcggcggttc aattgatgat gcattagcag aaatcaaaca
    25861 atcattttag ttaaaattta ctaataatga aaaatgtaaa cctttttcaa atgaa
//
LOCUS       DAHHYJ010000019        25285 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000019 DAHHYJ010000000
VERSION     DAHHYJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.019"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            122..322
                     /locus_tag="NBK22_000617"
     CDS             122..322
                     /locus_tag="NBK22_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCD2413823.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLEEYRIELKRMNNDIKK"
     gene            350..766
                     /locus_tag="NBK22_000618"
     CDS             350..766
                     /locus_tag="NBK22_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2413824.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            998..1297
                     /locus_tag="NBK22_000619"
     CDS             998..1297
                     /locus_tag="NBK22_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD2413825.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKRKSLDVDHILSLEHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            1427..1771
                     /locus_tag="NBK22_000620"
     CDS             1427..1771
                     /locus_tag="NBK22_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413826.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            1768..3429
                     /locus_tag="NBK22_000621"
     CDS             1768..3429
                     /locus_tag="NBK22_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD2413827.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQTHIYSRNDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIVSNSEEQAKTSFDEIRNVLMENKRNKTGKTPKAPYE
                     VSQTKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKVKPDGNK
                     EYIKKDENRRKTDGFMAFVHALYRPDDIVDKDMSKALDALMSIDF"
     gene            3445..4632
                     /locus_tag="NBK22_000622"
     CDS             3445..4632
                     /locus_tag="NBK22_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD2413828.1"
                     /translation="MSILEKIFKTRKDITYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DNKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLITKNYEKANSGEDDEKEKDENTLKGGDEDE
                     SGD"
     gene            4616..5353
                     /locus_tag="NBK22_000623"
     CDS             4616..5353
                     /locus_tag="NBK22_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD2413829.1"
                     /translation="MKAEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELVEMMARETWLNADEA
                     IEQGFADSKMFENDNMQIVASNTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKVSANGFSRFLF"
     gene            5377..6522
                     /locus_tag="NBK22_000624"
     CDS             5377..6522
                     /locus_tag="NBK22_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD2413830.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQSLSANQRSFFMDINKNVNYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     EGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEGTEETL"
     gene            6542..6826
                     /locus_tag="NBK22_000625"
     CDS             6542..6826
                     /locus_tag="NBK22_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413831.1"
                     /translation="MVKFKVVRAFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIVDLLNGEDNDD"
     gene            6816..7100
                     /locus_tag="NBK22_000626"
     CDS             6816..7100
                     /locus_tag="NBK22_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCD2413832.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            7084..7446
                     /locus_tag="NBK22_000627"
     CDS             7084..7446
                     /locus_tag="NBK22_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCD2413833.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            7443..7847
                     /locus_tag="NBK22_000628"
     CDS             7443..7847
                     /locus_tag="NBK22_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCD2413834.1"
                     /translation="MSVKVIGDKALERELEKRFGIKEMVKVQDKALIAGAKVIVEEVK
                     KQLKPSKDTGALINEVSFSKPEWINGKRTITVHWRGSKDRYKIVHLIEYGHVQKGTGK
                     FIKPKAMGGVNRAIRQGQNKYFETLKRELKKL"
     gene            7844..8251
                     /locus_tag="NBK22_000629"
     CDS             7844..8251
                     /locus_tag="NBK22_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413835.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            8252..8896
                     /locus_tag="NBK22_000630"
     CDS             8252..8896
                     /locus_tag="NBK22_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD2413836.1"
                     /translation="MAVKHASAPKAYINITGLGFAKLTKEGAELKYSDITKTRGLQKI
                     GVETGGELKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEDYDEDGVYEEK
                     QGKQNNYVAVWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLVDNKKSVRKYIFDSANMTNHDGDGEKGEEAFLKKILGEEYTGNVTEGNEETL"
     gene            8938..9162
                     /locus_tag="NBK22_000631"
     CDS             8938..9162
                     /locus_tag="NBK22_000631"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCD2413837.1"
                     /translation="MPDSIKTLKVGDTYDLNVVVEPSNQSKLLKYTTDQTNIVSINSD
                     GQVTAEAQGIATVKATVGNMSDTITINVEA"
     gene            9212..9562
                     /locus_tag="NBK22_000632"
     CDS             9212..9562
                     /locus_tag="NBK22_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413838.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQG
                     QQTEETRNFIQNMK"
     gene            9807..14351
                     /locus_tag="NBK22_000633"
     CDS             9807..14351
                     /locus_tag="NBK22_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD2413839.1"
                     /translation="MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNF
                     KYTEKSTHSYKQRIKELDGTITGYKKNVDDLAKQYGKVSQEQGENSAEAQKLRQEYNK
                     QANELNFLEKELEKTTTEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVY
                     GNFPEDAETVGGVLGEVNTRLGFTGKELESATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEADEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKDPREEFKKTLAEIERTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSVAVDWFSSLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTVGNAVTVLAPLLASIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNGAINSVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKAIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGALNIILGFIKFFSSLFTGNWRGVWDGIVMILKGTVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLISGIWGVVKGIFTKSLSAIWNATKSIFGFLYNSVKS
                     IFTNMKNWLSNAWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWN
                     SIKDNTVGIAGRLWDKVRNIFGNMRDGLKSIIGKIKDHIGGMVDAIKKGLNKLIEGLN
                     WVGGKLGMDEIPRLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMI
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFHFGTTMWKDIKSSASS
                     AFNWTKDQIGKGTKWLGDKVGDVMDFIDNPGKLLNYVLKAFGVDFSSLTKGMGIVGDI
                     TKASWNKIKSKAINWIKEGLESQAGDGSVFDSFRILQPYSAPPKPPNPNYPFNGGVHH
                     GVDYDTPTGTPIRTPMGGRVRSWYDNYGGGKAITVQKGRTFLWFMHLSEQLRRTGEQI
                     KAGQLIGKSGNTGSMTNYRHLNFQVNQGGESNRYSTDPIPWLRKNDKTGGKNSPGGSG
                     SENARRAIRTAQNILGGQYKASWITHEMMRVARRESNYTANAVNNWDSNARAGTPSRG
                     MFQMIDPSFRAYAKSGYNNPLNPTHQAISAMRYIVGKWVPRTGSWRAAFKRAGDYAYA
                     TGGKVYNGLYHLGEEGYPEWVIPTDPARKNEAMKMLHYAAAEVRGRKASKNKRPSQLS
                     SVNGFDDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIE
                     KRERQESTKVKFRKGGIAIQ"
     gene            14348..15832
                     /locus_tag="NBK22_000634"
     CDS             14348..15832
                     /locus_tag="NBK22_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCD2413840.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGAIYK
                     GRRLESYSFDIPLVVRNDYLSHNGIKTYDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFTIPVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENVFDTTVKWQDRYL"
     gene            15848..19633
                     /locus_tag="NBK22_000635"
     CDS             15848..19633
                     /locus_tag="NBK22_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413841.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTMSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPTRYIWGIYEPQSDDQNMNETRLRSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVGAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNIIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDMLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTQALFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDDLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNKYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            19623..19775
                     /locus_tag="NBK22_000636"
     CDS             19623..19775
                     /locus_tag="NBK22_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413842.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            19822..20109
                     /locus_tag="NBK22_000637"
     CDS             19822..20109
                     /locus_tag="NBK22_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413843.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            20167..20463
                     /locus_tag="NBK22_000638"
     CDS             20167..20463
                     /locus_tag="NBK22_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCD2413844.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            20613..20789
                     /locus_tag="NBK22_000639"
     CDS             20613..20789
                     /locus_tag="NBK22_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCD2413845.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(20842..20949)
                     /locus_tag="NBK22_000640"
     CDS             complement(20842..20949)
                     /locus_tag="NBK22_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413846.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            21001..21255
                     /locus_tag="NBK22_000641"
     CDS             21001..21255
                     /locus_tag="NBK22_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD2413847.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            21267..22022
                     /locus_tag="NBK22_000642"
     CDS             21267..22022
                     /locus_tag="NBK22_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD2413848.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNHNEWIWHERLIVKEVF"
     gene            22213..22704
                     /gene="sak"
                     /locus_tag="NBK22_000643"
     CDS             22213..22704
                     /gene="sak"
                     /locus_tag="NBK22_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCD2413849.1"
                     /translation="MLKRSLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKVII
                     EKK"
     gene            23354..23689
                     /locus_tag="NBK22_000644"
                     /pseudo
     CDS             23354..23689
                     /locus_tag="NBK22_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(23784..24233)
                     /locus_tag="NBK22_000645"
     CDS             complement(23784..24233)
                     /locus_tag="NBK22_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCD2413850.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
     gene            24917..25267
                     /gene="scn"
                     /locus_tag="NBK22_000646"
     CDS             24917..25267
                     /gene="scn"
                     /locus_tag="NBK22_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCD2413851.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGLKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT         9309 a         3469 c         5071 g         7436 t
ORIGIN      
        1 taaacgatta taatctttat cttgataaat ttgaagaaca gtttagtcaa aagtttaaaa
       61 taaacagaac ttcgataaaa gaaagaatta ttattaattt aaacaagagg agatttaaat
      121 gatgtggatt actatgacta ttgtatttgc tatattgcta ttagtttgta tcagtattaa
      181 tagtgatcgt gcaagagaga tacaagcact cagatatatg aatgattatc tacttgatga
      241 agtagttaaa actaaaggat acaacgggtt agaagaatac aggattgaat tgaagcgaat
      301 gaataacgat attaaaaagt aatttatatt atcggaggta ttgcattgaa tgataaagat
      361 tgagaaacac gatatcaaaa agcttgaaga atacattcag cacatcgata actatcgaag
      421 agagttgaag atgcgagaat atgaattact tgaaagtcat gaaccagata atgcgggagc
      481 tggcaaaagt aatttgccgg gtaacccgat tgaacgatgt gcaataaaga agtttagtga
      541 taacaggtac aatacattaa gaaatatagt taacggtgta gatagattga tagatgaaag
      601 tgatgaggat acgcttgagt tattaaggtt tagatattgg gattgtccta ttggttgtta
      661 tgaatgggaa gatatagcac attactttgg tacaagtaag acaagtatat tacgtagaag
      721 gaatgcactg atcgataagt tagcaaagta tattggttat gtgtagcgga cttttaccct
      781 atgtaagtcc gcattaaaac agtttattat gttagtatca gattaatatt taaagttatt
      841 aaatgctaat acgacgcatg aacaagaggc gcatcactat gtgatgtgtc tttttattta
      901 tgaggtatga acatgttcaa actaattgta aatacattac tacacatcaa gtatagatgc
      961 gtcttgatac tacttaagtt atataaggtg aaacattatg atgactaaag acgaacgtat
     1021 acgattctat aagtctaaag aatggcaaac aacaagaaaa agagttctag aaagagataa
     1081 ttatgaatgt caacaatgta agcgagacgg caagttaacg acatatgaca aaagcaagcg
     1141 taagtcgttg gatgtagatc atatattatc gctagaacat catccggagt ttgctcatga
     1201 cttaaacaat ttagaaacac tgtgtattaa atgtcacaac aaaaaagaaa agagatttat
     1261 aaaaaaagaa aataaatgga aagacgaaaa atggtaaata cccccgggtc aaaaaaatca
     1321 aaagcgatca aaatacttgg ggaacgggga ggggctcgac ttcgcgataa ttttaaaaat
     1381 ccatgtataa cccccctctt ataaccattt taaggcaggt gatgaaatgg agattatagt
     1441 tgatgaaaac ttagtgctta aagaaaaaga aaggctgcaa gtattatata aagacatacc
     1501 tagcaataaa ttaaaagtag ttgatggttt aattattcaa gcagcaaggc tacgtgtaat
     1561 gcttgattac atgtgggaag acataaaaga aaaaggtgac tatgatttat ttactcaatc
     1621 tgaaaaggcg ccaccatatg aaagagaaag accagtagcc aagctattta atgctagaga
     1681 tgctgcatat caaaaaataa tcaaacaatt atcggattta ttgcccgaag agaaagaaga
     1741 cacagaaaca ccatctgatg attacctatg attagtaata aatacgttga tgaatatata
     1801 aatttgtgga aacaaggaaa gataatttta aataaagaaa gaattgattt atttaattat
     1861 ctacaaacgc atatatattc acgaaatgat gtatattttg atgaacagaa aatcgaggat
     1921 tgtatcaaat ttattgaaaa atggtatttt ccaacattac catttcaaag gtttatcata
     1981 gctaatatat ttcttataga taaaaataca gatgaagctt tctttacaga atttgctatt
     2041 ttcatgggac gtggaggcgg gaaaaacggt ctaataagtg caattagtga ttttctttct
     2101 acgcccctac atggagttaa agaatatcac atctctattg tttctaatag tgaagaacaa
     2161 gcaaaaacat cgtttgatga aatcagaaac gttttaatgg aaaacaaacg aaataagacg
     2221 ggtaaaacgc caaaagctcc ttatgaagtt agtcaaacaa aaataataaa ccgtgcaact
     2281 aaatcagtta ttcgatataa cacatcaaac acaaaaacca aagacggtgg acgtgagggg
     2341 tgtgttattt ttgatgaaat tcattatttc tttggtcctg aaatggtaaa cgttaaacgt
     2401 ggtggactag gtaaaaagaa aaatagaaga acgttttata taagtactga tggttttgtt
     2461 agagagggtt atatcgatgc aatgaagcac aaaattgcaa gtgtattaag tggcaaggtt
     2521 aaaaatagta gattgtttgc tttttattgt aagttagacg atccaaaaga agttgatgac
     2581 agacagacgt gggaaaaggc gaacccaatg ttacataaac cgttatcaga atacgctaaa
     2641 acactgctaa gtacgattga agaagaatat aacgatttac cattcaaccg ttcaaataag
     2701 cccgaattca tgactaagcg aatgaatttg cctgaagttg accttgaaaa agtcatagca
     2761 ccatggaaag aaatactagc gactaataga gagataccaa atttagataa tcaaatgtgt
     2821 attggtggtt tagactttgc aaacattcga gattttgcaa gtgtagggct attattccga
     2881 aaaaacgatg attacatttg gttaggacat tcgtttgtaa gacaagggtt tttggatgat
     2941 gtcaaattag aacctcctat taaagaatgg gaaaaaatgg gattattgac cattgtcgat
     3001 gatgatgtca ttgaaattga atatatagtt gattggtttt taaaggctag agaaaaatat
     3061 gggcttgaaa aagtcatagc tgataattat agaactgata ttgtaagacg tgcgtttgag
     3121 gatgctggca taaaacttga agtacttaga aatccaaaag caatacatgg attacttgca
     3181 ccacgtatcg atacaatgtt tgcgaaacat aacgtaatat atggagacaa tcctttgatg
     3241 cgttggttta ctaataatgt tgcagtaaag gttaaacccg atggtaataa agaatatatt
     3301 aaaaaagatg aaaatagaag aaaaaccgat gggttcatgg cttttgttca cgcattatat
     3361 agaccagacg atatagtaga caaagatatg tctaaagcgc ttgatgcatt aatgagtata
     3421 gatttctaat agaggaggtg agacatgagt attctagaaa agatatttaa aactaggaaa
     3481 gatataacat atatgcttga tttagatatg atagaagatc tatcacaaca agcgtatgtg
     3541 aaacgtttag cgattgatag ttgtattgaa tttgttgcgc gagctgtcgc tcaaagtcat
     3601 tttaaagtat tggaaggtaa tagaattcaa aagaatgatg tttactacaa gttaaatata
     3661 aaaccaaata ctgacttatc aagcgatagt ttttggcaac aagttatata taaactaatt
     3721 tatgataacg aggttttaat cgtagtaagt gacaacaaag aattacttat cgcagatagc
     3781 ttttacagag aagagtacgc tttgtatgat gatatattca aagatgtaac ggttaaagat
     3841 tatacttatc aacgtacttt cacaatgcaa gaggtcatat atttaaagta caacaacaat
     3901 aaagtgacac actttgtaga aagtctattc gaagattacg ggaaaatatt cggaagaatg
     3961 ataggtgcac aattaaaaaa ctatcaaata agagggattt tgaaatctgc ctctagcgca
     4021 tatgacgaaa agaatataga aaaattacaa gcgttcacaa ataaattatt caatactttt
     4081 aataaaaatc aactagcaat cgcgcctttg atagaaggtt ttgattatga ggaattatct
     4141 aatggtggta agaatagtaa catgcctttt tctgaattga gtgagctaat gagagatgca
     4201 ataaaaaatg ttgcgttgat gattggtata cctccaggtt tgatttacgg agaaacagct
     4261 gatttggaaa aaaacacgct tgtatttgag aagttctgtt taacaccttt attaaaaaag
     4321 attcagaacg aattaaacgc gaaactcata acacaaagca tgtatttgaa agatacaaga
     4381 atagaaattg tcggtgtgaa taaaaaagac ccacttcaat atgctgaagc aattgacaaa
     4441 cttgtaagtt ctggttcatt tacaaggaat gaggtgcgga ttatgttagg tgaagaacca
     4501 tcagacaatc ctgaattaga cgaatacctg attactaaaa actacgaaaa agctaacagt
     4561 ggtgaagatg atgaaaaaga aaaagatgaa aacactttga aaggtggtga tgaagatgaa
     4621 agcggagatt aaaggcgtca tcgtttccaa cgaagataaa tgggtttacg aaatgcttgg
     4681 tatggattcg acttgtccta aagatgtttt aacacaacta gaatttagtg atgaagatgt
     4741 tgatattata attaactcaa atggtggtaa cctagtagct ggtagtgaaa tatatacaca
     4801 tttaagagct cataaaggca aagtgaatgt tcgtatcaca gcaatagcag caagtgcggc
     4861 atcgcttatc gcaatggctg gtgaccacat cgaaatgagt ccggttgcta gaatgatgat
     4921 tcacaatcct tcaagtattg cgcaaggaga agcgaaagat ctaaatcatg ctgcagaaac
     4981 attagaacat gttggtcaaa taatggctga ggcatatgcg gttagagctg gtaaaaacaa
     5041 acaagaactt gtagaaatga tggctaggga aacgtggcta aatgctgatg aagccattga
     5101 acaaggtttt gcggatagta aaatgtttga aaacgacaat atgcaaattg tagcaagcaa
     5161 tacacaagtg ttatcgaaag atgtattaaa tcgtgtaaca gctttggtaa gtaaaacgcc
     5221 agaggttaac attgatattg acgcaatagc aaataaagta attgaaaaaa taaatatgaa
     5281 agaaaaggaa tcagaaatcg atgttgcaga tagtaaagta tcagcaaatg gattttcaag
     5341 attccttttt taatacaaaa aataggaggt cataaaatga ctataaattt atcggaaaca
     5401 ttcgcaaatg cgaaaaacga atttattaat gcagtaaaca acggtgaacc gcaagaaaga
     5461 caaaatgaat tgtacggtga catgattaac caactatttg aagaaactaa attacaagca
     5521 aaagcagaag ctgaaagagt ttctagttta cctaaatcag cacaatcttt gagtgcaaac
     5581 caaagaagtt tcttcatgga tatcaataaa aacgttaact ataaagaaga aaaacttttg
     5641 ccagaagaaa caattgatag aatttttgaa gatttgacga cgaatcatcc gttattagct
     5701 gatttaggta ttaaaaacgc tggtttgcgt ttgaagttct taaaatctga aacttctggc
     5761 gtagccgttt ggggtaaaat ctatggtgaa attaaaggtc aattagatgc tgcgttcagt
     5821 gaagaaacag caattcaaaa taaattgaca gcgtttgttg ttttaccaaa agatttaaat
     5881 gattttggtc ctgcgtggat tgaaagattt gttcgtgttc aaatcgaaga agcatttgca
     5941 gtggcgcttg aaactgcgtt cttaaaaggt actggtaaag accaaccaat cggcttaaac
     6001 cgtcaagtac aaaaaggtgt atcggtaact gagggtgctt atccagagaa agaagaacaa
     6061 ggtacgctta catttgctaa tccgcgcgct acggttaatg aattgacgca agtgtttaaa
     6121 taccactcaa ctaacgagaa aggtaaatca gtagcggtta aaggtaatgt aacaatggtt
     6181 gttaatccgt ccgatgcttt tgaggttcaa gcacagtata cacatttaaa tgcaaatggc
     6241 gtatatgtta ctgctttacc atttaatttg aatgttatcg agtctacagt ccaagaagca
     6301 ggtaaggttt taacgtacgt taaaggttta tatgatggtt atttagctgg tggtattaat
     6361 gttcagaaat ttaaagaaac acttgcgtta gatgatatgg atttatacac tgcaaaacaa
     6421 tttgcttacg gcaaagcgaa agataataaa gttgctgctg tttggaaatt agatttaaaa
     6481 ggacataagc cagctttaga aggtaccgaa gaaacactat aaaattttat gaggtgataa
     6541 aatggtgaaa tttaaagttg ttagagcttt taaagacata gagcacaatc aacacaagta
     6601 caaagtaggg gagttgtatc cagctgaagg gtataacaat cctcgtgttg aattgttgac
     6661 aaatcaaatc aaaaataagt acgacaaagt ttatatcgta cctttagata agctgacaaa
     6721 acaagaatta ttagaactat gcgaatcatt acaaaaaaaa gcgtctagtt caatggttaa
     6781 aagtgaaatc gtcgacttat tgaatggtga agacaatgac gattgatgat ttgcttgtca
     6841 aatttaaatc acttgaaaag attgaccata attcagagga tgagtactta aagcagttgt
     6901 taaaaatgtc gtacgagcgt ataaaaaatc agtgcggagt ttttgaatta gagaatttaa
     6961 taggtcaaga attgatactt atacgcgcta gatatgctta tcaagattta ttagaacact
     7021 tcaacgataa ttacagacct gaaataatag atttttcgtt atctctaatg gaggtatcag
     7081 aagatgaaga aagtgtttaa aaaacctaga attacaacta aacgtttaaa tactcgtgtt
     7141 catttttata agtatactga aaataatggt ccagaagctg gagaaaaaga agaaaaatta
     7201 ttatatagct gttgggcgag tattgatggt gtctggttac gtgaattaga acaagctatc
     7261 tcaaacggaa cccaaaatga cattaaattg tatattcgtg atccgcaagg tgattattta
     7321 cccagtgaag aacattatct tgaaattgaa tcaagatatt tcaaaaatcg tttgaatata
     7381 aagcaagtat caccagattt ggataataaa gactttatta tgattcgtgg aggatatagt
     7441 tcatgagtgt gaaagtgata ggtgataaag cattagaaag agaattagaa aaacgttttg
     7501 gcataaaaga gatggtaaaa gttcaagata aggcgttaat agctggtgct aaggtaattg
     7561 ttgaagaagt aaaaaaacaa ctaaagccct caaaagatac gggagcatta attaatgagg
     7621 taagttttag taaacctgaa tggataaacg gaaaacgtac aattactgtt cattggcgag
     7681 gttctaaaga ccgttataaa atcgtacatt taattgaata tggacacgtt caaaaaggaa
     7741 caggtaaatt tatcaaacct aaagctatgg gcggtgttaa tagagcaata agacaagggc
     7801 aaaataagta ttttgagacg ctaaaaaggg agttgaaaaa attgtgattg atattttgta
     7861 caaagttcat gaagtgatta gtcaagacag aattattaga gagcacgtaa atatcaataa
     7921 tattaagttc aataaatacc ctaatgtaaa agatactgat gtacctttta ttgttattga
     7981 cgatatcgac gacccaatac ctacaactta tactgacgga gatgagtgtg catatagtta
     8041 tattgtccaa atagatgttt ttgttaagta caatgatgaa tataatgcga gaatcataag
     8101 aaataagata tctaatcgca ttcaaaagtt attatggtct gaactaaaaa tgggaaatgt
     8161 ttcaaatgga aaaccggaat atatagaaga atttaaaaca tatagaagct ctcgcgttta
     8221 cgagggcatt ttttataagg aggaaaatta aatggcagta aaacatgcaa gtgcgccaaa
     8281 ggcgtatatt aacattactg gtttaggttt cgctaaatta acgaaagaag gcgcggaatt
     8341 aaaatatagt gatattacaa aaacaagagg attacaaaaa attggtgttg aaactggtgg
     8401 agaactaaaa acagcttatg ctgatggcgg tccaattgaa tcagggaata cagacggaga
     8461 aggtaaaatc tcattacaaa tgcatgcgtt ccctaaagag attcgcaaaa ttgtttttaa
     8521 tgaagattat gatgaagatg gcgtttacga agagaaacaa ggtaaacaaa acaattacgt
     8581 agctgtatgg ttcagacaag agcgtagaga cggtacattt agaacagttt tattacctaa
     8641 agttatgttt acaaatccta aaatcgatgg agaaacggct gagaaagatt gggatttctc
     8701 aagtgaagag gttgaaggtg aggcactttt ccctttagtt gataataaaa agtcagtacg
     8761 taagtatatc tttgattcag ctaacatgac aaatcatgat ggagacggtg aaaaaggcga
     8821 agaggctttc ttaaagaaaa ttttaggcga agaatatact ggaaacgtga cagagggtaa
     8881 cgaagaaact ttgtaacaaa accggcttca tcggaaactg cggtaaagtc ggttaatata
     8941 ccagatagca ttaaaacact taaagttggc gacacatacg atttaaatgt tgtagtagag
     9001 ccatctaatc aaagtaagtt attgaaatac acaacagatc aaacgaatat tgtatcaatc
     9061 aatagtgatg gtcaagttac tgcggaagca caaggcattg ctacggttaa agcaacagtt
     9121 ggtaatatga gtgacactat aacaataaat gtagaagcat aagagggggc aacccctcta
     9181 ttttatttga aaataaggag agtattataa aatggcaaaa ttaaaacgta acattattca
     9241 attagtagaa gacccgaaag caaatgaaat taaattacaa acgtacttaa caccacactt
     9301 catttcattt gaaattgtat acgaagcaat ggatttaatc gatgatattg aggacgaaaa
     9361 tagcacgatg aaaccaagag aaatcgctga cagattgatg gatatggttg taaaaattta
     9421 cgataaccaa ttcacagtta aagacctaaa agaacgtatg catgcacctg atggaatgaa
     9481 tgcacttcgt gaacaagtga ttttcattac tcaaggtcag caaactgagg aaactagaaa
     9541 ttttatccag aacatgaaat aaagcctgaa gatttaacat ataaagcaat gttgaaaaat
     9601 atggatactc tcatgatgga cttaattgaa aatggtaaag acgctaacga agttttaaaa
     9661 atgccatttc attatgtact ttccatatat caaaataaaa acaataacat ttctgaagaa
     9721 aaagcagagg ctttaattga tgcgttttaa ccttaaccgt ttggttaggg ttattttttt
     9781 gaactttttt agaaaggagg taaaaaatgg gagaaagaat aaaaggttta tctataggtt
     9841 tggatttaga tgcagcaaat ttaaatagat catttgcaga aatcaaacga aactttaaaa
     9901 ctttaaattc tgacttaaag ttaaccggta acaacttcaa atataccgaa aaatcaactc
     9961 atagttacaa acaaaggatt aaagaacttg atggaactat cacaggttat aagaaaaacg
    10021 ttgatgattt agccaagcaa tatggcaagg tatctcaaga acagggcgaa aacagcgcgg
    10081 aagctcaaaa attacgacaa gaatataaca aacaagcaaa tgagctgaat tttttagaaa
    10141 aagaactaga aaaaacaaca actgagtttg aagagttcaa aaaagctcaa gttgaagctc
    10201 aaagaatggc agaaagtggc tggggaaaaa ccagtaaagt ttttgaaagt atgggaccta
    10261 aattaacaaa aatgggtgat ggtttaaaat ccattggtaa aggtttgatg attggtgtaa
    10321 ctgcacctgt tttaggtatt gcagcagcat caggaaaagc ttttgcagaa gttgataaag
    10381 gtttagatac agttacccaa gcaacaggag caaccggcgg agagcttaag aagttgcaga
    10441 attcatttaa agatgtttat ggcaactttc cagaagacgc tgagactgta ggcggtgttt
    10501 taggggaagt taacacaagg ttaggtttca ctggcaaaga acttgagagt gccacagagt
    10561 cattcttgaa atttagtcac ataacaggtt ctgacggcgt acaagccgtt caattaatta
    10621 cgcgtgcaat gggtgatgca ggtattgaag ctgatgagta tcaaagtgta cttgatatgg
    10681 tagcgaaagc agcacaggct agcggtataa gtgttgatac attagctgat agcattacta
    10741 aatacggtgc tccaatgagg gctatgggct ttgagatgaa agaatcaatc gctttattct
    10801 ctcaatggga gaaatcaggt gttaatactg aaatagcctt cagtggtttg aaaaaagcta
    10861 tatccaattg gggtaaagcg ggtaaagacc caagagaaga atttaagaag acattagcag
    10921 aaattgaaag gacaccggat atagctagcg caacaagttt agcgattgaa gcatttggtg
    10981 caaaagcagg tcctgattta gcagatgcta ttaaaggcgg tcgctttagt taccaagagt
    11041 tcttaaaaac tatcgaagat tcgcaaggaa cggtcaatca gacatttaaa gattctgaaa
    11101 gtggctccga aagatttaaa gtagcaatga ataaacttaa attagtaggt gctgatgtat
    11161 gggcttctat tgaaagtgcg tttgctccag tcatggaaga attaatcaaa aagctatctg
    11221 tagcagttga ttggttttca agtttaagtg atggatctaa aaggtcgatt gttatattcg
    11281 gtggtattgc tgctgcaatt ggtcctgtag tttttggatt aggtgcattc ataagcacag
    11341 ttggcaacgc agtaactgta ttagctccat tattagctag tattgcaaag gctgacggat
    11401 tgattagttt tttatcaact aaagtgccta ttttaggaac agtcttcaca gcattaactg
    11461 gtccaattgg tatcgtgtta ggtgtactgg ctggtttagc agtcgcattt acaatagctt
    11521 ataagaaatc tgaaacattc agaaattttg ttaatggtgc aattaacagt gttaaacaaa
    11581 cgtttagtaa tttcattcaa tttatccaac ctttcattga ttccgttaaa aacgtcttta
    11641 aacaagcggt ttcagcaatc gttgatttcg ctaaagatat ttggagtcaa attaatggat
    11701 tctttaatga aaacggaatt tctattgttc aagcgcttca aaatatatgc aattttatca
    11761 aagctatatt tgaatttatc ttaaattttg taattaaacc aatcatgttt gcgatttggc
    11821 aagtgatgca atttatttgg ccggcggtta aagccttgat tgtcagtact tgggagaata
    11881 taaaaggagt aatacaaggt gctttaaata tcatacttgg ctttattaag ttcttttcaa
    11941 gtttattcac tggtaattgg cgaggtgttt gggacggtat tgtgatgata ctaaaaggca
    12001 ctgtgcagtt aatttggaat ttaatacaac tgtggtttgt aggtaagatt ctaggtgttg
    12061 ttagatactt tggtggattg cttaaaggtt taatatccgg tatctggggt gttgtcaaag
    12121 gtattttcac aaaatcatta tctgcaattt ggaatgcaac gaaaagtatt tttggtttct
    12181 tatacaatag tgttaaatct attttcacta atatgaaaaa ctggttatct aatgcgtgga
    12241 ataatatcaa aagcaatacc gtcggcaagg ctcattcgtt atttacgggt gtaaggtcta
    12301 aattcacaag tttatggaat gcgacgaaag atatatttac taaattaaga aattggatgt
    12361 caaacatctg gaactctatt aaagataaca cggtaggtat agctggtcgt ttgtgggata
    12421 aagtacgtaa tatcttcgga aacatgcgtg acggtttaaa atctatcatt ggtaaaatta
    12481 aagatcatat cggcggtatg gtagatgcta ttaaaaaagg acttaataaa ttaattgaag
    12541 gcttaaactg ggtcggtggt aagttaggta tggatgaaat acctaggtta cacactggta
    12601 cagagcacac acatactact acaagattag ttaagaacgg taagattgca cgtgatacat
    12661 tcgctacagt tggggataaa ggacgtggaa atggtccaaa tggttttaga aatgaaatga
    12721 ttgaattccc taatggtaaa cgtgtaatca cacctaatac agacactact gcttatttac
    12781 ctaaaggctc aaaagtatac aacggtgcac aaacttattc aatgttaaac ggaacgcttc
    12841 cgagatttca tttcggtact actatgtgga aagatattaa atctagtgca tcatcggcat
    12901 ttaactggac aaaagatcaa ataggtaaag gtaccaaatg gcttggcgat aaagttggcg
    12961 atgtaatgga ctttattgat aatccgggta agcttttaaa ttatgtgctc aaagcgtttg
    13021 gtgttgactt tagctctcta actaaaggta tgggtattgt tggcgatata acaaaagcgt
    13081 cttggaataa gattaaaagt aaggcgatta attggataaa agaaggatta gagagccaag
    13141 cgggagatgg ttctgtgttt gatagtttca gaatactaca accttattca gcaccgccaa
    13201 aacctcctaa ccccaattat ccatttaacg gaggcgttca tcatggtgtt gactatgata
    13261 cgccgaccgg tacccctata cgtacgccaa tgggtggacg tgttagaagt tggtatgaca
    13321 actatggtgg cggtaaagca attactgttc aaaaaggtcg aacatttttg tggttcatgc
    13381 acttatctga acaattgcgt agaacaggtg aacaaattaa agctggtcaa ttaattggta
    13441 aatcaggtaa tacaggttct atgactaatt accgccattt aaatttccaa gtcaatcaag
    13501 gcggagagtc caataggtat tcgacagacc ctattccttg gttacggaaa aacgacaaaa
    13561 ctggtggaaa gaattcacct ggagggagtg gttctgaaaa tgcgcgcaga gcgattagaa
    13621 cagctcaaaa tatacttgga ggtcaataca aagctagctg gattacacac gaaatgatgc
    13681 gtgtagcaag acgtgaatcc aattatacag ctaatgcagt taataattgg gatagcaacg
    13741 caagagctgg tacaccttca agaggtatgt tccaaatgat agatccttca tttagagcgt
    13801 acgcaaagtc gggttacaat aatcctctta acccaactca tcaagctata tcggctatga
    13861 gatatattgt gggtaaatgg gtaccaagaa cgggctcatg gagagctgcg ttcaaacgcg
    13921 ctggtgatta cgcatatgct actggtggca aagtctacaa cggattgtac cacttaggag
    13981 aagaaggata tccagagtgg gttattccta ctgatcctgc acgtaaaaac gaagcaatga
    14041 agatgttaca ttatgcagca gcagaagtaa gagggagaaa agcgagtaaa aataagcgtc
    14101 ctagtcaatt gtctagtgta aatgggtttg atgacccaag cttattattg aaaatgattg
    14161 aacaacagca acaacaaata gctttattac tgaaaatagc gcaatcaaac gatgtgattg
    14221 cagataaaga ttatcagccg attattgacg aatacgcttt tgataaaaag gtgaacgcgt
    14281 ctatagaaaa gcgagaaagg caagaatcaa caaaagtaaa gtttagaaaa ggaggaattg
    14341 ctattcaatg atagacacta ttaaagtgaa caacaaaaca attccttggt tgtatgtcga
    14401 aagagggttt gaaataccct cttttaatta tgttttaaaa acagaaaatg tagatggacg
    14461 ttcgggggct atatataaag ggcgtaggct tgaatcttat agttttgata tacctttagt
    14521 ggtacgtaat gactatttat ctcacaacgg cattaaaaca tatgatgacg tcttgaatga
    14581 attagtaaag ttttttaact acgaggaaca agttaaatta caattcaaat ctaaagattg
    14641 gtactggaac gcttattttg aaggaccaat aaagctgcac aaagaattta caatacctgt
    14701 taagttcact atcaaagtag tactaacaga cccttacaaa tattcagtaa caggaaataa
    14761 aaatactgcg atttcagacc aagtttcagt tgtaaatagt gggactgctg acactccttt
    14821 aattgttgaa gcccgagcaa ttaaaccatc tagttacttt atgatcacta aaaatgatga
    14881 agattatttt atggttggtg atgatgaggt aaccaaagaa gttaaggatt acatgcctcc
    14941 tgtttatcat agtgagtttc gtgatttcaa aggttggact aagatgatta ctgaagatat
    15001 tccaagtaat gatttaggtg gtaaggtcgg cggtgacttt gtgatatcca atcttggcga
    15061 aggatataaa gcaactaatt ttcctgatgc aaaaggttgg gttggtgctg gcacgaaacg
    15121 agggctccct aaagcgatga cagattttca aattacctat aaatgtattg ttgaacaaaa
    15181 aggtaaaggt gccggaagaa cagcacaaca tatttatgat agtgatggta agttacttgc
    15241 ttctattggt tatgaaaata aatatcatga tagaaaaata ggacatattg ttgttacgtt
    15301 gtataaccaa aaaggagacc ccaaaaagat atacgactat cagaataaac cgataatgta
    15361 taacttggac agaatcgttg tttatatgcg gctcagaaga gtaggtaata aattttctat
    15421 taaaacttgg aaatttgatc acattaaaga cccagataga cgtaaaccta ttgatatgga
    15481 tgagaaagag tggatagatg gcggtaagtt ttatcagcgt ccagcttcta tcatagctat
    15541 ctatagtgcg aagtataatg gttataagtg gatggagatg aatggattag gttcattcaa
    15601 tacggagatt ctaccaaaac cgaaaggcgc aagggatgtc attatacaaa aaggtgattt
    15661 agtaaaaata gatatgcaag caaaaagtgt tgtcatcaat gaggaaccaa tgttgagcga
    15721 gaaatcgttt ggaagtaatt atttcaatgt tgattctggg tacagtgaat taatcataca
    15781 acctgaaaac gtctttgata cgacggttaa atggcaagat agatatttat agaaaggaga
    15841 tgagagtgtg atacatgttt tagattttaa cgacaagatt atagatttcc tttctactga
    15901 tgacccttcc ttagttagag cgattcataa acgtaatgtt aatgacaatt cagaaatgct
    15961 tgaactgctc atatcatcag aaagagctga aaagttccgt gaacgacatc gtgttattat
    16021 aagggattca aacaaacaat ggcgtgaatt tattattaac tgggttcaag atacgatgga
    16081 cggctacaca gagatagaat gtatagcgtc ttatcttgct gatataacaa cagctaaacc
    16141 gtatgcacca ggcaaatttg agaaaaagac aacttcagaa gcattgaaag atgtgttgag
    16201 cgatacaggt tgggaagttt ctgaacaaac cgaatacgat ggcttacgta ctatgtcatg
    16261 gacttcttat caaactagat atgaagtttt aaagcaatta tgtacaacct ataaaatggt
    16321 attggatttt tatatagagc ttagttctaa taccgtcaaa ggtagatatg tggtactcaa
    16381 aaagaaaaac agcttattca aaggtaaaga aattgagtat ggtaaagatt tggttgggtt
    16441 aactaggaag attgatatgt cagaaatcaa aacagcatta attgctgtgg gacctgaaaa
    16501 tgacaaagga aagcgtttag agttagttgt gactgatgac gaagcacaaa gtcaattcaa
    16561 cttacctacc cgttatattt ggggaatata cgaacctcaa tcagatgatc aaaatatgaa
    16621 tgaaacacgg ttgcgttctt tagccaaaac agagttaaat aaacgtaagt cggcagttat
    16681 gtcatatgag attacttcta ctgatttgga agttacgtat ccgcacgaga ttatatcaat
    16741 tggtgataca gtcagagtaa aacatagaga ttttaacccg ccattgtatg taggggcaga
    16801 agttattgcc gaagaatata acataatttc agaaaatagc acatatacat tcggtcaacc
    16861 taaagagttc aaagaatcag aattacgaga agagtttaac aagcgattga acataataca
    16921 tcaaaagtta aacgataata ttagcaatat caacactata gttaaagatg ttgtagatgg
    16981 tgaattagaa tactttgaac gcaaaataca caaaagtgat acaccgccag aaaatccagt
    17041 caatgatatg ctttggtatg atacaagtaa ccctgatgtt gctgtcttgc gtagatattg
    17101 gaatggtcga tggattgaag caacaccaaa tgatgttgaa aaattaggtg gtataacaag
    17161 agagaaagcg ctattcagtg aattaaacaa tatttttatt aatttatcta tacaacacgc
    17221 tagtcttttg tcagaagcta cagaattact gaatagcgag tacttagtag ataatgattt
    17281 gaaagcggac ttacaagcaa gtttagacgc tgtgattgat gtttataatc aaattaaaaa
    17341 taatttagaa tctatgacac ctgaaactgc aacgattggt cggttggtag atacacaagc
    17401 tttatttctt gagtatagaa agaaattaca agatgtttat acagatgtag aagatgttaa
    17461 aatcgccatt tcagatagat ttaaattatt acagtcacaa tacactgatg aaaaatataa
    17521 agaagcgttg gaaataatag caacaaaatt tggtttaacg gtgaatgaag atttgcagtt
    17581 agtcggagaa cctaatgttg ttaaatcagc tattgaagca gctagagaat ccacaaaaga
    17641 acaattacgt gactatgtaa aaacatcgga ctataaaaca gacaaagacg gtattgttga
    17701 acgtttagat actgctgaag ctgagagaac gactttaaaa ggtgaaatca aagataaagt
    17761 tacgttaaac gaatatcgaa acggattgga agaacaaaaa caatatactg atgaccagtt
    17821 aagtgatttg tccaataatc ctgagattaa agcaagtatt gaacaagcaa atcaagaagc
    17881 gcaagaagct ttaaaatcat acattgatgc tcaagatgat cttaaagaga aggaatcgca
    17941 agcgtatgct gatggtaaaa tttcggaaga agagcaacgc gctatacaag atgctcaagc
    18001 taaacttgaa gaggcaaaac aaaacgcaga actaaaggct agaaacgctg aaaagaaagc
    18061 taatgcttat acagacaaca aggtcaaaga aagcacagat gcacagagga gaacactgac
    18121 tcgctatggt tctcaaatta tacaaaatgg taaggaaatc aaattaagaa ctactaaaga
    18181 agagtttaat gcaaccaatc gtacactttc aaatatatta aacgagattg tccaaaacgt
    18241 tacagatgga acaacaatca gatatgatga taacggagtg gctcaagctt taaatgtggg
    18301 gccacgtggt attagattaa atgctgataa aattgatatt aacggtaata gagaaataaa
    18361 ccttcttatc caaaatatgc gagataaagt agataaaacc gatattgtca acagccttaa
    18421 tttatcaaga gagggtcttg atatcaatgt taatagaatt ggaattaaag gcggtaacaa
    18481 taacaaatat gttcaaatac agaatgattc tattgaacta ggtggtattg tgcaacgaac
    18541 ttggaaaggc aaacgatcaa ccgatgatat attcacacgt cttaaagatg gacatctaag
    18601 gtttagaaat aataccgcag gcggttcact ttatatgtca cattttggta tttcaacata
    18661 tattgatgga gaaggcgaag acggaggttc atccggtact attcaatggt gggataaaac
    18721 ttacagtgat agcggtatga atggcataac aatcaattcc tatggtggtg tcgttgcact
    18781 aacgtcagat aataatcggg ttgttctgga gtcttacgct tcatcgaata tcaaaagcaa
    18841 acaggcaccg gtgtatttat atccaaacac agacaaagtg cctggattaa accgatttgc
    18901 attcacgctg tctaatgcag ataatgctta ttcgagtgac ggttatatta tgtttggttc
    18961 tgatgagaac tatgattacg gtgcgggtat caggttttct aaagaaagaa ataaaggtct
    19021 tgttcaaatt gttaatggac gatatgcaac aggtggagat acaacaatcg aagcagggta
    19081 tggcaaattt aatatgctga aacgacgtga tggtaatagg tatattcata tacagagtac
    19141 agacctactg tctgtaggtt cagatgatgc aggagatagg atagcttcta actcaattta
    19201 tagacgtact tattcggccg cagctaattt gcatattact tctgctggca caattgggcg
    19261 ttcgacatca gcgcgtaaat acaagttatc tatcgaaaat caatataacg atagagatga
    19321 acaactggaa cattcaaaag ctattcttaa cttacctatt agaacgtggt ttgataaagc
    19381 tgagtctgaa attttagcta gagagctgag agaagataga aaattatcgg aagacaccta
    19441 taaacttgat agatacgtag gtttgattgc tgaagaggtg gagaatttag gattaaaaga
    19501 gtttgtcacg tatgatgaca aaggagaaat tgaaggtata gcgtatgatc gtctatggat
    19561 tcatcttatc cctgttatca aagaacaaca actaagaatc aagaaattgg aggagtcaaa
    19621 gaatgcagga taacaaacaa ggattacaag ctaatcctga atatacaatt cattatttat
    19681 cacaggaaat tatgaggtta acacaagaaa acgcgatgtt aaaagcgtat atacaagaaa
    19741 ataaagaaaa tcaacaatgt gctgaggaag agtaatcctt agcactattt ttatacaaaa
    19801 atttaaggag gtcatttaat tatggcaaaa gaaattatca acaatacaga aaggtttatt
    19861 ttagtacaaa tcgacaaaga aggtacagaa cgtgtagtat atcaagattt cacaggaagt
    19921 tttacaactt ctgaaatggt taaccatgct caagatttta aatctgaaga aaacgctaag
    19981 aaaattgcgg agacgttaaa tttgttatat caattaacta acaaaaaaca acgtgtgaaa
    20041 gtagttaaag aagtagttga aagatcagat ttatctccag aggtaacagt taacactgaa
    20101 acagtatgaa aagctatgag ttagatactc ataatcttta ttcttttaga aagcgggtgt
    20161 actgaattgg ggtggttcaa aaaacacgaa catgaatggc gcatcagaag gttagaagag
    20221 aatgataaaa caatgctcag cacactcaac gaaattaaat taggtcaaaa aacccaagag
    20281 caagttaaca ttaaattaga taaaacctta gatgctattc aaaaagaaag agaaatagat
    20341 gaaaagaata agaaagaaaa tgataagaac atacgtgata tgaaaatgtg ggtgcttggt
    20401 ttagttggga caatatttgg gtcgctaatt atagcattat tgcgtatgct tatgggcata
    20461 taagagaggt gaataaaatg tttaaactaa tctttggtta tagtttctgg acatgttttt
    20521 ggttcggtaa atgtaaatga gttttagtca gtgcttcggt actgactttt tatttattgt
    20581 tgtaattatg gtaatatgca gaagtgagca agttggatag atggtggcta tctgagtata
    20641 aggaggtggt gcctatggtg gcattactga aatctttaga aaggagacgc ctaatgatta
    20701 caattagtac catgttgcag tttggtttat tccttattgc attgataggt ctagtaatca
    20761 agcttattga attaagcaat aaaaaataac catcgctaac tttggctggt ttcgatggtt
    20821 aaatggttat taatttaatc tttaatctaa aatagccacc gtctttttaa cgggctcatt
    20881 agggtaacat gtttgcgcat gttgcccttt ttctatatat aaattaacac accataatat
    20941 aaatatcaaa tagacggctt attagtcgtc tttttatttt gggtaaaagg agataagaat
    21001 atgattaatt ggaaaattag aatgaaacaa aaatcatttt gggtagcgat attgtcagct
    21061 atctttttat ttgctcaaaa catcgcaaaa gctattgggt atgatatcca agtttataca
    21121 gagcaattaa cagacggttt aaacgctata ttaggatttt tagtattaac tggtgtgatt
    21181 caagacccga ctactaaagg tataggtgat agccaccaag ctttagaata tgaagaacca
    21241 agaagaaaat actaggaggt aaaataatga aaacatacag tgaagcaaga gcaaggttac
    21301 gttggtatca aggtagatat attgattttg acggttggta tggttaccaa tgtgcagatt
    21361 tagcagttga ttacatttat tggttgttag aaattagaat gtggggaaat gcaaaagatg
    21421 caatcaataa cgattttaaa aacatggcaa cagtatatga aaacacacca tcgtttgttc
    21481 cacaaatagg tgatgtggct gtatttacca aaggaatata taaacaatac ggtcatattg
    21541 gtttagtgtt taatggtggt aatacaaacc aatttttaat tttggaacag aactatgacg
    21601 gtaacgcaaa tacgcctgca aagttacgtt gggataatta ttacggctgt actcacttta
    21661 ttagacctaa gtataaaagt gagggcttaa tgaataagat cacaaataaa gttaaaccac
    21721 ctgctcaaaa agcagtcggt aaatctgcaa gtaaaataac agttggaagt aaagcgcctt
    21781 ataaccttaa atggtcaaaa ggtgcttatt ttaatgcgaa aatcgacggc ttaggtgcta
    21841 cttcagccac tagatacggt gataatcgta ctaactatag attcgatgtt ggacaggctg
    21901 tatacgcgcc tggaacatta atatatgtgt ttgaaattat agatggttgg tgtcgcattt
    21961 attggaacaa tcataatgag tggatatggc atgagagatt gattgtgaaa gaagtgtttt
    22021 aattcttagc ttaaaatgtt aaatatttgt taattatttt ttaatgtaag tttagtttct
    22081 tttaatattt tattgatttt taatattttc tcaatataaa atgaagttgt tgatatttat
    22141 catcttaaat aagggtgtta gctataaaaa gagataaata aaaacaaata tattatattt
    22201 ggaggaagcg ccatgctcaa aagaagttta ttatttttaa ctgttttatt gttattattc
    22261 tcattttctt caattactaa tgaggtaagt gcatcaagtt cattcgacaa aggaaaatat
    22321 aaaaaaggcg atgacgcgag ttattttgaa ccaacaggcc cgtatttgat ggtaaatgtg
    22381 actggagttg atggtaaagg aaatgaattg ctatcccctc attatgtcga gtttcctatt
    22441 aaacctggga ctacacttac aaaagaaaaa attgaatact atgtcgaatg ggcattagat
    22501 gcgacagcat ataaagagtt tagagtagtt gaattagatc caagcgcaaa gatcgaagtc
    22561 acttattatg ataagaataa gaaaaaagaa gaaacgaagt ctttccctat aacagaaaaa
    22621 ggttttgttg tcccagattt atcagagcat attaaaaacc ctggattcaa cttaattaca
    22681 aaggttatta tagaaaagaa ataaaacaaa atagttgttt attatagaaa gcaatgtctt
    22741 gattgaatat gtgtagtgaa aattatcttt catcaaattc tcattcatgc acgaatggtt
    22801 cttccccacc taatcagata ttaggtgact tatggggaga aatcagttag gatgaaaaag
    22861 tggataatcc ttttttaggc aggtacttcg gtacttgcct atttttttat gttataatct
    22921 ttctagacgt attcaaggga cgtcttttta gattgtatgt tatagctagc tttcgggcta
    22981 gttttttgtt atgatgtgtt acacatgcat caactattta catctatcct tgttcaccca
    23041 agcatgtcac tgggtgtttt ttcttatgat agagagcata gttttcatac tactccctcg
    23101 tagtatatat gactttagca ttcccgtata atagtttacg gggtgctttt tatgttataa
    23161 ttaactgtat atagtaggag tgaactatat agcctgttaa gtggcctagt aacctaacac
    23221 ttatcctgca attgatatcc tttttgccct tcactcgata catatatctc aacaacatag
    23281 aaatattaca gtcgctacac cgcatcttaa atggtgtggt tatttttatt ggaagtgtgt
    23341 atcaggtatc agtaatgtta aaacaccagc taaaaatgaa aagaattcac cagtgccagc
    23401 aggttataca ctcgataaaa acaatgtacc gtataaaaaa gagactggtt attacacagt
    23461 tgccaatgtt aaaggtaata acgtgaggga tggctattca actaattcaa gaattacagg
    23521 tgtattaccc aataacgcaa ctatcaaata tgacggcgca tattgcatta atggctatag
    23581 atggattact tatattgcta atagtggaca acgtcgttat atagcgacag gagaggtaga
    23641 caaggcaggt aatagaataa gcagttttgg taagtttagt gcagtttgat aattagatat
    23701 ataaaggttt ggcaagttat gaaatgtctg ccaaaccttt atataaaaaa gaaatatcta
    23761 ccttttaatt tatgtaacta ctattagtat gcatattcat tagtttttcc aggaccatta
    23821 attacataag atgatttaga ctctcctttt ttaaagaagt atgttttata cattttacct
    23881 agtaactcaa cattttttct atcttcagca agtggtgtat tcagatatac tgtatagtaa
    23941 cctttatttt cagttaaaat aaccattttt tcaaattgag cagaattttt tgtgcctttc
    24001 tttaaataat ttctcaaacg ttcatctaat tttcctagcg ttgtaggaag accactattt
    24061 ttaaatgatt ctttataagc tttttctttc tctaacattt tcttatttga ttctatttct
    24121 tcatttgtag gaaacggttc aaaagtaaaa gctttcgctg aatgatggtg tgtactgatt
    24181 cctgccgtta aaaaacttaa tgctaaaact gttgttgcta atttcttttt cataatgatg
    24241 ttaattctcc ttaaattcta tatttaaatt tatagttagt tttgcgaaat tcctaaaatg
    24301 agtttaatct aatcgacgaa atatattaat taacttgaaa ttaataaaag attaattatt
    24361 tttaactaaa aattaaaatt caattagtgt tttgatgaat ttggtctcga taaattgaaa
    24421 taatctaaaa aacgctataa tttttctatt aatagtaatt aatatgtgct atatttatct
    24481 tagacacagc aatgtgttca aattttcatc tattcataag ctagccttcg ggctagtttt
    24541 tttgtgctat atatatttgt tttaattaaa taaaattaga taatgcaata gtagccattt
    24601 tatgttaata ttacctcggg cgttttcaag gagcgccttt cattttttat gtattgctcc
    24661 ccttcgggct agtttattaa atttattttt gcgctttcca aatcaatgta tatgtgttat
    24721 attgtttatg ggaagtaggt aagcatttcg gtgcttacct ttttttgttt ttctataaat
    24781 acaataaggt atgtcaattt gataatttat taattttcat ttaataagaa gatctatata
    24841 gttaatgaat aattaatgta ctttttttag ttagtcatta aaataaatta gtactaatta
    24901 ctaaggagaa taaaaaatga aaattagaaa atctatactt gcgggaactt tagcaatcgt
    24961 tttagcatca ccactagtaa ctaatctaga taaaaatgag gcacaagcta gcacaagctt
    25021 gccaacatcg aatgaatatc aaaacgaaaa gttagctaat gaattaaaat cgttattaga
    25081 tgaactaaat gttaatgaat tagctactgg aagtttaaac acttattata agcgaactat
    25141 aaaaatttca ggtctaaaag caatgtatgc tcttaagtca aaagacttta agaaaatgtc
    25201 agaagcaaaa tatcaacttc aaaagattta taacgaaatt gacgaagcac taaaaagtaa
    25261 atattaaaaa aaccaccctt ttacg
//
LOCUS       DAHHYJ010000020        25233 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000020 DAHHYJ010000000
VERSION     DAHHYJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25233
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.020"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..418)
                     /locus_tag="NBK22_000647"
     CDS             complement(<1..418)
                     /locus_tag="NBK22_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCD2413852.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEVVTDQKVIDWLWETQD
                     KSFFSSKEDPELCVLKVTPQSVKLMNDKSLDTPIKID"
     gene            complement(489..2594)
                     /gene="rsp"
                     /locus_tag="NBK22_000648"
     CDS             complement(489..2594)
                     /gene="rsp"
                     /locus_tag="NBK22_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCD2413853.1"
                     /translation="MTCQLKIHHTLSTIPSRNINNIMVLFSLTSKLNITINGETKDVS
                     NYIILINHGDIYNINHGENIIELMIPVFYFYQQDDDFFNGYLDRHLLQSSNYIKSLIA
                     DLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVNYIHDNI
                     DAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTKHSITTV
                     SELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTELISTID
                     LTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFEHLPKPV
                     VFIDDIHGIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILTFLQGNQ
                     DYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETYSTVEQV
                     YDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGISSTQFVY
                     NNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLYNKYGSP
                     LPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQDFIFHNE
                     LPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAVQEETGR
                     TFELKLNHDEVKYICFKPS"
     gene            complement(4082..4465)
                     /locus_tag="NBK22_000649"
     CDS             complement(4082..4465)
                     /locus_tag="NBK22_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCD2413854.1"
                     /translation="MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSIHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
     gene            4648..6090
                     /locus_tag="NBK22_000650"
     CDS             4648..6090
                     /locus_tag="NBK22_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCD2413855.1"
                     /translation="MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPV
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIIAITVTLILSKRKANKEVVE"
     gene            complement(6176..7120)
                     /locus_tag="NBK22_000651"
     CDS             complement(6176..7120)
                     /locus_tag="NBK22_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD2413856.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(7166..7789)
                     /locus_tag="NBK22_000652"
     CDS             complement(7166..7789)
                     /locus_tag="NBK22_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2413857.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNNILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGIPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIIFNGPLRIMAENRLHKSNLDSLT"
     gene            7950..9227
                     /locus_tag="NBK22_000653"
     CDS             7950..9227
                     /locus_tag="NBK22_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD2413858.1"
                     /translation="MNIFKNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQGFDFDHPNRIAIFVAFAILGFVGLILGVMVWLGMKS
                     VPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHHIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            9434..9865
                     /locus_tag="NBK22_000654"
     CDS             9434..9865
                     /locus_tag="NBK22_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCD2413859.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            9970..10086
                     /locus_tag="NBK22_000655"
     CDS             9970..10086
                     /locus_tag="NBK22_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413860.1"
                     /translation="MVNYAIYFNNININSIMALNDNNYQFERVNERNEYRES"
     gene            10097..11131
                     /locus_tag="NBK22_000656"
     CDS             10097..11131
                     /locus_tag="NBK22_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2413861.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDTHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            11167..11568
                     /locus_tag="NBK22_000657"
     CDS             11167..11568
                     /locus_tag="NBK22_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2413862.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKMREAAEKGLPNALFTVTL
                     YDKDQLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSLIMEHIMQYIKGVAVESTYV
                     SLIADYPADKLYTKFGFIPTEPDSGGMYIQY"
     gene            complement(11614..12618)
                     /locus_tag="NBK22_000658"
     CDS             complement(11614..12618)
                     /locus_tag="NBK22_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD2413863.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            12809..13324
                     /locus_tag="NBK22_000659"
     CDS             12809..13324
                     /locus_tag="NBK22_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2413864.1"
                     /translation="MAGIIKEISEQNASELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            13638..14267
                     /locus_tag="NBK22_000660"
     CDS             13638..14267
                     /locus_tag="NBK22_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413865.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDGNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSKDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNHANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(14421..16094)
                     /locus_tag="NBK22_000661"
     CDS             complement(14421..16094)
                     /locus_tag="NBK22_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD2413866.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSSINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPIATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVIVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGVDEAMLPEEALVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKTVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            16411..18009
                     /locus_tag="NBK22_000662"
     CDS             16411..18009
                     /locus_tag="NBK22_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD2413867.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKF
                     NLPGTISEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            18390..19868
                     /gene="mqo"
                     /locus_tag="NBK22_000663"
     CDS             18390..19868
                     /gene="mqo"
                     /locus_tag="NBK22_000663"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCD2413868.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFENLEQRGVGIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(19934..20389)
                     /locus_tag="NBK22_000664"
     CDS             complement(19934..20389)
                     /locus_tag="NBK22_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCD2413869.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YNHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWSITKATIMHFITTYVGFLPLAM
                     LAGWFPLTVHYLIIFTIIFIVIYVLIWIIQFFKNKNYVDTINEQLKQLK"
     gene            complement(20386..20829)
                     /locus_tag="NBK22_000665"
     CDS             complement(20386..20829)
                     /locus_tag="NBK22_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2413870.1"
                     /translation="MMKLNLFINANETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            complement(20992..22365)
                     /gene="hssS"
                     /locus_tag="NBK22_000666"
     CDS             complement(20992..22365)
                     /gene="hssS"
                     /locus_tag="NBK22_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCD2413871.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNQDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKIATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLGQVNQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFAADEKS
                     LIILADLESINFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSH"
     gene            complement(22358..23032)
                     /gene="hssR"
                     /locus_tag="NBK22_000667"
     CDS             complement(22358..23032)
                     /gene="hssR"
                     /locus_tag="NBK22_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCD2413872.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            23168..24223
                     /locus_tag="NBK22_000668"
     CDS             23168..24223
                     /locus_tag="NBK22_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2413873.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     VITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FIKLFKVDPIEAIGGAE"
     gene            24223..24888
                     /locus_tag="NBK22_000669"
     CDS             24223..24888
                     /locus_tag="NBK22_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2413874.1"
                     /translation="MALVVEDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLKNIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(24996..25233)
                     /locus_tag="NBK22_000670"
                     /pseudo
     CDS             complement(24996..25233)
                     /locus_tag="NBK22_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         8668 a         3616 c         4349 g         8600 t
ORIGIN      
        1 aatcgatttt gataggtgta tctaatgatt tgtcattcat caatttaaca gattgtggtg
       61 tcacctttaa aacacaaagt tctggatctt cttttgaact gaaaaagctt ttatcttgag
      121 tctcccataa ccaatcgatt actttttgat cagtaactac ttctatcgtg gcttccattt
      181 caacgaaact gcgatttgtc gtatcattgt agcctaacaa aacatatgct gctggattat
      241 ctttaatttc tttgacctta gcagaatgga tattcgtttt agtatataaa gtaagaccat
      301 cattataaaa gaccatatat ctactattag gtttattatt atatgcagtt gataatacac
      361 caacttttga cgttgctaac acgttttcaa ttgcttgaat tgcttgtgag ttactcatta
      421 catatcatct ccttatgata taaatagcct ttaacgtaag aaactaaaca caagaggact
      481 gttagtattt agcttggttt aaagcaaata tatttaacct cgtcgtgatt taacttgagt
      541 tcaaatgtac gaccagtttc ttcttgaact gcaagctccg ttttagggta attcgttttg
      601 tctaattcat ctaaaatttc tttttctata taaacaagtt gatcgcttat tggaagcaaa
      661 tgttgaattg aaccatgttc atgattcaat gttttaataa tcatcaaaga gtcttgaggt
      721 aattcattat ggaaaatgaa atcttgtttt acgtctgaca tatatcgatc attaatttta
      781 ttgaataata agaaatggta gttattatct ttacgactca atacataatt atttgtaatt
      841 tcaatatctt cattaacaaa aggtctaaac attttatata aatgcattaa tggtaggggg
      901 ctaccatatt tgttgtacaa ggaaatataa tttgaatggt catctaataa ttgatagcta
      961 atgccaccgc catatcgtaa taatgcgatt aagtgataca ctatgtcaga taattgaatt
     1021 ggattcttac catcattagt atatttaaat ccgcttacag acaggttatt atatacaaat
     1081 tgtgttgatg atatacctga atctaaaata aatttcttat actgttcaaa atgtgtgtca
     1141 gtttgatgat aatgttgatt tttagtgata agatgtgttg cagtttttga attttcaata
     1201 tcaataaagt aataatggaa tttaagacgc tgcatgacat cataaacttg ttccacggtt
     1261 gaataagttt ctaaaaagcc atcgactgtc acactatatt taatttcttt atttttgttt
     1321 ttaattttaa gatgacataa atgaatttct tctgctgtca tagaattaga gcaaaatacg
     1381 agcataaatt taactaattt cttgcttgtc ttatagtctt gattgccttg taaaaatgtt
     1441 agtattaatt gcttcatcgt ttcgaattgt tgtgttgatt taatagcgat tgctaaacct
     1501 atattttttt cgaaaagctt ttcaaaacat ctatttaaca ggttgtaatt cgtttggcta
     1561 atctcaatac cgtgaatatc gtcaatgaaa acgacaggtt ttggtaagtg ctcaaagtta
     1621 atatcgtaat attcattgaa aataaattga aatagttcat tgaaattact aaatcgaaca
     1681 aatactttgg cacgctgact tcgatctttc ggattaaaat catctatatg aatatctgtt
     1741 gtatttgtag ccagttgagc tgttaaatca attgttgaaa ttaactcagt gaattgtgat
     1801 acgttatctt gttggaagtg tatttgtggt ggttcagtaa tcttggaaac gagtgaacgg
     1861 aactgtttag gactaaaatg caagtaattc ttaaattgat ttgcaaaatt tgtatgactg
     1921 ttaaaaccgg ccaattcaga aacagttgta atagaatgtt ttgtagaaag taataaattg
     1981 atggcgttaa caagtttaat acttgtaaaa taatctttaa aattcatact caaatatcga
     2041 acaaaaagat tagaacaata tgattcagag atgttgcaat gcatcgctat atcttttaaa
     2101 gacaagtgcg catcaatatt gtcatgaata tagtttacac agtctatgaa caccggattg
     2161 cttaaagcaa tatttggtaa atattcatga tctattctta taaaagcttc tttaagtaaa
     2221 gtgtctataa tactttgacc tatatttttc cccattaaag aagaacttgt tggtgtactg
     2281 attaaatcgg caattaatga cttaatataa ttacttgatt gtaataaatg acgatctaaa
     2341 taaccattaa agaaatcatc atcttgttga taaaaataaa aaacgggtat cattaattcg
     2401 attatatttt cgccgtggtt aatattataa atatcaccat ggttaattaa gataatataa
     2461 ttacttacgt ctttggtttc accattaatc gtaatattga gtttacttgt aagtgaaaat
     2521 agcaccataa tattattaat attacgactg ggtattgtcg ataatgtatg atgtatttta
     2581 agttggcatg tcatatttct ctctcctgcc taatatatat tgaaatctat catttaaatg
     2641 taaaacatgg aaatttttaa aatcatggat agtataccta ttatagttga atatttttga
     2701 aaaggtaata gtgaaaaata aaagaaatta atgaaaactc ctaggaaaac gcttgttttt
     2761 atgcattgtg cacaatataa aagtaaaatc agaaagatag taagcaaaat ttggaaattt
     2821 tcgatagagt ctaattaaat tatttggtgc ataattaata tagatttcaa acctatgtat
     2881 ttcaaaaaac aatacatagc cattttgaac taacttgcta atcttttatt gatattcaac
     2941 tatgttataa aaaaagcaaa ttgaccaaaa tggaagtttg aatcaaaaaa cattaaatgt
     3001 acccatgact taaattttat cgccattatt taagtttgga catacacttt tgtcatcaaa
     3061 cgctgaaaat tagtacgatt gaaatcactt cttgttataa aaattcttag tagccctgtt
     3121 acaaaatatt cgctacaaat tattcaaatc aacaacaata accatcaact aagaaatttt
     3181 aaacaatcta ctaaaaaata aagcgtttaa aagttctgtt tagaaaacat caagataagt
     3241 ataaacatct agaaaagtga acatctaaga aaatccatct agttattgcc gtaacaatta
     3301 gaataatcat ttgaaaaagt gcattagaca acgaaattca ccatcaagat aatgaagcaa
     3361 caagaaacag cacacatcaa tgaattaaca ccttagattc atcatgagaa aatgcatggc
     3421 acaactagat ttaccatcaa gataattctt aagcagcgaa gaattaacat ctagaaatgc
     3481 ataaacatct tagattttac atcaagagat gcgataacta taaacaacta gaaacgtcac
     3541 caagatagtg caacaacaaa gaatcagcat cttgaaatcg cactaacatc taagaatcaa
     3601 catcaagtat tatattaaca tcatagataa cttcatcaag acaggcatcc acatctaaga
     3661 aactcatcta gaatagctaa cacctaacat caaagaatat tcatcaagaa cagtatctaa
     3721 cagaactttt tgactatata tgaattaatt atgttactag ctaaatcatc tattattaac
     3781 tttcacactc aatttctaca tctaatatca atttattaac aatagaatta gcagcaacaa
     3841 gatattactt aaaattactc ccccgaattt aaaatataaa caatgtaata attaagggta
     3901 taaattgatt tatgctgtaa ctaattaaat gtgtatggta tcttctaaca tcatgaaacg
     3961 catcttaata tagatatgta catttaaacc ttgagagata gattatagta aactttagta
     4021 caaagtatga attgctgaac tccaatgact actatttgtc cgttacaact aaagtttcaa
     4081 tctatctctt tctttttgtg tttaaaacac gactttgtag taaatattga acatcatatt
     4141 tattagcaag ttctgctaaa aaatctaata aagtttgggt gtcgatttta ccattaataa
     4201 aatcagcttt aagtagtttt gattgttgtc tttcttcttc attcgcacat tttttaaaat
     4261 acccccacat atgatcaaaa gcattacata cacttcctat actcggttca attaacaaag
     4321 catcatcaat catttgttgg acttgatgaa ttgataaatc cgtttttaaa gcattcctta
     4381 tcatttggta agactgttga ctgtgtaaca atacatgata tttttcctca cgccaaagct
     4441 gttcgatata gccacgttct ttcataaaat tacctccgat aattaatgtt aatatacatg
     4501 catatagtat agcaataaaa atgaatgcgc tatcaaaaat ggtgtaggtg tttatataaa
     4561 atgataatag agtgttgcaa atatttttgg aaccggttac aataaagtgg aataaatgga
     4621 accggttcta attaggaggc taaatatatg aattataagc aatccgcaga agatattttg
     4681 aacgcgatag gcggagaaga gaatttagat gcaatggcgc attgtgcaac gagactacga
     4741 ttagttttaa atgatgaaag tttagtaaat gaagaggcgc taaacaatat ggatgtagtt
     4801 aaagggacgt tttctactgg gggacaatac caaattatta ttgggtctgg tacagtcaat
     4861 aaagtattta gtgaactgga aaaattaact ggaaaagaag catcaaccac ttcggaagtc
     4921 aaagcacaat ctgctaaaaa tatgaatccg gtacagcgat ttgtaaaaat gctttcagat
     4981 atctttgttc cgattatacc agccatcgtt gctggtggtt tattaatggg gttaaataac
     5041 attttgactg cgaaagattt attcttttca ggtaaatcat tgatagatgt atatagtcaa
     5101 tttgctggat tagctgaaat gataaatgtt tttgcgaatg caccatttac attattacca
     5161 attttaattg gatttagtgc agcaaaacgc tttggtggca atccattttt aggtgctgca
     5221 ttaggtatga tactagttca tccatcgcta atgagcgcat acgatttccc aaaagcagtt
     5281 gaagcaggta aggctattcc atattgggat gtttttggtt tgcatattaa tcaagtaggt
     5341 tatcaaggac aagtgttacc tatgcttgta gcagcttata tcttagcctc aattgaaaaa
     5401 gggttacgca aagttattcc aacggtgtta gataatttgt taacaccatt gttatctatt
     5461 tttataacag catttctaac attttcattt gtaggtccaa tcactcgaca attaggttac
     5521 tggttatcag atggtttaac atggctttat gaatttggtg gtgcaattgg tggattaata
     5581 ttcggattat tgtatgctcc gattgttatt acaggtatgc atcatagctt tatagctgta
     5641 gaaacgacat taattgcaga tgccactaaa acgggtggat catttatatt cccgattgcg
     5701 acaatgtcta atgttgcaca aggtggtgca gcaattgcag cgttctttat tattaaacaa
     5761 aataagaagt taaaaggtgt ggcatctgcc gcaggtattt cagcattact tggtattaca
     5821 gaaccggcta tgtttggtgt taacttaaaa ctaagatatc catttattgg cgctatcgtt
     5881 ggatcaggta ttggttcagc atatattgct ttcttcaagg ttaaagcaat cgcattagga
     5941 actgctggat tgccaggttt tatttcaatc aatccagtac atgcaggatg gttacactac
     6001 tttgttggta tgacaatatc attcatcatt gctataacag ttactttaat tttatctaaa
     6061 agaaaagcaa ataaagaagt tgtagaataa aaaaagaggt atgacagtaa tcaaagtatc
     6121 agtcattgca aatgcaatag gatgaaactt taagctgtca taccttttaa tttagttata
     6181 atttctgttt acgccataag aaaatactaa caattacgac aagaattaat gaaataccta
     6241 ccacaataat ccaagatata taactatggt catcaattgg caacggcaca ttcataccga
     6301 agaaactaaa tacgagtgtt ggtaacgtta atagtaccgt gaaaagtgtt aatgtcttca
     6361 taatcgtatt catatcatta gataataagg aagcgtatga tgttgtgata ctttctaaga
     6421 tacgttgatg taattctgtt gtttcgatgg cttggttatt ttcaatgatt aaatcttcta
     6481 gtaattcttc atcttcttca aaacgtttaa ttgcaggtaa acggaataac tttttaataa
     6541 ttgtatcgtt accttttaag gcagctaaaa agtatactaa acttttttca acttccatta
     6601 atttgaaaag ttgtttgtta gtaatgttat tttttagttc tttctcaata cgaattctac
     6661 ttttattaag taatcgtaag ttacgattat aatggtcggc aatagtagtt aaaatttcta
     6721 atgcaaatct gctgtgaaat tttaaattaa tatcacgctt aggtaaattt tctaaaaatt
     6781 cattttcagc gtcacaaact gttacgataa taccgttgcc aataataatg cctaatggaa
     6841 tcgttacaaa agatagaacg ctacgattag ttgaattgac gataggtaaa tcaataatga
     6901 ttaatgagta accagtatct tcgtcatatt caatacggga actttcttct gagtccaaag
     6961 ggtcacgtat aaagtcttca ggtatattat attgttccat aagattttca atttcttctc
     7021 ggtctggttc tactacatta atccatgacg ctgtatgatc taatggtgtt tttataatat
     7081 gtttatctat atcttcagaa tgtctgaatg aagtaatcat gatatatagt tcctttctaa
     7141 aggatttagg aaaagcgagt attacttaag ttagtgaatc aaggtttgat ttatgcaaac
     7201 ggttttctgc cattattctt aatggaccat taaaaataat attatgcaca tgtttagcta
     7261 attcatcaac tgaaatgggc tgtttgtcca ttacccagta tttaataatt gaaattgttg
     7321 ctccggaaac gtaactgtaa aaatacatct ctggaatgcc tccgatggaa tgattgatac
     7381 taatataacg ttgcatatta tttttaatat gttcgttgat tttcaattct aattgactcg
     7441 tgcgttccaa ttgtaaaata gtatgataaa actctaaatt atcatgaata tgttggaaaa
     7501 tatttttgag tatattattt acaaaagtat tcgcaatgtc ttcaattgaa gaaccgcgta
     7561 acaaatcaaa ttgagtatat gtcgttagtt ctgaaatata ctcatcttcc atatctgcga
     7621 gtaacatata tttatcctcg taatgtaagt aaaatgtccc tctattgata tcagcgcgtg
     7681 ttgttatgtc gcgaatggta atcttttcta aatctttttc ttgtagtaat ttagtgaacg
     7741 cttgtttaat ggaggacttt gtctttctaa ttcgcctatc ttctttcaaa aataaatcaa
     7801 cctcctcgtg ttattgaaca atgtgggtgt atttgtctat taataaacgt attgatgaac
     7861 attgtgtatt gtttattccc acttacatat tataatagac aacatgcaaa atattcaaca
     7921 cattgtctaa taaggaggta attaataaaa tgaatatatt taaaaataaa ttattatgga
     7981 ttgcaccaat agcaactatg attatcttgg taatcttttc tttagctttt tatcctgcat
     8041 ataatcctaa accaaaagat ttaccaattg gtatattaaa cgaggataaa ggtacaacga
     8101 ttcaagataa aaatgttaac attggtaaaa aattagagga taaattatta gatagtgatt
     8161 ctaataaaat taaatgggtt aaggttgata gtgaaaaaga ccttgaaaaa gatttgaaag
     8221 atcaaaaaat ctttggagta gctattattg ataaagactt ttcaaaagat gctatgagta
     8281 aaacacaaaa agtagttatg gatagtaaaa aagaagaaat gcaacaaaaa gttgcttcag
     8341 gtgaaattcc accacaagtg gttcaacaaa tgaaacaaaa aatggggaat caacaagtag
     8401 aggttaagca ggctaaattt aaaacgattg taagtgaagg atcaagctta caaggttcac
     8461 aaattgcatc agctgtgtta actggtatgg gtgataatat taatgctcaa attacgaagc
     8521 aaagtttgga aacattaacg agtcaaaatg ttaaagtcaa tgccgcggac atcaatggtt
     8581 tgacgaatcc agtaaaagtg gataatgaaa aacttaataa agttaaagat caccaagcag
     8641 gtggtaatgc accattccta atgtttatgc caatttggat aggttcaatc gtaacgtcta
     8701 tcttattgtt ctttgcattt agaactagta acaatatcgt cgtgcaacat cgtatcattg
     8761 cttcaattgg acagatgata tttgcagttg ttgcagcatt tgcaggtagc tttgtttata
     8821 tttatttcat gcaaggcgtt caaggatttg attttgacca tccaaatcgt atcgcaattt
     8881 ttgtagcatt tgcgattctt ggtttcgtgg gccttatttt aggtgttatg gtatggctag
     8941 gtatgaagtc agttccaatt ttcttcattt taatgttctt tagtatgcaa cttgtaacgt
     9001 tacctaaaca aatgttgcct gaaagttatc aaaaatatgt atatgattgg aatccattca
     9061 cacactatgc aacaagtgta agagaactat tatacttgaa tcatcatatt gaattaaata
     9121 gtacaatgtg gatgtttata gggtttatga tttttggcgc tgtatcaagt ttagtttcag
     9181 ctattgttag aaaacatagc acaaaacgta ctgaagtgcc gtcataattt aataaataga
     9241 aataatagct atacaagacg tttgtagcga gcgtcatggt atagctattt tatttgtata
     9301 gcgcttgatg taaagttatt ttagctgtca tactatgtgc taaaccaaat tcagttgtgt
     9361 catatcttcg cttttttgtc actgttataa taggtataat cggatataat gaaaaggtga
     9421 gggaggattc aaaatgcgta ggttattata ttcatttctt ttttatatga tcataggttt
     9481 atttagtggc tttttctata gagagctaac aaaagcatat gattttactg gtacaacaca
     9541 attatcactt gtacacacac atacacttat tttaggtatg tttatgtttt taatattatt
     9601 accattggag aagttattta aattatcaag ttactactta tttaactggt tcttctatgt
     9661 atataacata ggtgttatcg ttactatagg tatgatggtg acaaaaggat tcttccaagt
     9721 cactggtaaa tcatactctc cagaagcatt tgcaggcttt gcaggtattg ggcacacagg
     9781 tatgcttgca ggtttattgt tgctattttt ccttttaaga caagcaattc ttaaagaacc
     9841 acgtgatgag tcttttaaaa aataatataa ctagaactaa aaaatataca catctttcaa
     9901 aggaacattg ttgtttctta ctttgaaagg tgtgttttta tattgtgatg ggatagttat
     9961 tgataattca tggttaacta tgcgatttat tttaacaata taaatattaa tagtataatg
    10021 gctttaaatg ataataatta tcaatttgag cgcgttaatg aaagaaatga atatagagag
    10081 tcataggagg cacataatga aagacgttac aatcattggt ggcggtccgt ctggtttgta
    10141 tgcaagcttt tatgctggat tgcgagatat gtctgtaaga ttgatagatg ttcaatctga
    10201 attagggggt aagatgagaa tttaccctga aaaaattatt tgggatattg gcggcattgc
    10261 gccaaaacct tgtcatgaaa ttttaaaaga tacaattaag caaggattat attttaaacc
    10321 ggaagttcat ttgaatgaac gtgtggtaga tattaggaaa aaggcagaac gtcatttcga
    10381 agttgaaaca gaagcgggag aaatatatac atcgaaagca gttatcattg caattggtgc
    10441 gggtattatt aatccaaaac aattagatgt taaaggtgta gagagatatc aattaactaa
    10501 tttacattat gttgtacaaa gttacagacg tttcaaagat aaagatgtat taatatcagg
    10561 tggaggaaat acagcattgg attgggcgca tgatattgcc aagattgcta aaagcgtgac
    10621 agttgtttat cgaaaagaag atgtaagtgg tcatgaagcg atgaaaacgt tggtgacaga
    10681 tttaaatgtg aaactatgcc caaaaacacg tattaaatac ttggtcggca atgatgatga
    10741 aacgcatata agtgaagttg ttttggaaca tgttgaaagt ggcgatacgc atacagttaa
    10801 atttgacgat gtcattatta gtcatgggtt tgatcgttgt aatacattat tgagtgaaac
    10861 atcttcaaag ctagatatgc atgatgattg tcgtgttaaa ggatttggta atacgacaac
    10921 tagtatacct ggtatctatg cgtgtggaga tattgtttat catgatgcaa aatcacatct
    10981 aattgcaagt gcatttagtg atggtgcaaa tgcagcgaac cttgctaaaa catatatcca
    11041 accagatgcg aatgcagagg gttatgtttc aagtcatcat gaagtgttta aagaagcgaa
    11101 taagactatt gtaaataaac atttatacta ggttgatata ttgtgtgatg aaaagtgggg
    11161 taaataatgg ttaaagtgac atatgatatt ccgacttgcg aggattattg cgcattaagg
    11221 attaacgcag gtatgagtcc aaagatgcgc gaagcagctg aaaaaggatt acctaatgcc
    11281 ttatttacag taaccttgta tgataaagat cagttaattg gtatgggtag agtgattggc
    11341 gatggcggaa ctgtttttca aattgttgat attgcagttt tgaaaagtta ccaaggtcaa
    11401 ggttacggca gtctaattat ggagcatatt atgcaatata ttaaaggtgt ggctgttgag
    11461 agtacatacg ttagtctgat tgcagactac ccagcggata aattatatac aaaatttgga
    11521 tttataccta ccgaaccaga ttcaggcggt atgtatatcc aatactaaat taaaaaagac
    11581 atctcgcata acgggatgtc tttttcatga atactattta tctacaccga aatcgataac
    11641 aatgcgccct ttattttcat gtttaattac tttgttaagt tgttctggaa gttcatcaaa
    11701 tgtaataact tgcttgatct catgtaaatt ttcaggcatt aaatcttttg cgagacgacg
    11761 ccaaacgcgc tgtcttaatt tcatagcagt aaataccgag tcaattccta aaatgtttac
    11821 acctcttaaa atgtgaggga atacagaatt agtataagta ttaccggcag tcataccaat
    11881 aactgcaatt gacccactat gatttaaacg ctttgtaaca taattaatac cttcgccacc
    11941 aacagggtct acacaagctt gccaagttga tgatgcgagt ggctttttat gatcatcttc
    12001 aacaggaagt cgatcgataa cttctttggc accaagttca agtaattgat cgctaacatc
    12061 ttgtttacct gtacttgcga taactttata accaagttca ttaagcatga gtactgctaa
    12121 agtaccgaca ccacctgaag caccgcgaac gagtacagga ccatcttcaa tattcattcc
    12181 aactttttca agtctttcaa ttgctaaacc ggcagtataa ccagctgtgc catatatcat
    12241 tgattcttct aatgttaaag tatcaggaag cttgataatc cattctgatt ttacacgcgc
    12301 atattcacta aaaccgccat aatggctaac gcctaggtca taactcgtta caattacttg
    12361 ttctcctttt tcaaagcctg gtgcttcgga ttcaacaatt gttccagcta aatctattcc
    12421 tggaatcata ggatacgatt ttacgactgc attatgatct tgagtcgcta aagcatcttt
    12481 ataatttata ccagagtaat gtactttaat cagcacatct cctttaggta aatcagtagg
    12541 cgataattgt ttgaaagttg gtgtcacttt accgctctca tctttatcaa ttacaaacgc
    12601 tttaaatgtt tctatcacaa cgatacatcc tctctttttc tcttttgaaa atacggccgt
    12661 tttactggaa taaatgtatc atttattgat gttacacaca atcaatacaa tttgtaattg
    12721 atttgtgtta atgtattttt aaatagtttt ggctgaaaaa taacataagt aatattttga
    12781 ttcgatcact gtttattgga ggatttatat ggctgggata atcaaagaaa tttcagaaca
    12841 aaatgctagt gaattagttg aattagcaac tagaacattt tatgacacgt ttggttctta
    12901 ctatgatgac aaagattttg atcaattttt taaagacaat tatactgtag aaaaatttac
    12961 acaagagatt aaccatgtag attcatttca ttatttttat caagaagatg gtgcgaatgt
    13021 tggttatata aagatgaata ttaatagtgc tcaaactgaa gaaatggggg agacctattt
    13081 agaagtgcag cgcatatatt ttttgaaaga ctttcaaggt ggcggaagag gttcacaatt
    13141 gatagaattg gccgaaaaaa ttgctcaaga acataataaa cataaaattt ggctaggagt
    13201 ttgggagcat aatcctcgtg ctcaagcatt ttataaacgt catggattta aagtggttgg
    13261 tgaacatcat tttcaaacag gtgatgtaac ggatacagat ttaattatgg aaaaagagtt
    13321 ataatgattg tttttgcagc agataaaatg aaatcatagt aataaatatt gtgtatgcct
    13381 tgaaatgcta aatgcgtaat gaagtgttaa agtaaaataa ttaaatttat agaaaacata
    13441 aaattcacaa aaattaaata ataatgaaca aaaattaggg tataataaaa agaaccttag
    13501 attgtagtta tggatattgt gataattcag atcatattaa tgttgacgtc tttggtaaca
    13561 agccatgaaa agatacacat ggtagatatg tatttcaaga ttattcaatg aatatcgaat
    13621 tataggagga gatatgtatg aaaagattag ttacagggtt actagcatta tcattatttt
    13681 tagctgcatg tggtcaagat agtgaccaac aaaaagacgg taataaagaa aaagatgata
    13741 aagcgaaaac tgaacaacaa gataaaaaaa caaatgattc atctaaagat aagaaagata
    13801 ataaagatga tagtaaagac gtaaacaaag ataataaaga taatagtgca aacgataacc
    13861 agcaacaatc taattcaaat gcaacaaaca atgaccaaaa ccaaacaaat aataaccaat
    13921 caagtaataa tcatgcgaat aataatcaaa aatcaagtta cgttgcacca tattatggac
    13981 aaaatgccgc accggttgca cgtcaaattt atccgtttaa tggaaataaa actcaagctt
    14041 tacagcaatt gccaaatttc caaacagctt taaatgcggc taataatgaa gcaaataaat
    14101 ttggtagtaa taataaagtg tataatgatt attctattga agaacataat ggcaactata
    14161 agtatgtgtt tagttttaaa gacccaaatg caaatggaaa atattcaatt gtaacggttg
    14221 attatactgg acaagcaatg gttactgatc caaactacca acaataatgt taatataact
    14281 atgatgcaag ttaaaaaaat aaaacggtaa actctataat atgaattagg gtttaccgtt
    14341 ttttgcgtat tttaaagtat aaaagtttac gttttatagc acttttaaac ctcattcaca
    14401 gccactgaga aatttaaaca ctattgatta gatgtcatct tagacaatat aagttgtgaa
    14461 atagattcta atggttgttc tttatgttgt atttgtggat atttaatcac tggtattgcc
    14521 tgggaaatta aattattaat taataattgt cggtctgtat accaaatttt tgatagatga
    14581 cgccaaatat cttcgttaac accttgagac tcaatgtatt gactgtgatt tggagtgtat
    14641 aatgctactg ttttgtctat agccatcgca tcaaatatta tatttgagta atcagtgata
    14701 acgacatcag acattagaat taagtcttga gcactaagat actttggtgc aacaagcgcc
    14761 tcttctggta acatcgcttc atcaacaccc tgaaccacaa catgataggc tttgaataaa
    14821 gcatctgata gtggtaattg ttgcgcactc actaatccaa taggtgcgta taataaaact
    14881 ggtttttgat catttatttt aaacgatttc ttatactgtt gttgagtagt actttcgtta
    14941 cgcttttgta gtaaatattg gtgtttagga ttaccataag ccaatacatt tgcttggtga
    15001 ctaggaaaag ccgtttgata aaagtggcta atatcatcag cagaatgtat aacataatcg
    15061 aattgcaacc agcgattata ccttttagca cgataaaaag gtgtttctat atttttaata
    15121 ggctctttac tatctaaaaa taattgcatt attggtgtgc cttgatgtaa ttgaattaaa
    15181 gtaccgtttg gttgtaacgt ttcaggtata tcattttcaa ctataactac gcgtgcgctg
    15241 ttaatcaatt cgtcagcttt ttcagactta ggtgatatga aatgtggtcc gcgacgatcg
    15301 tcagtaacaa aataacaggc agtcattgga ttatgtttaa cgaagtaagt aaataaatat
    15361 tttgaattac ctatatattg atgatgatca ccgataaaaa cgatgtgatc ttttaaaacg
    15421 tttggttgct tcgtatacag gccgaagttt tgtaattgtt tgttatcagc acgttgcttt
    15481 gctattttat ataaccattt aggtagcata aacttggtag aatacaacca attatctggc
    15541 gaattaagtt gttctgaatc ctcgataaaa aggttgcgaa aaggtatagg atgatgatca
    15601 atataagcca ttttattcaa ataaaactga gacatcatat caaaatcttc aatagtttta
    15661 atagtttccc atattgaagc acttgttgct ataggggctt taaaatcaaa aacgacatga
    15721 ccatcgattg ataaatcacc tttcagaagt gtcaataaag ctaaatcgaa aatcgtttct
    15781 acagagtaat aatgtattaa atcaagagtc tgttgaatcg catatatcag gttaggataa
    15841 taattattgt tttgagccca atcggcaatg ttgatcgatg attgaccgaa atagtgacaa
    15901 tcatttttgt attgtccatc cacagtataa tcaaccacga tagacttagg atataattga
    15961 accgcatcta acatcgattg ataaatcatt atctcatttg catatggaac gacatgtgta
    16021 taaccttcag actgagcttt cattaattga gattcaaact tttgcatatt agaaacattt
    16081 atctgtttaa tcattgctat aacttccatt tctcatcgta gtatcttcct tattttagca
    16141 cggtcttatg taaaattcat gaatagtata agttacttca ttacattaag tacaattcat
    16201 tttgatgaac agatttaaaa tgaaaaataa aacaattaaa taggaaagtg atattgttaa
    16261 tagtctgaaa aaatggtata ttttaattgt gaaaaaaatc acattaatct cactgttctg
    16321 taaaatgtta tcgataacaa aatgctatag ccagagcatg attttaatga agcaattaca
    16381 ctactacttt atggaaagaa gagtcagcct atgttagtaa atacgtttaa cccatttgat
    16441 aatttattat tatcgtcttt aattgcagca ataccgattg tactgttttt actatgttta
    16501 actgttttta aaatgaaagg tatttatgca gctattacaa cacttgttgt aacattacta
    16561 attgcaatac catttttcaa attgccagtt ggtatcgctt ctggtgcagt agtcgaaggt
    16621 ttcttccaag gtatcattcc gattggctat atcgttatga tggcagtatt gttatacaaa
    16681 attactgttg aatctggaca atttttaaca attcaagata gtattacaaa tatttcacaa
    16741 gaccaacgta ttcaagtttt acttattgga tttgcattca acgcattttt agaaggtgca
    16801 gcaggatttg gtgtaccaat tgcaatttgt gcacttttat taacacaatt aggatttaat
    16861 ccattaaaag ctgcgatgtt atgtttagtc gcaaatgcag cgtctggtgc ttttggtgcg
    16921 attggtatcc ctgtaggtgt tgtagaaacg ttgaaattac ctggagatgt ttcagtatta
    16981 ggtgtttctc aatcagcaac tttaacattg gcaatcataa atttcattat tccattctta
    17041 cttatcttta ttattgatgg ttttagaggt gttaaagaaa cattaccagc aattttagta
    17101 gtttcaatca catatacact tactcaagga ttattaactg tattcagtgg acctgaattg
    17161 gcagatatta ttccaccgtt attaacaatg ttagcattag cagtattttc taaaaaattc
    17221 caaccaaaac acatttatcg tgttaataaa gatgaagaaa ttgaacctgc aaaagcacat
    17281 tctgcaaaag cagtattaca tgcatggagt ccatttattg tattaacagt cattgtaatg
    17341 atttggagtg cgccattctt taaaaactta ttcttaccaa atggtgcttt atcatcatta
    17401 gtatttaaat tcaacttacc tggaacaatc agcgaagtta cgcataaacc attagtattg
    17461 actttaaata ttattggaca aacaggtaca gctattttat taactattat tattacaatt
    17521 ttaatgtcta aaaaggttaa ctttaaagat gctggtagat tattcggcgt tacatttaaa
    17581 gagttgtggt taccagttct tacaatttgt ttcatcttag caatttctaa aatcacaact
    17641 tatggtggtt taagtgcagc aatgggtcaa ggtattgcta aagcaggtaa tgtcttccca
    17701 gttctatcac caatcttagg ttggataggt gtgtttatga caggatcagt tgtaaataac
    17761 aactcattat ttgcaccaat tcaagcttct gttgcacaac aaattggaac aagtggttca
    17821 cttcttgtat ctgctaacac agttggtggt gtagcggcaa aattgatttc accacaatca
    17881 attgcaattg caactgcagc agtaaaacaa gttggtaagg aatcagaatt attaaaaatg
    17941 acattgaaat acagtgtatg tttactaata ttcatctgta tttggacttt catcttgtca
    18001 ttattataaa ataacgtatt tcaaaatata aatatacaga gggtgagatg ttttctaaca
    18061 tctcatcttt ttttaatgga ttattaatga aagaagtttg acattataat aatggtagcg
    18121 ctttatgtta aaatgaatag tgagtaatca gcaatcaaat taaattggtt gatagctgtt
    18181 aaggtttgtg gttttgtctt tgtgctatcg cacataaagt atataattaa agtagtttcg
    18241 ttattataaa atattaatat acatagtaga tagtaataga gcatcaccat gggaacctat
    18301 tgagacactt attgatttaa agtggtatta atatgtcgta tttctcgaac gttccattat
    18361 tcattttaaa aagggggact gtatttgtta tgacaacaca acatagcaaa acagatgtca
    18421 tcttaattgg tggcggtatt atgagtgcaa cattaggaac attacttaaa gaattatcac
    18481 ctgagaaaaa tattaaagtg tttgaaaaat tagcacaacc tggcgaagag agttcaaatg
    18541 tatggaataa tgccggtaca gggcattcag cactttgcga gttgaactat acaaaagaag
    18601 gtaaggatgg cacagttgat tgtagtaaag caattaagat aaatgagcag taccaaattt
    18661 caaaacagtt ttgggcatat ttagttaaaa caggacaatt agataaccca gatcgcttta
    18721 ttcaagcggt gccacacatg agttttgtca ttggcgaaga taatgtagct tttataaaaa
    18781 gtcgtgttgc aacgttaaag aaaagtgttt tattcgaaaa aatgaaatta tcgcaagatg
    18841 aagaagaaat gaaatcttgg gtaccgttaa tgattgaagg tcgtaagtct gatgaaccaa
    18901 ttgctttaac ttatgatgaa actggtacag atgttaactt tggtgcgtta actgcaaagt
    18961 tatttgaaaa tttagagcaa cgtggtgtgg gaattcaata taagcagaat gtattagaca
    19021 tcaagaaaca gaaatctggg gcatggctag ttaaagttaa agatttagaa actaatgaaa
    19081 cgacaacata tgaatctgat tttgtattta ttggtgctgg cggtgcgagt ttaccattac
    19141 tccaaaagac tgggattaaa caatcaaaac atattggtgg tttcccggta agtggattat
    19201 tcctgcgctg tacaaatcaa gaagtgattg atcgtcatca tgctaaagtg tacggaaaag
    19261 cagcagtggg tgcgccacca atgtcagtgc cgcacttaga tacacgtttt gtagacggca
    19321 agcgttcatt gttatttggt ccatttgcag gtttctcacc taaattttta aaaacaggtt
    19381 cacatatgga tttaattaaa tcggttaaac caaataatat cgtgacgatg ttatctgcag
    19441 gtatcaaaga aatgagtctt acgaagtatt tagtgtcaca attgatgtta tctaatgatg
    19501 agcgtatgga tgatttaaga gtctttttcc caaatgctaa aaatgaagat tgggaagtga
    19561 ttacagcagg gcaacgtgtc caagtaatca aggatactga ggattctaaa ggtaacttac
    19621 aatttggtac cgaagtcatt acgtcagatg atggcacatt agctgcatta cttggtgcat
    19681 cacctggtgc gtcaacagct gtagatatta tgtttgatgt tttacagaga tgctatcgtg
    19741 atgaattcaa aggatgggaa ccaaagatta aagaaatggt gccgtcattt ggttatcgct
    19801 taacagatca tgaggattta tatcataaaa ttaatgaaga agtaactaag tatttacaag
    19861 ttaaataata aacgaaacgg taatgtcttt tttaatgtga tagacattac cgttttttag
    19921 ttgttaataa aaatcatttt aattgtttca gttgctcgtt aatagtgtct acgtagttct
    19981 tgtttttaaa gaattgaatt atccaaatta atacataaat cacaatgaag ataattgtga
    20041 atatgattag ataatgcact gttagtggaa accaaccggc aagcattgct aaaggcaaga
    20101 atccgacata cgttgttatg aaatgcatta tagttgcttt agtaatgctc caatctgtgt
    20161 atttaaagat aaaatctcca aggaaaaaga cgacgcctat gagtaaccat aaaatgatag
    20221 aaatcaacat tacggtagtt tctgtgaaat gattataata caatatgcca atagttgatt
    20281 gtgggttcag tggataatat ttgccgtctg caaataacat actaaagaac agtgaaaggg
    20341 acaaaccaat gattaagcta ataaataatg agtttttcaa atttttcata ttgataagcg
    20401 ctcctttata gattttaaat aacgtctaga agaataggtg tagtgtgcat ctttaagata
    20461 catacgtata agtccatttg gctctaataa taatttttca atgtaatact tgttgacgat
    20521 ttctgattta gaaatgcgaa tgaaatgttg tggtaactgt ttttctagtt cataaagtcg
    20581 taattttagt ttgaattttt gattgcttgt aatggctgtt acatttttat taataacttg
    20641 gaatgttata acatctgata cattaatgat atggatttct ttgtctatgt atccgactaa
    20701 tgtatgtgat ttgtctaaat cattgactgc attaataata ctttgaacgt gatcattcat
    20761 tttaggtgca tgtatatcaa tataagattc cgtctcattt gcattgataa ataaattgag
    20821 tttcatcata ggttaatgcc tccttcaaaa ttattaaacc ataaatgacc atcgatatat
    20881 ttaaattttg ttgaatggta gaaattaaat gttaagtggc tagaaagcgc taatcaatat
    20941 aaaagatacc tcctgaaata aaaacagaaa tgttttttca ggaggtagag attaatgtga
    21001 attatttggc agtgtaatag taaaggtggt tacatactcg ttactttgtg tgaattggat
    21061 tgtaccatga tgcaattcaa tgatggattt tgtaattgca agacctaaac cattgctatt
    21121 atcatgtttg ctcactttat aaaaacgttc aaataaacgt gcttcagctt gtggactaat
    21181 tggtgaacca tcattactta ttgtaaaaat gatgttgttg tgactatgtt gcaaagcgat
    21241 gtcaatggca ccaccaacat ctgtatactt aatagcattt attaataaat tactcaatgc
    21301 ttgatgtaac aaacgttgat ttcctaggaa attgattgat tctaggtcag ctaaaataat
    21361 taatgacttt tcatcagcag caaattgttc atgtcgaatg atatctttaa tgagttgatc
    21421 gacttgaatc ttatcatcga ataataaatg ttggtgatta tctaattcgg ataagagcaa
    21481 caattcagtt gttaatccgc tcagctgcgt tgtaatggta taaatatcat ttatatattg
    21541 ttgtcttaat gttttatcag aagtttgttg taactcgctt aataaatgat gaatatgtgt
    21601 taatggtgtt ttgatttcat gagatacatt ttgtacaaaa tgctgcctca tttgattaac
    21661 ttgtcccaat gactctctca tcttattaaa gtgatactgt aatgttccaa tttcatcttt
    21721 gcgtgtttgt ttgataggtg tttcaaaatc accatcaatt aagcgttcgg tcgctatctt
    21781 taactttttt actggacgaa tgattgaata ggttgatgcg ataactaatg aaatcgagat
    21841 aaataataac aacattaaca agacggctaa gaatgttcta aactcgctaa atgtttctcc
    21901 aatatctgga cgcataaata ctgctattga accgtctttt gtcttaaagt taataccaac
    21961 agtattatct gttacattgt cgaaaaatcc agttacgaat aatgcgaatg gtttatcttt
    22021 aataccatgg taatcttgat tatttaatac gttgttaata gcattttgtg ataaagtatc
    22081 ttctctaaat ggctccccat aaaatgtctt atgacctttt tgatcaacgg tcattatttg
    22141 gtaattcatt tgccctaaat gtttgaaata ttgttgaatg tgcgttggtt tagctgattg
    22201 ttcatattgt cttgcttctt taagtgtctt catgattttc gcgtcattag atgcttttaa
    22261 attataatga tagtaaacat ttgttaatac aaaacttatt aatgcactaa ataaaataac
    22321 cgtaatggaa taaatcgcaa ttctagcata gagtgtttta aacatgattc tccaccttat
    22381 agccttgtcc tcttactgtt tcaattgtaa gtgtggcatt taattttttt aatctttggc
    22441 gtagtcgctt aatatgaacg tcaactgttc gttcatctcc ttcataatca tagccccaaa
    22501 ttttttctat tatctgttca cgtgtaaaga tttgcttagg acgtgctgca agcataaata
    22561 ataattgaaa ttccttgttc ggtaacgtca tcgttttatt agatacttgg agttccaaat
    22621 aggattggtt tagcgttaag ttgccaatag tcatttctga atttgaattg atattatatc
    22681 gacgtaatac cgcacgaatt ctaaaaataa gttccttaac ctcaaagggt ttggttacat
    22741 aatcgtcagt accgcttata aacgcacgct ctttgtcact aagtgcatcc cgcgctgtta
    22801 acataataac tggtatatca taatcatttt ttaatgtatt acataattga aagccgtcca
    22861 taccatccat cataatatct accactgcaa tatcgacacg ctgtttttct aaaagtttta
    22921 atgcagcttc accacttggt tgtgtgtatg catcaatgtg ctctgtttgt aaatggctag
    22981 ctatataatt taggattcga ggatcgtcat cgacaacaag acattgcacc atagctataa
    23041 actcccttat ctttttcatt tattatacat gtaaaatatt tttgcgtaaa aaaacaattg
    23101 ttcatattga gttcatattt caaccttata ctgacgctaa agaagaaata gggagaagtg
    23161 aatcgatatg aaattagcga taaaagagat tatgttttac aaatttcgtt atattttaat
    23221 cacattaatc attcttttat taagtattat ggtgttattt attagtggtt tagctcaagg
    23281 gcttggtagg gagaatattt cgttatttga acactttgat aatgatgaat atgttgttca
    23341 aaaaatgaaa gagccgcaaa ttgagaaatc gcaacttagt gatacgcaac agaatcaaat
    23401 taaaaaggta attcatcaag aaccatataa aatgaatatt caaacattga aattatctaa
    23461 taaagaacaa gatgtgatta caatgaatga cgttaaacaa caacgcatac aacttaaaaa
    23521 aggtgattat cctaaaaatg ctcatgaggt cgctattaat gataagttag ctgcagacaa
    23581 cattagagtc ggggatagat tacattttaa aaataattca actagttata gagtttctgg
    23641 tattttaaac gacacaatgt atgcgcatag ttccattgtg ctattgaacg ataacggatt
    23701 taatgcattg aataaggtta atacggcatt ttatccagtg aaaaatttaa cacaacaaca
    23761 acgtgatgag cttaataaaa taaatgacgt tcaagttgtg agtgaaaaag atttaacagg
    23821 taatattgcg agttatcaag cagagcaagc accgttaaat atgatgattg ttagtttgtt
    23881 tgctattaca gcaatcgttc taagtgcatt tttctatgtt atgacgattc aaaaaatatc
    23941 acaaattggc attttgaaag caattggtat taagacaaga catttattga gtgcgttagt
    24001 tttacaaatt ttaacactaa caataattgg ggtaggtatt gctgtgatca tcatagtagg
    24061 actatcattt atgatgccgg taacgatgcc tttttactta acaacgcaaa atattttatt
    24121 aatggtgggg atatttatat tagtagcgat tttaggtgcc tcactatcat ttatcaaatt
    24181 atttaaagtg gatcctatcg aagcaattgg aggtgcagaa taatggcatt agtcgttgaa
    24241 gatatcgtca aaaatttcgg agaaggtttg tctgaaacaa aagttttaaa aggtattaat
    24301 tttgaagtgg aacaagggga atttgtcatt ttaaatggtg cctctggttc tgggaaaaca
    24361 acattgctaa cgatattagg cggattgtta agtcaaacga gtggtacagt gctttacaat
    24421 gatgcgccat tgtttgataa acagcatcgt cctagtgatt tacgattgga agatattggt
    24481 tttatttttc aatcttcaca tttagttcct tatttaaaag tgatagagca attgacactc
    24541 gtaggtcaag aagcgggaat gaccaaacaa caaagttcaa caagagcaat acaacttttg
    24601 aaaaatattg gtttagaaga tcgcttgaat gtatatccgc atcagttatc tggcggtgaa
    24661 aagcaacgtg ttgcgattat gagagcattt atgaataatc cgaaaatcat tttagcagat
    24721 gagcccacag caagtttaga tgccgataga gcaacaaaag ttgttgagat gatacgtcaa
    24781 caaattaaag aacaacaaat gattggtatt atgattacac acgatcgaag attatttgaa
    24841 tatgcagatc gagtgattga attagaagat ggcaaaataa ctgattagtg gcttgtaaag
    24901 acgctaaatg ttaatgattt aagacatagt agtataaaag ttagataaca gaatacgatt
    24961 tgggtttaca aaaaacaggc tgggacatta agttcttagg caatgtaaaa aagctgattt
    25021 ctattaatta tttgatagaa atcagctttt ttgatatgta ttttataatg tacagctcgt
    25081 tgcattcata tagcttgaag tcacgtttaa aaccatatct atcattatgg tatgcatatc
    25141 ttttaaaacc tattcttttg ttattaggac atataaattc atcattaagt tcgtcatatt
    25201 tccaattttg agtgttaaaa atgtcacttt taa
//
LOCUS       DAHHYJ010000021        25088 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000021 DAHHYJ010000000
VERSION     DAHHYJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25088
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.021"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..171
                     /locus_tag="NBK22_000671"
     CDS             <1..171
                     /locus_tag="NBK22_000671"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413875.1"
                     /translation="REFRNEILQAMQVGDGPQQREVGFPISTDNASWQGPNIEAGGKS
                     AYNNVQVGGAPT"
     gene            complement(284..2890)
                     /gene="yfhO"
                     /locus_tag="NBK22_000672"
     CDS             complement(284..2890)
                     /gene="yfhO"
                     /locus_tag="NBK22_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCD2413876.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKFICVISATVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLALIVGIILLMVLAVILKFSLWRYKKLTVAILVLIVMIQQIVILD
                     NNKNMAIKPYQQSISTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDDNLPYGFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNTKDVNTHFKANKNLLSDSTIKLNSAAWQSPTKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHDVKVNEYTQERNKLTYKYRRFVTPVTIRIKASDRI
                     RLSLPKGKYRVNLKGIYGEDYTTLKDASNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWARQK"
     gene            3291..4175
                     /gene="ylqF"
                     /locus_tag="NBK22_000673"
     CDS             3291..4175
                     /gene="ylqF"
                     /locus_tag="NBK22_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCD2413877.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKSHYNIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            4159..4926
                     /locus_tag="NBK22_000674"
     CDS             4159..4926
                     /locus_tag="NBK22_000674"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCD2413878.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVRNAIARRRKA
                     LEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPFTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            5035..6201
                     /gene="sucC"
                     /locus_tag="NBK22_000675"
     CDS             5035..6201
                     /gene="sucC"
                     /locus_tag="NBK22_000675"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCD2413879.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEALEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKESVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            6223..7131
                     /gene="sucD"
                     /locus_tag="NBK22_000676"
     CDS             6223..7131
                     /gene="sucD"
                     /locus_tag="NBK22_000676"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCD2413880.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYEALLTVNK"
     gene            7455..8327
                     /gene="dprA"
                     /locus_tag="NBK22_000677"
     CDS             7455..8327
                     /gene="dprA"
                     /locus_tag="NBK22_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCD2413881.1"
                     /translation="MLNHILLKLIWSRFTTAQIHLLLKTFPSCCEVDEYERMHILENF
                     CKLLADKKIDAKVNRYKEISVDEIETTIQKTQLNYITCFDHNYPYLLKETYNYPIILF
                     YKGNINLLSYPHTLAVVGSRLSGDYTLKALNHLFVSFQQMSFCVVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTSALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            8501..10576
                     /gene="topA"
                     /locus_tag="NBK22_000678"
     CDS             8501..10576
                     /gene="topA"
                     /locus_tag="NBK22_000678"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCD2413882.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            10732..12039
                     /gene="trmFO"
                     /locus_tag="NBK22_000679"
     CDS             10732..12039
                     /gene="trmFO"
                     /locus_tag="NBK22_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCD2413883.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
     gene            12456..13352
                     /gene="xerC"
                     /locus_tag="NBK22_000680"
     CDS             12456..13352
                     /gene="xerC"
                     /locus_tag="NBK22_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCD2413884.1"
                     /translation="MNHIQDAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLEL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKNLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            13349..13894
                     /gene="hslV"
                     /locus_tag="NBK22_000681"
     CDS             13349..13894
                     /gene="hslV"
                     /locus_tag="NBK22_000681"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCD2413885.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEGKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            13960..15363
                     /gene="hslU"
                     /locus_tag="NBK22_000682"
     CDS             13960..15363
                     /gene="hslU"
                     /locus_tag="NBK22_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCD2413886.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            15388..16161
                     /gene="codY"
                     /locus_tag="NBK22_000683"
     CDS             15388..16161
                     /gene="codY"
                     /locus_tag="NBK22_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCD2413887.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            16212..16304
                     /locus_tag="NBK22_000684"
     CDS             16212..16304
                     /locus_tag="NBK22_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413888.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDISH"
     gene            16503..17270
                     /gene="rpsB"
                     /locus_tag="NBK22_000685"
     CDS             16503..17270
                     /gene="rpsB"
                     /locus_tag="NBK22_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCD2413889.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            17304..17417
                     /locus_tag="NBK22_000686"
     CDS             17304..17417
                     /locus_tag="NBK22_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413890.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            17452..18333
                     /locus_tag="NBK22_000687"
     CDS             17452..18333
                     /locus_tag="NBK22_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCD2413891.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            18470..19192
                     /locus_tag="NBK22_000688"
     CDS             18470..19192
                     /locus_tag="NBK22_000688"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCD2413892.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPIIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEEGNIKRAVMGEKIGTLITK"
     gene            19211..19765
                     /gene="frr"
                     /locus_tag="NBK22_000689"
     CDS             19211..19765
                     /gene="frr"
                     /locus_tag="NBK22_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCD2413893.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGAEDVQ
                     KATDNSIKEIDQMIADKEKDIMSV"
     gene            20138..20908
                     /locus_tag="NBK22_000690"
     CDS             20138..20908
                     /locus_tag="NBK22_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCD2413894.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRIASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            20915..21697
                     /locus_tag="NBK22_000691"
     CDS             20915..21697
                     /locus_tag="NBK22_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCD2413895.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQS"
     gene            21909..23195
                     /gene="rseP"
                     /locus_tag="NBK22_000692"
     CDS             21909..23195
                     /gene="rseP"
                     /locus_tag="NBK22_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCD2413896.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASERTLFKPIVYGFESFLKGSTLIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            23215..24918
                     /locus_tag="NBK22_000693"
     CDS             23215..24918
                     /locus_tag="NBK22_000693"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCD2413897.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHSTVQPLEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNL
                     K"
BASE COUNT         9198 a         3498 c         4830 g         7562 t
ORIGIN      
        1 agagaatttc gaaatgaaat tctacaggca atgcaagttg gggatgggcc ccaacaaaga
       61 gaagttggat tcccaatttc tacagacaat gcaagttggc agggccccaa catagaagct
      121 ggcggaaagt cagcttacaa taatgtgcaa gttggcgggg ccccaacata gaagctggcg
      181 gaaagtcagc ttacaataat gtgcaagttg gggtgaacaa cgaaataaat tttgcgaaaa
      241 tatcatttct gtcccactcc cattaaatat atgcgtagca acattatttt tgtcttgccc
      301 atctcgtgaa aataatacta catataatgc caaatatagt aattgttata agtaaataat
      361 agtatggtgg ggtatagctc aattgaatct ttgttatatt tttaggtgct ttaatgccgg
      421 tcataacacc atttacttgt tcaactttaa gactttgatc acctgatgtc gctttcatac
      481 cttgattata tgctgttggc aaaacaatat acccagatga atttttattt ttagtaatag
      541 tataaccttg ctttgtctta ctaactttga cagcctctaa tgaatttgaa gcgtctttaa
      601 gcgtggtata atcttcgccg tatatccctt ttaaatttac tcgatactta cctttaggca
      661 atgataatct aattctatct gaagctttaa tgcgtatcgt tacgggtgtt acaaagcgtc
      721 gatatttata agtgagttta tttctttctt gtgtatattc attcacttta acatcatgag
      781 ctttatccgg cgaaagtaat tctaaatcca tttcaaaata caaatcttta aattgattag
      841 aaactgattt tggcaactgt acagttagac caccattatt ttgtttaact tgtaataaat
      901 gttttgtagg agattgccag gctgcactat ttaatttaat tgttgaatct gatagtaaat
      961 ttttattggc tttaaaatgt gtattaacat ctttagtatt gttagaaaca atcccttgca
     1021 acattgcttg ttctttatct aatggagatt ttaattcttt attggaaaag accttatttg
     1081 taatatgtgc acttggataa tggatggtat ttttagaatg aatccaacga actttattgt
     1141 ctttgtgttc agagttaatt ttaaatccat atggtaagtt gtcatcatga tttactctaa
     1201 ttcgatcatt cacattccaa agcgatagta aattttgacg attgccaagt aatctataag
     1261 tgctgttttt atcgattggc atattaattt ggagtgtctt gtcataatat tttaaaatgt
     1321 ctccattaaa aatactagaa tataatgaaa tgccattata atgatatata aatggtgaat
     1381 ttaatgcata gtctgacata taatcaatgc gattaaatgg gcctgttgca ttttgattta
     1441 tcttttttat tagctggttt acatagttac tatgataatc atgttgtttc aacgttgata
     1501 ttgattgttg ataaggtttg attgccatat ttttgttgtt atctaaaatg acgatttgtt
     1561 gaatcataac gattaatact aatattgcaa cggttaattt tttataacgc cataaactaa
     1621 attttaaaat aacggcaagc accattagca ggataatacc tactataagt gcaagtgggt
     1681 gtgtcggtga tagtaataca taaagtaatg cgatgatact tactggtatt gttctgatta
     1741 aataatattt catatttaat gttgataaat gttgaataaa caatccgcaa agagcgcttg
     1801 atgatagtgc taagatatac acccaacgcc tttctggaaa tgaaaaacca ttaaaagcac
     1861 tgtcgaaata ctgtgataat gaaccaataa ataatatcca tgttactatt gcgaaaagtc
     1921 tataaaagta aaaacgatac agtttgaatg acaataatgc aacgatagta agaattgaaa
     1981 tcgtaatata aaatccatcg ctaaagaaaa aataatgata atcaagtggt gtcaaaaacg
     2041 gtatatcaac attgggattt tgctttctgt cattttccaa aaacgcagaa atgccagtga
     2101 ataaaccaaa tacacttgat aacacactca aaactgtagc agatatgacg caaataaatt
     2161 tttgtgttct agagacaatg tcatatttgt aagtgaaaat gagtcgatat aaatagtagc
     2221 aacctataat aatagcttga taataactga aataaaaatt gctaaatagt gttaaggcta
     2281 tcgcaacaat gaaaataccg attttgcgtt gttgaaaata tctttccaaa ccaagaatcg
     2341 ataatggcaa taaatataat aaatttccat aaaatgacca agtaaaatta aagtatataa
     2401 cgacagttga catgccgtat aaaatcgtag cgatcatatt tgttgagcgt ttaaagtgta
     2461 atattttaaa taagtagaag gtcacgacaa atgttatgat agctcgtatc atggccataa
     2521 taagttggtt tgtcggccaa aaatgtattg ttgtcggatt aaatatacca accgtttctc
     2581 ctattttaat gaatagaaaa tttagccaca ttaaaggtga cagcgaataa taatatgata
     2641 gtcctttcat ataatcgcca cctaatccaa acgatgcatc atataaacta gaaaaactac
     2701 ttaaatgttc atacaaatac atttgaaatg gcatcatttg acggaatcca tctccagccc
     2761 cactaaaaac agtaccattc acaatataat catagatatg agtagaaaat aaaataagcg
     2821 ttaatattac actaatgaaa gttataacaa agaattgttt gacgtttgaa tttagccact
     2881 tttttaacac aacattatcc tcaactttca aatttaaaat taagtttaac tgaaactaaa
     2941 gttaatgagg ttcttgatag gtaaagacga agatgactgt ggaacagata ccttatcata
     3001 gttacttaaa ctttggatca ttttcagttt atcattaaac aaatatattg aataataaaa
     3061 atgtcatact gataaagatg aatgtcactt aataagtaac ttagatttaa caaatgatga
     3121 tttttaattg tagaaaactt gaaataatca cttataccta aatctaaagc attgttaaga
     3181 agtgtgacaa tgttaaaata aatatagttg aattaatgaa tttgttctat aattaacagg
     3241 ttatagattt taataatgag aaaagaattg acgaaagtaa ggtgaattga atggttattc
     3301 aatggtatcc aggacatatg gcgaaagcca aaagagaagt aagtgaacaa ttaaaaaaag
     3361 tagatgtagt gtttgaacta gtagatgcaa gaattccata tagttcaaga aaccctatga
     3421 tagatgaagt tattaaccaa aaaccacgtg ttgttatatt aaataaaaaa gatatgtcta
     3481 atttaaatga gatgtcaaaa tgggaacaat tttttattga taaaggatac tatcctgtat
     3541 cagtggatgc taagcacggt aaaaatttaa agaaagtgga agctgcagca attaaggcga
     3601 ctgctgaaaa atttgaacgc gaaaaagcga aaggacttaa acctagagcg ataagagcga
     3661 tgattgttgg aattccaaat gttggtaaat ccacattaat aaataaactg gcaaagcgta
     3721 gtattgcaca gactggtaat aaaccaggtg tgaccaaaca acaacaatgg attaaagttg
     3781 gtaatgcatt acaactatta gacacaccag ggatactttg gcctaaattt gaagatgaag
     3841 aagtcggtaa gaagttgagt ttaactggtg cgataaaaga tagtattgtg cacttagatg
     3901 aagttgccat ctatggatta aactttttaa ttcaaaatga tttagcgcga ttaaagtcac
     3961 attataatat tgaagttcct gaagatgcag aaatcatagc gtggtttgat gcgataggga
     4021 aaaaacgtgg cttaattcga cgtggtaatg aaattgatta cgaagcagtc attgaactga
     4081 ttatttatga tattcgaaat gctaaaatag gaaattattg ttttgatatt tttaaagata
     4141 tgactgagga attagcaaat gacgctaaca attaaagaag ttacgcagtt gattaatgcg
     4201 gttaatacaa tagaagaatt agaaaatcat gaatgctttt tagatgagcg aaaaggtgtt
     4261 cgaaatgcca tagctaggcg cagaaaagcg ttagaaaaag aacaagcttt aaaagaaaag
     4321 tatgttgaaa tgacttactt tgaaaatgaa atattaaaag agcatcctaa tgctattatt
     4381 tgtggtattg atgaagttgg aagagggcct ttagcaggac cagtcgttgc atgcgcaaca
     4441 attttaaatt caaatcacaa ttatttgggc cttgatgact caaaaaaagt accttttacg
     4501 aaacgtctag aattaaatga agcactaaaa aatgaagtta ctgcttttgc atatggtatc
     4561 gcgacagctg aagaaataga tgaatttaat atttataaag ctactcaaat cgccatgcag
     4621 cgagctattg atggattatc agtacaacca acgcatttat tgatagacgc gatgacgctt
     4681 gataatgcac tgcctcaagt atctttaatc aagggtgatg caagaagtgt gtccattgca
     4741 gctgcaagta tcatggcaaa ggtttttcgt gatgattata tgacacagtt atctaaagat
     4801 tatcctgaat acggttttga aaaaaacgcg ggttacggta ccaaacaaca tttactagca
     4861 atcgatgata ttggcattat gaaagagcat agaaaaagct ttgaacctat aaaatcgtta
     4921 ctgtaaatcg taaaattcaa ttaaatgata aaaaagtagc atttaagttt acaatagcgc
     4981 ttacattttt tataattgta agggaactta acctaagtaa caggaggatg gaagatgaat
     5041 atccacgagt atcaaggtaa agaaatattt cgttcaatgg gcgttgcagt tccagaagga
     5101 cgagtagcat ttactgctga agaagcgctg gagaaagcaa aagaattaaa ttctgatgtt
     5161 tatgttgtaa aagcacaaat tcatgctgga ggtagaggta aagcaggcgg agtaaaaatt
     5221 gctaaatctt tatctgaggt agaaacatat gcaaaagaat tattagggaa aactttggtg
     5281 acacatcaaa ctggtccaga aggtaaagaa attaagcgtt tatatatcga agaaggttgt
     5341 gctattcaaa aagaatatta cgttggattc gttattgatc gtgcgactga ccaagtaaca
     5401 ttgatggcgt ctgaagaagg gggtactgag attgaagaag ttgctgcgaa gactcctgaa
     5461 aagatcttca aagaaactat cgatccagta atcggacttt caccattcca agcaagacga
     5521 attgcgttta atattaatat tcctaaagaa tctgttaaca aagctgctaa attcttatta
     5581 gcactttata atgtattcat tgaaaaagat tgttcaatcg tagaaatcaa cccattagtt
     5641 acaacagctg atggtgatgt attggcatta gatgctaaaa ttaattttga tgataatgca
     5701 ttattcagac ataaagatgt tgtagaatta cgtgatttag aagaagaaga tccgaaagag
     5761 attgaagcgt ctaaacatga tttatcatac attgcattag atggtgacat cggatgtatg
     5821 gttaatggtg caggtttagc catggcaaca atggatacga ttaatcattt cggtggaaac
     5881 ccagccaatt tcttagatgc aggcggaagc gctactagag aaaaagtaac tgaagcattt
     5941 aaaatcattt taggtgatga aaatgttaaa ggtatttttg taaatatttt cggtggcatt
     6001 atgaaatgtg atgttatcgc agaaggtatc gttgaagctg taaaagaagt agatttaact
     6061 ttaccactag ttgtacgctt agaaggtaca aatgttgagt taggtaaaaa aatcttaaaa
     6121 gactcaggat tagcaattga accagcagca acaatggctg aaggtgcaca aaaaattgtt
     6181 aaactagtca aagaagcata aggaaaggat gggagcacta agatgagtgt atttatagat
     6241 aagaatacta aagtaatggt acaaggtatt acagggtcta ctgccctttt ccatacaaaa
     6301 caaatgcttg attatggtac gaaaatagta gcaggtgtga cgcctggtaa aggtggtcaa
     6361 gttgttgaag gcgttcctgt tttcaacact gttgaagaag ctaaaaatga aaccggggca
     6421 acggtttcag tcatttacgt tccagcacca tttgctgcag actcaatttt agaagcagca
     6481 gatgcagact tagatatggt tatttgtatt actgaacata ttcctgtatt agacatggtt
     6541 aaagttaaac gctacttaca aggtagaaaa acacgtttag ttggtccgaa ctgtccaggt
     6601 gtgattacag cagatgaatg taaaattggt attatgcctg gctatattca caaaaaaggt
     6661 catgttggtg tagtatcacg ttcaggtaca ctaacatatg aagcagtgca ccaattgact
     6721 gaagaaggta ttggtcaaac tacagctgtt ggtattggtg gagacccagt taatggcaca
     6781 aactttattg atgttttaaa agcattcaat gaagatgacg aaacgaaagc agttgttatg
     6841 attggtgaaa tcggtggtac agctgaagaa gaagctgctg aatggattaa agcgaatatg
     6901 acaaaaccag ttgtaggctt tatcggtgga caaacagcac ctcctggaaa acgtatggga
     6961 catgctggtg caatcatttc aggtggtaaa ggtactgctg aagaaaaaat taaaacatta
     7021 aatagctgtg gtgtgaaaac agcggcaaca ccttcagaaa ttggttcaac attaattgaa
     7081 gctgctaaag aagcaggtat ttatgaagca ttattaacag ttaataaata aagttaaaag
     7141 atgatataaa tggctatagc ccattccata ctttataaaa gtatcggaaa tgggctattt
     7201 gtcgtgcgat gaattttagt ccacttttca tatttcggaa aggcaagtat cgtttttgga
     7261 caacctttca atcaaatcaa ttttcaatag gctttttgtc agtaattcgt atgttatata
     7321 tttttagtat tcaaaagttt gaagattctt tttactcctt aaattctggt aacaaataga
     7381 gcccctttgt taacgaaatg aaaaatcaaa tgtgtagata gtagtttata atctatgaca
     7441 aaggagtaat aaatatgtta aatcacattt tacttaaatt aatttggagt cgttttacta
     7501 ctgcacaaat acatctactt ttaaaaactt ttccgagttg ttgtgaagta gatgaatatg
     7561 aacgaatgca tattctagaa aatttttgta agcttttagc ggataaaaaa atagatgcta
     7621 aagttaaccg ttacaaggaa atttctgttg atgaaattga aacaacaatt cagaaaaccc
     7681 aattaaatta tataacttgt tttgatcata attatcccta tttattaaaa gaaacttaca
     7741 attatcctat cattttgttc tataaaggga atattaacct ccttagttat ccgcacactt
     7801 tagctgtcgt tgggtcacga ttatcaggcg actatacttt aaaggcattg aatcatctct
     7861 tcgtttcatt tcaacaaatg agtttttgtg tcgtttccgg gcttgctcaa ggagctgatg
     7921 caatggcaca tcaaatagct ttaaaataca atctccctac aattgcagtt ttagcctttg
     7981 gccatcaaac acattatccc aaaagtacat cagcattaag aaataaaata gaagaaaaag
     8041 gtttagttat atccgaatat ccaccacata caccaattgc taaatataga tttcctgagc
     8101 gcaatagaat tatcagcggt ttgtcaaaag gggttttaat tactgaggct aaggaacaaa
     8161 gtggcagtca catcacgata gattttgcat tagagcaaaa tagaaatgtt tatgttttac
     8221 ctggatctat gtttaatcct atgacaaaag gtaatttatt acgtatccaa gaaggtgcta
     8281 aggtagtatt aaacgctaat gatatatttg aagactacta tatttaaaac taaaaataac
     8341 gagttgcttc atatttcttt tgttaccctt tgtaatttat tttttaataa aatctataaa
     8401 aaatagacag ggaaaatgat ttgtttagat ataaaacgtt gacaaaagca aaattaagcg
     8461 tttatcattt atctttagta attagattag cgagggggaa atgacattgg cagataattt
     8521 agtcattgtt gaatcgcctg caaaagcaaa aaccattgaa aagtatttag gtaagaaata
     8581 taaagttata gcttcaatgg gacacgtcag agacttacca agaagtcaaa tgggtgtcga
     8641 cactgaagat aattacgaac caaaatatat aacaatacgc ggaaaaggtc ctgttgtaaa
     8701 agaattgaaa aaacatgcaa aaaaagcgaa aaacgtcttt ctcgcaagtg accccgaccg
     8761 tgaaggtgaa gcaattgctt ggcatttatc aaaaatttta gagcttgaag attctaaaga
     8821 aaatcgcgtt gttttcaacg aaataactaa agacgctgtt aaagaaagtt ttaaaaatcc
     8881 tagagaaatt gaaatgaact tagtcgatgc acaacaagcg cgtcgaatat tagatagatt
     8941 ggttggctat aacatctcgc cagttctatg gaaaaaagta aaaaaaggtt tgtcagcggg
     9001 tcgagttcaa tctgttgcac ttcgtttagt cattgaccgt gaaaatgaaa ttcgaaactt
     9061 taaaccagaa gaatattgga ctattgaagg agaatttaga tacaaaaaat caaaattcaa
     9121 tgctaaattc cttcattata aaaataaacc ttttaaatta aaaacgaaaa aagatgttga
     9181 gaaaattaca gctgcactag atggagatca attcgaaatt acaaacgtga ctaaaaaaga
     9241 aaaaacgcgt aatccagcaa acccatttac aacttctaca ttacaacaag aggcggcacg
     9301 taaattaaac tttaaagcaa gaaaaacaat gatggtcgca caacaattat atgaaggtat
     9361 agatttgaaa aaacaaggta cgattggttt aataacatat atgagaaccg attctacacg
     9421 tatttcagat actgccaaag ctgaagcaaa acagtatata actgataaat acggtgaatc
     9481 ttacacttct aaacgtaaag catcagggaa acaaggtgac caagatgccc atgaggctat
     9541 tagaccttca agtactatgc gtacgccaga tgatatgaag tcatttttga cgaaagacca
     9601 ataccgatta tacaaattaa tttgggaacg atttgttgct agtcaaatgg ctccagcaat
     9661 acttgataca gtctcattag acataacaca aggtgacatt aaatttagag cgaatggtca
     9721 aacaatcaag tttaaaggat ttatgacgct ttatgtagaa actaaagatg atagtgatag
     9781 cgaaaaggaa aataaactgc ctaaattaga gcaaggtgat aaagtcacag caactcaaat
     9841 tgaaccagct caacactata cacaaccacc tccaagatat actgaggcga gattagtaaa
     9901 aacactagaa gaattgaaaa ttgggcgacc atcaacttat gcaccgacaa tagatacgat
     9961 tcaaaagcgt aactatgtca aattagaaag taagcgtttt gttcctactg agttgggaga
    10021 aatagttcat gaacaagtga aagaatactt cccagagatt attgatgtgg aattcacagt
    10081 gaatatggaa acgttacttg ataagattgc agaaggtgac attacatgga gaaaagtaat
    10141 agacggtttc tttagtagct ttaaacaaga tgttgaacgt gctgaagaag agatggaaaa
    10201 gattgaaatc aaagatgagc cagccggtga agactgtgaa gtttgtggtt ctcctatggt
    10261 tataaaaatg ggacgctatg gtaagtttat ggcttgctca aacttcccgg attgtcgtaa
    10321 tacaaaagcg atagttaagt ctattggtgt taaatgtcca aaatgtaatg atggtgacgt
    10381 cgtagaaaga aaatctaaaa agaatcgtgt cttttatgga tgttcgaaat atcctgaatg
    10441 cgactttatc tcttgggata agccgattgg aagagattgt ccaaaatgta accaatatct
    10501 tgttgaaaat aaaaaaggca agacaacaca agtaatatgt tcaaattgcg attataaaga
    10561 ggcagcgcag aaataatatt tttatttcct agagacattt taagattgtt aaatagaatc
    10621 attagtgaat cttattttaa agatagtaat ggattaatct aaataagtgc ggataatata
    10681 aacataacaa cataattaaa agacataaat gacaataaaa ggagtataga aatgactcaa
    10741 actgtaaatg taataggtgc tggtcttgcc ggttcagaag cggcatatca attagctgaa
    10801 agaggaatta aagttaatct aatagagatg agacctgtta aacaaacacc agcgcaccat
    10861 actgataaat ttgcggaact tgtatgttcc aattcattac gcggaaatgc tttaactaat
    10921 ggtgtgggtg ttttaaaaga agaaatgaga agattgaatt ctataattat tgaagcggct
    10981 gataaggcac gagttccagc tggtggtgca ttagcagttg atagacacga tttttcaggt
    11041 tatattactg aaacacttaa aaatcatgaa aatatcacag ttattaatga agaaattaat
    11101 gccattccag atggatacac aattatcgca acaggaccac ttactacaga aacccttgcg
    11161 caagaaatag tggacattac tggtaaagat caactttatt tctatgatgc ggctgctcca
    11221 attattgaaa aagaatctat tgatatggat aaagtttact taaagtcccg ttatgataaa
    11281 ggtgaagctg catatttaaa ttgtcctatg actgaggatg aatttaatcg cttttatgat
    11341 gcagtattag aagctgaagt tgcgcctgta aattcatttg aaaaagaaaa atatttcgag
    11401 ggttgtatgc cttttgaagt aatggcagaa cgcggacgca agacattact atttggacca
    11461 atgaaaccag taggattaga agatccaaag actgggaaac gtccttatgc agtggttcaa
    11521 ttaagacaag atgacgctgc tggtacactc tacaatattg ttggcttcca aacgcattta
    11581 aaatggggag ctcaaaaaga agtcattaaa ttaattccag gcttagaaaa tgttgatatt
    11641 gttagatatg gtgtgatgca tagaaatacc ttcattaatt caccggacgt attaaacgag
    11701 aaatatgaat tgatttcaca accaaacata cagtttgcgg gacaaatgac tggtgttgaa
    11761 ggttatgtag aaagcgcagc tagcggctta gttgcaggca tcaatcttgc gcataaaata
    11821 ttaggcaagg gtgaggtagt atttccgaga gaaacaatga ttggaagtat ggcttactat
    11881 atttctcatg ctaaaaacaa taagaatttc caacctatga atgctaactt cgggttatta
    11941 ccatctttag aaactagaat taaagataaa aaagaacgct atgaagcaca agctaataga
    12001 gctttggatt acttagaaaa tttcaaaaaa actttataaa atagttagaa agactagata
    12061 tgctattcat tcttaagtca tcaacgagta agtaatgact ttctaaatgg aaaatactta
    12121 tcctagtctt tttaattttg gaattgttac gtatttctga caatttagca ttcgcattca
    12181 aaaaatatct gaataaataa cacgcaataa gttgattgat gtaacatgta agagaatgtt
    12241 ttaaataaac tttatttaaa agacaatgaa ataataaatg gcaaggctat taataaagac
    12301 ttttagtaat taatttaaaa aagaggtatt caaattaacg ggttttccga ttagttacaa
    12361 ttatttaatt ctcaaaagat ttagaattga ttatcaaatt actgtaagcc ctttgctgta
    12421 tatgctacaa ttcttattga tggagggtaa atgtattgaa tcatattcaa gatgcgtttt
    12481 taaatacatt gaaagttgaa cggaattttt cggaacacac attgaaatca tatcaagatg
    12541 acttaattca gtttaatcaa tttttagaac aagaacattt agagttgaat acttttgaat
    12601 acagagatgc tagaaattat ttgagctatt tatattcaaa tcatttgaaa agaacatctg
    12661 tttctcgtaa aatctcaacg ttaagaactt tctatgaata ttggatgacg cttgatgaga
    12721 acattattaa tccatttgtt caattagtac atccgaaaaa agaaaaatat cttccgcaat
    12781 tcttttacga agaagaaatg gaagcgttat tcaaaactgt agaagaggac acttcaaaaa
    12841 atttacggga tcgagttatt cttgaattgt tgtatgctac aggcatccgt gtttcggaat
    12901 tagtaaatat taaaaaacaa gatatagatt tttacgcgaa tggtgttacc gtattaggaa
    12961 aagggagcaa agagcgtttt gtaccgtttg gtgcttattg tagacaaagc atcgaaaatt
    13021 atttagaaca tttcaaacca attcagtcat gcaatcatga ttttcttatt gtaaatatga
    13081 agggtgaagc aatcactgaa cgcggtgtac gatatgtttt aaatgatatt gttaaacgaa
    13141 cagcaggcgt aagtgagatt catccccaca agctcagaca tacatttgca acgcatttat
    13201 tgaatcaagg tgcagaccta agaacagtac aatcgttatt aggtcatgtt aatttgtcaa
    13261 caactggtaa atatacacac gtatctaacc aacaattaag aaaagtgtat ctaaatgcac
    13321 atcctcgagc gaaaaaggag aatgaaacat gagtaataca acattacatg caacaacaat
    13381 ttatgctgta agacataatg ggaaagcagc tatggctgga gatggacaag taacgcttgg
    13441 tcaacaagtc atcatgaaac aaacggcaag aaaagtgcga cgtttatatg aaggtaaagt
    13501 gttagctggt ttcgcaggta gtgtagcaga tgcgtttacg ttatttgaaa aatttgaaac
    13561 aaaattacaa cagtttagtg gtaacttaga aagagctgct gttgaattgg cacaagaatg
    13621 gcgaggcgat aaacaattac gtcaattaga agctatgcta attgtaatgg ataaagatgc
    13681 tattttagtt gtcagtggaa ctggcgaagt tattgcccca gatgatgacc ttatcgctat
    13741 tggatcagga ggcaactacg cattaagcgc aggacgtgca ttgaaacgcc atgcatcgca
    13801 tttgtctgct gaagaaatgg catatgagag cttgaaagta gcggctgata tttgtgtctt
    13861 taccaacgat aatattgttg ttgaaacact ataataatca gagcacgata aataattacg
    13921 accaattaat tttagttaaa agacggagga atgaaattaa tggatacagc tggaataaga
    13981 ttaactccaa aagaaatcgt atctaaatta aatgaatata tcgttggaca aaatgatgct
    14041 aaacgtaaag tggcaattgc cctacgtaat cgatacagaa gaagtttatt agatgaggaa
    14101 tcaaagcaag aaatttcacc taaaaatatt ttgatgattg gaccaaccgg cgttggtaaa
    14161 actgaaattg caagaagaat ggccaaagtt gttggcgcac catttataaa agtagaagct
    14221 actaaattta ctgaggtagg ttatgtagga cgagatgttg aaagtatggt tagagatctt
    14281 gttgatgttt cagtaagatt agtcaaggcg cagaaaaaat cattggtaca agatgaagca
    14341 acagctaagg ccaatgaaaa acttgttaag ttattagttc caagtatgaa aaagaaagcg
    14401 tctcaaacga ataatccttt agagtcactt ttcggaggtg caattccaaa tttcggacaa
    14461 aataacgaag atgaagaaga accacctact gaggaaatta aaacaaaacg ttctgaaatt
    14521 aagagacaat tagaagaagg caaacttgaa aaagaaaagg taagaattaa agtcgagcaa
    14581 gatcctggtg ctttaggtat gctaggtaca aatcaaaatc agcaaatgca agagatgatg
    14641 aatcaattaa tgcctaaaaa gaaagttgag cgagaagttg ctgttgagac ggcaaggaaa
    14701 atcttagctg atagttatgc ggatgaacta attgatcaag aaagcgctaa ccaagaagcg
    14761 cttgaattag cagaacaaat gggtatcatc tttatagatg aaatcgacaa agttgcgacg
    14821 aataatcata atagtggtca agatgtctca agacaaggtg ttcaaagaga tattttacct
    14881 atacttgaag gtagcgttat tcaaaccaaa tatggtactg tgaatactga acatatgctg
    14941 tttataggtg ctggagcttt ccatgtatct aagccgagtg acttgatacc agaattgcaa
    15001 ggtcgttttc cgattagagt tgaacttgat agtttatcgg tagaagattt tgtaagaatt
    15061 ttgacagaac caaaattgtc attaattaaa caatatgaag cattgcttca aacagaagaa
    15121 gttactgtaa actttaccga tgaagcaatt actcgcttag ctgagattgc ctatcaagta
    15181 aatcaagata cagacaacat tggtgcacgt cgacttcata caattttaga aaagatgcta
    15241 gaagatttat cattcgaagc accaagtatg ccgaatgcag ttgtagatat taccccacaa
    15301 tatgttgatg ataaattaaa atcaatttca acaaataaag atttaagtgc atttattcta
    15361 taaaaatata caaaaggaga aaaattcatg agcttattat ctaaaacgag agagttaaac
    15421 acgttacttc aaaaacacaa aggtattgcg gttgatttta aagatgtagc acaaactatt
    15481 agtagcgtaa ctgtaacaaa tgtatttatt gtatcgcgtc gaggtaaaat tttaggatcg
    15541 agtctaaatg aattattaaa aagtcaaaga attattcaaa tgttggaaga aagacatatt
    15601 ccaagtgaat atacagaacg attaatggaa gttaaacaaa cagaatcaaa tattgatatc
    15661 gacaatgtat taacagtatt cccacctgaa aacagagaat tattcataga tagtcgtaca
    15721 actatcttcc caattttagg tggaggggaa agattaggta cattagtact tggtcgagta
    15781 catgatgatt ttaatgaaaa tgatttggta ctaggtgaat atgctgctac agttattggt
    15841 atggaaatct tacgtgagaa gcatagtgaa gtagaaaaag aagcgcgcga taaagctgct
    15901 attacaatgg caattaattc attatcttat tctgaaaaag aagcaattga acatatcttt
    15961 gaagaactag gcggtacgga aggtctatta atcgcatcaa aagttgcaga tagagttggt
    16021 attactagat ctgtaattgt aaatgcacta cgtaaattag aaagtgctgg tgtaattgaa
    16081 tcacgttctt taggaatgaa aggtactttc attaaagtta aaaaagaaaa attcttagat
    16141 gaattagaaa aaagtaaata agtcgatgag tctgggacat aattcaatgt cctagtcgtg
    16201 acgatgttat cttggcggta gtcaacgaaa tgactttgat gattatgttg aagtcatttc
    16261 tttatttttg tctaattttc aatcaaattg atatttcaca ttaacttgat agaattacct
    16321 ttaaatttat gaaaaaatgc ttgaaaaaca tttgctttag acacggcatt atgttatatt
    16381 tattttcggc tataaagcca atacacacat atcaaacgtg attgtgtaaa gggtgcaata
    16441 tttgtatatt gtctttacaa tagtttgcta tggaggtaat taaccaatag gaggaattta
    16501 taatggcagt aatttcaatg aaacaattac tagaagcggg tgttcacttc ggtcaccaaa
    16561 cacgtcgttg gaacccaaaa atgaaaaaat atatcttcac tgagagaaat ggtatttata
    16621 tcatcgactt acaaaaaaca gtgaaaaaag tagacgaggc atacaacttc ttgaaacaag
    16681 tttcagaaga tggtggacaa gtcttatttg taggaactaa aaaacaagca caagaatcag
    16741 ttaaatctga agcagaacgt gctggtcaat tctacattaa ccaaagatgg ttaggtggat
    16801 tattaactaa ctataaaacg atctcaaaac gaatcaaacg tatttctgaa attgaaaaaa
    16861 tggaagaaga tggtttattc gaagtattac ctaaaaaaga agtagtagaa cttaaaaaag
    16921 aatacgaccg tttaatcaaa ttcttaggcg gaattcgtga tatgaaatca atgcctcaag
    16981 cattattcgt agttgaccca cgtaaagagc gtaatgcaat tgctgaagct cgtaaattaa
    17041 atattcctat cgtaggtatc gttgacacta actgtgatcc tgacgaaatt gactacgtta
    17101 tcccagcaaa cgacgatgct atccgtgcgg ttaaattatt aactgctaaa atggcagatg
    17161 caatcttaga aggtcaacaa ggcgtttcta atgaagaagt agctgcagaa caaaacatcg
    17221 atttagatga aaaagaaaaa tcagaagaaa cagaagcaac tgaagaataa tcaactgttg
    17281 aatctgactt agatatagtt taaatgggtg ataagatatt aatgcttatc acctttttta
    17341 aaaagaaaat cgaggcaaat tacaaatatt caattagagt attggcaatc ttgcctataa
    17401 taatgctaaa atcataatat ataaaatgat aacttattgg aggaataatg aatggcaact
    17461 atttcagcaa aacttgttaa agaattacgt gaaaaaactg gcgcgggtat gatggattgt
    17521 aaaaaagcgc taactgaaac tgatggtgac atcgataaag cgattgatta cctacgtgaa
    17581 aaaggtattg ctaaagcagc taaaaaagca gaccgtattg cggctgaagg tttagtacat
    17641 gtagaaacta aaggtaacga cgcagttatc gttgaaatca actctgaaac agactttgtt
    17701 gctcgtaacg aaggattcca agagttagtt aaagaaatcg ctaatcaagt attagataca
    17761 aaagctgaaa ctgttgaagc tttaatggaa acaactttac caaatggtaa atcagttgat
    17821 gaaagaatta aagaagcaat ttcaacaatc ggtgaaaaat taagtgttcg tcgttttgct
    17881 atcagaacta aaactgataa cgatgctttc ggcgcttact tacacatggg tggacgcatt
    17941 ggtgtattaa cagttgttga aggttcaact gacgaagaag cagcaagaga cgttgctatg
    18001 catatcgctg caatcaaccc taaatatgtt tcttctgaac aagttagcga agaagaaatc
    18061 aaccacgaaa gagaagtttt aaaacaacaa gcattaaatg aaggtaaacc agaaaacatc
    18121 gttgaaaaaa tggtggaagg acgtttacgt aaatacttac aagaaatttg tgctgtagat
    18181 caagacttcg ttaaaaaccc tgatgtaaca gttgaagctt tcttaaaaac aaaaggtgga
    18241 aaacttgttg acttcgtacg ctatgaagta ggcgaaggta tggaaaaacg cgaagaaaac
    18301 tttgcggatg aagttaaagg acaaatgaaa taatctgtca taaagtaaaa caaggaagaa
    18361 gacaccttta atgttgcttt attaaaatgt aaatcattct aataaaacga caactgtgtc
    18421 ttctttactt gtatatgtta catatattca cgatagagag gataagaaaa tggctcaaat
    18481 ttctaaatat aaacgtgtag ttttgaaact aagtggtgaa gcgttagctg gagaaaaagg
    18541 atttggcata aatccaataa ttattaaaag tgttgctgag caagtggctg aagttgctaa
    18601 aatggactgt gaaatcgcag taatcgttgg tggcggaaac atttggagag gtaaaacggg
    18661 tagtgactta ggtatggacc gtggaactgc tgattacatg ggtatgcttg caactgtaat
    18721 gaatgcctta gcattacaag atagtttaga acaattggat tgtgatacac gagtattaac
    18781 atctattgaa atgaagcaag tggctgaacc ttatattcgt cgtcgtgcaa ttagacactt
    18841 agaaaagaaa cgcgtagtta tttttgctgc aggtattgga aacccatact tctctacaga
    18901 tactacagcg gcattacgtg ctgcagaagt tgaagcagat gttattttaa tgggcaaaaa
    18961 taatgtagat ggtgtatatt ctgcagatcc taaagtaaac aaagatgcgg taaaatatga
    19021 acatttaacg catattcaaa tgcttcaaga aggtttacaa gtaatggatt caacagcatc
    19081 ctcattctgt atggataata acattccgtt aactgttttc tctattatgg aagaaggaaa
    19141 tattaaacgt gctgttatgg gtgaaaagat aggtacgtta attacaaaat aaatttagag
    19201 gtgtaaaata atgagtgaca ttattaatga aactaaatca agaatgcaaa aatcaatcga
    19261 aagcttatca cgtgaattag ctaatatcag tgcaggaaga gctaattcaa atttattaaa
    19321 cggcgtaaca gttgattact atggtgcacc aacacctgta caacaattag caagtatcaa
    19381 tgttccagaa gcacgtttac ttgttatttc tccatacgac aaaacttctg tagctgacat
    19441 cgaaaaagcg ataatagcgg ctaacttagg tgtcaaccca acaagtgatg gtgaagtgat
    19501 acgtattgca gtacctgcct taacagaaga gcgtagaaaa gagcgcgtta aagatgttaa
    19561 gaaaattggt gaagaagcta aagtatctgt tcgaaatatt cgtcgtgata tgaatgatca
    19621 gttgaaaaaa gatgaaaaaa atggcgacat tactgaagat gagttgagaa gtggtgctga
    19681 agatgttcag aaagcaacag acaattcaat aaaagaaatt gatcaaatga ttgctgataa
    19741 agaaaaagat attatgtcag tataaaacta atatacaatg acatattaaa atgccagtat
    19801 taaacgataa tgtaacattt aaaatgggca tgtttaatta aatcaaagat gcatgtgata
    19861 atttaaattc acaatgagca taaaaatggt gtttaaacaa gttaattaaa catatacttt
    19921 ataaataata ggcattaggt atattgctat aataaagtta tgtaattttt aacctcagta
    19981 tgtatgtcac atttctggtg taaactgtac cgagtcagac tttggtacag tttttttatt
    20041 tgcttattca atgcattaaa tgagtatgat aaaatgataa tgattgttta gtaacttata
    20101 ctatatgaca gagatgatca ggctcggagg aaagaccatg tttaaaaagc taataaataa
    20161 aaagaacact ataaataatt ataatgaaga attagactcg tctaatatac ctgaacatat
    20221 cgctattatt atggatggta atgggcgatg ggctaagaag cgaaaaatgc ctagaattaa
    20281 aggccattac gaaggtatgc aaacaataaa aaaaattact aggatagcta gtgatattgg
    20341 tgttaagtac ttaactttat atgccttttc cactgaaaat tggtcaagac ctgaaagtga
    20401 agtaaattat attatgaatt tgcctgtcaa tttcttaaag acattcttac cggaactaat
    20461 tgaaaaaaat gtcaaagttg aaacaattgg atttactgat aagttgccaa aatcaacgat
    20521 agaagcaatt aataatgcta aagaaaagac agctaataat accggcttaa aattaatatt
    20581 tgcaattaat tatggtggca gagcagaact tgttcatagt attaaaaata tgtttgacga
    20641 gcttcatcaa caaggtttaa atagtgatat catagatgaa acatatataa acaatcattt
    20701 aatgacaaaa gactatcctg atccagagtt gttaattcgt acttcaggag aacaaagaat
    20761 aagtaatttc ttgatttggc aagtttcgta tagtgaattt atctttaatc aaaaattatg
    20821 gcctgacttt gacgaagatg aattaattaa atgtataaaa atttatcagt cacgtcaaag
    20881 acgctttggc ggattgagtg aggagtagta tagtatgaaa gttagaacgc tgacagctat
    20941 tattgcctta atcgtattct tgcctatctt gttaaaaggc ggccttgtgt taatgatatt
    21001 tgctaatata ttagcattga ttgcattaaa agaattgttg aatatgaata tgattaaatt
    21061 tgtttcagtt cctggtttaa ttagtgcagt tggtcttatc atcattatgt tgccacaaca
    21121 tgcagggcca tgggtacaag taattcaatt aaaaagttta attgcaatga gctttattgt
    21181 attaagttat actgtcttat ctaaaaacag atttagtttt atggatgctg cattttgctt
    21241 aatgtctgtg gcttatgtag gcattggttt tatgttcttt tatgaaacga gatcagaagg
    21301 attacattac atattatatg cctttttaat tgtttggctt acagatacag gggcttactt
    21361 atttggtaaa atgatgggca aacataagct ttggccagta ataagtccga ataagacaat
    21421 cgaaggattc ataggtggct tgttctgtag tttgatagtg cctcttgcaa tgttatattt
    21481 tgtagatttc aatatgaatg tatggatatt acttggagtg acattgattt taagtttatt
    21541 tggtcaatta ggtgatttag tggaatcagg atttaagcgt catttcggcg ttaaagactc
    21601 aggtcgtata ctacctggac acggtggtat tttagaccga tttgacagct ttatgtttgt
    21661 gttaccatta ttaaatattt tattaataca atcttaatgc tgagaacaaa tcaataaacg
    21721 taaagaggag ttgctgagat aatttaatga atctcagaac tccttttgaa aattatacgc
    21781 aatattaact ttgaaaatta gacgttatat tttgtgattt gtcagtatca tattataatg
    21841 acttatgtta cgtatacagc aatcattttt aaaataaaag aaatttataa acaatcgagg
    21901 tgtagcgagt gagctattta gttacaataa ttgcatttat tattgttttt ggtgtactag
    21961 taactgttca tgaatatggc catatgtttt ttgcgaaaag agcaggcatt atgtgtccag
    22021 aatttgcgat cggtatgggg ccaaaaattt ttagttttag aaaaaatgaa acactttaca
    22081 ctattaggtt attgcctgtt ggtggatatg ttcgtatggc aggagatggc ttagaagagc
    22141 caccagtcga gcccggtatg aacgttaaaa ttaaactgaa tgaagaaaat gaaataacac
    22201 atatcatatt agatgatcat cataagtttc aacaaattga agcgatcgaa gttaaaaaat
    22261 gtgattttaa ggatgactta ttcatagaag gtatcactgc ttatgataat gaaagacatc
    22321 attttaaaat tgctagaaag tctttctttg ttgaaaatgg tagcttagtt caaattgctc
    22381 cgagagacag acaatttgca cataaaaagc catggccgaa atttttaaca ttatttgcgg
    22441 gaccgttatt taactttata ttagctttag tcctatttat tggtcttgca tattatcaag
    22501 gcacgcctac gtctactgta gaacaagtcg cagataagta tccagctcaa caagcaggat
    22561 tacaaaaagg tgataagatc gtccaaattg gcaaatataa aatatctgaa tttgatgatg
    22621 ttgataaggc gttagataaa gttaaagata ataagacgac tgttaaattt gaacgtgatg
    22681 gtaaaacaaa gtcagttgaa ttaacaccta aaaagactga aagaaaactg actaaagtaa
    22741 gttcagagac gaagtatgtt ctcggattcc aaccagcgag tgaacgtacg ctcttcaaac
    22801 caattgtata tggatttgaa agctttttga aaggtagtac actaattttt acagctgtag
    22861 tcggtatgtt ggctagtata tttacgggcg gattctcatt tgatatgtta aatggtccgg
    22921 ttggtattta tcataacgtc gactcagttg ttaaagcggg tatcattagc ttaattggtt
    22981 acactgcgtt attaagtgta aacttaggta ttatgaattt aattcctatt cctgcactag
    23041 acggtggtcg tattttattt gttatatatg aagcgatttt cagaaaacca gttaataaaa
    23101 aagcggaaac aacgattatt gctattggtg ccattttcat ggtcgttatt atgatattag
    23161 taacgtggaa tgatattcga cgatatttct tataatttag gaggataaat aattatgaag
    23221 caatccaaag tttttatacc aacgatgcgt gatgtgccat cagaagcaga agcacaaagt
    23281 catcgtttat tattgaaatc gggtttgata aaacaaagta caagtgggat ttatagttat
    23341 ttaccgctag caacacgtgt gttaaataat attactgcaa ttgtgcgaca agaaatggaa
    23401 cgtatcgatt ctgttgaaat tttaatgcca gcgttacaac aagctgaatt atgggaagaa
    23461 tcaggacgtt ggggtgcata tggcccggaa ttaatgcgtt tacaagatag acatggaaga
    23521 caatttgcat taggtccaac acatgaagaa ttagttacat caatagtaag aaatgaattg
    23581 aaatcataca aacaattacc gatgacatta ttccaaattc aatctaaatt ccgtgatgaa
    23641 aagagaccac gttttggttt acttcgtggg cgtgaattta ttatgaaaga tgcgtattca
    23701 ttccatgctg acgaggcatc attagatcaa acgtatcaag atatgtatca agcgtatagc
    23761 cgtatttttg agagagttgg cattaatgct agaccagttg tagcagattc aggtgctata
    23821 ggcggtagcc atacacatga atttatggca ttaagtgcta tcggtgagga tacaatcgtt
    23881 tacagtaaag aaagtgacta tgctgctaat atcgaaaaag cagaagtcgt ttacgaacca
    23941 aatcataagc attctactgt gcaaccttta gaaaaaattg aaacaccaaa tgttaagact
    24001 gcacaagaat tggcagactt cttaggtaga ccagtagatg aaatcgttaa aacgatgatt
    24061 ttcaaagttg atggcgaata tattatggtt ttagtgcgtg gccatcatga aattaatgac
    24121 attaaattaa aatcttattt cggcacagat aatattgaat tagcaacaca agacgaaatt
    24181 gttaatttag ttggtgcaaa tccgggttca ctaggtcctg ttattgataa agaaatcaaa
    24241 atttatgcag ataattttgt gcaagattta aataatttag ttgtcggtgc taacgaagat
    24301 ggctatcact taattaatgt aaatgtaggt agagacttca acgttgatga atatggcgat
    24361 ttccgtttta ttttagaagg cgaaaagtta agtgatggtt caggcgttgc acattttgct
    24421 gaaggtattg aagttggtca agtattcaaa ttgggtacta agtattcaga atcaatgaat
    24481 gctacattct tagataacca aggaaaagcc caacctttaa ttatgggttg ttacggtatt
    24541 ggaatttcta gaacgctgag tgcgattgtt gaacaaaatc acgatgataa tggaattgtt
    24601 tggcctaaat cagttactcc atttgattta catttaattt ctattaatcc taagaaagat
    24661 gatcaacgag aactagcgga tgcactatat gctgaattta atactaaatt tgatgtgttg
    24721 tacgatgatc gtcaggaacg tgcgggtgtc aaatttaatg atgccgattt aattggttta
    24781 ccactgcgaa ttgttgttgg taaacgtgca tcggaaggta ttgtagaagt taaagaacgt
    24841 ttaacaggtg atagcgaaga agttcacatt gatgacttaa tgactgtcat tacaaataaa
    24901 tatgataact taaaataatt aagatcgaat gaattataag agtaggaaaa agctgaaaga
    24961 aatcagatgc ttatgtcctg ctcttattat ttttgatata atgattattc gatgaaaaat
    25021 gactgaagac atagtataat taaagataaa tttgttttaa caatattatg attagccaaa
    25081 tataaagc
//
LOCUS       DAHHYJ010000022        24898 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000022 DAHHYJ010000000
VERSION     DAHHYJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24898
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.022"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(155..1792)
                     /locus_tag="NBK22_000694"
     CDS             complement(155..1792)
                     /locus_tag="NBK22_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD2413898.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLDIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH"
     gene            1972..2571
                     /locus_tag="NBK22_000695"
     CDS             1972..2571
                     /locus_tag="NBK22_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCD2413899.1"
                     /translation="MSEKQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARSEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            2606..2715
                     /locus_tag="NBK22_000696"
                     /pseudo
     CDS             2606..2715
                     /locus_tag="NBK22_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2795..2878
                     /locus_tag="NBK22_000697"
     CDS             2795..2878
                     /locus_tag="NBK22_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413900.1"
                     /translation="MGVCPPVTMDACALLQKFDFCNNISHF"
     gene            3241..4467
                     /locus_tag="NBK22_000698"
     CDS             3241..4467
                     /locus_tag="NBK22_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2413901.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDKQRLVGLITRANVVDIVYDTIWGDSEDTVQAEHVGEDTASSKVHE
                     QHTTNVKVRDIGDDKS"
     gene            4464..5099
                     /locus_tag="NBK22_000699"
     CDS             4464..5099
                     /locus_tag="NBK22_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2413902.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIVLTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            5116..6057
                     /locus_tag="NBK22_000700"
     CDS             5116..6057
                     /locus_tag="NBK22_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2413903.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTKETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNTEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            6057..6752
                     /locus_tag="NBK22_000701"
     CDS             6057..6752
                     /locus_tag="NBK22_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2413904.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            7046..8446
                     /locus_tag="NBK22_000702"
     CDS             7046..8446
                     /locus_tag="NBK22_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2413905.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            8672..9607
                     /locus_tag="NBK22_000703"
     CDS             8672..9607
                     /locus_tag="NBK22_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCD2413906.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            9930..10781
                     /locus_tag="NBK22_000704"
     CDS             9930..10781
                     /locus_tag="NBK22_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD2413907.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDAAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGQVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(10872..12368)
                     /locus_tag="NBK22_000705"
     CDS             complement(10872..12368)
                     /locus_tag="NBK22_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCD2413908.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTSKRNQLTDVASAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTQYITDHW
                     LKPLQMKHTTFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNNTHYTTVASTLNHYKGQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALALYALPY
                     LILGINNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT"
     gene            12600..14429
                     /locus_tag="NBK22_000706"
     CDS             12600..14429
                     /locus_tag="NBK22_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD2413909.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTDL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(14569..16647)
                     /locus_tag="NBK22_000707"
     CDS             complement(14569..16647)
                     /locus_tag="NBK22_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD2413910.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVEGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVVVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFVWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITKG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIQ
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(16785..18194)
                     /locus_tag="NBK22_000708"
     CDS             complement(16785..18194)
                     /locus_tag="NBK22_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2413911.1"
                     /translation="MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            18480..19286
                     /locus_tag="NBK22_000709"
     CDS             18480..19286
                     /locus_tag="NBK22_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD2413912.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLEHLQKGKHYGATWQDKERISWGISDQNLSKIA
                     AEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVIDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(19349..20005)
                     /locus_tag="NBK22_000710"
     CDS             complement(19349..20005)
                     /locus_tag="NBK22_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCD2413913.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            20118..20591
                     /locus_tag="NBK22_000711"
     CDS             20118..20591
                     /locus_tag="NBK22_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD2413914.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNGDLAGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(20714..21925)
                     /locus_tag="NBK22_000712"
     CDS             complement(20714..21925)
                     /locus_tag="NBK22_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCD2413915.1"
                     /translation="MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQL
                     PLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(22107..22766)
                     /locus_tag="NBK22_000713"
     CDS             complement(22107..22766)
                     /locus_tag="NBK22_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413916.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(22826..23968)
                     /locus_tag="NBK22_000714"
     CDS             complement(22826..23968)
                     /locus_tag="NBK22_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD2413917.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIINDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            24226..24612
                     /gene="gtxA"
                     /locus_tag="NBK22_000715"
     CDS             24226..24612
                     /gene="gtxA"
                     /locus_tag="NBK22_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCD2413918.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLFHIEYMISHI
                     TGFIVAFVISYYLNCYFVYRVKPTWRKFISFPITQIVNVSLQTVLLYVFVSWLNLPAE
                     FAPFAGLVITIPITFVLSKWILKDSN"
     gene            24746..24853
                     /locus_tag="NBK22_000716"
     CDS             24746..24853
                     /locus_tag="NBK22_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD2413919.1"
                     /translation="MFNLLIDIMTSALSGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT         8309 a         3732 c         4691 g         8166 t
ORIGIN      
        1 gagcatagaa cattgattta tgtcccagcc tgctcatcat cttgttatgt tatttataac
       61 ccattttcaa aataaaaaac caaccaagat gttgaaatca atggttggtt aatgtgtgtg
      121 ttgaaatcat atatggcact gcattttaaa atatttaatg agatgacttt gattcattat
      181 ttagtgattc gcgcatcttc gtattgattc gtgttgcgcg acggaaatag acgctttcaa
      241 atgaactcgt tgtaccgatg taatagaata taagcgccac aataataata acgataaagt
      301 caaatggata gtgaatcata tttaagcctt taaattcttt gcttcctata aatgacagta
      361 ttgatagcac gattaaatat acaataatcc ataagctacc accaatttgt ttctttgtat
      421 tacgccaatt catacgatat tcatagaaga agtagattgg taatccaagt ataatgatta
      481 aaataacttc agccgttgtt ggccacattg cccaatatat gtctaatgaa gctaatacaa
      541 atgataatgg tgccattact tttaaaattt ttgctctaaa tggacgagtc attgttggtc
      601 ccatttttct taatgcaatc actgtcgttg ggccagttaa ataggctact aaagttgcag
      661 tagaaataac tgctgctagc gtaccccaat ctctaaataa tgtaaccata atcatactaa
      721 tgatggcatt aaagatgatt gctacacgtg ggatatgata tttttcattc atcttcccaa
      781 gaaatttagg gatatgtcca tttttctcca ttgctcgtaa aactcgacct gtaacggcga
      841 caaatgacac gccagtaccg aatggtgata caaaagcttc aatatatagt aaaattgcga
      901 gccaattaat tcctaataag atagctaaat cagcaaatgg tgaattgaag ttgatgccat
      961 tccatccact atgttgtaac attgattgag gcatagaagt gataaacgta ctttgtaaaa
     1021 tgatgtacaa cactgcactg attgacagtg aaatagcgat gcctcttgcg atattttttt
     1081 caggattttt aatttctgaa cccatattaa taattgtctg gaatgaattg aatgaaaaaa
     1141 taatccctga tgctgttgtt gcagcaaaaa tcggtgcact tccgtaaggc ataaatgtgc
     1201 ttgccgaatg gccataattt gaagtgtcga atcctgataa catcaacatg atgatggtta
     1261 acatgggtac gcctaattta aatacagaaa ttaaactcgt aaatgatgtt aaaagtttta
     1321 cggaccaata gtttaataat gaaaaaataa caatgatgag atatacagct agcaatccgt
     1381 atgtgctaat agagccattt tccattaaat atctcattgg tttcgcccaa tgccacggcc
     1441 aagaactcat atattgaaca gctgacacag cttcgatagg tattattgtc accaaagaca
     1501 cccaattcgc ccaagcagca ataaagccta ataatgagcc atgtgtatac tgggcatagt
     1561 tactcatgcc acctgattga ggaaacattg tgccgatttc aatgtagtta taggcaatgg
     1621 ttccaatgac taggaatcca agaacccatg atatgattgc tgctggtcca gctattgatg
     1681 atgcttccca cgcaccaaat agccaaccag atcctattaa agagcctaac cctaataaga
     1741 ctaattggga tagattgatt ttgtcgttgt gcaattgctt gtctttattc ataaatactc
     1801 cttcgttatt gccaaaaata caaaaaataa tatgcgacaa ttatacgctt attataataa
     1861 tagtcaaatg cattttttgt aagaacgcaa aagcatttgt aaaattagtt ttttataaaa
     1921 ttaggatata taatacaatg tgtattgaaa aacaaaatgg aagtgatgac tatgagtgag
     1981 aaacaatcaa atgaacaagt tgaagcattt ttaaataaag agagtcaatg gcaagaagaa
     2041 tataaatatt tgagagcgtt aatctttaat gaaacagaat tagaggaagc gtataaatgg
     2101 atgcatcctt gttacacgtt gaataataaa aatgtagtac ttatccatgg cttcaaaaat
     2161 tatgttgcac tattatttca taaaggtgcc attttggagg ataaatatca tacactcatt
     2221 caacagactg aaaaggtgca agcagctcgt cagttacgat ttgaaaattt aacagagatt
     2281 caagcacgta gcgaagaaat taaatattat ctagctgaag caattaaagc tgaaaaagct
     2341 ggtaaaaaag ttgaaatgaa gaaaacagag gaatatgtta ttcctaaaga gttagaagct
     2401 aagttcgaag aaatgccaca gctagagtcg tcattttata aattaacgcc aggcagacaa
     2461 catcaatata tatatcatat tgggcaagct aaacgcagtg aaacaagaca aaagcgtgtt
     2521 gaaaagtata ttaaccaaat actagaaggt aaagggatgc atgataagta attaatgagt
     2581 aaaagcatac cggttataca acaacataca agatgacacg aaacaaccaa tggctcatgc
     2641 tgttggttgt tttttaggtg tgcatgtcac gggcaacact ttgacggtgg aattccgtta
     2701 cagacttggg agtaggaact gttagcaaaa ggcaagggtg tctacaatga atgatgaaga
     2761 tattaaaata taaggatgac tttgtgagtg gcggatgggc gtttgtccgc ctgtaacaat
     2821 ggatgcgtgt gcattattac aaaaattcga cttttgcaat aatatttcac atttttgaca
     2881 cttttttgct ataaaacaac caattgagcg acaataaatt cgcttttaaa aaatatgagt
     2941 tatctattta gttgccaaag ataaaataat aatgtttaat aacatcatat agagtatgtt
     3001 agttttaaat gtcgaatata cgaatgtgca aacaaagtaa tcggtagaaa ttcaacatac
     3061 atagcgccgt ttactgttaa gtattcacat tacagatgaa aaatataaaa ttctacataa
     3121 tcaagaccat gatgtgtact tgtttaactt atgactctat ttgtttaaca attgcgatga
     3181 tggtcttttt attttatgcg tatcattcgt catatttttt atgaggaagg agaaatgatt
     3241 atgttaagta ttaagcattt aacgaaaatt tattctggta ataaaaaggc agtagatgac
     3301 atctctttag atattcaatc tggggaattt atcgcattta ttgggaccag tggaagtggt
     3361 aaaacaactg ctttaagaat gataaaccgt atgattgaag cgacagaagg acaaattgaa
     3421 attgatggta aagatgttcg gagtatgaat cctgtcgaat tacgtagaaa tattggttat
     3481 gttattcaac aaattgggtt aatgcctcat atgacgatta aagagaatat tgtattggta
     3541 cccaaattgt tgaaatggac taaagaagaa aaggataaac gtgcaaagga attaattaaa
     3601 cttgtggatt taccggagtc atttttagag cgttatccag cagaactatc aggtgggcaa
     3661 caacaacgta tcggtgtcgt aagagcactt gcggccgaac aagatattat tttaatggat
     3721 gaaccttttg gtgcattgga tcctattacg agagatacgt tacaagattt agttaaaacg
     3781 ttacaacgaa aattaggcaa gacgtttatc tttgtaacac atgatatgga tgaagcgatt
     3841 aaattagcag acaaaatttg tattatgtca gaaggtaagg tggtgcaatt tgatacacca
     3901 gacaatattt taagacatcc cgcaaatgat tttgtacgtg attttatagg acaaaataga
     3961 ctgattcaag accgtcccaa tgacaagact gtagaaggtg taatgattaa accaatcacg
     4021 atacaggcag aagcaacact gaatgacgcc gttcatatta tgagacaaaa acgtgttgat
     4081 actatttttg tagtagatag taataaccat ttactaggtt tcttagacat tgaagatata
     4141 aatcagggta tacgtggaca caaaagttta cgagacacca tgcaacaaca tatttatacc
     4201 gttcaaattg attctaaatt acaagattct gtacgtacga ttttaaaaag aaacgttagg
     4261 aatgtacctg tcgtagatga taagcaacgt ttagtaggac tgattacgcg tgccaatgtt
     4321 gttgacattg tttatgacac gatttggggc gatagtgagg atacagtgca agcagaacat
     4381 gtgggggaag acactgcgtc ctcaaaagtg catgagcaac acactactaa tgtcaaagta
     4441 cgtgacatag gagatgataa atcatgattg agttcctaca tgaacatggt ggacagttga
     4501 tgtcgaaaac actggaacat ttttatattt ctatagtggc attattactt gccatcattg
     4561 ttgcagtacc tataggcatt ttattatcaa aaacaaagcg aactgccaat attgtattaa
     4621 ctgtggcagg tgtcttacaa actattccaa cactagctgt acttgctatt atgataccga
     4681 tttttggtgt tggtaaaacg cctgcaattg tagcgctatt tatttatgta ttattaccta
     4741 ttttaaataa cacggtactc ggtgttcaaa atattgatag caacattaaa gaagctggaa
     4801 aaagtatggg aatgacacaa tttcaattga tgaaggatgt tgaattgccg ttagcattgc
     4861 cgcttatcat tggtggcatt cgtttgtcat ctgtgtatgt aattagttgg gctacacttg
     4921 caagttatgt aggtgcgggt ggattaggtg atttcatttt caatggttta aatttatatg
     4981 atccactgat gattgtaact gcaacggtac tcgttactgc actagcatta ggtgttgatg
     5041 ccttattagc tttagttgaa aaatgggtag ttcccaaagg cttaaaagta tctggataat
     5101 taggaggcta agataatgaa gaaaattaaa tatatacttg tcgtgtttgt cttatcgctt
     5161 accgtattat ctggatgtag tttgcccgga ctaggtagta agagcacgaa aaatgatgtc
     5221 aaaattacag cattatcaac aagcgaatcg caaattattt cacatatgtt acggttgtta
     5281 atagagcatg atacacatgg taagataaag ccaacattag taaataattt agggtcaagt
     5341 acgattcaac ataatgcctt aattaatggg gatgctaata tatcaggtgt tagatataat
     5401 ggcacagatt taacgggagc tttgaaggaa gcaccaatta aagatcctaa gaaagcaatg
     5461 atagccacac aacaaggatt taaaaagaaa tttgatcaaa cgttttttga ttcgtatggt
     5521 tttgcgaata cgtatgcatt catggtaacg aaggaaactg ctaaaaaata tcatttagag
     5581 acagtttcag atttagcaaa gcatagtaaa gatttacgtt taggtatgga tagttcatgg
     5641 atgaatcgta aaggcgatgg ctatgaagga tttaaaaaag agtatggttt tgactttggt
     5701 acagtgagac caatgcaaat aggtctagtc tacgacgcat taaacacaga gaagttagac
     5761 gttgcattag gttattctac agatggtcga attgccgcgt atgatttgaa agtacttaaa
     5821 gatgataaac aatttttccc accttatgct gcgagtgctg ttgcaacaaa tgaattatta
     5881 cggcaacacc cagaacttaa aacgacgatt aataagttga caggaaagat ttcgacttca
     5941 gagatgcaac gcttgaatta tgaagcggat ggtaaaggta aagaacctgc tgtcgtcgca
     6001 gaagaatttt tgaaaaaaca ccactatttt gataaacaga aaggtggtca taagtaatgg
     6061 aaggtaattt attacagcaa ttattcaatt attatgttac gaactttggt tatctatggg
     6121 atttattttt caaacactta ttaatgtctg tctatggtgt gctgtttgca gctttaattg
     6181 gtattccatt gggaatcttg cttgcaagat acacaaaact ttctggattt gtaattacaa
     6241 ttgcaaatat aattcaaaca gttccagtca ttgcaatgtt agctatttta atgttagtca
     6301 tgggcttagg ttcagaaaca gtagttttaa cagtgttttt atatgcgtta cttccaatta
     6361 taaaaaacac ttatactggt atagctagtg ttgatgcgaa tattaaggat gctggcaaag
     6421 gtatgggaat gacacgcaat caagtgctac gaatgattga attaccgtta tctgtttcgg
     6481 ttattatcgg tggcattcgt attgccttgg ttgttgcgat aggtgttgtt gccgttggat
     6541 catttatagg agcacctacg cttggtgaca ttgtgattcg tggtacaaat gcgacggatg
     6601 gcacaacgtt tattttagca ggtgcgattc cgattgctat cattgcaatc gtcattgatg
     6661 tactattaag atttttagaa aaacgattag acccaacaac acgacatcgt aaaaatcaat
     6721 ctaatcatcg gccgcaaagt attaatatgt aatagtagaa gatgtttata atttagcgat
     6781 ttcgtttcat gatttataaa aaatgaggct actcaaggag ctcaaataat ctttgagtag
     6841 cctttttata ggttgtgttt gtatgcgttt acactaaaat agcaattatt atcatgaaag
     6901 tttttggata aaaagcgtta attattgtaa aaatgctaaa aaatgagatg ttttatttat
     6961 aattttctgc aaatttatga tattgtttct taatatatca tattaaaaat ttgtttttct
     7021 taaacatagg aggcttatct aattcatgga cacatcaaaa caatttagag gtgacaaccg
     7081 attgcttttg ggtatcgttt taggggttat taccttttgg ctattcgcgc agtcacttgt
     7141 taatcttgtt gtcccattac aatcaacata tagtagtgac gttggaacga taaatatcgc
     7201 tgttagctta tctgccttat ttgctggttt gtttatcgta ggtgctggtg atgttgctga
     7261 taaatttggt cgcgtcaaaa ttacttatgt aggattgata ttaaatgttg taggttcatt
     7321 actcatcatc attacacctt tgccagcatt tttaattata ggtagaataa ttcaaggttt
     7381 gtctgcagca tgtattatgc catccacact tgctattatt aacgaatatt atattggtac
     7441 aagaagacaa cgtgccttaa gctattggtc tattggttct tggggtggta gtggtatttg
     7501 tacgttgttt ggtggcttaa tggctacata tataggttgg cgttcaatat ttgttgtttc
     7561 aattctatta acattattag caatgtactt aatcaaacat gcacctgaga ctaaagcaga
     7621 accaatcaaa ggtatgaaag cagaagctaa aaagtttgac gttattggtt tagtcatttt
     7681 agtagtgacg atgttaagtt taaatgtaat catcacacag acgtctcatt ttggtttagt
     7741 ttcaccgtta attctaggtt taattgttgt gtttatctgt tcattagttg gttttgtata
     7801 ttacgaaaat aaaataaagc atccacttgt tgatttttca atttttaaaa atagaggata
     7861 cagtggtgca actatttcaa acttcttatt aaatggtgta gcaggtggtg cacttatcgt
     7921 tattaacacg tattatcaac aacaattagg atttaattct tcgcaaacgg gttatatttc
     7981 attaacgtat ttaatagcag tgttgtcaat gattcgtgta ggtgaaaaga ttttatctca
     8041 acatggtccg aagcgcccac tattactagg aagtggcttt acagtgattg ggttaatctt
     8101 attgtcgtta acatttttac cagaagtgtg gtatatcata tctagtatag ttggatattt
     8161 attgtttggt actggtttag gattatatgc tacaccatca actgatacag cagttgctag
     8221 tgcgccagat gataagtcgg gtgttgcttc aggcgtatat aaaatggcgt catcattagg
     8281 aaatgcattt ggagtagcag tatctggtac agtttatact gtgttagcag ctaatttaaa
     8341 tttgaactta ggtggtttca caggtatgat gtttaatgcc ttgctagcaa ttgttgcatt
     8401 tttagtcatt ttactattag ttcctaaaaa tcaaacgaat ttgtaaaacc gaaatgaaag
     8461 caagttatta tgtagggatt ttaaaggaaa ttttgtgaaa gtaagtttat catacacact
     8521 taatgttacg tattgacgtt taatgttagg tgtgttcttt tatagacgat aaaagctgtg
     8581 tgcatattaa gcgaatgatt ttcaaattga cgctaatatg cgaaagtagt atttttaaaa
     8641 tgaacaacaa cgatgaagag gggtttatag gatgaaaatt gcaattgctg gatcgggtgc
     8701 attaggtagt ggctttggtg ccaaactatt tcaagcagga tatgatgtca cacttattga
     8761 cggatataca tctcatgttg aagcggttaa gcaacatgga ttaaatataa cgattaatgg
     8821 agaggcattc gagttaaaca ttccgatgta tcattttaat gatcaaccgg acgaaagcat
     8881 ttacgatgtt gtctttctat ttccaaagtc tatgcaatta aaagaagtga tggaagatat
     8941 gaagccacat attgataatg aaacgatcgt cgtatgtacg atgaatggtc tgaagcatga
     9001 agaagtcatt gcgcagtatg ttgctcaatc acaaattgtc agaggtgtta cgacttggac
     9061 ggcaggtctt gaaagccctg gacacagtca tttacttggt agtggaccag ttgaaatagg
     9121 tgaactagtg gatgaaggta aagaaaatgt tataaaagtt gctgatttac ttaacgaagc
     9181 ggaattgaat ggtgtcatta gtaaagattt ataccaatcg atttggaaaa agatttgtgt
     9241 taatggtacg gcaaatgcat taagcacagt attggagtgt aatatggcat cactgaatga
     9301 aagtagttat gcgaagtgtt tgatttataa attaacgcaa gaaatagtgc atgtagcgac
     9361 gattgataat gttcatttaa atgttgatga agtatttgaa tatttagttg atttaaatga
     9421 aaaagttggt gcgcattatc catccatgta tcaagattta attgttaata atagaaaaac
     9481 tgaaattgat tatattaatg gcgcagttgc aacattaggt aaacaacgtc atattgaagc
     9541 gccagtcaat cgctttatta ctgatttaat tcatactaaa gaaagtcagc gacatgcaca
     9601 ggattaatta gtgtttttag gtatgattac aaggaaatga caatcacgtg atattacggt
     9661 cattattaag attgaaatgt aataaataaa gaacagcagt aaggtacttt caaattgaaa
     9721 tgatcttggt gctgtttttc ttgattgatc ttcgtcataa ttcagatttg tcataggtct
     9781 acgacatact attagcattt actagacagt ttttacgacg acactttgaa aaattttgag
     9841 gcaaatcatt tggaagtctc acgtgaattt tgtaaactca tcaagcaagt aattatatta
     9901 aaaagacaaa tagagaaaag gtgtttataa tgagtaaaat ttttgtaact ggtgcaacgg
     9961 gccttattgg cattaaatta gttcaaagac taaaagaaga ggggcatgag gttgctggtt
    10021 ttactacatc tgagaatggt caacaaaagc tagctgcggt taatgtaaaa gcatatattg
    10081 gtgatatatt aaaagctgat accattgatc aagcgttagc agattttaaa ccagaaatca
    10141 ttatcaacca aattacggat ttaaaaaatg ttgatatggc agcaaatacg aaagtacgta
    10201 ttgaaggttc taaaaactta attgatgcgg cgaaaaagca tgatgttaag aaagtaattg
    10261 cccaaagtat tgcctttatg tatgaacctg gcgaaggatt agcaaatgag gaaacttcac
    10321 ttgattttaa ctcaactggt gatagaaaag taacggttga tggtgtggtt ggtctagaag
    10381 aagaaacggc tcgtatggat gaatacgttg ttttacgttt tggctggtta tatggcccag
    10441 gtacttggta cggaaaagat ggcatgattt ataatcaatt tatggatggt caagtgacac
    10501 tttcagatgg cgtaacatca tttgtgcatc ttgatgatgc agttgaaaca tctattcaag
    10561 ctattcattt tgaaaatggt atctataatg tagcagatga tgcacctgtt aaaggttctg
    10621 aatttgcaga atggtataaa gaacaacttg gtgttgaacc aaatattgat attcaacctg
    10681 cgcaaccatt tgaacgtggc gtaagcaatg agaagtttaa agcgcaaggt ggtactctga
    10741 tttataaaac ttggaaagat ggcatgaatc caattaaata ataatttatc cgtttaatat
    10801 acaaagaata aagacttggt cgaatcgtgg atgatatatt atcaaacgca cggctcgaac
    10861 aagtcttttt attatgtctt cgttatcttt gtatgaagga ataacagaat tacaattaat
    10921 gtactgaata atgcaattaa tgttgtgatt agtgctaatt taatttctat tggtagccaa
    10981 gtcagtacaa aagaccaatt attgataccg agaattaaat atggtaatgc ataaagcgcg
    11041 agcgctaaag cgatacatat acataatgat aaccaactca atacagcaat ccgttttgat
    11101 ctacgcaaaa gaatttgacc atggcgcatt tgccaagcac gataagctga gaatataaat
    11161 gctaataaaa taagtgttgt catcaaaacg gtaacaatat tgaattgtcc tttataatga
    11221 ttcagcgttg aagcaacagt agtatagtgt gtgttgttaa ctatctgtgt attaagatgc
    11281 tcaactaatt tgggtacata ttccgaattt agatttgcaa gtacaacaat tccataattt
    11341 tgttttggat ttagtaaaat aaatgatgaa aagttatcga gcgttcctga atgaaacact
    11401 aaatgttcat cattattggt aaaccagccc gaagcatatg cattggcatt aggttcacca
    11461 attgttgatg ataaattttt atgtgattgt tgaactaatg atttgtattt atcaggtgga
    11521 ttaagttgga attttatcca atgttccaaa tcttcagttg atgtcatcat atatgctgat
    11581 ggtgtatccc aaaggttaaa ttcaggttta gagacgacag gtgtcgaacc ttgtaattca
    11641 tagccaatag catcatgttt tgatttgtaa tttgtttgtt taaatgtcgt gtgtttcatt
    11701 tgaagtggtt taagccaatg gtctgttata tattgcgtat atgattgctt tgtgacattt
    11761 tggatgatta aacctaacag atcataattc atatttgagt attcaaattc ctcgcctggc
    11821 ttatggtgaa gctcatctcc cataattgca cttgccacat ctgttaattg atttcgcttg
    11881 cttgtcacag aatcttcgct tgtaatatca ctaggtatac cacttgtttg agccaaaagt
    11941 tgcttaatcg taatagtttc attttgacca ttatagttca ttttaaaatg aggcacatgt
    12001 ttggatacgg catcatttaa gtttaatcga ccttcttgag ctaattttaa aattgcaaga
    12061 cctgtgaaag ctttcgtatt agaagcaatt tcatactttg ttgtgggaga agctttgact
    12121 tttttatcaa catcttgata accataacct ttatttaaaa aaactttgcc attttttact
    12181 attaaaattg atgctccagg aatgtgtccc ttttgtaaat catgctcgat aattgtatct
    12241 atttgttgtt gcgaatcatt ggttaaccgt gtcttcgtat tgctatttaa tgttatatat
    12301 atagcaattg aaatggcgac taaaatgata atagaaatgg atagaaaata cagtttttta
    12361 gtagtcataa acgttccttt ttatatgtta aatttactta agtgcctaat gagatttatt
    12421 ttattatacc agttctattg aataaattaa ttaaagaggt atatttacca ttatttttga
    12481 caccttatat gttaataggt gtataattac ataaaaaata ttaattaact ctctatactt
    12541 tttggaaaga taggcgtata gtgatatcca tcaaaaacta taaaaaggaa taggagatta
    12601 tgttcaatca atttaaaaga cttattatag ggcaacctaa aaaaaacaga gaactaaaag
    12661 acgaaaagat atcgaagttt aaagggttag cgattctttc gtcggatgca ttatcttcag
    12721 tggcttacgg tcccgaacaa atactgatta cactatcagt agtgggtgca gttgcaactt
    12781 ggtatacttt accgattgca ggtgctgttc tgatcttatt agctgcttta attatgtctt
    12841 ataggcaaat tatttatgca tatcctaaag gtggcggcgc gtatatggtg tccaagaccg
    12901 atttaggaga aaaatgggga ttgcttgcag gaggctcatt attagtcgat tacatactga
    12961 ctgtcgctgt tagtatatca tctggtgctg atgcatttgt tgctgcattc ccaagtttat
    13021 atgggcataa agtactgatt gcatgtttac ttgtactttt tattttaata ttaaatttac
    13081 gtggtttaac tgaatctgcc accgtattat cctatccagt atatcttttc attatagggt
    13141 tagtgatatt aatatttata ggtactttcc gtgtggcgac aggagatatt caaccacata
    13201 tgcatgcatc agtaggaact gcggttcctg gagtaacatt attcttgcta ttaaaagcat
    13261 tttcatcagg ggcttcatca ttaacaggtg ttgaagctat ttcaaatgcg gtaaccaatt
    13321 ttagagaacc aagtgctaat aatgctgtga aaacgttaat tgctatgggt tcaattttag
    13381 cgtttttatt agttggtatc gttggtttag catatgttta tggaattatg ccacaaacgg
    13441 aaacgacagt tttatcacaa ttagctatgc aaatatttgg tgataatgct gccttctatt
    13501 ttgtacaggc aacgaccgtg atgattttgg tattagcagc caacactgga tttacagctt
    13561 ttccgatgtt agcggcttca atgtctaaag ataaatatat gcctagaatg tttactgtta
    13621 gaggtgatcg cttaggctac tctaactcaa ttattatttt aggtgtatta gccattattt
    13681 taattattgt atttgatggt atgactgaag atttaattcc attgtatgcg gttggtgtat
    13741 tcattccatt tacactagca caattcggta tggttattaa atggattcat gaacgtccta
    13801 agaattggtt aagtaagttg tctgtgaact tactaggtgg tatcgtgaca tttattgttt
    13861 ttatgatttt actcataact aaatttagcc aagtatggcc gatacttatt ttcttgccgt
    13921 tcgtggtgat tttcttcttg aaaattaaca aacattatcg agatatcgca gaacaattac
    13981 gttctgacat tgatgttctt aatgtggacg tggtagatcg taatttagca attgtgccga
    14041 taacaagtat tacaacagca gttgataagt ctatttatta cgcacaaatg cttgcgaata
    14101 atgatgtcat agcagtacat gtatcatttg gagatgaaga tgaaaaagca ttccaagaga
    14161 aatggaaacg tcatttccca gatgtaagat tagttatttt acactcggaa tatcgcagta
    14221 ttatacgacc aatttcacgt tttatcgata agattaatcg taaagccaac gatcaaaact
    14281 atatgattac agtagtgata ccagaattta ttactaagaa acgttggcat aatttgttac
    14341 acaatcaaac tagtttgcgt atgaaattgt atttaattta tcaaaagaat gttaacgtat
    14401 gtacaattcc atttaaactt aaaaaataga ttctataatc aaatcggatt gatgaaaggt
    14461 tatttacttt tcatctatcc gattttttga tgttgaatat aaaaaacccg tacatatgag
    14521 atgataatga ctttgtctca tatgtacggg ggtgttgtta tgtcttgctt aatgtttgga
    14581 ctgtaaagac aagacaattt cgttgtaatt gatattttct cgaatttgag ctgcaacgat
    14641 attgttgatt ttatttttgc gaattaattc atctaaaaga tgacgttgta agtacaatgc
    14701 ttccaatttg tggttatgga acatagcttg cggtatgatt tgattgctgt tttcttgacg
    14761 ttcttttcga cgttgtgcac gccactttaa tgagcgactc atatcatagt aatgattaat
    14821 gactaagcta acttcaagta catttgtact attttgttct tcttttaagc gtaaaataat
    14881 attgtgattc acaatacgca taattttttg aacatctttg aatgaatttt caaattcttg
    14941 cttcttagat tctttgttta aggctttgta gtgcttcatc gctttaagtc taagccataa
    15001 catcttgaag aagtttgcaa atttttcaac aaatgaagca gtacggtgtg tctctgttaa
    15061 ttcgacaata ttgcggtaat tatttatatc ctgatatgtt gccttacctt tatcaattaa
    15121 acgctcaagt gtagatgttt cgattacttt tgcaatatct tctaaacgtt tgagttcttt
    15181 tgtattttga ttatcaggtt ctgaatttaa taaaaataac aattctccat agtattggtt
    15241 taatactggg cgataatttg tatcgttttt gtctttttta ctttcgtttt taaaatgctg
    15301 gatcactttt tgaacaatga aaattttggc agattgataa gtcatacctt taaaagtagt
    15361 atcttcggca gatggtgtaa ttaaaggtaa aacaatttgc gctaagatta aactaattaa
    15421 taccatgaaa gatgcaataa acaataaatc attacggtat tcgaatgctt gtccttttgt
    15481 aatgataaat ggtaatgtaa gtgccattga aagtgaaatt gtaccgtgaa taccacacat
    15541 ggtcataata aatgcgtaac gcactcgaga aggtggtgtt tcatgtgaat catgttcctc
    15601 gtctagataa gattgtatat ttttcgggaa ataaaaatct ttgtaccaga aataaaccca
    15661 aacaaatcga catgcataaa ttgcaatagc gattaatata gtgatcacta ttaaaaatga
    15721 aaagttgtca ggctctgttt ggaaaatatc tatgactaca gtaggaatca taaaacctaa
    15781 tacaacaaat acaaaaccat ttaaggcata actaaatgtg ttccaaattt gatggtaatt
    15841 catttgtagt tccgtttgtg ctctaattaa acggtcgcgt tctaaaccat ggattagacc
    15901 tgcgattaca actgcaatga tacctgaagc atgaacttct tctgctaaaa agtatacgac
    15961 aaaaggagtt aataattgaa taaaagttaa ggtattgtta tcttttaaac ctttattagc
    16021 ggttaagtct atacgtattc taacgacaac gaatccaata attgcaccaa taagtacacc
    16081 tagtattgtt gaaatgataa attgttcaac agcttggaat aatgaaaagg taccagttac
    16141 taatgcagta acagcaattt taaatgaaat gatacctgct gcatcattga gtaaagattc
    16201 accttctaaa atcgtcatag aacctttagg taataatttt ccgcgtgtaa tagcagatac
    16261 tgctactgca tcagtaggac ataaaattgc tgctattgca aaagcggctg gcattggtaa
    16321 ggcaggccaa atccaatgta taaaatagcc aacaccgact acagttgcaa acactagtgc
    16381 cattgacatt aatagtatag gtttacgata ttctaataat tttgttcgag agacgtgggt
    16441 accttccaca aaaagtagtg gcgcgataac ggcaaacata aatacttcag aattgaattg
    16501 gaaatcaact tgtattggaa taatgaaaat aacgacacct aatgcaattt gaataaaggc
    16561 agtaggaatt tgtgggaatc gattattgat aaccgaacta ataatcacag caaaaataaa
    16621 aattaaaaat gcttctaata gtgccataca atactcctca aaattttaat agttaatatt
    16681 ttatcacttt taaggcataa tgacatagat atattgataa aatgaagtta ttttcaaaaa
    16741 aactctagta tcggttgaac tgatactaga gcgagatgtt taaattattg attgtcatat
    16801 ctgaaatgac cgctgtcatt ttgtcgttgt tcatacgcga gcttttcagc attcgttttg
    16861 tattttttat aaaagaaaaa taaaaatata aaccagaatg gcgaaatata aatagctgct
    16921 cttgtttcgt cactaaagaa taataaaatg aatacaaaga agaagaacgc tagaataatg
    16981 taagcaatag gcttaccacc aatcaactta aatttactgt ttttatgtgc ctcaggatgc
    17041 tttttcaaat acattagata agcgacaata atcattgccc aaacaactaa aaacaacaca
    17101 gttgataacg ttgtaacgta tatgaatagt tgaattgcat tagggaaaat gtagtttaac
    17161 aaggctgcta taagtaatgc aattgatgaa actaacattg ataaataagg cacgccatga
    17221 ctattcgttt tatttaaaac tttaggacct aacccttgtt gtgacagtcc gaataagata
    17281 cgactattcg aatagatacc actatttgta gcagaggccg cggcagttag cacgacaaag
    17341 ttaacgacac ctgctgcaaa tggtacgccg attaatgtaa agagtttaac gaaagggcta
    17401 ctatttggat cgatatcatt ccaaggtatg actgacataa ttactaatag accaccgata
    17461 tagaataata aaatacggat aggtacatta ttaattgctt tcggtaaggt tttttcagga
    17521 tctttcgttt caccagcagt tacacctata agttcaatac caatgaatga atatacagca
    17581 atttggaatg acattaagaa accaaatgtt ccaccaggga acatgccacc gtgactgatt
    17641 aagtttgtga atgatgcatg tccataatgt gttttaaatg agaaaaagat taatacaaga
    17701 ccaacgataa ccatcgcaat aattgtgaca actttaataa tcgagaacca aaactccagt
    17761 tcaccaaata atctggcacc taataagttg aagctaatca agattaaaac aataaatagc
    17821 acggtaatcc aatttgggac ttgtggatac caaaatgcaa agtattgtcc catcgcagtt
    17881 aggtctgaca tacttgatac aatccaacat aaccagtaag tccagccaat gacaaagcca
    17941 ccaaaagggc ctaaatattc atttgcaata tcaacaaatg aattaaatct tgtattgctc
    18001 aacaacaatt cgcctaatgc gcgcataaaa gcgaatagta caaccccaat aatcatgtat
    18061 gtgaataaca gtgatggacc agttaaagat attgtttgac ctgatcctag gaataaccca
    18121 gttccaattg cgcccccaat tgctatcaat tggatgtgtc tgttattcaa ctctctatgc
    18181 aattttcttg ccataatttt cctcctcagg ataaagcaaa taaaaaagtt gtgtgtaagc
    18241 gttttcttaa ttataaatac taaagaaatc catttaataa agcaattatt tttttaaata
    18301 taagaaaaaa catttgatag gcaagggtat gataactaaa acttatcgat tttttgaaaa
    18361 gatataagtt cttatgcaaa aatataaaat aatcatctaa atgaagcagt taactatata
    18421 tattagataa gctatggcat aataaataac attttaattt gagtaaagaa ggtatgggta
    18481 tgaagatagg gacaatcgcc gatttgcata ttgatcgtca taataaaaaa acatccgaag
    18541 attacttaga ggcattggtt gaaatagtta agtataagaa attggatata ttgttgattg
    18601 cgggagatat atcaaatcat tatcaattga cgcaccaatt tataacgcaa ttgacaaaac
    18661 aattagatat acctgtgaaa tttgttcctg gaaaccatga tttatgggaa gttgaaagta
    18721 tgactacgca agacatttgg aataattata agagtatgtc acagtgcttg gtaggaaaac
    18781 catttatagt aaatgaagaa tgggcaataa taggacatac aggttggtat gattatagct
    18841 ttgcagcaca acgattttca ttagaacatt tacaaaaagg aaaacattat ggtgcgactt
    18901 ggcaagataa agaacgaata tcttggggca tatcagatca aaatttatct aaaatagcgg
    18961 ctgaacaagt gaagaaagat atattagaag taggaaatag acgagtgatt ttagtcacac
    19021 atgttgtgac gcaccctgat ttcattgttc ctatgccaca tcgtatattc gatttttata
    19081 atgcatttat tgggacaagt gatttcaatc ctttgtatgc gatgttcgat ataccatata
    19141 gtattatggg ccatgttcat tttcgtaaaa gtgtgataga tgatggcaga tgttatctct
    19201 gtccgtgtct aggctatcca agacaatggc gttcagaaga tatttaccag gaaataaatg
    19261 agacgataca aataatagaa atttaaaatg cgcaaacctg acccagtttg cgcattttat
    19321 gttttacaca cgcgagtaat gtgtttactt acgtgtgttt attttgttgt tgattttcaa
    19381 ttgtatatga atgtggttgc acataaatgc actttcttcc tggtgaatta aagctgtatt
    19441 ccattttctc tttacgaatt ttaataattt gtttgcgatt tggaatgatg gcaggtaaaa
    19501 ctaggccacg acgaatatga ctccaaagtg gatcgatata atagtaatag taatcatcgt
    19561 acccaatgag taacgttacg tgaatatttg aaacttgttt cgctggcttg ttatcaaagc
    19621 ggaaaacacg tagtaatggt ttagagccaa gattagtatg gtatattaac acaggttgac
    19681 cttgatcgat aataccttta agatcttcta aggatttacc agtgccgtct acgatattag
    19741 gattgtattt ttgtaaaaat ggtacatatg cttctggaaa tatcgtttga tgataattgc
    19801 caagcttaat gaataagtga tgtccaacat aacctttatg tggattgttc ggatgtgtcg
    19861 gccaatgtct cataatttct gtagcaggga tatgttggtt attgtattgc aacatcatgg
    19921 ctgcggaaac accttcacac cccatgacca tagggatagg aaatagctga ctgataggtt
    19981 taactggtaa tatttttcgg ttcataatat agtcctcgca ttgattcaat aaatatttaa
    20041 tataattata tagcgtcaat gcaaaatgtc ctaaacatat gttttacatg agtgaataaa
    20101 attaatggag tgataaaatg gaatatcaat tacaacaact tgcgtcgtta acgttagtag
    20161 gtattaaaga aacgtatgaa aatggacgac aggctcagca acatatagcc gggttttggc
    20221 aaagatgtta tcaagaggga gtaattgcgg atttacagtt aaaaaataat ggtgatttag
    20281 ccgggatact tggcttatgt atacctgaat tagacggtaa gatgtcatat atgattgcag
    20341 ttaccggaga taatagtgct gatattgaaa aatatgatgt cataacatta gcaagttcaa
    20401 agtatatggt atttgaagca cagggcgcag tacctaaagc agttcaacaa aaaatggaag
    20461 aggtgcatca ctacatacat caatatcaag cagatacggt aaaatcagca ccattttttg
    20521 agttgtatca ggatggtgat actacaagtg aaaaatatat tacagaaatt tggatgcctg
    20581 ttaaagggtg attgaataga aatgtccttg atatgaaggt gtaattttag ttgaagattc
    20641 gatctcataa ttcataaaga agaaccctga caaagataga tatctttgct agggttctaa
    20701 ttatgttgaa gtgttatgcg acatgttgct ttttaatcat tttaaagtaa atgatttgta
    20761 gactgactaa tagaatggct gtaatgaaga taataatcat atatggtgat gtattaaatt
    20821 cgccttttaa gccaactaat ggtgcaacag cgccacctaa gataaattgg aataagccaa
    20881 gtaaacttga ggcgttacca ctgccacctg ttcgttcttc catagccatt gtgaaaccaa
    20941 gcggtccaat tgacgtcaca ggacacacat ttaagaagaa tgcgatgagt aagacccaaa
    21001 gtggtaaatg gaatgtaagt gtcaggataa ttaaagcaac acctaccact tgtataatag
    21061 ttaaaatgat taataatatg tggcgatgta atttttctac taataaagca acgacttgac
    21121 tgacaatgat taaacctaca ccgttaacag caaacatgat actaaattgt tggggtgtca
    21181 tattatacaa tttttgagta ataaatggcg atgcagatga ataactaaat agcattacat
    21241 aagttaaacc ttgtaatagc attggaataa taaacgctgg ttttttcaat agacttccaa
    21301 agtctttaat gacgctacta aaattcacct gctttaattc atctttagat gttttaggta
    21361 attgagaagc gacgccaatt aaaatgatga gtgccacaat agttaaaatt gtgaaaatag
    21421 aacgccatgt tgctacggat aaagctaatc caccagctaa tggtgcaaga atagtgatga
    21481 tgccattaac taccattaaa gatgctaaaa atttagcgag tgcgttgcca ctaaatttat
    21541 caccagcaga ggctttggca atcacgatga cgccaccacc agttaaacct tgaataaatc
    21601 gtaaagttaa gaataatggc aattgatcaa caaacataga caaacctgat accaaaatga
    21661 aaatgatcaa aatggataca gcaatccgtt tgcgaccaaa agcatcggat aaaggtccaa
    21721 agataaattg gcctagtgca agaccaatca ttgtgaatga taatgttaat tgaatttctg
    21781 atgtagtaga accaaaatca ccttgcactt taggtaatga tggtacgtac atatctatag
    21841 ttagtgggcc aaaagcggtc atgacgccta acgtaattag caataaaaat gaaaaatgct
    21901 tcgttttggt tttcgttgat tgcatgatca gactccttta aatgatataa ttatataaat
    21961 tatatacgtt ttgtgtgtat ttgtatgtag ttagaataca agtttatata aaaaatccct
    22021 atactcttaa tgcatcatca cgaataacgt agtgatacag ataaagtata gggaattaat
    22081 gtttatcatc aaagcaggtg taataattaa ttagcgccac cattgtcatc tttaaataag
    22141 ctttctaatt tagatttaat actgtcgaat tttgtattaa tatcatttaa catgctttca
    22201 attttacctt ggcttgcatt gtttttaagt gcattatttg cctctgtcaa tttgttgatt
    22261 tggtcttgat aaaattgagt tagcttagaa tcagctttgt tttgttcttg ctgcttcaag
    22321 ttgtcgattt gattttgtaa atttttaata atgtcatcag ttttttggtt gtttttaata
    22381 ttttctttag cattatctac ttgctggtta atttggccgt cttgattttc aacttgtttt
    22441 ttataatttt gagagacttt agggtcatta ctaactttat cgctatcatt gttaagtaag
    22501 gcgcgtgtga caaaaatagc gacaataatt aagagaatgg caataatgat atataaccat
    22561 ttttttgaac ggcgggattt ctcgttttct tcttcaagaa tttgttctct tcgataatct
    22621 ctaccattat agtatcgttc cgggtgttct tcaggatcgc ctttagcata ttgttgtcta
    22681 tacgatgcgt cttcagattg acgacgatga ttttggtttt gattgtcgta ttgatatgaa
    22741 tcacggtatt tatcagttct tttcatgggt ttcacctcat tcaagtttta actgcaaata
    22801 ataaagctga tgatttactt tgacattatt gtaatttcag aatacgagca atgtcagttg
    22861 cagtgtttaa taaattttgt tcgctatttt gtaggacatc ttctaaagtg ctaggtgaag
    22921 agattataga ataggcacta tcaataccga aatcgtaaac atgttgataa ttttcgccta
    22981 gactgccaca aatcgcgatg acaggaatat gatattgttt tgcagctaac gcaacgccta
    23041 cgggtgtttt accaaagatg gtctgataat ccatgcgtcc ttctccagta ataacgaggt
    23101 ctgcatcttt aattctttga tgaaaatctg taatgtcaaa gacgacatca ataccttttg
    23161 ttaaagttgt ctcacaaaac gctaataatg ctgcgcccat accgccagct gcaccagaac
    23221 ctggtatttg attaacggac tttcctgtgc acatttttat cttatcatga taatgcgaca
    23281 ttgcgaaatc caactttggt atcatctttg catcagcgcc tttttgagga ccataaatat
    23341 aggtagcacc attttcaccc aataaaggat ttgaaacatc acaggccact ttaaaggtca
    23401 cctcttttaa tcgcgaatct agattggtta tatcgatttg tgcaatgtga gcaagattag
    23461 caccattcat ttgtaataag tccccgttta catcagtaaa ctttacgcct agtgcactta
    23521 gcatacctgt accaccatca tttgttgcac tgccaccaat ccctaaaata atggtcttag
    23581 caccatgatt taatgcatct ttaattagtt caccggtacc atatgatgat gtgtataaag
    23641 gattacgttc ctctttttct aataaatcca aacctgacgc tgccgccatt tcaataattg
    23701 caatttgttg ttcgtctgcg cgtgcataac atgcttcaat aggtcgcatt aaagggtcat
    23761 taatgatgac tgtatactta gtggcacctg ttgcatgcat taaagcatct gtggtacctt
    23821 caccaccatc agccatcgga atgatatcat aatgaagggt attcccataa acattagtaa
    23881 atgcctgttt tataatattg ccaacttgct gtgcggtcat actttcctta aatgagtcag
    23941 gagcgatgac aattttttgt aattgcatag tagacatcct ctcattactg ttgtcgctct
    24001 tattgtatat aaaattacta gtgaactaaa gtaatctcta tagtgctaat gagtcacgcg
    24061 ataaatcagt tacattataa tggcgtataa ggatgagagg aaagagacta aatttgctgt
    24121 gaaagggtat aaagattgaa ataagcatat ttgaagtgaa gcggattggc acgcaatttc
    24181 gagaacgtag ttatgcatat attttgatgt tgaggagtta cacatatgaa actaacacaa
    24241 acgcatgctg aaattttaaa atttatcatt gttggcggta ttaatacgtt gaattattat
    24301 gttgtctatt tattgctgtt aaaattattt cacattgaat atatgattag tcatattacc
    24361 ggatttatag ttgcttttgt gatttcttat tatttgaatt gttattttgt ttacagagta
    24421 aaacctactt ggagaaaatt cattagtttt ccaattacgc agattgtcaa cgtaagctta
    24481 caaacagttt tattatatgt ttttgtatcc tggctaaatt tgccagcgga atttgcgcca
    24541 tttgcaggtt tagtgattac gatacccatc acatttgtat tatctaaatg gattttaaaa
    24601 gatagtaatt aaagtagcgt aaaccactaa aaaacgcttt actcgaactt taatattgcg
    24661 tgaaatataa taaataaatg atataatata atggtcgcct aacatctcgg gcgttacttt
    24721 aacgcagaga ggaggtgtat gacggatgtt caatttatta attgacatca tgacttcagc
    24781 tttaagcggc tgtcttgttg cgttttttgc acattggtta cgaacgcgca acaataaaaa
    24841 aggtgacaaa taaccaacac ctaaataaat cccctcacta tggcagtagt gaggggat
//
LOCUS       DAHHYJ010000023        24714 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000023 DAHHYJ010000000
VERSION     DAHHYJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.023"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(394..1734)
                     /gene="glnA"
                     /locus_tag="NBK22_000717"
     CDS             complement(394..1734)
                     /gene="glnA"
                     /locus_tag="NBK22_000717"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCD2413920.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            complement(1753..2121)
                     /locus_tag="NBK22_000718"
     CDS             complement(1753..2121)
                     /locus_tag="NBK22_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD2413921.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            complement(2364..3602)
                     /locus_tag="NBK22_000719"
     CDS             complement(2364..3602)
                     /locus_tag="NBK22_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD2413922.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            complement(3621..4859)
                     /gene="hflX"
                     /locus_tag="NBK22_000720"
     CDS             complement(3621..4859)
                     /gene="hflX"
                     /locus_tag="NBK22_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCD2413923.1"
                     /translation="MAQQQIHDTKNKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRQLTYYDETIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            4971..5447
                     /locus_tag="NBK22_000721"
     CDS             4971..5447
                     /locus_tag="NBK22_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD2413924.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRIAPQKKPVQIEREIEKLL"
     gene            complement(5669..5902)
                     /gene="hfq"
                     /locus_tag="NBK22_000722"
     CDS             complement(5669..5902)
                     /gene="hfq"
                     /locus_tag="NBK22_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCD2413925.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(5917..6852)
                     /gene="miaA"
                     /locus_tag="NBK22_000723"
     CDS             complement(5917..6852)
                     /gene="miaA"
                     /locus_tag="NBK22_000723"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCD2413926.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLAQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELIPVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            complement(6870..7784)
                     /locus_tag="NBK22_000724"
     CDS             complement(6870..7784)
                     /locus_tag="NBK22_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2413927.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            complement(7934..9607)
                     /locus_tag="NBK22_000725"
     CDS             complement(7934..9607)
                     /locus_tag="NBK22_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCD2413928.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDAVIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            complement(9765..11261)
                     /gene="glpK"
                     /locus_tag="NBK22_000726"
     CDS             complement(9765..11261)
                     /gene="glpK"
                     /locus_tag="NBK22_000726"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCD2413929.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            complement(11391..12209)
                     /locus_tag="NBK22_000727"
     CDS             complement(11391..12209)
                     /locus_tag="NBK22_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCD2413930.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            complement(12684..13226)
                     /locus_tag="NBK22_000728"
     CDS             complement(12684..13226)
                     /locus_tag="NBK22_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCD2413931.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKIDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            complement(13232..15241)
                     /gene="mutL"
                     /locus_tag="NBK22_000729"
     CDS             complement(13232..15241)
                     /gene="mutL"
                     /locus_tag="NBK22_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCD2413932.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMVENQNDEIVIR
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDDTSVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            complement(15254..17872)
                     /gene="mutS"
                     /locus_tag="NBK22_000730"
     CDS             complement(15254..17872)
                     /gene="mutS"
                     /locus_tag="NBK22_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCD2413933.1"
                     /translation="MSNVTPMMKQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNSRDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            complement(18175..18666)
                     /gene="thiW"
                     /locus_tag="NBK22_000731"
     CDS             complement(18175..18666)
                     /gene="thiW"
                     /locus_tag="NBK22_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCD2413934.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            complement(18693..19058)
                     /locus_tag="NBK22_000732"
     CDS             complement(18693..19058)
                     /locus_tag="NBK22_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCD2413935.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            complement(19059..20603)
                     /gene="miaB"
                     /locus_tag="NBK22_000733"
     CDS             complement(19059..20603)
                     /gene="miaB"
                     /locus_tag="NBK22_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCD2413936.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            complement(20737..21030)
                     /locus_tag="NBK22_000734"
     CDS             complement(20737..21030)
                     /locus_tag="NBK22_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCD2413937.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGMHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            complement(21124..21990)
                     /locus_tag="NBK22_000735"
     CDS             complement(21124..21990)
                     /locus_tag="NBK22_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCD2413938.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATRTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            complement(21991..23751)
                     /locus_tag="NBK22_000736"
     CDS             complement(21991..23751)
                     /locus_tag="NBK22_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCD2413939.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIDGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGVKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIESPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLASIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            complement(23891..24688)
                     /locus_tag="NBK22_000737"
     CDS             complement(23891..24688)
                     /locus_tag="NBK22_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCD2413940.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
BASE COUNT         7481 a         4725 c         3386 g         9122 t
ORIGIN      
        1 aatgcgtagt aaggtccctc ttttcggggt cttaccttag aacgttctgc aaatgaattc
       61 gatgagaagt aatatgaata tggctatttt caagtaatac tcaacgtttt cgcgacgttc
      121 ttttatcgcc tcatctcatc acctccaaat atattaaaat tcatgtgaac taaaatataa
      181 aatggtcttc cccagcttta aaaaaataaa tacataaaac atttacttga ccaaaacttg
      241 accaaaactt gaccaaaact tgaccaaaac ttgaccaaat ccatttttta acaaaagtgg
      301 tcaagtgaaa tcccatcttt ccaaatcaaa aaagagctaa agcaaaacgc tctagccctt
      361 gatattactg atttcccatc attgtttagc gtattaatat tgcttcatgt actgatctct
      421 ttcccattca gatacttgag ttctgtagta atcccattca attgattttg aattaataaa
      481 ttgattatag atatgatttc ctaaagcttt tttaataact tcattttcac gcattgcttt
      541 taatgcagta taaagtgttg aaggtaagtc ttgaatgcct actgcttcac gttcttcacg
      601 gttcatttcg taaatatttt ggttaactgg ttctggaact tttaatttat ttttaatacc
      661 atctagtcca gcttctaaga ttgcagctaa cgccatgtat gggtttgcag ctggatctac
      721 tgaacgtact tcgatacgag tagataatcc tcttgaagat ggtacacgga ttaatggtga
      781 acggttttta ccactccatg caatataaca tggtgcttca taaccaggta ctaaacgttt
      841 atatgagttt actaacgggt tacatacagc agtaaatccg cgtgcatttt taagcacacc
      901 tgctgtaaat tgatatgcag tttccgttaa gcccatttca gtatttggat caaagaatgc
      961 attttcttta cctttgaata atgaaacgtt aaagtgcata ccgctaccat tcacaccgaa
     1021 taatggttta ggcataaatg ttgcgtgtaa attatgttta cgtgcgattg ttttaacaac
     1081 caatttaaat gtttggatat tatcacatgc tgtaacagca tctgcatatt taaagtcaat
     1141 ttcatgttga ccaggggcaa cttcatggtg actagcttca atatcgaagc ccatatcctc
     1201 taattctaaa acaatatcac gacgacagtt ttcacctaaa tctgtaggtg ctaaatcgaa
     1261 atatccacca tcatcattaa gttctaaagt tggttcccct ttttcatcca acttaaacaa
     1321 gaagaattct ggttcaggcc ctaggttaaa gtctgtgaag cctaaatctt ccatttcttt
     1381 taatacacgt tttaagtttg cacgaggatc cccttcaaat ggtgttccat ctgttttata
     1441 tacatcacaa attaaacgtg caactttacc ttgtccagca gtccatggga agattaccca
     1501 agtatctaaa tctggatgta agtacatatc tgattcttcg atacgtacga aaccttcgat
     1561 agaagaaccg tcaaacatca tttcgttatc aagtactttt tctaattggc ttacaggcac
     1621 ttcaacattt ttaattgttc ctaaaatatc agtgaattgt aatcttaaat atcttacatt
     1681 ttcctcttct gcaaatttac gaatgtcgtc tttagtgaaa gtacgttttg gcattttaaa
     1741 atcctccaaa ttttatttaa taaatcggga taaatcacca cgatttattg gcaaagtttc
     1801 tccaataggc ttttgcgtgg catctacaat catctttttt cttatctctt gttcatctgt
     1861 tgttaaatgc tcttgtgagt cataaatgat ttgtttaatc cctttgatat taaatccttt
     1921 ttctaataat gatttaattt ctagtaatct ttccaaatca ttgagtgaga acagacgttt
     1981 ttgaccttct gttctttcag gtttgatgag ttcatgtgtt tcatagtaac gtatttgcct
     2041 tggcgttaaa tccgttaact tacttactac actcatagag aagacagcca tatttcgtct
     2101 gattgcatca ttcgatatca ttgttcctct cctctacttt tgaacttgtt aataacttat
     2161 cataattttt ttaggatttc aaaaatatgt caggttttct aacataaagc ttaaaacatt
     2221 gatataactg cgatttcgac ttctaaaacg acataaaaca atctacagct aaaatatttt
     2281 ttcaattttt aaaaataatc tgataaatct ccgcaaaaaa tttcacccaa aacacattat
     2341 catttagggt gaaatttaat ctattatata agtccttgtt ctttcaactg attaacagct
     2401 cttgtcacag caattttaac gtgttcatat gttaatcctc cttgaacata tgcttcataa
     2461 ggaggacgaa taggtccatc tgcagataat tcaatcgatg aaccttgaat aaacgtacca
     2521 gctgccataa taacatcatc ttcgtaacca ggcatataac taggttctgg actaaaatgt
     2581 gcattaattg gggatgcgtg ttgaatactt tgacaaaatg aaatcatttg ttctttcgtt
     2641 tcaaatttaa ctgtttgaat taaatcagtt cgtttttcgt agtacttcgg cgttgtgttc
     2701 atattcattt tttctaaaaa taaactagta aacaatgcac ctttaagact ctgactgaca
     2761 acgtgtggtg ctaaaaagaa accttgatac atttcaagca atgcatttaa tgatgcaccc
     2821 gcttctttac caataccagg tgctgtcaat ctataaccac atcgttcaat taaatctttt
     2881 ctaccagcaa tgtatccacc aatcttagct aaaccaccgc cagggttttt aattaatgat
     2941 cctgctatta aatcggcacc acattctata ggttcacgtc tttcaacaaa ttccccataa
     3001 cagttatcca caaatattaa aatattagga tgcacgtttt tcaacctagt aattaccttt
     3061 tcaatttcat ctagcggaat tgaaggtctt tgatcatagc ctttcgaacg ttgaatcgct
     3121 attactttgg tgcgctcaga aaccccatct aacacacttt caatatcgat cttaccttct
     3181 ttaagtgcaa tatctttata cgatacgccg tgctccatta aactttcaat accatttccg
     3241 tttacgccaa tgacttcaag taaagtgtca tatggactac ccgttatata aattaattca
     3301 tcaccatgtt ttaataaact ttgtaatgca atagtaatcg catgcgtacc tgaaataatt
     3361 tgcggacgaa caattgcatc ttctgcttta aatgcctgcg catatatttc ttctaaatga
     3421 tcacgtccaa agtcatcata cccgtatcct gttgatcctt gtagatcact ttcggttgct
     3481 ttgacatgat gaaatgcatt taatactttt tcttgattaa tatatgctgt ttcttcaatt
     3541 tctttaaaat atggtgctaa cgttgattcg acgtcagcta ctatcttact tatatctttc
     3601 atttgaattt cgtcctttta ttatttttta aatcctttta tacgataaac attttcaatt
     3661 tcatcatatt taagttcagt cactaatgta tgttgtttta gaaaatataa tcgatctgca
     3721 ttattcgtcg caattgtctc atcataataa gttaattgcc ttttgatttc atcaataaat
     3781 aacgtcttaa caagtaattt atcaccatca tttttagaag aaacaaaaac attaggcaaa
     3841 tcacttgctg gacgatttga tgcatgatca cataagtcct ttttattaaa aataactatt
     3901 tgagaaatat gactcatatc taattgtttg attaaatcat taactgtgtc atactgcgta
     3961 cggtattcag gatggctact atctacgaca tgtactaata aatctgcacc tttagcctct
     4021 tctaaagttg atttaaaagc tgcaattaac gtcgtaggta gtttctgtat aaaaccaaca
     4081 gtatctgaaa taattaaatt aaatccatca tttatttgaa tttgtcgtgt tttaggatct
     4141 aacgttgcaa ataattgatc tttttcatac gtctcttcat ttgctaaaac attaaaccat
     4201 gatgatttac cagcatttgt ataaccaact aaagctactt gaaacacctg attttgattt
     4261 cttttatttc gatatctttc gcgatgttct tctaccgtcc gcaattgatg tttaatttca
     4321 ttcatacgag ttcgaatatg tctgcgatcc atctctaact tcgtttcacc agggcctcta
     4381 gttccaatac cgccacctaa acgagaaagg cttttaccat ggccttgcaa tctaggtaat
     4441 aaataatcaa gttgtgctag ctctacttgc aatttacctt ctttacttct tgctcttaat
     4501 gcaaatattt caagaatcaa ctgagttcta tcaataattt ttacacctaa agcttcattt
     4561 agtgatttgg attgtgcagt cgttaattca tcatttgtga tgactacatc aatatctttg
     4621 aactcaataa atgcttgaat ttcttcaatt ttacctttac caacataata tttgcgatct
     4681 acacgatctc tgttttgagt aatttgaccc aacacttcaa gttggcaagt ctctgataaa
     4741 gaagataatt cctccattgt agactcaaaa ttaaattgct tatcatcttg agcatgtaca
     4801 ccgactaaga cagctttttc tagtttattt ttagtatcat gaatttgttg ctgagccatg
     4861 ttatacatct ccttaataaa atcctactca aagtattcta tcatagagtt ttagtaaaga
     4921 ctattacacg attttgtggt acattttaag taaatcgaaa ggatgataca atggagacaa
     4981 tttatgattt tgtagtagaa acaaataaag gtgttactta caaattagat gcatataagg
     5041 gtgacgttat gctaattgtt aatacagcta gcgaatgtgg ctttacttca caatttgaag
     5101 gtttgcaatc attatacgag aaatacaaag atcaagggtt tgtaattcta ggtttccctt
     5161 gtaatcaatt tggtggccaa gaacctggtt caggcgaaga agcagctcaa aactgtaaat
     5221 taaactatgg tgttaccttc cctatgcatc aaaaaataga cgtaaaaggg gaacatcaat
     5281 tacctttatt tagatatttg actgcggcac aacacggatt ctttaatgag aaaatcaaat
     5341 ggaatttcac taagttttta gtggatcgcg aaggtaacgt tgttaaacga attgcacctc
     5401 aaaaaaaacc tgttcaaatc gaaagagaaa tagaaaaatt attataaaat atactagctc
     5461 atagcaaata aaacgcttca tataaagtcg tatcatatat tgactcgacc tttatatgaa
     5521 gcgtttttat tacaaacatt cctctattgt atcttgtcat ttgaacatca tttaattgat
     5581 gtcacatatg actttatttc tatatctatt tatcatactt caaacgttat gtacacgagt
     5641 cattaaatcg tgcccatatg attacaactt attcttcact ttcagtagat gcttgacctt
     5701 cagtttctac tgtataagtg ctgatcgcat gtttgtaaat caagtgttgt ttgccttgag
     5761 aatttaagct tacgacatac ttgtcgtatt cttcaataac acctttcatt tggaaaccgt
     5821 ttagaaagaa tacagttact tcagtttggt ttgctttaaa attctctagt gctttgtctt
     5881 ggatgttttc gtttgcaatc atctgtcgga ctccttttac ttaatctggg ttgtaatctc
     5941 atctaacatc atttgaagtg acatattttc tttatctaac caatgaacac tcattttatt
     6001 cttgaaccat gtcatttgtc gttttgcata ttggcgtgaa tgttgcttta aatcattgac
     6061 agcatcttca taaatcattt gtccgttaat cacaggtatt aattctttat atccaatagc
     6121 ttgcatactt tggcaagatt catagccttg ttcaacaagt tgttgcactt ctctaaataa
     6181 tccgtgatcc aacataatat caacacgttt atttattctt gaatataatg ttttacgcga
     6241 catttcaatc cctaataata atgtatcata attttcagta aattgttgca ctttcttgcg
     6301 attactcaaa agtttttttg tttttaaata atattcaata gcgcgcaaca ctctttggcg
     6361 gttgttaggg tgaatatttt ctgcagaaac cgcatcaaat tgagctaaat aatcgtgtag
     6421 ttgctgatta tctaaatgtt ctaatgcaga taacttttgt ttaactatgg ataattgtgc
     6481 aggtgtaact gtttcatctt ctaattcata attatatatt aatgattgaa tatataagcc
     6541 tgttccacct gctatgattg gaactttacc tctattcgtt atatcagtaa ttaaatcttc
     6601 tgctaatcgc ttgaattcat atgctgaaaa tgtatcatca ggattcaaga tatcaattaa
     6661 atgatgtgga ataccatcca tttcttcagg tgttactttt gcagttccaa tattcatatg
     6721 tttgtaaact tgcatagaat caccgcttat gatttcacca ttgatacgct tcgcgagttc
     6781 tatgctaagc tctgttttac ctgaagcagt tggccccaca attactacaa taaaaggctt
     6841 atttttattc atatttaatt ccactttctt tattttttat aaaattgatt caaccattta
     6901 aacaagtctt cccaaatttt gtcatgatct ttttcaaata acacttcatg acgtttatga
     6961 tgataaagat tcactttaac atttttaatg ccagcttttc tatatatatt cgccaatttt
     7021 aaaatccctt taccataatc acctaaagga tcgtcatatc ctgaaactaa taatataggc
     7081 atggcatgat ttaacatttt catatttttt aattgtgatg tatgtagcat ataataggct
     7141 gtttgatata ataattgatt tgacacatta aatccactat atggatcttt aataaagtta
     7201 tctacttcaa ttggattact agaaatccaa tcactttgtg tacgtaatgg acgtattttt
     7261 ttattaaaac tatttgatac taactggtta acccattcaa ctcgtttctc agcaccataa
     7321 atttttgtaa tcagttgtaa cactttaacg gttggtaaac ctttccataa tgaatacata
     7381 ccagtaccac ttaaaattag accatcaaca tattgcggat atgtttctac aaacaatcta
     7441 gctataacgg atcccattga atgtccgatt ataatgtatg gtttatcaac attgcctctc
     7501 actgtttgcg ctacttcaaa ggcatcaccg ataactcgtt tcatatcatc gtaatgccct
     7561 cttgttgatt catcaatatt aataccatgt cctcgatgat tatgacgtat cacatcgaag
     7621 ccatgctttg aaagtgcatg tgctaattta tcgtaacgtt ccatatgttc agccatacca
     7681 tgaaatatat gaataattcc ttttgaatct acatctgtaa aattcacttt aacttcaata
     7741 ttcgtattat cttgtacagt taatttaaat tgtttttcag tcatcatctc acacctttaa
     7801 taacaacgct tttatattcg cttttattat tatcatacat ttagtgatgc atagtttcaa
     7861 ttgactaatg tatctcgtat aaaaaaactg atgcccttaa aacaggacac cagtttatga
     7921 ttgtacaaat taattattct ttaactgctg gttgattatt tccatgttgc gcttctttaa
     7981 ttgcttgctc aacttcttca gtatatgctt caatttgagc tggagagtaa tcaagcatat
     8041 ctgccataat atcgatgaca gcatctttat aatctaatac atctttaata ttgaaataca
     8101 ttttaccaga acgacgaact aagaaatcgt taggtttgta taccatttct tgttgaatac
     8161 tataaacaag ttctacataa atttctaatg gtaacttgct atcatggtat tgagatgttt
     8221 gcgcaatgtt gaacaattca tcaacatttg aaccatattt agatgctaaa cgtcttgcaa
     8281 catcttcatc aatgccgaat cctttagcta catctacttt ttgctctaca aacgcatcaa
     8341 agttcttgct accacctaca tcgccacctg aaattgccag accttttgta ttacatggac
     8401 taaatgttaa accgtagtct tttttcaagc gtttagatac taaatcaaca atgtcttgag
     8461 ccatgtgacg atagcctgtt aatttaccac ctgcaatagt taataaacct gatttacctt
     8521 cccaaatttc atccttacga gagatttcag aagggtcttt gccttcttcg taaattaatg
     8581 gtctaattcc tgcccatgtt gattcaatat cttcatctgt aacattaaca ctagggaaca
     8641 tgtaattaat cgcatcgatt aaatagtctc tgtcttcttg tgtagttaat ggtgaagatt
     8701 tgatattgtc atagaatgta tctgtagtac ctacatacgc ttttccttca cgtggaattg
     8761 caaaaatcat tcttccatct ttttcagtat caaagtatac tgcttgacct aatgggaatt
     8821 ttgattgatc aataacaaca tgtacacctt tagttaaacg taatttttta ttattgcgtg
     8881 cataatcacc acttctaaca tcatcaaccc atggacctgc tgcattaacc acttttttag
     8941 ccttaattgt ataattttca ttagttaatt tatctataac tttaacacca tttacttgtt
     9001 gatttttatc ataagtgaag tgttcagatt tagtatagtt gataatttct gcgccttttt
     9061 cagcagcacg cttcataact tcaatagtta aacgcgcatc gtcagtacga tattcaacat
     9121 agtaaccgcc gccttttaga ccttcttttt taactaatgg ttctttagct aaagtttctt
     9181 ttttagataa cattttttta cgttcagact tcttaacacc tgctaaacga tcatacattc
     9241 ctaaaccaat tgatgttgag aatttaccaa atgttccacc tttatgcatt ggtaaaagca
     9301 tccactctgg agtcgtaaca tgaggcccat tttcataaac aatcgcacgt tctttaccag
     9361 tttcggcaac tactccaatt tggaattgtt ttaagtaacg caaaccacca tggactaatt
     9421 ttgtagatct tgagcttgtt ccttgtgcaa agtcttgcat ttcaactaat gcaactttca
     9481 ttcctctttc actcgcgtct agtgcaatac ctgcacctgt aataccgcca ccaataatta
     9541 ctaaatcata ttcatcattt cttaaattct ttttaatatg ttctctctta aaagtagaca
     9601 atgccattcc aaaacgcctc ctaaaaaatg taaaaacgag agacttgtcc cacataaaca
     9661 atccttcaat tgtacatgtt cgaacaaatc tctcgcttct cgattcaaat attaacttaa
     9721 attgattgta tcacaatgtt tatacaaaag tctaatctac aagtttattc tgttttaaaa
     9781 acttgtgttg cttcaacagc ttttttccaa cctctatata atttttctct ttcgccttca
     9841 tccattttcg gatcgaattt ttcttctaat ttccagtttt tagcgatatc atctttactc
     9901 tcccagaatc caactgctaa acctgctaaa aatgcagcac ctaaagctgt agtttcttga
     9961 atttcaggtc tttcaacaga agtattaaca atgtctgctt ggaactgcat aataaagtta
    10021 tttttaactg caccaccatc gacacgtaaa ctttggacat caataccaga gtcttttgac
    10081 attgcttcca taacgtcacg agtttggtaa catagtgatt ctaaagttgc acggataaag
    10141 tgctcttttt cagttccacg tgttaaaccg aaaatcgcac cacgtgcttc agaatcccaa
    10201 tatggtgttc ctaaacctac aaaagctgga acaacataaa caccctcagt agagtcaact
    10261 cgtgtcgcat aactttctga ttgtggtgct gaattaatca ttcttaatcc atcacgtaac
    10321 cattggattg ctgaacccga aacaaagatg gaaccttcaa gcgcataatt tacttttcca
    10381 tcaataccat aagcaattgt tgttaataaa ccactttcag atttaaccgc tttgtcacct
    10441 gtattcatta acatgaagcc accagttcca tatgtgtttt tcacgtcacc acgttcgaag
    10501 caagcttgtc caaataatgc tgcttgttga tcaccagcta ctccagcgat tggtacttct
    10561 tgaccataga agtggtaatc aattgtctta ccatatactt cactcgaagc tttaacttct
    10621 ggcaacatat ttttaggtac tgtaagtagt tctaataact catcgtccca ttctaaatca
    10681 tggatattaa acattaatgt acgactcgca tttgaataat cagtaatatg cgcagctttt
    10741 cctgataatt tccatactaa ccaagtatca atcgttccaa ataatagatc gccattttct
    10801 gctttttctc gtgcaccttc aacattgtct agaatccatt taacttttgt acctgcaaaa
    10861 tacggatcta aaagtaatcc tgtcttatct ctaaatgttt gttcatatcc ttgttgtttt
    10921 aactctgaac aaattgattg tgtttgacgt gattgccaaa caattgcgtg ataaattggg
    10981 cggccagtat gtttgtccca aacaaccgtt gtttcacgtt ggtttgtaat accgattcct
    11041 gcaatttgat cagctctaac atcattttca ttaattactt ccgtcattac agctaacaca
    11101 gatgtccaaa tttcatttgc atcatgttca acccaacctg attgtggaaa atattgctta
    11161 aactcacgtt gtgctacccc tgcaatttcc cctttttgat tgaataaaat cgctcttgag
    11221 cttgttgttc cttggtctat agataaaata tatttttcca ttagtagtat ctcctttatt
    11281 aaaaaaataa tcgctaaccc attgtacaaa atctgttaaa ttttggcgaa aaaatagcat
    11341 aatccatgaa tcaaacatgt taaattatgc tatttacgtt tctttttatt ttagtaaatt
    11401 gattcgatat cacctttttt tgatgattta tttaaaatgt aacctaaaat caaagtaata
    11461 ataactacaa caattgcaat tgcacaacca atattaaatg tatgtttata aaatacagcg
    11521 tataccactg cacctaataa accaccggca attggtccta agataggaac gattgcatat
    11581 gaccaatttg aaccaccttt accagctatt ggtaaaatcg catgtgcaat tctcggacct
    11641 aaatcacgtg ctgggttgat tgcataacca gtagcaccgc ctaaacttaa tccgattgca
    11701 acaattaatg ctccgacaat taaaggattt aaaccatcgg caattttgtt tacaccgata
    11761 aataaaatac ctaaagttaa tgccattgtt ccgataatct cacttaaaaa gttggcaaag
    11821 taattcttaa tagccggtgc tgtagagaaa acacctaatt tcgcgccagc ttcttctgtc
    11881 gctttccaat gtggcaagta cattaaccat acaattgttg ctccgacaat tgcacctaac
    11941 atttgagcaa caatataacc aggaactaat gaccaatcaa aacttccgtc taatgcaaga
    12001 gctaaagaca ccgctgggtt taaatgtgca cctgagaatt gaccgacagc aaacacaccc
    12061 attgtaaccg ctaatcccca tccagctgtg atgacaatcc aatcagcacc attcgcagca
    12121 cttctcttta aattgacatt ggcacaaacg ccaccaccaa aaaggattaa gattgcagtt
    12181 cctaggaatt ctgctaaata tacattcata ttaaatgtcc tcccaaaaag acaaagagat
    12241 tcctacacta gcagtttggc tcgtagaaat ctcccgtctc tattctatgt attaactttt
    12301 attatatttg ttatgatact atcgatatga aagcgttgtc aatggttttt gtaaaaattt
    12361 tgtcaaattt aatttttcaa gtctttataa aagtagttta atttgaaaac tagaaatatc
    12421 aatccgcact aatttattta tgacgccatt atgtttaatt gacattcttc agccatattt
    12481 tttatgacta gttgcatttt ttactaataa acacctctaa aactttaatg attttaatcg
    12541 ttttagaggt gctaattatg ttatttaatt attctttgta tgatacctta aataccttat
    12601 tacatatccc atatgaaaac ggctttttta atatgttata tgactaaatc tcgtgaaaaa
    12661 tgaaattttt gcagacttat gatttaccaa agtttatcat aactagttgt tacatatttc
    12721 gctccatttt taacagcttc attgacttca tctattgtat taattaggcc acctgcaatg
    12781 acttgtgtgt ttgtttcttt ctgaatatga tgaatcgctt tactcgcaac acctggaagt
    12841 acttcaacaa aatcaggttc aacttttttt atcaaatcta tactgcgttt caatgcttga
    12901 ctatcaataa taaatactct aaaaatcgtt aaagtattta atgatttagc tttttttatt
    12961 actttagatt tagtcgatac gatacctttt ggcttgtatt gctgaataat aaattcactt
    13021 gcaaattcat cgtggcttaa accttttatc aaatctatat gaataaaaca ctctatatga
    13081 ttttgcttca gcaattccat aatacttttt atatgtccta tatgcatatc tagaagcaca
    13141 cacattttat aatctatttt aatcagtttc tctaaatctt taatgtttct tatggcaggc
    13201 aatatgttgt tattcacttg attcatcctc tctacatcac acgcttaaat aatttttcta
    13261 attcgtattt tgaaaaatta atgataattg gacgaccatg tggacatgta aatggatctt
    13321 ccgcttctct taattgatca attaaatcag acatttcatg tttttgtaaa taatgattcg
    13381 ctttaataga ttttttacat gacatcatga ttgctacatc ttcacgtaat tttttgatat
    13441 ctactttttt ctcttccaaa attagctcaa tcatatcttt aataatttct tctacttcat
    13501 ctttagggaa ccaaactgga tagctactta caatataatc atgaccacca aaatgttcta
    13561 acatgatacc tacttgttga agctcatttt tatattgatc aatgactaat tgttcatctt
    13621 ttgaaaaatg aaatgttaac gggattaata aatcttgtac ttcattggta acttcaccta
    13681 ttttatctcg aaaatattca tattttattc tttcttgagc tgcatgctgg tcaatcatgt
    13741 acatgccaaa ttcattttga gcaataatat acgttccatg tacttggcca acaatttcca
    13801 tatatggaat tcttcgttta gggtctttac tttttgttcc tttaatatcg tttacatcat
    13861 aataatcatc agacgcttta ttctcataat tttgcacaga tgtatcgtca ttcgaatcaa
    13921 atgtttgatc catttcttgt agaatttctt gttgcttttt gaagtagtct tcatcttttt
    13981 cacgtgtatt attataaccg gaagattcat catcagctat taaactttca gacgttttcg
    14041 ttacgaatgg attatatgaa tcttctctta taactatttc atcgttttga ttttctacca
    14101 taaatggctt actgttactt tcttcagatg aaaacgtctt ctcttgatta ttctctgtgt
    14161 tttgtctttg ttcaaattcg attttttgtt gttcgaatga atgtaacact ttattttttt
    14221 tcggcacata atccaagtta tttttaggaa ttaatatacg gtctttaaat gcttcttgta
    14281 ttttgctcac aatcaattgg tatagttgct cttcttttga taaacgcact tctagttttg
    14341 ttggatgaac atttacgtct actaagattg gatccatttc aatattaata taacaaatcg
    14401 ggaacctacc tattgttaag agtgtatgat agccttctaa aatcgcttta tttagcataa
    14461 agtttttaat gtatcgtcca ttaataaaaa tagaaatata gtgcttatta cttctagaat
    14521 gttcaggctt tgcaacaaaa ccttcaatgt gataatcact tgtatctcca gatatatgta
    14581 ctaaatctcg tgcaactttc atcccataaa tctctgccat cacttcatta gttcgtcctg
    14641 aaccatttgt acttaacatt gttttgccat ctgaaatgag tgctattcga atgtccggat
    14701 ggctcattgc cattctgttg acaatatctg ttattttacc tagttcagtg tataaacttt
    14761 taatatattt taaacgtgct ggtgtattat aaaataatga ttctacaagt atatctgttc
    14821 cttttttcgc ttttgcaggc ttatgattta atatttcacc attttctaca tatatttcat
    14881 ttccattagc attatccgtg caagtcttca atgttacttt agcaactgat gaaatactgg
    14941 ctagtgcttc accacggaat cctaatgtcc taatatgaaa taaatcttca tcttgatcta
    15001 atttactagt cgcatgtcta tgaaatacta atcctaagtc ttccgcttca attccgcttc
    15061 cattatcgac tacgcgaata gattggacgc cagattcctc tacttcaatg cttatttctg
    15121 tagcgcctgc atctatagca ttttccaaca gttctttcac aacagaactc ggtctttcaa
    15181 ctacttcacc tgctgcgatt ttatttgcta atgaggtttg gagttcttta attttcccca
    15241 ttttgcaaca cctctatttt aattgatttt gtaattcact taacttcacc aatgcctcaa
    15301 ttggtgtcat attagataaa ttcaaatttt taatttgtag ttcaatctcg ctttcttgat
    15361 cattttcaaa caaatcaaat gatgcttgtt caaagtcttt ttgagataaa gtatcagttg
    15421 tttcttcaac acttaagttt aaattttctt gattaatttc aggttcattt tcgaccattt
    15481 ttaaatttga tatcgatgat tttttaccag cagacgcttc aaactcgctt aggatcactt
    15541 gtgctctgct aataactttt tcaggtaaat cagctaattt cgcaacttga ataccataac
    15601 tatcgtcaac tgcaccatct ttgactttat gcaagaatat aagttcacct ttatattcat
    15661 tagcagcgac gtgaacattt tttagacttg gtaatgcttg atctaatgtt gtcaattcat
    15721 gataatgtgt tgaaaataac gttttagcat gcgatgtttc agctacatac tctatcattg
    15781 cctgcgctaa agctaaaccg tcatacgttg aagtaccacg tccaatttca tcgaaaataa
    15841 tcaaactatc ctctgttgca taagttaatg ccttttgtgc ttctagcatt tctaccataa
    15901 acgtactctt acctgaaacc aaatcatctg ccgcacctat tctagtgaat atttgatcaa
    15961 atataggtaa cactgcctct ttacaaggga cataagctcc catttgggcc attatactaa
    16021 ttatggcaac ttgtctcata tatgtcgatt taccagacat attcggacct gtaattaaat
    16081 atataaatgt ttcattatct aatcgacaat tattaggcac atagtcatta taatccatta
    16141 ctctttccac tactgggtgc ctagattcca ctaattctaa tgttttattt tcactaaatg
    16201 aaggcctagt gtaattatat ttttgagcaa tttctgcaaa gctctgtaaa caatctagct
    16261 ctgaaataat tttagcttgt tgttgtaaac gttcagtata ttttttaact tcttcacgta
    16321 gctgaacaaa taattgatat tctaattcga tggctttgtc ttccgcacct aaaatgatat
    16381 cttctttttc tttaagttca tcagttataa aacgttcagc attcgataac gtttgcttcc
    16441 tcatataacc aaattcactt ggttcaaaat tttgcaagtt ggcacgtgtt atttctataa
    16501 aataaccaaa cactttatta aagcttattt tcaatgattt tattcctgta cgttgtcttt
    16561 ctttggcttg taattctgct aaccatgttt ttccgttttt tgaagcttca agatattcat
    16621 ctaattgcgt attaaaacca actttgaata gtccgccatc tttaactgaa attggtggtt
    16681 cttctactaa actctgttct aatatatcaa gtaaatcatc aaggggttct agttgattaa
    16741 cttgtacaag cgtattctga ttcatagaat ttagtaatgc tttaatattc ggtatttcag
    16801 aaatggaatg tttaagttga attaaatctc tcgaattaac atttccgtaa ctaacacgcc
    16861 caacaagacg ttcaatatca tacacttgat taagatatgt tcttaaggtg tctctttcta
    16921 tgaaatgagc actaaattca tcaacgatat ctaatcgtgc ttcaatttgt tctttactta
    16981 ttagtggtct atctatccat tgttttaagc ggcgtgctcc cattggtgtt ttcgtttcgt
    17041 ccattagcca aagtagcgtt cctttttttg attttaatcg aatgctttcc gttaactcaa
    17101 ggtttctctt agcataaaaa tccattttca tataatctat agctgcatat tgaacaacat
    17161 cctcgatatg cgataaatca cgtttttgtg tatgatgaat ataatctagc aataattgtg
    17221 tcgcttgata cattaattta tgttcagttt gattcacact atagatttct gatgataacg
    17281 tttccctgac tgttattgtt tctgtcacca tattaatttg tctttttaaa ttatcggaaa
    17341 tattgtcatt gataacaact tcgttagggt ttatcgtcgt aatttcattt aataaagtcg
    17401 cttcatcatt aaaatgtgta acctttaatt cgccagtaga aacatcacag taactaagcg
    17461 caatttcagg ttgattcata acaaaactta aaatatagtt attttgttta tcatctacac
    17521 caccttgctc catcacagtt cctggagtca caattcttac tacctcacgt ctaaccatac
    17581 ctttcgtttg tttcggatct tccatctgtt cacaaatagc tactttatat ccattattaa
    17641 caagtgtatc tatataacta tctgcagaat gatacggaac accacacatc ggaattggat
    17701 tttctttttt agcatctctt ttagtcaaag taatttcaag tacacgtgat gcctccttgg
    17761 catcttcata aaacatttca tagaaatcac ctagtctaaa aaataataag caatcttggt
    17821 attctgattt tatttttaaa tattgcttca tcattggtgt aacattagac atattatttc
    17881 ttcacaaccc ttgtctcttt ttaaaatttg tctttacaat atattcgttt gtaagctttt
    17941 taattattaa ttatttaact tatacatttt aacatactta cttttacaaa cctattcata
    18001 ccatataatc acgaagcatc ttaaatgtat aagaaaacgc ctcaaaccta ataaaatgtg
    18061 tcaatagcat gtttagaatt aaattaaaat tctaacattc aagacattta attaagtaag
    18121 ggcgttcaat attaaaatga acaatgactc tgtttgaaat catatatcat aaatttattt
    18181 tataaacctt tgaagaatac cacgtttttt tagagtaatt aataagaaat aacttataat
    18241 agatccgata gcacttgaga ctatgaacgt aatcattaac ggtttaatga agaagtcttg
    18301 aagcccgagg aaatatgcta atggtataca aattaaactt ccgatgacac cagttccaag
    18361 tacttcaccg accgcggcca tgaatatatg tttacgatat atgtaaaaca tactagccaa
    18421 taaaactcca atcatactac ctggaaatgc aaaagctgta ccagtaccaa aaagaactct
    18481 taaaattgat gatataagcg cttgagctaa tccataccaa ggacctacta tgaccgcact
    18541 taatacattt acaaaatgct gtactggtgc tgccttaact ggtcctagag gaatgatgat
    18601 aatactgctt aatacaacat ttattgcaat taaaagtgca gttatagcca gttttcttga
    18661 tttcatatga ttgttctcct ttttgtttgt aattaatcac tatgtttggc tttattatgg
    18721 tcatttaaac gtgtttccat tgttgataca aacattttca ataattgatt cgcttcatat
    18781 tgtgaagttt gaaactgttc aactatgggc aatgtattta tttctgcttc tatactttga
    18841 atggtatgtt ccgactgttc tagcgcattt tgtttcccgt aattttgaaa gtttactgct
    18901 tgtttttgat gcttttttaa catatccatt ttagtcttta tcgtttggtt ctgatgaatc
    18961 tgtgcttcaa tttgttgata tgttttgata gtatccaaat ttttaatttt atttgcaata
    19021 ttatccgctt gtttcaacac gtcatcttta ttatacattt attgaatcac catttccggc
    19081 tctacttcct ttacaaaact gccatttaat gaatactgtt tagcttcatc tattcgtact
    19141 tccactagtt taccaatcat ttctttaggc gctttgaaat taactagctt atttttatca
    19201 gtgtagccag caagaacctg atcatctttt ttactactac cttcacaaag tactgttaca
    19261 gtttgtcctt cgtacttact catagctatt tgtgaataat gaccaacttt tttattcaaa
    19321 cgttgcaatc gttccttttt gacatctaat ggtacattat ctttcatttt agcagcaggc
    19381 gtaccatcac gttgtgaata caagtacgta tatgcatgtt caaaaccaac ttcatcatac
    19441 agagttaaag tttcttcaaa ttgttcctct gattcatttg gataccctac aataatatct
    19501 gtagttaatg ctacattagg aattctatct ttgattcgtt ttactaaatc caaataactt
    19561 tctcgtgtat attttctacc cattattttt aatactgcat tatttccaga ttgaactggc
    19621 aagtggatat gcggaacgat attaccaccc tctgaaataa catcaatcat gtgatctgta
    19681 aagtcccaag gatgacttgt tgtgaaacga actcttggaa tcgctatttt agaaattgct
    19741 tgtaaaagat ctcctaagtc atattctata tcctgtaaat ctttaccata agaatttaca
    19801 ttttgaccta aaagcgttat ttctttgtaa ccttcacgag caagttcacg tacttcatct
    19861 ataatgtctt caggtctacg gcttcgttct ttacctcttg taaatggaac aatacaatat
    19921 gtacaaaact tatcacaacc atacataata ttgacccatg ctttaatgtt gccttcacgg
    19981 acttttggaa gattttcaat aacgtctcct tctttagacc atacttcaac aaccattgct
    20041 ttagataagt atgcttcttc taaaatttct ggtaaatgat gaatattatg tgtaccaaat
    20101 atcatatcta cattttgata cgattttaaa attttattca ctactgactc ttcttgtgac
    20161 atacaaccac aaacaccgat taaaatatca ggtcgttctt ttttcaaatg cttcaaatta
    20221 cctatttcac taaacacttt gttctcggca ttttctctaa tcgcacatgt attaattaaa
    20281 ataacatctg cagtgttaat atcagtcgtt gcttgatagc ctaatgcttc aagtatacca
    20341 gcaatgacct cagtgtcatg tgcattcatt tgacatccat atgttttaat taaaaatgta
    20401 cgctcgttcc ccataccgcg atatttttca tcaatttgga aatctctatt ataacgaact
    20461 tcttgtttac ctcttttttt cgcttctttt aaattaggcg gctgataaac atgttcaaaa
    20521 tatttactat aatctttttc tgctttctta tctctctcag ctaaaacatc tacagaactt
    20581 gcttttcttt gttcttcgtt cactttacaa aatcctttct atttgcccgt atcatttaat
    20641 aattatatta aatttaacta taaagtgcaa aaacagttaa agttatacgt catctctaac
    20701 aagccaattc aaatccagtt tacttcactt ttaatgttat tcatcatatt tttcagttaa
    20761 agtttcaata gtgatgccat ctggcacatg ataaaactta acttggctaa taatacttgg
    20821 acattcaagt tccaccattc gttcatttaa tgattgtatt aaaattaaac attcattcat
    20881 atttccttgt actgtcgttt ctaacggacc tactctaaaa tgcataccag attcgtcaat
    20941 tattttaatc gcctcgtcta cataaggtat aacattgtca ttgtttggtg ttttaggaat
    21001 tatttgtata ctcattaatg tgtctttcat ttttaaaaac tcctttattt tttgtaatag
    21061 taaaaaactg agtagcataa atggatctgt tataaataca tttatacgac tcagttttaa
    21121 ttattaaata aattgttcag ttaatgcatt gaaggtgtct tcagtaattt tgatatctct
    21181 tttagcaagt ggtgtatcat ctaactcttt aatttgagat tcatatgatg gtgtttcttt
    21241 atcttgataa acaataccag ttactaaaga ttcatgttca ataacagttc tagtcgctaa
    21301 ttgtttatct gtagaatcat aattttcaat gtcatcaaca cttgttaaat gttctttaaa
    21361 ccaatcgtat gtgttaattt tattataagt cacacatggt gaaaagacat taacgaatga
    21421 aaatccatca tgattaattg catcttcaat tagttttgtt aatcctttaa tatcacttga
    21481 aaaaccttgg gctacaaatg tggcaccaga tgataatgct aattctaaag gcgcaacatt
    21541 tttttctata ttaccttttg gcgttgtttt agtaacaaat cctactgctg atgacggcga
    21601 tgtttgtccc tttgtcaaac cataaatttg attatccatg actatatacg tcatgttcat
    21661 atttcttctt aaagcatgga ttgtatgccc catacctata gcataaccat caccatctcc
    21721 tccagatgca ataacagtta aatctttatt cgccattttt acaccttgag ctaaaggtaa
    21781 tgcacgtccg tgaatagaat gaacgccata agaattaata tatcctgaaa gacggccaga
    21841 acatcctata ccggtgatga tagctacttc ttcaggttct aaccctatat ttgcggctgc
    21901 tttttgaatt gcagcttgta ctgagaaatc gccacatcca gggcaccagt taggcttaac
    21961 attatttcta aaatctttaa atgtcgccat ctataccatc tcctttattt cagtagcaat
    22021 tattttgcct ttttcttcga tttcatgtgg taggaaaggt gtcccatcat actttgtata
    22081 attttcaatc ttatcatgaa tattgacatt catttttata atactagcca attgtccttg
    22141 ataattgtgc tccactacaa cgactttctt cgctttatta actgcatctt gaataacgct
    22201 tgttgggaat ggatgcaatt gtctaatttg tatagtgtta acttttatgc cttgttgatt
    22261 caaacggtta ctaccttctt gaattgcacc ttttgtagaa ataaaaccga tataaagaat
    22321 atctgcatcc tcatgttgta agttagcttc tactggcgat tcaattagta actgctcaat
    22381 tttacgcatt cgtttttcca tttgttgttg tctatttgac gcagattcac taggtttacc
    22441 ttcttcattg tgttccacac cagttatatg atgaatacct cctttaacac cggggatagg
    22501 tctaggagaa acaccattgg atgttaacgc ataacgcttg aaataacctt tatcatcttc
    22561 ttcacgttca atatcagatt gaatgatttc accacgttta atttcaatgc gattataatc
    22621 taatttttca acagtttgtt tacctaaaga taattgcaaa tcacttaaaa ctataacagg
    22681 gcattgatat tgttctgcta aattaaaggc ttccatagtt aaataaaatg catcttctgc
    22741 atcagttggt gctacaacta tttttggaat atcaccatgt gtaccataaa tcatttgcat
    22801 taaatctgac tgttcttgtt tcgtaggtaa tccagtagaa ggtccacctc gttgagtatt
    22861 aataatgact aatggcgttt cagtcatacc agataatcca attgcttcca tcattaaaga
    22921 taaacctgga ccagcactag ccgtaaacgc tctgactcca ccataatttg caccaatagc
    22981 catagttaca gcagcaattt catcttctgt ttgaataacc gctccgttta ctttagatat
    23041 attggcaatc atatattcca taacttcaga cgcaggtgta ataggatatg ccgccataaa
    23101 ttgtgaacct gcagcaattg cacctaatcc aatggcatcg ttaccaatca tatatagatg
    23161 tggtagtgca tctgttgact ctaattcaaa gtccccgtcg atttcaggta agcgggattg
    23221 cattaattga taaccttcgt ttaatgcttg gatattgact tcaactacct tgtcaccttt
    23281 tttagaaaac atattagtaa taagttcttc aaatgtattt gtattcaaat tcatcaatgc
    23341 gctagtagca cctattgcaa ccatgttttt cattaatgct gtacctaatt ctttagcggt
    23401 tgctgtaaaa ggtaattcaa taagctgtgc atgacatcct tctggtttca caggtttagc
    23461 cttggcatca gctaaaataa tactgtcttc tctcatttca tgatggttaa catcaattgt
    23521 ttcttggtca aatgcaatca aaatatctaa atcatcacta attgcatgaa caggcgtcgt
    23581 agaaactcta attttattat tcgtatgtcc acctttgata cgacttgaaa aatgtctata
    23641 tccatataaa taatatcctt ttctattcat agccgtagcg aagatttccc cagttgattc
    23701 aataccttcg ccttgttgac cgccaacttt ccacgataat tgtggtttca tagttatatg
    23761 cctcctatgg atatatttca tattaaatga taacaaaaag aaccaccgat ttactatgta
    23821 aacagtggta ctatattaaa ttcatactaa tggacgatag ggtccaattc gaatgtttac
    23881 ttacgtaatt ttaaaatgtt gaaaatggat ggtcatcatt tatcaatata cgttcgatgt
    23941 gctttgtttt accttctttg tctaaatcaa taacaacacc agataataca cttctacctt
    24001 catttggaac aacatgtctt tgtggcaaac tagtgataaa acgctcaatt acctctgttt
    24061 tatttattcc taaaatgcca tcataaaaac ctgtcatacc aacatccgtt atataccctg
    24121 tcccctttgg taaaatacgt tcatctgctg tttgaatgtg tgtatgcgtt ccaacaacag
    24181 cgctagctct accatctaaa tgccatccca ttgcatactt ttcagaagtt gtttctgcat
    24241 gaaaatcaac aaatataaac ggagtttgtt cttgtgcttc cttgactaat tgatctgcct
    24301 ttttaaaagg atcatcaata tctggcataa acgctcttcc ttgcagatta ataactgcaa
    24361 gtttaatatc attaatttgt ataaatctca taccaattcc cggcgcttca tccggaaaat
    24421 tcgctggtct tactagtcgt tttgcttcat ctataaaatc ataaatttca cgttgaccat
    24481 atgtgtgatt acccatagtc atgaaatcta caccatttct tagtaattgt ttatatattt
    24541 tttcagtcaa acctttacca tgtgctgcat tttcagcatt tacaattgta actgttggtt
    24601 tatacttttg cttcagttga ggtatgtacg tttcaattgc gtctcgtcca attttaccta
    24661 cgatatcccc tataaacatt attctcatgt tttgttcatc ctctcataaa tact
//
LOCUS       DAHHYJ010000024        24423 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000024 DAHHYJ010000000
VERSION     DAHHYJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 24423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24423
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.024"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..134
                     /locus_tag="NBK22_000738"
     CDS             <1..134
                     /locus_tag="NBK22_000738"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HCD2413941.1"
                     /translation="EVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
     gene            162..1340
                     /gene="deoB"
                     /locus_tag="NBK22_000739"
     CDS             162..1340
                     /gene="deoB"
                     /locus_tag="NBK22_000739"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCD2413942.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(1471..2286)
                     /gene="phnE"
                     /locus_tag="NBK22_000740"
     CDS             complement(1471..2286)
                     /gene="phnE"
                     /locus_tag="NBK22_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCD2413943.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(2283..3083)
                     /gene="phnE"
                     /locus_tag="NBK22_000741"
     CDS             complement(2283..3083)
                     /gene="phnE"
                     /locus_tag="NBK22_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCD2413944.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(3085..3858)
                     /gene="phnC"
                     /locus_tag="NBK22_000742"
     CDS             complement(3085..3858)
                     /gene="phnC"
                     /locus_tag="NBK22_000742"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCD2413945.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            complement(4072..5028)
                     /locus_tag="NBK22_000743"
     CDS             complement(4072..5028)
                     /locus_tag="NBK22_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD2413946.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5257..6801
                     /locus_tag="NBK22_000744"
     CDS             5257..6801
                     /locus_tag="NBK22_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD2413947.1"
                     /translation="MKKIYKSLTVSAIVATVSLSALPQSLAITHESQPTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKQGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITAWMSKDNDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKAGSYGSEKGADP
                     QPNTPDDHTSPNQNEKVSFDIPQNVSVNEPFEVTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNATIKINAKVLESMEGSKIRLKLGDKTL
                     ITTDFK"
     gene            6852..8387
                     /locus_tag="NBK22_000745"
     CDS             6852..8387
                     /locus_tag="NBK22_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD2413948.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFETLQQHLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     GFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGKVVLSTDEYENVSVESCELL
                     PVIDDSTFTIDEDDQHIRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     FLEKSGADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIGRSA
                     EYFDVKNDKVSVSADFLEPKPQHFNYDIYGGVSYTINVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
     gene            8544..9311
                     /locus_tag="NBK22_000746"
     CDS             8544..9311
                     /locus_tag="NBK22_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413949.1"
                     /translation="METIKQEVRESIVKGIIAGYKDYVNERNEVKKRMVISDAYVYTK
                     SNHIENQVAKHLENFVKYIKENAGPSWKYLKFIFNKDEEKNNIMFILKNEDYFDEKNV
                     SVGKSLVADKNPKSKNYLEVLMAKNRDINFGTVGEDFEIGHQMTADSILFNIKEGSNR
                     DINSYFLIITYRIDKESKQLAAIKQWLPNPETNSAIMVDDLTELIEKVIVEREDYHID
                     EEELEVLKNDGELELIDVEHAFGISIDEGNDMIESER"
     gene            9317..10492
                     /locus_tag="NBK22_000747"
     CDS             9317..10492
                     /locus_tag="NBK22_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCD2413950.1"
                     /translation="MFIGKNLEYVRKLNALSRKELSEKINVSEQAIWQYETKNMMPEI
                     SKIYDMTSIFNVKSSYFISEQPEELLINSVDKHSIAFRAKNYKVSTKLLNKQYYQAMY
                     LSNLTSYLFSFVTIPDNIILSLINNLDELLNGNLESLNKKESIKEIAKVVRAKILQDE
                     SNEALLFMLEKAGIVIYEKRINDSIDAYSFWSKDQTPFIILGTNKGVAVRRNFDLAHE
                     LGHLVLHRHIQFDLLSPEEYKTIEHEADIFASEFLLPEEAFKKDFDQMTKKSNPDYLA
                     VLKEKWYVSIQAIAMRAYYLGLMSSTQYRYFWASLNKKGYKSKEPLDDVIEMSRPVKM
                     NSLLKLYFDRNILTPQKLLNYLKVDETFLNHLAGINLKLFKDYVNENREYNITNLYK"
     gene            10877..13486
                     /gene="adhE"
                     /locus_tag="NBK22_000748"
                     /gene_synonym="adhC"
     CDS             10877..13486
                     /gene="adhE"
                     /locus_tag="NBK22_000748"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCD2413951.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTTMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
     gene            13831..14499
                     /gene="capA"
                     /locus_tag="NBK22_000749"
     CDS             13831..14499
                     /gene="capA"
                     /locus_tag="NBK22_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCD2413952.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            14515..15201
                     /gene="cap8B"
                     /locus_tag="NBK22_000750"
     CDS             14515..15201
                     /gene="cap8B"
                     /locus_tag="NBK22_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCD2413953.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLVIDGDMRKPTQNYIFNEQNNNGLSSLII
                     GRTTMSEAITSTEIENLDLLTAGPVPPNPSELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKALMEKAGSNILGVILNKTKVDKS
                     SSYYHYYGDE"
     gene            15204..15968
                     /gene="cap8C"
                     /locus_tag="NBK22_000751"
     CDS             15204..15968
                     /gene="cap8C"
                     /locus_tag="NBK22_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCD2413954.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMLDLLKQATTQGVSEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            15988..17811
                     /gene="cap8D"
                     /locus_tag="NBK22_000752"
     CDS             15988..17811
                     /gene="cap8D"
                     /locus_tag="NBK22_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HCD2413955.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGKIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEIMETYKPYAVYHAAAHKHVPLMEDNPEEAVHNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHRTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRPGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            17801..18829
                     /gene="cap8E"
                     /locus_tag="NBK22_000753"
     CDS             17801..18829
                     /gene="cap8E"
                     /locus_tag="NBK22_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCD2413956.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFVAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            18842..19951
                     /gene="cap8F"
                     /locus_tag="NBK22_000754"
     CDS             18842..19951
                     /gene="cap8F"
                     /locus_tag="NBK22_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCD2413957.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDHHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNKKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLRQYAEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            19955..21079
                     /gene="cap8G"
                     /locus_tag="NBK22_000755"
     CDS             19955..21079
                     /gene="cap8G"
                     /locus_tag="NBK22_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCD2413958.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QKNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            21082..22161
                     /gene="cap8H"
                     /locus_tag="NBK22_000756"
     CDS             21082..22161
                     /gene="cap8H"
                     /locus_tag="NBK22_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8H"
                     /protein_id="HCD2413959.1"
                     /translation="MRIAILGATNIKHMSLLSHYLNHIDLNINEVDIIYTDKYDIEEH
                     IQGINNYYKYKVDIKEDWTFIKKAIAYYRFRPYAMKILKENRYDFVIVWGSYTGHLFK
                     SFLEKHYKNKFILNIRDYFFENNKLIKYRMKKIVDASRVTTLSSEGFLKFLPKSEKYR
                     IIYSYNMSIIRESNVTDGFKKRWPINIGFIGNVRFNEINQKLIKELANDSRFHMQYFG
                     TGSEKLEVFARENFINNITFSGGFDLKETPKYLNEIDILNNLFGNQNIALDTALSIRM
                     YYALFLNKPIITTDDTFTATEANKFGLGFSINPENLKGIGDELMDWYNNLDVMDINHK
                     REAYRNDVIENNKQFYQEIGRIFNE"
     gene            22154..23548
                     /gene="cap8I"
                     /locus_tag="NBK22_000757"
     CDS             22154..23548
                     /gene="cap8I"
                     /locus_tag="NBK22_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8I"
                     /protein_id="HCD2413960.1"
                     /translation="MNKIYNVTSYVIAILMFPCLMLGDKPLLFLAPISYGVGKLFISF
                     SYNPNFKFSKIVYDVLGFLRLVFIPAMIVFFQDSTIDNLPLGQAYFNQAVIYMSVEFI
                     IGSLFILILSKLFKHEVVSRNSFTLSGSSIYYIVFGLVICGIFVAFPEVRKNISFLII
                     KTDAMGRGTEATSGLNVLFVMLFQLALALLFLIIAYASYKKYKENPKIIYVVLPLAIG
                     ILNISLIVGERRSYQLYTMVAVLTVVSILFSKHKRRINIIIISVGIFVLALMTLYKEL
                     YVFNYSSYSEALNSTSVSNLKIVDTLQSYFYGPSNIAASIDYLNYYNGSFKQYLFDNT
                     RAVFGFNFFLDKKQLITSQLFNQLIYGSKQLTGHLISSAGYGIIYFGPLFFYLNLIAN
                     IFFAFLSEYIIRKSHSLEVIFIGTYIYMRLITSIFSHPTPLITLISMILVVYVIAIIP
                     GIIIKKFTKKVGIE"
     gene            23545..24102
                     /gene="cap8J"
                     /locus_tag="NBK22_000758"
     CDS             23545..24102
                     /gene="cap8J"
                     /locus_tag="NBK22_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8J"
                     /protein_id="HCD2413961.1"
                     /translation="MIVKTFMKSKIFRLMNTPLLLFYKKEYLTGYYFENKVAGWLWAW
                     KAVPFKLLGINMSLPFPADITVRMHNPNNIVFDKNDIHIFQSPGTYFNNFSAVIYIGR
                     GVYIAPNVGIITANHNIKNLKSHAPGEDVKIGNYSWIGMNSVILPGVELGEHTIVGAG
                     SVVTKSFPEGNVVIGGNPAKIIKKI"
     gene            24111..>24423
                     /gene="cap8K"
                     /locus_tag="NBK22_000759"
     CDS             24111..>24423
                     /gene="cap8K"
                     /locus_tag="NBK22_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8K"
                     /protein_id="HCD2413962.1"
                     /translation="MRLNKFIGDSFLMILSSGIAQVILIITTPIITRLYSPTEFGEFT
                     IFSNIAMILIPIINARYDLLIVNTKNDRSANILSQISFLISLLILLILIPIFAISACL
                     YP"
BASE COUNT         8709 a         3537 c         4525 g         7652 t
ORIGIN      
        1 tagaagtaaa agcatcaggt ggcgtacgta atttagaaga tttcaataaa atggttgaag
       61 caggtgcgac acgtattggt gcgagcgcag gtgttcaaat tatgcaaggt ttagaagcag
      121 attcagatta ctaatatata taaattttgg gagtgatagc tatgacaaga ccatttaatc
      181 gtgtacattt aatcgtaatg gattcagtag gtattggtga agcgccagac gcagctgatt
      241 ttaaagatga aggttcacat actttaagac ataccttaga aggtttcgat caaactttac
      301 caaaccttga aaagttaggt ctagggaaca tcgataaatt accagtagta aatgcagttg
      361 aacaaccaga agcatactat actaaattga gtgaagcttc agttggtaaa gatacaatga
      421 ctggtcactg ggaaattatg ggattaaata ttatgcaacc ttttaaagta taccctaatg
      481 gattccctga agagttaatt caacaaattg aagaaatgac aggtcgtaaa gttgttgcta
      541 acaaaccggc atcgggtacg caaattatcg atgagtgggg cgagcaccaa atgaaaactg
      601 gtgacttaat tgtttataca agtgcagacc cagtattgca aattgctgca catgaagaca
      661 ttatcccatt agaagagtta tatgatattt gtgaaaaggt tcgtgagttg acaaaagacc
      721 ctaaatattt aattggtcgt attatcgcac gtccatatgt tggtgaacca ggaaacttta
      781 cacgtacatc taatcgacat gactatgcgt taaaaccatt tggtaaaact gtcttagatc
      841 atttgaaaga cggtggttat gatgttattg ccatcggtaa aattaatgac atttatgatg
      901 gtgaaggtgt aacagaagcg gttcgtacga agagtaacat ggacggtatg gatcaattga
      961 tgaaaattgt taagaaagat ttcacaggta ttagcttctt aaacttagta gactttgatg
     1021 cattatacgg tcatcgtcgt gataaaccag gttatgcaca agcaattaaa gatttcgatg
     1081 atcgcttgcc agaactgttt agcaacttaa aagaagacga tttagtaatt attacagcag
     1141 accatggtaa tgacccgaca gcgccaggta cggaccatac gagagaatat atcccagtaa
     1201 ttatgtacag tccgaaattt aaaggtggtc atgcactaga aagtgatact acattcagtt
     1261 ctatcggtgc aactatagca gataatttca acgtaacatt accagagttc ggtaaaagtt
     1321 atttaaagga attgaaatag aataaattta gatattataa aaacagcagt gaagttaact
     1381 ataacaatag ttttcttcac tgctgttttt attataatag agaaacgtaa gacggtagga
     1441 cttcttattt aggagtatcc tgatttaatg ttaaacaata cgttttcgga ttgaaccgga
     1501 aattaaatcg acaattgcga ccattagtac taaaccgatt aatataatac ctacacggtc
     1561 ccaagaacgt gtttgaatgg caaatatgag tggtgtcccg ataccaccag ccccaattag
     1621 ccccagtata gaagctgaac gtaagtttag ttcaaagcga taaagtatga gtgatagaaa
     1681 ggcaggcata atttgtggta tgactgcaaa tacgagtgtt ttaatcttat tcgcaccact
     1741 ggcctttaat gattctacag cactgaaatc tagaccttca atatcttcag ctaaaagttt
     1801 cccaagcata cctacggaat ggatacctaa agctaataca cctgaaaatg aacctgggcc
     1861 aacagctttg ataaatataa gtgccattac aatttctggg aagacacgta taacacttaa
     1921 aataaatttg ctaacacctg aaactgggcg tagctttacc atattatttg cacctagaaa
     1981 tgctaatgga atacagataa ttgcggcgat gaaagtacct acaacggcta tcgcaaaggt
     2041 ttcaagtaaa ccacgtaata agtcttcgcc atctggtata tagatatagc tgatatcagg
     2101 atggaataat ccgctgaata tggattttaa gatttctaat gatttacttt taagttctaa
     2161 acttggtaca cctgcaaatg cccagatgat aatagctaag acgacaattg caataagcca
     2221 tcttttaatc aattttcgtt tgtgtgcttt tgtgtgaaca ttatattttg ctatttcctg
     2281 tgtcatgcga gatgtgccct cactttcgta ctgatgtaat caatgacgac gacgataact
     2341 aaagtaaata aaataatcgt tgctgttttt ggatattgaa ataaaccaag tgtttgatca
     2401 taaaacaatc caataccgcc agcgccgact aatccaagca cagctgaagc acgtatattt
     2461 acttcaaatg catataatac gtatgacata aatgacgata tggcttgtgg tacaacaccg
     2521 aaaacaatcc attttatttt attagcgcca acagccgtca ttgcttccat tggacctgga
     2581 tctatcgttt ccaatgattc atataataat tttccaataa tacagatagt taaaataaac
     2641 agtgctaata tccctggaat ttgaccgatt ccaaatacag ccacaaagat tgctgctaat
     2701 aacaaatctg gaatagtacg aactatattt aaaataaagc gcgagggtat tgaaatccac
     2761 ttttgatgaa cgatattgct agcacataat aacgcaattg gtattgaaac gatgctacct
     2821 aatactgtac ttacgatagc cattcgaatg gtatctaaca ttggcgttgt aatttgttgt
     2881 aaatactcga aatcaggtgg aatcatttgt ttgaatagat cacctatttg aggtattcct
     2941 atcattaaat ctccaaaatt aaatcccgta taaatgaagc tccaaatgat aagcacaatg
     3001 attaacatga aggtaaaact cgtttttaaa gaaacctttt tctttaaaag ggagtcatac
     3061 tttgtaggta tttctaaagg catgttagtt cactcctagc ttttcatctt ctttaattgt
     3121 acgtccatat atttcactaa atacgtcatc tgttgcttca gatgcaggac catcatagac
     3181 aacttcacca tcacgtaaac caatgatgcg tgtgccatat tcttttgcca agtcaacaaa
     3241 atgtaaatta attaaaattg tgatgcctaa ttcttggttg atttttctta aatcatccat
     3301 aacctgtttc gtagttaatg ggtctaatga agcaactggt tcatctgcaa gaataatttc
     3361 agattcttgg catagcgcac gtgcaataga tatacgttgt tgttggccac ctgataattc
     3421 atcagagcgt tgattatatt tatctaagat attgacgcgt tctagtgcat ccattgcttt
     3481 aattttgtct tcttttggga ataaacctaa taccattttc caagtagggt gataacctac
     3541 acgtccactt agtacatttc gtaatacact tgaccgttta actaaattaa aatgttggaa
     3601 aatcatacct atatttcggc gcatttctaa taatgcttta ccatgggctt tagtgatgga
     3661 tttaccttgg atgaaaattt cacctgacgt gatatcatgc aaacgattta cagatcttaa
     3721 taacgtggat ttcccagcac cagatagtcc gacaataact gcaaattcac ctttttcaat
     3781 atttaagtta atatttttca agcctacatg accgttagga tagactttac tgacgttttt
     3841 aaattcgatt tgactcatgt ttgacacctt tctttaataa agaaaagcca aggtaaactg
     3901 aaatatcata gacattgggt ttctgtgtac aaatggtttc aaatgtacag cgacaccttt
     3961 ctatatctgt ttcagtttag cgcctagctt ttcaatatta atctagaata tatctattga
     4021 acgaaagctt ttaataccaa attcgctaat gattcatttg ttaaataatg attatttcat
     4081 atctttaact aatttttcgt actctcttac aatgtcgaaa tttgaatctt tcgtttctgt
     4141 gtatccttca tgtgaataaa cttcgctaat aattttgtga ccttcttttg atttagcaat
     4201 gtctataaaa gcttttttca atttttcttg aaaatcttta tccatatctg gtcttacaga
     4261 aattgtgtca ttcggaatag cttgtgttaa ttttaaaatt cgtgtgtctt taaatacatt
     4321 tggttggtct tttttcacag tattacgtgc atcgttaaat acagctgcag catctacatc
     4381 tccatttaat aatgagataa ctgcttggtc atgaccttta acattcacaa ttttcatatc
     4441 tttagttgca ttaatacctg cttcgttttt taacatcgca agtgggaatg tatatccagc
     4501 agttgatgtt acatcttgta aggcaatttt cttacctttt aaatctttca agcttttaat
     4561 ttttgagtct tttttaacaa gaatttctga tttatagcta tctacaagtt ctttacttgc
     4621 tgaaccatct tcttttacac cgaaacgttg tgcttgtaat aataaatcag ctgctttttg
     4681 atcatgtgct aatgtgtatg ccgttggtgg taagaaacca acatcaactt ttttagactt
     4741 catagcttca acaattgtat tgtagttagt tgatacagac actttaactg gaatccctaa
     4801 ttctttagat agtaattttt ctaatggttt tgctttagct tctaatgttc cagcattttg
     4861 cgaaggtaca aattgaacgg ttaattcttt aggtttgtat cctcctgatt tagaatccga
     4921 atcattacta gcgttctttt gattatctaa agaacttgaa tttccacatg ctgctgcaaa
     4981 aacaatgact gctaacatta atacaaataa acacttaaaa tttttcattt gataactgtc
     5041 cccttcacat tcttaatcgt gtaatatgta tgcaatgaaa ttacacttat agcgtaccac
     5101 atttaaatgc gaaaaagtat tttataaaaa tgaattttaa taactttctt taagagtttg
     5161 ataaggtttt gtaaattttt cgttaaattg cccatatgca acgaattttt atcctataat
     5221 tcaagaataa atttatttta aaaggagcct cacaaaatga aaaaaatata taagtcatta
     5281 actgtctctg caattgttgc aacggtatca ttaagtgctt taccgcaatc tttagctata
     5341 acgcatgaat cgcaacctac aaagcaacag cgaacggtat tattcgatcg ttctcatggt
     5401 caaacagctg gtgctgcaga ttgggttagt gatggtgcat tttcagatta tgcggattca
     5461 atacaaaaac aaggttatga cgttaaagct attgatggtc attcgaacat aacagaagca
     5521 agtttgaaaa gttccaaaat atttgtaatt cctgaggcta acattccttt caaagaatca
     5581 gaacaggcag caattgttaa ctatgtgaaa caaggtggca atgttgtctt tatttcagat
     5641 cattacaatg ctgaccgaaa tttaaatcgt attgattcat cggaggcaat gaatggttat
     5701 cgacgtggag catatgaaga tatgtcgaaa ggtatgaatg cagaagaaaa aagttctact
     5761 gcaatgcaag gtgtgaaaag ttcagattgg ttatctacaa actttggcgt acgttttcga
     5821 tataatgcac taggtgattt aaatacgagc aatattgttt cttcaaaaga aagtttcggt
     5881 attactgaag gtgtgaaatc tgtctctatg catgccggat cgacattagc aattactaat
     5941 ccagagaaag caaaaggtat tgtgtataca ccagaacaat tgccagcgaa aagtaaatgg
     6001 tcacatgctg tagatcaagg tatttataat gggggcggta aagcagaagg cccctatgta
     6061 gcaatttcta aagttggaaa aggtaaagca gcatttatcg gtgattcatc acttgtggaa
     6121 gatagttcgc ccaaatatgt aagagaagat aatggagaaa agaagaaaac atatgatggt
     6181 tttaaagaac aagacaatgg taagctatta aataatataa cggcttggat gtctaaagat
     6241 aatgatggga aatcacttaa ggcgagtggc ctaacattag atacaaagac taagttgctt
     6301 gattttgaac gaccagagcg ttcaactgag cctgaaaaag agccatggtc acagccgccg
     6361 agtggttata aatggtatga tccaacaaca tttaaagcag gtagttatgg cagcgaaaaa
     6421 ggcgcagatc ctcagccaaa cacaccagat gatcatacgt caccaaatca gaacgaaaaa
     6481 gtatcatttg atatcccgca aaatgtttct gtaaatgagc catttgaagt gacaatacat
     6541 ttaaaaggat ttgaagcaaa tcaaacactt gaaaatctta gagttggtat ttacaaagaa
     6601 ggcggacgtc aaatcggaca attttcaagt aaagataacg attataaccc gccaggttac
     6661 agtactttgc caacagttaa agcagatgaa aacggaaatg ctacaattaa gatcaatgct
     6721 aaagtacttg aaagtatgga aggttcaaag attcgtttaa aactcggtga caaaaccttg
     6781 attacaacag acttcaaata aatatataat agaaataaga aagatgtttg tgattgaagg
     6841 agtgagtgag gatgtcaaac atagcatttt atgtcgtgag tgacgtacat ggttatattt
     6901 tcccaacaga ttttacgagt agaaatcaat atcagcctat gggattgtta ctagcgaatc
     6961 atgttataga acaagacaga aggcagtatg accaaagttt taaaatagat aatggtgatt
     7021 ttttgcaagg gtcaccattt tgtaattact taatcgcgca tagcggcagt agtcagcctt
     7081 tagttgattt ttataatcga atggcattcg actttggtac gcttggtaat catgaattta
     7141 attatggatt accatactta aaagacactt tacgcagact caattatcca gttttgtgcg
     7201 ctaatattta tgaaaatgat agtacattga ctgataacgg tgtgcagtat tttcaggttg
     7261 gagatcaaac ggttggtgtg ataggtttaa cgacacaatt tattccccat tgggaacaac
     7321 cagagcatat tcagtcactt acgtttcata gtgcttttga aacacttcaa caacacttac
     7381 ctgaaatgaa gcgacatgca gatatcattg tggtttgtta ccatggtgga tttgaaaagg
     7441 atttagaaag tggtacgccg accgaagtgt taacgggtga aaatgaagga tatgccatgt
     7501 tagaagggtt ttctaaagat atagatatct ttattacggg tcaccaacat cgacaaattg
     7561 ctgaaaggtt taagcaaacg gctgtgattc aacctggtac gagaggtaca actgtaggca
     7621 aagttgtctt gagtactgat gaatatgaaa atgtatccgt tgaatcatgt gaattacttc
     7681 ctgttataga tgattccaca tttactattg atgaagatga ccaacatata cgaaagcagt
     7741 tagaggactg gttagattac gaaattacta cattgccata tgatatgacg attaatcatg
     7801 catttgaggc acgtgtagct ccgcatcctt ttactaattt tatgaattac gcttttttag
     7861 aaaaaagtgg tgcagatgtt gcatgtacag ctttgtttga ttctgctagt ggtttcaagc
     7921 aagtcgtgac gatgcgagat gttattaaca attacccatt tccaaataca tttaaagttt
     7981 tagctgtaag tggtgccaaa cttaaagaag ccattggacg atcagcagaa tattttgacg
     8041 tgaaaaatga taaagtaagt gtaagcgcag acttccttga acccaaacca caacacttta
     8101 attatgatat atatggtggc gtaagttata ccattaatgt tggcagacca aagggacaac
     8161 gtgtgagtaa tatgatgatt caaggtcacg cagttgattt aaagcagaca tatacaattt
     8221 gtgtaaataa ttatcgtgca gttggcggtg gtcagtatga tatgtatatc gacgcgccag
     8281 ttgtaaaaga tattcaagtt gaaggcgcac aattacttat tgatttttta tcaaataata
     8341 atttgatgcg catcccgcaa gttgttgatt ttaaagttga aaagtgacgg atatatgtaa
     8401 aacttacttg attttaatcg ggtaagtttt tgtttttgtt taaactaatc actcttaaaa
     8461 accaatatac tttaagaaaa atgttgattt tacaaaaagt aaggcttata ttttatataa
     8521 cttaatatta ggaagtgatt aatttggaaa caattaaaca agaagtaaga gaatctattg
     8581 ttaaaggaat tatcgcggga tataaggact atgttaatga gagaaatgaa gtcaagaaac
     8641 gtatggtgat tagtgatgcc tatgtgtaca ccaaatctaa tcatattgaa aatcaagttg
     8701 ctaagcattt ggaaaatttt gttaagtata ttaaagaaaa tgctggacca tcatggaagt
     8761 atttaaagtt tatatttaat aaggatgaag agaaaaataa tatcatgttt atattgaaaa
     8821 atgaagatta ctttgatgaa aaaaatgtaa gcgtagggaa gagtttagtt gctgataaaa
     8881 atccaaaaag taaaaactac ctagaggtac ttatggctaa gaatagggac attaattttg
     8941 gtactgttgg agaagatttt gaaattgggc atcaaatgac tgcagatagt atattattta
     9001 atataaaaga aggcagtaat agagatatta attcttattt tttaattatt acatatcgta
     9061 ttgataaaga atcaaagcaa ttagctgcaa tcaaacaatg gttaccaaat ccagaaacaa
     9121 attctgcaat tatggttgat gatttgacag agttaataga aaaagtaata gtagaaagag
     9181 aagattatca tattgacgaa gaagagttag aagtacttaa aaacgatgga gagttagaac
     9241 tcatagatgt ggaacatgca tttggtatca gtatcgatga aggtaacgat atgattgaaa
     9301 gtgaaaggtg aagttaatgt ttattggcaa gaatttagaa tacgtgagaa agcttaatgc
     9361 tctaagtaga aaagaattat cagaaaaaat aaatgtgagt gaacaagcaa tttggcaata
     9421 tgaaacaaaa aatatgatgc cagaaattag taaaatttat gatatgacat ctatttttaa
     9481 tgttaaatct agctacttta ttagcgagca gcccgaagag ctcctaataa attctgtcga
     9541 taaacatagt atagctttta gagcaaaaaa ttataaagta tctacgaaac tattaaataa
     9601 gcaatattat caagcaatgt atttatctaa tttaactagc tacttatttt cttttgtaac
     9661 gataccggat aatattattt tatctttaat aaataattta gatgaattgt tgaatggcaa
     9721 tttagaatca ctaaataaaa aagaaagcat aaaagaaatt gctaaagttg tgagggctaa
     9781 aattcttcaa gatgaaagca acgaagcact attatttatg cttgaaaaag cgggtatagt
     9841 tatatacgaa aagcgaatta atgatagcat agacgcatat agtttttgga gtaaagatca
     9901 gacacctttt attatactgg gtacaaataa gggggtagca gttagaagga attttgatct
     9961 tgcacatgag ctgggacatc tcgttctaca tcgtcatatt caattcgact tattatctcc
    10021 tgaagaatat aagacaattg aacatgaggc agatattttc gcctctgaat ttttactacc
    10081 tgaagaagcg tttaaaaaag actttgatca aatgaccaaa aaatcaaatc ctgattattt
    10141 agctgtattg aaagaaaaat ggtatgtttc tatacaagca attgcaatga gagcgtatta
    10201 tctaggccta atgtctagta ctcagtacag atatttttgg gcatcattaa ataaaaaagg
    10261 ctataaaagt aaagagcctt tagacgacgt gattgaaatg tcacgtcctg tcaaaatgaa
    10321 tagtttatta aagttgtatt ttgatagaaa tatactgaca ccacaaaaat tattgaatta
    10381 tttaaaagtc gatgaaacat ttttgaatca tctagcaggt attaatttaa aactctttaa
    10441 ggattatgtt aatgaaaata gagaatataa tataacgaat ctatataaat aaggcaccaa
    10501 tatttaaacc tcgagctgaa aagatcatca tttcagttcg aggttttttg tgtaacatct
    10561 gttgataggc agcgacaagg atcttttaat gttgtgtttt gtgcgatata acaagctttt
    10621 taggctatta aagaatattc agaattaaat atataattat gaataaatta tgtcattgaa
    10681 aaaattatga ttataaaatc attctaaaaa caccgaaata acaatgattt catgaaaaca
    10741 tttattttaa aatttgatat ttgttcaaat aatattcgaa attaaacttt tttgtataga
    10801 attttcttta tatcctgaga gacatgtact ataatgtttg tgaaataatt cacaaagtat
    10861 aaaggagtgg ttgtatatgt taactatacc tgaaaaagaa aatcgtggat cgaaagaaca
    10921 agaagtggca attatgattg atgctctagc tgacaaaggg aaaaaagcat tagaagcatt
    10981 atctaaaaag tcacaagaag aaattgatca tattgttcat caaatgagct tagcagctgt
    11041 tgatcaacat atggtgctag caaaattagc acatgaagaa actggaagag gtatatacga
    11101 agataaagcg attaaaaatt tatacgcttc tgaatatata tggaattcaa taaaagacaa
    11161 taagacagta gggattattg gtgaagataa agaaaaagga ttaacgtatg tagcggaacc
    11221 aattggtgtt atttgtggtg ttacgccaac aacaaatcct acgtcgacaa ctatttttaa
    11281 agcgatgatt gcaattaaga caggaaatcc aatcattttt gcattccatc caagtgcaca
    11341 agaatcgtcg aagcgtgcag cagaagttgt attagaagcg gcaatgaagg caggtgcacc
    11401 taaagatatt attcagtgga ttgaagtgcc ttctatcgaa gcaacaaaac aattaatgaa
    11461 tcacaaaggt attgcattag ttctagcaac tggtggttcg ggcatggtta agtctgcata
    11521 ttcaactggc aaaccggcat taggtgtggg accaggtaac gtgccgtctt acattgaaaa
    11581 aacagcacac attaagcgtg cagtaaatga tatcattggt tcaaaaacat ttgataatgg
    11641 tatgatttgt gcttctgaac aagttgtagt cattgataaa gaaatttata aagacgttac
    11701 taatgaattt aaagcacatc aagcatattt tgttaaaaaa gatgaattac aacgcttaga
    11761 aaatgcaatt atgaatgaac aaaaaacagg tattaagcct gatattgtcg gtaaatctgc
    11821 agttgaaata gctgaattag caggtatacc tgtccccgaa aatacaaaac ttatcatagc
    11881 cgaaattagc ggtgtaggtt cagactatcc gttatctcgt gaaaaattat ctccagtatt
    11941 agccttagta aaagcccaat ctacaaaaca agcatttcaa atttgtgaag acacactaca
    12001 ttttggtgga ttaggacaca cagccgttat ccatacagaa gatgaaacat tacaaaaaga
    12061 ttttggacta agaatgaaag cttgtcgtgt acttgtaaat acaccatcag cggttggagg
    12121 tattggtgat atgtataacg aattgattcc gtctttaaca ttaggttgtg gttcctacgg
    12181 tagaaactca atttcacata atgttagtgc gacagattta ttaaacatta aaacgattgc
    12241 taaacgacgt aataatactc aaattttcaa ggtgcctgct caaatttatt ttgaagaaaa
    12301 tgcaatcatg agtctaacaa caatggacaa gattgaaaaa gtgatgattg tctgtgaccc
    12361 tggtatggta gaattcggtt atacaaaaac agttgagaat gtattaagac aaagaacgga
    12421 acagcctcaa attaaaatat ttagcgaagt cgaaccgaac ccatcaacta atacagtata
    12481 taaaggtctg gaaatgatgg ttgatttcca accggataca atcattgcac ttggtggtgg
    12541 ttcagcgatg gatgctgcaa aagcaatgtg gatgttcttt gaacaccctg agacatcatt
    12601 cttcggtgct aaacaaaagt tcctagacat cggtaaacgt acttataaaa taggcatgcc
    12661 tgaaaatgcg acgttcattt gtatccctac gacatcaggt acaggttcag aagtaacacc
    12721 atttgcagtt atcacagata gtgaaacaaa tgtaaaatat ccgttggctg attttgcttt
    12781 aacacctgac gttgcaatta ttgaccctca atttgtgatg agtgtgccaa aaagcgttac
    12841 agcagataca ggaatggatg tactaacgca tgcaatggaa tcatatgtat ctgtaatggc
    12901 ttcagactac acaagaggtt tgagtctaca agcgattaaa ttgacgttcg aatatttaaa
    12961 atcatctgtt gaaaagggtg ataaagtttc aagagagaaa atgcataacg catcaacttt
    13021 ggctggtatg gcatttgcaa atgcattctt aggtattgca cactcaattg cacataaaat
    13081 tggtggcgaa tatggtattc cgcatggtag agcgaatgcg atattactac cgcatattat
    13141 ccgttataat gccaaagacc cgcaaaaaca tgcattattc cctaaatatg agttcttcag
    13201 agcagataca gattatgcag atattgccaa attcttagga ttaaaaggta atacgacaga
    13261 agcacttgta gaatcattag ctaaagctgt ctacgaatta ggtcaatcag tcggaattga
    13321 aatgaatttg aaatcacaag gtgtgtctga agaagaatta aatgagtcaa ttgatagaat
    13381 ggcagagctc gcatttgaag atcaatgtac aactgctaat cctaaagaag cactaatcag
    13441 tgaaatcaaa gatatcattc aaacatcata tgattataag caataatcta tctgataata
    13501 atcatctaac tcacctgaaa ttacaaaagt aaaaaatgcc acataaactt taagtcgata
    13561 atcattatac ggttatcggc ttttatttat tgccaaatct tcagagagat acaaactaga
    13621 caatcatttt tttaaataaa gaaaatatta agattgatac tcatttcgca aactattact
    13681 actttagagt ataattattt ttaatttcat ataaatgaaa agtctaaaat aatgcggttt
    13741 aaaagtaatt aattgtttaa acgatatgta atatgtaaat actatatata taataccaat
    13801 tttaatgaaa atttttaagg gaggtaaata atggaaagta cattagaatt aacaaaaatt
    13861 aaagaagtat tacaaaaaaa cttgaagatt ttaattattt taccgctatt atttttaatt
    13921 attagcgcta ttgttacatt tttcgtctta tcacctaaat atcaagctaa tactcaaatc
    13981 ttagtgaatc aaactaaggg tgacaatcct cagtttatgg cacaagaggt tcaaagtaat
    14041 attcaacttg taaatacgta taaagaaatt gttaaaagtc ctagaatttt agatgaggtg
    14101 tcaaaggact taaatgataa gtattcacca tctaaattgt cgagtatgtt gacaattaca
    14161 aaccaagaaa atacgcaact tatcaacatc caagttaaaa gtggtcataa acaagattcg
    14221 gaaaaaattg cgaatagctt cgctaaagtt acaagtaaac aaattccgaa gattatgagt
    14281 gtggataacg tatcaatttt atctaaagca gacggtacag cagttaaagt cgcaccaaaa
    14341 actgtagtga atctaatcgg tgcattcttt ttaggattag ttgtcgcgct tatatatatc
    14401 ttcttcaaag taattttcga taagcgaatt aaagatgaag aagatgtaga gaaagaatta
    14461 ggattgcctg tattgggttc aattcaaaaa tttaattaag gatggttgct acttatgtca
    14521 aaaaaggaaa atacgacaac aacactattt gtgtatgaaa aaccaaaatc aacaattagt
    14581 gaaaagtttc gaggtatacg ttcaaacatc atgttttcaa aagcaaatgg tgaagtaaag
    14641 cgcttattgg ttacttctga aaagcctggt gcaggtaaaa gtacagttgt atcgaatgta
    14701 gcgattactt atgcacaagc aggctataag acattagtta ttgatggcga tatgcgtaag
    14761 ccaacacaaa actatatttt taatgagcaa aataataatg gactatcaag cttaatcatt
    14821 ggtcgaacga ctatgtcaga agcaattacg tcgacagaaa ttgaaaattt agatttgcta
    14881 acagctggcc ctgtacctcc aaatccatct gagttaattg ggtctgaaag gttcaaagaa
    14941 ttagttgatc tgtttaataa acgttacgac attattattg tcgatacacc gccagttaat
    15001 actgtgactg atgcacaact atatgcgcgt gctattaaag atagtctgtt agtaattgat
    15061 agtgaaaaaa atgataaaaa tgaagttaaa aaagcaaaag cacttatgga aaaagcaggc
    15121 agtaacattc taggtgtcat tttgaacaag acaaaggtcg ataaatcttc tagttattat
    15181 cactattatg gagatgaata agtatgattg atattcataa ccatatattg cctaatatcg
    15241 atgacggtcc gacaaatgaa acagagatgc tggatctttt aaaacaagcg acaacacaag
    15301 gtgtttcaga aatcattgta acatcacatc acttacatcc tcgatatacc acacctatag
    15361 aaaaagtgaa atcatgttta aaccatattg aaagcttaga ggaagtacaa gcactaaatc
    15421 taaagtttta ttatggtcag gaaataagaa ttaccgatca aattcttaat gatattgatc
    15481 gaaaagttat taccggtatt aatgattcac gctatttact aatagaattt ccatcaaatg
    15541 aagttccaca ctatactgat caattatttt tcgaattaca gagtaaaggc tttgtaccga
    15601 ttattgcaca tccagagcgg aataaagcaa taagtcaaaa ccttgacata ctatacgatt
    15661 taattaacaa aggtgcttta agtcaagtga caacggcgtc attagcgggt atttccggta
    15721 aaaaaattag aaaattagca attcaaatga ttgaaaacaa tctgacacat ttcatcggtt
    15781 cagatgcgca taacacagaa atcagaccgt tcttaatgaa agacttattt aatgataaga
    15841 aattacgtga ttattatgaa gatatgaacg gatttattag taatgcgaag ttagttgttg
    15901 atgataaaaa aattcctaaa cgaatgccac aacaagatta taaacagaaa agatggtttg
    15961 ggttataaac agcaaatgag gggttttatg gcacatttat ctgtgaaatt gcggctttta
    16021 atactagcat taatcgattc actgatagtg acattttcag tattcgtaag ttattacatt
    16081 ttagaaccgt atttcaaaac atattctgtc aaattattaa tattggcagc tatatcacta
    16141 ttcatatcgc atcatatttc agcatttatt tttaatatgt atcatcgagc gtgggaatat
    16201 gccagtgtga gtgaattgat tttaattgtt aaagctgtga cgacatctat cgttattacg
    16261 atggtggtcg tgacaattgt tacaggcaat agaccgtttt ttagattgta tttaattact
    16321 tggatgatgc acttgatttt aataggtggc tcaaggttat tttggcgtat ttatcggaaa
    16381 taccttggag gtaagtcatt taataagaag ccaactttag ttgttggtgc tggtcaagca
    16441 ggttcaatgc tgattagaca aatgttgaaa agtgacgaaa tgaaacttga accggtatta
    16501 gcagtcgatg atgacgaaca taaacgcaat atcacaatta ctgagggtgt aaaagtccaa
    16561 ggtaaaattg cggatattcc agaactagtg aggaaatata agattaaaaa aatcatcatt
    16621 gcaattccaa ctattggtca agagcgtttg aaagaaatta ataatatttg ccatatggat
    16681 ggcgttgagt tattgaaaat gccaaatata gaagacgtca tgtctggtga gttagaagtg
    16741 aatcaactga aaaaagttga agtagaagat ttactaggca gagatcctgt tgaattagat
    16801 atggatatga tatcaaatga attgacgaat aaaactattt tagttacggg tgcaggtggt
    16861 tcaataggat cagaaatttg tagacaagtt tgtaatttct atccagaacg tattattcta
    16921 cttggccatg gtgaaaacag tatttattta atcaatcgtg aattgcgaaa tcgcttcgga
    16981 aaaaatgttg atatcgttcc tattatagcg gatgtgcaaa atagagcgcg tatgtttgaa
    17041 attatggaaa cgtataaacc atacgcagtt tatcatgcag cagcacacaa gcacgtgccg
    17101 ttaatggaag acaaccctga agaagcagta cataataata ttttaggtac gaaaaatact
    17161 gctgaagctg ctaaaaatgc agaggtaaag aaattcgtta tgatttctac ggataaagcc
    17221 gttaatccgc ctaatgtcat gggcgcttca aagcgaattg cagaaatgat tattcaaagt
    17281 ttaaatgatg aaacgcatcg aacaaatttt gttgcagtga gatttggtaa tgtacttgga
    17341 tcgagagggt ctgtgattcc acttttcaaa agtcaaattg aagaaggtgg gccagttact
    17401 gtgacacatc ctgaaatgac acgttacttt atgacaattc ctgaagcttc tagactagtt
    17461 ttgcaggcag gggcattagc agaaggtggc gaagtatttg tgctagatat gggagaacca
    17521 gtgaaaattg tagatttggc acgtaattta attaagctaa gtggtaaaaa agaagacgac
    17581 atacgcatta cttatacagg gattagaccc ggcgaaaaaa tgtttgaaga gcttatgaat
    17641 aaagatgaag ttcatcctga acaagtattt gaaaaaattt atcgtggcaa agtacaacat
    17701 atgaaatgta atgaagttga agccattatt caagacatcg tcaatgactt tagtaaagaa
    17761 aaaattatta actatgccaa tggcaaaaag ggagataatt atgttcgatg acaaaatttt
    17821 attaattact gggggcacag gatcattcgg taatgctgtt atgaaacggt ttttagattc
    17881 taatattaaa gaaattcgta ttttttcacg cgatgagaaa aaacaagatg acattcgaaa
    17941 aaaatataat aattcaaaat taaagttcta cattggtgat gtgcgtgata gtcaaagtgt
    18001 agaaacagca atgcgagatg ttgattacgt attccatgca gcagctttaa aacaagtgcc
    18061 gtcatgtgaa ttctttccag ttgaggcagt gaagacaaat attattggta cagaaaatgt
    18121 cttacaaagt gctattcatc aaaatgttaa aaaagtcata tgtttatcta cagataaggc
    18181 agcgtatcct attaatgcta tgggtatttc aaaagcaatg atggaaaaag tattcgtagc
    18241 caaatcaaga aatattcgta gtgaacaaac gcttatttgt ggtacaagat acggtaatgt
    18301 gatggcttca agaggatcag taataccttt gtttatcgac aaaatcaaag caggagaacc
    18361 tttaacgatt acagatcctg atatgacaag atttttaatg agcttagaag atgcggtaga
    18421 actagttgtt catgcattta agcatgcaga gacaggagat attatggttc aaaaagcacc
    18481 aagctcaacg gtaggggatc ttgcgactgc attattagaa ttgtttgaag ctgataatgc
    18541 aattgaaatc attggtacgc gacatggaga gaaaaaagca gaaacattgt tgacgagaga
    18601 agaatacgca caatgtgaag atatgggtga ttattttaga gtgccggcag actccagaga
    18661 tttaaattat agtaattatg ttgaaaccgg taacgaaaag attacgcaat cttatgaata
    18721 taactccgat aatacacata ttttaacggt ggaagagata aaagaaaaac ttttaacact
    18781 agaatatgtt agaaacgaat tgaatgatta taaagcttca atgagatagg agagattgac
    18841 gttgaatatt gtaattacag gagcaaaagg ttttgtagga aaaaacttga aagcagattt
    18901 aacatcaacg acagatcatc atattttcga agtacatcga caaactaaag aggaagaatt
    18961 agagtcagcg ttgttgaaag cagactttgt cgtgcattta gcaggtgtta atcgacctga
    19021 acacgacaaa gaattcagct taggaaacgt gagttattta gatcatgtac ttgatatatt
    19081 aactagaaat aagaaaaagc cggcgatatt attatcgtct tcaatacaag caacacaaga
    19141 taatccttat ggtgagagta agttgcaagg tgaacagcta ttaagacaat atgccgaaga
    19201 gtatggcaat acggtttata tttatcgctg gccaaattta ttcggcaagt ggtgtaagcc
    19261 gaattataac tcagtgatag caacattttg ttacaaaatt gcacgtaacg aagagattca
    19321 agttaatgat cggaatgttg aactaacgct aaactacgtg gatgatatcg tcgctgaaat
    19381 aaaacgtgct attgaaggaa ctccaacgat tgaaaatggt gtacctacag taccaaacgt
    19441 atttaaagtg acattgggag aaattgtaga tttattatac aagttcaaac agtcacgtct
    19501 cgatcgaaca ttgccgaaat tagataactt gtttgaaaaa gatttgtata gtacgtattt
    19561 aagctatcta cctagtacag actttagtta tcccttactt atgaatgtgg atgatagggg
    19621 ttcttttaca gaatttataa aaacaccgga tcgtggtcaa gtttctgtaa atatttctaa
    19681 accaggtatt actaaaggta atcactggca ccatactaaa aacgaaaaat ttctagtcgt
    19741 atcaggtaaa ggggtaattc gttttagaca tgttaatgat gatgaaatca ttgaatatta
    19801 cgtttctggc gataaattag aagttgtaga cataccagta ggatacacac ataatattga
    19861 aaatttaggc gacacagata tggtaactat tatgtgggtg aatgaaatgt ttgatccaaa
    19921 tcagccagat acgtatttct tggaggtata gcgcatggaa aaactgaaat taatgacaat
    19981 agttggtaca aggcctgaaa tcattcgttt atcatcaacg attaaagcat gtgatcaata
    20041 ttttaatcag atattagtac acactggtca aaattatgat tatacattga atcaaatttt
    20101 ctttgatgat ttggaattaa gacaaccgga ccactactta gaggcagttg gaagtaacct
    20161 tggagaaacg atggggaata ttattgcgaa gacatatgac gttttattac gcgaacaacc
    20221 agatgcactt ttaattcttg gtgatacaaa tagttgttta gcagcagtat ctgctaaacg
    20281 attaaagatt cctgttttcc acatggaagc gggtaataga tgctttgatc agaatgtacc
    20341 tgaagaaatc aatcgtaaaa ttgttgacca tgtcagtgat gtgaatctac cttatacgga
    20401 acatagcaga cgttatttat tagatgaagg cttcaataaa gcaaatatct ttgtgacagg
    20461 atcaccgatg acagaagtga tagaagcgca tcgagataaa attaatcaca gtgacgtttt
    20521 aaataaacta ggattagaac cgcaacaata cattttagta tctgcgcata gagaagagaa
    20581 tatcgataat gaaaagaatt ttaaatcatt aatgaatgcg ataaatgata ttgccaaaaa
    20641 gtataaaatg cctgtgattt attcaacgca tccaagaagt tggaagaaaa ttgaagaaag
    20701 taaatttgaa tttgatccat tagttaaaca gttaaagcca tttggtttct ttgattataa
    20761 tgcattgcaa aaagatgcat ttgttgtgct atcagatagt ggaacattgt cagaagagtc
    20821 gtctattttg aagttccctg gtgtccttat tcgaacttcc acagaaagac cggaagtact
    20881 agataaaggt acggttattg taggtggtat tacctataac aatctaatcc aatccgttga
    20941 actagcaaga gagatgcaaa aaaataacga accgatgatt gatgctattg attataaaga
    21001 cactaacgtt tcgacaaagg tagttaaaat tattcaaagt tataaagata ttatcaatcg
    21061 aaatacttgg aggaaatgac gatgaggata gcgattcttg gcgctactaa cattaagcat
    21121 atgtcattat tatcacatta tttaaaccac attgatttga atatcaatga ggtggacatt
    21181 atatacactg acaaatatga tatcgaagaa catatccaag gcatcaataa ttactataaa
    21241 tataaagtag atattaaaga agattggaca tttatcaaaa aagctattgc ttactatcga
    21301 tttaggccat acgctatgaa aattcttaaa gaaaatcgtt atgattttgt catagtatgg
    21361 ggaagttata caggacactt atttaaaagt tttttagaaa aacactataa aaataaattc
    21421 attttaaata taagggacta cttttttgaa aataataaac ttattaagta tagaatgaaa
    21481 aaaatcgttg atgctagcag ggtgacaaca ttatcttcag aaggttttct taaattttta
    21541 cctaaatctg aaaaatatag aattatttat agttataaca tgagtattat tagagaaagt
    21601 aatgtaaccg atgggtttaa aaaaagatgg ccaattaata taggttttat tggtaatgtt
    21661 agatttaatg agataaatca aaaattgata aaggaactgg caaatgattc aaggttccat
    21721 atgcaatatt ttggaacagg gtcggaaaaa ttagaagtct ttgctcgaga aaattttatt
    21781 aataatatta cattttctgg cggctttgac ttgaaagaaa caccgaaata tttaaatgaa
    21841 attgatatac ttaataattt gtttggtaat caaaatattg ctttagatac tgctttatcc
    21901 ataagaatgt attatgcgtt gtttttaaac aaacctatta ttacaacaga cgatacattc
    21961 accgctacag aagctaataa attcggactg ggttttagta ttaatccaga aaatttaaaa
    22021 ggtattggtg atgaattgat ggattggtat aacaatttgg atgtaatgga cattaatcat
    22081 aaaagagaag cttatagaaa cgatgtaatt gaaaataata aacagttcta tcaagagata
    22141 ggcaggatat ttaatgaata agatatataa tgtcacttca tatgttattg ccattttaat
    22201 gtttccttgc cttatgttag gtgataaacc tttattattt ttagcaccta taagttatgg
    22261 agtaggaaag ctctttataa gcttctcgta taatccgaat tttaaatttt cgaaaattgt
    22321 atacgatgtt ttaggttttc ttagattagt atttatacct gctatgatag tgtttttcca
    22381 ggattcaact atagataatt taccattagg acaagcttat tttaatcaag cggttattta
    22441 tatgagtgtg gagtttatca taggctcgct atttatattg atactatcta aattattcaa
    22501 acatgaagtg gtatcaagaa atagctttac actttctgga tcatcaattt attacattgt
    22561 gtttggtctt gttatttgtg ggatttttgt agcttttccc gaagtgcgca aaaacatatc
    22621 atttttaatt attaaaacag atgcaatggg aagaggaacc gaagcaacaa gtggtttaaa
    22681 tgttcttttt gtaatgctat ttcaacttgc cttagcgtta ttattcttaa taatcgcata
    22741 tgcttcatat aaaaagtata aagagaatcc taaaattatt tatgttgtat taccgctagc
    22801 tataggaatt ttaaatatta gtttaattgt tggtgaaaga agaagttatc aactttatac
    22861 aatggttgct gttttaacag tggtgtcaat cttgttttct aagcataaaa gacgtatcaa
    22921 tatcattatt atatctgtag gtattttcgt attagcattg atgacactat ataaagaatt
    22981 gtatgtgttt aattatagtt catatagcga ggcgttaaat agtacaagtg taagtaatct
    23041 taaaatagtt gacacgttgc agtcatattt ttatggacct agcaatattg cagcttctat
    23101 agactatttg aattactata atggttcgtt caaacaatat ttatttgata ataccagagc
    23161 tgtttttggg tttaatttct ttttagataa aaagcagttg attactagcc aactttttaa
    23221 ccaattgata tatggtagta aacaattgac tggtcatcta atttcgagtg ctggatatgg
    23281 aattatttat tttggaccct tattcttcta tttgaattta attgcaaata tcttttttgc
    23341 atttctgagt gagtatatta ttcggaagag tcattcgtta gaagtgatat ttattggtac
    23401 atatatttat atgagactta ttacgagtat ttttagtcac ccgacaccat taatcacatt
    23461 gatttctatg attttagttg tatatgttat agctattatc ccaggcatta taataaaaaa
    23521 atttactaaa aaagtaggga tagaatgatt gtaaaaacat ttatgaaatc gaaaatattt
    23581 agattaatga atacaccact attattattt tataagaaag aatatttaac tggatattat
    23641 tttgaaaata aagtggctgg atggttatgg gcgtggaaag ctgttccgtt caagttgtta
    23701 ggaataaata tgagtttgcc atttcctgca gatataactg ttagaatgca taaccctaat
    23761 aacattgttt ttgataaaaa tgatattcat atttttcaat cgcccgggac gtattttaat
    23821 aatttttcag cagttatata tataggtaga ggtgtttata tagcgcctaa cgtaggtatt
    23881 attacagcta atcataatat taaaaatttg aagtcacatg caccaggtga agatgtcaaa
    23941 ataggaaatt atagttggat tggaatgaac tcagttatat taccaggagt agaattgggg
    24001 gaacatacaa ttgtaggggc tgggtcggtt gtaacaaaaa gttttccaga aggtaacgtt
    24061 gttataggtg gcaatccagc gaaaatcatc aagaaaatct gaggttaatt atgagattaa
    24121 ataaatttat tggcgattcg ttcttaatga ttttaagcag tggcatcgct caagtcatat
    24181 taatcatcac taccccaatt attacaagac tatattcacc tacagaattt ggtgagttta
    24241 caattttttc aaatatcgca atgattttaa taccaataat aaatgcaaga tacgatttgt
    24301 tgattgtgaa tactaaaaat gaccgtagtg ctaatatact ttcacaaatc agttttttga
    24361 tatcattgct tattttatta atactgatac caatatttgc gattagtgca tgtttatacc
    24421 caa
//
LOCUS       DAHHYJ010000025        23507 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000025 DAHHYJ010000000
VERSION     DAHHYJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23507
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.025"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(194..607)
                     /locus_tag="NBK22_000760"
     CDS             complement(194..607)
                     /locus_tag="NBK22_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413963.1"
                     /translation="MKPTKVILKDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(636..746)
                     /locus_tag="NBK22_000761"
     CDS             complement(636..746)
                     /locus_tag="NBK22_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413964.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            complement(832..1563)
                     /locus_tag="NBK22_000762"
     CDS             complement(832..1563)
                     /locus_tag="NBK22_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD2413965.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHNFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            complement(1565..2878)
                     /gene="murT"
                     /locus_tag="NBK22_000763"
     CDS             complement(1565..2878)
                     /gene="murT"
                     /locus_tag="NBK22_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCD2413966.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYTVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            3170..3670
                     /gene="ftnA"
                     /locus_tag="NBK22_000764"
     CDS             3170..3670
                     /gene="ftnA"
                     /locus_tag="NBK22_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCD2413967.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
     gene            complement(3752..3844)
                     /locus_tag="NBK22_000765"
     CDS             complement(3752..3844)
                     /locus_tag="NBK22_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413968.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            4060..4614
                     /locus_tag="NBK22_000766"
     CDS             4060..4614
                     /locus_tag="NBK22_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCD2413969.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            complement(4681..5751)
                     /gene="dinB"
                     /locus_tag="NBK22_000767"
     CDS             complement(4681..5751)
                     /gene="dinB"
                     /locus_tag="NBK22_000767"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCD2413970.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGMTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(5998..6528)
                     /locus_tag="NBK22_000768"
     CDS             complement(5998..6528)
                     /locus_tag="NBK22_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCD2413971.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLILLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            complement(6698..8059)
                     /gene="rlmD"
                     /locus_tag="NBK22_000769"
     CDS             complement(6698..8059)
                     /gene="rlmD"
                     /locus_tag="NBK22_000769"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCD2413972.1"
                     /translation="MQAIAKNDIKTGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKINDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWSQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKSDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            complement(8140..9087)
                     /locus_tag="NBK22_000770"
     CDS             complement(8140..9087)
                     /locus_tag="NBK22_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD2413973.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMALASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            complement(9612..9758)
                     /locus_tag="NBK22_000771"
     CDS             complement(9612..9758)
                     /locus_tag="NBK22_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413974.1"
                     /translation="MIADLDAIEGLFISIEVILLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            complement(9798..11225)
                     /gene="gatB"
                     /locus_tag="NBK22_000772"
     CDS             complement(9798..11225)
                     /gene="gatB"
                     /locus_tag="NBK22_000772"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCD2413975.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            complement(11238..12695)
                     /gene="gatA"
                     /locus_tag="NBK22_000773"
     CDS             complement(11238..12695)
                     /gene="gatA"
                     /locus_tag="NBK22_000773"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCD2413976.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHKENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADVNDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KKAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            complement(12697..12999)
                     /gene="gatC"
                     /locus_tag="NBK22_000774"
     CDS             complement(12697..12999)
                     /gene="gatC"
                     /locus_tag="NBK22_000774"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCD2413977.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            13365..14903
                     /gene="putP"
                     /locus_tag="NBK22_000775"
     CDS             13365..14903
                     /gene="putP"
                     /locus_tag="NBK22_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCD2413978.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            complement(14992..16191)
                     /locus_tag="NBK22_000776"
     CDS             complement(14992..16191)
                     /locus_tag="NBK22_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCD2413979.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKKSQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKGKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKIKDDKEP
                     QVHIYSN"
     gene            complement(16204..18207)
                     /gene="ligA"
                     /locus_tag="NBK22_000777"
     CDS             complement(16204..18207)
                     /gene="ligA"
                     /locus_tag="NBK22_000777"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCD2413980.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNVDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            complement(18211..20403)
                     /gene="pcrA"
                     /locus_tag="NBK22_000778"
     CDS             complement(18211..20403)
                     /gene="pcrA"
                     /locus_tag="NBK22_000778"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCD2413981.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            complement(20400..21092)
                     /locus_tag="NBK22_000779"
     CDS             complement(20400..21092)
                     /locus_tag="NBK22_000779"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCD2413982.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVVFHNGTLLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            complement(21264..21566)
                     /locus_tag="NBK22_000780"
     CDS             complement(21264..21566)
                     /locus_tag="NBK22_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413983.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            complement(21675..22970)
                     /gene="purB"
                     /locus_tag="NBK22_000781"
     CDS             complement(21675..22970)
                     /gene="purB"
                     /locus_tag="NBK22_000781"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCD2413984.1"
                     /translation="MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFERAGLA"
BASE COUNT         7157 a         4327 c         3325 g         8698 t
ORIGIN      
        1 gtcaactact gccaatataa cttcgtagag catagaacat tgatttatgt ccccgcctct
       61 ttttatcgta tatagcatgt ccctttttaa aattacaagc ataaatattc agactcaaat
      121 atagccacac gcctaaacta cggagggatg tggctgtctt ttttgattgt aattatctat
      181 cagatttaaa tcattaatgt aaagaggcta attcattaat aaatttagta aaatctgatt
      241 tagcaacatt tatccttaat gaaataccag attctgttgc catgtttgaa tgccttaaac
      301 cagaatcaaa atcaatataa atacaaagca gttcttgatt aaaatcaata attgtaaaat
      361 gtaaatcagg ttctataaaa tatatttcag taacaggatt gtcgtaatcc atatgtttca
      421 aattgtccat gtcactatca aaaagtgcaa attcaaattt aacttcttga tcttcgaaag
      481 ttgcactagt gtcgaaataa taaattttat tatcttcttc gataactaca tcttttaaaa
      541 taaatgttat cgatgttttg ctatgtaaat aagatgcatc ttttaatatc actttagtag
      601 gcttcatcgt caaattcctt tcatcattca ttttttcatg ttgcataatc accattacta
      661 ttgataattc taggatagat actcattgta taataattat tcactagaac aaaaaaacac
      721 acatcaattt ggttgtttcc atccaaaata atgtgtgtgt ttatacatat atgatttatt
      781 actaaacttc atcgtttatc aatattaaat ccattgatgc gattttcaga gttaacgaga
      841 tttcttctgt ctatttgctc tgtctattaa tacttgtttc gcttgtattt ccgcttcatt
      901 atctatttct ttaggctcaa acggaatccc cttacgttca caagcttttt ctaacagata
      961 atcagtgatt tcgtaatttt taggtaaaat tggtccgtgt aaataagtac ctaataaatt
     1021 tttataatga atgccttctt ttttatcttc atcattatta ccataaccaa aagtaacatg
     1081 accaagtgta ccgaaattat gatatgttct accaccgtga ttttcaaaac ctacaatagt
     1141 tccaaaagta tcactttcga taacaatatc tcctgttaat cggtttgtct ttgattcagt
     1201 ataaaaatct aaaataccta acccttctaa ttctgtacca tcaggcgtga tatatttttt
     1261 tcctaaaaat tgatagcctc cacaaattgt taatcccggc ataccatctt caatcgcttc
     1321 tttaagtggt gtcttaattt tacttaattc ttttgttgct aatgcttgtt ctctatcact
     1381 tccaccaccg ataaagaaaa tatcacattc atcaaaggta ataccttctg tttcattgat
     1441 ttctacgaca ttaactttaa tatttcgttt tttagcacgt tgtcttaaag caataatatt
     1501 tcctatatca ctgtataaat tcaatttatc tgacataaaa tgataaatag tcaattcatg
     1561 catattatga ttgacctcct tcaaacgaac ggtttaattg ttcaagcata ggcgctaatg
     1621 atgtatagtt tggtattgca actgtgaaac ctttataatc catagtcttt gccgttgctt
     1681 tataaatatc acgctcaact ataattggta cttcaacctc tgctaacttc aatcgcaatt
     1741 gaagttcttc tgctcgtgta cctgtcacga tgatagcttc aatttgttgc ttagataatt
     1801 tttcaaaatc tgcatcataa atccatgaag tatctcgacc atctgcagcg ttatcattta
     1861 gcgaaataac atacactttt tcgccttcta attgttcacc aactgataaa cttgcattca
     1921 ttcctgcagg atttttagct aaattgatca tcgcttcttt tcgttctttt ttaaagtact
     1981 gcatacgacc attgtctgat gtatacgttt caaagccatt tttaattgtt tgttcattta
     2041 accctagctc tcttaaaaca gtatatgctg ctaacgcgtt ataagcgtta aagtcacctg
     2101 caattttcat atcatatttt tcatcattga tatttaaatg taaaaacggt gccacatcaa
     2161 aacttgatat ttcatatttt gcttgctctc gtttgaaacc acactgacag tgataatgac
     2221 caatttgatt ataatgaata taatcgtatt gcaataagcg accacagttt ggacaatatc
     2281 tactttcatt catcgtactt tgttcaaatt catgggcatg tgctttcata ccatagtaca
     2341 caatcgtatc acttgcgatt ttcaaacgac tcacaaatgg atcatcagca tttagcaata
     2401 atttgatgcc tttattacta attgtctctg caatgttatt aaccataata tcaatttcac
     2461 cgaagcgatc catttgatct ctaaagaaat tagtaaatac catcattgaa ggtgtaactt
     2521 cttttaacac acgtggaatc gaaccttcat caatttcgat taccgcaatt ttagtcttag
     2581 gtgttgattg catgatgaat gcagaagtta tacctgcagc catattagca ccttcattat
     2641 tgtgtataat ttgaatatta tttgctttta aagtatgtcc aattaagttt gaagtcgttg
     2701 ttttaccatt tgttccactg ataaatacaa tatcatcaac ttgctctgct aattttctta
     2761 atatatctgt atccactttt ctagcgattt gtccaggtaa atctgttcct cttttaccta
     2821 ctgctctact tgctttacgc gccaatttcg ctagatggat tgccgtccac tgtctcatgt
     2881 gtttcctcct caaatttcca ctcgcatcat tataacatga caaggcaact tcaaaaaagt
     2941 ttctcaatca caaattgata cagtgtttct aaattataat cattatcatt tagataatta
     3001 aaaatgatat ttattctcaa ttaaatatcg ctaaatgata tattttctca attagagtgc
     3061 taaaataagg cattaaatta taattattat tatttacaat tgagaatgag tttgttatac
     3121 taagtttaat tcatagtaat tttaatttac aaataagagg tgtatcaaaa tgttaagtaa
     3181 aaatttatta gaagcattaa atgatcaaat gaaccacgag tactttgcag cacacgcata
     3241 tatggcaatg gcagcatact gtgataaaga atcgtacgaa ggatttgcaa acttcttcat
     3301 tcaacaagct aaagaagaac gtttccatgg acaaaagatt tataactata ttaacgacag
     3361 aggtgcacat gcagaattca gagcagtttc agcaccaaaa attgactttt caagcatact
     3421 agaaactttc aaagacagct tatctcaaga acaagaagta acaagacgtt tctataactt
     3481 atctgaaatc gctcgtcaag ataaagatta tgcaactatc tcattcttaa actggttctt
     3541 agatgaacaa gtcgaagaag aatcaatgtt tgaaacgcac atcaattatt taactcgtat
     3601 cggcgatgac agcaatgcat tatatcttta cgaaaaagaa cttggcgctc gtacattcga
     3661 cgaagaataa ttaaacatca ctacaataga cagataaata tcatacgaca tgataggcat
     3721 ttgggtcact tacaataacc caatgtctat attattttgc tttacggaga tcactagatt
     3781 cattttctga atcattgatc tgcgtttttt cattttcaag gctaattatt gtatttttag
     3841 tcatttattt tttaaactac taatgttaat aactctaaat ttgatgttga attaatttga
     3901 ccgattttaa agcatatcat catttacttt ttaatcagag ttacatccaa atgatagatt
     3961 tcacgttata ccttcacgta taatattatg tatcgtttgt aagcaaatga ctaaaagtct
     4021 attaatatat atatttaatt aattgaaagg attgactaca tgatacaaga tgcgtttgtt
     4081 gcacttgatt ttgaaacagc aaatggtaaa cgtacaagta tttgttctgt cggaatggtt
     4141 aaagtcattg atagtcaaat aacagaaaca tttcatactc ttgtgaatcc gcaagactat
     4201 ttttcacaac aaaatattaa agttcatggc atacaaccag aagatgttga aaatgcacct
     4261 acgtttgact acgtatttcc atatatgatg caatttattg cagatttacc tgttgtcgca
     4321 cataacgcgg catttgatat gaacgtctta catcaaagca ttcaaaatat tggtttacca
     4381 actccaaatt taacttactt ttgtagttat caacttgcta aaagaaccgt tgattcgtat
     4441 cgatacggtt taaaacatat gatggagttt tatcaattag attttcatgg tcatcatgat
     4501 gcattgaatg atgccaaagc atgcgcaatg attactttta ggctactgaa aaattatgaa
     4561 aatttaacat atgtaactaa tatttatggt aaaaatctaa aagataaagg ctaggactaa
     4621 ataaaatact cccttcaaag taagcattgt aaaaatgtaa actttgcagg gagctttatt
     4681 ttatataaag tcatatatcg tcatattttt ataagttgat tgttctaaat tacctacagt
     4741 gacaccaata agtcgaattg gtacatcagg gtcttttaaa tcgttataaa gtaaatatgc
     4801 aatattataa atatcttctt cagaactaac cgaatctctt aaactcatct gtttagatag
     4861 cgtttcaaat tgataagttt taattttaac cgttacagtt ttagctgact tctgtaattt
     4921 atttagacgt tcagctgttt tacctgacaa ttcccatact tttcttaaaa tctcttcatc
     4981 atcattcacg tctgttgcaa atgtgcgttc agtccctact gattttctta ctcttgatga
     5041 tttcacttca ctatggtcaa taccgcgtgc cttgttatat aaaccccgac ctctttttcc
     5101 aaacaaacgt attaattcaa attccgtttt ctcatataaa tctctaccgt taaaaatacc
     5161 attatcatgc attacttttt tggaagcttt acctacgcct ggaaaatctc caatatccaa
     5221 tgtcatcaaa atatcatgga cattttgata atcaatcaca gtcataccat caggtttatt
     5281 cataccactc gctaatttag ctaaaaattt gttataagaa acacctgcag atgctgttaa
     5341 atgtgtctgc tctagaatat cttttctaat atactgagca attttcgaag caggaaggtc
     5401 tggtctcact aattctgtaa tatctaaata cgcttcatcc aatgacatcg gttctacctt
     5461 atctgtataa cttcggaaaa tagacataat ctgcgcagat gtttctcggt aagcaccaaa
     5521 attacttgtg acaaagtatc catttggaca taatttatgc gcttgtgaca taggcattgc
     5581 tgaatggacg ccgtattttc gtgcttcata agatgccgta gagacaacac ccctactgct
     5641 tgctttacca ccaacaatga ctggtttccc tttcaatttg gggttatctc tcatttcgac
     5701 ttgtgcaaaa aaatagtcca tatctatatg aataattcgt ctctcagtca agtgctcacc
     5761 tccctactaa tttttacttt tataacacac aaaaatatct caacataatt atacgctgtg
     5821 tacgattttt ttacataaat cttgcactta gcgataacta tattgagata actacaagtt
     5881 gttataaaat caattgctat ttaagcatga tgatgaagac gattgagtaa gaaaacatag
     5941 gtaatctgaa ataattcaag caaattcatt ttgttggtat tatcatatta aaatttatta
     6001 ttgagtcggc ttttgatgat acaaataaat actatcttca tcttgttgaa catatttaaa
     6061 actactagat attgttaatg caacaagcag aatatctata aagcaataag cggtatgcat
     6121 actaaacata aaaataagtg atgaataacc aaatacctta attaaaataa gcaagccagt
     6181 acttaataag attagtggtg acagcataat aattgagaat tgccatttgt tgaagcaagc
     6241 atctgctgtt tggaataaga ttctgtcttt ttttatatta aacataggtt tgctatcttt
     6301 tttaaataaa agaaataatg ctctatggat aagttcatgt aaaatcaata aaataatgaa
     6361 tccagcaaac ccatatacaa gattgatgat gatattttga tcgacaaccg ctgtgacacc
     6421 taacgcccac ttatacgtaa ataaaatcac gaataacgca ataacaagtt gcaagataat
     6481 aaaccttcgc atttgaaaat tatttgtcgt taaatcaatt ttatgcatta ccaaccctcc
     6541 cgatcatgac attcttattc ttctttaaat atagtataca atgtcacatt taatttaaaa
     6601 agttcatatc aagaaagtaa attggctgta ataaaatttt aatatacgac ttctttcttc
     6661 acttattaag gcgaaatttt atctcaaatc atgtgcgcta tttcaaattg aataatgcca
     6721 ctgtctcaac atgtgttgtt tgtggaaaca tatctaccgg tgttacctct tcaagttgat
     6781 atttttcagc taataataat gcatcacgtt gctgtgttgc gggattacat gaaatataga
     6841 caatacgctt aggttctaat gtaagcaaag tctgaataaa cgtttcgtca cagccctttc
     6901 ttggcggatc aaccattaca acatctgatt taatcccttg tgctttccat tgtaaaataa
     6961 cttcttcagc tttcccacag acaaaagttg tattattgca ttggtttata gtcgcatttt
     7021 gttgtgcgtc ttcaattgca gaaggtacta cttcaacacc gtatacatgt tttgcatgtg
     7081 gtgccatata tagccctatt gttccaatac cacaataggt atctaataca acttcattac
     7141 ctgtcaattg cgcatactca attgctttat tatataattt ctctgtttgt tcagaattaa
     7201 tttggtagaa tgactgatca cttattttaa atgtactatc tgttaattga tcaataattg
     7261 tatctttacc atatagcgtt atagattgac gtcccataat aacattagag tggctatcat
     7321 taatgttttg tttaatgctt gtcacattag gaaatgcatc taatatcttc tcaacaacag
     7381 catttttttg tgaccacttt ttaccattag ttacaaaaat aatcatcatt tcgtctgtat
     7441 gatatcctgt tcttacaacc aaatgtctca ttaaaccttt tttcaattgt tcttgataaa
     7501 tacttacatt taaatctttt aaaatagatt taacttcatt catcacttct tgatgttgtg
     7561 aatcttgtat taaacaactt tccatgtcaa taatgtcatg gcttctttga cgataaaagc
     7621 ccataataac ttcattctgt tcattcttac caactggaat ctgggacttg tttcgatatc
     7681 tccaaggatc tgtcatgcca actgtatcgt taatcttaga attatcaaaa tgcgcttttc
     7741 gctgaaacaa attaatcact tgttcctttt tcatttcaag ttgtgcttcg tatgataagt
     7801 gttgaagttg gcacccacca caacgttcat aatatataca tggcggtgca acacgattct
     7861 cacttctcgt atttatatta agcaacttac ctatcgcaaa gttctttttt actttgataa
     7921 ttttatattc aatttgttca ttaattaaag cttgtggtat gaaaatagga aagcgatcta
     7981 tttttacgac accatggcct tcatgcgtta aatcaacaac tgttcccgtt tttatgtcat
     8041 ttttagctat tgcttgcaaa attttacctc caaaatgaac aggttaggaa caaaattatg
     8101 cgcttcctaa cctgccatta tatatttcac tatttctgtt tattcttcga ttaaattgtc
     8161 atcaacatga tcattattta ttaactcttc atttacaata tcattaggtg caaagacatc
     8221 tatatgacgt tctagattta agaaattcgc tggtaattta ccaccatatt ctccatctac
     8281 atttagttgt aagtctgtga atgatgaaat attaattgcc tttgcttttt cataaataac
     8341 tttaggatgc ttagtatgtt ctcctcttga agctaaagcc ataatatgac caagttctgc
     8401 aaggtttgat ttttcaacta taattaacgt aaaatagccg tcatctaact tagcgtccgg
     8461 cactaatttt tcaaatcctg ccattgaatt tgttaaacct aaaaagaata ataatgcttc
     8521 tccttggaaa acattaccat catattcaat tcttaaatct acagctttca tttgaggtaa
     8581 catttcgaaa cctttgatgt aataagcaaa tggaccaaca atagatttca atttactcgg
     8641 tgtttcataa gagacttgcg tcaattgtcc gcctgcagct aaattaataa agtatcgatt
     8701 attcatttta ccaatatcta ctttagtaga atgaccttca atgatgacat caagtgcccc
     8761 catgatgtca ttaggtatat gcaatgcacg tccaaagtca ttaacagtac ccataggaat
     8821 gacacctagc ttaggacgat taggcttttc tgcgatacca ttaactactt catttaatgt
     8881 tccatcacca cctgcagcga ttaatacatc ataattttca tgcatagctc tttctgcttc
     8941 aagtgtggca tcacctattt tctcggttgc atatgcactc gtttcatatc ccgctttttc
     9001 taattttatt aaggcatcag gtaattctct tttaaatagc tctttacctg atgtcgggtt
     9061 ataaatgatt ctagcacgtt tcctcatatc ttatccctct acttaaaatt catatatttt
     9121 aacttcatct ttgtttcgtc taatagggag tgggacagaa ataatattta acaaaattta
     9181 tttcgttcta ccccaacttg cattgtctgt agaatttcct ttcgaaattc tctatgttgg
     9241 ggccccaccc caacttgcac attattgtaa gctgacagaa agtcagcttc tatgttgggg
     9301 ccccactaga attgaaaaaa gcttgttaca agcgtatttt ctttcagtca actactgcca
     9361 atataacatt gtagtgccta ggacattgaa tttatgaccc aggctcagtc ttatttcatc
     9421 attcttaata tcgttaaaga ccaacttgta tcttaaacaa atactatctc aatatgtaca
     9481 aagcttgtta tttattcagc attttttgcc gttcttcatt atatagactt cgtcagttat
     9541 gctattttac ctttaaaatg atgttgtaaa tataatgttg tctttataca aaaatgtttt
     9601 ataccttttt attatacagt ttgccttaat ataattaaag ctaacgcatt aataaaatta
     9661 atatttttac cattaacatg tacaatgaat aaagttaaaa gtaatatgac ttctattgat
     9721 ataaataaac cctcgattgc atctaagtca gcaatcaagg gtttattttt taaatcttca
     9781 tagtttgatg atttaaatta tcttttatct aattcttgtt ttaatagttg atttactaat
     9841 tgtggattag cttgaccttt agacgctttc ataatttgac caactaagaa gcccatagct
     9901 ttgcctttac catttttgta atcttcaact gattgttcgt tattgtctaa tgcttcattt
     9961 acaaatttta gaagtgttgc ttcatcagaa atttgaacta agccattatc ttccataatc
    10021 tgtttagcat taccaccttt agctgctaac tctgggaaga ctttcttcgc aattttactg
    10081 ctcattgttc cgtcttcgat aagtttaatc atacctgcta aattttctgg tgttaattta
    10141 gtatctaata attctacttg atttttattt aaatattcgt ttacgccacc cattaaccag
    10201 ttagatgtta atttaacatc tgcaccgtgt tcaattgttg attcaaagaa atctgacatt
    10261 tctttagtca atgttaatac gtgtgcatcg tatgcaggta aacctaattc atttacatac
    10321 ttagctttac gttcatctgg taattcagga attgtctgac gaacacgctc tttccaagca
    10381 tcatcaatat ataaaggtac aatgtcaggc tctgggaagt aacggtaatc atcagaacct
    10441 tctttaacac gcattaaaat tgttttacct gtagattcat caaatcgacg tgtttcttgt
    10501 ccgatttctc caccatttaa caattcttct tcttggcgtt tttcttcata ttctaaacct
    10561 ttacgtacat agttaaatga gtttaagttt ttcaattcgg ctttagtacc aaatttttct
    10621 tgaccatatg gacgtaaaga gatgttagca tcacaacgta aagatccctc ttccatctta
    10681 acatctgata caccagtgta ttgaataatt gaacgcaatt tttctaaata tgcatatgct
    10741 tctttaggtg aacgaatatc tggttcagat acgatttcaa ttagcggtgt accttgacgg
    10801 ttcaagtcaa ctaatgaata ctcaccttta tgtgttgact taccagcatc ttcttccatg
    10861 tgaagacgag taataccgat tcgttttgtt tcaccgtcga cttcgatatc gatatatcca
    10921 ttttcaccaa ttggttgatc aaattgagaa atttgatatg cttttggatt atctggatag
    10981 aaatagttct tacggtcaaa cttagattct gttgcgattt ccatatttag tgccattgca
    11041 gcacgcattg cccagtctac tgcacgctta ttaacaactg gtaagacacc tggatatgct
    11101 aagtcgataa catttgtatt tgagttaggt tctgctccaa aatgcgctgg tgatggagaa
    11161 aacatttttg agtccgtttt taactctacg tgaacttcaa gtcctataac tgtttcaaaa
    11221 tgcatgattt ccactcctta taatttttca taaacgtcat gtaaattgta ttgtgtttca
    11281 tattgataag cgacacgata taacgttttt tcatcgaatg gtttaccaat gaactgtaaa
    11341 ccgattggtc ggccatttga ttgtccacaa ggaacagaaa taccaggtaa tccagctaag
    11401 tttactggtg ttgttaataa atcattggca tacattgtta atggatcatc aatttcttca
    11461 cctaaattaa acgcagttgt aggcgctgtt ggaccaacta ctacatcata attttcgaat
    11521 actttatcaa agtcattttt aatcaatgtt ctaacttttt gagatttttt atagtaagca
    11581 tcatagtaac ctgaacttaa tgcaaatgta cctaagaaaa tacgacgttt tacttcttta
    11641 ccgaaacctt cagatcttga cattttatat aattcttcta atgaatgagc ttctttagaa
    11701 tgataaccat aacgaattcc gtcaaaacga gaaaggtttg acgaagcttc tgatgatgca
    11761 atcacgtaat atgatggaat accaaattta gtatttggca atgatacttc ctcaacgaca
    11821 gcacctaaag attttaaagt ttctacagcg ttttgaactg ctttttttac gtcatcagct
    11881 acaccttcac ctaagtattc tttaggtaat gcaactttta atcctttaat atctttacca
    11941 atttcagatg taaagtctac atcatcaact ggtgcacttg tagagtcatt aacatctgca
    12001 ccagaaatag cttctaatac gattgcatta tcttttacat ttcgagtcaa tggaccaatt
    12061 tggtctaatg aagatgcaaa agcaactaat ccaaatcgag atacacgacc gtatgttggt
    12121 ttcataccga caacgccaca atatgcagcc ggttgtctaa ttgaaccacc tgtgtctgaa
    12181 cctaagctaa atggtactaa gccagctgca actgctgctg cagatccacc tgatgaacca
    12241 cctggtactg ctttatggtc aaatgggtta actgtttttt tgaaataaga tgtttctgtt
    12301 gaaccaccca ttgcaaactc atccatattt aatttaccga ttaaaacggc attctcttta
    12361 tgtagttttt ccattacagt agattcgtaa attggcacaa aaccttctaa cattttactt
    12421 gcacatgttg tttctaatcc gtttgtaata atgttatctt ttatacccat tggaatacca
    12481 aataatttgc catccatttg atcttttgct tgtaattcat ccaattcttg cgcttttttg
    12541 attgcatttt ctttatccag cgctagaaaa gacttaattg ttggatcagt ctcttcaatt
    12601 gcatcatata tatctttaac aacatcagat ggtttgattt ttttgtcttt tattaaagtt
    12661 aataaattct caaccgattc gtagcgaatg ctcatcttac gcgtcctcct cattcatgat
    12721 tgtaggcact ttaaattgtc catcttctgt ttctttggca tttttcaaag ctaattcttg
    12781 cggaatacct ttaattgctt tatcttcacg taaaacgttt tgtaaatcta aaacgtgata
    12841 tgtaggttca acgccttctg tatcagcgct atcattttgt tttgcaaaat ctaaaatgct
    12901 ttctaatgtg ttggccattt cttccgtttc ttcaggagaa atttgaagtc ttgcaagatt
    12961 cgcgatatgc tcaacttctt cacgtgttac ttttgtcatt aataaaagcc tcctttaagt
    13021 cattcatcac taaattgtat caaatttcca attaaaaatc taagtattta tgaggtgcta
    13081 ctttaatttc atataaactg tataaacatt atcattcgtt tatcaaatca ttttttatga
    13141 aaacaacact cttttaatat tagacaaccc aattcaatat tatgattatg caattttaat
    13201 tataaaaatt tgctcatatc caaaaatacg aaagcgcttt ctatattggt atgcaagtat
    13261 ttcaaaaaga ataaatttaa ttttcctact tttctaaaca tttatcttta tgtataatgt
    13321 tttcaagtaa ctaaattata aattaaataa agggagtgtt tatcatgctt acaatgggga
    13381 cagcattaag tcaacaagta gatgccaatt ggcaaactta tattatgatt gccgtctact
    13441 tcttgatact aatcgttatt ggcttttacg gttacaagca agcaactggt aacctaagcg
    13501 agtacatgtt aggtggacgt agtattggac cgtatattac tgcattatca gctggagctt
    13561 cagatatgag tggatggatg attatggggc tacctggttc tgtctatagc actggtctat
    13621 cagctatgtg gattacaatc ggtttaacat taggtgctta tataaattac tttgttgttg
    13681 ctcctagact tcgtgtttat accgaattag ctggagatgc aattacatta ccagatttct
    13741 ttaaaaatcg tttaaacgat aaaaataatg tgttaaagat tatttctgga ttgattatcg
    13801 tagtattctt tacattatat acacattctg gtttcgtatc tggtggtaaa ttatttgaaa
    13861 gtgcttttgg attagattat catttcggtt taatattagt tgctttcatt gtcattttct
    13921 atactttctt tggtggatat ttagctgtat caattacaga tttcttccaa ggtgtcatta
    13981 tgttaattgc gatggttatg gtccctattg ttgctatgat gaatttaaac ggctggggaa
    14041 cgtttcatga tgtagcagct atgaaaccta caaatttaaa tttatttaaa gggttatcat
    14101 ttataggaat tatctctcta ttttcatggg gattaggtta tttcggtcaa cctcatatca
    14161 ttgtaagatt tatgtctatt aaatcacaca agatgctacc taaagctaga cgtttaggta
    14221 ttagctggat ggctgttggt ttattaggcg ctgtggctgt tggtttaaca ggtattgcat
    14281 tcgtacctgc ttatcatatt aaactagaag atcctgagac attattcatc gtgatgagtc
    14341 aagtgctttt ccatcctctt gtaggtggtt tcttacttgc tgcgattcta gctgcaatta
    14401 tgagcacgat ttcttcacaa ttacttgtaa catctagttc actaacggaa gacttttata
    14461 aattaattcg tggtgaagaa aaagctaaaa cgcaccaaaa agaatttgtt atgattggaa
    14521 gattatctgt attagttgta gcaattgttg ccatcgcgat tgcatggaat ccaaacgaca
    14581 caattctaaa cttagtaggt aacgcttggg ccggatttgg tgcatcgttc agtccacttg
    14641 tgctatttgc actttactgg aaaggtttga cacgtgccgg tgctgtaagt ggaatggttt
    14701 caggtgcctt agtcgttatc gtttggattg cgtggattaa accattggca catatcaacg
    14761 aaatattcgg cttatatgaa attattcctg gatttattgt aagtgtaatc gttacatatg
    14821 ttgtaagtaa acttactaaa aaacctggtg catttgttga aactgactta aacaaagttc
    14881 gtgacatcgt tagagaaaaa taattcataa gtcttaacaa attaaaaagg tactaatgtt
    14941 aatcaaaatt atggctaaca ttggtacctt tttattatct ttaatttaca attaattact
    15001 gtaaatatga acttgcggtt ctttgtcatc ttttatctta ctaattaaag cacgtggttg
    15061 gttaccatct ttaatacgaa tttcatagtt atcgatttta tcgaaatatt tattcgcttg
    15121 ttcagtaacg tactgtgtaa taccaattgt ttcagcttgt ccatagtaat cgattggtaa
    15181 atctactact aatcgttgtg gctttttatc aacaaattta actttcccta ctgcttgtgt
    15241 gaaattagaa aaatatgatt gcaaattatc attaaattgc ttgaaattat tatttaaatt
    15301 ttcatcataa tctgctgctg ttgaagaagg taataaagct gatttttcat tgatattatg
    15361 ccattcatta agctttgttt gactcttttc tgcagtcgct tgagtgataa attcacctgg
    15421 tgtgattgaa tcttcacttg attgcttata aattgcaaaa tgaattggta tatcttttaa
    15481 atcatcattt tcacgtaacc ttgataatat ctcactagcc atttgtttac cttgcttttt
    15541 aacttcgcta tcatctaatt ttttactaaa agtcggtcca tctttttctt ttttatagta
    15601 ataaacacta ttcatagcta aaccaatcgt cataccttta atattcttac cttttgtatc
    15661 tccaccacca taaaaatctt gctctaaaat gttagataaa taggctggtg atttttctgc
    15721 aatcttttca ggatctgttt caccttcgtg tgatggatta agtcctaaat tttcattcgc
    15781 tttcttgtct tttttatctt tttcagacat cttatcgatt tcacgttttg tatacttagg
    15841 atttaaatag gcattaattg ttttcttgtc caaaaattga ccatcttgat acaaatattt
    15901 atctgttgga aatacttctt tacttaagtt caataaacca tcttcaaagt cgccgccatt
    15961 ataactattt gccatgttat cttgtaaaag tcctcttgcc tggctttttt taaatggtaa
    16021 caatgtacga tagttatcac cttgtacatt tttatccgta gcaatttctt ttacttgatt
    16081 tgaactattg ttatgttttt gattatcttt tccagcctgg tcatccttat ggttaccaca
    16141 agcagcgagt ataaagatag ctgtaatcaa taatactaat gtacgcttca tcgacatacc
    16201 cctctaacta tttaattcat tttgcttatc tacaaattgt tgctctgtcc aaatttcaat
    16261 acctaaactt tgtgcttttg ttaattttga acctgcatct tcaccagcaa taacgacatc
    16321 tgtattttta gtaacgctac ttgtaacttt agcaccttgt gatgcaagcc atttagatgc
    16381 ttcattgcgt gtcatttgat gtagcttacc agttagtact atcgttttac cactaaattc
    16441 aggatgtcct tcaatatctg atgttttgat acctttataa atcatattaa catgtttatc
    16501 ttttaatttt tgaattaaag cacgaatatc ttcattttct aaataagtaa ctacagattg
    16561 tgctacttta tcacctatat catgaatttc tactaattcc gcttcagtta ccgttagtaa
    16621 tcgatctatc gtttcatatt tttctgctaa cacttggctc gctttaacac ctaaatgcct
    16681 aatacctaga ccaaataata aattttctaa agagttgtcc ttagcttgtt gaatggcagc
    16741 taataaatta tcaacttttt tctgccccat tctgtctaaa ggtaataaat cttcttctgt
    16801 taaatagaaa atatcagcaa catctttaat taattcgctt tgataaagct gttgaataat
    16861 tttagtgcct aaaccatcaa tattcatggc ttgtcttgat acaaagtgaa ttaatccttc
    16921 aacaagttgt gcttggcatt ttggattaat gcaacgaagt gctacttcgc cttcaatacg
    16981 tactaattca tgtccacaac ttggacaatg ggttggcata tgatatgtga cagcatcctc
    17041 aggtctacgt tctggaatac tacgtacaac ttcaggtatg atgtcacctg cttttttcac
    17101 tacaacacta tcaccaattc gaatatctct gtcatgaatt aaatcctcat tgtgcaaaga
    17161 tgctcttgat acagttgtac cagctacttt tactggttct aaaatagcag taggtgtgac
    17221 tacacctgtt cgtccaatac ttaattcaat atctaataat ttagttacta cttcctcagc
    17281 tggaaattta taagcaatgg cccatctagg agatttttgt gtgaatccca tctcatcctg
    17341 ttgatctaaa tcattaacct taataacaat cccatcaata tcataaggta atgactctct
    17401 ttggcttgtc catttttcaa tatactctaa aacaccatcg atattattta cacgcgctct
    17461 atttttattc gttgtaaaac ctaatttatc taactcatct aatgcttcac tttgcgaacg
    17521 cgcattgaaa tcagtgaaat cattgacact atatataaat acgcttagct ttcgttttgc
    17581 cgttaattta gaatctaact gtcttaatga tcccgcagca gcgtttcttg gatttgcaaa
    17641 taactgctca tcattttttt ctttttcttc atttaatcgt aaaaatgaac gtctcggcat
    17701 atatgcttca ccacgaactt ctacatttaa tggttctttc attttcaaag gtatcgcatg
    17761 aatcgttttt aaattttcgg taatatcttc acctgttgtt ccatcaccac gtgttaaacc
    17821 ttgaacgaag tatccatcaa catatttcaa tgatactgct aagccatcaa tttttaattc
    17881 gcacatatat tcaacgttgc caatttgttc acgtatgcgt tggtcgaatt ttctcaaatc
    17941 atccacatta aatgcattcc ctaaacttaa cattggcgtg tcatggttga ctttattgaa
    18001 agaggcttgg gcttcaccgc caactctaac tgttggagaa tctacagtct tatactcagg
    18061 atgctcctct tctattttaa tcagttcatg aagtaatttg tcatattcac tatctggtac
    18121 agatggatta tcctctacat agtattcata actgtattga tttaataaat catgtaactc
    18181 gttcacacga gacgataaat cagccatccc ttaatcctcc tttttttcaa ttggtgcaaa
    18241 ttgcgctaac aaacgttttg gcccttgtga tttaaagata atatctagtt cgattgagcc
    18301 atttttctcg tttacattac tcaccatacc ttctccccaa gctttatgca tcactttgtc
    18361 acctacattc caatcagatg acgatacttg ttttttcgtt gacgttgttc gttgactaaa
    18421 tccacgttta gcaaaaggtt ttgccttagg ttgtatcgtt tgtcgtttgc cacttgaatg
    18481 attttctaat agtgattctg gaatttcctt taaaaatcta gatggcatat ttgactgagg
    18541 gcgaccaaat aacattcttg atgtcgcatg agtgatatat aacacctctt cagcccttgt
    18601 gattgctaca taacaaatac gacgttcttc ttgcatttca tgatcatctt cactcttaat
    18661 cgctctaata tgtgggaata aagattcttc catccccatt ataaagacga ttggaaattc
    18721 aagaccctta gccgagtgca tcgtcattag tgttacgcca ttttcagtat ctgcctcatc
    18781 aatatcagct actaacgata aatccgttaa aaagttaatt aatgactgtt cttctaatgg
    18841 ggtattttcc tcatagtctt ttggtactga cataaattca tcgatgtttt ctaatctact
    18901 tcgagattct aatgtatttt cacgttcaag catttctcga tagccagact tttgtaatac
    18961 ttcatcaact atttcatgaa tttctaaaaa ttcttgttct tttatcaagc tttggattaa
    19021 ctcgtaaaaa ttaagacact cttgtgtcac cttttttgac aatccgataa aatcagcttc
    19081 tccaagtgca tcaaacatac tgatattgtt ttgaagtgca tagttttgaa ctttttcaac
    19141 agatgaagga cctacacctc tttttggaac attaataata cgttgcaaac taatgtcatc
    19201 attactattg gcaattatac gcaaataact taataaatct ttgatttctt tacggtcata
    19261 gaacttttgg ccaccaacca ttgtgtatgg catattagat ttcatgaatg tttcctcaag
    19321 tacacgtgat tgtgcattcg ttctatataa aattgccata tcttgatatt tcttaccatt
    19381 acgttgatgc ttcataattt ctcgtattac aaattccgct tcatcacgtt ccgtcattgc
    19441 ttcatagtaa tgaattttct caccattcgt atttgcagtc cacagtcctt ttggcttacg
    19501 ttcagaatta tttttaatca cttcgttagc cgcatttaaa atcgttttcg tcgaacgata
    19561 attttgctct aaaaagattg tattcgcttc tggatagtct ttttcaaatg ataagatatt
    19621 ttgaatatca gcaccacgcc aaccataaat tgactgatct gagtcaccta caacacataa
    19681 gtttttaaac ttacttgcta ataatttaac taatgtgtat tgtgctttat tagtatcttg
    19741 atactcatct acatgaatat attggaattt gttctgataa tattctagaa cttctggtac
    19801 acgctcaaat aagttaatcg ttgtcataat aaggtcatca aaatctaacg cttcattacg
    19861 tgacaattgg cgttgatatc cactataaac cgttgctacc atttgcgagt gataatctgt
    19921 ggcttctttt tgagcatctg caggtgtttt aagttcattt ttcaaattac tgatcgcacc
    19981 gataaacata cgaggttcaa actttttact atcaatattt tcatttttta agacgtcttt
    20041 aataacagat ttttggtctg taggatcaat tatcgtaaaa ttgcgttcta taccaattcg
    20101 atctgcatca cgacgtaaaa tacgaacaca cattgagtgg aatgttgaca tccaaataac
    20161 ttctgcttga tcacctacta atttttgaac acgttctttc atttctcttg cagctttatt
    20221 tgtaaaagta atagccaaaa cattgtatgg tgagacatct ttttcgtcta ataaataagc
    20281 aattctatgt gttaaaacac gtgtcttccc tgaaccagca cctgccataa ttaacaatgg
    20341 tccttctgtt gtctttacag cttcactttg ctctgtattc atatgattta ataacgcatt
    20401 catttactag actcctttat ttttactgtt tttaaagctt ttttaatatc tttataaata
    20461 atatcaccga cgataattgt atctgcaata gctgccatct ctgtcgcttg ttgttctgag
    20521 gaaataccgc caccataaaa aagttgcgtt tctgttagat gttctgagac agcttgaacc
    20581 tttgatacgt cgccataaat gccactatac tctatataca taaccggtaa tcgatacata
    20641 tgattgacca tttgggcata tgcttctaaa tcttctgttg ttaaatctgt atttgctttg
    20701 gtatgttttg ccactttgct atcagcattg cacacgacat acccttcaaa tattacttcc
    20761 tcaaaatcta tactatgtcc atatgtttta agcgcttcta ataatgtacc attgtgaaat
    20821 acaacatctg tactgttcag tactgtaggt acaaaataaa aatcaaaacc aggcattaca
    20881 ctttcgatgt ttgatatttc aagtactaaa ggcaatgggt atcttcttac tctgctcatt
    20941 aaatgaatga cattatcttc agtaacgtca tcagttccac caatcataat tgcatctgtt
    21001 tgagacatac aaatcgcatc taaatcatca tctgaaatat gtttagctgg gtctaattta
    21061 aaaatatggc gccatttttt aatgtcatac attttccgaa actccttttt taacatacat
    21121 atgattatag catttttaag tgtcgagttc taaagactta atatgaaatt taagttcttt
    21181 tactctgtaa attttttcgt gaaaaaacaa tgattatcta ccttattagt gcagatagat
    21241 aaccattgtt tatcgttcat ttattattca tttgtttcaa tattcatacg atctaaaatc
    21301 attgtataag catcattacc ccattgtaat gaacgcttca ctctagaaat cgttgcagtc
    21361 gatgctccag attcttgttc aatcgttgca taggtataac cttgcttaat cattttagca
    21421 acttgtaatc tttgagacag tgattgaatt tcatttacag tacacaaatc atcgaaaaat
    21481 tggtaacatt cttctctatt ttctaacgtt agtattgcat caaacaattc atctaacgct
    21541 gcacctcgta atttttcaat ttgcatatcg aaacaacccc tataatttta ttctttatgt
    21601 cattttaacg cattaatcta ttaaagtgta gcgatttata ttttattaaa tctgaatcgt
    21661 tactttattt aattttatgc tagtccagcg cgttcgaaaa tagtgtcaac ttgattcaaa
    21721 tgatgtttag gatcgaaaca ttcatccaat tcttcttttg ttaaaacact tgtaatagac
    21781 tcatcttgtt cgattaattc acggaacggt gttttcgttt cccaagatat catcgctttt
    21841 ggttgtactt tgtcgtatgc ttcttcacga accatacctt tattaattaa tgctaataag
    21901 acacgttgtg agaaaatcag accaaatgtt ttatctatgt tattacgcat attatcttca
    21961 aatacagtta aacggtccac aatatttgtg aaacgattca atgcataatc tagtgctatt
    22021 gtaacatctg gtaacataat acgctcagca gaagaatgag aaatatctct ttcatgccat
    22081 aatggcacat tctcataagc tgtagtaata taaccacgaa tgactcttga aatacctgtg
    22141 atattttcag agccaattgg atttcgttta tgaggcattg cagatgaacc tttttggcct
    22201 ttcgcaaatg cttcttcaac ttctcttgtt tcggtttttt gaaggttacg aatttcaacg
    22261 gcaaattttt ctagtgacgt cgcgattaat gctaatgtcg caatatagta tgcatgtcga
    22321 tcgcgttgca atgtttgcgt tgatacaggc gctgtgccaa tacctaaatg tttacacaca
    22381 taactttcta tttcaggagg aatgttagca aaagtaccta ctgcaccact cattttccct
    22441 acttcaattt cttctcttac ttgtttgaaa cgttgtaagt tacgttgcat ttccgtgtac
    22501 cacaatgcca ttttgacacc aaatgtagtt ggttctgcat gcaccccatg tgtacgtccc
    22561 atcatcaatg tatatttata attttttgct ttttcagcta aaacgtcgat aaatctttct
    22621 aaatcttttt caataatgtc atttgcttgt ttaataacga aacttaaagc tgtatctaca
    22681 acatcagtag aagttaaacc ataatgtacc cacttacgtt cttcacctag cgtttcagaa
    22741 acttgtctag taaaggctac aacatcatgg cgcgtttctt gttcaatttc ttgtgcacgt
    22801 tcgacattta cctttgcgtt ttgacgaatt ttttgtacgt cagctttcgg tatatgtcct
    22861 aattcactcc atgcttcaca tgctaaaatt tccacttcta accatgcttc atagcgattt
    22921 tgatcagtcc aaatattaga catttcttct ctagaatagc gttcaatcat ttttttaact
    22981 cctatcgtat gtatactcga attttcaaaa acagaacatt tttaataaaa ataaacaaaa
    23041 agttcgtttt taactctact cttcacagtt taacagaatt ggttataaat gtataaaaaa
    23101 tttccgtata ttctcattct attttatttt ttacattaat acaattatct atggcagatt
    23161 aatactgatt tctaattgta aatgttcgca atactttaat ctaattggta tttataatga
    23221 tccattgaga atatattttt gcattttagt ttttctcccc tcaactacac tatccaactt
    23281 tttattacaa ttaggacaat caatagcttt agtaatacca gattttattc actttacttc
    23341 tcctattgta tggaatttct aacaatctac tttacctagt ttcataatac tccttcttac
    23401 atttccataa tatgttttaa aattattcac ttctaatata acaaactttc tactcacaaa
    23461 aagaatatat gttctattgc ataggtatgg cattttaaat ataaggc
//
LOCUS       DAHHYJ010000026        23333 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000026 DAHHYJ010000000
VERSION     DAHHYJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23333
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.026"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(264..533)
                     /locus_tag="NBK22_000782"
     CDS             complement(264..533)
                     /locus_tag="NBK22_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2413985.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            847..1533
                     /gene="mtnN"
                     /locus_tag="NBK22_000783"
     CDS             847..1533
                     /gene="mtnN"
                     /locus_tag="NBK22_000783"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCD2413986.1"
                     /translation="MIGIIGAMEEEVTILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDVIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQIPQMPVAFQSSKPLIEKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            1553..2080
                     /locus_tag="NBK22_000784"
     CDS             1553..2080
                     /locus_tag="NBK22_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCD2413987.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVTSFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            2081..3181
                     /gene="yqeH"
                     /locus_tag="NBK22_000785"
     CDS             2081..3181
                     /gene="yqeH"
                     /locus_tag="NBK22_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCD2413988.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIRDN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDESSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGLITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            3195..4001
                     /gene="aroE"
                     /locus_tag="NBK22_000786"
     CDS             3195..4001
                     /gene="aroE"
                     /locus_tag="NBK22_000786"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCD2413989.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDDKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKSKLTVANRTMARFESWNLN
                     INQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGTESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            4005..4295
                     /gene="yhbY"
                     /locus_tag="NBK22_000787"
     CDS             4005..4295
                     /gene="yhbY"
                     /locus_tag="NBK22_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCD2413990.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATHSELVQVIGSMIVIYRESKDNKEIELP"
     gene            4298..4867
                     /gene="nadD"
                     /locus_tag="NBK22_000788"
     CDS             4298..4867
                     /gene="nadD"
                     /locus_tag="NBK22_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCD2413991.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHHDFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            4857..5441
                     /gene="yqeK"
                     /locus_tag="NBK22_000789"
     CDS             4857..5441
                     /gene="yqeK"
                     /locus_tag="NBK22_000789"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCD2413992.1"
                     /translation="MNIEKAKQLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            5442..5795
                     /gene="rsfS"
                     /locus_tag="NBK22_000790"
     CDS             5442..5795
                     /gene="rsfS"
                     /locus_tag="NBK22_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCD2413993.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYGQVAY"
     gene            5798..6514
                     /locus_tag="NBK22_000791"
     CDS             5798..6514
                     /locus_tag="NBK22_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2413994.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMTLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKHVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            6554..7240
                     /locus_tag="NBK22_000792"
     CDS             6554..7240
                     /locus_tag="NBK22_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCD2413995.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTT"
     gene            7332..7793
                     /locus_tag="NBK22_000793"
     CDS             7332..7793
                     /locus_tag="NBK22_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCD2413996.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            7798..9999
                     /locus_tag="NBK22_000794"
     CDS             7798..9999
                     /locus_tag="NBK22_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCD2413997.1"
                     /translation="MLYVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPIS
                     LFLIIFSSWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEI
                     YPFFLTNKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEK
                     HKQFIMNRIYDSGIKFPDRIMALITGDVKEINEQFKERVKEIGIYHLLAVSGSHIAAI
                     VFLIYKPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLLITKQIKI
                     KGIQLLVFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFI
                     AQLASFIVAIPNFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYL
                     VDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIIFIISVYYIFWLLAKHKYILVTFW
                     TIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYS
                     ISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTL
                     MLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKK
                     VLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKN
                     NMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSNSYGNASGL"
     gene            10056..11030
                     /gene="holA"
                     /locus_tag="NBK22_000795"
     CDS             10056..11030
                     /gene="holA"
                     /locus_tag="NBK22_000795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCD2413998.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(11075..11326)
                     /locus_tag="NBK22_000796"
     CDS             complement(11075..11326)
                     /locus_tag="NBK22_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCD2413999.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            11672..13495
                     /gene="lepA"
                     /locus_tag="NBK22_000797"
     CDS             11672..13495
                     /gene="lepA"
                     /locus_tag="NBK22_000797"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCD2414000.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCVLRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            14059..15183
                     /locus_tag="NBK22_000798"
     CDS             14059..15183
                     /locus_tag="NBK22_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCD2414001.1"
                     /translation="MTVQSSYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     NHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFG
                     QTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND"
     gene            15284..16261
                     /gene="hrcA"
                     /locus_tag="NBK22_000799"
     CDS             15284..16261
                     /gene="hrcA"
                     /locus_tag="NBK22_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCD2414002.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINKLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            16293..16919
                     /gene="grpE"
                     /locus_tag="NBK22_000800"
     CDS             16293..16919
                     /gene="grpE"
                     /locus_tag="NBK22_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCD2414003.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            16988..18820
                     /gene="dnaK"
                     /locus_tag="NBK22_000801"
     CDS             16988..18820
                     /gene="dnaK"
                     /locus_tag="NBK22_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCD2414004.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEEIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK"
     gene            18956..20095
                     /gene="dnaJ"
                     /locus_tag="NBK22_000802"
     CDS             18956..20095
                     /gene="dnaJ"
                     /locus_tag="NBK22_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCD2414005.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPVVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            20099..21037
                     /gene="prmA"
                     /locus_tag="NBK22_000803"
     CDS             20099..21037
                     /gene="prmA"
                     /locus_tag="NBK22_000803"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCD2414006.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            21039..21791
                     /locus_tag="NBK22_000804"
     CDS             21039..21791
                     /locus_tag="NBK22_000804"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCD2414007.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYEKELMG"
     gene            21798..23144
                     /gene="mtaB"
                     /locus_tag="NBK22_000805"
     CDS             21798..23144
                     /gene="mtaB"
                     /locus_tag="NBK22_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCD2414008.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
BASE COUNT         8689 a         3045 c         4346 g         7253 t
ORIGIN      
        1 ctgataataa aactatcaat agtgacaaat tgcatttaga tatctattta ttcaaatcat
       61 aatttaatta agttaatcat taataatttt tgacagtgaa atataaagta cctgtcatta
      121 tactgttaca aataaaaaca gcagtaagga tgataacact tagaaaagtc ttactgctgt
      181 tttctattag aattatcttt aatacacaca ggaattcgat acgaaacaaa tatgattaac
      241 tgtgtaatta caaaataaaa ctactatagt aaaaagaaga taaagacgat gataataaat
      301 gcatcgaata taattttctt gatgttttta tcaacaacat caggtaagga cataagtata
      361 aatcctagaa ttagagtgta gcgacatatg ttcataattg ttgctgtaaa ctcgatatta
      421 aatgaaaact ttaaaaatat acttataaat aaaatgacaa acgacgcaat tgcaataaaa
      481 ctgccgtatg tagctatctt tgttacgaga ctattttgat tattttcagc cattgtaaaa
      541 tcctcttatt tcatcattat tattttaaac atatcatttt cctgttattt aataaagttt
      601 cttgacacga ttggttgatg taattgatat gattagtttt tgaaaaatag acaaattaga
      661 agcatcgaat ttactgttat gagttcaaaa ataattattt atgataccaa gttattgaaa
      721 gagcttttta taaaaataaa gcaaaagttt tatacagtaa tgaaaatcat gatacttgtc
      781 gttgtagtga ttaaaatgtg ataaaataac aaatgctagg attttttaaa agaggtgaat
      841 caaaggatga ttggtataat tggtgccatg gaagaagaag taacaatatt aaaaaataaa
      901 ttaacacaat taagcgaaat ttcagttgca catgttaaat tttatactgg cattttaaaa
      961 gatagagaag tagtgattac ccaaagcggc attggaaaag ttaatgctgc aatttctacg
     1021 acattattaa ttaataagtt taaaccggac gtcattatta atacaggttc tgctggagct
     1081 ttagatgaaa gtttaaatgt aggtgacgtt cttataagtg atgatgtaaa atatcatgat
     1141 gcggatgcaa cagcatttgg ttatgaatat ggacaaatac cacagatgcc ggtagcattt
     1201 caatcaagta aacctttaat agaaaaagta tctcaagttg tacaacaaca acaattaaca
     1261 gctaaagtag gcttaattgt aagtggtgat agctttatcg gtagtgttga acaacgtcaa
     1321 aaaattaaaa aagcatttcc aaatgcgatg gcggttgaaa tggaagcaac tgcaattgca
     1381 caaacatgtt atcaatttaa tgtaccattt gttgtagttc gtgcagtttc agacttagca
     1441 aatggagaag cggaaatgag cttcgaagca tttttagaaa aagcagctgt atcatcaagt
     1501 caaactgttg aagcattagt gtctcaatta taaaaaggta aaggtgagtt aaatgggttt
     1561 agttcgcaag ttttttatgc cgaattcata tgttcaatca atatttcaaa ttgatttaga
     1621 caagttagtg gacaaaggcg ttaaaggtat tattacagat ttagataata cgctagtagg
     1681 ttgggatgtt aaagaaccta cagaacgtgt taaagcatgg tttaaggaag ctaatgaaaa
     1741 aggaatcact attacaatcg tgtctaataa taatgagtct cgtgttacta gttttagtca
     1801 gcatttagac atcgatttta tttttaaagc gagaaagcca atggggaaag cgtttgataa
     1861 agcaataact aagatgaata tcagaccaga tcaaactgtt gttataggtg accaaatgct
     1921 tactgatgta tttggtggta atcgtcgagg tctatataca attatggttg ttccagttaa
     1981 acgaactgat ggctttatta ctaagtttaa tagattaatt gaaagacgat tattacgtca
     2041 tttcagtaaa aaaggttata tcacatggga ggaaaattga ttgtctgaca ttttaaaatg
     2101 tatcggttgt ggtgcaccac ttcaatctga agataaaaat aaacctggtt ttgtaccaga
     2161 gcataatatg tttcgtgatg acgtgatttg cagacgttgt ttccgcttga aaaattataa
     2221 cgaagttcaa gatgtaggat tagaaagtga agacttttta aaattattat caggacttgc
     2281 ggataaaaag ggtattgtcg tcaatgtcgt ggatgtattt gactttgaag gatcatttat
     2341 taatgcagtt aaacgtattg tcggaaacaa aaaaatcatt ttagcagcaa ataaattgga
     2401 tttattgccg aaacaaatta ataaacgtcg agttaaagaa tggttaaaac gaacagcaag
     2461 aaaatatggt ttggaagctg acgatgtcgt attaatttca gctgaaaaag gctggggcat
     2521 agacgactta ttatcatcaa ttgcgaatat tcgagataat gaagatgtgt atattgtagg
     2581 gacaacgaat gttgggaaat ctacattgat taataaactg attgaagcta gtgttggtga
     2641 aaaagatgta gtaacaactt caagattccc tggaacaact ttagatatga tagatattcc
     2701 tttagatgaa tcatcattta tgtatgatac accaggtatt attcaagatc atcaaatgac
     2761 gcatttagtt agtgaaaaag aattgaaaat tattatgcct aagaaagaaa taaaacaacg
     2821 cgtatatcaa ttaaatgagg cgcagacatt attcttcggc ggtctagcgc gcatagatta
     2881 tgtatcaggt ggtaaacgtc cgttagtttg tttcttttct aatgacttga atatacatcg
     2941 tactaaaacg gagaaggcta atgatttatg gcgtaatcaa cttggcgatt tattaacgcc
     3001 acctggaaat ccacaaaatt ttgatcttaa tgaggtaaag gctgttagac ttgaaacagg
     3061 caaagagaaa cgtgatgtta tgatctctgg tctaggctta ataactatag gaccaggagc
     3121 taaagtaatc gttcgtgttc ctaaaaatgt tgatgttgta ttaagaaatt ctattttata
     3181 aggtgattta aaaaatgaaa tttgcagtta tcggaaatcc tatttcacat tccttgtcgc
     3241 ccgttatgca tagagcaaat tttaattctt taggattaga tgatacttat gaagctttaa
     3301 atattccaat tgaagatttt catttaatta aagaaattat ttcgaaaaaa gaattagatg
     3361 gctttaatat cacaattcct cataaagaac gtatcatacc gtatttagat catgttgatg
     3421 aacaagcgat taatgcaggt gcagttaaca ctgttttgat aaaagatgac aagtggatag
     3481 ggtataatac agatggtatt ggttatgtta aaggattgca cagcgtttat ccagatttag
     3541 aaaatgcata cattttaatt ttgggcgcag gtggtgcaag taaaggcatt gcttatgaat
     3601 tagcaaaatt tgtaaagtcc aaattaactg ttgcgaatag aacgatggct cgttttgaat
     3661 cttggaattt aaatataaac caaatttcat tagcagatgc tgaaaagtat ttagctgaat
     3721 tcgatatcgt tattaataca acaccagcgg gtatggctgg aaataacgaa agtattatta
     3781 atttaaagca tctttctccc aatactttaa tgagtgatat tgtttatata ccgtataaaa
     3841 cacctatttt agaggaagca gagcgcaagg gaaaccatat ttataatggc ttagatatgt
     3901 ttgtttacca aggtacggaa agctttaaaa tttggactaa taaagatgct gatattaatt
     3961 ctatgaaaac agcagtttta caacaattaa aaggagaata acatatgctt actggcaaac
     4021 aaaaaagata cttaagaagt ttagcacaca atattgatcc gatttttcaa attggaaaag
     4081 gcggtatcaa cgaaaatatg attaaacaaa tagatgatac gttagaaaac agagaattga
     4141 ttaaagtaca tgtactacaa aataactttg atgataaaaa agaattagct gaaacattaa
     4201 gcgaagctac gcatagtgaa ttagtgcaag tgattggatc tatgatagtg atttatagag
     4261 aatctaaaga taataaagaa attgaattgc cataataatg aaaaagatag tactttacgg
     4321 cggtcagttt aaccctatcc atactgcaca tatgatagta gctagcgaag tatttcatga
     4381 attacagcca gatgaatttt attttttacc tagttttatg tctccattga aaaagcacca
     4441 tgattttata gacgttcagc acagattaac aatgatacag atgattatcg acgagcttgg
     4501 ttttggagat atttgcgacg atgaaattaa acgtggtggt cagagttata cctatgacac
     4561 gatcaaggca ttcaaggagc aacacaaaga cagtgagttg tactttgtta ttgggacgga
     4621 tcagtataac caactagaga aatggtatca aattgaatac ttaaaagaaa tggttacttt
     4681 tgtagttgta aatcgagaca aaaatagtca aaatgttgaa aatgctatga ttgcaattca
     4741 gatacctagg gtagatataa gttcgacaat gattcgacaa agagttagtg aagggaaatc
     4801 tatccaagtt cttgttccta aatccgttga aaactatatt aagggggaag gattatatga
     4861 acattgaaaa agcaaaacag cttgcaaaag agaaactgcc agagaaacgt tataatcatt
     4921 ctttaagagt tgcagaaaca gcgattaaat tggcagaaat ttatgatgga gacactagta
     4981 aagtagaatt agcaggtgta ttacatgatt tctgtaaata tgatgattta ggtaaaatgt
     5041 atcaaattgt tcgacaatac gaattaggta atgatctact gagttatggt agtgaaatat
     5101 tgcatggccc tgtgtgtgca gcaatcatgg aacatgaata tggtatcaat gatgaggaag
     5161 tattaatggc tatcaaatac catactactg gacgtcaaca aatgacaaaa actgaaaaac
     5221 tgatttttat tgctgattac atcgaacctg gaagaacaat cccaggagtt gatgatattc
     5281 gagatatggc atacaatcaa ggtagtttag ataagacaat ttatgaaatt tctaaacgca
     5341 cagtactatt tttaatacag aaagatatta cggtatataa taagacgatt gactgtttaa
     5401 attattataa ctatagtgac gaaagaataa aggatgatta aatgaattca caagaattat
     5461 tagcaattgc tgtggatgca attgacaata aaaaaggcga agatacgatt tctttagaaa
     5521 tgaaaggtat cagcgatatg acagattatt ttgttgtaac gcacggaaat aatgaacgac
     5581 aagttcaagc gattgctaga gcggtgaaag aagtagccaa tgaacaaaat atagaagtaa
     5641 aacgtatgga aggatacaat gaagcgcgtt ggatattaat tgacttagct gatgttgtgg
     5701 tacatgtttt ccataaagac gaaagaaatt attataatat tgaaaagtta tatcaagatg
     5761 caccattaga atcatatggt caggttgcgt attaattatg tcgcaatatg cagaaatgag
     5821 cctagtgtac gatcaattga ctcaagatca accatatgaa aaatggtttg aaattgtaaa
     5881 aaatcactgc aaagatgaat caaatatttt agatattgga tgcggtactg gtagtttaac
     5941 agttcaatta gaagctttag gtaatgttac tggtatggat ttaagtgttg atatgttgac
     6001 tgttgcagct caaaaatcag caaatgtaaa ttggcttgaa ggagatatga catcttttga
     6061 tttgcaacaa caatttgata tcatcactat tttttgtgac tctctaaatt atttgcaaga
     6121 tgaaactgca gtgattgaaa cattcatcaa tgtttatcat catctgactg attcaggtgt
     6181 atttattttt gatgtacata ctgtttataa aatgatgact ttgtttaata atcaaagtta
     6241 tattgatgat aaaggggaca tttttttagc atgggatgct gtgcaaggag atttaccttt
     6301 aagtgtttat catgatatga catttttcat tcgacatgaa gatgaaacat attcaagatt
     6361 tgatgagtct cattttcaga gaacatttga tgaaaagaca tatttgtctt ggttagctca
     6421 agttggtttt aaacatgttg aaacatttac tgattttaat atagatgaac ataatgaaga
     6481 tgcagaaaga ttgtttttca ttgcgaaaaa ataaaagtaa aaccactcgc tcagacgtgt
     6541 ataaagttga ggagtggttt tattgtatca atttttatta cgctataaag attttttaac
     6601 tcagtggaag ttatatatta taagtgctgt tgttttaatt atggtattaa ttggttttat
     6661 attctggaga caagatgatt atacttcaag aaattttgaa aataaagata ctgctctgaa
     6721 acaaagcact agtgaaaata atagtttgtc caaattagaa gatgtccagg tcaaagatgg
     6781 agataattcc aaaaataaag gtcctgtata tgtcgatgta aaaggtgctg ttaaacatcc
     6841 taatgtttat aaaatgacat ctaaggatag agtagttgat ttacttgata aagcacaatt
     6901 attggatgat gcagatgtaa gtcgaattaa tttgtctgaa aaattaacag atcaaaaaat
     6961 gattttcata cctcataaag gacaaaagaa tgttgaacca caaattgaag taaacagtgt
     7021 gcacgtaaaa aatgggaaca caaataatac taaagtaaat ttaaatacgg catctgtatc
     7081 agaattgatg tctgttcctg gagtagggca agctaaagct aatgcaattg ttgaatatcg
     7141 caaccaacaa ggtgcatttc aagaaattga cgatttgaaa aaagtaaaag gttttggaag
     7201 taaaactttt gataaactga aatcttattt cacgacataa tggattattt taattgcaaa
     7261 ttttattttt tgtgattaat cttatattat tagaaaatgt tactaaattt taagtatgga
     7321 aggtgaacat cttggaaaga atcaaatggg aagaatattt tatggcacaa agtcatttgc
     7381 tagcattacg ttcaacttgt caaagattat ctgtaggtgc aacgattgtt aaggataatc
     7441 gtattattgc tggtgggtat aatggctctg tagctggcga ggtgcattgt atagatgaag
     7501 gatgtttaat tgaagatgga cattgtatca gaacgataca tgcagaaatg aatgctttat
     7561 tacaatgtgc aaaacaaggt gtatctactg aaggtgcaac aatctatgtt actcattttc
     7621 catgcctaaa ttgtacaaag tcaattattc aagcaggtat aaagcgtatc tactatgcag
     7681 aagattatca taaccatgaa tatgcaacta aattactcaa acaatctggt attgaattta
     7741 aaaaaattcc attttcacca gaatatgttg ctaaatatct gactaaaggt taatgttttg
     7801 ctgtatgtcg cgttatcaat gattgtagga gtgctttgga attctagcaa agtgctctct
     7861 acatttcttt tcattttact tttgtatatt acttatcgta aaaataaaat cgtttatgcc
     7921 cctatttctc tctttttaat cattttctcc tcatggtatt tacattattc acaacaagca
     7981 atatttaatt atatcaatta tattgaacgt aattctcagt ttaatgagcg tgctcaagta
     8041 atccaaattc aacgtcaagg tagtgacaca tataaaggta ggttgagttt aaaaaatgaa
     8101 atatatcctt tctttttaac aaataaaaag aattttgatt taaagaaaat tgaaagtcgt
     8161 aattgtattg ttaaaggaca attcaaagtt aatgacaata agtttgtaac tcttaaatta
     8221 caaagtatag ttgtacaaag ctgcctagaa tcgaaccggt ctaatttaat tgagaaacat
     8281 aaacagttta taatgaatcg aatttatgat tcgggtatta agtttccgga tcgtattatg
     8341 gcattgatta ctggtgacgt aaaagaaatt aatgagcaat ttaaggaacg tgttaaagag
     8401 ataggtatat atcatttgct ggcagttagt ggctcgcata tagctgcaat tgtattctta
     8461 atttacaaac ctttaaaacg attaaattta cctttatttg tcattaaagg aattacaatc
     8521 attgtattag ctttatttgc tcaatacaca aattatgcac ctagtgctgt aagagctata
     8581 ataatgacaa ctcttgtact gcttattact aagcaaatta aaataaaggg tattcagcta
     8641 ttagtatttg catttataat tatgtttatt ttaaatccac tagttgttta tgatattgga
     8701 tttcaatttt cattcatcat ttcatttttt attatgctac tttttccttt tttacagcaa
     8761 ttgtcaaagt tacaatcatt attcataatt acgtttattg cacaattagc ttcatttatc
     8821 gttgccattc caaactttca tcaacttcaa tgggtgggat ttttatctaa tttgattttt
     8881 gtaccgtact attcgattat attgtttccg ctatctattt tattctttat tacaagtcat
     8941 tttattgtgg gattaacgcc gctaaattac ttggttgacc taagttttaa ttttcatgac
     9001 tggttactag acctattcac aagaattaag caatcacatt tttctgttcc caagtttaat
     9061 gattggatat ttataatatt tataatttct gtttattaca tattttggtt attggctaaa
     9121 cataaatata tattggttac gttttggact ataattattc tgacattatt aataacgttt
     9181 ccaacaaatt cacatcacaa aattacaatg ttaaatgtgg ggcagggaga cagtatttta
     9241 tatgaaggtg gtaagaacca aaatgtcttg attgatacag gtgggaaagt gattgatgat
     9301 actaaacaac ctagttattc aatttctaaa tatcatattt taccaacgct aaatgaaaga
     9361 gggataaatg aattagagta tctaatttta acacatccac acaatgacca tattggtgaa
     9421 gtggaatata ttattagtca tattaaaatt aaacatatag tgatatacaa taagggatat
     9481 agtagtaata cattgatgtt attatcgaaa ttaagccata agtataacat taaacttatg
     9541 gatgtaagac aagttagtag ttttaaactt ggagatagta gttttctatt ttttgatagt
     9601 tttattccaa atagccgaga taaaaatgaa tattctatta ttactatgat tacatatcaa
     9661 aataaaaaag ttttattaat gggcgatgct agtaaaaata atgaatcttt actactaaaa
     9721 aaatataact tgccggagat tgatatttta aaagtaggac atcatgggag caagacaagt
     9781 agttctaaag aatttataga gatgattaag cctaaaataa gtttgatttc ttctgggaag
     9841 aacaatatgt atcatcttcc taatatagaa gttgttaaac gattgcaaag gattcgcagt
     9901 cgcatttaca atagtcaaca aaacggtcaa gttacaattg acttagatga taatttaaaa
     9961 gttgattcaa actcttatgg aaatgcaagt ggtttataga tacagaaaca tgatataatc
    10021 tattgatgtg tcaaacgaat gaaagggtgt aatgaatgag cgacaatatt gtagctattt
    10081 atggagatgt gcctgaattg gttgaaaaac aaagtgcaga aatcatatca caatttttga
    10141 aaagtgatag agatgacttt aactttgtga aatataattt atacgaaaca gagattgcac
    10201 caattgttga agaaacatta acattgcctt tcttttcaga taaaaaagca attttggtta
    10261 aaaatgcata tatatttaca ggtgaaaaag cgccaaaaga tatggctcat aatgtagatc
    10321 aattaataga atttattgaa aaatatgatg gcgaaaattt gattgtcttt gagatatatc
    10381 aaaataaact tgatgaaaga aaaaagttaa ctaaaacttt aaaaaagcat gcaaggctta
    10441 aaaaaataga gcaaatgtct gaagaagaaa taaaaaaatg gattcaaagt aaattaaatg
    10501 agaatttcaa agatatcaaa agagatgcat tagatttatt tattgagttg acaggtatta
    10561 actttaatat tgtctcacaa gagatagaaa agttgatttt atttttaggc gatagaccaa
    10621 caattaataa gcaggatgtt aaccaaatta ttaatagaag tttagagcaa aatgtatttt
    10681 tactgactga atacattcag aaaagaaaga aagaacaagc aattcattta gtaaaagatt
    10741 taataactat gaaagaagaa ccaattaaat tacttgcact aattacaagt aattaccgat
    10801 tattttatca atgtaagatt ctgagtcaaa aaggatatag tggacagcaa attgctaaaa
    10861 caataggagt acatccatac agagtaaaat tagcgttagg acaagtaaga cattatcaac
    10921 ttgatgaatt attgaatatt atagatgctt gtgcggaaac tgattataaa cttaaatcat
    10981 catatatgga taaacagtta atactggaat tattcattct atctttataa aaaataaaag
    11041 ttcaagcatg cgcttgaact tttatttaaa aagattattt atttgcagtc attaattgtg
    11101 atttaatacg gtcagctttg tttgaatgta ttaaattact ttgagcagct ttgtctacta
    11161 acttaactgc taagcttact aattcatttt tattatcagc gttatttgaa acagctgttt
    11221 tagcgttttt aactgctgta cgcattgcac tcttttgtga aatgttgcgt gcttcagctt
    11281 tttcagttgt ttttacacgt ttaattgcag atttgatatt tgccatttct gtcacctcct
    11341 aaaagtgagt tcatcttatc aaaattttat ttgattacaa caagaaatat tttaacaaaa
    11401 gtctaaccgt tgtgcaatca tttatttgat ggggttagct taaaattttg cagatttaat
    11461 aaattgtgtt ggaaacacaa tcaatacgtt ttataattat aagtacgtaa ttaacatatt
    11521 aacatatcgc aagtatgtat ttaaataaag attgttataa tttcaaagtt catccaagat
    11581 tatggcgttt gcatttacct attaaaaacg ttattatatc aaagatgcga aagataatac
    11641 gggtttattt tatgaaagtg agaaggataa aatggataat gagcaacgct taaaaagaag
    11701 agagaatata aggaatttct cgattatagc acatattgac cacggaaaat ctacattggc
    11761 tgatagaatt ttagaaaata ccaaatcagt tgaaacaaga gatatgcaag atcagttact
    11821 agattcaatg gatttagaaa gagaacgtgg tattacaatc aaattaaacg cagtacgttt
    11881 aaagtacgaa gctaaagatg gaaatactta tacattccat ttaatcgata cgcctggaca
    11941 cgtcgatttt acatatgaag tgtcacgttc tttggcagct tgtgagggcg cgattttagt
    12001 agtagatgcg gctcaaggta tcgaagcaca aacattagca aatgtttatt tagcattaga
    12061 taatgagtta gagttattgc ctgttattaa caaaattgat ttacctgctg cagaacctga
    12121 acgcgtgaaa caagaaattg aagatatgat aggtttagac caagacgatg ttgttttagc
    12181 aagtgctaaa tctaacattg gaattgaaga gatactagag aaaatagttg aagttgtgcc
    12241 agctccagat ggtgacccag aagcaccact aaaagcgtta atatttgatt ctgagtatga
    12301 tccatataga ggggtaattt catcgataag aattgtggac ggtgttgtta aagccggaga
    12361 taaaattcga atgatggcca ctggtaaaga gttcgaagta acagaagttg gaattaatac
    12421 acctaagcag cttccagttg atgaattaac agttggtgat gttggttata ttattgcaag
    12481 tattaaaaat gttgatgatt ctagggttgg tgacaccatc acattagcta gtagacctgc
    12541 atcagaacca ttgcaaggtt ataagaaaat gaatccaatg gtatattgcg gactgttccc
    12601 aatagataac aaaaattata atgatttaag agaagcatta gaaaaattac aattgaatga
    12661 tgcatcatta gaatttgagc ctgaatcgtc acaagcatta ggttttggtt atagaactgg
    12721 tttcttaggt atgttacaca tggaaataat tcaagaaaga attgaaagag aatttggtat
    12781 tgaattaatt gcaactgcac catctgtaat ttatcaatgt gttttaaggg acggttcaga
    12841 agtgacggtt gataacccag cacaaatgcc agatcgtgat aaaattgata aaatatttga
    12901 gccatatgtt cgagcaacta tgatggttcc aaatgactat gtcggtgcag taatggaatt
    12961 atgtcaacgt aaacgtggac aatttataaa tatggactat ttagatgata ttcgtgtaaa
    13021 tattgtttat gaattacctt tagctgaagt tgtatttgat ttcttcgatc aacttaaatc
    13081 taatactaaa ggatatgcat catttgatta tgaattcatc gaaaataaag aaagtaattt
    13141 agtcaagatg gatattttat taaacggtga taaagtggat gcgctaagct tcatagttca
    13201 tagagatttt gcatatgaac gtggtaaagc attagttgaa aaacttaaaa cgttaattcc
    13261 aagacagcaa tttgaagtac ctgtacaggc tgcaatagga caaaaaattg tagcgcgtac
    13321 aaatattaaa tcaatgggta aaaacgtttt agctaaatgt tatggcggtg acataagccg
    13381 taaacgtaaa ttacttgaaa aacaaaaagc aggtaaagct aagatgaaag cagttggtaa
    13441 tgttgaaatt ccacaggatg ctttcttggc tgtattgaaa atggatgacg aataattttt
    13501 tgaaaatcaa tctacaattt acaatgaata aagtttaata actaaaaaga gggagcctag
    13561 gataaattaa cgtcctgggc tttacaatgt tatattggca gccatcgaca gagttaaaat
    13621 gagcttataa caatggggcc ccaacataga gaatttcgaa aagaaattct acaggcaatg
    13681 caagttgggg tggggcccca acatagagaa attggattgc caatttctac agacaatgca
    13741 agttggggtg ggacgacgaa ataaattttg cgaaaatatc atttctgtcc cactccctta
    13801 tgcatgagtt ttactcatgt aattctattt ttaagtacat attacctctg gctaatgtgt
    13861 aagagccact acataataaa tcattagagg tactttatta tttctatctc actctctctt
    13921 aacatttata aatatcaaaa tgaatcgata tattagacaa tcattgatta aacgttaaag
    13981 ttaaaagtaa gaataattgc agatagtcca acaggatata gccgattgga taaacagtct
    14041 gagaagcggg gtattaaaat gacggtacaa agttcatata tacatattcc attttgtgta
    14101 agaatatgta catattgtga tttcaataaa tattttatac agaatcaacc tgtagatgag
    14161 tacttagatg cactaataac agaaatgtct acagcaaaat ataggaactt aaagaccatg
    14221 tatgtaggtg gcggcacacc aacggccctt tctattaatc agttggaaag attacttaaa
    14281 gcaatacgtg atacgtttac aatcacaggc gagtatacat ttgaagcaaa tcctgatgag
    14341 ttaactaaag agaaagtcca actattggag aaatatggag taaacagaat ttcaatgggc
    14401 gttcaaacat tcaagccgga gttattgtct gttttaggta gaacgcacaa tactgaagat
    14461 atttacactt cggtgttaaa tgctaaaaac gcaggtatta aatcaatcag tttagattta
    14521 atgtatcatt taccgaaaca gacgattgaa gattttgaac aaagtttaga tttagcttta
    14581 gatatggata ttcaacatat ttcgagttac ggcttaatac ttgaacctaa aacccaattt
    14641 tataatatgt atagaaaagg gttgctcaaa cttcctaatg aggatttagg tgctgacatg
    14701 tatcagttgc tgatgtctaa gatagaacaa tctcctttcc atcaatacga aatatctaat
    14761 tttgcattag atggccatga atcagaacat aataaggttt actggtttaa tgaggaatat
    14821 tatggatttg gagcaggtgc aagtggttat gtagatggtg tgcgttatac gaatatcaat
    14881 ccagtgaatc attatatcaa agctataaat aaagaaagta aagcaatttt agtatcaaat
    14941 aaaccttctt tgactgagag aatggaagaa gaaatgtttc ttgggttgcg tttaaatgaa
    15001 ggtgtgagta gtagtaggtt caaaaagaag tttgaccaat ctattgaaag tgtctttggt
    15061 caaacaataa ataatttaaa agagaaggaa ttaattgtag aaaagaacga tgtgattgca
    15121 cttacaaata gagggaaagt cataggtaat gaggtttttg aagctttcct aataaatgat
    15181 taaaaaaaat tgaaatttcg agtctttaac attgacttac tttgaccaat ttgataaatt
    15241 ataattagca cttgagataa gtgagtgcta atgaggtgaa aacatgatta cagataggca
    15301 attgagtata ttaaacgcaa ttgttgagga ttatgttgat tttggacaac ccgttggttc
    15361 taaaacacta attgagcgac ataacttgaa tgttagtcct gctacaatta gaaatgagat
    15421 gaaacagctt gaagatttaa actatatcga gaagacacat agttcttcag ggcgttcgcc
    15481 atcacaatta ggttttaggt attatgtcaa tcgtttactt gaacaaacat ctcatcaaaa
    15541 aacaaataaa ttaagacgat taaatcaatt gttagttgag aatcaatatg atgtatcatc
    15601 agcattgaca tattttgcag atgaattatc aaatatatct caatatacaa ctttagttgt
    15661 tcatcctaat cataaacaag atattatcaa taatgtacac ttgattcgtg ctaatcctaa
    15721 tttagttata atggttatcg tattttcatc aggtcatgtt gagcatgtac atcttgcttc
    15781 agacataccg tttaataatg acaaactaaa tacaatatca aattttgtta caaacaaact
    15841 aactgagttt aatcagaatt tacaagatga tattgtctct tttgttcaat cagagcagga
    15901 agaaatattt atcaataaat tgttaaatac gatgaataat catatttcta atcaaagtaa
    15961 tagcatttat atgggtggaa aggttaaact tattgatgca ttgaatgaaa gtaatgtatc
    16021 ttcaattcag cccattctac aatatataga atcaaataga attgcagaat tattacaaga
    16081 tatatcttca ccaaatatta atgtaaaaat tggtaatgaa attgatgaca gcttaagtga
    16141 tatttcaatt gtaacaagtc aatatcattt tgatgaaacg ttaaaaggtc aaatcgctgt
    16201 gattggacct acagctatgc attatcaaaa tgtcattcaa ttattaaata gaatttggta
    16261 atgatggatt agaaagtatt ggaggacaga aaatgacaaa taaagacgaa tcagttgaaa
    16321 aaaacactga atcaacagtt gaagaaacaa acgtcaaaca aaatattgat gattcagttg
    16381 aacaagctga agaaagtaaa ggtcatttac aagatgaagc aatagaagaa acgtctgacg
    16441 agaatgttat tgaagaaata gatccaaaag atcaaaaaat taatgaactt caacaattag
    16501 cagatgaaaa cgaagagaaa tatttaaggc tctacgctga gtttgaaaat tataagcgta
    16561 gaattcaaaa agaaaatgaa ataaataaaa catatcaagc acaacgtgtg ttaacagata
    16621 ttttaccagc aatagacaat atagaacgtg cacttcaaat tgaaggtgat gatgagactt
    16681 ttaaatctct tcaaaaaggt gtacaaatgg tgcatgaaag tttgattaac gcactaaaag
    16741 ataatggtct tgaagttatt aaaactgaag gtgaagcatt tgatccaaat attcaccaag
    16801 ctgtagttca agatgataac cctgattttg aatctggcga aatcactcaa gaactacaaa
    16861 aaggatacaa gcttaaagat agagtattaa gaccatcaat ggtcaaagta aaccaataac
    16921 ttaaatttgg cgaaaagaca ttgtttaaaa ttaaattaat ttaatgatta aatggaggaa
    16981 ttttattatg agtaaaatta ttggtataga cttaggtaca acaaattcat gtgtaacagt
    17041 attagaaggc gatgagccaa aagtaattca aaaccctgaa ggttcacgta caacaccatc
    17101 tgttgtagct ttcaaaaatg gagaaactca agttggtgaa gtagctaaac gtcaagctat
    17161 tacaaaccca aacactgttc aatctattaa acgtcatatg ggtactgatt ataaagtaga
    17221 tattgaaggt aaatcataca caccacaaga aatctcagct atgattttac aaaacttaaa
    17281 aaatacagct gaaagctatt taggtgagaa agttgacaaa gctgtaatta cagtacctgc
    17341 atactttaac gatgctgaac gtcaagcaac taaagatgct ggtaaaattg ctggtttaga
    17401 agttgagcgt atcattaatg aaccaacagc tgcagcatta gcatatggtt tagataaaac
    17461 tgataaagat gaaaaagttc ttgtttttga cttaggtggc ggtacatttg acgtatctat
    17521 cctagaatta ggtgacggtg tattcgaagt actatcaaca gccggtgaca acaaacttgg
    17581 cggtgatgat tttgaccaag taattattga ctacctagtt gcagaattca aaaaagaaaa
    17641 tggcgtagac ttatctcaag ataaaatggc attacaacgt ttgaaagatg ctgctgaaaa
    17701 agctaaaaaa gacttatcag gtgtatcaca aactcaaatc tcattaccat ttatctcagc
    17761 tggtgaaaac ggtccattac acttagaagt aaacttaact cgttctaaat ttgaggaatt
    17821 atcagattca ttaattagaa gaacaatgga acctacacgc caagcaatga aagacgctgg
    17881 cttaacaaac tcagatatcg atgaagttat cttagttggt ggatcaactc gtattccagc
    17941 agtacaagaa gctgtcaaaa aagaaatcgg taaagaacct aacaaaggag taaacccgga
    18001 cgaagtagtg gcaatgggag ctgcaatcca aggtggcgtt atcacaggtg acgttaaaga
    18061 cgtagtatta ttagacgtaa caccactatc tttaggtatt gaaattttag gtggacgtat
    18121 gaatacgtta attgaacgta acactacgat tcctacatct aaatctcaaa tctattcaac
    18181 agcagtagat aatcaaccat cagtagatgt acacgtatta caaggtgaac gtccaatggc
    18241 tgcggataat aaaacacttg gtagattcca attgactgat attccaccag ctgaacgtgg
    18301 taaacctcaa attgaagtaa cgtttgatat cgataaaaac ggtattgtaa atgtaactgc
    18361 aaaagactta ggtacaaata aagaacaaag aattacaatt caatcaagtt cttcattatc
    18421 agacgaagaa atcgaccgta tggtaaaaga tgctgaagtt aacgctgaag cagataaaaa
    18481 acgtcgtgaa gaagtagact taagaaacga agctgacagt ctagtattcc aagttgaaaa
    18541 aactttaact gatttaggcg aaaatatcgg tgaggaagat aaaaaatctg ctgaagagaa
    18601 aaaagacgct cttaaaactg ctttagaagg tcaagatata gaagaaatta aatctaaaaa
    18661 agaagaactt gaaaaagtga ttcaagaatt atcagcaaaa gtatatgagc aagcggctca
    18721 acagcaacaa caagcacaag gtgctaatgc tggtcaaaac aatgatagta ctgtagaaga
    18781 tgctgaattt aaagaagtaa aagacgacga caaaaaataa ttaaacaata caattagaat
    18841 gttgtcatga tctgaaaagt caaagtcaat agaacattgg ctttggcttt ttatttggat
    18901 agtatgacat attacgttaa actatatgag ttaattaaga aaaggagaga tagctgtggc
    18961 caaaagagac tattatgaag ttttaggcat tagtaaggat gcttcaaaag atgaaatcaa
    19021 aaaagcgtat cgaaagcttt cgaaaaaata tcatccagat attaacaaag aagaaggtgc
    19081 agatgaaaag tttaaagaaa tttctgaagc ctatgaagtt ttaagtgatg ataataaacg
    19141 cgcaagctat gatcaatttg gacatgacgg gccacaaggt tttggtggac aaggattcaa
    19201 tggctctgac tttggtggtt ttagcggctt cggtggcggc ggttttgaag atatctttag
    19261 ctctttcttc ggtggcggca gacaaagaga tccaaatgca ccgcaaaaag gtgatgatct
    19321 tcagtataca atgacattga catttgaaga agcggtattt ggtacaacaa aagaaatttc
    19381 aatccgtaaa gatgtaacat gcgaaacatg tcatggtgat ggtgcaaagc ctggaacaag
    19441 taaaaagact tgtagttact gtaatggcgc tggtcatgta gctgtagaac aaaacacaat
    19501 tttaggtaga gttcgtactg aacaagtttg tcctaaatgt aatggaagtg gtcaagaatt
    19561 tgaagaagct tgtccaacat gtcacggaaa aggtactgaa aataaaacag ttaaattaga
    19621 agtgaaagta cctgaaggtg tcgacaatga acaacaaatt agattagctg gtgaaggctc
    19681 tccagtcgtg aatggaggac ctgctggtga tttatacgta gtatttagag ttaaaccatc
    19741 tgaaacattt aaacgtgatg gtgatgatat ttattacaaa ttaaatgtta gcttcccaca
    19801 agcagcttta ggcgatgaaa taaaaatacc aacattaaat aatgaagtta tgttaacaat
    19861 ccctgctggt acacaaacag gtaagcaatt ccgcttaaaa gaaaagggta taaaaaatgt
    19921 tcatggctat ggatacggag atttatatgt tgatattaaa gtagtaacgc ctactaaatt
    19981 aacagataga caaaaagaac taatgaaaga atttgcccaa ttaaatggtg aagaaattaa
    20041 tgaacaacct tcgaatttta aagatagagc aaaaagattc tttaagggag aataaagtat
    20101 gaactggaca gagctttcaa ttattattaa tcatgaagca gtagaattgg ctaccaatat
    20161 acttgaaaat catggatcaa atggtgtcgt gatagaagat tcagatgatt taattaacca
    20221 accagaagat aaatacggtg aaatttacgc tttgaaaaaa gaggattatc cagataaggg
    20281 agtaagattg aaagcctatt ttaatgaaat gacttatgat gataagttgc gacagcaaat
    20341 taaagatgag ttattaaatt tagatgaact tgatcaacat aacgttcaat tcagtgagca
    20401 aattattgca gagacggatt gggaaaatga atggaaaaac tatttccatc cattccgagc
    20461 gtcgaagaag ttcacaatag ttcctagttg ggaaacatat gctaaagaag cggatgaaga
    20521 gctttgcatt gagctcgacc caggtatggc ttttggaaca ggcgatcatc cgactacaag
    20581 tatgtgtttg aaggcaatag aaacatatgt attgccacag cattcagtaa ttgatgttgg
    20641 tactggctca ggtatattaa gtattgcaag tcatctaatc ggtgtaaaac gtattaaagc
    20701 gttggatatt gatgaaatgg cagtgagtgt agctaaagaa aacttcagaa gaaatcattg
    20761 tgaaacgtta attgaagctg ttccaggtaa cttattgaaa gacgaaacag aaaaatttga
    20821 tattgtaata gcaaatattt tagcgcatat tattgatgaa atgattgaag atgcttataa
    20881 tactctaaat gaaggcggtt attttattac ttctggtatt ataaaagaga agtatgaagg
    20941 tatacagtca catatggagc gtgtaggttt taaaattatt tcagaacaac atgacaacgg
    21001 ttgggtttgt cttgttggcc agaaagtgag tgaataatgt gcaacgttat ttcatagacc
    21061 aaaacgctga tgtaagtcag cgttttttta ttacaaaaaa agaagatatt catcatataa
    21121 caaatgtaat gagatatgat gttggtagta aaattatttt aacttttaat gatcaaacag
    21181 tttatttatg tgaaattgct gacataacaa gtgataaaat tgcagttgat ttaaaagaaa
    21241 aacaaaatat taatacagaa ttgccagttg atgttacgat ttgcagtgga ctaatcaaag
    21301 ctgacaaata tgagtggttg ctacaaaaag ctactgaatt gggtgcttca tcatttatag
    21361 ctgtgagcat ggaacgttca attgttaaat taaacgaggc aaaagttgcc aaaaagatag
    21421 agcgttggca aaaaataatt aaagaagctg ctgaacaaag ctatcgttta gtgattccat
    21481 ctattcaatt cgagtcgaat ttaaaattaa tttgtgatac tattgataat tatgactata
    21541 ttcttattgc atatgaagag gaagcaaaag atggtgagtt aagcaatttc aagcaaactt
    21601 tacaacaatt caaggctcag gataaagtgt tgatgatttt tggtcctgag ggtggtttgt
    21661 cagaaaatga aatttcatta tttagtaata ctagtacagt tgttggtctt ggaccgagaa
    21721 ttttacgggc agaaactgcg ccattgtatg cgttaagtgc aataagttat gaaaaagaat
    21781 taatggggtg aatatttatg tcaacagttg cgtttcacac attaggttgt aaagtaaacc
    21841 attatgaaac tgaagcaatc tggcaattat ttaaagaagc aaactatgag cgcgttgact
    21901 ttgaagcgaa tgctgatgta tttgttatta atacttgtac agtaacgaat acaggtgata
    21961 aaaaaagtcg tcaaataatt agacgtgcaa taagacaaaa tcctgatgct gtaatctgtg
    22021 taacaggttg ttatgcgcaa acttcatcag ctgaaattat ggaaattcct ggtgtcgatg
    22081 tagtagttgg tacacaagat agacataaac tattaggtta cattgatgaa ttccgtaaag
    22141 aacgccaacc aattaatggt gttggaaata tcatgaaaaa tcgtaaatat gaagaattag
    22201 atgtcccata ttttacagat agaacacgtg cgtcattaaa aattcaagaa ggttgtaaca
    22261 acttctgcac attctgtatt attccatggg ctcgtggctt aatgcgttca agagatccgg
    22321 aaaaagtagt tgaacaagcg acgcaactag tgaattcagg atataaggaa attgtattga
    22381 cgggaattca tacaggtgga tatggtcaag atttaaaaga ttataacttg gcccaattat
    22441 tacgtgatct tgaaacgatt aatggattag aacgaattcg aatttcttca attgaagcaa
    22501 gtcaacttac agatgaagta attgacgttt tagaacgttc aacaaaagtt gtgcgtcatt
    22561 tgcatattcc attacaatct ggttcagata cagtattaaa acgtatgaga cgtaagtata
    22621 caatggatag attttcagaa cgattaacaa aattgcataa agctttacca gacttggcag
    22681 ttacgagtga tgtaattgtt ggtttcccag gtgaaactga agctgagttc caagaaacat
    22741 atgattttat cgtaaagcat aagttctctg aactgcatgt tttcccttat tctcctagaa
    22801 ttggcacgcc agctgcaaga atggatgatc aaattgatga agaaattaaa aatgaacgcg
    22861 tgcataagtt aattacgcta agcaatcaac tcggaaagtt atatgcgtct aaatttgatc
    22921 aagatgtgct tgaagtaatt cctgaggaac agggtgacac agaaggtaca ttagttggat
    22981 atgcagataa ttatatgaaa gtacaatttg aaggtgacga atcactcata ggtcaaattg
    23041 taaaagttaa aattacgcaa gcaaattatc cattaaatga agggcaagca attaaagttg
    23101 ttgattttgc aacaaataaa tctgatagag aagttttagt ttaagtattc cttgattcat
    23161 accataaggt tccgtattaa agatataggt tttgtaacgt tgttttaacg gcttcacaat
    23221 ttactatata agcaactctt ctcattaaat aaattattaa gagattttta gaacaaaatc
    23281 taaaagtctt tgtgattttg atgtgaaata tgtttcaaat aaaaaacaat tta
//
LOCUS       DAHHYJ010000027        23206 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000027 DAHHYJ010000000
VERSION     DAHHYJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.027"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            202..1026
                     /locus_tag="NBK22_000806"
     CDS             202..1026
                     /locus_tag="NBK22_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD2414009.1"
                     /translation="MIENKKTVEDTYSTGAIVDSISSSVQMKQVMVQQTPGRYMLKAM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLILVVLTNSELLTSNFMY
                     FTVGWYYKVVSVKKMTWILLYCFLGNILGGFVLFFLMKFAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVVVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIVFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            1259..1795
                     /locus_tag="NBK22_000807"
     CDS             1259..1795
                     /locus_tag="NBK22_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2414010.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEEAFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFDETILGQPPF"
     gene            2025..2756
                     /locus_tag="NBK22_000808"
     CDS             2025..2756
                     /locus_tag="NBK22_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCD2414011.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIQSGVDHFKIVPLLIFSAMHYLKDIPNIVHEMKRRYPDIKVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQICAQNLQISKPT
                     YARTLYGEISFTHDLESISKRYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHFTP
                     SINFDPILKQIINDRLESLMIPMKI"
     gene            2822..5227
                     /locus_tag="NBK22_000809"
     CDS             2822..5227
                     /locus_tag="NBK22_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2414012.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQSVTTANGIEVSY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEQMMNIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETNGYKGSTTFTSLKVSGCDLYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKIFLKDGNVVGAVLYGDIDDGSRFYKMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            5231..5545
                     /gene="nirD"
                     /locus_tag="NBK22_000810"
     CDS             5231..5545
                     /gene="nirD"
                     /locus_tag="NBK22_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCD2414013.1"
                     /translation="METKEKIKVTTIDELTPLIGKKVIVKDKEVGLFLTESGKIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            5536..6513
                     /gene="cobA"
                     /locus_tag="NBK22_000811"
     CDS             5536..6513
                     /gene="cobA"
                     /locus_tag="NBK22_000811"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCD2414014.1"
                     /translation="MSVEEYGKVYLIGAGPGNPNYLTKKAERLIREADVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNEHHIAFEIVPGVTSASAAVATMQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGRLSNII
                     SKLQHYSIEAKPGICIIGEVVGYTENTPKSYDSMKQFYVVSGSKHDALMLCEHLYDEG
                     YGCLLNPNDTSNGTYHSSQYDYYDAFIKQQENVTYISTDRADANTVLCH"
     gene            6822..10511
                     /locus_tag="NBK22_000812"
     CDS             6822..10511
                     /locus_tag="NBK22_000812"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCD2414015.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTEIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFIIMLDEDENGYKAGRFLRASDLGQTTEQGEWKPVIHDAISDSLVVPNGTMGQRWE
                     DGKKWNLKLETEDGSKINPTLSMTDGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEAISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIESNEAILKRAIDEVKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNDDSVRANRPRIDTARNVADAILSISSATNGKLSQKSYEDLEEQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNIERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGNRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            10501..12060
                     /gene="narH"
                     /locus_tag="NBK22_000813"
     CDS             10501..12060
                     /gene="narH"
                     /locus_tag="NBK22_000813"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCD2414016.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGIPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            12053..12628
                     /gene="narJ"
                     /locus_tag="NBK22_000814"
     CDS             12053..12628
                     /gene="narJ"
                     /locus_tag="NBK22_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCD2414017.1"
                     /translation="MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPA
                     YEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            12621..13298
                     /gene="narI"
                     /locus_tag="NBK22_000815"
     CDS             12621..13298
                     /gene="narI"
                     /locus_tag="NBK22_000815"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCD2414018.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKPWMDAVGVSEQLYHIGAVYIGSIFGIITLI
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKMHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            13318..13770
                     /gene="nreA"
                     /locus_tag="NBK22_000816"
     CDS             13318..13770
                     /gene="nreA"
                     /locus_tag="NBK22_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCD2414019.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            13794..14828
                     /gene="nreB"
                     /locus_tag="NBK22_000817"
     CDS             13794..14828
                     /gene="nreB"
                     /locus_tag="NBK22_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCD2414020.1"
                     /translation="MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            14851..15504
                     /gene="nreC"
                     /locus_tag="NBK22_000818"
     CDS             14851..15504
                     /gene="nreC"
                     /locus_tag="NBK22_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCD2414021.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(15762..16502)
                     /locus_tag="NBK22_000819"
     CDS             complement(15762..16502)
                     /locus_tag="NBK22_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD2414022.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGHSLIDTSILNKTLQSQYDKEASIKYGHTEAYQSFIRRKDSLQSQDIRHKLTT
                     IFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(16481..17395)
                     /locus_tag="NBK22_000820"
     CDS             complement(16481..17395)
                     /locus_tag="NBK22_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCD2414023.1"
                     /translation="MYKINIIRSDSVYKNILKAPINDRDSIFTKEILVPFKKKFEVQH
                     MPIYNDDKQTMSAIQFLDAFQKSPKELVETDQMSIQYLNNDFWSNCEKYLKVAIDQFS
                     NYSISSQVSNYHFTVLLGDSQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTNWSRDNV
                     KIKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYACGYHLVKYFLQKT
                     NIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            17764..18933
                     /locus_tag="NBK22_000821"
     CDS             17764..18933
                     /locus_tag="NBK22_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCD2414024.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VVMIIGAIILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFLNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKNG"
     gene            complement(19040..19468)
                     /locus_tag="NBK22_000822"
     CDS             complement(19040..19468)
                     /locus_tag="NBK22_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCD2414025.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRSAKYKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNETTSSTSIPIS"
     gene            complement(19684..20130)
                     /locus_tag="NBK22_000823"
     CDS             complement(19684..20130)
                     /locus_tag="NBK22_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2414026.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIKKYTNYLKEYDLTYTGYIVL
                     MAIENDEKLNIKKLGERVFLDSGTLTPLLKKLEKKDYVVRTREEKDERNLQISLTEQG
                     KAIKSPLAEISVKVFNEFNISEREASDIINNLRNFVSKNFDYSDKK"
     gene            20528..20893
                     /locus_tag="NBK22_000824"
     CDS             20528..20893
                     /locus_tag="NBK22_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCD2414027.1"
                     /translation="MKKKKNAIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKNLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            21075..22352
                     /locus_tag="NBK22_000825"
     CDS             21075..22352
                     /locus_tag="NBK22_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCD2414028.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLSAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(22623..22979)
                     /locus_tag="NBK22_000826"
     CDS             complement(22623..22979)
                     /locus_tag="NBK22_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCD2414029.1"
                     /translation="MKKKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     STIEKMISEKDESIEKNVKLPSDSDVKVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
BASE COUNT         7965 a         3352 c         4500 g         7389 t
ORIGIN      
        1 gagataaaat gagcgtcgta atatcttaaa tcataaagat attagccaat cacgcgattt
       61 atctcgctgt ttttatgaaa gaaaactaca ttttcgagag aataaaaaga tttttctgaa
      121 atttaatcta tcaattacat tgatttacta atataattag aaagtaaatt ttatttgatc
      181 aaagtaaatg ggggaagtac tgtgatagaa aataagaaaa cagttgaaga tacatattca
      241 acaggcgcaa ttgttgattc aatatcatct tcagtacaaa tgaagcaagt aatggtacag
      301 caaacacctg gccgatatat gcttaaagca atgatggccg gatttttact ctcaatcgtt
      361 actgtattta tgttcggaat aaagacgcag tttgcgagta ctcacgtaga tggacttatc
      421 aatttaatgg gagccattgc gtttagttta ggtttgattt tagttgtatt aactaactct
      481 gaattattaa caagtaactt catgtacttt acagtaggct ggtattataa agtcgtttct
      541 gttaagaaga tgacttggat tttattatat tgtttcttag gaaatatttt aggtgggttt
      601 gtgttatttt tcttaatgaa atttgcgcat gtcatgacac ctgaaatgac acaagcttta
      661 acagctttag ttcagaaaaa gactgttgat tcaacatggt tgaatatttt tacaaaaggt
      721 attttctgta acttctttat taatattggt attttcatct ctatgcaatt caaaggcggc
      781 ttaacaaagg cattcttcat agcttgtggt gtcgtggtct ttgtatacat gggatatgag
      841 cacgttgtgt tcaatgcagg tctatatgca ggtatggtat tctttaacct tgatgcagtt
      901 tcatggttac acgttttaaa aaatatcgtt tttgctttct taggtaactt tgtcggtggc
      961 ggtatttttg taggtctcgt ttatgctttc ttaaatggta agcgtaatcg tttagagcaa
     1021 caataattaa gatgtagtga ttaaatagcg taacaatgaa tcattcttcc aaaccattca
     1081 tgaatttgaa tggtttttta cgttgagaga gtaattttta ataagtttca tgagagtatt
     1141 ggtgtattca gatgaagcat actttctgac atgtgataaa tttattgata cactttgatg
     1201 gatatttatt ttaaaaataa aatatggtta aatcatttaa aaaagagtgg ggattaatat
     1261 gacaaaaacg atgattcgac tcgcaagtga acaagatgca gaaaaattgc aacaactcat
     1321 gcatgaagca tttacgcctc taagagaact aggtattgat tggccttcgg ttaatgctaa
     1381 tttagatgct gttaaagaaa atatagataa aaatactaca tttgtcatga caattgatga
     1441 tgaaattata tctacgatta cggttagata tccttgggga agtgtgaaaa gtatttcggg
     1501 ttatcctttt gtctggtggt ttgcgacaaa tcctgagtat gaagggaaag gctatggtag
     1561 ccaattactt acttatgttg aagaagcatt tttgcgagat acgttaaagt cagcggcagt
     1621 tactttaggt acatctgcaa gattgcatcc gtggttactt aaaatttatg aaaaaagagg
     1681 ttatgaaata tacagcgaac atgaaaatga tgatggcgat ttaggtgtga tcatgcgcaa
     1741 agtattgata cccgagcgct ttgacgaaac aatattaggc caaccaccat tttaagataa
     1801 taatatacac tacaagcgac cgatatatta atgcttgctc aaataaatgt tgagtaggga
     1861 ttcatatatc ggtcgctttt tttgatttaa caaaaaggga aaatgtataa ggaatatgca
     1921 tgactacagg catcagggaa ttccctgatt ttacattata atgtgattct tttcacaaaa
     1981 acaaaatgat ttggtataca atacgtgtga aagggttggt aaatgtgaat gggaatatca
     2041 ttgttgcaca tggcatgagg cacggacgac agaatcaagc gttagaagca tttatttctg
     2101 aattagtaaa agatgatata catcactatg atattgcgtt tttagaaagt gagcatcagg
     2161 atttagagac ggtaatgacg acgttgattc aaagtggggt tgaccatttt aaaattgtac
     2221 cattgctaat ctttagtgca atgcattatc tcaaggatat accgaatatc gttcatgaaa
     2281 tgaaacgacg gtatccagac ataaaggtag aagtaagcga gccacttggc acacatcctt
     2341 taatgagacg attaattgaa caacgtattt gtgacgcatt agcaggtgac gaacaacaag
     2401 ttggagttat ggttgttgca cacggcaata ttaatggaaa gtttactaag gcacacgaag
     2461 agttacaaat atgcgctcaa aatttgcaaa ttagcaagcc gacatatgcg agaacgctat
     2521 atggagagat tagttttaca catgatttag aaagcatctc gaaacggtat caaaaattga
     2581 ttgtcgtacc attattttta tatgacggaa gacttgttaa caaagtgaag cagcaaatga
     2641 atgacatggt tattaataca gatatacact tcacaccttc aattaacttc gacccaattt
     2701 taaagcaaat cattaacgat agattagaaa gcttaatgat tccaatgaaa atataaaagc
     2761 actaacgaaa aattaatacc tttttttgaa ataaatatta tggagtaaag gggcatattt
     2821 tatggcaaag caaaaactag taatgattgg taacggtatg gcgggcattc gaacaatcga
     2881 agaaatatta gagcgcgcca acgatttata tgatattacg gtaataggta aagaacctta
     2941 tccaaactat aaccgcatca tgctttcaaa tattttacaa aataaaatga cagttgaaga
     3001 aacaattatg aatccatatg agtggtatga ggaacatggt atcgagttaa ttacaaatga
     3061 tcctgtaatt gaagtagata gagcaaatca atcagttact actgcaaatg gtattgaagt
     3121 atcatacgat aaattgattt ttgcgacagg ttctaaagca ttcgtcattc cagttccagg
     3181 ttcgacatta ccgagtgtaa ttggttggag aacgattgat gatactgaac aaatgatgaa
     3241 cattgctaag actaaaaaga aagcaattgt aattggtgga ggattactag gtttagagtg
     3301 tgcacgtggt ttattagatc aaggtatgga agtaacggtg ttacatttag ctgaatggtt
     3361 gatggaaatg caactagacc gtaaagctgg aaatatgctt aaagcagatc tagaaaagca
     3421 aggtatgaag tttgaaatgc aagccaacac aactgaaatc ttaggagaag atgatgttga
     3481 gggtgttaaa ttagctgatg gacgcgagat tccggcagac ttagttgtta tggcagtagg
     3541 tatacgacca tacacagaag tagccaaaga atcaggtcta gatgttaatc gtggtattgt
     3601 tgtcaatgat gtgatgcaaa caagtgatag caatgtatat gcagttggtg aatgtgcaga
     3661 acataacggc aaagtttatg gactcgttgc accactatat gaacaaggta aagtattagc
     3721 tgatcattta acaaataaag aaacgaacgg atacaaggga tcaacaacat ttacgtcatt
     3781 aaaagtttct gggtgtgact tgtatagtgc tggtcaaatt gtagaaaatg cagaaattaa
     3841 aggtattgaa atatttaata gtgttgataa taactataaa aaaatctttt taaaagacgg
     3901 taatgtagtt ggtgcagtac tatatggtga tatcgatgat ggttcacgct tttataaaat
     3961 gatgaaaaaa ggtgaatcca ctgaagatta cacacttgta tcattgctta ctaaaggtgg
     4021 agaagaggca tcgctatcaa ttgctgatat ggctgatgat gaaacaattt gtggttgtaa
     4081 tggtgttgat aaaggtacta tagtaaatgc gattacggaa aatggcttta caacagttga
     4141 agaagtaacg gctaaaacaa aagcggggaa ttcatgtggt aaatgtaaac cgcaaattgc
     4201 tcaaatattg cagcacactt taggagatga ctttgttgcc gcaaaacctg ctggtatatg
     4261 tggttgtact gatttgacac gcgatcaaat agtaacgcaa ataagagcga aaggtttaaa
     4321 aacatctaaa gaagttcgac atgttttaaa ctttaaaaac aaaggtggat gtccaaaatg
     4381 tcgacctgcg attaactatt atttgaacat ggtttatcca catgatcatg aagatgaaag
     4441 agaatcaaga tttgctaacg aacgttacca tgcgaatatt caaaatgatg gtacattttc
     4501 tgttatacct caaatgcgtg ggggtgttac agatgcagac caactgattc gtctaggaga
     4561 agtggctaag aaatatcatg tgccactagt taaagtgaca ggttcacaac gtgttggttt
     4621 gtatggagtt aaaaaagaag aattaccaaa tatatgggaa gacctaggta tgcgttcagc
     4681 atcagcttat ggtaagaaaa cacgctcagt taaaagctgt gttggtaaag agttttgtcg
     4741 atttggtacg caatacacga cacgacttgg cattcgttta gaaaaaacat ttgaatacat
     4801 cgatacacct cataaattca aaatgggtgt atcaggctgc ccaagaagtt gtgttgaatc
     4861 aggtgttaaa gattttggta ttatctcagt tgaaaatgga ttccaaatct atatcggtgg
     4921 taatggtggt acagaagttg aaaaagctga atttttaaca actgtagaaa cagaagatga
     4981 agtaatcaaa ttatgtggtg ctttgatgca atattatcgc gaaacaggta tatatgctga
     5041 aagaacagcg ccatggttaa gaagacttgg atttgaaaat gtaaaagaag tcttacttga
     5101 cccagaaaga caaaatgaac tatttgaacg cattatggat gctaaaaaag cagtcgaagc
     5161 tgaaccttgg gaagcaataa ctagcaatgc gcaagcacgt aaaatttttg aagtggagaa
     5221 ggtgtaatac atggaaacaa aagaaaaaat taaagtgaca actatagatg aattaacacc
     5281 cctaattgga aaaaaggtta ttgtcaaaga caaagaggta ggattgtttt taacagaaag
     5341 tggcaaaatt catgcgattc acaatatctg tccacacaaa caaggaccat tgtctgaagg
     5401 gacagtgagt ggggaatatg tattttgccc gctccacgat caaaaaattg atttaaacac
     5461 aggtattgtt caagaacctg atgaaggttg cgtagatgtt tatgaggtag aagttacaga
     5521 cgggaacgta tatatatgtc tgtagaggaa tatggcaagg tttacttgat aggtgcgggc
     5581 ccaggtaatc cgaattattt gactaaaaag gctgagcgtt taatacgtga agcggatgtc
     5641 attctatatg atcgattagt gaatccgtta attttacaat atgcgaattt gactactgaa
     5701 attatcgatg ttggtaaaaa gccgtatgca aaacatattc agcaagaaaa aataaatgac
     5761 tgtatcgtgg aagcagcgcg tcgatataac aaggttgtta gacttaaagg tggcgatcca
     5821 gcgatatttg gtcgtgtgca agaagaagtc gacacactaa atgaacatca tattgcgttt
     5881 gaaattgtac caggggtgac atcagcgagc gcagcagttg ctactatgca gacaggtttg
     5941 acaatgcgta cagttgctaa aagtgtgaca ttttctacag gtcactttaa agattcagaa
     6001 gaaaatgaag tggatgtcaa ttccttagtg aatggtggca cgttagcaat ttatatgggg
     6061 gtaaaacgtt tagggaaaat tatcacacag atacaacaat acacagatat tgattacccg
     6121 atagcgattg tctttcaagc atcgtgtttt aacgagtttg ttgttaaggg gcgtttaagt
     6181 aatattattt caaaattgca acactattca attgaggcga aaccaggtat atgtattatt
     6241 ggggaagttg ttggttatac tgaaaacact cctaaatcat atgattctat gaagcaattt
     6301 tatgtagtaa gtggttctaa acatgacgcc cttatgctct gtgaacattt atatgacgaa
     6361 ggttatggct gtttgctaaa cccaaatgat acatcgaatg gcacatatca ttcgtcgcaa
     6421 tatgattact atgatgcatt tattaagcag caagaaaatg taacatatat ttcaaccgat
     6481 cgtgcagatg ctaatacagt gttatgtcac taatttataa aaaataaatg aataagtaag
     6541 gtttcaaccg agagaatata ttcgtgttga agccttattt gtcgttgtgc caaatttgaa
     6601 cgatttagat tggcaataag catgaccata catgattgtg tattatttca atgaaatccc
     6661 gttattgata gggaaattcc ttaagtaatt aggtgattcc ctaatatttt atgtttgtga
     6721 aaaaatgtac aatctaatta aagcaatagt cttgggcatt ttaaagtatt taaaaacacg
     6781 acatctacac atcgtatttt tatacgtagg aggatataaa tatgggaaaa tttggattga
     6841 atttctttaa gccaacagaa aaatttaatg ggaattggtc gatcctagaa agtaaaagta
     6901 gagaatggga aaaaatgtac agagaacgtt ggagccacga taaagaagta agaacaacac
     6961 atggtgttaa ctgtacaggc tcatgttctt ggaaagtatt tgtgaaaaat ggtgtgatta
     7021 cctgggaaaa tcaacaaact gactatccaa gttgtggtcc ggatatgcct gaatacgaac
     7081 cgagaggatg tccacgaggt gcgtcattct cttggtatga atacagtccg cttcgaatca
     7141 aatatccata tattcgtgga aaactctggg atttatggac tgaagcatta gaagaaaaca
     7201 atggtaatcg cgttgctgca tgggcgtcta ttgttgaaaa tgaagacaaa gccaaacaat
     7261 ataagcaagc ccgaggtatg ggagggcacg tgcgttcaaa ttggaaagac gttacagaga
     7321 taatcgcagc acaattactg tatacaataa aaaaatatgg tccagatcga atcgcaggat
     7381 ttacacctat tccagcgatg tcaatgatta gttatgcagc aggtgctcga ttcatcaatt
     7441 tgcttggtgg tgaaatgctt agtttttatg actggtatgc agatttacca cctgcctctc
     7501 cacaaatttg gggagagcaa acagatgtgc ctgaatcaag cgactggtat aacgcatcat
     7561 acattattat gtgggggtct aatgtacctt taacacgtac tccggatgca cattttatga
     7621 ctgaagtccg ctataaaggt acaaaagtca tttcagtagc accagattac gcagaaaatg
     7681 tgaaatttgc agataactgg ctagcaccga atcctggttc agatgctgca attgcacaag
     7741 caatgacgca tgttatttta caagaacatt atgttaatca acctaatgaa cgctttataa
     7801 attacgccaa acaatataca gatatgccgt ttattatcat gctggatgaa gatgaaaacg
     7861 gatataaagc gggtcgattt ttaagagcga gtgacttagg tcaaacaaca gagcaaggcg
     7921 aatggaagcc agttattcat gatgcaatca gcgatagttt agtagtacct aatggcacaa
     7981 tgggtcaacg ttgggaagat ggtaagaagt ggaacttaaa actagaaaca gaagatggtt
     8041 ctaaaattaa ccctacacta tcaatgacag atggtggata cgaattagaa acaattcaat
     8101 tcccatactt tgatagtgat ggagatggga tattcaatcg tccaattcca actcgacaag
     8161 tcactttagc aaatggtgac aaagtccgta ttgctacaat ttttgactta atggcaagtc
     8221 aatatggcgt gcgtcgtttt gatcataaat tagaatcaaa aggatacgac gatgcagaat
     8281 caaaatatac acctgcttgg caagaagcca tttcaggcgt aaaacaaagt gttgtcattc
     8341 aagtagcgaa agaatttgcg caaaacgcta tcgacactga agggcgttca atgattatca
     8401 tgggtgcggg tattaaccat tggtttaact cagatacgat ttatcgttca atcttaaact
     8461 tagttatgtt atgtggctgt caaggtgtga atggtggcgg ttgggctcac tatgtgggac
     8521 aagaaaaatg tcgtccgatt gaaggatgga gtactgtcgc atttgcgaaa gactggcaag
     8581 gaccaccacg tttgcaaaac ggaacaagtt ggttctattt tgcaacagac caatggaaat
     8641 atgaagagtc aaatgtagat cgtttaaaat ctccattagc taaaacagag gagttaaagc
     8701 atcaacatcc agctgattat aatgttttag cagctagact tggttggtta ccatcttatc
     8761 cacaatttaa taaaaatagt ttgttgtttg cagaagaagc taaagatgaa ggcattgagt
     8821 cgaatgaggc aattttaaaa cgcgcgatag atgaagttaa gtcaaaacaa acgcaatttg
     8881 cgatagaaga tccggatttg aaaaagaatc atccgaaatc actgtttata tggcgctcaa
     8941 atctaatctc aagttctgca aaaggtcaag aatactttat gaagcattta cttggcacaa
     9001 aatcaggttt attagctaca ccaaatgaag atgaaaagcc agaagaaatt acgtggcgtg
     9061 aggaaacaac agggaaatta gatttagtcg tttctttaga tttcagaatg acagcaacac
     9121 ctttatattc tgacattgta ttgccagcag cgacttggta tgagaagcat gatttatcat
     9181 ctacagatat gcatccatat gtacatcctt ttaatccagc tattgatcca ttatgggaat
     9241 cgcgttcaga ctgggatatt tataaaacgt tggcaaaagc attttcagaa atggcaaaag
     9301 actatttacc tggaacgttt aaagatgttg tgacaactcc gcttagtcat gatacaaagc
     9361 aagaaatttc aacaccatac ggcgtagtga aagattggtc gaagggtgaa attgaagcgg
     9421 tacctggacg tacaatgcct aactttgcaa ttgtagaacg cgactacact aaaatttacg
     9481 acaaatatgt cacgcttggt cctgtacttg aaaaagggaa agttggagca catggtgtaa
     9541 gtttcggtgt cagtgaacaa tatgaagaat taaaaagtat gttaggtacg tggagtgata
     9601 caaatgatga ttctgtgaga gcgaatcgtc cacgtattga tacagcacgt aatgtagctg
     9661 atgcaatact aagtatttca tctgctacga atggtaaatt atcacaaaaa tcatatgaag
     9721 atctcgaaga acaaactgga atgccgttaa aagatatttc tagcgaacgt gcagctgaga
     9781 aaatttcgtt tttaaatata acttcacaac cacgagaagt aataccgaca gcagtattcc
     9841 caggttcaaa taaacaaggt cgacgatatt caccatttac aacgaatata gaacgtctag
     9901 taccttttag aacattaaca ggacgtcaaa gttattatgt ggatcacgaa gttttccaac
     9961 aatttgggga gagcttacca gtatataaac cgacattgcc gccaatggta tttgggaata
    10021 gagataagaa aattaaaggt ggtacagatg ctttggtact gcgttattta acgcctcatg
    10081 gaaaatggaa tatacactca atgtatcaag ataataagca tatgttgaca ctatttagag
    10141 gtggtccaac ggtttggata tcaaatgaag atgctgaaaa acacgatatc caagataatg
    10201 attggctaga agtgtataac cgtaatggtg ttgtaacggc aagagcagtt atttcgcatc
    10261 gtatgcctaa aggtacaatg tttatgtatc atgcacaaga taaacatatt caaacgcctg
    10321 ggtcagaaat tacagataca cgtggtggtt cacacaacgc gccgactaga atccatttga
    10381 aaccaacaca actagtcgga ggatacgcac aaattagtta tcactttaat tattatggac
    10441 caattgggaa ccaaagggat ttatatgtag cagttagaaa gatgaaggag gttaattggc
    10501 ttgaagatta aagcgcaagt tgcgatggta ttaaatttag ataaatgcat aggatgccat
    10561 acgtgtagtg tgacatgtaa aaacacttgg acaaatcgtc caggtgctga gtacatgtgg
    10621 ttcaacaacg tagaaacgaa gccaggtgta gggtatccga aacgttggga agaccaagaa
    10681 cactacaaag gtggttgggt actaaatcgt aaagggaaac ttgaattaaa atctggaagt
    10741 agaatttcaa aaattgcttt aggtaaaatt ttttataacc cagatatgcc attaataaaa
    10801 gattattatg aaccatggaa ctataattat gaacatttaa caactgcgaa atcagggaag
    10861 cattcgccag ttgctagagc gtattcagaa attacagggg ataacattga aattgaatgg
    10921 ggacctaact gggaagatga cttagcaggt ggtcatgtta caggtccaaa agatcctaac
    10981 atccaaaaaa tagaagaaga tattaaattc caatttgacg aaacttttat gatgtattta
    11041 ccacgtttat gtgaacattg cttgaatcca agttgcgttg catcgtgtcc ttcaggtgct
    11101 atgtataaac gtgatgaaga tggcattgta ttagttgacc aagacgcatg tcgtggatgg
    11161 cgttactgta tgacaggttg tccatataag aaagtttact tcaattggaa aacgaacaaa
    11221 gctgaaaagt gtactttctg tttcccgaga atcgaggcgg gtatacctac ggtatgctct
    11281 gaaacttgta caggacgtat gcgttactta ggtgttttat tatatgatgc agatcgagtt
    11341 catgaagcag cttcagcagt agacgagaaa gatttatatg aaaaacaatt agatatattc
    11401 ttaaacccat ttgacgaaga agtcattgct caagctgaaa aagatggtat tggttacgat
    11461 tggattgaag ctgcacaaaa ctctccaatt tataagttag caattgaata taaacttgca
    11521 tttccgttac atcctgaatt tagaacgatg ccgatggttt ggtattgccc accacttagc
    11581 ccaattatga gctattttga aggtaaaaat acaactcaaa atccagatgc gattttccca
    11641 gccattgaag aaatgcgttt acctattgaa tacttagcta atattttcac tgcaggagat
    11701 acagagccag taaaaggagc cctacaacgt atggcaatga tgagaagtta tatgagatct
    11761 caagttactc aacagccatt tgacacgtct cgattagagc gattaggtat aaccgagcgc
    11821 caaactaaag acatgtatcg tttattagga ctagctaaat atgaagatcg atttgttatt
    11881 ccaacatcac acaaagaaac ctatttagat acgtatcacg cacaaggaag tacaggatac
    11941 aattacggcg gcgagcattt tggagataac tgtgaaggct gtggtgttgc agtaggttca
    12001 gggaaaactg gtcaagaaat ttataatgag aatttctatg gagggatttt ccgtgattaa
    12061 tttcgataat tttaaaaaat atcaggaaag ttttggatat atggcacaac agttatgttt
    12121 tccagaaaaa ttaacttttc atccaaaaac ttttgaagaa acaatttcga aagatcatcc
    12181 agcatatgaa gatttagtag catttagaaa tgtaatgatg acattttcat tatcagaaat
    12241 caaagcgatt tatacagata catttgattt tacgaaaaaa gcaccattat atatgacgta
    12301 taacaaattt gatacgcaaa aagaacgtgg tcaaatgtta gctaaattga aggtattata
    12361 tgaaatgttt ggactagaaa tggtagataa tgaactttct gattatctac cgttgatgct
    12421 tcaatttttg caagtggcag aatggcgaaa tgatccaaga gctgaagaga atattcaatt
    12481 aatcattatg attattgagg atggaacata tgtgatggct aatgcacttg gtgaagataa
    12541 caatccatat gcctatgtga ttcaagcact tagaaaaaca ttgaaattat gtttgacatc
    12601 accaaaagga gtgaaacatc atgcttaatc agtttttatg ggttatattt ccgtatttat
    12661 gtttagttgt ctttgtcgca ggtcatattg cacgttatcg ttatgataaa ttttcttgga
    12721 cagctaaatc tagtgaatta atagaaagaa aaaggttgat gtggggaagt ttattatttc
    12781 acttaggtat tattccagtg ttttttggac acgtagttgg attgttaata ccgaaaccat
    12841 ggatggatgc agtgggggta tctgagcaat tatatcatat aggcgctgtt tatattggta
    12901 gcatctttgg tataataact ttgataggga tgtttttatt aacagcgaga cgtgttacta
    12961 cgaaaagtat tcgaagatta agttcagcgt cggatatatt tgtaaatttc ttattattac
    13021 ttatcgtatt tatgggatgt tacgcaacat tagtaaccaa tatacaacat ccagatttta
    13081 actacagaac ttcattatca atttggttta gacaattatt tatgttaaaa ccagatgcgt
    13141 cattgatgag tggtgtgccg attgcattta aaatgcatat attgttaggt ttcactatta
    13201 tggcttgttg gccatttaca cgtttggtgc atgtttggag tgtacctttg acatatataa
    13261 atcgcagata tattatttac cgtaagaata aagtgtaagc ataagaggtg aaaggatttg
    13321 acaccagaag caataattga agatcgtaga tttcaagaga cattagataa aatacgcaaa
    13381 gaagagggtt atgattttgc ggcaatcgct ttctatgaat ctaacaaacc atcatctcca
    13441 ataaaatggc actatgtttc aggtaataaa aataatcgat ttaaattgat tattttaaga
    13501 aaaggtcgtg gattagctgg aactgttatg aaaacaggaa agcgtatggt aatagccaat
    13561 gttggtttag ctttagggcc tgaagaaaag attgactatc caattttatt gagcgaatca
    13621 ttaacagcag ttttagcagt gccgctttgg tataagaatc aagtgtatgg cgtactatta
    13681 tttggtcaaa gagatggtcg tcctttacca aaaatatttg ataatgatga tattcaacgt
    13741 aagtttggta ttttcaatga tgataaataa tgagatgaat tagggtgtaa gtcatgatta
    13801 atgaggacag tatacagtta gatactttat tgaaaaaata ttatgagcat tcaatcgaaa
    13861 agatagtttt tgctgatgat aacggtaaaa ttattgcaat gaatgatgct gctaaagata
    13921 tattatctga agaagataat tacagtgctg tggcgaatgc gatttgtcac agatgtgaag
    13981 ggtatacaaa tgcctatgat gtgcaatcat gtaaagactg tttcttagaa tcgatgcaag
    14041 tacaagctac gaatttccaa gtatttatga agacgaaaga tcaaaaagtg atgccattta
    14101 cagcaacata tcagttgatt gatcaagaca ggggaattca tgcatttacg cttcaaaatg
    14161 tttcatcaca aattgaacag caagagaaat tacatcaaca acgtatgatg cgtaaaacaa
    14221 tctcagccca agaaaatgaa agaaagcgaa tttctagaga gttacatgat agtgtgattc
    14281 aagaaatgct caatgtagat gttcaattga gattattaaa atatcaagaa gatacaacta
    14341 agcttttgga agacgcagaa aatattgaat atattgtcgc taaattaatt gatgatatac
    14401 gtaatatgtc ggttgaatta agacccgctt ctttggatga tctaggcctt gaagcagctt
    14461 ttaaatcata ttttaaacag tttgaagaaa attatggaat aaaaatcata tatacgtcaa
    14521 atattaaaaa tactcgcttt gatagtgata ttgaaacggt tgtttatcga gtagtgcaag
    14581 aagctatttt aaatgcgttg aaatacgctg atgtaaatga aataaatgta ggtatccgtc
    14641 aaactgggcg ccatttggta gcagaagtga tagatgcggg aaatggtttt gatccaagtt
    14701 ctaagcctaa aggttctgga ctcggtttat atggaatgaa tgagcgagct gagttagtca
    14761 gcggtagcgt caatattgaa acgaaaattg gtgagggtac caatgttaca ttgaacattc
    14821 caatttgaaa catacattgg gggaataaaa ttgaaaatag tcattgccga tgatcacgct
    14881 gttgtccgta cggggttctc tatgatttta aattatcaaa atgatatgga agttgttgca
    14941 acggctgcag atggcgtcga agcttaccaa aaagtaatgg aatataaacc tgatgtgtta
    15001 ctaatggatt taagtatgcc accaggtgag tcaggtctta tcgctacgag taaaattgct
    15061 gacagttttc ctgaaactaa aatactaata ttaacaatgt ttgatgatga ggagtatttg
    15121 ttccatgtgt tgcgtaatgg tgcgaaaggt tacatattga aaaatgcacc tgatgaacaa
    15181 ttattgttag ctattcgaac tgtatataaa ggtgaaacat atgtagatat gaaacttaca
    15241 acatctttag tgaatgaatt tgtatctaat tcaaatcagg acactgcaaa cacaacagat
    15301 ccttttaaaa tcttatcaaa acgagaacta gaaatattgc cacttattgc caaaggttac
    15361 gggaataaag aaattgcaga gaaattattt gtatctgtga aaacagtaga agcacataag
    15421 acgcatatta tgacaaagct tggcttaaag agtaaacctg agctagttga atatgcattg
    15481 aaaaagaaat tattagagtt ttagagtttg aaattaatat aattcagtat aaaagcgcga
    15541 gaggaaatga ttatcttctg gcgcttttgt atgtttcgaa atatattttt ttataaatcg
    15601 aatggaacct aatcctttct cctttcggta acctatacta attcgctttg caaaggtata
    15661 acttttaata tagttattga tttagagaat aaatgattag ttttctactt tgaacaatta
    15721 ttttagttgg tgtattcagt gttgtagcat ctgtatgagg attaaaaatt gtcgctctta
    15781 ttcacattgc tcaaaaaata attaacagct tctgaaatgt atgatgctaa attttgatta
    15841 tcatatgaat taaagtaatc tttgaaacgc gtatcatctt catatgtttt agcaatacag
    15901 cataatgttt catcatcaaa atcagcgata gtgttcataa atgccttcca ctcaaatacg
    15961 agatcactac aatcttggat tggataatga ctcaaagaca tatgattaaa tttattgaag
    16021 atagttgtca atttatgtct gatatcctgc gattgtaagc tgtctttacg tctaataaat
    16081 gattgatatg cttccgtatg accatatttt atagatgctt ctttatcata ttgtgactgt
    16141 agtgtcttgt ttaaaatgct ggtatctatt agagagtgtc ctttaataaa ttcacataca
    16201 gagcgtctaa ttaattgtaa gtcagaaatc tttttatcta acttgcttat ttgctctgat
    16261 aataactttc gcaattgttc attatcatac gaaatgtatt gttttatttt agcgatatca
    16321 aaatcaaaag acttgagtat taaaatcttt tgtaattttt ctaagtcttg ctgtttataa
    16381 acgcgataat ttttatcatt tttatctgga actaataatc ctatttcatc gtaataatgt
    16441 aaagttcttt tagttacacc tgtaatttca ataatatctt ttaaagtgta tgtgtatgcc
    16501 aaaattcagt cacctcattt attatttttt gtgctggaag tgttgtggca acttcaatag
    16561 gtatgtttgt tttttgtaaa aagtatttta ccaagtgata gccacatgca tacccggcag
    16621 catgagatag gccaacgata ggcctacctt gaagtttatt aatatcatca ccatagagat
    16681 aaggcatcga ttcaaatata tgctttaaat gtaaatgatt ataaatagta tttttaattt
    16741 ttacgttatc acgactccaa ttagtatttg ttacccacgg tccaatatgc gctttaccat
    16801 acaatgattc tacatagttt tcagctaatc cttctgcaat aatcaattca atcaaacttc
    16861 cgccatccca atcaatatat tgatagcgca tattatgatt tacttcatgt gcaattaagc
    16921 ttttcataga attaattgta cttgtactcg gcactaaata aatcataata taacctggta
    16981 tgccaccatc accaccgcga tttttattta gatacataag tggtttttgg ctatctccaa
    17041 gtaatactgt gaaatgatag ttagaaactt gagatgagat actataattt gaaaattgat
    17101 ctatagcaac ttttaaatat ttttcgcaat tactccagaa atcattattc aggtattgta
    17161 ttgacatttg gtcagtttca accagttctt tgggagactt ttgaaatgca tctaaaaatt
    17221 gaatcgcact cattgtttgt ttgtcatcat tataaatagg catatgttgt acctcaaatt
    17281 tctttttaaa aggtactaat atttctttag tgaaaattga atcacggtcg tttattggtg
    17341 ccttgagtat attcttatag acggaatctg atctaataat attgatttta tacatgataa
    17401 acctccttat gttgtcagca taaaggataa cgtaacgtga ttttcaagca gtaattgtaa
    17461 ctaattgaaa aaaataagaa aagtatgtga gtgttcctaa ataatatgat taaaatgatg
    17521 gcgaataagt gtctaaaagc atcttaaagg gacattgtat agggtaaatc acttcataaa
    17581 taagggaaaa tccttatgtt cactttttca caatcataat aaaatatata tgtagtcaat
    17641 actttgtcta tattgaatgt tttcatataa atgaaagcat ttttaaataa cattgacctc
    17701 taatatatag gcagagtatt gatatctatt aaaaaataaa tgattttggt gaaggtgaaa
    17761 cgtatgtaca aaacaaaagg tggctttcaa cttacattac agacattaag tttagtggtt
    17821 gggtttatgg cttggagtat aattgcgcca ttaatgccct ttattaaaca agatgtcaat
    17881 gttactgaag gtcaaatatc aatcatttta gcgataccag ttattttggg atcggtgctc
    17941 cgtgtgccat ttggttatct aacaaacatt gttggcgcta aatgggtatt ctttactagt
    18001 tttatcgtat tgttattccc gatatttttc ttaagccaag cacaaacacc gggtatgtta
    18061 atggcgtcag gatttttcct tggtgtaggt ggtgcaattt tctcagttgg tgttacatca
    18121 gttcctaaat atttccctaa agaaaaagta ggtctagcaa atggtattta tggtatggga
    18181 aatatcggta cagcagtttc ttcattttta gcaccgccaa ttgcgggtat tattggttgg
    18241 caaacaactg ttagaagtta cttaattatt atcgctttat ttgcattaat tatgtttata
    18301 tttggtgata cacaagaacg taaaattaaa gtgccattaa tggcacaaat gaaaacatta
    18361 tctaaaaact acaaattata ttacttaagt tattggtatt tcattacttt tggtgctttt
    18421 gtagcatttg gtattttctt acctaactac ttagtaaatc attttggaat tgacaaagta
    18481 gatgctggta ttcgatcagg tgtattcatt gcgctggcaa cattcttaag accaataggt
    18541 ggcattttag gtgataaatt taatgcagtt aaagtattga tgattgattt tgttgttatg
    18601 attatcggtg ccattatttt aggtatttca gaccatatcg cattattcac tgtaggctgt
    18661 ttaacaataa gtatttgtgc aggtattggt aacggcttaa tcttcaaatt agtaccatcc
    18721 tacttcttaa atgaagcggg atccgcaaat ggtatcgtat caatgatggg tggtttagga
    18781 ggattcttcc caccactagt aatcacgtac gtagctaatt taacaggatc aagtcattta
    18841 gcatttattt tcttagcggt atttggatgt attgcattat ttaccatgcg tcatttatac
    18901 caaaaagaat atggctcatt gaaaaacggt tgatatgtaa tacatgacat tcatttagtt
    18961 aaatacaaag ccttaatatc atgcgcaata ttcgtagcat gacattaagg ctttagtagt
    19021 gtattcattt taaatttaac tatgaaattg gaatagatgt tgatgaagta gtttcattgc
    19081 ttggtttgat tttaggcaag gtaatggtta ataacccatt ttcaaaacta gcagtaatat
    19141 gttgcttatc aacagcttca aaatcaaatt gacgcattaa tgattcgaag ttacgctcat
    19201 ctaaaatgag ttgttcagat ttgtattttg cgcttctagt agcttgaata gtgagcgtat
    19261 tattattgaa atcgatacta atatcttctt tatttacacc tgctagttca gcttctaagt
    19321 aatataattc gtctgtttca tatatattcg ttacaaatga aggtgttgaa aagtaattaa
    19381 atacttgttt acctaaatct ttaaatgtat cacttggatt accgttaaaa aagttttgat
    19441 tctcgaattg attgaagttc aatatcatca cttccattat agaaatttgt attacaaagc
    19501 cattatatga gtagtattaa aaaagttaaa gtaaaagggg agataaaaaa gtaataaatg
    19561 ggtagatttg tgcctgataa ggaatcaggg caaaagaact gagcatcgag atttatacaa
    19621 gcaataaaga taatgaataa acatagcgat gcatacttct gcccatcacc ttatgatagg
    19681 gagttatttt ttgtcggaat agtcaaaatt tttagaaaca aaattccgca aattattaat
    19741 tatatctgat gcttctcgtt ctgaaatgtt gaattcatta aagaccttaa cagaaatttc
    19801 agcaagaggg ctttttattg ctttaccttg ttctgttaga gaaatttgta ggtttctttc
    19861 atctttctct tcacgtgttc gaacaacgta atctttcttt tctaatttct ttagtaatgg
    19921 tgtcagtgtt ccagaatcta agaatacacg ttcacctaat tttttgatgt taagtttttc
    19981 atcattttca atcgccatta aaacaatgta accagtatag gttaaatcat attccttaag
    20041 atagtttgtg tattttttaa ttatttcttt tgaagaaaca taaaataaaa aacacaactg
    20101 tttgctaaga tagctgtttt ctacatacat ccaatcactc cttgttaaaa taaacaatat
    20161 tatgaagtgt aatagttgtc aagtactgat ttcatcgcaa ttattgaatt gtctataatt
    20221 gaatttaaat gttaaataat gcaaaatata tcgatatctt agtattttca tcggtaatta
    20281 ctgatatgac tgatttttta aattgaaaaa aatgtaattg tatcatcctt aattgttaaa
    20341 agtagtcaat tattttgata gatagtaaaa taaaatttgt tattaaattt tttaaaagca
    20401 tcgtgtatgt aattcgattt ctataataat attgagaata aaaaaatttg ttacaatctg
    20461 gtaaaaactc tttattaaat aaattgattc gttcatactg aatagataag caaaaaggag
    20521 ttacataatg aagaagaaaa aaaacgcgat taaagttatc attatattat ttatttcatt
    20581 aattgttgca gtagcatttt tctttggtct gaaaacatat caaggccata agaatattca
    20641 actaattgac agttacttgg aagagaaaaa tctaaaagat aaaattaaat cagaaaaaac
    20701 agagtatagt gctaaaaaag gtctcttcta taaggaagtt acatttaaag atgaacctgg
    20761 tgtaacttat gttgtgcaac caatcagtac taataaagga ttatttgttg aaggctttga
    20821 tacagagact aagaaaagtt tgaaaactgc aaagcataaa tattttaatc aaaattataa
    20881 accatcaaaa tagtgtaacg tataactggt gaaacagttt agtgccttca attttaatta
    20941 tttgaggtaa acagtttttg tatggaaatt agtgattttt gacatctata aaagataaat
    21001 attttaattt tttatagata attatcaatt ttctgatata attgagtgat gtcagatgaa
    21061 ggggagtttg tactatggct ctattcaaga gaaaaattag cctacctatg caagttgtta
    21121 ttgcattagt gttaggtgtt gtcgtaggac ttttattata tggacaagaa aacgtagcaa
    21181 attacattaa accatttggt gacgtatttt taaatttaat taaaatgatc gttataccag
    21241 ttgtattttg ctcactagcg ctttctattt cgaacgttgg ggaatcgaaa actgtagggc
    21301 gttatggctg gaaaacaatt ttatactttg aaattattac aacaatcgca ataggtttag
    21361 ggattatctt cggtaaccta tttaaaccag gtgctggatt agacccaaca aaattaccta
    21421 aaggtgatat ttctaaatat caatcaactg cacatgcagc agaacaatct acatatggaa
    21481 atcattttat tgataccatt gtacatatta ttccgacaaa cttttttgaa gctttaaata
    21541 agggtgaatt attacctatt atcttcttcg cagtattctt tggattagga ttagctgctg
    21601 taggtaaaaa agcagaacca gttaaagaat ttttaagcgg atcgcttgaa gctgtgttct
    21661 ggatgattaa taaaatttta aaattagcac cacttggagt gtttgcattc atttgtacta
    21721 caattattac atttggtgca tccgcattat taccactatt aaaattagta ttagttgttg
    21781 tctttgcaat ggtgttcttt gtattcgcta tactaggact agttgcatgg atgtgtggta
    21841 ttaatatcat gaatattatt agaatcttga aaagtgaatt gcttttagca ttttctacat
    21901 caagttcgga agctgtactt cctgtaatga tgaagaaaat ggaaaacttc ggttctccaa
    21961 aagaaattac ttcttttgtt ataccaattg gttatacgtt taacttagac ggatcagcac
    22021 tttatcaatc tattgcagca ttattcgttg cacagatgta tggaatgcac ttaacattat
    22081 cagagcaaat tgtgttgatg ttaacattaa tgattacgtc taaaggtatg gcggcagtac
    22141 caggaacttc aatcgttgtt ttattaacaa cattaggtgc catgggctta tcggcacaag
    22201 gtttagcatt aattattggt gttgaccgta tcttagatat ggtacgtaca tgtgtaaacg
    22261 ttattggtaa cgcattatca acaatcgtta tagctaaatg ggaaaacgta tatgacaaag
    22321 caaaaggtca agaatattta aaatcaattt aaaaaatact atctgacatt taaagcccct
    22381 aacaaccttt ggttgttagg ggcttttatg catgcgcaaa agcgcaggcg aaccgaagcg
    22441 cagtgacctc aggttgttag gggcttttat gcatgcgcta aagcgcaggc gaaccgaaaa
    22501 tagactgaca cagtaattct taaaaagcag cagtaagatg attccaaatt ggaaatcaac
    22561 ttaccgctgc ttttactcgt atataccttg atggcaacta aaatttgtaa tttaaattac
    22621 aattagtgtt tcatgtgcat tttgcccatg tcatgaattt tcatcattaa accgtgaggg
    22681 acgtcatcat ggtcaacttt tttaacttta acaattttat ctgaatctgc taatttataa
    22741 acaattacaa aatcaccttt tttaacttta acatctgaat ctgaaggtaa tttaacgttc
    22801 ttctcaatgc tttcatcttt ttcactaatc attttttcaa tagtagaatt atctttcata
    22861 attccatagt atgtatcttt ctttccgcca gtcatcatca tgattactaa tacaatacat
    22921 acaattaaca tgatggcgat taaactaata ccaagaccaa aacctttttt ctttttcatg
    22981 gttcatcaat ctccttaaat gtagttaatt ctgttatacc taaaagtgca acagtttgca
    23041 aatgcagtat ttatattaca taaaattaat ttaaaaagac acaccctaat cacgttaaat
    23101 gtgaataaag tgtgtctaaa aaacaataca ttgcaaattg aattgtaaac atattaaaca
    23161 ctttaattat agcgagttat tttaagtatt taacattgtt atatct
//
LOCUS       DAHHYJ010000028        22499 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000028 DAHHYJ010000000
VERSION     DAHHYJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22499
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.028"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(479..1339)
                     /gene="folD"
                     /locus_tag="NBK22_000827"
     CDS             complement(479..1339)
                     /gene="folD"
                     /locus_tag="NBK22_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCD2414030.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPEKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            1540..2022
                     /gene="purE"
                     /locus_tag="NBK22_000828"
     CDS             1540..2022
                     /gene="purE"
                     /locus_tag="NBK22_000828"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCD2414031.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARERGINIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNQYESSLIQKVEDMQNELQ
                     "
     gene            2009..3133
                     /gene="purK"
                     /locus_tag="NBK22_000829"
     CDS             2009..3133
                     /gene="purK"
                     /locus_tag="NBK22_000829"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCD2414032.1"
                     /translation="MSFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVVVLDPSEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITFFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            3137..3841
                     /locus_tag="NBK22_000830"
     CDS             3137..3841
                     /locus_tag="NBK22_000830"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCD2414033.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETEQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHVKLLNIASDEDIEILKSKALKINNVLK
                     QLMDAMNIKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            3841..4104
                     /gene="purS"
                     /locus_tag="NBK22_000831"
     CDS             3841..4104
                     /gene="purS"
                     /locus_tag="NBK22_000831"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCD2414034.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            4106..4777
                     /gene="purQ"
                     /locus_tag="NBK22_000832"
     CDS             4106..4777
                     /gene="purQ"
                     /locus_tag="NBK22_000832"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCD2414035.1"
                     /translation="MKFAVLVFPGSNCDRDVFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            4770..6959
                     /gene="purL"
                     /locus_tag="NBK22_000833"
     CDS             4770..6959
                     /gene="purL"
                     /locus_tag="NBK22_000833"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCD2414036.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            6938..8422
                     /locus_tag="NBK22_000834"
     CDS             6938..8422
                     /locus_tag="NBK22_000834"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCD2414037.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            8415..9443
                     /locus_tag="NBK22_000835"
     CDS             8415..9443
                     /locus_tag="NBK22_000835"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCD2414038.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDSSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            9446..10012
                     /gene="purN"
                     /locus_tag="NBK22_000836"
     CDS             9446..10012
                     /gene="purN"
                     /locus_tag="NBK22_000836"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCD2414039.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHDIPVYINEPKQFDSKAAYEQHLVSLLNEDKVEWIILAGYMRLIGPDLLASF
                     EGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDCGMDTGEIIEQRQCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            10027..11505
                     /gene="purH"
                     /locus_tag="NBK22_000837"
     CDS             10027..11505
                     /gene="purH"
                     /locus_tag="NBK22_000837"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCD2414040.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVSTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKEEMKVVTEVAPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            11527..12774
                     /locus_tag="NBK22_000838"
     CDS             11527..12774
                     /locus_tag="NBK22_000838"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCD2414041.1"
                     /translation="MNVLVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESDHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMKKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKDG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGDTFVTSGGRVILAIGKGDNVQDAQRDAYKK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13039..13845)
                     /locus_tag="NBK22_000839"
     CDS             complement(13039..13845)
                     /locus_tag="NBK22_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD2414042.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITAIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDVIFIIVLIAIITLAYYCSITLPITGIDDVRLGRVG"
     gene            complement(13838..15238)
                     /locus_tag="NBK22_000840"
     CDS             complement(13838..15238)
                     /locus_tag="NBK22_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414043.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIEIWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     EYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLQNVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGALLDCDGDTNV"
     gene            complement(15253..15828)
                     /locus_tag="NBK22_000841"
     CDS             complement(15253..15828)
                     /locus_tag="NBK22_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD2414044.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQSTGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(15905..16005)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16475..16609)
                     /gene="graF"
                     /locus_tag="NBK22_000842"
     CDS             complement(16475..16609)
                     /gene="graF"
                     /locus_tag="NBK22_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCD2414045.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEEKTEDINQTKQDIQDTLN
                     "
     gene            16761..18056
                     /locus_tag="NBK22_000843"
     CDS             16761..18056
                     /locus_tag="NBK22_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414046.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     SGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            18481..19653
                     /locus_tag="NBK22_000844"
     CDS             18481..19653
                     /locus_tag="NBK22_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCD2414047.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAEREYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLINGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            19707..20249
                     /locus_tag="NBK22_000845"
     CDS             19707..20249
                     /locus_tag="NBK22_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCD2414048.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRQR
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            20403..20669
                     /locus_tag="NBK22_000846"
     CDS             20403..20669
                     /locus_tag="NBK22_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCD2414049.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            20672..22390
                     /gene="ptsP"
                     /locus_tag="NBK22_000847"
     CDS             20672..22390
                     /gene="ptsP"
                     /locus_tag="NBK22_000847"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCD2414050.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNSAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ATALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWIGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
BASE COUNT         7890 a         3269 c         4248 g         7092 t
ORIGIN      
        1 tcaaaacaat aagaaaaggg aggcgaaaat gataaagaac cactaatgat taactatgta
       61 gtagtacttt atcatagtca cagcttctgt gatggggccc ccaccccaac tcgcattgcc
      121 tgtagaattt cttttcgaaa ttctctgtgt tgggacccct ccccaacttg cacattattg
      181 taagctgact tttcgtcagc ttctttgttg gggcccctga ctagaattga aaacttgttg
      241 caagcttatt ttcattcaat taactactgt ctatataaaa ttgttgagcc aagaacaaag
      301 tgaaatgctc ccttcaaagc cttcatttat tcaatgtcta ctttgaaggg agcatttgat
      361 cgattaatgt cccagatttt tcttgttatt catttcaatt gcgaaataat attaaatgtg
      421 ggttaaaaga cttcattaaa actcagttat taattctcaa tacataacta atgtaaattt
      481 acgaatcaat acctcgacgc attttttctg caagcaaagt attatttaat accatagtaa
      541 ttgttaatgg accaacgcca ccaggaactg gtgtaatagc tccagcaatt tctttaaccg
      601 catcataatc aacgtcacct tttaatttgc cattttcatc tggcgtattg ccaacatcga
      661 taattactgc tccttctttg accacatctt ttgttactaa accaggctta ccaactgcac
      721 tgacaatgac atcagcatct tttaaatatg atgccatatc ttttgaacga gaatgtaaga
      781 ttgttactga tgcatttttt tgaagtagta acttagaaac tggttgtccg acaatatgac
      841 ttcgtccaat tacaactgca tttttacctt ctaaatcaat atctgcatgt tttaatattt
      901 ccatgatgcc gagcggtgtg caaggtacaa aagtttgttc atcgatatat aatttcccta
      961 tatttattgg atgaaatccg tccacatctt tttcaggatt gattgcttct aatattttct
     1021 gttcgctaac ttgttttggt aatggtactt gtaccaaaat accacttaca gaatcatcat
     1081 tatttagtct atttagttcg tttaatactt cttcttcagt agctgtttct tccaaatgta
     1141 cgatttctga aatcatacca attttttcag ctgctttctt ttttgatcta acataacttt
     1201 gactagcgcc atcattacca actaatataa cggataattt aggtgtaaaa cccttttctt
     1261 ttagcgcttc aacttgatct tgtaacccct gtctgtagtc tttggcaatt tgtttaccat
     1321 ctaaaatttt agcaaccata aaaacttctc ctcctattgt caatatccta actttgttag
     1381 ttcataaaac taatttacat agcaaaagac gtaaattcaa acaaaaaatc aaaataaagt
     1441 tcgatttttg attgaaaaag cagaaattgc ttgttatgct atatctataa tatacaacta
     1501 aagtaattca caattcaaac ttttgaaagg gtgtacaaag tgaaagtagc agtcattatg
     1561 ggcagttctt ccgattggaa aattatgcaa gagagttgta acatgttgga ttattttgaa
     1621 attccgtacg aaaaacaagt agtatccgca catcgtacgc caaaaatgat ggttcaattt
     1681 gcttctgaag cgagagaaag aggtataaac attatcattg caggcgctgg cggtgcggca
     1741 catttaccag gtatggttgc atcattaacg acgctaccag ttattggtgt gccgattgaa
     1801 acaaaaagtt taaagggtat agattcttta ttatcaattg ttcaaatgcc aggaggtatt
     1861 ccggttgcaa cgactgcaat tggtgcagca ggtgctaaaa acgcaggtat acttgcagca
     1921 agaatgttaa gtattcaaaa tccttcttta gttgaaaaac taaatcagta tgaatcttcg
     1981 ttaattcaaa aagtggagga catgcaaaat gagcttcaat aaattaaagt ttggtgcgac
     2041 tattggcatt attggtggtg gtcagcttgg aaagatgatg gcacaatcag ctcaaaaaat
     2101 gggttataaa gtggttgtat tggatccttc tgaagattgt ccatgtagat acgttgcaca
     2161 cgaatttata caagccaagt atgacgatga aaaggcactc aatcaattag gacaaaaatg
     2221 tgatgtgatt acttatgaat ttgaaaacat ttcagcccaa caattaaaac tattatgtga
     2281 aaagtacaat attccgcaag gttaccaagc tatacagtta ttacaagacc gcttaactga
     2341 aaaagaaaca ttaaaaagtg ctggtaccaa agttgtcccg ttcatttcag taaaagaatc
     2401 tacagatatt gacaaagcaa ttgaaacatt aggatatcct tttattgtaa aaactagatt
     2461 tggtggctac gatggcaaag gtcaagtttt aattaacaac gaaaaagact tacaagaagg
     2521 ttttaaatta attgaaacta gtgaatgcgt agctgaaaaa tatttgaata tcaagaaaga
     2581 agtatctctt actgttacaa gaggaaacaa caatcaaatc acttttttcc cattacaaga
     2641 aaatgagcat agaaatcaaa tacttttcaa aacaattgtt ccagcgagaa tagataaaac
     2701 agctgaggcg aaagagcaag ttaataaaat tatccaatcg attcatttca ttggaacatt
     2761 tacagttgaa ttttttatag atagtaacaa ccaattgtat gtgaacgaga tagcaccaag
     2821 gcctcacaat tccggacatt attcaattga agcatgtgat tattcacaat ttgatactca
     2881 tattttagca gttaccggac aatcattacc aaattcaatt gaattattaa agccagcagt
     2941 catgatgaac ttactaggta aagatttaga tttattggaa aatgaattta atgaacatcc
     3001 agagtggcac ttacatattt atggtaagtc tgagcgtaaa gatagcagaa aaatggggca
     3061 tatgactgta ctaacgaatg atgtaaacca aactgaacaa gatatgtacg ctaaatttga
     3121 ggggagtaat taaagcatga cattattata tgaaggaaaa gcgaagcgca ttttctcaac
     3181 aaatcaagaa aatgaattaa gagttgaata taaagatgaa gttactgctg gaaacggggc
     3241 taagaaagac acaatggcag gtaaggggcg attaaataat caaattactt ctattatatt
     3301 taaatattta caagaaaatg gaatagaaag tcactttatt aaacaattat ctgaaacaga
     3361 acaattagtt aagcctgtga aaataattcc attagaagta gttgttcgta atattgctag
     3421 tggatctatt acaaagcgtt taggttttga aaatggtgaa gtttttagag aaccacttgt
     3481 agaatttttc tataaaaatg atgcgttaaa tgatccgttg ataacggatg accatgttaa
     3541 attgctcaat atagcatcag atgaagatat tgaaatacta aaatccaaag cattaaagat
     3601 taataatgtg ttgaaacaat taatggatgc tatgaatata aaattagtag attttaaaat
     3661 cgaatttgga aagactgaga ctggtcaaat tttgttagcg gatgaaatat ctccagatac
     3721 atgtcgaatt tgggataaag ctaccaatgc aaactttgat aaagatgtat atagaaataa
     3781 cactggatca ctgattgaaa catatcaaat atttttaaac aaattggagg atttaaaata
     3841 atgaaaacaa ttgaactaca tatcacatta caaccacaag tattagatac gcaaggacaa
     3901 acgcttactc gagctgtaca tgacttaggt tatgcacaag tgaatgatat tcgtgtagga
     3961 aaagtattat atatgacagt ggatgaggtt agtgatgaaa aggtacacaa cattattaca
     4021 actctaagtg aaaaattgtt tgcaaataca gtgattgaag aatatagcta taaagtgtta
     4081 gatgatgaaa aggagaatgc ataaaatgaa atttgcggtt cttgtttttc caggttcgaa
     4141 ttgtgataga gacgtgttta atgctgctat taaaagtggt gttgaagcgg aatatgtaga
     4201 ttatagagaa acatcactaa gtggatttga tggcgtactt attcctggtg gattttcatt
     4261 cggggattac ttaagatctg gggcaatggc tagtgtagcg ccgattattt cggaagttaa
     4321 acgtcttgca gctgaaggta agccagtatt aggtgtttgt aatgggtttc aaattttaac
     4381 tgaaataggc ttattacctg gtgcattatt gcataacgat tcacatttat ttattagtag
     4441 aaatgaagag ttagaaatag tgaataatca aacggcattt acaaatcttt atgaacaagg
     4501 tgaaaaagtt atatatcctg tagctcacgg tgaaggtcat tattattgta ctgatgaaat
     4561 atatcaacaa ttaaaagcta acaatcaaat tattctgaaa tatgtgaata atccgaacgg
     4621 ttcatatgat gatattgcag gaattgttaa cgaaaaaggc aatgtatgtg gcatgatgcc
     4681 acatcctgaa agagctttag aaacgttgtt aggtactgat agtggtgtga aattatttga
     4741 agcgatggta aaaagttgga gggaacaaca tgtctaaatt tatcgaacca agcgttgaag
     4801 aaattaaact tgaaaaagta tatcaagata tgggattaag tgatcaagaa tatgaaaaag
     4861 tttgcgatat tttaggcaga caacctaact ttacagaaac aggtatcttt tctgttatgt
     4921 ggagtgaaca ttgctcttat aaacattcta aaccgttttt aaagcaattt cctacgtcag
     4981 gtgaccatgt gcttatgggg cctggtgaag gtgcaggggt agtcgatata ggtgataatc
     5041 aagccgtagt atttaaagta gagtctcaca atcatccatc agcaattgaa ccatatcaag
     5101 gggctgctac aggcgttggt ggaatcattc gtgacattgt ctctattggg gctagaccta
     5161 ttaatttgtt aaacagtctt agatttggag aattagataa taaacaaaac caaagattac
     5221 ttaaaggtgt tgtaaagggt atcggaggtt atggtaactg cattggtatt ccaacaactg
     5281 ctggtgaaat cgaatttgat gaacgttatg atggcaatcc acttgttaat gcaatgtgtg
     5341 ttggtgttat caatcacgac atgattcaaa aaggcacagc aaaaggtgta ggtaattcgg
     5401 tcatttatgt tggtttgaaa actggtcgag atggtattca tggtgctact tttgcatctg
     5461 aagaattgac ggaagaaagc gaaagtaaac gaccttctgt acaaatcggt gatccatttg
     5521 taggtaaaaa attaatggaa gcaacacttg aagcaattac atttgatgaa ttagttggta
     5581 ttcaagatat gggtgctgct ggtttaacat cttcatcgtc tgaaatggcg gcaaaaggtg
     5641 gtagtgggtt acatttgaga ttagaacaag tgccaacacg tgagccaggt atttctcctt
     5701 atgaaatgat gctttcagaa actcaagaac gtatgttact agttgttgaa aaaggtactg
     5761 aacaaaaatt cttagattta tttgataagc acgaattgga tagtgctgtt ataggtgaag
     5821 ttacagatac aaatcgtttt gttttaacat atgatgacga agtttatgct gacattccag
     5881 ttgaaccact agctgatgaa gcacctgtat atattttaga aggagaagaa aaagattata
     5941 atacttctaa aaatgattat acacacatcg atgttaaaga tactttcttt aaattactta
     6001 agcatccgac tatagcatct aaacactatt tatatgatca atacgaccaa caagttggtg
     6061 ccaatacgat aattaagcca ggacttcaag catcggtagt acgtgtggaa ggcacaaata
     6121 aggcaattgc ttcaacaatt gatggtgaag cgcgttatgt atataacaat ccatatgaag
     6181 gtggaaagat ggtagtagct gaagcttatc gaaatttaat tgccgtgggt gcaacaccat
     6241 tagcaatgac agattgttta aattatggtt ctcctgaaaa gaaagaaatc tatcaacagt
     6301 tgatagattc aacgaaaggt atggcagaag catgcgacat tcttaagaca ccagtagttt
     6361 ctggtaatgt atctttatat aacgaaacga aaggtacttc tattttccca acaccagttg
     6421 ttggaatggt aggtttgatt gaaaatgtaa attatttaaa tgattttgaa cctcaagttg
     6481 gagataaatt atatttaatc ggtgatacta aggacgactt tggtggtagt caacttgaaa
     6541 agttaattta tggcaaagtt aatcatgaat ttgagtcatt agatttgagt tcagaagttg
     6601 aaaaaggtga atcaatcaag accgctattc gtgaaggact attatcacat gttcaaacag
     6661 ttggtaaagg tggcttactg attaccttag ctaaactaag tgcgcattac ggtttaggat
     6721 taaaatcttc aatagatata acaaatgcac aattgtttag tgagacgcaa ggccgatatg
     6781 ttgtttctgt taaatcaggt aaaactttaa atattgataa tgcaatagaa attggacttt
     6841 taacagatag tgataatttc aaggtaacaa caccatatac agagattagt gaaaatgttt
     6901 cagatattaa acaaatatgg gaaggggcaa ttgctcaatg tttaactact caggattaaa
     6961 cgaagaatgt ggcgtgtttg gtatttggaa tcatcctgaa gcagcgcaac taacatatat
     7021 gggacttcat agtttgcaac atcgtggtca agaaggtgca ggtatagttg tttctgatca
     7081 aaatgaatta aaaggcgagc gaggattagg cttactaact gaagcgatta aagatgatca
     7141 aatggaacga ttaaaaggat atcaacatgc aattggtcac gtccgttatg ctacttcagg
     7201 taataaaggt attgaaaata ttcaaccgtt tctgtatcac ttttatgata tgagtgtagg
     7261 tatttgtcat aatggtaacc tcattaatgc taaatcattg cgtcagaatt tagaaaaaca
     7321 aggtgctatc ttccattcgt cttctgatac tgaagtcatt atgcatttga tacgtcgaag
     7381 taaagctcct acttttgagg aagcgttgaa agaaagtttg cgaaaagtta aaggcggttt
     7441 tacatttgcg attttaacta aagatgcgtt atatggcgca gtagatccaa atgctatcag
     7501 accacttgtt gtaggtaaaa tgaaagatgg gacatacatc cttgcaagtg aaacatgtgc
     7561 aatagatgtg ttaggtgcag aatttgttca agatattcat gcaggtgaat atgtcgtgat
     7621 taacgataaa ggtattacag ttaaatctta tacacatcat acgacaactg caatttctgc
     7681 gatggaatat atttattttg ctagaccaga ctcaacaata gctggtaaaa atgtccatgc
     7741 agtacgtaaa gcttctggta aaaaattagc ccaagaaagc cctgtaaatg ctgatatggt
     7801 catcggtgta cccaattcat cgctatcagc tgcgagtggt tatgctgaag aaataggttt
     7861 gccatatgaa atgggactag ttaaaaatca atatgttgca agaacattta ttcaaccaac
     7921 tcaagaatta cgtgagcaag gtgtgagagt gaagttatct gcggtaaaag atatagtaga
     7981 tgggaaaaat atcattcttg ttgatgattc cattgttcgc ggtacgacaa ttcgacgcat
     8041 tgtgaaaatg ttaaaagatt ctggtgcaaa taaagtgcat gtgcgtatag catcaccgga
     8101 atttatgttc ccaagttttt atggaatcga tgtttcaact acggcagaat taatttctgc
     8161 aagcaaatca cctgaagaaa ttaaagatta tattggcgct gattcattag catatctatc
     8221 tgtagatggg ttaattgaat caattggttt agattatgac gcgccatata gtggcttatg
     8281 tgtagaaagt ttcactggag attatcctgc agggttatat gattatgaag caaattataa
     8341 agcacattta agtcatcgac aaaagcaata tatttctaaa aacaaacact tttttgatag
     8401 cgagggaaat ttaaatgtct aaagcatatg aacaatctgg tgtaaatatt catgctggtt
     8461 atgaagctgt agaaagaatg tctagtcatg ttaaacgtac gatgcgtaaa gaagttatcg
     8521 gtggtttagg tggattcggt gctacatttg atttatcaca attaaatatg acagcgccag
     8581 ttttagtttc tggaacagac ggagtaggta cgaaattaaa actagctatc gactatggga
     8641 aacatgattc gataggtatc gatgcagtcg caatgtgtgt taatgatatt ttaacgacag
     8701 gtgcagaacc attgtatttt ttagattaca tcgctacaaa taaagttgtt cctgaagtta
     8761 ttgaacaaat tgttaaaggt attagtgatg catgcgttga aacgaatact gcacttatcg
     8821 gtggagagac tgctgaaatg ggtgaaatgt atcacgaagg tgaatatgat gtagccggat
     8881 ttgcagttgg agcagttgaa aaggatgact atgtagatag ttcagaagtg aaagagggac
     8941 aagttgttat agggcttgcg tcaagtggca ttcattcaaa tggatatagt ttagtgcgca
     9001 aattaattaa tgaatcaggc attgatttgg catcaaactt cgataatcgt ccatttatag
     9061 atgtcttttt agaaccaact aaattatatg tcaaacctgt acttgctttg aaaaaagaag
     9121 tttctattaa ggcaatgaat catattactg gtggaggttt ttatgaaaat attccacgtg
     9181 cattgccagc cggatatgct gctagaattg atactacatc atttccaacg ccaaaaatat
     9241 ttgattggtt acaacaacaa ggcaatatag acacaaatga aatgtataac atttttaaca
     9301 tgggtattgg ctatacggtt atcgttgatg aaaaagatgc atcacgcgct ttgaagattt
     9361 tagcagaaca aaatgtggaa gcctatcaaa ttggtcatat tgtgaaaaat gagtcaactg
     9421 caattgaatt gttgggggta taacaatggt taaaattgcg atttttgcat caggttcagg
     9481 aagtaacttt gaaaatatag ttgagcatgt tgaatcagga aaacttgaaa atattgaagt
     9541 tacggcgcta tatacggatc atcaaaatgc gttttgtata gatagagcaa aaaagcacga
     9601 tattcctgtt tatattaatg aaccaaaaca atttgattca aaagcagcgt atgaacaaca
     9661 tttagtatca ctattaaatg aagataaggt agagtggatt attttagctg gctacatgcg
     9721 tctaattggt ccagatttat tagcttcatt tgaaggtaaa atattgaata tacatccatc
     9781 tctattgccg aaatataagg ggattgacgc aataggccaa gcatatcata gtggcgatac
     9841 tattactggt tcgacagtac attatgttga ttgtggtatg gatacgggag aaattattga
     9901 acagagacaa tgtgatatta gaccggacga ttcaaaagaa caattagaag agaaagtaaa
     9961 aaaattggaa tatgagttat atccaagtgt tattgctaaa attgtaaaat aaggagtatg
    10021 tatgtgatga agaaagctat tttgagcgta tcaaataaaa caggtattgt agagtttgct
    10081 aaagcgttaa cgcaattaaa ttatgaatta tattcaactg gtggtactaa acgtatatta
    10141 gatgaagcaa atgtaccagt tcgttctgtt tcagacttaa cacattttcc agaaataatg
    10201 gatggccgtg ttaaaacatt acatccggct gttcatggtg gcattttagc tgatcgaaat
    10261 aaaccgcagc atttaaatga attatcagaa caacatatag atttaattga tatggtagta
    10321 gttaatttat atccattcca acaaactgtt gcaaaccctg atgtgacgat ggacgaagca
    10381 attgaaaata ttgatattgg tggtccaaca atgttacgtg ctgcagctaa aaactataaa
    10441 catgtaacaa caattgtaca tccggcagat tatcatgaag tatcgacgcg attaagaaac
    10501 gattcgttag atgagtcata tagacaatca ttaatgatta aagtttttga gcatactgca
    10561 gaatatgatg aagcgattgt acgtttcttt aaaggggata aagaaacttt aagatatgga
    10621 gaaaatccac aacaatcagc gtattttgtg agaacttcga atgctaagca cacgattgca
    10681 ggcgctaaac aattacatgg gaaacaatta agctataaca atattaaaga tgcagatgct
    10741 acactagctt tagttaaaaa gtttgataca cctgctgcag ttgcggttaa acacatgaat
    10801 ccatgtggtg ttggtatcgg tgacacgata gaacaagcat ttcaacatgc gtatgaagcg
    10861 gatagtcaat caatatttgg tggaattgtt gcattaaacc gagctgtaac acctgagtta
    10921 gcagagcaat tgcatagtat ctttttggaa gtcattattg caccaaaatt tacagatgaa
    10981 gcattagata ttttaaaaca aaagaagaat gtaagattat tagaaattga tatgactata
    11041 gacagtaacg aagaagagtt tgtttcagta tctggcggat atttagttca agataaagac
    11101 aattatgtcg tgccaaaaga agaaatgaaa gttgttacag aagttgcacc tactgatgaa
    11161 caatgggaag caatgttatt aggatggaaa gttgtaccat cagtaaaaag taatgcaatt
    11221 attttaagta ataataaaca aactgtaggt ataggtgctg gacaaatgaa tcgtgtcggt
    11281 gctgctaaaa ttgcgttaga gagagctatt gaaatcaatg atcatgtagc gttagtatct
    11341 gatggatttt tccctatggg agatacagtt gaacttgcag cacaacatgg tataaaggca
    11401 attatccaac cgggtggttc gattaaagat caagattcaa ttgatatggc taataaacat
    11461 ggtattgcaa tggtagtcac aggcactcga cattttaaac actaatttta aaggggcgta
    11521 acataaatga atgtattagt aattggtgct ggtggacgag aacatgcact tgcatataaa
    11581 cttaatcaat cgaatctagt taaacaagtg tttgtcattc caggtaatga ggcaatgaca
    11641 cctatagctg aagtacacac tgaaatttca gaatctgatc atcaaggaat actagatttt
    11701 gctaaacagc aaaatgttga ttgggtagtt ataggtccag aacagccgct aattgatgga
    11761 ttagcagaca ttttacgagc gaatggtttc aaagtgtttg gtccaaataa gcaagcagct
    11821 caaatcgaag gctcaaaatt atttgctaaa aagataatga aaaaatataa tattccaact
    11881 gctgattata aagaagttga gcgaaaaaag gatgctttaa catatattga aaactgtgaa
    11941 ttgcccgttg ttgtcaagaa agatgggtta gctgctggga aaggcgttat tattgcagat
    12001 actattgaag cagccagaag tgctattgag attatgtatg gtgatgaaga agaaggtact
    12061 gttgtatttg aaacgttttt agaaggtgaa gagttctcgc taatgacatt tgttaatggt
    12121 gatttagcag tacctttcga ctgtattgca caagatcata aacgcgcatt tgatcatgat
    12181 gaaggaccaa atactggtgg tatgggggct tattgtccag taccacatat tagtgacgat
    12241 gttttaaaac ttacaaatga aacaattgca caacccattg caaaggcaat gcttaatgaa
    12301 ggttatcaat tcttcggtgt attatacatt ggtgctattt taactaaaga tggtccaaaa
    12361 gtaatagaat ttaatgcccg ttttggtgat cctgaagctc aagtattatt aagtcgcatg
    12421 gaaagtgatt taatgcagca tattattgat ttagatgaag gaaaacgtac tgaattcaaa
    12481 tggaaaaatg aatctattgt aggggtcatg ttggcatcaa aaggatatcc tgatgcatat
    12541 gaaaaagggc ataaagtaag tggctttgat ttaaatgaaa actattttgt tagtggatta
    12601 aagaagcaag gtgatacctt tgttacttca ggtggtagag ttatacttgc catcggaaaa
    12661 ggtgacaatg tacaagatgc acagcgagac gcatacaaaa aagtatcaca aatacaaagt
    12721 gaccatttat tctatcgtca tgacattgcg aataaagcac tacaacttaa ataagtaaat
    12781 ttaaaatact aagattagct atgaacgaat ctataacgat agattttttc atagcttttt
    12841 tagttgtaga gtctaggaca ttgatttctg taccaaattt gtgattatgc atatgtaata
    12901 caaaagaggc gccacaacat gtttggatga acaaaataac atgtttgtgg cacctctttt
    12961 gtttagtatg gaataaatgg ttttcttttt ctatacaatg aatttctaat ttagtatcta
    13021 tacaattatg gataaaattt aacctacacg accaagacga acatcatcta tgcccgtgat
    13081 gggtaaggtg attgaacaat aatatgccaa agtaataatg gcaattaaaa ctataataaa
    13141 gattacatct ttatatgaga aaggtacgtt gtaatagtaa gtacgaggac catctctaaa
    13201 tcctttcgac tccatcgcaa ctgataattg atgtgccttt ctaatatttt ggcttaatag
    13261 aggtataatt aaatgcttaa atcgctttaa ccctctataa tttgccgcgt ctatcatctg
    13321 atagcgcatt tttaaagatc tgcgaagctg tattaaagaa ctaatcatta aaggtatcat
    13381 acgaatggca gccatgaatg cataagcaac ttttgattta acctttaaat gttgcattaa
    13441 actataaaat atcatgacaa cctgagatgt aagtgcgatt aagataccga aaaatgaaat
    13501 agcaatggtt cttaatgata catgtaaacc acgaactaaa ctttctgttg taatatggat
    13561 aaatccgaat ttcaaaattg tatggctacc attcccgtat aaaatcatga acagggaaga
    13621 gagtaatgca aagccaatac ttatagttat aaaaattgct gtaattttaa actgagtacc
    13681 attaaacatc aataagaaaa ctaacattaa gatagtgata taaagcataa aatcgaaatt
    13741 atgcacaaat ataataaaga aaaatagtat aattccaaga aatagtttcg ttataatgtt
    13801 gacatcatca acaaatgatt gccgaacttt ccattgctca tacattcgta tcaccatcac
    13861 aatctagtaa cgcaccatct gaaattttaa gtcttcttga tggataacgt tcaattattt
    13921 catcgtcatg tgtaaccatg acaatacttt gtcccaaatt aattcgcttt tggaaaagtt
    13981 tgatcaactg gaatgtatta tggctatcaa gtccaaatgt cggttcatct aaaaagataa
    14041 tatcagcttt agaacttagt gcggtagcta cgctaaggcg tcgtttttga ccaatagaca
    14101 actcataagg atgttgatct tttacatttt gtaaatctaa aagttttaaa agttgtatcg
    14161 tttcatcatc actttgatct ttagaaaggt gattaaaatg aatgttaatt tcatcataaa
    14221 ccgaatttgt tataaattgt aattctgggt tttgataaac taggtacatg tgttttgctg
    14281 catgtttaat ttttgttaaa cgctgatttt caaaataaac atcaccttga tatttaatca
    14341 attgcataat tgattcaagc aaggttgttt taccactacc atttgcccct gtaattgtaa
    14401 tccactcacc tagaccaatt tctaaatctg agaatgagag caaggttgat ttaccgcgaa
    14461 taatacgtcc atttttaaat tgtaataagt gtgagtttgt tgttggaaag tcaacacgac
    14521 ttggtgcgaa ttcccatgca cgtggatgcc acacaccata ttcactgagt aaatgaacat
    14581 actcctgtaa tatgatttca ggacattcat cggcaatgat atttccgtta taatccatca
    14641 aaatgacgcg gtcgacatga ttccagatgt gtttaacttt atgttcaacg attacaacag
    14701 tttgatcttc ccaaatttca attagtttag tccataaatc ttctgttgct tgaacatcta
    14761 acattgctgt cggttcatct aaaaacaatg tttttgattg ttgaagaatg gtttcaacaa
    14821 ttgccaattt ctgtttcatc ccgccactta aatctttgat atacgtttca ggggtaacat
    14881 ttaaattgac catatttaaa gcattgataa ttaacgcatc catgtcttca cgtggtactt
    14941 gtctattttc taaaacgaat gcaagttctt cgtatacttt tggcatacaa aactggctat
    15001 cagggtcttg gaaaataacg ccacttaatg ggtcaacgat tagttcatca tatttcatag
    15061 gtaattcaat taaattagga acaataccac ttaatacatt cagaagtgta cttttaccgc
    15121 aaccagaagg accgagtaaa agtacttttt ctttgtcttg aatagtgata tttaaatgat
    15181 cgaaaatttt acgctgacca cttggatatt ttaatcgtaa atcacttact tttaacactc
    15241 tgaatgctcc tcttataagt tgtcgtaatc ttctttagca gctggtctaa ataattttgt
    15301 tacgcctgtc ttatctaaag cttttactaa aaggtaagat aggacgccgg cgactactgc
    15361 accactaatt aatctaaata cgatgaataa tgttaagttc caacctgcaa cttcatttaa
    15421 ataaccatag aaataatcta tagggaaagc cgcgattgct gtacaaaaac ctgctaacat
    15481 agctaccata actgaacgag attgatattt aaaaattgca aagacaagtt cacacgctaa
    15541 accttgtatg aaagcgtaaa cgattgtcgg aatatcgaaa cgacccataa tgatagtttc
    15601 gccggcacct gcagcaaatt cagccagtaa agcaatacct ggttttggaa taattagata
    15661 gcagacaatc gctgccatga accaaacccc gtttgttaat tgttcgaggt gaaggcctgt
    15721 agattgcaca ccattgtaaa caaaccacca taaattgtaa ataactgcga atactactga
    15781 aataagtacg gttactagta tttcagatag ctttaaacct tttgacattt ttacatcctc
    15841 ctaataaaaa aacgcacaac catccatagg aaagttatgc gttcacaata tatattagta
    15901 aaacatatgt atagtaacac tttcctacgc tagttcaagc tagatcaggt tcaaagggtt
    15961 tgagggcaag cctcatctca gtataaaaca cccctagtgt gtgcgattta tttaattaat
    16021 tatactgtaa gacgtttgta aacttatgtc aataggttgt cttcatgaaa tttcgtttaa
    16081 ttcgatttaa aatttataat attagcattg gatttaaatt gaagatgtag taggaatgtt
    16141 agtaattaaa gatataaaaa tatgtgacat gtaataagat tgagctgata aatgaagagg
    16201 gatacttatc aatcatacct ctttaacaac agtgaagaac ccgtgcataa tggcttacga
    16261 attatagttt gtagggtaaa agagggatac atgcgccgag cacatgaaaa aaagccccta
    16321 acaactaaaa gttgtaaggt aaaagaggga tacatgcgcc gagcgcaagc aaaaaggccc
    16381 ctaacaacta aaagttgtaa ggtaaaagag ggatacatgc gccgagcaca tgcataaaat
    16441 cccctaacaa ctaaaagttg taaggggatt taaattaatt tagtgtatct tggatatctt
    16501 gttttgtttg attaatatct tctgtttttt cttctttttt atcttttaat ttttcttcaa
    16561 cttctttagc tttttctgct gcttttttat tttgattttc attagacatg attaattcct
    16621 cccaaattgg ataattattt atatataaat cttacccggt tgtactttcg ttaaactttt
    16681 ctaagtctat agcactattt attcatttat ctaaagacaa caacattaga ttaatatata
    16741 atgattttga ggtgaacata atgtcttttc ttaggaaaca cgccgaaatt atttttagct
    16801 atttaatcgg tatcgtttca ctcttcactg gtctcattat tttaattaac ttgcctttaa
    16861 ttaaacaatt aaatggtggt aaaaaagttg atacacatgt tcataatgtg tgggaatttc
    16921 tgaatgcatt tttcagtgaa attattaaag taatgagtcg atttataggt aatttcccta
    16981 tagttagtgc aattgtgata attatattcg gtattttagt tatgttgatt ggtcatacat
    17041 tacttagaac tattaagtat gactatgata tttctatctt tttcttagtt atcggtatca
    17101 tgtactttat tattactctt atattaatga ctcaagttta tggattcttt gcagtgattt
    17161 tcattattcc atttacaatt catataggat atatcgtcta taaagatgaa ttgaatcagg
    17221 aaaatgtaaa aaatcatttc atgtggataa ttgtgagtta tggtataagt tacttaatta
    17281 cacaaattgc attgtatggc agaattgatg ctaatgaaat agagtcaatt gatatcttaa
    17341 gtgtcaatgc tttctttata attatgtggt tacttggtca aatggctatt tggaatttct
    17401 tgttcttgcg ccgagcttta cctttaacaa agcaagaatt aggtgaagag gagccagaat
    17461 tatcaagaac aagtaaaggg aatgtcacga atcaaactaa aattcacttg aaacaactcc
    17521 aagataagac tacagaatat gcacgtaaga caagaagaag tgtcgattta gataaaatta
    17581 gagctaaaag agataaattc aaaaagaaag ttaatgatat tatcgatatt caagaagacg
    17641 atattcctga ttggatgaga aaaccgaaat gggttaaacc aatgtatgtc gaactatttt
    17701 gtggtgtcgt catcttttta ttcacatttt tagaatttaa taatcgtaat gcattatttg
    17761 tatctggtga ttggaaatta tcacagacac aatatgttat tgaatgggtt acattattaa
    17821 ttctgttatt cattattatc gcatatatcg ctacaacgtt aactttccac ttgaaaggta
    17881 agttttatta tttacaacta tttatgggga gcattttatt ctttaaattg ttaacggaat
    17941 ttataaatat aatgattcac ggactactac tttcagtgtt cattacgcca acattactat
    18001 taatgttatt ggcaatcatc atttcttatt cgttacaatt acgagagcga ccataattaa
    18061 aagcattata aaagtactat ctattaaaca ttttgatgtg tacgctataa gttagatata
    18121 tctctaactt acttagatac aggtcaatga agtttatgga tagtactttt tttgtaacta
    18181 gatttgattg attcaggtga tgcgaattaa gtattgaaaa ttgtatacaa agttttagtg
    18241 caaataaaat agttgaaaag ttatccattt gtaaaatcaa gaaaactagt aaatagttga
    18301 agcgacatat ggaatttgcg aaacgatata tagtatttcc tttgtagaaa tttaacatat
    18361 atcattcgaa ttactaattt gttaaaatca acagtaagat tagaagtaga tgatattgaa
    18421 atttggcaaa caatttaatc tatataaaac tacaacgaaa cacagaaagg aagttgtcag
    18481 atgaaaatag caactctgaa caaaggcaaa gaaacaaaat attttaatgg atatccttta
    18541 attgaagaag aggatatcta ttcacaagat catttaaaag aaggagatat ttttcaaatt
    18601 gtgactgata aatcacaata tgttgcaacg gcttatgttg gtcgccaaca taaaggatta
    18661 ggttgggttc taacatacga taaagctcaa gaaatcaaca cagctttctt tgtgaaattg
    18721 tttaatactg cattagcaga acgtgaatat tattttaata tagatggaac aaatgctttt
    18781 agattattta atgctgaagg tgatggtgtt gggggattaa caatcgacaa ttatgatggt
    18841 catttgttga ttcaatggta ctcaaaaggt atttataaat ttaaatatgc cattcttgaa
    18901 gcggttagaa aagtatttga ttataaatct atttacgaaa aagtaagatt taaagacagc
    18961 gaatatagtg gtggttttgt tgaaggagat gcacctgagt ttccaattgt tatcgaagaa
    19021 aacttcacat tttataatgt agaccttgaa gatggtttga tgacaggtat ctttttagat
    19081 caaaaagaag tgcgcaagaa attaagggat caatatgcca aagaacgcca tgttttaaac
    19141 ttatttagtt atacaggtgc tttttctgta atagcagcaa gtgaggcatc ttcaacaaca
    19201 agtgtagatt tggctaatcg ttctcgtagt ttaactgaag aaaattttgg attaaatgct
    19261 attgatccta aatcccaata tatttatgtc atggacactt ttgatttcta taaatatgct
    19321 gcacgacatg gacatagtta tgacacgatc gtgattgatc cacctagctt tgcgcgtaac
    19381 aaaaaacgta cattttcagt gcaaaaagat tatgacaaat taattaatgg cgccttaaat
    19441 atcttatcat ctgaaggaac attattgtta tgtacaaacg caagtgtata tccattaaag
    19501 caatttaaaa atactattaa aaagacgctt gaagagagtg gcgttgatta tgaattaact
    19561 gaagttatgg gattaccaaa agattttaaa acgcatccac attataagcc atctaaatat
    19621 ttaaaagctg tttttgtaaa tattagacat taatttgata aaaaggggta tagtaactta
    19681 ttgagaaaaa gaagggtgat aatattatgg gattcaaaaa caatttaaca tcaaatttaa
    19741 caaataaaat tggtaattca gtctttaaaa tagaaaatgt tgacggaaaa ggtgcaatgc
    19801 caacgacgat tcaagaattg agagaaagac gacaacgtgc tgaagcaatt gtaaagagaa
    19861 agtctttaat gtcatcaaca atgagcgttg ttccaattcc gggtttagat tttggtgttg
    19921 atttaaaatt aatgaaagat attatcgaag atgtaaataa aatatacgga ttagatcata
    19981 agcaagttaa tagccttggg gatgatgtga aagaaagaat tatgtctgca gcagcaattc
    20041 aaggtagtca atttattggt aaaagaattt caagtgcatt tttaaaaata gttattagag
    20101 atgtagctaa acgtactgct gcaaaacaaa caaaatggtt tcctgttgta ggacaagctg
    20161 tgtctgcatc tattagttac tattttatga ataaaattgg aaaagatcac attcaaaaat
    20221 gcgaaaatgt tattaaaaat gtcatgtagg tgctataata gttttgcaat ttgcaaattt
    20281 tactgaaacc ggttttaaac gaattgaatt taaagcatgg ttttggtaaa gttaatgtat
    20341 aaaactaagt tagtattgta ataatattga agattctaac tatacgaagg agaaatgtaa
    20401 ttatggaaca aaattcatat gtaatcatcg acgagactgg tattcacgct agaccagcaa
    20461 caatgttagt acaaacagct tcaaaattcg attctgatat tcaattagaa tataacggta
    20521 agaaagtaaa cttaaaatca atcatgggtg ttatgagcct tggtgttggt aaagatgctg
    20581 aaattacaat ttatgctgac ggtagtgatg aatctgacgc cattcaagca atcagtgacg
    20641 tcttatcaaa agaaggattg actaaataat catgtctaaa ttaattaaag gtattgctgc
    20701 atctgatggt gtcgcaattg ctaaagctta tttattagtt gagccagact taacattcga
    20761 caaaaatgaa aaagtcactg atgttgaagg agaagttgca aagttcaata gcgctatcga
    20821 agcttctaaa gttgagttaa ctaaaattag aaataatgca gaggttcaac taggtgctga
    20881 taaagctgct atctttgatg cacatttatt agttttagat gaccctgaat taattcaacc
    20941 aatccaagat aagattaaaa atgaaaacgc taatgctgct acagcattaa cggatgtaac
    21001 aacacaattt gttacaattt ttgaatctat ggataacgaa tacatgaaag aacgtgcggc
    21061 tgatattcgc gacgtttcta aacgtgtgtt atcacatatt ttaggtgtag aattaccgaa
    21121 tccgagtatg attgatgaaa gcgttgttat tgtagggaat gacttaacgc catctgatac
    21181 tgctcaatta aataaagaat tcgtacaagg ttttgctaca aacattggcg gaagaacaag
    21241 tcactctgca attatgagtc gttctttaga aattccagca attgttggta caaaatcaat
    21301 tactcaagaa gttaaacaag gcgacatgat tatcgtagat ggattaaatg gtgatgtaat
    21361 cgttaatcca actgaagatg agttaatcgc ttatcaagat aaacgtgagc gttattttgc
    21421 tgacaagaaa gaattacaaa aactacgtga tgctgatact gttacagttg atggtgttca
    21481 cgcagagctt gctgcaaata ttggtacacc taatgatttg ccaggtgtta ttgaaaatgg
    21541 tgcacaaggt atcggcttat atagaactga gtttttatat atgggtcgtg accaaatgcc
    21601 tacagaagaa gagcaatttg aagcttataa agaagtatta gaagcaatgg acggtaaacg
    21661 tgttgttgta cgtactttag atataggtgg agataaagaa ttatcatact taaacttgcc
    21721 tgaagaaatg aatccattct taggttaccg tgcgattcgt ttatgccttg cgcaacaaga
    21781 tatttttaga ccacagctac gtgcattatt acgtgcatca gtttatggta agttaaatat
    21841 catgttccca atggttgcaa caattaacga atttagagaa gctaaagcta tattattgga
    21901 agaaaaagaa aaccttaaaa atgaaggtca tgacatttca gatgatatag aattaggaat
    21961 catggtagag atacctgcaa cagcagcatt agctgatgtc tttgctaaag aagtagattt
    22021 cttcagtatc ggtacaaatg atttaattca atacacatta gctgctgacc gtatgtcaga
    22081 gcgtgtatca tatctatacc aaccatataa cccttcaatc ttacgtttag ttaaacaagt
    22141 tattgaagcg tcacataaag aaggtaaatg gataggtatg tgtggtgaaa tggctggaga
    22201 tgaaacagct attccattat tgcttggttt aggtttagat gagttctcta tgagtgcaac
    22261 gtctattctg aaagcaagaa gacaaattaa tggtttaagt aaaaatgaaa tgactgaact
    22321 tgctaaccgt gcagtcgact gtgcaacgca agaagaagtt attgaattag ttaacaacta
    22381 cgtaaaataa ttaattcttt cgaaagacag aattataaac aaacccgaag tattcattgt
    22441 gagtaattat ggttgtccac caaatgtggt gggtatataa tttaaagaac tattttaaa
//
LOCUS       DAHHYJ010000029        20756 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000029 DAHHYJ010000000
VERSION     DAHHYJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20756
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.029"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..852
                     /locus_tag="NBK22_000848"
     CDS             <1..852
                     /locus_tag="NBK22_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414051.1"
                     /translation="FIALYYFNKIGFWYRKEDIYLSSTNINFSLPISIKAITTIQLKH
                     WISNKEEVNLHKPFFYISYSIWFFLGCLIYFSQMAYNQLANAVILILILNTITRDSFS
                     AGTDFFTKSLRFDSDRKSIGLYRMSNTDFKQIYDSKLSLIRFIGFKETILAIILLAIF
                     LNQDIYLYIIGFEIIIINTVIIPNLSLLPSYLSPHFNHQHYSELESFEEQIFLEDTVF
                     DKVKNFLSSSYFLILFVGYLSQRNYIDIMIFIIIFNILALILVSLFIRFSKQKITKSW
                     RKRDLYL"
     gene            890..1354
                     /locus_tag="NBK22_000849"
     CDS             890..1354
                     /locus_tag="NBK22_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HCD2414052.1"
                     /translation="MLIIGGLLGCLVYYLLNIDLGKMIVAQDLNLQEIFFHNLIYFFS
                     AIFGFLTLGLLNIGMLLINSGMLGFFIAHGIYSNQLKSILLVLAPHAIFEILALLIAS
                     TFSLGFLSYLYQRMVKKNKIKNKMFHNFVTTIITVIVLTIIASLLEVYVTIN"
     gene            1366..2088
                     /locus_tag="NBK22_000850"
     CDS             1366..2088
                     /locus_tag="NBK22_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414053.1"
                     /translation="MLVLNNLQKKFTNSKMVLNNVNAKFRSGSINCIVGVNGAGKTTL
                     LNIISSILMPTKGDVYLNSESIFENSTLKKEIFYTPVNPFFYENLSAKDNLYLICSLY
                     NRKIDQITIEKTIKDVGLNIEDLNIPVYNFSSGMKQKLNFASMLLVDSNVLLLDEPFN
                     ALDHVAQKNFTRILKGLVSKEKIIIFTSHLPNTILELSENIYLLKDGCFTDKRKAQSF
                     NQTTLETWLLESQKEVIPYEEK"
     gene            2254..2451
                     /locus_tag="NBK22_000851"
     CDS             2254..2451
                     /locus_tag="NBK22_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2414054.1"
                     /translation="MKNNLKIARVSLSMTQKELANKVGVTRQTISLIEKGVHNPSLSL
                     CKNICSVLNKNLDEIFGEKPQ"
     gene            complement(2961..4553)
                     /locus_tag="NBK22_000852"
     CDS             complement(2961..4553)
                     /locus_tag="NBK22_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414055.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLTNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRSPRPLNNDIL
                     LKFDRVSVDYTSPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKSLRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIKTVVSLLEKVGLDQRFLYRYPHELSGGQRQRVSIARALAVEPKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            4510..5988
                     /locus_tag="NBK22_000853"
     CDS             4510..5988
                     /locus_tag="NBK22_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414056.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKKFYANNSEISEF
                     EQALHQKYKYLSQQKSSTQIHKEALKIFKAQSSNTSSKNIEQAHFSTYFENVLFHKFI
                     MIKVILALPMFILLTFYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNDVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            5994..7157
                     /locus_tag="NBK22_000854"
     CDS             5994..7157
                     /locus_tag="NBK22_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414057.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYQ
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSVVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGICIMFVVLAFNFIGDAGRDALDPRIH"
     gene            7174..8949
                     /locus_tag="NBK22_000855"
     CDS             7174..8949
                     /locus_tag="NBK22_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2414058.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSHDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAIDQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            8987..10993
                     /gene="ggt"
                     /locus_tag="NBK22_000856"
     CDS             8987..10993
                     /gene="ggt"
                     /locus_tag="NBK22_000856"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCD2414059.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHAANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWSEGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTIALEDLANYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HIDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDIEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRIYSSHPNRIEWEPQFSQSTILALIAHGHAME
                     HKPDAYIGDVHGLQVDLNTRDASGGADDTREGTVIGGDVLSIRKQPLPSPKIYDNDTH
                     RVYFNDMQLPLYAEQVRWMHDKYWVDESVVRIIFSEVSAHIEDIRSYEIAGKNYIDIA
                     WLARKKGYQVTLKDDSLYLTDETYHSVKANTNAYYRYDRDSITR"
     gene            complement(11291..12064)
                     /locus_tag="NBK22_000857"
     CDS             complement(11291..12064)
                     /locus_tag="NBK22_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2414060.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELLKEDRMLLKVDKNT
                     HKITGEYDTTTNDRKNATDSTYKSYPVKVVNNKIVFTKDVKDPALKQKIENNQFLIQS
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPRNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKDQIIYKESLTYNKLSEH"
     gene            complement(12253..12879)
                     /locus_tag="NBK22_000858"
     CDS             complement(12253..12879)
                     /locus_tag="NBK22_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCD2414061.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            13088..13666
                     /locus_tag="NBK22_000859"
     CDS             13088..13666
                     /locus_tag="NBK22_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCD2414062.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHLKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRIFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            14050..15147
                     /gene="ugpC"
                     /locus_tag="NBK22_000860"
     CDS             14050..15147
                     /gene="ugpC"
                     /locus_tag="NBK22_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCD2414063.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKIEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            15160..16431
                     /locus_tag="NBK22_000861"
     CDS             15160..16431
                     /locus_tag="NBK22_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD2414064.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDEFKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            16434..17702
                     /locus_tag="NBK22_000862"
     CDS             16434..17702
                     /locus_tag="NBK22_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2414065.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            17704..18543
                     /locus_tag="NBK22_000863"
     CDS             17704..18543
                     /locus_tag="NBK22_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD2414066.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            18617..19693
                     /locus_tag="NBK22_000864"
     CDS             18617..19693
                     /locus_tag="NBK22_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD2414067.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRADSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELENKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            19718..>20756
                     /locus_tag="NBK22_000865"
     CDS             19718..>20756
                     /locus_tag="NBK22_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD2414068.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPQAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAMTAKKAIEAGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
BASE COUNT         7030 a         3115 c         3843 g         6768 t
ORIGIN      
        1 tttatagcgt tatattattt taataaaata gggttttggt atagaaaaga agatatctat
       61 ctatcttcaa caaatattaa tttttcgttg ccaatttcaa taaaagctat aacaacaata
      121 cagctaaaac actggataag taacaaagaa gaagttaatt tacataaacc tttcttctat
      181 attagttatt caatatggtt ctttttaggg tgtttaatct atttttcaca gatggcatat
      241 aatcagttag ctaatgcagt tattttaatt ttgatattaa acactataac tcgtgatagt
      301 ttttctgcgg ggacggattt ttttactaaa tcattacgtt ttgattcaga tagaaaaagt
      361 atcggtttgt ataggatgtc aaacactgat tttaaacaaa tttacgattc taaattatct
      421 ctaattcgct ttattggttt taaagaaaca atacttgcta ttattttatt agcaatattt
      481 ttaaatcaag acatatattt atatataatt ggatttgaaa taataatcat caatacagta
      541 ataattccta atttatcatt attaccttcg tatttatctc cacatttcaa tcatcaacat
      601 tacagtgaat tagagtcttt tgaagaacaa atttttcttg aagatactgt ttttgataaa
      661 gttaagaatt ttttatcgtc atcatatttt ttaattctat ttgtaggtta cttatctcaa
      721 cgtaattata ttgacataat gatttttata attattttca acattttagc cttaatactt
      781 gtttcgttgt ttattaggtt ttctaaacaa aaaataacaa agtcttggag aaagagggat
      841 ttatatttat agtattaaat acaaaaaaat ttttattttt aatatattgg tgttaataat
      901 aggcggatta ttgggatgct tggtttatta cttattaaat attgatttag gtaaaatgat
      961 agttgctcaa gatttaaatt tacaagagat tttctttcat aatttaattt atttcttctc
     1021 tgcaatattt ggttttctga cacttggtct gttaaatata ggtatgttac tcataaattc
     1081 tgggatgtta ggctttttta tagcacatgg aatctatagc aatcaattaa aatctattct
     1141 attagtatta gcgccacacg ctatatttga gattttagca cttttaattg cttcaacatt
     1201 ctcattaggt tttttatcat acctttatca aagaatggta aagaaaaata aaataaaaaa
     1261 taagatgttc cataattttg taacaacaat aataactgtt attgtattaa caataatcgc
     1321 atcattatta gaagtttatg taaccataaa ttaaggaagt gtatcatgct agttttaaat
     1381 aatcttcaaa aaaaatttac taattcaaaa atggttttga ataatgtgaa tgctaagttt
     1441 agaagtggga gcatcaattg catagtggga gtaaatgggg caggtaaaac gactttgtta
     1501 aatattatta gtagtatatt gatgccaaca aaaggtgatg tatatttaaa tagtgaaagt
     1561 atttttgaaa atagcacact aaaaaaagag attttttaca caccagtaaa tccctttttt
     1621 tatgaaaatt taagtgctaa agataatttg tacttaatat gtagcctata taatcggaaa
     1681 atagatcaaa taactattga gaaaactatt aaagatgtag gtttgaatat agaagatcta
     1741 aatatacctg tatataattt ttcaagtggt atgaagcaaa aactaaattt tgcttctatg
     1801 ttattagtag attcaaatgt tttattatta gatgaaccat ttaatgccct tgaccatgtt
     1861 gctcaaaaaa atttcacacg aattttaaag ggattagttt ctaaagaaaa gataataatt
     1921 ttcactagtc atttgcctaa tacaatttta gaactctcag aaaatattta tttgttaaaa
     1981 gatggatgtt ttacagataa acgaaaagca cagtcattta atcaaaccac gttagaaaca
     2041 tggttgttag aaagtcaaaa agaggtgata ccttatgaag aaaaatgatt tgttagtaat
     2101 gagtgtagta tatattttaa cttcagtcat aatgttctac attaatggat taagtaaaca
     2161 attttttaca tatgtagttg gtttcccaat tatttatttt ggttatatat gtctagttaa
     2221 tcttataaaa aaaagaagcc ggaataatta aaagtgaaaa ataatttgaa aatagctaga
     2281 gtttcactat ctatgactca aaaggagcta gcaaataaag ttggagtaac tagacaaaca
     2341 atatctctta tagaaaaggg tgtacataac ccctctctat cactatgtaa aaatatttgt
     2401 tcggtgttga ataaaaattt ggatgagata tttggagaga aaccacaata aaagagctta
     2461 ttgaaagata atctaaaagt cgacaatata tttcttctca gaaactgcta ttattttgaa
     2521 aagaaaacac acgtattata aatttaaaat accgaaatat agcattatta aatcaaaaat
     2581 agaattaaaa aagaaaatta atcgagaaaa gtattaaatg tcatttattt gaatgtgtta
     2641 attatgaaag aaaaagagta agtgacgtta aatatacatt gactaagcta agttgacaca
     2701 ctcaaaaatc tgaatattct aataaaatat aaataatccc ccgcacttca catagtggcg
     2761 ggggatttat tctggcgtca aaatgcccag agtatgggtg tgacccttaa atcatggcaa
     2821 ggcacttaaa acttgatatg gtgccgagcc ctaacttagc acaatatagt tagtatgaca
     2881 aagtcatcta acataacaca tcagaggttg cccgatttat tccagaccat acagtgataa
     2941 agttacacaa cgcatgactt ttatttagca ataactgcta cttctgaaat aagttgcttt
     3001 gcatagtctg attgcggatg tttgataata tcttctgtgt tattcagttc aacgatttcg
     3061 ccatttttca taactgcaac gcgatcacat atttcattga taacacccat gtcatgtgtg
     3121 atgaataaat aagtgatgcc gaagtctaac tgtaattgtt ttaataactc gatgatatct
     3181 ttttgaattg aaacgtctaa agcggacact gcctcgtcgc aaacaatcac tttaggttct
     3241 acagcaagtg ctctcgcgat acttacacgc tgacgttgcc caccagataa ttcgtgtgga
     3301 tagcgatata agaacctttg atctaggcca accttttcta acaacgatac gacagtttta
     3361 ataatgtcat cattatcttt gactttccca tgaatgatta gtggtcgttt aatcacatca
     3421 atgactttaa atcttggatt aatagatgcg aatggatctt gaaaaatcat ttgtatctct
     3481 tgtcgtaaag atttcaattc atcatcttta aataaactta atggtaattc gttataccaa
     3541 ataaagcctt ctgacacttc ctttagaccg acgaccgtct tagctaatgt cgatttccct
     3601 gaccctgatt caccgacaat gcctaatgtt tcgccttttc taatagccaa gttaatatca
     3661 ttaactgctc ggtataggct gccactcggt gatgtgtaat ccacgctcac gcgatcgaat
     3721 tttaataaaa tatcattgtt taacggtctt ggcgaacgcg tttgatgaat atcaggaatc
     3781 gcatctatta agcgttttgt ataggtatgt tgtggcgatt taaaaatact ttcaaccgtg
     3841 ccactttcaa cgacacttcc atctttcatt acaatcacat cgtcgcaaaa ttgatacaca
     3901 gcgcctaaat cgtgagtgat aaaaataata gatgtttctg tgtactcata aagggacttc
     3961 attaactgca gtaattgatt ttgtgtactg gcatctaatg ccgttgttgg ttcatctgcg
     4021 attaaaattt gtggctttaa aatcaatgcc attgctatca tgacacgttg acgcatacca
     4081 ccagaaagtt catgtggata agcatcaaat tgtcgagttg catgttttat acctactttt
     4141 tctaaaatgt ctattgtcat cgactttgct tcagatttag atacacgttt atgttgaaat
     4201 agtacttctg taatttgttt gccgatcgtt aatcttggat ttaacgaaga gagtggatct
     4261 tgaaaaatca ttgaaatatc cttacctcga atttgttgta acgctgaagt tgataaatta
     4321 gttaacgatt gcccattaaa aataatttct cctgttaatg tgtgatctgg ataatctggt
     4381 agtagcccta aaatagattt agcagtaata ctttttcctg atcctgattc accaacaata
     4441 cctaggatat gtttttttcg taattcgaaa gagacgtttt ttaccgcttg aactgtagtt
     4501 tcatcataat tgaattgtac attcagactg ttgacttcta ataaatttga catgatgttg
     4561 tgcctcccta ttcacaaatg tgtaaattta ttctatactt ttgtatatca tagtaatcta
     4621 ctcaagatta ttttacaagg agtgattatc ataattactt atttagaatc ttttaagtcg
     4681 ttgtatcaca aggcatttta caagtttgta ttatcggtat taagcttgcc aatattttta
     4741 tatgcagtga taaagttttt cttttctgct aagagaaaaa aattttacgc taataattct
     4801 gaaatttcag aatttgaaca ggcgttacat caaaaatata aatatttatc gcagcaaaag
     4861 tcatccacac aaatacataa agaagcatta aaaatattca aggcacaaag ttctaatacg
     4921 agttcaaaga atattgaaca agcacatttt tcaacatact ttgaaaatgt attatttcat
     4981 aagttcatca tgatcaaagt gatattggcc ttgccgatgt tcatcttatt gactttttat
     5041 ttacagccat tagtaagata tatttttgag cgaattgtca tggctgtgat tgtcatcatt
     5101 ggtgttattg tcagtgtgtt taccattctg tatttttcac cgcttgatgc ggcttatagc
     5161 atactgggac aaaatgcaac aaaggcacag atacatcaat tcaatgtatt acatcatctt
     5221 aacgaacctt attttattca attgtgggat accatcaagg gtgtttttac ctttgactta
     5281 ggtacgactt acaaagggaa tgatgttgtg actaaagcag ttggcgaaag aattccaatt
     5341 acaataattg tcgcagtatt agcgctaatt gtggcattaa ttattgcgat accaattggt
     5401 attatcagtg cgatgaagcg aaatagttgg cttgatatca cgttaatgat aattgcatta
     5461 attggtttat ctattccaag tttctggcaa gggctattat tcattttagc gttctcattg
     5521 aaattggata ttttgccacc atcttatatg ccagaacatc caatatcatt gattttacct
     5581 gtacttgtca ttggaacaag tattgctgct tctatcacgc gtatgacaag gtcttctgta
     5641 cttgaagtaa tgcgcagcga ttatgtttta actgcttatg caaaaggatt atcgacgaca
     5701 caagtagtta ttaaacatat tttgaaaaat gccattattc caattgtaac gctagttggt
     5761 cttctagtgg cagaattact aggcggttca gcagtgacgg aacaagtatt taacattaat
     5821 ggtatcggtc gttatatcgt ccaaaaacaa ctaatacctg atattccagc agtcatgggt
     5881 ggggtcgtat atatatcaat tgtaatatct ttagcaaact taattattga tatattttat
     5941 gctttaatcg atccaaaatt acgtagtgaa attaacgaaa ggaagtgagg catatggtaa
     6001 aacttacaac aaagatagct tccttaaaac tattcgcaag ttatgctatt gcaacttata
     6061 ttttagttat attaacgagt gcattaaatc tttttaaagg ttatgtggcc gatacgttct
     6121 atattgcaga aacattgcta atcgttttaa ccatcatttt aattatcatc ttaacaacgg
     6181 aacaaacatg gaagcatcat gacctatggc gacgtatcgt cgaagtgttg ttattgttga
     6241 tgacattaac aggcaacgta tttacattat taatgtttgt aagtattaga cgttaccaac
     6301 gtacatcgca aatacatagt tataacgggt gggaatcgtt tatacgaaaa actactagac
     6361 atcgtattgc gattatcggg ttacttattt tagtctacat gctgacatta tcagttgtgt
     6421 cacaatttac atttgatacg acattggcta ctaaaaatca gttcaatgca ctgttacatg
     6481 gaccgagtct agcctatccg tttggtactg atgatttcgg tagagactta tttacacgcg
     6541 tagttgtcgg aacgaagctg acattttcaa tttcaattat ttcagtagtt attgcagtta
     6601 tttttggtgt gttactaggc actatcgcag gttattttaa tcatattgat aatttaataa
     6661 tgcgaatttt agatgtagtg tttgcaattc catcattatt gttagcggtg gcaattattg
     6721 catcatttgg agcaagtatt ccaaatttaa ttattgcttt aagtatcggt aatataccat
     6781 catttgcacg gacaatgcgt gccagtgttt tagaaattaa acgcatggaa tatgtagatg
     6841 cagcacgtat cactggtgaa aacacttgga atatcatatg gcgttatatt ttaccgaatg
     6901 cgattgcgcc tatgattgta cgtttttcat taaatatagg tgtggttgta ttaacaacaa
     6961 gtagtttaag tttcctagga cttggtgttg cacctgatgt agctgaatgg ggcaacattt
     7021 tacgtaccgg tagtaactac ttggaaacgc acagtaattt agctattgta cctggtattt
     7081 gtattatgtt cgtcgtttta gcatttaatt ttataggtga tgcagggcgt gatgcactag
     7141 atccaagaat tcattaaaaa ggtagggata gatgtgaaga aaatcattag tatcgcaatt
     7201 atagttttag cgttggtatt aagtggttgt ggtgtcccta cgaaatcaga agtggctcaa
     7261 aagtcatcga aagttgaagt gaaaggcgag cgaccaacaa tacatttcct aggacaagca
     7321 agttatgaaa atgatatgaa tatcgttaaa gatcaattgg aaaatgcagg atttaacgtg
     7381 aagatgaata tccaaccaga ttatggtagc tatcgtacac aacgtcaagc cggcaattat
     7441 gatatccaaa ttgatgactg gatgacagtg tttggtgacc cgaactatgc tatgacggca
     7501 ttatttagtt ctacaggatc aaatagttta ttgaaagata aacatgtaga ccagttgtta
     7561 aataaagctt ctactcaaaa tgaagcagat gttaaacaaa catataagca aattgaagat
     7621 gaagttgtat ttgataaagg gtatatggcg cctttatatg gatcaaaaaa gaatttagta
     7681 tatgacaata aagtgttaga taaaaatagt gttggattgc caaattcacg tgcattaata
     7741 tggcaacaat ttgattacaa caatagtaga gaacgagata cgcggccact tgtgatgaca
     7801 caacaagatg gtgaaattcc tacattggat ccaatacgtt caattgcgcc gtcagtatat
     7861 tcaattaata tgaatatgta cacaaggtta ttattattag atgaaaatga tcatttaaca
     7921 acgaaaggtt cgttaagtca tgattatgct gtgaataagg acaataaagc gttttatttc
     7981 ttgttaagag atgatgatta ttttgcgaaa gtggtcaatg gacaagcacg taatactgga
     8041 gagcgtgtat cggctgaaga tgttaagttt tctttagata gagcacgtga taaaaagtct
     8101 gtgcctaaca ataatactta caatatgcac aaacatataa atgacatcaa gatattaaaa
     8161 gatgaggaca tcgatcagtt gcgtaaagag aaagacaagg atgataaatc aatctatgat
     8221 aagttgatta aagcttataa cgtcaaatcg ttaacgacag atggtcaaaa agtaaataat
     8281 aaagacggta tttatcaaat tgttaaaatt acgacagatc aatcgatgcc tcgagaggta
     8341 aattacttaa cacactcttc ggcaggcatt ttatctaaaa aatttgttaa tcaagtaaat
     8401 caagaatatc caaaaggata tggggatagc agtacaattc ctgcaaattc agatgggaaa
     8461 aatgcgctgt atgcaagtgg cgcatacatt atgacacaga aaaatgcata tcaagcaacg
     8521 tttcaacgta atccaggatt caacgaaaca gaaaaaggta gttatggacc agctaaaatt
     8581 aaaaatatta cattgaagtt taatggtgat ccgaataatg cattgtcaga acttagaaat
     8641 cattcaattg atatgttggc agatgtgaat caaaaacatt ttgatttaat taagtcggat
     8701 aaaaatttaa gcattattcg caaaaatgga cgcaagtcag tctttttaat gctaaatatt
     8761 aaaaaaggta tatttaagac gcatccaaac ttgagacaag cagtagttaa tgcgatagat
     8821 caggatcaat ttattaagtt ttatcgtggc gataaattta aaattgcatc accgattaca
     8881 ccacttgtcg atactggtaa cgagcaacgt caagatttag aaaaagtaga aaaagccatt
     8941 aatcaataat gttttaaata tttaacagaa agtaggagga tatagtatgg tcattaactt
     9001 aaatgacaaa cagacaaaaa catctaaaga agggttaatt tccgtatcac atcctcttgc
     9061 ggctaaaatt ggtaaggatg tattagatca aggtggcaac gccatggatg cagtgattgc
     9121 aattcaactg gcattgaatg tggtagaacc atttgcatca ggtattggtg gtggcgggta
     9181 tttgctatat tatgagcaaa gtactggcag tataactgcg tttgatgcgc gtgagacagc
     9241 ccctgcacat gtagataaac aattttatct agatgattca ggcgaatata aatcattttt
     9301 tgatatgact acacatggta aaactgtcgc tgtgccagca attccaaagc tgtttgatta
     9361 tattcacaag cgttatgcta agttgtcatt ggaagattta attaatcctg caattgaact
     9421 agctattgaa ggtcatgcag ccaattgggc tactgaaaaa tattcgcgcc agcaacacgc
     9481 acgattgaca aagtatcatg aaacggcaca agtatttacg catgaaaatc aatattggag
     9541 tgaaggtgat tggattgtac aacccgaatt aggtaagaca tttcaaatat taagagaaca
     9601 agggtttaat gcattttata aaggtgacat tgcgaaacaa ttagtcaatg ttgtcaaagc
     9661 atgtggtggg acaatcgctt tagaggatct agccaattat gacattcaga ttaaagcgcc
     9721 aatcagtgcg acatttaaag actatgacat ttattcaatg ggtccatcta gttccggtgg
     9781 tatcacggta attcaaatat tgaagttatt agaacacatc gatttaccat ctatgggtcc
     9841 aagatctgtc gattacttgc atcatttgat acaagcgatg catttagcat atagtgatcg
     9901 tgcgcaatac ttggcggatg ataattttca tgaggtgcct gtacagtcat taattgatga
     9961 cgattattta aaagcacgca gtacgctcat tgatagcaat aaagcaaata ttgatataga
    10021 gcatggtgtt gtgtctgatt gcattagtca tacagatatt gaagaaaatc ataccgaaac
    10081 aactcatttt tgtgtgattg ataaggaagg taatattgct tcatttacga catcaattgg
    10141 tatgatttat ggttcaggta tcacgattcc aggctacggt gtgttattga atacgacaat
    10201 ggatggcttt gatgtagtag atggtggtat taacgaaatt gcaccatata aacgaccact
    10261 aagtaacatg gctccaacga ttgtgatgta tcacgggaag ccgatattaa cagtaggtgc
    10321 acctggtgcc ataagtatca ttgctagtgt tgcgcaaaca ttaatcaatg tattagtgtt
    10381 tggcatggat attcagcagg ctatagatga acctagaatt tatagtagcc atcctaatcg
    10441 cattgaatgg gagcctcaat tttcacaatc tacaatatta gcattgattg cacatggaca
    10501 tgcaatggaa cataaaccag atgcctatat tggagatgta catggactac aggttgactt
    10561 gaatacacgt gacgcgtcgg gaggtgctga tgatacgagg gaaggcacag tgataggtgg
    10621 cgatgtatta tcaattagaa aacaaccatt acctagtccg aaaatatacg ataatgacac
    10681 ccatcgagta tatttcaatg atatgcagtt gccattatat gctgaacaag tgcggtggat
    10741 gcatgacaaa tattgggttg atgaaagtgt tgtcagaatc atcttctctg aagttagtgc
    10801 acatattgaa gatataagaa gttatgaaat cgccggaaaa aattatatag atattgcctg
    10861 gttggcacgg aagaaaggtt atcaagttac cttaaaggat gacagtttat acttaactga
    10921 tgaaacgtat cattcagtga aagcaaacac aaatgcatac tatagatatg atcgagatag
    10981 tatcacaaga taatttatgt atttgatata tagtttgaag tggattttga tttttgttga
    11041 acgaaagggt tcgatttcaa actttagagt gatgcttgag tgtagtaatg ttgaagttgt
    11101 tataagaaac gctagtcgcc tttgtaaaat ataaaacgcc ccacaaattc gtttttgatg
    11161 tacaccacaa tagttagtct aaaatctaac ttgatgtgat tcggtacaat taaatgaatg
    11221 atgtgaggcg tttttataat gtgagatttt aataaatgat ttggttattt ctataattac
    11281 atcataagta ttaatgttca cttaatttat tataagtaag gctttctttg tatataattt
    11341 ggtcttttcc aggacttgaa aaataaaaat aaagtttctg atcttgatga ttattgcctt
    11401 ttaaatcact cattcccttt aattccacct ttgttgatgc atttctggga atattatatc
    11461 tacgttttaa ttgtttaacg tttggattat cattcgacaa cttaccagat aaattataat
    11521 aatcagtggt aggatcatgc gtgactttca aatcattgct atttaaaatg cttgttaaat
    11581 caccactttg aatcaaaaat tgattgtttt cgattttttg tttcagcgcg ggatctttta
    11641 cgtcttttgt gaaaacgatt ttattattaa ctacttttac tggataactt ttgtatgtcg
    11701 agtcagtagc attttttcta tcgtttgtag ttgtgtcata ttcaccagtt attttatgtg
    11761 tgttcttatc tacctttaac aacatacggt cttcttttaa aagctcatct gatccaacaa
    11821 ctgaataaga ggattctata taccatgtgt cttgatcatt attttcataa tggggattat
    11881 cgtgaccatc aatttcataa agcgtttcta agtttttaat aggatacgta cttagtactt
    11941 ttttaagacc atctttcaaa tgaatttgtt cccacttcat tgccaaaaac ataccgccac
    12001 tgactacaat tgaaataata ataattgctg ctaagtttaa ccagaaaatt ttatgtgctt
    12061 tcatacattc ccaccgtttc tcaaaatgct tcattaacac tataataata tattttgaaa
    12121 aatattcaca tcagtattaa agtgaatatc aaattttaaa tttatgaaaa taatagatat
    12181 ttataaaaag cggaaaagag atacaataaa aaactgcatg acgttgagga gtcacacagt
    12241 gtaattaaaa atttaaaatg tagttgctaa tttttcagca ttattaatac tagttgcttt
    12301 aatttcttca gtcttatgag gttcagcatt gtgtccttca ataatgattg tttcatatga
    12361 tggcacacct aagaatgtca taattgttct taaataacgg tcacccattt caaaatcagc
    12421 agcaggtcct tcagtataat atccaccacg tgattgaatg tgtaatactt ttttgtcagt
    12481 taacaaacct tgaggtcctt cagcagaata tttaaaagtt ttacctgcaa ttgaaatagc
    12541 atcaatatat gctttaacta caggtgggaa agaaaggttc cacataggcg ttacaaatac
    12601 atatttatct gcacttaaaa attcttctaa aatgtcactc aatcttgaaa ctttcatttg
    12661 ttcatcatca gttaacgttt cgccattact catttttccc caaccagtta atacatcttt
    12721 gtcaataact ggaatataag tttcaaataa atcaatatgt ttcacttcat catcaggatg
    12781 ttgttgttga tatgtttcga taaatgcttt accagccgcc atagaatttg ataccagttc
    12841 attaaaaggg tgtgctgtaa tatataatac ttttgccatt tgaaaattct cctctgtttc
    12901 tgttattttc ttgagtataa ttattatatt agatataaaa tttgatatca ataaaaatat
    12961 tcaaattacc atcattttct tcatctatat ttggcagtac tactaaagta tgagtgcatt
    13021 taattatgaa atagttgatt tagaatatat acttaatacc caaaatatat gaaggatgga
    13081 tgccactatg acaaagcgac caaaacgtat tttggcaaca attatcattt ttctttcact
    13141 attatttacg attatttata tagatgacat tcaaaaatgg tttaaccaat ataccgataa
    13201 attgacacaa aatcataaag gacaaggaca cttaaaatgg gaagactttt ttagagggag
    13261 taggattact gagacttttg gcaaatatca acattcacca tttgatggta agcattatgg
    13321 cattgatttt gcattgccaa aaggtacacc aattaaagcg ccgacgaatg gtaaagtaac
    13381 acgtatcttt aataatgaat taggcggcaa ggtattacag attgccgaag acaatggaga
    13441 atatcaccag tggtatctac acttagacaa atataatgtc aaagtaggtg atcgagttaa
    13501 agcaggtgat attattgcat attcaggcaa tacaggtaaa caaacgacag gcgcacattt
    13561 acattttcaa agaatgaagg gtggcgtagg taatgcatat gcagaagatc ctaaaccgtt
    13621 tatcgatcag ttacctgatg gggaacgtag cctatatgat ttgtagttat agaagagtgc
    13681 ccgcagtcta aaaaattaag caatcattgt gtgagtatga tacttacata atggttgctt
    13741 ttttcaatga aaatcgtaat gctaagtcat acttgtttga tttagatatt acttaaaatg
    13801 taagacaagg ttgttagcat tggcagtgaa atatcgcaca taaaaaaaca ttattgtcac
    13861 actagaaaat agttgtgcac tatatcaatt ttctgtataa aagtttaatt ctgacagtaa
    13921 tgtaaacgtt tacaatttat gattgacatt aataatgact gaatatatga tttatgtaag
    13981 tatttgtgca aacgttttca caaagtgtat tgcacaatca aactgtaaac aaagtatggg
    14041 aggcataaca tggcagaact aaagttagag catattaaaa agacgtatga taacaacaat
    14101 actgtagtga aagattttaa tctacatatt actgacaaag aattcattgt atttgttgga
    14161 ccatcgggat gtggtaaatc aacaacatta cgaatggttg ctggactaga gtctatcaca
    14221 tctggagatt tttatattga tggggaacgc atgaacgatg ttgaaccaaa gaatagagat
    14281 attgcgatgg tatttcaaaa ctatgcatta tatccacata tgactgtttt tgaaaatatg
    14341 gcatttgggc taaagctacg taaagtaaat aaaaaagaga ttgaacaaaa agttaatgaa
    14401 gcagctgaaa tattaggatt aactgagtat cttggtcgta aaccaaaagc gttatctggc
    14461 ggacagcgtc aacgtgttgc tttgggcaga gctattgtta gggatgcgaa agtcttttta
    14521 atggatgaac cattatcgaa tcttgatgcg aagcttcgag tacaaatgcg cacagaaata
    14581 ttgaaattac ataagcgact taatactacg acaatttatg ttacacatga tcaaactgaa
    14641 gcattgacga tggctagtcg aattgttgtt ttgaaagatg gcgacattat gcaagtcggc
    14701 acacctagag aaatatatga tgcccctaat tgcatatttg tggcgcaatt tatcggctca
    14761 ccagcaatga atatgttgaa tgctacagtt gaaatggacg gattgaaggt aggaacacac
    14821 cattttaaat tacataataa aaaaattgaa aagttaaaag ctgctggcta cttagacaag
    14881 gaaattattt taggtattcg agctgaagac attcatgaag aaccaatatt tattcaaact
    14941 tctccagaga cacaatttga atctgaagta gttgtatccg aactgttagg ttcagaaatt
    15001 atggtacata gcacattcca aggaatggaa ttgatttcta aattagattc aagaacacaa
    15061 gtgatggcga acgacaagat tacactagca tttgatatga ataagtgtca cttttttgat
    15121 gaaaaaacag gaaatcgtat cgtctaaggg ggagtattca tgtctaaaat tttaaaatgt
    15181 atcacgttag ccgtggtaat gttattaatc gtaactgcat gtggccctaa tcgttcgaaa
    15241 gaagatattg ataaagcatt gaataaagat aattctaaag acaagcctaa ccaacttacg
    15301 atgtgggtgg atggcgacaa gcaaatggcg ttttataaaa aaattacgga tcaatatact
    15361 aaaaaaaccg gcatcaaagt aaagcttgta aatattggtc aaaatgatca actagaaaat
    15421 atttcactag acgctcctgc aggaaaaggt ccagatatct ttttcttagc acatgataat
    15481 actggaagtg cctatctaca aggcttagct gctgaaatca aattatcaaa agatgagttt
    15541 aaaggtttca ataagcaagc acttaaagcg atgaattatg acaataagca actagcattg
    15601 ccagctatcg tagaaacaac cgcacttttc tataataaaa aattagtgaa aaatgcaccg
    15661 caaacgttag aagaagttga agctaatgct gccaagctaa ctgatagtaa aaagaaacaa
    15721 tacggtatgt tatttgatgc taaaaatttc tattttaatt atccgttttt attcggcaat
    15781 gatgattata ttttcaagaa aaatggcagt gaatatgata ttcatcagct aggactaaat
    15841 tcaaaacatg tcgtcaagaa tgctgaacga ttacaaaaat ggtacgacaa agggtatctt
    15901 cctaaggcag caacacatga tgtcatgatt ggtcttttta aagaaggaaa agtaggacaa
    15961 tttgtcactg gaccgtggaa cattaatgaa tatcaagaaa cgtttggtaa agatttagga
    16021 gtaacaacat tgcctacaga tggtggcaaa cctatgaaac catttctcgg tgtacgtggt
    16081 tggtatttat ctgaatatag taaacataag tattgggcta aagatttaat gctgtatatc
    16141 actagcaaag atacattaca aaaatataca gatgaaatga gcgaaattac tggacgtgtt
    16201 gacgtgaaat catctaatcc aaatttaaaa gtgtttgaaa agcaagcacg tcatgctgaa
    16261 ccgatgccta atattcctga aatgcgacaa gtttgggagc cgatgggcaa tgcaagcata
    16321 tttatctcaa atggtaagaa tcctaaacaa gcgttagatg aggcgacgaa tgatataacg
    16381 caaaatatta agattcttca tccatcacaa aatgataaga aaggagatta gttatgacga
    16441 aacgtaaccc taaattagcg gcattgttat ctgtaatacc tggtttggga cagttttata
    16501 ataaaagacc tattaaaggg acgatatttt ttatcttttt catcagtttt atttctgttt
    16561 tttatagctt tttaaatatt ggtttttggg gattgttcac attagggaca gtacctaagt
    16621 tagacgattc tcgtgtctta cttgcacaag gtattatttc tatcttactc gttgcttttg
    16681 caatcatgct atatgtcatt aatattttag atgcatatcg taatgctgaa cgatttaatc
    16741 gcaatgagga aataaaggat ccgaaggcac gtatggttgc aacatgggac aagacgttcc
    16801 catacttact aatctcacca ggtacattct tattgatatt tgtagttgta tttccattaa
    16861 tatttatgtt tggagtagca tttacaaatt acaatttata caacgcgcct ccgagacaca
    16921 cattagaatg ggttggttta gataacttta aaacgttatt cacaattggc gtttggcgta
    16981 aaacattttt cagtgttatt acttggacat tagtatggac gcttgttgca acgacacttc
    17041 aaattgcatt agggctgttt ttggcaatta ttgtaaatca ccctgtcgtc aaaggtaaga
    17101 aatttatccg tactgtgtta atcctacctt gggctgtacc atcatttgtg acaattttaa
    17161 tatttgtagc gttatttaat gatgaatttg gtgcgataaa taatgatatt ttgcaacctt
    17221 tattaggtgt agcaccagca tggttaagtg atccgttttg ggcaaaagtg gcattaatcg
    17281 gcattcaagt atggcttgga ttcccatttg tctttgcact gttcactgga gtactgcaaa
    17341 gtatttcatc agattggtac gaagcagcag atatggatgg tgcgtctagt tggcaaaagt
    17401 ttagaaacat cacattcccg catgtcattt acgccacagc gccattgtta attatgcaat
    17461 atgcaggtaa tttcaataat tttaatctta tttatctatt taataaaggc ggtccaccag
    17521 tgtcagggca gaatgctggt agtacagata tcttgatatc ttgggtgtat aatctgacat
    17581 ttgagtttaa caacttcaac atgggtgcag ttgtgtcatt aattattgga tttattgttg
    17641 ctattgtcgc atttattcaa ttcagacgta caagtacgtt taaagatgag ggaggtttat
    17701 aagatgacaa agaagaaaaa catattaaaa gcaatcggta tttacagttt tatagcgatg
    17761 atgtttgtca tcattttata tccactactg tggacatttg gcatttccct taatccaggt
    17821 acgaacttgt atggtgccaa aatgatacca gacaatgcaa catttaaaaa ttatgcattc
    17881 ttactattcg atgacagtag tcaatacctg acttggtata aaaatacgct tatcgtagca
    17941 tctgcaaatg cactgtttag tgtgatattt gtcacgttaa cagcatatgc tttttctaga
    18001 tatcgctttg ttggtcgtaa atacgggctg attacatttt tgattttaca aatgttccct
    18061 gtattaatgg caatggtcgc aatctatatt ttgctaaata caattggatt attagattct
    18121 ttatttggac taacactggt atatattggt ggatcaatac cgatgaatgc ctttttagtg
    18181 aaaggttact tcgatacgat tccaaaagaa cttgatgaat ctgccaaaat tgatggtgca
    18241 gggcatatgc gtattttctt acaaattatg cttccattag ctaagccgat tttagcagtt
    18301 gttgctttgt tcaattttat ggggccattt atggacttta tattacctaa aatactatta
    18361 agaagtcctg aaaaattcac attagcagtt ggattgttca actttattaa tgataagtat
    18421 gcaaataatt tcacagtgtt tgcagcaggg gcaattatga ttgcagtacc tatagcaatc
    18481 gtattcttgt tcttgcaacg ctatttagta tcaggtttaa caacaggtgc gacaaaaggt
    18541 tagtttgaaa ttaggagtgg ggcagaattg ataaagaacc actaatgacg ataaagatta
    18601 aaaggaggac gttatgatga cgattaaagt tggaatcatt gggtgtggtg gtattgcgaa
    18661 tggcaagcac atgccaagtt tacaaaaagt tgaaaatgtt gaaatgatcg cattttgtga
    18721 cgtagacatt tcgaaagcag cgagtgcggc agaagcatac ggaactgaca atgcaaaggt
    18781 ttatgatgat tacaaagcat tgttaaaaga tgacacgatt gatgttatcc atgtttgtac
    18841 gccaaatgac tcgcattgtg aaattactgt agcagggttg catgctggta aacatgtgat
    18901 gtgtgaaaaa ccaatggcta aaacgacagc agaagctcaa aaaatgatag atacagctaa
    18961 atcaacaggt aaaaaattaa caataggtta tcaaaatcgt ttccgagcag atagtcaatt
    19021 tttacatcaa gcagcgcaac gtggcgactt aggagacatt tacttcggaa aggcacatgc
    19081 cattcgtcgt cgagcagtac caacatgggg tgtctttcta gacgaagaag ctcaaggtgg
    19141 aggaccatta atcgatatcg gtacacacgc tttagattta acgttatgga tgatggataa
    19201 ttatgaacca gaatcagtga tgggttcaac attccataaa ttaaataaac agcatcatgc
    19261 ggcaaacgct tggggttcat ggaatccaga tgaatttaca gttgaagatt ctgcgtttgg
    19321 atttattaaa atgaagaatg gagcgacgat cattttagaa tccgcttggg cgattaattc
    19381 tttagaagtg gatgaggcaa aatgttcatt atcaggaact aaagcaggtg ctgatatgaa
    19441 agatggtcta cgtattcatg gtgaagacat gggtacactt tataccaaac acgttgaatt
    19501 ggaaaacaaa ggcgtcgact tttatgaagg taatgaagtg gatgaagctg aagaagaagc
    19561 aaaagcttgg attgatgcag ttgtaaatga tactgaacca gttgtgaaac cggaacaagc
    19621 aatggtagtt acaaaaattc ttgaagcgat ttatcagtct gcaaaatcag gcaaagcaat
    19681 ttactttgaa taacatcata cggtaaggag gcacatcatg acaaaattaa aagttggtgt
    19741 gataggtgtt ggtggcattg cacaagaccg tcatattcca gcattgctga aactcaaaga
    19801 cacagtctca ttagttgcag tacaagatat taatacagtt cagatgattg atgttgcgaa
    19861 gcgctttaat atacctcagg cagttgagac acctagcgag ctgtttaaac ttgttgatgc
    19921 ggtggtcatt tgtacaccca ataaattcca tgctgatctt tctatagaag cattgaacca
    19981 tggtgtccat gtcttatgcg aaaagccaat ggcaatgacg acggaagagt gtgatcgcat
    20041 gattgaagcg gctaataaaa atcacaaatt attaactgtc gcttatcatt atcgtcacac
    20101 agatgtcgct atgactgcta aaaaagcaat tgaagcaggt gtggttggta aaccattagt
    20161 agcacgtgta caagcgatgc gtaggcgtaa agtacctggg tggggcgttt ttaccaataa
    20221 agcgttgcaa ggtggcggta gtttaatcga ttatggttgc cacttgttag acttatcttt
    20281 gtggctatta ggtaaagata tggtgccgca tgaagtgcta ggaaaaacat ataatcaatt
    20341 gagcaaacaa ccgaatcaaa ttaatgattg gggaacattt gatcacacta aatttgatgt
    20401 cgatgatcat gttactagtt atatgacatt tgccaatcga gcaagcatgc agtttgaatg
    20461 ttcgtggtct gcaaatataa aggaagataa ggtacacgtt agtttatcag gagaagatgg
    20521 cggtatcaat ttatttccat ttgaaatata tgagccccgc tttggaacta tttttgaaag
    20581 caaagctaat gttgagcata acgaagacat tgctggtgag agacaggcgc gtaactttgt
    20641 caatgcgtgt ttaggtatag aagagattgt ggtgaaaccg gaagaagcac gcaatgtaaa
    20701 tgcccttata gaagcgattt atcgtagcga tcttgataac aagagcatac aacttt
//
LOCUS       DAHHYJ010000030        20751 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000030 DAHHYJ010000000
VERSION     DAHHYJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20751
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.030"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            67..1593
                     /gene="spa"
                     /locus_tag="NBK22_000866"
     CDS             67..1593
                     /gene="spa"
                     /locus_tag="NBK22_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCD2414069.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKEDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     EQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNNKP
                     GKEDNNKPGKEDNNKPGKEDGNKPGKEDNNKPGKEDGNKPGKEDNNKPGKEDGNKPGK
                     EDGNKPGKEDGNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNMIK
                     PGQELVVDKKQPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL
                     "
     gene            complement(1922..3514)
                     /locus_tag="NBK22_000867"
     CDS             complement(1922..3514)
                     /locus_tag="NBK22_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD2414070.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFVIDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLKESKHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVLKMPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLEWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICIWTFILTLIF"
     gene            complement(3776..4249)
                     /locus_tag="NBK22_000868"
     CDS             complement(3776..4249)
                     /locus_tag="NBK22_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCD2414071.1"
                     /translation="MSKIRSFTILSLLIYLAMMCYTVVTYSKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            complement(4439..5836)
                     /locus_tag="NBK22_000869"
     CDS             complement(4439..5836)
                     /locus_tag="NBK22_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD2414072.1"
                     /translation="MTKYKDIASDIRDKIIAGDWFYGMKIPPQRQLAIQYNVNRVTII
                     KSIELLEAEGFIYTKVGSGTYVNDYLNEAHITNKWSEMMLWSSRQRSQYTVQLINKIE
                     TDDSYIHISKGELGQPLMPHIQLKKAMSNTASHIEDLSFGYNNGYGYIKLRDIIVERM
                     SKQGINVGRENVMITSGALHAIQLLSIGFLGQDAIIISNTPSYIHSTNVFEQLNFRHI
                     DVPYNQINEIDTIIDRFINFKNKAIYIEPRFNNPTGRSLTNEQKQNIITYSERHNIPI
                     IEDDIFRDIFFSDPTPAIKTYDKLGKVIHISSFSKTIAPAIRIGWIVASEKIIEQLAD
                     VRMQIDYGSSILSQMVVYEMLKNKSYDKHLVKLRYVLKDKRDFMLNILNNLFKDIAHW
                     EVPSGGYFVWLVFKIDIDIKYLFYELLSKEKILINPGYIYGSKEKSIRLSFAFESNEN
                     IKHALYKIYTYVKKG"
     gene            5950..6714
                     /locus_tag="NBK22_000870"
     CDS             5950..6714
                     /locus_tag="NBK22_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HCD2414073.1"
                     /translation="MNKLREYGFERYDLLKELNYEGIEFDYLELLNYFSDLPIDEYAP
                     TLNRYRRYSRALILPGNDQIFWLPIQKINNSEYSVYFQGKFNPEFPESYRKFRPIDQH
                     IQNNKLLNKIISSNFKKTFWNEEDKILPIHVGVHFVKLFVNDAFKKATSSPDCLHQDG
                     EPFTFAHLIERKNVKGGTNVIASPQVVGKKPHEINNEDIIEIFELSKALESYGVHDTS
                     VSHYVSAIEQDNKSNPATRSVILIDYQQTIVANINE"
     gene            6707..7579
                     /locus_tag="NBK22_000871"
     CDS             6707..7579
                     /locus_tag="NBK22_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD2414074.1"
                     /translation="MNKLRDTTFLSYLFTIILWGSAFPMIKIALNDFSAESLSAFRLI
                     LATIILLPFVIIKKLPTPELRDIPVIFILGFCEFVIYHTALNFGETLISAGISGILVS
                     TTPIFSSALAYIFLKEHFSKWNWLSSLVAFIGISIISISKDDYTTINVLGVLIILLAS
                     FSESLYFTFQKKYIEKYGFIAFTLYTIMASSPFMLIFIPEIINDIHGATFTSIVSVLY
                     LAIFPTIIPYVLLAYIVKSVGVSDATMSLYLTPIVSLLLSYLLLDELPTTLAIIGGII
                     TLLGVSLSNFFQNT"
     gene            7753..9528
                     /locus_tag="NBK22_000872"
     CDS             7753..9528
                     /locus_tag="NBK22_000872"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCD2414075.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKTAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIDRNGNAE
                     SIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPKKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFT
                     INRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVSTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIESLLKEHKLL"
     gene            9853..11514
                     /locus_tag="NBK22_000873"
     CDS             9853..11514
                     /locus_tag="NBK22_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCD2414076.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSSNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKIDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(12001..13389)
                     /locus_tag="NBK22_000874"
     CDS             complement(12001..13389)
                     /locus_tag="NBK22_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2414077.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGTDM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVFSMLLIKGTPETKSEVINTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLKVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(13391..14569)
                     /locus_tag="NBK22_000875"
     CDS             complement(13391..14569)
                     /locus_tag="NBK22_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD2414078.1"
                     /translation="MNQQLIEALKSKEDKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGVLDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIETEIKR
                     LSKGLEALNGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEADLEFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            complement(14720..16957)
                     /locus_tag="NBK22_000876"
     CDS             complement(14720..16957)
                     /locus_tag="NBK22_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2414079.1"
                     /translation="MQRDYLIRVETESMSDFKRLNSLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDAHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFTESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHDYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFKPNNQQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFHVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATQRHLPLLKRLEPHIDHFVFDANSNDAVDFNKMNDDEFKTASQMIINK
                     TNYLIDLMHRHNLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSTKVEGIGY
                     WLNYDLHVSHCRNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINDLEDGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITDTLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            complement(17051..17821)
                     /locus_tag="NBK22_000877"
     CDS             complement(17051..17821)
                     /locus_tag="NBK22_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2414080.1"
                     /translation="MIKRVNKLVLGISFLFLIISIFAGCGTGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIVRSSMSIQSNGKDMNIKGMVLYMNRNTRTT
                     NGYYYVDVIEREDKGIHRDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKDLSKYKDRDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYKIPTNKAPKLLLKGSG
                     DLKGSSVGYKDIEFTFVEKKGENTFFTDSLHLEPSEDK"
     gene            complement(17873..18643)
                     /locus_tag="NBK22_000878"
     CDS             complement(17873..18643)
                     /locus_tag="NBK22_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2414081.1"
                     /translation="MIKRVNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWIIGSEMATQNKGEALKVKGMVLYMNRNTRSA
                     KGFYYVNSVKKDEDGRPQDNKIEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYG
                     SFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTNKSPKLLLKGSG
                     DLKGSSVGYKDIEFTFVEKKGENIYFSDSLHLEPSEDK"
     gene            complement(18695..19465)
                     /locus_tag="NBK22_000879"
     CDS             complement(18695..19465)
                     /locus_tag="NBK22_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2414082.1"
                     /translation="MMKRLNKLVLGIIFLFLVISITAGCGIGKEAEVKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVIQPNNEDMVAKGMVLYMNRNTKTT
                     NGYYYVDVTKDEDEGKPHDNEKRYPVKMVDNKIIPTKEIKDEKIKKEIENFKFFVQYG
                     DFKNLKNYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYDIPTSKAPKLLLKGSG
                     NLKGSSVGYKDIEFTFVEKKEENIYFSDSLDYKKSGDV"
     gene            complement(19686..20672)
                     /locus_tag="NBK22_000880"
     CDS             complement(19686..20672)
                     /locus_tag="NBK22_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCD2414083.1"
                     /translation="MKKCIKTLFLSIILVVMSGWYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLKDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWPGYDDIVSEIIDSNK"
BASE COUNT         6876 a         3778 c         3076 g         7021 t
ORIGIN      
        1 ttagtattgc aatacataat tcgttatatt atgatgactt tacaaataca tacagggggt
       61 attaatttga aaaagaaaaa catttattca attcgtaaac taggtgtagg tattgcatct
      121 gtaactttag gtacattact tatatctggt ggcgtaacac ctgctgcaaa tgctgcgcaa
      181 cacgatgaag ctcaacaaaa tgctttttat caagtcttaa atatgcctaa cttaaatgct
      241 gatcaacgca atggttttat ccaaagcctt aaagatgatc caagccaaag tgctaacgtt
      301 ttaggtgaag ctcaaaaact taatgactct caagctccaa aagctgatgc gcaacaaaat
      361 aacttcaaca aagatcaaca aagcgccttc tatgaaatct tgaacatgcc taacttaaac
      421 gaagcgcaac gtaacggctt cattcaaagt cttaaagaag acccaagcca aagcactaac
      481 gttttaggtg aagctaaaaa attaaacgaa tctcaagcac cgaaagctga taacaatttc
      541 aacaaagaac aacaaaatgc tttctatgaa atcttgaata tgcctaactt aaacgaagaa
      601 caacgcaatg gtttcatcca aagcttaaaa gatgacccaa gccaaagtgc taacctattg
      661 tcagaagcta aaaagttaaa tgaatctcaa gcaccgaaag cggataacaa attcaacaaa
      721 gaacaacaaa atgctttcta tgaaatctta catttaccta acttaaacga agaacaacgc
      781 aatggtttca tccaaagcct aaaagatgac ccaagccaaa gcgctaacct tttagcagaa
      841 gctaaaaagc taaatgatgc tcaagcacca aaagctgaca acaaattcaa caaagaacaa
      901 caaaatgctt tctatgaaat tttacattta cctaacttaa ctgaagaaca acgtaacggc
      961 ttcatccaaa gccttaaaga cgatccttca gtgagcaaag aaattttagc agaagctaaa
     1021 aagctaaacg atgctcaagc accaaaagag gaagacaata acaagcctgg taaagaagac
     1081 aacaacaagc ctggcaaaga agacaacaac aagcctggta aagaagacgg caacaagcct
     1141 ggtaaagaag acaacaacaa acctggcaaa gaagacggca acaagcctgg taaagaagac
     1201 aacaacaagc ctggtaaaga agacggcaac aagcctggta aagaagacgg caacaagcct
     1261 ggtaaagaag acggcaacaa acctggtaaa gaagacggca acggagtaca tgtcgttaaa
     1321 cctggtgata cagtaaatga cattgcaaaa gcaaacggca ctactgctga caaaattgct
     1381 gcagataaca aattagctga taaaaacatg atcaaacctg gtcaagaact tgttgttgat
     1441 aagaagcaac cagcaaacca tgcagatgct aacaaagctc aagcattacc agaaactggt
     1501 gaagaaaatc cattcatcgg tacaactgta tttggtggat tatcattagc gttaggtgca
     1561 gcgttattag ctggacgtcg tcgtgaacta taaaaacaaa caatacacaa cgatagatat
     1621 cattttatcc aaaccaattt taacttatat acgttgatta acacattctt atttgaaatg
     1681 ataagaatca tctaaatgca ctagcaacat cttttgttgc tcagtgcatt ttttatttta
     1741 cttacttttc taaacaactt ctgaaacgcc tcaacacttt ctactctaat tacatatacg
     1801 acatttttag acattaaaaa atcgaactag acaagatgct caatgcattt cgtactagtt
     1861 cgattcatga ataattagat ttaaaatgtc atttgaatcc aagtgacaac attatttata
     1921 tttagaatat taacgttaat ataaacgtcc aaatacaaat aaaggcaaca aatataatac
     1981 tgtattttag cgtcattttt aataatgcag attcttcacc aactttttta acagctgcag
     2041 tcgcaatggc aattgattgt ggtgaaataa gtttcgctgc tacaccacct gcagtgttag
     2101 ctgccacaag taatgaaccg cttgttgaaa tttgttgtgc cactgtcgct tgaataggtg
     2161 cgaataaagt attgttattt acaactgaac cagtcataaa cacaccaatc caacctaata
     2221 ctggagagaa taatgggaaa attgctccag ctttagcaat accttgtcca attgctacag
     2281 tcaaaccacc gtatgtcata actttagcaa tagctagaat cgctgaaatt gtaaggatcg
     2341 gtaaccataa ttctttaatt gcttcgacca ataaagcacc tgcacttttc cattctaact
     2401 tcgtaattaa aattgtaata attactgtta ataaaatcgc tgtcccagtt gcaccaatta
     2461 aatcgagacg caacgcaatt cctttaggcg ataaatcact cacagtattt ggaattggca
     2521 tttttaatac taaactttca agcgcacctc caggttggaa taatttttta aagaatggtg
     2581 cactccatac taatacaaag gcagttaaaa tgacgaacgg actccaagca aagacgattt
     2641 ctttaggtgt acgcttttga attttatgtt ttgattcttt caacctgaaa atgtttttcg
     2701 gtttaaattt acgacaaaca aatgctaaca ccaccattgt tgctagtgat ggaataatgt
     2761 ctgctagttc tggaccatgg aatattgtta ataataattg taatccagta tatgtaccac
     2821 tcactgttaa gatgacaggt aaaatttctt taataccttt catgccatct ataacgaata
     2881 ctaaaacaaa tgggataata aagtttaaaa ttggaagtgt taatgctgag tatctcgcaa
     2941 catctaatgt tgtaacgcct ccacttaagt taaacgtatc aataatacta actggtaaac
     3001 caattgcacc aaaggcaccc gccgcaccat tagcaattaa acataacatc gctgctttta
     3061 atggttcaaa tccaagttga attaataata ctgcacaaat cgcaattggc acaccaaatc
     3121 ctgctgcacc ttctaaaaat gcgttgaaac aaaatccaat taataatagt tggattcttt
     3181 ggtcctctga aatactagca atactatctt gaataataga aaattgtcct gttttaatag
     3241 aaactttata taaccaaact gccattaaaa cgatatatcc tattggaaaa ataccggcaa
     3301 caacgccttc tgtaatcgca cctgctgata cacgcgctgg taattcaaat acaaataaag
     3361 ccacaatcaa tgtaacaacc aaagttgtca atgctgcata aatgcctttc attttaaaaa
     3421 cggttaagca taataaaaat aaaataatag gtactgctgc aactaaggct gataatccga
     3481 cattatcgaa tggatttaca gtaagtagtg tcataatgac tccctctctt tatataaaat
     3541 atttatcatt ctgattaatc tacaacctat ttcaacttat attttgcgat gatcacatat
     3601 ttaaaatgta acactcctat atgtgacagg caatcgaatt tttacaaaaa gttcacaaaa
     3661 tatacacaat atttaactat aataaataat atatcatttt aattataaat actagatatt
     3721 atttataata atctcaggaa ttcgcttcaa aactgcatca tgagagttta tatttttatt
     3781 gagaatctct cattttatga attgtaggaa gtaaacaaaa tatgacaagc gtcaaaccaa
     3841 tgataatgat aaatatcata ttaaaccata gtaaattgaa ttgatgatgg tgttgtattt
     3901 gccaaatttc taatactgtg aagatagaca tatagctcat aatctctaaa tttaacgtac
     3961 ttaaaaataa tttaatcgct cgaatttcac gtggtgtatg attataatgt gaccatttgg
     4021 caaatcgttc atagtatcta cctgcaatga aaaatataag ccaaatcact ataaatgcgc
     4081 tattaatcaa aagcagcacc catttatcag caaaattatc agcatcccct gctaaattat
     4141 aatgaatagg tactttggtt ggtaattttg aataggtcac tactgtatag cacatcatag
     4201 ctaagtaaat aagtagactt aatattgtaa aagacctgat tttagacatt ctatcgcctc
     4261 tttctttaca ttttgtgtat aacactctgc ctattttacc ttttaattac attaccccaa
     4321 acgattaaac aatatgtaat gatactataa ttgcgtcagg agtatccgct tgttaaatgt
     4381 gcatagctta tatttagctg tttaacatgc cacataatga ttcgaattat tgttttaatt
     4441 aacccttttt cacatatgta taaattttat agagcgcatg cttaatattt tcatttgatt
     4501 caaaggcaaa agatagcctt atactctttt ctttactgcc ataaatgtaa cccggattga
     4561 ttaatatttt ttctttactt aacaattcgt aaaataaata tttaatatct atatctattt
     4621 taaagactaa ccatacaaaa taacctccac ttggaacctc ccaatgtgct atatccttaa
     4681 ataaattatt gaggatgttt aacataaagt ctcgtttatc ttttaaaaca taccttaact
     4741 ttactaagtg tttatcataa gacttatttt tcaacatctc atatacaacc atttgtgaca
     4801 aaatactgga tccatagtca atttgcattc ttacatctgc caattgctct attatttttt
     4861 cagaagcaac aatccaacct attcttattg ctggtgcaat cgtttttgaa aaactgctta
     4921 tatgtataac ttttcccaat ttatcataag ttttgatagc aggagttgga tcgctaaaga
     4981 aaatatctct aaagatatca tcttcaatga taggtatatt atgtctttcg ctataagtaa
     5041 ttatattttg cttttgctca ttcgttaaag aacgacctgt cgggttatta aacctaggtt
     5101 ctatataaat cgctttattt ttaaaattaa taaatctatc aatgatggta tcaatttcat
     5161 taatttgatt ataaggaaca tcaatatgtc taaaattcaa ttgctcaaaa acatttgtag
     5221 agtgaatata tgatggtgta ttcgaaatta ttatggcatc ttgacctaaa aacccaatag
     5281 ataaaagttg aatggcatgt aaagcgcctg aagtgatcat tacattttct ctacctacat
     5341 ttataccttg ctttgacatt cgttcaacga tgatatctct taacttgata taaccatagc
     5401 cattattata accaaaagat aagtcttcga tatgactggc tgtattagac atggcttttt
     5461 tcaattgaat atgtggcatt aatggttgac ccaattcacc tttacttata tgtatatacg
     5521 agtcatctgt ctcaatttta ttaattaatt gcaccgtata ctgacttctt tgtcgagagg
     5581 accataacat catttcagac catttatttg taatatgtgc ttcgttcaaa tagtcattaa
     5641 catatgttcc actccccact ttagtataga taaatccttc agcctctaat aactcaatac
     5701 ttttaataat cgtcactcta tttacgttgt attgtatcgc caactgcctc tgtggaggta
     5761 tcttcattcc ataaaaccaa tcccctgcga ttattttatc tctgatgtcg ctagcaatat
     5821 ctttatattt cgtcatcttc tccccccttt ttataaattg gataggtaaa tccaatcaaa
     5881 attggttgta ctcattttaa atcatatttt ttaatataga aacacataat taaaggagtg
     5941 atgatattaa tgaataagct tcgagaatat ggattcgaaa gatatgattt attaaaagaa
     6001 ttaaactatg aaggcataga atttgattac ttagaactat tgaattattt ttcagattta
     6061 cctattgatg aatatgctcc aacgctaaat agatatagac gctattcaag agcattaatt
     6121 ttacctggta atgatcaaat tttttggctc cctatacaaa aaataaataa tagtgagtat
     6181 tctgtatatt tccaaggaaa gtttaatcct gaatttccag aatcatatcg aaagtttcgc
     6241 cctatagatc aacacattca aaataataaa cttttaaata aaattatctc ttcaaacttc
     6301 aaaaaaacat tttggaatga agaagataaa atacttccaa tccatgtcgg agttcatttc
     6361 gtgaaattat tcgtaaatga tgcttttaaa aaagctactt cgtctcccga ttgtttgcat
     6421 caagatggag aaccatttac atttgctcac ttgattgaga gaaagaatgt taaaggagga
     6481 acaaatgtaa tagcttcacc tcaagtagta ggtaaaaaac cacatgaaat aaataacgaa
     6541 gacataattg aaattttcga actatcaaaa gctttagaat cttatggtgt tcatgataca
     6601 tctgtaagtc attatgtaag tgcaatagaa caagataata aatctaatcc tgccacacga
     6661 tctgtaatcc ttatcgacta ccaacaaact attgtagcta atattaatga ataagcttcg
     6721 agacactact tttctatcat atttatttac tatcatattg tggggatctg catttccaat
     6781 gataaagatt gcgttaaatg attttagtgc agaatcattg tcggcatttc gtttaatttt
     6841 agcaacaata attctcttgc cgttcgtaat tataaagaaa ttgcctaccc ctgaactaag
     6901 agatatccct gttattttta ttttaggatt ttgtgaattt gtgatatatc acacagcttt
     6961 aaattttggt gaaactttga ttagtgcagg tatatctgga attctagtct ctacaacgcc
     7021 tattttttct agtgctttag cttatatatt tttaaaagag catttttcaa aatggaattg
     7081 gttaagttca ctcgtcgcat ttataggaat atccattatt tcaataagta aagatgatta
     7141 cacaactatt aatgtattag gtgttcttat tattttactt gcatctttta gtgaaagttt
     7201 gtattttact ttccagaaaa aatacataga aaaatacggc ttcatcgctt tcacactata
     7261 tacaataatg gcaagctcac catttatgct tatttttatt cctgaaatca tcaacgatat
     7321 acacggcgcc acttttacat caatagtatc ggtactttat ttagctatat tccctactat
     7381 aattccatac gttttgcttg cttatattgt gaagtcagtt ggtgtctctg atgcaacaat
     7441 gtctctttat ttaacaccta tcgtttcttt attattatct tatctgttat tagacgagct
     7501 accaacaacc cttgctatta taggcggaat tatcactcta ctaggcgtta gtttaagtaa
     7561 cttctttcaa aatacataat tattccaagt cccgcacctc agaatccaaa aacattcgag
     7621 tgataaaatt ttaaaaatca aaaatataaa aatgatctaa tttcgtcaaa tttaccaata
     7681 taaatactaa tatttgcaat tcacaaaggg gtatagtctg agtgtattct aatacgaaag
     7741 gacttggtgg atatgtatta cagttatgga aattatgaag catttgcgcg ccctaaaaaa
     7801 cctgaaaatg tagaaaacaa atccgcttac ttaatcggat ctggtctagc ttcacttgct
     7861 gcagcttgtt ttttaataag ggatggtcaa atggaaggtt cgaagattca tattttagaa
     7921 gagttaccta aagcaggtgg tagtcttgat ggtgaaaata tgcctttaaa aggctatgtt
     7981 gtccgcggtg gtcgtgaaat ggagaaccac tttgaatgtt tgtgggactt attcagatct
     8041 atcccttcat tagaaatcga taacgcgtct gtattagatg agttctattg gctaaacaaa
     8101 gaagacccta actattctcg ctgtcgtgtt attgagaaac agggtcaacg tttagtcaca
     8161 gacggagact tcactttgac taaaacggcg attaaagaaa ttttagattt atgcttaacg
     8221 aatgaagaag atttagatga tgtcaaaata acagatgtat tttccgatga cttctttaat
     8281 tcaaactttt ggatttactg gaaaacgatg tttgcatttg aaccgtggca ttctgctatg
     8341 gaaatgcgtc gttatctaat gcgattcgtt catcatattg gtggtctcgc agacttttca
     8401 gctttaaaat tcactaaata taatcaatat gaatctttag tattacctat ggttgaatat
     8461 ttaaaatcgc atggggttca atttgaatac gatgtaaaag tagaagatat taaagtagat
     8521 gttacgacaa gtcaaaaaat tgcccgagaa atattaattg accgtaatgg taatgcagaa
     8581 tctattaaac tgactgtaga tgatcttgtc tttgtgacaa acggtagtat tacggaaagc
     8641 tctacgtatg gtgataatga tacaccagca ccaccaactg acgaattagg tggtagttgg
     8701 acactatgga aaaatttagc gcgacaaagt cctgaatttg gtaatcctga taagttttgc
     8761 caaaatattc ctaaaaaaag ttggtttgtt tcagcaacat cgacaacaaa caataaagat
     8821 attatcgata caatagaaag catttgtaaa cgtgatccac ttgcaggcaa aacggttact
     8881 ggcggtatta ttacaatcaa tgattctgca tggcaaatca gttttacaat caatcgtcag
     8941 caacagttta aagaccaacc tgaaaatgaa atatctacat ggatttatgc cttatattca
     9001 gatgtaaacg gcgattatat taaaaagcca attacagaat gtagtggtaa tgaaatatgc
     9061 caagaatggc tgtatcactt aggtgtatca actgacaaaa ttgaagactt agcaaaacat
     9121 gcatctaata cgattcctgt ttatatgcca tatatcacct cttatttcat gacgcgtgct
     9181 atcggcgaca gacctttagt cgtcccgcat caatcacaaa atttagcatt tattggtaac
     9241 tttgcagaaa cagagcgaga tactgtattt acaacagaat attcggttcg tactgccatg
     9301 gaagctgttt atcaattact aaatatagat cgtggtattc cagaggtcat caatagtaca
     9361 tttgatcttc gcgtcttaat ggatgccgta tacgaactga atgaccatca agatttgcgc
     9421 gagattacta aagattcgaa aatgcagaaa ctcgcattag caggattcct taaaaagata
     9481 aaaggtacgt acattgagtc attattaaaa gaacacaaat tgttataacg aaaaccatta
     9541 atagattttt atttggtgat ttcaaatcat ggagtgggat agaaatgata tgttcacaaa
     9601 ctttatttcg ttatcccact cacatgattt ttttgatgaa acataattac atgattgatt
     9661 gcgtcatttt gttaaacaag tgattgcaaa cctgccattt cacactgaaa atttacataa
     9721 taagtgacga tattttacaa gtcatataca aataacatat attgttaaat aattttacct
     9781 aatcttaaca ttaaatttac aattataagc gataatctaa atataaagct tatttgaggt
     9841 gaaataatgg aaatgtcggt tacagaagtc attttctcct ttttaggtgg tttaggtatt
     9901 ttcctatacg gcttaaaaat catgggagac gggcttcaag catcagcagg agacaggcta
     9961 cgagatattt taaacaaatt tacatcaaat ccagtattag gtgttattgc aggtatcgtt
    10021 gtaactattt taattcaaag tagttcaggt acgacagtta tcacaattgg acttgtcaca
    10081 gctggattta tgacattgaa acaagccatt ggagtgataa tgggtgctaa tatcggaaca
    10141 acggtaactg catttattat cggtatagat ttaggcgaat atgcaatgcc aattttagca
    10201 ttaggtgcat ttttaatctt tttctttaag cgctctaaaa tcaataacat tggtcgcata
    10261 ctattcggtt tcggttctct attcttcggt ctagaattta tgggtgatgc cgttaaacct
    10321 ttagcatcat tagatggatt taagcaatta atgcttgata tgtcatcaaa tccaatactt
    10381 gctgtcattg tcggcgcagg gttaacagca ctagttcaaa gttcaagtgc gacgattggt
    10441 attttacaag aattttatca acaagattta atcagcttaa acgcagcaat ccctgtgtta
    10501 ctaggcgata acattggtac aacgattaca gctatcttag ctagtttagc cggctcaatc
    10561 gctgcaaaac gtgcggcgct tgtacacgtc atctttaact taatcggggt aattatcttc
    10621 acaattttct tgccagttgt gattcatttg attagtttgt tacaagatgt atggcactta
    10681 aaaccagcga tgacgattgc agtatcacat ggtatcttca acataacaaa tactttgatt
    10741 caattaccat ttgtagcagg tttagcatgg attgttacaa agcttgtccc aggtaaagat
    10801 attgctgatg actataaacc tcagcactta aacaaagatc ttgtttatca cgcacctggt
    10861 gttgcattac aagaaactca aaaagaatta caaaatgttg gtcagattgt cttatcaatg
    10921 tttgaagaca ttcgcgaaat tacaaaagac gataaaaaat tgatcaaaaa gcttgaacaa
    10981 aagcaccaag ctgttgaaac aatcaatgat agcattcgaa attatttagt tagaatttct
    11041 acaaaagcca ttacgaaggc agacgttgag cgtttagcag ttatgtttga tgtcaatcgc
    11101 tctattttaa aagtagcaga gctaacagaa gagtatgtcg ctcaattaaa acgccaacat
    11161 gatgaagata ttcgcattac agaagatgca caacgcggta tggataaatt attcaaccat
    11221 gttgctgagt catttgataa agccatcgac atgttagatg tttatgacaa aacgaaaata
    11281 gatgaaattg tagaacgtag tagagaatca tttaatattg aacataaact acgcaaaggt
    11341 catattaaac gccttaatcg tggtgaatgt acaacaaaag gcggattact atatatcgat
    11401 atgattggtg ttcttgaacg tatcggttat cattcacgaa atgtttctga agcacttgtt
    11461 ggccttaacg atgatgtacc tacagatgaa gaaattgcaa caactgaaat ttaattttta
    11521 ctgtcttatt taaattcata tcttatataa aattagagat tcagatgcac gtaaaaggcc
    11581 aatccaacat cactgggttg gcttttttgt ttagcaaact ttattatctt aaatcggcta
    11641 taaacactga tataatgatg cttcattagt atgcggtaag catgacggac actgttctcg
    11701 gagtctgacc tcgaatcatt taatatacac ttttacacgt cgccttcatt gaagtgaaat
    11761 gccataatct tcacattaga tatagttcct tccatataaa tatccaaatt ttagaacaac
    11821 gcaataaata accatccacc taacttatca aaagtttaag tggatggttt ttcatttata
    11881 tttatattag tgttaatcca atcatagatt tatctatatg cactgctcta tacatttcct
    11941 catttaattt gctttacttt catttatatc attatcaaaa cacttggcgt gtcatcgtta
    12001 ttatttcgca tctttgacac gtttatcatc attaggaatc gcgaataaaa ttgcgataaa
    12061 tgccatgatt cccattaata cgttaaccca aagtgcaatc atcgcacctg tatgaatgct
    12121 cgttgcagca actgcaccgg catatacagc accactaatt gcgacaccga atgcgccacc
    12181 aagtgatgaa gccattttat aaatacctga agcaacgcca actttatcta acggtgcatt
    12241 cgaaatagct gtatctgtag aaggtgttgc ataaatacct aagcctagtc cgaaacataa
    12301 atatcctacg acacaactga taacataaaa tatgcctggt aagaatacta atgaaataag
    12361 tgcaatacca atgaccacaa tgaatgtacc taataacatt ggtcgcttag aacccatttt
    12421 ttgtaataat ttttcaccaa ctcgaatcat caataacacc atgattaaat aagtaattga
    12481 taagtatcct gcctgcaatg ctgtataacc taaaccttgt tgcacgaatg tattcgctac
    12541 aattaatgta cctgcaacac cgtttaataa gaagttcgaa atcgttgcac ctgtatatgg
    12601 tttattttca aataatttaa aatcaataag cggattatct acttttttct caacttttaa
    12661 gaaaataaag aatgctacaa ttacgattgc aattaaacca aagaaccata atgatgtata
    12721 accaagtgct gcacctttag taatgacaac gtttaaactt agcaacataa ctactagaac
    12781 aattagccct gcaacgtcaa atttatgtgt attgataact tctgatttcg tttcaggcgt
    12841 ccctttgatg agtaacattg aaaatacggc aacgataatt gagaagatga aaatccatct
    12901 ccaacccata gttgtcgcaa ctgccccacc gaagagtgaa cagataccac ttccacccca
    12961 agaaccgata gaccagtagc ttaaggcgcg ctgacgttca gcaccctcat aataagtttt
    13021 cataatggcc aatgtggaag gcataataca cgctgctgat acgccttgta taatacgacc
    13081 taaaattaat aatgccggta aattcgtaat aataattaat gctgaaccaa taatacttaa
    13141 caataaaccg atattcgtca ttttcacgcg cccaatttta tctgctaggc cgcctgctcc
    13201 aacgacaaac atacctgaaa atagtgcagt tagactgacc gcaatactaa ttgtccccat
    13261 gtctgtacca aaactttgtt gtaaattcgg tacaacattt acaagtgatt gtgcaaacaa
    13321 ccaaaatgta ataacaccta atacaatacc taagattaac ttgttgcccc cgcgatacgt
    13381 ttcattcatg ttagttatct cctttaaggt aatctaaaac aactgtccct acagcttctg
    13441 cagaaataag taatgatttt tctgaaatgt taaatttagg atgatgatgt gggtaaattt
    13501 caccattttc caccgctgca cctgtataaa taaaggcact tggacgttct ttagcatagt
    13561 atgcaaagtc ttctgaaggt ggttgtggtt cacatatttc gacaccaaat tcaaggtctg
    13621 cttcttttaa cgtcttagcc acgtactcag taaactctgg atcattatat aatgcaggat
    13681 aatcatcgtt atattctaat gtgcaagtta caccattcaa tgcttctaat ccttttgata
    13741 aacgtttaat ttctgtttca attgttgctt ttgtagcatc tgttaatcca cgtacatcac
    13801 cttcaatttc aacaacatct ttaatgacat tgaattgacc tttaccgtca aatgaaccga
    13861 ttgtgacaac accggtttca aatggactta gtcgtctaga tacaactgtt tgtaacgctg
    13921 tgacgaagta gctacctgca acaatggcat cattggccat atgtggtgat gaaccatgac
    13981 cacctttacc ttgaactttc aatttgaaga atgcgcgtcc tgtttgaaca taaccaggtc
    14041 tgtaatacac attacctgtt ttcattgtgc tcatgacgtg tacacctaat acatgatcaa
    14101 caccgtctaa tacaccattt tcaatcattg ctttagcacc acctggtggt acttcttcag
    14161 ctggttgatg tatcacaacg acttttcctg taaaactatc tttcatttca gcaagcgtct
    14221 ctgctaatac aagcatgtat gctgtatgtg catcgtgacc acatgcgtgc ataacacctt
    14281 tattttgtga tgcaaaagat aatcctgtat cttcagtaat gggtaatgcg tcaaagtctg
    14341 cacggattgc taatgtttta ccaggtttcc ctgaatcaat cgttacttta attccacgtg
    14401 gtccgacatt cgtttctact tccacatctt tacctttgta aaattcagcg atgtatttcg
    14461 ccgtttcatc ttcatgaaaa gataattctg gatgctgatg taaataacgt ctgatctcaa
    14521 tcattttgtc ttctttagat tttaaagctt caattaattg ttgattcata tccttcatct
    14581 ccttagttac atcataaatg attaatcatt atttatattg ccaacaacag agatgttaac
    14641 cattaatttt ttgcaatttt agctttgaat ataaaaaatc acaaattatg tatatcaaaa
    14701 tttgtgattt gtgatcattt tatgaacttg ggtaacgttt tacttcaatt aagtgaatcc
    14761 cattcgtaat cattttaatg tttaatgcca gtgtgtccgt gatatctata tcatatactt
    14821 ctaatttcgg aaaactcatt cgattaacgt aatctataga gtccttgtcc atgccatgta
    14881 tcgtatgatg tttgcgccaa agattaaata acgcaccatt ttctttatct aaggtaaaat
    14941 gtttaatctt atacataccg tcttctaggt cattaatgtt caaatgaatc atttccgtcg
    15001 cacgcatatt catttgattg tccagcgcta agtacggatt aaaatgcttt gcatcatata
    15061 acaatatttg aaaatttgaa tcagttcccg tgacaataca tgtatcatca gaatacaaaa
    15121 tattgcttgt taatttatta aatagcaatg ccgtgaaata gaccggacgt tttccattat
    15181 attgatgaaa cagttcaata gaattcatat aatcccgttc atttctacaa tgactgacgt
    15241 gcaaatcata attcaaccaa tacccgatac cctctacttt agtacttaat tttaataatt
    15301 gctcaatgat gataccacct ctaaaatatt cgccgtttgt aataaatgta tcacccgtca
    15361 atgtattcca attgagtaaa atgagtggac gctttaggtt atggcgatgc attaagtcga
    15421 taaggtaatt cgttttatta ataatcattt gacttgcggt tttaaattca tcatcattca
    15481 ttttattaaa atcaacagcg tcatttgaat tggcatcaaa tacaaaatgg tcgatgtgtg
    15541 gctcaagtcg tttcaacaat ggtagatgtc tttgggttgc ttgatctaag tgaatgtaca
    15601 agccaccatt agggaataac gctttaaaat aatcaatcat ttcaatcaaa gaagtgtgca
    15661 atgtcgtcac atacaagttg aacttcaaat cttttctatg actgacatgc agggcaacgt
    15721 gatggataaa tactttgaat gcatcgatat aatcacgtga gtcatactga tccaaatgca
    15781 ttgtcaaact aaagttatga tctaataaaa agtctaaaca caaatcgata tcataaaata
    15841 tattcgaaat ttctgcatca tacgtgaatg gcgcattgag ctttttcata atatatggca
    15901 tcacatcata tgctaatact tcattgacat gaaaatcatg atggcatgta agtaactgtg
    15961 attgatactg tgtattgagc aaattcctca aatagcctac ttgaataata tgattaaatt
    16021 gatttagttg ttgattattt ggtttaaagg caatctcttt atagttcatc ttttcaatat
    16081 cttcaataaa atgattcatt tcttcaatgt agtcatttaa aagtaatatc aattcacggt
    16141 cgtgataatc atgttgtgcc gattgctggt tttcagtgat tgctggacga tcacctcgat
    16201 attgtttagg tgtttgatgc gtaaagtgtt taaatgttct cgcaaagctc gctgcacttg
    16261 aaaagccatt ttgcaatgca atatcagtaa tggtttcatc tgtgtatgtc aaatcgagtt
    16321 tcgcatgctc aattcgcgtc gtatttaaga aatgctggaa tcctacacct agcgattctg
    16381 taaacttttt agacagatgg ctctctgacc acccaacata ttcgcttaat tccgaaaggc
    16441 ttaagtcttc atgaaaatgt aactcgatat agtcgcatac ttgattcact ttatcatcat
    16501 ttaagatact ttggttcgaa tgatatgtac gcgggacata atgaatcata tgcataagca
    16561 actgaatgac aagttgttgc tcagtcaatt tagacaactc attatgtcgg atatgtgttg
    16621 aaaccagtct tgccattata tttctcagtt gatgtatatt ctttgttgtg gtcgcatctg
    16681 ttaagtgaaa atatagacaa tgcgcatcat caaacttgtc tgctaaatat ttcatttgga
    16741 attggatata acatatgatg ccatcttgtt gaagttgaaa tcgatacaag tcgcggtggt
    16801 taatgatgaa aatgtcgcca ctgttgcatt gcgtcatatt attttcatca taaatgtgtg
    16861 cctcaccttt aataacaaaa ccaatcatta aactattgag ccttttgaaa tctgacatac
    16921 tctcagtttc tactcgaatt aaataatcac gttgcatact atccctcaat tcagtaatat
    16981 gaatacgttt attttacatt attttacagc aacatatttg aatttcatat tgaatcgtgt
    17041 gtgtggatga ttatttatcc tcactcggtt caagatgtag actatcagta aaaaaagtat
    17101 tttcaccttt tttctccaca aaagtaaatt caatgtcttt atatccaact gatgaacctt
    17161 ttaagtctcc cgaacctttc aacaataact ttggtgcttt attcgttggt attttatatc
    17221 tttttcgtaa ttgttttaca ttatagtcat cattagttaa ttgatatttt gctgaataac
    17281 tcggcacctc tggattgtat gatatatcgc ggtctttgta cttcgacaaa tctttaaagt
    17341 cgccatattg aacaaagaac ttaaagtttt cgatttcttt ttttattttt tcatctttaa
    17401 tttctttagt tggaatgatt ttattatcga ccattttaac cggatatctt ttttcgttat
    17461 cacgatgtat acctttatct tctctttcta taacatcaac atagtaatag ccatttgttg
    17521 ttcttgtatt tctattcata tataaaacca tgccttttat attcatatct tttccattag
    17581 attgaatcga cattgacgaa cgaacaatcc atgttccctt atcattttta tcaaattgat
    17641 catcacgata tccttcttta tcgtataaat cctctagatt tttaatcgga tacatactca
    17701 acgttttttc aaaacttttc tttatttccg cttctttccc tgtaccacaa ccagcaaaga
    17761 tactgatgat taaaaacaga aaactaatac ctagcactaa tttatttaca cgttttatca
    17821 tttcatcact ctctttttcc gggttcgatt tcatactctt tattggtcac gtttatttat
    17881 cctcacttgg ttcaagatgt agactatcac taaagtatat attttcacct tttttctcca
    17941 cgaaagtaaa ttcaatatct ttatatccaa ctgatgaacc ttttaagtct cccgaacctt
    18001 tcaacaatag ctttggcgat ttatttgttg gtatatcata tcttttacgt aattgtttta
    18061 cattataatc atcattcgtt aattgatatt tagctgaata actcggtacc tctggattgt
    18121 atgaaatatc tccatctttg tacttcgaca aatctttaaa gctgccatat tgcgcgaaga
    18181 acttaaaatt ttcgatttct ttttttatgt tttcgtcttt gataccttta gttggaatga
    18241 ttttattgtc taccattttc accggatatt ctattttatt atcctgaggt ctaccatctt
    18301 catctttctt tactgaatta acatagtaaa atccttttgc acttcttgta tttctattca
    18361 tatataagac cataccttta actttcagag cttccccctt attttgagtt gccatttcag
    18421 aaccaataat ccatgtacct ttatcatttt tatcaaattc gtcatcacga taaccttctt
    18481 tatcgtataa atcctctaga tttttaatcg gatacatact caatgttttt tcaaagcttt
    18541 tctttatttc cgcttcttta cccatgccac aaccagcagt gatactaatg actaaaaaca
    18601 gaagactaat accaagcact aatttattta cacgttttat catttcatca ctctcttttt
    18661 ccgggattga tttcatattc tgattgagcc atgattatac atctccgctt tttttataat
    18721 ctaagctatc actaaagtat atattttctt cttttttctc tacaaacgta aattcaatat
    18781 ctttatatcc aactgatgag ccttttaaat tccctgaacc ttttaacaat aactttggag
    18841 ctttactcgt cggtatatca tatcttttgc gtaattgttt tacattataa tcatcattag
    18901 ttaattgata tttagccgaa taactcggca cctctggatt atatgaaata tctccgtctt
    18961 tataattttt caaattttta aagtcgccat attgaacaaa gaacttaaag ttttcgattt
    19021 ctttttttat tttttcatct ttaatttctt ttgttggaat gattttatta tcgaccattt
    19081 taaccggata tcttttttca ttgtcgtgcg gttttccttc atcctcgtcc ttagtcacat
    19141 cgacatagta gtaaccattt gttgttttgg tatttctatt catatataga accatgcctt
    19201 tagctaccat atcttcatta ttaggttgaa taaccatttc agaatttata atccatgtac
    19261 ctttatcatt tttatcaaac tgatcatcac gatagccttc cttatcgtat aaatcctcta
    19321 gatttttaat agggtacata ctcaatgttt tttcaaagct tttcttaact tccgcttctt
    19381 tacctatgcc acaaccagca gtgatactaa tgactaaaaa cagaaaaata atgcctaaca
    19441 ctaatttgtt taatcgtttc atcatcttac aactctcttc tttttaaaat gatttgtttt
    19501 tgttaattac atatatttat caatttataa cactttttca ctttaatatt atcaaaaatt
    19561 tcacaaacaa tctgttcaat ttataatatt gttgaattat tacttatttt caaaataaaa
    19621 acgagtagtg aaaataatta gaatctaact attttcatac tcgtcttaat atcattattg
    19681 aatccttatt tattactatc tataatttca cttacgatat catcatatcc aggccacgta
    19741 tatcctgcat agtcaacaat cagccaaccc gttctagctt tcccatttgc tttaatcgtt
    19801 tttgcaattt caggatttat atgtgatgca tagttataag tactattaaa tgcgctgcct
    19861 ccagacgcta cactcaagaa attcacatat acattgtctt tgttactatc cgttttagct
    19921 ttagccaata aatttttaac agcttcaact tttttatcat agtaatcttt atactcatct
    19981 tgtactttta aatttaagct accattatta attttcgttt caaatgtcgc attgtctgcc
    20041 cattgaatac ctgacgtgtc agcaccataa ccacttttta tgtacgtacc ccccattcta
    20101 ttgaatagga caattttacc tttcgtttct tttaaagtag gattcgcatt acttcctgtg
    20161 taaaaaagat tctgatattg cgggttatta taataatatt ctctaaaaat ttcttcaaat
    20221 gtcttcgtaa ctttagaatc gctatcgtag tcctttttca tagacatcac aattgtttcg
    20281 tttggataag cactcaagta atatttagca tcatcgagaa attttcctaa ttcatgatgc
    20341 aaataaacca tgccgtgatg aactgaaatc atattatcag cacttgctct acctctaata
    20401 tcaaaaaacc tgactcccga cttcatttgg gtaaggtaat ctttatcttg agtctttgcc
    20461 caaactgatt ttactggatc ctttaaagtg aatgagccac tatcatgtga acccggtata
    20521 ttaatctcag ttaaatgttt tccatcatca agtttactca tccaattttc tggactttta
    20581 ctcaacgaat ctgacgcatg tgctgaatga taccaaccac tcatcactac taaaatgata
    20641 cttaaaaaca aagtcttaat acactttttc ataatatcaa cttctttcta tatttaatac
    20701 attaattata catctttttt aaataaaaat atgtgtaaaa ttttaaaact t
//
LOCUS       DAHHYJ010000031        20569 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000031 DAHHYJ010000000
VERSION     DAHHYJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20569
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.031"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            42..1499
                     /locus_tag="NBK22_000881"
     CDS             42..1499
                     /locus_tag="NBK22_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD2414084.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGINAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLIVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1492..2214
                     /locus_tag="NBK22_000882"
     CDS             1492..2214
                     /locus_tag="NBK22_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD2414085.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2365..3492
                     /gene="queG"
                     /locus_tag="NBK22_000883"
     CDS             2365..3492
                     /gene="queG"
                     /locus_tag="NBK22_000883"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCD2414086.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3497..3967
                     /gene="trmL"
                     /locus_tag="NBK22_000884"
     CDS             3497..3967
                     /gene="trmL"
                     /locus_tag="NBK22_000884"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCD2414087.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEVTNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4126..4725
                     /locus_tag="NBK22_000885"
     CDS             4126..4725
                     /locus_tag="NBK22_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCD2414088.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYHENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4750..4902
                     /locus_tag="NBK22_000886"
     CDS             4750..4902
                     /locus_tag="NBK22_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414089.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            5679..6074
                     /locus_tag="NBK22_000887"
     CDS             5679..6074
                     /locus_tag="NBK22_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414090.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            6270..7655
                     /gene="fumC"
                     /locus_tag="NBK22_000888"
     CDS             6270..7655
                     /gene="fumC"
                     /locus_tag="NBK22_000888"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCD2414091.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFSLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQIDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(8108..8929)
                     /locus_tag="NBK22_000889"
     CDS             complement(8108..8929)
                     /locus_tag="NBK22_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD2414092.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTIDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTIDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKEAVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            9091..10203
                     /locus_tag="NBK22_000890"
     CDS             9091..10203
                     /locus_tag="NBK22_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCD2414093.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQRRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGMSSESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            10225..10848
                     /locus_tag="NBK22_000891"
     CDS             10225..10848
                     /locus_tag="NBK22_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2414094.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
     gene            11204..11668
                     /locus_tag="NBK22_000892"
     CDS             11204..11668
                     /locus_tag="NBK22_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2414095.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            11847..12971
                     /locus_tag="NBK22_000893"
     CDS             11847..12971
                     /locus_tag="NBK22_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCD2414096.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQIDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDASQFNEIAENLSVYITTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEKLSIHLSRIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            13040..13384
                     /locus_tag="NBK22_000894"
     CDS             13040..13384
                     /locus_tag="NBK22_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCD2414097.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            13878..13976
                     /locus_tag="NBK22_000895"
     CDS             13878..13976
                     /locus_tag="NBK22_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414098.1"
                     /translation="MPFDSLAVLITTEIAFPTEIFEKYQRTLQIKF"
     gene            14267..15463
                     /locus_tag="NBK22_000896"
     CDS             14267..15463
                     /locus_tag="NBK22_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD2414099.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQAVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            15453..18389
                     /locus_tag="NBK22_000897"
     CDS             15453..18389
                     /locus_tag="NBK22_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2414100.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEENKIEDNAVHSELDSVEEKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            18386..19327
                     /gene="yhaM"
                     /locus_tag="NBK22_000898"
     CDS             18386..19327
                     /gene="yhaM"
                     /locus_tag="NBK22_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCD2414101.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYAYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(19448..20410)
                     /locus_tag="NBK22_000899"
     CDS             complement(19448..20410)
                     /locus_tag="NBK22_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCD2414102.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSEVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         7542 a         2892 c         3639 g         6496 t
ORIGIN      
        1 atatgtataa ttatgaatat taattaggga gggctgtata aatgaagtgt ttaattaggt
       61 ttatattagt attagggttg ttaattagca gtgctatggt gtatatcaat cccacagcac
      121 atgctgaaca agatcaaaca tgggagaaga ttaaagaacg cggtgaactt agagtgggtc
      181 tttctgcaga ttatgcacca atggaatttg agcatacagt taatggtaag actgagtatg
      241 caggtgtaga tattgattta gctaaaaaaa ttgcgaaaga taataattta aaattaaaaa
      301 tcgtcaatat gtcatttgat agtttgttag gagctcttaa aactggaaaa attgatatta
      361 ttatttccgg aatgacttca acgcctgaac gtaagaagca agttgatttt tcagattcat
      421 atatgatgac taaaaatatc atgcttgtaa agaaagataa agttaatgaa tataaagata
      481 tcaaagactt taataataaa aaagtagggg cacaaaaggg aactgaacaa gaaaaaatcg
      541 ctcaaaccga aattgaaaat gcatctatta cttcattaag ccgtttacca gacgttatat
      601 tagcacttaa aagtggaaag gttgaaggtg ctgtagttga aaaacctgta gcagaagcat
      661 atttaaaaca aaatcctaaa ttaggaattt ccaatgtgaa atttaatgaa gaagaaaaag
      721 atacagtgat agcagtgcca aaagattcac caaaattatt gtcacaaatt aataaaacga
      781 ttaaagaggt taaagataaa ggattaatcg ataaatatat gactaatgct gcaaatgcga
      841 tgaatgatga cagtggtttt atttctaagt atggtagttt tttcttgaaa ggaattaaga
      901 ttacaatatt aatttcactt atcggtgttg cattaggttc tattttaggt gcattcgttg
      961 cgttaatgaa attaagtaaa ataaaaatta tttcatggat tgcttctatc tatatcgaaa
     1021 tattaagagg aacaccaatg ttagttcaag tatttatcgt attctttggt ataactgctg
     1081 cattaggact agacatttca gctcttgttt gtggaacaat tgcattagtt attaattcct
     1141 cagcttacat tgctgaaatt attcgtgcag gtataaatgc tgttgataaa ggccaaatgg
     1201 aagccgcacg tagtctaggt ttgaattata gacaaacgat gaaaagtgta attatgccac
     1261 aagcaattaa aaatatttta ccagctttag gtaatgaatt tgtcacttta attaaagaat
     1321 catctattgt gtcaacaatt ggagttggcg aaattatgtt taatgcacaa gtggttcaag
     1381 gtatttcatt tgacccattc acaccattaa tagtggcagc agcattatac tttgtcttaa
     1441 catttgtact tacacgcatt atgaacatga ttgaagggag attgaatgcc agtgattaaa
     1501 ataaacaatc ttaataaagt ttttggagat aatgaagttt taaaagatat caatcttgaa
     1561 atcaatcaag gggaagtagt agcaataata ggtccatctg gtagtggtaa aagtacattg
     1621 ttaagatgta tgaatttatt agaagtaccc actaaaggtc aagtgatttt tgaaggcaat
     1681 gacttaacgg aaaaagggac acaagtagat aaactacgtc aaaaaatggg tatggtattt
     1741 caaaacttca acctatttcc acataaaaaa gttgtcgata atattatttt agctcctaaa
     1801 ttattaaaga aagataataa cgatgaatta cataaggaag cattgtcgtt attagataaa
     1861 gtgggattaa aagaaaaagc agatgtatat ccgaatcaat tatcaggtgg tcaaaagcaa
     1921 agggtagcaa ttgcaagagc tttagcaatg catccagatg ttattttatt cgatgaacca
     1981 acttcagcat tagatcctga ggtagttggt gatgtattaa aagtaatgaa agacctagcc
     2041 aaagaaggta tgaccatggt ggttgtgaca catgaaatgg gatttgccaa agatgtaagt
     2101 gacaaagtca tatttatggc agatggcgtt gtcgtagagt caggcacacc agtcgaaata
     2161 tttgaacaac cgcaacatga aagaacacaa aatttcttag caagagtatt ataacaacct
     2221 aacgaagctt gaatatatga tacgcaccac aaagttatat catatattca agctttttat
     2281 tattaaaatt atgtgaaatg aaacctcaat atgaataatg gttgtgttaa aataacatag
     2341 tattatttta tggggggcat acgattggat acaaagcagt taaagcaaga tatcattgac
     2401 tatgcatata caattggtat cgacagtatt ggatttacta ctgccgatcc ctttgatgaa
     2461 ttgaagcaaa agctagaagc atatcatgca aatggttatg cctcaggatt tgaagaatct
     2521 gatattgctt tacgaacgga gcctaaatta tccttaccaa cagcaaggtc aatcatagca
     2581 attgcagttg gttatcctaa caaactgaaa ggtgcaccta agagtgttag aggtgatcgc
     2641 agaggcttat ttgctagagc atcgtggggt caagattatc atacaattat gcgtaaacga
     2701 ttagacatgt tagctgcatt tattgaatct aaagttccag atgttgaaat aaaatctatg
     2761 gtagatacgg gtgtattatc agatagggca gtagcagaac gtgcaggttt aggatttgtt
     2821 ggtcgtaatg gttttgtcat caatcctaaa ctaggaacat ggacatacct cggtgaaatg
     2881 ttagtcagta taccttttga acctgatgat ccattattag atagctgtgg tgattgtaca
     2941 atttgtgttg atcgttgtcc aacaagtgca ttagttggaa atggccaatt aaatagtcag
     3001 aaatgcatta gttttttaac gcaaacaaaa ggctatatgc ctgatcaata tcgttataaa
     3061 attgggaata gattatatgg ttgcgatacg tgtcaacaag tttgcccgaa aaatagaggt
     3121 attaataccg aacaagatga catcattttg gaaccagaaa ttttaaagcc aagattagta
     3181 cctttactac gtatgtctaa taaagaattc aaacaaacat atggtcacct tgcaggtgct
     3241 tggcgtggta aaaagccgat acaacgaaat gctattttag cattggcgca ttttaatgaa
     3301 gtggatgcaa ttccagaatt gaaaaaagtt gcaacaacag atgaaagacc gttgattcga
     3361 gcaactgctt attgggccat tggtcaaatt cttggggaag aagcaagaga ttttatcaat
     3421 gctaattatg atcaagagga tgcagaagtt cagaatgaaa tgataaaagg attagacaca
     3481 aggagagaat agtaatatga caaatcatat cgttttatat caaccagaaa tcccagctaa
     3541 tactggtaac attgcacgta cgtgtgcagg tacaaacaca catttacatt taattaaacc
     3601 tcttggattt agaactgatg ataaaatgtt aaaaagagcc ggtttagatt attgggaatt
     3661 tgtgaatatt acgtatcatg atagtattga agcatttttt gaagtcacaa atggcgagta
     3721 ttacttatta acaaaatttg gtaaaaagac ttatagtgat tttgattttt caaatcatga
     3781 caaagatttt tacttcattt ttggaaaaga aacgacagga ttaccagact gggtgaaaga
     3841 gaaatatcaa gacacagcgt taagaattcc tatgagtgaa catattcgtt cattaaattt
     3901 atcaaatact gctgcgttgt taatttatga agcgttacgt caacaagatt ttccgggatt
     3961 gaattaaaat ttactcattt tcattataaa aatgatgctt aagtgctata tttagagtat
     4021 aagcatgtta aagttaaatg gtttgatata ttgaaagtta gggtataata attattaata
     4081 tcgccgtttt tatgtaaaat gcaaactaag aggagtgtag aaatcatggc aatgaacttt
     4141 aaagtctttg acaatagtca acttgtagca gaatatgctg ctgatattat tagaaagcaa
     4201 tttaacaata atcctactac aattgcaggt tttcatttag atacagatca agcgccagtt
     4261 ctagatgaat taaagaaaaa tgttgaaaaa catgctgttg attttagcca aataaatatt
     4321 ttagattatg acgataaaaa atcatatttc gaagcgttag gtgtaccagc aggtcaagtt
     4381 tatccaattg cttatgaaaa agatgcaatc gaattaatcg ctgataagat taaaactaaa
     4441 gaaaataaag ggaaattaac attacaagtt gtttctatcg atgagcaagg taagttaaat
     4501 gttagtattc gtcaaggact aatggaagca agagaaattt tcttagtagt gacaggtgct
     4561 aataaacgag atgtagttga aaaattatat catgaaaatg gtaaaacaag cttcgaacca
     4621 gccgatttaa aagcacatag aatggtaaat gttattcttg ataaagaagc ggctgcaggt
     4681 ttacctgaag atgttaaagc ttactttacg tcacgctttg cttaattaaa aatctgtgag
     4741 gtattgatta tgtctaaaga caaagatcca aaattaaatt atcatgaaga agaaaacagt
     4801 atggtaacgg attttgaaga tttaaaagaa ttaggtaaag aaatggaaca aatttctgat
     4861 caaaatgatc aagaaaagaa ttctgaagaa gacagtcagt aaagtattat aattgtgtaa
     4921 tatacagcta tttatcatgt aaataatgta tagtatagaa catatatcaa ataggattga
     4981 ttatcttgaa tatgaagtta ggtagcattt tgttacctag cttttttatt atattcatag
     5041 aagctgactt tctgccagtt tcagtgttgg ggctgtttcc ccaacttaca ttgtttgtag
     5101 aaattgagga tccaattttt ctatgttgtg gccccgccaa cttgcatgtt attttaagct
     5161 gactttccac caacttctga gtttgtggcc cgtttcccta atttgcaatg tctgtagaaa
     5221 ttagaaatcc gatctcacta ttttggggcc tcgccaactt gcatgttatt gtaagctgac
     5281 tttccaccag tttctgagtt tgtggcccgt ttccccaact tacattgttt gtagaaattg
     5341 aggatctaat tccactttgt tgtggccccg ccaacttgca tgaaatagaa gctgacattc
     5401 cgaagcctct atgttgtgga ctgcagttaa ctactgccaa tataactttg taaagcataa
     5461 aacattgatt tgtgtaccag acatagtaat atatcaaaag ttcaatttta aatattaaaa
     5521 caccatttaa acggaattga taattaatta ctccagacat aattgaaaga agtcatctat
     5581 cagtaatatt tttaatgaaa tcattaaacc gcgtttctta atatgccaag attatgatat
     5641 gctattgaag aatgaaatta gaggagattt atatatttat gttaggaaaa tactggattc
     5701 aattattgat agcaacagtt attataagtt taatatcaat taaagctttt ccattggcaa
     5761 ttggggcatt atatttacct gttattttca aagtaataaa attacaactc aatttatcaa
     5821 aaggattaat cgatgatgtt aatgcgcaaa cattcattaa aagtaatcaa tcaggtattg
     5881 taataagtgt aatttgttgt ttattaataa caggaatatt gtattataca cttgatgggt
     5941 tttatgcgag tttaacagga gtattaggta cgcttgttgc tctaaatcca tatacgacaa
     6001 ttgttagtgc tgtattatat atacttacag ctattgctac ggttgaagct actaaaacaa
     6061 agtatcgtaa ttaaacttag ttcaacaata actaaataat gatagataac acactaatcg
     6121 tatgaccgta tcactgtaat aaagggtgaa cacgtgcgat tagtgtattt ttatttcaaa
     6181 ttatgaaaac gttttaatca taaattgctc aattaaatta tttaatgata tactacagtt
     6241 aaataaacag tgataagggg agaaattgaa tgtcagtaag aattgaacat gatacttttg
     6301 gagaaataga agtacctgca gataaatatt ggggtgctca aacagaaaga agtaaacgca
     6361 atttcccagt tggtaaagag cgtatgccaa tcgaagtagt ttatggtttt gcacaactaa
     6421 agcgtgcagc agcattagct aattttgatt taggaaaatt aagcgaggca aagaaagatg
     6481 ccattgtata cgcatgtgat caaattttat caggtgaatt agatgaacac ttttcactag
     6541 ttgtatggca aacaggaagc ggtacacaaa gtaatatgaa tgtgaacgaa gtagtaagtt
     6601 atgttgctaa tatgtattta aaagatcatc aaattgatga aagtatccac ccaaatgatg
     6661 atgtaaataa atcacaaagt tcgaatgata cattcccaac tgctatgcac gttgcattat
     6721 atcaagaggt tgaaacaaaa ttagaacctg cattaaaact tttaagaaat actttgaaag
     6781 aaaaagaaga taaatttgat tcaattatta aaattggtcg tacacattta caagatgcaa
     6841 cgccgatcaa actaggacaa gagattagtg gctggcgtta tatgcttgac cgttgcgaaa
     6901 caatgttatc tgaatctaag aagcacattt taaatcttgc catcggtggt acggctgttg
     6961 gtactggtat taatgcgcat cctgaatttg gtgataaagt ggcacattat atttcagaaa
     7021 atacgggtta tccatttgta tcttctgaaa ataaattcca tgcattaaca gcacatgatg
     7081 aagtagtaca actacatgga acattgaagg cgctagcagg agacttaatg aaaattgcta
     7141 atgatgtgag atggttggct tcagggccac gagctggttt ggcagaaatt tccatccctg
     7201 aaaatgaacc aggttcatca attatgcctg gtaaagttaa tcctacacaa tgtgaaatgt
     7261 taacaatggt tgcagtccaa gtaatgggta atgatacagt tgttggcttc gcaagttcac
     7321 aaggtaactt tgaattgaat gtttataaac cagttattat gcataataca ctacaatcaa
     7381 tttacctttt agctgatggt atggaaacat ttaataacaa ttgtgcagtg ggcattgagc
     7441 caatcgaaga gaatattgat aattatttaa atcaatcatt aatgttagtt actgcattaa
     7501 atccacatat tggttatgaa aaagcagctc aaattgctaa gaaagcccat aaagaaggtt
     7561 taactttaaa agaatctgca attcaaactg gatatgttac agaagaacaa tttgaagcat
     7621 ggattaaacc agaagatatg gtagatcctc attaattaaa tattaatata aaataaagtt
     7681 tgggcattat agtgttcaag ctttattttt tgtctatgtc aaattgaact tataagtccg
     7741 atttgatata gagccatgaa aaggaaaaac ttttcatgga gtccgtttta ttatagaacc
     7801 tattttttat atgatgaatt gttgtacatg tattggccaa acaacctgtg ataaaaatct
     7861 tatgatgtca tgtattgtcc atagatacac gtaacgcagt tgaaaaagac tacaagtcat
     7921 tatccaagga tgttataaaa tgaataattg ctaaaatatc aaaatataaa atcctaagca
     7981 tcgtaaaaaa caaaaaaacc gaacatctga aaaaattaat aattttccat ctatccgatt
     8041 taagttgata cttgatttag ttacaatgcg gtattgcatg tttaaatatc atgcaaaact
     8101 aatcaaatta gtcatccaaa gaaacggaaa tgacttcctt agtcaaggga tgcacaaact
     8161 ctattttgta actttcaagt tctaattgtc ttaacgttga atcaccatat aaaggatcac
     8221 caataacagg gtgaccaatt tcagctaaat gcacacgaat ttgatgagta cgtccagtat
     8281 ccaacttgat ttcaagttgg cacacagctt cttttatcat ttttgaagtt aaaatgtgtg
     8341 taattgcacg ctgtccagta ggagacacac gtcgtttatt cgaatggaat ttatctttac
     8401 caattggcat atcaatcgtt tgtggtttta aaggtaaaag tgccttaaca tttgctttgt
     8461 atatccgcgt aatatcattg tcttctaaca tgcgatcaag aatttttttc attaaaggat
     8521 ttttagcaac aattaataat cctactgttt cctggtccag tcgatgaatt ggttcgacat
     8581 agtcactatc aatagtgtaa atcacatgat tcattaaagt attgctttct tttaaatcat
     8641 tagggtgagt cttaacacct ttaggtttca ttacgattgc catatcatcg tcttcgtatt
     8701 taatttgtgc ataacgataa cttggaacat aattactttt ttcatcgatg gttggaacaa
     8761 atacatcgtc gccagtatgc actttatcca ttaatcgcgc aggtttatca ttaatagtaa
     8821 tatctttaga catatttaaa tgatgtaaat cttttttagg taccttaagt tgttggaaaa
     8881 tatctcgtaa acttaagtca ttaaagtttt ctggtatttt aaatttcata aaatcctcct
     8941 atagtcttta atatcatatc acaaatcagt tctccgaaat ataactattg agtaatcaag
     9001 gccattttat catgtcacgt ctaagattta aaaatataat aactcaatgt ataatagtta
     9061 atacacgtaa aaatgaattg gagcgatttg atggaacaaa ggacgcgact agctttatta
     9121 aaagagattg ccgaattttt aaacgaagaa actgaaatgt atagcatgac ccaaggtgcg
     9181 ttaaaatatt taattgaggg cagtaatttc acgacaggct ggatattttt catcaatagc
     9241 gtaggtgaac atgaacttgt atcacatgtg gctctaccac aatctttgac agcagaccac
     9301 tgtcactata ttaaagatgg gtcttgttgg tgtgtgaaag catttaatca acgtaggcta
     9361 atgaaagcgt cgaatatcgt caattgttct cgtattaact tagcctctaa agcatttcct
     9421 agccaaaatg acaatattac acatcatgca acggtaccac ttaaatcggg tcaagaacaa
     9481 ttcggcattt taaatgtagc gtccccaaat actgaaatat atagtgatga agatttagag
     9541 ttgctagagt ctgtcgcatt tcaattaggg tcagccatca aacgtattta tttaacagac
     9601 cgtgaaaaag aagcagcaaa aattaatgaa cgtaatcgtt tagctagaga tttacatgat
     9661 tcagtaaatc aaatgttatt ttctgtaaaa ctaacagcac atgctgcgta tggcatgtca
     9721 agcgaatcca tcgctaaaca agcattcaag acgattgagg aaacgagtca aaatgccgtt
     9781 aatgaaatgc gtgcattgat ttggcaactt aagccagttg gacttgagca aggtttaatt
     9841 catgctttga cagcatatag taaattaatg catatacaat taaatgtaaa tgtagaaggt
     9901 ttaatcgatt tatctaatga aatcgaagaa aacatatata gagcattaca agagtgtatt
     9961 aataatgtta agaaacatgc tgatacgaat aaaatggatc ttacattaaa acaaatgaac
    10021 gatatactat atattgacgt gattgattat ggtcaaggat ttgagatcga taatgttcag
    10081 atagcttcat cacacggtat taataacata aaacaaagag ttaaattatt aagaggtaaa
    10141 gtgacctttc attctcagcc aacaaagggt acacaaatac aattcacaat tcctataaaa
    10201 tagcttgatt taaggagata acccatgaac aaagtaatat tagtagatga ccattatatt
    10261 gtgcgacaag gattgcgatt tttattatcc acgattgaaa acatagaagt tttacaagac
    10321 tttgcagatg gagaaacatt tttagaatat ttaaaagagc atgagcaccc tgatattgtg
    10381 ctattagatt tagtgatgcc tggcatgaat ggtattgaaa ttacggaata tattaaggca
    10441 cattatccgg atattaaagt tttggtatta acaagttatg ttgatgatga acatgtaatt
    10501 tcagcaatca ataaaggtgc tgatggttat gaaatgaaag acgttgagcc tcagcaatta
    10561 attgaaacta ttagacgagt tatgaacggt gaaaaaatga tacatcctaa ggcacaagat
    10621 gtattcgaaa cagttagcca aaaaccacac tacacgaata agttgtcaaa gagagaaatt
    10681 gaagtgttac gtgaaatggt taaaggtaaa acaaataaag agattgcaga aactttattt
    10741 gtatctgaaa aaacaattaa aacacatgtc agtcatatat ttagtaaatt acaagttagc
    10801 gatcgtacac aagcagcaat ttatgcaatg gaaaataagt tgatttagaa tgagctttta
    10861 aatatttgtc gaaaaataga caatttttaa tctgattttg atgaattttt tatagcgata
    10921 gggtttatta tacagttttc tttgtaaatt tgatatcatt ataaaagtgt aagttttatc
    10981 aaatctagtg attatgcata aaattttact tcatttactt tcgcttcatt ataaagtaaa
    11041 cttaactctt gttgacttga tcaaaattta atacaaatgt gaaattgact cttttcattt
    11101 tattaaaaat atattaagat gtaatctgtg tttcaagaaa tgtgtattat tgcaatttct
    11161 tgtgtccgga taaaattccc caaacagaaa ggtagataac agaatggata gacagagttt
    11221 tacagattta attcaaacaa aatttaaaat ggttcgtata gaggctggtt atacgcaaga
    11281 tactatggcg caaacaattg gactttctaa aaagacttta gtacaaatcg aaaaagagag
    11341 agtattacca aactggacaa cttgtatttc aatttgtgca ttattcagag actctgaagt
    11401 attaaacagt acatttggct gtgatccatt agaaatcgtt caaacaattt caagaaatca
    11461 ctgtgcatat ccaaaccatg caccaacaag tgatatttat tggaataaca ttgagactcg
    11521 taatgggtac attttacaaa gtaacaaagt aagcaatatt tatcgtgtat taaatccaga
    11581 taaccaacca attttcggta cttctaaaat gagagaagct gaaacatact ttaacagaaa
    11641 tgctaaagaa gaattagtac atatctaggc ctatatactt ttaataaata ataaaagcga
    11701 tgtaacacac ctgtttcgag aggtgaggca tcgctttttg tatgtaaact aataataaat
    11761 tgagcagaaa gtgaaatgat taatgaatta aaaaataaaa atagtggtat aatgaataac
    11821 tatgttgtca caaaggagtc aaaattatga atgcactatt tattatcata tttatgattg
    11881 tagtaggggc gataattggt ggcattacta atgtaattgc aattagaatg ctgtttcacc
    11941 cctttaaacc atactatata tttaaattta gagtcccatt tacaccaggt ttaataccga
    12001 aaagacgcga agaaattgca actaaaattg gccaagtgat tgaagagcat ttgcttacag
    12061 aaactttaat taatgaaaaa ttaaaaagcg agcaatcaca gcaagcaata gaatctatga
    12121 ttcaacagca gttacaaaag ttgacgaaag atcaattgtc aataaaacaa attacttctc
    12181 aaatcgatat tgatttagaa caagtattac aaactaatgg aaatcaatat attgaatcac
    12241 aattgaataa ttattataca aagcatcaaa accaaacaat agcatcttta ttgccaaatc
    12301 aacttgtaac atttttagat cagcatgtag ataacgcaac agacttatta tgtgatcgtg
    12361 caaggaatta tttatcatct gcaaaaggca cacaagatat taatgatatg ttggatactt
    12421 ttttcaatga gaaaggtaag ttaattggta tgttgcaaat gtttatgaca aaagagagca
    12481 ttgcagatcg cattcaacaa gaacttatac gtttaacatc tcatcctaaa gcaagaacaa
    12541 ttgtgacatc gttaattacc aatgaatatc aaacttttaa agataaacca ttgaatgaac
    12601 tgttagatgc gtctcagttt aatgaaatcg ctgaaaatct atcagtgtat ataacaacat
    12661 atgcaagcaa acaagcgaat aagccagtgg ttacgttaat gcctcaattc gttgattatt
    12721 tagaaggtca actctctagc aaattagcaa atttaatcat tgaaaaatta tcgattcact
    12781 tatctagaat tatgaaaaaa gtggatttac gaggattaat tgaagaacaa attaatacgt
    12841 ttgacttaga ttatattgaa aaattaatta tagaaattgc taacaaagaa ctaaagttga
    12901 ttatgtcatt aggtttcatt ttaggtggta ttattggatt tttccaaggt ttggttgcaa
    12961 tctttgtcta aaaaagcgag atgtggtaaa gtaatatcga acgttttaat aaacatcaca
    13021 acaaaggagt gctataacca tggcagtaaa tttatatgat tatgcaaatc aattagaaca
    13081 agctttaaga gaaagcgaag aatacaaagc aatcaaagaa gcattcgcta atgtaaaagc
    13141 taacgaagaa tctaaaaagt tattcgacga gttccgtgaa actcaaatta acttccaaca
    13201 aaaacaaatg caaggtgaag aaattgctga agaagattta caaaaagcgc aagaacaagc
    13261 gcaagcaatt gaaaaagatg aaaacatctc tgcattaatg aatgctgaac aaaaaatgag
    13321 tcaagtattc caagaaatca accaaattat cgttaaacca ttagacgaaa tttacgctga
    13381 ctaatattaa aataatattt ttcaatcctt gtaaatagag gaatagactg ccgacagagt
    13441 ccgagacttt gtgggtagtt tttttagttt tgataacgga agttagaggc tctctgtcaa
    13501 attgggcaaa tgcgttcagt atcggaattt aggctactaa acatgtccta gctttagctc
    13561 agaatggacg acggaagctg ggcaatcaaa catggctagc tctatgccaa aacgggcaga
    13621 tgagcttgat aacggaagtt aggctagtaa acgtgtccta acttcctttt tgcattttga
    13681 agtgtaaagt tttcaacata atactattag ttcggtcatg tatcggactg atggaaaagc
    13741 gtttcacttt taatgactca ttaagaacgg cctgaaaatg tttggcgtat taagtgcaat
    13801 gatagttttg acatttagtt tctaattggt cattactgcc gagcaaatct agtagagtaa
    13861 tcatgtaaat ctttaatgtg ccatttgatt cactagcggt gttaataact acggaaattg
    13921 catttccgac tgaaattttt gaaaaatatc aacgtacgct acaaataaaa ttttaaactg
    13981 ttataaatgt gtctcaattt catatgttca tcgacgatat gaagcgtatt atggtaaaat
    14041 gaagaaataa taaacttgtt aataaataaa acatcacgat ttgactaaag cactttatta
    14101 ttgtgtagat aatagttttt taacgaaata aaaatggcga ctggttttaa taaatcagct
    14161 aatgaatcac tacacctata agtatgaata tagtgattag aatgctttgt atagttggat
    14221 tttgcaaaat tgatgttaga acctttaatt aaggagatat ggatgtatgg ttaaatttat
    14281 tcattgttcg gatttacatt tagatagtcc tttcaaatct aagagtcata taagccctaa
    14341 aatttttgag gatgttcaaa aaagtgctta tgaaagtttt aaaaatattg tagatattgc
    14401 attacaacaa gatgttgatt ttgtaattat cgcgggtgat ttatttgata gtgaaaacag
    14461 aacattacgt gctgaaattt tcttaaaaca gcaatttgaa cgtttacaaa atgaacaaat
    14521 ctttgtttat gtttgtcatg gtaatcacga tcctttatca tcaaagattt catcaaactg
    14581 gccagataat gtttctgtat tttcaaataa agttgagacg tatgaagcaa ttactaaatc
    14641 tggtgaaaca atttatattc acggatttag ttatgaaaat agagcaagtt atgagaacaa
    14701 gattgatgaa tatccatcaa gtcaaggcca aaaaggcata catattggtg tcttgcatgg
    14761 tacgtatagt aaatcttcag ttaacgaaag atataccgag ttcattttag aagatttaaa
    14821 cagtaaattg tatcattatt gggctttagg tcatatacat gaacgtcaac aattaagtga
    14881 tatgcctgta attaactatt caggtaatat tcaaggtaga cattttaatg agcaaggtga
    14941 aaaaggttgc ttattaatcg agggtgacca cttaaaatta aagactaaat tttatcctac
    15001 acagtatatt agatttgaag aagcaactat tgaaacggat aagacatcta agcaaggttt
    15061 atacgaggtc attcaaaact ttaaagaaca agtgagagaa gaaggaaaag ccttttatcg
    15121 tttaacgctt gttattaata gtgagacatt aatttcacct caagatttat tacaagttga
    15181 agaaatgatt acagattatg aagaaaacga aaatcaattt gtatatattg atgagttaaa
    15241 aatacaatat gcacaaaatg atgagtcacc tttagttaat gaattttcag cggaattatt
    15301 agtcgatcaa gctgtttttg ataaagcgat gtcagattta tatttaaatc caagggcatc
    15361 aaagttccta gacgattatg gaacattcga ccatacagca ttagttaatc gtgctgaaga
    15421 aatattaaaa gctgaaatga gaggtgaaca aaatgataat taaatcactt gaaatttatg
    15481 gttacggtca atttgttcaa cgtaaaattg aatttaataa aaacttcact gaaatttttg
    15541 gtgaaaatga agcgggtaaa tcgacgattc aagcattcat ccattcgata ttatttggtt
    15601 ttccaactaa aaagtctaaa gagccaagac tagaaccacg tctaggtaac caatacggtg
    15661 gtaaattagt acttattctt gatgatggct tagagattga agttgaacga attaaaggca
    15721 gtgctcaagg tgatgtgaaa gtatatttac ctaatggtgc tgtgcgtgat gatgcttggt
    15781 tacaaaagaa acttaattat atttctaaaa agacatatca aggtatcttt tcatttgatg
    15841 tactagggct tcaagacatt catagaaatc taaatgaaaa acaattgcaa gattatttat
    15901 tacaagcagg ggctttagga tcaactgaat tcacgtcaat gcgcgaagtg attaatcgta
    15961 aaaaagatga attatataaa aaatcaggta aaaatccgat cattaatcaa caaattgagc
    16021 aattaaaaca actagaaagt caaattcgtg aagaagaagc taaactagaa acatatcatc
    16081 gcttagtaga tgatcgagat aaatcatcac gtcgattaga gaatttaaag cataatttaa
    16141 atcaattatc aaaaatgcat gaagaaaaac aaaaagaggt tgctttacat gatcattcac
    16201 aagaatggaa gtctctagaa caacagttaa atattgagcc aatcacattc ccagaaaaag
    16261 gtgtggatcg ttacgaaaaa gcacgagcgc ataagcaatc gttagaaaga gatattggtt
    16321 taagaaatga gcgtttagct caacttaaag aagaagcgac tcaattagag ccagttaaac
    16381 aatctgatat tgatgccttc attagtttga atcaacaaga aaatgaaatt aaaaataaag
    16441 aatttgaact tactgcaatc gaaaaggata ttgcgaataa acaacgtgat aaagatgaac
    16501 tgcaagcaaa tattggttgg tctgaaacgc atcatgacgt agatagttca gaggcaatga
    16561 aaagttatgt cagtgagcaa atcaagaata aacaagaaca agctgcatac attaaacaat
    16621 tagaacgtag tttagaagaa aataaaatcg aagataatgc ggttcatagc gaactagatt
    16681 ctgttgaaga aaaaatagtt cctgaagaga cttttgaaaa gaaaaaagaa tactcacaac
    16741 aagtcattga attaaacgaa aaagagaact tgtatagcaa attgaaagaa cgtttcgaaa
    16801 ttgagcaaca agaaaaacaa aaacgtcaaa aattgttacg aacaacgttt attcttttaa
    16861 cattagtagg tattggttta actgccttct catttatttc aaacaacatg ttgtttggta
    16921 ttattttcgc agtattaact ttagtatttg ttatcggtat catcatgtct aaatcaaaag
    16981 aagtagatta tagcgaggcc atcacagatg aaatcgaaga aattaaagca caactagcca
    17041 ttttagatga aaattatgac ttagattttg atcttgatga gcaatatcgc attcgtgatc
    17101 actggcaaca agctttgaaa aataaagata ttttagaaga gaaacgtcag tatattgaag
    17161 gtcgtttaaa tgatgcgaaa gggcgtcatg atgaattaca atcgacagtt gagaacgtta
    17221 aggatgaatt atacttatca tctaaaattt caaatgattt aattgttgat agtatttcta
    17281 caatggcgaa tattaaagca ttagatcaac atatcagtga tttaaatcaa caacgccagc
    17341 aattggttca agaattagat acattctaca atcatgctga agctgttaca aaatcacaat
    17401 ttgtttattt caacaaatta tctttattcc acgatgtgca acaatggttg aaatcagctg
    17461 aagacacgaa tgaaaaatgg cgtattaatg ctgaaaatac caaacttgtt acaaatgaat
    17521 taaatcattt aaatgctcaa ttagaagaaa ataataaaga aattacagca ctattcgatt
    17581 ttatcaatgt tggtactgaa gaagattttt accaacatca tgaagattac caaacttata
    17641 ctagtaattt gagtcgtttt aatgatttaa ctaaatatct tgagaaccaa aactattctt
    17701 atgaattaag ttctagttta agtgaaaaaa caactgcaca acttgaagaa gaagatcatt
    17761 tattggctac tcaagttgac gaatataatg agcaatatct tgaaatgcaa gcacaagtca
    17821 gtgatttaag tgcacaaatc aatcacatgg aaactgatac aacgcttgct aatttaagac
    17881 atgaatatca cagtcttaaa aatcaactta atgatatcgc aaaagattgg gcaagtttaa
    17941 gttatttaca aagtttagtt gatgaacaca ttaaacaaat taaagataaa cgtttgcctc
    18001 aagttattaa tgaagcggta gaaatattga agcatttaac agatggcaga tatacgatga
    18061 ttaactataa tgaagattca attacggtta aacatgttaa tggtcaatta tatgatcctg
    18121 ttgaactaag tcaatctaca aaagaattac tttatgtagc tttacgtatc agtttaatta
    18181 aggtactaag accatattat ccgttcccat taattgttga tgatgcattt gttcattttg
    18241 ataaaaaacg tactgaaaaa atgttgaatt atttacgatc attatcagaa cactatcaag
    18301 tactatactt tacatgtgta aaagataata ttgttccatc aaaagaagtg attacattaa
    18361 acaaaataga ggaaggcggg aaacgatgag aaatatagag aatctaaatc ccggagattc
    18421 agttgatcac tttttcttag tgcataaagc tacacagggt gtaacagcac aaggtaaaga
    18481 ttatatgaca ttacatttgc aagataaaag tggtgaaatt gaagcgaaat tttggacggc
    18541 tacaaaaaat gatatggcaa caatcaagcc tgaagaaatt gtacatgtta aaggtgacat
    18601 cataaactat cgcggaaata aacagatgaa agtcaaccaa attagactag cgacaactga
    18661 agatcaatta aaaacagaac aatttgtaga tggtgcacct ttatcaccgg cagaaataca
    18721 agaagagatt tctcattatt tgctagatat tgaaaatgct aatttacaac gtatcacacg
    18781 tcatttattg aaaaaatatc aagaacgatt ttacgcatat ccagctgcta gttctcatca
    18841 tcataacttt gcgagtggct taagctatca tgtattaacg atgttacgta ttgcaaaatc
    18901 aatttgtgac atttatccat tgttaaacaa aagtttgtta tatagtggta ttattttgca
    18961 tgatattggt aaagttagag aattgagtgg tcctgttgcg acgtcgtata cagtcgaagg
    19021 taacttatta ggacacatct cgattgcgag tgatgaagta gttgaagcag ctcgtgaatt
    19081 gaacattgaa ggagaagaaa tcatgttgtt aagacatatg attttatctc atcatggtaa
    19141 gttagagtat ggttcgccaa aactgccata cttaaaagaa gcagaaattt tatgctatat
    19201 cgataatatc gatgctagaa tgaatatgtt tgaaaaggct tataaaaaaa ctgacaaggg
    19261 tcagtttaca gataaaatat ttggtcttga aaatcgtaga ttctacaatc ctgaatcact
    19321 cgattaagaa ttaaaagata tcggacagat gagtatactg aaacatgagc tcaactgtcc
    19381 gatttttaat gcgaaaaaac cgagcggccg tggtatgatt tttgaaccac ggtcgctcgg
    19441 ttttgtgtta ttggctcatg ccggattgtc cgccttgtgc gccaccttgt ttaagttttt
    19501 cagggtttaa gattttatct tcgacaactg atttaatatc acgatcttta aagtcaacat
    19561 cgtattcttt taatagatct ttgtatgcat cagtcaataa ttttggattt ttttgtactt
    19621 tctgatcgac taatttttct ttcaggcttt gtttttcact gttaaagtct gttggtttat
    19681 cagctttaat aatatgatat ccaaagcttg atttaacaac ctctgatact tcaccatctt
    19741 taagcttaaa tagtgctttt tcaaaatctt tatcagtttg tcctttaaga acataaccta
    19801 attcgccatc ttttttagct gaaccagtat ccattgattc ttttttagcg atttcaccaa
    19861 atttacttgg atcttttgaa acttcttttt gaatttcttc agctttttgt ttcgcttctt
    19921 tatcgtctaa gccttctttg tcgcttttct tagatttaac tttaattaaa atgtgtgaag
    19981 ctttcttgct gtcttcttta atttcagaat cagagatttt aattttatct gatagtaatt
    20041 ctttatgata agcagcagta cgtaaatttt ctttatattt atcggctgtt aaaccttgct
    20101 gttgaagggc cttttcaaat ttatctttac cgccgtattg cttttgcatt ttttcaattt
    20161 gttcgtcaat cttcttatca ttaactttat ttttatattt atcagctaaa attttattta
    20221 acatttcagt aaatgatgca tttgcaattt gatctttacc gatttttttc attgtatctg
    20281 caactgttac atctccagct ttagaagaaa ttaatgtatt ttctttagag tctgtggcac
    20341 tagcgccaca agcgcctaat aataaagcac tagctgttac cggaacgatt aatttgttta
    20401 tcatcttcat agttgaaact cctttgtaag ttaatcatcg tgaatataac atagttttat
    20461 attatcacca atcaataacc catgacatca gactagagca ttaggtttag gggtagaaat
    20521 tgatgaaaat aaaaaaacaa ggtctatata catttgattg taagctatt
//
LOCUS       DAHHYJ010000032        20534 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000032 DAHHYJ010000000
VERSION     DAHHYJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20534
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.032"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(4..603)
                     /locus_tag="NBK22_000900"
     CDS             complement(4..603)
                     /locus_tag="NBK22_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD2414103.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            complement(879..1289)
                     /locus_tag="NBK22_000901"
     CDS             complement(879..1289)
                     /locus_tag="NBK22_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD2414104.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(1276..2139)
                     /locus_tag="NBK22_000902"
     CDS             complement(1276..2139)
                     /locus_tag="NBK22_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD2414105.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(2181..2966)
                     /locus_tag="NBK22_000903"
     CDS             complement(2181..2966)
                     /locus_tag="NBK22_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD2414106.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(3092..3982)
                     /locus_tag="NBK22_000904"
     CDS             complement(3092..3982)
                     /locus_tag="NBK22_000904"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCD2414107.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(3992..5338)
                     /locus_tag="NBK22_000905"
     CDS             complement(3992..5338)
                     /locus_tag="NBK22_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD2414108.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKMGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIEDRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            complement(5452..6552)
                     /locus_tag="NBK22_000906"
     CDS             complement(5452..6552)
                     /locus_tag="NBK22_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCD2414109.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQDVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGESNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNTEKINIDVEASTINTDPFQYI"
     gene            complement(6555..7232)
                     /locus_tag="NBK22_000907"
     CDS             complement(6555..7232)
                     /locus_tag="NBK22_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCD2414110.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCDYGI
                     AGEVIQGPFQAAVKNVAANQLVDRIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(7363..8469)
                     /gene="rpoD"
                     /locus_tag="NBK22_000908"
     CDS             complement(7363..8469)
                     /gene="rpoD"
                     /locus_tag="NBK22_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCD2414111.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNTSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(8693..10510)
                     /locus_tag="NBK22_000909"
     CDS             complement(8693..10510)
                     /locus_tag="NBK22_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD2414112.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYG
                     GYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVF
                     EVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
                     GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(10571..11389)
                     /locus_tag="NBK22_000910"
     CDS             complement(10571..11389)
                     /locus_tag="NBK22_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCD2414113.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(11400..12023)
                     /locus_tag="NBK22_000911"
     CDS             complement(11400..12023)
                     /locus_tag="NBK22_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2414114.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRRFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            12358..13749
                     /locus_tag="NBK22_000912"
     CDS             12358..13749
                     /locus_tag="NBK22_000912"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCD2414115.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(13898..14644)
                     /gene="recO"
                     /locus_tag="NBK22_000913"
     CDS             complement(13898..14644)
                     /gene="recO"
                     /locus_tag="NBK22_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCD2414116.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYASLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(14672..15571)
                     /gene="era"
                     /locus_tag="NBK22_000914"
     CDS             complement(14672..15571)
                     /gene="era"
                     /locus_tag="NBK22_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCD2414117.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(15572..15976)
                     /gene="cdd"
                     /locus_tag="NBK22_000915"
     CDS             complement(15572..15976)
                     /gene="cdd"
                     /locus_tag="NBK22_000915"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCD2414118.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(15987..16331)
                     /locus_tag="NBK22_000916"
     CDS             complement(15987..16331)
                     /locus_tag="NBK22_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD2414119.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(16334..16801)
                     /gene="ybeY"
                     /locus_tag="NBK22_000917"
     CDS             complement(16334..16801)
                     /gene="ybeY"
                     /locus_tag="NBK22_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCD2414120.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSGLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRG"
     gene            complement(16802..17749)
                     /locus_tag="NBK22_000918"
     CDS             complement(16802..17749)
                     /locus_tag="NBK22_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCD2414121.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKIDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
     gene            complement(18053..18751)
                     /locus_tag="NBK22_000919"
     CDS             complement(18053..18751)
                     /locus_tag="NBK22_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCD2414122.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQPETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     GMIWSEILAKPKQL"
     gene            complement(18768..19757)
                     /gene="floA"
                     /locus_tag="NBK22_000920"
     CDS             complement(18768..19757)
                     /gene="floA"
                     /locus_tag="NBK22_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCD2414123.1"
                     /translation="MFSLSFIVIAVIIIVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            complement(19775..20458)
                     /locus_tag="NBK22_000921"
     CDS             complement(19775..20458)
                     /locus_tag="NBK22_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD2414124.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
BASE COUNT         6421 a         3831 c         2790 g         7492 t
ORIGIN      
        1 actttatttt gttgcattat ataattcgtc aactttttcc cagttaacta cattccaaaa
       61 tgcgccaatg tagtcagggc gtttgttttg atattttagg taataagcgt gttcccatac
      121 gtctaaacct aaaataggtg ttttaccctc agttaatgga ttatcttggt ttggtgtagt
      181 cacaatttct aactggccat tgtttactac taaccaagcc caacctgaac caaagcgtgc
      241 agctgctttg tcagcaaatt cttttttaaa ttcttctaaa gaaccccatt gttctttaat
      301 tttttctact acagtacctt tttcttctga gtttggtgaa agtaactccc agaataatga
      361 atggtttaaa tgtccaccgc cattattacg tacagcagtt tggatgttag ctggtacact
      421 gtctaaatta gcaacaattt cttcaataga tttagattct aaatctgtac cttctactgc
      481 agcatttaat ttcgtaacat acgtgttatg atgtctgtca tggtgaattt ccatagtttc
      541 tttgtcaaaa tgtggttcta atgcatcaaa tgcgtatggt aattttggta attcaaaagc
      601 cataaataat catcctccta aaatgtctgt aagtaaataa taacaagcag tgactggttc
      661 aacaaagaat ttgcttaaat tctactactt attattttct ctactcattt aatataactc
      721 aaatcaaaaa taattaaaca ttttgtatat aaaaagttaa cagatttgcc ataaaatcat
      781 acgaacggag tatgaaatga acctttatct tctataattt aaaaaatgag atttatgcat
      841 acatctgacc aaatgtgcat aaatctcatt tctttatatt aatcttggca agactcacat
      901 acaccataaa cttcaagttt gtgtttgtga atattaacac caggtagtga taactttatc
      961 tgatctattg gacaataatc tattaccttt gtatctccac acttttcaca gataaaatga
     1021 tgatgatgat ggtttgtaca agcgattcta aacttcattt caccatcaag ttctgtattt
     1081 tcaataatcc ctaaatcttt aaataagtgc aggtttctat atattgtgtc gaatgaaatt
     1141 ccaggataat tttcatccat aacttgctgt atatactttg cgtttatata cttatcttct
     1201 tcgacaaaaa tatctaacat atctttacgt ttatctgtat attttaaacc gttctctttt
     1261 aaaattttaa tagcatcatt tgtattcatt gatattagct ccctttttaa acttcattcg
     1321 cattttctga taagccattg taatcataag taaaataaca agtagaacta caattacacc
     1381 acccggagaa atgtccatat agaaagctag gactaagcct aatattactg ataattcacc
     1441 taaaaataca cttagtaata tcaattgctt aaaacttttt gttattcgca tacttattgc
     1501 aattggtaac gtgattaacg cacttactaa cagtatccct acaacacgca ttgaggcaga
     1561 aataaccatc gctacaataa caataaataa aaattgaatc catttaggaa tgccaatgac
     1621 tttactatat tcctcatcaa atgacaatat aaataattct ttataaaaca atgtaataaa
     1681 cagaacaact atgatggcaa tgacaataat cgttgttaaa tcacttatat tcactgcgct
     1741 tattgagcca aatagcaatc caacaatttc ttgattgaac ccatcagcta atgaaatgaa
     1801 gattgcactc aaggcgatac cagcactcat tataattgga atagcaattt cttggtaagc
     1861 agtgtatgac gttcttaatt tttcaattag aagcgcacct actattgcga ataagattcc
     1921 aaaccacatt ggattaataa atactagtgt tggcataata gtaagtaaaa acataccgaa
     1981 agatatacca cctaaagtta catgacttag agcatcagct ataagtgata gtcgtctaac
     2041 aacgataaaa gcaccgatta gaggcgcaat aaaacctatc aagataccac taattaaaga
     2101 gtacctcata aaatcaaaat tcaataatgc atctatcaat tgtgacacgc ctttccattt
     2161 taaataaact caaatcttta ttaattacaa cattctcgat tatgctgatg atcgacaaaa
     2221 cgtacaggat gtccataaat ttttgaaatt tcaacttcat caagtgattt aaactcatca
     2281 gttgtaccat ggaaatgcaa atgcttattt aaacatgcta cttcagtagc agtatctgct
     2341 acaacaccga tatcatgagt aactaagata atggtgatac cttcttgttt taattgatct
     2401 aaagtattat aaaattcact tacatgtttt gcatcaatac cattcgttgg ttcatcaagt
     2461 actaatactg caggttctga aatcaatgct cgagcaatca ttacacgttg ttgttgacca
     2521 cctgataatt ctgctatatt tttatgaatt aaatcactta tattcagtct ttctagtact
     2581 ttaatcactt tttcattatc tttgctatta aatgtttgga aaagacgttt tgtctttgtt
     2641 aatccgctta aaacaacttc tttaacactt gctgggaaac ctgaattaaa ggcatttgct
     2701 ttttgtgata catagcttaa tttaattgat gttttcttat ttttaaaatc aataccttca
     2761 acaaaaatct caccactttg taaaggtaat aaccctagaa tcaacttcaa taatgttgat
     2821 ttaccagcac catttggtcc aacaattgct aaaaattcac ctttatttat tttaatgttt
     2881 atattttcta acactttttt atgatcatag tagtaattga catttttcaa ttcaaagact
     2941 ggtgtcatcg tattctcacc tcgcattcaa ctatacaact cctagtaaca tatgtaaaca
     3001 gtaatgttta cgactcaaaa ttagacaaaa taaagagata tgcccccttc aagttttatt
     3061 tatcgcattt cttgaagaga gcattatcat tttattgttg cataacctta ttttttaatt
     3121 ctgggtcaaa ttgctgttgt tttaacattt caatttcaag tttatatggc ggttttttat
     3181 ttttcttatc ttcaccaaca taaggtgttt ctaagatttt cggaatatct ttaaaactat
     3241 catgatgcac aatgtaattt aatgcatcaa aaccaatgta accgaagcca atattttcat
     3301 gtcggtcttt ttgagcgcca cggtcatttt tagaatcatt gacatgaaca actttgattc
     3361 tgtcgactcc aataatttta tcaaattcat ttaatacgcc atcaaagtcc tctttcacat
     3421 tatatccagc atcatgcgta tgacatgtat caaaacatac tgataaacgt tcgttattat
     3481 gaactccatc aataatacgt gctaactctt caaacgagcg accaatctct gtacctttac
     3541 ctgccatcgt ttcaagcgca atacgtacat tattgtcatt cgttaaaact tcatttaatc
     3601 cttcaataat cttattaatt ccggcatcaa caccagctcc aacatgcgca cctggatgta
     3661 atacaatatc tttagcgcct atagcttgcg ttctttcaat ttcttgttgc aagaaatcta
     3721 caccaagatt aaacgtttct ggtttggttg tatttgcaat attaatgatg tatggtgcat
     3781 gaacaacaat attagataag ccatattttt ccatcacttc atgaccttta gttatattta
     3841 aatcttcaat acttttacgg cgcgtgtttt gaggtgcacc agtataaatc ataaatgttg
     3901 tttcaccata ttcatgcgct tctatagcag aaccttctaa catcttttta ccactcattg
     3961 aaacatgtga tcctaataac ataaaacaca cctaaccttt tttgttttgc ttacgttttt
     4021 gtctattttg ctgcttacta aattgcttac gctcttgacg tttcattttt tcaacttctt
     4081 gtttaaattt cttcttataa cctggtttaa ctttgttttt aattttactt cgaactttgt
     4141 tcttcacttg attagttaaa tggtcatctt tgcgcattct tgcttgacgc tgattgtgcg
     4201 ctttaacttc ttttaactca ccatctttaa tatcaacagt attgaataca aaaccgcgat
     4261 cttctattaa tgaaatattg tgttcttcat caggactata aagcgtaatt gctacacctt
     4321 tataattccc acgaccagtt cgtccgactc tatgcgtaaa gaagtcaata tcatttggca
     4381 catcaaaatt gatgacatga ctaacacctt caatatcaat accacgagat gctaaatcgc
     4441 tggcaataac gtattggaat tctaaattac gtatacgttt catttgttgt ttacgttcac
     4501 gtggcgttaa accaccatga atcataccaa ctttaatacc agcttcattt agtgaacgtg
     4561 ctaaatcatt tgcattatct ctactattac agaaaataat acataagtat ggatttagta
     4621 tatcaattaa atttaaagtt ttttcaactt tagctgcacc tttagtaggt attaaaaaga
     4681 attcgatgtt ctttttattt tgttttttac tgtcgacagc tacatattct ggatgactta
     4741 aatatttatt taaaaatggt tgtaactgtt gtggaattgt agcactaaac accgcaatat
     4801 ttgcattatc ttccaatctt gcagcaatgt aatctacatc ttcaattaat cctaagtcaa
     4861 tcataagatc cgcttcatca ataactaaat atgatgctaa gtgcacatgt aaatgtccca
     4921 ttttagctaa gtcattaatt ctagtagggg tgcctataat caattgtggt tgtgcattac
     4981 aacgttgtct atctttctct atatctgtac caccaataaa aactttaact gaaacaccag
     5041 ctttaaattg gcttaaatgg ttcgctgcat cgtatagttg ttgtgcaagt tctcttgttg
     5101 gtgcaactac gattgcttgt ggttctttta tttcactatc aattaactgc attaatggta
     5161 ataaaaatgc atgagatttc cctgtacccg tttgagattg accaattaaa tttgttctct
     5221 ttagtattct tggaataatt cgattctgaa tttcagttgg tttttcaaaa ttaaggtctt
     5281 tcacagcgtc aattaaacta gattctagat taaattgttc gaatggatgt tttgccatga
     5341 tttggcctcc ttatatgttc atctttacta ttataaagta taaatgtgct attttaaata
     5401 aaaatattaa aacctcgaaa tttcaaaatt tagaaatccc gagattttag tttaaatata
     5461 ttgaaatgga tctgtattaa ttgtagatgc ttcaacatct atatttattt tttctgtatt
     5521 gaaccaattc attagtaacg tttttaaacc ttctttcatc acatattcgc tgtaatgatt
     5581 aatatcaatt aaattcacac catgaatttt agcatctaag gcatcatgat gtttaatatc
     5641 acctgtaaca aagacatctg cgccttgttg gacagcttga tattcatatc caatacctga
     5701 accaccaata attgcaatac gtttaatttt ctgattagac tcaccaacaa aacggacact
     5761 tgggatattt aatttagatt taatatcagc tgcgaaatct tccaatgtca tttgattatc
     5821 cacttctgcc ataacgccaa gtccataaag ggatgtttgt tttatctcaa taaaatcaaa
     5881 taccggtgtt tcatatggat ggtattgttt aattaattgc tcagcccttg acttttgata
     5941 tgcatctatc ataaattcaa ttttaacttc atctacatat tcaattttat caatttgtcc
     6001 tattgttgga ttagcttcac caactggttt gaattgccct cttccttcac tttcaaagaa
     6061 acaatattca taattacctt cttgcgctaa tccattttca ctaagcttat ctttaaatgg
     6121 tccaacatta tccttaggta tatatgtttg aactttatag tatacatctt gttgattatt
     6181 tattattgaa atgttcttca aacccatcgc cttcgccaac atcatattga caccatacgg
     6241 atttacatct aaatttgtat gcatcgctat taaattaatg tcatgttgaa ttagttttct
     6301 aatgatcaaa ccataaccat tagcttttaa tgatgttacg cctttaaaga ttagaggatg
     6361 atgactaata atagtattat aacctttttc gattgcttca tttactactt ccaacgtgca
     6421 gtctaatgct gttaaaacac cagtaacttc aacatcttca tcacctatta acaatcctac
     6481 attatcccaa gattcagcag tactaaatgg cacatgatga tctaacaatg tcattaaatc
     6541 agctattttc attactataa caccctttca attacagcaa tttcgtcatt aatttgagct
     6601 aaacgttgat gatgttgttc agtattgagt ttcgatttaa tatgataaag tgcttctaac
     6661 tctctttgcc atttttttat aaaatattca tttttgttgt tcagcaattt tggtccgaat
     6721 ttcaattcat cagatgatag ctctattaat tgtgtagaat attctgctac aacaatttca
     6781 taaatatggc ctttttcttc cattattatt tcatcaatta tttcataatt caattgttgt
     6841 aatgtttgtc ttaaattttc agtttgtata ttactttgta aaatcaacct tggatgttga
     6901 cttaacttat cttgcccatc ttttaaaatt ttagcaataa gtggtccgcc cataccacat
     6961 attgtgatat tatcgattac gtcctcagga tgaataacac ttaagccatc ccctaaacgt
     7021 acatcaattc tatctactaa ttggtttgca gctacatttt tcacagcagc ttgaaaaggg
     7081 ccttgaataa cttctcctgc aataccgtaa tcgcataaat ggttttgaat tgcatagatt
     7141 ggcaaataag catgatctga gccaatatcc gcgattgtac cttgttttaa aaatcgactt
     7201 accgtcgtta atcggttatt taacgaaatc attttattct cctttatgaa aaaagaggtt
     7261 gaacattatt caatgtacca acctcactca agttaaatct taatctaaaa ataaataagc
     7321 gcttattcat gtcttggtat caatcattaa aattgatatg aattaatcca taaagtcttt
     7381 caaacgttta ctacgacttg gatgtcttaa ttttctaagt gcttttgctt cgatttgtcg
     7441 aatacgttca cgtgtaacac cgaaaacttt accaacttct tcaagtgttc ttgttctgcc
     7501 gtcatcaaga ccaaatctta atcgtaatac attttcttct ctatcagtta atgtatcaag
     7561 cacatcttct aattgctctt ttaataattc ataagcagca tgatctgaag gactttgtgc
     7621 ttcctgatcc tcaataaagt ctcctaaatg actatcatct tcttcaccaa ttggtgtttc
     7681 taatgaaaca ggttcttgcg caatttttaa aatttcacga actttttctg ctggtaaatc
     7741 catttcttca ccaatttctt ctggtgctgg atctcgacct aagtcctgta ataattgacg
     7801 ttgaacacga attaatttat taattgtttc taccatatgc acagggatac gaatcgtacg
     7861 tgcttggtca gcaattgcac gagtgattgc ttgtctaatc caccatgttg catatgttga
     7921 aaacttaaat cctttgttaa agtcaaattt ttcaacagct ttaataagac ccatattacc
     7981 ttcttggatt aaatcaagga ataacatacc acgacctacg tatcttttag caatacttac
     8041 aactaaacgt aagttcgctt ctgcaagtct tgattttgct acttcatcac cttgttcaat
     8101 acgtttggct aattcgattt cttcttgtgc acttaataag ttaacacgcc caatttcttt
     8161 aaggtacata cgaactgggt catttatttt aacacctgga ggggcactaa gatcacttgt
     8221 attcagtttc tcgtcagtat ctgaactatc tttttcatta actagtgaaa tatcattatc
     8281 atttaattga tcaaagaaat catccatttg atcagagtcg atatcaaaat tctgaagttt
     8341 ttcagcaatt tcttcatgac ttaaatgacc ctctttttta cctttttcaa ttaattgctt
     8401 cttaacatct tctaatgtta atgtcggatc aattgtttgt tttttaattt taactgtgtt
     8461 atcagacatg aaacggcctc ccgattttaa atatgaacat tcgaaattta ttcaatattg
     8521 ctattttaaa ctaaattctt aattaattca tccatatttt taattttatt ttacaaattg
     8581 gaactaatcc caatatttat ttttcaatag tgttgtttaa atgcggtaac tatcatttac
     8641 ccttatacat tttgacataa tcattattcg tattattctt taaaatcaca tgctacatgc
     8701 gttctttatt cttagcaaca atttgctgta aatagtattt ttgtaattct acatcgccaa
     8761 tccttgtagc ttcccttaat ttatgattca atgactcaat tgtttcttgt cctttttcat
     8821 taataacatt gacataatca tcaatttcat tttcatatgg ttcgtcattc aaattatatt
     8881 gttctaagct aattagtgtt tctctcaact catttgaatt aacatactgc acagcatcac
     8941 tgatattata ttgatcattt tccgcataaa aatcatgtaa gacttcgaat acatatttaa
     9001 aatgctgatt tgtgaagtta tccttatcaa cactttcata ataatttaaa aatgtatctt
     9061 tatctctcat taaatgtttt aaaaatgctc gctccgcttt ttcttgacgg ctcaaattgt
     9121 caaattgtgc cataccaatt ggctcaggtt caatataacc gccatactca tcataaccgc
     9181 catactcatc ttctggataa taattggctg gtgcttgatt gaattgtatt tcgttagcta
     9241 actgctcagg actaacattg aaaaatggcg caacatcatt tatagccttt tgttgcagaa
     9301 ttgatgactt cataagtgaa atgtcatgac tcagttcttt caaataacgt tcatatgaaa
     9361 ggtcattatg tgcaatttca tcttttaata tacttacttt ataatgtgca aatgactttt
     9421 tgtcattttt tacaaaagta gtaaatgcgt cgttgccata cttaccaatg tattcatccg
     9481 gatccatgcc agatggcaat tgtataacaa atacatttag cccttgctgt aacaaatgtt
     9541 gacctgtttt aagtgttgct tcactacccg caaaatcccc atcaaacatt aatgttatat
     9601 ttgatgttaa ctttcgtata aaggtaatat gttcatctga caactgtgta cccattgttg
     9661 caacaacgtt tttcaagcca gcagtatcag attttataac atccataaaa ccttctagta
     9721 atacaatttc atctaatttt ctaattgatt tacgtgcttt atctaagtta tataacaact
     9781 ttcttttttg aaagataggc gtttcaggac tatttaggta ttttggttct tgaccggtat
     9841 atgttcgacc tgaatatcca acaattcttc cttgcgcatt tttcaaagga aacataatac
     9901 gatttcgaaa tctatcgtaa taactgaaat tttcttcgtt acgtgataat aatccggctt
     9961 catatgctaa ttcaatatcg taaccctttt tttgaagaaa atcatgacaa aaatgtgagc
    10021 tatcgggtgc aaagccaatg cctcgctctt taataagcgc atctgtaaaa ccacgttctt
    10081 gtaagtatgt taatgcttgt tcgccttcga ctgtctttgt taaagcgtaa taataaaatt
    10141 cttgtattaa ctcatgcatt tcaatcattt gtaaatcatc agaagcaatt tgaacatttg
    10201 agttagattg tgttgcctca atatctacag caacattaac tctatcacct aattctttaa
    10261 ccgcttcaac aaatgatatg tctttaattt cttgagtaaa ttgaaaaaca ttgccacctt
    10321 ttttacaacc aaaacaatga caaatttgtt tatcttcaga aactgtaaat gaaggtgtct
    10381 tttcatcatg aaaaggacac aaacctatat aattgcgtcc tctcttttct aattttacat
    10441 attcacttac caagtctaaa atgtcggttt tatcttttat ttcattaatg atcgattgat
    10501 ctattcgcaa attaaatcac ctattaagca ttagttatac tattatacct aattttcgac
    10561 aaaaatgaaa tcatttcgat ttattttgtt caatataatg gattatatcg tttgctgttt
    10621 cttcgattgc tttttgagaa acatcaatga caggacatcc aatttcactt acgatttctt
    10681 caaagtaatt caattcttct tgaattcgtg cttctgttgc atatcgagct gtgtcaccta
    10741 gtcctaattg ttttagtcgc tcttttctaa tgcgatttaa tttttcttca cttattttaa
    10801 gtgcgataca tttctttgga tcaatatcat ataagccatc tggcggtgtc acttctggta
    10861 caatcggtac attcataact ttgtaactct tatgcgctaa atactgagat aatggtgtct
    10921 ttgaagttct cgaaatacca agtaaaacaa tatcagcttt aggtaatcct ttaggatctt
    10981 taccatcatc atatttaact gcaaactcta tcgcatcaat tttcttgaaa tatgcatcat
    11041 ctaatctatg aacgatacct ggctcattat aaggtttttc ttcaaccgat gctgataata
    11101 aatccattaa tggccccatg atatcgacgg acttcaattg gaattctgct actttctcac
    11161 tcatatattg cttcatttca ggtttaataa gtgtataaac aatgatagca tttgtatctt
    11221 ttgcaacttg aatcacttca tcaacatctt caaaagattc aatatatgga tatcttaata
    11281 attcattttt acattgctta ggattaaatt gtgaaatacc tgcccttgca actaactctg
    11341 ccgtttcacc tatagaatct gaggctacga taattttaat tttttccatt acttctcacc
    11401 tattctttat ataatgctac taataactta gctattgttg ttttggaaat tcttccaatt
    11461 acttcatact tttgattatc tttttttctt acaattggaa tcgaatcaat ttctttttca
    11521 atcattctat ctgctgcgta tatgactaat tcgctttcct ctaaataagt gacattaggc
    11581 atacgtgtca tatttacact gataggtact gtatgaatat ctgctccaat cattgaagct
    11641 cttaataaat cttttcttga acacacacca acaaaatcgt tatcttcatt aataataaat
    11701 aatgtactta catcttctaa aaaaattgta caaatagcgt cataaactgt tgtattctct
    11761 cttagcacaa caggttgaga catatagtcc ttaacctcaa atcgtcgaag tttttcatta
    11821 aaaaatttac cttttgattt acctgaataa taatatccaa ctcggggacg cgcttctaaa
    11881 aaacctgaca ttgttaatat cgctaaatct ggtctaagcg ttgctcttgt taaattcaac
    11941 ttatctgcta tttgttcacc agtaatgggt cctttagttt taacaatttc gatgattcgt
    12001 tcttgtcttt gactgagttc tataggtctt cacccctttt ttattcactt attattatac
    12061 cgatatttct ttcatgtcac aaacacatta attttacttg cctttaaata atctatcaat
    12121 tagaataaac gtacagcgag ttaaggatag tgtaagctta acaataagat tggcgcaacg
    12181 aatcatttta aaataaaagc gagtgactac actaatttgg gtggaaccgc gggttaaata
    12241 cgtaacttat tttgtattat atacaattta aaatattaac tcgtcccatg gaagtaaagc
    12301 atatttacta tgctgttatt ttccatgggg cgttttcatg tatgaggaga ggtaattatg
    12361 gcaaaagata tggatacaat tgtttcatta gcaaaacaca gaggttttgt gttccctggt
    12421 agtgatattt acggtggttt atcaaacaca tgggattatg gtcctttagg tgttgaatta
    12481 aagaataatg ttaaaaaagc ttggtggcaa aaattcatta cacaatcacc gtttaacgtt
    12541 ggtatcgacg ctgcaatctt aatgaatcca aaagtatggg aagcttcagg acacttaaac
    12601 aacttcaacg acccaatgat tgataataaa gatagtaaaa ttcgatatcg cgctgataaa
    12661 ttaattgaag attatatgca agatgttaaa ggtgatgaaa acttcattgc cgatggttta
    12721 agttttgaac aaatgaaaaa aattattgac gatgaaggta ttgtttgtcc tgtaagtaaa
    12781 actgctaact ggactgaaat tcgccaattc aatttaatgt ttaaaacatt ccaaggtgta
    12841 actgaagatt ctacaaatga aattttctta cgtcctgaaa cagcacaagg tatttttgta
    12901 aactataaaa acgtgcaacg ttcaatgcgt aaaaaattac catttggtat cggtcaaatt
    12961 ggtaaatcat tccgtaatga aatcactcca ggtaacttca ttttcagaac aagagaattt
    13021 gaacaaatgg aacttgaatt cttctgtaaa cctggagaag aaatcgaatg gcaaaattat
    13081 tggaaaactt ttgcaagtga ctggttaaca agcttaaata tgagcagtga aaatatgcgt
    13141 ttacgtgatc atgatgaaga tgaattatct cattactcaa atgcaacaac tgatattgaa
    13201 tataaattcc catttggttg gggtgagtta tggggtatcg caagtcgtac agacttcgac
    13261 ttacgtaaac atgctgaaca ctctggtgaa gatttcagat accatgatcc agaaacgaat
    13321 gaaaaatata ttccatattg tatcgagcca tcacttggtg cagatcgtgt aacattagct
    13381 ttcttatgtg atgcatatga tgaagaaggc gttgaaggta gtaaagatgc acgtacagtt
    13441 ttacacttcc atcctgcatt agcaccatat aaagcagcga ttttaccttt aagtaagaaa
    13501 ttatctggcg aagcgattaa gatttttgag caattaagtt ctaaattctc aatcgatttc
    13561 gatgaatcac aatctatcgg taaaagatac cgtcgtcaag atgaaatcgg tacaccttat
    13621 tgtgtaacat tcgactttga ttcattagaa gataatcaag ttacagtacg tgacagagat
    13681 tcaatggaac aagttcgtat gccaatctca gagttagaag ctttcttaac tgaaaaaaca
    13741 aaattctaat taaaaagact ctccaccata tgtggtggct atataattta taaaagagat
    13801 actggttagt aaaattgcca gtatctcttt tctattcaaa aaggtattca aataaacaca
    13861 tctatgtggc tacttgaata ccttttatta ttttatgtta ttgttccaat ctttttaatt
    13921 ggttgattag tttctgactt ttaaaaaaca tacctgcata ttcacgatat aacattaaaa
    13981 tgatatctga catttcatca ataatttctt gatggatatt caatgaattc attttatcta
    14041 tcggtaattt ttgtaataca tctaataaat atagtgtttt attcgataat ataactgcat
    14101 gtacatcttt agaagcctct tgccttgaaa tcgcaccgtc aaacttaaaa ctataaccta
    14161 ttaaatctgc ttgtgtgtca ttaccactca cagcacagcg attaaatgat gcagtaaaac
    14221 caaatcgttt catgcacttt aacataacta cgactgacat taactgtgca gatgtacctg
    14281 attctatttt ttcaagaaca aattgtaata attgatagtt atatggtgca atgtcacctt
    14341 catccattga gcgctcaata gtttcagctg ccagagaggc ataactgctt acgtaaaggt
    14401 ccatttgtaa tttataatgt tgactaataa catctacaga atttaacgtt cccatacctc
    14461 gccactgatt gtaaataaac aaaccataaa caaacaattg cgtttgcgct tgtaaacccg
    14521 tcttaacttt tttagcacgc cttgccataa gtggtacttt tgcaccatgc tcatttaaaa
    14581 tcgtgataat tttatcagat tcaccataat caactgcttt gatgataatc cctttttggc
    14641 gcattaacaa ttatcttcac cacttttaag attaatcttg gtcttcaaca taaccaattt
    14701 ggcgaataaa gttaactttg tttcgccagt ctctttgaac tttgacccat aattctaagt
    14761 aaactttaga gcctagaagc atttctatat cacgtctcgc acgttttcct acttctttta
    14821 actttttacc gccttttcca atgacaattc ctttttgcga atctctttca acatatatag
    14881 ttgcttcgat atgaacacga tcttcgcttt ctttaaccat acggtccaca ttaacaccaa
    14941 tcgcatgagg gatttcttca cttgtaagat gaaggatttt ttcacgaatg atttcaccca
    15001 ctacaaattg ttcaggatgg tctgaaattt gatcatctgg ataatattta ggtccttcgg
    15061 gtaaatacgt ctttaaaaca tcaataaaat gatctacatt tagcccttct aatgctgaaa
    15121 taggtacaat ctctgtaaag tccatataac tttgatattc ttcaatcttt ggcattaatt
    15181 catctggatg cactaaatct attttattta atactaaaaa tactggtgtc ttaacatttt
    15241 tcaacatttc tataatatat tcatcacctc gtccaatttc ctcattggca ttaaccataa
    15301 acatgattgc atctatctca gataatgtat ttttagcgac tttcatcata tagtcaccta
    15361 atttgtgttt aggtttatga atacctggcg tatcaatgaa tataatttgc gcgtcatctc
    15421 ttgtcataac accttgaatt ttatttctag ttgtttgagc tttatcggac atgattgcta
    15481 ttttatggcc gatcactcta ttaacaaatg ttgacttccc tacatttggt ctacctataa
    15541 ttgaaacaaa tcctgattta tgttctgtca tttattctaa atcctttcct gaaaatccaa
    15601 atggtagtaa ctctgcgact gtcatcataa ccatatctcc tttatgattt gtcatataca
    15661 caggcatatc atcatcacat aattccttta aaacttgacg acatgcacca caaggtgatg
    15721 acggtttatc tgcatctacg gttacagtta ttgattcaaa atcacctggt ctgtatcctt
    15781 gagaaattgc cgataccaaa ctagctcgtt cagcacatat cgataatgga taagaagcat
    15841 tttctacatt ggtaccataa aaagttctac cgtctttcgt ttttaaataa gctcctactt
    15901 taaattgact gtatggcgaa tatgattctt gttgtgcttt tctaacttct tgaaaataat
    15961 gaggttgata actcatatat gcctccctaa aataacgcta taaaatgtgg taaaaatact
    16021 attaaaccta taataaatgc taatattgaa actataagta cactaaaagc cgctatatct
    16081 tttgcgtatt tagctaaatc atgatattca acggtcacta aatcgacaac atattcaata
    16141 gcagtgttta aagcttcaac agtgagaact aatgcaatag caatgagtat aaatatccac
    16201 tcaatacgat taatatttag tacgagacca aagacaatag caacaatcat tgcaaacaca
    16261 tgtaaaagaa atttatagtc tttttgaatt aagattttca gcccatcaag tgcatattta
    16321 aaccttttca taattagcct cgtgttaatc catatgcgtt taatattgta tcttgtcgac
    16381 caaacatttc cttttcatcc gcttcagtca tatgatcata acctaataga tgcaaaaatc
    16441 catgtaatgc taaaaatcct aattctcgtt caaaagaatg tccgtaattg tttgcttgtt
    16501 cttgcgctac atccgtacag ataattatat cccctaaaac acgtggtata tcaagaccac
    16561 taaaatcaat ctctggctca tcttcttcta aagcaaatga gattacatct gtaactttat
    16621 ctttatctct atatgttcga ttaatttctt gtatttcttg tttatctaca aatgtaacag
    16681 aaagctcagc atcgtcttct atatgctctt cttttttagc aaattctaat aaatcttcaa
    16741 tttgtttata ccacgcatct ttaactaagc ctgtgtgatc gctaaaatct atcgtaaaca
    16801 tttaattctc tccttcataa tgttcaatga tcttacttac caatggatgt cttactacat
    16861 cgctctgatc taatttcaat atacttatac ctttaacgtt gtgtaaccta ctgaccgctt
    16921 ccttaagtcc acttttaaca cctttaggta aatcgatttg agtttggtca ccagtaacta
    16981 ccatttttga gccaaaacct agtcttgtta aaaacatttt catttgcgca tgtgtcgtat
    17041 tctgcgcctc atcaagaatt acaaatgcat cttctaatgt tcgtccgcgc atatatgcaa
    17101 gtggcgctat ttcgataatg cctctttcaa taaatcgctc tgtttgttca cgcccaagaa
    17161 cagtatatag accatcatat aaaggtctta aatatggatc tatcttttct ttcaaatctc
    17221 ctggtaaaaa tccaagtgac tctcctgctt caacagcagg tcttgttaat acaatacgtt
    17281 taacagcacc tttacggagt tgctttgctg cataaactac agctaagaat gtcttacctg
    17341 taccagcagg acctatacca aatactaaat cattattttt catggcatta acatatatac
    17401 gttgccccat cgttttcgca cgaattgtct taccaaatgc atctttagtt atctcttcat
    17461 catataaatc taacagatgt tgaattgtgt tattatgcgc cattttaata gctgcttcaa
    17521 catctttaat tgtaatatta ttacctaaat caataacctt cagcaaattg attaatactg
    17581 attccgcttt ttctacgttt tctatttttg tacctttaac ggcaacttct tgtcctcttg
    17641 catggatgac aacatcgaaa ctctcttcaa ttgcttttaa atgttcatca ttatttccaa
    17701 ttaaagcttg agattggttc atatcgtcta tttgtataat tccaggcata cacgcgctcc
    17761 ttttcatata tcatttatat tcattctatg atgctttaat atctacttaa ttatatcatg
    17821 caattgattg atttaaacat ctttgcattg acttactcat ttttctttaa tttaaaataa
    17881 tcatttatac ttttcacctt gtcataaaag cagattcaat tttagactaa tcaatttttt
    17941 gttgtaattt aaataactaa tatttctcga aagattctct ttgtttttca atttttctta
    18001 acgtaaaaaa acaagcaact accattacga tagtgcttgt tttcaaaaaa ttttataatt
    18061 gttttggttt agctaaaatt tctgaccata tcataccatt gattacttca tctttatcaa
    18121 attggaacgc tgatttagtc aaatcttttt cgacattttg agaattaagc aactgcttta
    18181 atttcaaacg ttttgtacgt tcagataagt atttatcctc aataatatct ctagcacgtt
    18241 tttccatttt agcaatttgc ttttcttttt ctctatcaat gtcagtacga atcgttttaa
    18301 tatcatctct aagtgatttt tctaattgac gtctaatttc gtcagaatta tcttgtctag
    18361 aaggtctatg tttctgctcc accggttctg ctttaggcac tgtaattggt ttctctgtca
    18421 tcggttttgt ttctggttgt tttttaggtg ccataggttc tacaacaggt ttcgatttag
    18481 gctcttcctt tggaagttca tcgaataaag gtggtaacgt atcatcatat tttcgtttcg
    18541 atgatttctt ttcttcttca tttaattctt cacttatttc tttaaacgtt cgctcaattt
    18601 cttcaaaaaa gccacctttt tttggttcat tatcggtaga tgttttttga ggtggctttt
    18661 gattttgtct atctttatga ctattttcgc gcatagtagt aatgatagaa atgatcactg
    18721 atatgacaaa aattagaata ccgacactca tttaatcacc tctcgactta atgttcaggt
    18781 gactcatcat cactttgatc agttcgttta ttaattgcat ttctcatgcc tgtatcggct
    18841 tcgatatttt tcaaattata ataatcttta acactgatat tacctgaacg taatgcttca
    18901 gccatagcta atggtacttc agattcggct tcaactactt tagcatgcat ttcttgtaca
    18961 cgcgctttca tttcttgctc agttgctaca gccatagctc tacgttcttc agcttttgct
    19021 tgtgcaatat ttttgtctgc taatgcttgt tcagtttgta agtctgcacc aatattttta
    19081 ctaatatcaa cgtcagcaat atcaattgat aaaatttcaa atgcagtacc tgaatctaaa
    19141 cctttgctta aaactgtttt agaaatatta tctgggtttt caagtacttc tgtatgatgc
    19201 ttactagaac caattgttga aacgatacct tcaccaacac gtgcgatgat tgtttcttca
    19261 cctgcaccac caacaagtcg agcaatatta gctctaactg tgatacgagc tttggctttc
    19321 acttcaatac cgttcattgc tacacctgcg ataaatggtg tttcaatgac tttaggatta
    19381 acagacattt gaaccgcttc taatacgtca cgtcctgcaa ggtcaattgc agcagcacgt
    19441 tcgaaaggaa gatcaatgtc agcacgttgt gcagcaatat tagcgtcaac aactctgtca
    19501 acatttcctc ctgctagata atgcgattct aattggtttg ttgttaatgc tagtcctgct
    19561 ttgtgcgctt taattaatgg cgctataact tttcttggag atacacgacg taaacgcata
    19621 ccaaccaatg tacctatacc aacatgaacg ccagctgcta acgctgaaat ccacaaacca
    19681 atcggtacaa atgagaataa aataagtaat gcaactatta taataactgc tattacgata
    19741 aaacttaaac taaacatggt atcgctcctt ttaattaatc tacttccctc acaactactc
    19801 ttgttccttc aacttcaagg attttcaccg ttttattgcg taaaataaag ttgccatctg
    19861 aaacagcatc aatacgttca ttttcacaaa aaataatccc tgcaggtcga agatctgtaa
    19921 ctgtttgagc agtctttcct acgaggtgcg agcggttatc atgagaattg taacctgact
    19981 cagaattagt tgaatctttt aagataactt tatccaaaaa cggaatcttt ctgttgaaaa
    20041 tcttcactaa tatcacccat tctacaatcg ttaaaatcaa ggcaacgata acattcgcaa
    20101 gcataaatag caaattatca ccgagcgttg ttatgcttat agttatcagt atcataccaa
    20161 taataccaat tactgcacca actacaaata attcaattac aactaatata acgccaattg
    20221 agaatattaa gatagaatgc atattgacat ttccttggat tagaaatccc aaaaataaaa
    20281 taagtaatga taatgtggcg ataataccag ctgcattgat ttttttagag taaagttgat
    20341 atacaaatcc taagaatgtc aaacaggtta atattaacgt aaaaataggt tgaacaatta
    20401 tattactaac ttgttcaacc caagtatctc cagccgttga ttccaagata gttgtcattt
    20461 gtaaaaaatt attataactc acaatttcac ctcgccctca cattaattat acatgaaatt
    20521 gaatatactt tata
//
LOCUS       DAHHYJ010000033        20063 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000033 DAHHYJ010000000
VERSION     DAHHYJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20063
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.033"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..276)
                     /gene="sarT"
                     /locus_tag="NBK22_000922"
     CDS             complement(<1..276)
                     /gene="sarT"
                     /locus_tag="NBK22_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarT"
                     /protein_id="HCD2414125.1"
                     /translation="MNDLKSKSNIKLMKRVLTTYELRKYLKKYFCLTLDNYLVLAYLD
                     VFKNDEGKYFMRDIISYIGIDQSRIVKSVKELSKKGYLNKCRDPHDSR"
     gene            600..1343
                     /gene="sarU"
                     /locus_tag="NBK22_000923"
     CDS             600..1343
                     /gene="sarU"
                     /locus_tag="NBK22_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCD2414126.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFL
                     DLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSLADERIVL
                     IKINKIQYNTIKSIFTDTSKILKPRKFFF"
     gene            complement(1554..2420)
                     /gene="galU"
                     /locus_tag="NBK22_000924"
     CDS             complement(1554..2420)
                     /gene="galU"
                     /locus_tag="NBK22_000924"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCD2414127.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
     gene            complement(2600..5482)
                     /gene="fnbB"
                     /locus_tag="NBK22_000925"
     CDS             complement(2600..5482)
                     /gene="fnbB"
                     /locus_tag="NBK22_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbB"
                     /protein_id="HCD2414128.1"
                     /translation="MKSNLRYGIRKHKLGAASVFLGTMIVVGMGQEKEASASEQNNTT
                     VEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAP
                     KVETSRVDLPSEKVADKETTGTQVDTAQPSNVSEIKPRMKRSADVAEVAEKEVVGEAK
                     TTGTDVTSKVEVTESSLEGHNKDSNIVNPHNAERVTLKYKWKFGEGIKKGDYFDFTLS
                     NNVETHGISTLRKVPEIKSTDGQVMATGQVIDERKIRYTFTDYINSKKDLTAELNLNL
                     FIDPTTVTKQGKQNVKVTLGNKEIEKEFDIKYLDGVKDRMGVTVNGRIDTLNKGDGKF
                     SHFAYVKPNNQSLTSVTVTGQVTKGSKSGASNPTVKVYKHNGSDELAESVYAKLDDAN
                     KFEDVTNKVNLSYTGDGGYSLDFSDLDNSKDYVIKYEGEYDQNAKDLNFRTHLSGYHK
                     NYPYYPNYYYPVQLTWNNGVAFYSNNAQGDGKDKPKEPIIEHSTPIELEFKSEPPVEK
                     HELTGTIEESNDSKPIDFEYHTAVEGAEGHAEGIIETEEDSIHVDFEESTHENSKHHA
                     DVVEYEEDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTVEDTKEYTTESNLIE
                     LVDELPEEHGQAQGPIEEITENYQHISHSGLGTENGHGNYGVIEEIEENSHVDIKSEL
                     GYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIQGQNNGDQSFEEDTEED
                     KPKYEQGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHSSVDFEEDTLP
                     QVSGHNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEV
                     PSEPEVPTPPTPEIPSEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPT
                     KKAQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA"
     gene            complement(5466..5568)
                     /locus_tag="NBK22_000926"
                     /pseudo
     CDS             complement(5466..5568)
                     /locus_tag="NBK22_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6161..9214)
                     /gene="fnbA"
                     /locus_tag="NBK22_000927"
     CDS             complement(6161..9214)
                     /gene="fnbA"
                     /locus_tag="NBK22_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCD2414129.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATDNKVNETQTTTTNVNTIDETQSYSATATEQPSNATQVTTEKAPKAVQAP
                     QTAQPANVETVKEEVVKEEANPQVKETTQSQDNSGDQRQVDLTPKKATQNQAAETQVE
                     VAQPRTVSESKPRVTRSADVVEAKEASNASEIKGTDVTSKVTVEDESKIEAPKGNNVQ
                     PHEGQRVVLKYKLKFQDGLKTGDYFDFTLSNNVNTHGVSTIRKVPDIKNGSLVMAKGQ
                     VLDNGRIRYTFIDYIKDKVNVTANLEINLFIDPKTVQSNGQQTITSKLNGKETSGTMQ
                     ITYKDGVKNQYTNVNGSIETFDKEKNKFTHVAYIKPINGNNSDSVTVTGMLTQGSNEN
                     GTQPNVKIYEYVGTENGLPQSVYANTVDSTQLKDVTNQMSDKLKVQNNGSYSLNFDKL
                     DKTYVIHYTGDYLNGTSEVNFRTQLTGYPENHYKTYYYYNHGYTLTWDNGLVLYSNKA
                     NGDGKYGPIVDSNNFEFSEDSGNGSISGQYDAKQIIETEENQDNTPLDIDYHTAIDGE
                     GGYVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSK
                     HHADVVEYEEDTNPGGGQVTTESNLVEFDEESTRGIVTGAVSDHTTVEDTKEYTTESN
                     LIELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIK
                     SELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGDQSFEEDT
                     EKDKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNIVDFEED
                     TLPKVSGQNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVP
                     SEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTK
                     KAQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNNKA"
     gene            complement(9198..9300)
                     /locus_tag="NBK22_000928"
                     /pseudo
     CDS             complement(9198..9300)
                     /locus_tag="NBK22_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9683..11041)
                     /locus_tag="NBK22_000929"
     CDS             complement(9683..11041)
                     /locus_tag="NBK22_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCD2414130.1"
                     /translation="MFNEIWPLISVILGIVILLILIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGLIFILFISL
                     FV"
     gene            complement(11161..12711)
                     /gene="gntK"
                     /locus_tag="NBK22_000930"
     CDS             complement(11161..12711)
                     /gene="gntK"
                     /locus_tag="NBK22_000930"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCD2414131.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDENGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWAD
                     NRAAKYATVINEVHYGNAIYQRTGTPIHPMSPLAKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISEEMLPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSELWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHTPIKENVAVYQELVSIFINLSRSLTENYEKIADFQR
                     QHMAENKT"
     gene            complement(12735..13415)
                     /locus_tag="NBK22_000931"
     CDS             complement(12735..13415)
                     /locus_tag="NBK22_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2414132.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(13569..14333)
                     /locus_tag="NBK22_000932"
     CDS             complement(13569..14333)
                     /locus_tag="NBK22_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD2414133.1"
                     /translation="MSNYSIGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNEASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLITLPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISHIQHNN"
     gene            complement(14498..15190)
                     /locus_tag="NBK22_000933"
     CDS             complement(14498..15190)
                     /locus_tag="NBK22_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD2414134.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(15421..15837)
                     /locus_tag="NBK22_000934"
     CDS             complement(15421..15837)
                     /locus_tag="NBK22_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCD2414135.1"
                     /translation="MPFTMKGYPEKWKEFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(15976..17799)
                     /locus_tag="NBK22_000935"
     CDS             complement(15976..17799)
                     /locus_tag="NBK22_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD2414136.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDSFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLAIFENIAK
                     HFELNTELEIKILPDLPQYNYIQSAMMNIQHNGFRERDKNV"
     gene            complement(18277..19554)
                     /locus_tag="NBK22_000936"
     CDS             complement(18277..19554)
                     /locus_tag="NBK22_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2414137.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
BASE COUNT         5908 a         3821 c         2940 g         7394 t
ORIGIN      
        1 tctactatca tgtgggtctc tacatttatt caagtaaccc ttttttgata attcttttac
       61 gcttttaaca attctagatt ggtctatacc gatataactt ataatgtctc tcataaaata
      121 tttaccttca tcatttttaa atacatctaa atatgctaat actaaataat tatctaatgt
      181 aagacaaaaa tattttttta aatatttacg cagctcatat gtagttaata ctcttttcat
      241 taatttaata ttgctcttgc ttttcaaatc attcatcaag tcttcatccc ttacttttaa
      301 ttgtcacgtc aaataatatt tgtctttaac tagtaataac tccaaatata tttagtttgt
      361 ggtgctagct ataaacacaa atgaatcata acactatatt ttgattttgt tcaattgttt
      421 ttgtttttgg ataataaatg aatttgtcat ttgttattcc gtgattttta gttatttata
      481 ttttatatat attatatata aaaatattta ggttaacatg tcttcaaaag agattgcttt
      541 tatattactt taaataatat aaagtattgg gacatcagaa aatagtagga gaatgtaagg
      601 tggactatca aactttcgaa aaggtcaata aattcataaa tgtagaagcg tacatatttt
      661 ttctcactca agagttaaag caacaatata aattatcatt aaaagaattg ttgatattag
      721 catattttta ttacaaaaat gaacacagta tttcactaaa agaaatcatt ggtgacatac
      781 tttacaaaca atctgatgtt gttaagaaca ttaagtcact atctaaaaaa ggatttataa
      841 ataagtctag aaacgaagca gatgaacgcc gtatttttgt ttcagttact ccaatacaac
      901 gtaaaaagat tgcttgtgtt attaatgagt tagataaaat aattaaagga tttaataagg
      961 aaagagacta cataaaatat caatgggctc caaaatatag caaagaattt tttatacttt
     1021 ttatgaacat tatgtactca aaagattttt taaaatatcg atttaattta acatttcttg
     1081 atttatctat cttatatgta atatcatctc gaaaaaatga gatactaaat ttaaaagatt
     1141 tgtttgaaag tattagattt atgtatcctc aaattgttag gtcagttaat agattaaata
     1201 ataaaggtat gctaatcaaa gaacgatccc ttgcagatga aaggattgtg ttaatcaaaa
     1261 taaataaaat acaatataac actattaaaa gcatattcac agatacttcc aagattctca
     1321 aaccaagaaa atttttcttt taaatttaaa cagatttacc tcttgataaa ataaataagc
     1381 aatcataata cttctcaatt tagtataaat aaaaatacat aattaacttt cttttgtttt
     1441 tatattattt caatacccta ctatatatca caacacataa attaagcatg acactcattc
     1501 aatttagttc accatttcgt gttccaattt tattgagtat catgctttta atgttataac
     1561 cctaattctt taataaatcg tgttaattct tctcgcatac tgtcatcttt caatgcatat
     1621 tctatggtag ttttaacgaa gcctaatttt tctccaacgt cataacgttc gccttcgaag
     1681 tcatatgcat acacttggtt atcattattc atacgttcaa tcgcatctgt taactgaatt
     1741 tcgttacctg cgccttcttt ttgcgttttt aaataatcga aaatttcagg cgttaataca
     1801 taacgtccca taatagctag gtttgatggt gccgtacctt gtgctggctt ttccacaaac
     1861 tttttcactt catactgacg tccgttttta gttaatggat caataattcc ataacgatga
     1921 gtatctgctt ctggaacttc ttggacgcct ataactgagt gcccagtttc ttcataaacg
     1981 tcaatcaact gtttcactgc tggtacttca gattcaacaa tatcgtcacc taataacacc
     2041 gcaaatggtt cattgccgat aaattgacgc gcagaactaa tcgcatgccc caaacccttt
     2101 tgttctttct gtcttacata aaaaatattc gcaagttccg ttgaatactg aactttttct
     2161 agtaattcag atttaccttt ttcttttaac accatttcta attctttttg actatcaaaa
     2221 tgatcttcaa tcgcgcgttt gtggcgacct gtcactataa taatatcttc aattccagct
     2281 cttgcagctt cttcaacgat atattgtatt gtgggtttat ctaagatagg aagcatttcc
     2341 tttggcatcg ctttagttgc tggtaaaaat ctagtcccta aaccagcagc gggaatgatt
     2401 gcctttttta tttttttcaa agttaatgtg ctccttttcc taagtattaa atctatgtat
     2461 caacgtcatt ttaacactaa ttagaacgcc ttcatagtgt cattgagtat gtaattattt
     2521 cttgggaaat ttgatttaat tttaaaaaac aggcttactt catataattt atgaaataaa
     2581 cctgccaatt ttggattgat tatgctttgt gattcttttt atttctacgt aataacgcta
     2641 atcctaaaat gctaaataat ccgccgaaca acataccttt gtttgttgat tcttctccac
     2701 ctgtttcagg tagttcagat ttcttagatt gtgctttttt agttggtgcc actgctttaa
     2761 ccttttcatt gatttcaata acaggtgtta ctaccttacc ttgttccact ggtttagaag
     2821 gctttttagg ttcttctttt gcgggtggta ctggtttacc tggctcagat ggtatttctg
     2881 gcgttggtgg cgttggtact tccggttcac ttggtacttc tggtgtcggt ggcgttggtg
     2941 tttccggctc actcggcact tccggcgtcg gtggtgttgg tgtttccggc tcgcttggta
     3001 cttctggtgt cggtggcgtt ggtggcacga ttggaggtgt tgtatcttct tcaatcgttt
     3061 gttgaccttc attatgacca cttacttgtg gaagtgtatc ttcttcaaag tcaacactac
     3121 tgtgtccacc gaattgataa ttaggtttat ctttgtttgt atcttcttca ataatttcag
     3181 tgtgcttatt gaatccatga atatgtggca cactatcgaa gtcgatatca atgatattac
     3241 caccttgttc atacttaggt ttgtcttctt ctgtgtcttc ctcgaatgac tggtcaccat
     3301 tattttgacc ttggatttga ggtacactat cgaaatcgat atctacgata ttgccacctt
     3361 gttcatattt cggtttatct tcttctgtgt cttcctcgaa tgactgatta ccgctatttt
     3421 ggccaccttc gtaacctaat tcactcttaa tatcaacatg gctattttct tcgatttctt
     3481 caatcacgcc ataattaccg tgaccatttt cagttcctaa accagaatga gaaatatgct
     3541 ggtaattttc agtaatctct tcgattggac cttgagcttg accatgttct tcaggtaatt
     3601 catccactag ttcgataaga ttactttcag ttgtatattc tttcgtatct tcaactgttg
     3661 tatgatcgct cactgcgcca gttacaatac cttttgtaga atcttcgtca aattcaacta
     3721 ggttagactc agtagtaacc tgaccaccac ctgggtttgt atcctcttca tattcaacaa
     3781 catcagcgtg atgttttgaa ttttcatgtg tagattcttc aaaatccaca tgaatagaat
     3841 cttcttcagt ttcaataata ccttctgcat gaccttctgc accttcaaca gctgtatgat
     3901 attcaaaatc aattggctta gaatcgttac tttcttcgat tgtaccagtc aattcatgct
     3961 tctccactgg cggctctgat ttaaattcaa gttcgatagg agtactatgt tctataatag
     4021 gttcctttgg tttatctttg ccgtcgcctt gagcgttatt agagtaaaat gcaacgccat
     4081 tgttccaagt tagttgaact ggataataat agttaggata gtatgggtaa tttttgtgat
     4141 atcctgaaag atgtgttcgg aaatttaaat ctttagcatt ttgatcatat tcaccttcat
     4201 atttaattac atagtctttc gaattatcta aatcgctaaa gtccaaagaa taaccgccat
     4261 ctcctgtgta acttaagttt actttgttag tcacatcttc aaatttattg gcatcatcaa
     4321 gctttgcata aacactttca gctaattcat ccgaaccatt gtgtttatat actttgactg
     4381 ttggattgct agcccctgat ttacttcctt tagttacttg accagttact gtcacagaag
     4441 ttaacgactg gttgttaggc ttcacgtatg caaaatggct aaatttaccg tctcctttat
     4501 tcaaagtatc aattcgacca ttaacagtta cacccattct atctttaaca ccgtctaagt
     4561 atttaatatc gaactctttt tcaatctctt tattacctag tgttacttta acattttgct
     4621 ttccttgctt tgttactgtt gttgggtcaa tgaataggtt taagtttaat tcagcagtta
     4681 aatctttttt gctatttata taatcagtaa atgtataacg aattttacgt tcatctataa
     4741 cttgacctgt tgccataact tgaccatctg tactttttat ctctggaact ttacgtagtg
     4801 ttgagatacc atgagtttct acattattac ttaacgtgaa atcaaaataa tctcctttct
     4861 taattccttc accgaatttc catttatatt ttaaagttac tctttcagcg ttatgaggat
     4921 taacaatatt cgaatcttta ttatgtcctt ctaaagagct ttcagtaact tccactttac
     4981 ttgttacatc tgtacctgtc gtcttagctt ctcctactac ttctttctct gccacttctg
     5041 caacgtcagc tgatcttttc attcttggtt taatttctga gacgttactt ggttgagctg
     5101 tgtcaacttg agttcctgta gtttccttat cagcaacttt ttccgatggc aaatcaactc
     5161 gcgaagtttc tacttttggt gcttgcacag ttttcggtgc ttcttctgtt gttacttgtg
     5221 ttgattgtga tggttgctca gttgatgtcg cgctatatga ttgtgtttca tctattgtat
     5281 taacgttatt tgtagttgtt tgtgtttcgc ttgctttact ttcagtagct gaactcccac
     5341 tttcctctac tgtagtattg ttttgttccg atgctgaagc ttctttttct tgtcccattc
     5401 caacaacgat cattgttcct aagaatactg aggccgctcc caatttgtgt tttcttatgc
     5461 cgtatctaag attgcttttc actataatat tctcccttaa atgtaaaatt catttatttt
     5521 tttaaactca ataaatgcaa ttctatattg ttcggttttt aaaagcaatg aaaaaaagcg
     5581 agttaataaa aagttaagat tgttattaac tttatgtata atgagttttt tattatttgg
     5641 aactcacata tatattgcac acaaaactct tgaatacctt gatataactg gcttgtattt
     5701 tttatactta ctttttaaat taaattcaaa ttcatctgat ttaaaacaat atactaaaac
     5761 atacataata atcgcctgta caatgcatca ttagcaagtc actgaaacgc ctttcattgt
     5821 attaataacg tcactataat ttttatatcg ttcggttttt gtttggtttt aatgattatt
     5881 tatacagaaa cagccgtatt tcaagacgac attttaaatt taaccaaatt tgcatctagt
     5941 taataattgc atttatcaaa tttgtcttat tgatccaatc ttatttgtac ttgcaaacta
     6001 gttttaaatt ctaactttat ctctcagttc gttatccatc agacataaac caatgaagca
     6061 atcagaaaac actctaattt tctattagaa atttgattta atataaaaaa acaggcttac
     6121 ttcatataat ttatgaaata aacccgtcaa tttttgttaa ttatgctttg ttattctttt
     6181 tatttctacg taataacgcc aaacctaaaa tgctgaataa tccgccgaac aacatacctt
     6241 tgtttgttga ttcttcccca cctgtttcag gtagttcaga tttcttagat tgtgcttttt
     6301 tagttggtgc cactgcttta accttttcat tgatttcaat gacaggtgtt actaccttac
     6361 cttgttccac tggtttagaa ggctttttag gttcttcttt ggcaggtggt actggtttac
     6421 caggttcagc tggcacctct ggcgttggtg gtgttggtgt ttccggctca cttggtacct
     6481 ctggtgttgg cggtgttggt gtttccggct cacttggcac ttctggtgtc ggtggtgttg
     6541 gtgtttccgg ctcacttggt acttctggtg tcggtggtgt cggcggcgtt gtatcttctt
     6601 caatcgtttg ttgaccttca ttttggccgc ttacttttgg aagtgtatct tcttcaaaat
     6661 caacaatgtt gtgtccaccg aattgataat taggtttgtc tttatttgta tcttcttcaa
     6721 taatttcagt gtgcttattg aatccgtgaa tatgtggcac gctgtcgaag tcgatatcaa
     6781 tgatgttacc gccatgttca tatttaggct tgtctttctc tgtatcttct tcgaatgact
     6841 gatcaccttt attttgacca tgaatttgag gtacactatc gaaatcaata tctacgatat
     6901 tgccaccttg ttcatattta ggtttatctt cttctgtgtc ttcctcgaat gactggttac
     6961 cgctattttg gccaccttca taacctaatt cactcttaat atcaacatga ctattttctt
     7021 caatttcttc aatcacgcca taattaccgt gaccattttc agttcctaaa ccagaatgag
     7081 aaatatgatg attgttttca gtgatttcct cgattggtcc ttgtgcttga ccatgttctt
     7141 caggtagttc atccactagt tcaatcagat tactttcagt tgtatattct ttcgtgtctt
     7201 caactgttgt atggtcgctc actgcgccag ttacaatacc tcttgtagac tcttcgtcaa
     7261 attcaactaa gttagactca gtagtaacct gaccaccacc tgggtttgta tcctcttcat
     7321 attcaacaac atcagcgtga tgttttgaat tttcatgtgt agattcttca aagtcaattg
     7381 gatttgattc ctcagaggac tcagtgtatc ctccaacgtg acccgcttcg ctatccacag
     7441 cagtatggta atcgatatca atagctgatg aatccgtttc ttctattgtt tcaatgtatc
     7501 catcaacata accaccttca ccatctatag ctgtgtggta atcaatgtca agcggtgtat
     7561 tgtcttgatt ttcttctgtt tcaataattt gcttcgcatc gtattgtcca ctgatagaac
     7621 cattaccact atcttcagag aattcgaagt tattggagtc tacgattgga ccatattttc
     7681 catcaccatt agctttatta ctatataaaa ctaatccatt atcccatgtt aatgtatagc
     7741 cgtggttata ataataatat gttttatagt gattttcagg gtatccagtt aattgagttc
     7801 taaagttcac ttcactcgta ccatttaagt aatctccggt gtaatgaatt acatatgttt
     7861 tatctaattt gtcaaaattt aatgagtagc taccattgtt ttgtactttt aatttatcac
     7921 tcatttgatt cgttacatct tttaattgtg tgctgtccac tgtatttgca taaacacttt
     7981 gtggtagccc attttcagtt ccaacatact catatatttt aacatttggt tgtgttccat
     8041 tctcattact tccttgtgtc aacatacccg ttacagtaac actgtctgaa ttgtttccat
     8101 ttataggctt aatatatgct acgtgtgtaa atttattttt ttctttgtca aacgtttcaa
     8161 tcgaaccatt tacatttgta tattgatttt taacaccatc tttatatgtt atttgcatag
     8221 ttcctgatgt ttctttgcca tttaattttg aagtaattgt ttgttgtcca ttgctttgaa
     8281 ctgttttagg atctatgaat aagtttattt ctaaattagc cgtaacattc actttatctt
     8341 tgatgtaatc aataaacgta tatctaattc ggccattatc tagtacctgt cctttagcca
     8401 ttactaatga accattttta atatctggaa cttttcttat agttgatact ccatgcgtgt
     8461 ttacattatt tgataatgta aaatcaaaat aatctcctgt tttcaaccca tcttgaaatt
     8521 tcagtttata cttcaatact acacgttgcc cttcatgagg ctgaacatta tttccttttg
     8581 gcgcttcaat tttactttca tcttctactg taactttact tgtcacatct gtacctttaa
     8641 tttctgaagc gttactagct tccttagctt caactacgtc tgctgatctt gtcacacgtg
     8701 gtttactttc tgataccgtt cttggctgtg ccacttcaac ttgtgtttct gctgcttgat
     8761 tttgtgtagc ctttttaggt gttaaatcta cttgtctttg atctccgcta ttgtcttgag
     8821 attgtgtcgt ttccttaact tgagggttcg cttcttcctt aactacctct tctttaactg
     8881 tttctacatt tgctggttgt gcagtttgtg gtgcttgtac tgcttttggt gctttttcag
     8941 ttgttacttg tgttgcgttt gacggttgtt ctgttgctgt tgcgctgtat gattgtgttt
     9001 catctatagt attaacgtta gttgtagttg tttgtgtttc gtttacttta ttatcagtag
     9061 ctgaattccc attttcttct actgtagttg tcttttgttc tgatgctgca gcttctttat
     9121 cttgtcccat cccaacaacg atcattgttc ctaagaatac tgatgctgct cccaatttat
     9181 gttttctaat gccgtaccta agattgtttt tcactataat atctcccttt aaatgcaaaa
     9241 ttcattaatt tttttaaact taataaatgc aagtctatat tgttcggttt ttaaaagcaa
     9301 tacaaaaaag cgagttaatg aaaagttaag attgtcatta attttatgtt aattgataat
     9361 tttattattt gaaatatacc tataaattgt attcaagtca tcagaaaccc ttgtcacaca
     9421 aggcttgtat tttttatact tattttttaa attaaattca tcattatcta atttaaaaca
     9481 atatactaaa agtttcataa ttatcgcctg tacaatacgc acaaaaacac gtcttgaaac
     9541 gcctttcatt acactaaaat acccaatata ctttttatat cgttcggatt ctgagtattt
     9601 cagacgattt tctgcataaa aataaacgtg tttcaaggca atatattgta attacctaaa
     9661 aacacgttta cttaatattt agttaaacaa ataagctgat gaataaaatg aagatgagac
     9721 ctgaaacgga aataatcgtt tctaataatg accatgttaa gaatgtttct tttacagtta
     9781 aaccaaaata ttctttaaac atccaaaatc ctgcgtcatt tacatgagac aaaatcacac
     9841 tacctgcacc tatcgcaagt acaactaatg ctacatttac atctgatgat tgtaataatg
     9901 gtaagacaat acctgtagtt gaaatcgcag ctactgtagc cgaacctaac gcgatacgta
     9961 gcacagctgc aacaatccat gctagtaaaa tcggcgacat ctctgtacct tcaaacattt
    10021 tagcaattgt atttccgaca ccaccgtcaa ttaatacttg tttaaatgta ccgccaccgc
    10081 caataatcaa tagcatcatt ccgattggat aaatcgcatt cgtcactgat tccataatat
    10141 gattcatctt acgttttctc attaatccca tggtaacgat tgcaaataat actgctatta
    10201 gcatggctgt ccctgctgtt ccgatcatat aaatgataga ttcaaataga tttgtaggtt
    10261 tgtcgtgccc ggttacaagt tgcgttatcg tagatactaa cattaatatg actggtaatg
    10321 ttgctgttaa taaactcata ccaaatcctg gcatctcttg atccgtaaat tctttttgtg
    10381 cacctaacgc tgaaatatcg ccttctcgtg tatacgcaga cggaatcatt ttttgtgcaa
    10441 ctttgttaaa tataggtcct gcaatgagtg taactggaat ggcaataatc ataccgtata
    10501 gtaatacatc tcctacattc gcctttaatt cttttgcgat gactaccggt cctggatgtg
    10561 gtggtaaaaa gccatgtgtc actgataaag ctgttaccat aggtagtcct agttttaaca
    10621 ctgaaacatt tgcgcgtttt gctactgtaa atactaatgg aatcagtaag actaaaccta
    10681 cttcaaagaa caatgcaatc ccgacgataa atgctgcaac aagcattgcc cattgtacat
    10741 gtttttgacc aaatttttga atcaacgtgt ctgcaattcg agttgcacca ccaccatcag
    10801 caagtaattt cccaagtatg gcacctaaac cgaatatcag tgcaatgtgg ccgagcgtac
    10861 tgcccattcc tttctcaatc gtctccataa ttttagtcaa tggtatacct agcattaacg
    10921 ctgtaatcat cgatgtgata attaatgaaa taaatgtatt taatttaaac ccaataatta
    10981 atattaataa aataacgata cctaaaataa cactgattaa cggccatatt tcgttaaaca
    11041 tgacattccc ctctttctct tttcaataga atgtaacacc gtcgtcgcgc acgtattcca
    11101 cataatatat agtagtcatg gcacaacaac tttatctaag atgcttgagt gacgtattta
    11161 ttatgtttta ttttcagcca tatgttggcg ttgaaaatct gcaatttttt cataattctc
    11221 tgttaaagaa cgacttaaat tgataaaaat ggatacgagc tcttgataaa cagcgacatt
    11281 ttctttaatc ggcgtatgat tgtttgtggc accgaccatc gatgaaacga ttgaaaaatc
    11341 ttcaatgtca cctacagctt taagcccgag tacgcaggca cctaagcatg aactttcata
    11401 actttcagga accactaact ctgtgtcaaa tatatctgac atcatttgac gccataattc
    11461 acttttcgca aaaccacctg ttgcttttat catcttaggt gtttcattca ttacttcaat
    11521 aagcgcaaga tagacggtat acaaattgta aagaacacct tctaatgcag cgcgaatcat
    11581 atgttctttt ttatgagata aagttaaacc gaagaatgaa cctcttgcat ttgcgttcca
    11641 aagcggtgca cgttctcctg ctaaataggg atgaaatatt aagccatcag cacctggttt
    11701 aacacgcttt gcaatttgag ttaaaacatc ataaggatca acaccgagac gtttcgcagt
    11761 ttcgacttcg cttgctagca actcatcgcg caaccatctc aatacgacac cgccattatt
    11821 tacagggcct ccaatgacgt agtggtcctc tgttaagaca taacaaaata ttctaccttt
    11881 gtaatcagta cgtggtttat ctatcacagt acgaatcgcc ccagatgtac cgattgtgac
    11941 agcaacttct cctttaccaa cactattgac acccaaatta gaaaggaccc catcactcgc
    12001 accaataaca aacggtgtat cttcatttag ccccattaat gttgcataac gttctttcat
    12061 acctttcatc acatacgttg ttggcactaa ttccggcaac atttcctcgg aaataccaag
    12121 caattctaat gccccaacat cccaatctaa tgtttctaaa ttaaacatac ctgttgcgga
    12181 agccattgaa taatcaatga tatatgtatc aaataaatga tagaaaatgt atgttttaat
    12241 atctgcaaac ttagcagtac gttgaaatac atcttgccat tcatgtttca tccaaaaaat
    12301 cttcgctaat ggcgacatag gatgaatagg cgtacctgtt cgctggtaaa tcgcgttgcc
    12361 ataatgcact tcatttatta ctgttgcata ttttgcagcg cggttatctg cccaagtaat
    12421 attatttgtt aatctttgat gttgctgatc catcgcaatc aagctatgca tttgagcact
    12481 aaatgacaca aacttaatat catcttgatt aactttcgat tctctcatta tgtatttaat
    12541 tgtcattaat accgcatcaa ataattcatc tgggttttct tctgagacat caacgtttgg
    12601 tgtgtgtaaa tcataaccga tttgatgttt catgataaaa gttccatttt catcatataa
    12661 gactgacttg gtactcgtcg ttccaatgtc gacaccaatc atatatttca tgataaatcc
    12721 ttctttcttt cattttaatt caaccaaaat ccttcaatat ctttaccaac atcgtcgaaa
    12781 tttaaatgaa acgcttcttt caaaatttga ctgtcgtatt gttccactgc atcaataaac
    12841 acttgatgat tatgatgtat gcgttcaaaa tcttgcgggt tctgttgcat acgttgtcgc
    12901 atggatgtta aaacgagtgc catcattact ggttttaaat gactccaaaa tgagtttaaa
    12961 tattgatgat tagatgcttt gattaatgtt tcatgaaatt caaagtcatg cttcgtaaat
    13021 gattctgcat cctcaaattt tactgccact ttcatcattt caagttgttt cttcatttct
    13081 tttacgatag gtagtcgctc ttgattttta actcttgaaa atgcaaatga ctctaacatc
    13141 agtcgcaaat catacatttc tttcttttct tgttccccaa acggcaacac atgtgcaccc
    13201 attctttcta attggatgag ttgattttgt tgcaataatt taaatgcatc tcgaattggc
    13261 gaacgactca cattaaattg ctttgccatt tgattttcag tgagtaacgt accttcagct
    13321 atgtgaccat tcacaatgcc taagcgtaat tctgccgcga taccttctcc agttgtcata
    13381 ccttccaacc atttctctgg atatccatac atcatcaaag tcactccttc attacacgac
    13441 atacttgtat acaagtatgt taatatagtt attatgagtt tgcaagcgct ttctttacga
    13501 gcactaaaat agtgaccacc ccttttcgat ttaaatttaa aggaaatggt cactatcaca
    13561 cgaatgattt aattgttatg ttgtatgtgg gatatttcta attgttctgt actcatatgc
    13621 gctttaggta cttcaatgca ataatgcgtt tcatgacagt ttggacattc gaatcgacgt
    13681 gttgtcgctg tatgtttcgc tttgataact gcccacaaag atggtgagaa tatatgctgg
    13741 cagttaggac ataaataggc aaccttttgt tggtaataaa aagtaacacc aatgccataa
    13801 ccaatcataa atggtaaagt aattaaaaac ggccatttat ttttcatcaa aattgcactt
    13861 ataatgctag aatattgaat tattcctata ataccagcac taatccaaat gttacgacga
    13921 atacttttca tttcagctga tttactcatg acatgctcta tgtcttttaa gtgtgtgatt
    13981 ggagacgtcg acgcttcatt tacgtaatat tgaacatttt taattttgtt taataccgct
    14041 tgttgctgtt taacttgttg gttaatttct tgttgtttca tagttagtaa agtattgagc
    14101 gtcttcaaag taccttcacc ttttagcaac atatctatat cgcttaacgc acaacctaaa
    14161 tctttaagca ataagattaa ctctaatgtt tgtcgctgtt gttctgtata cacacgacgc
    14221 tttccttctg taaatccttg tggtttcaaa atacctttgc gatcataata ttgaatcgtt
    14281 cgtgttgtca cattgcataa ttttgcgagt tctccaatcg aatagttaga catagattcc
    14341 acctcctata attaccatag ttgatgaccc gacgtcacga gcaagtacaa tttccacatt
    14401 ttaaaggaat ttattatact aggcgtctta ttgttgtgat ttcgtaccat gttgatttac
    14461 aaaatcactc aaattaagta acactcctac taaacgtcta ctctgttatt tcagaatgaa
    14521 tttgttgtaa tttatcttca acttcagtaa tctctgtcgc acattctttc aataaatctc
    14581 gatacttttc cgtctctgca ttgtttttat aacgtatttt atgttctaaa cttgcccaca
    14641 tatccatacc tatcgttcta atttgaattt caacaggcaa tacctcaacg cgttctgcta
    14701 aaaagactgg aatagataca acgatatgta aactgcggta accattttct ttagggtgct
    14761 gaatataatc tttacgtttt atcaattgta cgtcttcttg tttaagcaac atctcttcaa
    14821 tcaaataaat atcatctaag taattacata ctacgcgaat tccggcaata tccagtatat
    14881 gttctttggc agtttctgcg ctaatctgta atcctttacg attaagcttt tctattaaac
    14941 tacgcatttc tttcacacgt cgctccatat gatgtatagg attgtgttta taaatgtgat
    15001 tgaaattgtc atctaaaata ctcagtttag tgcttatttc ctttagtgct gacgaataaa
    15061 tatgatctaa ctctacgaaa cctaataacg tatcaaatgc ttcatcacca ttttcaaatt
    15121 tacttaaact atttttaaaa tcatgtcgca aatcctctaa atatagtgat ggttttcgat
    15181 ctacatacat ttttatacta acctccgata tataatcatc tttattgtac ctaacatttt
    15241 attaagataa tactaataca ctatcgaact ttgggctgat actgaaacag cacaaagaaa
    15301 ctcaaacaat tcgaattatg tatcaaaacc ttcaattata ggaagtgatt ttaaaatgat
    15361 ttaacgcaaa aaaacacctg ttaccgttat ataggtacaa gtgcttaatt gatagagatg
    15421 ttatacgtct ttaaatgatt ccacaacttt tggatgtgga ccatccataa gcggttctct
    15481 ttgtcgaacg ccaccacttt ggttgccaat tgattcgcta tcaaagtacg atttatcttg
    15541 atttgattgt tcttgaatat gttcttcatt atcagttgtt gcatattcac tatccacctc
    15601 tgttttttcc attgttgctg tatggaattg tacgaagttc tcttcttcta atgctttgat
    15661 ttcttctttc gataacgctc gataccaatc tttggctttt ttcgccgcaa taggaacaac
    15721 atctttttca agatatcctt cttttaacat tgcattcgca atttctatcg ctttttgccg
    15781 tttaattact tcaaattctt tccatttttc ggggtaacct ttcattgtaa agggcatttc
    15841 cttaacctcc aatatattat aattcatgtt atataccacc ataattataa aatgaaacat
    15901 attaaacaca attttttaga ctcccgtcat aataacttca gacaaacgtt agtctgacct
    15961 gataaatatt taattttaaa cgttcttgtc tctctcacga aaaccattgt gttgaatatt
    16021 catcattgct gattgaatat aattatactg tggtaaatct ggtaatatct ttatttctaa
    16081 ttctgtattt aattcgaaat gcttagcgat gttttcaaat attgctaaat actcgcccat
    16141 taattgttca tttattgtaa gtctatcttc attagccatg gctctattta acataaagct
    16201 aatttcttct aatgcgaata agctaggata ataattttga atcaatgtct tatcactaaa
    16261 taattcacca ttagctgcat tataaacttg cgtcatgtta tttaatttca cagataggtt
    16321 catagccgtg tttttctgat acacatcatc tttatattgg ttttcagaaa ataaataatg
    16381 aaagagcgtt gcttctttac gaacaacttc agccattaca ttaggaagca atgcggatgc
    16441 ggtacgttgt ccaaggacaa ataagcctat gattgcaatc acaataccca ttgcaacgtc
    16501 aataactctt ggaaacgcaa tgttaattgt taaattctgt gatgctaatc cattcattaa
    16561 aataacttgt attgtaataa aaactactgc aatcgcataa tttgctccca ccaatgcttc
    16621 agtaaacaat gctgcaatgc ccattaaaat tactgcaaca ggtgttggta ttgaaaacaa
    16681 caatatgact gaaagtacta acacacctaa aatagtacct aaacctcgtg ccatacctct
    16741 ctcgattgca tgtatagttg atgttcctag taatattgta tgtgcagata acggtatcca
    16801 gtatgctttt tcaaaatcaa acattaacgc aataaatatc gctatcatca taatcgctgt
    16861 atatctcaat gtatttctaa aaacaataga gtctaatgtt aaattttgat aaatgcgttt
    16921 actatataaa ggttttcgca tgtctgcttc atacgcaata cgcgcttcat ttgcatgcac
    16981 catttcatca attttcaata tatggttgaa taaattttga aattcctctg ttactgtcac
    17041 ttcttttcgc caatatctta cattttcctc actattatct agttgtgcaa tgatatgatc
    17101 catcatttca attaactcat ctggcaatgg tcgaatttgt ttagcgttca actctaacag
    17161 ttcagaataa atcccttggg cagatgtgtg taataataat aatttttgga aacgtctact
    17221 taatttatcg ttacttgaag tagaagtgat taataattta tcagatgctt taaaactatc
    17281 caccgctgtt tttgctactt gtaaaaatgc agatttatca ttataagtgt gtaacaactt
    17341 agatatgagt ttaaaatcat tttgaattgc ttgttcttca gctttgtttt tagaaaatac
    17401 gatcgtgatt aacaccatca tagtggcaag tataccgcct actaaaatcg caaagcctct
    17461 atataatgct tcttcgggag ctataggtaa gttaatgggt agactgaatg tcacaatgaa
    17521 gaatgtcgat gacggtccag ctatttttaa ggcagtaaat atataaaatg gtataaccgt
    17581 aacaattaat aatgtcattc caaaaacgag tggcgttttg gctgttagcg taccaagcat
    17641 catacatatt gcaaacgcta aattacaaat tattacagtt cgcagcttag atcgcgacgg
    17701 acctttaaaa acataaatat gggctagtgt tccggttgca actaataatc caaattggaa
    17761 attaccacat aagtaaccga ttattgcagg aataatcatt aacagtccct gtcttacacc
    17821 tttataaaag tctaagttaa gtcgattgat ttttgtgagt gatgtcaaaa agatgtcatt
    17881 tcgacttctc ctttccttgt caaaatattt ccataatctt tttattagca tagttaaaat
    17941 gaatttgttg ctataaattt taattacgca acatatgtca ttataacacc ttatgctcaa
    18001 ttatctaaat ttaaaaactt ttaaagtcca attaaaatta atttctaatg ttatttaaca
    18061 tcgttgaacc tttggtcgcc cacttttgcg ctagttaaaa tttatatgta cttttacaag
    18121 cgttacctaa ctaatgtttt aaaactttat ttctttacat atatcatttt aataacttcc
    18181 ttttcatctt ataccatatc cgttttaaaa agtttcaatt tcaacgtaaa aaaagcaata
    18241 agcctcaaca atgtgaaagc ttactgcttg tttaaattat ttttctattg ctttatcatc
    18301 actaacaatt aaaggttgat ctggtttcac atagaaccac ataatcacag cgattactgc
    18361 tggaacgatt aacaattgga atgtcattgt ccaacccaaa tgttctacga atagacctgc
    18421 taatagcgga ctgattaatg cacctatatt accccataag ttcatccacc ctgatactgt
    18481 accagagaag ttacgtccta agtcagttgc tgcagcccaa ctcataccca ttgaaatacc
    18541 gataccacca agacctagtg ataaccagaa aatacttaca tataaatttc ctgtgcgtac
    18601 agcaaagata attgaaactg caaacacaat aaatcctgca attgcaatta cacctctagc
    18661 tacgaatttt gaacgaccta atcctaatac acggtcagaa attgcacctg ctgataagat
    18721 taagaagaac attaataacc aaggtaatga actaatgctc atttctttaa agttaacgtg
    18781 gaatacttct gttaaatacg tcggtaacca aattaagaat aatgtaatga taaattgtac
    18841 aacaaagtat tgaattgcaa ttgcatagaa gctaaaacgt ttaaagaaat cattccatgg
    18901 tggtgatgac ttttcagtag ctacgatatc acgattttcc ataatgaaac gtttctccgc
    18961 ttcattaacc attctatgtt gctcaggtaa gtctttggca ataatcgccc ataatacagc
    19021 cattaaaata cctactgcac caaaaatgta aaatactgct tgccagttaa atgcgttaac
    19081 aatagcaatt gtaactattg gtgctaatac aggtccgaaa tatgatcctg ctaataatgc
    19141 acttgatgct ctaccttttt catttttaga gaaccaaaat gaattaaaca cagcattaga
    19201 agggtacatt ggcgcctcac ctacaccaaa taagaatctc actaaataaa ttaaaccgtg
    19261 gttcttaatc atacccgtta agattgtaaa tgcactccac caaactaatg caattgtaat
    19321 catcttacgt ggaccgaatt tttcagccaa catccccgat ggtacttgca ttaatgcata
    19381 acctagagag aagaatgatg ccaataagcc aaattgtggt ttggtcatcc ctaaatcatc
    19441 catcatttgt ttagcgatat atgaaatgtt tgctctatcc atatacgcaa taaccccaat
    19501 aaagaagaac gcaagcgcga acatccacct tacgttagtt cgtttttcct tcataactat
    19561 tcctacttcc cttattaatt tttaaaaact taccctagaa cgataaaact ttcggacatc
    19621 tggcatgtcc acccctactt tgtcacaaaa ttgtaccaaa gttatttttt ataatattat
    19681 cagacatgga aagcgtttac aatagaatct ataaaaattt tattaaattc cgcttaactt
    19741 ttgctattaa aacgatataa aggaactttt agttacatag tagcctatcg tttgttaatt
    19801 aaaaatactg caatcctagt gttttaaaac gtatattgca ggctttctta catattcgac
    19861 attcatttta aattgcataa aactacattg ctcattcatt gtgcagcgcc tgttatttag
    19921 atgatcattt gttgactttt cgtacttata gcaagcaatt ttttgattta ttgcttataa
    19981 tacattttaa gacaaatgct tgtttcaaca tcgcatacga catcattcat tattcatttg
    20041 aatttatcca gtttctattt gtc
//
LOCUS       DAHHYJ010000034        19804 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000034 DAHHYJ010000000
VERSION     DAHHYJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19804
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.034"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..688
                     /gene="bshC"
                     /locus_tag="NBK22_000937"
     CDS             <1..688
                     /gene="bshC"
                     /locus_tag="NBK22_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCD2414138.1"
                     /translation="YIKEEIINIAENQPELFSNNVVTRPLMEEWLFNTVAFVGGPSEI
                     KYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSKYNIPLEKVLVDGVEGERSK
                     FIREQASHQFIEKVEGMIEQQRRLNQDLLDEVAGNQNNINLVNKNNEIHIQQYDYLLK
                     RYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQILNDFGTDVFKPSTYPPLS
                     YTFDHIIIKP"
     gene            832..1263
                     /gene="mraZ"
                     /locus_tag="NBK22_000938"
     CDS             832..1263
                     /gene="mraZ"
                     /locus_tag="NBK22_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCD2414139.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            1279..2214
                     /gene="rsmH"
                     /locus_tag="NBK22_000939"
     CDS             1279..2214
                     /gene="rsmH"
                     /locus_tag="NBK22_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCD2414140.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            2228..2629
                     /gene="ftsL"
                     /locus_tag="NBK22_000940"
     CDS             2228..2629
                     /gene="ftsL"
                     /locus_tag="NBK22_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCD2414141.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            2610..4844
                     /locus_tag="NBK22_000941"
     CDS             2610..4844
                     /locus_tag="NBK22_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD2414142.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVVAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKYKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            5136..6101
                     /locus_tag="NBK22_000942"
     CDS             5136..6101
                     /locus_tag="NBK22_000942"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCD2414143.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            6103..7452
                     /locus_tag="NBK22_000943"
     CDS             6103..7452
                     /locus_tag="NBK22_000943"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCD2414144.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            7468..8787
                     /locus_tag="NBK22_000944"
     CDS             7468..8787
                     /locus_tag="NBK22_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCD2414145.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQAEFDEEIYLINKDFKKEESNDENNDSASSHANNNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNGIAFDNEQPQSAPKEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVSYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            8893..10305
                     /gene="ftsA"
                     /locus_tag="NBK22_000945"
     CDS             8893..10305
                     /gene="ftsA"
                     /locus_tag="NBK22_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCD2414146.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKDKEESKFKKLMKSLFE"
     gene            10338..11510
                     /gene="ftsZ"
                     /locus_tag="NBK22_000946"
     CDS             10338..11510
                     /gene="ftsZ"
                     /locus_tag="NBK22_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCD2414147.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            11770..12561
                     /gene="pgeF"
                     /locus_tag="NBK22_000947"
     CDS             11770..12561
                     /gene="pgeF"
                     /locus_tag="NBK22_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCD2414148.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDVMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAAL
                     LIYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            12585..13253
                     /locus_tag="NBK22_000948"
     CDS             12585..13253
                     /locus_tag="NBK22_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCD2414149.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            13250..13813
                     /locus_tag="NBK22_000949"
     CDS             13250..13813
                     /locus_tag="NBK22_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCD2414150.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            13825..14115
                     /locus_tag="NBK22_000950"
     CDS             13825..14115
                     /locus_tag="NBK22_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCD2414151.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYF
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            14225..15004
                     /locus_tag="NBK22_000951"
     CDS             14225..15004
                     /locus_tag="NBK22_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCD2414152.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEQHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIDYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            15028..15645
                     /locus_tag="NBK22_000952"
     CDS             15028..15645
                     /locus_tag="NBK22_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCD2414153.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            15866..18619
                     /gene="ileS"
                     /locus_tag="NBK22_000953"
     CDS             15866..18619
                     /gene="ileS"
                     /locus_tag="NBK22_000953"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCD2414154.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            18909..19706
                     /locus_tag="NBK22_000954"
     CDS             18909..19706
                     /locus_tag="NBK22_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2414155.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDILGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNNLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDC
                     VSATKRVENFDTYGLAIVDFHYPETTHLNLQGPDGIYYRFNHIEVED"
BASE COUNT         7315 a         2715 c         3828 g         5946 t
ORIGIN      
        1 atatataaag gaagaaatta taaatattgc ggaaaatcaa cctgaattat tttctaataa
       61 tgtagtgaca agaccattaa tggaagaatg gttatttaac acggtggcat ttgttggagg
      121 accgagtgaa attaagtact gggctgaact aaaagatgta tttgaactat ttgatgttga
      181 aatgcctatc gtgatgccaa ggcttagaat tacttattta aatgaccgta tagaaaaatt
      241 actttcgaaa tacaatattc cattagaaaa agtgttagtc gatggtgttg aaggagaaag
      301 aagtaagttt attagagaac aagcatcaca tcaatttatt gaaaaggtag aaggtatgat
      361 tgaacaacag cgtcgtctaa accaagactt attagatgaa gtggcgggga atcaaaataa
      421 tattaacctt gtgaataaaa ataatgaaat tcatatacaa cagtatgatt atttgttaaa
      481 acgttatctt ttaaacattg aaagagaaaa cgacatcagt atgaagcaat ttagagaaat
      541 tcaagaaaca ctccatccaa tgggaggatt acaagaaaga atatggaatc cacttcaaat
      601 tttgaatgat tttgggacag atgtgttcaa gccctccacc tatccaccac tttcttacac
      661 ttttgatcat attattataa aaccttaata taccaagggt ttagcccgat ttatcttaat
      721 gataaatcgg gcattttttt gttttttaaa ataaatttca caaattttgt ataaatagtg
      781 gtggatagtg gggagatgtg gtaaattata tataaggtga ggtgataaaa aatgttcatg
      841 ggagaatacg atcatcaatt agatacaaaa ggacgtatga ttataccgtc caagtttcgt
      901 tatgacttaa atgagcgttt tattatcaca agaggccttg ataaatgttt attcggttac
      961 actctagacg aatggcaaca gattgaagag aaaatgaaaa ccttacctat gacaaaaaaa
     1021 gacgcacgta agtttatgcg tatgttcttc tctggtgctg ttgaagtaga acttgataag
     1081 caagggcgta ttaacatccc tcaaaacttg aggaaatacg ctaatttaac taaagaatgt
     1141 acagtaatcg gtgtttcaaa tcgtattgag atttgggata gagaaacttg gaatgatttc
     1201 tatgaagaat ctgaagaaag tttcgaagat attgctgaag atttaataga ttttgatttt
     1261 taaaatggag gaattgaagt gtttcatcat atcagcgtta tgttaaacga aaccattgat
     1321 tatttaaatg taaaagaaaa tggtgtgtac attgactgta cgctaggtgg agcgggacat
     1381 gccctttatt tactaaatca attaaatgac gacggaagat taatagcaat cgatcaagac
     1441 caaactgcaa ttgataatgc taaagaggta ttaaaggatc atttgcataa ggtgactttt
     1501 gttcatagca acttccgtga attaactcaa atattaaaag acttaaacat tgaaaaagta
     1561 gatggaattt attacgactt gggtgtttca agtccccaac tcgacattcc agaacgagga
     1621 ttcagttatc accatgacgc aacattagac atgcgtatgg accaaacaca agaactaaca
     1681 gcatatgaaa ttgttaacaa ttggtcatat gaagcgttag tgaagatttt ttatcgttat
     1741 ggcgaggaga aattttcaaa acagatagct cgaagaatcg aagcacatcg cgaacaacaa
     1801 ccaataacaa caacattaga attagttgac attataaaag aaggtattcc tgcaaaagca
     1861 agaagaaaag gcggacatcc tgcaaaacga gtatttcaag cactacgaat tgcagtaaac
     1921 gatgaattgt cagcttttga agattcaata gaacaagcga ttgaattagt gaaagtagac
     1981 ggcaggattt cggtaatcac tttccattct ttagaagatc gtttatgtaa acaggtgttc
     2041 caagaatatg aaaaaggtcc agaggtacca agaggattac cagttatacc agaagcatat
     2101 acacctaagt taaagcgtgt taatcgtaaa ccgattaccg ctacagaaga agatttagat
     2161 gacaataaca gagcacgaag cgcgaaatta cgtgtagctg aaatacttaa ataaggagca
     2221 atttataatg gctgtagaaa aagtgtacca accatatgac gaacaagttt ataatagtat
     2281 accgaagcaa caaccacaaa ctaagcccga aaagaagact gtttcgagaa aagtggttgt
     2341 acaattaact aaatttgaaa aagttttata cataactttg attactgtaa ttgctatgtt
     2401 aagtatttat atgctatctt taaaaatgga tgcgtatgat acgcgaggaa agattgcaga
     2461 tttagattat aaaatagata aacaatcaag tgaaaacagt gctttacaat ctgaaatcaa
     2521 aaagaattct tcttatgaac gcatatacga aaaggctaag aaacagggga tgagccttga
     2581 gaacgataat gtaaaggtag tgcgtagtaa tggcgaagca aaaaattaaa attaaaaaaa
     2641 ataaaatagg ggcagtccta cttgttggtt tattcggact gctctttttt atattggttt
     2701 taagaatttc atatatcatg attactggac attctaatgg tcaagattta gtcatgaagg
     2761 caaatgaaaa gtatttagtt aagaatgcac aacaaccaga acgaggaaag atatatgatc
     2821 gtaatggtaa agtgctagca gaagatgtag aaagatataa acttgttgca gtaatagata
     2881 aaaaggcgag tgccaattct aaaaaaccta ggcatgtagt tgataaaaaa gagactgcaa
     2941 agaaattatc tacagtcatt gatatgaagc cagaggaaat tgaaaagaga cttagtcaaa
     3001 agaaagcttt ccaaattgaa tttggacgca aaggaacaaa tttaacgtat caggacaaat
     3061 tgaaaataga gaaaatgaat ttgcctggta tttctttatt gcctgaaaca gaacgctttt
     3121 atccaaatgg caattttgca tcacacttaa ttggtagagc tcagaaaaat ccggatactg
     3181 gtgaacttaa aggtgcactt ggagttgaaa agatttttga tagttattta agtggatcta
     3241 aaggatcatt gagatatatt catgatattt ggggatatat cgcaccaaat actaaaaaag
     3301 agaagcagcc taaacgtggt gatgatgtcc atttaacaat cgattcaaat attcaagtat
     3361 ttgttgaaga agctttagat ggcatggttg aaagatacca gccgaaagat ttatttgcgg
     3421 ttgtcatgga tgccaaaact ggagaaattt tagcatacag tcagcgacca acatttaatc
     3481 ctgaaactgg taaagacttt ggtaaaaagt gggcaaatga cctatatcaa aacacatacg
     3541 agcctggatc aacatttaaa tcatatgggt tagcagctgc tattcaagaa ggtgcttttg
     3601 atcctgataa gaaatataaa tctggacata gagatattat gggttcacgt atttcagact
     3661 ggaatagagt cggttggggt gaaatcccaa tgtcactcgg atttacttat tcatctaata
     3721 cattgatgat gcatttacaa gatttagttg gtgcagacaa aatgaaatct tggtatgaac
     3781 gatttggatt tggaaaatca actaaaggta tgtttgatgg agaagcacct ggtcaaattg
     3841 gatggagtaa tgagttgcaa caaaaaacgt catcatttgg tcaatcgaca acagtaacac
     3901 ctgttcaaat gttacaagcg caatcagcgt tctttaatga tggtaatatg ttaaaaccat
     3961 ggtttgtgaa tagcgttgaa aatcctgtta gtaaaagaca attttataaa ggtcaaaaac
     4021 aaatcgcagg caaaccaata acaaaagata ctgctgaaaa agttgaaaag caattagatt
     4081 tagttgtgaa tagtaagaag agtcacgctg caaactatcg tattgatggt tatgaggtcg
     4141 aaggtaagac tggtacagca caagtcgctg cacctaatgg tggtggatac gttaaaggtc
     4201 caaacccata ttttgtaagt tttatgggtg acgcgccgaa gaaaaatcct aaagttattg
     4261 tatacgctgg tatgagcttg gcacaaaaaa atgaccaaga agcttatgaa ttaggtgtta
     4321 gtaaagcgtt taaaccaata atggaaaata ctttgaaata tttaaatgta ggtaaatcaa
     4381 aagatgacac atctaatgca gagtatagta aagtgccaga tgttgaaggt caagacaaac
     4441 aaaaagctat tgataatgtg agtgcaaaat cattagaacc agttactatt ggttctggca
     4501 cacaaataaa agcacaatct ataaaagcag ggaataaagt cttacctcat agtaaagtac
     4561 tgttattaac agatggagac ttaactatgc ctgacatgtc aggatggacg aaagaagatg
     4621 ttgttgcttt tgaaaaccta acaaatatta aagtaaattt aaaaggtagc ggttttgtgt
     4681 cccaccaatc aattagtaag ggacaaaaac ttactgaaaa atataaaata gacgtagaat
     4741 tttcatcaga gaatgtagac agcaattcga cgaataattc tgattcaaat tcagatgata
     4801 agaagaaatc tgacagtaaa actgacaagg ataagtcgga ctaacaagaa tggctacatt
     4861 aaataaggat gtattgtcac tatttgtttt tactaaagta atgctgtgat agaaaacaac
     4921 atcttcattt aatctttata aataagtgtt gatagagaat tttaaggtag acgaggtcag
     4981 attaacttgt ttgttatagg caaacttttt atactggcct cttattccgt aaaaaagaaa
     5041 aattataaat tttgattaca actttgttta tttaattgaa aaattgataa tatattagtg
     5101 gttaaaaaag tctggattaa gaagaggaga tagttatgat ttttgtatat gcgttattag
     5161 cgctagtgat tacatttgtt ttggtacctg ttttgatacc tacattaaaa aggatgaaat
     5221 ttggtcaaag tattcgagaa gaaggtccac aaagccatat gaagaagact ggtacaccaa
     5281 cgatgggtgg actaacattt ctattaagta ttgtgataac gtctttggtg gctattatat
     5341 ttgtagatca agctaatcca atcatactgt tattatttgt gacgattggt tttgggttaa
     5401 ttggttttat agatgattat attattgttg ttaaaaagaa taaccaaggt ttaacaagta
     5461 aacagaagtt tttggcgcaa attggtattg cgattatttt ctttgtttta agtaatgtat
     5521 ttcatttggt gaatttttct acgagcatac atattccatt tacgaatgta gcaatcccac
     5581 tatcatttgc atatgttatt ttcattgttt tttggcaagt aggtttttct aatgcggtaa
     5641 atttaacaga tggtttagat ggattagcaa ctggactgtc aattatcgga tttacaatgt
     5701 atgccatcat gagctttgtg ttaggagaaa cggcaattgg tattttctgt ataattatgt
     5761 tgtttgcact tttaggattt ttaccatata acattaaccc tgctaaagtg tttatgggag
     5821 atacaggtag cttagcttta ggtggtatat ttgctacgat ttcaatcatg cttaatcagg
     5881 aattatcatt aatttttata ggtttagtat tcgtaattga aacattatct gttatgttac
     5941 aagtcgctag ctttaaattg actggaaagc gtatatttaa aatgagtccg attcatcatc
     6001 attttgaatt gataggatgg agcgaatgga aagtagttac agtattttgg gctgttggtc
     6061 tgatttcagg tttaatcggt ttatggattg gagtgcatta agatgcttaa ttatacaggg
     6121 ttagaaaata aaaatgtatt agttgtcggt ttggcaaaaa gtggttatga agcagctaaa
     6181 ttattaagta aattaggtgc gaatgtaact gtcaatgatg gaaaagactt atcacaagat
     6241 gctcatgcaa aagatttaga atctatgggc atttctgttg taagtggaag tcatccatta
     6301 acattgcttg ataataatcc aataattgtt aagaatcctg gaatacctta tacagtatct
     6361 attattgatg aggcagtgaa acgaggtttg aaaattttaa cagaagttga gttaagttat
     6421 ctaatctctg aagcaccaat catagctgta acgggtacaa atggtaaaac gacagttact
     6481 tctctaattg gagatatgtt taaaaaaagt cgcttaactg gaagattatc cggcaatatt
     6541 ggttatgttg catctaaagt agcacaagaa gtaaagccta cagattattt agttacagag
     6601 ttgtcgtcat tccagttact tggaattgaa aagtataaac cacacattgc tataattact
     6661 aacatttatt cggcgcatct agattaccat gaaaatttag aaaactatca aaatgctaaa
     6721 aagcaaatat ataaaaatca aacggaagaa gattatttga tttgtaatta tcatcaaaga
     6781 caagtgatag agtcggaaga attaaaagct aagacattgt atttctcaac tcaacaagaa
     6841 gttgatggta tttatattaa agatggtttt atcgtttata aaggtgttcg tattattaac
     6901 actgaagatc tagtattgcc tggtgaacat aatttagaaa atatattagc agctgtgctt
     6961 gcttgtattt tagctggtgt acctattaaa gcaattattg atagtttaac tacattttca
     7021 ggaatagagc atagattgca atatgttggt actaatagaa ctaataaata ttataatgat
     7081 tccaaagcaa caaacacgct agcaacacag tttgccttaa attcatttaa tcaaccaatc
     7141 atttggttat gtggtggttt ggatcgaggg aatgaatttg acgaactcat tccttatatg
     7201 gaaaatgttc gcgcgatggt tgtattcgga caaacgaaag ctaagtttgc taaactaggt
     7261 aatagtcaag ggaaatcggt cattgaagcg aacaatgtcg aagacgctgt tgataaagta
     7321 caagatatta tagaaccaaa tgatgttgta ttattgtcac ctgcttgtgc gagttgggat
     7381 caatatagta cttttgaaga gcgtggagag aaatttattg aaagattccg tgcccattta
     7441 ccatcttatt aaagggtgtg agtattgatg gatgataaaa cgaagaacga tcaacaagaa
     7501 tcaaatgaag ataaagatga attagaatta tttacgagga atacatctaa gaaaagacgg
     7561 caaagaaaaa gatcaaaggc tacacatttt tctaatcaaa ataaagatga tacatctcaa
     7621 caagctgagt ttgatgaaga aatttacttg ataaataaag acttcaaaaa agaagaaagc
     7681 aatgatgaaa ataatgattc tgcttctagt catgcgaata ataataatat cgatgattct
     7741 acagactcta atattgaaaa tgaggattat agatataatc aagaaattga cgaccaaaat
     7801 gaatcgaatg gaattgcatt cgacaacgaa caacctcaat cagctcctaa agaacaaaat
     7861 agcgactcga atgatgagga aacagtaacg aaaaaagaac gaaaaagtaa agtaacacaa
     7921 ttaaagccat taacacttga agaaaagcgg aagttaagac gtaagcgaca aaaacgaatc
     7981 caatacagtg ttattacaat attggtattg ttgattgctg ttatattaat ttacatgttt
     8041 tcaccactta gtaaaattgc gcatgtaaat ataaatggaa ataatcacgt tagtacttca
     8101 aagataaaca aagttttagg tgttaaaaat gattcaagga tgtatacgtt tagtaaaaaa
     8161 aatgctatta atgatctcga agaggatcca ttaatcaaaa gtgttgagat acacaagcaa
     8221 ttaccaaaca cattaaacgt agatatcaca gaaaatgaaa ttattgcttt agtgaaatat
     8281 aaaggtaaat atttaccttt attagaaaat ggtaaattgc ttaaaggttc aaatgatgtc
     8341 aaaattaatg atgcacctgt catggatggt ttcaaaggta caaaagaaga tgatatgatt
     8401 aaggcgttat ctgaaatgac acctgaagtt agacgatata ttgccgaagt gtcatacgcc
     8461 ccaagtaaaa acaaacaaag cagaattgaa ttgtttacga cggatggact tcaagtaatc
     8521 ggtgatattt cgacgatatc taagaaaatg aaatattatc cgcagatgtc acaatcatta
     8581 tcaagggata gttcgggtaa actaaaaaca cgaggctata ttgatttatc agtcggtgct
     8641 tcatttatcc cataccgtgg aaacacgtct agtcaatcag aaagcgataa aaatgtgact
     8701 aaatcatctc aagaggaaaa tcaagcaaaa gaagaattac aaagcgtttt aaacaaaatt
     8761 aacaaacaat caagtaagaa taattaaaaa attttgatat tgtctatgtt tatagttcac
     8821 aagccattca acgtattgta aactaaggat agtgtatttt tttaatagta atttgtcagg
     8881 aggtgcctat ctatggaaga acattactac gtaagtattg atattggatc atcaagcgta
     8941 aaaacaatag taggcgagaa atttcacaat ggtataaatg tgataggtac aggacaaacc
     9001 tacacgagcg gtataaaaaa tggtttaatt gatgattttg atattgcgcg acaagcaatc
     9061 aaagacacaa ttaaaaaggc atcaatcgct tcgggtgttg atattaaaga agttttcctg
     9121 aaattaccta tcattggaac ggaagtttat gatgaatcaa atgaaatcga cttttatgag
     9181 gatacagaaa tcaacggttc acatatcgaa aaagtattag aaggtattag agaaaaaaat
     9241 gatgtgcaag aaacagaagt aattaatgtg ttcccgattc gttttatagt cgataaagaa
     9301 aatgaggttt cagaccctaa agaattaatt gccagacatt cattaaaggt tgaagcaggc
     9361 gtaattgcta ttcaaaaatc gattttaatt aatatgatta aatgcgtaga agcatgtggt
     9421 gttgatgtat tagatgttta ctctgatgca tataactatg gttcaatcct aacagctact
     9481 gaaaaagagt taggtgcatg tgtcattgat attggtgaag acgttacgca agttgctttt
     9541 tatgaacgcg gtgaattagt agatgctgat tctatcgaaa tggcagggcg tgatattaca
     9601 gacgatattg cacaaggatt aaacacttct tatgaaactg ctgaaaaagt taaacaccaa
     9661 tatggtcatg cattctatga ttctgcttca gatcaagata tcttcactgt tgaacaggtt
     9721 gatagtgatg aaacagtaca gtatactcaa aaagatttga gtgactttat tgaagcgcgt
     9781 gtagaagaaa tattcttcga agtatttgat gttttacaag atttaggatt aacaaaagta
     9841 aatggtgggt ttattgtaac tggtggatct gcaaacttac ttggcgtaaa agaattatta
     9901 tcagatatgg taagtgaaaa agttagaatt cacacgccat cacaaatggg aattagaaaa
     9961 cctgaatttt cttcagcaat ttctacaatt tctagtagta tcgcttttga tgagttatta
    10021 gattatgtta caattaatta tcatgataat gaagaaactg aagaagatgt tattgatgtg
    10081 aaagacaaag ataacgaatc taaattaggc ggatttgatt ggtttaaacg taaaacaaac
    10141 aaaaaagata ctcatgaaaa tgaagtagag tcaacagatg aagaaattta tcaatcagaa
    10201 gataatcatc aggaacataa acagaatcat gaacatgttc aagacaaaga taaagataaa
    10261 gaagaaagta aattcaaaaa actaatgaaa tctctatttg aatgattatt ggccaataaa
    10321 actaggagga aatttaaatg ttagaatttg aacaaggatt taatcattta gcgactttaa
    10381 aggtcattgg tgtaggtggt ggcggtaaca acgccgtaaa ccgaatgatt gaccacggaa
    10441 tgaataatgt tgaatttatc gctatcaaca cagacggtca agctttaaac ttatctaaag
    10501 ctgaatctaa aatccaaatc ggtgaaaaat taacacgtgg tttaggagca ggagctaatc
    10561 ctgaaatcgg taaaaaagct gcagaggaat ctcgtgaaca aattgaagat gcaatccaag
    10621 gtgcagacat ggtatttgtt acttctggta tgggtggcgg aactggtact ggtgcagcac
    10681 cagtcgttgc taaaattgca aaagaaatgg gcgcattaac tgttggtgtt gtaactcgtc
    10741 catttagttt tgaaggacgt aaacgtcaaa ctcaagctgc tgctggagta gaagctatga
    10801 aagctgcagt agatacatta atcgttatac caaatgaccg tttattagat atcgttgaca
    10861 aatctacgcc aatgatggaa gcatttaaag aagctgacaa cgtgttacgc caaggtgtac
    10921 aaggtatctc agacttaatc gctgtttctg gtgaagtaaa cttagacttt gcagacgtta
    10981 agacaattat gtctaaccaa ggttctgcat taatgggtat tggtgtttct tctggtgaaa
    11041 atagagcggt agaagctgct aaaaaagcaa tctcttctcc attacttgaa acatctatcg
    11101 ttggtgcaca aggtgtgctt atgaatatta ctggtggcga gtcattgtca ttatttgaag
    11161 cacaagaggc tgctgatatt gtccaagatg ctgcagatga agacgttaat atgattttcg
    11221 gtacagttat taatcctgaa ttacaagatg agattgttgt aacagttatt gcaactggtt
    11281 ttgatgacaa accaacatca catggtcgta aatctggtag cactggattc ggaacaagcg
    11341 taaatacttc tagcaatgca acttctaaag atgaatcatt cacttcaaat tcatcaaatg
    11401 cacaagcaac tgatagtgta agtgaaagaa cacatacaac taaagaagat gatattccta
    11461 gcttcattag aaatagagaa gaaagacgtt caagaagaac aagacgttaa tcggttaata
    11521 tatatacaca aataattcaa cacaaatcat cagataacat atctgatgat ttttttacta
    11581 atttttagaa catgtagaag gacatttaag tttttcaaag ttattaaaag tgtttaagta
    11641 tcgtgtgaaa attaagtcaa aaattatttg cgcaacattt taactttaaa cataaatgtt
    11701 atattatata attattaact ttgtacagtt agacgaagat aatttaaatg aaatgatggt
    11761 gacgatcgag tgaatgataa ttttaaaaag caaccgcatc atttaatata tgaagagtta
    11821 ttacaacaag gtattactct aggtattaca actagaggag atggtttaag tgactatcct
    11881 aaaaatgctt ttaatatggc gagatatatt gatgatcgcc catataatat tactcaacat
    11941 caattgcaat tagctgaaga aattgcgttt gatagaaaaa attgggtgtt tcccattcaa
    12001 acacatgaaa ataaagtcgc ttgtattaca aaggatgata taggcacaaa tatagacact
    12061 ttaactgatg cgcttcatgg tattgatgtg atgtacacat atgatagtaa tgtcttatta
    12121 acgatgtgtt atgcagactg tgtaccagta tatttttata gtacaaaaca tcattttatt
    12181 gcattggcgc atgcaggttg gcgtggtacc tatactgaaa ttgtaaaaga agtgctaaaa
    12241 catgtgaact ttgatttgaa agacttacat gtcgttattg gaccatctac atcatcaagt
    12301 tatgaaatta atgatgatat taaaaataaa tttgaaacat tgccaattga tagtgccaac
    12361 tatattgaaa ctagaggacg agatcgtcat ggtattgatt tgaaaaaagc caatgctgca
    12421 ttattaattt attatggtgt tcctaaagaa aatatttata cgacagcgta tgctacatct
    12481 gaacatttag aattattttt ctcttatcga ttagaaaaag gtcaaacagg acgcatgtta
    12541 gcattcattg gtcaacagta aacaaggagg agatatgttt gcgtgtgaaa gataatttac
    12601 aacaaatctc aacacaaatt aatgacaaaa gtgaaaaaaa taatttttca acaaaaccaa
    12661 acgtgattgc agttacaaaa tatgttacaa tagagcgagc taaagaagcg tatgaggctg
    12721 gaataagaca ttttggtgag aatagattgg aaggcttttt tcaaaagaaa gaagcattac
    12781 catcagatgc ggtgatccat tttataggat cattacaatc tcgaaaagtt aaggacgtta
    12841 taaacgacgt agattatttc catgctttag atcgattgag cttagccaaa gaaattaaca
    12901 aacgtgcaga acataaaatt aaatgtttct tgcaagtgaa cgtttcggga gaagcttcta
    12961 aacatggtat tgctttagaa gatgttgatc agtttataga tgatcttaaa aaatatgaca
    13021 aaatcgaaat tgtaggttta atgacgatgg caccattgac agatgatgaa gcatatatta
    13081 gatcgttatt taaacagtta cgtttgaaaa aagaagaaat acaacgactc aatttagaat
    13141 atgcgccttg tgatgaatta tcaatgggaa tgagtaatga ctatcttatt gcagttgaag
    13201 aaggtgcgac gtttgttaga attgggacta aacttgtagg agaagaggag tgagccactt
    13261 ggctttaaaa gatttattta gtggattttt tgtaatagat gatgaagagg aagtagaagt
    13321 acctgacaaa caacaacagg taaatgaagc gccagcaaaa gagcagtcac aacaaacaac
    13381 aaaacaaaac gcaatcaaat cagtccctca aaaatctgca tcaagatata caacaacgtc
    13441 agaagaaagg aataaccgta tgtctaatta ttcaaaaaat aattcacgta atgttgtaac
    13501 tatgaacaat gctacaccaa acaatgcatc acaagaaagt tcaaaaatgt gtttattcga
    13561 accacgtgtt ttttcagata cacaagatat tgctgatgag cttaaaaacc gccgtgcgac
    13621 acttgtcaat ttacaacgta ttgataaagt atcagcgaaa agaattattg attttttaag
    13681 cggtactgtt tatgcaatcg gtggagatat ccaacgtgta ggtactgata ttttcttatg
    13741 tacgcctgat aatgtggaag tagctggaag cattacagac catattgaaa atatggaaca
    13801 ttcattcgac taaggagtta acatatggat ataaatgtgc tagctacaat atttaaattc
    13861 atcctttttg ttgttgaaat ttattatttc ggcatgatta tatatttctt tacatcttgg
    13921 gtaccaagta ttagagaaac taaggtaggt tattttttag cgaaaatata tgaacctttc
    13981 ttacaaccat ttagaaaagt aattccacct attggaatta tcgacatatc atcaatcgct
    14041 gcaattatcg ttttagtatt attccaaaaa gggttactcc aaatctttaa ttggatttta
    14101 attcaattac aataaattaa gcgacataac gaggcaaggg tacatgatac tttagcctcg
    14161 tttttgatat gtatttttct gaatataagg gcaatagatg gtattttata atttttttaa
    14221 ggtagtgatt aacatagata tttatcaaca ctttagacag gaagaatacg aattaattga
    14281 tcagctaacg gataaatgtg atcaagcgga acagcattat gcaccagtat taacgcattt
    14341 tttagatcca agagggcaat atatattgga agtgatttgt ggcagttatg aagatttaaa
    14401 cgtatctttt tatggtggac ctaatgctga aagaaaaaga gcaatcattt cgccgaacta
    14461 ttatgaacct aaagaaagcg actttgaatt aactttaatg gaaatagatt atcctgaaaa
    14521 attcgtcact ttaaaacatc aacatatttt agggacatta atgtctttag gtatcgaacg
    14581 cgaacaagtt ggagatataa ttgtgaatga acgaattcaa tttgttttga caagtagatt
    14641 ggaatcattt attatgttag aattacaacg tattaaaggc gcatcagtta aactttatac
    14701 tattccagta acagatatga tacaatctaa tgagaattgg aaaaatgaaa gtgcaacagt
    14761 tagttcttta aggttagatg ttgttattaa agaaatgata cgtaaatcac gtacgattgc
    14821 gaaacaacta atcgaaaaaa aacgtgttaa agtgaatcac actattgttg attcagcaga
    14881 ttttcaatta caagcaaatg atttaatatc catccaaggt tttggtagag cacacattac
    14941 tgacttaggt ggtaaaacta aaaaagataa aacgcacatt acctatagaa cattattcaa
    15001 atagtaatga tttaaggagg ataacaaatg ccttttacac caaatgaaat taagaataaa
    15061 gagttttcac gtgtaaagaa tggtttagaa cctactgaag ttgctaattt tttggagcaa
    15121 ctaagcactg aaattgaacg tcttaaagaa gataaaaaac aacttgaaaa agtaatcgaa
    15181 gagagagata ctaatattaa gtcttatcaa gacgtgcatc aatctgtaag tgatgctttg
    15241 atacaagctc aaaaagctgg tgaagaaact aagcaagctg cagagaaaca agctgaagcg
    15301 attatagcta aggcagaagc gcaagctaat caaatggttg gtgacgcggt agaaaaagca
    15361 cgccgtttag cattccagac tgaagatatg aaacgtcaat caaaagtatt tagatcgcgt
    15421 ttccgtatgt tagttgaagc gcaattagac ttattaaaaa acgaagattg ggattacttg
    15481 ttgaattatg atttagacgc tgaacaagtg acgcttgaaa atattcatca tttgcatgaa
    15541 aatgatttaa agccagatga agttgcagca aatgcacaaa ataatgcatc aaatacacca
    15601 gacaataatc aacaatccaa tgattcagaa acaactaaga agtaagaatt aaataaagac
    15661 agacgcgtaa tatacattta acttttcaca gcgaattagg taatggtgag agcctagtaa
    15721 aagcatgtat gttatatcac tggcttttta atatttaaat aatgtaatga gagaactcta
    15781 agttgagtta ataagggtgg taccgcgagc aatcgtccct tttaatttaa cttagagttt
    15841 tttaaatttt taaggagtga aaaaaatgga ttacaaagaa acgttattaa tgcctaaaac
    15901 agatttccca atgcgaggtg gtttaccaaa caaggaaccg caaattcaag aaaaatggga
    15961 tgcagaagat caataccata aagcgttaga aaaaaataaa ggtaacgaaa cattcatttt
    16021 acatgatggc ccaccatacg cgaatggtaa cttacatatg ggacatgcct tgaacaaaat
    16081 tttaaaagac tttattgtac gttataaaac tatgcaaggg ttctatgcac catacgtacc
    16141 aggttgggat acacatggtt taccaattga acaagcatta acgaaaaaag gtgttgaccg
    16201 aaagaaaatg tcaacagctg aattccgtga gaaatgtaaa gaatttgctt tagaacaaat
    16261 tgaattacag aaaaaagatt ttagacgttt aggtgttcgt ggtgacttta atgatccata
    16321 tattacatta aaacctgaat acgaagctgc acaaattcgt atttttggag aaatggcaga
    16381 taaaggttta atttataaag gtaaaaagcc agtttattgg tctccttcaa gtgagtcttc
    16441 attagcagaa gcagaaattg aatatcacga taaacgttca gcatcaattt acgttgcatt
    16501 tgacgttaaa gatgacaaag gtgtcgttga tgcagatgct aaatttatta tctggacaac
    16561 aacgccatgg acaattccat caaatgttgc gattaccgtt catcctgaat taaaatatgg
    16621 tcaatacaat gtaaatggcg aaaaatatat tattgcagaa gccttgtctg acgctgtagc
    16681 agaagcactg gattgggata aagcatcaat caaattagaa aaagaataca caggtaaaga
    16741 attagagtat gttgtagcac aacatccatt cttagacaga gaatcgttag tgattaatgg
    16801 tgatcatgtt actacagatg ctggtacagg ttgtgtacat acagcaccag gtcacgggga
    16861 agatgactat attgttggtc aaaaatatga attgccagta attagtccaa tcgatgataa
    16921 aggtgtattt actgaagaag gcggccaatt tgaagggatg ttctatgata aagctaataa
    16981 agccgttact gatttattaa cagaaaaagg tgcactatta aaattagact ttattacaca
    17041 tagctatcca cacgactgga gaacaaaaaa acctgtaatc ttccgtgcta caccacaatg
    17101 gtttgcctca atcagtaaag taagacaaga tattttagat gcaatcgaaa atacaaactt
    17161 caaagtaaat tggggtaaaa cacgtattta caatatggtt cgtgaccgtg gcgaatgggt
    17221 tatttctcgt caacgtgtgt ggggtgtacc gttaccagta ttttatgctg aaaatggcga
    17281 aattatcatg acgaaagaaa cagtgaatca tgttgctgat ttatttgcag aacacggttc
    17341 aaatatttgg tttgaaagag aagcgaaaga cttactacca gaaggattta cacatccagg
    17401 cagccctaac ggtacattta ctaaagaaac agacattatg gacgtttggt ttgattctgg
    17461 ttcatcacac cgtggcgtgt tggaaacaag accggaatta agtttcccag cggatatgta
    17521 tttagaaggt agtgaccaat atcgtggttg gttcaactct tctatcacaa cttcagttgc
    17581 tacaagagga gtatcacctt ataaattctt actttctcat ggttttgtta tggacggtga
    17641 aggtaagaaa atgagtaaat ctttaggtaa tgtgattgta cctgaccaag tggttaaaca
    17701 aaaaggtgct gatattgcga gactttgggt aagtagtacg gactatttag ctgatgttag
    17761 aatttctgat gaaattttaa aacaaacatc tgatgtttat cgtaaaatca gaaatacatt
    17821 aagatttatg ttaggtaaca ttaacgattt caatcctgac acagatagca ttcctgaatc
    17881 agagttatta gaagtggatc gttacttgct aaatcgttta cgtgaattta ctgcaagtac
    17941 gattaacaac tatgaaaact ttgactactt aaatatttat caagaagttc aaaactttat
    18001 caatgttgag ttaagtaatt tctatttgga ttacggtaaa gatattttat atattgaaca
    18061 acgtgattct catatccgtc gtagtatgca aacagtgtta tatcaaattt tagttgatat
    18121 gacgaagttg ttagcaccaa tcttagtgca tacagctgaa gaagtttggt ctcatacacc
    18181 acatgttaaa gaagaaagtg ttcacttagc agacatgcct aaagttgtag aagtagatca
    18241 agctttattg gataaatggc gtacatttat gaatttacgt gatgatgtga accgtgcatt
    18301 agaaactgct cgtaatgaaa aagttattgg taaatcatta gaagctaaag ttacgattgc
    18361 tagtaacgat aaatttaatg catctgaatt cttaacttca tttgatgcat tacatcaatt
    18421 atttatcgtg tcacaagtta aagttgtaga taagttagac gatcaggcaa cagcttatga
    18481 acatggtgat attgtcatcg aacatgcaga tggtgaaaaa tgtgaaagat gttggaacta
    18541 ttcagaggat cttggtgctg ttgatgaatt gacgcatcta tgtccacgat gccaacaagt
    18601 tgtaaaatca cttgtataat tgaaattgta taaagtactc atacagatga tataaattaa
    18661 agctctcttc ataatcatgt tgtagttttt gttgacatga tgaagagagt ttttttgtga
    18721 ataaaaaaat gaccaagtta ccggtcatat atgtaaaaaa tgtgcgattt actaaaataa
    18781 aaattattca ggaatggtac aaattctctg aggcatataa atgcgttata gttgctattc
    18841 tcaattatgt tcgcgataat tttaagtaaa agtaagcaca gatattgaat ttgataggag
    18901 ttaattgaat gtatcataac agtaacgcaa actttgtcaa tggtatcact ttaaatgtga
    18961 gagataagaa tgaattaaag ccattttatg aggacatatt aggattaaat attataaatg
    19021 agacattaac atcgatacaa tatgaagtag gtcaaaataa tcatgtcatt acacttgttg
    19081 aattacaaaa tggacgtgaa cctttaatgt ccgaagcggg actgtttcat atcgcaatta
    19141 aactacctca aattagtgat ttagctaatt tactaattca tttaagcgaa tatgatattc
    19201 cagttaacgg aggtatacag cctgcttcgt tatcattatt ttttgaagac ccggaaggaa
    19261 acggttttaa attttatgtt gataaagacg aagcgcaatg gacgaggcaa aataatttag
    19321 taaaaattga tattagacca ttaaatgtac cgagattagt gagtcatgca acaaaattgt
    19381 tatggttagg tattccagat gacgctatta taggtgcatt gcatattaag acaattcatt
    19441 tatcagaggt aaaagagtac tacctcgatt attttggatt agagcaatcg gcatatatgg
    19501 atgattattc aatattttta gcatcgaatg gctattatca acatttggcc atgaatgatt
    19561 gcgtatcagc aacgaaacgt gtagaaaatt ttgatacgta tggattagca attgttgact
    19621 ttcattatcc tgaaacaaca catttaaatt tacaaggtcc ggatggtatc tattatcgct
    19681 ttaatcatat cgaagttgaa gattagtata tactttgaat ggacgaacca tataatgaat
    19741 cgtttttaat gatcttttta tacaagttat gaaggaggct gggacattaa gttcttaggc
    19801 aatg
//
LOCUS       DAHHYJ010000035        19591 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000035 DAHHYJ010000000
VERSION     DAHHYJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19591
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.035"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(1090..1764)
                     /locus_tag="NBK22_000955"
                     /pseudo
     CDS             complement(1090..1764)
                     /locus_tag="NBK22_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1804..1923)
                     /locus_tag="NBK22_000956"
     CDS             complement(1804..1923)
                     /locus_tag="NBK22_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCD2414156.1"
                     /translation="MQYNTTRYIDENQDNKTLKDMMKNGKQRPWREKKVLLQS"
     gene            complement(2114..2461)
                     /locus_tag="NBK22_000957"
     CDS             complement(2114..2461)
                     /locus_tag="NBK22_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2414157.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK
                     LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD
                     EIREFFSKNHEGF"
     gene            complement(2480..3097)
                     /gene="cadD"
                     /locus_tag="NBK22_000958"
     CDS             complement(2480..3097)
                     /gene="cadD"
                     /locus_tag="NBK22_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter CadD"
                     /protein_id="HCD2414158.1"
                     /translation="MIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRDIYVGQY
                     LGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKKELNE
                     KGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVFTAQK
                     LANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF"
     gene            complement(3480..3668)
                     /locus_tag="NBK22_000959"
     CDS             complement(3480..3668)
                     /locus_tag="NBK22_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414159.1"
                     /translation="MNKDTVLDKGYVIATILNVFFLLGLIFISHLENLYILIPYVILM
                     GINAIYLVVKFMNFKKNN"
     gene            complement(4239..4385)
                     /locus_tag="NBK22_000960"
     CDS             complement(4239..4385)
                     /locus_tag="NBK22_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414160.1"
                     /translation="MNTKILTGMTGSSLERKINNFINDNQIEVIDIKFSSSVFYFGVM
                     IIYK"
     gene            complement(4390..4715)
                     /locus_tag="NBK22_000961"
                     /pseudo
     CDS             complement(4390..4715)
                     /locus_tag="NBK22_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002483623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
     gene            complement(4708..5652)
                     /locus_tag="NBK22_000962"
     CDS             complement(4708..5652)
                     /locus_tag="NBK22_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCD2414161.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALL
                     KDRFELSIQNEWVDRNNNIYFIFSNKHLCEYLGYGEQKIIKLKKELVNFNLLTQERVG
                     LNKPNRLYLLKPNYDIEASLTKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTELN
                     NTNYIENEDNDTYDMNDIISNTLINNNHHSNHTNHQKTEFNNDALKFQVLEELPQQLQ
                     DYLSKFEIREIRIIKSVLLKGKKSFNTSHDTYYRLEDVEFEIVSVLKRFKAMLLQKNE
                     TVEAMQGYLMQSIKAELEETHALYMRRKNMKQYNIFNQ"
     gene            6096..6866
                     /locus_tag="NBK22_000963"
     CDS             6096..6866
                     /locus_tag="NBK22_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCD2414162.1"
                     /translation="MKTIKMVADELNVTKQTIVNNAKNLNISFKKENGINYINDNDCL
                     KIIEKITKKERTMQNKESIKKERFNENTAEFEKHIYNNSNGFESLKTKVNELEKQIEI
                     FETRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILALESNKKIQKLENQLEEER
                     QLNYSFDTATNDRQSINPQEATFTEEYQNSNQQQKKIQPIEAELNHKGITKAKKDDSA
                     NEDVLKRDDKLSEEIESGPEDREVETPKKGFWSRLFGS"
     gene            complement(7485..8255)
                     /locus_tag="NBK22_000964"
     CDS             complement(7485..8255)
                     /locus_tag="NBK22_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002475893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414163.1"
                     /translation="MGTLIKQECFKLFKKKSTFIIPIIIMLLMVVQAIISKNYDDVFS
                     PQSSIESAFSGFSWFIFLLIIQASTIISMEFYHGTIKNLLYRKYTRTSIIISKIITLI
                     LFSLLYFIVSIVVGIILWAIFFNDINLLESKGDALSLLSKMLLTGLGTYVGTWLVLSI
                     TLLISCAMKSPGVSIAVGIVLYFATSILSGILTIVVDKWEWLKWNPISMMNIMVQIVD
                     KNMKKFTKLELHELFIGNIVYIIIFLIIVVFVFKKKNV"
     gene            complement(8257..8952)
                     /locus_tag="NBK22_000965"
     CDS             complement(8257..8952)
                     /locus_tag="NBK22_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2414164.1"
                     /translation="MDVLTVEHLTKKIGNKTILKDISLNLKRGQIVGLVGANGAGKTS
                     LMKVILGYSIYQEGNFEVIENKHKKSNVGALIENPGVYPFMSGYDNLKLLNETKNTQD
                     IDTIVSQLKMDEYIHNKAKTYSLGMKQKLGIAIAFLNQPQLIILDEPMNGLDPRAVRD
                     VRELIVKKAEEGVTFLISSHILSELVKITNSILIINKGKIVTETTEEELNQYKDNDLE
                     NVLLSIIDKEDQS"
     gene            9489..10955
                     /gene="msr(A)"
                     /locus_tag="NBK22_000966"
     CDS             9489..10955
                     /gene="msr(A)"
                     /locus_tag="NBK22_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCD2414165.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIDHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETNGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLNNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRKYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALERSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSIHDIT"
     gene            complement(11179..11787)
                     /locus_tag="NBK22_000967"
                     /pseudo
     CDS             complement(11179..11787)
                     /locus_tag="NBK22_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(11914..12492)
                     /locus_tag="NBK22_000968"
     CDS             complement(11914..12492)
                     /locus_tag="NBK22_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2414166.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNQYGCEKIFSDHISGAKSKRPG
                     LDRAIEFARSGDTIVVWRLDRLGRNMADLITLVNELNNRGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNG
                     TPIKTIAEQWKVSRTTIYRYLNKLNNQENKDK"
     gene            complement(12756..13136)
                     /gene="blaI"
                     /locus_tag="NBK22_000969"
     CDS             complement(12756..13136)
                     /gene="blaI"
                     /locus_tag="NBK22_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCD2414167.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
     gene            complement(13126..14883)
                     /gene="blaR1"
                     /locus_tag="NBK22_000970"
     CDS             complement(13126..14883)
                     /gene="blaR1"
                     /locus_tag="NBK22_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD2414168.1"
                     /translation="MAKLLIMSIVSFCFIFLLLLFFRYILKRYFNYMLNYKVWYLTLL
                     AGLIPFIPIKFSLFKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAETIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLNVPFFN
                     KNILSHSFNGKKSLLKRRLINIKEANLKKQSKLILIFICIFTFLLMVIQSQFLMGQSI
                     TDYNYKKPLHNDYQILDKSKIFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPNSTYK
                     IYLAMFGLDRHIINDENSRMSWNHKHYPFDAWNKEQDLNTAMQNSVNWYFERISDQIP
                     KNYTATQLKQLNYGNKNLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGKPSGK
                     NAELISEKILKEMGVLNGQ"
     gene            14990..15835
                     /locus_tag="NBK22_000971"
     CDS             14990..15835
                     /locus_tag="NBK22_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaZ family penicillin-hydrolyzing class A
                     beta-lactamase PC1"
                     /protein_id="HCD2414169.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
     gene            16499..17668
                     /locus_tag="NBK22_000972"
     CDS             16499..17668
                     /locus_tag="NBK22_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCD2414170.1"
                     /translation="MSIRLNKKLNENAFNDPTKETIQKSINQLCSDVDSGQVIMPIFQ
                     RDLSWTAEKKIDLYNFQLGGFAPVSPISMNRIGPKSKGMPHVKLLSRTEIEELNEGSL
                     SVIDGQQRISTNYQAYSNDESIQEIALDLTKGKFVNLKEKKPSKNQIPVGVLYNKDPE
                     VYTEYLRFNPKLAEFSVSSILGQIRTKFFNYFYTINYAQDLSGEEQIEWFDVLNLAGS
                     RVPELQMKLTKLQIKGLDFYKEYSNIFRDRLEMAGLDHLFIQKNTEVSIPLATLNSAF
                     EIVSGKKNHTSNYSPIPSDAKGSFLNELEPDQLRKCFKMTLNGLEDALNFIDINSLRE
                     PSRIDYISYLSGYFTYNKNASNTSIQNVINWYNNTNFGNKSNQERRELYNELLMC"
     gene            complement(17850..17942)
                     /locus_tag="NBK22_000973"
     CDS             complement(17850..17942)
                     /locus_tag="NBK22_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD2414171.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
     gene            complement(18417..19025)
                     /locus_tag="NBK22_000974"
     CDS             complement(18417..19025)
                     /locus_tag="NBK22_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017432088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414172.1"
                     /translation="MKNLKSFFIKNTLVLTSTALLFSSFEPVVHAAENKEVVKTVEND
                     NVEFNELLEGQEYIWEVLSRDDEGFKMFLDEQRAFNPNFDNDVLLAKQEGALNSSVFA
                     NNSMTLNSNKPTPRGPIGATLKAIKALSPSLRHGGTAISWIIKPLSRKHAVLVKKYSR
                     KMSNAIDRLDKSTKSAVENAFVKAGIPRSDAKTLTYIVFLVI"
BASE COUNT         7141 a         2965 c         2588 g         6897 t
ORIGIN      
        1 aaaaaaaata atctctcaaa aagttttctc ggcataaaag tacctctaac ttttatacat
       61 aaagtcatga gcataaggga aagtgccacc acttttatat tttggcagac ccataacgtg
      121 gtgaactcgg cagtgccgag ttatttttcc tagaaaaaaa gtgacatcac ttttttaact
      181 catatgtata aaaaccataa cgttatgtat acatacaggt tacgtataca tatactttat
      241 gtatataaaa catacacaaa agacatgcat catacacatc aattttttta aaaatacatg
      301 tctttttgat atatattttt tttgcgtttt aagaggtcat ttagagcatt ctctaatttt
      361 gagtacaaaa atacgtaata taaaaaaatc gtccttttaa aaccttttta ggacatttta
      421 aacacatttt tatgatttgg atcaatgtag aataaatgtc ataaacgtat agaaaaaata
      481 cgtcgtcatt tctaagtaca gataaatttc tgatgcagta ctaaattatg gggaaaccta
      541 cctgtaaaaa ataaaaagat tactatattt taattttatt ttttctgttc gttttttcga
      601 caccaggacg ggcgattttt acgtactgcc catttacatg caaatttaaa aattagcaat
      661 aaatcgttcc gaatttgcat tttaaatggg caaacgtgcc gtttgatcac atagttcgtg
      721 tagcgacaaa aagagtataa cgaaagtata ataagcgtat aaaaaaagtg taatgataga
      781 aaatagcatt atactttttt agtcgttcat cgctcatcag aacaacaaat aaatttttaa
      841 gaaataaatt ttgagagttg accacccgcg ttctccgtaa actacaaatg tgggtggtca
      901 actctcaaaa tatttttata tttttttatt gttcttcttc ggtaagttcg tgaagttcac
      961 ttgccaggta ctctttctga ttccaaatat aataaatttg gtaaaggtat tcagtgggat
     1021 cgatttcttg gttctgttgc aaagttaaaa aataagtgtc taaccttatg tttaaaataa
     1081 tacttcgtat taactagcta gcatgatgct aattacacgg catggcgaaa acccgtagat
     1141 ctgaagagat ctgcggttct ttttatatag tccataaata cattcaatgc ctttgatcgt
     1201 attctttgcc gtattgatac tttgataact tattttcctt actttgatat gacgatgatc
     1261 ttgttcaatg agattattta aatatttaga ggtacaatga cagttggggg taagtttaaa
     1321 atctttaatc actttggaca ttgccacctt cgttgaagga gcttggtcta taattatcat
     1381 ttgaggtttg ccaaattgtt taataagacg tttaataaac gtatatgctg agtgattatc
     1441 tcgtttctta cgtaacgaaa tatctagtgt atggccatct gcatcaatag cgcgatataa
     1501 ataatgccat tgccctttaa ttttgatata cgtctcgtct atatgtcatt tataataagc
     1561 ttgcttattc tttttcttcc aaatttgata caaaatagga gcatattcct gtacccatcg
     1621 ataaattgtt gaatgatgaa catagatgcc tcgttctctc aatatttcag atatatcacg
     1681 ataacttaat gcatatctta gatagtagcc aacggctaca gtaatgacat ctttgtcaaa
     1741 ttgcttatat ctgaaataat tcatcctctg cacctcattt ttgttgacta ttatactata
     1801 tttctaactt tgcaacagaa ccttcttttc tctccatggg cgctgtttcc catttttcat
     1861 catatctttt aatgttttat tatcttgatt ttcgtctata tatctagtag tattgtattg
     1921 catataaaaa gacccccagt attatttaag attcgacacc ttaataatat aactggggtt
     1981 ttttattgtc aaattttcct ttcacgcaca aaaacacgtt taaacgctgt aatatcaaca
     2041 gttacagctg tatatataaa atcgagttat ttctatatag tatcaagaca agaagaaact
     2101 cgtttcactc gtttcaaaaa ccctcatgat ttttagagaa aaactctctt atttcatcgt
     2161 ctttaataaa ataatatgcc atctttccat ctttataaaa atcaagtacc tcatttttat
     2221 ataaaagcct taaatgatgt gaagttgaag caacactcat tttcaatatc aaagaaatat
     2281 cacaaacaca caactcatct tctttaatca aagataatat gattttcaat ttcttctcat
     2341 cacaaatttt ttctaaaatg ttaagtaatt tcttagattt atcatcttct aaaaaactta
     2401 aagcattgtt aactttatcc tcatgtacac agatcacatc acaagtattt tcataactca
     2461 tataaaatca caccctaaat taaaatataa atcctaaaat tgtttgaata gtgtcatttt
     2521 caataataat aaataaacct aaagctatat aaatgatagc cataatccaa cgactaaatt
     2581 tctcaacaat ttctccaatt cctgggatat tagctaattt ttgtgcagta aacaccaaga
     2641 agaaaattaa aattaaaaag acaaacagag taagtaataa attagtaaca cttaatgtca
     2701 caaaatacgg aacaaataaa ccaatattat cggcgccaca acttgctatc gtaacaattg
     2761 caaccgtacc aactaattta gacaatcctt tttcattcaa ttctttttta gctctctttt
     2821 ctccttcaca atcatcataa atagccactt taattccaag ataaattggt attaaaccca
     2881 ataaacctaa tatccacttt tctggaacat aatttaatac aaaagctaaa aacaaactaa
     2941 ctaatattaa aataatagac cctaaatatt gaccaacata aatatctcta tattcttttc
     3001 tagtctttgc tctagcaaaa aatattaata aaattactaa taaatcaact gctgtagcaa
     3061 tataaagaac agcagcagca acaaccgttt gaatcataat acacctcatt caaaaatgtt
     3121 tttgaatgta ttataactta tatttttaaa taatcatagg tgctttattc caattgcttt
     3181 attgacgttg agcctcggaa cccttaacaa tcccaaaact tgtcgaatgg tcggcttaat
     3241 aactcacgct atgccgacat tcgtcttcaa gtttagttaa gggttcttct caacatcaat
     3301 aaattttctc ggcataaatg cgtcgtctat ttttttatct tcatgataat catatttttc
     3361 ttccatctcg tcaaaatcat catcttcata gaaaaacaaa aagtaagcaa caacaccaat
     3421 aggaataaaa aatagtgtga aaacaagaag aaactcgttt caaaatcccc tcaaatattc
     3481 taattattct ttttgaaatt cataaattta acaaccaaat aaatagcatt tattcccatc
     3541 aaaataacat acggaattaa tatatataaa ttttccaaat gagatataaa aattaatcca
     3601 agtaaaaaga aaacatttaa aatagttgct ataacgtaac ctttatctaa cactgtgtct
     3661 ttattcaaaa taaaaactcc tttttaaaaa actttatcac aagacttact caaattataa
     3721 atacctttat tttgaatttt aagggggtat tttaaagatt taggggtaaa tcatatgttt
     3781 ttctatctaa aacctacata atctcttaaa acgcaaatat gagccaaata catatatgct
     3841 attttcaaaa acaaaatttt aaataaaacc aatgactatt ttttaaacac tgcccattta
     3901 catgcaaatt aaaagttagc ataaaatcct ttaaaacttc ccccatgttt ttctaaattc
     3961 ctgcaagaca ttttttatca aaatcgagaa tttttatttt ttctctaaga actccgtaag
     4021 agcataaggg aaatttgacc ccatgtatac atggggtatc tggcaaagcc agacaattca
     4081 aagcttcaac aatattttta tgtggggtca taaaaagctt aagtgtcaca cttttgtgac
     4141 acaatatatt gcatttgtca caaaaataag acagtacttt tttataaaaa aacagtaaaa
     4201 aaagacatca gtcatcacta atgtcttaaa ctctttaatt atttataaat aatcattacc
     4261 ccaaaataaa atacagaact tgaaaactta atatcaatga cttcgatttg gttatcatta
     4321 ataaaattat tgatttttcg ttctaaagaa cttccagtca ttccagttaa aattttagta
     4381 ttcatatttt taactcttta aattttttaa ataataattt tgcttttcga aattccaaat
     4441 tgctgtaatc gaatgtgctt tttcttcttc gtctgtcgtt ttatcttcat cacttgtatt
     4501 aatcaaatta ccatcttcgg catcgtctaa gtttaaaatt ttatgctttt ctttaagtaa
     4561 tccaccataa cttaacatac gttttcgata caaacctttt ctagatcact taaaatttct
     4621 tgattttttt cgtcgtcatc agttaaaaag tcagatgatt taacggaata ttttgatgtc
     4681 tctttgattg ttataaaatc atttgaatta ttgattaaag atattatatt gtttcatgtt
     4741 tttacggcgc atataaaggg catgtgtttc ttcaagttca gctttaatgg attgcattaa
     4801 ataaccttgc atagcttcaa cggtttcatt cttttgtagc aacatcgctt taaaacgttt
     4861 caatacactt acaatttcaa actcaacatc ttctaaacga taataagtat catgtgaagt
     4921 attaaatgac tttttccctt tgagtaatac gcttttaata atccgaattt ctctaatttc
     4981 aaatttactt aaatagtctt gaagttgttg tggcaattct tcgagtactt ggaacttaag
     5041 ggcgtcatta ttaaattcag ttttttgatg atttgtatga ttcgaatgat gattattatt
     5101 tattaaagta ttagatataa tatcattcat atcatacgta tcattatcct cattttctat
     5161 ataattagta ttatttaact cagtatcatt agactgtgaa tttggaagtt cttgaccact
     5221 taaattcaca gttctagaac ttccaaattc attgttctgg aactgtgaat ttggaagttc
     5281 cttggtaaga cttgcttcaa tatcataatt tggttttaat aaatataatc tatttggttt
     5341 attaaggcca acacgctctt gagttaataa attaaaattt actaactctt ttttcaattt
     5401 tatgattttt tgttctccat aacctaagta ctcgcacaaa tgtttattac tgaaaataaa
     5461 gtaaatgtta ttatttctat ctacccattc attttggatg gataattcga aacgatcttt
     5521 taataatgca tacataattt tggcttctgc tgacattttc ttaaaatatg catcttcgaa
     5581 caaatattta ggaagttggt aaaatctttc agtttttact tgtgataaat tgtatttctc
     5641 aaaattgggc ataataaaaa cctcatttcc actttttgga tagaggtaat aaaacaattt
     5701 ttacgtaggt tatatgtttt gtacttgcac ttatatgatt agatatatat aatacaagta
     5761 tcaaaaaatt atataaacag acgaatgcga atcgttttat taccgagagt cgatgctacg
     5821 ccaatagtat cgactctttt aatattcaca aagtaatatt aacactttta agattgtata
     5881 taaagctttt taagtttagt tgaaagaaca ttgtaacttt aaaatgataa aaattcacta
     5941 ctttagtacc taattagccg ttaaaacctt ctataagtga ctaattaggt actaaagtag
     6001 tgaaataggt agtaaattta gatgtcgaaa gatgaaaaaa tatttgttag gtggtaaaat
     6061 ttaggtacca atttatgtac ctaaggatga atgttatgaa aactattaaa atggttgctg
     6121 atgaattgaa tgtaactaaa caaactattg ttaataatgc taaaaactta aatatatctt
     6181 ttaaaaaaga aaatggaatt aattatatta atgataatga ttgtttaaaa attatagaaa
     6241 agatcactaa gaaagaaagg acaatgcaaa ataaagaatc aataaaaaaa gaaagattta
     6301 atgaaaatac tgcagagttt gaaaagcata tatacaataa ttcaaatggt tttgagtcat
     6361 taaaaacaaa agtaaatgaa ttagaaaagc aaatagaaat ctttgaaact agagctaaaa
     6421 atgatgaaaa atatattgag aatttaacta aacaactaga tcaacaaaat agcaatgtta
     6481 atacattaaa taaactgttg gagaatcaac aaatattagc tttagaaagt aataaaaaga
     6541 ttcaaaaact agaaaaccaa ttagaagaag aaagacagct taattactct tttgatacag
     6601 cgactaatga tagacaaagc attaatccgc aagaagcaac ttttactgaa gaatatcaaa
     6661 atagtaacca acaacaaaag aaaatacaac caatagaagc agaattaaat cataagggca
     6721 taactaaagc gaaaaaggat gacagtgcaa acgaagacgt attaaagaga gatgataaac
     6781 tatctgaaga aatagagagt ggacctgagg acagggaagt agaaacacct aaaaaggggt
     6841 tttggagtcg tttgtttggt agctaaaata attaattagg cactaaaagt tataaacttt
     6901 tagtgccttt tatatagtga gaacttctgg taatcattgg ttatgtaaac agagtgaatg
     6961 aggcaatcat agtccataca gaacctttat ttggctctca tttgtgtttt aagagcttat
     7021 gtaaaaaaac atataaaata ccccttaaaa tgcaaaataa ggatattagc ttatcacata
     7081 tgagttttgg ttttagtacg acactttttt aggtgtgtaa gtgcgccttt ggatataaaa
     7141 ataaaagcca acccagacaa agtggattgg ctttttcttt cgtacggtct gtttattttt
     7201 ccttcgacgc cgtatcgtcg atgtaaataa aaaaagttat gggctaaaaa tctacatcac
     7261 tttttcaaga atgtagtagc gatattcgac acaaattaat ttttataact gctcggaata
     7321 tctcaagccg tttctttgaa tttaaaatta aaaatcgacg caggtcgatg acggatctac
     7381 actagcgttt ggaacaaacg tataagtgcg cccttacggg agttaagtag attataacac
     7441 tggttagtag aggaatgcaa aattataaag tgtgttgagt tagattagac atttttcttt
     7501 ttaaagacaa atactacgat gattaagaaa ataataatat aaacaatatt accaataaat
     7561 aattcatgca gttctaattt tgtgaatttt ttcatgtttt tatcaacaat ttgaaccata
     7621 atattcatca tactaatagg gttccatttt aaccattccc atttatctac aacaattgtt
     7681 aatatccctg acaaaattga agtggcaaaa tacaatacaa taccgacagc aatagagaca
     7741 ccgggacttt tcattgcaca tgaaattaat aaggtgatac ttaagactaa ccatgttcca
     7801 acatatgtac ctagtccagt taataacatt ttacttaata acgataacgc atcaccctta
     7861 ctttcaagga gattgatatc gttgaagaaa atagcccata gaataattcc tacaacaata
     7921 ctaacaataa aatacagtag tgaaaaaagt attaatgtaa taattttact aataataatg
     7981 cttgttctcg tatattttcg atagagaaga tttttaattg taccatgata aaactccatt
     8041 gaaataatgg tgctggcttg aataattaat aaaaagataa accatgaaaa accactaaat
     8101 gctgattcaa tagatgattg tggagaaaaa acatcatcat agtttttaga tattatagct
     8161 tgaacaacca ttaataacat aatgataatt ggtataataa acgtcgattt ctttttaaac
     8221 aatttaaaac attcttgctt aattaaagtg cccattttac gattgatcct ccttatcaat
     8281 gatacttaat aatacatttt ctaaatcgtt atctttatat tggtttaatt cttcttcggt
     8341 agtttcagta acaattttac ctttgttaat gataaggata gaattggtga tttttactaa
     8401 ctcacttaat atatggctag aaattaaaaa ggtaacgccc tcttccgctt tttttacaat
     8461 gagttctcta acatctctta cagccctagg atctaaccca ttcataggtt catctaaaat
     8521 aattaattgg ggttgattta agaaagctat tgcaatacct aatttttgtt tcatacccaa
     8581 ggaatatgtt ttggctttat tatgtatata ctcatccatt ttaagttgag agacaattgt
     8641 atcaatatct tgagtatttt tagtttcatt tagtagttta agattgtcat agcctgacat
     8701 aaagggatat actcctggat tttcgatcaa tgcaccaacg ttactttttt tatgcttatt
     8761 ttcaataact tcaaagttac cttcttgata aatcgagtag cctaatataa ctttcatcaa
     8821 agatgttttt cctgccccat ttgctccaac aagaccgaca atctgtcctc gttttaaatt
     8881 taaagaaata tctttgagaa tagtcttatt acctatcttt tttgttaaat gttctactgt
     8941 taaaacatcc atacaattat ctccttttta attatttaga tatattttaa atccaaactt
     9001 aatgtatcat aaaaaaataa attatttaaa tacatgttga gatacataaa tataaattaa
     9061 atgaagtgct gcattttata agtacactct aaattaacaa gatgtataat caatctcaaa
     9121 ttgtaatttt atacttttat tatatgatct ttgtacttag agatatattg taaaaaaacc
     9181 aataaaatgt tatattaaca ttactatttt atcgatgaag gaggattaaa atgacagctt
     9241 ctatgagact caaataaacc tttttgaata caaaactaaa ttaattcaaa aaggcttgtc
     9301 cgcaatacac acactcatag gatgacttat gtcatttttt atccttcgat aggaataata
     9361 aacgctttca aatactaaaa catcgtaata attctttaag tgtttgaaag tgtaatgtga
     9421 atgagacggg agtagcctgt ctcatttttt gcgtacacgt cctttaaata tacaaggagt
     9481 gtataaatat ggaacaatat acaattaaat ttaaccaaat caatcataaa ttgacagatt
     9541 tacgatcact taacatcgat catctttatg cttaccaatt tgaaaaaata gcacttattg
     9601 ggggtaatgg tactggcaaa accacattac taaatatgat tgctcaaaaa acaaaaccag
     9661 aatctggaac agttgaaacg aatggcgaaa ttcaatattt tgaacagctt aacatggatg
     9721 tggaaaatga ttttaacacg ttagacggta gtttaatgag tgaactccat atacctatgc
     9781 atacaaccga cagtatgagt ggtggtgaaa aagcaaaata taaattagct aatgtcatat
     9841 caaattatag tccgatatta cttttagatg aacctacaaa tcacttggat aaaattggta
     9901 aagattatct gaataatatt ttaaaatatt actatggtac tttaattata gtaagtcacg
     9961 atagagcact tatagaccaa attgctgaca caatttggga tatacaagaa gatggcacaa
    10021 taagagtgtt taaaggtaat tacacacagt atcaaaatca atatgaacaa gaacagttag
    10081 aacaacaacg taaatatgaa cagtatataa gtgaaaaaca aagattgtcc caagccagta
    10141 aagctaaacg aaatcaagcg caacaaatgg cacaagcatc atcaaaacaa aaaaataaaa
    10201 gtatagcacc agatcgttta agtgcatcaa aacaaaaagg cacggttgag aaggctgctc
    10261 aaaaacaagc taagcatatt gaaaaaagaa tggaacattt ggaagaagtt gaaaaaccac
    10321 aaagttatca tgaattcaat tttccacaaa ataaaattta tgatatccat aataattatc
    10381 caatcattgc acaaaatcta acattggtta aaggaagtca aaaactgcta acacaagtac
    10441 gattccaaat accatatggc aaaaatatag cgctcgtagg tgcaaatggt gtaggtaaga
    10501 caactttact tgaagctatt taccaccaaa tagagggaat tgattgttct cctaaagtgc
    10561 aaatggcata ctatcgtcaa cttgcttatg aagacatgcg tgacgtttca ttattgcaat
    10621 atttaatgga tgaaacggat tcatcagaat cattcagtag agctatttta aataacttgg
    10681 gtttaaatga agcacttgag cgttcttgta atgttttgag tggtggggaa agaacgaaat
    10741 tatcgttagc agtattattt tcaacgaaag cgaatatgtt aattttggat gaaccaacta
    10801 attttttaga tattaaaaca ttagaagcat tagaaatgtt tatgaataaa tatcctggaa
    10861 tcattttgtt tacatcacat gatacaaggt ttgttaaaca tgtatcagat aaaaaatggg
    10921 aattaacagg acaatctatt catgatataa cttaaaaact atacactaat ttatattgaa
    10981 tatttcttta aagtattata ataaagtaaa taacaatagt accataaccg atcatttttg
    11041 agaaaaagaa tatagaagaa aagagaggga gtttttaccc tctcttttct tgttttatta
    11101 ttaaaatctc ataatagatg attaatatgc gttttagaac gctgtgagaa acgctcattt
    11161 gcattgacaa cctatttatc aagataatcc cttatcgtgt ttaattctat aaacagtctg
    11221 ccttgttata tttacttctt ttgcaatttt actaattgct tggccttctt ctaacatttc
    11281 gacaactcga tgatagataa cacgcttttg aggatctttc gcgtttggcg aataaagtaa
    11341 aggtcgtcct ttataaacgc ctttttcttt tgcaacttga attccttgtg cttgtcgacg
    11401 tttactttcg tttctctctt gttctgaaac cattgctaaa atttgaacaa ttaaatcttt
    11461 cataaattta tccagtaatg gattactaat aacttcattc atcataggta aacttgtaat
    11521 cattaactga acgtcttttt cttttaagta attcacagtt tcaataattt catcataatt
    11581 acgtcctaaa cgatcaatag attccacaac gaatcgatct cccgttctta caaagttaag
    11641 tgcttcttga aatactggtc tattttcaat agatttccca gactgttttt ctgtaaatat
    11701 tttttcagcc ccaaacgtct ttaaattttc aagttgtctt tctaaatttt gatcgatcga
    11761 tgatactcac gcatagccaa caatcattga ttttcacctc ataattaata tacaccctaa
    11821 ccatacattt tagttgctat aaattcaaca attatacatg tatgattagg gtgtatcctg
    11881 tctaattttt atctttctta aaatttagta atattattta tctttatttt cttggttatt
    11941 taacttattg agataacgat agattgtggt acgtgaaact ttccattgtt ctgcaatagt
    12001 tttaattggt gttccattat cataaagtgt tttgagtaat tttaaatctt gtttatttaa
    12061 tttttcgggt cgacctccat agcgtcctct agcacgagct gcaattcgcc ccgctgaaga
    12121 acgttctaaa attaaattac gttcaaattc tgcaaatgct gcgaataagt gaaataacaa
    12181 ttgtcctgta gaacttgact tatccattgt tatattttct tctaaactat gaaaactcac
    12241 gcctcgatta ttaagttcat taactaatgt gattaaatct gccatgttac gtcctagtct
    12301 atctagtctc caaacaacaa tcgtatctcc tgacctcgca aattcaatgg ctctatctaa
    12361 gccaggacgt ttactttttg ctccacttat gtgatcgcta aatatttttt cacaaccata
    12421 ttgatttaat cgatcttcct gcaaattcaa attttgcaaa ccagttgaaa ctcttgcata
    12481 acctattttc aaaatacaaa cctcctatat acaccttcag tgtaccataa cttaatgaca
    12541 aggtcttttg tgaacataga ttatttgttg ggtttttgaa cacataattc tatgtaatac
    12601 atacttattt agacataaaa aacgagtgta cagaaaatgg ttattttctg tacactctca
    12661 tctttaataa tatattttaa atgttattaa caaaactaat ttaataagag tcaagcatag
    12721 tttacatatg tattatgttt gaaacatatc tctaattact ttttactaat atcatttaaa
    12781 atgtctcgca attcttcaat ttctttgtta tttaattctt catttttcgc aaaattcagc
    12841 actaaacttt tcatgtcccc tccatacagt ttattaagaa aggttttagc agttttcatt
    12901 ttaatatcgt cttctttaat atttgatgag taaaaataaa tattctctga tttgtatcgt
    12961 tttataatct cttttttata tagtcttgtg attaatgttc taatcgtttt atcgctaact
    13021 tctttatatt tttgaatttc aactacaatt tcattagctg atactgattt tttaccccat
    13081 attatattca taacatccca ttcagccata gatatttcaa cttgcttatt ggccatttaa
    13141 aacacccatt tcttttaata ttttttcact gattaattca gcatttttcc cagatggctt
    13201 tccatctgat aaatgtgtag caaaataata cttatcatga tttgtaatta cgtaacctac
    13261 aaaccaccca ttattatact tcccgtttac tatacctgta cctgttttcc catacagttc
    13321 atacttttca tttttcttaa tcaataatga agaagataat tgattctttg cttttttact
    13381 aaaatggtta ttttgttcca tcatattttt aaaaactatt acttgttcaa gattagatat
    13441 tttcaaacta tcttccatcc aatagctttt ataacttccc aaatttttat taccataatt
    13501 taattgcttg agttgagtcg cagtatagtt ctttggtatt tgatcgctaa tacgttcgaa
    13561 gtaccaatta actgaatttt gcattgctgt atttaaatct tgttccttat tccaagcatc
    13621 aaaagggtaa tgcttatgat tccatgacat acgtgaattt tcatcattta taatatgtcg
    13681 gtcaagtcca aacatagcta aataaatttt ataagttgaa ttaggagaat acctttttct
    13741 actttctttt tcgttataaa tatagtattt gtctttcttc atgctgtaca tgacgaaaga
    13801 tccactattt gaaccaaaaa ttttactttt atctaaaatt tggtaatcgt tgtgtaaggg
    13861 ctttttataa ttataatcag ttatggattg ccccatcaaa aactggcttt gaattaccat
    13921 gagcaaaaaa gtaaaaatac aaataaaaat tagaattaat ttggactgtt ttttcagatt
    13981 ggcttctttt atattaatta atcttctttt gagtaatgac tttttaccat taaatgaatg
    14041 gcttaatata tttttattaa aaaatggaac atttaatacg gagtccataa tagattccgc
    14101 ataagtttta aattcatttt tgttaatatt attaaggaca aatctatcgg cttctacttc
    14161 attgtcatga attatctttc tttttacaat atgtactaat ggattgtaac tcataataat
    14221 agagaagata ttaaagataa ttaaatgtaa agtatctcta tttttagcat gagcatattc
    14281 atgcaatatt atatatttta gtcttttgtc aattacactt ttaaaatatg aactaggaat
    14341 caaaataata tatttcccat accaaaaagt tattggagat tgaatagtct ctgcttttcg
    14401 aatcacaata ttttttttat attgatggtt gaaaagtatc gtatctattt tatttttttc
    14461 attttcgttt agataaagtg actgtttctt taaatattta agatataata aggctttcaa
    14521 aaatttaaaa cttaaaataa taactaaaac tatccaaata actgtgcaga tattatcaat
    14581 tgaatcccaa ttaaacttat ggatatctgt tgtgaactct tgaataggtt tggtggtatt
    14641 tatgttatgg ttcaagtcgt gtgacttact ttcaactgtg ggcgcttgat tattcacatt
    14701 attaaattta aaaagagaga atttaatagg aatgaaagga attaatcctg caagaagagt
    14761 tagataccaa actttataat ttaacatata attaaaatag cgttttaata tgtacctaaa
    14821 aaataataac aatagaaata taaaacaaaa gcttactatg ctcattatta ataacttagc
    14881 catttcaaca ccttctttca agtatttata ataaactatt gacaccgata ttacaattgt
    14941 aatattattg atatataaaa attacaactg taatatcgga ggatttattt tgaaaaagtt
    15001 aatattttta attgtaattg ctttagtttt aagtgcatgt aattcaaaca gttcacatgc
    15061 caaagagtta aatgatttag aaaaaaaata taatgctcat attggtgttt atgctttaga
    15121 tactaaaagt ggtaaggaag taaaatttaa ttcagataag agatttgcct atgcttcaac
    15181 ttcaaaagcg ataaatagtg ctattttgtt agaacaagta ccttataata agttaaataa
    15241 aaaagtacat attaacaaag atgatatagt tgcttattct cctattttag aaaaatatgt
    15301 aggaaaagat atcactttaa aagcacttat tgaggcttca atgacatata gtgataatac
    15361 agcaaacaat aaaattataa aagaaatcgg tggaatcaaa aaagttaaac aacgtctaaa
    15421 agaactagga gataaagtaa caaatccagt tagatatgag atagaattaa attactattc
    15481 accaaagagc aaaaaagata cttcaacacc tgctgctttc ggtaagactt taaataaact
    15541 tatcgcaaat ggaaaattaa gcaaagaaaa caaaaaattc ttacttgatt taatgttaaa
    15601 taataaaagc ggagatactt taattaaaga cggtgttcca aaagactata aggttgctga
    15661 taaaagtggt caagcaataa catatgcttc tagaaatgat gttgcttttg tttatcctaa
    15721 gggccaatct gaacctattg ttttagtcat ttttacgaat aaagacaata aaagtgataa
    15781 gccaaatgat aagttgataa gtgaaaccgc caagagtgta atgaaggaat tttaatattc
    15841 taaatgcata ataaatactg ataacatctt atattttgta ttatattttg tattatcgtt
    15901 gacatgtata attttgatat caaaaactga ttttccctct attattttcg agatttattt
    15961 tcttaattct ctttaacaaa ctagaaatat tgtatataca aaaaatcata aataatagat
    16021 gaatagttta attataggtg ttcatcaatc gaaaaagcaa cgtatcttat ttaaagtgcg
    16081 ttgctttttt ctcatttata aggttaaata attctcatat atcaagcaaa gtgacaagtt
    16141 gttataaatt caacaacttc atttgtatga ttagggtgta cactaaacgt acaattccaa
    16201 taaaaagtaa aaatatgaag aaaagattag aagcagcgca aataagagaa agaaaagaga
    16261 gacgatcatc attcaaaaaa tatcaaatag attaggataa gtcgttcgac attataaagt
    16321 cataaaacca atataaataa tgctataaaa atacaagcag cttaaattag ctgcttgtat
    16381 ttttatagca ttatttattt atatttcgct atttataaaa cgctgctaaa aaataattga
    16441 ctttttgacg tcttagattt aacgttatat taacaagcag caaaaggagt tattgagtat
    16501 gtcaattaga ttaaataaaa aattaaatga aaatgcattc aatgatccta ccaaagaaac
    16561 tatccaaaaa tcaattaacc aattatgttc tgatgtggat tcaggtcaag ttattatgcc
    16621 aatctttcag cgcgatttat cttggacagc cgaaaagaaa atagacttat acaattttca
    16681 attaggaggt tttgctccgg tttctcctat ttcaatgaat agaattggtc ctaaaagtaa
    16741 ggggatgccc cacgtaaagc tattaagtag aactgaaata gaagaattaa atgaaggtag
    16801 tctaagtgtg atagatgggc aacaaagaat ttcaacgaat tatcaagctt attcaaatga
    16861 tgaatcaata caagaaatag cgctagattt aactaaaggg aaatttgtca atttaaaaga
    16921 aaaaaagcca agtaaaaatc aaatacctgt tggtgtttta tacaataaag atcctgaggt
    16981 ctatactgag tatcttagat tcaatccgaa attagcagaa ttctctgtat catctattct
    17041 aggacaaata cgcaccaaat tttttaatta tttttatacc attaactatg ctcaagactt
    17101 atctggtgaa gaacaaattg aatggtttga cgttctaaat ttggcgggta gtagagttcc
    17161 tgagcttcaa atgaagctca ctaaattaca aataaaaggc ttggattttt ataaagaata
    17221 ctccaacatt tttagagata gattggaaat ggccggactt gaccatctat ttatacaaaa
    17281 aaatacagag gtctctatac ctttagccac actcaattct gcttttgaaa tagtgagtgg
    17341 taaaaaaaat catacttcaa attacagtcc catcccgtca gatgccaaag gatctttttt
    17401 aaatgagtta gagcccgatc aattaagaaa gtgttttaaa atgacattaa acggcttaga
    17461 agatgccttg aactttattg atatcaatag tctaagagaa ccatcaagaa ttgattatat
    17521 ttcctattta tctgggtatt tcacctataa taagaatgct tctaacactt ctatacaaaa
    17581 cgttataaat tggtataata atactaactt tggaaataaa tccaatcaag aaagacgaga
    17641 attgtataat gaattattaa tgtgttaaag ctcaacaacc acagtaaccc tccatagttt
    17701 tctgtcgcaa acagtagttt ttgcatttct ctactaacca gtgttataat ttatataacg
    17761 aaaatgatta acaattaatg caccaatccc cacactattg cagtagtgtg gggatttttt
    17821 cgatgctggc ttataaagtc gcctattaat tatttgttgc gtttactaag ccaataagta
    17881 taatacgcaa cagcacagcc actgatgact ggtgctatga tgtgaacgaa aatgattagc
    17941 aaagtaatgc acctcctctc ttcgtcaatt gacgcctgag agataggcga ctctattatt
    18001 ataccatctg tgaatataca agcatatcta cttccgataa gtcgtcttat gttatataac
    18061 ttcgtttttt atagtatcaa gacaaaagaa gaaacgcagt tcaaaatcat cacctaaatt
    18121 cattaatcta aagaaaaaac ggtgtaataa atagtgaata ttatagtcta ttaaagaata
    18181 gatttaaaaa cactctaaaa ggtgcattaa agatttgtaa aactttaata tatccttttt
    18241 aggtgaaaac ttatgataat tgtaggttct ctcattatct atgattatta attttaactg
    18301 tatctagaaa atcatcaccc caatcacaaa ttgaaaatac tactttatca agataagaaa
    18361 aaagaaaaaa cttgccctaa gattttataa aatcttaggg caagtcagct caatatctaa
    18421 ataactaaaa atacaatata agttaaagtt ttcgcatcac ttcttggtat acctgcttta
    18481 acaaaagcat tttcaacagc tgatttcgta gacttatcta acctatcaat tgcattagac
    18541 atctttcttg aatatttttt tactagaaca gcatgttttc tacttaatgg tttaatgatc
    18601 caagatatag cagttccacc atgtctcaaa gaaggtgata gagccttaat agcttttaaa
    18661 gttgcaccga taggacctct aggagtaggc ttatttgagt ttaaagtcat agaattatta
    18721 gcaaatactg aagaatttaa agcaccttct tgttttgcta gtaaaacatc attatcaaaa
    18781 ttaggattaa aagctctttg ctcatctaga aacattttaa atccttcatc atctctagat
    18841 aaaacttccc aaatatattc ttgtccttct aacaattcat tgaattctac attatcattt
    18901 tctactgttt taacaacttc cttattttct gcagcatgaa ctacaggttc aaaactagaa
    18961 aataataatg cagtagatgt tagtaccaaa gtatttttaa taaaaaaact ttttaaattc
    19021 tttatatgac ttttatcctc caattataaa tgttaaaatt ttttattatt aaatattata
    19081 tacataaaag gaattaaatt cattatgaaa aaagtaaaca aagagtactt attgaacttt
    19141 ataattgtaa cgttcttact cggaataata atcataccta tatccgtatt agtattctca
    19201 ataccatttt ttagcataat aggtttccaa tcattagcag ataatttaaa tataagttct
    19261 ttaataacaa taaattggta ttcaaataat atgttttata acattggcta tattgcatta
    19321 ttctttatag taacttatct aatatttatt tttcttgaac tttttataat atttttacat
    19381 ataaaataca aaaagaaatt taataatatt atcttattca tattctttat aactgctaat
    19441 atgatcgtat taaacttaat aattttgaag attttctaca acattcatat aaacttatat
    19501 tttcaaattt tcatttattc tttatgcttc tatcttttat ttactcataa agagatttac
    19561 aaaatatttg aagaaaataa aagtaaaaaa t
//
LOCUS       DAHHYJ010000036        19521 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000036 DAHHYJ010000000
VERSION     DAHHYJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19521
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.036"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(226..2634)
                     /gene="priA"
                     /locus_tag="NBK22_000975"
     CDS             complement(226..2634)
                     /gene="priA"
                     /locus_tag="NBK22_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCD2414173.1"
                     /translation="MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
     gene            complement(2634..3833)
                     /gene="coaBC"
                     /locus_tag="NBK22_000976"
     CDS             complement(2634..3833)
                     /gene="coaBC"
                     /locus_tag="NBK22_000976"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCD2414174.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            complement(4049..4267)
                     /locus_tag="NBK22_000977"
     CDS             complement(4049..4267)
                     /locus_tag="NBK22_000977"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCD2414175.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            complement(4267..4890)
                     /gene="gmk"
                     /locus_tag="NBK22_000978"
     CDS             complement(4267..4890)
                     /gene="gmk"
                     /locus_tag="NBK22_000978"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCD2414176.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLEHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            5165..6862
                     /locus_tag="NBK22_000979"
     CDS             5165..6862
                     /locus_tag="NBK22_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCD2414177.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKINPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVSRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            complement(7125..7526)
                     /locus_tag="NBK22_000980"
     CDS             complement(7125..7526)
                     /locus_tag="NBK22_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2414178.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(7963..8175)
                     /locus_tag="NBK22_000981"
     CDS             complement(7963..8175)
                     /locus_tag="NBK22_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414179.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            complement(8205..8816)
                     /locus_tag="NBK22_000982"
     CDS             complement(8205..8816)
                     /locus_tag="NBK22_000982"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCD2414180.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKLAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            complement(8816..9508)
                     /gene="pyrF"
                     /locus_tag="NBK22_000983"
     CDS             complement(8816..9508)
                     /gene="pyrF"
                     /locus_tag="NBK22_000983"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCD2414181.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQDTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQNDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            complement(9618..12791)
                     /gene="carB"
                     /locus_tag="NBK22_000984"
     CDS             complement(9618..12791)
                     /gene="carB"
                     /locus_tag="NBK22_000984"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCD2414182.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            complement(12784..13884)
                     /gene="carA"
                     /locus_tag="NBK22_000985"
     CDS             complement(12784..13884)
                     /gene="carA"
                     /locus_tag="NBK22_000985"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCD2414183.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            complement(13886..15160)
                     /locus_tag="NBK22_000986"
     CDS             complement(13886..15160)
                     /locus_tag="NBK22_000986"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCD2414184.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            complement(15178..16059)
                     /locus_tag="NBK22_000987"
     CDS             complement(15178..16059)
                     /locus_tag="NBK22_000987"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCD2414185.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            complement(16087..17394)
                     /locus_tag="NBK22_000988"
     CDS             complement(16087..17394)
                     /locus_tag="NBK22_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCD2414186.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            complement(17612..18139)
                     /gene="pyrR"
                     /locus_tag="NBK22_000989"
     CDS             complement(17612..18139)
                     /gene="pyrR"
                     /locus_tag="NBK22_000989"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCD2414187.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            complement(18539..19456)
                     /locus_tag="NBK22_000990"
     CDS             complement(18539..19456)
                     /locus_tag="NBK22_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD2414188.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNNDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            complement(19456..>19521)
                     /locus_tag="NBK22_000991"
     CDS             complement(19456..>19521)
                     /locus_tag="NBK22_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCD2414189.1"
                     /translation="VILIIIALLKDTSNKKEKEVK"
BASE COUNT         6048 a         3709 c         2859 g         6905 t
ORIGIN      
        1 tggcgcttgc attaaaatca gaattcgtaa atgaaaatac taataaattc cttatcaaac
       61 caaaatcacg taagttataa agccatataa aaagacagaa tataaactgc taatttaaat
      121 agttaacagt tgtatattct gccctttttc tgtacttaaa gtaaatattc aaacactggt
      181 tcaaatactc gtacatactg aacttgtttc taataattag taatgttaca tcatcatctg
      241 tggatcaata tcaatcttca atgctaattt ttcttttata aatttttcat ggtaatagtc
      301 atctaaaaac tgaatggctt gtaataatcc aggttcactt ttatatttca ctaaaatttg
      361 gaatctaaat tcattgttga ttctcgcaag tgctgccgga gatggaccta gtacaagcgc
      421 tttttctgtt aaatgctgta ataaaatttt atgaacatgc tgcgatgcta ccataacctt
      481 cttcatttct ttatgtgaga ttgtgaaatt aatcaaataa taatacggtg gatactttcc
      541 taatttacga tattccattt cctgacgata aaatgttaaa taatcatttt tttgaacatc
      601 caatattgaa taatgatctg gattatacgt ttgaatgatg acttgacctg ccttttcatg
      661 acgaccagct ctaccagcca cttgcgttaa tagttgataa gtacgttcgc tcgcccgaaa
      721 atcaggtaaa tttaacattg tatctgcatt cagcacacca actaaagtaa tatttggata
      781 atctaatcct ttcgcaatca tctgagtacc tagtaaaatg tcaccgttac ctttttcgaa
      841 ttcagtcaat aacttttcat gtgcaccttt ctttgaggtt gtatctacat ccatcctaat
      901 tatgcgcgca tcttcaaatt cttgttgcaa tagttcttca actttctgag taccagtacc
      961 tacttgtcga atgtgttcac tctcacaatt tggacattga ttcggtggcg tctcttggta
     1021 accacaatag tgacatttta ataagtctgt cgttttatga tacgttaatg aaatatcaca
     1081 gtttggacat tgcggtacat atccacaatc ccgacataac ataaacgatg cataaccacg
     1141 tcgatttaaa aataaaacaa cttgttcctg tcgatctaat cttaattgta tggcttcacg
     1201 taaatctttt gaaaacattg accgattacc ttcactcaat tcttcacgca tgtctactat
     1261 atcaatttca ggtaaagctt gttggttcac tctgtttggt aatgatagca aatgataaac
     1321 gcctttttca gctcgtgcat aactttcaag acatggtgtt gcacttccta aaatgactgg
     1381 acagtgatga tattcacttc gccattgggc aatttctcta gcgtgatatc tcggataatc
     1441 ttcttgttta tatgtagatt catgttcttc atcaatgatg attaacccta aatttttgaa
     1501 aggtgcgaac acacttgacc ttgcaccaac acttactctc gcacgaccat ccctaatttt
     1561 ttgccactca tcataacgtt ccccattaga taagccagaa tgtaatacag caacgtcatc
     1621 accaaatcga cgtttgaagc gtaaaaccat ttgcggtgtt agagcgattt caggaactaa
     1681 catcatcgcc tgttttcctt ggcttaaaac atcttctatc gtttgtaaat atacttctgt
     1741 tttacctgat cccgtcacac catgaagtaa aaatgtttct tgctcttggc ttacaatttt
     1801 agctttaatc gcttcatatg ctttatattg gtcttctgtt aattgctgtt ttgattcttg
     1861 ttcgaaaaca cgatctttaa atgggtctct ttcaacaacc gcgtcatatt tttcaacata
     1921 accttttttg ataagtccat ctaaactgga ttttgaaaag cccatatcct caatatcagt
     1981 taaaaatatt gttttatgtt gttcttcaga caagtaagca tacaaatcgt attgtttaat
     2041 aactttctcc aacttagcta atacttcatc aggatgatac ccttcaatga cacgaacagc
     2101 acgcttggtt tttttagtta tattttgtgt gagaatcgtt ttttcttcaa cgatatcatc
     2161 ttttaacaac ttcataagca attgaatatc attatttttt tgcgcatctt tataataata
     2221 gtaaccatgc ttatcaaatt tttgtaataa agctgaaggt agctctatgt catctttcat
     2281 cttaaatgct tttttatact tcgctttaat agcactcgga agcatcactt ctagcataga
     2341 aatacgttta atgacatgag ttgaacccat ccactcactt aaagctatta attctgatgt
     2401 taattctggt tgtatatctt tcacttctat gatttttttt aacttcgaaa cgtcaagttg
     2461 tgcatcaggt tctgctgtta cttccattac ataaccttga atcgttcttg gtccaaaagg
     2521 tacaattaca cgcacaccag gttggatgac agattcgagt tgttcgggaa ttatataatc
     2581 aaatttatag tcaacgctct tcgacgcgac atcgactatg actttcgcta tcattattgc
     2641 cacctagttt ctagttcatc taaaatttgt gcagctaata ctactttttt tcctttcttg
     2701 atatttactt tttcattatt tttaaaatgc attgtcaatt cattatcatc agaactaaat
     2761 ccgatagaca tatccccaac attatttgaa ataatcacat ctgcattttt cttgcgtaat
     2821 ttttgttgtg cataattttc aatatcttca gtctctgctg caaagcctat taaatactgt
     2881 gatgttttat gttcacctaa atatttaaga atgtctttag tacgtttaaa agatactgac
     2941 aaatcaccat cctgcttttt catcttatgt tctaatacat caaccggtgt atagtcagat
     3001 acggctgctg cttttacaac aatatcttgt tcgtcaaatc ggcttgtcac ttgttcaaac
     3061 atttcttcag cactttgaac atgaataact tcaatatctt ttggatcctc tagtgttgta
     3121 ggaccagcaa ctaacgtcac gatagctcct cgatttcgca atgcttcagc tattgcatag
     3181 cccatttttc cagaagaacg attggataca aatctgactg gatcgataac ttcaatagtt
     3241 ggtcctgctg taaccaatgc gcgtttatct tgaaatgaac tattagctaa acgattacta
     3301 ttttgaaaat gagcatcaat tacagaaacg atttgaagcg gttcttccat acgtccttta
     3361 gcaacataac cacatgctag aaatccgctt cctggttcga taaaatgata cccatcttct
     3421 tttaaaatat taatattttg ctgcgtacgt ttattttcat acatatgcac attcatagca
     3481 ggcgcaataa atttcggtgt ctctgttgct agcaacgttg atgtcaccaa atcatcagca
     3541 atacctacac tcaattttgc aattgtattt gccgttgcag gtgcaacaat gattgcatct
     3601 gcccaatcac ctaatgcaat atgctgtatt tctgaaggat tttcttctat aaaagtatct
     3661 gtataaacag catttcgact tattgcttga aatgctaatg gtgtcacaaa tttttgtgcg
     3721 tgattcgtta acataacgcg aacttcatac ccagattgtg ttaacttact tgtcaaatca
     3781 attgctttat atgccgcaat gccacctgta acggctaata atattttctt catattcaat
     3841 ctcccttaaa tatcactatg acatttacgc tttacatcat catatgcgca caaatgctca
     3901 ttactttttt atagatacaa atttagtatt attataacat caatcattgg ataaactaaa
     3961 aaaacacacc tacataggtg cgtttgattt ggatatgcct tgacgtattt gatgtacgtc
     4021 tagcttcaca tatttttaat ggtcgaaact attctttacc ataataatca cttgaaataa
     4081 cagggcgaat tttaccgtca gcaatttctt ctaacgctct accaactggt ttaaatgaat
     4141 gatattcact taataattca gtttcaggtt gttcatcaat ttcacgcgct cttttcgctg
     4201 cagttgttgc aattaaatac tttgatttaa tttgtgacgt taattggttt aaaggtggat
     4261 ttaacattat tttttagcct ccaaaatcat ttttctatac ttagcttcta cgcgctctct
     4321 ttttaagtgc tcagcttcta caatacattg aattctattc ttcgcaagtt ctacttcatc
     4381 attaactaca acgtaatcgt ataaattcat catttcaact tctttacgcg cttcgttaat
     4441 acgactttgt attttctcat cagattctgt tcctctacct actaatcgct ctctcaagtg
     4501 ttctaaactt ggaggtgcta agaaaataaa tagcgcatct ggaaatttct ttctaacttg
     4561 ctttgcacct tctacttcaa tttctaaaaa tacatcatga ccttcgtcca ttgtatcttt
     4621 aacatattga actggtgtac cataatagtt gcctacatat tcagcatatt ctataaattg
     4681 gtcatctttg attaaagctt caaacgcatc cctagtttta aaaaagtaat ctacgccatc
     4741 aacttcacct tcacgcattt gacgtgttgt cattgaaata gaatacttat atgatgtact
     4801 tggatcttca aatattcgtt ttctaacagt acctttacct actccagatg gtcctgataa
     4861 aacgattaac aatccttttt cattatccat gccttacgac ctctctaagc taatcttcta
     4921 ttatttaaat atgatatcac attgttcttt atattgtata gcatatttga aattgcatgc
     4981 cataatttct attaagtcta acaatatcgt tatattgcac gattaatttt aattaaataa
     5041 attgaattgc aaacttttag ataatgtaaa atgtatggca taatgtatgg ttcaataact
     5101 atactgaaaa gttacaatca tgttaaaatg aaacgaatga tatgaagaag gtggaagata
     5161 aattatggct tatgatggct tatttacaaa gaaaatggtt gagtctctac aatttttaac
     5221 aacaggacgc gttcacaaaa tcaatcaacc tgataatgac acgatactaa tggttgtacg
     5281 tcaaaataga caaaaccatc aattgttatt gtcaatccat ccaaactttt caagattaca
     5341 attgactact aaaaaatatg ataatccatt taatccaccc atgtttgcgc gtgtttttag
     5401 aaaacactta gaaggtggta ttatcgaatc gattaagcaa attggtaatg atcgtcgcat
     5461 tgaaatcgat ataaagagta aagatgaaat tggcgatact atttaccgca ctgtcatcct
     5521 tgagattatg ggtaaacata gtaacttaat tttagtagat gaaaatcgca aaataattga
     5581 aggatttaaa cacttaacac caaatacgaa tcactatcgt acagtaatgc caggatttaa
     5641 ttatgaagca ccacctactc agcacaaaat aaatccgtat gatattacag gtgcagaggt
     5701 gttgaaatat atcgatttta acgcaggtaa tattgctaaa caattattga atcagtttga
     5761 aggatttagc cctttaatta cgaatgaaat cgttagtcgt cgtcaattta tgacttcatc
     5821 aacattacca gaagcatttg acgaagtaat ggcagaaacc aagttaccac ctactcctat
     5881 ttttcataaa aatcatgaaa caggtaaaga ggatttctat tttataaagt taaatcaatt
     5941 taatgatgat acagttacat acgattcatt aaatgatttg cttgatcgtt tttatgatgc
     6001 gcgtggcgaa cgtgaacgcg ttaaacaacg tgcgaatgat ttagttcgat ttgttcaaca
     6061 gcagttgcac aaatatcaaa ataaattagc gaagttgatt gaagaatatg agcagtctaa
     6121 aaataaagat actgaacagt tatatggtga attgatcact gctaatatat atcgaattaa
     6181 gcaaggcgat aaagaagtga cggcattgaa ttattatacg aatgaagaag ttgtcattcc
     6241 tttaaatcct acaaaatccc catcagcaaa tgctcaatat tattataaac aatataatcg
     6301 tatgaaaacg agagaacgtg aattacaaca tcaaattcaa ttgacgaaag acaatataga
     6361 ttatttttca acaatcgaac aacaattaca tcatatttct gtccatgaca ttgatgaaat
     6421 tagagatgaa ttagcagaac aaggctttat gaaacagcgt aaaaatcaaa ctaagaaaaa
     6481 gaaagcgcag attcaattac aacattatgt atcaactgat ggcgacgata tatatgttgg
     6541 taagaataac aagcaaaatg attatttaac aaataaaaaa gctaaaaaaa ctcacacatg
     6601 gttacacaca aaagatattc ctggttcaca tgtcgttata tttaatgatg caccaagtga
     6661 tacgacaatc aaggaagcgg ctatgttagc aggatacttt tcaaaagctg gtaattctgg
     6721 acaaatacct gttgattata cattaattaa aaatgtgcat aaaccatcag gtgcaaagcc
     6781 tgggtttgta acatatgaca atcaaaaaac tttgtatgct acacctgatt atgaacttat
     6841 tcaaaaaatg aaacaatcat aatcaagttg tcgacaaata aacacctcac aaaagaaagt
     6901 agtcctttct tatggtgagg tgtttattta ttatttaatg cgttcattga aacgaagtat
     6961 cgtgttttta aataacgtat catcatttaa gttacaaatt atctttgtga ttttaaaaat
     7021 gagtgaaact aaaatgctaa tgtgtactta aaatagaaat acataggtaa aacgcatgca
     7081 taagaaaaag tgtttcttta tcattcaacg ctgtgcgaat ccttttactc aggtaatgct
     7141 aattgaaaac ttactccaaa cttatcttga acccaagcaa actctctgta tggtggcata
     7201 ttcgtttttg gcattaaaat ggcaccttca tcttttaatc cattaaatag tcgttccatt
     7261 tcaatagtat cttttactgt aacaaataat gaaatcgcag gattcattgg taattctgtg
     7321 ccactattag catcaatcgc catgaatact tgtccattta atgtaaatat tgagtgttgt
     7381 acagtcccgg gatcaccagg tccattttca ccatacttag ccattgttat aatctcacta
     7441 tcttcaaata agcttgtgta tagtttaaca gcttcttcag cttggttatt aaacattaaa
     7501 aatgtcgtga tttttggaat atccatgcta tgcctcctat agattgaact tctataacta
     7561 ctattatctt aacataatta aaatgacaaa agcgcgtcgt accgttatgt gcacgaatga
     7621 caaacgtctt attggcctgt tgattaaatc acatagttaa cgataaaaat tacgcaagat
     7681 taaaaatatg atacatatgt taataaatga tacttatcat aggtattaat gttttgactt
     7741 attgtcaaat ttgattacaa ttaaaattaa gttaaaggag ggacgttatg ttacattatg
     7801 tgattccagt attagtccta ttactcgtat tcttaattgg ttggatttta ataaagttaa
     7861 ttatccgcta ataaattttt tatataataa aagctatagt gtttgtaata ttcggatgcg
     7921 gttaagcagc ccctgatatt aaacactata gcttttttgt ttttaaattt gtccttgttt
     7981 acgtaatata tcctttacat gtttaaataa atcatcatta tgatctatag ttgttttatt
     8041 ttttatatgt ttttgcttcg ttgaactttc gtcgacatcg tgactactag atagattgga
     8101 atcatttgaa tgattgataa attcgttaca atgttttatc ttcccatata aatcattaga
     8161 tgttttgtca ttcatttgtt gaattcctcc ttctagtgtc tatattatgc taagttgtct
     8221 ctccattcaa ctaatgtttg gatatcttca ctagaaattt taccttcatt ttcagctact
     8281 tcaattaatt cattgtaatc acttaaagtg taaaaaggta gttgaatatt gctaaatgta
     8341 tcatctgctt ttttcaaacc gtaagtaaag atagctacaa cacctaatac ttctgcacct
     8401 gctagtttta aggcttcaac tgctgtgact gaagatcccc ctgtcgaaat taaatcttct
     8461 atcacaacta cttttttacc ttcactttta gcaccttcga tttgattttg cttaccatga
     8521 ctcttatttg atgaacgaac ataattcatt ggtaatttta atttttcagc aataaaagct
     8581 gcatgtggaa taccagctgt tgcagtacca gaaataactt ctacttcagg aaagtgttct
     8641 ttaattaagt taattaaacc atcgcggatt gcgcctcgaa ctaaaggata acctaacgta
     8701 acacggttat cacagtaaat cggtgattta atacctgaac tccatgtata taaatcattt
     8761 ggtgataatg ttacagcttc aatatctaat aatgattttg caatttcttt agccattata
     8821 ctaaccaact ttctttaatt ttatgataac tttcgactgg attgtcactt tgtgtaatcg
     8881 gtctaccgac tacaatatgc gtcgaaccaa gctgtcttgc ttcttccggt gtcgtaatac
     8941 ggtgttggtc attttgagat gcacctttag gtctaatacc tggtgttact tttaaaaatg
     9001 atgtacctaa cttttcagtc aacatacgac tttcaagagg tgaacaaaca acgccatcta
     9061 aaccagctgc atttgctaac ttggcataat ttaaaacggc ctcttcaatc gatgtttgta
     9121 tattttgttc gtgtcgtaac atgtcttctg ttgttgacgt aagctgtgtt actgcaataa
     9181 tttttgtatc ttgattatgt tttcttaatc ctttaatggc ctcagacatc atttttacgc
     9241 caccagcagc atgaacattt accagatcaa cattcaattt agctagtcct tccatcgcct
     9301 taccaactgt attaggaata tcatgcagtt ttaaatctaa aaatacatca tggcctcttt
     9361 cttttatctc attaattaat tgaggacctt cttgataaaa aagttccata cctactttta
     9421 cgaataatga ttcatcaaat aaatctaaaa attgatttac tttttctttt gattcaaaat
     9481 ctaatgcaat aattggtaaa tctttcatca tatatgtctc cctttatatt aagaaattca
     9541 ttaaaataat gaggctgtaa tataaacttc tttaagtaaa tggttaatta cagccccacc
     9601 gatacagttt gattgattta catttgacgc attgtaaatg tcatactttc aattacattc
     9661 gttaaggcat tagctgtatc taaagatgtc aaacatggaa taccattttc aactgtagta
     9721 cgtctaattt ggaagccatc cctttctact tctttacctt tagtcattgt atttataacg
     9781 atttgaacat caccattttg aatacgtgtt aataaatcat tttcgccacc aattttgcct
     9841 actacttctg caggtatgtc atactcagct aatttattag ctgttccaga cgttgctaaa
     9901 attttatagc caacttcatt taagcgttgt gccaatttaa caacttcctc tttatctttg
     9961 tcactgacgg tcattaatac tgtaccgtga tctttaactt caacgccact acctgttaac
    10021 cctttgaata acgccttttc taatgtagta tctttcccca tcacttcacc tgttgacttc
    10081 atttcaggtc ctaaagtaat atcaacattt ttcaatttat taaaactaaa tactggtgct
    10141 ttcacaaaga caccctcagc atatggttga accccttctt gataacccat atctgttagt
    10201 ttttccccaa tgattgctcg catagctaat tgtgccattg gaatatcagt aattttactt
    10261 aagaatggta ccgttctact agaacgtgga tttacttcta aaacatacac accatcgtga
    10321 gctataacga attgaatgtt gattaagcca atgatgttta aacctttagc taattttata
    10381 gtatagtcct caagtgttgc taactcgtct tctgtcaaag tttgtggtgg atatacagcg
    10441 attgagtcac cactatgcac accagcacgt tcaatatgtt ccatgattcc tggaataatg
    10501 accgtttctc catcacaaat cgcatcaact tcaatttctt taccagttaa atatctatcg
    10561 actagtaccg gatgttccgg actcgctttt acagcctggg tcatatagtt ttctaactct
    10621 ttgtcattgt ctacaatttc cattgcgcga ccacctaata cataagaagg tcttactaca
    10681 accggatatc cgatttctgc agcattcgct aatgcttcct caggtgatgt agctgttttc
    10741 ccttgtggct gtggcacgtt aatttttctt aatagtgctt caaattcttt tctatcttca
    10801 gcacgattta gattttctag tgaagtacca agtattttaa caccatgttt agccaatttg
    10861 tctgctaaat taatcgctgt ttgtcctcca aattgtacaa cgacaccttt aggtttttct
    10921 aaattaatga tattcatcac atcttcttca gttaaaggtt caaagtataa tttgtcagaa
    10981 attgagaagt ctgttgaaac tgtttctgga ttgttattca caattatcgc ttcgtaccct
    11041 gctttttgaa ttgcccaaac ggcgtgaact gtcgcatagt caaattctac accttggccg
    11101 attcgaattg gtccagagcc taatactaag attttttctt tgtcagtaac tatggattca
    11161 ttttcagttt cgtatgtacc ataataatat ggtgttgaag attcaaattc agctgcgcaa
    11221 gtatcaacca tcttgtaaac aggtttaata tcattttcca tacgcaattg atatacttct
    11281 tcttccgtca tattaaagcg atgcgctatt gttttatcac taaatccata atcttttgca
    11341 tatttaagat attctaaatc accttgatgc tcttttaatt gatgctcaat atcaataatg
    11401 ttttggaact tgtgtaagaa gaagtaatca atctgagtca tattatgaat ttcttctaat
    11461 gttgtgcctc ttctaattgc ttcgccgatg aaaaataatc gttcatcatc ttggtgtgaa
    11521 atacgttctt taatataatc aagatcgaag ctttcaccat ttggtaatcc taagtgatgc
    11581 acaccatact caagtgatcg aattgctttt aacaatgatt cttcgtaagt tcgaccaatg
    11641 gccataactt cacctgttgc tttcatttgt gtgccaagct ctcgttctcc tttttcaaat
    11701 ttatcaaaag gaaatcttgg tatttttgaa atcacatagt ctaaagttgg ttcaaacgct
    11761 gcataagatg ttcctgtaat tggatttaac atttcatcta atgttagacc aaccgcgatt
    11821 ttagcagcta attttgcaat aggatatcct gttgcttttg aagctaacgc tgatgaacgt
    11881 gatacacgcg gatttacttc tataatataa taatcgaatg aatggggatc taatgctaat
    11941 tgaacattac aaccaccttc gatacctaaa gctcgaataa cttttaatga aacatcacgt
    12001 aacatttgat actcaacatc tgataatgtt tgactaggag ccacaacaat tgaatcgcct
    12061 gtatgaatac caactggatc aatattttcc atgttacata caacgatggc attatcgttt
    12121 ttatcacgca ttacttcgta ttcgatttct ttaaaacctg cgatagattt ttctaataaa
    12181 cattgcgttg ctggactata atgaagacca tttgagacga tttcatgtaa ttcttcatca
    12241 ttatgacaaa taccgcctcc ggtaccaccc atcgtaaatg ccggtctaac aattagcggg
    12301 tatcccactt gctctttgaa tttaaaggct tgctctactg tatttacaat gtcactctct
    12361 ggtacaggaa cgtttaaatc attcattaat gttctaaaca tttcacggtc ttctgcttgt
    12421 tgaattgatg ttagctcagt tcctaataat tggacgttat tatcttgaag cacaccactt
    12481 tcgtgtagtt gaatcgccat gtttaaacct gtttgaccac ctaaagttgg gagtaaagcg
    12541 tcaggttgct ctttacgtat aattcgcgct ataaaatcat gagttaacgg ttcgatatat
    12601 actttatccg caatttcctt atcagtcatg attgtcgctg gatttgaatt tacaagaata
    12661 actcggtatc cctcttcttt taaagctaga catgcttgtg ttccagcata atcaaattca
    12721 gctgcttgac ctatgataat tggcccagac cctattacta aaattgtttt gatatcatta
    12781 cgtttaggca ttgatatgac gctccttttc cttaaaatta gtcatcattg ctacaaaatc
    12841 atcaaataga tagtttgaat ctgacggtcc tggatttgct tcaggatggt attgaacaga
    12901 aaatgctggt aatgttttat gttttaaacc ttctacagta ccatcattta atgcaagatg
    12961 agtaacttct aaatcagtac tttttaacga atctatatct attgcatatc catggttttg
    13021 actcgtaata tcaacttttc cagtctctaa atttttaact ggatggttcg caccacgatg
    13081 accaaacttc attttaaatg agcttgctcc ttgagataat gcaaaaagtt gatgtcctag
    13141 acagatacca aagaacggaa ttttccctaa aattccttga atcattggaa tcgcacattc
    13201 tacaacttca ggattacctg gtccgtttga tagcataacg ccatctggag ccattgctaa
    13261 aatttcttcg gcagtagttg tatatgggac aactgtgacg ttacaacctc tgacgtttaa
    13321 ttctcgaaca atattttgct tcttaccaaa gtctacaagt acgacactta gatccttacc
    13381 tgtcgaaaca tacggtgttt tagtcgaaac gatttctact tcgtttttag gtaattctac
    13441 ttgttgtaaa tgtttgacaa gttgatcaat atcttctttt cgatcagtaa aaccagcttt
    13501 taacacaccg tgttgtcgaa ttttacgcgt aatacttctt gtatcaacac ctgcaatccc
    13561 tggaatttga tgcaattcta aaacgtcatg aagtgtcttt tgctgtctaa aattacttgg
    13621 atgcgcactc gcttctttca ctacaatacc gtttaatgta ggtactaatg attcaaaatc
    13681 gtctctattg ataccataat taccgattaa tggatacgta aaagtaatga tctgacctgt
    13741 atatgatgga tctgaaatag tttcttgata acctgtcatc gctgtattaa atacaatttc
    13801 tcctacagtt aagttatcag atcctaaacg gtagccttcg taaaaagaac cgtcttctaa
    13861 cactagataa cgtttgcttt gcatattatt tatccccctc aaatttaact tcgccttcaa
    13921 ccattgttaa gatcggattt ccataaactt tatagccgat aaatggtgta ttatctgctt
    13981 ttgataagaa atcttctcct ttaatttctt gttcactatc taaatcaatg attgttaaat
    14041 ctgcataacc attttctttt aatgtgccgt attctaaatt aaatgtctca catggtttaa
    14101 ttgttaagta atctactaat tgttgtaatg tccaatcacc attttttaca aaatgcgtat
    14161 ataataatgg gaatgctgtt tcactaccaa caattccgaa tggtgctttt tccattggtt
    14221 gtgctttttc atcacgtgca tgtggtgcat ggtctgttgc gatacagtca attgtaccgt
    14281 ctagtaaccc ttctaacaaa gcctctctat cttcagtact tctcaatggt ggattcattt
    14341 tataaatggc attattacca ggaatatcat cttctgttaa caataaatgg tgtggtgtaa
    14401 cttcagctgt aacatgaatg cctgcgcgtt tagcgtcacg aatgactcta acactttctt
    14461 tagtagaaac atgacataca tgataatgac aacctgctgc ttcagctaat agtacatctc
    14521 ttgcgatttg aacagattca caaatgtttg gaatacctgg tatacctaac tctttactgc
    14581 gtttcccttc atgcattgca ccaccataga ttaatgaatt atcttcacag tgtgctacga
    14641 tggctttgtt tacttttgca gcttcaatca tgccttcata catcatgctt gcagtttgta
    14701 ctcctacacc gtcatctgta aacgcaaagg caccttcttt tactagtgct gggaaatcaa
    14761 ccaattcttt acctaattga cgtgttgtaa ttgaagcata aggtaataca cgtacttgag
    14821 cattgtcatc gattaatttt tgtaaagctt caaaatgttc tacagaatct ggtaccggtc
    14881 ttgtgttagg cattggacat acagttgtaa atccgcctct agcagcagct ttagtaccag
    14941 tttcaattgt ctctttatat tcaccaccag gttcacgtaa atgaacatga acatcgacaa
    15001 atccaggtga cacaaagtga cctttcgcat ctatgatgtc aacaccattg cttggttcaa
    15061 ttgcaggtgc aatttgttta attaccttac catcaattaa aatatctgct tgttgtaatt
    15121 cgccattttg taatacttta ccgtttttaa ttaatttcat cattttcgtc cccttaccta
    15181 ttttaataat tcatcaatga ctgccattct taagtaaacg ccattttcca tttgcttaaa
    15241 aattcttgat tttgaagctt ctactaaatc gctttgtatt tctactcctc tattcacagg
    15301 tgccggatgc ataacaatag catgttcttg taatttgtta tagcgtactt catttaagcc
    15361 atgcttttga tgataatcat ctgctgcaaa tctagtttct tctgcaagcc catgcctttc
    15421 atgttgaatt cttaataaca taactatatc tactgtttct ataacatcat ctatatttac
    15481 ataaggtgct tctaaagaat catcaatcca agcatttggg ctattaaaca ttacgtttgc
    15541 acctaatgct tttaaactat ggtaattact acgtgcgaca cgtgaatttt taatgtctcc
    15601 acaaatcaat acattcaagc cttcaaaata tccatattct tcatatatcg tcattaaatc
    15661 aagtaaactt tgtgttggat gttgtccact accatcacca gcattcgcaa ttgggatgtt
    15721 aatattcgct aatttttcat agtagttatt aaacggatgt ctaatgacta ataaatcaca
    15781 gccaatactt tctaaagttt tacatgtgtc atataaagat tcaccttttg aaacagatga
    15841 tgttgatgtt tcaaagctaa tcgtttttaa ccctagctta agttctgcca tttcaaaact
    15901 acattttgtt cgagtagaat tttcaaagaa taaatttgcg acatatttcc cttcaaagtt
    15961 tggtagttga cgttcaccag atttaaattg acttgccttt tggataagtt tgtatatttg
    16021 atctgtagat aaatgttcca ttgataataa atgattcata aaagcgccct ccttaatttg
    16081 taaatcttaa ttgttttgtt ttttctcttt aggtaagatt aagttcaaaa taattcctga
    16141 aagtgcagct aatgccatcc cctcaatttg aaggttgata ccaatttctt ttaagttaaa
    16201 tactaaatta ccgataccta caactaaaat tacagaagct ataactaaat ttcgattgtt
    16261 cgcaaaatct actttgcttt caactaacat tcttaagcca cttgctgcaa taataccgaa
    16321 aagtaatata gatactcctc ccataactgg tgtaggtata gaagaaatta atgcagtgaa
    16381 cttaccaatg aatgctaaaa caattgctat aactgctgca ccaccaatga cgtaaatact
    16441 gtatattctg gtaatcgcta atacaccgat attttcaccg tatgttgtac ttggtggtcc
    16501 accaataata ctggcaaaca ttgtagaaac accatcacca atgattgatt tatcaagtcc
    16561 tggcttttca aagaagtttc tacctacgat tttattcaat accatttggt gcccaatatg
    16621 ttcacttact gttacgaaca caatcggaat cattacaagt acaagtccta agtgaaatga
    16681 aggtacataa tctttaaatg gcagataaat atgagggaaa tctatccatt ttgctgacat
    16741 aattgcatca aatttcacaa tccccataaa aatcgctacc acataaccta cgataatacc
    16801 tacaagaact ggaattaaag ataggaatcc tttaaaaaat ccttgaacga ctattgttac
    16861 aagcaaggtt atcattgcaa caattaagaa actgatattg taacctttca tatctccagg
    16921 attttcatac attgccatat tgactgcagt aggcgctaag cttaaaccaa ttaccatgat
    16981 gactggtcca acaacaactg gtggtaataa tttcattaac catgctgtcc cacttaattt
    17041 gattagaatc ccgatgatga cgtacataac accactcatg aataatgcta caagcatgtc
    17101 tcctaagcta tgcgtactta atcccgtgat aattggcgtg ataaatgcaa agctagatcc
    17161 caagtatgct ggtatttgcg ccttcgttat taagatataa agtaatgtac cgattcccga
    17221 agctagtaac gctgctgata ttggtagtcc tgttaagaat ggtactagta ctgttgcgcc
    17281 aaacatcgca aataaatgtt gtaagcttaa aaatgcccat tgcgctggtt gtggtttttc
    17341 atttacatct agtacgggtt ttactgttcg ttcaaacatt tcatcatttt gcataatatt
    17401 catttcctcc gataataaaa aaatctcttt acatcagtat atgtaaagag acaaaaagtg
    17461 tgacaagttg ctacaagtca ttttcgtcca tagaaattga cttatagttg tcgaacatga
    17521 gggtattatt agataaacaa gcatatgaaa acttatttat cattcaactc ccccaccttt
    17581 ttcagtctct cgtactgaat taaaaggggt attatttaat tataactgca tttctttgat
    17641 ccatttcttc taaatagaca cttaccgttt cctctttaga agtaggtata tttttaccaa
    17701 caaaatctgc tcgaattggt aactcacgat gtcctcgatc aaccaaagca gctaaaccaa
    17761 ttttaatagg tctagcattt agcaaaatag catcaagtga agcacgaacc gttcgaccag
    17821 tatacagcac atcgtcaata atgatgacta ctttatctgt aatatctgtg tcgatgtcta
    17881 ttgcgtcttt tgtcgtaagt gatgacatgt gctctatatc atctctaaag tatgtaatat
    17941 caattgttcc agtaggtata cgttgttgct caatttgatg aattttatct tgtatacgat
    18001 tcgctaaata ttcacctctt gttttgatac ctaaaagaat taaattatca gtacctttgt
    18061 tatactccaa aatttcgtga gcgattctcg tcactgtacg ttgtattgcg gcatcatcca
    18121 ttatgatacg ttcagacata ttctcaccct ttcaaaattt agaaacaaaa aacctcatgt
    18181 ctttaatatc gacatgaggt ttagtatata caatcagcct atttacatag aagaatcgta
    18241 gtattactac ttattcttgc atatgtgaaa gtgttatgat cattttagta tatatctata
    18301 ttttcatgtc tttgacgtct ctctggacgc tcaattaaag actctttatg ttgtacttaa
    18361 actgttataa ggataatact aatcaggcgc tttgtcaatc tatattgaaa aattaaccat
    18421 actcagtaaa aatgttaacc ttaatctatt ttataatgtg ttttcattta aaaaaaacga
    18481 aaacgataaa cacttatagt ttacataata atgttatcgt aattatagca cgacacaatt
    18541 atgcatctct ttttcgaatt gtatctaata aatcttcaaa gtcttgtggt aattcagcat
    18601 gtctttcaat atattcacct gttactggat gttcgaatcc aataagtcca gcatgtagag
    18661 cttgaccacc aatatccaat gtctttttcg gtccatactt tggatcacca actaatggga
    18721 agccaatata tttcatgtga acacggattt gatgcgtacg tcctgtttca agttgacatt
    18781 caacaagcgt ataatcttta aaatgttcta gtacgttaaa atgtgtcact gcttccttac
    18841 catcatcaac aacagccata gattgacgat catttttgtt tctaccaatt ggcgcatcga
    18901 ttgtaccgta atcatgagga atattcccgt gaactaaagc gatatatttt cttttaacag
    18961 atttatccat taattgttct acaagcccac gatgagcaat atcattttta gcaaccatta
    19021 ataaaccaga agtatccata tctatacggt gaacaatacc tggacgaatt tctccattaa
    19081 tacctgacaa atttttaatt tgatacatta aaccattaac taatgtattg gtataatgcc
    19141 ctggtgatgg atgaactacc atgcctttcg gtttatatac aactgcaaca tcgtcatctt
    19201 cataataaat atctaaattt aaattttcag gtagaatatc agcttcaacc acttcttttt
    19261 cagtgacaac tatatgatca ttaagtttca ctttataatt agatttaaca actttatcgt
    19321 ttgcaacgac taaacctgct ttaatccaat cttgtatctg gttacgagac caatcattat
    19381 ttaattcagg cagcaactta tctacacgca tacctgtttg ttctttatct gtaatgttaa
    19441 attcataagt ctccattact taacctcctt ctccttttta ttggaagtat cctttaataa
    19501 ggcaataata attaatatta c
//
LOCUS       DAHHYJ010000037        19012 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000037 DAHHYJ010000000
VERSION     DAHHYJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19012
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.037"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            116..1306
                     /locus_tag="NBK22_000992"
     CDS             116..1306
                     /locus_tag="NBK22_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCD2414190.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1415..2659
                     /locus_tag="NBK22_000993"
     CDS             1415..2659
                     /locus_tag="NBK22_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCD2414191.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNTHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(3181..4110)
                     /locus_tag="NBK22_000994"
     CDS             complement(3181..4110)
                     /locus_tag="NBK22_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD2414192.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4352..5731)
                     /gene="argH"
                     /locus_tag="NBK22_000995"
     CDS             complement(4352..5731)
                     /gene="argH"
                     /locus_tag="NBK22_000995"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCD2414193.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHHIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKERLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5721..6926)
                     /locus_tag="NBK22_000996"
     CDS             complement(5721..6926)
                     /locus_tag="NBK22_000996"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCD2414194.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7277..8608
                     /locus_tag="NBK22_000997"
     CDS             7277..8608
                     /locus_tag="NBK22_000997"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCD2414195.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEDNIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8933..9508
                     /locus_tag="NBK22_000998"
     CDS             8933..9508
                     /locus_tag="NBK22_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD2414196.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGSILGIVISWLGLISGTFTVYLICKRLVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTH
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9513..10037
                     /gene="lepB"
                     /locus_tag="NBK22_000999"
     CDS             9513..10037
                     /gene="lepB"
                     /locus_tag="NBK22_000999"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD2414197.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPSLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDHDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            10053..10628
                     /gene="lepB"
                     /locus_tag="NBK22_001000"
     CDS             10053..10628
                     /gene="lepB"
                     /locus_tag="NBK22_001000"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD2414198.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYTIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10788..14264
                     /gene="addB"
                     /locus_tag="NBK22_001001"
     CDS             10788..14264
                     /gene="addB"
                     /locus_tag="NBK22_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCD2414199.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKADPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFKQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFEQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLYDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEEILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWSDIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14265..17918
                     /gene="addA"
                     /locus_tag="NBK22_001002"
     CDS             14265..17918
                     /gene="addA"
                     /locus_tag="NBK22_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCD2414200.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKAYDLFSMMDSVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTIDGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVKYDEAAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYRYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            18084..18986
                     /locus_tag="NBK22_001003"
     CDS             18084..18986
                     /locus_tag="NBK22_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCD2414201.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
BASE COUNT         6601 a         2822 c         3482 g         6107 t
ORIGIN      
        1 acttagtgat tatttcatct tttttggtgg aaaagtatct gctttgaaaa ctaatgtaat
       61 tatttggata atgtttcatg tataatagtt ataaatgatg aaaaggggag atatgatgac
      121 taaatctgaa aaaattattg agttaacaaa tcattacgga gcacataatt atttaccatt
      181 gccaattgtc atttcagaag ctgaaggggt atgggttaaa gatcctgaag gcaataaata
      241 tatggatatg ttatctgcat attccgctgt taaccaaggt catagacatc cgaaaattat
      301 tcaagcatta aaagatcaag ctgataaagt gactctagtt tcacgtgctt ttcatagtga
      361 taacttaggt gaatggtacg aaaaaatttg taaactggca ggtaaagata aagctttacc
      421 aatgaataca ggtgctgaag cagtagaaac agctttgaaa gcagcacgac gctgggcata
      481 cgatgttaaa ggaattgagc caaataaagc agaaatcatt gcatttaatg gtaacttcca
      541 tggtcgaaca atggcgccag tttcattatc ttcagaagca gaataccaac gtggttatgg
      601 tccgttatta gatggattta gaaaagttga ttttggagat gtagatgcat tgaaagctgc
      661 aattaatgaa aatactgcag cagttttagt agaaccaatt caaggtgaag cgggtataaa
      721 tataccgcca gaaggatatt tgaaagcaat tagagaatta tgtgatgaac ataatgtctt
      781 atttattgct gacgaaatcc aagcaggatt aggtcgttcg ggtaaattat ttgctacaga
      841 ttgggataat gtaaaacctg atgtctatat tttaggtaaa gcactaggtg gtggtgtctt
      901 cccaatttct gttgtattag cagataaaga agtattagat gtctttacac ctggctcaca
      961 tggttcaaca tttggtggta atccacttgc ttgtgctgca tcaattgctg cattagatgt
     1021 tatcgttgat gaggatttac caggtcgctc tttagaatta ggagattatt ttaaagaaca
     1081 attaaagcaa attgatcatc catcaattaa agaagtccgt ggacgtggtt tatttatagg
     1141 tgtggaactt aatgaaagtg ctagaccata ttgtgaagct ttgaaagaag aaggcttatt
     1201 atgtaaagaa acgcatgata ctgtcattcg ttttgcacca ccattaatta ttactaaaga
     1261 agaattggac cttgcacttg aaaaaataag acatgtattt caataatgtc aaaaagttgt
     1321 aacaatcagt ttgtaagggc tttaaaaagg aatacgttat gttacaatat gtttgaaagc
     1381 gaaatcatta ggtaaagaag aggcgaaaag gatcatgact gagaacaata atttagtaac
     1441 ttctactcaa ggaattatta aagaagcatt gcataaattg ggatttgacg aaggaatgta
     1501 cgatttaatt aaagaacctt taagaatgtt acaagtgcgt atccctgtac gaatggatga
     1561 tggcactgtt aaaacattca caggttaccg tgcgcaacat aatgatgctg ttggaccaac
     1621 aaaagggggc gtgcgtttcc acccagatgt tgatgaagaa gaagtaaaag cattatcaat
     1681 gtggatgact ttgaaatgtg gcattgtaaa cttaccatac ggtggtggta agggtggtat
     1741 cgtttgtgat ccacgtcaaa tgagcattca tgaagttgaa cgtttatcac gcggatatgt
     1801 aagagcaatt tcacaattcg taggtccgaa caaagatatt ccagcaccag atgtatttac
     1861 aaactcacaa attatggctt ggatgatgga tgaatatagt gcattggata aatttaattc
     1921 accaggtttc atcacaggta aaccaattgt attgggtggt tctcatggac gcgacagatc
     1981 tactgcacta ggtgtagtta ttgcaattga acaagctgca aaacgtcgta atatgcaaat
     2041 tgaaggtgcc aaggttgtta ttcaaggttt cggtaatgcc ggaagtttct tagctaaatt
     2101 cttatatgat ttaggtgcaa aaattgtagg tatctctgat gcttacggtg cattacacga
     2161 tccaaatggc ttagatatag attatttatt agaccgtcgt gatagttttg gtacggtaac
     2221 aaatttattt gaagaaacaa tctcaaataa agaattgttt gaattagatt gtgacatttt
     2281 agtaccagcg gctatttcaa accaaattac agaagacaat acacatgata ttaaagctag
     2341 tatcgttgtt gaagctgcta atggacctac aacaccagaa gcaacacgta ttttaactga
     2401 acgtggtata ttattagttc cagacgtatt agcaagtgct ggtggtgtaa cggtttctta
     2461 cttcgaatgg gtacaaaata atcaaggtta ttattggtct gaagaagaag ttaatgaaaa
     2521 gctacgtgaa aaattagaag cggcatttga tacgatttac gaattgtctc aaaaccgaaa
     2581 aatagatatg agacttgcag catatatcat aggtattaaa cgtacagcag aagcagctag
     2641 atatcgtggt tgggcataat taattatcat atgtgattta acgagcttgg gacagaaaac
     2701 aaagccctaa gctcgttaat tttattttag cagtagttga ctgtaaaaca atgcccgtgt
     2761 aacacgctct tttcaaaaat agtcggggcc ccaacacaga ggctggtgga aagtcagctt
     2821 acaatagtgt gcaagttggc tggcccccaa cactgagaat ttttaattaa accaggctgg
     2881 gacataaatc aatgttctat gctctacgaa gttattttag cagtagttga ctgtaaaaca
     2941 atgcccgtgt aacacgctct ttccaaaaaa agtcggggcc ccaacacaga gaatttcgaa
     3001 aagaaattct acaggcaatg caagttgggg tgggacgacg aaataaattt tgcgaaaata
     3061 tcatttctgt cccactccca aaaagacagc agtaggataa tttcaatttg gaaatatctt
     3121 actgctgttt tttatagtaa tagctatatt tttgtattta tgttttctag tttaacatta
     3181 ctacttaatg acttctttat atttatcagc gaaatttgta aagacaccat caacgccata
     3241 tttatttaat cgtaacatat cagctttttc attcactgta taaggatgta ctataaatcc
     3301 taagtctttt aaatgatggg tattttgttc agttaaatct gtataatcag gacctaatcc
     3361 aatcgcataa gagcgtatct cttttaagcg ttggtcgtta aattgttgta gttcaccttt
     3421 atcaactaat tttactaatg gcacatgctt attttgacga tgaattttct ttaaactttc
     3481 gtcagaaaat gattgaatca ttacatgtcc attttttaat ttattgttat ttaaaaggtg
     3541 atgctttttc aatgaagcta ataattgttc ttccattcct gggtatacat caggtgactt
     3601 tgtttcaata taatagtttg cattcgggcc ataacgttct aaaatttcat ctaaagtggg
     3661 tactttagca tttttataac ttgctcttgc gtattttgga tattttttat taaaccaact
     3721 tcctgcatct aactgtttta attcatcaag ggtataatcc tcaactttac cgtgtccatt
     3781 tgttgtacgg ttaacagttt catcatgcat agcaactaaa tggccatctt tggtacgttg
     3841 taaatcaatt tcgatataag atgcttttaa ctcattatga ctcttatcat atgcttgaaa
     3901 cgtatgctcg ggtgcatagc cacttgcgcc acgatgtgcg atagtagtga atcgctcatt
     3961 tgttaaattt gtatgccatt gaatagcctg aggtttattt gcaatttgat ttgtttgttc
     4021 agcgccagca gtaggtactg ataaaaatcc catagtaaaa acagcagaag cagccataaa
     4081 tttagtgaag cttttcgaag agttagtcat agtctttatc ctttcatctc tttatttgta
     4141 gtctaacaat tttaaaatac tacaaaaacc tgcttgtgaa aacgctttac acatttcttt
     4201 tacaatttat aacatttttg taatataaat ttaaaagaat tatcaaacat tttaggaatc
     4261 ttaaaaatgt gatgttcaac aagacgaaga gagcattaaa atacccacaa tgtaatttta
     4321 atagacattg tgggtaggta gattaacgta tttattgtga tagtaattgt ttagcaacat
     4381 caagttgttg tttgaccgat gattgacctg ttgaaccgta actttgacgt cgttttaaac
     4441 aattttcagg ctgcaaataa tcgtaaatat cggcatcaat actagaatga tgttgttgat
     4501 atgttgctaa aggaacatct aataaataat gaccttgttg tatacattct aagacgattt
     4561 ttcctacaat ttcatgtgca gttctaaatg gaatattttt agttactaaa taatctgcta
     4621 gttccgttgc atttgaaaaa tcttctttaa cagtttgatt gagtcgttct ttattaattg
     4681 tcatcgtttg aatcatacct tcgaaaatac gtaaagaacc tttaattgta tggacagcat
     4741 cgaataaacc ttctttatct tcctgcatat ctttgttata tgctagaggt aatcctttta
     4801 aagtcataag catgctcatt aaatgaccag tcgttcgacc aactttacct ctaattaatt
     4861 ctgccatatc aggatttttc ttttgtggca taatagatga gccagttgaa aatgcatctg
     4921 ataatgtaat gaatttagct tcgtctgtgg accagaaaat aatttcctct gcaaagcgtg
     4981 ataagtgaac catcgttaaa gaaatattat gcaatgtttc aataatatag tctctgtcac
     5041 taacagcatc taggctattc tcatagagac tgccaaagtt caacaatgct gttgtctcgt
     5101 gtctatcgat agggtatgtg gtaccactta aggctgctgc acctaaagga ttaatatcga
     5161 ttcgttttaa actatcttca aatcgttgtt ggtctcgttg taacatccaa aaataagtca
     5221 taatatgatg tgcaaatgaa attggctgtg cacgctgtaa atgagtataa ccaggcataa
     5281 ttgtatcaac attattggaa gcgatgtcta caattacact ttgtaacgac ttaattaatg
     5341 cgatgatatc ttgcacttgt ttcttagtgt acaagtgcat gtctgttgca acttgatcgt
     5401 ttctactgcg tccagtatgc aacttaccac cagcatcacc gatacgttta attaattcat
     5461 gttcaatatt taaatgaata tcttctaatg atgcactaaa ttgaatttga tcttgatgat
     5521 aatcatgttg aatagatttt agtccttgta taatttgttc gctgtcttgt tgactaataa
     5581 tgccttgatt cgcaagcata gttgcatgtg caatgctgcc ttcgatatct tgatctatga
     5641 gcgtttgatc aaaagtaatg gatgcgttaa agtcgtcaac ccactcttca ggttgtactt
     5701 caaatctacc gccccaagct ttattgctca ttgctatagc ctccatgcaa catcgcattt
     5761 acttgagtag gtaaaccata gatatcgata aagccaacag cagcgtcttg attaaatgca
     5821 tcttcttttg tataagttgc taatttttca tcatataatg tgtaaggtga ttttctacca
     5881 ttcacgatgg cattaccttt gaataattta attctgacat caccacttac gtattgctga
     5941 gtactatcaa taaataattt caagctatca gttaaaggtg agaaccaaag tccattgtat
     6001 agttgttcag caaattgctt ctcaatgatt ggtttaaagt gtgcgacatc tttcgttaac
     6061 gtaatcgttt ctagtgcttt atgcgctttt aaaataactt ctgcagcagg tgcctcataa
     6121 atttctcttg atttgatacc tacaagtcta ttttctacat ggtcaattct tccgatacca
     6181 tgcttaccag ctaatgcatt caacgttaaa attaaatcgt ctaattcata tgttttgcca
     6241 tcaatttgaa ctgggatgcc tttatcaaac gttaaaatga tttcatcagc agtatctggt
     6301 gtttcttcta aagcatttgt tagatcgaac gcatcctctg gtggcgcagc ataaggatct
     6361 tctaaaatac cacattcatt cgctctgccc catagatttt gatcgataga ataaggtgaa
     6421 tcatggttga ttgatacagg gatattatgt ttaattgcat aatcgatttc ttcttcacga
     6481 ctccatgccc actcacgtac aggtgcgaat gctttcaatg atgggtttaa tgctttaatg
     6541 gcaacttcga aacgtacttg gtcattccct ttaccagtac aaccatgtgc aatacctact
     6601 gaatttgttt tctcagcaat ctctactaat tttttagcga ttaatggtct tgataaagct
     6661 gaaactaatg gatatgcatt ttcatacatt aaatttcctt tgattgcata acttacatac
     6721 tcatcactaa attcttttgt tgcatcaata atatgacatt caactgctcc catatctaaa
     6781 gcttttttat aaacgatgtc taaatcttta ccttcaccaa catctaggca acaagctaca
     6841 acgtcgtatc ctttgtcgat aagccattga acggccacac ttgtatctag tcctcctgaa
     6901 tatgctaaaa caattttctc tttcataaaa ttcacctcat tgtagttatc tgaatatttc
     6961 gtatgaacac atgatagcaa acttttgatg aaaataaaac cattatttta catatttatt
     7021 cataaaatgc gatagagata gttagagttg taagtataaa tgcttattta tcaatgttta
     7081 aaagtatttt aaaaaacatg caaattgtat aaaaaatgtt tgagtgtttg acgccagttt
     7141 gaaatgtttg ttgaaaaata ggtgtgaaac gtcacataaa tatcaatttt attgattaag
     7201 tacaaacttg tgaattgaat cgcaaacgta aacttagtaa aattaagata ttaaatgaaa
     7261 caggaggctt ttatagatga ctcatattca attagatttt agtaaaacgt tagaattttt
     7321 cggtgaacac gaattaaaac aacaacaaga aattgttaaa tcaattcaca aaacaattca
     7381 tgaaggtact ggtgcaggta gtgacttctt aggctgggtt gatttaccag ttgattacga
     7441 caaagaagaa ttttcaagaa ttgttgaagc atcaaaacgc attaaagaaa attctgatgt
     7501 tttagtagtc atcggtattg gtggttctta cttaggtgca cgtgcagcaa tcgaaatgtt
     7561 aacgtcatca tttagaaaca gcaatgaata ccctgaaatt gtatttgttg gtaatcactt
     7621 atcatcaaca tatacgaaag agttagttga ttatttagca gacaaagatt tctctgtaaa
     7681 cgttatttct aaatctggta caactacaga accagcagtt gcatttagat tgttcaaaca
     7741 attagttgaa gaaagatacg gtaaagaaga agcacaaaaa cgtatatttg caacaacgga
     7801 taaagaaaaa ggtgctttaa aacagttggc tacaaacgaa ggttatgaaa cgtttatcgt
     7861 acctgatgat gtaggtggaa gatattctgt tttaacagca gtaggattat taccaattgc
     7921 aacagctgga attaacatcg aagctatgat gattggtgct gcaaaagcac gtgaagaatt
     7981 atcttcagat aaattagaag acaacattgc ataccaatat gcgacaattc gaaacatttt
     8041 atatgcaaaa ggttatacaa cagaaatgtt gattaactat gaaccatcta tgcaatactt
     8101 taatgaatgg tggaaacaat tatttggtga atcagaaggt aaagacttca aaggtatcta
     8161 tccttcaagt gccaactaca caactgattt acattcttta ggtcaatatg tacaagaagg
     8221 ccgtcgtttc ttattcgaaa cagtggtaaa agtaaatcat cctaaatatg atattactat
     8281 tgaaaaagat agtgatgatc tagacggatt aaattatttg gctggtaaaa caatcgacga
     8341 agttaacaca aaagcattcg aaggtacatt attagcgcat actgatggtg gtgttcctaa
     8401 catggtagtg aacattccac aattagatga agaaactttc ggttatgtcg tatacttctt
     8461 cgaacttgct tgtgcaatga gtggatacca attaggtgta aatccattta accaacctgg
     8521 tgtagaagca tataaacaaa acatgttcgc attattaggt aaacctggtt ttgaagactt
     8581 gaaaaaagaa ttagaagagc gtttataaaa tacattactt caatgattag tgaagtttga
     8641 aaagatagaa ctagacgtta actatttaaa gcatattttc gaggttgtca ttacaaatgt
     8701 aaaaatgtaa tgacaacctc gtttttattt atatgcaaga actaggttac tagctaatgt
     8761 gacaagatgt taagagaaaa ttaaagaaaa aataacatct gtcatacaat aatattgtta
     8821 tactactaga gactgattta ttagcatgat tacatgttaa tgtttcttta cttagtaatt
     8881 aactttataa tgtaagaata attatcttca gccaaagaaa gggattgatg atttgtcgtt
     8941 tcatcaagta gaagaatggt ttgagatatt tcgacagttt ggttatttac ctggatttat
     9001 attgttatat attagagcga taattccagt atttccttta gcactctata ttttaattaa
     9061 cattcaagct tatggatcta ttttaggtat agtgattagt tggcttggat taatttctgg
     9121 aacatttaca gtctatttga tctgtaaacg attggtgaac actgagagga tgcagcgaat
     9181 taaacaacgt actgctgttc aacgcttgat tagttttatt gatcgccaag gattaatccc
     9241 attgtttatt ttactttgtt ttccttttac gccaaataca ttaataaatt ttgtagcgag
     9301 tctatctcat attagaccta aatattattt cattgttttg gcatcatcaa agttagtttc
     9361 aacaattatt ttaggttatt taggaaagga aattactaca attttaacgc atcctttaag
     9421 agggatatta atgttagttg tgttggttgt attttggatt gttggaaaaa agttagaaca
     9481 gcattttatg gggtcgaaaa aggagtgaca tcgtgaaaaa agttgtaaaa tatttgattt
     9541 cattgatact tgctattatc attgtactgt tcgtacaaac ttttgtaata gttggtcatg
     9601 tcattccgaa taatgatatg tcaccaagcc ttaacaaagg ggatcgtgtt attgtaaata
     9661 aaattaaagt tacatttaat caattgaata atggtgatat cattacatat aggcgtggta
     9721 acgagatata tactagtcga attattgcca aacctggtca atcaatggcg tttcgtcagg
     9781 gacaattata ccgtgatgac cgaccggttg acgcatctta tgccaagaac agaaaaatta
     9841 aagattttag tttgcgcaat tttaaagaat tagatggaga tattataccg cctaacaatt
     9901 ttgttgtgct aaatgatcat gataacaatc agcatgattc aagacaattt ggtttaattg
     9961 ataaaaagga tattattggt aatataagtt tgagatatta tcctttttca aaatggacga
    10021 ttcagttcaa atcttaaaaa gaggtgtcaa aattgaaaaa agaattattg gaatggatta
    10081 tttcaattgc agtcgctttt gtcattttat ttatagtagg taaatttatt gttacaccat
    10141 atacgattaa aggtgaatca atggatccaa ctttgaaaga tggcgagcga gtagctgtaa
    10201 acattattgg atataaaaca ggtggtttgg aaaaaggtaa tgtagttgtc ttccatgcaa
    10261 acaaaaatga tgactatgtt aaacgtgtca tcggtgttcc tggcgataaa gtagaatata
    10321 aaaatgatac attatatgtc aatggtaaaa aacaagatga accatattta aactacaatt
    10381 taaaacataa acaaggtgat tacattactg ggactttcca agttaaagat ttaccgaatg
    10441 cgaatcctaa atcaaatgtc attccaaaag gtaaatattt agtgcttgga gataatcgtg
    10501 aagtaagtaa agatagccgt gcgtttggcc tcattgatga agaccaaatt gttggtaaag
    10561 tttcatttag attctggcca tttagtgaat ttaaacataa tttcaatcct gaaaatacta
    10621 aaaattaata tgaaacaaat acaacatcgt ttgtcggttt taatactgat aaacgatgtt
    10681 ttattttgtt agtaccacaa taaaagctaa gttcgaaatg aacttataat aaatcaatca
    10741 caatcacttt gtgttaaaat atgtgtcaaa ggaagtgagg gtttgtcatg acattacatg
    10801 cttatttagg tagagcggga acaggtaagt ctacgaaaat gttgaccgaa ataaaacaaa
    10861 aaatgaaagc agatccgctt ggagatccaa tcattttaat tgcgccaact caaagtacat
    10921 ttcaattaga acaagccttt gtcaatgatc cggaattaaa tggtagttta agaacagaag
    10981 tgttgcattt tgaacgatta agtcatcgta ttttccaaga agttggtagt tatagcgaac
    11041 aaaagttatc taaagctgca acggaaatga tgatttataa cattgttcaa gaacaacaaa
    11101 agtatttaaa actttatcaa tcacaagcaa aatattatgg gtttagtgaa aaattaacag
    11161 aacaaattca agattttaaa aaatatgcag taacgcctga acatttagaa cactttattg
    11221 ctgataaaaa tatgcaaact cgaactaaaa ataagttaga ggatattgct ttaatatacc
    11281 gtgagttcga acaacgcatt caaaacgagt ttattacggg tgaggattca ttacaatatt
    11341 ttattgattg tatgccgaaa tcagagtggc taaaacgtgc tgatatatat attgatggtt
    11401 ttcacaactt ttcaacgatt gagtatttaa taatcaaagg attaattaaa tatgcgaaga
    11461 gtgtcacaat tatattgacg acagatggta accacgatca atttagttta tttagaaaac
    11521 catcggaagt gttacgacat attgaagaaa tagcaaatga actcaatatt tctattgaac
    11581 gtcaatattt caagcaatta tatcgcttca ataatcaaga tttaaagcat cttgaacaag
    11641 aatttgatgc acttcaaatc aatcgagtgg catgtcaagg tcatatcaat attttagaat
    11701 ctgcgactat gagagaggaa ataaatgaaa ttgcgcgacg tatcatcgtt gatattcgtg
    11761 ataagcaatt acgatatcaa gatattgcaa ttttatatcg tgacgagtct tatgcttatt
    11821 tatttgattc catattaccg ctttataata ttccttataa cattgataca aagcgttcga
    11881 tgacacatca tccggtcatg gaaatgattc gttcattgat tgaagttatt caatctaatt
    11941 ggcaagtgaa tccaatgcta cgcttattga agactgatgt gttaacggca tcatatctaa
    12001 aaagtgcata cttagttgat ttacttgaaa attttgtact tgaacgtggt atatacggta
    12061 aacgttggtt agatgatgag ctatttaatg tcgaacattt tagcaaaatg gggcgtaaag
    12121 cgcataaact gaccgaagat gaacgtaaca catttgaaca agtcgttaag ttaaagaaag
    12181 atgtcattga taaaatttta cattttgaaa agcaaatgtc acaagcggaa actgtaaaag
    12241 actttgcaac tgctttttat gaaagtatgg aatatttcga actgccaaat caattgatga
    12301 cagagcgaga tgaacttgat ttaaatggta atcatgaaaa ggcggaggaa attgatcaaa
    12361 tatggaatgg cttaattcaa atccttgacg acttagttct agtatttgga gatgaaccaa
    12421 tgtcgatgga acgtttttta gaagtatttg atattggttt agaacaatta gaatttgtta
    12481 tgattccgca aacattggac caagttagta ttggtacgat ggatttggct aaagtcgaca
    12541 ataagcaaca tgtttactta gttggaatga atgatggtac gatgccacaa ccagtaactg
    12601 cgtcaagctt gattacagat gaagaaaaga aatactttga acagcaggct aatgtcgagt
    12661 taagtccaac atcagatatt ttacagatgg atgaagcatt tgtttgttat gttgctatga
    12721 ctagagctaa gggagatgtt acattttctt acagtctaat gggatcaagt ggtgatgata
    12781 aggagatcag cccattttta aatcaaattc aatcattgtt caaccaattg gaaattacta
    12841 acattcctca ataccatgaa gttaacccat tgtcactaat gcaacatgct aagcaaacca
    12901 aaattacatt atttgaagca ttgcgtgctt ggttatatga tgaaattgtg gctgatagtt
    12961 ggttagatgc ttatcaagta attagagata gcgatcattt aaatcaaggt ttagattatt
    13021 taatgtcagc attaacgttt gacaatgaaa ctgtaaaatt aggtgaaacg ttgtctaaag
    13081 atttatatgg taaggaaatc aatgccagtg tatcccgttt tgaaggttat caacaatgcc
    13141 catttaaaca ctatgcgtca catggtctga aactaaatga gcgaacgaag tatgaacttc
    13201 aaaactttga tttaggtgat attttccatt ctgttttaaa atatatatct gaacgtatta
    13261 atggcgattt taaacaatta gacctgaaaa aaataagaca attaacgaat gaagcattgg
    13321 aagaaatttt acctaaagtt cagtttaatt tattaaattc ttcagcttac tatcgttatt
    13381 tatcaagacg cattggcgct attgtagaaa caacactaag cgcattaaaa tatcaaggca
    13441 cgtattcaaa gtttatgcca aaacattttg agacaagttt tagaaggaaa ccaagaacaa
    13501 atgacgaatt aattgcacaa acattaacga caactcaagg tattccaatt aatattagag
    13561 ggcaaattga ccgtatcgat acgtatacaa agaatgatac aagttttgtt aatatcattg
    13621 actataaatc ctctgaaggt agtgcgacac ttgatttaac gaaagtatat tatggtatgc
    13681 aaatgcaaat gatgacatac atggatatcg ttttacaaaa taaacaacgc cttggattaa
    13741 cagatattgt gaaaccaggt ggattattat acttccatgt acatgaacct agaattaaat
    13801 ttaaatcatg gtctgatatt gatgaagata aactagaaca agatttaatt aaaaagttta
    13861 agttgagtgg tttagttaat gcagaccaaa ctgttattga tgcattggat attcgtttag
    13921 aacctaaatt cacttcagat attgtaccag ttggtttgaa taaagatggc tctttgagta
    13981 aacgaggcag ccaagtggca gatgaagcaa cgatttataa attcatccaa cataacaaag
    14041 agaattttat agaaacagct tcaaatatta tggatggaca tactgaagtt gcaccattaa
    14101 agtacaaaca aaaattgcca tgtgcttttt gtagttatca atcggtatgt catgtagatg
    14161 gcatgattga tagtaagcga tatcgaactg tagatgaaac aataaatcca attgaagcaa
    14221 ttcaaaatat taacattaat gatgaatttg ggggtgagca atagatgaca attccagaga
    14281 aaccacaagg cgtgatttgg actgacgcgc aatggcaaag tatttacgca actggacaag
    14341 atgtacttgt tgcagccgcg gcaggttcag gtaaaacagc tgtactagtt gagcgtatta
    14401 tccaaaagat tttacgtgat ggcattgatg tcgatcgact tttagtcgta acgtttacaa
    14461 acttaagcgc acgtgaaatg aagcatcgtg tagaccaacg tattcaagag gcatcgattg
    14521 ctgatcctgc aaatgcacac ttgaaaaacc aacgcatcaa aattcatcaa gcacaaatat
    14581 ctacactcca tagtttttgc ttgaaattaa ttcaacagca ttatgatgta ttaaatattg
    14641 acccgaactt tagaacaagc agtgaagctg aaaatatttt attattagaa caaacgatag
    14701 atgaggtcat agaacaacat tacgatatcc ttgatcctgc ttttattgaa ttaacagagc
    14761 aattgtcttc agatagaagt gatgatcagt ttcgaatgat tattaaacaa ttgtatttct
    14821 ttagcgttgc aaatccaaat cctacaaatt ggttggatca attggtgaca ccatacgaag
    14881 aagaagcaca acaagcgcaa cttattcaac tactaacaga cttatctaaa gtatttatca
    14941 cagctgctta tgatgcttta aataaggcgt atgatttgtt tagtatgatg gatagcgtcg
    15001 ataaacattt agctgttata gaagatgaac gacgtttaat ggggcgtgtt ttagaaggtg
    15061 gctttattga tataccttat ttaactggtc acgaatttgg cgcgcgtttg cctaatgtaa
    15121 cagcgaaaat taaagaagca aatgaaatga tggtcgatgc cttagaagat gctaaacttc
    15181 agtataaaaa atataaatca ttaattgata aagtgaagag tgattacttt tcaagagaag
    15241 ctgatgattt gaaagctgat atgcaacaat tggcgccacg agtaaagtac cttgcgcgta
    15301 ttgtgaaaga tgttatgtca gaattcaatc gaaaaaagcg tagcaaaaat attttggatt
    15361 tttctgatta tgaacatttt gcattacaaa ttttaactaa tgaggatggt tcgccttcag
    15421 aaattgccga atcataccgt caacacttcc aagaaatatt ggtcgatgag tatcaagata
    15481 cgaaccgagt tcaagagaaa atactatctt gcatcaaaac gggtgatgaa cataatggta
    15541 atttatttat ggttggagat gttaagcaat ccatttataa atttagacaa gctgatccaa
    15601 gtttatttat tgaaaagtat caacgcttta ctatagatgg agatggcact ggacgtcgaa
    15661 ttgatttgtc gcaaaacttc cgttctcgaa aagaagtact gtcaacgact aactatatat
    15721 tcaaacatat gatggatgaa caagtcggtg aagtaaaata tgatgaagcg gcacagttgt
    15781 attatggtgc accatatgat gaatcggacc atccagtaaa cttaaaagtc cttgttgaag
    15841 cggatcaaga acatagtgat ttaactggta gtgaacaaga agcgcatttt atagtagaac
    15901 aagttaaaga tatcttagaa catcaaaaag tttatgatat gaaaacagga agctatagaa
    15961 gtgcgacata caaggatatc gttattctag aacgcagctt tggacaagct cgcaatttac
    16021 aacaagcctt taaaaatgaa gatattccat tccatgtgaa tagtcgtgaa ggttactttg
    16081 aacaaacaga agtccgctta gtattatcat ttttaagagc gatagataat ccattacaag
    16141 atatttattt agttgggtta atgcgctccg ttatatatca gttcaaagaa gacgaattag
    16201 ctcaaattag aatattgagt ccaaatgatg actacttcta tcaatcgatt gtaaattaca
    16261 ttaatgacga agcagcagat gcaattttag ttgataaatt aaaaatgttt ttatcagata
    16321 ttcaaagtta ccaacaatat agtaaagatc atccggtgta tcagttaatt gataaatttt
    16381 ataatgatca ttatgttatt caatacttta gtggacttat tggtggacgt ggacgacgtg
    16441 caaatcttta tggtttattt aataaagcta tcgagtttga gaattcaagt tttagaggtt
    16501 tatatcaatt tattcgtttt atcgatgaat tgattgaaag aggcaaagat tttggtgagg
    16561 aaaatgtagt tggtccaaac gataatgtcg ttagaatgat gacaattcat agtagtaaag
    16621 gtctagagtt tccatttgtc atttattctg gattgtcaaa agattttaat aaacgtgatt
    16681 tgaaacaacc agttatttta aatcagcaat ttggtctcgg aatggattat tttgatgtgg
    16741 ataaagaaat ggcatttcca tctttagctt cggttgcata tagagctgtt gccgaaaaag
    16801 aacttgtgtc agaagaaatg cgattagtct atgtagcatt aacaagagcg aaagaacaac
    16861 tttatttaat tggtagagtg aaaaatgata aatcattact agaactagag caattgtcta
    16921 tttctggtga gcacattgct gtcaatgaac gattaacttc accaaatccg ttccatctta
    16981 tttatagtat tttatctaaa catcaatctg cgtcaattcc agatgattta aaatttgaaa
    17041 aagatatagc acaaattgaa gatagtagtc gtccgaatgt aaatatttca attgtgtact
    17101 ttgaagatgt gtctacagaa accattttag ataatgatga atatcgttcg gttaatcaat
    17161 tagaaactat gcaaaatggt aatgaagatg ttaaagcaca aattaaacac caacttgatt
    17221 atcgatatcc atatgtaaat gatactaaaa agccctcaaa acaatctgtt tctgaattga
    17281 aaagacaata tgaaacagaa gaaagtggca caagttacga acgagtaagg caatatcgta
    17341 tcggtttttc aacgtatgaa cgacctaaat ttctaagtga acaaggtaaa cgaaaagcga
    17401 atgaaattgg tacgttaatg catacagtga tgcaacattt accattcaaa aaagagcgca
    17461 tatctgaagt tgagttacat cagtatatcg atggattaat cgataaacat attatcgaag
    17521 cagatgcgaa aaaagatatc cgtatggatg aaataatgac atttatcaat agtgagttat
    17581 attcgattat tgctgaagca gagcaagttt atcgtgaatt accgtttgta gttaaccaag
    17641 cattagttga ccaattgcca caaggagacg aagacgtctc aattattcaa ggtatgattg
    17701 acttgatctt tgttaaagac ggtgtgcatt attttgtaga ctataaaacc gatgcattta
    17761 atcgtcgtcg tgggatgaca gatgaagaaa ttggtacaca attaaaaaat aaatataaga
    17821 tacagatgaa atattatcaa aatacgcttc aaacgattct taataaagaa gttaaaggtt
    17881 atttatactt cttcaaattt ggtacattgc aactgtagta ttttgatttt caaaagaata
    17941 aaaaataatt tcgattaagt gcaaagccct tgtagcagaa tgaacataac tcattttcaa
    18001 aattgtctta cttatttatt tgttatttga taacgaaaaa agttataatg tgaattaaga
    18061 taaagatgag gagttgagaa tgaatgaaat tcttatcatt caagtataat gacaaaactt
    18121 catatggcgt taaagtaaaa cgcgaagatg ctgtatggga tttaacacaa gtatttgctg
    18181 actttgcaga aggagatttc catcctaaaa cattgttagc tggtttacaa caaaatcata
    18241 ctttagattt tcaagaacaa gtacgtaaag cagttgtagc agcagaagat agcggcaaag
    18301 ctgaagacta taaaatttca tttaatgaca ttgaattctt accaccagta acacctccga
    18361 ataatgtgat tgcttttggt agaaattaca aagatcatgc gaacgaatta aatcatgaag
    18421 tagaaaaatt atatgtattt acaaaagcag cgtcatcttt aacaggagat aatgcaacaa
    18481 ttccaaatca taaagatatt actgatcaat tagattatga aggtgaatta ggtattgtta
    18541 ttggtaagtc tggtgaaaag attccaaaag cattagcttt agattatgtt tacggctata
    18601 caattattaa cgatatcact gatcgcaaag cacaaagtga acaagatcaa gcatttttat
    18661 caaaaagttt aactggcggt tgcccaatgg gtccttatat cgttactaaa gacgaactac
    18721 cattacctga aaatgtaaat attgttacaa aagttaacaa tgaaattaga caagatggta
    18781 acactggcga aatgattctt aaaattgatg aattaataga agaaatttca aaatatgttg
    18841 cactacatcc gggagatatt attgcaactg gtacaccagc aggcgttggt gcaggtatgc
    18901 aaccacctaa atttttacaa ccaggtgatg aagttaaagt gactattgat aatattggaa
    18961 cactgacaac ttatatcgct aaataattat catttaaaaa gcgaggctgg ga
//
LOCUS       DAHHYJ010000038        18960 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000038 DAHHYJ010000000
VERSION     DAHHYJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18960
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.038"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(143..607)
                     /gene="bstA"
                     /locus_tag="NBK22_001004"
     CDS             complement(143..607)
                     /gene="bstA"
                     /locus_tag="NBK22_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCD2414202.1"
                     /translation="MTTKTVFDVIDMGLGYLVNVYDTWKVEKILDDYHKPFSNTIHWQ
                     FGHVLTIFESALAVTGKENIDLNIYRPLFGNGSSPDEWKGEVPSIGTILEGLQTLPER
                     ARKLTEDDLAIELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            complement(711..1349)
                     /gene="pcp"
                     /locus_tag="NBK22_001005"
     CDS             complement(711..1349)
                     /gene="pcp"
                     /locus_tag="NBK22_001005"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCD2414203.1"
                     /translation="MHILVTGFAPFDNQNINPSWEAVTQLEDIIGTHTIDKLKLPTSF
                     KKVDNIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARIPDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTYVCNHVLYHLGYLQDKHYPHLR
                     FGFIHVPYIPEQIGGKPDTPSIPLEKIVAGLTAAIEAISNDEDLRIALGTTE"
     gene            complement(1501..2457)
                     /locus_tag="NBK22_001006"
     CDS             complement(1501..2457)
                     /locus_tag="NBK22_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCD2414204.1"
                     /translation="MNYQVLLYYKYTPIDDPAQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDETEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENETK
                     YLGACSYECAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            2701..3672
                     /locus_tag="NBK22_001007"
     CDS             2701..3672
                     /locus_tag="NBK22_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCD2414205.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQTITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKINPETKEIKQPFVSHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFAATENGDNLQDIIEDLSTAYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVSPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(4150..4665)
                     /locus_tag="NBK22_001008"
     CDS             complement(4150..4665)
                     /locus_tag="NBK22_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCD2414206.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATATIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSVSESKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGVIVTGTINRENYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            5045..5893
                     /locus_tag="NBK22_001009"
     CDS             5045..5893
                     /locus_tag="NBK22_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCD2414207.1"
                     /translation="MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPY
                     DIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTFSGHYIITPDNGSLSHI
                     KHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVD
                     SIEALEIREATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQ
                     DKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHL
                     SKAPSI"
     gene            5922..6476
                     /locus_tag="NBK22_001010"
     CDS             5922..6476
                     /locus_tag="NBK22_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HCD2414208.1"
                     /translation="MKKQDISVKTVVAIGIGAAVFVILGRFVVIPTGFPNTNIETSYA
                     FLALISAIFGPFAGLMTGLVGHAIKDFTTYGSAWWSWVICSGIIGCLYGWIGLKLNLS
                     SGRFSRKSMIYFNIGQIIANIICWALIAPTLDILIYNEPANKVYTQGVISAVLNIISV
                     GIIGTILLKAYASSQIKKGSLRKE"
     gene            6529..8241
                     /locus_tag="NBK22_001011"
     CDS             6529..8241
                     /locus_tag="NBK22_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414209.1"
                     /translation="MTEPIISFKDFSFQYHSQATPTLQNINVDIYPGEKVLVVGASGS
                     GKSTFANCINGLIPFKTKGNITGELYINNQDATVSCLHDRSNVVGTVLQDTDGQFIGL
                     TAAEDMAFLLENNCVEQDDMKKNVSYWAEKVGMIEHLNHRPQDLSGGQKQRVSLGGIL
                     IHRTPILILDEPLANLDPATGHETLRLLNNIHEETKSTMIIVEHRLEESLDDTFDRVL
                     LFKDGKIIANTTPSDLLKSSKLKEAGIREPLYCTALKYAEVDVESIDNLANLRDVCMS
                     EHVKFKVKKWIDETSANNDNKYKSEPLLELNEVCVQYSDYSNSVLNNVQLNVYRREML
                     SIVGHNGAGKSTLAKAICGFLDITGNIQFCNRGFNQLSISERSEFVGYVMQNPNHMIS
                     EKMIYDEVALGLRARGMKESDIKIRVENVLKICGLYAFRNWPIVALSYGQKKRVTIAS
                     VLVLNPEIIILDEPTAGQDFYHYNEIMSFLIELNRQGKTIIMITHDMHLLSEYSSRTI
                     VLSKGQVVADTTPVLVLNDKKICEIASLRQTSLFEMAEYIGISEPQKLVQLFINHDRK
                     VRRQ"
     gene            8238..9071
                     /locus_tag="NBK22_001012"
     CDS             8238..9071
                     /locus_tag="NBK22_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD2414210.1"
                     /translation="MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYL
                     ILISASSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVRIYNQRTELV
                     NGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYA
                     VSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERID
                     TISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPW
                     H"
     gene            9118..9614
                     /locus_tag="NBK22_001013"
                     /pseudo
     CDS             9118..9614
                     /locus_tag="NBK22_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9732..10856
                     /locus_tag="NBK22_001014"
     CDS             9732..10856
                     /locus_tag="NBK22_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCD2414211.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKSVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDKKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAAEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRHTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
     gene            11160..11978
                     /locus_tag="NBK22_001015"
     CDS             11160..11978
                     /locus_tag="NBK22_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCD2414212.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHK
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            11996..12610
                     /locus_tag="NBK22_001016"
     CDS             11996..12610
                     /locus_tag="NBK22_001016"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCD2414213.1"
                     /translation="MLRIAIAKGRLMDSLINYLDVIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            12603..13853
                     /locus_tag="NBK22_001017"
     CDS             12603..13853
                     /locus_tag="NBK22_001017"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCD2414214.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVDQVFQVGGAQSIAALTYGTETIPKVDKVVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDETADLDAIVYDVFAQAEHDELARTYAISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            13869..14883
                     /locus_tag="NBK22_001018"
                     /pseudo
     CDS             13869..14883
                     /locus_tag="NBK22_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
     gene            14852..15430
                     /gene="hisB"
                     /locus_tag="NBK22_001019"
     CDS             14852..15430
                     /gene="hisB"
                     /locus_tag="NBK22_001019"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCD2414215.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGTMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRALGIALTATDDQRVPSSKGVIE"
     gene            15427..16005
                     /gene="hisH"
                     /locus_tag="NBK22_001020"
     CDS             15427..16005
                     /gene="hisH"
                     /locus_tag="NBK22_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCD2414216.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNTSKIIDQAETIILP
                     GVGHFKDAMSEIKRLNLNAILAKNTDKKMIGICLGMQLMYEHSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGADIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            15998..16702
                     /locus_tag="NBK22_001021"
     CDS             15998..16702
                     /locus_tag="NBK22_001021"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCD2414217.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSQFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKVMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRQLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            16699..17457
                     /gene="hisF"
                     /locus_tag="NBK22_001022"
     CDS             16699..17457
                     /gene="hisF"
                     /locus_tag="NBK22_001022"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCD2414218.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLANIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            17454..18086
                     /locus_tag="NBK22_001023"
     CDS             17454..18086
                     /locus_tag="NBK22_001023"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCD2414219.1"
                     /translation="MTKYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQRVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
BASE COUNT         6647 a         2803 c         3499 g         6011 t
ORIGIN      
        1 aattaacgaa atcattaata taattatttt tcgagaatcg gtgaagaact ggtatagatg
       61 gtgtttatta aattcaaaaa agtgaccttt taaacaaata aaaaacccgt taacgtgttg
      121 ttaacggtta ataagcacat atttatttta aattttttaa tatacgagac atctcttcaa
      181 ttttaccagc atgaagtggg atgtgaatgg catttaatac taataactct tctaagttat
      241 tacaaccaac aattggttgt ttcaattcaa ttgctaaatc atcttcagtt agttttcgtg
      301 cacgttcagg taatgtttga agaccttcta aaatcgttcc aatactcggt acttcgccct
      361 tccattcatc tggagacgaa ccatttccga ataaaggtct atatatattt aaatcaatat
      421 tctctttacc agtaacagct aaggctgatt caaaaattgt taatacatgc ccaaattgcc
      481 aatgaatggt attagaaaaa ggcttatgat aatcatcaag tatcttttca actttccaag
      541 tatcatacac atttactaaa tatcctaaac ccatatcaat gacatcaaat accgtttttg
      601 tagtcattat taattcctcc ccccataaat aaaacatact tccgtaagta ttaccctatt
      661 aacaaagttg ttagtcattt aaggaatgta ttttgttgtt actcttataa ctattccgtt
      721 gtgcctagag ctatacgtaa atcctcatca ttagaaatag cttcaatggc tgcagtcaaa
      781 cctgcaacta tcttttctaa tggtatagat ggtgtatcgg gtttaccacc aatctgctct
      841 ggtatgtatg gcacatgaat aaatccgaat cgtaggtgag ggtaatgctt atcttgtaaa
      901 taacctaagt gataaagcac gtgattacat acatacgtac ctgcgctatt tgaaagtgct
      961 ccaggaagtc cttgattaat aatactttga gtcattgctt taactggtaa atttgaaaaa
     1021 taagctggcg caccgtctaa gtgaatggct tgatcaatag gttgaaaatc atcattatct
     1081 ggaatacgtg catcatcaat attaatggcg acacgttctg gggtaatggc atttctacca
     1141 ccagcttgtc ctatagctag tacaacatca tagtgattag atgccaacgt tttatttata
     1201 atattatcta ctttcttaaa agaggttggt agttttaatt tatcgattgt atgtgtgcca
     1261 ataatatctt ctagttgagt cacagcttcc catgagggat taatattttg attgtcaaaa
     1321 ggcgcaaacc ctgttactaa aatgtgcatt ttaagacacc ccgcagtatt aaaatttaat
     1381 ttagtataac acgcgcataa ttaaaaacat atcaggttat gtatgcgcat aaaaaacagc
     1441 cttgaaaaac ctttataaat cggattttca aagcgtgttt tgaaaggtat ttaattaaaa
     1501 ctaagcatgt tgatgtaaat catcaaagtt tgttaaacgt tgttgccact cattatcact
     1561 aatattattt gcttgaacat aacgattacg ctcatgttta gcacattcat aagagcatgc
     1621 acctaaatat ttagtttcgt tttcttcaga aactaatatt tgtttattac attctgggtt
     1681 agcgcaatta atataacgtt cacatggttt gccatcaaac caatccttac caataattgt
     1741 tttttcaact tggttgatat caacactgat acggtcatca aatacgtaca ttttaccgtc
     1801 ccaatattca ccttttgttt caggatcttt accatatgta gcaataccgc catgaagttg
     1861 agctacatct tcgaaacctt cttttaaaag ccatccagaa aatttttcac atcgaatgcc
     1921 accagtacag tacgtaacca cttttttatc tgcaaataac gctttattct ctttaatcca
     1981 gtcaggcaaa tctctaaagc gcgtgatatt tggacgaatt gcacctcgga aatgacctaa
     2041 atcaaattca taatcattac gtgcatcaat aatgactgtg tcatcatctt caagagcttt
     2101 tctaaattct acaggtgata aatattggcc agttgtgtgt cttggatcga cgtcatcttc
     2161 taaatctaaa gcaacgattt cttttcgagg acgtacatgc attttcttaa aagcatgtcc
     2221 ttcagtttca tcaattttaa acaccatatc tttgaatcgt tcatcggcat gcatatgtgc
     2281 catatattgt tcggtttctt cttttgtacc agataatgtg ccgttaatac cttctgtaga
     2341 aacaagaatt ctacctttta aatggtgtgc tttgcaaaac gctaagtgat cctgagcaaa
     2401 ctgtgcaggg tcatcaatcg gcgtatattt ataatataaa agaacttgat agttcatagg
     2461 ttaatctcct tctaaaaaca tgttataagc atgttaattt ttaaaataaa gcgtcaatac
     2521 gcatatttgt ctacgtccat cattttaaca gagttgtatt gtcattcaat atttctagct
     2581 tgaaatcagc cactagaccg ataaaataga taaatgaaac cgttatcatt ttatttgata
     2641 gttatcttgt ttaaaatgaa aatgatagtt aaaacgaatg aaatgaaagg aatgatcatg
     2701 atgtcacaac aagatttacc tacattattt tatagcggga agtccaatag tgctgttcca
     2761 attatatctg aaagtgaatt acaaacaatt acagctgaac catggcttga aatttccaaa
     2821 aaaggattgc aactagaagg attgaacttt gatcggcagg gacaactctt tttattggat
     2881 gtattcgaag gcaatatttt caaaatcaat cctgaaacga aggaaatcaa acaacctttt
     2941 gtaagtcaca aagcgaatcc tgcagcaatc aaaatacata aagatggccg attattcgtt
     3001 tgttatttag gagattttaa atctacagga ggcatttttg cagctacaga aaatggtgac
     3061 aacttacaag atattattga agatctttca acagcatatt gtattgatga catggtattt
     3121 gattctaaag gtggatttta ttttacagat tttagaggat actctaccaa tccactagga
     3181 ggcgtttatt atgtttcgcc ggactttaga acagtgacgc ctatcattca aaatattagc
     3241 gtagcaaatg gtattgcttt aagtacagat gaaaaagtac tatgggtaac agaaactaca
     3301 gccaatcgat tacatcgcat tgcacttgaa gatgatggtg tgacgataca accatttgga
     3361 gctactatac cgtactattt tacaggtcat gaaggaccag actcatgttg tattgatagt
     3421 gacgataatt tatacgtagc aatgtatggt caaggtcgag tgttagtttt taataaaagg
     3481 ggttatccaa taggacaaat attgatacca ggccgagatg aagggcatat gttacgttct
     3541 actcatccgc aatttatacc tggaacaaat caactcatca tttgttccaa tgatatagaa
     3601 atgggcggag gatctatgct ttatacagtt aatggctttg caaaaggtca tcaaagtttt
     3661 cagtttcaat aagacctacc tgaatgaaag gcgtcttaat ataagtttaa atcttagcga
     3721 tttttaagtt agaagttaaa tataatttgc ttcaatttgt aaaatgaagt tttgtataac
     3781 gtaaataggc aatatttaat ataagaaagt ataaagaaga attttcttga ttatttctaa
     3841 tgaagccaat gccaatttaa taaggagttg tttattttgg ctatttatat tttttgtaca
     3901 atactagttt tagttgcagt tggagcactt ttgacaaata gaataaggga taaaaaagat
     3961 aaaaggctag atattttatc tagtgtatta cttttaattt catcaatatc attgttactt
     4021 tatggtgtta ttatcaatta agttaaatat gatttttaat ttagaagtta aaaaatgcca
     4081 actataccta gggatgatat agttggcatt tcgtatatat attaaaacaa tgaggattgt
     4141 gtaatgagat tattctgaga ttgagaattc agctgatgct tcgaatttaa catctttgcc
     4201 tagcattacg ccaccagttt caagtgcttg gttaaagtta atgccgtagt tttctctatt
     4261 gattgtacca gtaacaataa cacctgttac ttgagaacca tccattggat ttttacttac
     4321 tccgttgaat tcaacatcga atgtttcttc gttagtgatg cctttaattg ttaaatcacc
     4381 aacaactttg ctttcagata ctgattttgt cacaaatgta attttatcaa attcgtcagt
     4441 accaaagaaa tcaccagatt ttaagtggtt atcacgtgct tcgtttttag tgttaattga
     4501 gcttggaata attgttgcag tagctttcaa agtactgaag tcattaatat ctccttcaac
     4561 agctacatca aattgatcaa atgaaccttt cactttagaa accattaaat gtttaatttg
     4621 gaattctaaa ctactgtgtg caccatcaaa agtaaagtta gtcataatat aaaacctcca
     4681 tatttttatc tcttagatat attttttata cttgatatac atacgatacc acctaaaaaa
     4741 caaagatgca agcattttat ttcgaattcg aatattttct gtgatgtatt taaagaatga
     4801 cctataaaac accacactat tgttataccc tagtcaatta attcaaacat aaagaagtga
     4861 ttacaagtcc gtgaaattta gttaatgaga taaatctgac tattattatc atttttatag
     4921 tcactactat tgactttgca tcaagtgact cgtactattt taatataatt ctgaaaattc
     4981 aaaatataga acattaatta tgtagataat atttttttga agttttaaaa aaaggaggca
     5041 ttttatggga aattatttgg tattgcaatc agattttgga cttagtgatg gcgctgtaag
     5101 tgcaatgtat ggagttgcat atactgtaag tgatgatata cgtgttgaaa acttaacgca
     5161 tgatatacca ccgtatgaca tttgggtagc gtcatatcgt ttatatcaaa ctgtaaaata
     5221 ctggcctaaa ggcacagttt ttgtatcagt ggtagacccg ggtgtaggta gtgataggcg
     5281 gagcattgct tgcttaacat tttcaggtca ttacattatt acacctgata atggttcttt
     5341 aagtcatatt aaacattacg aaggtattaa aaaagttatc gaaattgatg aagtgaaaag
     5401 ccgattgcct cattcggaag aaagtcatac ttttcatggt agagatgttt atgcatacaa
     5461 tggtgcgaga ttagctgcaa ataagatagc gtttgaacgt ctcggtcagg caattaatgt
     5521 tgatagtatt gaggcattag aaataagaga agcgacaaaa aatgaggatt ctgttacagg
     5581 aagcattgat attttagata tcagatttgg gtcattatgg acaaatattc cattggcatt
     5641 cttaaaatcc ttggaaattg ttcatggtaa taatttggtg gtaacaattt atcatcaaga
     5701 taagaaagtc tatcaaaata ttattaaatt tgcgcgatcg tttgcagatg ttaatgtggg
     5761 tgaacctctt gtgtatgtta actcgctagt aaatattggt gtagcggtca atcaagactc
     5821 attttcagat ttatatcata ttggtactgg aaatgattgg acgattcatt tatctaaagc
     5881 accatcaata taatgaattt tttatgtaag gagtaatgaa gatgaaaaaa caagatattt
     5941 cagttaaaac tgttgttgca attggtatag gagcagcggt atttgtaatt ttagggcgtt
     6001 ttgttgtaat accaacaggt tttcctaata cgaatataga aacatcttat gcatttttag
     6061 cattaatatc tgcaatattt gggccttttg ctggtttaat gactggatta gttggacacg
     6121 ctattaaaga tttcacgaca tacggtagtg cttggtggag ttgggttatt tgttcaggaa
     6181 ttataggctg tttatatgga tggattggcc taaaattaaa tctttcttca ggccgatttt
     6241 ctaggaaatc gatgatttat tttaatatcg gtcaaattat tgcgaatatt atttgctggg
     6301 cacttattgc accaacatta gatattttga tttataacga accggctaac aaggtttata
     6361 cacaaggtgt tatctctgca gtattaaata ttatttcagt tggtattatt gggacaatat
     6421 tattaaaagc atatgcttca tctcaaataa aaaaaggtag tttacgtaaa gaataatcat
     6481 tttgttgaat cagatatgta aatgaatgta gaaaggtaat gatatatcat gactgaacca
     6541 attatctcgt tcaaagactt tagttttcaa tatcatagtc aagcaacacc tacattacag
     6601 aatataaatg ttgatattta tccaggagaa aaagtgctag tagttggtgc ttcaggtagt
     6661 ggtaaatcga cttttgcaaa ttgcataaac ggattaattc catttaaaac taaaggtaac
     6721 ataactgggg aactatatat aaataatcaa gatgcaaccg ttagttgttt acatgataga
     6781 tctaatgttg ttggtacagt tttacaagat acagatggac agttcatagg cttaacagca
     6841 gctgaagata tggccttttt attagaaaat aattgtgttg aacaagatga tatgaagaaa
     6901 aatgtaagtt attgggctga aaaagttggc atgatagaac atttaaatca ccgaccgcaa
     6961 gatttatctg gaggtcaaaa acaacgcgtt tcattaggtg gtatattaat ccatcgtacg
     7021 cctattttaa tattggatga gccactggcc aatttagatc ctgcgacagg acatgaaacg
     7081 ctgagattgt taaacaatat tcatgaagaa acaaagtcaa cgatgattat tgtcgaacat
     7141 cgattagaag aatcattaga tgatacgttt gatagagttc ttctttttaa agatggaaaa
     7201 atcatcgcta atacaacgcc tagtgatttg ctaaaatcat ctaagcttaa agaagcaggc
     7261 atacgtgaac cattatactg tacagcgctt aaatatgcag aagtagatgt cgaatccatt
     7321 gataacttag cgaatttacg tgacgtttgt atgagtgaac atgttaaatt taaagtgaaa
     7381 aaatggatag atgaaacatc tgcaaataat gataataagt acaaatctga accgctatta
     7441 gaattaaatg aggtatgtgt tcaatatagt gactatagta atagtgtact taataatgtt
     7501 caattaaatg tttatagaag agaaatgctt agtatagttg gtcacaatgg tgccggcaaa
     7561 tcaacacttg ctaaagcaat ttgtggattt ttagatataa caggtaatat ccaattttgt
     7621 aataggggat ttaatcaatt gtcaattagc gaacgatctg aattcgtagg ttatgtgatg
     7681 caaaatccaa atcatatgat ttctgaaaaa atgatttacg atgaagtagc attagggtta
     7741 agagcacgag gtatgaaaga atcagatata aagatacgag tagagaatgt gctcaaaata
     7801 tgcggacttt atgcatttcg gaattggcca attgtagctt taagctatgg tcaaaaaaag
     7861 agggtcacta tagcatctgt tttagtctta aatccggaaa taatcatatt ggatgaaccg
     7921 actgctggtc aagatttcta tcattataat gagataatgt catttttaat tgagctaaac
     7981 agacagggga agacgattat tatgattacg catgatatgc atttattgtc tgagtatagt
     8041 tcaagaacaa ttgtattatc aaaaggacaa gtcgttgctg ataccacgcc agtattggta
     8101 ttaaatgata aaaaaatctg tgagattgca tcattgagac aaacatcgct atttgaaatg
     8161 gccgaatata tagggattag cgagccacag aaattagtac aattatttat taaccatgat
     8221 aggaaggtga gacgccaatg aatcaatata atactatagg ttttcacccg ggaaatagtc
     8281 gtattcatca attaaatgcg actgttaaac ttttattctt attagttgtt tctatttctg
     8341 caatggtgac ttatgacaca agatatttaa ttttaattag tgcttcatct attttattgt
     8401 tcaaatatgc tcatattgaa tggaaacaag ttcgctttgt tgttaaattc attctgtttt
     8461 tcacaatatt aaatattatt gccgtataca tatttgaccc tgaatatggt gtgaggattt
     8521 ataatcagcg tacagagtta gtcaatggta ttggtcgatt tacgctaaca tcacaggaat
     8581 tattctatct ttttaatcta atattaaaat atattagcac agttccttta gcgttaatat
     8641 ttttattcac aacgaatccg agtcattttg ctgcaagttt aaatcagcta ggtgtgaatt
     8701 ataaaatcag ttacgcagtc tcactagcat taaggtatat tccagatatt caagaaacat
     8761 attttaatat ttcacaagcg caacaagcaa gaggatatga taattcgaaa aaagcgaaat
     8821 ttactagccg tgttaaaggt atcaaacgca ttgtgttacc tttaatattt tctagtatcg
     8881 aaagaattga cactattagt actgctatgg agttaagaca attcggacag tataaaagga
     8941 gaacctggta cgtcaaaaaa caattaaaaa aagatgatta tgttgttttg tgtttgacgt
     9001 taatacttct gatgttagta gttacattat tctttttaaa taatagtcga tatttcaacc
     9061 cgtggcatta gtattcatat aaatagtctt taaatagaaa taggagggag acatttaatg
     9121 ataaatactg aaagattaaa tttaatgatt ccaagttcct cgcatttaat tgaactttat
     9181 aatatttgta gtcatccaca agcaaatata tacactccca aaggtttaca taattccaaa
     9241 ttagacacac aacggtggat tgaaaaatgg cgaaaccatt ggcaacaata tcaatttggt
     9301 tactttgtat tggtaaaaaa atagattgta gtgttattgg tatttgtgga tatgaatatc
     9361 gacaattaaa gcaagaaaca gtattaaatt tattttataa attacatcca agttttgaag
     9421 gacaagggta cgcatgtgag gctattacag caatcacaaa ttttgtgaat tatatcgatc
     9481 aagaaacagt aaaagttatc aggacaaata ggtgtaacca acgttcaata aatttagcag
     9541 aaaggcttaa attcaagcga gacaaagcta tggacgacat tatcaatcaa ggagatattg
     9601 tgttttataa ataaaacact atgacattat ctaaaaaata aaattaaaat cccaacagtt
     9661 tcttaaaaag ttatgagcca cataataaaa atgttataat tgtgcataat aaaacatagg
     9721 gagaatgttt aatgcataaa aaatatttat tgctattttt tctgttatta tcagttgcat
     9781 tgttatatag ctgttccaag caaaatgaga aagctcaaaa taaatctgtt aatccaaatg
     9841 agtatgcaaa gcataaatcg aaaatggatg aacattggga aacatatcac ggccaagttt
     9901 atcatgtgtt ttatcaccca ttaattacag acccaaaagt tgcgtttaca ggagatacac
     9961 accaagctaa gggcaacaat gattggatga ttactgtaag tgaatttaaa aaatcattga
    10021 acgaattata taaaaataat tacatcatcg ttaatccaca tgatgtatac gacttatcat
    10081 ctcatcatgt aaaagccaaa aaaataaaat tgccgaaagg taaaaagcca ctaatcatct
    10141 caattgatga tatgaattac tatagttaca tgagacacca tggttatgct gaccgtttag
    10201 tactcgataa aaataaacat gttgtatctg aaacgacaga caaaaaaggc catgctactt
    10261 attctgatga caatgatatc gtgccaattt taaataaatt tgtaaaagag catccagatt
    10321 tttctttaaa tggtgaaaag ggtgttgttg ctttaaccgg ttatgagggc gtattaggtt
    10381 atcgcacgaa tgaattaacg agtaaagatt atacaaagaa taaaaaggca gctgaggaag
    10441 ttgttcgcgc aatgaaacgg gatggatggt catttgcgag tcattcatat ggtcacatta
    10501 attttgaaaa aacatcttta gaaggtatta aacgtgatac taaaagatgg aaagatgaag
    10561 ttgaaccaat tgtaggtaaa acagatatgt ttgtatttcc acatggcgca caggatagac
    10621 atacacaagc gtatgattat ttggtagatg aagcagagtt taagtttatt gcaggtgtcg
    10681 gtccaaacaa ttttacagat atatcagcaa cgaacgttta ccaagatagg gttgcgattg
    10741 atggtctaaa cttatttgaa tttaaatata aactcaaacc gttttttaat cctgaaaatg
    10801 tatatagtaa acaagataga caattcttta aaggtaatcg ggattatgaa gaatagttaa
    10861 atttaataaa aagatagaaa gaaaataact ttacataata aggcaaggac aatatgtgca
    10921 agaagtacaa cttcaatata aaaatcttgg ttaagcattt ttataggcac aaatgtcttg
    10981 cctttttctt ttgtaaaaat ttaaatacaa tacaccaaat tttctgaaaa aggttgtaat
    11041 gtattgctaa aaatgataag ttattaatca tattacactt tagcgtggta tagaagtaaa
    11101 gtgaacaacg aaaataccaa gtgattttaa atacagtatt aaaagtaaag ggtggtcgag
    11161 tgaataattc agaacaatta attgcgttaa aagaatcgga aacagcattt ttaaaatatt
    11221 ttaataaagc ggattatgag ttagttgatt ttagtgtggt tgaaaagttg gactggaaac
    11281 aattgaacca tgaggatttg cagcagatgg gtgagcgaaa tttttggcaa catgagcatc
    11341 aaatttatgc acttcgaaat gattttacag atcaattatt gcgttattac tcaatgtatc
    11401 caactgcagc aacaaaagtg gcatacacag gtttaatcat tcggaataat gaagcggctg
    11461 tacaagttgg tttggaaaac tatgcaccat cattagcgaa tgtacaacag agcttaaagc
    11521 tatttattca gtttattcaa cagcagttga gagacaacgt gcattttgtt gtattgggac
    11581 attatcaatt gcttgatgcc ttactcgata aatcgttaca aacaccagac atactatcaa
    11641 tgattgagga acgtaattta tcgggactgg ttacatattt gtcgactgaa catccaatcg
    11701 tgcaaatctt gaaagagaat acgcaacaac aattaaatgt attggaacac tatattccaa
    11761 atgaccatcc agcactcgtt gaattaaaaa tatgggaacg ttggttacat aaacaaggtt
    11821 acaaagacat ccatttagat attactgcgc agccacctag atcctattac acaggtttat
    11881 ttattcaatg tcattttgct gaaaatgaat ctcgagtttt aacaggtggt tattacaaag
    11941 gaagcatcga agggtttgga ttaggattaa cactttaagt aagggagtat gcacaatgtt
    12001 aagaatcgcc atagccaaag gacgtctaat ggatagttta attaactatt tagatgtaat
    12061 tgaatatacg acattatcag aaacattaaa aaatagagaa cgccaattat tattaagtgt
    12121 agataatatt gaatgcattt tagtaaaagg aagtgacgtg ccaatctatg tggaacaagg
    12181 aatggcagac ataggcattg ttggtagcga catattagat gagcgccaat ataatgttaa
    12241 taatttgttg aatatgcctt ttggagcatg tcattttgcg gttgcagcga aacctgaaac
    12301 gaccaattat cgtaaaatcg caacgagtta tgttcatact gctgaaacat attttaaatc
    12361 aaaaggtatt gatgtcgaat tgattaaatt gaatggctct gttgaattgg cctgtgttgt
    12421 agatatggta gacggaattg tcgacatcgt tcaaacaggt actacgctaa aagcgaacgg
    12481 actggttgaa aagcaacata ttagtgatat caatgcaaga ttaataacta ataaagcagc
    12541 ttattttaaa aaatcacaat taatagagca atttattcgc tctttggagg tgtctattgc
    12601 caatgcttaa tgcacaacaa tttttaaatc aattttcatt agaagcacca ttagatgagt
    12661 cattgtatcc aattattcgc gatatttgtc aggaagttaa agttcatgga gataaggctt
    12721 tgaaaatgta taatctaaca ttcgatcata cgaaaacaga tcatttagaa attagtcatg
    12781 aacaaattaa agcagcattt gacacattag atgaaaaaac aaaacaagca ttacaacaaa
    12841 gttatgaaag aattaaagca tatcaagaaa gtattaaaca gacgaatcaa cagttagaag
    12901 aatcagtgga gtgttatgaa atataccatc cactagaaag tgtcggtatt tatgtgcctg
    12961 gtggtaaagc aagttatcct tcaacggtat taatgacagc gacgttagca caagtagcag
    13021 gtgtggaaaa tattgtcgtt gtgacaccac ctcaacctaa tggtgtgtct caagaagtat
    13081 tagcagcatg ctatattacg caagttgatc aagtatttca agttggtggt gctcaaagta
    13141 ttgctgcgtt aacttatggc acagaaacga tacctaaagt tgataaggtt gtaggtccag
    13201 gcaatcaatt tgttgcatat gccaagaaat atttatttgg acaagtaggt attgaccaaa
    13261 tagctggacc aacagaaata gcacttatta ttgatgaaac agcagattta gatgccatcg
    13321 tatatgatgt ttttgcacaa gcagaacatg atgaattagc acgtacatat gccattagtg
    13381 aagatgcgca agtccttaaa gatttagaat cacgtattgc taaagcattg cctaatgtgg
    13441 acagatacga cattgtttct aaaagtatcg ctaatcaaca ctaccttatc catgctagta
    13501 attttgatga agcatgccat gtcatgaata caatcgcgcc tgaacatgcg tcgattcaaa
    13561 cagtaaatcc tcaaccatat attgaaaaag tgaaatatgt gggtgcattg tttattggac
    13621 attattcgcc agaggtcata ggagattacg ttgcaggtcc aagtcatgta ttacctacaa
    13681 atagaacagc tagatttacc aatgggttat cggtcaatga tttcttaaca cggaacacgg
    13741 tcatccattt atcaaaagat acgtttgaac aaattgctga ttcagcacaa catattgctc
    13801 atgttgaagc attatacaat caccagcagt ctattttaat acgtcagtct taggggagtg
    13861 taattgaaat gatttatatt gataaaaatg aaagtccagt tacgccgttg gatgaaaaaa
    13921 caatgacgtc tattattagt gcaacgccat ataatttata tcctgatgca gcatatgaac
    13981 aattcaagga agcttatgct aagttttacg gattatcgcc tgaacaaatt attgcaggaa
    14041 atggatctga tgaattgatt caaaagttaa tgctgatcat gccagaaggt ccggcattaa
    14101 cgctaaatcc tgattttttt atgtatcaag catatgcggc acaagtaaat cgtgaaattg
    14161 catttgtaga tgcaggatca gatttaacgt ttgatttgga aaccatttta acgaaaatcg
    14221 atgaagtaca accatcattt tttattatga gtaatccaca taacccttca ggcaagcaat
    14281 ttgatacggc atttttaaca gctattgcag ataagatgaa agcattaaac ggatactttg
    14341 tcattgatga agcatattta gattatggta cggcatatga cgtggaactg gcaccacaca
    14401 tcttaagaat gcgtacatta tcaaaggcgt ttggaattgc cggcttaaga ttaggtgtct
    14461 taattagtac tgctgaaacg ataaagcata ttcaaaaaat agaacatcca tatccattaa
    14521 atgtatttac gctaaatatt gcgacttata ttttttagac atagagaaga gacaagacaa
    14581 tttttaacga tgcaacgaca gttagctgag cagttaaaac aaatatttga tacacatgtt
    14641 gcagataaaa tgtcagtgtt cccatcaaat gctaattttg tacttactaa aggctcagca
    14701 gcgcaacaat taggacaata cgtatatgaa caaggattta aacctcgctt ttatgatgag
    14761 ccggtgatga agggctatgt aagatactca attgcaacag catcacagtt aaagcaatta
    14821 gaagaaattg ttaaagaatg gagtgcaaaa tatgatttat caaaaacaac gaaacacagc
    14881 tgaaacgcaa ctaaatattt caatatctga tgatcagtca ccatcgcata ttaatacagg
    14941 tgtgggcttt ttaaatcata tgttaacctt gtttacattt catagtggtc tgtcattaaa
    15001 cattgaggca caaggtgata ttgacgtaga tgatcaccac gtaactgaag atatcggcat
    15061 tgtcattggc caattgttac ttgaaatgat taaagataaa aagcatttcg ttcgttatgg
    15121 aacgatgtac attccaatgg atgaaacatt agcacgtgtc gttgtggata taagtgggcg
    15181 cccataccta tcattcaatg catcattaag taaagaaaaa gttggtacgt ttgatacgga
    15241 gttagtagaa gaatttttta gagcggtcgt aatcaatgca agattaacaa cgcatattga
    15301 tttaattcgt ggaggtaata cacaccatga aattgaagct atattcaaag cgttttcccg
    15361 tgcattaggc atagcgctaa ctgcaactga tgatcagcgt gtgccgtcat cgaaaggtgt
    15421 gattgaatga ttgtcatcgt tgattatgga ttagggaata ttagtaatgt aaaacgcgct
    15481 attgaacatt tggggtatga ggtggttgtc tcaaatacct caaaaataat cgatcaagca
    15541 gaaacaatca tattgcccgg tgtcggccat tttaaagatg cgatgtcaga gataaaacga
    15601 ttaaatctca atgcaatatt ggctaagaat actgataaga agatgattgg tatttgttta
    15661 ggcatgcaat taatgtatga gcatagtgat gaaggcgatg catctggatt agggtttatc
    15721 ccaggaaata tttcgcgtat ccaaacagaa tacccagtgc cgcacttagg ttggaataat
    15781 ttagtgagta agcatcctat gttaaatcaa gatgtttact tcgtacattc ttaccaagcg
    15841 ccgatgtcag aaaatgtcat tgcatatgcg cagtatgggg ctgatattcc tgcaattgtt
    15901 caatttaaca attatattgg tattcaattc catcctgaaa aaagcggtac atatgggtta
    15961 caaattttgc gtcaggcaat acaaggggga tttataaatg attgaattat ggccagcgat
    16021 tgatttgatt gggtcaacaa gtgtgaggtt aacagagggt aaatatgata gtgaagaaaa
    16081 aatgtcacgc tcggctgaag aaagtattgc ttactatagt caatttgaat gtgtgaatcg
    16141 tattcatatc gtcgacttga taggtgctaa ggcacagcat gcccgagagt ttgattatat
    16201 taagtcattg aggagattaa caaccaaaga tattgaagta ggtggtggca ttcgtacgaa
    16261 gtcacaaatc atggactact ttgccgcagg gattaattat tgcatagttg gaacgaaagg
    16321 cattcaagat actgattggc ttaaagtgat ggcacataca tttccaggtc gtatttatct
    16381 gtcagttgat gcttatggag aagatattaa agtgaacgga tgggaagagg acacagagtt
    16441 aaatttattt agttttgtga gacagttatc ggatatacct cttggcggca ttatatatac
    16501 tgatattgct aaagatggca aaatgtccgg acctaacttt gaattaactg gtcaattagt
    16561 aaaggcaacg acgattcctg tcattgcttc cggtggtatt agacatcagc aagatattca
    16621 acgattagcg tcgctaaatg ttcacgctgc tattatagga aaggctgcac atcaagcatc
    16681 tttttgggag gggctaaaat gattaaaaaa cgtatcattc catgtttaga tgtcaaagat
    16741 ggtcgtgtcg ttaaagggat tcaatttaaa ggattaaggg atattgggaa tcctgttgat
    16801 ttagcaatgt attacaatga agcgggtgct gatgaattag tatttttaga catctctaag
    16861 acggaagagg gtcatagctt aatgctagaa gtgattgaac agacagcgtc acgcttgttt
    16921 atccctctta ctgtaggggg tgggattcaa agtctcgatg atattaccca attgttaaat
    16981 catggtgcag ataaagtatc attaaattca agtgctttaa aaaatccaca gctcattaaa
    17041 caagcgagtg ataaattcgg tagacaatgc atctgtatag caattgatag ctattatgat
    17101 cctgaaagaa aagcacatta ttgttgtacg catggtggta aaaagatgac aaatattaaa
    17161 gtatatgact gggtacagca agtagaacag ttaggtgcag gtgagctcct cgttacaagt
    17221 atgggacatg atggtatgaa gcaaggattt gatattgaac acctagcaaa tattaagtct
    17281 cttgtaaata ttccaatcat tgcttctggt ggtggtggca atgcacaaca ctttgtagaa
    17341 ttatttgatc agacggatgt ttctgcaggt ttagctgcaa gtatattaca tgaccgagaa
    17401 acgacggttc aatctattaa agaagtgata cggcaagggg gtatagcagt aagatgacca
    17461 aatataaaat tgattttagc aaaggtttag tgccagcaat tttacaagat aatcaaacaa
    17521 aacaagtatt gatgttgggc tatatgaatc aagaagcttt tgataaaacg atagaagatg
    17581 gtgtggtatg tttctattcg cggtcgaaac aacggctatg gacaaaaggt gaaacatctg
    17641 gtcatacgca acgtgttaaa gatattcatg tagattgcga caatgacact attttaattg
    17701 atgtcatacc aaatggacca acatgtcata caggcagtca aagttgtttc aacacagaag
    17761 ttccattttc agtgcaaaca ttagcgcaga cagttcaaga tagtgcccaa tccaataatg
    17821 aaaagtcata tacaaaatat ttattaacag aaggtataga aaagattaca aaaaaatacg
    17881 gtgaagaagc ttttgaagtc gtaattgaag caattaaagg tgacaaaaaa gcatttgtaa
    17941 gtgaagtagc agatgaactt tatcatttat ttgtcttgat gcatgcgctt ggcgtcgatt
    18001 tttcagaaat tgaggcggaa ttagcgcgta gacatcataa gcgcaataac tttaaaggtg
    18061 aacgacaaaa tatcgaacag tggtaaagca agtatggact aagatataag gaaaaggatc
    18121 atggcttata cacttacaaa tattgtggaa aacgtgacat tttcaagttt aaaatacgac
    18181 accaacatat tttaactatg aatgctgtga tggtactaaa gttgcgaact cgttatagat
    18241 aagtagtgga taatcacaat acgaaatcaa aaataattat aaaaagtaaa ttgagcaact
    18301 caggaataga tgtcactgtt aaagatgttg aaaagtatat gaatcgatat aatgaagtta
    18361 tgaagggaaa aaatggcgaa aaagctaaag agaatttagt aacctttaat atcatagttg
    18421 tctttacatt ctttgtattt atactgtaaa taaaaatata tctagcctga aatagaaatg
    18481 tcatagccta tttaaaagac aatctccatt agaactaaga tgtgcatccc gaaagttaga
    18541 ctaaaaaact aactttatgg gatgtatttt tatgctaatc atcataaatt cgagattaag
    18601 ttaaaggtag ttcaagagta attaaacaat aaattaaaaa tagtaggata cttactttga
    18661 gggaagaaaa ttaactgtat atatttagtt taggaacaag tattacggtt tatcctgata
    18721 caattattgt ggatgggatg atatttttag gtttaaaata cgacaccagc aaacattata
    18781 actgtgatag ctcataaatc accccataca gctaatctaa aaaaataata catcattgga
    18841 atcaagcccc aagcatgtaa atattaaaaa tcacaaaaga tatatgtaaa aaagttacaa
    18901 tttgcataat taaattgtgt ctaattattg actaattaaa ttttgccaaa tataatatta
//
LOCUS       DAHHYJ010000039        18816 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000039 DAHHYJ010000000
VERSION     DAHHYJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.039"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..1178)
                     /locus_tag="NBK22_001024"
     CDS             complement(<1..1178)
                     /locus_tag="NBK22_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2414220.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPVLYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            1288..2241
                     /locus_tag="NBK22_001025"
     CDS             1288..2241
                     /locus_tag="NBK22_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCD2414221.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2238..2801
                     /locus_tag="NBK22_001026"
     CDS             2238..2801
                     /locus_tag="NBK22_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2414222.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(2924..3325)
                     /locus_tag="NBK22_001027"
     CDS             complement(2924..3325)
                     /locus_tag="NBK22_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2414223.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(3897..4724)
                     /locus_tag="NBK22_001028"
     CDS             complement(3897..4724)
                     /locus_tag="NBK22_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2414224.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQTSRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDTRFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
     gene            complement(4727..4846)
                     /locus_tag="NBK22_001029"
     CDS             complement(4727..4846)
                     /locus_tag="NBK22_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414225.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            4958..5959
                     /locus_tag="NBK22_001030"
     CDS             4958..5959
                     /locus_tag="NBK22_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCD2414226.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            complement(6081..6545)
                     /locus_tag="NBK22_001031"
     CDS             complement(6081..6545)
                     /locus_tag="NBK22_001031"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCD2414227.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(6558..7739)
                     /gene="ribB"
                     /locus_tag="NBK22_001032"
     CDS             complement(6558..7739)
                     /gene="ribB"
                     /locus_tag="NBK22_001032"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCD2414228.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            complement(7750..8382)
                     /locus_tag="NBK22_001033"
     CDS             complement(7750..8382)
                     /locus_tag="NBK22_001033"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCD2414229.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMGGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            complement(8389..9432)
                     /gene="ribD"
                     /locus_tag="NBK22_001034"
     CDS             complement(8389..9432)
                     /gene="ribD"
                     /locus_tag="NBK22_001034"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCD2414230.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKIAKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQAFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTNQTHIEIIYLKSCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     regulatory      complement(9559..9694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(9913..11415)
                     /locus_tag="NBK22_001035"
     CDS             complement(9913..11415)
                     /locus_tag="NBK22_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD2414231.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSEQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDI
                     YNVVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTL
                     TNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     gene            11938..12252
                     /locus_tag="NBK22_001036"
     CDS             11938..12252
                     /locus_tag="NBK22_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD2414232.1"
                     /translation="MTYKELATFLKVLSDSSRLEILDLLSCGELCACDLLAHFQFSQP
                     TLSYHMKALVKSNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            12252..13544
                     /gene="arsB"
                     /locus_tag="NBK22_001037"
     CDS             12252..13544
                     /gene="arsB"
                     /locus_tag="NBK22_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCD2414233.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGITALTGAFIAVITGVVS
                     FSDVFEVTGIVWNATLTFVSVILISLILDKVGLFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAIVAAFFANDGAALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFHVGFVRYFSRMIIPNLFSLLASIIVLWLYFRKAIPKTFDDNNIKHPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGIIITIPVLFITLLGLYLTLIIF"
     gene            complement(13631..14485)
                     /locus_tag="NBK22_001038"
     CDS             complement(13631..14485)
                     /locus_tag="NBK22_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCD2414234.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(14761..14985)
                     /locus_tag="NBK22_001039"
     CDS             complement(14761..14985)
                     /locus_tag="NBK22_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414235.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
     gene            15184..15654
                     /gene="sigS"
                     /locus_tag="NBK22_001040"
     CDS             15184..15654
                     /gene="sigS"
                     /locus_tag="NBK22_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigS"
                     /protein_id="HCD2414236.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRL
                     QDIFKLLNQRERLWLKLYLKGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN"
     gene            15767..16210
                     /locus_tag="NBK22_001041"
     CDS             15767..16210
                     /locus_tag="NBK22_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCD2414237.1"
                     /translation="MQDNSTKYLLYIQTATSNHLETNCVFLHCDYILKVPINKLVSYY
                     AKLHLSSQSVLIETAKNILNINKLVPIYINPKTILFPLKHKRAPIQIYINAHYIVGMT
                     AIENSTLIHFQEGIQLEVDEPFSLVSKKCHESLALKHFIENTISN"
     gene            complement(16197..16640)
                     /locus_tag="NBK22_001042"
     CDS             complement(16197..16640)
                     /locus_tag="NBK22_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414238.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKQALDQRLSSISDDDSVNLNKPV
                     TFANAYLRQHVFRNKETKSYSMFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVSPK
                     VDLSTTVVILTIIGAILLMIVSIYFIKVVTSYFTKKLLEIKFNSK"
     gene            16938..17573
                     /gene="sdpA"
                     /locus_tag="NBK22_001043"
     CDS             16938..17573
                     /gene="sdpA"
                     /locus_tag="NBK22_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpA"
                     /protein_id="HCD2414239.1"
                     /translation="MQKFKDFFYDDLSVTRGNYFLTLMAAFFITIILFIGIGVSEVHL
                     LYSMLIVLVGLILLRLFKINLFSFKKLTLSQVIYIIGGALLIYGLDNLYLYFHDVPAN
                     EQQLEQEIRNTPFYISIFTVTIIPAIVEEIVFRGMIIRVIFRKHLFLGLIVSSLVFAS
                     LHESDTWIGYLPYLYSGLIFGLIYIKTKRLEVVIFMHFLNNLLALLFIIWR"
     gene            complement(18013..18726)
                     /locus_tag="NBK22_001044"
     CDS             complement(18013..18726)
                     /locus_tag="NBK22_001044"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCD2414240.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
BASE COUNT         6222 a         3280 c         2593 g         6721 t
ORIGIN      
        1 gcatctattt ttttaggttg cgtattattc tccttgaaaa ccattaataa tattaatgca
       61 aatacaagta gtagcatcat accgataaac atcatgcgca tattaaacgc atcaactaat
      121 acaccaccaa gaaatggacc aaatgctttt cctactgtag cagctgaatt cacaaaacct
      181 tggtactgtc cttgcttacc atctggcgct aactgattgg ctatagttgg aactgctggc
      241 catacaaaca tttctccaaa agttaaaata atcataccga caacaaatat tgtaaagttt
      301 tcggcaaaac tcgtgacaaa gaacgacaac ataaaaatga tgatgccgac aaacatttgc
      361 ttctttaagt ttccttttaa cagatagaga accggtttaa ttaatggttg tgctactaaa
      421 atcattattc cgttaattgt ccataaaaca ctatattgtg ccattgaaat attaatagat
      481 tgtgtaaatg atgcgattgt agactcccat tgaatatacg caacccaaca aattgcaaac
      541 attgcacaaa ttagtactaa tgaaataaat cttgctttat tcttttttcc agtaatatct
      601 aaatgagttg gatatttaac ttttgcatta atttcaatat taaattgcgt taccgcgaca
      661 agcgcaaaca caacatacat aataagattg gctaaaaaga tatagttaaa gctaaattct
      721 gcgacaaagc cgcccattgc agcaccgaca gccacaccaa tattttgcgc taagtatatc
      781 gcattaaacg tttgtcttcc gccattcggc cacactgctc cagccatagc gtatatcgca
      841 ggaataatca ttccgccacc aaagcctaac attacaagcc atacagcata ccaaggccac
      901 ccgtgaaaga aattaagtag cgttgtacta caaagacaag tgaaagttcc aattaaaatc
      961 gtcttgtatc cacctaattt atcaaatagt gaaccaccta ataagtttcc aataaccatg
     1021 ccaaatgaat ttatcattag cactaaacca gcaacagtta aactttttcc aagttcttgt
     1081 ttcatataaa ttgtatttaa aggccacaaa aaactggaac cagtaatatt taacgccatg
     1141 ccaattacta gccaccagac tgatttaggt atattcatat ttcatagttc ctctctcttt
     1201 tttgtcaaac tataataaac actataacat taaaatatgt taaaatctgt acttagaact
     1261 ctttttttat agaaaggaaa atgacatatg ggcaatcatt tccaatacgc ttttgaaaac
     1321 aaacgttatc acacatggaa ttaccattta aaaaataaat ttggacaaaa aatatttaaa
     1381 gttgcattgg atggcgggtt tgactgtcct aaccgcgatg gcactgtagc acatggtgga
     1441 tgtacatttt gttctgctgc aggtagcggg gactttgcag gtaatcgtgc agattcaatc
     1501 gcagtacaat ttaaagaaat taaggaaaag atgcatgaga aatggcacga aggaaaatat
     1561 attgcttatt ttcaggcatt tacaaataca catgcaccag ttgaagtatt aaaagaaaaa
     1621 ttcgaacctg tacttaaaga accgggtgtt gtaggattat ctattggtac gcgtcctgac
     1681 tgtctaccag acgatgttgt cgaatattta gcagatttga atcaacgaac atacttatgg
     1741 gttgaattag gactacaaac aatccatcag tcaacatctg atttaatcaa tcgtgcccat
     1801 gatatgaaaa cttattatga tggtgtggca aaattacgta agcataatat caatgtatgt
     1861 acacacatca ttaatggctt acctggcgaa gactatgaca tgatgatggc tactgccaaa
     1921 gaagttgcac aaatggatgt acaaggtatt aaaattcatt tacttcattt gttaaaaggt
     1981 acaccgatgg taaaacaata cgataaaggt ttattaactt ttatgactca agaagagtac
     2041 acaaacctag ttgtggacca attagaagtg attccccctg aaatgatcgt tcaccgaatt
     2101 accggtgatg gtccaataga tatcatggta ggtccaatgt ggagtgttaa taaatgggaa
     2161 gtattaaatg gcatcgatgc tgaattagca cgtagaaatt cttatcaagg cttgcgttac
     2221 aagtctaagg tgaagcaatg aaattagaac gtatactccc tttttcaaaa acacttatta
     2281 aacaacatat aacaccagaa agtattgttg tggacgcaac ttgcggtaac ggcaatgaca
     2341 ctttattttt agccgaacaa gtaccagaag gacatgttta tggtttcgac attcaagatt
     2401 tagctttgga aaatacacgt gataaagtta aggatttcaa tcatgtttct ttaataaaag
     2461 atggacatga aaatattgaa catcatataa atgatgcaca taaaggtcat attgatgcag
     2521 ccatctttaa cctaggttat ttgcctaaag gtgataaatc tatcgtgaca aagcctgaca
     2581 cgacaatcca agctattaat tcattgctat cattaatgtc aattgaaggt attattgtac
     2641 ttgttatata tcatggtcat agcgaaggac aaattgagaa gcatgcattg cttgattact
     2701 tgagtacttt ggatcaaaag catgcgcaag ttttgcaata tcaattttta aaccaacgta
     2761 atcatgctcc attcatttgt gccatagaaa aaatttctta agcatttgct gcgctatact
     2821 atttataatt taggattgag tagtcaacat ttgaaaaatt aataccaaca acaacaaaaa
     2881 gaggttttca acttaatgaa aacctctttt tatgctatta aacttacaca gcaataattg
     2941 cgtttaaact attttgcatt gctgttgctc tacttgcaat ggcatcactg atgaaattca
     3001 acaactctac tttctcttgt tgtaactttt cattgaaatg aataataact gttctttcat
     3061 cgtcaacagg acgctctttg taaatccatt ctaattcaac taaattatta tacgttctcg
     3121 tacgcttata cggtttaact tcaacaaatc tgtccatttc tttaagcgtc atagaacctt
     3181 tttgccataa agttagtaaa attaaaattt cttctctaga cattttgtat tcgctttcaa
     3241 tctcgctgaa aattgagtta atgtcaccca aaagtgtttc taattgcaaa atcccaaata
     3301 cagtgtcgtt atttactttt ttcatgctaa acatctccca attaataatc acatatttag
     3361 ctaccataca agtattaaca aacatcccaa caatcccaaa acttgtatgt gctaacttat
     3421 gcataaaact acaagtgtaa ataaacttgc tttctattca attgtcaaag ttgaataaaa
     3481 ttaaataagt ataaaaacca ataatcaata gagttaatat ttagaaataa gataatagta
     3541 cataaaacaa atcactctat tacatattaa ttttataatt tatatacaaa gaaaatatat
     3601 caatattacc attaaattgc aacaattcta catatatttc ttcaaaaaag atacgcattg
     3661 aatttttatt acatataact ctcatgaaaa ttgattaaac taaattaatt agttagcctt
     3721 aaatttaaca aattaaatca aaccgaatga ggctaaattg gttatttttc aagatatgat
     3781 ttgtataaat caaaacattt cttacaaatc aaatgtgtat aaaatgtgat atacattctc
     3841 aaccttaaaa aagtaaaaaa aaagatgagc atctactgtc tcatcttttt agaatattaa
     3901 atttctacaa tttcgtcatc ttcaacaata aagcccattg tattgacgct gttatttaag
     3961 aaagtcagaa tataacgcat tacttcatca cgttctggct cattgtgaac ctcgtggtaa
     4021 aaaccttgcc aagctttaaa atataattca ggtgtttgat atttttcttt aaactcatca
     4081 attgccctag tatcaacaat taaatccttc gttccataca ttaatagcgt tggcattggt
     4141 tgaatgtcat gaatatgagc catcgtatct ttcatcgtct cattaattgt attataccaa
     4201 tgatacgttg ctttttttaa cattaaacca tcgttaactg tttcttcaac aatttctaaa
     4261 ttacgtgtta aatcttttgg ttctacacca acattaaaac gtgtgtcttt tgaaatttta
     4321 cctatatttg aaacaagttt atctttacga ttttttccat tcttttgaag ttctagcata
     4381 ggagaaatta acatcatccc ctcgattggc aattctactt tttcaagtaa atttaataaa
     4441 atcaaaccgc caagtcctac ccctaataca taagtaggaa ttttatattc attagctatc
     4501 tttaaccagt ctagcaaact ttcgtgatac gtttgaaagt tttcaatttg tcctttatta
     4561 gctcttgaag tttgaccttg accaggcaaa tctcccataa tcacatgata gccatttctt
     4621 cttaacatcg taataacata tgcatatctt cccgtatgtt ctaatatatt atgagcaata
     4681 acaacgacgc ctttcgcatc attttcagct tcccacttcc acattattat actgcccctt
     4741 tttcattaat cttcaataac ataattatag caaattcact atgtagattt ctatttatag
     4801 tattattgtt gtccatatta ttatatataa atgaaatcaa catcaataat agtgtaatta
     4861 tacataatta tttttgattg tttttgatga aaacgctttc tcgaatattt ttttcatgct
     4921 aaacttattg taaacacaag ggtttggagg agtagcaatg gcactattaa agaatttttt
     4981 tatcggatta tctaataata gttttttaaa caacgcagca aaaaaagtgg gcccacgttt
     5041 gggcgccaat aaagtcgttg ccggaaatac aattccagag ttaattaata caatcgaata
     5101 cttaaatgac aagaatatcg ctgttacggt agacaattta ggggaatttg tcggtacagt
     5161 tgaagaaagt aatcatgcta aagaacaaat tttaacaatt atggacgcgc ttcatcaaca
     5221 tggcgtaaag gcacatatgt ctgttaaatt gagtcagtta ggtgcagaat tcgacttaga
     5281 attagcttac caaaatttaa gagagatttt acttaaagca aatacttaca acaatatgca
     5341 tataaatatt gatactgaaa aatatgctag cctgcaacaa attgttcaag ttttagatcg
     5401 attaaaaggc gaatttagaa atgttggtac tgtaattcaa gcatatttat acgatagcca
     5461 tgaattagtt gataagtacc aagatttacg attacgtttg gttaaaggtg catataaaga
     5521 aaacgaatca attgcatttc aatctaagga agacgtagat gcaaattaca tcaagataat
     5581 tgaacaacgt ttgttaaacg cacgcaattt cacttcaatt gcaacacatg accatcgtat
     5641 cattaatcat gtaaaacaat ttatgaaaga aaatcacatt gaaaaagatc gtatggaatt
     5701 ccaaatgctc tatggtttta gatcagagtt agcagaagaa atcgcaaatg aaggctataa
     5761 tttcactatt tatgtacctt atggcgatga ttggtttgcg tattttatga gaagattagc
     5821 agaacgccca caaaacctat ctcttgctgt aaaagaattt gtgaaacctg ctggcttaaa
     5881 acgtgttggc ataattgcag ctttaggagc tacagttatg ttaggtttaa gtacaattaa
     5941 aaaattatgc cgtaaataga gcaagacata aacaataatt taggagtctg gaacaataat
     6001 caatgttcta ggctcctaaa tgttatattg gcagttctga atcatttata aatctttgat
     6061 tatgaactca caacagtgct ctatgctttt atagatttta ataaattagc catttcaatt
     6121 gcacttactg ctgcttcagc acctttattg ccagctttcg tacctgctct ttccacagct
     6181 tgttcaatac tttcagtcgt taaaatacca aatatgactg gtacattagt ttgatcattt
     6241 actttagaaa cacctttcgc gacttcatta caaacataat cataatgaga cgtagcaccg
     6301 cgaattacgc atcctaatgt aattactgca tcataatttc ctgatgaggc taatttttta
     6361 gctactaaag gaatttcaaa cgcacctggc acaaatgcta catcaatatt gtcttcatta
     6421 acatcatgtc gaatcaaagt atcttttgca ccttcaagta atcttccagt gataaaatca
     6481 ttaaatcgac taactacgat tgcaactttc aaatcttttc caattaattt accttcaaaa
     6541 ttcatgttaa aatcctccta tattaaatga cccattttta tttttttcgt ttccatataa
     6601 tcatgattat gtaccgtttc tggtacgata acttcaattc tttctgcaat atcaatgcca
     6661 tattgtttta atccctcaaa tttacttgga ttattactta ataaattgat atgttcgatg
     6721 ttaaaatatt ttaaaatctg tgcagcaata tgataatctc gcaaatcttc atcaaaacct
     6781 aatgctaaat ttgcagttac tgtatcatat ccttgctcaa ttaattcata tgcgcgtaat
     6841 ttgtttaaca atcctatgcc acgaccttct tgaggtagat aaataatcat gccaccatgt
     6901 tcattgatat acttcataga cgattcaagt tgagcaccac aatcacaacg ttgactatgg
     6961 aaaatatcgc ctgtaaggca cgcagaatgt aagcgtacat tttcatgttg tcgaattgca
     7021 ccttttgtca gtacaactat ctcttcatct gtgtatgtcg ctttaaaacc atacatatca
     7081 aatgttccga aatctgtagg cattttcact tttgccttaa attcaatttc tggttctaat
     7141 tttttacgat attcaattaa atcatcaatc gtaatcatct ttaattgatg tttttcttta
     7201 aacttttgta aatcttgtcc tttcgccatc gtgccgtcat cattcataat ctcacaaatg
     7261 acaccagcgg gcttggcacc agtaagttta gctaaatcaa cagccgcttc tgtgtgtcca
     7321 tttctagcta atacgccttt atcttgtgct actaatggaa ataaatgacc aggacgatta
     7381 aaatctttag cttcactact aggatcaatg agctttttgg cagtcaatgt acgttcataa
     7441 gcactaattc ctgttgttgt atctacatga tcaatactca ctgtaaattg cgtaccaaag
     7501 atgtcggagt tatcatcaac catttgtacc aaatccaaac gttgtgcaat atctttagac
     7561 actggtgcgc atattaatcc ccttgcttct ttcgccataa aattaatggt attatcgttc
     7621 atccattcag tgaccgctac taaatcacct tcattttcac gattctcatc atctactaca
     7681 ataattgttt ctccattttt taaagccatt aaagcactgt caatattatc gaattgcatg
     7741 ctacccctcc taaaaaccaa atgctcttaa tttatctaca gataattggt ctttatcttt
     7801 atttaaaata ttttcaacat atttaaacaa aacgtctgtt tctaaatgta ctttatctcc
     7861 taattttttg gatgataaaa tcgttgaacg cctcgtttct ggaataagat gaatgtcaaa
     7921 actgttatca tgcttatcaa ataccgttag acttacacca tccacagtaa tagacccttg
     7981 cttaactaac tgattattaa tatgttggct acattgaatc gtaataattt tggcattggc
     8041 tgtttcattt atttttgaaa ctgttcctag ttcatctaca tgaccgagga caaaatgtcc
     8101 accaaaccta ccgttaccac ccatggcacg ctctaaattt acttctgatt gtcgcttaac
     8161 atctgctaaa taggttttat tttcagtgcc tttaattact tgaacagtaa aagatgtctg
     8221 attaaaatca atcactgtta aacatgcacc attaacactg atggaatcac caatatgcat
     8281 atctgccgta atcttatgtg cttcgatttc aatcgtcctg actgattgac gaatttgaac
     8341 acttttaacg acacctattt cttcaacgat gccagtaaac atgcatcatc acttctttcg
     8401 taaagttaat ttaacatttt gatttaataa ctcggaatga acaatttcaa attggttcgc
     8461 atctggtatc tcaatcacat catttgtttg ataaaattga taatttccag atccgccaat
     8521 taatttcggg gcataataga gaataaattc atctatataa ttagattgga gaaattctga
     8581 agtagtggtt ggacctgcct cgactagcaa agttccaact cctcttttat ataaattgtg
     8641 aagaattgtt gttaaatcgc aagacttcaa gtaaataatt tcaatatgtg tttgattggt
     8701 tgttaaattt ggattttcag tatatatcca aattggtgtt gattcatctt gataaatttg
     8761 ctgattaaaa tgaatattcc cagacttaga caatattact tttatagggt tttttccatc
     8821 ttgaatacgt gtagtatatt gtggatcatc taattcaact gtacgtcttc cagttaacac
     8881 tgcgtcgtgt cgatgtctta acttatagac atcttgttta acctctttgt tagtaatcca
     8941 ttgactttgt ccattatcat tcgcttgttt accatctaaa cttgcagata ctttcactgt
     9001 aatttgtggc agttgctttg cttttgcttt aaaaaaggct tggtataatt gtgatgcccg
     9061 ttcatcatca acgcattcaa cctcaatacc gtgagcccgt aacgtctcat caccatgtgt
     9121 gtctaacgaa ttgtcttttg ttgcgtatac tacttttgct atcttacaat caattatttt
     9181 gttaacacag ggtggtgttg aaccaaaatg actacatggc tctaacgtaa tataaatcgt
     9241 tgcaccttca gcattttgtt gtgccatatc aagtgcttga acctccgcat gcttgtcacc
     9301 ttttctcaag tgtgcaccaa taccaacaat cctaccttct ttaactacaa cagcgccaac
     9361 gggtggatta acacctgttt gaccttgtac catatttgca agttgaatcg cataatccat
     9421 aaattgactc aaatgatcac ctctataaac aaaaatcctc acatcatgaa ttaagatgca
     9481 aggagaaaaa tttatcgtta aataagccta tttgtacaca tttttacaaa tacgctacat
     9541 tatctttgtc gataattaac attctttctc ccatccagac tttaactgtc ggctctagaa
     9601 tctcactaga tcagccacta atatgaaaca tattagcagg tcgcaggctt tatttactgc
     9661 cggttgggaa tttcaccctg ccccgaaaga attatatatg aaattgttat agattatttg
     9721 agtacgtagt atgtcaacta catttaaaat gatactatat gttttctgaa aaaacaatta
     9781 atgacggttt taatttaata taatctgagt actataggca tctcattgat atgattctta
     9841 ctaacagaca ttaaaatcaa accttcaatt cgtctctata gagcgttctc tttattatct
     9901 tctagttaca aattattgat tgtcactgcg ctgttgttgc tcattcgatt ctaaagcatc
     9961 atataattga gatactgtat gcgcaacttg ttctactatc attttcacac catttcgtag
    10021 tttattaaca ccgtttgtca tttgacctat cgcaatcata tttgttaatg ttccaaacct
    10081 tggactaata acttgattgg tttccggtat gatttgtatg cctcccattg ggtgtgcttg
    10141 tacaatttgt ctattttcaa gatttttaat taattgatca tcttgatcca attcatttaa
    10201 atgacttttt gcacctgtcg cgttaatgac aacattatat atgtctactg attcttggtt
    10261 tttgtatgaa aaataataca acttgccatc atgtttcaca tcttctaaat cttttttcaa
    10321 aattaaagac ttattttcta ttaattcaat aattagttca gcagttcttg gaggcattgg
    10381 atttgaattt aattgaatca tctttgagta tttttgatta aattgatgtt ggtcttcaat
    10441 acttaagcta ttccatatcc aatttaaatt ctctttcaaa tgttcaatca tactttggaa
    10501 aatgcccatt tctgttggac gcgctaaatc atacttcaaa tctgcaatat gatttcctgt
    10561 acgtctatgt actaattttt taaaatcaat atcatattca gcacattctt ttaaaaataa
    10621 agaaactaaa gtatcaagcg gtgcattgcc gaaatgatgt tttttaatgt catttaattt
    10681 gtctttagtt aagtacttga atgtcacgtc tatcattgta cctcttacac ttggtaaatg
    10741 agcagaacga ctcgtcatag taattggtaa tttcggatga tgagcagcaa cataacggac
    10801 aacatctaaa ctggcaaggc ctgtaccaat aatcgcaata tcgtccagtt catttacttc
    10861 gtctaacgta ttatatgttg gataaggcgt agcgatatat ccttttttac cctttaagtt
    10921 atatggatca tggtaggcaa atgtaccaca tgttaaaaat acataatcgt acgcttgcca
    10981 tgattgttct gaatttgtag tacatatgta ataagttaaa ttcgtttcat cgatattaga
    11041 atttgtataa atctcttgaa ctttattata attagttgat atatttggat attttttcgt
    11101 gaacatagat aaataagatt tcatataatg tccgaataca aatctaggta aatatgcagg
    11161 ttcatcaaaa ttaaaatcag tttgttgttt ataccacttc caaaattcag tctcatcatc
    11221 taaatttaaa ctcatctttt tcgaaggcat attaattagc agctcagaac tatcattttg
    11281 aaatggtacg ccctgtccca tatttacttt atcatcgtat aaatctatat ctaattgatt
    11341 aaacttcggg tgcttaacta actctctcaa tacacttaca ccagcagttc ccatgcctat
    11401 tattgctaca cgcatatatc atccatcctt ttgtaattca aaatattata tttaaactat
    11461 tacctataat tatagcaatt tggtattttt aataaaattt attaaggcaa gctattattt
    11521 cactttcaca accattaaac catttaaaac ttttcattta atatcaacct tagtgtttgt
    11581 gttaaaatat ttttaacgag gtgaattaaa ttgaaattta tagcaaagat attaatctca
    11641 ttagtaattc tatttatgat ttttagtcgt aaaaacaaag cttcttaatc aatacccttg
    11701 attacaaaat aaattcaact aaggtgattg gccatacact caagatatgg ctaatcacct
    11761 ttttcaatgc aaaacaaaga gtatattttg caaaatcaat atctataatt tatctttaat
    11821 acccttttaa tactaaaacc aaatttaaat tacatagtca tatcgtgtaa tataccaaag
    11881 gaacatagac attcatctat atagatgata atctatttaa cgtctaggag gtatgttatg
    11941 acatataaag aactagcaac atttttaaaa gttttatcag attcaagcag attagaaata
    12001 ctagatttac tttcttgtgg agagttatgc gcttgtgatt tgttagcaca ttttcaattc
    12061 tctcaaccta cacttagcta tcatatgaaa gcattagtaa aatccaactt agttacgaca
    12121 cgaaaaatcg gaaataaaca tttataccag ctcaatcata atatttttga gtccgtaatt
    12181 aataacttgt ctaaggttca tacctctaac caacgatgta tttgtcataa ccttaagact
    12241 ggtgaatgct aatgatgaca actttagcga cactcatttt tctagtaact ttattatttg
    12301 tattatggca acctaaaggc ctagatattg gcattaccgc attaactggt gcctttattg
    12361 ctgttattac tggtgttgta agtttttccg atgttttcga agtaacaggt attgtttgga
    12421 atgctacttt gacttttgtc tcagtcattc ttatttcatt aatattagat aaagttggat
    12481 tattcgaatg gtcagctatt cacatgcttc atgcttcaaa aggcaatggt ttaaaaatgt
    12541 tcgtttatat catattattg ggtgccattg ttgctgcatt tttcgcaaat gatggcgcag
    12601 cgttaatctt aacgcctatt gtattagcga tggttaaaaa tataggtttt agtaagcggg
    12661 ccatattccc ttttattatt gcgagtggtt ttatagctga cacaacttct ttacctttaa
    12721 tcgtgagcaa tctagtgaat attatatctg ctgattattt tcatgtagga ttcgttcgat
    12781 attttagtag aatgattata cctaatttat tctcgctttt agcaagtatt atagtattgt
    12841 ggttatattt tagaaaggcg atacctaaaa cgtttgatga taataatata aagcatccta
    12901 aagatgccat taatgattta aagctattta aaatttcatg gattgttcta gttatattac
    12961 ttttcggcta tctaatcagt gaatttacta aaattccggt atcaattttc actggaatca
    13021 ttgcttttat ttttctaatg ttggctcgta aatcaaatgc tgtaaatatt aagcaagtca
    13081 ttaagggcgc accttggaat atagtattat tttcaattgg tatgtatatc gtcgtattcg
    13141 gcttaagaaa tgctggcatt actttaatat tggctaaaat attagaatat atttccaatt
    13201 acggtctatt tagcactatt ttgggaatgg gcttcatttc agcgttttta tcatcaataa
    13261 tgaataatat gcctacagtt ttaatagatg cgattgctat tggtcaatca aatgtccatg
    13321 gcatgttaaa agaaggccta atttatgcga atgttatcgg ttctgattta ggtccaaaaa
    13381 ttacaccgat aggctcttta gctacattac tgtggttaca cgtcttaaca caaaaagatg
    13441 ttaagatttc ttggggcaca tactttaaaa ctggtatcat cattacaatt ccagtactat
    13501 ttataaccct cttaggattg tatctaacac ttatcatatt ttaagaaata aaaagacgct
    13561 ttctatttat tgcgatagaa agcgtctttt tatacttgag caagtcatat cataacttaa
    13621 ctctatcaaa ttacttattc aaatgtttac tgtcatcttt atacacaaag tatttgaagt
    13681 attttccttc agtcttcata ttcttataaa agtcagcgat aattgttgca ttactttctg
    13741 cccacttaat atctgtagca tattgatgtt ctcctggatt ttttggattc catctcatac
    13801 tatacaatgt attttgatct gtgcttgata agaagtgctt atgaatgaaa tcagcaccgc
    13861 ctgaaatagc tttttcaggt gtatcccaac catgcttttt agcatattct gcacctgttt
    13921 taattgggtc tttatcaagg gctcctactc catagaaatt gtagtatttt ttcccatcaa
    13981 tttcgactcc attagctaat tcacttttaa ctgcgccagt ttctaataat gcatgtgaaa
    14041 ttaaataaac ttcgttaacg tgcttatctt tagcagcttt taagaaatca tccgtatgtt
    14101 tcaataacgt tggtctatct actaacatac gtttaattct atttttatca atcccttgat
    14161 actttgataa atctaaaaat tgatattttt gcttttcatt atcgataaaa gtaccgctat
    14221 ccattgcact tttaatttca gttgcagatg catctctcca tgcatcattc tttttatttg
    14281 atacctgttg actcgtataa ttatttattt gtttctttgc tgcatcgttt aatgtaacat
    14341 ttaacttttc aatcttaatg tcggatttaa catgtttgaa aaatatctga tcggatatca
    14401 ttgagaaaaa taaaaatgag acaacagcaa atatgacaac aagtcctatt attccaaaaa
    14461 tagaaccttt cttgtgttta ttcatatcca cacctcttag gtcattgttg ttatacattc
    14521 agtttgacta cgataataaa gtttaacatt attaaaaccg tttaacaatt ttatctatgc
    14581 attgttacat attcataaat aaacagtaat gtaaatgaaa cgttttaaac catttatcaa
    14641 cattacttta cgttgatcta cgaatcataa atgttttatc tttttgtata acctttgaaa
    14701 acctttatat caataatctt attattcacg tattaaaaaa caaaaaattc ttttgttcgt
    14761 ttacttagtt tcttttttat aatccatcat aataaaactt gctgcatttg atctattttt
    14821 ataatagtac tgatttaaat caatcgtacc ttcaatttga ccatcacgat acatcatttc
    14881 attagtgaag ttatttatca tcacgaaatc agcgcgatct cttataacat ctaaatcgtc
    14941 gtttcgtgca aaaaaatgtt ctaaatttaa atgtgcgtaa tccattgtta cctcccgatt
    15001 acaaattttg ttctatctac tatttagcaa agtaataata catcatatca cattaaatta
    15061 attacttatg tcttatattt aacacttaat ttagcgatta cgcaaatgaa tgatttttct
    15121 aaatatttta ttctaacttt atccggaaat atttgttata cgtataactt ggagaatgac
    15181 tacttgaaat ttaatgacgt atacaacaaa caccacaaaa tcatacacca tcttttaaaa
    15241 aaatataata ttagttataa ttatgatgag tattatcaac tactcttgat aaaaatgtgg
    15301 caattgagtc agatatataa accctcaagc aagcaatctt tatcctcttt tttattcact
    15361 cgattaaatt tttaccttat cgatttattc agacaacaaa atcaattaaa agatgtcatt
    15421 ttatgtgaga ataattcacc aacattaact gaacaaccaa catactttaa tgaacatgac
    15481 cttcgtttac aagatatctt caagctttta aatcaaagag aaagactatg gctcaaacta
    15541 taccttaaag gatacaagca atttgaaatt gctgaaatca tgtcattatc gctttcaacg
    15601 attaaattaa ttaagatgtc cgttaagcgt aaatgccaac ataattttaa ttagtttatt
    15661 gtgaaatttc ggtcgatgtg ttaatgatgt tgaaacaccc taacatcttc atataaattt
    15721 attttaacca ttaaattaac aacttattaa acgaggagta ctatatttgc aagacaattc
    15781 tactaaatat ctactttata tccaaactgc tacttcaaac catctcgaaa caaattgtgt
    15841 ctttttacat tgcgattata ttcttaaagt tccaattaac aaactcgttt catattatgc
    15901 gaaattgcat ttgtcatcac aaagtgtgct aattgagact gcaaaaaaca tactaaatat
    15961 taataaactg gttcctattt acatcaaccc aaaaactata ctttttccgt taaaacataa
    16021 acgtgcaccg atacaaattt atatcaatgc acattatatt gttggtatga ctgccataga
    16081 gaattcaaca ttaatacatt ttcaagaagg cattcagcta gaagttgatg agccattttc
    16141 tctagtttca aaaaaatgtc atgaaagttt agctttgaag cacttcattg aaaatactat
    16201 ttcgaattaa atttaatttc taaaagtttc ttagtaaagt atgatgtcac aacttttata
    16261 aaatatatgc taacaatcat taataaaatc gctccaataa ttgttaaaat aacaacagtt
    16321 gtagataaat ctaccttagg actaacgaaa aattgtacgc cagatagact tgtaattagc
    16381 ccaaataaaa acaagcctaa taaagcaaca taagcaaaca tcatcactac taatgtaaca
    16441 aacatactat aactttttgt ttctttattt cgaaatacat gttgtcttaa gtaagcatta
    16501 gcaaaggtaa caggcttatt taagttaacg ctgtcatcat cagatataga agacaatcgt
    16561 tgatctaatg cttgtttctc cttcttgttc aaaaaccata attgcttttt aactttttta
    16621 tcaaatgatg ctttattcat aattaaggat ttctctcctt tataatattg acgtaatttt
    16681 aaagattagc gtcaattgaa agtgtatttt atatatttta cagaaatata tgtatcaaat
    16741 ctacaaaaat ccatattaca attttattta tggtgatttg acgctaaaat acagtctgct
    16801 aattttttca aactaatgct ttttaggtaa gtacaatgat aaattttaat cgtataaatg
    16861 tttcatatat aatgaatcta gccttaacca tattgcattc aaaattaatt ttaaaaaatg
    16921 aaaagaaagg atttagcatg caaaaattca aagacttttt ttacgatgat ttatcggtta
    16981 cacgaggaaa ttatttttta actttaatgg cagcattttt tattactatc attttattta
    17041 tcggcatagg tgtcagtgaa gtacatttac tttatagtat gctgattgta ttagtaggtt
    17101 taattctatt gaggctattc aaaatcaatt tattctcttt taaaaaatta acattgtctc
    17161 aagttattta tattataggc ggtgcactat taatttatgg gttagataat ctttatttat
    17221 attttcatga cgtaccggca aatgaacaac aattagagca agaaatacga aatacaccat
    17281 tctatatttc tattttcact gttaccatca tccccgctat tgtggaagaa attgtttttc
    17341 gcggtatgat aataagggtt atcttcagaa aacacttgtt tttagggtta attgtgtcta
    17401 gtttagtttt tgcatcatta cacgaatctg acacttggat tggttattta ccttacttat
    17461 attctggttt gatttttggt ctaatttata taaaaacaaa acgattagaa gtggtaatat
    17521 tcatgcactt cttaaataac ttgttagctc tgctctttat aatatggaga taattttgag
    17581 ataacctttc atattatttt caaattatct atctgatatt tattttattg aacttgagtc
    17641 accacttaaa ctcaaaaata cgtgtcatca agtgcaaaat aatttagaac taatacaaac
    17701 ataaaaactt ttgctttacg cttaaaatca aaagtcacat acctatatat aagtacaact
    17761 tgtatatttt gtactctaat aggtatgtgg cttaaataat atttaattct tgagttgcat
    17821 atagtaattg tttttattca ttttctaaaa tatgcgagac taatttcctt ttctactcct
    17881 gttgaatcaa tttagacttt tttataactc gagcttttac ttagctgaat tttgacttct
    17941 tcttcattaa taactatgat ggaaaaaccc ccataagcac aatgcctatg agggtttaaa
    18001 tatgtttata aattatagaa tagaaagacc tgaagattga atatctttcg caaagccttt
    18061 aactgtatct actgataatt cgttaatatc gcgacctaag tttgtattca cttttttcac
    18121 aacatctgca gggcatgtaa taatatctgc accaatttca tcagcttgaa tcacattgaa
    18181 taattcgcgg caacttgccc ataataattt aacgccgtct ttactatgcg taacttttac
    18241 agcctctttc attaatggta atggatctac gcctgtatct gcaatacgtc ctgcaaatac
    18301 tgaaacatat gttggcacac cttcagttac tgcttcagtt atttctttaa cttgttcaat
    18361 cgtgtaaaca gccgtaacgt ttaatctcac attgtcagct gaaagctttt taattaaagg
    18421 aatcgttgat tcaccttttg tatttacaat aggaatttta acaaatacat tttcgccata
    18481 ttgttttaga attgctgctt ctttttccat agtttctaaa tcgtctgcaa atacttcaaa
    18541 tgaaattgaa gcatctggaa tttctttcac agcttcttca gcaaaagctt tgtaatctgt
    18601 tacgcccgct ttcgccatta aactaggatt tgttgtaaaa ccatccactt gtttgttttt
    18661 ataagctgct ttcatttctt caatatctgc accgtccgca aatacttcta catttagttt
    18721 agccatataa tatagcctcc ttgattctta ttaaaatttt aacaacatct gcatgtcttt
    18781 ttcttacaac catttgtaaa aaatgatttt tatttc
//
LOCUS       DAHHYJ010000040        18590 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000040 DAHHYJ010000000
VERSION     DAHHYJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.040"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            59..1405
                     /locus_tag="NBK22_001045"
     CDS             59..1405
                     /locus_tag="NBK22_001045"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCD2414241.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMAIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            1427..2242
                     /gene="ccsA"
                     /locus_tag="NBK22_001046"
     CDS             1427..2242
                     /gene="ccsA"
                     /locus_tag="NBK22_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCD2414242.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNSRYLIYYNIILFCLNMINLFFATHFVN"
     gene            2284..3210
                     /gene="hemC"
                     /locus_tag="NBK22_001047"
     CDS             2284..3210
                     /gene="hemC"
                     /locus_tag="NBK22_001047"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCD2414243.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQNEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            3232..3900
                     /locus_tag="NBK22_001048"
     CDS             3232..3900
                     /locus_tag="NBK22_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCD2414244.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQHYDW
                     LIFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKDNEVVKIDLYTSVPNKQNIQDVKEMIEHQEIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            3903..4877
                     /gene="hemB"
                     /locus_tag="NBK22_001049"
     CDS             3903..4877
                     /gene="hemB"
                     /locus_tag="NBK22_001049"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCD2414245.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAVAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            4925..6211
                     /gene="hemL"
                     /locus_tag="NBK22_001050"
     CDS             4925..6211
                     /gene="hemL"
                     /locus_tag="NBK22_001050"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCD2414246.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRNITTEYGALLIFDEVMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            6272..6373
                     /locus_tag="NBK22_001051"
     CDS             6272..6373
                     /locus_tag="NBK22_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414247.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            6407..7480
                     /locus_tag="NBK22_001052"
     CDS             6407..7480
                     /locus_tag="NBK22_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD2414248.1"
                     /translation="MTMIYRNNFIVFVLSFFISILLYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILLIAIVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTGLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYVIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(7703..8263)
                     /locus_tag="NBK22_001053"
     CDS             complement(7703..8263)
                     /locus_tag="NBK22_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCD2414249.1"
                     /translation="MNECAFGTKDPVYLNYHDHVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPKHN"
     misc_binding    8321..8622
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8666..11296
                     /locus_tag="NBK22_001054"
     CDS             8666..11296
                     /locus_tag="NBK22_001054"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCD2414250.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVKDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNSNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            11309..12580
                     /locus_tag="NBK22_001055"
     CDS             11309..12580
                     /locus_tag="NBK22_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCD2414251.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            12850..13557
                     /locus_tag="NBK22_001056"
     CDS             12850..13557
                     /locus_tag="NBK22_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCD2414252.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFALIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            13554..14240
                     /gene="radC"
                     /locus_tag="NBK22_001057"
     CDS             13554..14240
                     /gene="radC"
                     /locus_tag="NBK22_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCD2414253.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIKVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPCEIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            14308..14592
                     /locus_tag="NBK22_001058"
     CDS             14308..14592
                     /locus_tag="NBK22_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414254.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(14709..14906)
                     /locus_tag="NBK22_001059"
     CDS             complement(14709..14906)
                     /locus_tag="NBK22_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414255.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            15066..15539
                     /locus_tag="NBK22_001060"
     CDS             15066..15539
                     /locus_tag="NBK22_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCD2414256.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            15613..15732
                     /locus_tag="NBK22_001061"
     CDS             15613..15732
                     /locus_tag="NBK22_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414257.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            15931..16773
                     /gene="mreC"
                     /locus_tag="NBK22_001062"
     CDS             15931..16773
                     /gene="mreC"
                     /locus_tag="NBK22_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCD2414258.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSQGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSNLYIGEVTKVQNDQYGLAKEVRVKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            16773..17303
                     /gene="mreD"
                     /locus_tag="NBK22_001063"
     CDS             16773..17303
                     /gene="mreD"
                     /locus_tag="NBK22_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCD2414259.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMHIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKNFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    17330..17526
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            17539..17847
                     /gene="rplU"
                     /locus_tag="NBK22_001064"
     CDS             17539..17847
                     /gene="rplU"
                     /locus_tag="NBK22_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCD2414260.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            17853..18173
                     /locus_tag="NBK22_001065"
     CDS             17853..18173
                     /locus_tag="NBK22_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCD2414261.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            18185..18469
                     /gene="rpmA"
                     /locus_tag="NBK22_001066"
     CDS             18185..18469
                     /gene="rpmA"
                     /locus_tag="NBK22_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCD2414262.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
BASE COUNT         6481 a         2524 c         3503 g         6082 t
ORIGIN      
        1 ttggatttta aattatgtta taatatttgt attgttagta tatatggggg cgtttcaaat
       61 gcattttatt gcaattagta taaatcatcg cacagctgat gttgcactaa gagagcaagt
      121 tgcttttaga gatgatgcct tacgaattgc ccatgaagat ttatatgaaa ctaaatctat
      181 tttagaaaat gtcatattat caacatgtaa tcgaactgaa gtatatgctg ttgttgatca
      241 aattcacaca ggtcgttact atattcaacg atttctagct cgtgcatttg gatttgaagt
      301 agatgatatt aaagcaatgt cagaagtaaa agtgggggac gaagcagtag aacatttatt
      361 gcgtgtcact tctggtttag attcaatcgt acttggagaa actcaaattt taggtcaaat
      421 aagagatgca tttttcttag cgcaaagcac aggtacgaca ggaacaattt ttaatcatct
      481 atttaaacag gcaattactt ttgcaaaaag agcacataat gaaacagata tagctgataa
      541 tgctgtaagt gtgtcttatg ctgcggtcga gttggcgaaa aaagtatttg gcaaattgaa
      601 aagtaagcaa gctatcatta ttggtgcagg ggaaatgagt gaattatcac tattaaatct
      661 tcttggttct ggaattactg atattacagt agtaaataga acaattgaaa atgctatgaa
      721 attagcagca aagcatcaag tgaaatatga tgaactatca tcattaccaa atttacttga
      781 aagtgcagat attgtgatta gttcaacgag tgcacaatct tatatcatta caaatgaaat
      841 gatagaaaga attgcagaaa atagaaagca agattcacta gtattgattg atattgcagt
      901 tcctcgagat attgaacctg gtattagtgc catcacaaac atctttaatt atgatgttga
      961 tgacttaaaa ggtttagttg atgcaaactt acgtgagcga caattagcgg ctgcaacaat
     1021 ttcggaacaa attcctgcag aaatacatgc acacaatgag tggattagta tgttgggtgt
     1081 tgttccagtg attagagctt tacgtgaaaa agctatggca attcaagcag aaacgatgga
     1141 cagtattgat cgtaaattgc cagggttaag cgaaagagaa cgtaaaatta tatctaaaca
     1201 tacaaaaagt atcatcaatc aaatgttgaa agatcctatt aaacaggcca aagaattaag
     1261 tagtgataag aaaagtaatg aaaaattaga gctatttcaa aatatatttg atatcgaagc
     1321 tgaatgtcct catgagcaag cgaagcaaca aaaagaaagc aaggtcaagg aaatttcagc
     1381 gcgacgtatc tttagttttg aataagcata ttaatatggt gataatatgc aagaaaacct
     1441 gtttattcga ttcaatgaaa ttatattatt aatatactta atcagtatca tttgctattt
     1501 ttatgatttt gtacaaaaaa gtcataagat tagaagttta ggcatatatt tattggggat
     1561 tgtttgggtt ttacaaacaa tctctttatc tatttttatt atacaaacta gacatattcc
     1621 attagggtct atttcagatg tattctatac tttaagttgg ctgattattt caatctcttt
     1681 aattcttaac cttatcaaag ttttgaattt ctctgtgttt ttcttgaatt taatagggtt
     1741 aacactttta gggatgaata catttcaacc gacacattat actaataagg ttcagaaaat
     1801 tgctgttgta gacgagttac tgttagtcca tatcggtcta gctgtgttaa gttatgtctt
     1861 ctttgcctta gcttttgtca atgcattact gtatatcatg caataccgta atttgaagga
     1921 aaaacgtttt gatcaaaaat actttagaat tggtagcgtt gctacacttg aatcaattgt
     1981 gttttactca acgttaagtg ggtggataat tctcatattt agtattattt taggtacaca
     2041 gtggggtgta atttcagttg gtgaacgtat attcattgac ccgaaggtaa tactctcttc
     2101 aattattaca gtattatatg gaagttatat tttacttaga atcaacaaat ggttgaattc
     2161 aagatattta atatactata acattatttt attttgctta aatatgatta atttattttt
     2221 tgcaacacat tttgtgaatt gagaaaataa tctagagcaa tccgttccta ggaggaattg
     2281 aacatgcgta aattagtcgt tggctccaga agaagtaaat tagctttaac acaaagccag
     2341 caatttattg ataaattaaa agctgtcgag ccaaatctag aaattgaaat taaagaaatt
     2401 gtcacgaaag gcgatcgtat agtagataaa caattgtcta aagtcggagg caaaggctta
     2461 tttgttaaag aaatacaaca tgaacttttt gaaaaaaata tcgatatggc aatacactcg
     2521 cttaaagacg taccaagtgt aattccggaa ggtttaacat taggttgtat ccctgataga
     2581 gaattacctt ttgatgcgta tatttctaaa acacatacac cactatccca attgccagaa
     2641 ggcagtatta ttggtactag ttcattacgt cgtggtgcac aaatattatc taagtatcct
     2701 aatttagaga ttaaatggat tagaggtaat atagatacac gattagaaaa gttacaaact
     2761 gaagattatg atgcgattat tttagctgca gctggtttaa gaagaatggg ctggtcagat
     2821 gatattgtaa catcttatct tgatagagat acattgttac ctgcaatcgg acaaggtgct
     2881 ttagggatag aatgtcgtag tgacgatgaa gaactattaa cattattaag caaagtacat
     2941 aatgatgagg ttgcaaaatg tgtgactgct gaacgaacgt ttttagcaga aatggatggt
     3001 agttgtcagg tgccaattgc aggatatgct acaatctcag atcaaaacga aatcgaattt
     3061 acaggtttaa ttatgacccc agatggtaaa gagcgatttg aatatacaat gaacggaaca
     3121 gatccggttg agttaggcaa aacagtgagt aacaaattaa aagagcaagg tgcttatgaa
     3181 attataaaac gcttaaatga acaacattaa ataatggagt gacgtttaaa tatgaagcca
     3241 gttgtagtta tgacacaaac aaatgacatg caaagtgatt tagtatcaat tattcacaaa
     3301 ccctttattg atataaagcc actaaatttt gatattcatt tgttaaatca acactatgac
     3361 tggcttattt tttcatctaa aaatgctgtg aaattctttt ataaatatct aaaaggaatt
     3421 aatgttgata acattgctgt gataggaagt aagacagcgc aatattgtga atcacttggc
     3481 attcgagttg attttatgcc aaacgacttt tctcaagaag gatttttaaa atcatttaat
     3541 caaactaacc aaaaaatact tttgccttcg agtgaattgg cgagaccatt gttattagca
     3601 gcgttatcta aagataatga agttgttaaa atagatttat atacttcagt gcctaacaaa
     3661 caaaatatac aagatgttaa agaaatgata gaacatcaag aaatcgatgc attaacattt
     3721 tcaagttcgt cggcagtacg ttattatttt aatgaaggat ttgtaccaaa attcaagtcg
     3781 tattttgcta ttggagaaca aacagcacgg accattaaat catatcaaca accagtaaca
     3841 attgcagaaa ttcaaacact cgaatcacta attgaaaaga ttttagaaag taggggctaa
     3901 aaatgaaatt tgatagacat agaagattga gatcatcagc gacaatgaga gatatggtta
     3961 gagagaatca tgtaagaaaa gaagatttaa tatatccaat ttttgtagtt gaaaaagacg
     4021 atgtgaaaaa agaaattaag tcattgccag gtgtatacca aatcagtttg aatttacttg
     4081 aaagtgaatt aaaagaagct tatgacttag gcatacgtgc cattatgttt ttcggtgttc
     4141 caaactcaaa agatgatata ggtactggtg catacattca cgatggtgtt attcaacagg
     4201 caacacgtat tgctaaaaaa atgtatgatg acttattaat tgttgcagac acttgtttat
     4261 gtgaatatac tgatcatggt cattgtggcg tgattgatga ccatacacat gacgttgaca
     4321 atgataaatc attgccactg cttgttaaaa cagcaatttc tcaagtggaa gctggtgctg
     4381 atattattgc gccaagtaat atgatggatg gttttgttgc tgaaattcgt cgtggattag
     4441 atgaagccgg ctattacaat attcctataa tgagttatgg tgtcaagtat gcatcaagtt
     4501 tctttggacc ttttagagat gcagcagatt cagcgccatc atttggggat agaaaaacgt
     4561 atcagatgga ccctgctaac cgtttggaag cacttcgtga attagaaagt gatcttaaag
     4621 aagggtgcga catgatgatt gttaaacctg ctctaagtta tttagacata gttcgagatg
     4681 ttaaaaatca tacgaatgtt ccagttgttg catataatgt gagtggagaa tatagtatga
     4741 ctaaagcagt ggcacaaaat ggttggatag atgaagaacg tgtcgttatg gaacaaatgg
     4801 tttcaatgaa acgtgcaggt gctgatatga ttattacgta ttttgcaaag gacatttgtc
     4861 gctatttaga taaataaggt tttatatgta tgattttcca taaactatag gaggaattta
     4921 ctttatgaga tatacgaaat cagaagaagc aatgaaggtt gctgaaactt taatgcctgg
     4981 tggtgtaaat agtccagtac gcgcatttaa atcagtagat acaccagcaa tttttatgga
     5041 tcacggtaaa ggttcaaaaa tttatgatat cgatggtaac gagtatatcg actatgtact
     5101 aagttggggg ccacttattt taggacatag agaccctcaa gttattagtc atttacatga
     5161 agcaattgat aaaggtacaa gttttggtgc atcaacatta cttgaaaata aattggcgca
     5221 gctcgttatt gaccgagtac cttcaataga aaaagtgcgt atggtgtcat ctggtacaga
     5281 agctacattg gatactttaa gattagcacg tggttatact ggaagaaata aaattgtgaa
     5341 atttgaaggt tgctatcatg gtcatagtga ttcgttatta atcaaagctg gttctggggt
     5401 ggcaacatta ggattgccgg attctcctgg tgtgcctgaa ggtattgcta aaaatacaat
     5461 tacagttcca tacaatgatt tagatgcact taaaatcgct ttcgaaaaat ttggagacga
     5521 tattgctggt gtaatcgtag aacctgttgc tggtaatatg ggtgtcgtac cgccgattga
     5581 aggtttttta cagggattaa gaaatattac gactgaatac ggcgcattgc taattttcga
     5641 tgaagtaatg actggtttca gagtcggtta tcattgtgca caaggttact ttggtgtgac
     5701 accagattta acttgcttag gaaaagttat cggtggagga ctacctgtag gtgcttttgg
     5761 tggtaaaaaa gaaatcatgg atcatatagc accattagga aatatttatc aagcgggtac
     5821 gttatcagga aatcctcttg caatgacaag tggttatgaa acgttaagcc aattaacgcc
     5881 agagacatat gagtatttta atatgttagg cgatatactt gaagacggtt taaagcgtgt
     5941 atttgctaaa cacaatgtac caataactgt aaatagagca ggttcaatga ttggttattt
     6001 cttaaatgaa ggacctgtaa ctaattttga acaagcgaat aaaagtgatt tgaaattatt
     6061 tgcagaaatg tatcgagaaa tggcaaaaga aggtgtgttt ttaccaccat ctcaatttga
     6121 aggtacattc ctatctacgg cacacacgaa agaagatatt gaaaaaacga ttcaagcatt
     6181 tgatacggct ttaagtcgta ttgtaaaata aatatacgga caaattgaga gcctgaactt
     6241 tgttcaggct ctttttaaat gtatatcagg catgggcggc gacttgatag tgaaagtcca
     6301 tatatgcttt gtagtcaaaa ctgctagcgg atattgttat cttaacaaac gtgaagctca
     6361 agcagcaatt tagtcatttt attttttatt gaaagaagtg gaaaacatga caatgatata
     6421 tagaaataat ttcattgtgt tcgttttatc attttttatt agtattctac tgtattcatc
     6481 gcacgtatta ctcccattta tgtttggtcc tattatcgca tcaatcattt gtgtaaaagt
     6541 tttcaaactt gatattaaat ggccattctt acttagtgaa ttaggtattg tactattagg
     6601 tgtgcaaatc ggatcaacgt ttacgaaaaa tgtcgttatg gatattaaag acaattggct
     6661 ttcgattatt gttgtatcta tttcgatatt attaattgca atagtaatgg catttatttt
     6721 taaaaaaatt gcacgtatta atacagaaac agctatttta agtgttatac caggagcact
     6781 aacacaaatg ctggtcatgg ctgaacaaga caaacgtgct aatttgttag ttgttagctt
     6841 aacgcaaaca tcacgaatta tatttgttgt tgttttagta ccgttcattt catatttttt
     6901 tcatgatggt aacatgcatg cgaatggaaa gttaacaaaa gtcttgcctt tatcacaagt
     6961 attaaacata gggcaaatag ttattttagc gatagctatc tttatagttt atctaattat
     7021 gtctaaaata aagtttccaa catttcaatt attagcacca ctcattgtat taattgtttg
     7081 gaatttttct acaggtttaa catttacact agatcattgg ttgttgaaca tggcacaact
     7141 aatatatatg attagaattg gagttcaaat tgcgcattta ttgtcagatt taaaaggtag
     7201 actagcaatc gcaattacaa ttcaaaatat tatgttgata attggtgcgc taatcatggt
     7261 ttatgtcata catttctttg ataataatcc tattaatgaa ttgtttcttg gtgcagcacc
     7321 aggaggggtt aatcagatag ttttagttgc attagctact ggtgccgatg ttgctatgat
     7381 tacgagttat catattttcc gaatcttttt catcctattt attattgcac caggaattaa
     7441 ttacttttta aaatatcgtt cgaataagcg tgaagattag aaactatcat ccatttaagt
     7501 tgatttatgc ttaggataga attcattatt aaggtttaag caaaggttgt acatctattt
     7561 ttagattaag atatctaata ttagcagtgc attaacttgg aactttaact tatgatttga
     7621 ataaattttc acatcaaaaa acgagacagt ctattagtta aaaatagatg atgtctcgtt
     7681 tactttatga tgctatagca aattaattgt gtttaggctt tgatggacaa tcttttaaat
     7741 gtgcatcata taaaccagct gcttctaaaa acgaaaatac tgttactgga cctaaaaatt
     7801 taaacccgta ttgttttaaa tctttagata gttgtgttgc agtatcatca actgtgatac
     7861 gatcagaagc atgttcatac tgcaaatctt taggcttacc atttacataa gaccataaaa
     7921 atttactaaa actaccatat gcttgttcaa tttttaaata cccttgagct tgattaacaa
     7981 ttgcttctaa ttttttacga tgatgaacga tatttggaaa agtcattaag cggtcgatat
     8041 cttgagcggt catttgtgct accttttctg gttcgaaatc ataaaatgct tcttcatagg
     8101 cttctttctt ttttaaaata gttaaccaag atagcccagc atgttgtgat tctaatgcta
     8161 aaagtttaaa caatgccttg ctatcataga gcggttgtcc ccatacatga tcatgatagt
     8221 ttaagtagac tggatcttta gtaccaaatg cgcattcatt cattttattc ccacctatac
     8281 attgacttat tttgtcaatc attatactat aagttaagag tttaatatag gatttgggaa
     8341 gagtaagttc aaattatttt aaagagagca tatgatttgg tgtgagtatg taaatgattt
     8401 ttactgaagg tagcccacag agtatcttag ctgaacgtga ttaatagtag gctaagacgt
     8461 gtttgagcgt taatcataat ctagagtgct tgaagagatg aagcatttca aatttcacga
     8521 tgatagtatt ggtatgatta ttccaattat gttaaaagag tgaatttgat attaaacatc
     8581 cttcttgaat ataggtggta ccacggaata tccgtcctat ttgtatatag gatggatatt
     8641 tttatttttt taggaggtat agcaaatgga aatgaaacca aaatatgatc ctcgtgaagt
     8701 tgaagcggga cgttatgaag aatgggtaaa gaatggttat tttaaaccgt cagaagataa
     8761 atcaaaagaa acatatacaa ttgttatccc gccaccaaat gtaactggta aattacattt
     8821 aggacatgca tgggatacga ctttacaaga tatcattaca cgtatgaaac gtatgcaagg
     8881 atacgatacg ttatacttac caggtatgga tcatgctggt attgcgacac aggcaaaggt
     8941 agaagctaaa ttaaatgaac aaggaataac tagatatgat cttggtcgtg aaaagttttt
     9001 agaacaggca tgggattgga aagaagagta tgcgtcattt attcgtgcgc aatgggctaa
     9061 attaggtcta ggtttagatt atagtagaga acgttttact ttagatgaag gtttaagtaa
     9121 agcagttaaa aaagtttttg ttgatttata caataaagga attatttatc gtggcgaacg
     9181 tattataaat tgggatccta aagcacgtac agctttatct gatattgaag taatacatga
     9241 agatgttcaa ggtgcgtttt atcattttaa atatccttac gctgatggtg aaggttttat
     9301 tgaaattgca acaacaagac cagaaacgat gttaggtgat acagcgattg ttgttaaccc
     9361 taatgacgaa cgatacaaag atgtaatcgg taaaactgtt atattaccaa tcgtaggacg
     9421 cgaactgcct attttagcag atgagtatgt tgatatagac ttcggttctg gtgctatgaa
     9481 agtgacacca gcacatgacc ctaatgattt tgaaattggt caaagacatc aattagaaaa
     9541 tattatcgtt atggatgaaa atggtaaaat gaacgacaaa gcgggtaaat atgaaggtat
     9601 ggaccgtttt gattgtcgta aacagctagt taaagattta aaagaacaag atttagttat
     9661 caagattgaa gatcatgttc attctgtagg tcattcagaa cgatctggcg ctgttgttga
     9721 accatattta tcaacacaat ggtttgtgcg catggaagac ttagcgaaac gttcattaga
     9781 taaccaaaaa acagatgatc gtattgattt ttatccgcaa cgtttcgaac atacatttaa
     9841 ccaatggatg gaaaatatta gagattggac gatttcaaga caattatggt ggggtcatca
     9901 aattccggct tggtatcata aagaaacagg cgaaatatat gttggagaag aagcgccaac
     9961 tgatattgaa aattggcaac aagatgaaga tgtattagat acgtggttct caagtgcttt
    10021 atggcctttc tctacgttag gttggcctga tttagaaagt gaagacttta aacgatacta
    10081 cccaacaaat gccttagtta caggttacga tattatcttt ttctgggtag cacgcatgat
    10141 attccaaggc ttagaattta cagatcgtcg tccatttaat gatgtattat tacacggttt
    10201 agttcgtgct gaagacgggc gtaagatgag taaatcatta ggtaatggtg tggatccaat
    10261 ggatgttatt gacgaatacg gtgctgatag cttgcgttac ttcttagcaa caggttcatc
    10321 tccaggacat gatttaagat actcaactga aaaagttgag tcagtgtgga actttatcaa
    10381 taaaatctgg aatggggcac gtttcagttt aatgaatatc ggtgaagact ttaaagttga
    10441 agatatcgat ttaagtggta acttatcatt agcagataaa tggattctaa cacgtttaaa
    10501 tgaaacgatt gcaacagtta ctgatttaag tgacaaatat gaattcggcg aagttggacg
    10561 tgcattatat aatttcattt gggatgattt ctgtgattgg tacattgaaa tgagtaaaat
    10621 tccaatgaat agtaatgatg aagaacaaaa acaagttaca cgttcagtat tgagttatac
    10681 tttagacaat attatgagaa tgctacatcc attcatgcca tttgtaacag agaaaatatg
    10741 gcaaagttta ccacatgaag gtgacacaat tgttaaagct tcatggccag aagtgcgtga
    10801 atcattgatt tttgaagaaa gtaaacaaac aatgcaacaa cttgttgaaa tcattaaatc
    10861 tgtaagacaa tcacgtgtag aagtaaatac gccattgtct aaagaaatac ctattttaat
    10921 tcaagctaaa gataaagaaa ttgaaacaac tttatcacaa aacaaagatt atttaatcaa
    10981 attctgtaat cctagtacct taaatattag cactgacgtg gaaattcctg agaaagcaat
    11041 gacatcagtt gtaattgcag gtaaagtggt attaccatta gaagggctaa ttgatatgga
    11101 taaggaaatc agccgtttgg aaaaagaatt agctaaactt caaagcgaat tagatagagt
    11161 agataaaaag ctctctaatg aaaactttgt aagtaaagca cctgaaaagg ttataaatga
    11221 agaaaaacgt aaaaaacaag attatcaaga aaaatatgat ggtgtgaagg caagaattga
    11281 acaattaaaa gcataggagt tagtaacaat gaattaccta gagagcttgt attggataca
    11341 cgaaagaact aaatttggca tcaaaccagg tgttaaacgt atggaatgga tgctagcaca
    11401 atttaataat cctcaaaata acattaaggg tattcatgta ggtggcacaa atggtaaagg
    11461 ctctacagtt gcttacctta gaacagcttt agttgaaaat ggttatgaag taggtacatt
    11521 tacgtcgccg tttattgaaa catttaatga acgaattagt ctaaatggtg tgccaatatc
    11581 aaatgacgct attgtagaat tagtatcacg tattaaacca gtaagtgaaa tgatggaacg
    11641 tgaaacagat ttaggtgttg caactgaatt cgaaataatc acagcgatga tgtttttata
    11701 ttttggtgaa atacatcctg ttgattttgt cattgttgag gctggattgg gtataaagaa
    11761 cgattcgaca aatgtcttta caccggtttt atcaatctta actagtatcg gtctagacca
    11821 tacagatatt ttaggtggta cttatctaga tattgctagg gataaaggcg cgattataaa
    11881 gcctaacgtt ccagtgatat atgctgttaa aaatgaagat gcattaaaat atgttcgtga
    11941 acgcgcaatt gaacaacatg caaagccaat tgaattagat agagaaattg ttgttgtatc
    12001 gcaaaatgat gaatttactt accgttataa agattatgaa ttagaaacaa tcattttaag
    12061 catgttaggt gaacatcaga aacaaaatgc tgcattagcc ataacagctc ttattgaatt
    12121 aaatgaacaa ggattaattg aattagattt caataagatg atagacggta ttgaatcagt
    12181 tcgttggact ggacgtattg agcaggtgca tgacaaacct ttaatcattt tggatggcgc
    12241 acataattca gagagtatag atgctctaat tgatacaatt aaacagtacc atgataaaga
    12301 aaaagtagat attttgttct cagcaataaa cggaaaaccg attaacgaga tggtcaaaca
    12361 tttaagttta attgcgcata cgttttatgc aactgaattt gattttccga aagcgttacg
    12421 caaagaagaa attgtaggta gtattgaaaa tgatgaaata caattagtag atgactacgt
    12481 tgaatttata aaaaattatc aaggtgatac attagtaatt accggtagtc tgtatttcat
    12541 aagtgaagtt aaatcaaaat tagactttga agcattataa aactaaaact aaaatagcca
    12601 tgaagaatct atttaatcaa taggtagagc ttcatggtta ttttttatgc tctaaatcgt
    12661 atgtgtttat aaggttcaat ttcttgccat tattaatttt gccgagcagt aatgggttgt
    12721 taaagtatcc atcaattatg cttagcaata ttgaaaaatt taaatgaaaa agcggttcaa
    12781 ttcttgaaaa atagtgtata tttacatttt ggtaataact aactataatt tacataagga
    12841 ggttgtcatt tggtagtatt gttatcttat agttgcagtt gtatttttag ttttctatat
    12901 caatttatat ctatagaaga aacgtcattt gattatttac atagaagatc gaaatgtgat
    12961 tattgtaatt cgtcactcaa atggtatgaa ttaatgccga ttattagttt tttattatta
    13021 aaagggcgat gtcgaaactg tcgaaagcgt atttccctaa cacatttctt aggggaaacc
    13081 tttgctttaa tacctatcgt ctttattaag tatgatttca catacgtaaa tgctacgcta
    13141 tttataacta catatgtttt tctgcttatt tttactatga ccgatatcac ttctttaatg
    13201 ttagattgtc gcttaattat aatttattgt atcgtttctc tctcgttaag tatgatttat
    13261 ccagtagctt ttatcattat tagtatgacc acgcatatat tctacttttt atttcgggca
    13321 tatattggtt atggtgacgt tttactaata tctgcacttt ctttgttttt ccctctccaa
    13381 ttcactattt atgtcatttt atttacattt gtcattgctg gtttagttgc tttaattacc
    13441 atgatattta agccgattaa actattaccc cttgttccat ttatatttat ttcctttttt
    13501 atcaattcac ttttttataa tgatatccat caatttttag gaggcgtata tttttgaaaa
    13561 ttaaagaaat ggtaacttca gaaatgccaa gagaacgttt gttaagccat ggtgcaaaaa
    13621 gtctttcgaa tacagaatta ttagctatat taattaacac cggaagaaaa ggattctcga
    13681 gcatagacat tagtaatgaa ctgcttaaat ctgcttcgaa tctgaatgaa ttgaaaaaat
    13741 cttcaattaa cgatttgata aaagttaaag ggattggatt acaaaaagca attactttaa
    13801 aagcagcatt tgagttaggg gaaaggatgg ggagaagagc tgaaaataat cgtataaaaa
    13861 taacgcaacc aagtgatgtt gctgattata tgattccaac aatgaaagat ttaacacaag
    13921 aacattttgt cattttattg ttaaattcaa aaaatgtagt gattaaagaa acctgtgttt
    13981 ttaaaggtac attaaatagt tcgattgtac atccatgtga aatttttagt attgcggtga
    14041 gagaaaatgc caatgcaatc atcgcagttc ataatcatcc atccggtgat gtaacgccct
    14101 cacaagaaga tatcataaca acaatgaggt tgaaggagtg tggtttgatt ttagggatag
    14161 atttattgga tcatattata atcggtgata atagatttac cagtcttgta gaagcgggtt
    14221 actttgatga aaatgattga agttgataga attaatgacg tatcttgtgt ataataccta
    14281 cgaagtactt tcattggagg aaaaatagtg actctattta ttattatcgg ggttctcgtg
    14341 ccaatggttt ataccatgca gttaaatatt aaaaatgaac ctgtaacaaa gcgcaatcta
    14401 ttaataacat tagctttatc tacgttaggt attttagtaa cggcgttagc aggtgtgatc
    14461 gttacgaaac aagcttttcc tttattaagt gtagcaatcg gctcaatttt tactggaatc
    14521 gtttggggcc ttttactaag tggtagctac gcgctgatac gatttttatc taacgcattt
    14581 gggcgtaagt aatagtaatc tgttcatcaa gtagtatccg tgcttgaaac caaattaaaa
    14641 ctcctaatgt ggaactagtt aaaaaattct aaactagtac attaggagtt ttgttatgca
    14701 gaataaattt aattgttaaa ttgaaagtgc ggtaaaaacc cactatttat ttgaaaaaaa
    14761 tcgagcaacc aaattaaacc ttgatatcct aagtaaatac ataataaaac aagtccgaca
    14821 taaattaaaa atcgcaaaat agaaagtcca actctaaaaa ggaggatgac taatagtgct
    14881 attaaaataa ttgttagtat actcaatgct acaaacctcc tcatacgctt ttaaatcata
    14941 acaaaattat aagctatttt aaatgaacgt gcatcagtcc taagatatat catgattaag
    15001 gctgaagact gaggaaaata tacataataa aatatataat tagatgaaga cattggaggc
    15061 gaactatgag attgatattt agtatgatta aaaacattat agcagtaata gcaattgtga
    15121 taattgttta tatcgccttg aaatacgcac cttttttaaa agaacaagat tggaatcctg
    15181 taaatcacga taatgatcaa atgaatcaag ttacgcaacc cactaatgat gcccaacatg
    15241 tttatgtaag tggtgagaaa tattcattaa aagaaaatga tttaataaag aatgtaccat
    15301 taagccaaat taaaaatgta tttaaaatga tagataaaca agagtttatg gctgtgtctg
    15361 gaatgaatcg catggcttat aatgatcaat atattatagg tcaaagagga gacgaattta
    15421 ttctttataa atttggagat gagtcaatgc gtgtttacaa tactgaattt gaaatgcaac
    15481 aagacttaaa tgaattaggg caaaatttac aactaaaacc cgaaaatgct tatcaatagg
    15541 aaattattgc ataagaaata tattttagtt atcctgtcac tagaataaat ttaaaaggtt
    15601 ggtgtagttc aaatggcaga acaatcaaaa cagaaacaag ctaatgaaca acaaaaggcc
    15661 cagaacttat tcgcacgttg gagacaactt cagaattcga acagtgaatc ctcgaacgat
    15721 acaaacaaat aattttaaaa tttaaatcga ttatttaaac tactagtgac tggaatgttt
    15781 gctagtagtt ttttgttttt aaaatgccac aaatttttta cggagttgtt cattttcaaa
    15841 gtaattcgta gttagtgctt tatttcataa tgggagtaat ataaaataga aaaggatata
    15901 caatattttg acataataga ggtgttctgg gtgcttaagt tttttaaaaa taacaaatta
    15961 attgttgttt tatgtgcaat tatcgttttt attgcattaa ttgggctgtc catacgttca
    16021 caatctcaat cacctcctga acaatatata ggtgattctg tgtcttttgg acaacgagtt
    16081 gtgagttatc cagttaattt tgttgctggt acgattgggg acttttttaa aaaaggagat
    16141 tctaaagaat ctaaaaataa gattagccag ttagaatcta agaaccaaca attagaagcg
    16201 gaaaatgaaa aattaaaaaa agagcttgat ttaaaagata tttcaaaatt tgatcctatt
    16261 tctactacgg ttttagcaag aaatccggat cagtggatga atacaattgt aattgataag
    16321 ggatctaaat caggtataac ttcaaatatg gctgtgatga catcacaagg ttttgttgga
    16381 agagttacta aagttaataa attttcttca caagttgatt taatctcaac taatacacgt
    16441 gcgggtaaat tatctgtaaa tatacaacac ggttctaaaa atatatttgg tttaattgat
    16501 cgttatgatg aaaagaactc agaacttgta attagtgaca ttaataatag agataatatc
    16561 tcaaaaggtg ataaagtcgt tacaagtgga ttagctgatc aactaccaag taatttatat
    16621 ataggagaag tgactaaggt tcaaaatgat caatacggct tagctaaaga agttagggtt
    16681 aagactggtg cagacttaac agatttgagt catgtttatg ttgcaaaaag agatcctaaa
    16741 acaattcctg atgatgaaag cagggataaa taatgcgtac actgtattat tttttgatag
    16801 gtattttact attttatata gatactgcaa ttgggcttct tattccaatg catataggta
    16861 agtttgaact tgtatttgta ccacacctta catttatgta tattttaatg atggtagtgt
    16921 atcgtggctt tggcgtatca ttattgctca gcatattttt aggcgtaatg actgatgtat
    16981 actttggtag tatttatgga gtgtacttat ttggctatat attgttttta gcacttatag
    17041 atcgattctt taaaaatttt tacaaagatc attcgatgtt attcattatt atattagcca
    17101 gcaccttatt attagaagtc tatgtggcat taatatacgg tatgttagga ttcattcaat
    17161 ttgatattat tcattttgta gtctttagat tattgccaac attaattatg aattttgtac
    17221 tgttgattat gctttatccg ttgattataa agttccttaa aaaaacaaac aatgacattg
    17281 acatgaaacg tcgtcaatgg taagatgcaa tagttgagta gttataagta gctacataca
    17341 accgctcaaa tataggttca agaacatttt taatgaagaa agcgattgtc aatcaaggcg
    17401 ttgaaggaag aaacgagaag agtttatgtt tataaatgac tcgagttgcg accgaaacaa
    17461 cgtagagtgc aaacgcttga ctcaataacg aaatgtcacc tatgtatggc gtgacttatt
    17521 atacaggagg tgcaaagtat gtttgctatt attgaaacag gtggaaaaca aatcaaagta
    17581 gaagaaggtc aagaaatctt cgttgaaaaa ttagacgtaa acgaaggaga tacttttaca
    17641 tttgataaag tattatttgt aggtggagat tcagttaaag ttggagcgcc aacagttgaa
    17701 ggtgcaacag ttactgctac tgttaataaa caaggtcgcg gtaaaaaaat cactgtattc
    17761 acatacaaac gtcgtaaaaa ttcaaaacgt aaaaaaggcc atcgtcaacc atacactaaa
    17821 ttaacaatcg ataaaatcaa cgcgtaatta ttatgattac tgttgatatt acagttaatg
    17881 atgaaggcaa agtaacagac gttattatgg atggccatgc tgaccatggt gaatatggtc
    17941 atgatatcgt ttgtgctgga gcttcagctg tattgtttgg tagtgttaat gcgattatag
    18001 gattgacatc tgagagacca gatatcaatt atgacgacaa tggtggtcat tttcatataa
    18061 gaagcgttga tacaaataac gatgaagcgc aactaattct tcaaacaatg cttgtgtctt
    18121 tacaaactat tgaagaagaa tataatgaga atattagatt aaattataag tgaggtgcat
    18181 tccgatgtta aaattaaact tacaattctt cgcatctaaa aaaggggtaa gttctacaaa
    18241 aaacggacgt gactctgaat caaaacgctt aggtgctaaa cgtgctgacg gtcaattcgt
    18301 aacaggtggt tcaattttat atcgccaacg tggtactaaa atttaccctg gtgaaaatgt
    18361 aggtcgtggt ggcgatgata cattattcgc taaaatcgac ggcgttgtta aattcgaacg
    18421 taaaggtcgc gacaaaaaac aagtttctgt atatgcagta gctgaataat tttgtctagt
    18481 taacaccaga agtgaatctt ctggtgtttt ttacttttta taaaaggttt ttcattatta
    18541 ttttaattat gttattaaaa aatgggaatt ttagacgtta tactaaatgt
//
LOCUS       DAHHYJ010000041        18482 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000041 DAHHYJ010000000
VERSION     DAHHYJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18482
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.041"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            35..433
                     /locus_tag="NBK22_001067"
     CDS             35..433
                     /locus_tag="NBK22_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD2414263.1"
                     /translation="MEKSIKIMTIIGIVIQGLATVFSLLLMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLSLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLVSGIKLLTNNKPKDEISDLS"
     gene            complement(648..1472)
                     /locus_tag="NBK22_001068"
     CDS             complement(648..1472)
                     /locus_tag="NBK22_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD2414264.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(1711..3018)
                     /gene="brnQ"
                     /locus_tag="NBK22_001069"
     CDS             complement(1711..3018)
                     /gene="brnQ"
                     /locus_tag="NBK22_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD2414265.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNIV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALVFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKWFMMLPLADIDLAWLVPFMLLAIIGFIIDVVIRRPKQATT"
     gene            3602..4492
                     /locus_tag="NBK22_001070"
     CDS             3602..4492
                     /locus_tag="NBK22_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCD2414266.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKANLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            4742..5791
                     /locus_tag="NBK22_001071"
     CDS             4742..5791
                     /locus_tag="NBK22_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414267.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNIL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDSNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            5804..6481
                     /locus_tag="NBK22_001072"
     CDS             5804..6481
                     /locus_tag="NBK22_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414268.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKKRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            6689..7720
                     /locus_tag="NBK22_001073"
     CDS             6689..7720
                     /locus_tag="NBK22_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD2414269.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPMTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            8063..9181
                     /locus_tag="NBK22_001074"
     CDS             8063..9181
                     /locus_tag="NBK22_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD2414270.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            9156..10079
                     /locus_tag="NBK22_001075"
     CDS             9156..10079
                     /locus_tag="NBK22_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCD2414271.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATNKDVAKVSRRDLAEVIAMK
                     CIGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            10090..11310
                     /locus_tag="NBK22_001076"
     CDS             10090..11310
                     /locus_tag="NBK22_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD2414272.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKNIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     SDVEIDNLTKSTETKSVNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(11416..12948)
                     /locus_tag="NBK22_001077"
     CDS             complement(11416..12948)
                     /locus_tag="NBK22_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCD2414273.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYAHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARLVIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(12988..13869)
                     /locus_tag="NBK22_001078"
     CDS             complement(12988..13869)
                     /locus_tag="NBK22_001078"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCD2414274.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIQLGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            14027..14887
                     /locus_tag="NBK22_001079"
     CDS             14027..14887
                     /locus_tag="NBK22_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD2414275.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQIPTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQGHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(15164..15964)
                     /locus_tag="NBK22_001080"
     CDS             complement(15164..15964)
                     /locus_tag="NBK22_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD2414276.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            16104..16772
                     /locus_tag="NBK22_001081"
     CDS             16104..16772
                     /locus_tag="NBK22_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCD2414277.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     VMED"
     gene            complement(16899..18212)
                     /locus_tag="NBK22_001082"
     CDS             complement(16899..18212)
                     /locus_tag="NBK22_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCD2414278.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQAVLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         6467 a         2853 c         3412 g         5750 t
ORIGIN      
        1 gtaagtttgt aaataataat aaaggagtaa gtcaatggaa aaatcgatca aaataatgac
       61 aataatagga attgtgattc agggtttagc aacggtattt agtttactat tgatggtttt
      121 agcagcatca ggtgtaatga ctacagatgt gtcaacaaca gttaatggtg aggttgaccc
      181 agttgatgca gaaacagcag cagcaatttt cactgtatta ttcttatccc tattcatatt
      241 tggaatcatt tcaattattt taggtgcaat cggtatgttt aaagcatcta aaaacaaaaa
      301 aatgagtggt atattgttga ttattggagc tgtaataagt ggtaacataa ttacatttgc
      361 tttatggtta gtcagtggta ttaaactcct tactaataac aagcctaaag atgaaataag
      421 cgacttatca taaacatcgt atattgaaat ttcaagattt cttaagtaat taaataaagt
      481 gctctcttag aagtagattt tcagtcaata actgctttta agagagcatt ttgtatttga
      541 taatgctcat agcatcggat aaaaaccatt gacgaactta aaaaaatacg ttagacaatg
      601 cacttctaaa tattaaaggc atcgactaac gcattgttca aatatagtta attattttta
      661 taaaattgat gatgatcatt caagtaagca tagaataaac ctataatgag tccgcctcca
      721 atatagttac cgataaaagc cgcagcgata ttcgaaatag ctggtatgaa gtgcaatgta
      781 tcaacttgat aaattaaacc acccataaat aagcaactgt tgtaaacgac atgttcataa
      841 cccataaagg cgaatatggt aacaccgaac atcatgacaa acatttttgc aagtacatcg
      901 tcaatttgca tggcaataac taatgaaata ttgataaaga aattggcgaa tatcgctttc
      961 attaatatac ttacaaaacc agtagacaac gttttatgct ctataactgc tgataactga
     1021 tttaacatat ctggcgtcat tacatttgaa aaacgcatga aactaaataa aatagcagca
     1081 cctaaaatat ttcctgcaaa gcataataaa aatattttca atactctagt tggtttaatt
     1141 actttataat acaggcctac agtaaagtac atgaagttac tggttagtag ttcggagttt
     1201 gtaaataaaa tgagtactaa cgcaaagctg aatgtaatgg cactggccat attcacaatg
     1261 cctggcggta aatctggttc gtgtgttgct ttaactgata atacgaagac cgtaataatc
     1321 ccgataataa atcctgccat catcgcgcgt aataaataac gttttaaata aacgctttgt
     1381 aatatatctt ttgttcttat tgtttcgact acgttattta cccagtcgtc cccataaaat
     1441 atcttatccc atttaatatg tttctccttc acaatgacat cctcactttt catttgttaa
     1501 tcagagtata ctatgaatcg ccttatttgt gaataatttc acaaaattaa ttttaaaaac
     1561 taatattttt gaataactga taggattttt tggcaaatca attattttta aaataatcaa
     1621 tagacagaca gtatcgctga aaatccatga atttcatata aaaaagccta gtaatgatgc
     1681 gacgatcatt actaggagat tatgcgtcta ttaagttgtc gcttgtttcg gacggcgtat
     1741 aactacatcg atgatgaaac ctattattgc caagagcatg aatggtacaa gccaagctaa
     1801 atcgatatct gctaaaggta acatcataaa ccatttcaaa ataacaccgt gtaataagtt
     1861 gaaactattt agtatttgta aaattgaaat aatcaatgta ataacagttg cgagtcgata
     1921 ggcccaactg aatctgaatg tgctaaacat gttagcaaat gatatcagta caagtgcaat
     1981 agacacggga tatattaatg tcaataatgg gacagcaatt tttaaaatca tttctaaacc
     2041 aagtgttgta aataagaacc ctatgataga gaaaacaagt gcgaatattt tataagaaaa
     2101 cttaggtacg tgtttcttag taaatgttgc acaagcgttg acgagtccta tacatgttgt
     2161 taggcatgca agaatcactg tcataccaaa tactatgttg ccaaatgaac caaatactcg
     2221 taatgagttg tacgtcaata tatctgtacc atctttaaag tttcctggag ctgttgatgc
     2281 acctacatat gccaatgcaa agtaaatcat accaagtaat atagctgcaa taagacctga
     2341 aaagcagaca tattttaaaa ttttcatgcg atctgtgagg cctttaaact tatagccatt
     2401 gacaatgact acggaaaaag ctaacgcagc aacaagatcc attgtaaaat agccttccaa
     2461 acttcctgaa atgaaaggat gtgttatata tttatcctta ggtgcactta gtgcagattc
     2521 agggttgaaa atgacagcaa tacttaatag agcgaccatt aatagtaata acggtgttaa
     2581 taatttacct aaattatcaa cgattttcga tggatttaaa ctaatccagt aaacgatggc
     2641 aaaaaagatt gctgcgaata taattaaagt ccattggtta tgcacaggta aaatgtgtct
     2701 tgtaccaatt tcgtacgcga catttgcagc acgtggaata ccgtaaaatg ctccgataga
     2761 catgtaaatc acgacagcaa aaataaaccc gaaccatgga tgtatacgat tgcctacact
     2821 ttcaacacct tcatcataaa atgcaacaac aataacagta ataaagggga gtaatatgcc
     2881 tgtaagggca aagcctagca taccaatcca catattttga cccgctgtat ggccaagcat
     2941 gggcgggaat attaaatttc cggctccaaa aaatagtgaa aataacatga ggcccgaaat
     3001 aataacttgt tttttcaatg taatttcctt cctaaaacta gatattcata ataatttaaa
     3061 aaatctgaaa aataaaaacg ttcttacttt atctttaaat gacaatacta tgattctatt
     3121 attttataaa aaagattgca ataataacgt cttgctatta ttggataatg acaattaaag
     3181 tgtcgttttt atggattaaa ataggtgaaa gtaagttgga aatacgttgg agcgttgtga
     3241 tttttatgag aaattgtatg agtgaggact gctatttata aaaggtaaac tatggatgag
     3301 tttatggttg ttccaatagg taaataagag atagcacact gtacacataa tgatacgtgg
     3361 atgagtgaat atgtctgata ggaaggccta gtcactcaaa tgaatttaag tttattacgt
     3421 gataaatcac aaatctctct catgtgatag gtctcccatt aaatcatgct tatatgaaat
     3481 gttcacatat ttgttagctt ttcaagaaat aatattaaat atcttttcat agtatagcaa
     3541 tattaaataa atgcatttat aattttaact gtattttaaa tattaatcat gaggtgataa
     3601 gatgaataaa atttcaaagt atattgcaat agcatcatta tcggtagcgg ttacagtttc
     3661 agcgccacaa acgacaaatt ctacagcgtt tgccaaaagt tctgctgaag ttcaacaaac
     3721 gcaacaagct tctataccag catcacaaaa ggcgaatctt ggtaatcaaa atattatggc
     3781 agtggcttgg tatcaaaatt cagctgaagc aaaagcatta tatttacaag gttataacag
     3841 tgcaaagact cagttagata aagagattaa aaagaataaa ggtaaacata agttagctat
     3901 tgctttggat ttagatgaaa cagttttaga taattctcca tatcaaggct atgcatcaat
     3961 acataataaa cctttcccag aaggttggca tgaatgggta caagctgcta aagctaaacc
     4021 tgtctatggc gcaaaagaat tcttgaaata tgctgacaaa aaaggtgtcg atatctacta
     4081 tatttctgat agagataaag aaaaagattt aaaggcaaca caaaagaact taaaacaaca
     4141 aggtatccct caagctaaga agagtcatat tttactaaaa ggtaaagatg ataagagtaa
     4201 agaatcacgc agacaaatgg ttcaaaagga tcataaactt gtcatgctat ttggagataa
     4261 tttattagac tttacagatc caaaagaagc tacagctgaa tctcgtgaag cattaattga
     4321 aaaacataaa gacgatttcg gtaagaaata tatcattttc cctaacccaa tgtatggtag
     4381 ttgggaagct acaatttaca acaataacta taaagcaagt gacaaagcaa aagataaatt
     4441 acgtaaaaat gctattaagc aattcgatcc taaaacaggc gaagttaaat aatatatgaa
     4501 ttggacgtct acatgtactt aaaggtatat gtaggcgttt tttaattgat ccttaaattt
     4561 taaatgtacc gtacaaaaat aatagacaat taattgcatt ttccataaat ttgcttttaa
     4621 tataaaaagt tcgggtataa tgaaggataa gtgtatgtat aacattacag agcgcaacac
     4681 gacgtacgtg catctaagaa tataaacaat gtttaaatca tagtagtagg gagagaaatg
     4741 catgttttta gcttggaatg aaatacggcg caacaaattg aagtttggac taattattgg
     4801 tgtgttaacg atgattagtt acttgctatt tttattatct ggattggcga atggtcttat
     4861 caatatgaat aaagaaggca ttgataagtg gcaagcagat gccattgttc taaataaaga
     4921 tgccaatcaa actgtgcaac aatctgtttt taacaagaaa gatattgaaa ataaatacaa
     4981 gaagcaagct actttgaagc aaacagggga aattgtgtct aatggccatc aaaaagacaa
     5041 tattttagtg ttcggtgttg aaaagtcatc atttttagtt ccgagtttaa tagaagggca
     5101 taaagcgact aaagataatg aagtgttagc tgatgaaaca cttaaaaata aaggatttaa
     5161 aattggcgac acattatcac tatctcaatc agatgaaaaa ttgcatatcg taggttttac
     5221 agaaagtgca aaatataatg cgtcaccagt cattttcacg aatgacgcta ccattgccaa
     5281 gatcaatcct agattgactg gagataaaat taatgcagtt gttgtacgtg attcaaattg
     5341 gaaagacaaa aaattaaacc aagagcttga agcggtaagt attaatgact ttattgaaaa
     5401 tttaccaggt tataaaccgc agaacttaac attaaacttt atgatttcat tcttatttgt
     5461 catttcagct acagttatag gcattttctt atatgtcatg acattacaaa aaacaagttt
     5521 atttggcata ttaaaggctc aaggatttac gaatggctat ttagcgaacg tagttatttc
     5581 gcagacgctc atattagcat tatttggtac ggcatttggc ttactgttaa caggcgttac
     5641 aggtgcattt ttacctgatg cagtacctgt caaattcgat gtactaacat tgctcgtatt
     5701 tgcaattgtg ttaatgattg tctctgtatt aggaagttta ttctccattt taacaattag
     5761 aaaaatagat ccgttaaagg cgatcgggta ggaggtgtag caaatgttga aatttgaaaa
     5821 tgtaacaaag tcatttaaag atgggaatcg taacattgaa gcggttaaag atacaaattt
     5881 tgagataaat aaaggtgata ttatagcatt ggttggacct tctggctctg gtaaaagtac
     5941 atttctaact atggcaggtg ctttacaaac accgacatct gggcacattt taatcaataa
     6001 ccaagatatt acgacaatga agcaaaaagc attggcaaaa gttagaatgt ctgaaatagg
     6061 ttttatttta caagctacaa accttgtacc atttttaacg gtaaagcaac aatttacatt
     6121 attgaaaaag aaaaataaga atgttatgtc taatgaagac tatcagcaac ttatgtcaca
     6181 attaggtcta acatcattgc ttaataagtt accttcagaa atttcaggtg gtcagaaaca
     6241 acgtgtggcg atagccaaag cgttgtatac gaatccgtcg attattttag cggatgaacc
     6301 taccgcggcg ttagatactg aaaatgcgat tgaagttatt aaaattctac gtgatcaagc
     6361 caaaaaaaga aagaaagcat gtattattgt tacacatgat gaacgactta aagcatattg
     6421 tgatcgttca tatcatatga aagatggcgt ccttaatctt gaaaatgaaa cagtagaata
     6481 gttttattaa gccggtacat catgtgccgg tatttttatg tttatgtatt atttgaataa
     6541 actttcacat tcaattaata ataattatta tcggaaatta gaaatattcc gtgaaatata
     6601 atattttttg tagtaaaatg gcctctaagt attcaatatt taaatatggg gattgaatat
     6661 aaaattatcg taatgggggt caatggttat ggatttattg ataggtactt tatttttatt
     6721 tttggtctta gtgattttta cattatttac atataaagcg cctaatggta tgcgtgccat
     6781 gggagcatta gctaatgcag caatcgcaac atttttagta gaagcattta ataaatatgt
     6841 tggtggcgaa gtattcggta ttaaattttt agaagagcta ggagacgctg cgggaggtct
     6901 aggtggtgtc gctgccgctg gattaacagc attagctatc ggtgtgtcac cagtatatgc
     6961 attagttata gcagccgcgt gcggtggtat ggatttatta ccaggtttct ttgcgggtta
     7021 tatgattgga tatgtgatga aatatacaga gaaatatgtg ccggatggtg tcgacttaat
     7081 tggatcgatt gtcatcttag caccattagc tcgtcttatt gcagtattat taacgccagt
     7141 agtgaatagt acattgattc gaattggtga tattatccaa agtagtacga atacgaatcc
     7201 aattatcatg ggtatcattt taggtggtat tatcacggtt gtcggcacag cgccattgag
     7261 ttcaatggca ttgacagcat tattaggttt aacgggtgta cctatggcta ttggtgccat
     7321 ggcagcattt agttcggcat ttatgaatgg gacgctattc catcgcttaa aattaggtga
     7381 tcgtaagtct acgattgcag taagtattga acctttatca caagcagata ttgtatcagc
     7441 caatccaatt ccaatctata ttacaaattt ctttggtggt gcgattgctg gtttaattat
     7501 tgctatgtca ggtttaatta acgatgcgac aggtacagct acaccgattg caggattttt
     7561 agttatgttt ggatttaatc atccaatgac tattgtaatt tatggtgtag taatggcgat
     7621 tgtaggtgcg cttgcaggtt atcttggttc aattgtattt aaaaaatatc caattgttac
     7681 taagcaagac atgattaatc gaggtgcagt agacgcatag catcatcata ttgaatagta
     7741 aaaacaaata aaacatagta acgtgattca gtcgatgtaa cagtcgataa tgagtcacgt
     7801 ttttttatag aaaaatataa gacataaaaa tgtcataatt tatagtcgac aaatatcata
     7861 ctgtataaac atttatcatt ttctcaagta ccttttacac gatggaatga acttactttt
     7921 tacgaaatta tgcgtatttt ataaacaaat atcattgata taacggtaaa tgtaagcgtt
     7981 tacaacagaa ataacagcat gctacgatat ttttgtaaat tcactgattc aagtatttta
     8041 agtcaatatg aggagggatg ttatgagcga ttctgagaaa gaaattttaa aaagaattaa
     8101 agataatccg tttatttcac aacgtgaact tgctgaggca attggattat ctagacccag
     8161 cgtagcaaac attatttcag gattaataca aaaggaatat gttatgggaa aggcatatgt
     8221 tttaaatgaa gattatccta ttgtttgtat tggcgcagcg aatgtagatc gtaagtttta
     8281 tgtgcataaa aatttagttg cagaaacatc aaatcctgta acgtcaacac gctctattgg
     8341 tggcgtagca agaaatattg ctgagaactt aggtaggctt ggcgaaacgg tcgctttttt
     8401 atctgctagt ggacaagata gtgaatggga aatgattaaa cgattgtcca caccatttat
     8461 gaatttggat catgttcaac aatttgaaaa tgcgagtaca ggttcatata cagctttaat
     8521 tagtaaagaa ggcgacatga catatggctt agcagatatg gaagtgtttg actacattac
     8581 gcctgaattt ttaattaagc gttcacactt attgaaaaag gctaagtgca ttattgtaga
     8641 tttgaattta ggcaaagagg cattaaactt cttatgtgcc tataccacga aacatcaaat
     8701 caaattagtt atcaccacgg tttcttcccc aaaaatgaaa aatatgcctg attcattaca
     8761 tgctattgat tggattatca cgaataaaga tgaaacagaa acatacttaa atttaaaaat
     8821 agaatctact gatgatttaa aaatagctgc taaacgctgg aatgatttag gtgttaaaaa
     8881 tgttattgtg acaaatggcg tgaaagaact catttatcga agtggtgagg aagaaatcat
     8941 taagtcagtt atgccatcaa atagtgtgaa agatgttaca ggtgcaggcg attcattctg
     9001 tgctgcagta gtgtatagct ggttaaatgg gatgtctact gaagatatat taatagctgg
     9061 tatggttaac gcaaagaaaa cgatagaaac gaaatataca gttaggcaaa acctagatca
     9121 acagcaactt tatcacgata tggaggatta taaaaatggc aaatttacaa aagtatattg
     9181 agtattctcg agaagttcag caagcacggg agaacaatca accgattgta gcattagaat
     9241 caacaattat ttcgcatggt atgccgtacc cacaaaatgt tgaaatggca acaacagtag
     9301 agcaaattat caggaataat ggtgccattc cagcaaccat agccattata gatggcaaaa
     9361 ttaaaattgg tttagaaagt gaagatttag aaatacttgc aactaataaa gacgttgcta
     9421 aagtatctag aagggattta gcagaagtta ttgcgatgaa gtgtattggt gctactactg
     9481 tagctacgac gatgatatgt gctgcaatgg ctggtattca attttttgtt acaggaggta
     9541 ttgggggcgt ccataaaggt gcagaacata cgatggacat ttcagcagac ttagaagaac
     9601 tgtctaaaac aaatgtcact gttatctgtg caggtgccaa atcaatttta gacttaccta
     9661 agacgatgga gtatttagaa acaaaaggcg ttccagttat tggatatcaa acgaatgaat
     9721 tgccagcatt cttcactcgc gaaagcggtg ttaagttaac aagttcggtt gaaacgccag
     9781 aacgacttgc tgacattcat ttaacaaaac agcagttaaa tcttgaaggt ggcattgttg
     9841 ttgctaatcc tattccatat gagcatgcct tatcaaaagc atatattgag gcaatcataa
     9901 atgaagctgt tgttgaagcg gaaaatcaag gtattaaagg taaggacgcc acaccgttct
     9961 tgttagggaa aattgtagaa aaaacgaatg gtaaaagttt agcagcaaat ataaaacttg
    10021 ttgaaaacaa tgcggcgttg ggtgctaaaa ttgctgtcgc tgttaataaa ttattgtagg
    10081 tgatgataca tgaatatttt attcgctatc acagggatag catttgcact atttgttgcg
    10141 tttttattca gttttgatcg taaaaacata gacttcaaaa agacgttaat aatgatattt
    10201 attcaagtgt tgatcgtgtt atttatgatg aacacaacga ttggtttgac aattttaact
    10261 gcactaggtt cattttttga agggctaata aatattagta aagcaggcat aaattttgtt
    10321 tttggagata tacaaaataa aaatggcttt acgttctttt taaacgtatt actgccatta
    10381 gtttttattt ctgtattaat aggcattttt aattatatta aggtattacc atttattatc
    10441 aaatatgtag gtatcgctat taataaaata actagaatgg ggcgcttaga aagttacttt
    10501 gctatttcaa cagcaatgtt tgggcaacca gaagtatatt taacaataaa agatattatt
    10561 ccaagattat ctagagctaa attatataca attgcgacgt ctggtatgag tgctgttagt
    10621 atggcaatgt tgggttcata tatgcagatg attgagccca agttcgtagt tacagctgta
    10681 atgttaaata tttttagtgc gcttatcatc gctagtgtaa tcaatcccta taaatctgat
    10741 gatagtgatg ttgagattga taatttaact aaatcaacgg aaacgaaatc agtgaatgga
    10801 aaaacaggga aacctaagaa agttgccttc ttccaaatga taggtgacag tgcgatggat
    10861 ggatttaaaa tcgctgttgt agtagccgta atgttgttag catttatttc attaatggaa
    10921 gcaattaata tcatgtttgg tagtgttggt ttgaacttta aacagcttat tggctatgtg
    10981 tttgcaccaa tcgcattctt aatggggatt ccatggagcg aagctgttcc agctggctct
    11041 ttaatggcga ccaaattaat tacaaatgag tttgtagcaa tgcttgattt taaaaatgtc
    11101 ctgggtgatg tatcagctcg tacacaaggt atcatttcag tttacttagt aagcttcgct
    11161 aattttggta cggttggtat catcgtaggt tcaattaaag gcattagtga taaacaagga
    11221 gaaaaagttg catcctttgc aatgaggttg ctacttggtt caactctagc ttcaatcatt
    11281 tcaggatcaa ttattggctt agtattgtaa attaatcgaa gtatctaaat taaattcata
    11341 acaacatcaa aaccccgtca ccaagttggc gcaacagcgc atcataactt agtgacgggg
    11401 ttttatcata acaatctact ttttcgtagc cgtttttgaa atgtatgttg atggtttatc
    11461 tttttcaaaa attgttaatc ccgttatatc ttttttatgt tttgaaggga caatgaagct
    11521 aagtatataa gcaaagacaa aagcaactgt aaatgaaatg gtagatacat agaaaggtga
    11581 gttacctttg ccaacaccat tatagacata agcaaagatg atacccaata ttaatccaca
    11641 gataacaccg aatgtattcg tacgtttagt gaaaatacca actgcaaata caccagctaa
    11701 tggaacaccg aataatccag ttacaaacaa gaataaatcc cataagtcat ttgaattaga
    11761 agcaattaag tatagtgaca ttccaaaacc gaaaatacct gcaatgatga tgaccaaacg
    11821 agcaaagtta acttcgtgtc gctcgctacc ttttccgaag aagcgttgct taatgtcgat
    11881 tgaaatacaa gcagatatag aatttaaact agatgaaatg gtagactgtg cagcggcgaa
    11941 aatggctgca ataagtaatc ctgcaacaaa tggtggcatc tcagtcaaaa tgaaatatgg
    12001 cactacagat gatgtattga agccttttgg taaaacagct tcatgtgcat aaaatgaata
    12061 caacattgta cccataccat aaaataaggg tgctgaaatt aaagctagga taccatttgt
    12121 ccataacgat ttatttgttt cttttaaact atcagaagct tgataacgct gcacgacgtc
    12181 ttgactcgct gtgtattgat acaagttgtt gaaaatattt cctaggaaaa taattggaat
    12241 ggcagctgcc gcagtattta gtttccaatt gtctgcacta attaattttt tgtgctcaat
    12301 cgcatctgca aagacagtgc caaaaccgcc tttaatgttc ataacaccta gaataatgat
    12361 aactaaagcg ccgcctaata aaatgacgcc ttgaatgaaa tcactccaaa ccacaccttc
    12421 gaatccacct aaaaatgtat ataaaataca tagtaaacca acgagtgatg caacgatata
    12481 agggttcatg tctgatacag atgtgattgc taatgttggt aagtagataa cgattgcaac
    12541 acgccctaaa tggtaaacga caaataataa tgagccaata acacgtatgc tagggccaaa
    12601 tctagcttct aaatattcat atgcagatgt tacctttaac tttttaaaga aagggacata
    12661 gaaataaata agtaatggaa taattgcgac gatagcaatg ttaccagcga tatatgacca
    12721 atctgttaaa aatgctttct ctggtgtcga cataaatgta atcgcactta acgtagtagc
    12781 ataaattgaa aagccaacta cccaagatgg caagcgacca cttgcggtaa agaaactatt
    12841 ggtactctgg ctcgcgcgct tggtaaaata aacgccaatg aacaacatag ctagtagata
    12901 aatgataacg gcaacccagt ttagtgtgcc aaatccaact tctttcatgg gcaacatccc
    12961 ctttacaatg tattgattct ttgatgtcta taaatcgtat tttgcaatga gttgatctaa
    13021 tgtttgtcga tgtgcttcgt taaaaggttt gaaaggtctt ttcggtaatc ctgcatcaat
    13081 gccacgatga cgtaatattt ctttcaatgt tggataaatc cccattgata acactgtttc
    13141 gataatgtcg tttgaatcat gttgtagttg gtaagcttct tgaatttgac cttgtcttgc
    13201 taagtcgaag atttttctag cgcggcgacc attaacgtta tatgtagaac caattgcacc
    13261 gtctacgcca gaaatcgtag cttgaactaa catttcatca aagccagata agattaattt
    13321 atctgggaat gcttttctaa tacgttcgag taggaagaag ttcggtgctg tatatttaac
    13381 accaacaatt ttttcgtgat taaatagctc gctgaattgt tcaatagaaa tattcacacc
    13441 tgttaaatct ggtattgcat aaataatcat attgttctgt gttgcttcga taatatcgaa
    13501 atagtaatct ctaatttctt caaaagtaaa tggatagtag aatggtgtta cggcagaaag
    13561 tgcatcataa ccgagttctg tggcatattt cccaagttga atggcttcat ttaaatctaa
    13621 cgaacctact tgagcaatca atttcacttt atccccaact gcctctttgg caactttgaa
    13681 aacttgcttc ttctgctctg tatttaataa aaagttttcg cctgagctac catttacata
    13741 aagaccgtct aattcttcag tttcaatggc attttgagca atttgtttga gtccttgttc
    13801 atttacttga ccattttcat caaaaggaac gagtaacgct gcatataaac cttttaaatc
    13861 tttgttcatt atgaagtccc tccaaaaatc atttgataat atagtttaca gctataattg
    13921 taaacgctat cataaaatgt aacaatatct ttttgaaaat tgtagtcata tttatgtata
    13981 attaatgaaa atgtttttca aaatcaatag aaatggagtg agtaaggtgt attacatcgc
    14041 aatcgatatt ggaggcactc aaattaaatc ggcagttatt gataagcaat tgaatatgtt
    14101 tgactatcaa caaataccaa cgccggacaa caaaagtgag cttattactg acaaagtata
    14161 tgagattgta acaggatata tgaagcaata tcagttgatc caacctgtca taggtatttc
    14221 atcagcaggc gttgttgatg aacaaaaagg cgaaattgta tacgcagggc caaccattcc
    14281 gaattataaa ggtactaatt ttaagcgatt attaaaatca ttgtctcctt atgtcaaagt
    14341 aaaaaatgat gtaaacgctg cattactagg cgaattgaaa ttacatcaat atcaagcaga
    14401 acggatcttt tgtatgacac ttggtacagg cattgggggt gcgtacaaga ataatcaagg
    14461 tcatattgat aatggtgagc ttcataaggc aaatgaagtt gggtatttat tgtatcgtcc
    14521 aactgaaaat acaacgtttg agcaacgtgc tgcaacgagt gcattgaaaa agcgcatgat
    14581 tgacggagga tttacgagaa gcacacatgt gccagtattg tttgaagcag ctgaagaagg
    14641 tgatgatatt gcaaaacaaa tattgaatga gtgggcagaa gatgtagcag aagggattgc
    14701 ccaaatacaa gtcatgtatg atccagggct tatattaatt ggtggcggta tatctgaaca
    14761 aggagacaat ctcattaaat atatcgagcc gaaagttgca cactatttac caaaagacta
    14821 tgtttatgca ccgatacaaa cgactaagag taaaaatgat gcagcattat atggctgttt
    14881 acaatgatag ttgaaagaag gagtcattct aaaatagaat ttgaaaccgt tacgagagat
    14941 gagagctgtt gttagttcca cacatcacac tctatctagg accaatctaa actatatcaa
    15001 ccaacagtgt gccacgggca tattaaattg aagacggttg aacgtataga tctcaaaaat
    15061 gtaaaaaagt atttggtatt gaaattaaaa aagcacctag caactcgttg ggacaatcac
    15121 gatgattgtc tacagttgca ggtggatttg aatatactac tagttatttg ttgtctagga
    15181 taatagattt agtatgttga taagtttgac tcagattcgt attttctaat aaatgataac
    15241 tcacgatatc gattaaaaag agtgtcgcaa tttgtgtgtt gataaattga tggtcggtat
    15301 tacgcgattg atccgttgtt aaaagtacta aatctgcaca atctgtaagt ttactacctt
    15361 caaaatttgt gatggcaacg acatatgcac catgagattt ggcgacttcc gctgcagaaa
    15421 ttaattccga agtattacca ctatttgaca tagcaataaa catatccgaa tgagatagta
    15481 gggatgccga tattttcatt aaatgtgaat cggtagtaac attacctttt agccccatac
    15541 gaatcatacg ataataaaat tcagtcgctg ataaaccaga gctacctagt ccagcaaaga
    15601 gtatatgtcg acttgattga agtttgtcga taaaggtttg gataatgtcg ttatcaataa
    15661 attcaccagt ttgttgaatg atttgttgat gatatttatg aattctttga ataattgggc
    15721 tattttcaat aactgtctct gtcatttctt gttgaatatt aaattttaaa tcttggaaat
    15781 tctcataatc cagcttatga ctaaagcgtg tcatcgttgc tggtgatgta ccaatcgcat
    15841 gggctaagga gttaatcgtt gaaaaggcat cgctataacc attttgtctt atataattga
    15901 cgatgcgttt atcagttttt gtaaataaat gttgataacg ttgaacacga ttctcaaatt
    15961 tcattgtgtc accccttcat cttaatgatt actattatat atgaaaaata ttttcaagat
    16021 agtaaaaagc attgataaaa attatcttaa tgatatattg taaatgactt tacgtgaaaa
    16081 aatgacttat ggagtgagga ataatgttac cacatggatt aatagtatct tgtcaggcac
    16141 taccagatga accattgcat tcatctttta ttatgtcgaa aatggcatta gctgcgtatg
    16201 aaggtggtgc tgttggtatt cgcgcaaata ctaaggaaga cattttagca attaaagaaa
    16261 cggtagattt accagttatt ggcattgtga aacgtgacta tgatcactca gatgttttca
    16321 ttactgcaac gtcaaaagaa gttgatgaac tgatagaaag ccaatgtgaa gtcattgcat
    16381 tggatgcaac gttacagcaa cgtccgaaag aaacgttaga cgaattagta tcatatatta
    16441 gaacacatgc accgaacgtt gaaatcatgg ctgatatcgc gaccgttgaa gaagctaaaa
    16501 atgccgcacg acttggcttt gattatattg gcacgacgtt acatggctat actagttata
    16561 cgcaaggaca attactttat caaaatgact tccaatttct aaaagatgta ctacaaagtg
    16621 ttgatgcaaa agttattgcg gaaggtaatg tcattacacc ggatatgtat aaacgcgtga
    16681 tggacttagg cgttcattgt tcagtcgttg gtggtgcgat aacacgacca aaagaaatta
    16741 cgaaacgttt tgttcaagtt atggaagatt aaatgataac gataaaaaaa cgagatgact
    16801 atcattaatt aaaggtacct aattatctta ggtggctgaa tgaatgtaat gggttcatct
    16861 cgttttgttt gtttatgata gtgattttat tttcaacttt atccaaaaat aagtaaagcg
    16921 acgggaatgg tgattaatag cgacaatgcc acgcgtaaaa accaaatgat gatgagtttc
    16981 cagacaggta ttttaatttc agttgctagt atacatggca ctaatgctga gaaaaagata
    17041 atggctgata cgcttactac accgacgaca aatttagtac tcattgcagc tttagttact
    17101 aacaaagatg gtaaaaacat ttctacaata gaaatcgctg aagcttttgc aagtacagcc
    17161 tgatcagcaa ttgggaaaat ataaataaat ggatagaata tataaccaag ccaatcaatg
    17221 aatggtgtat agttcgctac aatcagtcct aaaaaaccaa tcgataatat agaaggcaaa
    17281 ataccaacag tcatttctaa accgtctttc aaattgtccc aaacgttctt cacgagggat
    17341 ggtgttaatg cattttgttt catcgcctct gcatatgcag ttttcagtct gcttccttca
    17401 atagcaactt cttgttctcc ttcttgtccg ttataatatt ctgttgattc attggtgatt
    17461 ggcggtagcc atgcagtaat tgcagtcacg acaaatgtga tgactaaagt tatccaaaag
    17521 tataaattcc aatgcggcat taatcctaaa gttttagcaa cgataatcat aaaagttgct
    17581 gaaactgttg aaaagccagt cgcaataatc gtggcttctc gtttgttgta catcccttgc
    17641 ttatagacac gattagtaat caataatcct aaggaataac tgccgacaaa cgaagccact
    17701 gcatcgacag cggatttacc tggtgtttta aaaattggtc tcataatagg ctccatataa
    17761 acaccgacaa attctaataa gccatagcct actaataaag aaagcgcaat tgcacctact
    17821 ggaattaaga tacttaatgg catcattaat ttttcaaaca aaaacggacc atagttagct
    17881 ttaaatagta ttgatggacc gattttaaat acatacatta taccgatcat tgcacctgca
    17941 actttaaata atgtaatgac caagtttgtg attgaagtca taaaagtacg tctcactatt
    18001 ggtaacgctg taccaattaa aatcataatc agtgcaacat agggcataag tggacctatg
    18061 attgagcgaa tggctagatg aacatgatcg acgaaaatag tgttgttacc attaatcgta
    18121 aaaggaataa agaaacatag tatgcccact aaactataga caaaaaaacg ccatgcactt
    18181 ggttgttgtg cattagaatg atattgattc attaaagcaa cccctttgtt taaatgaata
    18241 cacaaaactg tatgatgcat cttcccctta atgagatgaa tcattatttt aatttagaaa
    18301 aatctgaaaa cttactataa ttgtatagtt tgaattattt tcataccaat acaaattaac
    18361 taattatata tagattgaaa ctatattact taataaaata tttatcttaa atgttgttgt
    18421 gttgattcaa cgccacaact aaaagtgttt ataaattatt tggaaataca catatttgta
    18481 aa
//
LOCUS       DAHHYJ010000042        18356 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000042 DAHHYJ010000000
VERSION     DAHHYJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18356
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.042"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            123..575
                     /gene="argR"
                     /locus_tag="NBK22_001083"
     CDS             123..575
                     /gene="argR"
                     /locus_tag="NBK22_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCD2414279.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            591..2270
                     /gene="recN"
                     /locus_tag="NBK22_001084"
     CDS             591..2270
                     /gene="recN"
                     /locus_tag="NBK22_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCD2414280.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            2421..3842
                     /gene="lpdA"
                     /locus_tag="NBK22_001085"
     CDS             2421..3842
                     /gene="lpdA"
                     /locus_tag="NBK22_001085"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD2414281.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQFGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSTSGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDANEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            3858..4850
                     /locus_tag="NBK22_001086"
     CDS             3858..4850
                     /locus_tag="NBK22_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCD2414282.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            4850..5833
                     /locus_tag="NBK22_001087"
     CDS             4850..5833
                     /locus_tag="NBK22_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD2414283.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            5846..7120
                     /locus_tag="NBK22_001088"
     CDS             5846..7120
                     /locus_tag="NBK22_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD2414284.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQAKVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTKSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            7471..7908
                     /locus_tag="NBK22_001089"
     CDS             7471..7908
                     /locus_tag="NBK22_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCD2414285.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            7922..8902
                     /locus_tag="NBK22_001090"
     CDS             7922..8902
                     /locus_tag="NBK22_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD2414286.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDAHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDSIAIEFEALLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVAALLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(9029..9208)
                     /gene="prli42"
                     /locus_tag="NBK22_001091"
     CDS             complement(9029..9208)
                     /gene="prli42"
                     /locus_tag="NBK22_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCD2414287.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            9471..10604
                     /locus_tag="NBK22_001092"
     CDS             9471..10604
                     /locus_tag="NBK22_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCD2414288.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFISLGLDVKE
                     DQAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQISHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIIGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAEVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            10672..12078
                     /gene="gndA"
                     /locus_tag="NBK22_001093"
     CDS             10672..12078
                     /gene="gndA"
                     /locus_tag="NBK22_001093"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCD2414289.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            12297..12668
                     /locus_tag="NBK22_001094"
     CDS             12297..12668
                     /locus_tag="NBK22_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2414290.1"
                     /translation="MEKLNQVMLYVDDVDKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRVFNFQDGQGNYFAVSD"
     gene            12607..12861
                     /locus_tag="NBK22_001095"
     CDS             12607..12861
                     /locus_tag="NBK22_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414291.1"
                     /translation="MVNVYLIFKMAKVTILQLVTKQSILIFIQTFILIFRLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYSGWKRFPMQKLTGGD"
     gene            12862..13881
                     /locus_tag="NBK22_001096"
     CDS             12862..13881
                     /locus_tag="NBK22_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2414292.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDENMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGDFEVSKDRIQGFETIASQNKINYQI
                     IETSNEREVILNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTCIDIKPRMLGQQAGSAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            13897..15546
                     /locus_tag="NBK22_001097"
     CDS             13897..15546
                     /locus_tag="NBK22_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCD2414293.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDRSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWGNPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     DKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHATVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEAFVIELN"
     gene            complement(15628..16494)
                     /locus_tag="NBK22_001098"
     CDS             complement(15628..16494)
                     /locus_tag="NBK22_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD2414294.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(16724..18208)
                     /locus_tag="NBK22_001099"
     CDS             complement(16724..18208)
                     /locus_tag="NBK22_001099"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCD2414295.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
BASE COUNT         6568 a         2594 c         3451 g         5743 t
ORIGIN      
        1 taaatagata accaagatag ataaaccatg tttacccttt ttacgtaaag gtgtttagta
       61 gtcgtaaatc agtgcatgtg ttaaaataaa tgtataaata ttcgtaatca gaggtgttta
      121 ctgtgcccaa aaaatcggtt aggcatataa aaattagaga aattatttca aatgaacaga
      181 tagagacaca agatgaatta gttaaacgat taaacgatta tgatttaaat gtcactcaag
      241 caactgtttc tcgtgatatt aaagaactac aacttattaa agtacctata ccttcaggtc
      301 aatatgttta tagtttacca aatgatagaa aattccatcc tttagaaaaa ttgggacgtt
      361 atttaatgga ttcctttgtt aatatagatg gtactgataa tttacttgtt ctaaaaacat
      421 tacctggtaa tgcacaatct attggagcta tattagacca aatcaattgg gaagaagtac
      481 taggcacaat ttgtggtgat gatacttgtt taattatttg tcgaagcaaa gaggcaagtg
      541 atgaaatcaa gtcaagaatt ttcaatttgt tataaggatg cgatgagttt atgttacaaa
      601 ccttatcaat caagcaattt gctattattg aagagttaga aattcaattt tctgatggtt
      661 taactgtttt aagtggtgaa acgggttcag gtaagtcaat tattatagat gcaataggac
      721 aattaattgg aatgagagct tcatctgatt ttgtaagaca tggtgaaaaa aaggctgtca
      781 tcgaaggtat atttgatatt gacgagagta aagatgctat acatatattg aaaaatatgg
      841 atattgatgt agatgaagat tttttattag ttaaacggga aattttcagc tctggtaaaa
      901 gtctttgtaa aattaataat caaacagtta ctttacaaga tttacgaaaa gttatgcaag
      961 aattattaga tattcatggc caacatgaaa cgcagtcttt gttgaaacaa aaataccatt
     1021 taacattatt ggataattac gcagaatcac gttatcaaga tttgttggat aaatatcatc
     1081 aaacttttca aaattataaa gccaaaaagc aagagttaga agatttagaa tcagcagacc
     1141 aagcattgct gcagcgttta gatttaatga aattccaatt agaagaattg tctgaagcac
     1201 atctaaaaga aggcgaaata gagcaacttg aaatagatat taaacgcatt caaaactctg
     1261 aaaaattaag cttagcgctt aataacgcac atatgacatt aacggatgaa aatgctatca
     1321 ctgatcgttt gtatgaacta agcaaccatt tgttaacaat aaacgatata gttccaaaca
     1381 aatatgacaa attaaaagaa gatattgatc aattttacta cattttagaa gatgcaaaac
     1441 atgagttata tgatgaaatg gctaatacag aattcgacga acaagtgtta aacgagtatg
     1501 aatctcgtat gaatttgctg aataatttaa aacgtaaata tggaaaagat atttcagaat
     1561 taatcgcata tcaagaaaag cttaataatg aaatcaataa aattgaaaac tatgaacaaa
     1621 gtacatcgca gttacgagaa gaaataaatg cattgtataa tcaagttata gaggttggac
     1681 aagcgttatc aaagcagcgt cgtatagtcg ctagggagtt aagagaccac attgtatcgg
     1741 aaattcaaaa cttacaaatg aaagacgcaa atcttgagat ttcatttaaa aaattagaag
     1801 aaccgaatat tgatggaatc gaatttgtag aatttttaat cagtccaaat aaaggggaac
     1861 cattaaaaag tttaaataaa attgcgtcag gtggagaact ttctagaatt atgttagcgt
     1921 taaaaagtat ttttgttaaa tcgagaggtc aaactgcaat tttattcgat gaggttgact
     1981 caggtgtatc tggacaagcc gcacaaaaaa tggcagagaa aatgcgagat atagcagaat
     2041 atatacaagt tatttgtatt tctcacttgc cacaagttgc atcgatgagc gaccatcatt
     2101 tattaatttc gaagtcatca aaagatgatc gtacaacaac acaagttcaa gagttaattg
     2161 gcgatgataa agtagatgaa attgcacgaa tgatttcagg tgcaagtgtt actgatttaa
     2221 ctcgagaaaa tgcaagagaa atgatacaac acaatcaaag acgtagataa tttccaaagt
     2281 gtgttgaata ctcatttatg gatttattta tgtgcattca aactaaatag acaatcatgt
     2341 aaaataggta attaatggac atgaaaacta tgtattaata aatttttaat ggatatatat
     2401 cattagttag gagtaagaac atgtcagaga aacaatatga tttagtcgtt ctcggtggag
     2461 gtactgcagg atatgttgct gcgatacgtg cgtctcaatt tggtaaaaaa gtggcaatag
     2521 tagaacgaca actattagga ggtacttgtc tccataaagg ttgtattcct actaaatcgc
     2581 tgcttaaatc tgctgaagta tttcaaacag taaaacaggc agcaatgttt ggcgttgatg
     2641 tcaaagatgc taatgttaat tttgaaaata tgttggcacg aaaagaagac attattaatc
     2701 aaatgtatca aggtgtaaag catttaatgc aacacaatca cattgacatt tataatggca
     2761 cgggacgtat tctaggtaca tctatatttt cacctcaaag tggcacaatt tctgtcgaat
     2821 atgaagatgg tgaatcagac ttattaccaa accaatttgt attaattgca actggttctt
     2881 cgcctgcaga gttgccattc ttatcatttg atcatgataa aattttatct agcgatgata
     2941 tattatcatt aaagacgtta ccatcaagta ttggtattat tggtggtggt gttatcggaa
     3001 tggaatttgc atcattgatg atagatttag gtgttgacgt aacagttata gaagcaggtg
     3061 aaagaatttt accaactgaa agtaaacaag cttcacaact attaaaaaaa tcattgtcag
     3121 cacgtggtgt taaattctat gagggaataa aactttctga aaatgatata aatgttaacg
     3181 aagatggtgt tacgtttgaa atttcctcag acataattaa agtagataaa gtgttgctat
     3241 cgattggtag aaaacctaac acatcagata taggtttaaa caacactaaa ataaaacttt
     3301 caacatcagg acatatttta acgaacgaat ttcaacaaac tgaagataaa catatttatg
     3361 cagctggtga ttgtattgga aaattacaat tggcacatgt tggatcaaaa gaaggtgttg
     3421 tggctgtcga tcatatgttt gaagggaacc caatcccagt aaactataac atgatgccta
     3481 agtgtattta ttcacaacct gaaattgctt ctatcggttt aaacattgag caggcaaagg
     3541 cagagggaat gaaagttaaa agttttaaag taccatttaa agcaattggt aaagcagtga
     3601 ttgatagcca tgacgcaaac gaagggtata gcgaaatggt gattgatcaa tcaactgaag
     3661 aaattgtggg tattaatatg attggtccac atgtaacaga attgattaat gaggcatcac
     3721 tgttacagtt catgaatggc tcggcattag aattaggact aacaacacac gcacatcctt
     3781 ccatctctga agtgttgatg gaattaggat tgaaagcaga aagtagagct attcacgtat
     3841 aaataggagg atttaacatg attgattata aatcattagg ccttagcgaa gaagacctaa
     3901 aagtaatata taaatggatg gatttaggta gaaaaataga tgaaagactg tggttattga
     3961 accgtgcagg taaaattcca tttgttgtaa gtggtcaagg acaagaagca actcagattg
     4021 ggatggcata cgcattagaa gaaggtgata ttacagcgcc ttattataga gatctagctt
     4081 tcgttactta tatgggtatc tcagcgtatg atacgttttt atcagcgttt ggaaagaaag
     4141 acgatgttaa ctctggtgga aaacaaatgc catcacactt tagtagtaga gctaagaata
     4201 ttttgtctca aagttcaccg gtagcaactc aaatacccca tgctgttggt gctgctttag
     4261 ctttaaaaat ggatggtaag aagaaaattg caacagcaac tgttggtgaa ggtagctcaa
     4321 accaaggtga cttccatgaa ggtttaaact tcgcgggtgt acacaaactt ccttttgtat
     4381 gtgtcattat aaataataaa tatgcaattt cagtacctga ttcattacaa tacgcagctg
     4441 aaaaattatc ggatagagca cttggttacg gtatacatgg tgaacaagta gatggtaatg
     4501 atccattagc aatgtataaa gcgatgaaag aagcaagaga tcgagctatt tctggtcaag
     4561 gttcaacttt gattgaagca gtaacaagtc gtatgacagc tcattcatca gatgatgacg
     4621 accaatatcg tacgaaagaa gagcgtgaag cgcttaaaaa agcagactgc aatgaaaagt
     4681 tcaaaaaaga gttgctttca gctggcatta tcgatgatgc ttggttggca gaaatagaag
     4741 cggagcataa agacatcatc aataaagcga caaaggctgc tgaagacgcg ccatatccaa
     4801 gtgtcgaaga agcctatgca tttgtgtacg aagaagggag tcttaactaa tggctaaatt
     4861 atcttattta gaggcgatac gccaggcaca agacttagca ttacaacaaa ataaagatgt
     4921 gtttattctt ggtgaagatg ttggtagaaa aggtggcgta tttggtacta ctcaaggact
     4981 gcaacaaaaa tatggtgaag acagagttat cgatacgcca ttagctgagt caaatattgt
     5041 tggaactgcc attggtgcag ctatggtagg taaaagacca attgcagaaa ttcaatttgc
     5101 agattttatt ttacctgcga caaatcaaat tattagtgaa gctgcgaaaa tgcgttatcg
     5161 ttcaaataat gattggcaat gcccattaac gattcgtgca ccatttggtg gcggtgttca
     5221 cggaggacta tatcattcac aaagtattga aagtatattt gcttcatcgc caggtttaac
     5281 aattgttatt ccttccacac cgtatgatgc taaagggctt ttattatcat ctatcgaatc
     5341 aaatgatcca gtattgtact ttgagcataa aaaagcttat cgtttcttaa aggaagaagt
     5401 gccagaagaa tattacactg tacctttagg aaaagcagat gtgaaacgtg aaggtgaaga
     5461 tttaacagta ttttgttatg gattaatggt caattattgt ttacaagcag cggatatttt
     5521 agctgcagac ggtatcaatg ttgaagtcgt tgatttacga actgtttatc cattagataa
     5581 agaaacaatt attgatcgtg ctaaacatac aggtaaagtt ttacttgtaa ctgaagataa
     5641 tttagaaggt agtattatgt cagaagtgtc agcgattatt gcagagcatt gcttgttcga
     5701 tttagatgca ccaatcatgc gtttagctgc tccagatgta ccatctatgc cattttctcc
     5761 tgtattagaa aatgaaatta tgatgaatcc agaaaaaatc ttaaataaaa tgcgtgaatt
     5821 agcagaattc tagggaggga aagtcatgga aataacaatg cctaagttag gtgagagtgt
     5881 tcatgaaggc accattgaac aatggttagt ttctgttggt gatcatattg atgaatatga
     5941 accattatgt gaagttatta cagataaagt gacagctgaa gtcccttcca cgatatcagg
     6001 aacaattaca gaaattttag ttgaagcggg gcagacagta gctattgata caattatctg
     6061 taaaattgaa actgctgatg aaaagacaaa tgaaacaact gaagagatac aagcaaaagt
     6121 ggatgagcat actcagaaat ctactaaaaa agctagtgca acagtggaac agacatctac
     6181 tgctaaacaa aatcaaccac gtaataatgg tcgcttttca cctgttgtat ttaaactcgc
     6241 ttcagagcat gacattgatt tatcacaagt tgtaggtagt ggatttgaag gtcgtgtaac
     6301 taagaaggat ataatgtcag ttattgaaaa tggtggtacc acagctcaat ctgacaaaca
     6361 agttcaaaca aaatcaacat cagtagatac atcaagtaac caatcatctg aagacaatag
     6421 tgaaaacagc acaataccag taaatggtgt gcgtaaagca attgcgcaaa atatggttaa
     6481 tagtgtaaca gagattccac atgcatggat gatgattgaa gtagatgcta caaatcttgt
     6541 gaatacgaga aatcattata aaaacagctt taaaaataaa gaaggatata atctaacgtt
     6601 ctttgctttc tttgtaaaag ctgtagcaga tgctttaaaa gcatatcctt tattaaatag
     6661 tagctggcaa ggaaatgaaa ttgtcttaca taaagacatt aatatttcaa ttgctgttgc
     6721 tgatgaaaat aaattatacg tacctgtgat taagcatgca gacgaaaagt caatcaaagg
     6781 tatagctaga gaaattaata ctttagcaac gaaagcgcgt aataagcaat tgacagctga
     6841 agatatgcag ggcggtacat ttacggtaaa taatactggt acatttggtt cagtatcatc
     6901 aatgggtatt ataaatcatc ctcaagcagc gattttacaa gtagaatcaa tcgttaaaaa
     6961 gccagtagta attaatgata tgattgcaat tcgtaacatg gttaatttat gtatttcaat
     7021 tgatcatcgt attttagatg gtttacaaac aggtaaattt atgaatcata ttaaacagcg
     7081 tatcgaacag tatactttag aaaatacaaa tatatattag tgataacata gatgcatcta
     7141 tcgacaactt gttttatctt gttcttgtcg atggatgtat tattttttgg cactaaaata
     7201 tgtgcaatat attcaaaaaa gataaagaac aataatcaac atggttgatt gaatttttgc
     7261 agtaaagtca aaataagaca tcatacttga aacatattaa tgaaaacatg tgaacaaatt
     7321 agttaccatg attttaagca caataatgtt tggtatattg ttaaaattgt gtctaaatat
     7381 aggtgtgatt cagattagtt tattgaacaa tatgttatta attagtagaa tgaggatagt
     7441 ttaaatataa agggataggt gattgaactt atggacatga atttcgattt atacatgaac
     7501 ggtgttgtag aacaagcaag gaatgaaatt gaatctgcgg gatatgagca attaactact
     7561 gcagaagatg ttgacaaagt tcttaaacaa gatggtacaa cactagttat gatcaattct
     7621 gtatgtggtt gtgcaggtgg tatcgcaaga ccagcagcat cacatgcttt acattatgac
     7681 gtattacctg atcgtctagt gacagtattt gctggacaag ataaagaagc gacacaaaga
     7741 gcgcgtgaat acttcgaagg ttatgcgcct tcaagtccgt catttgcatt agtaaaagat
     7801 ggaaagatta cagaaatgat tgaaagacat caaatcgaag gtcatgatgt gatgaacgta
     7861 attaatcaat tacaaacatt attcaataaa tattgtgaag aaagataaga ggcgctaacc
     7921 catgttaaag ttaaatcctt acaagattgg atttagaaca ataaaaacag cagtgggtat
     7981 gactttaggt gtaattatta gtaagctgtt aggtttagat aattatgctt caagcgccat
     8041 attagtcgta ttatgtatta aacatacaaa agtacattcg ctacaagcga ttatttcaag
     8101 attagtatca tgttttttag tattgttttt aggttcagca atatttagtt tattaggtca
     8161 gagtccaatt gtactcggta ttatcgtatt gttatttata ccattaactg tcgtattaaa
     8221 agtacaagaa ggtgtcatta cgagttgcgt tatattactt catgttttta atgcaaaatc
     8281 aattgatgca catttaattg ttaatgaaac attattactg ttaattggac tgagcattgc
     8341 atttacaatg aatttaatga tgccaagttt agacaaacaa ctagacgaat acaaatgtaa
     8401 aattgagcaa caaattgctg atatttttag taaatatagt tatatttgtg aaaaatatga
     8461 agatagcatt gcgattgaat ttgaagcgtt acttttaaat attaaaaagg cgaagtctat
     8521 cgcgttccga gatgttaaaa atcattttgt tagaaacgaa aattcatact atcattattt
     8581 tgatatgcga gaagagcaag tggaattgtt aatgagaatg aaaccgctca tcgaaagtat
     8641 ctgtcataaa gatccacttt taaatgatgt tgcggcattg ctgaaagaaa ttggtctaaa
     8701 tgtgaatagt aatgactata cagcaatgcg tttacacaat ttgtatgaga cgcgtttgaa
     8761 attggatgaa ctgccattgc caacaacaca caaagcgctt aactcgagag caagtattat
     8821 tcaaatatta aatgaattag aagaatattt acaaatcaaa tcgcaatttg gttcattgaa
     8881 aatgcatagt gaagtagctt aaaaaataat ccatccgtat gacccatgtt tgaaagtaat
     8941 cgttgcaata cgataactta ttaatacatg cgtttataat ggatggattt ttaattgtat
     9001 gattttgtat aggtagttga gatatagttt ataaaattgg aatgatactc gttaataaca
     9061 atgctaatac aactgataca agcataacga taatgataat tcttaaaact ttattttgca
     9121 ttttgtgtgc ctccaaatga ataacctttg tattaataat tacgacataa gatataaaac
     9181 ttttaacttg agacgatata actagcaatc atcataaagg aatataataa aaaacatgag
     9241 taagtttgct tatgtcattt tcctcattat acgatatctt tgaaaaatca atcaatatat
     9301 ttttgacaaa gcttagtttg agttacaata aatatatata ttaaattgaa aataatatga
     9361 cacaatttac aagcctgata aatctgtgta acgtcaatta aagtaatatt ttatttcatc
     9421 aatttttacc gtttggcgta cttgaaaatt ttgataaaag gggttccatt atgattaatg
     9481 agcaaagatt acttaatacg tttttagaac ttgtacaaat cgattctgag acagggaatg
     9541 aatcaacaat ccaacctatt ttaaaagaaa aattcatatc attagggtta gatgttaaag
     9601 aagatcaagc ggctaaacat cctaaattag gtgccaataa tctagtgtgt acaatgaata
     9661 gtactatcga agaaggcgaa gtacctaaat tatatttgac gagccatatg gatactgttg
     9721 tacccgcaat taatgtaaag ccaattgtaa aagatgatgg ctacatatac tctgatggta
     9781 cgactattct aggtgcagat gataaagcag gattagcagc gatgcttgaa gttttgcaag
     9841 tgataaagga acaacaaatc tcacatggac aaattcaatt tgtgattact gttggagaag
     9901 aatcaggttt aattggtgct aaagaattaa attcagagtt gttggacgcg gactttggtt
     9961 atgctattga tgcaagtgct gatgtcggca ctacggttgt aggtgcaccg acgcaaatgt
    10021 taatttcagc taaaattatt ggcaaaacgg ctcatgcaag tacgccaaaa gagggtgtta
    10081 gtgctattaa tattgcggct aaagcaatta gtcgaatgaa attaggacaa gttgatgaaa
    10141 taactactgc caatattggt aagtttcatg gcggttcagc tacgaatatt gttgcagatg
    10201 aagtcatttt agaagcagag gcacgttctc acgatccaga aagaataaaa acacaagtca
    10261 aacatatgac tgatgtgttt gaaactactg catcagaatt aggtgggaaa gcagaagtta
    10321 cagtggagca aagttatcca ggatttaaaa ttaatgataa tgaagcggtt gttaaaattg
    10381 cacaagaaag tgctcgtaat ttaggattat ctgcaaatac aattatttct ggtggcggtt
    10441 cagacggcag tattattaat acatttggta ttccttcagt cattttagga gtgggttatg
    10501 aaaaaatcca tacaacaaat gagagaatgc cgattaaatc attgaacttg cttgcaagtc
    10561 aagtgttaga aataatcaaa atagttgcgc gacaatcgaa atgatttagt gtagtgtatg
    10621 tctattttgt gatacaatac taatgaaaaa tcaacaagtg aggtaagtaa tatgacacaa
    10681 caaattggag ttattggttt agccgttatg ggtaaaaacc tagcttggaa tattgaatca
    10741 cgcggatata gtgtatctgt atttaaccgc tcaagtgaaa aaactgattt aatggttgaa
    10801 gaatcaaaag ggaaaaatat tcatccaacg tactcattgg aagaatttgt taattcttta
    10861 gaaaaaccac gtaaaatttt attaatggtt caagcaggta aagctacgga tgcaacgatt
    10921 gatagtttgt tacctttatt agatgatgga gatattttaa ttgatggtgg taatacaaac
    10981 tatcaagata ctatcagacg taataaagca ttagcacaaa gtgcaatcaa ctttattggt
    11041 atgggcgttt ctggtggtga aattggagcg ttaactggtc cgtcattaat gccgggtgga
    11101 caagaagaag cttataacaa agttgcagat attcttgatg caattgctgc aaaggcaaaa
    11161 gacggtgctt catgtgttac ttatattgga cctaatggtg ctggacacta tgtgaaaatg
    11221 gtacacaatg gtatcgaata tgctgacatg caattaattg cagaaagtta tgctatgatg
    11281 aaagaattat taggcatgtc tcatgaagac attgctcaaa cgttcaaaga ctggaatgct
    11341 ggtgaattag aaagttattt aattgaaatc actggtgata ttttcatgaa attagatgaa
    11401 aataaagaag cattagttga aaaaattctt gatactgcag gtcaaaaagg tacaggaaaa
    11461 tggacttcaa ttaacgcatt agaattaggt attccattaa caatcattac tgaatctgta
    11521 tttgcacgtt ttatctcttc aatcaaagaa gagcgcgtaa atgcttctaa agaattaaat
    11581 ggtccaaaag catcatttga tggtgacaag aaagactttt tagaaaaaat tcgtaaagca
    11641 ttatatatga gtaaaatttg ttcttatgca caaggtttcg cgcaaatgcg taaagcaagt
    11701 gaagataatg aatggaattt aaaacttggt gatttagcta tgatttggag agaaggttgc
    11761 attatccgtg cacaattctt acaaaagatc aaagatgctt acgataataa tccaggatta
    11821 caaaacttat tgctagatcc ttactttaaa aacatcgtta ctgaatatca agatgcgtta
    11881 cgtgatgttg tcgcaactgg tgttcaaaat ggtgtaccaa cacctggttt ctcatcaagt
    11941 attaattact atgatagtta ccgtgcagct gatttacctg caaacttaat tcaagcacaa
    12001 cgtgactact tcggtgctca cacgtacgaa agaaaagata aagaaggcgt attccataca
    12061 caatggattg aagaataata ttgattaaag gatagttgaa agatactatc cttttttata
    12121 tcttttgata aatataaaag agagtgtgat agagtagcta caacctataa aaaattacac
    12181 aagtattatc tttgacctta aaagcatccc gccatatttt taagcatttt gtgaagtaat
    12241 aaaatattta ctgtgttatg atagcatgta ctatagagaa atagaggtat taacttatgg
    12301 aaaagttaaa tcaagttatg ttatatgttg atgatgttga caaagcgaaa gcattttgga
    12361 cagaaacttt agaatttgtt gttgtaagtg aaacaccgtt agctgaggac tatgttgcag
    12421 ttgaagtatc acctacgaaa gatgctgaaa cgtcattgac gattatggca aaagaattta
    12481 ttgaaaagta tagcccggaa gtgaacctag gaacaccatc tttaatgttt aaagagaaaa
    12541 actttgatgc attatactca aaattaaatg atttaggttt aactgggcat gatatagtag
    12601 aaatgaatgg tcaacgtgta tttaattttc aagatggcca aggtaactat tttgcagtta
    12661 gtgactaaac aatcgatttt aatatttata caaaccttta tattgatttt ccgattgaat
    12721 ttcagtatga aggttttatt tttatcgttt tatgcaatcg tttgcacaaa tcttttgact
    12781 tttttagaaa gtgtgactaa actggtatta tacagtggtt ggaagcgttt tccgatgcag
    12841 aaattaacag gaggtgatta aatggttacg attaaagatg ttgcactaaa agccggtgtt
    12901 tctccttcga cagtttcaag agttataaaa ggaaatcaac gtattagcga agcgacaatt
    12961 tcaaaagtga agaaagttat ggaagaattg aattattttc ctaataccgc tgctagaact
    13021 ttaattacaa accaaacata taaaattggt ttagtgttaa aagggtctga ggagcctatt
    13081 cgactgaatc cattctacat caatgtattg ctagggattt ctgaaacgtg caaccagcat
    13141 ggctatggta cacaaacgac agtctcaaat aatatgaatg atttaatgga tgaagtttat
    13201 aaaatgatta aacaacgaat ggttgatgcg tttatactgc tctattcaaa agaaaatgat
    13261 ccgattaaac aaatgttaat tgatgaaaac atgccattta ttgtgattgg taagcctaca
    13321 tcggatatag atcatcaatt tacacacata gataatgata atatattagc ttctgaaaat
    13381 ttgacacgac atgttattga acaaggtgta gatgaattaa tatttattac agaaaaaggc
    13441 gattttgaag tttcgaaaga caggattcaa ggatttgaaa cgatagcttc tcaaaataaa
    13501 attaattatc aaattattga gactagtaat gaaagagaag ttattttaaa ttacatgcaa
    13561 aatctacata cgcgtttgaa ggacccaaat attaaacagg caatcatttc gttagatgct
    13621 atgttacatt tagcgatttt aagtgtccta tatgaactta atattgaaat tccgaaagat
    13681 gtaatgacag caacgttcaa tgattcttat ttaaccgaaa ttgcgtcacc acctcaaacc
    13741 tgtattgaca tcaagcctcg aatgttaggt caacaggctg gttcagcaat tttaaatata
    13801 ttgaaaaaca aagcacagga tgttattgaa ctcgtcatta tagatacaga attaaaaata
    13861 agaaaatcaa cacagcgata gcaaaggagt tttaaaatga ataagcaatg gtggaaagaa
    13921 gcagtagcat atcaagtata tccaagaagt tttaatgata gtaatcacga tggtattggg
    13981 gatttacctg gaatgattga taaattggac tacttaaaag atttaggtat cgatgtcatt
    14041 tggctcagtc caatgtttaa atcacctaat gatgacaatg gttatgatat tagtgactac
    14101 caagagatta tggatgaatt tggaacgatg gaagactttg atcgtttatt aaaaggtgtt
    14161 catgatagag gcatgaagct tattttagat ttagttgtaa atcatacatc tgatgaacat
    14221 ccttggttta tagaatctaa atctagtaaa gacaatccca aacgtgattg gtatatttgg
    14281 caagatccaa agccagatag gtctgaacct aacaactggg aaagtatctt taatggatct
    14341 acatgggaat atgatgctaa tactgagcaa tattatttcc atctattcag taaaaaacaa
    14401 cctgatttga actggggtaa tccggaagtt agagatgctg tatttgaaat gatgaactgg
    14461 tggtttgata aaggcattga tggatttaga gtagatgcaa ttacgcatat taagaagacg
    14521 tttgaagcgg gtgacttacc tgtacctgag gataaaacat atgccccagc atttgatgta
    14581 gatatgaatc agccaggtat acaaacttgg ttacaagaga tgaaagatcg ctcattaagt
    14641 aagtatgaca ttatgactgt tggtgaagcg aatggtgtaa gccctgatga tgctgatgac
    14701 tgggtcgggg aagaaaatgg taaatttaat atgatattcc aatttgaaca tttgggactg
    14761 tggaatagtg gtgattctca ctttgatgta aattcgtata aatctgtatt aaatagatgg
    14821 caaaaacaac ttgaaaataa aggttggaat gcgttgttta ttgaaaatca tgaccaacca
    14881 cgacgtgtat cgacgtgggg tgacgatgac aagtattggt atgaatcagc aacaagtcat
    14941 gcaacagttt atttcttgca acaaggtacg ccattcattt atcaaggtca agaaattggt
    15001 atgacgaatt atccatttga aagtattgaa acgtttaacg atgttgctgt taaaaatgac
    15061 tatcaaatag tgaaagctca aggtggagat gttgacgctt tacttgcgaa atataaagat
    15121 gagaaccgag ataattctcg cacaccaatg caatgggatg atacgttaaa tggaggattt
    15181 acaaatggtg aaccgtggtt cccagtgaat ccgaattata aaactatcaa tgttgcacaa
    15241 caattagaag atgagcattc agtattacaa ttttataaag atttaattca attaagaaag
    15301 tctaatgatg tatacgtata tggtcaattt gatttagtag atgctgaaaa ttcacaagtt
    15361 tttgcataca cgagaacatt aaatgaaaag caagttctta ttgtaggtaa tcttactaac
    15421 cacgaagcag aattaactgt accatttgat ttaagccatg gagaagtaaa gctatttaat
    15481 tatgatgcca aagttaattt aaaacagtta cgtccatatg aagcatttgt tatcgaacta
    15541 aattaacata ttaatatgcc ccctaataga atctaaggct atttgtagac ctatagatat
    15601 attagggggc ttctttgttt ttaattttta atcttcgtca taaggactct gtttaacagg
    15661 aatccaaagc tgaattttag tgaatgggtc attaaatgaa atatcgagag ggtacacttc
    15721 aacatataaa tcatttcgtt catatggcaa tgttaactgc aaacttgatt caatatagta
    15781 ccaagcttca ttagttgcat aatcaatttc accttgtaaa ttgaatttcg cacaatgttt
    15841 gccaggtaaa aatcgacttt ctaagtgtgc aggataacgt tcacttggta cacctacaaa
    15901 tatttctaaa ccattttcaa gaggacaact aataacaaat agttcaaatg gactaacgtc
    15961 attatatcgt cgtaactctt taattttacc atcaatgagc aagtcttcta aaaaatccgg
    16021 aacattaaaa ggatgtgaca aatacttagt gtctataaat cgtgcatatc caaccaatga
    16081 aatatcatct gtctcttcta atctgtatgg atagggtgct ctctcagtag ttgataattt
    16141 gatatataat cgctcttgaa tttgtaattc atctttttta gtagaagctt gaattggtga
    16201 tacgccgtga aaatcactaa aatcatttgc aaaatcattt gaatttgcat agtgatattt
    16261 tttagcgata tctacaagtc gtgtagcacc attaatcaca tcatttgcag cgagtgtcat
    16321 tttacgcgcg cgtgcatatg cttctggaga taagccgaca atcattttaa atgattgatc
    16381 aagatggtat ggcgaaagac caacgtaatc acttaattct tgcaaattga aaggctctaa
    16441 taaacgatct tcaatataaa caattgcctg ttgtatttgc ttgataacgt ccaaaacttt
    16501 cactccaatt aattcaatca tttattttta ttctacatta tttctataaa ttatacaccc
    16561 atttgttcaa tgattattaa aatagttttg ggcattgtaa aatataatta gataatatag
    16621 tctagaaaaa aagcgaatga tagaacaatt gatttacttg attcgtaatc aatccttgtc
    16681 attcgctcat ttatttttgt ttaacatgtg cgttttaatt caattattga atatcgtccc
    16741 accaatggtt accatcacga gcaagtaata aatcactttc taatggacca ttagtacctg
    16801 attcatagtt agggaattct ggatcaacca tattccattc atcttgaatt gcatcaacaa
    16861 atttccatgt agattttaat tcttcccagt gcgtaaagtt agtggcatca cctttaagac
    16921 aatcgaataa tagattttca tatgcatcta cagtattcat tttatcttga gcgctcattg
    16981 aataagacaa ttgaacaggt tcagtttcga taccttgtgt atttttctta gcatttagat
    17041 gtaaagatac accttcatta ggttggatat tgattactaa taggtttgaa tctaatagtt
    17101 tatcagtttc atagtataag ttcattggta cttctttaaa ttcaacgaca acttgaattg
    17161 ttttagattt catacgttta ccagtacgaa tatagaatgg tacaccagcc catctaaagt
    17221 tatcaattgt taatttacct gaaacaaagg taggtgtgtt agagtcatct gcaacgcgat
    17281 cttcatcacg gtatgcttta acttgtttac catcgatata gccttcgcca tattgaccac
    17341 gaacaaagtt ctttttaaca tcttcagatt ggaaatgacg cagtgattta agtactttta
    17401 ctttctcagc acggatatct tcactattta aactaatagg tgcttccata gctaataatg
    17461 caaccatttg taacatgtgg ttttgcacca tatcttttag cgcgccactt gattcataat
    17521 aaccaccacg atcttcaaca cctagtattt cagaagatgt aacttggatg tttgaaatat
    17581 atttgttatt ccataatggt tcaaacatcg cattcgcaaa acgtaatacc tcgatatttt
    17641 gaaccatgtc ttttcctaaa tagtggtcaa tacgataaat ttcttcttct ttaaatgatt
    17701 tacgaatttg attgtttaat gcttcggctg attttaaatc actaccgaat ggtttttcga
    17761 taacaaggcg tttaaatcct tttgtatcag taagaccaga agattttaga taatcagaaa
    17821 taacgccaaa gaattgtggt gccattgcta aatagaatag tcgattacct tttaattcaa
    17881 attggctatc taattcatta ctaaaatcta gtaattcttg atagctttct tcattactaa
    17941 catcatgtct atgatagaag acatgttcca taaacgcgtc aattttgttt gtatctttta
    18001 cgtgcttttg aattgatgat tttacttgat tacggaaatc atcattagta atgtcacgac
    18061 gtccaatacc gatgatggca atatgttcat ctaaattgtc ttgttggtag agatggaata
    18121 ttgatggaaa caacttacga tggcttaagt caccagttgc accaaagatt gtgattaaac
    18181 aagggatgtg tttgttttta gtactcaaga ttaaaacctc aattcttaat tagatatatg
    18241 atttattatt ataagataat ccattcagca ggtcattcaa tatgctcgac agtatgcagt
    18301 gtcttaaatg aaatatcgat tcatgtatca tgtttaatgt gatacttatt aatgat
//
LOCUS       DAHHYJ010000043        18234 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000043 DAHHYJ010000000
VERSION     DAHHYJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18234
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.043"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            48..2570
                     /gene="mprF"
                     /locus_tag="NBK22_001100"
     CDS             48..2570
                     /gene="mprF"
                     /locus_tag="NBK22_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCD2414296.1"
                     /translation="MNQEVKNKIFSILKITFATALFIFVAITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(2773..3282)
                     /gene="msrA"
                     /locus_tag="NBK22_001101"
     CDS             complement(2773..3282)
                     /gene="msrA"
                     /locus_tag="NBK22_001101"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD2414297.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            3419..4402
                     /locus_tag="NBK22_001102"
     CDS             3419..4402
                     /locus_tag="NBK22_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD2414298.1"
                     /translation="MDKETNDNEYRRQSEYRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(4490..4675)
                     /locus_tag="NBK22_001103"
     CDS             complement(4490..4675)
                     /locus_tag="NBK22_001103"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCD2414299.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            4823..6085
                     /locus_tag="NBK22_001104"
     CDS             4823..6085
                     /locus_tag="NBK22_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCD2414300.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVILAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEDEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(6222..7313)
                     /locus_tag="NBK22_001105"
     CDS             complement(6222..7313)
                     /locus_tag="NBK22_001105"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCD2414301.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSELIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEPRNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RDITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAQA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            7478..8509
                     /locus_tag="NBK22_001106"
     CDS             7478..8509
                     /locus_tag="NBK22_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD2414302.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYRNNHEIPRDQKHMEIRIDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            9001..10407
                     /locus_tag="NBK22_001107"
     CDS             9001..10407
                     /locus_tag="NBK22_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCD2414303.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQITERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVIKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDQPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEITGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVRP"
     gene            10404..10970
                     /locus_tag="NBK22_001108"
     CDS             10404..10970
                     /locus_tag="NBK22_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCD2414304.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTL
                     KVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTEDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            10976..11974
                     /gene="trpD"
                     /locus_tag="NBK22_001109"
     CDS             10976..11974
                     /gene="trpD"
                     /locus_tag="NBK22_001109"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCD2414305.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAISLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALKKYHQMRGE"
     gene            11976..12758
                     /gene="trpC"
                     /locus_tag="NBK22_001110"
     CDS             11976..12758
                     /gene="trpC"
                     /locus_tag="NBK22_001110"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCD2414306.1"
                     /translation="MTILAEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKTFINAIEQ
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYEKYGANAVSILTDEKYFGGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKPNHYYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS"
     gene            12758..13390
                     /locus_tag="NBK22_001111"
     CDS             12758..13390
                     /locus_tag="NBK22_001111"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCD2414307.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKGFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQTVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRDNE"
     gene            13383..14597
                     /gene="trpB"
                     /locus_tag="NBK22_001112"
     CDS             13383..14597
                     /gene="trpB"
                     /locus_tag="NBK22_001112"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCD2414308.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEEGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEGGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMAERGLAND"
     gene            14590..15318
                     /locus_tag="NBK22_001113"
     CDS             14590..15318
                     /locus_tag="NBK22_001113"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCD2414309.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIRYLQSIQQTLNN"
     gene            15640..16902
                     /gene="femA"
                     /locus_tag="NBK22_001114"
     CDS             15640..16902
                     /gene="femA"
                     /locus_tag="NBK22_001114"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCD2414310.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRYLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            16921..18180
                     /gene="femB"
                     /locus_tag="NBK22_001115"
     CDS             16921..18180
                     /gene="femB"
                     /locus_tag="NBK22_001115"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCD2414311.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
BASE COUNT         6454 a         2608 c         3333 g         5839 t
ORIGIN      
        1 gatatatttt gatttataac agaaataatt agaattgatg tgaaaaaatg aatcaggaag
       61 ttaaaaacaa aatattttca atcttaaaaa ttacgtttgc tacagcttta tttatttttg
      121 tagcaatcac attgtatcgg gagttatctg gtattaactt taaagatacg ttggttgaat
      181 ttagtaagat taaccgtatg tccttagtgt tactatttat tggtggtggg gcatcgcttg
      241 ttattctatc aatgtatgat gtgattttat ctagagcttt aaaaatggat atatccttag
      301 gcaaagtttt aagagtaagt tatatcatca atgcattgaa tgcgattgta ggtttcggtg
      361 gctttattgg tgcaggcgtt agagcaatgg tttataaaaa ctatacgcat gataaaaaga
      421 aattagttca ctttatatcc ttaatactta tttcaatgtt gacaggttta agcttattat
      481 cattgctaat tgtattccat gttttcgatg catctttaat cttagataag attacatggg
      541 taagatgggt attatatgta gtgtcatttt tcttaccatt attcattatt tattcaatgg
      601 ttagaccacc cgataaaaac aatcgttttg taggattgta ctgcacttta gtgtcgtgtg
      661 ttgaatggtt agcagctgca gttgtattat atttctgtgg tgtaattgtt gacgctcatg
      721 tatcattcat gtcctttatt gcaatattta tcattgctgc attatcaggt ttagtcagct
      781 ttattcctgg tggtttcggc gctttcgatt tagttgtatt actaggattt aaaactttag
      841 gtgtccctga ggaaaaagta ttattaatgc tacttctata tcgttttgcg tactattttg
      901 taccggtaat tattgcatta attttatcat catttgaatt tggtacatca gctaagaagt
      961 acattgaggg atctaaatac tttattcctg ctaaagatgt tacgtcattt ttaatgtctt
     1021 atcaaaagga tattattgct aaaattccat cattatcatt agcaatttta gtattcttta
     1081 caagtatgat cttttttgta aataacttaa cgattgttta cgatgcttta tatgatggaa
     1141 atcacttaac gtattatatt ctattggcaa ttcatactag tgcttgttta ttacttttac
     1201 tgaatgtagt tggtatttat aagcaaagta gacgtgccat tatctttgct atgatttcaa
     1261 ttttattaat cacagtggcg acattcttca cttacgcttc atatatttta ataacatggt
     1321 tagctattat ttttgttctg cttattgtag ctttccgtag agcacgtagg ttgaaacgcc
     1381 cagtaagaat gagaaatata gttgcaatgc ttttattcag tttatttatt ttatatgtta
     1441 accatatatt tattgctgga acgttatatg cattagatat ttatacgatt gaaatgcata
     1501 catctgtatt gcgctattac ttctggctta cgattttaat catcgctatc atcataggta
     1561 tgattgcatg gttgtttgat tatcaattta gcaaagtacg tatttcttct aaaattgaag
     1621 attgcgagga gattattaat cagtacggcg gtaattattt gagtcacttg atatatagtg
     1681 gtgacaagca gtttttcact aatgaaaata aaacagcatt tttaatgtat cgttataaag
     1741 caagttcatt agtggttctt ggagatccgt taggtgatga aaatgccttt gatgaattgt
     1801 tagaagcatt ctataattac gctgagtatt taggctatga tgttatattc tatcaagtta
     1861 cagatcaaca catgccttta tatcataatt tcggtaacca atttttcaaa ttaggtgaag
     1921 aagcaattat tgatttaacg caattttcaa cttcaggtaa aaaacgccgt ggatttagag
     1981 cgactttaaa taaattcgat gaacttaata tttcgttcga aattattgaa ccaccgtttt
     2041 caactgaatt tataaatgaa cttcaacatg taagtgattt atggctagat aatcgtcagg
     2101 aaatgcattt ctctgttggt caatttaatg aagaatactt atctaaagcg ccaattggtg
     2161 taatgcgaaa tgaagaaaat gaagtaattg cattttgtag tttaatgcca acatacttta
     2221 atgatgccat ttcagtcgat ttaattagat ggttgccaga gttagattta ccattaatgg
     2281 atggtctata cttgcatatg ttactttgga gtaaagaaca aggttataca aaatttaata
     2341 tgggtatggc aacgttatcg aacgttggtc aattgcatta ttcatattta agagaacgac
     2401 ttgcaggccg tgtctttgaa catttcaacg gtctatatcg tttccaagga ttacgtcgtt
     2461 ataaatctaa atataatccg aattgggaac cacgcttttt agtttatcgt aaagataatt
     2521 cgctttggga atcactttct aaagtaatgc gtgtaatacg tcacaaataa ttaaaatcca
     2581 agtgctaaga ggtatacagt tatccctgat gcgcttggat tttttggttt ctataaagaa
     2641 tgatttataa aaaacggtac agataagaat atttaagttt cctatctgta ccgtatttta
     2701 atattgaaat gagctaaagt taagtcgcct aacttttatt atcagtccaa tcagtttcat
     2761 caagtataaa tgttattgct tatttttgta ttcttggcgt attttttgtt cttctgcata
     2821 gcgctctgga tttttcttat aaaaatcttg gtgatagtct tcggctttgt aaaattgtga
     2881 cgctggtaat atttttgttg caattgcctt atcagcatta atcgtatttt taagctgctc
     2941 gatataagtc tcagcgagtt ctttttgatg atcattagtg tagaaaatag ctgtttgata
     3001 ttgaggacca cggtcttgat attgaccacc tgtatctaat gggtcaatga ctgagaaaaa
     3061 tatttctaat aacttattgt atgagaataa tgcaacatca tattgaattt caacagtttc
     3121 taaatgacca ctcgtacctg attttacttg ttcgtaagta ggattttcaa tatgtccgcc
     3181 catatatcca gaagttactt tttctatgcc gtcaaaggtg tcaaatggtt tcgtcataca
     3241 ccaaaagcaa cctccggcaa aataagctgt attaatattc atttttgaca tcctttcatt
     3301 agaccttagt acgatttatt aagaaatcac ttgctttttg aattgttttt atataacgtt
     3361 aatatgtgat tattatagta cacaaaaatc tatttttgaa gatataaggt aggtaaatat
     3421 ggataaagaa actaatgaca acgaatatag acgtcaaagt gaatatcgca cttcggcgcc
     3481 taagcgaaaa aagaagaaga aaattaggaa attacctatc attcttctga ttgttgtaat
     3541 tttacttatc gcattagttg tatatattgt acatagttac aatagcggtg tagaatatgc
     3601 caagaaacat gcgaaagatg ttaaagtaca tcaatttaat ggaccagtaa aaaatgatgg
     3661 taaaatttct attcttgtac tcggtgcaga taaagcacaa ggtggacaat caagaacaga
     3721 ttctatcatg gttgttcaat atgactttat caataaaaag atgaaaatga tgtctgtcat
     3781 gcgtgatatt tatgcagata ttccaggata tggaaaacac aaaattaatt cagcatacgc
     3841 tttaggtggt ccagagctac ttagaaaaac acttgataaa aatttaggaa ttaatcctga
     3901 atattatgca gtagttgatt ttactggatt tgagaaaatg attgatgaat taatgcctga
     3961 aggtgtacca attaatgtcg aaaaagatat gtcgaaaaat attggtgtat ctttgaaaaa
     4021 gggtaaccat aggttgaatg gtaaagaatt acttggttat gcaagattcc gtcacgaccc
     4081 tgaaggtgac ttcggacgcg tgcgacgtca gcaacaagtg atgcaaacat tgaaaaaaga
     4141 aatggttaat tttagaacag ttgttaaatt accaaaagtt gcaggtattt taagaggcta
     4201 tgtgaataca aacattcctg attcagggat tttccaaaca ggtttgagtt ttggtatccg
     4261 aggtgaaaaa gatgttaagt cattgactgt gccaatcaag aactcatacg aagatgtcaa
     4321 tacaaatact gatggtagtg cattacagat taataaaaac acaaataaac aagctattaa
     4381 agacttttta gatgaagatt aaaaataaac aaggcgattt ctatcatact cagatagaag
     4441 tcgccttgtt cgttttcaaa gaattaattg gcatgcatct tatgaagaat tattgatctg
     4501 actttcttac gccagccaca ccataatggt ttggtttcat ttcttctata acaacgtgaa
     4561 ttgcttgtct atttgccccc gttgtttttt ctacggcgtc agttacttcg ctaactaaat
     4621 tttttaattg ttcatccgaa cgaccttcta ataattttac attgacgatt ggcatcatcc
     4681 aaacctccta atcattatta atttgagtat atcatgtaat acttaaaaat tcatttgcaa
     4741 aataacagaa catttgttct aaaatagttg aaatagaaca cgtgttcgta tataataaga
     4801 ttcaagtaag ggaggaataa cagtgtataa ttatcattta ttagaagata gggatgttct
     4861 atgtattgac caaaaaagtt tttttgcgag tgtttcttgt attgaaaagg ggctagatcc
     4921 attagaaaca aagctagctg ttgttgcaga tactaagcgt cagggttctg taatattggc
     4981 tgcgacacct aaattaaaag aattaggcat caagacaggg tcgcgattgt ttgaaatacc
     5041 acatagaaat gatatttaca ttatcaatcc aagtatgcgt aaatatctta atgtttcagt
     5101 tgctatttct aagattgcat tgcgttatat tccacctgaa gatttacacc aatatagtat
     5161 tgacgaattt tttatggatg ttactgatag ctatcataga tttagttcta cagtacatgc
     5221 attttgcgaa agacttaaac gtgaaattta tgaagaaaca ggcatttatt gtactgtggg
     5281 cattggttct aatatgttat taagtaaaat tgctatggat gttgaagcga agcatagtca
     5341 aaatggtata gctgaatggc gatatcaaga tgtaccaacg aaattatggc caattcagcc
     5401 cttgcgagat ttttggggta ttaatcgtcg aacagaagcc aaattgaata aaagaggaat
     5461 ttttactata ggagatttag cgaaatatcc atataaattt ttaaaaaaag agttcggtat
     5521 tttaggtgtt gatatgcatc tacatgcgaa tgggatagat cagagtaaag tacgtgaaaa
     5581 gcacaagatc agcaatccat cgatatgcaa aagtcaaata ttaatgagag attatcattt
     5641 tgatgaagca aaagtagtaa tgcaagagtt aattgaagat gttgctagca gagttcgagc
     5701 aagaaaaaaa gtggcaagaa cgatacattt tgcctttggc tatagtgatg aaggcggtgt
     5761 acataagcaa tatactttga aagatccaac aaacttagaa aaagatattt ataaagtagt
     5821 aatgcatttc gcagataaat tatgtaataa acaagcacta tatcgtacgc taagtatatc
     5881 tttgagtcaa tttattaatg aggatgagcg acagttaagt ctgtttgaag atgaatacca
     5941 acgcaaacgt gacgaatgtc tagctaaaac gatagaccaa ttacatttga aatacggcaa
     6001 aggtattgtg tccaaagcag tatcgtttac agaagcaggt acaaaacacg gcagattagg
     6061 tttaatggct ggacataaaa tgtaatgact atacggttta agtaatatat aactgtgatt
     6121 cgtataaaat aagtctctaa agataaatat ttcatatatc acaatagatt ttcacaataa
     6181 tatctaagaa tacatggaat ttatcaaaag agacttaata attattggat ataacaatca
     6241 aaatcactca atgcttgcat accgcgttct cggtcagtag ggtttttgaa actaattttt
     6301 aaagcaccgt atatatcttc gcgtacttct aagattctta agttgcttat agatatgtta
     6361 tgtaaactca ggatataagt cactttactt atcatacctg attcatccgg aatgtctaca
     6421 tatagatcat acgcagtatt tagtccacct agttgtttag cgggtagtgc gtcgcgatac
     6481 gatttagctt gggcaaaaaa tgataacaat ttttcagaat cattgctttc aattagtctt
     6541 tctaaatctt gaaactgact ttttagctgt cgaatcattt ctaaaatata cgttttatta
     6601 ctcaaggtga tatctttcca catttgtgca ttactactag ctatacgagt gatatcacga
     6661 aaaccaccag ctgcaagttt attaactaaa tgatgttctt gaccgttctt ttgactaaca
     6721 tgaactaaac tagatgcaac gatatgaggt aaatgactta cgacgcttgt tacgtagtcg
     6781 tgttcttcag cagtagttac aataaattta gcaagagtag gtgataacag ttcttttaac
     6841 gtgtttgctg cttgctcatt tcttggctca ttgtagacta aaatataata agcgttttca
     6901 aataagtgct ttttagcatt tagtacacca gatttatgac taccagccat tggatgacca
     6961 ctgactaaat gaatattatg ctttaataaa ttgcattcgt gttgctgtat cattgcttta
     7021 gtactaccag tatcagaaac aataacacca ggtttagttg gcatatctat aagctcgcta
     7081 agatatttat ttgtgatagc aacaggtgtt gcataaataa ttacatcggc ttttttaata
     7141 gcttcactat aatttaaaca tttttcatta ataatgccga ttgatttagc tttatctaac
     7201 tgagaagtat ctgcatcgta tgcaataata ttagtattag ggttatggta ttttatattg
     7261 ctagcaagac ttccaccaat taatccaagc ccaacaaata aaactgttgt catataaatc
     7321 accttatttc gaaattttca gaataataac attgtaaatg agctgttgac acagtgcaat
     7381 agtaaataaa aatcgataat agcattaata gataaacgga gataaatcat ctacaataaa
     7441 gagtatagta acacaatggc aacggagggg taaatcaatg gaaccaatat tagaaatgat
     7501 taaaacatta acaggtatta atagtccttc aggagacaca gaagaagcaa ttcaatttgt
     7561 cgaaaaatat gcaaaagact tgggttatca aacaacacta acaaataaag gtgcgttatt
     7621 aataacagtg ccaggcaaaa atgatgaagt acaacgctgt attactgctc atgttgatac
     7681 tttaggtgca atggttaaag aaattaaaga agatggtcgc ttagcaatag aattaattgg
     7741 aggattcacg tataacgcga ttgagggtga atattgccaa attaaaactg atgctggtca
     7801 aatatataca ggaacaattt gtctgcatga aacaagtgtt catgtatata gaaataatca
     7861 tgaaatacct agagatcaaa agcatatgga aataagaatt gatgaagtaa ctacatcaga
     7921 agaagataca aagagtttag gtatttcagt aggtgatttt gttagctttg atccacgtac
     7981 agttatcacg tcatcaggtt ttattaaatc tcgtcattta gatgataaag ctagcgtagc
     8041 gatgatacta caattactaa agaaattaaa agaagagcaa ataatattac cacatacaac
     8101 gcaattttat atttctaata acgaagaaat aggttacggt gcaaatgcat caattgattc
     8161 gaaaatcaaa gaatatattg cattagatat gggcgcgttg ggagacggtc aagcatcgga
     8221 tgaatataca gtttctattt gtgccaaaga tgcttcaggt ccatatcata agcaattgaa
     8281 atcgcaccta gttaatcttt gcaaaataaa taacattcca tataaagtag acatatatcc
     8341 atattatggt tcagatgctt cagcagcttt acatgctggt gcggatatca gacatggttt
     8401 atttggcgct ggcattgaat catctcatgc aatggaacga acacatattg attctattaa
     8461 agcgacagag aaattactat atgcatattg cttatcacca attgagtaaa caattagtgt
     8521 tgacaaatgt gaacgaccta tgtaatataa tgaactataa aaataattag aattttctaa
     8581 agaaatagta gcagatatga aacgtagcaa atagaaagct aatgggtgat gggaattagc
     8641 acgccatatc ttgtgaattg gactttggaa aacaattgaa tgagttttga aagtgaacat
     8701 gaattatgtt aactaaggtg gcaccacggt aacgcgtcct tacaggtata tgcgttatgt
     8761 ggtgtctttt tatttagaca aaatgtagta gttaattaaa ggtagcaaca gaaagttagt
     8821 ggatgatgtg aactaacacc gagattaatg aaattgggtt ttgtctgcaa cagaaaaatt
     8881 atatatagta aagagtgaac tatgaatatt tcgaatattc ggttaattta ggtggtacca
     8941 cgcgtcagcg tcctttatat tgataaggat gctggcgctt ttttgaaagg agcgtataga
     9001 atggatatat tttataaaaa aataaaagca aatgtaacgc ccgaagtttt agcacaactt
     9061 cattccaaga agatcatttt ggaaagtaca aatcaacaac aaactaaagg tcgctattca
     9121 gttgttattt ttgatattta tggcacttta actttagata atgatgtatt atcagtaagt
     9181 actttaaaag aatcgtatca aatcactgaa agaccgtacc attatttaac gactaaaata
     9241 aatgaagact accataatat tcaagatgag caacttaagt cattaccatt tatatctgga
     9301 tatgttggga cgtgtagctt tgatttagta agacatgaat ttcctaaatt gcaatcaata
     9361 caattagaag atcacaagca gcacgatgta aggttatata tggttgaaca agtttatgta
     9421 tttgaccatt acaaagatga gttatatatc atcgcgacga atcaattttc aaattcaaca
     9481 aaatcagatc ttgagaatcg agttattaag tctatcgaag acttaactaa aatccaacca
     9541 ttcatgccta cacaagattt tgattttaaa actaaagaaa ttcaatcaaa catttctgaa
     9601 gaaagattta tcgaaatgat tcagtatttc aaagagaaaa taacagaagg ggatatgttc
     9661 caagttgtgc catcaagaat ttacaaatat gcgcatcatg ctagtcagca tttaaatcaa
     9721 ctttcgtttc aactgtatca aaatttaaaa cgacaaaacc caagtccata tatgtattat
     9781 cttaatatcg atcaaccata tattgtcggt agttctcccg aaagtttcgt aagtgtcaaa
     9841 gatcaaattg taacaactaa tcctattgca ggtacgattc aacgtggtga gacgacacaa
     9901 atagataatg agaatatgaa acaactactt aatgatccaa aagaatgcag cgaacatcgt
     9961 atgctagttg atttaggacg taatgatatt catagagtaa gtaaaatcgg tacctcaaaa
    10021 attactaaat taatggttat tgaaaaatat gaacatgtta tgcatatcgt aagtgaaatc
    10081 acaggtaaaa taaatcaaaa tttatcacca atgacagtta ttgcgaattt attaccaaca
    10141 ggtaccgttt caggtgcacc aaaattacgt gcaattgaaa gaatatatga acaatatcca
    10201 cataaacgag gcgtttatag tggtggtgtt ggatacataa attgtaatca taacttagat
    10261 tttgctttag caattcgaac gatgatgata gatgagcagt atatcaacgt agaagctggt
    10321 tgtggcgttg tatatgattc tattcctgaa aaagaactga atgaaacgaa attgaaagct
    10381 aaaagcttat tggaggtgag gccatgatct tagttgtaga taattatgat tcctttacat
    10441 ataacctagt ggatattgtt gctcaacata ctgacgtcat tgttcaatat cctgatgatg
    10501 ataatgtgct gaatcaatcg gtggacgctg ttattatatc tcctggtcca gggcatccat
    10561 tagacgatca acagttaatg aaaatcatat caacctatca acacaaaccc attttaggta
    10621 tttgcctagg tgctcaggca ctgacttgtt attacggtgg agaagtcatt aaaggcgaca
    10681 aggttatgca cggaaaagtt gatacactaa aggttatatc gcatcatcaa catctgttat
    10741 atcaagatat accagaacag ttttcaatta tgagatatca ttcattaata agtaaccctg
    10801 acaattttcc agaagaattg aaaattactg gacgtaccga agattgtata cagtcatttg
    10861 agcataaaga aagaccgcat tacggtattc agtaccatcc tgaatcattt gctacagact
    10921 atggtgtcaa aataattaca aattttatta atctagtgaa ggaaggatga aaaccatgac
    10981 attactaaca agaataaaaa ctgaaactat attacttgaa agcgacatta aagagctaat
    11041 cgatatactt atttctccta gtattggaac tgatattaaa tatgaattac ttagttccta
    11101 ttcggagcga gaaatccaac aacaagaatt aacatatatt gtacgtagct taattaatac
    11161 aatgtatcca catcaaccat gttatgaagg ggctatgtgt gtgtgcggca caggtggtga
    11221 caagtcaaat agtttcaaca tttcaacgac tgttgctttt gttgtagcaa gtgctggcgt
    11281 aaaagttata aaacatggta ataaaagtat tacctcaaat tcaggtagta cggatttgtt
    11341 aaatcaaatg aacatacaaa caacaactgt tgatgataca cctaaccaat taaatgaaaa
    11401 agaccttgta ttcattggtg caactgaatc atatccaatc atgaagtata tgcaaccagt
    11461 tagaaaaatg attggaaagc ctacaatatt aaaccttgtg ggtccattaa ttaatccata
    11521 tcacttaacg tatcaaatgg taggcgtctt tgatcctaca aagttaaagt tagttgctaa
    11581 aacgattaaa gatttaggta gaaaacgtgc aatcgtttta catggtgcaa atggtatgga
    11641 tgaagcaaca ctatctggtg ataatttgat atatgaattg actgaagatg gagaaatcaa
    11701 aaattacaca ttaaatgcga ctgattatgg tttgaaacat gcgccgaata gtgattttaa
    11761 aggcggttca cctgaagaaa atttagcaat ctcccttaat atcttgaatg gtaaagatca
    11821 gtcaagtcga cgtgatgttg tcttactaaa tgcgggttta agcctttatg ttgcagagaa
    11881 agtggatacc atcgcagaag gcatagaact tgcaactaca ttgattgata atggtgaagc
    11941 attgaaaaaa taccatcaaa tgagaggtga ataatatgac gattttagca gaaattgtta
    12001 aatataaaca gtcactttta caaaatggct attatcaaga caaacttaat accttgaaaa
    12061 gtgtgaagat tcagaataaa aaaactttta taaacgcaat tgaacaggaa ccaaagctag
    12121 caattattgc agaaatcaaa tcgaagagtc ctacagttaa tgacttacct gaacgagatt
    12181 tatcgcaaca aatctcagat tatgaaaagt acggtgcaaa tgccgtgtcc attttaactg
    12241 atgaaaagta ctttggtggt agttttgaaa gattacaagc attgacgaca aaaacaacat
    12301 tacccgtatt atgcaaagac tttattatag acccgcttca aattgatgtt gctaaacaag
    12361 ctggtgcatc tatgatttta ttgatcgtta acatcttatc tgataaacaa ttgaaagatt
    12421 tatataacta cgctatatcg caaaatctag aagtgttagt tgaagtacat gatcgccatg
    12481 aattagaacg tgcctataag gttaatgcta aattgattgg tgtaaataac agggacttaa
    12541 aacgatttgt tacaaatgtg gaacatacaa atactatttt agaaaataaa aaaccaaatc
    12601 attattatat ttctgaaagt ggtattcacg atgcatctga tgtaagaaaa atcttgcata
    12661 gtggtatcga tggcttacta ataggtgagg cgcttatgcg ttgtgacaat ctatctgaat
    12721 ttttaccaca actgaaaatg caaaaggtga agtcatgatg aaattgaaat tttgtggctt
    12781 tacatcaata aaggatgtta cagcggccag tcaattacct attgatgcga taggtttcat
    12841 ccattatgaa aaaagtaaaa ggcatcaaac aattacccaa ataaaaaagt tagcgtctgc
    12901 tgttccaaat catatcgata aagtatgtgt catggtaaat cctgatttaa caacaattga
    12961 acacgtatta agcaatacgt caattaacac aatacagtta cacggcacag aatctattga
    13021 ttttatacag gaaattaaaa agaaatattc aagcattaaa atcactaaag ctttagctgc
    13081 agatgaaaac ataatccaaa acataaataa atataaaggg ttcgtagatt tatttattat
    13141 cgacacaccc tcagtgtcgt atggtggtac cggtcaaaca tatgactgga ctattttgaa
    13201 gcacataaaa gacatacctt atttgatagc aggaggcatt aactctgaaa atattcaaac
    13261 agttaatcaa cttaaattat cacatcaagg ttatgatctt gcatcaggta tagaagtaaa
    13321 tgggcgaaaa gatatagaaa aaatgacagc aattgtaaat attgtgaaag gagatagaga
    13381 caatgaataa acaaatacaa acagaagcag atgaattagg tttctttggt gaatatggag
    13441 ggcaatatgt tccagaaaca ttaatgccag caattattga gttgaaaaaa gcttataaag
    13501 aggcaaaagc agacccagag tttcaaagag aactggaata ctatttatca gagtatgtag
    13561 gacgcgcgac accacttaca tatgctgcat catatactga aagcctaggt ggcgctaaaa
    13621 tatatttgaa acgagaggat ctaaatcata caggcgccca taaaattaat aatgcgttag
    13681 gtcaagcgtt gcttgctaaa agaatgggca agaagaagct tgttgctgaa actggtgcgg
    13741 gtcaacatgg tgtagctagt gctacggttg ctgcattatt tgatatggaa cttgttgtct
    13801 ttatgggaag tgaagatatt aaaagacaac aacttaatgt atttagaatg gaattacttg
    13861 gtgcaaaggt tgtggcagtt gaagaaggtc aagggacttt atcggatgca gttaataaag
    13921 cattgcaata ttgggtaagt catgtagatg atacacatta tttattaggt tctgcattag
    13981 gtccagaccc attcccaacg attgttagag attttcagag tgtcattggt aaagaaataa
    14041 aatcacagat attgaagaaa gaaggtcgac ttccggatgc aattgtagca tgtatcggtg
    14101 gtggctcaaa tgcaatcggt acattttatc catttattaa agatgatgtt gcattatacg
    14161 gtgttgaagc cgcaggtcaa ggcgaggata ctgataaaca tgcacttgca attggcaaag
    14221 gatcacctgg cgtattacat ggtactaaaa tgtatttaat tcaagatgaa ggtgggcaag
    14281 tgcaactagc acattctatt tcagcaggac ttgattatcc tggtattggg cctgaacatt
    14341 cttattacca cgacattggt agagtaactt ttgaaaatgc aagtgataca caagcaatga
    14401 atgctttaat caactttaca aaacatgaag gtattatacc tgcaattgaa agtgcacatg
    14461 cactgagtta tgttgaaaga ctagcgccta cgatgtcgaa agaagatatt attgtagtaa
    14521 ctatttctgg acgtggcgat aaagatatgg aaacaattag acaatatatg gcagagcgag
    14581 gtcttgcaaa tgactaaatt atttatacct tatattatgg gcaataaaga tttgattgaa
    14641 aatgcaacat tgttgagtga aaatggtgca gatataattg aaattggtgt acctttctct
    14701 gatccggttg ctgatggtcc agttatcatg gaagcaggtc aacaagcgat taaacaaggc
    14761 atcacgatag attatatttt caatcaatta gaaaaacatg gtgatcaaat taagtgtaac
    14821 tatgtattaa tgacgtatta taatattatt tgtcattatg gagaacaagc gttttttgaa
    14881 aaatgtcgag atactggtgt ctacggctta attattcctg atttaccata tgaattatcg
    14941 cagcgtttaa aacaacaatt tagtcactat ggcgtcaaaa tcatatcgtt agttgcgatg
    15001 actactgatg acaaacgtat aaaagatatc gtatctcatg cggaaggctt tatttatact
    15061 gtgacgatga atgcgacaac aggacaaaac ggtgcgtttc atccagaatt aaaacgaaaa
    15121 attgagtcaa ttaaagcgat agccaatgtg ccagttgtcg caggatttgg tataagaaca
    15181 ccacaacatg ttgcagatat aaaagaggtt gcagatggca tcgtcattgg tagcgaaatc
    15241 gttaagcgat ttaaatctaa cacgcgtgag gaaatcatta gatatttaca atctatccaa
    15301 caaacattga ataattaagt ttacttgatt taaaaaaatt aggcgaatac tgtttgaaaa
    15361 agtgaaaaac ggtgaattat aaaattgaat agaaatgata caaaagtaat atgagcaaac
    15421 ccaaacgttc atattacttt ttttgaaatt gtatgcaaaa atctaaatat tactataaaa
    15481 gtatacgcaa ttaaagcgtt tatgttttag ttttaacatt aactattgta tacttattta
    15541 gattagattt attatttttg acatttgcag aggggaaata gaaaaactgc aaatacggaa
    15601 atgaaattaa ttaacgagag acaaatagga gtaatgataa tgaagtttac aaatttaaca
    15661 gctaaagagt ttggtgcctt tacagatagc atgccataca gtcatttcac gcaaactgtt
    15721 ggccactatg agttaaaact tgctgaaggt tatgaaacac atttagtggg aataaaaaac
    15781 aataataacg aggttattgc agcttgctta cttactgctg tacctgttat gaaagtgttc
    15841 aagtattttt attcaaatcg cggtccagtg atcgattatg aaaatcaaga actcgtacac
    15901 tttttcttta atgaattatc aaaatatgtt aaaaaacatc gttgtctata cctacatatc
    15961 gatccatatt taccatatca atacttgaat catgatggcg agattacagg taatgctggt
    16021 aatgattggt tctttgataa aatgagtaac ttaggatttg aacatactgg attccataaa
    16081 gggtttgatc ctgtgctaca aattcgttat cactcagtgt tagatttaaa agataaaaca
    16141 gcagatgaca tcattaaaaa tatggatgga cttagaaaaa gaaacacgaa aaaagttaaa
    16201 aagaatggtg ttaaagtaag atatttatct gaagaagaac tgccaatttt tagatcattt
    16261 atggaagata cgtcagaatc aaaagctttt gctgatcgtg atgacaaatt ttactacaat
    16321 cgcttaaaat actacaaaga tcgtgtgtta gtacctttag cgtatatcaa ttttgatgaa
    16381 tatattaaag aactaaatga agagcgtgat attttaaata aagatttaaa taaagcatta
    16441 aaggatattg aaaaacgtcc tgaaaacaaa aaagcgcata acaagcgaga taacttacaa
    16501 caacaacttg atgcaaatga gcaaaagatt gaagaaggta aacgtctaca agaagaacat
    16561 ggtaatgaat tacctatctc tgctggtttc ttctttatca atccatttga agttgtttat
    16621 tatgctggtg gtacatcaaa tgcattccgt cattttgccg gaagttatgc agtgcaatgg
    16681 gaaatgatta attatgcatt aaatcatggc attgaccgtt ataatttcta tggtgttagt
    16741 ggtaaattta cagaagatgc tgaagatgct ggtgtagtta aattcaaaaa aggttacaat
    16801 gctgaaatta ttgaatatgt tggtgacttt attaaaccaa ttaataaacc tgtttacgca
    16861 gcatataccg cacttaaaaa agttaaagac agaatttttt aggaagggaa ttatcaaaac
    16921 atgaaattta cagagttaac tgttaccgaa tttgacaact ttgtacaaaa tccatcattg
    16981 gaaagtcatt atttccaagt aaaagaaaat atagttaccc gtgagaatga tggctttgaa
    17041 gtagttttat taggtattaa agacgacaat aacaaagtaa ttgcagcaag ccttttctct
    17101 aaaattccta ctatgggaag ttatgtttac tattcgaatc gtggtccagt aatggatttt
    17161 tcagatttag gattagttga ttattattta aaagagttag ataaatattt acagcaacat
    17221 caatgtttat atgttaaatt agatccgtat tggttatatc atctatatga taaagatatc
    17281 gtgccatttg aaggtcgcga gaaaaatgat gccttagtaa acttgtttaa atcacatggt
    17341 tacgagcatc atggctttac aactgagtat gatacatcga gccaagtacg atggatgggc
    17401 gtattaaacc ttgaaggtaa aacacccgaa acattgaaaa agacatttga tagtcaacgt
    17461 aaacgtaata ttaataaagc gataaactat ggtgttaaag tcagattcct tgaacgtgat
    17521 gagttcaatc ttttcttaga tttatatcgt gaaactgaag agcgtgctgg atttgtatca
    17581 aaaacagatg attattttta taactttatt gacacatatg gagataaagt attagtacca
    17641 ttagcatata ttgaccttga tgaatatgtg ttaaagttgc aacaggaatt gaatgacaaa
    17701 gaaaatcgtc gtgatcaaat gatggcgaaa gaaaacaaat cagataagca aatgaagaaa
    17761 attgcagaat tagataagca aattgatcat gatcagcatg aattattgaa tgcaagtgaa
    17821 ttgagcaaaa cggacggccc aattctaaac cttgcttctg gcgtttattt tgcaaatgca
    17881 tatgaagtga attatttctc tggtggttca tcagaaaaat ataatcaatt tatgggacca
    17941 tacatgatgc attggtttat gattaactat tgcttcgata atggctatga tcgttataat
    18001 ttctatggtt tatcaggtga ttttacggaa aacagtgaag attatggcgt ataccgcttt
    18061 aaacgtggat ttaatgtaca aatcgaagaa ttaatagggg atttctataa accaattcat
    18121 aaagtgaaat attggttgtt cacaacattg gataaattac gtaaaaaatt aaagaaatag
    18181 atgtaaatga tgttagcgct tatagttatt gatactatag gctctttttt atat
//
LOCUS       DAHHYJ010000044        18110 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000044 DAHHYJ010000000
VERSION     DAHHYJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.044"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            136..2409
                     /gene="recJ"
                     /locus_tag="NBK22_001116"
     CDS             136..2409
                     /gene="recJ"
                     /locus_tag="NBK22_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCD2414312.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     AIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCLTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     QVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILD
                     YRSKQKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            2431..2949
                     /locus_tag="NBK22_001117"
     CDS             2431..2949
                     /locus_tag="NBK22_001117"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCD2414313.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEKIK
                     DYDVMSLISYDE"
     gene            3377..5566
                     /locus_tag="NBK22_001118"
     CDS             3377..5566
                     /locus_tag="NBK22_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCD2414314.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEVEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
     gene            5578..6030
                     /locus_tag="NBK22_001119"
     CDS             5578..6030
                     /locus_tag="NBK22_001119"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCD2414315.1"
                     /translation="MKVVVQRVKEASVTNDTLNNQIKKGYCLLVGIGQNSTEQDADVI
                     AKKIANARLFEDDNNKLNFNIQQMNGEILSVSQFTLYADVKKGNRPGFSNSKNPDQAV
                     KIYEYFNDALRAYGLTVKTGEFGTHMNVSINNDGPVTIIYESQDGKIQ"
     gene            6027..6902
                     /locus_tag="NBK22_001120"
     CDS             6027..6902
                     /locus_tag="NBK22_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD2414316.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            7363..8625
                     /locus_tag="NBK22_001121"
     CDS             7363..8625
                     /locus_tag="NBK22_001121"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCD2414317.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            8641..10407
                     /gene="aspS"
                     /locus_tag="NBK22_001122"
     CDS             8641..10407
                     /gene="aspS"
                     /locus_tag="NBK22_001122"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCD2414318.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            10485..10675
                     /gene="ssrS"
                     /locus_tag="NBK22_001123"
     ncRNA           10485..10675
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NBK22_001123"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10740..10868
                     /locus_tag="NBK22_001124"
     CDS             10740..10868
                     /locus_tag="NBK22_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414319.1"
                     /translation="MGLIAFNKCFFVEFLKNNQSNNAKYKILSYYVLMYKSVKEVK"
     gene            10868..11641
                     /locus_tag="NBK22_001125"
     CDS             10868..11641
                     /locus_tag="NBK22_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCD2414320.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMSKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(11802..13076)
                     /locus_tag="NBK22_001126"
     CDS             complement(11802..13076)
                     /locus_tag="NBK22_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCD2414321.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            13161..13583
                     /locus_tag="NBK22_001127"
     CDS             13161..13583
                     /locus_tag="NBK22_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD2414322.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            13683..13865
                     /locus_tag="NBK22_001128"
     CDS             13683..13865
                     /locus_tag="NBK22_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD2414323.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            13905..14051
                     /locus_tag="NBK22_001129"
     CDS             13905..14051
                     /locus_tag="NBK22_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414324.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(14288..15301)
                     /locus_tag="NBK22_001130"
     CDS             complement(14288..15301)
                     /locus_tag="NBK22_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD2414325.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGIYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDDEAQYLASSMAQVMVSITRGRMQPVQPPTHELQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            15613..16755
                     /locus_tag="NBK22_001131"
     CDS             15613..16755
                     /locus_tag="NBK22_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD2414326.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            16756..17874
                     /gene="mnmA"
                     /locus_tag="NBK22_001132"
     CDS             16756..17874
                     /gene="mnmA"
                     /locus_tag="NBK22_001132"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCD2414327.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPSQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
BASE COUNT         6515 a         2534 c         3487 g         5574 t
ORIGIN      
        1 caaaatatat ataaataagt tcccgcattc atttttaaaa aatggtataa ttaaagtctt
       61 gattatttag ataagtgctt tttatttaaa gtgattttag atataatgtc ttttggaaat
      121 gaggcgtaaa tatatatgat taaaccgaag tataagtgga aattaactaa accagctgaa
      181 tacataagtg atgaattaac atcaaaatta aagttaacgc caatcgtaaa aaaaatatta
      241 gaaagtaaat cgattattga tgaacaagca atagaatcca tcatttcaga tacagatatc
      301 aatcatgatg cattgcaatt gagcgatatg actaagacta ttgaaagaat taaaagagcg
      361 atagcgaatg atgagaaaat cctagtttat ggtgattacg atgctgacgg tgttacctca
      421 actacaattt tagtgacaac attacaactt ttaggtgcac aagtaggatg gcatattcct
      481 aaccgcttta ccgaaggata tggaccaaac gaactagcat ttagaaatgc tcatgatgaa
      541 ggcataacat taatcataac tgttgataat ggcatacaag gtcataatga aattaaaatg
      601 gttcaagatt taggagttga tgtcattgta actgatcatc atgaaattgg tagtacatta
      661 ccagaagcat atgcaatcgt acatccaatg catccatcgt ttaattatcc gttccaacaa
      721 ttgtgtggtg caggtgttgc atacaaatta gcacaagcat tgattgaaaa tgtacctgat
      781 tattttaaag cattagttgc gataggtacc attgcggatt tagtatcctt aactgatgaa
      841 aatcgatcat tagtgaaaca aggattaaaa gtcttaaatg atcaatgtct gacctctgtt
      901 aaagcattgc taaaagaagc gggatacaat gacaatattg atgaagaaac aattggtttt
      961 attattggac ctagacttaa tgctgtaggt cgtttagatg acgcatcatt agcttgcgaa
     1021 ttgttgatga ctgacgacga agaagaagct gcatttttag cagaacaagt tgaacatttt
     1081 aaccgtgaaa gaaaagatat tgtagcaact atcacagaag aagcaatggc tatggcagaa
     1141 acgaaagtta aaaagggaga tttattttta cttttagcaa aagaaaattg gcatgaaggt
     1201 gtcttaggta ttgttgcttc gaaaattgtc gaaacatttg ctttgccaac attaatttta
     1261 aatattgata gggagcaaaa tcatgcaaaa ggttccgcca gatcaattga tcaagtgtcg
     1321 atgtttgaaa tactatcagc acatcaagag ttaatcgcta aatttggcgg ccatcatatg
     1381 gctgcaggta tgacgatgga tattgaaaat atagaatcgt tagctgaagg tttaaataaa
     1441 tggatgaaag aattatctaa aacaacttcg ttagatcccg taaagcaagt agatgtatta
     1501 ttaacagaaa atgatataac gattaaaaat attcgtgata tgaatcgttt aagacccttc
     1561 ggaacagatt tttctagacc tatttttgaa atggatgatt tatcagtttc ttcagttaag
     1621 gcgataggtc aacaaaaaaa tcatctaaaa ttgacacttg gtgaaagtaa tatcgccgcg
     1681 ctattttggc aaaatggaca cttagaacct gagttacaag atgaacagcc aattaatata
     1741 ttaggatctg ttcaaatcaa cgaatggaat ggtaatcaat caccgcaaat aattattcaa
     1801 gatattgcga tgaatgaaca gcaaatatta gattatagaa gtaagcaaaa aagtttacct
     1861 tttacagaaa atgatgaaaa tattgtcgtg cttattcatc ctaaaagtga taaagtaaat
     1921 gcgaatgaat attattatgg tgaagaaatt aaacaacaaa ctgataaagt agtattaaga
     1981 gatttaccaa cgtcaatgga agacttgtct aattccttgc aacaactgca attttctcaa
     2041 ctttatatag ttttgcaaca taatcattcg atttacttcg atggtatacc taatatggat
     2101 atttttaaaa agtgttataa agcattaata actaaacaag aaacaaatat ccagaaagag
     2161 ggtatgttat tgtgtcaaca tttaagtgtg aaaccagata cacttaaatt catgttgaaa
     2221 gttttcttag acttaaaatt tgtaacacaa gaagatggtt taattcgaat caatcaacaa
     2281 cctgataaaa gatcgattga ttccagcaaa gtatatcaat taagacaaca acgtatggat
     2341 gttgaaaagc aattattata tcaagatttt tcagaaataa aaaattggat aaagtcacaa
     2401 ttgtcgtgag caatttagga ggaaatatta atggatttaa agcaatacgt atcagaagtt
     2461 caagattggc cgaaaccagg tgttagtttc aaggatatta ctacaattat ggataatggt
     2521 gaagcatatg gctatgcaac agataaaatt gtagaatacg caaaagacag agatgttgat
     2581 atcgttgtag gacctgaagc gcgtggcttt atcattggct gtcctgtagc ttattcaatg
     2641 gggattggct ttgcacctgt tagaaaagaa gggaaattac ctcgtgaagt cattcgttat
     2701 gagtatgacc tagaatatgg tacaaatgtt ttaacaatgc acaaagatgc aattaaacca
     2761 ggtcaacgtg tgttaattac agatgattta ttagctactg gtggtacgat tgaagcagca
     2821 ataaaattag ttgaaaaatt aggcggtatc gtagtaggta ttgcatttat aattgaattg
     2881 aaatatttaa atggtattga aaaaattaaa gattacgatg ttatgagttt aatctcatac
     2941 gacgaataat aaataatata attttatcaa atgaaatcct tcatcaaatg tataagaacc
     3001 aatgacttaa ttaaaaaagt tgtttaagtt ttcttaacat gagatgttag gattttttat
     3061 ttactgaaaa tgttagatga ttgagcatta taccttaata acatcgttta tttatttcat
     3121 aaattgtagt atcatagaac taatatttaa aaaatgaaac agtagattta ggtcgaattt
     3181 ttgtaaaagt tttaaaagta ggaatagtat acgaattaaa ctcgctcaag taaaattaat
     3241 attacgatta atgacgacag gataaatatt tatcgtcgac ggacgtatga ttggtgtggg
     3301 acaaatacta ttcaacaaga gtacctaaat cattgtttaa ggcgaagtaa taaatatgaa
     3361 tggggtgtat catataatga acaacgaata tccatatagt gcagatgaag ttcttcacaa
     3421 agcaaaatca tatttgtcag cagatgaata tgagtatgtt ttaaaaagct atcatattgc
     3481 ttatgaagca cataaaggtc agttccgaaa aaacggatta ccatacatta tgcatcctat
     3541 acaagttgca ggtattttaa cagaaatgcg attagacgga ccgacgattg tcgcaggttt
     3601 tttgcatgat gtaattgaag atacaccgta tacatttgaa gatgtaaaag aaatgttcaa
     3661 tgaagaagtt gctcgaattg ttgatggtgt gacgaagctt aaaaaagtaa aataccgctc
     3721 aaaagaagaa caacaagctg aaaatcatcg caagttattt attgcgattg ccaaagatgt
     3781 acgcgtaatt ttggtgaaat tagcagacag attacataat atgcgtacct tgaaagccat
     3841 gccgcgtgaa aaacaaatta gaatttctcg agaaacatta gaaatttatg cacctttagc
     3901 acatcgtctt ggtattaata caattaaatg ggaactagaa gatacggctc ttcgttatat
     3961 tgataatgtg caatatttta gaatagtcaa tttaatgaag aagaaacgta gtgaacgtga
     4021 agcgtatatc gaaacggcta ttgatagaat acgtactgaa atggaccgaa tgaatatcga
     4081 aggcgatata aatggtagac ctaaacatat ttacagtatt tatcggaaaa tgatgaagca
     4141 gaaaaaacaa tttgatcaaa tttttgattt gttggcgata cgtgttattg tcaattctat
     4201 taatgattgt tatgcgatac ttgggttggt gcatacgtta tggaaaccga tgccaggacg
     4261 ttttaaagat tatattgcaa tgcctaaaca aaatttgtat cagtcattgc atactacagt
     4321 agtaggtcca aatggagacc cgctcgaaat ccaaatacga acgtttgata tgcacgaaat
     4381 tgctgagcat ggtgttgcag cacactgggc ttacaaagaa ggtaaaaaag taagtgaaaa
     4441 agatcaaact tatcaaaata agttaaattg gttaaaagaa ttagctgaag cggatcatac
     4501 atcgtctgac gctcaagaat ttatggaaac cttaaaatat gacttacaga gtgacaaagt
     4561 atacgcattt accccagcga gtgatgttat tgagttgcca tatggtgctg tgccgattga
     4621 ttttgcttat gcgattcaca gtgaagtagg taataagatg attggtgcca aggtgaatgg
     4681 caaaattgta ccaattgact atattttaca aacaggcgat attgttgaaa tacgtactag
     4741 taaacattca tatggaccaa gtcgtgattg gttgaaaatt gttaaatcgt ctagtgccaa
     4801 aggtaaaatt aaaagtttct tcaaaaaaca agatcgttca tctaatattg aaaaaggccg
     4861 aatgatggtt gaagttgaaa taaaagagca aggatttaga gtcgaagata ttttgacaga
     4921 gaaaaatatt caggttgtta atgaaaaata taactttgca aatgaagatg atttattcgc
     4981 agctgtagga tttggcggcg tgacatcctt acagattgtt aataaattaa ctgaaagaca
     5041 acgtatttta gataaacaac gtgctttaaa tgaagcacaa gaagttacga aatcattgcc
     5101 tattaaagac aacatcatta ctgatagtgg tgtctatgta gaaggtttag aaaatgtact
     5161 tatcaagttg tcaaaatgtt gtaatcctat accaggtgat gatattgtag gttatatcac
     5221 caaaggtcac ggtattaaag tacatcgcac tgattgccca aatattaaga acgaaactga
     5281 acgactaatt aatgttgaat gggtaaaatc aaaagacgca actcaaaaat atcaggttga
     5341 tttagaggta actgcgtatg accgaaatgg cttgttgaat gaagtactac aagctgttag
     5401 ctcgacagcc ggcaatttaa ttaaagtttc aggacgttca gatattgata aaaatgcaat
     5461 aataaatatt agtgtcatgg tgaaaaacgt gaatgatgtt tatcgtgtgg tagaaaagat
     5521 caaacaactt ggtgatgttt atacagtaac aagagtttgg aactagaggt gcaaaatatg
     5581 aaagtagttg tacaaagagt taaagaagca tcggtgacga atgatacatt aaataatcaa
     5641 atcaaaaaag gatattgttt attagtcggt atcggtcaga actctacaga gcaagatgca
     5701 gatgtaattg caaagaaaat tgctaatgca agattatttg aagatgacaa taataaatta
     5761 aactttaata tccaacaaat gaatggtgaa atactatcag tttcacaatt tactctctat
     5821 gcagatgtaa aaaaaggtaa ccgtccaggt ttctcaaatt ctaaaaatcc tgatcaagcg
     5881 gtaaaaattt atgagtattt taatgatgcg ctacgagcgt atggtcttac tgtgaaaaca
     5941 ggtgaatttg gaacacacat gaatgttagc ataaataatg atggtccagt cactattatt
     6001 tatgaaagtc aggacggcaa aattcaatga aaaaaataga ggcatggtta tctaaaaagg
     6061 gtcttaaaaa taaacgtact ctaatagtag tgattgcctt tgtcttattt atcatctttt
     6121 tatttttatt gctgaatagc aatagtgaag atagtgggaa catcacgata actgaaaatg
     6181 ctgaattacg tacaggtcca aacgctgcgt atccagtcat atataaagtt gagaaaggtg
     6241 accattttaa aaagattggt aaagtaggta aatggattga agttgaagat acatccagta
     6301 atgaaaaagg ttggatagct ggatggcaca caaatttaga tattgtcgcg gataatacga
     6361 aggagaaaaa tcctttgcaa ggtaaaacaa tagtgcttga tcctggtcat ggaggtagtg
     6421 accagggtgc ttcaagcaat actaaatata aaagtttaga aaaagactat acgttgaaaa
     6481 cagcaaaaga attgcagcgt actttagaaa aagaaggcgc aactgttaag atgacaagaa
     6541 cagacgatac atatgtttca ctagaaaatc gtgatatcaa aggcgatgcc tatttgagta
     6601 tacataatga tgcattagaa tcatctaatg caaatggaat gacagtttat tggtatcatg
     6661 ataatcaaag agctttagca gatacgttag acgctacgat tcagaagaaa ggtctacttt
     6721 ctaatcgcgg ttcaagacaa gaaaattatc aagtgttaag acaaacaaaa gttcctgctg
     6781 ttttattaga attaggttat attagtaacc caactgatga aacgatgatt aaagatcaat
     6841 tacatagaca aattttagaa caagcaattg ttgatggcct taaaatttat ttttctgcgt
     6901 agggcttgca aaaatatgtg aaagtagtta tcattgatat tgaattttat aactaaaacc
     6961 gttagtattc ttgaaatggt aaatgaaata ggtagcaatc taactaagat tgtgtaggaa
     7021 tataatccat agactgaaag attatgctga gtagtttata tacattgaac acaagaagag
     7081 gtgctttatg aaaagtaaag ccgttaaacg tacgttaaac gttttgagtg ggtttattta
     7141 atgcacgctt ataaaaagta atgatgatta caattaggca tgttttttaa accaatgagg
     7201 gtggcaacac ggatattcgt cccttgtgca gtttcaggct gtgcaagggc tttttctttt
     7261 gcttttaaaa ttatgattta tcatgcaaag ttaagtgatc gtatatagtt ttagttttaa
     7321 aaaggtaatt aaataaaata gtttgccgag ggagatgtca aaatgattaa aatacctaga
     7381 gggacgcagg atattttacc tgaagattca aagaaatggc gttacattga aaatcaatta
     7441 gatgaattaa tgacatttta taattataaa gaaataagaa caccaatttt tgaaagtaca
     7501 gatctttttg caagaggtgt tggtgattca accgatgtcg tacaaaaaga aatgtataca
     7561 tttaaagata aaggcgatag aagtattaca ttaagacctg agggaacagc tgcagttgtg
     7621 cgttcatata ttgaacataa aatgcaaggt aatccaaacc aaccaattaa actttattac
     7681 aatggaccga tgtttagata tgaacgtaag caaaaagggc gctatcgtca atttaatcaa
     7741 tttggtgtag aagctattgg tgctgaaaat cctagcgtag atgcagaagt attagctatg
     7801 gttatgcata tttatcaatc atttggatta aaacatttaa agcttgttat taatagtgta
     7861 ggggatatgg cgtctcgaaa agaatataac gaagcgttag tgaaacactt tgaaccagta
     7921 attcatgaat tttgttcaga ttgtcaatca cgtttgcata caaatccgat gcgaattttg
     7981 gattgtaaag ttgaccgtga taaagaagcg attaagactg cacctagaat cactgatttc
     8041 ttaaatgagg aatctaaggc atattatgaa caagtaaaag cttatttaga tgatttaggt
     8101 attccatata ttgaagatcc taacttagtt cgtggattgg attattatac acatacagca
     8161 tttgaattaa tgatggataa ccctaactat gatggtgcca ttacaacgct ttgtggtggt
     8221 ggccgttata atggtttatt agaattgcta gatggtccaa gtgaaacagg tattggtttt
     8281 gcgctaagta tagaacgatt attgcttgca ctagaagaag aaggtatcga attagatatt
     8341 gaagaaaact tagatttatt cattgttaca atgggtgatc aagcagatcg atatgctgtg
     8401 aagttattaa atcatttgag acataatggt attaaagcag ataaagacta cttacagcgt
     8461 aaaattaaag gacaaatgaa acaagcagac cgtttaggtg ccaagtttac aatcgttatt
     8521 ggtgatcaag aattagaaaa taataaaatc gatgttaaaa atatgacaac tggtgaatct
     8581 gaaacaattg aattagacgc attagtcgaa tattttaaga agtagagagg gcgttaaaat
     8641 atgagtaaga gaacaactta ttgtggatta gttactgaag catttttagg acaagaaatt
     8701 acattaaaag gatgggttaa caatcgtcgt gacctaggtg gattgatttt cgttgattta
     8761 agagatagag aaggaattgt acaagtcgtg tttaatcctg cattttcaga agaggcattg
     8821 aaaattgctg aaacagtacg ttctgaatat gttgtagaag ttcaaggtac agttacgaag
     8881 cgtgaccctg aaacagttaa tcctaaaatt aaaactggcc aagttgaagt acaagttaca
     8941 aatattaaag tgattaataa atctgagaca ccaccatttt ctataaatga agaaaatgtt
     9001 aacgttgatg aaaatattcg attaaaatac cgttatttag atttacgtcg tcaagagtta
     9061 gcgcaaacat ttaaaatgag acatcaaatt acacgttcta ttcgtcaata tttggatgat
     9121 gaagggttct ttgacatcga aacaccagta ctaacgaagt caacacctga gggtgcacgt
     9181 gactatttag taccatctcg tgttcatgat ggtgaatttt atgcattacc acaatcacca
     9241 caattattta agcaattatt gatgattagt ggatttgaca aatactacca aatcgtaaaa
     9301 tgcttccgtg acgaagattt acgtgcagat cgtcaacctg aatttacaca agtcgatatt
     9361 gaaatgagtt ttgtagacca agaagatgtg atgcaaatgg gtgaagaaat gcttaaaaaa
     9421 gttgttaaag aagttaaagg cgttgaaatt aatggcgctt tcccacgcat gacatataaa
     9481 gaagcgatgc gtcgctatgg ttctgataaa ccagatacgc gttttgaaat ggaattaatt
     9541 gacgtttctc aattaggacg tgatatggac tttaaagtat ttaaagatac tgttgaaaat
     9601 gatggtgaaa ttaaagcaat tgttgctaaa ggtgcagctg aacaatatac tcgtaaagat
     9661 atggatgctt taacagaatt tgtaaacatc tatggtgcta aaggattagc gtgggttaaa
     9721 gttgtggaag atggtttgac aggtccaatt ggacgtttct ttgaaacaga aaatgttgaa
     9781 acattactta cattaactgg tgctgaagct ggtgacttag taatgtttgt tgcagacaaa
     9841 ccaaatgtcg ttgcacaaag tttaggtgca ttacgtgtca aattagctaa agaattaggt
     9901 ttaatcgatg aaacaaaatt aaacttctta tgggtgacag attggccatt attagaatat
     9961 gatgaagatg cgaaacgtta cgttgcagca catcatccat ttacatctcc aaaagaagct
    10021 gatattgcta agcttggcac tgcgccagaa gaagctgagg caaatgctta tgacatagta
    10081 ttaaatggtt atgaattagg tggcggttca atcagaattc atgatggtga gttacaagaa
    10141 aaaatgttcg aagttcttgg atttactaaa gaacaagcac aagaacagtt cggcttctta
    10201 ctagatgcat ttaaatatgg tgcaccacca catggcggta ttgcattagg attagaccga
    10261 ttagtcatgt tattgactaa tagaacaaac ttgagagata caatcgcatt ccctaaaaca
    10321 gcatctgcta catgtttatt aacgaatgcg cctggtgaag tttctgataa acaattagaa
    10381 gaactttctt tgcgaattcg tcactaaatt ttaaaaaatc aataaacttt ggtttgttat
    10441 aatttcatta attttaatta tgtggtaata taacatcata agatagtcat ctgagttgtt
    10501 cgtaagtttg ctttttaatt tgggcctaac actctttgat caagggagcc caatgggttt
    10561 tcttgcagcg tacacgcctt atatgaagga cgtacaaaac aagaaacagg gcacccacct
    10621 gtatataaca ggccgaatga tcaagctatt ataactacgg caatacggac tctatcgata
    10681 cgcaagactt tgtcttgcgt atttttatgc gtttctttaa gtttttaata aaggattagt
    10741 tgggtttaat agcgtttaat aaatgtttct tcgttgaatt tttgaaaaat aatcaaagca
    10801 ataatgctaa atataaaatt ttaagttact atgtacttat gtataaatca gtgaaagagg
    10861 taaaataatg aaacatcaat tttcaagaaa tgaactagcg attggacaac aaggtttaga
    10921 tttattgaag caacaaactg ttgtcatact tggcgttgga ggtgttggtt catttgctgc
    10981 tgaagcatta gcaagaacga atattggtca tattatatta attgataaag atgatgttga
    11041 tattacaaat gtaaacagac aattacacgc tttaactaca acggtaggac aaagtaaagt
    11101 ttctttgatg gaagaacgta ttaaattaat taatccagat tgtaaagtca cgcctttaca
    11161 tatgttttat acagaagaga catatgaaga aattttcgat aattacgata tagattattt
    11221 tgtagatgca agtgatacaa taatgtacaa agtacactta atgaaagagt gtcttgaccg
    11281 agggattaaa gtgatttcca gtatgggtgc tgcaaataaa acagatccaa cacgttttac
    11341 aatttcagat atctctaaaa cacataccga tccaatgtcc aaaattattc gtcaaaaatt
    11401 gaaaaaacta ggaattcgta aagggattcc agttgtattt tcagatgaaa gtccaattgt
    11461 cataagagaa gatgtaaaag atatagttgg agataaaaat gctatcaatc gaaaaggaca
    11521 aatgccacct tcttcaaatg cctttgtgcc aagtgttgtt ggattaattt gtgcaagtta
    11581 tgtggtgaat gacgtattaa aagatattcc agttcgtcgc attaaagaca aagggcaata
    11641 attaattttg aaagtcataa ttttcaacgt aaggcaagtg taaccgcacc ataaaactaa
    11701 aactgactag ttcgcaagca tagtagaata tgcctcgtgt actagtcagt tttgatttga
    11761 ttatagctaa aatatcaaag gtaatcgatt taatcttatt tttacggcct ttgtttaagt
    11821 aagttgttat atatttcttt aagttgttgt tcacttttag atgtcgtttt tggttcataa
    11881 taaattttgt ttttaagttt atcaggtaaa tattgctgtg aaacatagcc attaacatat
    11941 tggtgtggat atttgtaacc aatagatcgg cctagatctt tagcaccttg ataatgtcca
    12001 tcttttaaat ggtttggaat ttggcccaca tgaccgtttc taatatcgga caatgcacta
    12061 tcaatggcac tcattgctga atttgactta ggtgataagc atagttcgat tactgcttgg
    12121 cttagtggaa ttctagcttc tggtaaacct agacgttctg ctgattcaat agcagcaagt
    12181 gttctctgac cagcattagg tgaggctaag cctatatcct catagctaat tacaagtaat
    12241 cgtcgaacta ttgtaggtaa atctccagct tcaattaatc gtgctaaata atgtaaagcg
    12301 gcattgacgt cgctaccacg gatagatttt tggaaagcgc tcataacatc gtaatgcatg
    12361 tcaccatcct tatcacttac aaatgcacct ttttgtaaac agtctttagc atcttgcaat
    12421 gtaacatgtc gataaccgtc tttgtcatta tctgcgctta atacagctaa ttccaatgca
    12481 tttaacgcac tacgaacatc accttggctt tgtgtagaaa agtaggtcat ggcatcttca
    12541 tcaattttgg gttgatatgt tttcaaacca ttctcatcat cttctattgc acgagttaac
    12601 gcttggcgca catcttcgtc atttaaagga tataattcga aaatttgcgc tcttgaacga
    12661 atcgctggat tgatagcatg ataaggattt gaagttgtag caccgatcaa gacgattttg
    12721 ccattttcta aatgaggtaa taaaaagtct tgtttagctt tatctagtcg atgtatttca
    12781 tctaataaca agataacttg accagacatt ttagcttctt caacaacaag ttgcatatct
    12841 tttttagtgt tagttacagc attcaattgt ctgaatttat attgcgtact gcccgaaatt
    12901 gctttggcaa tacttgtttt gcctatacca ggtggaccat aaaaaatcat tgaagttaat
    12961 ttttttgtat caaccattct tctgataatg cctcttggtc caactaaatg ttgttgggaa
    13021 atgatttcat ctatattttt tgggcgcatt ctcgatgcta atggttctgt actcacattt
    13081 gtcacacctt tcttatacta tcttaactca aaaaatgcta aaatgggaat atcaatattc
    13141 gaattaagtg aggttataca atgaaaattt ctactaaagg gagatatgga cttacattga
    13201 tgatttctct tgctaaaaaa gaggggcaag gatgtatatc attaaagtca attgctgaag
    13261 aaaataattt gagtgattta tatttagaac agcttgtagg tcctttaaga aatgcggggt
    13321 taattcgaag tgtacgcggt gctaaaggtg gataccaatt aagagtgcca gcagaagaaa
    13381 tctcagcagg ggatattata agactgttag aaggtccaat tacatttgtt gaaagtattg
    13441 aatcagaacc acctgcgcaa aaacaactat ggattcgcat gagagatgca gtgagagatg
    13501 ttttagataa tacaacattg aaatatttag cggaatatgt agatacaagt gaagatttag
    13561 acggatacat gttttatatt taatcgtata aatactatac tataacataa aaacttcata
    13621 ttataatgtt tagcgaacct ccttagtggt atataaatat atacatccaa ggaggtttca
    13681 ctatggcaga cgaaagtaag ttcgatcaat ttaaaggaaa tgttaaagaa acagtaggta
    13741 acgttactga taacaaagaa ttagaaaaag aaggtcagca agataaagcg actggtaaag
    13801 caaaagaagt tgttgaaaat gctaaaaaca aaataactga tgcaattgat aaacttaaaa
    13861 aataagttca attaaactaa tcaaatataa gaggagcgat taatatgagt tttatggata
    13921 aagctaaaga cgcagtagaa aaatttaaaa atagcgataa tgaacaagtt aaaaatgtaa
    13981 aagataagat aaatgaatat acaggatcga ataacgaaga gaaaaaagaa aatgaagata
    14041 aagagaaata aatttcttta aattttggat atgcttttca atattttgaa agcgccccat
    14101 caaataaaca cttacacagt gtaaagttga aggggcgctt tcatatttgc attagaattt
    14161 taagtgaatt tgaatcatta ctaaaataaa tgattgaaca ataaagacat ctttcattca
    14221 aaatgctgtt actgtatatt tttattaata ttatggatac atactttcac ttagtttatt
    14281 tatcacatta taaagtgttt ttttcattta tctgatttat aacattcttg aaccgacgat
    14341 aagagtctaa ttgcatatct ttatcataaa tataacttat tgccataatt tcgttgactt
    14401 caccataagt ttccataaaa tcttgaattt tttgttgaac agtattttct gaacctatta
    14461 atgatatttt ctgtctttcc atagccatcg cgtattctct cggcgttaat atattttgta
    14521 gttcatgtgt tggcggttga acgggctgca ttctgccacg agtgatacta accattactt
    14581 gtgccataga tgaagctaaa tattgtgctt cgtcatcatt ttcagcaacg attgtattta
    14641 aacatacaat aacataaggt tcgtctaata catcagaagg ttcaaataac gttttgtaaa
    14701 tttcgatagc ttctttcatt tgttgaggtg caaaatgtcc agcgaacaca tatggaagcc
    14761 ctttgcgagc agctaaatgt gcagaatctg ttgaagaacc aagaatatat aaaggcacat
    14821 ttttacctac tgctggataa gcacgaacat atgcttgttg gtgagcggga ccgaaatatt
    14881 gttgtaataa tgaaacttct tccggaaatt tataaatacc atcatgttga tctcgtctta
    14941 atgcacttgc ggtcatcata tctgttccag gtgcacgtcc taatcctaaa tcgacacgat
    15001 ttggaaataa tgttgccatc gtgccaaatt gttccgcaac gattaatgga gcatggttag
    15061 gtaacatgat tcctccagaa cctacacgta tgtgtttcgt atgttctaac gtatgttgaa
    15121 tcaataaagc agtcgctgaa cttactaggt tgggagcgtt atggtgttca gcaatccaat
    15181 atctttcata tgataattcg tctaaatgtt gtgcgagctt aaccatatca ttaattgctg
    15241 ttcgttcatc ttcaccttct cgaataggga caaggtttaa tgctgaatat ttaatcgcca
    15301 tttttgtatc gtccttcctt ttgtatgata taagaagtat accaatcgaa taaccagata
    15361 aatattaaat tgctcataac caaaataata gtacgttaca ttttacgaat gaaaaaatat
    15421 ctgcaaatac taaaatttat aaaaaagaca agaataaagc gataaactta aaaaagccta
    15481 tcgaaaagca tgtgtagaaa atggtaaaat ataattaaaa acattataaa taaagttaaa
    15541 cagaaaagta gaaaaatagt atgtaaggtg ctattattgg aacattgata aaattgtgag
    15601 gagtgtccaa acatggaaat ttatgcagat tatgctgcga ccacaccagt aaaacctgaa
    15661 gtagtagatg cgatgatgac gatttacaat tcgcattatg gtaatccatc atcaatacat
    15721 gctaagggaa gagatgcgcg taaatattta gatgagtcaa gacgtcaaat tgcacaatta
    15781 cttggtgcag atacacatga aattatattt acgagtggtg caacagaatc gaataatacg
    15841 gcaattaaag gcatagtaaa agcaaatgaa caattgggta atcacattat cacatcgaag
    15901 attgaacatc attctgtgtt acatgttttt gaacaattag agcgagaagg ttttgatgta
    15961 acatatctag atgttgatga tacaggtgct attgatttag accaacttga agaaacaatt
    16021 acagataaga cgattttagt atctattatg tttgtaaata atgaagttgg cacagtgcaa
    16081 caaatttatg atattcaaga tattattgct gagacaaatg catatttcca tgttgatgcg
    16141 gttcaagcaa ttggacatct tgatgttaag tttgacgaat ttgagatcga cgcaatgagt
    16201 attactgctc ataagtttgg aggaccaaaa ggtgttggtg cattattagt taaagaccat
    16261 gtcacattag attatcctca acttggcggt gaacaagaat tgaaacgccg tgcaggtact
    16321 gaaaatcttg cacaaattgt cgggatggct aaagcattac aattagcaga aaagaatcga
    16381 gacgataaca atattcattt aatgaattta aaagaacaat ttttagttaa attacaagaa
    16441 agagcgattc catttgaatt aaatggttca atgacggatg ctacaggcca tattgtaaat
    16501 ctatattttc cgtttgttga agttgaaacg atgttaacat tgttggatat ggcacaaatc
    16561 tatgtgtcgt ctggatctgc atgtacagca ggctctacac aaccgtcgca tgttttagat
    16621 gcaatgttcg aagatgagga gcgatcaaat cattcgattc gatttagttt taacgaattg
    16681 actactgaaa atgaaattaa tgcaattgta gctgaaattc ataaaatata ttttaaattt
    16741 aaggaggagt cataattgtc aaataaagat atacgtgttg tcgttggtat gtcaggcggt
    16801 gtagatagtt ctgtaacagc ccacgtctta aaagaacaag gttatgatgt cattggcata
    16861 tttatgaaaa actgggatga cactgacgaa aatggcgtat gtactgcaac tgaagattac
    16921 aacgatgtta ttgaagtgtg taatcaaatt ggcattccat attacgctgt taattttgaa
    16981 aaagaatatt gggataaagt ctttacgtat ttcttagatg aatataaaaa aggtcgtact
    17041 ccaaacccag acgttatgtg taataaagaa attaagttta aagccttttt agatcatgcg
    17101 atgaatttag gtgcagatta tgtagcaaca gggcattacg cacgcataca tcgtcatgaa
    17161 gatggtcatg ttgaaatgtt acgtggtgta gataataata aagatcaaac atacttcttg
    17221 aatcaattat cccaacaaca actttcaaaa gtgatgttcc caattggcga catcgaaaag
    17281 agtgaagtgc gtcgaattgc tgaagaacaa ggacttgtta ctgctaagaa aaaagattct
    17341 acaggcattt gttttatcgg cgaaaaaaac tttaaaacat ttttatcaca atatttacct
    17401 tcacaaccgg gtgatatgat aacacttgat ggtaagaaaa tgggtaaaca tagtggtttg
    17461 atgtattaca caataggaca aagacatgga ttaggtatag gtggagatgg cgatccttgg
    17521 tttgttgtcg gtaaaaacct aaaagataat gttttatatg tagaacaagg attccatcac
    17581 gatgcattat acagtgatta cttaattgct tcagactatt catttgtaaa tccagaagat
    17641 aatgacttag atcaaggttt tgaatgtaca gctaaattta gatatcgcca aaaagatacg
    17701 aaagtttttg tgaaacgtga aaacgatcat gcactacgtg ttacttttgc tgagccagta
    17761 agagcaatca cacctggaca agcagttgtt ttttatcaag gtgatgtgtg tcttggtggt
    17821 gcaacaattg acgatgtgtt caaaaatgaa ggtcaattaa attatgttgt ataaacaatg
    17881 gcaacaataa attacttatt tgaagtttca acgtcgaaaa tgacgaaaga cagtttttga
    17941 tgagaataat tcatgatgat agagtctggg acatcacaat gtcctaggct ctacaatgtt
    18001 atattggcgg ggccccaaca tagagaattt cgtaaagaaa ttctacaggc aatggaagtt
    18061 ggggacgggg ccccaacata gaagctgacg gaaagtcaga tttcaataat
//
LOCUS       DAHHYJ010000045        17992 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000045 DAHHYJ010000000
VERSION     DAHHYJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17992
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.045"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(350..1729)
                     /locus_tag="NBK22_001133"
     CDS             complement(350..1729)
                     /locus_tag="NBK22_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2414328.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGVIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EEVEGHPFKMPGGAVSNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            2009..2365
                     /locus_tag="NBK22_001134"
     CDS             2009..2365
                     /locus_tag="NBK22_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414329.1"
                     /translation="MLKQILPRATKISILFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LAFILICLTLFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            2362..2547
                     /locus_tag="NBK22_001135"
     CDS             2362..2547
                     /locus_tag="NBK22_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414330.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(2643..2834)
                     /locus_tag="NBK22_001136"
     CDS             complement(2643..2834)
                     /locus_tag="NBK22_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414331.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFRVQVRSFVVGS
                     MIYLILATIGYFLLLQGKL"
     gene            complement(2845..3480)
                     /locus_tag="NBK22_001137"
     CDS             complement(2845..3480)
                     /locus_tag="NBK22_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD2414332.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            complement(3555..4472)
                     /locus_tag="NBK22_001138"
     CDS             complement(3555..4472)
                     /locus_tag="NBK22_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCD2414333.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            4740..5270
                     /locus_tag="NBK22_001139"
     CDS             4740..5270
                     /locus_tag="NBK22_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414334.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            complement(5336..6940)
                     /locus_tag="NBK22_001140"
     CDS             complement(5336..6940)
                     /locus_tag="NBK22_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2414335.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            7270..8034
                     /locus_tag="NBK22_001141"
     CDS             7270..8034
                     /locus_tag="NBK22_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD2414336.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTKKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            complement(8092..8598)
                     /locus_tag="NBK22_001142"
     CDS             complement(8092..8598)
                     /locus_tag="NBK22_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCD2414337.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(8765..10078)
                     /locus_tag="NBK22_001143"
     CDS             complement(8765..10078)
                     /locus_tag="NBK22_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCD2414338.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSLGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLIAILFNFPKLKKNTSQEMKH"
     regulatory      complement(10155..10250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(10303..10872)
                     /locus_tag="NBK22_001144"
     CDS             complement(10303..10872)
                     /locus_tag="NBK22_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414339.1"
                     /translation="MALIVSRAILFLILALAFGIATVFPEQKLPLYIVFTLFVAVAIV
                     SNVWFTHQNVKKHIIKFNKYVAYFLSFVSGLLYIGFVIYTLTIGSYDVLPIIIFAISI
                     VLVQILENILGISIKNTRLSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLKW
                     WLLLVISIIVLIIITVAMIVYEKYYTTPD"
     gene            complement(11359..12240)
                     /locus_tag="NBK22_001145"
     CDS             complement(11359..12240)
                     /locus_tag="NBK22_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2414340.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLQGYKILVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEQAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            complement(12351..13472)
                     /locus_tag="NBK22_001146"
     CDS             complement(12351..13472)
                     /locus_tag="NBK22_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCD2414341.1"
                     /translation="MGLKDQAVTWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGLSCIAVRADIDALPIQELVEQDFKSENEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQSAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQDIADGLAQAYNMK
                     INLNYTHLPGAVMNDEALTHKAIAVAQHVGYKVEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            complement(13749..14987)
                     /locus_tag="NBK22_001147"
     CDS             complement(13749..14987)
                     /locus_tag="NBK22_001147"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCD2414342.1"
                     /translation="MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKDGKVIVDNTVSFKA"
     gene            complement(14987..16648)
                     /gene="hutU"
                     /locus_tag="NBK22_001148"
     CDS             complement(14987..16648)
                     /gene="hutU"
                     /locus_tag="NBK22_001148"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCD2414343.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            16808..17692
                     /locus_tag="NBK22_001149"
     CDS             16808..17692
                     /locus_tag="NBK22_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2414344.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
BASE COUNT         5908 a         3611 c         2526 g         5947 t
ORIGIN      
        1 atttatgcat cattttcaaa acttacatgt cttttatctt gtataacata cgcatatgac
       61 caatgatttg ataaggccat tctatttctt atattaaaaa caaacgcagt tgattaacgg
      121 attcattcca tatatcaatt gcgtttgtta tttttataat gctatttttt attttaaagc
      181 ccacagtaaa aactaacgat aatattgcca cttataagtt tttcacacta caacttcgat
      241 ttaagctata tttataaaaa aatgcgccta ctcataacat tctttggcta tttaacttta
      301 ataagctagg gccaagtttg tcataaaata agcgcacatt ataatcatac tattttgctt
      361 ttacattatt tttattatat ctaataaaat aataaatcgt tacaatcgtt aagaagatga
      421 caccaataag cactgatatt ctagtttcaa cattaaatac cataccgact aatactaata
      481 ttaagaataa tattgttaag tagttactta ctgcgccacc aggcattttg aatgggtgac
      541 cttccacttc ttcagggtgt aattttctaa aacgtaagtg actgaataaa atcataaacc
      601 aagggatcat acctggtaaa atagacgcac tatatacgta tacgaaaata ctcttcgctc
      661 catcgatata taaaggtaaa attacattta ataaagcacc aattaaaata ccaagagata
      721 ctgcaacaac agtgtacaat ggcacaccat ttctcatgat tttagtaaat attttcggca
      781 tttcaccttt atgagcaagt gtataaatca tacgactcgc actgaaaata cctgagttac
      841 atcctgacat tgctgcagtt aatacaacaa agttaattaa tccagctgca aatgtaatac
      901 cgattttagc aaatgttgca acaaacggac ttccaatgtc gcctaattga ttccaaggat
      961 acactgaaac aataacaaaa atagcaccta aatagaaaat taaaattctc cagataacac
     1021 cattcactgc tttaacgata tttttctgag gatctttagt ttcacctgca gtaataccta
     1081 tcagttccac accttggtat gaaccaatta caattgataa tgcaaagaag aaaccaacaa
     1141 taccatttgg cataaatccg ccatttgtcc atagattaga aatacctacc gcatggccac
     1201 catttccaaa accaaagaaa ataagaccaa aaccagcaat aatcattaaa ataattgtta
     1261 caactttaat taaagcaaac caaaattcaa attctccaaa cgcttttacc gagaataaat
     1321 tcgctgccat taataataaa atagcaataa caccaggaat ccaagttggc aattccggga
     1381 accaaaactt catatattct cctactgcga tgacctcact cataccaact acaatccatt
     1441 ggaatatatt actccatgct gtcatataac ctgctgcagg atgtatataa tcacttgcaa
     1501 atgttgcaaa tgatcctgtt gtagggttta aataaatcat ttcccccatt gctctcatga
     1561 ttaaaaataa aaagataccc gcaattaaat atgcaaggat aactgatggg cctgtccatt
     1621 taattgtact tgtcgcaccc atgaaaagcc ccacgccaat cgtaccacca agtgcaatca
     1681 tttgaatttg acgggcactc aatccccttt gtagttcatt gttctccata acttcttacc
     1741 ccatctctga ttgatactaa ataaatccat aaaaaaactt attgccgtaa ttatactata
     1801 aattatcgat tttggtacat taatatttgc ataccatgca atcgtttaca gaactactat
     1861 agcaggcttt cgcaactttg caaacaatta ttttgtgatt tttctaaaaa attaaaaaag
     1921 ttgtccgtga accgttgagt cacatacaac ttcagctatt ctcggtaatt atgatataat
     1981 tttaataata ttaaaaagga gtatatcaat gttaaaacaa atactgccac gtgctacaaa
     2041 aatatcgata ctctttgctg tagcattttt catcataaat tatatcggta tggaaaaacc
     2101 tgacatccta tatttagttg gaagaactat tattgcaaca cttgctttca tactcatttg
     2161 tcttacatta tttactatta ttaattctcc agagcgcaag attaaactcg gtacgacttt
     2221 accgattgca ttaattattg gtatcatttt cggcgccata tttttaacgg tacaaatcgg
     2281 tgtcatcact gggttaatca tcggtgtgat tgctacgttt atttgggaat tgattgaaaa
     2341 aaataaagga ggtcgttcat catgacaaca gcattaattg gtgtaattgt attaatctta
     2401 ctaattatta gctttgtgcc aaactaccgt gctatgaaat cagcaaaaga acaaggaagt
     2461 aatgcaacaa gagctacaat tatggtaggc atagatatta tcttaattgt acttatcatc
     2521 gtgacgttat tattaaaata tatgtaacta tttaaaccac cctatctcat acataaacat
     2581 gaacgttctg ccatttcaca tacatatggt cgtccattta atgaggtaag gtggttttct
     2641 cgttaaagtt taccttgtaa tagtaaaaaa tagccaattg ttgcgagtat taagtaaatc
     2701 attgaaccaa caacaaaaga tcttacttgc acgcgaaatg gtgtctgttt gttaaatata
     2761 ggaccaatca ccaaatttcc aacgcctaaa attactggac cagctagaaa aaacaatata
     2821 atgtaaccca tcatgatacg cctcctattt cgtaaagaaa cgcgctacaa tttgatcgta
     2881 atcaatatct tttgctacat aatctacatt agaaaagtcc tgtgcgcttg tcattttatt
     2941 cgtattaaca ataacatcta atccagctgc aatcgcggct gttgcaccat ttacagaatc
     3001 ttcaatagcc aaacattgtg tcggcatata atttaattgt tgtactgcag ataaatataa
     3061 ttcagggtca ggtttaactt gttcaacatc ttctctacca acaactacct ctatataatc
     3121 gtctagacct aatgctttaa aggtgggcat tatttccgct ttcacactac ttgtggcaat
     3181 tgccatagga atgtgacgtt gctttaaaaa tgccatcaat gatttaatcg tatcaatcat
     3241 cggtaatgtt gtactagtaa gatgatgttc ttcataaagt ttatctttat tttccgaacc
     3301 aatcgcttta attaaatggt catgcaaatc tgtagctgct cctccaatag aagaacgata
     3361 aaaatcaatg cttataggat cggcattatg catctctaaa tgtttattaa taacattaaa
     3421 taaatgttgt tccgtatcta ttattgttcc atcgaaatca aatataactg ctctatacat
     3481 atatttactc ctctattttt tctctttatc aaagcttaac atagtccgat gaccacttca
     3541 ttctattagt gcaattataa cttttttgac caatactttg ctaaaatagg ccctgttata
     3601 ttatgaatga aactaaacac tgcgcctggt actgctgcaa ttggattaaa atgcaatgct
     3661 gctaatgaca cagctaaacc agagttctgc attccaactt caatactgac tgctttttga
     3721 tcttgtcgat ctaattttaa aagacgagct aaccaatatc caatcgtata ccctaatacg
     3781 ttatgtaaaa taactactaa aaatattaat aatcctgtag ttaagatttg gtgcttactg
     3841 ccacctacaa ctattgctaa aattaatgaa atagcaacaa ctgatataat cggcaaagct
     3901 gtagaagctt tttcagcaat tttacgatta ataatttgca aaacaatacc taaagcaatt
     3961 ggaattaata caacttgaac aactgaccac aacatactca ggaaagatac ttccaaccat
     4021 tcatttgcaa atagatatat taacgcaggt gtaacgaatg gcgctagcaa cgtagagacc
     4081 gtcgtaatag aaacagaaag tgctacgtta gctttggcta aataactcat tacattactt
     4141 gatgtcccac ccggacaaca tccaactaat attacgccaa cagcaatatc aggtggtaaa
     4201 tgaaaagact ttgcaattat aaatgctaat gtgggcataa tactgaattg tagacagaca
     4261 ccaataatta ctgctctagg tgctttaaag accattttga aatcattagg tgtaattgtt
     4321 aatcccatac ctaacataac aataccaagt aaataaggta cccatttacc taatgtcgca
     4381 acatgttgtg ggaaaataaa tccaataaca gctgctacta acatccatac caaaaaactc
     4441 ttcgtggcaa atatactcaa tttttgaaac attgttaaca catcctcgtc gataatattg
     4501 aaaaatatta ccataagacc agtaaggtta caattacttt tctgaaaatt aagattattt
     4561 agtcgttctc ttcttattgc aaatatttta tcactgataa acaattattt taaacttcat
     4621 tcaaaagtct tagtgaataa agaaaaaaag cgtggtaaaa tcaacttgaa caaaattaag
     4681 tcacctttat aaatattaaa cgtgtattta atgccatgta ttcctcggga ggtcctcaca
     4741 tgttaattga taaattcgaa acatacatca tcaacatcgc cggtttaaat gaccgtacta
     4801 ctagaaaaaa attaagtaag ttatgcaaaa gtgtacaatt ttgtgatgcg ttgcaattct
     4861 caataaacaa acagtttaat cagtatgtat tagaaattag cttaccaaag caacaacttc
     4921 catacttcat ttcattttta agttttcatc agtatagtat tttccaagta ctttcaccaa
     4981 agaaaataaa tgaattactt gattctgata atttatatca atctgcaaag cgtttcgata
     5041 tcaatattga tggcttgcaa gatgctttca ttaaagacaa agtcatcgat attatgaata
     5101 tgttccaaaa tcacactgat atcacttata cgttgaataa gtctcatgca catattatat
     5161 gtacgccaga aatatttgct aaattgttac atacgattgc aactagaaat atcgacattc
     5221 tatctgccaa ttatagatcg tcatctatga gcaaagctcg tatatcataa caaaagacac
     5281 atctatacga tgatcattta caatgattgc gtatagtgat gtgtcttttt ttgtattatt
     5341 tatcttcgga cgattgatcc tcaaccattt gttcaaatgc ttcacgaact tgtggtactg
     5401 tcatacctac tacaacttgg atactcttac cactctttac taatccgtgt gccatttgtt
     5461 gatgcgtgaa atattccgta tccgcaactt tactttcatc ataaacagtt aagcgtaatc
     5521 ttgttgtaca gttagtaaca tctttgatat tttctttgcc gcctaaaccg tcgaggtaat
     5581 acgcagcttt gtcttcgtat tcattacctg ttgaagcacg tttagaatct acgctatcac
     5641 cttttttatt tttgtaatct tgcttagaaa ataatttaac ttcctcttct gttttcttac
     5701 gtccaggtaa tggaatatca aatttcaaaa ttaagaatct gaataagaag aagtaaatcg
     5761 ctacaaatat cagaccgata ataacttgtg caacataagt accccaatgt tctttaccta
     5821 atggcaacca gtttgttgaa ataaagtcta acaagccacc acccatgtta cctacaacac
     5881 caaatgcgta cattaaagta tccattgatg ctgctaaaac tgcgtgtaaa acaaataagt
     5941 atggtgcgat gaataagaat gtaaattcta atggctcagt aatacctaca acgactgctg
     6001 ttaatgttgc aggaataagt aatgctgcta cttttttacg attttcttta ggtgtcgtag
     6061 catacattgc taaagcaata cctaaacatc caaatacttt cccatttcct tgaagcataa
     6121 agccataagg gaattgctct tttaatggct tagtactctt agcaaactca tttaaatgtt
     6181 gtaaccattc agctttcaag ccgtgattga ctactactgg tccaacttca attggtgcat
     6241 aaataaagtg atgtaatcct gttggtatta atacacgttc taagaagtgg tacagccaaa
     6301 caccgatata gcctgaagcg atgataaagt gttgcattga accaataccg tgctgaacag
     6361 ttggccaaat aacacaagta attgctgcta atggtaacat cacaaaaaat gaaattgtaa
     6421 ccacaaatgt taaaccttgg aatacaccaa ccatttctgg taatcgttta ctatagtatc
     6481 tattgtgaat ccaagtaata atagctgaaa taataatacc acctagaata ttcgtatcca
     6541 atgtggcaat acctgcaatt gattttaaac caggtacatt ttcaacgcct ttttctaaat
     6601 tagcgccaaa cgtatgtggc cattgcgtta aaatggcatt tataaatgta ttaaacatta
     6661 agtaacccat caatgctgca agtgctgcat gacctggtgc ttttttagct aaagaaagtg
     6721 gtaaacctac aacaaataca acttccatat gcgtaaaaat aacccagcca ccagattcta
     6781 tcactgacca aaatttaaac caaaacgtat gttgatctgc taaactcccc atgattgtag
     6841 gatttttaaa taatgtcgca aagccaagca ctatcccaaa gaaagcgaac attaataccg
     6901 gtacaatcat tgcactaccg aaacgcttta tcgcattcat cttctattcc ctccatatca
     6961 tctttcctaa caatacatct aattagattc atttataaat agatgtctta ctatttaaat
     7021 ataatatata gtaaacgctt acacacctac aacgacattg acgtattttg aaagtatttt
     7081 gtataatcag attatctttt catatagtga aaattttttc acgaccttat atatgacatc
     7141 gttgtatttg taatacattc gttttaaacg cataatcaaa cctatatcaa tacacatata
     7201 gatataatga catacaagat tttaatgtaa taacgatcta tcacacattt attttcaagg
     7261 aggttgaata tgtttttaga tgaacacatt aatcgaaact ttgataaact taatgataat
     7321 gatttacata tcgctcactt tatcaataca catatagatg aatgtaaaaa tatgaaaata
     7381 caagatttag cgcaattcac acatgcctcg aatgcaacca ttcacagatt tacacgcaaa
     7441 ttaggttttg acggttatag tgattttaaa tcgtacctta aatttgaaag taataagaca
     7501 caccaattac cttctaactc tatcgatagt ttcaaacaag aaatcgatag tacattcagt
     7561 tatttagaac gcgttgatta ccagcttctt actaaaaaaa tgcatacagc ttcaacaata
     7621 tatttgtatg gtacagggcg tgcacagtta aacgttgctt cagaggcaca gcgtatattg
     7681 ttaacgttac ataatcatgt cattgtactt catgatgaac atgaacttaa aatgattttg
     7741 aacaagagtg aacctaatga tttattcttt attatttcat tatcaggtga aacacatgaa
     7801 ttattagaag taacaaactt gcttcaactt agacaaaaat actttatttc tgttactaca
     7861 atgaaagata atacccttgc tcaaaaagca aattacaacg tctatgtatc cagtaacaca
     7921 ttctacttaa ctgatggtac agactattct agcttcatca gttatcatat attctttgaa
     7981 acacttgtaa gaaaatataa tgagtattta ctacataatg atatttcaaa ttagaaaaaa
     8041 ggaagttatg atttaatggc cttgagccta tcataacttc cttttatcat tttattgttg
     8101 tgttgatgtt tcgataacgt ggtacatctt atcaaacatc aattcgaaac catgcaccat
     8161 ggcatcatga tattcttttt tcttttgctt gtattctaaa ttagtaaatc gtctttcttt
     8221 ttcaactaat gaacgataat aaaatagcat ttgggtgcca cctgtttcac gttcaaaaaa
     8281 ttctacctca atgacatctt gtgtttcact tagtccaggc ataccgatag tcatcttaac
     8341 gtattcatcc ataactaaag attcataaat gccttcaatc acatttactt tgccattacg
     8401 ttgttgatct acaatacgat atttaccgcc ttctttaacg tccgcttcaa tctctttatt
     8461 cgttctggct gatgtcataa accattgttt caacaaatct ttctttgtcc aagcttcgta
     8521 tactaactct ggagaaaatt tataaagctt ttcaatttca acttcgacat gttcattctc
     8581 tacattaaat tttgccactg ttgtccaccc actttcgctc ttacttttat tttaacgtat
     8641 ttttgctcag ttccaaacat agatgatcat catttttaaa agattagcgt tatacggtga
     8701 gtacaacatg atctgttaat ataacaagcc accttacttg gctacatcga tatattgtta
     8761 agcattaatg tttcatttct tgactagtgt tcttttttag ctttggaaaa ttaaataaaa
     8821 tcgcaataag tccgcataca cctaataata taggataaat gctgtatggg aataacatta
     8881 acggtgaaat accagctaca ccagccgctg aaatgacttg cgggctatat ggtaataaac
     8941 cttggaagca gcctccaaat atatcaagaa tacttgctga tttccttgaa tctacatcat
     9001 attcatctgc aatattttta gctaaaggac ctgacataat aatagagatg gtgttgtttg
     9061 ccgtggcaat atctgcgaca cttaccaaac tagcaattcc taattctgcg ccacgctttg
     9121 atttcacttt agagcgaaca aattgcaaca accattcaat accaccattg tgttgaataa
     9181 taccgactaa accaccaatt agcaacgcaa tcatagcaat atcttccatg cttataatac
     9241 ctttggacac tgcatctagt agccccatcc aacccaatga accatctatg agaccaatga
     9301 ttccggctaa taatgttccg ccaatcaata cgataatgac atttacacct aataatgcta
     9361 ataccaatac taaaatatac ggtacaactt taattagatt ataatcatag tttttagcat
     9421 gatttaaaga aatgccattc gttaagaaat acagaataat aatcgttaaa atagcacctg
     9481 gcaatacaat tttaaagttt actctgaatt tatctttcat tttcgtatgt tgtgttctaa
     9541 ccgcagcaat tgttgtatct gaaatcattg atagattatc gccgaacatt gcacctccaa
     9601 caactgtagc cattgctagc gcagttggta catctgtcgc ttgtgcaaat ccgtaaccaa
     9661 caggtgctat tgcagcaaca gttcctacag acgtccccat agatatagaa acaaacatac
     9721 aaatcacaaa caatcctaca ataattaaat tttctgggat taatgatagt cctaaattaa
     9781 ctgtcgactt tacgccaccc attttttcag ctgtatttga aaatgcacct gctaaaataa
     9841 aaatcaacat cattaaaata atgtttgaat ggcctgcacc tttcgtgaag acctcaactt
     9901 ttttagcaaa tgattctttt cgattcatta ataacgccac aattaccgtt atcgtaattg
     9961 caacatttaa tggcattgaa gtaaaatcac ctgtgataat acctacgcct aaaaacaacg
    10021 ccacaaataa taacaagggg aataatgccc aagcattgct ctttttatgt acttccatcc
    10081 tttttacctg ctttccaatt aaaaatacct ctttctcaca aacgatgaag aaagaggttt
    10141 tcatgtgctt tacctgctta tcttcaaacc attacggtta ctggaattgg cacattcgag
    10201 atgttgccga ggcttcatag ggccagtccc tccacctctc tagataagtg atgcttattt
    10261 acgtttacgt tacaagataa tccttagtac gtcaatcata aattaatcag gagtcgtata
    10321 atatttttca taaacaatca ttgctactgt aataataatc aaaacaataa tgctaataac
    10381 aagtaaaagc caccatttaa gcattaatgc aataaaaatg aacacgatag acacacttac
    10441 taatattaat gatatgactt taaattgctg aacacgttgc ttggagatga ctttcaactg
    10501 tttgtttgat agacgcgtat tttttatact gattcccagt atattttcta atatttgaac
    10561 caatacgata cttattgcaa atataataat tggtaaaaca tcatagctcc ctatagttaa
    10621 tgtataaatt acaaatccaa tgtaaagtaa ccctgagaca aaggataaaa agtatgcgac
    10681 gtatttgtta aacttaatga tatgcttttt aacgttttga tgtgtaaacc atacattcga
    10741 aacgatcgca actgctacaa ataatgtgaa tactatatat aatggtaatt tttgttcagg
    10801 aaaaacagtc gctattccaa aagctaatgc taaaatcaaa aataatatag ctctagatac
    10861 tattaatgcc ataataacaa cccctttgtt taatatcgag tttgcaaatt tacgtttatc
    10921 agcgtttcta tgatcagtac ttctacgggt agcgtttcta tgtaatttac atcatcttaa
    10981 catataaata cttcgctatt taattgaaaa catatcctat tattctttgt ccgttctgac
    11041 gtttaatatc tagccttagg catttcactt gttaatgaat ttaactttct tccactaacc
    11101 gtccctaaac ccaatcccgc aacagttttt aactttttcg ttgttgtcct gacatcctca
    11161 ttaagaaagt ttattctgct taaaacttat aatccacacc ctgagcaaac gctccttatg
    11221 acagagtatt aaaataagcc gataaagata cacaccttta ccgactattt aaaatacact
    11281 tcaccaattc attttaattt aatggattga agtaactaaa ttaatattat gttgttcaat
    11341 taaaagcttc atacaaacct aatctatttg cactccaccg ctaacaccga acacttgtcc
    11401 ggttgtataa cttgattctt ctgacgctaa tagcacataa gttccacata actcaacagg
    11461 ttgacctgca cgacctaaag gtgttttttg accaaatgtt gggattttac tttgaggttg
    11521 tccaccagaa atttgtaatg gtgaccagaa tggaccaggc gctacacagt tcactctaat
    11581 tccttttggt cctaattctt ctgaaaaact tttagttaat gaaataattg ctgcttttga
    11641 agcggcataa tcatgaagaa taggactagg attataacct tgtacagatg atgtcgttgt
    11701 aattgacgca cccggtttta aatattccaa tgctttttga actgtccaaa atagcggata
    11761 gacattcgtt tcaaatgttt ctgtaaatgc ctcagttgta aatccatgaa tatcatcatg
    11821 atactgttga tgtccagcaa ctaaagtaac attatctaag ccacctaatt gttgatatgc
    11881 ttgttcaaca aggtcatagt tgaactgttc atctcttata tcaccaggaa ttaacactgc
    11941 cttttgacca ctttcttcaa tcacttggcg tacttcttgt gcatcttgtt cttcactcgg
    12001 aagatagtta atcgctacat ctgcaccttc tttagcatac gcaattgctg ctgcacgccc
    12061 tattgctgag tcaccacctg tgactaatat tttatagcct tgtaagcgtt gatgaccttg
    12121 gtaagacgtt tcgccacaat cgggtgctgg cgtcatttca gattgtaaac ccggtacctc
    12181 ttgttcttgt ttttcataat ccgttgtttt aaattttgtt ctaggatctt gagctgccat
    12241 ttttttacat ctccttattc gcttaatggt tattatttac ccaatcttcc taggaactta
    12301 atcatgatta cactaaaaat tactttcttc tttataaaaa caagctcgaa ttattcatgc
    12361 aatagtctct ttacaaattc aacaaaatac tcaggtactt tttccagaat cctttcatcc
    12421 ggtttatatt gaggatgatg taaatcatat tcactatgag aaccaattaa cgcaaataca
    12481 cttggaaaat gttgactata acctgaaaaa tcttctccaa tcgtaagcgg ctgttccatc
    12541 atttccacct tatatccaac atgttgggct actgcaattg ctttatgcgt caatgcctca
    12601 tcattcatca cagcgccagg taaatgcgta taatttaaat taattttcat attatatgct
    12661 tgagccaatc cgtccgcaat atcttgtaat cgtgtttcta caagctttcg taccacagga
    12721 tcaaaactac gcactgtgcc ttgtacatac gcatgatcag caatgacatt ccaagtatta
    12781 ccacatgata tttgtccaat tgttactacc gcttcatcaa acgcagatag atttctacta
    12841 actatggatt gaatactatt aatcaattgc gccaacacaa taactggatc gttgcattgt
    12901 tctggctttg cagcatgacc acccacgcct ttaatatgaa actcaaaacg atctactgct
    12961 gatgtaattg cccctgtttt gattgcaaat gtacctaccg aacgcgatgg gtcattatga
    13021 aaacccaata ctgcttgtac atcttttaat gcatgtgttt caataatttt aaaagcgcca
    13081 tgtcctagtt cttctgctga ttgaaaaatg aatttaacac gcccagtaag agtgccctca
    13141 atttctttta attttacagc tgtagccaaa atactagcca tgtgaatatc atgaccacac
    13201 gcatgcataa caccttcatt ttcagattta aaatcctgtt caacaagctc ttgaatcggt
    13261 aaagcatcaa tgtcagcccg aacagctata caactcagac cttgtcccac ttcagcaaca
    13321 agcccagtcg caagtggtaa gtctaatatt ctaatatgat gttctgttaa aatatcttta
    13381 attttttgtg tagtcttaaa ttctttatcg gatagttctg gaaattgatg aaaatacctt
    13441 ctccaggtaa cagcttgatc ttttaatccc atcggtcatt ccccttcctt aagtcaatga
    13501 tatgttgtct accctacgat gatcatcttt gactattaaa cgatgatttc acaacaatgt
    13561 actcttgtta attgctttcg ttaatgatag acagttgttt aataatatcg taacactgtt
    13621 gtcaaactat tctaactttt ataattgaga ctctatacaa aaacgtgttc tcgaatatac
    13681 ttgtttttac aaaccacaaa aagctctaaa cattagttta aaccaatgct tagagctttc
    13741 taattatttt atgctttaaa agatactgtg ttatctacga tgaccttacc gtctttaata
    13801 actttttctg cgtgattgat accaaaatga tatggaatat attcatgatt tggtgcatcc
    13861 caaattacta aattagcctt atcacctgtg ttaattgtac ccgcgttaat gtctattgct
    13921 ttagcagcat tgaccgtaac agcattccaa acttcattag gtgatagctt taatttcaag
    13981 gctgcaatcg ccataacaag ttgtaagttg tttgtgacac tactaccagg gttataatca
    14041 gttgctaatg caatcgcacc gttattgtca agcatgcccc ttgcatctgc ataatcttct
    14101 ttacctaaat agaacgtcgt tgcaggtaag aggacagcta cagtatcact atttcgcaac
    14161 ttttcttttc ctttatcact agaagctact aagtggtctg ctgatattgc ttgttcatca
    14221 attgctaatt ccagtccgcc taacggatca atttcatccg catgtatttt cactttaaaa
    14281 cctgcttctt tggctttttg catataatgt tgcgattgtt ctattgtaaa tacacctgtt
    14341 tcacagaaaa tatccgcaaa gtctgcatat tgttttactt ccggaagtaa cgcaatcatt
    14401 tcttctaaaa atgcctcatt tgaacttgcc tctttaggta cagcatgagg ccctaggaaa
    14461 gtatgtttca tgtctaaatc atatttctca gctaaacgat tagacacttt caattgcttc
    14521 agttcatttt ctctatctaa tccataacca ctcttacttt caactgcaag cacgccgtgt
    14581 ttaatcatag taagcaaatc atgctctgct tttttaaaca agtcatcttc ggatgtttct
    14641 ctagtagcat taacggtaga taatatgcca ccacccattt ctaatatttc aaggtaagac
    14701 ttaccttgac gttttaatga catctcatgt tctcgagatc caccaaatgt taaatgggta
    14761 tgtgcatcta ctaatgctgg ggacactacc ttcccactag catcaatcgt ctcagtcgca
    14821 tcgtagtcat ctgtatgtgt tccagcatat acaattttgc catctttaat gacaactgta
    14881 ccatttttca caacatttaa ttcatctaat tccttaccct tcaaaggttt atctgttgat
    14941 ctcggtaaaa ttaattctgc tatatgatta attattaaat cattcattac ttatcacctg
    15001 ctttatcaat cattggaata tgaatacctt tttctttagc tgtttgaata gcgatgtcat
    15061 agccagcatc aacatgtcgg gcaacaccca tacctgggtc agtcgtcaat acacgttcca
    15121 atcttctttc agcacgctct gatccatctg ctacaacaac catacccgca tgaagtgaat
    15181 atcccatgcc aacaccgcca ccgtgatgga atgaaatcca tgaaccacct gcagctgtgt
    15241 taatgagtgc attcaataca gcccaatcac caaccgcgtc actaccatct ttcatacttt
    15301 ctgtttcacg gttaggacta gcaactgaac cagcatctaa atggtctcgt ccaataacaa
    15361 ttggtgctga aatttcaccg tcacgtacaa gacgatttaa agctaagccc attttcgctc
    15421 tttctccata gcctaaccaa gcaatacgtg atggtagtcc ttgatatgaa attttttctt
    15481 cagctaaatc aagccatctt aataactttt cattttctgg gaaaagtttg cgcatttctt
    15541 catccgcacg ctcgatatct tttggatcac cactcaacgc agcaaagcgg aatggccctt
    15601 taccttcaca gaataatggt ctaatgtaag ctggtacaaa gcctgggaag tcaaaagcat
    15661 ttttcactcc gttattgaag gctacttgac gaatattgtt accataatca aatgctacag
    15721 cgccacgttt ttggaattca agcattaatt caacatgctt tgccattgaa gcttgtgaca
    15781 gttcaacata ttttttcgga tctttttcac gcaatacttt cgcttcttct acagagtatc
    15841 cttgtggcac atatccattt agcggatcat gtgcacttgt ttggtcagta ataatgtcaa
    15901 ttttaaatcc tttttctaga atcgcttgat ggatgtctac agcatttcca actaacccga
    15961 ttgataatcc ttctccacgt tctttcgcct cttctgctaa ttttaatgct tcatctaaat
    16021 cagctgtttt aacatcacag tatttcgtat caattcgctt atcaacacgt gtttcatcaa
    16081 catccacgca aattgctacc ccatgattca tagtaattgc taacggttgc gcaccaccca
    16141 taccacctaa acctgctgtc agtgtaacag tgcctgctaa atctccatta aagtgttgat
    16201 tacctagctc ggcaaatgtc tcataagtac cttgcacaat accttgagaa ccaatatata
    16261 tccaactacc ggctgtcatc tgtccataca tgattaaacc ttttttatct aattcattaa
    16321 aatgatccca gtttgcccat tcaggcacta atactgaatt tgaaattaat acacgtggcg
    16381 cttcttcatg tgttttaaat acagcaactg gctttcctga ttgtactaac attgtctcat
    16441 ctgattctaa ttctcgtaac gttttctcta ttgcttcaaa agcttcccaa ttacgtgctg
    16501 cttttccaat accaccataa acaactaaat cttctggtct ttcagcaact tctgggtcta
    16561 aattgttgta taacattcta agtactgctt cttgttccca acctttacac tcaatactca
    16621 aacctttttt tgcttgaatt tttctcataa aattcgctcc tgttctttta agaagttaat
    16681 tccactaaat ttaaaacgct tacattatta tcttcaatat tcattatagt atgttaaaat
    16741 atagccaaca aatataaata aactaattat ccatagcttg aatctataaa taaaaggagc
    16801 aaaacacatg aaaattattc agttagaata cttcttggct atcgtgaaat ataatagttt
    16861 tactaaagct gcacaatttt tacatattag ccagccatct ttaactgcta cgattaaaaa
    16921 aatggaagca gatttaggtt atgacttatt tacacgttca acaaaagaca tcaagattac
    16981 cgaaaaagga atacagtttt atcgttatgc gagcgaatta gttcaacaat atcgatccac
    17041 gatggaaaaa atgtatgatt taagcgttac atcagaacca aggataaaaa ttgggactct
    17101 tgaatctacg aatcaatgga ttgcgaattt aattcgaaag caccattccg actaccctga
    17161 acagcaatat cgtttatatg aaatacatga taaacatcaa tctatagagc aattactgaa
    17221 ttttaatatt catttagcta taacaaatga aaaaataacc cacgaagata taagatccat
    17281 tcctttatat gaggaatctt acattttatt agcacccaag gaaacattta aaaatcaaaa
    17341 ttgggtagat gttgaaaatt tgccactcat attaccaaac aaaaattctc aagtgcgcaa
    17401 acacttagat gactatttta atagaagaaa tattcgtcca aatgtcgttg tagaaacaga
    17461 tcgattcgaa tcagcagttg gatttgttca tctcggctta ggttacgcta tcattccgag
    17521 attttattac caatcatttc acacgtctaa tttagaatat aaaaaaattc gtccaaactt
    17581 aggccgaaaa atttatatca attaccataa aaaacgcaaa cactccgaac aagtacatac
    17641 attcgtacaa caatgccaag attatttata tggactttta gaggctcttt aacttaagtt
    17701 attagagcct cttatgcagt tgctcagtca actgtatacc ttttgccttt aacttaagta
    17761 attagagcct cttatgcagt tgctcagtca actgtatacc ttttgccttt aacttaagtt
    17821 attagagcct cttatgcagt tgctcagtca actgtatacc ttttgccttt aacttaagta
    17881 attagagcct cttatacagt tgctcagtca actgtatacc tttcgccttt aacttaagtt
    17941 attagagcct cttatgcagt tgctcagtca actgtatacc ttttgccttt aa
//
LOCUS       DAHHYJ010000046        17979 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000046 DAHHYJ010000000
VERSION     DAHHYJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.046"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(141..407)
                     /locus_tag="NBK22_001150"
     CDS             complement(141..407)
                     /locus_tag="NBK22_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD2414345.1"
                     /translation="MSNYTVKIKNSAKLDLKKIKHPYLKKTFIEIVETLKNNPYKITQ
                     SFEKLEPKYLERYSRRINLQHRVVYTIDDRNKVVLILSAWSHYD"
     gene            complement(407..664)
                     /locus_tag="NBK22_001151"
     CDS             complement(407..664)
                     /locus_tag="NBK22_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD2414346.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETMYLVSTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            1023..1478
                     /locus_tag="NBK22_001152"
     CDS             1023..1478
                     /locus_tag="NBK22_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCD2414347.1"
                     /translation="MKKLCSLIVVALICIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KDDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            complement(1646..3223)
                     /locus_tag="NBK22_001153"
     CDS             complement(1646..3223)
                     /locus_tag="NBK22_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCD2414348.1"
                     /translation="MTFLTVMQFIVNIIVVGFLLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     VDGELQTIHDYKLKLIS"
     gene            3419..4168
                     /locus_tag="NBK22_001154"
     CDS             3419..4168
                     /locus_tag="NBK22_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414349.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAVFTQQAPWGFSFPISMNISYLLAVILSLAIFRRMHEDAFIPMIRSQIIAST
                     KYKWHILISFTLSIVLFLLLNYYNIFALNVDSFTVFDIYPLIRDYGSISLIVMLWLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            complement(4392..5585)
                     /locus_tag="NBK22_001155"
     CDS             complement(4392..5585)
                     /locus_tag="NBK22_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2414350.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTPR
                     PASSL"
     gene            complement(5597..6346)
                     /locus_tag="NBK22_001156"
     CDS             complement(5597..6346)
                     /locus_tag="NBK22_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414351.1"
                     /translation="MIKVTDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDQPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKEVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            complement(6339..7154)
                     /locus_tag="NBK22_001157"
     CDS             complement(6339..7154)
                     /locus_tag="NBK22_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414352.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGEIIFDGTSMLSLSEAQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEQGTRESVLHHPEHVYTKYLLSTKKKINDHFKHVMRGDVCD"
     gene            complement(7151..8020)
                     /locus_tag="NBK22_001158"
     CDS             complement(7151..8020)
                     /locus_tag="NBK22_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414353.1"
                     /translation="MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFVPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            complement(8017..8952)
                     /locus_tag="NBK22_001159"
     CDS             complement(8017..8952)
                     /locus_tag="NBK22_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414354.1"
                     /translation="MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIVFNTLADIINALLNPRLREGAR"
     gene            complement(8965..10563)
                     /gene="cntA"
                     /locus_tag="NBK22_001160"
     CDS             complement(8965..10563)
                     /gene="cntA"
                     /locus_tag="NBK22_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCD2414355.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLAKKWDVSEDGKTYTFHLRDDVKFH
                     DGTTFDADAVKKNIDAVQQNKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            complement(10706..12007)
                     /gene="cntM"
                     /locus_tag="NBK22_001161"
     CDS             complement(10706..12007)
                     /gene="cntM"
                     /locus_tag="NBK22_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCD2414356.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGKFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFSQDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LLQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQQLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            complement(12000..12818)
                     /gene="cntL"
                     /locus_tag="NBK22_001162"
     CDS             complement(12000..12818)
                     /gene="cntL"
                     /locus_tag="NBK22_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCD2414357.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNESVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            complement(12829..13650)
                     /gene="cntK"
                     /locus_tag="NBK22_001163"
     CDS             complement(12829..13650)
                     /gene="cntK"
                     /locus_tag="NBK22_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCD2414358.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKALEIIYETYVHYVIPVKQVTTE
                     IQHLVEAFVREQQWSHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            complement(14521..14886)
                     /locus_tag="NBK22_001164"
     CDS             complement(14521..14886)
                     /locus_tag="NBK22_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414359.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFDHE
                     VFDELYLAILKYLNTVSIENISHSELLNLIENTIPEDREINMFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            complement(15150..15968)
                     /locus_tag="NBK22_001165"
     CDS             complement(15150..15968)
                     /locus_tag="NBK22_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2414360.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDKAKAYIVDIASEQQVVDFVSDIKEQIGHVDVLFNNAGVDNAAGRIHEY
                     PTDVYDKIMNVDMRGTFLMTKMMLPLMMTKGGSIVNTSSFSGQVADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRANAIAPGTIEPPLVDKLTGTSEDKEGKAFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDESSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            16203..17741
                     /locus_tag="NBK22_001166"
     CDS             16203..17741
                     /locus_tag="NBK22_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCD2414361.1"
                     /translation="MTSKHQQKGSIVNRFLNSVERIGNKLPDPSVLFFLMCVGLAIMT
                     WVISLFKVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGIGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLCIPVGPGGP
                     IFVK"
     gene            complement(17853..>17979)
                     /locus_tag="NBK22_001167"
     CDS             complement(17853..>17979)
                     /locus_tag="NBK22_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCD2414362.1"
                     /translation="KKFEIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDRL"
BASE COUNT         5670 a         3471 c         2649 g         6189 t
ORIGIN      
        1 tcaatttgtt tagtcttgcg ctattataaa attaaaaagt gtagagataa caacgcatta
       61 acgtcgctat tttccctaca cttaaatact agtttcttta attaacttat cattaaagtt
      121 attcagatat tgaatattca ttaatcataa tgtgaccatg ccgatagtat taatactact
      181 ttatttcgat catctatggt atagacgacc ctgtgctgaa ggttaattct tcttgaatat
      241 cgctctaaat atttaggttc taatttttca aaggattgtg ttattttata aggattattt
      301 tttaaagtct caacaatttc tataaatgtc ttctttaaat aaggatgttt tattttcttt
      361 aaatctaact tcgccgaatt tttaatctta accgtgtaat tgctcattaa agattatccc
      421 aatcaatatc atctatattt gttgtaccac tattatcttt ttctctttct cttactttgt
      481 ccattgtacc agtagataca agatacattg tctcttgtat acttttccaa tcttctaaac
      541 ctataatcac agcattattt tcggcattat taccactaat ataaattggt tcgtgattat
      601 tattaacatt ttttagtaat tgataaaaat cttttctcgc ttctgtaggg ctagtgataa
      661 tcatataaat cctcctttgt cttctgttac gctaattatg gcgtacgtat ttaagtacgt
      721 caatatcacc gacacatccg tcgtaatctt agtctctata tgctatcgta cacaagcttt
      781 tgttcttttc ttccaacata ccgtctataa tgctcaaaaa gaaatcagca gtggaacaca
      841 ctacgttccc taccgcttta tcatttttca ggcttaaata tttttaaaat tttgtgagga
      901 atccgcttaa aaaactcata tcttcaaaaa aaatgacaaa atttaaattt ttgaataaca
      961 tcatttttta gtaaatgtta taatttcttt ataatacaaa tatcgaaaga aggcgactga
     1021 ctttgaaaaa attatgttca ttaattgtag tagcattaat ttgtatcatt gcattatcag
     1081 cttgtggtaa agaacaaacc aaaacatatg aaggtgatgt tagtggtaaa catgtactaa
     1141 cttctatcac atataaagat gataaggttt taaaacagtc tacaatcaac acaattaaat
     1201 atgacgatct aggtatggat aaagacgaag ccaaaaagtt attcgccaaa tctgaaagta
     1261 ttttcaaaga ccttaaaggc gtaaaataca aagtagacta taaagataaa aaagcaattg
     1321 aacacttaga catagactac acagaagttg acatgaaaaa attaaataaa cgtctcggtg
     1381 tttcaactaa agaaaataaa gatattagtt ttgaaaaact tgaaaagcaa ttaaagcaca
     1441 gaggtttaaa agaaaaagat aaaatggacg acaaatagtt tataacataa aaatgccctc
     1501 agataagact aaggttacaa accttaattc atattctgag ggctttaata tttgaaattc
     1561 ttgtgtgacc agcatccact actagtataa aattatttgc agtaacgcta aaatccgctg
     1621 ctttcatttt cccgaaataa ttaagttaac taatgagttt tagtttataa tcatgtatcg
     1681 tttgtaactc accatcgact tttcgatata caatatgatc agcagtaatt tctgtaggac
     1741 tggatacgcc aacagctgct gcaatattga ataagccttc atgcaaactt gttacatagt
     1801 ttgtgacacg atattgcttt tctccaacaa tcaatgcttt ttcttttttc gcatctgttg
     1861 ttgcaacacc aactggacat gtattcatat gacattgttg actcataata caaccgacac
     1921 taatcatcat gccacgtgca atattaacaa aatctgcacc taaacctagt gcaatcgcaa
     1981 ttttatctgg tgtcactaac ttaccagatg ccgccaattt cactttatct cgaataccat
     2041 atttttctaa catgccagac acaataggta gagctgtaaa tagcggtaag ccaacaccat
     2101 cttgtaattc ttggaatgtt gcaccagtac caccttcacc accatcaatc gtaataaagc
     2161 ttggatactt atctagttcc accatcgtac gtacaagtgt ttcaatttct gaaactttgc
     2221 ttactacaat tttgaatcct actggttttt gacctaattg ctgcaactga tcgacgaaac
     2281 gaatcaaatc ttcagcatta tgaataaatt cgtaacggtt aggtgaattg attgttttat
     2341 aaggttcaac atttcgtatt ttagcaattt cttcgtttac cttttcagct tccatatgac
     2401 caccacgagt cttagcacct tgtgccaact tcagctcaaa tgcgcgtacg ttagataact
     2461 gtgcgacctc tttaaataaa ccttcactaa aattaccttc tttatcacga acaccaaata
     2521 aaccgggacc aatttggaaa atgatatccc cattaccttt taaatgatat tctgataagc
     2581 caccttcacc tgtattcatc caagtgcccg ctttagctag acctttagat aaagctgtaa
     2641 tggcattttt tcctaaagcg ccataactca taccagattg tcctacgata cgttttaaaa
     2701 taaatggatg ttttaaatgt tcacctaatt ttattgcatg gtcatcactt aagtaatacg
     2761 gatcaatctt tgtcggtaca cgatattctt cacgactaaa taaacgctca ttcgcgattt
     2821 tataaatgaa tgttgataac aatgttgtat tatctactga aatctcatta cgttgcatcg
     2881 gaaacattgt gttctgtatg taaaagccgt cttgataatc tttagtagta ccgaagctgg
     2941 tcatacgaga gttatatttt ccagccaaaa cgatattttt ataatcatta cgtgaaaaag
     3001 gtttcccttc attatcccca gaaaataaat actgacgtaa ttccggtccc attttttctg
     3061 aaatatatct aatacgtgct agtaaaggat aattccttaa tacactatgt tgtgattgtc
     3121 ttttatcttt aattaaccaa ataagcccga taacaataac cgtaagcagg aagcctacaa
     3181 cgataatgtt aactataaat tgcatgactg taagaaacgt cattacaata cctcccccca
     3241 aaaatttcaa ttcaatattt atgatacacc gtacaaaata aaacacaatg gaagcgcttc
     3301 attttataaa acaattttat gatatgtttt tcattttaaa ttttaatgta taaaacatac
     3361 aatacaaagt aatatgtgct aaagtatcta tataatacaa ctatttaaga ggtatactat
     3421 gtcaaataca aataaacatt acatagaaga agaatacgct accgaacaat cgcgtttttt
     3481 caaacgtgat attggattta ttttccttta tatatttttg gttaacatct tgccaatcat
     3541 tatcgtcttt ttaacttttg ggttagtagc tgtattcact caacaagccc catggggttt
     3601 ctcttttcct atttcaatga acattagcta tttactcgca gttattcttt cattagctat
     3661 ttttcgtcgg atgcatgaag atgcgtttat tccaatgata cgttcgcaaa tcattgccag
     3721 caccaaatat aaatggcata ttcttatatc atttacatta agtatcgtac tctttttact
     3781 tttaaattat tacaatattt tcgcactaaa tgtagattcc tttactgtct ttgacattta
     3841 cccgctcatt agagattatg gttccatcag tttaatagta atgttgtggt taaccattat
     3901 attaaatact ttgatttgcc aacttatttt ccgtcatatt ttaattcaag aattaagcag
     3961 attaatacct ttaaagatta caatagtatt atccgttatc cttgaaacac tgtactatta
     4021 tccatacatt aatacatggt gggaattcat tccagcattg atactagcta gtagtgcgac
     4081 ttacttatat ttaaaatcaa accgtaattt tatggtgagc tatttttatc aattagcagt
     4141 tatactcgtt ttgcatattt tcatgtaaaa tatttcagag tattatccat tatccataca
     4201 catcaaaaag cacacttaaa agtcagtatc gcaactcact tctaagcgtg ctttatttag
     4261 atatactgtc gttctaaaaa cgagccatat tgaatgtttc atgtcctagc atgaagcaca
     4321 tgaatcgcca tgctcaatat accttttaaa atttaaatat tcattaactt aatatgcttt
     4381 aaactttttc attaaagact actcgctggg cgtggtgttg gttttttcaa taaactcata
     4441 atcattatta caatgactaa cataccacaa attagataca tccatttaaa tcctactaaa
     4501 tcggaaatag gtcccatcaa tgcgccacct aacgatatac ctaagtctgc acaggcaata
     4561 aataaaccta acaacatatt acgacctact tttggtaaga cgaaacttaa gtatgatgtt
     4621 aatgttggat acaccattgc ttgtgtcatt cctattaata ttgcactacc atagaaaata
     4681 attgcaccta cttgcggacc aaatgccact acaaatgacg cgatcactaa taatgatagt
     4741 acagatacca tatatttagg atgccacata ccatctgacg gaatgtattt ccttaagtaa
     4801 aatcttgccg caacaactgc gatggcctgt attgtcaaaa agattcccgc attcgcgaat
     4861 cctaaactca ctgtgtataa cggtacaaat gtactaactg caccaaatac aatcgatgca
     4921 acaatcatga taataccact gttcaacaac tctttatttt tgaaaaattg cgcaaaaaca
     4981 gttacagcgt taaacggcat tttttcaatc ttatctgacg tatcgggttc ctgttcagca
     5041 aaggtcacgc gatagccaaa gaatgttgtt gttaatgcga taaagataat gacaattgca
     5101 aatagtgaaa tattatttgc attccaaata cctacggcaa ctaatggtcc gattaagttt
     5161 ggaatcgttg aaaatagcga gtacaatgat acaccttcag aacgatgttc ctctggtaat
     5221 gcatcaataa tacctaactg taaagacata gaaaagaatg ccgtacacac accttgcata
     5281 acacgtgcta cgaaataacc ttctaatcca gtaaaaccat aaataattaa agctatggca
     5341 ttgataatca aaataattct taatacttta atgggaccga cacgagcaat aatttgtcct
     5401 gcccatggtc gaaataccat tgctgttaac atgtatgccc ccataacgat accgataacc
     5461 gtattcgttg cgcctaaatc atgccctcgt aacggtataa acacgtttaa gattgcattg
     5521 gcactaaaga acatcaatgt taatatatat aatctcaaaa agggccaagc cattgcacct
     5581 ttcattatct cactccttag aatgatagtt gtttttctat taattctctt gtataagcat
     5641 tgtcactttt atgcaatgcg cttgtcggaa tttgttcttc tatttttccg tttttaaaaa
     5701 taattaattg atcacataaa tacgtggcag cttgaatatc atgtgtgata aaaatataac
     5761 tcaactgacg cgtttcacgt aaatgaatca ataaatctaa tatttgtgtt tgaattgaca
     5821 tgtcgagtga actaatggct tcatcaaaca aaatatattt agggttaata catattgcac
     5881 gcgcaatcgc aacacgttgc gcctctccac ctgataacat attaggatat ttatccatgt
     5941 atgccttaga tagaccgact tcttccaaca atgtaattgc ttggacttcc ataacttctt
     6001 taggttgtcc atcacattga cacatcactt caaataagat ttctctaaca gtctgaaatg
     6061 ggtgtaatga tgacgtataa tcttgaaata cagcaccaat ttgatgacgt ctcactttct
     6121 tcttatgcat cggttgatca tttaaggtta cacaaccttt atccggtttc tcaataccta
     6181 atatcatacg actcaacgtc gatttaccgc taccactttc tccgataatc gcaatcgtcg
     6241 caccgattgg acactcaaat gacacacctt tcacgatagg tgttcgacga cgcttaaaaa
     6301 catgtgcgct ttgatatgac ttttcaacat ctgtaacttt aatcacatac atcacccctc
     6361 atcacatgtt taaaatgatc attaattttc ttcttagtag ataataaata cttcgtataa
     6421 acatgttctg gatgatgcaa aactgattca cgtgtccctt gttcaataag ctgaccattt
     6481 ttcatcacaa cgacacggtc tgctattttg ttaatgaccg ttaaatcatg cgaaatgaaa
     6541 atcattgcac agtcaaagtg tttctttata tctataaatg cttccagtac atcatattgt
     6601 gtaattgtat ctaaagccgt tgtcggctca tcagcaatga ttaactttgg tttcaaagct
     6661 aacgctaaag caatcatcaa tcgctgtaac attcctcctg ataacatgta agggtatgat
     6721 tttaatatac gtttaggatc tttcaaactt aaataatcca tatattcaat caatgtcttt
     6781 tcaatttctt gtgtagacat tgacgtatgt actttcatag tctcaaacat ttgtttaccg
     6841 acagtagttg atgggtcaaa ggcacgacta ccttgttgca tgaccatcgc aatatcttta
     6901 ccacggtatt ttttcaattg tgcttccgat aacgacaaca ttgatgtacc atcaaagata
     6961 atttcacctg tcaccccgag tcgttcggga ttcaaaccaa taatcgattt acaagtgatt
     7021 gatttaccac taccactttc tccaataacg cctaaagttt cacccttagt taatgtaaaa
     7081 ttcacatcac tcacgagtgg ttgatctgtc caggtatctg taatcgtcaa atgtttaact
     7141 gttaacaatg tcatgattgc accactcctt ttttcacaga acgaagttta tctttagaag
     7201 agatgcgggg atcaatagca atttgtaaag catcggataa gaagttaaat gccatcacta
     7261 taatcactat ggcaatacct ggcacaaaca tcatttcagg atgtgtaaac atcacttttc
     7321 tagcttcgtt aagcatcatg ccccactctg cagtaggcgc tttgacacct aatcctaaaa
     7381 atgaaaagcc agatatttgc aagatcattg aacacatcga gctactagag ataatagcaa
     7441 tatctgctaa tgttaacggc ataatatgtt tatgaataat tttcatatca ttcataccga
     7501 ttgttttagc aaatcttaca tggtcagaag cagtgtactg cataacactt gtacgtataa
     7561 cacgacagaa ccatgcccaa cgcgtcaaaa taaatgccat gataatattt tcggcaccca
     7621 ttccaaacaa tgcaattaat gctaacgtta caacataact tgggaacgcc aacataacat
     7681 cacacgcacg catgattaag gcgtcaacaa acccttggaa atatcctgat aagaatccta
     7741 aaatagatcc aataagtaca gaaacaaata gcgcaacaaa gacatataac aaacttggtc
     7801 taatcgcata aattaaccta gttaaaatat ctctacctaa atggtcagta cctagtagat
     7861 gttgaaaact catgccagca aatttgtttg ctgtatcgat atggttagga tcataaaatg
     7921 tcacaagtgg tgctgctaat cctaaaaaga catataatac aataatgcct aaagcaatca
     7981 ctgcaccttt atcttgtaat aatcgtttta aaattatcat cgtgcgccct cccttaatct
     8041 tggatttaat agcgcattaa tgatatctgc taatgtatta aatacaataa ataataccgc
     8101 tacaattaat acatatgctt gaataactgg aaaatcatgt tcaagtattg cttttaaact
     8161 taattgacct agtccaggcc atgcaaagat atactcgata acaactagtc cacccattat
     8221 cattggtata gacatacaaa agattgatac cgcaacttgt aaagcattac gcaatacatg
     8281 caacattaat gtgatagatt tcacaccgct tgctcttaaa taaagtacat aatcttcatt
     8341 taattgttcc accatcgagc gtctaacatt tctaaagtaa ataccagcat aggcaatcgt
     8401 aataacgatc actggcaata tgtaactttc tggacctgtt aatccagaag tcggcaatat
     8461 gtttaacttc actgaaacgt aaataataag tattgaagct atccaatatg atggtaatgc
     8521 agttagaaag aaagccactg aacgtatcgc acgatcagtg aactttcctc tttttaatgc
     8581 actaactaca cctaaaataa ttgatgtaat catcaccata acacttgaaa ttattgttaa
     8641 tttcaatgta ttcataaatg ctggaccaat acgttcagca actgggtcac ctgtaatgta
     8701 gcttgtacca aaattaaatt gcatcgcttc aagtaaccaa tttttatatt gaattaataa
     8761 tggatcattg aaaccgtact tctcattcgt ttctgcaatc aactctggtg ttacatttgg
     8821 cgttccttgt gcatgtaaaa ttgtcacagc tggattttca tttgtaatat acgtcaatag
     8881 aaatgtcata aaacttacta caatcactaa tggaaacatg agcgcaatac gttttaagat
     8941 aaatttgaac atctaattgc tcctttattt atactgcatt tcattgaatg gtaattcata
     9001 ctgtgattgt gtgaatgata ctttttctaa atctttcggc gcaacaactg tcatactacc
     9061 gtgtgaaata gggataaaga taccttcatc atcaatttgt ttcaaaatgt ttttataagc
     9121 gtctgaacgc tctttaccgt tttggatttt aaatgcgtca tcaatgctgt tgtatatttt
     9181 atctttgttc tcaatgcctg atgttgcact ttcataacca tttttcgctt taaatgctgc
     9241 aatagtactt tgtggatcgt acaataatcc ccaagtttgg ttgaacatta agtcataatc
     9301 accagaagta cgacgttcag caattttatc tgatgtttcg ccattgatgt ttaacttaat
     9361 acccattttc ttaaattctg cttgtaagta ttctgcttgt tctttttgac ttgaagaacc
     9421 tttgtcatag tacattgcca tttcaaggtt tttaccatct ttttgacgaa catcactgtc
     9481 tttacctttc ttccaaccag cttcatctaa taatgattct gcttttttaa ggtcatactt
     9541 acgtgttggc atatcgaaat taatgtctgt tacatttttc gcaaataatt gagttgctgg
     9601 tttttcttga ccatctaaaa tttctttggc aattttatct ctgtttacca tatgaccaat
     9661 cgcttgtctg actgttttgt cactgacagc gttatctttt ttaccagaat tgacaactaa
     9721 cattttcgta ttcataggtt gactacgctt aacttgatag tcacctgtat ctttcaattg
     9781 ttttaaagag tctttgtcta agctatccgt acctctatca tctgtgaagg caaagttcgt
     9841 ttcacctttt ttcattgata ggaatgctgt ttcaccagca ggcattactt ttgcttgtac
     9901 tttgttaagt ttagactttt cgccccagta ttgatcattt ttgttaaagt ctgcagactc
     9961 atcttttttg tgttcaccta atttaaatgg accagtacca tcgaactttt taacgccatc
    10021 ttttgttgta ccgtttttaa agtcttttgg agacacaaat acatatggac gaggcatcgc
    10081 taattcagcc aatgcaggtt gatatgcttc tttcaaattc aattcaaccg tgtacttatc
    10141 tttaacttta acattgtcaa ttaatgttga aatctttaac caagaatgca attttttatt
    10201 ttgttgaacc gcatcaatat ttttcttaac tgcgtcagca tcaaatgttg ttccatcatg
    10261 gaatttcaca tcatctctta aatggaatgt ataagtcttc ccatcttcag acacatccca
    10321 ttttttagct agtaaaggct taataccatc tttcgtgtta cgtacaagcg gctcgtatat
    10381 catactttca gcagacattg atccaccgta aacatgcgga ttcatatcac cgatatcttt
    10441 aaccgtcgta tacgttaatt gcttgttttc ttttttctcc tctaaacctt tattaccgcc
    10501 acaaccagtt aaaattagcc ctgatgcaag taacattgca ctcattttag ttagttttct
    10561 catttgcttt tcctctttct aaattgataa gttgcattac ttaatcaaat cgtaatgatt
    10621 atttttctca attatttgct tatttttatg taaatgtcaa acataattca ataatttttt
    10681 tctattataa aacttcaaac ccttattatg aaagcgttct attgatttcc aaaaattttg
    10741 tgacgagtgc cttatctcct tcaaatagat ttgtattaaa ttgagaagat agttgttgat
    10801 cttggtgcat atccttgtac gcctggcaac ttgcttcata gcgagttagg aactgatcaa
    10861 tcattggcgt tttgatacct aacattttcc caatatgctg aatcatcgcc gttctgtaat
    10921 aatcttcact tggcattctt ggaatttgaa caacatcctg ttcattttta tagacttgct
    10981 taaatggtac agctgaaaaa tcaaagtaat gtccgttttc gtctggctgt gaaaatggat
    11041 caatgaggat tgcggtatat cttacataaa gcagatattc ttgtaagata tctggcaaga
    11101 tttcgaaatg ctcaatatca ccttcatcca aagtttcagg acgtactgga taattttcct
    11161 tcaccataaa ttgaagcagg ttgactgacg gcactctaaa tgcttgtaaa atagccatca
    11221 tttccttcca cattaaacgc atttcacgga ttagtgtcat cgttatcggt ccttcaggaa
    11281 ataacttata cacataaacc ggtacatccg tcccttcgaa aatggctttc aatgaaaagt
    11341 cattcataaa tagtggtggg tgcacataaa gcgaactgtt tcgcgtttca gcgtgcagtg
    11401 gcgattcaac gacttctaat tgaatcttca attgctcagc taaagcagag attcgttgac
    11461 acattgttga gtttgaatgt gtcgatccca tgtacaattt cttttttaca cctgttgtca
    11521 acacatgatt aggcgcttct ttatcaacaa tacgtgtatc gccaagataa gttgaaaatg
    11581 aaatcacttc gatatcctga ctaaatttag acataaattg ttcgacaatc atttgcgaac
    11641 caaatgtcgg tgatattaaa atgacatgtt tgacactttg caaagtttct aacgacaatt
    11701 gctgtagtgt gtcataataa gcatctgctg tgcatgccat gacaaccgtt tcgtattcac
    11761 ccttaacgtt tttaacatct ttatacaaac gattaatttc aaacttacct tccagatgtt
    11821 gatgcgcctc gttttgtatt ttgacttcaa attgtttctc ttttttatac gcttgatata
    11881 agcgttttga tttttctgat gttgaggcac gtccaaccat atcaatctca taatctgatt
    11941 ttaaatagca aatattcgct aattggattg cgaccggacc agtgcctatc attaataatt
    12001 tagacatccg taatacctac ctttatagct gcttttttat aaagtgctat atcaaaaatt
    12061 tgctgtggtc tcatatgttt attcacacat tgccacttat cttccgctgt ttcttgtgac
    12121 ggataattaa atattgcttt gatgccatca ccaaagcgca ttgcaactac gacactttca
    12181 tttgttaaat catataattc ttctaaaatg ctgtacttta aaggaattgt cgagctgaat
    12241 atgatatgcg tcacatcttt gatatcttta agttcagata ccttttgatc cgtaattgtt
    12301 atatcttcat ttggtgctaa gacgttaacg attctacgtc ctaggtcaac ggcttgtgga
    12361 tcaatatcaa taccgataac tgaagcacct gtttcttttg ctacttgaat taacgtcatt
    12421 ggatatgcac ctgaaccaac taacaataat ttgtcattgc tagctatact acattgtcca
    12481 aactcttcat caatacaatg ttctatattg tcaaagtaac ctgtggtaga cgcctgtccg
    12541 tctaatagac gtctagctct aatgacttcc acttgtttaa cacactgcgc tgtaatatat
    12601 tgcagtgatt ttattaattg tgctttttct tcaacgtcac gccaagcatt aaattgttgt
    12661 tcataaatag gatttaaaat aaattcacta tagtcatcca tcaatgtttc taatgcttcg
    12721 atatattgat cgtcttgtaa tacacgctcg taatgcgctt caaacttttc caaatattgt
    12781 tgtaatatca atttgatttc attattaaag ttattcattg taggctcctt attctatata
    12841 tgcttttcct gtagctacag ttgtaacctg tcctttaatt gaagtttgat atcccaattg
    12901 atgacatcgc tttgatgtca ctaaaatact gccccctggc tgatgtactg taaaatcttt
    12961 gcatgcgtca ttacgttgat aattattaaa aaccccaatt gatgctgtac cagaaccaca
    13021 gctattttcc caaattaaac tttgaatttc tggtatatag attaatggct gtaaaaattg
    13081 acgttgttca tcaaaaagca tcatacctac tgttttatat ttgtgactcc attgttgctc
    13141 acgcacaaat gcttcaacca aatgttgaat ttcagttgtt acttgtttaa ctggaatcac
    13201 ataatgtaca tatgtttcat aaataatttc taatgctttc catgaatgat tacccatatt
    13261 aattgttgtt ggcacaacac gatgggcttg tggcatttga acttcatagt attggcaatc
    13321 atgaattgcg cattgcacta aatccgaaca gccagacacc ttcaccttaa actgttggtc
    13381 tttaagcaaa tgactttctt gcaaatgatg tatatatgac atcgtcgcat taccgcaaaa
    13441 ttcattaccg ctcataacta gatggaaatc attgccatca tcattttgtg ttgattctat
    13501 aaagcctacc tgttcacaac atacatgtgt tgcggccatc aactgattgg cgatagatgc
    13561 atattcacta gcatcatgtt ttgaatgaac aagtatcgtc atattccccg aaggattata
    13621 cttagaaaat tctataacct gccgattcat acgacactcc tttagatgta tttttagagc
    13681 ctctgtaatt ttaattacta taggctctta tgcagttgtt gcgaagcaag caactgtatt
    13741 cctcttaacc tgtaattttt tagaagatga acctaatagc tctatctagt aggtaagtca
    13801 tgctaattcg caaaagcgaa aaccatggct cttatgcagt tggtgcgaag caagcaactg
    13861 tattcctctt aacctgtaat ttttaataga agatgaacct aatagtttcc catctattag
    13921 gtaagtcatg ctaacccgca aagtggaaag catgacttag taatcggttg ttgtatcaac
    13981 atgcaaccgt aaaccttatt taaagttgct tcttgtggct ctacaagcga atgcagttgt
    14041 ccgttaacta cattcactta gatgctttta ctgttttcac ctcaactaaa acttcaaaaa
    14101 agttgcagaa aacaaatcgt aactattacg atttgattat atactatatt agttgagaat
    14161 cattgtcaat aaaggttgta aatgaatgca catttatatt ttgataatct ttccataaac
    14221 atcgtattct cagcacattc aagtcttttg aaaattttta tcaataattg agtagaagtt
    14281 tcgttctcgt cagttcaaaa ttttacgtaa gttgtctctg cgtattgctt tagtgagaat
    14341 aacccacact tgtggaaacg tccttttgtt aatctacaat ggataccctt gtctttcgct
    14401 aacatttcct attaccaatc ctgtaacgga ttttcatcgt caagttgttg cccatacaat
    14461 acgcaccaaa aaaagctttg tacaatttcg tacaaagctt taaaaattta aataaaaata
    14521 tcattgtttt aatgaactag ccatatcaga ttttatttcg tttgctaata cttgtaattc
    14581 agggaaataa ttattagcaa agccaattat tattttattt tttacaaaca tgttaatctc
    14641 tcgatcttct ggaatcgtat tttctattaa attcaaaagc tcactatgac ttatgttctc
    14701 tatacttact gtatttaaat attttaaaat cgcaagatac aattcatcaa atacttcatg
    14761 gtcaaaatca aagtccaata atagtaaact aacggaatta gtttctaact gattatctcc
    14821 aattttatct gcaatctctc ttatagcttg atatagcaat ctaaaacttt ctttttcttt
    14881 taacataaaa ttctccttaa tttattagaa tagacaaaat acagcttatt ttaaagatac
    14941 tccaaaagtt tgaactcgag attctgataa attagatgca cgacctacaa tacgttaaaa
    15001 ctagccttaa gcaatacact tcaaatgaac aatatgtgat tttcaaagat taattaatct
    15061 ccgctttata tgtctttcat tgcgacaata tgcatacatt gcacacttca tgccaaatta
    15121 ttttccactg gatttcgatt ttaaaacaat tattccaatg tccgcttcca cgaatcatca
    15181 ctcaacatct cgccaggcca tgtgtaagcc ataacaccac cgtcaatgcg aattgtttca
    15241 ccagtaataa atgaactctc atcagatgct aaaaagacaa cgagcttgcc tacttcttct
    15301 ggtttaccta aacgtcccag cggtgtcatc catttctgat tttctctaaa tgctttgcct
    15361 tccttatctt cgctagtacc tgtcagttta tctactaacg ggggttcaat cgtacctggt
    15421 gcaatcgcat tagctcgaat gccatctcta ccatattcga ttgcaatgga tttcgtaaaa
    15481 ttaatcactg cccctttcgc agcattatat cctgagcgat acaaatctgc tacctgtcct
    15541 gaaaatgaag atgtgttaac aatagagcca cctttagtca tcattaaagg taacatcatt
    15601 ttcgtcatta aaaatgtccc acgcatatct acattcataa tcttgtcata tacatctgtt
    15661 ggatactcat gaattctacc agccgcatta tccacaccgg cattattaaa taacacatca
    15721 acatgcccaa tctgttcctt tatgtcagat acaaagtcta ccacttgttg ttcgcttgca
    15781 atatccacaa tataagcctt tgctttgtca ccattacttt taattttatc gacagtctcc
    15841 gataccgctt cagctatgtc taccgccaat acatacgcac cttcttgagc taaagcgatt
    15901 gcagaagctt gtccgatacc tgtacttgct cctgttacga ctgcaacttt attttccaaa
    15961 cgtttcattc agtcactcct atacaacata tcttaaactt acagtcctta ttttccactg
    16021 ttcaatgctt cataaaccta atagatacgt ctccaggcag tcttaactac tctatgctat
    16081 acaatttcta ttgaataact caaaatattt aacaatatta gatttttcat tttctgaaaa
    16141 ttgttacttt tgagagtatt ttttgttaca ctttaaatat ttaacaaaag ggggaatcat
    16201 tgatgacatc aaaacatcaa caaaaggggt ctatcgtcaa tcgtttcttg aatagtgttg
    16261 aaagaattgg aaataaattg ccagatccta gcgtcttatt ctttttaatg tgtgtgggct
    16321 tagccattat gacgtgggtt atctcattat ttaaagtatc tgttaagcat ccaggtacgc
    16381 atcaaaccat ttatattaaa aatataatta gccatgatgg atttacgatg ataatgaatg
    16441 atacgattaa aaatttctca gagttcccag cattaggctt agtactagca gtgatgattg
    16501 gtattggtgt tgcagagaaa acaggatact tcgacaagtt aatgatttct gttgtgaatc
    16561 gcgcaccacg atttttaatt ttaccgacta ttatattaat tggtatttta ggtagtacag
    16621 ccggcgatgc tgcgacaatt atcttgccgc cgcttgcagc aatgcttttt attaaaattg
    16681 gctatcaccc tatcgctgga ctaacgatgg catatgcttc tgctgttgga ggatttgcag
    16741 caaatatagt tgttggtatg caagatgctt tggtctattc atttacagaa ccggcaacac
    16801 gtatcgtttc agattctatt aaaacaaacg ttgctatgaa ctggtacttt atcgcagcga
    16861 gtgtcgttgt attacttcct acgatattgc tagttacaac taagctcatt attcctagat
    16921 taggaaaata tgatgacagt ttaatgcatg atgaccatga agaaacatct tcacatatta
    16981 ctgacaaaga agcacatgct ttaaaatggg caaatatcag tttcatagtc acaattattt
    17041 tattaattat tacagccatt cctgaacata gctttttaag aaatgctaaa acaggcagct
    17101 tactagacga tgcgccatta attaacggta tcggattaat tattcttgtc gtatttttag
    17161 tacctggatt agtttatggg attttaagca aagaaattaa aaatacaaaa gacttaggta
    17221 aaatgttcgg cgatgctgta ggatcaatgg gaacatttat cgttattgtg ttttttgcag
    17281 cacagctact tgcgtattta aagtggagta atttaggaat tatcgctgct gttaaaggtg
    17341 ccaaattatt agaacatcaa aacggtattg tattgatact aggtattatt gtactgagtg
    17401 caatggtcaa catgttaatc ggaagcgcat cggctaaatg gggtatttta ggaccgatat
    17461 tcgtaccaat gttaatcctt atcggctttc atcccgcatt cacacaagtc atttaccgtg
    17521 taggagattc aattaccaat ccaattacac cgatgatgcc gtacttacct ttattattaa
    17581 cttatgcaca aaaatacgat aaacgcatga aactcggagc cttactttct agtttaatgc
    17641 cgtattcaat cgcgctaagt attgtgtgga cagtatttgt cattatttgg ttcctattat
    17701 gtatccctgt tggaccaggt ggaccgatat tcgttaaata acagtattca aagggataaa
    17761 ttaaaatgcc actatacaag atttgacctg tatagtggca tgatttgtta tgatatagcg
    17821 ttcgagatga aactctgcat ttatatgaac aattatagtc tatcactaat aatatttagt
    17881 ctgttccaac cgttaatttg gtttacaaca aagacaagat caataatttg ttcttcatca
    17941 taaaatgatt ttagccggtc aataatttca aacttctta
//
LOCUS       DAHHYJ010000047        17583 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000047 DAHHYJ010000000
VERSION     DAHHYJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17583
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.047"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(285..2093)
                     /gene="pepF"
                     /locus_tag="NBK22_001168"
     CDS             complement(285..2093)
                     /gene="pepF"
                     /locus_tag="NBK22_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD2414363.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(2141..3127)
                     /locus_tag="NBK22_001169"
     CDS             complement(2141..3127)
                     /locus_tag="NBK22_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCD2414364.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPITRKL
                     HTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKISELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAIYQLQLTDQEVVHNYGYIFPEQIYIENHPIEWQLQVDLWLK
                     NGKSKLVSDNLNYFKLKKFIVGLESKTAIIEKLINNYLNICSDRGNDVQILF"
     gene            complement(3248..3967)
                     /gene="mecA"
                     /locus_tag="NBK22_001170"
     CDS             complement(3248..3967)
                     /gene="mecA"
                     /locus_tag="NBK22_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCD2414365.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKEAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
     gene            complement(4338..4733)
                     /gene="spxA"
                     /locus_tag="NBK22_001171"
     CDS             complement(4338..4733)
                     /gene="spxA"
                     /locus_tag="NBK22_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCD2414366.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNEDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            5028..6017
                     /gene="trpS"
                     /locus_tag="NBK22_001172"
     CDS             5028..6017
                     /gene="trpS"
                     /locus_tag="NBK22_001172"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCD2414367.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPTHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
     gene            complement(6087..7709)
                     /locus_tag="NBK22_001173"
     CDS             complement(6087..7709)
                     /locus_tag="NBK22_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2414368.1"
                     /translation="MNISKVKYLLLSFIFVLILSGCSKEGIYSDKGQVFRKVIPQDMS
                     TLDTALITDAVSGDISNQVFEGLFTLDENDKAKLALAKELPKKSNGGKTLTFKLRNDA
                     KWSNGDPVTANDFVYAWRKLVDPKTGSEYAYIMSDIENADSINAGKLPVEKLGIKALS
                     KYELEIKLKRPIPYINELLALSNFYPQNEKVAKKFGEKYGLKPQNAVYNGPFKVDTWR
                     AEDKILLSKNNFYWDKKNVKLDKVNYKILKDKQAGASLFETGSVDDTLILADQVKKYE
                     NSKALQKRLTSGNFFIKFNQKKVKEFKNRNLRIAIALAIDKKGYVEAVKNDGSTFSDT
                     FTAKGVAKLSNGDDYASSVNSPLKYDKKKALEYWEKAKKELNIKKFTFSMNTEDTPDS
                     KISAEYIKAQIEKNLPGVKMKIRQLPFKQRLSEEFKMTYESSLTGWAADYPDPTTYLS
                     TMTKDNPQNNTDWGNKKYDDYLGEINGKLLGDVSERDSKMKEDEELLLKEAPLAPIYQ
                     KGEAHLTNPKVKGLQYHVVGPDTTLKYVYIEK"
     gene            complement(7756..8663)
                     /locus_tag="NBK22_001174"
                     /pseudo
     CDS             complement(7756..8663)
                     /locus_tag="NBK22_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795070.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8653..9735)
                     /locus_tag="NBK22_001175"
     CDS             complement(8653..9735)
                     /locus_tag="NBK22_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414369.1"
                     /translation="MVRKVLEVKNLEVSFEVEDGIVNAVRGIDYCINEGEVLAIVGES
                     GSGKSVATKAITKLFQGDNGRITNGSVVFFGEDLVNKNEKEFTKIRGKDIAMIFQDPM
                     TSLNPTMQVGKQIMEPLMIHKSMNKKDAKVKAIEILDLVGIKNAELRFKDYPHQFSGG
                     QRQRIVIGMALACEPKLLIADEPTTALDVTMQSQIIELMKKLQKKTNTAIIFITHDLG
                     VVANIADTVSVMYCGEIVETGKVEELFYNPKHPYTWGLLASMPTLESNSKSKLIAIPG
                     APPNLLKPPTGDAFARRSAYALNIDFKYEAPWFQLSPTHFVKSWLLDSRAPKVIPPEV
                     IKEKIKNLPYNFERPLKVEGVSFDEK"
     gene            complement(9748..10767)
                     /locus_tag="NBK22_001176"
     CDS             complement(9748..10767)
                     /locus_tag="NBK22_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414370.1"
                     /translation="MLIDENNKQLTSTDFELKKDNMNQEFVNVKNKSFWQDAWTQLRK
                     NRLAVIGMIGLIILILFALFGPAISQHNSTEQQVTDRNLPPKIPLLGKVDYLPFNDKD
                     VNGKDTYKEVGSKDYYIFGTDQLGRDLWTRTWEGARISLFIGVVAAILDVFIGVLFGA
                     ISGFYGGKIDTVMQRIIEVIASIPNLIIVILFVLIFEPSIWTIILAMSVTGWISMSRV
                     VRGEFLKIKNLEFVQASKTLGASNKRLIFKHILPNTLGAIIVTSMFTVPSAIFFEAFL
                     SFIGIGVPAPQTSLGSLVNDGKSMLLIYPHELFIPATILSLLILFFYLFSDGLRDAFD
                     PKMRK"
     gene            complement(10767..11693)
                     /locus_tag="NBK22_001177"
     CDS             complement(10767..11693)
                     /locus_tag="NBK22_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414371.1"
                     /translation="MIKYILKRIFYMAISLVIITTITFFLMKLMPGSPFNDAKLSPDQ
                     KYILNEKYGLNDPIPIQYLNYMKNVFTGDFGYSFQYHNQPVWDIIKPRLIPSLEMGIT
                     AMIIGMLIGLILGVASAAKQNTWIDYLATIISVLAVSVPSFVLAVILQFVFAVKLRWF
                     PVAGWEGISTIILPSFALSAIVIATVTRYIRAELLEVLKSDYILLARAKGVSSKKILY
                     THALRNALIPLVTVLVPMMASILTGTLTIENIFGVPGLGDQFVRSIATNDFPVIMATT
                     ILFSSFFIVSIFLVDMLYIIIDPRIRIQGGRN"
     gene            complement(11870..13525)
                     /locus_tag="NBK22_001178"
     CDS             complement(11870..13525)
                     /locus_tag="NBK22_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2414372.1"
                     /translation="MTRKFRTLILILIATFALSGCANDGGIYSDKGQVFRKILPQDLT
                     SLDTSLITEEMSFEVTAQTFEGLYTLGKDDKPVLGVAKAFPEKSKDGKTLKVKLRGDA
                     KWSNGDKVTAQDFVYAWRKTVNPKTGSEFAYIMGDIKNASDINAGKKPVEQLGIKALD
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGENYGTAADRAVYNGPFKVDDWK
                     QEDKILLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDALITADQVNKYK
                     DNKGLNFVLSTGTFFVKMNEKEYPEFKNKELRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWEKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSGNYQASLSGWSADYPDPIAYLE
                     TMTTGNAQNNTDWSNKEYDQLLKDARNELALQPTERYESLKKAEEIFLNDAPVAPIYQ
                     KGTAHLTNPQVKGLIYHKFGPNSSLKNVYIDKSIDKETGKKKN"
     gene            complement(13544..14485)
                     /locus_tag="NBK22_001179"
     CDS             complement(13544..14485)
                     /locus_tag="NBK22_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414373.1"
                     /translation="MKTDEILLSIKNLKQYFNAGKKNEVRAIDNISFDIYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGTDIQKIKKRKDLLKFNKKIQMIFQDPYAS
                     LNPRLKVMDIVAEGIDIHHLASDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQRERGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPSEEIYQNPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EENNHLRRLHEIRPNHFVFSTEEEAAQLRENKLVTQN"
     gene            complement(14475..15557)
                     /locus_tag="NBK22_001180"
     CDS             complement(14475..15557)
                     /locus_tag="NBK22_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414374.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLGEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQRMKPMPNNYEKPLMVERVSFNEN"
     gene            complement(15573..16643)
                     /locus_tag="NBK22_001181"
     CDS             complement(15573..16643)
                     /locus_tag="NBK22_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414375.1"
                     /translation="MAENKNNLSINDDHSNAAMTHTSDAIASSDFIIRELDLNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVEHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            complement(16643..17569)
                     /locus_tag="NBK22_001182"
     CDS             complement(16643..17569)
                     /locus_tag="NBK22_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414376.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
BASE COUNT         5560 a         3188 c         2351 g         6484 t
ORIGIN      
        1 acatatatga atcgttaatg tataaaaagt gcatatttaa tacattttta tacaactcca
       61 cttccctctt attaaaaaag aacataaaaa aacgtcgtgt ctgaatacac gacgctaaac
      121 aaaggggatg ggagaaattt ttcacttcaa acaaaggggt atgtttgtta tgtgattaat
      181 ttcatgttaa taatataaca tgctatctct accatttcaa ccttttaata gtacttaatt
      241 taaattgtca caatctgttc ataatgtaag cgaatacaat agtgctaagc tttcattaat
      301 ttttcaaaag cgttcaattt ttgttcaaaa acttcacaag cttgttcaat tggttcaggt
      361 gttgtcatat ctacaccagc attttttaat atctcaattg gataatttga gctacctttt
      421 ttcaagaatt cattaatata tctatctact gctggcttac cttctgttaa aatttgatga
      481 cttaagcttt gagctgcact gtaaccagtt gcgtattgat atacataata attcatatag
      541 aagtgtggaa tacgtgacca ttccttacta atatcttcat cagtttctac agaatcaccg
      601 aagtataatt tatttaattt ggcatattct tcattcattc tagttggtgt taaaggttca
      661 cctgcttctt caattgcatg aattttatgc tcaaattctg cgaacattgt ttgtcggaat
      721 aatgtagctc tgaaacgttc taattcttgg tttaataata ataagcgttt ttcatcatca
      781 agatgtttat ccatataatc acttaaaagt gcttcgttac aagttgatgc aacttcagcg
      841 acaaaaatag tgtagtcact agaatttgac ggttggaatt ttctactgaa gtaactatgt
      901 gctgaatgcc caaattcatg aactaatgtg tataagtctg aaatagtatt agaccagtta
      961 agtagaataa atggattagt taaatgtgca cctgatgaat agccacctga acgtttacct
     1021 ttattctcat agacatcgac ccaacgattg tttaagcctt ctttaactac atttaaatat
     1081 tcttcaccca ttggttctaa agcttttaac atccactctt tagcttcttc ataaggcatt
     1141 tcaaacttaa tatctttaat taatggtgta tataaatcat acattttcaa gtcatctaaa
     1201 cctagcaatt ctttgcgcaa ttcagtatat ctatgtagca atggtaaata tttatgtaca
     1261 gtttttacta gattgtcata tacattttct ggaatatgat tattacttaa tgctttttct
     1321 cttgcagttt tgtaattgtg tgtacgagca ttaaatacat ttttcttcac ttcacctgct
     1381 agcgtagcgc caagcgtatt attatgagca ccatatgctt tatatacatt tctaaaagca
     1441 ctttctctta gtttgcgatc atctgattct aaatacttaa taaatgtacc ttgtgttaac
     1501 gggtgtgcat ttccatcttt atcaatcgca tcttcaaata ctaaatcagc gttgctaaac
     1561 ataccgtata catttgatgg cgttgataac gcgtcctgcg cttctgttaa taacttttca
     1621 gtttcagcat ctaaaatatg aggacgtttt tcattaatca acttcaaatc aaatgcgaat
     1681 ttttgtaatt tatcatatga atttacaaat gattgaattt tatcttcatc aatttgtaaa
     1741 atctctggca ctaagaaact ccatgccgag ctaaatttaa taattaattg atgtgctctt
     1801 gactccatac cagtatactt gtcgttcgtt gtatcttggt cttgttttag gtgcgcgtat
     1861 acatatactt tttctaattt agtacctaat gtatcttcta attctaatgc attgtataat
     1921 gtctcagcac tatcaccaat gtgtccttta aattgttctt ctttgcctaa ctcattttca
     1981 acttctttaa atgcagcctc aaaagcttca tcatctttga aaattgttgt taagtcccat
     2041 gtatattcag gatatttacg ttcctgttct tctctagata attgttgact catatgtagt
     2101 aaacctccta ataaaatata attgatataa ttttctcatt ttagaacaaa atttgcacgt
     2161 cattacctct atctgaacaa atatttaaat aattgttaat aagtttttct ataattgctg
     2221 ttttactttc tagaccaaca ataaactttt tcagtttaaa ataattaaga ttgtcactta
     2281 ctaatttgct ttttccattc tttaaccata aatcaacttg taattgccac tcaattggat
     2341 gattttcaat ataaatttgc tctggaaaaa tataaccata attgtgcact acttcttgat
     2401 cagttaactg taattgatat attgcactta aagtgggttc taaaacagaa ttttgccagc
     2461 gacaatattt gatatattga ttaattgcga actttgataa tttataaaca gcatgataat
     2521 cacaaggcgc ctcattaaac aactcactaa ttttggcatt ccttttttca gcgacatatt
     2581 tgtgcccgcc taaatattgt atttgttgaa acatcattat ttgttttttc tctaaattga
     2641 acgtatgaag ttttcgagtg attggattaa taaacatact ttgaaaatga ttgaacttca
     2701 ctttattatg acaataatca acatcattta ataaccaact tactttatat cccattttct
     2761 ttaaaccttc cgttcgttga agaatctgtt tataaggaat tggcgaatac tgtagctcaa
     2821 taacatattt attattaatc aaaatatccg gaatttgttt tatttctttt aaaaatggct
     2881 ctatctctac tttacatcct tgttgctgta tcatctgtgc taaaatcaat ttacttttat
     2941 aatgtttaat cgtttcatta ttaaaacatt tttgttccgc taaatgttta tgtgcaaaat
     3001 gactaattac tttgagccca cgctttaaaa taacttgctt gccacacacc ggacaaaaat
     3061 attgtgtctt tctcaatgca gtagttgcta acacacgttc cttttcttca tttaaagcta
     3121 ctaacatatc ttcacctcaa aatattacac gtaagtttaa ctactattac tttttcagct
     3181 tttgaaatca ataaaaaaag agactccaaa gagtctcctt tcatagtgcg ttgcttaatt
     3241 aatatcgtta ttcagttgtc tctggaaaat aacgtcgaac ttgagctgtt acgttatgac
     3301 tcataattat tttagcatag tcatttaaat aaacttctgt tctgtctgtt ggataagcaa
     3361 attcaagcaa ttgactgtaa ctatcattaa tgacttcttg atcaacatga ctatcaaaat
     3421 gtacagcata ataataagta ccatcaacca tatataacaa atcttcaaac tctgtagtta
     3481 ttggattgct atgatatgca taattaataa catcttctaa atcgttaaat ttcacaataa
     3541 ttgttcttgt atttttacgt gctgaagact tttgacgttt agaaccttga gcttcttttt
     3601 cttttgttcg ttgctcgaat aattcttcta attgatcttc accttctaat gtttgagcta
     3661 acaattcttg aacttgttca tcaaattgat cagttgcatc atcatcagac atattcatca
     3721 tatcttcatt tttagattta gaaattgtga cttcgacacc tttttcaaag gcatgtactt
     3781 gaatccataa tggaccttct acaacaaaat cttcttcttc gttaatttca tccatcattg
     3841 accaaaagaa ttcttcgcca cgtttgcgat ttgtccataa atcttcacga ctaaatccac
     3901 gggcctcgat atcgctatat gttataaaca attttacagt tgtatcatct actcgttcta
     3961 ttctcatatc atctcactcc ttacagtcga tgaatagtat ccacattgta ttaaagcatc
     4021 aaacaaaata caatttattt gtttgatttc tttctctaaa atattttggt aacttataat
     4081 aacatattcg ttgtagcaaa actatttaaa tgatttatac agttaaagca ttaaagcact
     4141 ttcttaattt aattttacct tatcatagca aatgtttcat ccaaaacact ctaatataga
     4201 gctatattaa caattgcaca tttgatttaa aagatactaa gcaattattg taaaattaaa
     4261 accaaaaaag caacactata gaaatagtgt tgcttgacat atcttttagt aaagtatgac
     4321 acatagttaa atggttatta gtcaaccata cgttgtgctt cttgtaattg gaacgtacga
     4381 acttttctag gtaagaaacg tcgaatctcg tcctcattat aaccaacttg tagtcgttta
     4441 ttatctaaaa taattggacg acgtaataag ccaggattat cttgaatgat tgaatataag
     4501 tcttgtaatg gtagtgaatc aatatcaaca tttaattttt ggtatgtttt agaacgtgta
     4561 gaaatgattt catcagtacc gtcttcagtc atttttaata tttgcttaat ttcatcaatt
     4621 gttaaatgtt cagaaaaaat attacgctcc gtatacggaa tgtcatgttc ttgtaaccat
     4681 gctttcgctt tacggcaaga tgtgcaactt ggtgaagtaa ataatgttac catacatctc
     4741 actctcctat ttgaatgaat aaaattcatt gcttaaaatt tagttataga tcaagaaaaa
     4801 acattttttt ctaaaattct taatcgttac tatttattat aactatctaa cattaaaatt
     4861 aaatgagaaa aacctaattt ttcagataag tttctacact tataaaaaag attattaatc
     4921 cctttgttag tagtaagtta tacgtatatt ctaacacatc ttacattttt aagaaatact
     4981 gttataatga taattattaa aatattacta agaaagtagg catttaaatg gagacattat
     5041 tttcaggcat ccaacctagt ggaattccta ctattggaaa ttatattggc gcactaaaac
     5101 aatttgttga tgtgcaaaat gactatgatt gttatttctg tatcgtagat caacatgcaa
     5161 ttacaatgcc acaagatcgt ttaaaattac gtaaacagac cagacaatta gcagcgattt
     5221 atttagcttc tggtatagat ccagacaaag caacattgtt catacaatct gaagtcccta
     5281 cacacgtaca agcaggatgg atgttaacta cgattgcttc tgttggagaa ttagagcgta
     5341 tgacgcaata caaagataaa gctcagaaag cagttgaagg tataccagct ggtctattaa
     5401 catatccacc tttaatggca gctgatattg ttctttacaa tactaatatc gttccagttg
     5461 gagatgacca aaagcagcat atcgaattga ctcgtaacct tgtagataga tttaatagtc
     5521 gctataatga tgtgcttgtg aaacctgaaa ttcgtatgcc taaagttggt ggacgtgtca
     5581 tgagtttaca agacccaaca agaaaaatga gtaagagtga tgataatgct aaaaacttca
     5641 tttcattatt ggacgagccg aatgttgcag ctaaaaaaat taaaagcgca gtaactgatt
     5701 cagatggtat tattaaattt gatcgtgaca acaagccagg tataacaaat ttaatttcaa
     5761 tatacgctgg attaaccgac atgccaatta aagatattga ggcaaaatat gaaggggaag
     5821 gttatggtaa atttaaaggt gaccttgctg aaatagttaa agcattttta gtagaattcc
     5881 aagaaaaata cgaaagtttc tataactcag ataaacttga tgatatttta gatcaaggta
     5941 gagataaagc acacaaagtt tcatttaaaa ctgtcaaaaa aatggaaaaa gcgatgggtt
     6001 taggacgtaa gagataaaat aaagaggtac tagaattaac tagtgcctct ttaatttcat
     6061 attaattttt aatatctact tctttatcat ttttctatat atacatattt taatgttgta
     6121 tcaggcccaa ccacatgata ttgaagtcct tttactttgg gattggttaa atgagcttct
     6181 cccttttgat aaattggagc tagaggtgcc tcttttaaca ataactcttc atcttctttc
     6241 attttactat ctctttcgct aacgtcccct aataactttc catttatttc tcctaaataa
     6301 tcatcatatt ttttatttcc ccagtcagta ttattttgag gattatcttt tgtcattgtt
     6361 gacaaatagg ttgttggatc aggataatca gcagcccatc cagttaatga agattcatat
     6421 gtcattttaa attcttcact caatctctgt ttgaaaggta gttgtctaat tttcattttt
     6481 acaccaggta aatttttttc tatttgggcc tttatatatt ctgctgatat ttttgaatct
     6541 ggtgtatctt cagtattcat tgaaaatgtg aattttttta tattcaactc tttctttgct
     6601 ttttcccaat attccaacgc ttttttctta tcatacttta atggtgaatt tacacttgaa
     6661 gcataatcat caccattgct aagttttgcc acgcctttag ctgtgaatgt gtcactaaat
     6721 gtagaaccat cattcttaac tgcttcaaca tatccttttt tatcaattgc taatgcaatt
     6781 gcaatgcgta aatttctatt tttaaattct ttaacctttt tttgattgaa tttaatgaaa
     6841 aaattacctg aagttaagcg tttttgtaaa gcctttgaat tctcatattt ttttacttga
     6901 tcggctaaaa ttaatgtatc atcaacagat cctgtttcaa ataatgaagc accagcttgt
     6961 ttatctttca atattttata attaacttta tctaatttta cgttcttttt atcccaatag
     7021 aaattattct tagacaaaag tatcttatct tctgctctcc aagtatctac cttaaacggc
     7081 ccattataaa ctgcattttg aggtttcaaa ccatactttt ctccaaactt tttcgcaact
     7141 ttttcatttt gtggataaaa attacttaaa gccaacaatt catttatgta tggtataggt
     7201 cttttcaact ttatttcaag ttcatattta cttaatgctt taatacctaa cttttcaact
     7261 ggtaacttac cagcgtttat actatctgca ttttcaatat cagacattat gtaagcatat
     7321 tcagaacctg tttttggatc aactaatttt ctccatgcat atacaaaatc atttgctgtc
     7381 actgggtctc cgttagacca tttagcgtca tttcttaatt taaaagttaa agttttacca
     7441 ccattacttt ttttaggcag ttctttggct aacgctaatt ttgctttatc gttttcatca
     7501 agtgtaaaaa gcccttcgaa tacttgattt gatatatcac ctgaaactgc atctgttatt
     7561 aaagctgtat ctaaggtgct catatcttgt ggtattactt tcctaaaaac ttgtccttta
     7621 tcagagtata taccttcttt gctacaacca gacaatatca atacaaaaat aaaacttaat
     7681 aataagtatt ttactttaga aatattcata atattgtgtt ccctctttct aaaagtatta
     7741 gaatttccgt tgtctttaca tttctgcaac atttagactt gtgagtacat aatgatcatt
     7801 ttctattttt cgtaatttgc actcagaatc attatcaaat ttatcattgt aaatttctct
     7861 aactctttct ttttcatata ttggatctgg ttggggtatt gaagatatta gtgattttgt
     7921 ataattatgt aatggatttt gataaagcct ttctgcacta gcaatttcaa ctattttccc
     7981 tttatgcata actgctattc tatctgaaac atacttaacc atagataaat catgagcaat
     8041 aaataaaagt gttgtctctc tttctttttg caattttttt atgatgttta ttatttgtgc
     8101 ttgtatcgaa acatccaatg ctgatatagg ttcatctgct ataatcaatt ctggttccac
     8161 agataatgct cttgcaattc caattctttg tctttgtccg ccagaaaact catgtggata
     8221 ccgatttgca tgctctttat ttaaacccac taattctaat aatttataca ctttttcatt
     8281 tctttcatct ctatttttcg ctaatttatg tatgtctatc ccctcaccta ctatatccct
     8341 tacttttagc cttggattta aggaagcata aggatcttga aaaatcattt gaatctgttt
     8401 atttaattta aggctatctc ttctagatgt tattttttaa tatctttccc tttaaagaat
     8461 atagtttctt ctggtacatc taataatcta ataatggact ttccaaatgt agatttacca
     8521 cacccagatt ctcctactat cccaagcgcc tcacctttat ttatttttat acttacatca
     8581 tcaattgctt ttatttcatt gcttttattt ttattaaaaa ttttggttaa atttttaatt
     8641 tcaacaagta cattattttt catcaaatga tactccctcc acttttaacg gtctctcaaa
     8701 attataagga aggtttttaa ttttctcctt aattacttct ggtggaatta cttttggagc
     8761 cctgctatca agtaaccaag atttcacgaa atgtgtaggt gataattgaa accatggcgc
     8821 ttcatattta aaatcaatat ttaacgcata cgcacttctt cttgcaaacg catcaccagt
     8881 tggtggcttc aacaaattag gaggcgctcc tggaatagca atcagttttg actttgaatt
     8941 agactctaat gttggcattg atgcaagaag cccccatgta tatggatgtt ttggattata
     9001 aaataattct tctacttttc cagtttctac aatttcacca caatacataa ctgacactgt
     9061 atctgcaatg tttgcaacga ctcctaaatc atgagttata aaaataattg cagtatttgt
     9121 tttcttttgt aattttttca tcaattcaat aatttgcgat tgcatagtta catctaatgc
     9181 agtggttggc tcatcagcta ttaataattt cggttcacaa gctaatgcca taccaattac
     9241 aatcctttga cgttgtccgc cagaaaactg atgtgggtaa tctttaaacc ttaattcagc
     9301 attctttata cctactaaat ctaaaatctc tatagctttt actttggcat cttttttatt
     9361 catactttta tgtatcatta aaggttccat aatctgctta cctacttgca ttgtaggatt
     9421 tagtgatgtc attggatctt gaaaaatcat agctatatct tttcctcgta ttttagtaaa
     9481 ttctttttca tttttattaa ctaaatcttc tccgaaaaac accacactcc catttgtgat
     9541 tcttccatta tccccttgaa aaagtttagt aattgcttta gtagcaactg atttacctga
     9601 gcctgattcc ccaacaattg ctagtacttc tccctcattt atgcaataat caattcctct
     9661 tacagcatta acaatgccat cttctacttc gaaagaaact tctaaattct ttacttccaa
     9721 cacttttctt accattaaat cacaccctta tttacgcatt ttaggatcaa acgcatcgcg
     9781 taatccatca ctaaataaat agaaaaataa aatcaaaaga cttaaaattg ttgcgggtat
     9841 gaataactca tgcggataaa ttaaaagcat tgatttacca tcattcacta atgaacctag
     9901 cgatgtttga ggagcaggaa caccaattcc aataaaactt aaaaaagctt caaaaaaaat
     9961 tgcgcttgga actgtaaaca tcgaagtaac aattattgca cctaatgtat taggcaatat
    10021 gtgtttgaat attaatcttt tatttgaagc tcctaatgtc ttagacgctt gaacaaattc
    10081 taaattttta attttaagaa actctcctct taccactcta ctcattgata tccatccagt
    10141 gacagacatg gctaaaataa ttgtccaaat tgatggttca aaaattaata cgaataaaat
    10201 tactattatt aaattaggaa tcgaagctat aacttctata attctttgca ttacagtatc
    10261 tattttccct ccataaaatc cagatatagc accaaataaa acccctataa aaacatctaa
    10321 aatagctgcg accacaccaa taaataatga tatgcgtgca ccttcccaag ttcttgtcca
    10381 caaatctcta cctaattgat cggttccaaa tatgtaataa tctttagacc ccacctcttt
    10441 gtatgtatct ttgccgttaa catctttatc attaaaaggt aaataatcaa cttttcctaa
    10501 caaaggtatt ttcggaggta aatttctatc tgtaacttgc tgctcagtac tattatgttg
    10561 agaaattgca ggaccaaata aagcaaataa aattaagatt attaatccaa tcatacctat
    10621 aacagctaat ctattttttc ttaattgtgt ccaagcatct tgccaaaagc ttttattttt
    10681 aacatttaca aattcttgat tcatgttatc tttttttaat tcaaaatctg ttgaagtcaa
    10741 ttgcttatta ttttcatcaa tcagcattaa tttcttcctc cttgaatgcg tattctcgga
    10801 tcaataatta tgtataacat atccactaaa aatattgaca ctataaaaaa actactgaat
    10861 aaaatagtcg tagccattat tactggaaaa tcatttgtgg caatagaacg tacaaattga
    10921 tctcctaacc ctgggactcc aaaaatattt tcaatagtta aagtaccagt caatatacta
    10981 gccatcattg gtacaagtac tgtcacaagc ggaataagag catttcttaa agcatgtgta
    11041 tataaaatct ttttactaga aacacctttt gccctagcca acagtatata atcacttttt
    11101 aatacttcta aaagttcagc acgtatatat ctagtgactg tcgcaataac aatggcagat
    11161 aacgcaaaag aagggagtat aattgttgaa ataccttccc aaccagcaac aggaaaccat
    11221 ctcaatttaa ctgcaaatac aaattgtaaa ataactgcaa gtacaaaaga aggaacagat
    11281 actgctaata ccgatataat tgttgctaaa taatctatcc aagtattttg tttcgcagct
    11341 gaagcaactc ctaaaattaa tccaatcagc atccctatta tcattgctgt aatacccatt
    11401 tccaaagaag gaatcaacct tggcttgata atgtcccata ctggttggtt atgatactga
    11461 aatgaataac caaaatctcc tgtaaacaca tttttcatgt aatttaaata ttgaataggt
    11521 atagggtcat ttaatccata tttctcattt aaaatatatt tttgatctgg acttaattta
    11581 gcatcattaa aaggtgaacc tggcattaac ttcattagaa agaacgttat tgttgtaata
    11641 attactaatg aaattgccat ataaaaaatt ctcttcaaaa tatatttaat cattgcaatc
    11701 tatccactcc atttaactta attttctgaa tattaacact attatatatc tattttattt
    11761 attttcaata tttttttatt gcaggacatt agatttattt ttattcccaa agtttttatc
    11821 caacacaaaa agactggaga aagttctcca gcctcattta caaagcatat taatttttct
    11881 ttttaccggt ttctttatca attgatttat ctatatacac atttttaagt gagctattcg
    11941 gtccaaattt atgataaatt agtcctttaa cttggggatt ggttaaatgt gcggtacctt
    12001 tttgataaat cggtgcaact ggtgcatcat ttaggaatat ctcctctgct ttttttaagc
    12061 tttcatatcg ttctgtcggt tgaagcgcca attcgtttct tgcatctttt agtagttgat
    12121 catactcttt attactccag tccgtattat tttgtgcatt gcctgtagtc attgtttcta
    12181 aataagctat aggatctgga taatctgctg accatccact tagtgatgct tgataattac
    12241 cacttaactc taatgaaact ctttgcttaa atggcaattg cttaatcttc aaagtaacac
    12301 ctggtagatt tttctcaact tgcgatttaa tatattctgc agatattttt gcatctggcg
    12361 tatcttcagt gttcatagaa aatgtcactt catttttacc taattctttt ttagcttttt
    12421 cccaatgcgc tcttgcttct ttaggattgt attttaacgg tgaattcatt gtactcgcat
    12481 aatctttacc atttggtgct ttagcaatgc cttttgctgt taatgtatct gatggtattg
    12541 atccattgtt ttttactgaa tcaacataac cttttttatc aattgcttgt gcgattgcca
    12601 gtcttaattc tttattttta aactcaggat attctttctc gttcattttc acaaaaaatg
    12661 tcccagttga taacacaaag tttaaccctt tgttgtcttt atacttattc acttgatctg
    12721 cggtaatgag cgcatcatct actgattcag tatcatacaa tgaagcacct gcctgtaagt
    12781 ctttgataac cttataattc actttatcta actttacatt ctttttatcc caataatact
    12841 gatttttaga taataatatt ttatcttctt gtttccaatc gtcaacttta aacggaccat
    12901 tgtataccgc tctatctgct gcagtaccat aattttcacc atattttttg gcaacttttt
    12961 cattttgagg tgcaaatgta ttaagtgcta ataattgatt aatatacgga accggctttt
    13021 ctaattcaat ttgtaatgtt tcatcatcta atgctttgat acctaattgt tctacaggtt
    13081 tcttaccagc atttatatca ctagcatttt taatgtcacc cataatgtat gcaaattcag
    13141 aacctgtttt agggttaact gtttttctcc aagcataaac aaagtcttgc gcagtaactt
    13201 tgtcaccatt actccattta gcatcgcctc ttaatttaac ttttaacgtt ttaccgtctt
    13261 tacttttttc cggaaaagct ttcgctacac ctaacacagg tttgtcatct ttacctaacg
    13321 tgtataatcc ctcaaaagtt tgtgctgtca cttcaaaaga catttcttcc gttattaacg
    13381 atgtatcaag tgatgttaaa tcttgtggta aaatttttct gaatacttga cctttatccg
    13441 aataaattcc accatcatta gcacaaccac ttaatgcaaa tgtagcaatc aatattaaaa
    13501 taagtgttct aaattttctc gtcattgcat ttcccccttt cccttaattt tgtgtcacca
    13561 atttattttc tcggagttgt gccgcttctt cttcagtact aaagacaaag tgattcggtc
    13621 ttatttcatg taatcgtctt aaatgattgt tttcctcatc atcaatgtaa ctaaatcgtt
    13681 tgcgactgcg ttctgattca ggatcaggtt gaggaatagc tgataataat gacttggtat
    13741 aatcgtgtaa tggattttga taaatttctt cagacggtcc aatttcaact attttcccaa
    13801 aatgcattac tgcaatacga tctgaaatat acttcaccat tgatagatca tgagctataa
    13861 ataggaacgt gatacctctt tcacgttgta attttaataa caaattaact acttgagctt
    13921 gaattgaaac atccaatgcc gatattggtt catccgcgat aataaattct ggttccaccg
    13981 ctaatgcacg ggcaattcca atacgttgtc tttgtccacc tgaaaattca tgtggataac
    14041 gattagcatg ctctttactt agcccaaccg tttctagtaa atcatagacg cgtttttttc
    14101 ggtcacgttt atcacttgcc agatgatgta tatcaatacc ttcagcaaca atatccatca
    14161 cttttaatct aggatttagt gatgcatatg gatcttggaa aatcatttgg atttttttat
    14221 taaatttaag taagtcttta cgtttcttaa tcttttgtat atcagtaccc tcatacaaaa
    14281 tttctccact tgtaatatca ttaagcttaa taattgattt acctgtcgta gatttaccac
    14341 aaccagattc accaactaaa cctaatgttt cccctttgta tatatcaaac gaaatgttat
    14401 caatcgctct tacttcattt ttctttcctg cattaaaata ttgctttaaa tttttaatcg
    14461 atagtaatat ttcatcagtt ttcattgaac gacacccttt ctaccatgag tggtttttca
    14521 taattattag gcatcggttt catacgttgt tttaccagct cgggtagttc aacttttggt
    14581 gcacgtgcgt ctaataacca agatttcaca aaatgtgtcg gtgaaacttt aaaccacggt
    14641 ggttctactt taaaatcaat atctaatgca tattgactac gtctcgcaaa tgcatcacct
    14701 ttaggtgggt gtaataaatc aggtggcgct ccaggaatcg ctagtaatgg tgtgtcattt
    14761 gttgttgata aatcaggcat tgacgataat aatccccatg tatatggatg ctttggatca
    14821 taaaatattt cgttaacatc tcctgtttca accatttgac caccatacat aactgccact
    14881 ctatcagcaa tattcgcaac aacccctaaa tcatgcgtta taaaaataat tgctgtatcg
    14941 attttttgtt gtagttcttt cattaaatct aaaatttgtg cctgcatcgt tacgtctaat
    15001 gcagtcgttg gttcatcagc aatgagcact ttaggttcac aagctaatgc ggttgcaata
    15061 acaattcttt gcctttgtcc acctgaaaat tgatgtggat atgctttaaa tcttttttct
    15121 gcatttggta aacctacaag atttagtatt tccaatgcgc gctttttagc ttgtgcttta
    15181 ctataatttt tgtgcttaat taatggttcc atgacttgtt taccaatttg catagttggg
    15241 tttaaagatg tcattggatc ttgaaagatc attgaaatat ctttgccacg taatttaatt
    15301 aactcatttt caggtttttt tgctaaatct tcccctaaaa ataaaatttc tcccttttta
    15361 attcttcctg tgtccccttg gaataatttt gtaattgctt ttgttgttac agatttacct
    15421 gaacctgatt caccaacaat tgccaatgtt tcccccttgt ttaaataaaa atctacacct
    15481 ctcactgcct gcacttcccc tgctgtaata tcaaaggaaa catgcaaatc atttacttct
    15541 aatattcttt cagtcatatg ctatgccccc ttttatttac gcattttcgg atcaaatgca
    15601 tcacgtaatc catcactaaa taagtaaaag aatagaatta ataaacttaa aatcattgct
    15661 ggtataaata attcatgtgg ataaattaat aacattgctc gcccatcatt tactaatgac
    15721 cctaacgatg tttgaggtgc gggtacacct ataccaatga aacttaaaaa tgcttcgaag
    15781 aaaatagcac taggtactgt aaacattgat gtaaccacga tagcacctaa tgtattaggt
    15841 aaaatatgct taaatatcaa tttgaatttt gaagccccca atgttttcga agccatgaca
    15901 aactcttgat tttttaattt taaaaattct ccacgtacaa ctctgctcat gcctaaccag
    15961 cctgtgatag acatagccaa tataattgtc caaatggatg gttcaaaaat taatacaaat
    16021 aaaattacga caattaaatt cggaatagat gctatgactt caagtatacg ttgcataatc
    16081 gtatcgacac gtccaccgaa gaatccagaa atcgcaccat atacaacgcc aataaaaata
    16141 tctaacatcg ctgcaacaac accgataaac aatgaaattt gagcaccttt ccatgttctt
    16201 gtccataaat ctcgacccaa ctgatcagta ccaaaccaat aattttcttt agcatttgct
    16261 gctttataag catccttgcc atctgcatct ttaccatcaa aaggtaaaaa tggaactttg
    16321 tctaatacag gtatttttgc cggaagattt ctatgttcta cattttgttc agcataatca
    16381 tgtttattta taactggacc gataaaagca aatattacaa tgataattaa acctatcata
    16441 ccgacaacag ctaacttatt tcgttttaac tgagcccaag catcttgcca aaagtttttg
    16501 ctttctcgtt gcatttcagg ttcctgattc aaatctaatt ctctaataat aaaatcagat
    16561 gatgcgatag cgtcagaggt atgcgtcata gctgcattag aatgatcgtc gttaatcgac
    16621 aaattgtttt tattttcagc cattattttt tacctccttg aacacgaatt cgtggatcta
    16681 tcacaccgta caaaatatct acaataaaaa tagaaacgat aaacagtgtg ctaaataata
    16741 gtgtgattgc catgattact gagaaatcat ttgttgtaat tgaacgtacg aattgatccc
    16801 ctaatccagg aaccccaaaa atattttcaa ttgttaaagt acctgttaaa atacttgcta
    16861 acatgggaac gataattgta ataattggaa ttaaagcatt tctaagtgca tgtccaaaaa
    16921 gtacacgcat tgtcgaatta cctttagctc tcgctaataa aatatagtct gaacttaata
    16981 cctctatcat ctctgctctt atgtatctgg cgacagttgc taaaacagct gcagataatg
    17041 caagtgacgg taataccgcg gtcgaaaaac cttcccatcc agctactggg aaccatctta
    17101 atttaactgc aaatacatat tgtaaaagta cagcaagtac aaaagatggt acagatactg
    17161 caataactga aataactgta gttgtatagt caacccaaga attttgttta gtagctgctg
    17221 caacacctaa aataagtccc agtatcacac cgatgaacat tgctgtaaga cccatttcaa
    17281 aagaaggtag tagtctcggt ttaatcaaat cccacacagg ttgattatga tactggaatg
    17341 aattaccaaa atcgcctgta acaacatttt ttaaataatg taaatactgc gtagctacag
    17401 gatcatttaa tccatatttt tcatttaaaa tttctttttg ttcagcattt aatttagcat
    17461 cgttaaatgg cgaacctggc attaatttca ttaagaaaaa tgtaattgta ataataataa
    17521 ataaagaaat aagcatataa ataaatcgtt tgaaaatata tttccccatc taatcccctc
    17581 ctc
//
LOCUS       DAHHYJ010000048        16962 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000048 DAHHYJ010000000
VERSION     DAHHYJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16962
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.048"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            17..2422
                     /gene="mnhA1"
                     /locus_tag="NBK22_001183"
     CDS             17..2422
                     /gene="mnhA1"
                     /locus_tag="NBK22_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCD2414377.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWVWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGALILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            2415..2843
                     /gene="mnhB1"
                     /locus_tag="NBK22_001184"
     CDS             2415..2843
                     /gene="mnhB1"
                     /locus_tag="NBK22_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCD2414378.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            2843..3184
                     /gene="mnhC1"
                     /locus_tag="NBK22_001185"
     CDS             2843..3184
                     /gene="mnhC1"
                     /locus_tag="NBK22_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCD2414379.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            3177..4673
                     /gene="mnhD1"
                     /locus_tag="NBK22_001186"
     CDS             3177..4673
                     /gene="mnhD1"
                     /locus_tag="NBK22_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCD2414380.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            4675..5154
                     /gene="mnhE1"
                     /locus_tag="NBK22_001187"
     CDS             4675..5154
                     /gene="mnhE1"
                     /locus_tag="NBK22_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCD2414381.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            5154..5447
                     /gene="mnhF1"
                     /locus_tag="NBK22_001188"
     CDS             5154..5447
                     /gene="mnhF1"
                     /locus_tag="NBK22_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCD2414382.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            5425..5781
                     /gene="mnhG1"
                     /locus_tag="NBK22_001189"
     CDS             5425..5781
                     /gene="mnhG1"
                     /locus_tag="NBK22_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCD2414383.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            6030..7184
                     /locus_tag="NBK22_001190"
     CDS             6030..7184
                     /locus_tag="NBK22_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD2414384.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     YTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKELDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSSFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISGGVINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(7240..7614)
                     /locus_tag="NBK22_001191"
     CDS             complement(7240..7614)
                     /locus_tag="NBK22_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCD2414385.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(7633..8949)
                     /locus_tag="NBK22_001192"
     CDS             complement(7633..8949)
                     /locus_tag="NBK22_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCD2414386.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGVVSGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILVVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     MMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
     gene            complement(9360..10835)
                     /locus_tag="NBK22_001193"
     CDS             complement(9360..10835)
                     /locus_tag="NBK22_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCD2414387.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKARSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAVNVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSKVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(10966..12174)
                     /locus_tag="NBK22_001194"
     CDS             complement(10966..12174)
                     /locus_tag="NBK22_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2414388.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(12628..12987)
                     /locus_tag="NBK22_001195"
     CDS             complement(12628..12987)
                     /locus_tag="NBK22_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCD2414389.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEVLEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(13000..13236)
                     /locus_tag="NBK22_001196"
     CDS             complement(13000..13236)
                     /locus_tag="NBK22_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCD2414390.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            13553..14617
                     /locus_tag="NBK22_001197"
     CDS             13553..14617
                     /locus_tag="NBK22_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2414391.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(14676..14999)
                     /locus_tag="NBK22_001198"
     CDS             complement(14676..14999)
                     /locus_tag="NBK22_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCD2414392.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            15100..15342
                     /locus_tag="NBK22_001199"
     CDS             15100..15342
                     /locus_tag="NBK22_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCD2414393.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERALHEEVPGVIEVEQVF"
     gene            complement(15605..16780)
                     /gene="dltD"
                     /locus_tag="NBK22_001200"
     CDS             complement(15605..16780)
                     /gene="dltD"
                     /locus_tag="NBK22_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCD2414394.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTSLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(16777..16959)
                     /gene="dltC"
                     /locus_tag="NBK22_001201"
     CDS             complement(16777..16959)
                     /gene="dltC"
                     /locus_tag="NBK22_001201"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCD2414395.1"
                     /translation="MVKENPDVEIFEEGIIDSFQTVGLLLEIQNKLDIEVSIMDFDRD
                     EWATPNKIVEALEELR"
BASE COUNT         5377 a         2945 c         2635 g         6005 t
ORIGIN      
        1 aaaggaggac aacatagtga gtttgttaca tattgcggtg atattaccgt taatattcgc
       61 actcattatt cctatcctgt atagattttt taaacgtata catttaggat ggttcgtatt
      121 acctgtccct atcgtcatat ttatttatat gcttacatta attaaaacga cgatgtcagg
      181 taatactgta atgaaaacct taaattggat gcctcatttt ggtatgaact ttgatttata
      241 tcttgatggc ttaggtttac tctttagttt gcttatttca ggaatcggca gtttagtagt
      301 attatattca atcggctact taagtaagtc agaacaacta gggaattttt attgttattt
      361 attactattt atgggtgcaa tgttaggtgt cgtactttca gataatgtta ttattctata
      421 tttattctgg gagctaacgt cattttctag ttttttactt atttcattct ggcgcgaacg
      481 acaagcatct atatatggtg ctcaaaaatc gctcataatc acagtatttg gtggtctatc
      541 attattaggt ggtatcatct tactcgctat accaacgcaa tcatttagca ttcagtacat
      601 gattcaacat gcttctgaaa ttcaaaatag cccattcttc atttttgcga tgattttaat
      661 catgattggt gcttttacaa aatctgctca gtttccattt tatatttggc taccagatgc
      721 aatggaagca cctacaccag tcagtgctta tcttcattca gcaacaatgg ttaaagcagg
      781 gttatattta atcgctcgaa tgacaccaat cttcgcagca tcgcaaggtt gggtatggac
      841 tgtcacactc gttggtttaa ttacgttatt ctgggcttcg ctaaacgcaa caaaacaaca
      901 agacttgaaa ggtatactgg cattttcaac agtatcccaa ttaggtatga ttatggcgat
      961 gcttggaatc ggtgccatta gttatcacta tcaaggcgat gatagcaaaa tatatgctgc
     1021 tgcatttacg gctgcaatat tccatttaat caaccatgca acatttaaag gtgccctatt
     1081 tatgattaca ggtgcagtgg atcattctac tggtacacgt gatgttaaga aattaggtgg
     1141 cttactaaca attatgccta tttcatttac aatcactgtt attacagcat taagtatggc
     1201 tggtgtacca ccgttcaatg gtttcttatc aaaagaatca tttttagaaa caacatttac
     1261 agcaagccaa gcaaatttat tcagtgttga tacattaggc tacctattcc caatcattgg
     1321 tatagtgggc agtgtcttca catttgtata ttcaattaaa tttataatgc atatattttt
     1381 cggacaatat aaacctgaac aattacctaa aaaagcacac gaagtatcaa tattaatgct
     1441 tttatcacca gctattttag ctacattagt aattgtattc ggtttattcc ctggcatttt
     1501 aactaattcg attattgaac cagctacatc ttcgattaat catacggtaa ttgatgatgt
     1561 tgaattccat atgttccatg gattaacgcc tgccttttta tcaacattag tgatttatat
     1621 tttaggtatc ttactaattg tgacatttag ttattgggtg aaattgttgc aacgacaacc
     1681 tggtaaacta acattcaatt attggtacaa cagaagtgca aatgttattc caaactactc
     1741 tgaaaagatg acgaatagct atgttaccga ttattcacgt aataatttag ttatcatttt
     1801 tggtgcatta attttattaa catttgtcac tatattctca gtacctttca acattaattt
     1861 taaagacgtt agtccgatta gaattttcga agtttgtatc gtaattttat tattaagtgc
     1921 tgccttttta attcttttcg ctaaatcaag attatttagc atcattatgt taagtgcagt
     1981 tggatatgct gtgtctgtat tgtttatatt ctttaaagca cccgacttag cattgacaca
     2041 gtttgtcgtt gaatctattt caacagcatt gttcttatta tgcttctacc acttaccaaa
     2101 tttaaatcgt tataatgaaa agcgctcatt ccagcttact aatgctttaa ttgctggtgg
     2161 tgttggtttg tccgtcatta ttatcggttt aattgcatac ggaaatagac attttgaatc
     2221 gatatctaag ttttatcaag agcacgttta tgatttagct cacggtaaaa acatggtaaa
     2281 cgtcatactt gttgatttcc gtggtatgga tactttattc gagtcatctg tgcttggtat
     2341 cgcaggatta gctgtatata caatgattaa attacgtaaa aagcgtcaaa cgcaaggtaa
     2401 cgaggtgaaa aatcatgaat agacaacaaa atgatctaat tttgcaattt gctgcagtta
     2461 ttatcttttt catggtaatg gtctttggtt tttcactttt cttagcagga cattatacac
     2521 ctggtggcgg tttcgtcggc ggattattat tcgcaagttc acttgttatc attacaattg
     2581 catttgatat agaaacaatg cgtaaaattt tcccgttaga ttttaaaatc ttgataggta
     2641 tcggattagt tttttgtatt gcgacgccta tcgcaagttg gtttttaggc aaaaatttct
     2701 ttacgcatgt cacatttgat ataccgttat ttatattaga acctgttcat atgacaacag
     2761 cagtattctt tgatttcggt gttttatgtg cagtagttgg tactgtcatg acaattatta
     2821 tttcgattgg agagaacgaa tagtggaaat tattatgatt tttgttagtg gtattctcac
     2881 agcaattagt gtctatctcg ttttgtctaa aagtctgata cgaattgtta tgggaactac
     2941 actattaaca catgcagcaa atttattttt aataactatg ggcggactta aacatggtac
     3001 tgttccaatt tatgaagcga acgtaaaaag ctatgttgat cctatcccgc aagcacttat
     3061 tttaacagca atcgttatcg cctttgcgac aacagccttt ttcttagtat tagcatttag
     3121 aacatataaa gaattaggca cagataacgt tgagagtatg aaaggagttc cagaagatga
     3181 ttgaaagtaa tatgcttgtg ttaacgctag ttattccagt aattacagcc atattacttg
     3241 tctttattgg aaagcgtcct attataaagc gttatgtcgc actaggcggt acattattaa
     3301 ccttggtcgc ggcaattatc aatttagcaa atgttgttaa acatggacca atacgcgtag
     3361 agctaggttc atggaaagca ccatacagta ttgtctttgt attagacata ttcagtgcgc
     3421 tactaataat tactagtatt attattacag ctattgtgat tttatattca taccaaacaa
     3481 ttggtattga acgtgaaaga tattattatt atttttcagt actatttatg ttaataggta
     3541 tcattggtgc attcacaact ggtgacattt tcaacctatt cgtatttttc gaagtatttt
     3601 taatgtcatc ttatttctta ttagtaattg gttctacaaa aattcaacta caagaaacaa
     3661 taaaatatgt tcttgttaac gttgtatcgt catccttctt cgtaatgggt gtagcgattt
     3721 tatattcagt agtaggaaca ttgaatctag cagatataag caataaatta gctaaccttt
     3781 cagcgcatga cagtggctta gtcaatatcg ttttcatact atttatcttt gtttttgcta
     3841 caaaagctgg cgtattccca atgtttgttt ggttacctag tgcatactat gcaccaccaa
     3901 ttccaatcat tgcattcttt ggtgctttgt taacaaaagt tggtgtttat gcaatcgcta
     3961 gaacattgag cctattcttt agtgataatg taagcttttc acattatgtc attttattct
     4021 tagcactatt aacgatcatc tttggttgtg tcggtgctgt tgcatatgcc aacattaaaa
     4081 aaatcatcct atacaatgtc atgattgctg taggtgtgat cttagtcggc gttgcaatga
     4141 tgacagagtc aggtatgatt ggggctattt actatacatt gcacgacatg ttagtaaaac
     4201 ttgcattatt cttgcttatc ggaattatga ttaaaattac aggcactgct gatttaagac
     4261 aatttggtgg cttaattaaa cgatatcctg tacttggttg gtcattcttt atcgcagcat
     4321 taagtttagc tggcattccc ccactcagtg gcttttatgg aaaatttttc attgtccaat
     4381 caacatttga aagaggattt tatttaagtg gtgtcattgt cttattatca agtttagtcg
     4441 tattatattc tgtcattcgc atattcctac aaggcttttt cggtcaacca aaaggatatg
     4501 atcttaataa caaagtcgac gtcaaatatt taactacgat tgcaatcgtt gcagttgtga
     4561 taactgtttt atatggattg tcagcagatt acttatatcc aatggtaaaa gctggtgcag
     4621 aaacattcta caatccatca acctacgtta aagctgtatt aggaggtaaa taacatggct
     4681 gtacaattag tattaaactt tattatcgca gtgttttggt tgtttgtaac aaatagttac
     4741 acaacaaata actttgtgtt aggtttcatc tttggcctag ttcttgttta cctcttacac
     4801 agagtgttac caggtagatt ttatgtcatc acgctatatc gtattattaa attagttatt
     4861 attttcttaa tcgaactgat taaagctaat tttgatgttt tgaaaattat tattaaacct
     4921 tcgattaaaa atgaaccagg attctttgtt tatcacacag atttaaaaaa agattggcaa
     4981 attgtcttgc tgtctaattt aattacatta acacctggta ctgtagtgct tggtgtaagt
     5041 gacgatcgta caaaaattta tattcatgcc atagattttt caactaaaga gcaagaagta
     5101 gaaagtatta aaacatcatt agagaaaatt gtaagagagg tgggcgaaat ataatgaatc
     5161 ataatgttat tatcgttatt gcattaatca tagttgtcat ttctatgtta gctatgctca
     5221 ttcgcgttgt gctaggccca tcacttgccg atcgtgttgt cgcattagat gcgattggtc
     5281 ttcaattaat ggcagttata gcattattca gtattttatt aaatattaaa tacatgattg
     5341 tcgttattat gatgattggt atattagctt ttttaggtac tgcagtattc tctaaattta
     5401 tggacaaagg taaggtgatt gaacatgatc aaaatcatac tgattagtct tgcacttatc
     5461 tttgttatca tcggtgcttt aattagcgcc ctagcagcta taggattatt gagacttgaa
     5521 gatgtatatt cacgtgcaca tgctgccgga aaagcatcaa cattaggtgc aatgtcatta
     5581 ctatttggta cgtttttata ttttattgct actcaaggtt ttgtaaatat gcaattaatc
     5641 gttgcgatta tctttgtatt aattacaggt cctctttcaa gtcatatgat tatgaaagca
     5701 gcatataata ttaaaacgcc ttatactaaa aagactaaag tcgatgaaat ttcggaagac
     5761 ttaaaagaca caaaattgta gattatataa caaaatcatg aatataaatc aacaacaaac
     5821 agcagtaaga tgattccaaa ttaggaatga ttttactgct gttttctttt gacattgtta
     5881 cctctttttc aatgattttt tctttgacta cagattcgcc ctatctacat atatctcttt
     5941 aatttaattg cctttcatgt cgttatgtat tatgataata ataattataa atcgtaacga
     6001 ttacgtttta aaaagagaga ggttttatta tgcattggac aattatcggc ggtggcatac
     6061 agggaactgc aatcgcacaa aaactattat caagcggatt aacaacagac cgattaacaa
     6121 tcattgaccc acacgaaact ttttgccaaa ggtttaactc atatacaaat cgaatagaaa
     6181 tgccttattt aagatcaccg attgtacatc acgtacatcc acaaccgttc catctaaaac
     6241 aattcgctaa acagcaccaa tatacaaatg ctttttatgg tccttatcaa cgacctgaat
     6301 tgacaatgtt tatggatcat attgcacatg cttctaaaca atatcaatta gaggattgct
     6361 tggttcaagg tttagttcaa actttagata aacaagaaga caaatggcat atcaagttag
     6421 aagatggaca aattatcact acagattgcg tcgttattgc aataggcagt acaaatattc
     6481 cgtttatgcc tgacatttta aaagacaaac agaatgtaaa tcatatcttc gagaaagaac
     6541 ttgatcaagt agtatatgat aagaccgatc atatcgttgg tagcggcatt actgctgcac
     6601 atcttgcact taaattgtta aatcatgata acgataaaaa gattcattta tggctaaata
     6661 aagatattga aatacatgac tttgatgctg atcctggttg gttaggtccg aaaaatatgt
     6721 cttcattttt aagtactaaa agcatgcctg aaagaaatgc cattgtacaa cgcgaacgtc
     6781 ataaaggatc aatgcctcac gaactgtact tacgccttaa aaaacatatt aaaaatggtc
     6841 gtataaatgt gcataaaaca cctatcactc aaattagtgg tggtgtaatt aacactgaaa
     6901 atgattctgt tccatatcaa cagattatgg ttgcaactgg ttttgaacaa gattttatgt
     6961 cacaaccact tattaagcaa ttaatacaaa attatgatgc acctatcaac gaatgtaatt
     7021 accctgttat ttccgaaaaa ttagaatgga taccaaatct atttgtcgca ggatgctttg
     7081 cagacttaga attaggacca tttggtagaa atgttatggg tggccgtaaa gctgccgaac
     7141 gcattgaaca agcatttcta aaactacaac aatatagcgc ataaaaaaac tgctagccgt
     7201 agttatgaat atctcataac atttcagcta gcagttcttt tattttaaag gtttaatagc
     7261 aactgtacca cgcataactg taattaattg ttctttgtca ttcttaattt ttatatccca
     7321 tacatgtgtc gacttacctc gatgaataat ttcagctgtc gcagtaacac gaccatcttt
     7381 agcagaatga atatggttag cattcatctc taaacctaat ggaataaatt tggttgtatc
     7441 aattaaatta gcagatccta atgaacatgc tgtttcacct aaagcaatcg aagccccacc
     7501 atgtaaatat ccaaatggtt gttttacttt atcagtaaca ggcatagaaa taacaactaa
     7561 accatcttcc tggtgatcta ttgacatctc aaatgtctct aataaatgag tcatattttc
     7621 acctctttat gcttatagta ccatagcagc aatagtaccg aaaatcatta aaggaatatt
     7681 ataaaacaag aagtttggta cacatgtatc acgtatatga tcatgttggc catcaacatt
     7741 taatcccgca gttggtccta atgttgaatc acttgcaggc gaacctgagt cacctaatgc
     7801 actcgctgtt ccaatcaatg cgattaatgc cattgtatct agtccaattg acgctccaaa
     7861 aggaatgaat aatgatgcga taataggaat tgttgcaaat gatgatccaa tacctaaagt
     7921 gacaattaaa cctatcacat acatcatgat aatgctaaat aatttattat caccagtaat
     7981 acttgttaaa gttttaacta attcatctat atcaccagta gcattcatta caccagcaaa
     8041 tccatttgct gttaaaataa ctacaccaat ataagccata attttaatac cttcaacaaa
     8101 cttagcatct aattcatacc aattatatgc acgtgaaata aagaatacga gtacccctgc
     8161 cagtgcacca aaaatcattg aatctgtaaa tgtttgtact aaaaatgtag ctagtattgc
     8221 tacaattgtt actactaaga tatatggttt taactctgta acattgtcac tatctgaaat
     8281 tttacgtgtt tcatattcac gtggtttacg atatacatat aaaccgataa gtaagccaac
     8341 aatatacccc attgaaggaa taagcattgc tttccaaatc atattaaact caattgggtg
     8401 atttgccttt gcaaagccac tttgaataat ttgctggaaa atttgaccga atccatatgg
     8461 taataacaca tacgggaaac ataaaccaaa accgataatc aaaccgatta aacgtctatc
     8521 tatttttaag tcattaaaca gacttaacaa tggtgggatg acaattggaa tgaatgcaat
     8581 atgtacagga attaagtttt gactcatgat actcatagct aataatgcaa taattattgt
     8641 cacttttact ttaactcttg accatcgact attttcagcg tgtattgcat ttataatttt
     8701 tcctactaaa tagtctgtga taccactgta tgaaattaat gctgcaaatc cacctaataa
     8761 ggcatagctt aatgctacct cagcaccatc gactatattt tttccgaata cattaattac
     8821 tttttcaatg ctcatacctg atactacgcc accaactagc gcactgataa ataagcttat
     8881 aactacgttt aatcgacata aacatagcac aatcattaaa attactgcta ttactactgc
     8941 atttatcata tgacaaccat cctttatcac tctatcgaac taaagcgttt atagactaca
     9001 tagtatcagt agctttaaca cctgtcaata gcaaactata ccaataatct ttatttttta
     9061 gttaattgtc tttcttaaaa taattttagc tttcattaaa ttaaacaatt ttacaagctt
     9121 ggaacaccaa tcaaaatcct aagttctaaa atgtaatatt agtagtcgtt gactgaatga
     9181 acatatgctt ataatatttt tttgcaatgc tagtcaagtt gatttatgct cacaaggata
     9241 tgcgatttat attttcttac aacaatgaaa atgcctgata caatgcgatc ctttatactt
     9301 tcaagagtta aattagaaat gtatatcaag acacaagttg tatcaggcaa ataattaaat
     9361 tattgttgtt ttaaccattg tactatcgtc ggaatcataa atcctgttgg cccttttgga
     9421 ccattatatg aagctttatt agtcgttgct ggacctgcaa tatcgaaatg aatgtgaggt
     9481 gtttgaccac taaaatgtgt tacaaaactt gccgcaaata gcgctttacc ttgtccattc
     9541 gtatggttaa ctaaatcagc gatatcactg tgtttaatac ttgcacgttc ggttgcagta
     9601 atcggtaatt caaataccat ttcatcgact ttagaactta tttgtaatat atcgtttaat
     9661 atcactttac tattcgattc aaatgcagca gctttatcat cgcctagtgc aacaattgct
     9721 gcacccgtta atgtagcaaa gtccataatc acactaggct gatattgatt tgcataatac
     9781 acagcatctg caaggactaa tctaccttca gcgtctgtat tcattacttc tacagtttca
     9841 ccacttaatg ctgtaaatac atcatctggc ttcattgatg cttcatttat catattttca
     9901 gcacaagcaa gcactccgac aatatttaca ggcagttgta aacgactagc cgcttcaatg
     9961 ataccaacga cattcacagc gccacacatg tcaaacttca ttgtagccat gccattcttc
    10021 gttttaatac tataaccacc agaatcatac gttatacctt taccaactaa ggcaattggt
    10081 gcttcatctt tgtctttgcc attatatgtg atggttacta atctcggttt atgcttacta
    10141 cctttaccta ctgcttgtaa aagtccgaat ccttcagaaa ctaaagtgtc ataatcttta
    10201 acatctactt tgacctttgt atttttaaaa tgattaacaa tatcttctgc aaatgtttgt
    10261 ggtgttaata cattcggtgg catattacta aagtctcttg ccaaattaat ggattggcca
    10321 atactgatac cctcatgaat gaaatctaat tcaattaatg attcactaat aaggtttaaa
    10381 ttcgtcttaa acggtgcctt cttacttgat ttataatgat cgaattcata tgttgcacgc
    10441 tcactttgaa taccgcatgc cattaataca tctgataact gatcatattt tgaaataaat
    10501 gaatccataa gtaaataagt atcttcaatg tgttctgact ttatgtattg gaaaagatgt
    10561 ccccatattt tcaacatatc ttgataacta cgtgctttta agtttcctaa accaactgta
    10621 attaaacgat atgtttgatc ttgtacatca aatgcagttg tataaatctt cccaacttta
    10681 ctaccaataa tatgttgatg ttttagtctt tcaagtgatt ctgtaatatc gatatgatta
    10741 aaactaatgc gctctaactg atttaaatgt tctggtatac caataatcaa tgtatttatt
    10801 tcgttgctta gtgtgttatt taatttaaaa ttcataatgt acctccttag atttgatatg
    10861 ttactcactt caaattgtac aacaataaag cccttcagtg acactgaagg actttataaa
    10921 tgaataattt aattttatgt gtttagctga aactttattt aaagcctaga atttaccttt
    10981 tttgaatgct aaaccgatac caccgatttt gaataccgca cgtgtatcaa tcactttttt
    11041 catgaatgct gcttttttac cagcgatagg tttaccaaat accataccta caccgtcatg
    11101 tgaacctaaa gaacaaacag ttccacgatc aacgtattcg aattcttcag ttgattcacc
    11161 gtttaagatg cgtttaatgt ttttagcaac actttcacct tgttgcattg caatttgtgc
    11221 tgtagttggt aatggacgtt cttctccagc tgggataaac gctgaacagt caccaataac
    11281 aaaaatgttg tcgtaaccat tgattgttaa atcttgcttt gtaacgatac gtccacgttt
    11341 aacgccttca aatgattctt ccattaattt actaccacgt acaccagctg cccatactga
    11401 agtacctgca tttaattgtt gtttttcacc atctacttca actacaaaac ctttttcgtt
    11461 acaagcaacg attggtgtag caattttaaa ttcaacaccg cggtcttcta agtagctaac
    11521 tgcgtggtta actaattctt ctgagaacat tggtaacatt ttaggtgctg cttcaacaca
    11581 agtgatttta actttatttt gatccacacc atatttgcta cataattcag gaattctgtc
    11641 tgttaattca cctaagaatt caacaccagt gaatccagca ccaccaacta agatagataa
    11701 atcgttatca tctttttctt ttgatgctgc atagttagca aatttgtctt cgatatgacg
    11761 tgataattca cgtgctgtga taacattttc aatttggaaa gcatgatctt tcataccttc
    11821 gatgccgaat gtttcactaa cgaaacctaa tgctactact aaaatatcaa agtcataaat
    11881 accttgattt gtttctacct ttttagcatc acggtcaatt tttgttactt ctgcttgaac
    11941 aaagttcact ttgtctttct tcaagacact ttccacagga tataatacat cttcatagtt
    12001 tagtgtacct gctgatgctt catgtaacca tgttgcttca tagtgatatt catttttatt
    12061 aataagcgta atttctgctt cttctgttga tatcgctttt tgcaatttag ttacagtttg
    12121 taaacctgca taaccagcac caagtacaag tactttttta cgatcttgag ccatttaatt
    12181 tcacctaagc tttcatattt ttttaaccaa atgctgataa ttactagtaa caactggtca
    12241 ttactacttt taccacaatg tatactagtt tatcatcatt ttgttattta agtcttttaa
    12301 attatgtaaa aacctgtgat gttcacaaaa aagacatttt taatccaata ctaattctat
    12361 agcttttatg ttatattttc aagcaagatt cagtcagatt gtgaaaaaat taattaattt
    12421 tttcgtagcc tgatttcgat taaaaatgta tccgttatca cgatcattta aaatactatt
    12481 tatagtgaga ttcttaattt tcaataagct ttttaataaa acaaaatatc tttacgcaaa
    12541 aataggacat aatcaaatat gtccctaatc ataattgata tcaatttgta tcccaaaaat
    12601 tgggttaacc attaaaaagt tattttatta gcaattttca ggattacctg caacttttgc
    12661 agttctaaat gaactaccac agccacatga agcaattgca ttaggattgt cgatttggaa
    12721 accgccaccc attaatgatt gcttaaaatc aatagtcgta ccatttaata cgggtgcatc
    12781 ttttttgtct actaaaactt ttaatccaaa gtattctaag acttcatcat tttcaccagg
    12841 cgcttcttct gcacccatac cgtatgttaa accagtgcac ccgccaccat tcactttaat
    12901 ttttaaatag ccatctggca tttcatttgc tttaagcata tcttttactt cgtaagcagc
    12961 tgcttctgtt aatataactg ttggcatgat aactcctcct taaaaaatcc aagtttcttt
    13021 tatatgtgca tatatatttt gtaataattc ttccggcgaa tcaccttcaa caatatcacc
    13081 atttactaaa gcatacaacc cggctgaaca tataccacaa tgtgtcaggc aaccatactc
    13141 taacacatca acatctgggt cattttccag ttgattaaaa acataatctc caccttttgc
    13201 catgttagag agacaaaatt ctacgatcgg attcatactt caccttctta tttcatttgt
    13261 tacaatatta tagcatttta aaactggtat tttaacatga tgtgctcaat tagcaacaac
    13321 tgatgtttct tatcccagtt atgtaatagt gccttagtta gtacaaatat ttatttgtag
    13381 tcattttcaa aaaaatatat aataagcata taaatacata tttacttttt taggggttat
    13441 tgatgaatca aagacacttg cattttataa ctgttcatta acaaatatat ctatgcactt
    13501 tgatcttttc aaaattaaag taaatattaa catataaaag gggtacttca ttatgaaaaa
    13561 cttagttttg ttaggcggcg gatatggtaa tatgcgtatc atgtcacgca ttttaactac
    13621 ttctttacca caagattata cagtcacatt agttgatcgt atgccatttc atggattgaa
    13681 accagaattt tatgctttag ctgcgggcac gaaatcagat aaagatgttc gcatgaaatt
    13741 ccctaatcat ccacaagtga atacagttta tggtgaaatt aacgacatag atttagatgc
    13801 tcaaattgtc tcagtcggta attctaaaat tgattatgat gagctaatca ttggtttagg
    13861 atgtgaagat aaatatcata acgttccagg agccgaagaa tatacacata gtattcaaac
    13921 actctcaaag gctcgggata ctttccatag tattagtgaa ctaccagaag gtgctaaagt
    13981 tggtatcgtt ggtgctggat taagcggcat agaacttgcc agcgaattaa gagaaagtag
    14041 atcagacttg gaaatatatc tttatgaccg tgggccgcga attttaagaa attttccaga
    14101 aaaattaagt aagtatgttg cgaaatggtt cgccaaaaat aatgttaccg ttgttccaaa
    14161 ttcaaatatt aataaagttg aacctggtaa aatatataac tgtgatgaac ctaaagatat
    14221 tgatttagtt gtatggacag caggaattca acctgttgaa gttgttcgta acttgccgat
    14281 tgatataaat agtaatggac gcgtgatagt taaccagtat catcaagtac caacatatcg
    14341 taacgtctat gtagttggtg attgtgctga tttaccacat gcgccaagtg ctcagttagc
    14401 cgaagttcaa ggtgatcaaa ttgccgatgt gcttaaaaag caatggctaa atgaaccatt
    14461 acctgacaaa atgccggaac taaaggtaca aggtatcgtt gggtcattag gagataaaca
    14521 aggttttgca tatatcatgg atcgcactgt aacaggacga ctcgcttcta ttttaaaatc
    14581 cggcgtactt tggttatata aatatcataa tggttaaaat taaaaagcaa tgtcagttca
    14641 cactattact gacattgctt ttagttatgt gtatattatt cattcacaag cttttggtct
    14701 ataaatcgag tgatttgctt tgtttgtata taaccatctg ctacatattc atcgttcatt
    14761 gtaattaatg gataaaatag ttcatcttgt tctattcttt caataaattg taaatcatga
    14821 tctgttaagt tgtcattatc ttttgtaata tctatatacg tatatttaaa tgatatattt
    14881 gggtattttc tttttaatag cggctgtagc cagtcataaa tatctttcga tgttggcgca
    14941 ttaacgcaac ttgcacatat aacatctgcc ccatatacta ccacactcac gtgctccata
    15001 tatttatccc ccattggttt gatagatttt tattacacaa tctattataa tatactaata
    15061 aagattatca aattcatctc tcgaaaggag acttgcctga tgcctactga agatacaacg
    15121 atgtttgatc aagtagcaga agttattgaa cgtcttcgtc catttttatt acgtgatggt
    15181 ggcgactgct cattgattga cgttgaagac ggtattgtta aattacaatt acatggtgca
    15241 tgtggtacat gcccaagttc tacaatcact cttaaagctg gtattgagcg tgcattacac
    15301 gaagaagtgc ctggtgtaat agaagtagaa caagtattct aatataaaga tattaagtta
    15361 acagaacata ttatgaataa caacaaagac aggaatactg aattatttat aatcagttat
    15421 tacctgtctt ttttcgctgt caaattggaa tgatgggaaa gttattttat actatttatc
    15481 tgattaatta ttttattatt gccacatatc caagcacata ttactattct attctatgaa
    15541 atttaaaata taaaaatagc acatacgctc aatcaaaatc gttgagttat gtgctatttg
    15601 tattttaatt tttaggttta tctacttcag gttgtggttc acctttcata tgtttcgcaa
    15661 tttgctcatc catataaacc caacctttcc aaccgatgtg tacggcatca ctgataacat
    15721 atttttcata atctttatca gtcatatcgt aaattttacc accattatct acaacagtag
    15781 aatggatttt tttataaact gcttgacgac gttctttatc gataccaatg tggtcatacc
    15841 atacaccgtt tgatggaata cttacatatt gaacatcagc accagcagca cgcattgttt
    15901 ttacaagtaa ttctaaatct tggaattctg gagaattaac attgaattcg tagtcacgtc
    15961 taactttacg cttactttct tgaattaatt tccagtattg atctctaata ccaaatttat
    16021 tcgatgtagt atcagcttta ccaatttcaa ctgctttttg tttcatctca tcccaagaag
    16081 cgtctggttt tgtagcaggt ttaacatgtt ctaatggaga tttatccatt gcaaacaatg
    16141 atttaatcgc ttctatttta atcaattgat tctctttaaa accagaaata taactatctt
    16201 tagtttcttt agggttttta gcataagatt tcaagtattc tttattgtgt acatgtggaa
    16261 actgtaataa acgttgtgca taacgacgtt ttaattcagt agacatgttt ttctgctgga
    16321 acatttgatt aatttgagtt tgagacatac gagcatcaaa gttttgattc gttaaaccat
    16381 ggtttgtaaa ccattgtggt gaaataataa atgttaattt cttacctttt aatttatcat
    16441 actgtgatgc aagttcaact gcgttaatta agtctgtaga accaccagca cctaataaga
    16501 atgctttttt gttggcgtta tgcttattta atgcaattgc aggattaaat gggtcatctt
    16561 tacctaattc actagagccg tatataggat aatacttgtt tgattcgtat aacttatctt
    16621 gaatgagtgt gccttttagt acttgatctg tcaatgaagt tctattatct tctacagtct
    16681 tttcatttac taatcctgta aaccaactag caggtaataa tagaaagaca atgaataccg
    16741 ctccactaat taaaatgggt aaaaaaggtt ttaatttcat cgtaactctt ctaatgcttc
    16801 aacgatttta tttggtgttg cccactcatc tctatcaaag tccataatag atacttcgat
    16861 atcaagttta ttttgaatct ctaataataa tccaactgtt tggaaagaat caataatacc
    16921 ttcttcaaaa atttctacgt ctggattttc ttttacaata tc
//
LOCUS       DAHHYJ010000049        16955 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000049 DAHHYJ010000000
VERSION     DAHHYJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16955
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.049"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            92..583
                     /locus_tag="NBK22_001202"
     CDS             92..583
                     /locus_tag="NBK22_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD2414396.1"
                     /translation="MIRCAKKEDLNAILAIYNDAIINTTAVYTYKPQTIDERIAWFET
                     KQRNHEPIFVFEENGSVLGFATFGSFRPWPAYQYTIEHSIYVDASARGKGIASQLLQR
                     LIVEAKAKGYRTLVAGIDASNEASIKLHQKFNFKHAGTLTNVGYKFDYWLDLAFYELD
                     LKD"
     gene            complement(727..897)
                     /locus_tag="NBK22_001203"
     CDS             complement(727..897)
                     /locus_tag="NBK22_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414397.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(959..1057)
                     /locus_tag="NBK22_001204"
     CDS             complement(959..1057)
                     /locus_tag="NBK22_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414398.1"
                     /translation="MIKKKHLSGSFVPDRCFLIIFHKVEKFMGNAC"
     gene            complement(1076..1975)
                     /gene="sdaAA"
                     /locus_tag="NBK22_001205"
     CDS             complement(1076..1975)
                     /gene="sdaAA"
                     /locus_tag="NBK22_001205"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCD2414399.1"
                     /translation="MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(1989..2669)
                     /gene="sdaAB"
                     /locus_tag="NBK22_001206"
     CDS             complement(1989..2669)
                     /gene="sdaAB"
                     /locus_tag="NBK22_001206"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCD2414400.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(2672..3715)
                     /locus_tag="NBK22_001207"
     CDS             complement(2672..3715)
                     /locus_tag="NBK22_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCD2414401.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
     gene            4265..4468
                     /locus_tag="NBK22_001208"
     CDS             4265..4468
                     /locus_tag="NBK22_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414402.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            4651..5586
                     /locus_tag="NBK22_001209"
     CDS             4651..5586
                     /locus_tag="NBK22_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCD2414403.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPETLDE"
     gene            complement(5668..6636)
                     /locus_tag="NBK22_001210"
     CDS             complement(5668..6636)
                     /locus_tag="NBK22_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2414404.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVTVDKHYGSTVYTVNDKQDKHQRVV
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLYPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(6769..7113)
                     /locus_tag="NBK22_001211"
     CDS             complement(6769..7113)
                     /locus_tag="NBK22_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD2414405.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEH"
     gene            complement(7155..7565)
                     /locus_tag="NBK22_001212"
     CDS             complement(7155..7565)
                     /locus_tag="NBK22_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD2414406.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
     gene            complement(7931..9997)
                     /locus_tag="NBK22_001213"
     CDS             complement(7931..9997)
                     /locus_tag="NBK22_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCD2414407.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVVLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGIGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKAHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(10298..12037)
                     /locus_tag="NBK22_001214"
     CDS             complement(10298..12037)
                     /locus_tag="NBK22_001214"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCD2414408.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAVYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELRAFVEAAKIPVVHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQDADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(12084..12773)
                     /locus_tag="NBK22_001215"
     CDS             complement(12084..12773)
                     /locus_tag="NBK22_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCD2414409.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(12766..13161)
                     /gene="cidA"
                     /locus_tag="NBK22_001216"
     CDS             complement(12766..13161)
                     /gene="cidA"
                     /locus_tag="NBK22_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCD2414410.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(13374..14252)
                     /locus_tag="NBK22_001217"
     CDS             complement(13374..14252)
                     /locus_tag="NBK22_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2414411.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            14419..14637
                     /locus_tag="NBK22_001218"
     CDS             14419..14637
                     /locus_tag="NBK22_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD2414412.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            15027..15458
                     /locus_tag="NBK22_001219"
     CDS             15027..15458
                     /locus_tag="NBK22_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD2414413.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNRWGNAKNWYYNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(15597..16874)
                     /locus_tag="NBK22_001220"
     CDS             complement(15597..16874)
                     /locus_tag="NBK22_001220"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCD2414414.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKQEPRANTQAAERILQEIRQQ"
BASE COUNT         5251 a         3294 c         2550 g         5860 t
ORIGIN      
        1 atttgcacat atattgtcac gttagatttc attacacata tttgcattca aataacgaaa
       61 cgtttttaat aattactaag ggggaattat tatgattcga tgtgctaaaa aagaagattt
      121 aaacgctata ttagcgatat acaacgatgc cattattaat acaacagctg tttatactta
      181 taaaccgcaa accatagacg aacgtatcgc atggtttgaa acgaaacaac gtaatcatga
      241 gcctatcttt gtatttgagg aaaatggaag tgtattaggg tttgcgacgt tcggttcgtt
      301 tagaccttgg ccagcatatc aatatactat cgagcattct atttatgtcg atgcttcagc
      361 tagaggaaaa ggtatcgcta gtcaattatt acagcgttta attgtagagg caaaagctaa
      421 aggttatcgt acacttgttg ctggtattga tgcttctaat gaagcgagta ttaaattaca
      481 tcaaaaattc aatttcaagc atgccggcac actgaccaat gtaggctata aatttgatta
      541 ctggttagat ttagcttttt atgaattaga tttaaaagac tagtagtgtt taaatcacat
      601 aatataaaca agacaaccat gttaattcac ttaacataac atgctaacat ataaaactat
      661 aaacctctta gggacaattt acatcgtccc ttttttcttt tcaaatacta tccccatagc
      721 tttgatttaa cgctttttct caataacaaa acgaatatag tagaacatga aaacgataat
      781 catgctgagc gataaagatt taaataatag attgacccac gttccctcag tcgtatatcc
      841 atatgtaatc gttgtgttaa tgatgaatgc tataaagatg attgatagtc ttagcatatc
      901 atcactcctt ttaagttatt ttagatatac gggggcgctt ttgcaatcac tattttgatt
      961 agcatgcatt tcccataaat ttttcaactt tatgaaagat aattaagaag catctatctg
     1021 gtacgaatga tccagacaga tgcttctttt ttatcaatat cttattgtta tcttattaat
     1081 tatttttaac catatcttca gctgtgccaa agattttacg tttaattgct tcgcctgttg
     1141 gtgtgcctgc tagtccaccc aatccagttt cacgtaatga tgcaggaagg ttacgaccaa
     1201 ccttatccat tgcttcaata acttcatcaa cagggattct actttcaata cctgctaatg
     1261 ctaaatctgc tgaaattaaa gcgttacccg aaccaattgc atttctcata acacaaggaa
     1321 tttcaacaag tccggctact gggtcacaaa ctaaacctaa taaattactt atcgctaatg
     1381 ccatagcgtg cccggatgct tctggtgatc ctccgaatat agctactgct gcagctgccg
     1441 ccattgcaga tgctgaacca acttcagctt ggcagccacc tgttgcacca gctacacttg
     1501 cattgtttgc tacgacacgc ccaaacaatg ctgaagtgaa taagaaatca atcatttgct
     1561 cttctgttaa gtcatgtgtt ttttctaatt taaaaagtgc accgggaatg gtacccgagg
     1621 aaccagctgt tggcgttgca caaataatac ccatcgcagc attgacttca tttgttgcaa
     1681 tggcaccttt gacggcatca atcatttcat atccagacaa agcatgatgt gtttcattat
     1741 aatcacgtag tttagcagcg tcatgaccag tgtagcccgt tacactttca acgccatcac
     1801 ctgtcgtccc tttgattact gcgtctctca tgacatctaa attttgtttc atttgcgctc
     1861 gcacttcatc acgtgattta ccgcttaatt ccatttcttc tttaaccatg atatccgcaa
     1921 atgacatatt attttctacg gcataatcta tagtctctct aattgaatca aacatgttta
     1981 ttccccctct aatttatata ggaaacgttt acgtcactgt atttctcttt aattgtattt
     2041 aatgtcgatt ctgagattgc tttatttaat ggtattacaa ccaagcattt atcttcatct
     2101 atcttaataa attcatcttt acagtctaat ttcatatcgt tgatatcatt aatgaaatga
     2161 tttacttgtg ctttagtcat atttccgtca acaactaaaa ttggtaatcc atgatttaaa
     2221 tctacttcta gtccatttat atgaatacct ttaattttaa ttgtaccacc accgattgaa
     2281 ataccgataa tttcaatata gcgaccatca ttacgagatg atttaatata agcacagttt
     2341 ggatgttgac caatactatc gccttcttct tcgatgatat ctattttaat accatcatca
     2401 gctgcaattt ctaatgaaga tttaattcgg ttatcaaatg ttgaatatcc cattgctcca
     2461 cccacaatag cgacatctgt accatgtcct tggtgtgttt gagcaaatga ttcataataa
     2521 tgtatttcaa tatttttagg aatatctccc aatattgcgc gtgctgaatt cccaatcttt
     2581 actgcaccag ccgtatgaga acttgaaggg cccatcataa ctggtccgat aatatcgaaa
     2641 gcactttgat aatcatagct ctttgccata attaaacact ctccttaata tgattctttt
     2701 tgctcggcat tttaaagttg atattcatta aattaaactt attaattagt gttccaataa
     2761 tataggctaa gacaatgctg acaatgatca ctgttgcgat tgttacaatt gatgtcactg
     2821 cattattaaa gccaaacaat acgatggcac ctgcaattgg tgttgccata cctttgacac
     2881 ctattactag tccgctaaat gtcacgatac atgcgttgac gaccccaatc agtgcatttg
     2941 taccatatag ttgtactgga tattgcgcta ttaaatcaat ttgcgtcaat ggctcaatac
     3001 aaactgcaaa tgcttttgac ggtccaccaa tgtttaattt tcggaataaa ataaggttaa
     3061 caaatgagct acctgtacat gttagtgctc caatagccat aggaacacct gtcagtccta
     3121 ataaacttgt taataccatt gaacttagcg gtgtcatacc tgtaacagga atcactagtc
     3181 ctaaaatgac cgctaatgca tatggattgt tatcacctac cgcagtgaca gcactaccta
     3241 tttgttttaa tgttgctagc acaccaggtg taatgattga tgcaagtccg aaagcaattg
     3301 ctggtgcaaa taagatcacc acaattaagt ccaagccttc tggaactttc ttttcaatcc
     3361 atttaattaa aaaagctacg ccataagctg cgatgaatgc tggtaataat ttaaagtcat
     3421 gtaatactaa accaacaatg accgcaaata ctggtgcaac gcctaagttt aagcacgtta
     3481 gaatacctac tgcgataccg cttaaacttc ctgctaaatc cccaatatct tgtagaaatt
     3541 taatatcaaa tacgccacca atagcataac ttaagaatgc ttgtggtaga aatgtcgcac
     3601 aagctgcacc ggataatgct tgtagtcctt gtttaccgta cggtgcatac tttaaaaata
     3661 gcgtcatgat cactaaaacc aagactaatg tgcctacacc taacagaata tccatttccc
     3721 aaaaacctct ctctatgttt attttatttt cagaccataa acatcgtaca cccttaagaa
     3781 aacgttttca acttttatct gttatcaaat caaatattta agtgaaatat ttcatatttg
     3841 tgaaagattt ttaaaatgga ttgtttcaaa aactacttat gttgtcgtta atatttacta
     3901 attaactttt tactctatat ttcaaacagt tgtgtgacag ttttttgata acttttttac
     3961 atctgaaagt aagtaatatt tctaaaaact tttaatattt atacacttta tctttcgagc
     4021 tatttatttt ttgtttttgt gctgtgttta taaaaccgca cactatattc aaaaataact
     4081 tattacacat atatttgcaa taagataatt aaagtaggat attattttta gttttctgat
     4141 aggaatgatg atagtctata ggttgaatct ttactttttt taaagctaaa tttacatcaa
     4201 cttaacaatg gttggttata ctgaagatga aaatatttag tataacttag tggaggcgat
     4261 aaaggtgcaa ttgagtcatt ccgttaaagt tgcaatttct atctatttag cacttatctt
     4321 tataacgttc acttcttatt tagtcattat tttatatacg agtatgactg gacatgatgt
     4381 atcacatttc gtgttagata gtcagcattc tcatcatgga tctcttacgc aaaaacattt
     4441 gagtcttcct gaaatctcat ttaaatagtt tatcctctgt gtttcaacat tcatttccca
     4501 tatcgattca tttatctatc atctagacca ctacatctta gatgattttt ttattttctc
     4561 atttcactct ttcttaaaag tcgatataat gaattaaatc attatcatac accgacatat
     4621 tttatgttat tggtgttaag ttttaaaggg gtgagatact tggcgaataa tcattcagct
     4681 ttgcgtttaa aaggaattat acttgccatt gtcggtgctt gtttatgggg attaggtggt
     4741 actgtttctg atttcttgtt caaatataag aatattaatg tcgattggta cgtcactgct
     4801 cgacttgtag tcagtggtgt tttcttactt attatgtaca aaatgatgca acccaaacgt
     4861 tcaatattta gcgtattcca agatcgacgt atgttaggca aattacttat cttcagtata
     4921 ctgggcatgt tagtagtaca atatgcttat atggcatcta ttaatacagg taatgctgcg
     4981 attgcaacat tactacaata cattgcgcca gtttatatta ttatttggtt tgtcataaga
     5041 ggcgttgcaa aactaacatt atttgatgtg cttgctatta tcatgacact attaggaaca
     5101 tttttattat taacaaatgg ttcattttct aatttagtcg tcaatcctgc aagtttattc
     5161 tggggtattt tagctggtgt agcactcgct ttttacacaa tttatccttc agacctactt
     5221 aaccgcttcg gttcgattct aattgtcggg tgggcaatgc ttatttctgg tgttgcgatg
     5281 aatttacgcc atccaatttg gcacattgat atcactaaat gggacatatc aattatatta
     5341 tttttaatct ttggtattat cggtggtacc gcactcgcat tttatttctt tatcgacagt
     5401 ttacaataca tatcagcgaa agaaacaaca ttattcggaa ctgttgaacc tgtcgtagcc
     5461 gttatcgcaa gcagtctatg gttacatgtg gcattcaaac catttcaaat cgtaggcatc
     5521 attcttatta tgattttaat tttattacta tcacttaaaa gacaacctga aacattagat
     5581 gaataagaaa actctgataa tcactttagc aagtaactat tatttaacaa cgtagttacc
     5641 ttataggtga tatcagagtt ttttatttta gttaataata tttttcactt ggtataaaaa
     5701 gcgtcgtcgc tctggtatcg gaaatactgg ataaatatgg aattggtaat atccaggtat
     5761 aaagtcatgt tcgatacctt ttgcactcaa caattgcgat aagttcaaag catctgggta
     5821 cagtacttct tttgtgccaa cagttaatgt aatacgacct aaaccatcta agtcaccatt
     5881 aattggtgat actttgtagt tatctaaagg tgtgtcccct gcccattgtt cagctaagaa
     5941 cacactgcca tccacaccta ccattgggtc tttctttaag tagtcaggaa tttcaggatg
     6001 ctgcatcgtt gcatctaaaa ctggtgaaat taatacaata tgtcctggtt gcacaatatg
     6061 tttttctttt aacaattgag caaatgataa agcaatttga ccgcccgcag agtcacccat
     6121 tacaacgatt tgtttagaat ctgctacttg attcaataaa tcatggtata acttttcaaa
     6181 aagcacatac gtcgcttgat aatcttgatg cggaatcttc ggatatactg gcatgatgac
     6241 tttagcattg agtgtttctg caagttcatc aataaattcg aagtgaattt taagtgggtc
     6301 ttggaaccat gcgcctccat gtgcgtataa tacaacacgt tgatgcttat cttgtttatc
     6361 attaacggta taaacggttg aaccgtagtg cttatccact gttactggca tcttaaactg
     6421 ataattggtg ccctcatatt cacctgctgt ctgtggcgcc attttttcta atgctttcat
     6481 cgcttcttct ttatacataa aacgacgctt agctctagat aatcgtatac cttgttctaa
     6541 catataactt ttcacactgc gtttttcttt aattctaata tgtgcgtatg ctgctactaa
     6601 aaatccaaac gcaagtgtag accatttttt acgcatttgt cgtcactcct ctttgatgat
     6661 ttctactata gttctactgt accatgctga ttcttattac taccaatatt tactgggaaa
     6721 taagttatga tgtctacata atcttagcaa tgctaatcat ttgcgatatt aatgctcatc
     6781 gacatcgtta tttacactat cattcatttg cttaagatga tgttcaaaac gttgcatatc
     6841 gataaaacgc ccttgacgat gcatttcttt tccattcata aaaattaaat ccacaggtac
     6901 agtgaaaata tttaattctc cagcaatagc ttccacctga ctttgattaa tcacagctaa
     6961 tggcacattg ggatatgatt gcatcaagtc ttcaatttgt ggtaaaacgg catgacacac
     7021 gctacactga tcgcgcatga catgtacaac tgctaacgga tattgatgaa taaattcacg
     7081 gaatgcttcg acactattta attgcttaac catttcgaac accttcttac tttttatatt
     7141 ttaaaatgaa gtcattattt tgttgctaaa atgtcttgaa acacttgtgg cacagaggtt
     7201 atcttcttat cgctcaatct gtaaaaaccg cctgtctgtg taccaataca atgcacgcca
     7261 tgtgcggaca caatttcaaa ctgaaacacc catttcatct tgcttgcttt aactaagtaa
     7321 atacgaccta ctggtttgtc atgtattgtg acaggtactt tatactctgc ttccgttttc
     7381 ataagtatag gtgaaatgcc ttgattgatc attgttgagt aattcatgtg ctgattaata
     7441 aacgctgttc tcaaatcttc gaaccatcta atataaacga tattactaac gacacccatc
     7501 gcatcaatgt cgtaaccatt cacttcgatt tctttctctg caactaatag accttctgtt
     7561 gtcataatga caaatctcct tatcttacaa atagatttaa aatcatgtta atttattttt
     7621 attactttca tgataaaaat acaagcaatt gaactttatt acggaaaatc aaacttttca
     7681 atgacgttat taatttggct agtcgtgaag atgcattttt ctaaaatgta caaaaagtac
     7741 aatccctact ttctatcccc caaactaaca tctatgccat tgctatacat cattgcgcac
     7801 taaaaagaca gcagcttcat tttaagctac tgccttttgt aaaaatattc tctttcaact
     7861 cgctacttcg catacgcact acgaatgact aaaagttcat tcatttgata tgctgtttca
     7921 tttttaatca ttatttgact gtcataatta gcttacctgg atcagcatct tgtacatctt
     7981 caatgacaag ttctttattt tcaagatttg taataatcat tggtgtaacg atgcttggtg
     8041 cgtgcgcttt caagtatgct aaattcactt tcattaatgg ttgaccttgt gttacttttt
     8101 catcaacgtt aatcagactt tcgaatcctt caccattcag tttcactgta tcgataccaa
     8161 tatgaataag tacttcgaca ccactttcag attctaatcc tatcgcatgt ttcgtaggga
     8221 agattgtttt cactgtacca tcaaacggtg ctacaatttc acctttttca gggataaagc
     8281 caacaccatc acccatcatt ttaccagcga atacttgatc aggtacttct gataatggaa
     8341 tgatttgacc gatacctggt gcatagatgt ctgttgttcc aagtgcttct acgtgaactg
     8401 gttcatctga catttcttca gtcactgtcg tttcacttgg tttcgtaatt tcaccactca
     8461 taatcttggc catatcatgt ttaatttgat ctgattttgg accaaagata gcttgcatat
     8521 tgtttccaac ttctaataca cctgatgcgc ctaaagcttt aatacctgct acatctactt
     8581 ttgatttatc aaccacttct acgcgcagac gtgtaataca tgcatctaaa tgtttaatgt
     8641 tttcttttcc acccattgca tctaagacat caaatggtaa ttttgcgaca ctagagttac
     8701 gaatttcagt ttcttcatct tcacgacctg gtgttttcaa tttaaactta cgaattgcaa
     8761 agtcgaataa gaagtaatac acgatagcat atacaatacc gactggaata actaataatg
     8821 cgtgtgaacg atcccagttt aataaaccat ataaaatata atctatgaaa ccacctgaga
     8881 atgtcatacc aattttaacg cctaataaat gcattactaa gaatgatgta ccagctaata
     8941 atacgtgaat tccataaagt actggtgcta caaataagaa tgaaaattct aatggctcag
     9001 tgataccagt taaaaatgca gttaatcctg ctgataacat taaaccaccc acgacttttt
     9061 tgcgttctgg tcgtgcattt ttataaatag caaatgctgc cgctggtaaa ccaaacatca
     9121 taaatggata tttaccagta gtaaatgcac cagcagtaaa tggtacgcca tctttcaatt
     9181 gtgccatcca aatacgttgg tcacctcgaa ctaattcacc tgcgtgattt gtataacttc
     9241 cgaattcaaa ccagaatggt gaatagaaaa tatgatgtaa accaaatgga attaatgagc
     9301 gttcaataat accgaatata aacgttgtta atgttaaatt tttatttaat aagaaattcg
     9361 ataaactatt taatccatct tgaattggtg gccacgcaaa gcttaaaacc acacctgttg
     9421 caattgctac gaccgatgtc acaatcggta caaatcgttt acctgcaaag aatcctaaaa
     9481 atggtggtag tgtaatgtta taaaatttgt tgtagcacca tgctgctaaa gcacccatga
     9541 taataccgcc aaacacaccc gtttgtaacg ttggaatacc taatactaaa gcatgtgctg
     9601 gttctttcgc tgcttgactt aattcttttg cccctttggc atatgagaaa atgtcatcaa
     9661 ttgtaatgtg caacactttc cccattgttg cattcataat taagtaacct actagcgctg
     9721 ctaatgctgc aacaccgtct cctcctgcta atccaagtgc tgtaccaact gcaaataata
     9781 atggcaagtt atcaaataca acttgtcctg ctgcttccat gaccgacgaa atcattacaa
     9841 tgatatcgtt ttttaaccat ggtgcaattt ctactaattg ttcgttgtgc attgcgttac
     9901 caaacgctaa taaaatacca gctgctggta aaatcgcaac aggtaacatt aatgctttac
     9961 cgatacgttg caattgtcca aacaatttct taaacatata aacatcccct atctcaattt
    10021 ccgcttccat gtacaaaaaa agcaggcatg aacaaacaag actattctca tagtgcggtt
    10081 gtttgctcat gcctgcatta ccagtaacac gcaagtgata tctaattcat agctattata
    10141 catatttgta aaagcgtttt caacaacttt ttcacaattt tatcatcaat tcagtacgat
    10201 catttgtgat aaataaacaa aacggcaatt cccattttgt tgaaatagaa attaccgttt
    10261 ttatagttca attcaattgt gataaccttt caatcagtta taagaaacgt tttgctgcca
    10321 ctgaaattgg tggaatttga tctaaatcta aatgtttatc ttcagtaatt gatctaaatg
    10381 cccacttacc ataaccaagc gcttcttcat ttacaatttt acctggtaat ggcgcagcat
    10441 taggatcaac atatacatct acaatcgttg gtacatcttg tgctaatgcc tcttcgacta
    10501 tagcatctac ttcgctagca ctcttaattg tataaccttt accacctgct gcctcagcaa
    10561 attttgcatg atccatatca gaaaaatcaa ctgcatattc taattcacca gctgcttgtt
    10621 gttcatattt aataaatgct aactgtttgt tattaagtac aaatacagtt aaaggtaaat
    10681 catattgtac tgctgtagcg aagtcttgca ttaccatttg gaatgcaccg tcaccagcaa
    10741 tggcaatcgc ttgtctattt ggatatgcaa tttttgatgc catagctcct ggaagaccgc
    10801 accccattgt acctaaccaa cttgaaatga tgaacttgtt gttcacacca agatttaaat
    10861 aacgtgtaga ccaaactgtt gcagtaccaa catctgctga aattactgca tcatctttaa
    10921 taaatttgtt aattgatgcc attaaacgtt ctggacgtaa tggtttgcta ttattatttt
    10981 tatcttgttc catccattta tcccaaaccg ctttacgttc taacgttttg tttaaaaatg
    11041 gtctttccgc aacatgttta atattttcag ttaactgatg caatgcaatt ttactgtctc
    11101 caacaattcc tacattaata ttaaaacgat gtccgatatt tttaggattt gtgtcgattt
    11161 ggattgcttt aatatttttc ttaggtaagt aatctacata tggatagttt gtacctacca
    11221 taattagtaa atctgcatcc tgcattgtct gatatgaggt ttttgtacct attttaccta
    11281 agttaccaat actgtatgga tggtcatccg gtaagattgt cttagcaggt aatgaatgaa
    11341 cgaccggaat ttttgccgct tcaacaaacg cacgtagctc atctttcgca tgttttgcac
    11401 caacaccaat caacataacc ggctttttac tcttattgat taatttaaca gcttttttaa
    11461 tgtctttata ttttggtgat acaacagttg gtcttgatgt atctactggt ttattcgttg
    11521 tatctttaat tttttcagtt aataagtcgt taggacaaat aacaacagct acaccttttt
    11581 gttcatatgc cgtacgaatt gcttcgttaa caatttcaaa cacattgtca cctttttcaa
    11641 tttggtgatt ataaacggct acatcttcac ataatttttg taaatttgtt tcttggaatg
    11701 ctttcgttcc aagtgctgta ctattcgttt gtccagataa tattaattgc ggtacattat
    11761 ccattttggc atcatacata ccatttaata aatgaattaa accagggcca ccgatactta
    11821 atgccacacc gattttacca gttaattttg tgtaaccagc agccgctaag cttgctactt
    11881 cttcatgacg tacatgataa aatttaaatt gatctctcac tgtacgtaaa ctatcgacta
    11941 ctgcgtcgat tgagtctcct ggaataccat acaagtgatc tatatcccat gcttgtaatg
    12001 ctttaactaa tgcttcattt gcttttattt ttgccattat tactaatagc ctccctttct
    12061 gtctatcatt ttaaatatct aaattaatag aataataaaa ttagaacagg tattaacact
    12121 gaactaataa ctgcagttaa aatcatccca attgaactaa atgcaccgga ttcaatatcc
    12181 atttctagtg ctttagctgt gccaaatgca tgtgacgcat tcccatacgt taaacctttg
    12241 gcgatagaag attcaaatct tccaaatctt aataacatcg aacctaaaat actaccgatt
    12301 aaacccgttg tgataataaa aagtacggtc atcgtatctg taccacctag ttcatgtgac
    12361 acttcgatac ctactgcggc tgttatagat cggggcaata acgttacaat gacgtcttta
    12421 gaatagccaa atgctttaag tgttaagaac actaacatga aattcagcat gacgccagtt
    12481 aatacacttg caaaaatgat agagacattg tctttaattt tctctctatt tttataaagt
    12541 gggtacgcta aacatacgac cgttgcgttt aaaatatggt tgatccaact gccacctttc
    12601 atatacccgt tataactaat tccaaagata agtaagacaa aaataattcc taaagatgca
    12661 attaatgctg gattcaaaaa tgggttcgga tatttttgtt gtaacctttt agcgaaataa
    12721 tataaaacga cagtcaacaa aatcattaat aaggcttgca cgtaatcatt cataagcgtc
    12781 tacaccttta cgatgtttat gtttaacaga cattttttca gcaatataac ctgaagataa
    12841 tgcaacgata catgttccta taataatgac tgcgaaaaag agtatataat ttagcgtaat
    12901 ttcggaagca acatccataa tacctacaac tgacggtatg aagaaaaaga ccatcgtctt
    12961 taataaaaag tttgcaccgt cttctaccca agttaacggt acaatcttaa attgtaatag
    13021 taaataaaat aaaaatagac caacaatact gccggctaag ggaagatgaa aaatcttttg
    13081 aatttctgtg ccaatataag taatcacaat gatgattcct agttgtagta gtagtttgat
    13141 tattaattgg actttgtgca tggtgccatc cctttctaaa tatgtctaaa ttgttacaat
    13201 aactattata aagatggcgt acacaatata aaaatactct ttttttatag tttgtattac
    13261 tattttctat ggaaacgctc tctaaatttc acttcatttt cacaaaaaaa cactcattat
    13321 ttcaatgacc cacgtaatga tgcgccttaa ataatgagtg ctttattaat atgttagcct
    13381 aaacgatctt tcaaaaattc tatccatttc gttgtggcat gacttaattg tttatccttc
    13441 ttccaaacaa cacctaattc ccaatgtaca tgcgcatctt caatgcgtag tagcttcaca
    13501 tctccattta gttgctctga aattgatgtt ggtaaaatac taatacctaa ttcattcgta
    13561 actaaatctt ctataacatg ccattgtgaa atttgcgcta cagtattcgg aacaaagcca
    13621 acgtttttag cattttcaat aattttatca ttcaagtaaa agtctttatt aaataaaatg
    13681 aagtcttcac ctgctaaatc ttcgagttta acagtttcat attttgcgag tcgatgctct
    13741 ctgctcacga taagtcgcaa atcttcctta tctagggtag tataatcgaa aatatgatga
    13801 tcgactggca aagtggtcac gcctatatct acttcatcat tgataatttg ctgttcaatt
    13861 gttttaccgc cattttctat caaattatat gtaacatttg gatattgttg atggaatgca
    13921 ccaagaatat tgataagaat cttcatattc atgactgccg acatgcccat gtttatatgt
    13981 cctgtttcca gtccattcaa gcgttccatt tcagatggta aataatcata cagtgcaaca
    14041 atttctttac ttttctcata aaaaatttga cctgcatcgg taagaattaa atgtctttta
    14101 cttctatcaa ataagggcgt gcccatttca ttttcaatat ctttaattgc tttactaatt
    14161 gtaggttgtg caatatataa tgatttggaa gcattagtca tgcctccttg cttaacgact
    14221 tcaataaaat atttcatatg tttgatatcc actaacatca tcctaactat ttcaacacgt
    14281 ttatttcatt atacacaact tcttaaacat tcaataaatt tagatttata aaattgcttt
    14341 ataaaaagtt ttatgtaaaa tacacattct tttatggtat tatattaatg gctcaagtat
    14401 attaatcgag gtgaggtatt gagcttttac gaatttatgc aaagttttct tggtgatgac
    14461 acaccattag gtgaattggt tgattggatt aatcaagata gcaatttccc tagagaagtg
    14521 aagagccata gtgaaatatt gtcatatttt cgaacaaatc catgtcctga aagcatctca
    14581 gtacctgtaa ttaaacgggc actatcagtt ttcaaccaat tcacaaatgt acaatgatac
    14641 atgaatacat ggagtcacat catctcatgt tagcgctctg attgcagggc gctattttta
    14701 atgttattgc tacatttagt atattgtgat tgctattttc tttcagttat agaaccacgt
    14761 accttttctt ttaaatcatt ttatattttc ccactaatat ccgctgttaa tcaatcctga
    14821 catccttgta tcactatgac aattaattgt taaatacatg aatttctaca ttttatgaaa
    14881 aaatccattt ttattacaat tcaacacttt atatgacaac ttcattacag ttacttttat
    14941 tgttgattgc ttgcattgtt ttctaaaaaa attttgttat cataattaac gttgaataaa
    15001 gaaaaaaatt aacttgggag ataaaaatgg aatataaaaa gatactaatt cgtttattaa
    15061 ttgcttttgc agtacttttc tcagcagatt tcacttatca atccgtcgaa caaacgcatc
    15121 aatcgcatgc cgcagttaat tattatagta aaaaccaatg tacatggtgg gcatttaaac
    15181 gtcgcgcaca agtcggtaaa cctgtttcta atagatgggg caatgctaaa aattggtatt
    15241 acaatgcacg taaatcaaaa tatgcgactg gtcgtacacc aagaaaattt gctgtcatgc
    15301 aatcaactgc aggatattat ggacatgtcg cagttgttga acaagtatat aaaaacggta
    15361 gtattaaagt ttcagaatac aacttttatc gcccattaaa atacaataca cgtgtactaa
    15421 gcaaaaaggc agcacgtaac tttaactata tttactaatc aaaaaacttc tatcacgaac
    15481 gcttcaattt cctgtatgcg tgtgatagaa gtttttattt tttgttcata ttaatttatt
    15541 taagcgctac gatggactga cttaaacttt tctttaaaat cgctattcgc aatttactat
    15601 tgttgtctaa tttcttgtaa aatacgttcc gctgcttgtg tatttgcacg gggttcttgc
    15661 ttcaatgctt cagctacttt agcaatttca tcaccttttg ctcctacaac gatagctaat
    15721 gatttatatt gtaaactcat atgaccttgt tgaatacctt ctgacacaag cgcgcgacat
    15781 gctgcaaagt tttgcgctaa accaacggca gcaactacat gacctaattc ttgtgctgac
    15841 tctacattta gtagctctaa tgaagcttta gctattggta atactttcgt accaccccca
    15901 acaattgcta atgtcattgg cacttcaatt gtaccaatca atcgttgacg atcttgatcg
    15961 taacgccatg tagcaatacc acgatactgt ccgtcacgac tcgcatatgc atgcgcactt
    16021 gcttctgcac cacgcgtatc atttcctgtt gctaaaacaa cggcatgtat gccattcata
    16081 acacctttat tatgtgttgc agcacgatga atatcaactt gtgccaatac agaagcacgt
    16141 tccattcgtt tggcaacctc ttccccagtt ctctcgcccc ttgctaaatc tttaacatca
    16201 atttcgcctt gaactttaac aacggacgct gttgcatgat tggataaaat actcattaaa
    16261 atatcgcttt gcggaaattc attttttaaa aatgcagtta tggcctctaa aatcgtatta
    16321 agcatattag cgcccatagc atctttcgta tcaacaaata cttttaaaga tagtaactgt
    16381 tgctcaggaa atgtatcaat cgctatacgt tggtaaccac caccacgcgc tttaatagaa
    16441 ggatatgcct catccgcaat tttatgaatt tgcttttcta aagctttaat gtctgctgat
    16501 aatttttcag tatcgtcaac gccatcaaag acgatttgac ctatcataat acgttcagaa
    16561 gataccgttt taaatccgcc agtctgattc actagctttg caccataact agctgcagcg
    16621 acaactgaag gctcttccac catcataggt acaacatatg ccttatcgtc cacaatgata
    16681 ttcggtaata atccaacggg taatgcacct tgcgcgatga cattttcaat taaactattg
    16741 gctacttctt catcgattaa tggatgattc agtaaaatgt cgaattgttc ttctgataac
    16801 cattgcttat caaccaattg ttgtaacttt tctttacgag ataaatgtcg aaaattctta
    16861 tctaaatttt gcatggacgt actcctttta cttcacataa tttttaacat ttcaatcact
    16921 actattttta ccacaaaata acgtcattcg tctta
//
LOCUS       DAHHYJ010000050        16725 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000050 DAHHYJ010000000
VERSION     DAHHYJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.050"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            434..1372
                     /locus_tag="NBK22_001221"
     CDS             434..1372
                     /locus_tag="NBK22_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD2414415.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKRAEQVVENIKAADIVLSDDEIQYIDELFPIED"
     gene            1871..2407
                     /locus_tag="NBK22_001222"
     CDS             1871..2407
                     /locus_tag="NBK22_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCD2414416.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            2576..3052
                     /locus_tag="NBK22_001223"
     CDS             2576..3052
                     /locus_tag="NBK22_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2414417.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYQPEMYGEKLDLALMYKAF"
     gene            3092..4387
                     /locus_tag="NBK22_001224"
     CDS             3092..4387
                     /locus_tag="NBK22_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD2414418.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVSPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            4415..4936
                     /locus_tag="NBK22_001225"
     CDS             4415..4936
                     /locus_tag="NBK22_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCD2414419.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            5435..6445
                     /gene="adhP"
                     /locus_tag="NBK22_001226"
     CDS             5435..6445
                     /gene="adhP"
                     /locus_tag="NBK22_001226"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCD2414420.1"
                     /translation="MRAAVVTKDHKVSIEDKKLRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            6520..6628
                     /locus_tag="NBK22_001227"
                     /pseudo
     CDS             6520..6628
                     /locus_tag="NBK22_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6721..7137
                     /locus_tag="NBK22_001228"
     CDS             6721..7137
                     /locus_tag="NBK22_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCD2414421.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            7134..8795
                     /locus_tag="NBK22_001229"
     CDS             7134..8795
                     /locus_tag="NBK22_001229"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCD2414422.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGAKWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVNAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLAAHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            9067..9702
                     /locus_tag="NBK22_001230"
     CDS             9067..9702
                     /locus_tag="NBK22_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCD2414423.1"
                     /translation="MLGTDELYKVLYEHLGPQFWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQVKEHTHFNPNHILELPIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGGLRKELLSLKGIGSETADVLLVYIFGRIEFIPDSYTRKIYDKLGYENTKN
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            9958..10845
                     /locus_tag="NBK22_001231"
     CDS             9958..10845
                     /locus_tag="NBK22_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2414424.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNDKQGSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKEGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            10994..11944
                     /locus_tag="NBK22_001232"
     CDS             10994..11944
                     /locus_tag="NBK22_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD2414425.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            11999..12718
                     /locus_tag="NBK22_001233"
     CDS             11999..12718
                     /locus_tag="NBK22_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD2414426.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            12702..13502
                     /locus_tag="NBK22_001234"
     CDS             12702..13502
                     /locus_tag="NBK22_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2414427.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            13638..14144
                     /locus_tag="NBK22_001235"
     CDS             13638..14144
                     /locus_tag="NBK22_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414428.1"
                     /translation="MKKLLTASIIACSVLMGVGIANTNAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
     gene            14306..15022
                     /locus_tag="NBK22_001236"
     CDS             14306..15022
                     /locus_tag="NBK22_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414429.1"
                     /translation="MRINDKILLENIEDYFNHKGLSPHLIDDIKEKVITDIKNSEKKD
                     QDYIEYKRKSPAQIILMIQRNLFALQMNPVIFFIINFILISYLYDKQYVQFQAITGMS
                     LFYCLVIFPMTIVVYLRVSQKNYLRSNKIEMIMGTIIAIISLLLIILQAFNITWGVIP
                     ITNFGHQFFFFIGIILVIAGIFYKRLEFSGIGLLFCQKTVDAMIHNPQSAQIFSLIIW
                     ILLVVLVIYFTIRLSSRTRL"
     gene            15191..15979
                     /locus_tag="NBK22_001237"
     CDS             15191..15979
                     /locus_tag="NBK22_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2414430.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYKVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSIKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLNFLKSVT"
     gene            complement(16231..16605)
                     /locus_tag="NBK22_001238"
     CDS             complement(16231..16605)
                     /locus_tag="NBK22_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCD2414431.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT         6079 a         2164 c         3089 g         5393 t
ORIGIN      
        1 aaatctaaaa ttattaaatt taatataaaa atttgactca tagatagaat gaataaaaat
       61 ccgaaatata gaattatcaa ctacaaagat gaatatttta taatggatat cccatgttaa
      121 tattaacagt tttaatttct ttgttatgca aatcaaaaca ttatagtatt tctaattgaa
      181 tttgtaatgg ctttaatgtt gtcgttctta aatgtttttc aatttctgat tcaaaagtac
      241 atgtaattta aaggtcaacg agcaatcgcg ctatatgcat atcaatttaa gcaatactta
      301 ttttaactga gttttatata acgttttcct attacaccgc ttaaattcaa gctctaattc
      361 taacatttgg aagttgtatt aaatcatgat agtgtgatag tggaaaagtt ataaagataa
      421 ggagctgggt ctaatgaagc aatttgtaaa cttaggtaaa tctgatgttg aagtgtttcc
      481 aatcgcactt gggacgaacg cagtaggtgg gcataattta tatccgaact tagatgaaga
      541 acaaggaaaa gatgttgttc gtcaagccat taatcatggt attaatttat tagatacggc
      601 atatatttat gggccagaac gatcagaaga attggttgga gaagttgtta aagaatatcc
      661 gcgagagcaa attaaaattg ctacgaaagg gtctcatgaa tttgatgaaa atcaagaagt
      721 acatcagaac aatcaaccgg aatatttaaa acaacaagtt gagaatagtt tgaaacgtct
      781 acaaactgat tatatcgatt tatattatat tcattttccg gatgacaaca ctccgaaaga
      841 tcaagcagtt gcagcattac aagagcttaa ggaacaaggg aagattaaag ccattggtgt
      901 atcaaatttc acattagatc aacttaaaga agcaaataaa gatggttacg ttgatgttgt
      961 acagttagaa tataatttat tgcatcgcga aaatgaggca gtattacaat attgtgttga
     1021 tcaccaaatc acatttattc catatttccc attagcatcc ggtattttag ctggaaaata
     1081 tgatgagaac actaaattta gtgaccatcg tactacacgt cgtgatttta aaccaggtgt
     1141 atttgaagaa aatgtgcgtc gcgtaaaagc tttggaaagc atagctgcag cacatcaaac
     1201 ttcaattgcg aacattgtat tagcatttta tttaacgaga ccagctatcg atgtgattat
     1261 tcctggtgca aaacgtgcag aacaagtcgt tgaaaatatt aaagctgcag atatcgtttt
     1321 atcagatgat gagattcaat atatcgatga actgtttccg attgaagact aaatgaattg
     1381 tatttaatgg tgatatcagg ctgggacata aatcaatgtt ctatgctcta cgaagttata
     1441 ttggcagtag ttgactgaac gaaaatgcgc ttgtaacaag cttttttcaa tcctagtcag
     1501 gggccccaac acagagaatt tcgaaaagaa attctacagg caatgcaagt tggggtgtgg
     1561 gccccaacac agagaatttc aaaaagaaat tctacaggca atgtaagttg gcggggcccc
     1621 aacatagaag ctggcgaaaa gacagcttac aataatgtgc aagttggggg gacgacgaaa
     1681 taaattttgc gaaaatatca tttctgtccc actcccatca caaacgacgt tatataaggc
     1741 atgacgaaat ttcgtcaaat ttatataaaa gttgtaagcg ttttactatt cggtaggcaa
     1801 agcttatgat agtataatta ggtaacgaaa aacacagata tttattaatc aaaagtgagg
     1861 ttaggctaaa atgattactg ttttgtttgg tgggagtaga ccaaacggta atactgcaca
     1921 attaacaaaa ttcgctttgc aagatttaga gtatcaatgg attgacgtga cacaacatca
     1981 gtttaaaccg atacgtgacg tgagacatac agcagagact attacttcat atgacgatga
     2041 ctatttgccg attctagata aaatattggc tagtgataca attatttttg catcaccagt
     2101 gtattggtat agcatttcag caccattgaa agcatttatc gaacattggt cagaaacatt
     2161 acaagataaa cgatatccta attttaaggc acaaatggcc gaaaaggatt ttagagttat
     2221 tttagttggt ggagattgtc caaaaataaa agcgaagcca gcaattacgc aaatgaaata
     2281 tagtttagac tttttaggtg ccactttaaa tggttatatt attggaactg ctgaaaagcc
     2341 tggtgacatc atgaaagaca actatgcctt agcacgtgca actgagtgga atagtatatt
     2401 gcaataagac atacctcata ctaaacgaag tgattttttg cttcgttatt ttttattttt
     2461 aattttaacg gaaaatataa atatataaat tgaatagaca atcattgttt attaagtatt
     2521 gagcaagttg tggtataatg ccgttattaa aacttgttaa taaggggaga aagtcatgtt
     2581 caaggtaaga caagcaactg aaaaagatgt tgttcaaatt agagatgtcg caactaaagc
     2641 ttggtttaat acatacttaa atatatacgc tgcgacaaca gttaatcact tgttagaagc
     2701 ttcatataat gaacatcatt taaagaaaag acttcaagaa caattattct tagtcgttga
     2761 agaaggtaat gacatcgttg gctttgctaa ctttatttac ggtgaagaat tatatttatc
     2821 agctcattat gttaaaccag aatcgcaaca tacaggttat ggtacagcat tgttaaatga
     2881 aggattatca cgttttgaag ataaatttga aggtgtttac ttagaagtag ataataaaaa
     2941 tgaagaagca gtagcttact ataaagagca aggttttaca atcttacgct cttatcagcc
     3001 agaaatgtat ggcgaaaagt tagacttagc acttatgtac aaagcatttt aagtaaatta
     3061 aatattatat taaacgaaaa ggaggggaat aatgactaac gcatatgtag atttaaaatt
     3121 agtagaagaa aaagttttta aagacccgat acatcgatat attcatgttg aagatcaatt
     3181 gatatgggat ttaattaaaa ctaaggaatt ccaaaggtta cgtcgaatta gacaactagg
     3241 aacactgtac ctatcttttc acacagcaga acatagtcgc tttggacatt ctttaggtgt
     3301 gtatgaaata gttagacgat taattgatga gtcatttatt ggtcatgatg catgggacaa
     3361 taaagataga ccgttggcat tatgtgctgc attattacat gatttaggac atggtccatt
     3421 ttcacatagt tttgaaaaaa tatttaatac agaccatgaa gcatacacac aagcgattat
     3481 tactggagat actgaggtga atgctgtatt acgtaaagtg tcgcctgagt ttccaagaga
     3541 agttgcggaa gtaattaata aaacgcatca taataaattg gtcatttcga tgatttcgtc
     3601 acaaatcgat gcggatagaa tggattattt acaacgtgat gcgtatttca caggtgtatc
     3661 atatggtgct tttgatatgg aacgtatttt aagattaatg cgaccttcta aagatgaagt
     3721 actaattaaa gaaagtggta tgcatgcagt tgaaaacttt attatgagtc gttatcaaat
     3781 gtattggcaa atttatttcc acccagttag tcgtggtgga gaagtgctgc ttaataattg
     3841 tttgaaacgc gcaaaacagc tttataatga aggctatgaa tttaagttgc atccacatga
     3901 ttttattcca ttttttgaag agacagttac gattgaacaa tatgttgaac tcgatgaagc
     3961 ggtagttacg tattatttgg aaaaatggac aaaagaagat gatgctattt taagtgattt
     4021 agcaagtcga tttattaatc gagacttatt taaatatatt ccatttgatg gctcaattat
     4081 tacaatatca gaactgcaag aactgtttga agcaggtggt attaatccag attattattt
     4141 tgtgagtgaa gcattttcgg atttgccata tgactatgat cgacctgggt caaatcgcaa
     4201 accgattcat ttattaagac aagatggtac gattagagaa ataagcaatc aatctttagt
     4261 cattcatagt attacaggca ttaatcgcca agactataaa ttatattatc ctagagaaat
     4321 ggttgcaaag attaaagata agacaattag agaagctatt gaaaatttga ttaatgagct
     4381 taattaaaca gggctaaaat tgttatcgtt aaatatggag gttatatcat tgtctgagaa
     4441 aaaaggcttt aattttaata tcataaaaaa tgaccctcta gatggtcata aaggtacaaa
     4501 tattggttca attagcttag acaatattgc accagttttt atcgatgttg ctaacaaaga
     4561 agcatttatt gacattggag gcatgcatgc tcgcgccaaa gttgaaaaag gcgtgaaatg
     4621 gattactgat aaagctgctg ttgaaggcga tgaagctaaa gaatattggt tgtgttgggt
     4681 aacaacagaa cgtaatgaac aaggaccata ttatgctggt ttaacagcgt gctatttatt
     4741 agtgaataaa gcaattcgtc gtggttataa aagtatgcct gaacatgtta atatgatgga
     4801 taaatcaatg aaacatcata ttatcattga tcaaattggt gacgagaata aagctatttt
     4861 aaaagacttt ttaatgaacc atgatgaagg tatgtggaag cattcttctg atgctttaca
     4921 tcaagcattt aattaaatat tagaaactaa aatttcccaa ttaatctata aagatatgat
     4981 tcatttctca atgacgacgt aaatcttgag gtgaaaataa agtcttagat ttattgggag
     5041 tttttttaat ttttttgaaa ttaaattaat ctgtagttaa taaaaaattt gaataactga
     5101 cacatttttt tgatcatagc tatatacttt gtgaattaat tcacattata ataagagtga
     5161 agataagagt attataaatt atctttaaat aaatatatgt gaagtaaaaa ttacacgtta
     5221 gcatatcgat tatgtcattt cttttaacat attaactagg gaaacgttaa aagttaacgg
     5281 ttgatatcta actaaaaaca aagtcacagt agtatgtttt aatctggcgt ctattacaaa
     5341 taaaaattac atctataatt attcgttttc atttttgaaa gtaatcgcca attaatatca
     5401 tacatactgg agtgactata aggaggacat tattatgaga gcagcagttg taacgaaaga
     5461 tcacaaagta agtattgagg acaaaaaatt aagagcttta aaacctggtg aagcgttggt
     5521 acaaacggaa tattgtggcg tttgtcatac cgatttacat gttaagaatg ctgattttgg
     5581 tgatgttaca ggcgttactt taggtcatga aggtattggt aaagtcatcg aagttgcgga
     5641 agatgtagaa tcattaaaaa ttggagaccg tgtgtctatc gcttggatgt tcgaaagctg
     5701 tggaagatgt gaatattgta caacaggtcg tgaaacactt tgccgtagtg tgaaaaatgc
     5761 tggttataca gtagatggtg caatggctga acaagttatt gttactgcag actatgctgt
     5821 gaaagtacct gaaaaattag atccagcagc agcgtcttct attacatgcg caggtgtgac
     5881 aacttataaa gctgtaaaag taagtaatgt aaaacctgga caatggttag gtgtttttgg
     5941 tataggtggt ttaggtaacc tagctttaca atatgctaaa aacgttatgg gggctaaaat
     6001 tgttgccttc gatattaatg atgataaatt agcattcgcg aaagaattag gtgcagatgc
     6061 tattattaat tctaaagatg ttgatccagt tgcagaagtt atgaaattaa ctgataacaa
     6121 aggattagat gcaacagtgg taacttcagt tgctaagacg ccatttaacc aagcggttga
     6181 tgttgtaaaa gctggtgcaa gagttgttgc cgttggttta cctgttgata aaatgaactt
     6241 agatatccca agattagtgc ttgacggtat tgaagtagta ggttcacttg ttggtacaag
     6301 acaagactta cgtgaagcgt ttgaatttgc tgctgaaaat aaagtaacac ctaaagttca
     6361 attaagaaaa ttagaagaaa ttaatgatat ttttgaagaa atggaaaatg gtactataac
     6421 tggtagaatg gttattaaat tttaaaaata tcaactgact atatagataa agaaggtagt
     6481 gctctgaaca ctatcattat aatcaaacac gaggttttca tgaaagatag tgaaaatctc
     6541 gtgttttttt ggttttgagg tgttgtttgt attttataaa atggcttaca tatatgaagc
     6601 gttgattaag tatggaattg ttaattaatt gaacctattt agctttaaga aggcataaca
     6661 agatgacctt attttatgct ataatatttc tattatgcga agattaaggt gagtagtaaa
     6721 ttggataaaa aagtaagtat tcaaacaaag caagtgttga aacagcacaa cgaaaaagaa
     6781 aaatttgaat ttactactga aggaacttgg caacaaaggc aatctaactt tattcggtat
     6841 gtagaacaaa ttgaggatgc aacagttaat gttacaataa aagtggatga tgatagcgtt
     6901 aagttgattc gtaaaggcga cattaatatg aatttgcatt ttgttgaagg acaaacgaca
     6961 acaacttttt acgatatatc ggctggacga attccactag aagttaaaac attacgcatt
     7021 ttacatttcg taagtggaga cggtggcaag ctaaagattc attatgaatt atatcaagat
     7081 aatgaaaaaa tgggttctta tcaatatgaa attaactata aggagatagg cgaatgaata
     7141 ttattgatca agtgaaacaa acattagtag aagaaattgc agcaagtatt aacaaagcag
     7201 gattagcaga tgagattcct gatattaaaa ttgaagttcc taaagataca aaaaatggag
     7261 attatgctac taatattgcg atggtactga ctaagattgc aaagcgtaat cctcgtgaaa
     7321 ttgctcaagc gattgttgat aacttagata ctgaaaaagc acatgtaaaa caaattgaca
     7381 ttgctggtcc aggattcatt aatttttact tagataatca gtatttaaca gcaattattc
     7441 ctgaagcaat tgaaaaaggt gatcaatttg gacatgtaaa tgaatcaaaa ggtcaaaatg
     7501 tattgcttga gtatgtttca gctaacccta caggagattt acatattggt catgctagaa
     7561 atgcagcagt tggtgatgct ttagctaata ttttaactgc agctggctat aatgtaacac
     7621 gtgaatatta tattaatgat gctggtaatc aaattactaa cttagcgcgt tcgattgaaa
     7681 cacgtttctt tgaagcttta ggtgacaata gttattcaat gccagaagat ggctataatg
     7741 gaaaagatat tattgaaata ggaaaagatt tagcagagaa acaccctgaa attaaagatt
     7801 attctgaaga agcacgtttg aaagaattta gaaaattagg cgtagaatac gaaatggcta
     7861 aactgaaaaa tgatttagca gagttcaata cgcattttga taattggttt agtgaaacat
     7921 ctttatatga aaaaggcgaa attcttgaag ttttagcaaa aatgaaagaa ttaggttata
     7981 cgtatgaagc tgatggcgct aaatggttac gtacaactga ttttaaagac gacaaagaca
     8041 gagtattaat taaaaatgac ggtacatata cgtatttctt accagatatt gcgtaccact
     8101 tcgataaagt taaacgtggt aatgacattt taatcgattt atttggtgct gatcatcatg
     8161 gttatattaa tcgtttgaaa gcatctcttg aaacgtttgg tgtagatagt aatcgtttag
     8221 aaattcaaat catgcaaatg gttcgtttaa tggaaaatgg taaagaagtg aagatgagca
     8281 aacgtactgg taatgccatt acattaagag aaattatgga cgaagttggc gttaacgctg
     8341 cacgttattt cttaactatg cgtagtcctg atagtcactt tgattttgat atggaattag
     8401 cgaaagagca atctcaagac aatccagttt actatgctca atatgcacat gcgcgtattt
     8461 gttcaatttt aaaacaagca aaagagcaag gtattgaagt gactgctgcg aatgatttta
     8521 caacaattac taatgaaaaa gcgattgaat tgttgaaaaa agtagctgac tttgagccta
     8581 caattgaaag tgctgcagaa catagatcag cacatagaat tactaattat attcaagatt
     8641 tagctgctca tttccataaa ttctacaatg ctgaaaaagt gttaacagat gatattgaaa
     8701 aaacaaaagc acatgttgct atgattgaag cggttagaat tacattgaaa aatgctttgg
     8761 caatggtcgg tgtaagcgca cctgaatcaa tgtaagaaca tttatataca ctccaacgta
     8821 gagtttctcg aaagatactt tgtgttggag tgtttttaat taggtatgtg acatattggg
     8881 gaatgcttag tatgtgaata aggttaagag gaacgcagtt ggatgctact gcacaactgc
     8941 ataagagagc ctaaatcgct aaagctcaag gggagtaaag gaaaatacag ttggcttgct
     9001 tgcgcagcaa ctgcataaga gcccctaatt aatttattaa ttaggggctc taaaaggagg
     9061 atgtaaatgt taggaactga tgaattatat aaagttttat atgaacatct cggaccacaa
     9121 ttttggtggc ctgctgataa tgacattgaa atgatgttag gtgcaatttt agttcaaaat
     9181 actagatggc gaaatgcaga aattgcattg aatcaagtta aagaacacac gcattttaat
     9241 ccaaatcata tattagaact acctattgaa acgttacaat cattgataca ttcaagtggc
     9301 ttttataaaa gtaaatcact gacgattaaa acattattaa catggttagc acgacatcat
     9361 ttcaattatc aagagattaa tgagcgatat aaaggtggat taagaaaaga attattatct
     9421 ttgaaaggta ttggaagtga aacagcagat gtcttacttg tttatatatt cggacgtatt
     9481 gaatttattc cagatagcta tacaagaaaa atatatgata aattaggata tgaaaacact
     9541 aaaaattatg atcaattaaa aaaagtagtc acattaccaa atcattttac aaatcaagat
     9601 gctaatgaat ttcatgctct gttagatgta tttggtaaac attactttag agacaaagat
     9661 ataaagaatt atgatttttt agaaccttac tttaaaaagt aaacgctgtg aagttagata
     9721 gatgagttta tatgaaatat aaaaaataat ttactatttt cttttagtat gtggacttat
     9781 ataataaata gaagcatata aagaaaaaaa cagttgtttg tttgtgcagc aactgcataa
     9841 gagcccctaa tcgctaaagc tcaaggggag taaaggaata cagttgtttg tgcagcaact
     9901 gcataaaagc ctctaatcgc taaagctcaa gaggctttaa aaaggagatg tgataatgtg
     9961 aagaaatcgt taattgcttt tattttgatt tttatgcttg tcctgagtgg ctgtggtatg
    10021 aaagataatg ataaacaagg tagcgatgat aatggctcgt ctaaatcgcc gtaccataga
    10081 attgtttcgt taatgcctag taatactgaa attttatatg aattaggatt aggtaaatac
    10141 atagttggtg tttcaacggt tgatgattat ccaaaagatg tgaaagaggg taagaaacaa
    10201 tttgatgctt tgaatctaaa taaagaggaa cttttaaagg caaagccaga tctaattctt
    10261 gcgcatgagt cgcaaaaggc aactgctaat aaagtattgt catcattaga gaaacaaggc
    10321 atcaaagtag tgtatgttaa agatgcacaa tcaattgatg aaacatacaa cacatttaag
    10381 caaattggga aattaacgca tcatgataag caggctgaac aacttgttga ggaaactaaa
    10441 gataatatcg ataaagtcat agattcaatt cctgctcatc ataaaaaatc aaaagtattt
    10501 attgaggttt catcaaagcc tgaaatatat acagcaggga agcatacatt ttttaatgat
    10561 atgttagaaa aattagaagc ccaaaatgtt tatagtgaca ttaatggttg gaaccctgta
    10621 acgaaggaaa gtattattaa aaagaaccca gatatattaa tttcgacgga agctaagaca
    10681 agatcagatt atatggatat catcaaaaaa aggggtggat tcaataaaat taatgctgtc
    10741 aagaatacac gtattgaagt tgtaaatggt gatgaagtat caagaccagg tccacgtatt
    10801 gatgaaggat taaaagaatt aagagatgca atttatagaa aataaaccat tctaattatg
    10861 ccccttattg ctacatgtaa aaaaatacat gtttgagata aggggttttt aaaatatatt
    10921 tagtgaatga tagcaacgcg agtatgtgat tgctataatg aatgtaatta tcgatgaaag
    10981 aaaagagaat gctatgacat ttaataaagt attattgagc tggatagtca tattgattat
    11041 aacaactagc atatatctat tttggcagtt gggggatatc aatgatgtat ttaaccagtc
    11101 tattttaatc aatgttagat taccgagatt attagaagca ttgttgacag gtatgatatt
    11161 aacagttgca ggtcttatat ttcaaacagt tttaaataat gcattggcag atagctttac
    11221 attaggattg gcaagcggtg ctacatttgg ttcaggatta gcattatttt taggtttaac
    11281 aacgttatgg attcctgtat tttcaataac atttagtttg ataacattaa taactgtatt
    11341 agtcattacg tcggtattga gccaaggcta tccagttaga atcttaatat taagtggttt
    11401 aatgattggt gcattattca attcacttct atattttttg attttattaa aacctcgcaa
    11461 attaaataca attgccaatt atttgtttgg tggctttggt gatgcagaat actcaaatgt
    11521 atccataata gcaatcacat ttatcattgc actgtttggt atatttatca ttcttaatca
    11581 actaaagtta ttgcaattag gagaactaaa aagtcagtca ctaggcttaa atgttcaatt
    11641 gattacatat atcgcgttat gtatagcttc tatgataacg gcgataaatg tcgcatatgt
    11701 tggcatcatt ggattcattg gtatggtgat accacaactc attagaaaat ggcagtggaa
    11761 acaatcatta ggaagacaat tggctttgaa tattgtaatt ggaggacaaa taatggttat
    11821 ggcagatttt attggtagcc atatactgtc accagtacaa ataccggcaa gcattattat
    11881 tgcattaatt ggtataccag tgttatttta catgctaata tctcagtcga aacggttaca
    11941 ctagcacatg acatttgcta aaataaaaat aactataaac ataaagaggg cataagcgat
    12001 ggatttgaat caaattaaag cagttgtatt tgatttagaa ggtacgttgt tggacagagt
    12061 taaatctcga gagaaattta tcgaagagca atatgaacga tttcatgact acttaattca
    12121 tgttcaactg gcagatttta aaaaagcatt tattgagcta gatgacgatg aagataatga
    12181 taaacctgat ttatataaag aaatcattaa acgtttccat gtagataggt taacttggaa
    12241 agacttattt aatgattttg aaatgcattt ttatcgttat gtatttcctt attacgatac
    12301 tttgtataca ctagaaaagc tatcgcaaaa aggctttcaa attggtgtta tcgcaaatgg
    12361 taaatctaag attaaacaat ttcgattaca ttcacttggt ttgatgcatg ttattaatta
    12421 tttatcaaca tcagaaacag ttggttttcg taaaccacat cctaaaattt ttgaagatat
    12481 gattgatcaa ctaggggtat tacctgagca aattatgtat gttggcgatg atgcgttaaa
    12541 tgatgtagct ccagcacgag ctatgggcat ggttagtgta tggtataaac aagaagatgc
    12601 tgaaattgaa ccactcgaag aagaagttga ttttacaatt acaacagtgg aagaattatt
    12661 aaccatttta ccaataaaaa atgataataa aggagaaaat tatggatcta tttactagaa
    12721 aagatggaac atcgatacat tacagtacat taggtgaagg ctatcctatc gtattgattc
    12781 atactgtact tgataattat tctgtgttta ataaattagc agcagaatta gcaaaatcat
    12841 ttcaagttgt gttaattgat ttacgtggac atggctattc tgataaacct cgtcacattg
    12901 aaataaaaga tttttctgat gacattgttg aattacttaa atatttatat attgaagaag
    12961 ttgcatttgt atgccatgaa atgggtggaa tcattggtgc ggatatttca gtacgttatc
    13021 ctgaatttac atcatcactt acgttggtaa atccaacatc tattgaaggt gaattaccgg
    13081 aagaacgttt atttagaaaa tatgcccata ttattcgaaa ctgggatcct gaaaaacaag
    13141 ataaattttt aaataagcgt aagtattatc gtccgagaaa aatgaatcga ttcctcaaac
    13201 atgtcgtaga tacaaatgaa atatcaacta aagaagaaat tcaagcagtt aaagaggtat
    13261 tcaaaaacgc tgatatttct caaacttata gaaatgtcgt agtaccgaca aaaattattg
    13321 caggagaatt cggtgaaaga acaacaagat tagaagctaa agaagtagct gatttaatcc
    13381 aaaatgcgga ctttgaagta tatcaagaat caagtgcatt cccatttgtt gaagagcaag
    13441 aaagattcgt cgaagataca gcttcattta tcaacaaaca tcacgatgaa aagcatgttt
    13501 aatttttagt gataagtgaa atataaaagt agtaattatt atttgtaata taattgtaat
    13561 atgactgttg ttttagaaat gattgttgta taatggttgt gagcacaaga cgatataaat
    13621 aaaaggagag agtgcttatg aaaaagctac taacggcaag tataattgca tgttctgttt
    13681 taatgggagt aggcatagcg aacactaatg ccgaagcagc aagtggcaac tctattgata
    13741 ctgttaaaca attaattaag ggtgatcagt cattagaaaa tgtgaaaatt ggcgaatcta
    13801 ttaaagatgt tttaactaag tacaaaaatc ctatgtattc ttacaatgaa gatggaactg
    13861 aacattatta cgaattccat actaaaaaag gtatgttatt agtaactact gatggtaaga
    13921 aaaacaatgg taaagtaact catatttcaa tgatgtataa tgatgctaat ggtccaacat
    13981 atcaagctgt taaaaattat gttggcaaag cagtaacaca tacggaatat agcaaagttg
    14041 ctggtaattt tggatatatt gaaaaaggca aaacgactta tcaatttgcc tcagcaccaa
    14101 aagataaaaa cataaaatta tatcgtattg atttggaaaa ataataatta aattaatcgt
    14161 gcttaaccga caagttaaat cgcaatactt gtcggttatt ttattacatg gcataataca
    14221 gtgaatatta atttttatga tttgcgaagg ggaaattaaa ccaatacaat aaataaagat
    14281 gtttcatttt aaaggagtac taccaatgcg tattaacgat aaaattttac tagaaaatat
    14341 agaagattac tttaatcata aaggtttatc accgcatttg attgatgata ttaaagagaa
    14401 agtaattact gatataaaaa attctgaaaa gaaagatcaa gattatattg aatataaaag
    14461 aaaatctcca gcacaaatca tattaatgat tcaaaggaat ttgtttgctt tacaaatgaa
    14521 tccagttatt ttctttatta taaacttcat tctcatatcc tatttatatg ataaacagta
    14581 tgttcagttt caagctatta ctggaatgag tctattttat tgtttagtga tttttccaat
    14641 gactattgtt gtatacctaa gggtgtctca aaagaattac ttgcgtagta ataaaataga
    14701 aatgattatg ggtacaatta tcgctatcat atccttgtta ttaattatat tacaagcatt
    14761 taatattact tggggcgtta taccaattac aaattttgga catcaatttt tctttttcat
    14821 tggtattatt ttagtaattg ccggcatatt ttataagcga cttgagtttt cgggaatcgg
    14881 gttattattt tgtcaaaaaa ccgtcgatgc aatgattcat aatccacaat cagcccagat
    14941 tttttcatta attatatgga tattattagt agttctagtt atatatttca caattagatt
    15001 atcttcacgt acaagattat aaatatgata aaactattca cttgattaat tgtattaatt
    15061 gagatgaata gtttttttat tgttggaata acttttggta atttataaat aatttaaaaa
    15121 aattgtttat aaaatggaag cgtatataga atgaaggttg ggtatatagt ttattgaggg
    15181 aggtgtcaca atgaataaag tcacaattaa tcctcaaatc caattaactt atcaaattga
    15241 aggtaaaggg gatcctataa tattacttca tggattggat ggtaatttag ctggatttga
    15301 agatttgcaa catcaactag catcatcata taaagtactt acttacgatt taagaggtca
    15361 tggcaagtct tctaaaagtg aatcatacga tttaaacgat cacgttgagg atttaaaaat
    15421 tctaatggag aagttaaata ttcatgaggc acatattcta ggacatgatt taggtggggt
    15481 agttgctaag ttatttacag ataaatatgc ttatcgtgta aaatcattaa ctaccattgc
    15541 atcgaagaaa gatgacttaa tacacagctt tactcaattg ttaatacaat atcaagatga
    15601 tatagcgggt tttaataagt ctgaagcgta tattctttta ttttctaaat tgtttagaaa
    15661 tcaagagaag acgatgaaat ggtatcaaaa acaaagaata tatagcatta agtctgagga
    15721 tgatagtgcg gtggcaattc gttcattaat tttgcataaa gatgaaccta tgtatttaaa
    15781 aaaacgtaca tgtgtaccta ctttgttaat taatggggaa catgatcctt tgattaaaga
    15841 taagaatcat tttaaattgg aagcgcattt tttaaatgtt acgaaaaaaa tcttcgaaca
    15901 ttcaggacat gcaccgcata ttgaagaacc agaagcattt atgaattatt atttaaattt
    15961 tttaaaaagc gtaacataat atgtgatata taaacctagg gcataaagtc cttaggcaat
    16021 gtgaaaaagc tgattactat tcattatttg atagaaatca gctttttttg aaatgtattt
    16081 gatatatact gcttgttatg cggctatctt ccttatatta agtgccatta gtgcaaaacc
    16141 tcttaacaat taggtaaaaa gagcataaaa aaaaggaagt ttaatagaat gtatcatcta
    16201 tcaaacttca ccaaattgcg ctaaacaaaa ttatagttca atttcgttgt ttgcttcagt
    16261 gattcgttta tttactcgac tcaataatga ttcgattttt ttacgttgtt gtgcattaac
    16321 aagaattaat acagttcttt catcatgctc attacgtttt ttatcgaagt aatcttcttg
    16381 agataaaatt ttaactgctt taacaacttg tggttgtttg tagtttaaat gattaataat
    16441 atctttaaga tagtattctt tctctttgtt ttcgctgatg tatgtcaata cagcgaattc
    16501 ttcaaagcta attgaaaatt cctttttaat taaacttttt aatttgtcag cataagtgac
    16561 cattgataac aactcaaagc aatcattgat ttttgtaatt gccatgttta aaacctccct
    16621 atttgatgca tcttgctcga tacatttgcc cgataatata ttgatatcta atctttattt
    16681 attatagata tgttagtcat aattttgcat taaataagtt ttatt
//
LOCUS       DAHHYJ010000051        16686 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000051 DAHHYJ010000000
VERSION     DAHHYJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.051"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            20..985
                     /locus_tag="NBK22_001239"
     CDS             20..985
                     /locus_tag="NBK22_001239"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCD2414432.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            1135..1788
                     /locus_tag="NBK22_001240"
     CDS             1135..1788
                     /locus_tag="NBK22_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCD2414433.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            2099..2671
                     /locus_tag="NBK22_001241"
     CDS             2099..2671
                     /locus_tag="NBK22_001241"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCD2414434.1"
                     /translation="MKCIVGLGNIGKRFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            2671..6177
                     /gene="mfd"
                     /locus_tag="NBK22_001242"
     CDS             2671..6177
                     /gene="mfd"
                     /locus_tag="NBK22_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCD2414435.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNIEEV
                     DITTASDYIITEEVIRHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIDYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSIKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLKFLVKCIEESMRISDEA"
     gene            6167..7693
                     /locus_tag="NBK22_001243"
     CDS             6167..7693
                     /locus_tag="NBK22_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD2414436.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILIFIAIFVFANN
                     IAHMMGDSHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRFTGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            7693..8886
                     /locus_tag="NBK22_001244"
     CDS             7693..8886
                     /locus_tag="NBK22_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD2414437.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGTDNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            8883..9146
                     /locus_tag="NBK22_001245"
     CDS             8883..9146
                     /locus_tag="NBK22_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCD2414438.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIIEERRLEEA"
     gene            9164..9556
                     /locus_tag="NBK22_001246"
     CDS             9164..9556
                     /locus_tag="NBK22_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCD2414439.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            9661..10062
                     /locus_tag="NBK22_001247"
     CDS             9661..10062
                     /locus_tag="NBK22_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCD2414440.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            10242..11537
                     /gene="tilS"
                     /locus_tag="NBK22_001248"
     CDS             10242..11537
                     /gene="tilS"
                     /locus_tag="NBK22_001248"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCD2414441.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEAYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIKK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDKFKLNGRDGYKKVNRLFIDCKVPQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKKWIIISKNGDE"
     gene            11542..12081
                     /gene="hpt"
                     /locus_tag="NBK22_001249"
     CDS             11542..12081
                     /gene="hpt"
                     /locus_tag="NBK22_001249"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCD2414442.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            12338..14431
                     /gene="ftsH"
                     /locus_tag="NBK22_001250"
     CDS             12338..14431
                     /gene="ftsH"
                     /locus_tag="NBK22_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCD2414443.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
     gene            14660..15541
                     /gene="hslO"
                     /locus_tag="NBK22_001251"
     CDS             14660..15541
                     /gene="hslO"
                     /locus_tag="NBK22_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCD2414444.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            15720..16652
                     /gene="cysK"
                     /locus_tag="NBK22_001252"
     CDS             15720..16652
                     /gene="cysK"
                     /locus_tag="NBK22_001252"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCD2414445.1"
                     /translation="MARKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
BASE COUNT         6007 a         2292 c         3339 g         5048 t
ORIGIN      
        1 aataattgga ggactataaa tgttaaataa tgaatataag aattcgtcat taaagatttt
       61 ttcattgaaa ggaaacgaag cattagcgca agaagttgct gaccaagtag gaattgaact
      121 aggtaaatgt tcagttaaac gttttagtga tggagaaatt caaattaata tcgaagagag
      181 tattcgtggt tgtgacgtat ttattattca accaacatca tatcctgtga atctacattt
      241 aatggaatta ttaattatga ttgatgcttg taaacgtgct tctgcagcaa caatcaatat
      301 tgtagtgcca tattatggat atgcaagaca agatagaaaa gcccgtagcc gtgagccaat
      361 cactgctaaa ttagttgcaa acttaatcga aacagctggc gcaactcgta tgattgcgtt
      421 agacttacat gcaccacaaa ttcaaggatt ctttgatatt ccaattgacc acttaatggg
      481 tgtgccaatt cttgctaaac atttcaaaga tgatccgaat attaacccag aagaatgtgt
      541 cgttgtttca ccagaccatg gcggtgttac acgtgcacgt aaattagctg acattttaaa
      601 aactccaatt gcaattatag ataaacgtcg tcctagacca aatgttgctg aagtgatgaa
      661 cattgttggt gagattgaag gacgtacggc aattattatt gacgatatta ttgatacagc
      721 aggtacaatc actttagctg cacaagcatt aaaagataaa ggtgctaaag aagtatatgc
      781 ttgttgtaca caccctgttt tatcaggacc ggctaaagaa cgtatcgaaa attctgctat
      841 aaaagaatta atcgtaacaa actcaattca tttagatgaa gatcgcaaac catctaacac
      901 taaagaatta tctgttgctg gtttaatcgc acaagctatc attcgtgtat acgaaagaga
      961 atcagttagc gtattatttg actaatattt aaaaggcgtt tgacgaacat attccaaacg
     1021 tgtataatag tttcgttcgt gattatacga ataaataaac acttgcaagc aacgatgatg
     1081 ttgatgggta agtgaggtgc tcgttttgag caaaaatgaa aggtggaaat gagaatggct
     1141 tcattaaagt caatcatccg tcaaggtaaa caaacacgtt cagatcttaa acaattaaga
     1201 aaatctggta aagtaccagc agtagtatac ggttacggta ctaaaaacgt gtcagttaaa
     1261 gttgatgaag tagaattcat caaagttatc cgtgaagtag gtcgtaacgg tgttatcgaa
     1321 ttaggcgttg gttctaaaac tatcaaagtt atggttgcag actaccaatt cgatccactt
     1381 aaaaaccaaa ttactcacat tgacttctta gcaatcaata tgagtgaaga acgtactgtt
     1441 gaagtaccag ttcaattagt tggtgaagca gtaggcgcta aagaaggcgg cgtagttgaa
     1501 caaccattat tcaacttaga agtaactgct actccagaca atattccaga agcaatcgaa
     1561 gtagacatta ctgaattaaa cattaacgac agtttaactg ttgctgatgt taaagtaact
     1621 ggcgacttca aaatcgaaaa cgattcagct gaatcagttg taacagtagt tgctccaact
     1681 gaagaaccaa ctgaagaaga aatcgaagct atggaaggcg aacaacaaac tgaagaacca
     1741 gaagttgttg gcgaaagcaa agaagacgaa gaaaaaactg aagagtaatt ttaatctgtt
     1801 acattaaagt ttttatactt tgtttaacaa gcactgtgct tattttaata taagcatggt
     1861 gctttttgtg ttattataaa gcttaattaa actttattac tttgtactaa agtttaatta
     1921 attttagtga gtaaaagata ttaaactcaa caatgataca tcataaaaat tttaatgtac
     1981 tcgattttaa aatacatact tactaagcta aagaataatg ataattgatg gcaatggcgg
     2041 aaaatagatg ttgtcattat aataataaat gaaacaatta tgttggaggt aaacactcat
     2101 gaaatgtatt gtaggtctag gtaatatagg taaacgtttt gaacttacaa gacataatat
     2161 cggctttgaa gtcgttgatt atattttaga gaaaaataat ttttcattag ataaacaaaa
     2221 gtttaaaggt gcatatacaa ttgaacgaat gaacggagat aaagtgttat ttatcgaacc
     2281 aatgacaatg atgaatttgt caggagaagc agttgcaccg attatggatt attacaatgt
     2341 taatccagaa gatttaattg tcttatatga tgatttagat ttagaacaag gacaagttcg
     2401 cttaagacaa aaaggaagtg cgggcggtca caatggtatg aaatcaatta ttaaaatgct
     2461 tggtacagac caatttaaac gtattcgtat tggtgtggga agaccaacga atggtatgac
     2521 ggtacctgat tatgttttac aacgcttttc aaatgatgaa atggtaacga tggaaaaagt
     2581 tatcgaacac gcagcacgcg caattgaaaa gtttgttgaa acatcacgat ttgaccatgt
     2641 tatgaatgaa tttaatggtg aagtgaaata atgacaatat tgacaacgct tataaaagaa
     2701 gataatcatt ttcaagacct taatcaggta tttggacaag caaacacact agtaactggt
     2761 ctttccccgt cagctaaagt gacgatgatt gctgaaaaat atgcacaaag taatcaacag
     2821 ttattattaa ttaccaacaa tttataccaa gcagataaat tagaaacaga tttacttcaa
     2881 tttatagatg ctgaagaatt gtataagtat cctgtgcaag atattatgac cgaagagttt
     2941 tcaacacaaa gccctcaact gatgagtgaa cgtattagaa ctttaactgc gttagctcaa
     3001 ggtaagaaag ggttatttat cgttccttta aatggtttga aaaagtggtt aactcctgtt
     3061 gaaatgtggc aaaatcacca aatgacattg cgtgttggtg aggatatcga tgtggaccaa
     3121 tttcttaaca aattagttaa tatggggtac aaacgggaat ccgtagtatc gcatattggt
     3181 gaattctcat tgcgaggagg tatcatcgat atctttccgc taattgggga accaatcaga
     3241 attgagctat ttgataccga aattgattct attcgcgatt ttgatgttga aacgcagcgt
     3301 tccaaagata atattgaaga agtcgatatc acaactgcaa gtgattatat cattactgaa
     3361 gaagtgatta gacatcttaa agaagagtta aaaactgcat atgaaaatac aagacccaaa
     3421 atagataaat cagtgcgcaa tgatctgaaa gaaacgtatg aaagctttaa attattcgaa
     3481 agtacatact ttgatcatca aatactacgt cgtttagtag cgtttatgta tgaaacacct
     3541 tcgacaatta ttgattattt ccaaaaagat gcaatcattg cagttgatga atttaatcgt
     3601 attaaagaaa ctgaagaaag tttaacagta gagtctgatt cgtttattag caatattatt
     3661 gaaagtggta atggatttat aggacaaagt tttataaaat atgatgattt tgaaacattg
     3721 attgaaggct atcctgtcac ttatttttca ttattcgcta caacaatgcc gataaaacta
     3781 aatcatatta ttaaattttc atgtaaacct gtccaacaat tttatgggca atatgacatt
     3841 atgcgttctg aatttcaacg atatgttaat caaaactatc atatcgtggt tttggtcgaa
     3901 accgaaacta aagttgaacg tatgcaagcg atgttaagtg aaatgcatat tccatcaata
     3961 acaaaattgc atcgctcaat gtcatcgggg caagcagtga ttattgaggg cagtttatct
     4021 gaaggatttg aactacctga tatgggatta gttgtcatta ctgagcgtga gctttttaaa
     4081 tcaaaacaga aaaagcaacg aaaacgtacg aaagctatct caaatgctga aaaaattaag
     4141 tcttaccaag atttaaatgt gggagattat attgttcatg tgcatcatgg tgttggtaga
     4201 tatttaggtg ttgagacgct cgaagtgggg caaacgcatc gtgattatat taaattgcaa
     4261 tataaaggta cggatcaact atttgttcca gtagatcaaa tggatcaagt tcaaaaatat
     4321 gtagcttcgg aagataagac gccaaaatta aataaactcg gtggcagtga atggaaaaaa
     4381 acaaaagcta aagttcaaca aagtgttgaa gatattgctg aagagttgat tgatttatat
     4441 aaagaaagag aaatggcaga aggttatcaa tatggggaag acacagctga gcaaacaaca
     4501 tttgaattag attttccata tgaacttacg cctgaccaag ctaaatctat cgatgaaatt
     4561 aaagatgaca tgcaaaaatc gcgtccaatg gatcgcttgc tatgtggtga tgttggttat
     4621 ggtaaaactg aagttgcagt gagagcagca ttcaaagctg taatggaagg aaagcaggtt
     4681 gcatttttag ttcctacaac tattttagct cagcaacatt atgagacgtt aattgagcgt
     4741 atgcaagatt ttcctgttga aattcaatta atgagtcgtt ttagaacgcc taaagagata
     4801 aaacaaacta aggaaggact taaaactgga tttgttgaca tagttgttgg tacacacaaa
     4861 ttacttagta aagatataca gtataaagat ttagggctgt tgattgtaga tgaagaacaa
     4921 cgatttggtg tacgccataa agagcgtatt aaaacattaa aacataatgt agatgtacta
     4981 acattgactg caaccccaat acctagaaca ttgcatatga gtatgctagg tgtgcgcgat
     5041 ttgtcagtga ttgaaacgcc gccagaaaat cgtttcccag ttcaaacata tgtattagaa
     5101 cagaacatga gttttatcaa agaagcttta gaaagagaac tatcccgtga tggccaagtg
     5161 ttttatcttt ataataaagt gcaatccatt tatgaaaaac gagaacaact ccagatgtta
     5221 atgccagatg ctaacattgc agttgctcat ggacaaatga cagagcgcga tttagaagaa
     5281 acgatgttaa gttttatcaa taatgaatat gatattttag taacgacgac gattattgaa
     5341 acaggtgtcg atgtcccaaa tgcaaatact ttgatcattg aagatgcaga tcgctttgga
     5401 ttgagtcagt tgtatcaatt aagaggtcgt gttggtcgtt caagtcgtat tggttatgca
     5461 tacttcttac atccagcaaa taaggtacta actgagactg cagaagatcg attacaagcg
     5521 attaaagaat ttacggagtt aggctcagga tttaagattg cgatgcgtga tttgaacatt
     5581 cgtggtgctg gtaatttgtt aggtaaacaa cagcacggct ttattgatac agttggattt
     5641 gatttgtaca gtcaaatgtt agaagaagct gtaaatgaaa aacgtggtat taaggaacca
     5701 gaatctgagg tgccagaagt cgaagttgat ttaaacttgg atgcatattt gccgacagaa
     5761 tatattgcaa atgaacaagc taaaattgaa atttataaaa agctacgaaa aactgaaaca
     5821 tttgatcaaa ttatcgacat taaagatgaa ttaattgatc gttttaatga ttatcctgtt
     5881 gaagtagcac gtttgcttga tatagtggaa ataaaagtac acgcattaca ttcaggtatc
     5941 acgttgatta aagataaagg gaaaataatt gatattcatt tatctataaa agccactgaa
     6001 aatattgatg gcgaagtgct gttcaaagca acacaacctt taggtagaac aatgaaggtt
     6061 ggtgttcaaa ataatgcaat gacaattact ttaacgaaac aaaatcaatg gcttgatagt
     6121 ttgaagtttt tagttaagtg cattgaagaa agtatgagaa tcagtgatga agcataaaga
     6181 agcatttaat ggcgttgtcg tgttaactgc tgcattaatt gtcattaaaa ttctgagtgc
     6241 tgtatatcga attccatatc aaaatatatt aggcgataca ggtttgtatg catatcaaca
     6301 agtgtatcca attgtagcac taggaatgat attatcgatg aatgccattc ctagtgcaat
     6361 tacacaaaat ataggaaagt atcatagtga cgaagcatat gcaaaagcag tcgcttatat
     6421 acaattagtt ggtatattaa tatttattgc tatttttgtg tttgcgaaca atattgcaca
     6481 tatgatgggt gatagccatt taacaccaat gattcaagct gcaagtttaa gctttatatt
     6541 tataggtatg cttggcgtgt taagaggtta ttatcaatct gcaaataata tgacagttcc
     6601 ggctatttcc caggttatag aacaagttat acgagtaggt attatcattg ttactattgt
     6661 tatttttgta gacagaggtt ggacgatata tgaagcggga acaattgcta ttttagcatc
     6721 aacgataggt tttttaggtt cttcaattta tttagtagcg caccgacctt ttaagtttaa
     6781 aatggtaaat aacactgcaa agattgtttg gaaacagttc gcactttcgg ttttgatttt
     6841 cgctatcagt caattaatcg taattttatg gcaagtgatt gatagtgtta ctattattaa
     6901 gtcacttcaa gcgatacgcg tgccattcga tgttgccata actgaaaaag gagtctatga
     6961 ccgtggtgca tcatttattc agatgggatt gattgtaact acaacattta gttttgcgct
     7021 cattcctctg ttaagtgacg caatcaaaat gaataatcag gtacttatga atcgttatgc
     7081 aaatgcgtca ttaaagatta cgattttaat aagtacagca gcgggaatag gattaattaa
     7141 tttattgcct ttaatgaacg gtgtgttttt taagacgaat gatttaacct taacgttaag
     7201 tgtttatatg attacggtca tttgtgtatc tttaattatg atggatatgg cattattaca
     7261 agcgcaacat gctgtgagac ctatttttgt tggtatgacg gcaggattgg ttattaaatt
     7321 tatacttaat atcattttga ttcgtttaag tggcattatt ggtgcgagca ttagtactgt
     7381 tgtatcatta attatattcg gtacgattat ccatattgct gtcacgagaa aataccactt
     7441 acatgcgatg agacgatttt ttatcaatgt tgttttaggt atggtattta tgtcgattgt
     7501 tgttcaatgc gtgttaaaca tagtgacaac acacggtaga ttcactggac tcattgaatt
     7561 attatgtgca gcagtattag gtatcattgc attgtttttc tatattttta gatttaatgt
     7621 tttgacatat aaagagttaa cttatttacc atttggttca aagttgtatc aaattaagaa
     7681 aggaagacgt tgatggcaca taccattacg attgttggct taggaaacta tggcattgat
     7741 gatttgccgc tagggatata taaattttta aagacacaag ataaagttta tgcaagaacg
     7801 ttagatcatc cagttataga atcattgcaa gatgaattaa catttcagag ttttgaccat
     7861 gtttatgaag cacatgacca atttgaagat gtctataatg atattgtggc gcaattggtt
     7921 gaagctgcta atgaaaaaga tattgtctat gcggttccgg gtcatcctag agttgctgag
     7981 acaactacag tgaaattact ggctttagca aaggacaata ctgatataga tgtgaaagtt
     8041 ttaggtggta aaagttttat tgatgatgtg tttgaagcag ttaatgtaga tccaaatgat
     8101 ggcttcacac tgttagatgc gacatcatta caagaagtaa cacttaatgt tagaacgcat
     8161 acattgatta cacaagttta tagtgcaatg gttgctgcta atttgaaaat cactttaatg
     8221 gaacgatatc ctgatgatta ccctgttcaa attgtcactg gtgcacgaag cgatggtacg
     8281 gataacgttg tgacatgccc attatatgaa ttggatcatg atgaaaatgc attcaataat
     8341 ttgacgagtg tattcgtacc aaaaatcata acatcgacat atttgtatca tgactttgat
     8401 tttgcaacgg aagtgattga tactttagtt gatgaagata aaggttgtcc atgggataaa
     8461 gtgcaaacgc atgaaacgct taagcgttat ttacttgaag aaacatttga attgttcgaa
     8521 gctattgaca atgaagatga ttggcatatg attgaagagc taggagatat tttattacaa
     8581 gtgttattgc atactagtat tggtaaaaaa gaagggtata tcgacattaa agaagtgatt
     8641 acaagtctta atgctaaaat gattcgtaga cacccacaca tatttggtga tgccaatgct
     8701 gaaactatcg atgacttaaa agaaatttgg tctaaggcga aagatgctga aggtaaacag
     8761 ccaagagtta aatttgaaaa agtatttgca gagcattttt taaatttata tgagaagacg
     8821 aaggataagt catttgatga ggccgcgtta aagcagtggc tagaaaaagg ggagagtaat
     8881 acatgagatt agataaatat ttaaaagtat cacggttaat aaagcgacgt acgctagcaa
     8941 aagaggtaag tgatcaaggt agaattacaa taaatggtaa tgttgctaaa gctggatcgg
     9001 atgttaaagt tgaagatgtg ctgacgattc gctttggtca aaaattagta acagttaaag
     9061 taactgcatt aaatgaacat gcatctaaag ataacgcgaa gggtatgtat gaaatcattg
     9121 aagagcgtcg acttgaagaa gcgtaaattg gaggtgacaa gcaatgaaaa ataaagtaga
     9181 acatatagaa aatcagtaca cgtcgcaaga gaacaagaaa aaacaacgtc aaaaaatgaa
     9241 aatgcgtgtt gttcgtaggc gtattacagt atttgcgggc gtattacttg cgataattgt
     9301 tgttttatca atcttgcttg ttgtccaaaa acatcgcaat gatattgatg cacaggagcg
     9361 aaaagcgaaa gaagcacagt ttcaaaagca acaaaatgaa gaaattgcgt taaaagaaaa
     9421 gttgaataat ctgaatgaca aagattacat tgaaaaaatt gcgcgtgatg attattactt
     9481 aagcaacaaa ggtgaagtga tttttaggtt gccagaagac aaagattcgt ctagctcaaa
     9541 atcttcgaaa aaataaatcc aaattgattc aaaattatcc gagtatagac attgtgaaaa
     9601 aatccaaaca aggatataat aagggaaaat cgaatcaaat cgggaggatt tatttaacat
     9661 atgtcaatcg aagttggaaa taagcttaaa ggtaaagtca ctggtattaa aaagtttggt
     9721 gcattcgtag aattacctga aggaaaaagt ggtttagttc acattagtga agtcgcagat
     9781 aattatgttg aaaacgtaga agagcacctt tctgttggtg atgaagtaga cgtgaaagta
     9841 ttatctattg ctgatgatgg aaaaattagt ctttcaatta agaaagctaa agaccgtcca
     9901 cgtagacaac atacgagtaa accaagtcat caaaaaccag tgcaaaaagc cgaagatttt
     9961 gaaaagaaat taagcaattt cttaaaagat agtgaagata aattaacttc aatcaaacgt
    10021 caaacagaat ctagacgcgg tggcaaaggt tctagacgtt aattaaaata aataaagact
    10081 gtttcgataa ggaatatatt tagaatgatg cgtatcgaat aatcgattgc agcgttagac
    10141 aatctaagac tgtttcttaa ataaggagca gtctctttta tttgtaatga tataactaag
    10201 acttatacca tttttgaaaa ttgtaaaagt gaggtgatgt tatgcagtta aatagtaatg
    10261 gttggcatgt tgatgaccat attgttgtcg ctgtttctac aggtattgat agtatgtgtt
    10321 tattgtatca actactaaat gattataaag atagttatag aaaactaaca tgcttacatg
    10381 tcaatcatgg cgttaggtca gcttcaattg aggaagccag atttttagaa gcatactgcg
    10441 aacgtcatca catcgattta catatcaaaa agttagattt gtcgcatagt ctcgaccgaa
    10501 ataacagcat tcagaatgaa gctcgaatta aacgttacga atggtttgat gaaatgatga
    10561 atgtattaga agcggatgta ttgctaacgg cgcatcattt ggacgatcaa ttagaaacta
    10621 ttatgtatcg tatttttaat gggaaatcaa cgcgtaataa actaggattt gatgagttat
    10681 cgaagcgaaa aggttatcag atttatcgac cacttttagc tgtctctaaa aaagaaataa
    10741 aacaattcca agagagatat catattccat attttgaaga tgaatctaat aaagataaca
    10801 aatatgttag aaatgatatt cgtaatagaa ttattccagc tattgatgaa aataatcaac
    10861 ttaaagtatc gcatttatta aaattaaaac aatggcatga tgaacaatat gatattttgc
    10921 aatattcagc taaacaattt attcaagaat ttgtgaagtt tgatgaacag tcaaaatatt
    10981 tagaggtttc tagacaagct tttaataact taccaaactc attaaagatg gtggtgttgg
    11041 actgcctatt atcaaagtat tatgagttgt ttaatattag tgctaaaaca tacgaagagt
    11101 ggtttaaaca atttagtagt aagaaagcac aattcagtat taatctcacg gataaatgga
    11161 taattcaaat cgcatatggt aaattaataa taatggctaa aaataatggc gatacatatt
    11221 ttagagttca aacaattaaa aagccaggta attatatttt taacaaatat cgattagaga
    11281 tacattctaa tttaccaaaa tgtttatttc cgcttacagt gagaacacga caaagtggcg
    11341 ataaatttaa actgaatggg cgcgatggtt ataagaaagt gaatcgcctg tttatagatt
    11401 gtaaagtgcc acagtgggtt cgggatcaaa tgccaatcgt attggataaa caacagcgca
    11461 ttattgcggt aggagattta tatcaacaac aaacaataaa aaaatggatt ataattagta
    11521 aaaatggaga tgaatagcgt tatgcataat gatttgaaag aagtattgtt aactgaagaa
    11581 gatattcaaa atatctgtaa ggaattggga gcacaattaa caaaggatta tcaaggtaaa
    11641 ccattagtat gcgtgggtat cttaaaaggc tcagcaatgt ttatgtcaga tttaattaaa
    11701 cgaattgata cccatttatc aattgatttc atggatgttt ctagttatca cggaggcact
    11761 gagtcaactg gtgaagttca aatcattaaa gatttaggtt cttctattga aaataaagac
    11821 gtattaatta ttgaagatat cttagagact ggtactacac ttaagtcaat tactgaatta
    11881 ttacaatcta gaaaagttaa ttcattagaa atagttactt tattagataa accaaaccgt
    11941 cgtaaagcgg acattgaagc taagtatgta ggtaaaaaaa taccagatga atttgttgtt
    12001 ggttacggtt tagattatcg tgaattatac cgaaacttac catatatcgg tacgttaaaa
    12061 cctgaagtgt attcaaatta attttttaat caatttcagt tattattact atgcgtttga
    12121 gaaataatag tgtagactca aaaatatgaa aaatgtattt catatatatt taattttaga
    12181 caagacatat gtcttgaaaa gttgaaaaat atagagattg ataaaactaa tacggatgtg
    12241 aatgacattg atgttaagct caattactag cttataaaac atgtcatatg ttacaatttt
    12301 tgttagtttt attatgggaa gtaggaggaa atgacgcatg cagaaagctt ttcgcaatgt
    12361 gctagttatc gtaataatag gcgttattat ttttggtcta ttttcatatt taaacggtaa
    12421 tggaaatatg ccgaaacagc ttacatataa tcaatttact gagaagttgg aaaaaggtga
    12481 ccttaaaact ttagaaatcc aaccacaaca aaatgtctat atggtaagtg gtaaaacgaa
    12541 aaatgatgaa gactattcat caactatttt atataacaac gaaaaagagt tacaaaaaat
    12601 tactgatgct gctaaaaagc aaaacggtgt aaaattaacg attaaagaag aagaaaaaca
    12661 aagtgtcttt gtgagtatac tttcaacatt aattccagtt gtagtcatag cgttattatt
    12721 tattttcttc ctaagccaag cacaaggtgg cggtagtggc ggtcgtatga tgaactttgg
    12781 taaatctaaa gcgaaaatgt acgataataa caaacgtcgt gttcgtttct ctgatgtagc
    12841 aggggcagat gaagaaaaac aagaattaat tgaaattgtt gatttcttga aagataataa
    12901 aaaattcaaa gaaatgggat ctaggattcc taaaggtgtc ttacttgttg gacctccagg
    12961 tactggtaaa acattacttg ctagagcggt tgcaggtgaa gcaggcgcac cattcttctc
    13021 tattagtggt tcagactttg tagagatgtt tgttggtgtt ggtgcgagcc gtgttcgtga
    13081 cttattcgat aatgctaaga aaaacgcgcc ttgtatcatc tttatcgatg agattgatgc
    13141 tgttggtcgt caacgtggtg caggtgttgg tggcggtcat gatgaacgtg aacaaaccct
    13201 aaaccaatta ttagttgaaa tggatggttt cggtgaaaat gaaggtatca ttatgatagc
    13261 tgctacaaac cgtcctgata tccttgaccc agccttatta cgtccaggtc gttttgatag
    13321 acaaattcaa gttggtcgtc cagatgtgaa aggccgtgaa gcaattcttc atgttcatgc
    13381 taaaaacaaa ccacttgatg aaacggttga tttaaaagca atttcacaac gtacacctgg
    13441 tttctcaggt gctgatttag agaacttatt aaatgaagca tctttaattg ctgtacgtga
    13501 aggtaaaaag aaaattgaca tgagagatat cgaagaggca acggatagag ttatagccgg
    13561 acctgctaag aaatctcgag ttatttctaa gaaagaacgt aatattgttg ctcatcacga
    13621 agctggtcat acaattatcg gtatggtact tgatgaggca gaagtagtgc ataaagttac
    13681 tattgttcca cgtggacaag caggtggtta tgcaatgatg ctacctaaac aagatcgttt
    13741 cttaatgact gaacaagagt tattagataa aatctgtggt ttacttggtg gacgtgtatc
    13801 agaagatatt aactttaacg aagtatcaac aggtgcttca aatgacttcg aacgtgcaac
    13861 acaaatcgca cgctcaatgg ttacgcaata tggtatgagt aaaaaattag gaccattaca
    13921 gttcggtcat agcaatggtc aagtattctt aggtaaagat atgcaaggtg agcctaatta
    13981 ttcaagccaa atcgcatatg aaattgataa agaagttcaa cgaatcgtta aagaacaata
    14041 cgaacgttgt aaacaaattt tattagagca caaagaacaa ttaattttaa ttgctgaaac
    14101 attattaaca gaagaaacat tagttgctga acaaattcaa tcattattct acgaaggtaa
    14161 attacctgaa attgattatg atgcagctaa agttgttaaa gatgaagatt ctgaatttaa
    14221 tgatggtaaa ttcggtaaat cttatgaaga gattcgtaaa gagcaattag aagatggaca
    14281 acgtgacgaa agtgaagatc gtaaagaaga aaaagatatt gctgaggata aaaaagaagc
    14341 tgataaatct gatgaaaaag atgaaccagc acatcgacaa gccccaaata tcgaaaaacc
    14401 ttacgatcca aatcacccag acaataaata atcgattata ttcagtacct ctttctatga
    14461 taaagttata gaaaggggta cttttatcgt ttttgaaaat acgtattaga ttttaagtcg
    14521 ttgaattgtt atagcagaaa ataattgtaa aacaagttac ttcattattt agaatgatgg
    14581 gtgtagaata agaacaattg ttgcatttta tgaagtaaag taatttttta aatatagagt
    14641 aatagaggag attgaaataa tgacacacga ttatattgtt aaagcattag catttgatgg
    14701 agagattagg gcttatgctg ctttgacaac tgaaactgtt caagaagcac aaacgagaca
    14761 ttatacatgg ccgacagcat ctgctgcaat gggaagaaca atgacagcaa cagctatgat
    14821 gggcgcaatg ttgaaaggtg atcaaaaatt aactgtcact gtagatggcc aaggacctat
    14881 tggacgaatt attgccgatg caaatgctaa aggcgaggtg cgtgcttatg tagaccatcc
    14941 acaaactcat tttccattaa atgagcaagg taaacttgat gtaagacgag cggtagggac
    15001 aaatggatct attatggttg ttaaagacgt tggaatgaaa gactatttct ctggagcaag
    15061 tccaattgtt tcaggagaac ttggtgaaga ttttacttat tattatgcta caagtgaaca
    15121 aacaccttca tcggtaggtc ttggtgtatt ggtaaatcct gataatacga ttaaagcagc
    15181 aggaggattt atcattcaag ttatgccagg tgccaaagat gaaacaattt caaaattaga
    15241 aaaagcaatt agtgaaatga caccagtttc taaattaatt gaacaaggat taacgccaga
    15301 aggattacta aacgaaatct taggtgaaga ccatgtgcaa attttagaga aaatgcctgt
    15361 tcaatttgaa tgtaattgta gtcatgagaa atttttaaat gctattaaag gattgggcga
    15421 ggctgagatt caaaatatga ttaaagaaga tcatggtgct gaagcagtat gtcatttctg
    15481 tggaaataaa tataaatata ctgaagaaga attaaacgtg ttgctagaaa gtttagcgta
    15541 atttaattta aatcaatacg ctaaaatgtt tatttttagc ggtttagtga aatgtagaac
    15601 taaatagttg tataatcctt agtgattttg tttgctttct agaatttatt tgataaaata
    15661 attctatatc cgataaataa actaagattt caacaactaa ctaaaaagga gtgttcttaa
    15721 tggcacgaaa accagtagat aatattactc aaattattgg cggtacaccg gtagtcaaat
    15781 tgagaaatgt agtagatgac aatgcagcag atgtttatgt aaaattggaa tatcaaaatc
    15841 caggtggttc tgtaaaggat agaattgctt tagcaatgat tgaaaaagca gagcgagaag
    15901 gcaaaattaa acctggcgat acaattgtag aaccaacaag tggtaataca ggtatcggtt
    15961 tagcatttgt atgtgctgct aaaggatata aagcagtatt tactatgccc gaaacaatga
    16021 gccaagagcg tcgtaattta ttaaaagcat acggtgcgga attagtttta acgcctggat
    16081 cagaagcgat gaaaggtgca attaaaaaag ctaaagaatt gaaagaagaa catggttact
    16141 tcgagccaca acaatttgaa aaccctgcga accctgaagt tcatgagtta actacaggtc
    16201 ctgagttatt acaacaattt gaagggaaaa ctatcgatgc gttcctagct ggtgttggta
    16261 ctggtggtac gttatctggt gtaggtaaag ttctgaaaaa agaatatcct aacatcgaaa
    16321 ttgttgctat agagcctgag gcttctccag tattgagcgg tggtgagcca ggtccacata
    16381 aattacaagg tttaggtgct ggatttattc caggcacttt gaatacagaa atctatgaca
    16441 gtattattaa agtaggaaat gatacagcga tggaaatgtc tcgtcgagtt gctaaagagg
    16501 aaggtatttt agcaggtatt tcatcaggtg ctgcgattta tgctgccatt caaaaagcaa
    16561 aagaattagg aaaaggaaaa acagtagtaa cagtattgcc gagtaatggt gaacgctact
    16621 tatcaacacc tttatattca ttcgatgact aattaatgtc atttaaaaga gtgagttatc
    16681 tttttg
//
LOCUS       DAHHYJ010000052        16676 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000052 DAHHYJ010000000
VERSION     DAHHYJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16676
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.052"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(123..962)
                     /gene="noc"
                     /locus_tag="NBK22_001253"
     CDS             complement(123..962)
                     /gene="noc"
                     /locus_tag="NBK22_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCD2414446.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
     gene            complement(1005..1724)
                     /gene="rsmG"
                     /locus_tag="NBK22_001254"
     CDS             complement(1005..1724)
                     /gene="rsmG"
                     /locus_tag="NBK22_001254"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCD2414447.1"
                     /translation="MTVEWLAEQLKEHNIQLTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSIAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            complement(1724..3601)
                     /gene="mnmG"
                     /locus_tag="NBK22_001255"
     CDS             complement(1724..3601)
                     /gene="mnmG"
                     /locus_tag="NBK22_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCD2414448.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            complement(3668..5047)
                     /gene="mnmE"
                     /locus_tag="NBK22_001256"
     CDS             complement(3668..5047)
                     /gene="mnmE"
                     /locus_tag="NBK22_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCD2414449.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADKLYKGKHLLND
                     VPSHTINYGHIIDPESKEVIEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            complement(5186..5539)
                     /gene="rnpA"
                     /locus_tag="NBK22_001257"
     CDS             complement(5186..5539)
                     /gene="rnpA"
                     /locus_tag="NBK22_001257"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCD2414450.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRVIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            complement(5660..5797)
                     /gene="rpmH"
                     /locus_tag="NBK22_001258"
     CDS             complement(5660..5797)
                     /gene="rpmH"
                     /locus_tag="NBK22_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCD2414451.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            6528..7889
                     /gene="dnaA"
                     /locus_tag="NBK22_001259"
     CDS             6528..7889
                     /gene="dnaA"
                     /locus_tag="NBK22_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCD2414452.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            8167..9300
                     /gene="dnaN"
                     /locus_tag="NBK22_001260"
     CDS             8167..9300
                     /gene="dnaN"
                     /locus_tag="NBK22_001260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCD2414453.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGSLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            9681..9926
                     /gene="yaaA"
                     /locus_tag="NBK22_001261"
     CDS             9681..9926
                     /gene="yaaA"
                     /locus_tag="NBK22_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCD2414454.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            9923..11035
                     /gene="recF"
                     /locus_tag="NBK22_001262"
     CDS             9923..11035
                     /gene="recF"
                     /locus_tag="NBK22_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCD2414455.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            11045..12979
                     /gene="gyrB"
                     /locus_tag="NBK22_001263"
     CDS             11045..12979
                     /gene="gyrB"
                     /locus_tag="NBK22_001263"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCD2414456.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANKIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNEIIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIKITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            13016..15679
                     /gene="gyrA"
                     /locus_tag="NBK22_001264"
     CDS             13016..15679
                     /gene="gyrA"
                     /locus_tag="NBK22_001264"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCD2414457.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDSSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTVSEDGTEQQREAVVNDETPGNAIHTEVIDSE
                     ENDEDGRIEVRQDFMNRVEEDIQQSSDEDEE"
     gene            complement(15767..16579)
                     /locus_tag="NBK22_001265"
     CDS             complement(15767..16579)
                     /locus_tag="NBK22_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCD2414458.1"
                     /translation="METLNSINIPKRKEDSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
BASE COUNT         5527 a         2835 c         2862 g         5452 t
ORIGIN      
        1 ccagcctgat caacatataa atataaattt ttatgtttca cgtaaaacaa aaaaagacaa
       61 agctgttatg atcttagctt tgtcttttat gtgttagtca tcatgtatac gacatcctac
      121 tactaacgtt tatatattcg aatttttatt tcataataat cttcatgatc tttgtcttta
      181 tgctcaacat gtagtcctgt ttgttgaatc gcttggatac ttttgcctac ctcatctcgt
      241 gcttgagtga catcttgtgc aaagcgaagg ttttgtgctt tgactttttc gggccccgtt
      301 ttttggcgta ctctatcttc agtctgtttc acatttagtt tttgtgcaat gacttgctca
      361 atcaacgctt cttgttcact atcagacaat gataataccg ctctcgcatg gcgttcagta
      421 attttacctt ctcttaagcg aagtagtact ttcggcgcca acttcaataa acgcaactta
      481 tttgcaataa agctttgact tttacctaaa ctttttgcca attcactttg cgttgtatca
      541 ccaatttcca ataatttctt ataggcttcc gcttcttcaa caacagacaa attttctctt
      601 tgaatattct caattaatgc aacaacagcc gtctcttcat catccatatc acgaataata
      661 acgtctgctt gaggtaaatt tagtgattgt attgctctaa atcggcgctc tccagcaata
      721 atttcaaaca tatcttcttc aatcggtctt acaacaatag gttgtagtaa accatgttca
      781 tgtattgatt cagcaagttc tttaatttta tttggttcaa acacctgtct tggttgataa
      841 cggttgggaa cgatacgttc aatttgaatg gattcaacat tactattgcg atcttcttca
      901 atatgtccaa tgatgtcatc tttgtttttc aaaccaaata attttgaaaa aggttttttc
      961 attatccatt cgctcctttt atgttagttg taaaggatta tgcattattt ttcaagtaaa
     1021 ggagtcttat taggcgtccc tggttttctt ggatactttt tcggcgtctg tcttttttta
     1081 tcaataatga acatctggcg ctctccagca tcttctggca attcgaaagt atgtgtttct
     1141 gtaacattac cacctaacac actaattgca aattttgctt cttctaattc ttcttcacct
     1201 tttgaagatt ttaatgcaac aaactgtcca ccttttttaa ctagcggtaa acacaattca
     1261 cttaacacgg ataatctagc tactgctctt gcagtaacaa catcataaga ctccctgtag
     1321 acacccttac caaatgtttc tgctctatcg tgtataaagc tgacatcctg taattgtaat
     1381 tctgacgcta aatggtttaa aaattgaata cgcttattta atgaatcaac aatcgtcact
     1441 tttaactgcg gaaacattat ttttaacgga atacttggaa aaccagctcc agcgcctaca
     1501 tcacatatac ttataggctg attaaaatca aaataaaaac taggtgcaat ggaatcataa
     1561 aaatgtttca aatatacatc gtgttcatct gtaatacttg tcaaattcat cttttcattc
     1621 cattcaacaa gtaaacgata atatgtttga aactgttgtt tttgagtctc agttaattga
     1681 atattatgtt cttttaattg ttctgctaac cattctacag tcattagtca ctcacccttt
     1741 ggagtttacc ttgttctaag taaatcaata atatagatat gtctgctgga tttacccctg
     1801 atattctaga agcttgtgca atatttaaag gttttacttc tgataatttt tctcgcgctt
     1861 cagtcgccaa actatcaatc ttactataat ctaagtcttc tggaattttc ttctcttcca
     1921 tacgcttaac tttctcaact tgttgtagtg atttattgat ataaccttca tattttgttt
     1981 gtatttctac ttgttcttca acatctgcat tcaattgatg ttcttcttct aaaatttcta
     2041 aaattatatc gtaagtcatt tcaggtctgc gtaataaatc gatagctaaa ataccatctt
     2101 ttaagcgaga accaccatgt tgttcgataa tcgcttgcgt atgttcgttt ggtttaatac
     2161 gaatatctga taaacgctta atttccgcat caatttgctg acgtttttca ttaaaacgtg
     2221 catatcgttc ttcagaaatc ataccaagtt catatcccat atccgtcaat ctcaaatcag
     2281 cattatcatg acgtagtaac aaacgatatt ctgcacgtga tgttagtaaa cgataaggtt
     2341 cattagtacc tttagttaca agatcatcga ttaagacacc aatatatgca tctgaacgac
     2401 ttaatatctt ttcgcctgtg tttaacactt tacctgcagc gttaatacct gccatcaatc
     2461 cttgtcctgc tgcttcttca taaccagatg taccattaat ttgacctgca gtatataagt
     2521 ttttaatcat tttcgtttca agtgtaggcc ataactgcgt tggcacaatc gcatcatatt
     2581 caattgcgta gccggcacgc atcatatctg ctttttcaag acctggtatc gtctctaaca
     2641 tttgacgttg cacatgttca ggaagacttg tagataatcc ttgcacatat acttcatttg
     2701 tattacgacc ttcaggctct aagaaaagtt gatgtcgcgg cttatcatta aatcgaacaa
     2761 atttatcttc aattgaaggg caataacgtg gcccggttcc tttaatcatc cctgaataca
     2821 ttgcagatag atgtaaatta tcatcgataa ctttgtgtgt ttcagcatta gtatacgtta
     2881 gccaacatgg caattgatct aatatatatt ctgttgtttc aaagctgaat gcacgaccta
     2941 catcgtcacc tggttgtatt tcagtcttcg aatagtcaat tgtttttgaa tttacacgcg
     3001 gtggtgtacc tgttttaaaa cgaacaatat caaagccaag ttctcttaaa ttgtctgata
     3061 atgtgattga tggtaattgg tgatttggtc cacttgaata cttcatatta cctaaaatga
     3121 tttcaccacg taaaaatgtt cccgttgtaa taattactgc tttagataaa tactctgtac
     3181 caatatttgt acgtacacct ttaacttcat tatcttctat aataagttcg tctaccatac
     3241 cttgcattat atgcaaattt tcttcatctt caatcacgcg tttcatttct tgttgataaa
     3301 gtactttatc tgcttgcgct cttagtgctc ttacagcagg tcctttacct gtatttaaca
     3361 ttctcatttg aatgtgtgtt ttatcgattg tttttgccat ttgtccacct aaagcatcaa
     3421 tttcacgaac aacgatgcct ttagctggtc cacctacaga tgggttacat ggcataaatg
     3481 caatattatc taaatttatt gttagcatta atgttttagc accacgtctt gcagatgcta
     3541 aacctgcttc tacacctgca tgtcccgcac ctataacgat tacatcatat tcttgaacca
     3601 caatataaac ctccttattt gatatcttac tagccttttt aagacggtat tccgtctatt
     3661 tcaattacta tttacctaag cagaattgac tgaataactg atcgatgagt tcatcacttg
     3721 cagtctcacc aataatttct cctaatattt cccaagttct agttagatca atttgtacca
     3781 tatccatagg cacaccagat tctgctgcat caatcgcatc ttgtatcgtt tgtcttgctt
     3841 gttttaataa tgaaatatgt cttgaattag aaacataagt catatcttga ttttgtactt
     3901 ctccaccaaa gaacaaatct cgaatttgta tttctaattc atcaatacct tcttgtttta
     3961 acattgaagt ttgaattaat ggcgtatcac ctatcatatc tttaacttca ttaatatcta
     4021 tgttttgctc taaatccatt ttattaacaa ttacgattac atcttcattt ttaaccactt
     4081 catataatgt gtaatcttct tgagtcaatg cttcgttatt gtttaataca aataaaatta
     4141 agtctgcttg gctaagagcc tttctagagc gttcaacacc aatcttctct actatatctt
     4201 ctgtctcacg tataccagca gtatcaacta atcttaatgg cacgccacga acattgacgt
     4261 actcttctaa gacatctcta gtcgtacctg ctacctcagt tacaatcgct ttattatctt
     4321 gtattaaatt atttaacatc gatgatttac ctacgtttgg tttaccaaca ataactgtag
     4381 ataaaccttc acgcataatt ttaccctgcg caccggtatc taataaacga ttaatttcct
     4441 gtttgatttc tttagactgc tctaaaagaa attcagtagt cgcatcttca acatcatcgt
     4501 attcaggata atcaatattc acttccactt gagcgagtat ctctaatata gattgacgtt
     4561 gttttttgat taagtcactt agacgacctt caatttgatt catcgcaact ttagaagctc
     4621 tatctgtctt cgagcgaata aagtccataa ctgcttcagc ttgagataaa tcaatacgac
     4681 catttaaaaa ggcacgtttt gtaaattcac ctggctcagc cattctagcg ccatatgtca
     4741 tagtaagttc cagcactcta ttaatcgtta aaataccacc atgacaatta atttctataa
     4801 tatcttcgcg tgtaaatgtt tttggcgctc ttaacacaga caccataact tcttcaatca
     4861 cttctttaga ctctggatca ataatatgac cgtaattaat cgtatgtgat ggaacatcat
     4921 ttaaaagatg ttttccttta tataatttgt cagcaatttc aacggcttgc ggtccagaca
     4981 atcgaacaat tccaattgcc ccttcaccca ttggtgttga aatactcgta attgtatcta
     5041 aatccatatt gctactcgcc tccttcaacg acgtgaatac attttaaaat aagttattat
     5101 aacccagagg taagtcttac gtttgtctga ggtaagactt cgggatgtgt tgagtggtta
     5161 atgtttttct tcccctaccc tatccttact taattttttt attaaaaact ttggcaattt
     5221 taagtacgtg ctcaagacta ttctgtattt gtaaagtcgt catatcttta gctggctgtc
     5281 ttgctattac aataatatct ttggccaata tatgcgactt atgtactttg aaattttcac
     5341 gtattactct tttaatcttg tttcttaaca ctgcattacc tagtttttta gaaacactaa
     5401 tacctaagcg aaaatggtct atttctttat tattacaagt gtatacaaca aattgtctgt
     5461 tggctacaga atgacctttt ttatatattc tctgaaaatc tgcattcttt ttaattcggt
     5521 aagctttttc caataacatc actcgcttat ttatcgtttt tatttgaagc tatatttaaa
     5581 cttctattga gcttataaca taaatttcta tttattctta atttaaacga aaaaaaagat
     5641 cactgatgag tcagtgatct tatgcagata aaactttacg gcctttacga cgacggcgcg
     5701 ctaaaacttt acggccattt tttgtgctca tgcgttttct gaaaccatga actttactat
     5761 gtttacgttt atttggttga taagtacgtt ttaccatgca aaacacctcc atctttatac
     5821 agttatctaa tataccagat tatgcagact ctgatcactt taatagataa gattttgcgt
     5881 ttcttagatt aaaattctcg tattagctca ttgattatct agtcataatt caagcaacta
     5941 ctacaatata acaaaatcct ttttataacg caagttcatt ttatactact gctcaatttt
     6001 tttactttta tcgattaaag atagaaatac acgatgcgag caatcaaatt tcataacatc
     6061 accatgagtt tggtccgaag catgagtgtt tacaatgttt gaatacctta tacagttctt
     6121 atacatactt tataaattat ttcccaagct gttttgatac actcactaac agatattcta
     6181 tagaaggaaa agttatccac ttatgcacat ttatagtttt cagaattgtg gataattaga
     6241 aattacacac aaagttatac tatttttagc aacatattca caggtatttg acatatagag
     6301 aactgaaaaa gtataattgt gtggataagt cgtccaactc atgattttat aaggatttat
     6361 ttattgatat ttacataaaa atactgtgca taactaataa gcaggataaa gttatccacc
     6421 gattgttatt aacttgtgga taactattaa catggtgtgt ttagaagtta tccacggctg
     6481 ttatttttgt gtataactta aaaatttaag aaagatggag taaatttatg tcggaaaaag
     6541 aaatttggga aaaagtgctt gaaattgctc aagaaaaatt atcagctgta agttactcaa
     6601 ctttcctaaa agatactgag ctttacacga ttaaagatgg tgaagctatc gtattatcga
     6661 gtattccttt taatgcaaat tggttaaatc aacaatatgc tgaaattatc caagcaatct
     6721 tatttgatgt tgtaggctat gaagttaaac ctcactttat tactactgaa gaattagcaa
     6781 attatagtaa taatgaaact gctactccaa aagaaacaac aaaaccttct actgaaacaa
     6841 ctgaggataa tcatgtgctt ggtagagagc aattcaatgc ccataacaca tttgacactt
     6901 ttgtaatcgg acctggtaac cgctttccac atgcagcaag tttagctgta gccgaagcac
     6961 cagccaaagc gtacaatcca ttatttatct atggaggtgt tggtttagga aaaacccatt
     7021 taatgcatgc cattggtcat catgttttag ataataatcc agatgccaaa gtgatttaca
     7081 catcaagtga aaaattcaca aatgaattta ttaaatcaat tcgtgataac gaaggtgaag
     7141 ctttcagaga aagatatcgt aatatcgacg tcttattaat cgatgatatt cagttcatac
     7201 aaaataaagt acaaacacaa gaagaatttt tctatacttt taatgaattg catcagaata
     7261 acaagcaaat agttatttcg agtgatcgac caccaaagga aattgcacaa ttagaagatc
     7321 gattacgttc acgctttgaa tgggggctaa ttgttgatat tacgccacca gattatgaaa
     7381 ctcgaatggc aattttgcag aagaaaattg aagaagaaaa attagatatt ccaccagaag
     7441 ctttaaatta tatagcaaat caaattcaat ctaatattcg tgaattagaa ggtgcattaa
     7501 cacgtttact tgcatattca caattattag gaaaaccaat tacaactgaa ttaactgctg
     7561 aagctttaaa agatatcatt caagcaccaa aatctaaaaa gattaccatc caagatattc
     7621 aaaaaattgt aggccagtac tataatgtta gaattgaaga tttcagtgca aaaaaacgta
     7681 caaagtcaat tgcatatccg cgtcaaatag ctatgtactt gtctagagag cttacagatt
     7741 tctcattacc taaaattggt gaagaatttg gtgggcgtga tcatacgacc gtcattcatg
     7801 ctcatgaaaa aatatctaaa gatttaaaag aagatcctat ttttaaacaa gaagtagaga
     7861 atcttgaaaa agaaataaga aatgtataag taggaaactt tgggaaatgt aatctgttat
     7921 ataacagcac taatgataac aatcattttt tacatttcta tatgctaatg tggcaagatg
     7981 agcaaaactc attttgtgga taatgtttaa aagtcataca caccatacac aagttatcaa
     8041 catgtgtata acttcgccaa atctatgttt ttaagactta tccaccaatc cacagcacct
     8101 actactatta ctaagaactt aaaacctata taattatata taaacgactg gaaggagttt
     8161 taattaatga tggaattcac tattaaaaga gattatttta ttacacaatt aaatgacaca
     8221 ttaaaagcta tttcaccaag aacaacatta cctatattaa ctggtatcaa aatcgatgcg
     8281 aaagaacatg aagttatatt aactggttca gactctgaaa tttcaataga aatcactatt
     8341 cctaaaactg tagatggcga agatattgtc aatatttcag aaacaggctc agtagtactt
     8401 cctggacgat tctttgttga tattataaaa aaattacctg gtaaagatgt taaattatct
     8461 acaaatgaac aattccagac attaattaca tcaggtcatt ctgaatttaa tttaagtggc
     8521 ttagatccag atcaatatcc tttattacct caagtttcta gagatgacgc aattcaattg
     8581 tcggtaaaag tgcttaaaaa cgtgattgca caaacaaatt ttgcagtgtc cacctcagaa
     8641 acacgcccag tactaactgg tgtgaactgg cttatacaag aaaatgaatt aatatgcaca
     8701 gcgactgact cacaccgctt ggctgtaaga aagttgcagt tagaagatgt ttctgaaaac
     8761 aaaaatgtca tcattccagg taaggcttta gctgaattaa ataaaattat gtctgacaat
     8821 gaagaagaca ttgatatctt ctttgcttca aaccaagttt tatttaaagt tggaaatgtg
     8881 aactttattt ctcgattatt agaaggacat tatcctgata caacacgttt attccctgaa
     8941 aactatgaaa ttaaattaag tatagacaat ggggagtttt atcatgcgat tgatcgtgcc
     9001 tctttattag cgcgtgaagg tggtaataac gttattaaat taagtacagg tgatgacgtt
     9061 gttgaattgt cttctacatc accagaaatt ggtactgtaa aagaagaagt tgatgcaaac
     9121 gatgttgaag gtggtagcct gaaaatttca ttcaactcta aatatatgat ggatgcttta
     9181 aaagcaatcg ataatgatga ggttgaagtt gaattcttcg gtacaatgaa accatttatt
     9241 ctaaaaccaa aaggtgacga ctcggtaacg caattaattt taccaatcag aacttactaa
     9301 aaataaatat aaataaagga tgacgtgatt aattaaaacg tcatccttta ttttttggca
     9361 aaaataattc taggtgcgta tgtaaaataa atttggcagc attttaaaca gcaaataaaa
     9421 gacgccaatt aaatttatga caaatgtatc caaaatttaa taagtgtgct tatatgccct
     9481 ttaaatttaa aattttaata gtcaataaca agttgaatat aaaagttaaa cgccgttaaa
     9541 tagcgttaaa aaattgaaaa tgacagtatt gccaaaaaat aagaattaat tatttatatg
     9601 taaacggttt ctacctctat tttaaatgaa atttgtgaca aaaaaaggta taatatatta
     9661 atgacataca aagaaatgga gtgattattt tggttcaaga agttgtagta gaaggagaca
     9721 ttaatttagg tcaatttcta aaaacagaag ggattattga atctggtggt caagcaaaat
     9781 ggttcttgca agacgttgaa gtattaatta atggagtgcg tgaaacacgt cgcggtaaaa
     9841 agttagaaca tcaagatcgt atagatatcc cagaattacc tgaagatgct ggttctttct
     9901 taatcattca tcaaggtgaa caatgaagtt aaatacactc caattagaaa attatcgtaa
     9961 ctatgatgag gttacgttga aatgtcatcc tgacgtgaat atcctcattg gagaaaatgc
    10021 acaaggaaag acaaatttac ttgaatcaat ttatacctta gctttagcaa aaagtcatag
    10081 aacgagtaat gataaggaac tcatacgttt taatgctgat tatgctaaaa tagaaggtga
    10141 gcttagttat agacacggca cgatgccatt aacaatgttt ataactaaaa aaggtaaaca
    10201 agtcaaagtg aatcacttag agcaaagtcg tctaactcaa tatattggac acctcaatgt
    10261 ggttctattt gcgccagaag atttgaatat tgtaaaaggc tctcctcaaa taagacgacg
    10321 ctttatagat atggagttgg gccaaatttc tgctgtttac ttaaatgatt tagctcaata
    10381 ccaacgtatt ttaaagcaaa agaataatta cttaaagcag ttacaattag gccaaaaaaa
    10441 ggacttaaca atgttggaag tattaaatca gcagtttgct gaatatgcaa tgaaagtaac
    10501 tgataaacgt gcacatttta ttcaagagct agagtcgtta gctaaaccga ttcatgctgg
    10561 tatcacaaat gataaagaag cgttgtcgct gaattattta cctagtctta aatttgatta
    10621 tgctcaaaat gaagcggcac gacttgaaga aattatgtct attcttagcg ataatatgca
    10681 aagagaaaaa gaacgaggca ttagcttatt cggaccacat cgagatgata taagttttga
    10741 tgtgaatggc atggatgctc aaacatatgg ttctcaagga cagcaacgta caacggcttt
    10801 gtccattaaa ttagctgaaa ttgagttaat gaatatcgaa gttggggaat atcccatctt
    10861 attattagac gatgtactca gtgaattaga tgattcgcgt caaacgcatt tattaagtac
    10921 gattcagcat aaagtacaaa catttgtcac tacgacatct gtagatggta ttgatcatga
    10981 aatcatgaat aacgctaaat tgtatcgtat taatcaaggt gaaattataa agtaacagaa
    11041 agcgatggtg actgcattgt cagatgtaaa caacacggat aattatggtg ctgggcaaat
    11101 acaagtatta gaaggtttag aagcagtacg taaaagacca ggtatgtata taggatcgac
    11161 ttcagagaga ggtttgcacc atttagtgtg ggaaattgtc gataatagta tcgatgaagc
    11221 attagctggt tatgcaaata aaattgaagt tgttattgaa aaagataact ggattaaagt
    11281 aacggataac ggacgtggta tcccagttga tattcaagaa aaaatgggac gtccagctgt
    11341 cgaagttatt ttaactgttt tacatgctgg tggtaaattc ggcggtggcg gatacaaagt
    11401 atctggtggt ttacatggtg ttggttcatc agttgtaaac gcattgtcac aagacttaga
    11461 agtatatgta cacagaaatg agattatata tcatcaagca tataaaaaag gtgtacctca
    11521 atttgactta aaagaagttg gcacaactga taagacaggt actgtcattc gttttaaagc
    11581 agatggagaa atcttcacag agacaactgt atacaactat gaaacattac agcagcgtat
    11641 tagagagctt gctttcttaa acaaaggaat taaaatcaca ttaagagatg aacgtgatga
    11701 agaaaacgtt agagaagact cctatcacta tgagggcggt attaaatcgt acgttgagtt
    11761 attgaacgaa aataaagaac ctattcatga tgagccaatt tatattcatc aatctaaaga
    11821 tgatattgaa gtagaaattg cgattcaata taactcagga tatgccacaa atcttttaac
    11881 ttacgcaaat aacattcata cgtatgaagg tggtacgcat gaagacggat tcaaacgtgc
    11941 attaacgcgt gtcttaaata gttatggttt aagtagcaag attatgaaag aagaaaaaga
    12001 tagactttct ggtgaagata cacgtgaagg tatgacagca attatatcta tcaaacatgg
    12061 tgatcctcaa ttcgaaggtc aaacgaagac aaaattaggt aattctgaag tgcgtcaagt
    12121 tgtagataaa ttattctcag agcactttga acgattttta tatgaaaatc cacaagtcgc
    12181 acgtacagtg gttgaaaaag gtattatggc ggcacgtgca cgtgttgctg cgaaaaaagc
    12241 gcgtgaagta acacgtcgta aatcagcgtt agatgtagca agtcttccag gtaaattagc
    12301 cgattgctct agtaaaagtc ctgaagaatg tgagattttc ttagtcgaag gggactctgc
    12361 cggggggtct acaaaatctg gtcgtgactc tagaacgcag gcgattttac cattacgagg
    12421 taagatatta aatgttgaaa aagcacgatt agatagaatt ttgaataaca atgaaattcg
    12481 tcaaatgatc acagcatttg gtacaggaat cggtggcgac tttgatctag cgaaagcaag
    12541 atatcacaaa atcgtcatta tgactgatgc cgatgtggat ggagcgcata ttagaacatt
    12601 gttattaaca ttcttctatc gatttatgag accgttaatt gaagcaggct atgtgtatat
    12661 tgcacagcca ccgttgtata aactgacaca aggtaaacaa aagtattatg tatacaatga
    12721 tagggaactt gataaactta aatctgaatt gaatccaaca ccaaaatggt ctattgcacg
    12781 atacaaaggt cttggagaaa tgaatgcaga tcaattatgg gaaacaacaa tgaaccctga
    12841 gcaccgcgct cttttacaag taaaacttga agatgcgatt gaagcggacc aaacatttga
    12901 aatgttaatg ggtgatgttg tagaaaaccg tagacaattt atagaagata atgcagttta
    12961 tgcaaactta gacttctaag cgctgtgaac tgaacttttg aaggaggaac tcttgatggc
    13021 tgaattacct caatcaagaa taaatgaacg aaatattacc agtgaaatgc gtgaatcatt
    13081 tttagattat gcgatgagtg ttatcgttgc tcgtgcattg ccagatgttc gtgacggttt
    13141 aaaaccagta catcgtcgta tactatatgg attaaatgaa caaggtatga caccggataa
    13201 atcatataaa aaatcagcac gtatcgttgg tgacgtaatg ggtaaatatc accctcatgg
    13261 tgactcatct atttatgaag caatggtacg tatggctcaa gatttcagtt atcgttatcc
    13321 gcttgttgat ggccaaggta actttggttc aatggatgga gatggcgcag cagcaatgcg
    13381 ttatactgaa gcgcgtatga ctaaaatcac acttgaactg ttacgtgata ttaataaaga
    13441 tacaatagat tttatcgata actatgatgg taatgaaaga gagccgtcag tcttacctgc
    13501 tcgattccct aacttgttag ccaatggtgc atcaggtatc gcggtaggta tggcaacgaa
    13561 tattccacca cataacttaa cagaattaat caatggtgta cttagcttaa gtaagaaccc
    13621 tgatatttca attgctgagt taatggagga tattgaaggt cctgatttcc ctactgctgg
    13681 acttatttta ggtaagagtg gtattagacg tgcatatgaa acaggtcgtg gttcaattca
    13741 aatgcgttct cgtgcagtta ttgaagaacg tggaggcgga cgtcaacgta ttgttgtcac
    13801 tgaaattcct ttccaagtga ataaggctcg tatgattgaa aaaattgcag agctcgttcg
    13861 tgacaagaaa attgacggta tcactgattt acgtgatgaa acaagtttac gtactggtgt
    13921 gcgtgtcgtt attgatgtgc gtaaggatgc aaatgctagt gtcattttaa ataacttata
    13981 caaacaaaca cctcttcaaa catcatttgg tgtgaatatg attgcacttg taaatggtag
    14041 accgaagctt attaatttaa aagaagcgtt ggtacattat ttagagcatc aaaagacagt
    14101 tgttagaaga cgtacgcaat acaacttacg taaagctaaa gatcgtgccc acattttaga
    14161 aggattacgt atcgcacttg accatatcga tgaaattatt tcaacgattc gtgagtcaga
    14221 tacagataaa gttgcaatgg aaagcttgca acaacgcttc aaactttctg aaaaacaagc
    14281 tcaagctatt ttagacatgc gtttaagacg tctaacaggt ttagagagag acaaaattga
    14341 agctgaatat aatgagttat taaattatat tagtgaatta gaagcaatct tagctgatga
    14401 agaagtgtta ttacagttag ttagagatga attgactgaa attagagatc gtttcggtga
    14461 tgatcgtcgt acagaaattc aattaggtgg atttgaagac ttagaggacg aagacttaat
    14521 tccagaagaa caaatagtaa ttactttgag ccataataac tacattaaac gtttgccggt
    14581 atctacatat cgtgctcaaa accgtggtgg tcgtggtgtt caaggtatga acacattgga
    14641 agaagatttt gtcagtcaat tggtaacttt aagtacacat gaccatgtat tgttctttac
    14701 taacaaaggt cgtgtataca aactaaaagg ttatgaagtg cctgagttat caagacagtc
    14761 taaaggtatt cctgtagtga atgctattga acttgaaaat gatgaagtca ttagtacaat
    14821 gattgctgtt aaagaccttg aaagtgaaga caacttctta gtgtttgcaa ctaaacgtgg
    14881 tgtcgttaaa cgttcagcat taagcaactt ctcaagaata aatagaaatg gtaagattgc
    14941 gatttcgttc agagaagatg atgagttaat tgcagttcgc ttaacaagtg gtcaagaaga
    15001 tatcttgatt ggtacatcac atgcatcatt aattcgattc cctgaatcaa cattacgtcc
    15061 tttaggccgt acagcaacgg gtgtgaaagg tattacactt cgtgaaggtg acgaagttgt
    15121 agggcttgat gtagctcatg caaacagtgt tgatgaagta ttagtagtta ctgaaaatgg
    15181 ttatggtaaa cgtacgccag ttaatgacta tcgtttatca aatcgtggtg gtaaaggtat
    15241 taaaacagct acgattactg agcgtaatgg taatgttgta tgtatcacta cagtaactgg
    15301 tgaagaagat ttaatgattg ttactaatgc aggtgtcatt attcgactag atgttgcaga
    15361 tatttctcaa aatggtcgtg cagcacaagg tgttcgctta attcgtttag gcgatgatca
    15421 atttgtttca acggttgcta aagtaaaaga agatgcagaa gatgaaacga atgaagatga
    15481 gcaatctact gtatctgaag atggtactga acaacaacgt gaagcggttg taaatgatga
    15541 aacaccagga aatgcaattc atactgaagt gattgattca gaagaaaatg atgaagatgg
    15601 acgtattgaa gtaagacaag atttcatgaa tcgtgttgaa gaagatatac aacaatcatc
    15661 agatgaagat gaagaataat aaaaaaataa gacttcccta tatgtaggga agtcttattt
    15721 ttatgctaaa aagtaatgct ttactatatt caatgattag taatgactaa ctttctaatt
    15781 gtttcattgc gtaaggtatt tcattgataa gtcttgatgg tggtaccaca tacatatctt
    15841 ttgcaaggtt ttcgccaata aaactatgtg tatatgtggc actcataacc gcttctttta
    15901 agttatcaaa ttgaccgaca aaacttgtaa tcataccagc aagtgtatcg cccataccac
    15961 cagtcgccat tgctgggcta ccgattgtta atttaaagtc ttcatcttta aagaaaattt
    16021 cagtaccatg ttttttaagt acaacagttg cgcctaaacg atcaactgct tcacgattac
    16081 gctcatatgt ctgttcctca ataggaatac cacttaatcg ctcccattct ttgagatgcg
    16141 gagtaaaaat cacgcgacat gtaggtaatt gcggtttcag tttactaaag attgtaatcg
    16201 catcgccgtc tacgattaaa ttttgatgcg gttgtatatt ttgtagtagg aatgtaatgg
    16261 cattatttcc tttgaaatca acgccaagac ctggaccaat tagtatactg tcagtcattt
    16321 caatcatttt cgtcaacatt ttcgtatcat taatatcaat aaccatcgct tctgggcaac
    16381 gagaatgtaa tgctgaatga tttgttggat gtgtagctac agtgattaaa ccactaccgc
    16441 taaatacaca tgcacgagcc gctaacataa tggcaccacc taagttagca gatccaccaa
    16501 ttaataaaat tttgccataa tcacctttat gtgaatcttc tttacgctta ggaatgttaa
    16561 tagaatttaa cgtttccata gtgatataac ctcccatgta aaagcctttt ccgaatttat
    16621 tcaattttaa aaatatatag taacttttaa caaaatgtat tataaatttc tgaatt
//
LOCUS       DAHHYJ010000053        16674 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000053 DAHHYJ010000000
VERSION     DAHHYJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16674
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.053"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            382..1719
                     /locus_tag="NBK22_001266"
     CDS             382..1719
                     /locus_tag="NBK22_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCD2414459.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLENKGFEIIQVDDPLKLLIKYDGFTGHDIQSWFMNNHIYFELADDYQALAILPLWH
                     HDDTYLFDLLLHKIEDMILPKKSFSKVKQTQLLTTEGNYKPKRFEYVTWRDLKKAQGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            1721..2338
                     /locus_tag="NBK22_001267"
     CDS             1721..2338
                     /locus_tag="NBK22_001267"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCD2414460.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            2366..2695
                     /locus_tag="NBK22_001268"
     CDS             2366..2695
                     /locus_tag="NBK22_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCD2414461.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            2909..3835
                     /locus_tag="NBK22_001269"
     CDS             2909..3835
                     /locus_tag="NBK22_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCD2414462.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIENTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQDMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            3836..4639
                     /locus_tag="NBK22_001270"
     CDS             3836..4639
                     /locus_tag="NBK22_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCD2414463.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRVADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            4656..5003
                     /gene="yabA"
                     /locus_tag="NBK22_001271"
     CDS             4656..5003
                     /gene="yabA"
                     /locus_tag="NBK22_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCD2414464.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            5277..6002
                     /locus_tag="NBK22_001272"
     CDS             5277..6002
                     /locus_tag="NBK22_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCD2414465.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYITMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            5995..6243
                     /locus_tag="NBK22_001273"
     CDS             5995..6243
                     /locus_tag="NBK22_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD2414466.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            6245..7084
                     /gene="rsmI"
                     /locus_tag="NBK22_001274"
     CDS             6245..7084
                     /gene="rsmI"
                     /locus_tag="NBK22_001274"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCD2414467.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            7369..9342
                     /gene="metG"
                     /locus_tag="NBK22_001275"
     CDS             7369..9342
                     /gene="metG"
                     /locus_tag="NBK22_001275"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCD2414468.1"
                     /translation="MVKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPAGIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            9373..10146
                     /locus_tag="NBK22_001276"
     CDS             9373..10146
                     /locus_tag="NBK22_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCD2414469.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            10313..10849
                     /gene="rnmV"
                     /locus_tag="NBK22_001277"
     CDS             10313..10849
                     /gene="rnmV"
                     /locus_tag="NBK22_001277"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCD2414470.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            10860..11753
                     /gene="rsmA"
                     /locus_tag="NBK22_001278"
     CDS             10860..11753
                     /gene="rsmA"
                     /locus_tag="NBK22_001278"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCD2414471.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            11853..12116
                     /locus_tag="NBK22_001279"
     CDS             11853..12116
                     /locus_tag="NBK22_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCD2414472.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            12426..13274
                     /locus_tag="NBK22_001280"
     CDS             12426..13274
                     /locus_tag="NBK22_001280"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCD2414473.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            13288..14112
                     /gene="purR"
                     /locus_tag="NBK22_001281"
     CDS             13288..14112
                     /gene="purR"
                     /locus_tag="NBK22_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCD2414474.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            14129..14509
                     /locus_tag="NBK22_001282"
     CDS             14129..14509
                     /locus_tag="NBK22_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCD2414475.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNVDGKIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLNSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            14581..14883
                     /gene="spoVG"
                     /locus_tag="NBK22_001283"
     CDS             14581..14883
                     /gene="spoVG"
                     /locus_tag="NBK22_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCD2414476.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            15073..15204
                     /locus_tag="NBK22_001284"
     CDS             15073..15204
                     /locus_tag="NBK22_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414477.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            15227..16579
                     /gene="glmU"
                     /locus_tag="NBK22_001285"
     CDS             15227..16579
                     /gene="glmU"
                     /locus_tag="NBK22_001285"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCD2414478.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVQMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
BASE COUNT         6056 a         2206 c         3347 g         5065 t
ORIGIN      
        1 aacttacgtt ccgctagagt agaacgttgc taggcaagaa atgaatgcga tgagccgcat
       61 tgagtgtgaa attgatattt taataatgtg cacttttgat tatttaaatc tgtgactaga
      121 agtataagtg aagacattca gaagtattat aaaaagtgaa cagcagtaag atagttttta
      181 atcataaatc atcttactgc tgtttttaga ttttatgtct aatatctttt aaatcgaagt
      241 acaaaaagga aattaattat tatacaatag acaagctatt gcataagtaa cactaacttt
      301 tgtcaaagaa gtgttactat ataattaata cttttgaaag taactaattc caaaacagtg
      361 taaataaagg aagcgtatat catgaagcaa cctattttaa ataaattaga aagtttaaat
      421 caagaagaag cgatttcttt gcatgttccg ggtcataaaa atatgactat tggtcattta
      481 tctcaattat caatgacaat ggataaaact gaaatacctg gattagatga tttacatcat
      541 cctgaagaag tcattttgga gagtatgaag caggtggaga agcattcaga ttatgatgct
      601 tatttcttag tgaatggcac cacttcagga atattatctg tcatccagtc tttttcacag
      661 aaaaaaggcg atatcttaat ggcaagaaat gtacataaat ctgtattaca tgcgctcgat
      721 attagccaac aagaagggca ttttattgaa acgcatcaaa gtccgttaac gaatcattat
      781 aataaagtta atttaagccg tttgaataat gacggtcaca aacttgctgt gttgacttat
      841 cctaactatt acggtgaaac atttaatgta gaagaggtta tcaaatcttt gcaccaatta
      901 aatattcctg tactcattga cgaagcacac ggcgcgcact ttggattgca aggatttcca
      961 gattctacat taaattatca agctgactat gttgttcaat cttttcataa aacgttacca
     1021 gctttaacga tgggttcggt actttatatt cataaaaatg caccttatag agaaactatt
     1081 atagaatatc taagctactt ccaaacatct agtccttcgt atttgattat ggctagttta
     1141 gagtcagctg ccgagttcta taaaacatat gatagtaccg tgttttttga taagagagca
     1201 caattaatcg aatgtttgga gaataaaggt tttgaaatta ttcaagtgga tgatccgttg
     1261 aagttactaa taaaatatga tggctttaca ggacatgata ttcaaagttg gtttatgaat
     1321 aatcatattt attttgagtt agcagacgac tatcaagcat tagcgatatt gccgttatgg
     1381 catcatgatg atacgtattt atttgatttg cttttacata aaattgaaga tatgatttta
     1441 ccgaaaaaat cattttctaa agttaaacaa acacaacttt taacaactga aggtaactat
     1501 aaaccaaaac gctttgaata tgttacttgg cgtgatttga aaaaggcaca aggtaaagtt
     1561 ctggcgcgac atattgtccc gtatccgcca gggattccta ttattttcaa aggagaaaca
     1621 ataactgaaa atatgataga attggtaaat gaatatctgg aaactggaat gatagttgaa
     1681 ggaattaaaa ataataaaat tttagttgag gatgaataaa atgtcagctt ttataacttt
     1741 tgagggccca gaaggctctg gaaaaacaac tgtaattaat gaagtttacc atagattagt
     1801 aaaagattat gatgtcatta tgacaagaga accgggcggc gttcctactg gtgaagaaat
     1861 acgtaaaatt gtattagaag gcaatgatat ggacattaga actgaagcaa tgttatttgc
     1921 tgcatctaga agagaacatc ttgtattaaa ggtcatacca gctttaaaag aaggtaaggt
     1981 tgtgttgtgt gatcgctata tcgatagttc attagcttat caaggttatg ctagagggat
     2041 tggcgttgaa gaagtaagag cattaaacga atttgcaata aatggattat atccagactt
     2101 gacgatttat ttaaatgtta gtgctgaagt aggtcgcgaa cgtattatta aaaattcaag
     2161 agatcaaaat agattagatc aagaagattt aaagtttcac gaaaaagtaa ttgaaggtta
     2221 ccaagaaatc attcataatg aatcacaacg gttcaaaagc gttaatgcag atcaacctct
     2281 tgaaaatgtt gttgaagaca cgtatcaaac tatcatcaaa tatttagaaa agatatgata
     2341 taattgttgg aagaggtgtt ataaaatgaa aatgattata gcgatcgtac aagatcaaga
     2401 tagtcaggaa cttgcagatc aacttgttaa aaataacttt agagcaacaa aattggcaac
     2461 aacaggtggg tttttaagag cgggtaatac aacattctta tgtggtgtca atgatgaccg
     2521 tgtagatgaa atattgtctg tgattaatca aacgtgtggt aatagagaac agttggtttc
     2581 acctattaca cctatgggag gcagtgcgga ttcgtacatt ccatatccag ttgaagttga
     2641 agttggcggt gctactgtat ttgttatgcc agttgatgca ttccatcaat tttaattcta
     2701 taatacaatc atcaattaga gatacttaaa atagtgtatt aataagttat taacaatttt
     2761 gggttgcttg actgcgacta gttcagatgc caatagattt gatttttgtg gttctaaaaa
     2821 taatcacaaa tcatggtcgc tattgttgca gtaattagtt gctctttggc aaccttttta
     2881 tataaaagca aaagggagtt tgtaatgaat ggatgaacag caacaattga cgaatgcata
     2941 tcattcaaat aaattatcgc atgcctattt atttgaaggt gatgatgcac aaacgatgaa
     3001 acaagttgcg attaattttg caaagcttat tttatgtcaa acagatagtc aatgtgaaac
     3061 aaaggttagt acatataatc atccagactt tatgtatata tcaacaactg agaatgcaat
     3121 taagaaagaa caagttgaac aacttgtgcg tcatatgaat caacttccta tagaaaacac
     3181 aaataaagtg tacatcattg aagactttga aaagttaact gttcaagggg aaaacagtat
     3241 cttgaaattt cttgaagaac caccggacaa tacgattgct attttattgt ctacaaaacc
     3301 tgagcaaatt ttagacacaa tccattcaag gtgtcagcat gtatatttca agcctattga
     3361 taaagaaaag tttataaata gattagttga acaagacatg tctaagccag tagctgaaat
     3421 gattagtact tatactacgc aaatagataa tgcaatggct ttaaatgaag aatttgattt
     3481 attagcatta aggaaatcag ttatacgttg gtgtgaattg ttgcttacta ataagccaat
     3541 ggcacttata ggtattattg atttattgaa acaggctaaa aataaaaaac tgcaatcttt
     3601 aactattgca gctgtgaatg gtttcttcga agatatcata catacaaagg taaatgtaga
     3661 ggataaacaa atatatagtg atttaaaaaa tgatattgat caatatgcgc aaaagttgtc
     3721 gtttaatcaa ttaattttga tgtttgatca actgacggaa gcacataaga aattgaatca
     3781 aaatgtaaat ccaacgcttg tatttgaaca aatcgtaatt aagggtgtga gttagatgcc
     3841 aaatgtaata ggtgttcagt ttcaaaaagc gggaaaatta gaatattata cacctaatga
     3901 tatacaagta gatatagatg actgggtagt tgtcgaatct aaaagaggca tagagatagg
     3961 tattgttaaa aatccattaa tggatattgc tgaagaggat gttgtgttac ctcttaaaaa
     4021 tattattcgc gttgctgatg acaaagatat tgataaattt aattgtaatg aacgagatgc
     4081 tgaaaatgca ttaatactat gtaaagacat tgtaagagaa caaggtttgg acatgcgttt
     4141 agtcaattgc gaatatacat tagataaatc gaaagttatt tttaatttta cggcggatga
     4201 tcgtattgat tttagaaaat tagtaaaaat attagcgcaa catttaaaaa cacgtatcga
     4261 gttgagacaa attggtgtaa gggatgaagc caaattgctt ggcggtatcg gaccttgtgg
     4321 taggtcgtta tgttgttcta catttttagg agattttgaa ccagtatcga ttaagatggc
     4381 taaggatcaa aatttatcat taaatccaac taaaatttct ggtgcatgtg gtcgtttgat
     4441 gtgttgttta aaatatgaaa atgactatta tgaggaagta cgtgcacaat tacctgatat
     4501 tggtgaagca attgaaacgc ctgatggtaa cgggaaagta gttgctttaa atatattaga
     4561 catttctatg caggtgaagc ttgagggaca tgaacagcca cttgaatata aattagaaga
     4621 aatagaaact atgcattaag gaggcattat tacatttgga tcgcaatgaa atatttgaaa
     4681 aaataatgcg tttagaaatg aatgtcaatc aactttcaaa ggaaacttca gaattaaagg
     4741 cacttgcagt tgaattagta gaagaaaatg tagcgcttca acttgaaaat gataatttga
     4801 aaaaggtgtt gggcaatgat gaaccaacta ctattgatac tgcgaattca aaaccagcaa
     4861 aagctgtgaa aaagccatta ccaagtaaag ataatttggc tatattgtat ggagaaggat
     4921 ttcatatttg taaaggcgaa ttatttggaa aacatcgaca tggtgaagat tgtctgttct
     4981 gtttagaagt tttaagtgat taatcaagca cactcaaata gtgttataat tataaatgaa
     5041 tatggtttgg ataagtctga gacaatgcat gtttcaggct ttaattgtgt ataaagtttt
     5101 ggtgattgca taagagatgg cggtactaaa tgttattatt aagtgtgcac gcagtatcat
     5161 tagttataaa atgtagctgt taaaagtcaa aaatacatcg aatgtagtta gtcatataat
     5221 ataaaaagag atttcaatta ctcaatagaa aaaggttgtc ttcataggag ttaaaaatgt
     5281 taaaagagaa tgaacgattt gatcaactaa tcaaagaaga ttttagtatt attcaaaatg
     5341 atgatgtttt ttcgttttca acggatgctt tgttgttagg gcattttaca aaacctagaa
     5401 caaaagatat tgtgttggac ttatgttcag gcaatggggt gatacccttg ttattgtttg
     5461 cgaaacatcc acgacatata gaaggtgttg agattcaaaa aacacttgtc gatatggcgc
     5521 gacgcacatt tcaattcaat gatgttgatg aatatataac aatgcatcac atggatttga
     5581 aaaacgttac taaagtattt aaaccttcac aatatacttt agtaacgtgt aatccacctt
     5641 attttaaaga gaatcagcaa caccaacatc aaaaagaagc acataagata gcgagacatg
     5701 agattatgtg tacacttgaa gattgcatga ttgcagcccg tcatttatta aaagaaggtg
     5761 gcaggctaaa catggtacat cgtgcagaga gactaatgga tgtcttgttt gaaatgagaa
     5821 aagtgaatat tgaacctaag aaagtcgttt ttatatatag taaagtaggg aaatcagcac
     5881 aaacgatagt agtagaaggt cgaaaaggtg gaaatcaagg tttagaaatc atgcccccat
     5941 tttatattta taatgaagat ggtaattata gcgaagaaat gaaggaagta tattatggat
     6001 agtcattttg tatatattgt aaaatgtagt gatggaagtt tatatacagg atacgctaaa
     6061 gacgttaatg cacgtgttga aaaacataac cgaggtcaag gagccaaata tacgaaagta
     6121 agacgtccgg tgcatttagt ttatcaagaa atgtatgaga caaagtctga agcattgaag
     6181 cgtgaatatg aaattaaaac ttataccaga caaaagaaat tgcgattaat taaggagcga
     6241 tagtatggct gtattatatt tagtgggcac accaattggt aatttagcag atattactta
     6301 tagagcagtt gatgtattga aacgtgttga tatgattgct tgtgaagaca ctagagtaac
     6361 tagtaagctg tgtaatcatt atgatattcc gactccatta aagtcatatc acgaacataa
     6421 caaggataag cagactgctt ttatcattga acagttagaa ttaggtcttg acgttgcgct
     6481 cgtatctgat gctggattgc ccttaattag tgatcctgga tacgaattag tagtggcagc
     6541 cagagaagct aatattaaag tagagactgt gcctggacct aatgctgggc tgacggcttt
     6601 gatggctagt ggattacctt catatgtata tacattttta ggatttttgc cacgaaaaga
     6661 gaaagaaaaa agtgctgtat tagagcaacg tatgcatgaa aatagcacat taattatata
     6721 cgaatcaccg catcgtgtga cagatacatt aaaaacaatt gcaaagatag atgcaacacg
     6781 acaagtatca ctagggcgtg aattaactaa gaagttcgaa caaattgtaa ctgatgatgt
     6841 aacacaatta caagcattga ttcagcaagg cgatgtacca ttgaaaggcg aattcgttat
     6901 cttaattgaa ggtgctaaag cgaacaatga gatatcgtgg tttgatgatt tatctatcaa
     6961 tgagcatgtt gatcattata ttcaaacttc acagatgaaa ccaaaacaag ccattaaaaa
     7021 agttgctgaa gaacgacaac ttaaaacgaa tgaagtatat aatatttatc atcaaataag
     7081 ttaatcactt tatcgattat atgaaatttt aaacgatttt ataaacgcaa gctgtaattt
     7141 taaatggtaa gttatcattt tgcattgata ctgataaaat gatgttgact atgataaaaa
     7201 aatgatgaca tcgacgtttt ttaatgtaaa ataaatacat tgaaagtaat aaatacctta
     7261 acattgaata agatgaaaat gagatgacga gataaatgtt cgcgtccgtt gaaatgcata
     7321 gaaatcttag atattatttg aagtgagaca ttacgaggag gaacagttat ggttaaagaa
     7381 acattttata taacaacccc aatatactat cctagtggga atttacatat aggacatgca
     7441 tattctacag tggctggaga tgttattgca agatataaga gaatgcaagg atatgatgtt
     7501 cgttatttga ctggaacgga tgaacacggt caaaaaattc aagaaaaagc tcaaaaagct
     7561 ggtaagacag aaattgaata tttggatgag atgattgctg gaattaaaca attgtgggct
     7621 aagcttgaaa tttcaaatga tgattttatc agaacaactg aagaacgtca taaacatgtc
     7681 gttgagcaag tgtttgaacg tttattaaag caaggtgata tctatttagg tgaatatgaa
     7741 ggttggtatt ctgttccgga tgaaacatac tatacagagt cacaattagt agacccacaa
     7801 tacgaaaacg gtaaaattat tggtggcaaa agtccagatt ctggacacga agttgaacta
     7861 gttaaagaag aaagttattt ctttaatatt agtaaatata cagaccgttt attagagttc
     7921 tatgaccaaa atccagattt tatacaacca ccatcaagaa aaaatgaaat gattaacaac
     7981 ttcattaaac caggacttgc tgatttagct gtttctcgta catcatttaa ctggggtgtc
     8041 catgttccgt ctaatccaaa acatgttgtt tatgtttgga ttgatgcgtt agttaactat
     8101 atttcagcat taggctattt atcagatgat gagtcactat ttaacaaata ctggccagca
     8161 ggtattcatt taatggctaa ggaaattgtg cgattccact caattatttg gcctatttta
     8221 ttgatggcat tagacttacc gttacctaaa aaagtctttg cacatggttg gattttgatg
     8281 aaagatggaa aaatgagtaa atctaaaggt aatgtcgtag accctaatat tttaattgat
     8341 cgctatggtt tagatgctac acgttattat ctaatgcgtg aattaccatt tggttcagat
     8401 ggcgtattta cacctgaagc atttgttgag cgtacaaatt tcgatctagc aaatgactta
     8461 ggtaacttag taaaccgtac gatttctatg gttaataagt actttgatgg cgaattacca
     8521 gcgtatcaag gtccacttca tgaattagat gaagaaatgg aagctatggc tttagaaaca
     8581 gtgaaaagct acactgaaag catggaaagt ttgcaatttt ctgtggcatt atctacggta
     8641 tggaagttta taagtagaac gaataagtat attgacgaaa caacgccttg ggtattagct
     8701 aaggacgata gccaaaaaga tatgttaggc aatgtaatgg ctcacttagt tgaaaatatt
     8761 cgttatgcag ctgtattatt acgtccattc ttaacacatg cgccgaaaga gatttttgaa
     8821 caattgaaca taaacaatcc tcaatttatg gaatttagta gtttagagca atatggtgtg
     8881 cttactgagt caattatggt tactgggcaa cctaaaccta ttttcccaag attggatagc
     8941 gaagcggaaa ttgcatatat caaagaatca atgcaaccgc ctgctactga agaggaaaaa
     9001 gaagagattc ctagcaaacc tcaaattgat attaaagact ttgataaagt tgaaattaag
     9061 gcagcaacga ttattgatgc tgaacatgtt aagaagtcag ataagctttt aaaaattcaa
     9121 gtagacttag attctgaaca aagacaaatt gtatcaggaa ttgccaaatt ctatacacca
     9181 gatgatatta ttggtaaaaa agtagcagtt gttactaacc tgaaaccagc taaattaatg
     9241 ggacaaaaat ctgaaggtat gatattatct gctgaaaaag atggtgtatt aaccttagta
     9301 agtttaccaa gtgcaattcc aaatggtgca gtgattaaat aactgtattt ttaaaaatta
     9361 ggagagataa ttatgttaat cgatacacat gtccatttaa atgatgagca atacgatgat
     9421 gatttgagtg aagtgattac acgtgctaga gaagcaggtg ttgatcgtat gtttgtagtt
     9481 ggttttaaca aatcgacaat tgaacgcgcg atgaaattaa tcgatgagta tgatttttta
     9541 tatggcatta tcggttggca tccagttgat gcaattgatt ttacagaaga acacctggaa
     9601 tggattgaat ctttggctca gcatccaaaa gtgattggta ttggtgaaat gggattagat
     9661 tatcactggg ataaatctcc tgcagatgtt caaaaggaag tttttagaaa gcaaattgct
     9721 ttagctaagc gtttgaagtt accaattatc attcataacc gtgaagcaac tcaagactgt
     9781 atcgatatct tattggagga gcatgctgaa gaggtaggcg ggattatgca tagctttagt
     9841 ggttctccag aaattgcaga tattgtaact aataagctga atttttatat ttcattagga
     9901 ggacctgtga catttaaaaa tgctaaacag cctaaagaag ttgctaagca tgtgtcaatg
     9961 gagcgtttgc tagttgaaac cgatgcaccg tatctttcgc cacatccgta tagagggaag
    10021 cgaaatgaac cggcgagagt aactttagta gctgaacaaa ttgctgaatt aaaaggctta
    10081 tcttatgaag aagtgtgcga acaaacaact aaaaatgcag agaaattgtt taatttaaat
    10141 tcataaagtt aaaagtgaga aagatcaccg ccataaatgt aaacgatgct atattcgttt
    10201 aatatcctat ggttctttct cacttttttg aattaaaata tcgtgcatgt ggaatacgtg
    10261 cgatagagat ggttagagct ttgaaattaa gaattgtagg aaggcgtttt aaatgaaaat
    10321 caatgagttt atagttgtag aaggacgaga tgatactgag cgtgttaaac gagctgttga
    10381 atgtgatacg attgaaacga atggtagtgc catcaacgaa caaactttag aagtaattag
    10441 aaatgctcaa caaagtcgag gcgttattgt attaacagat ccagatttcc caggagataa
    10501 aattagaagt acaattactg aacatgtcaa aggtgttaaa catgcgtata ttgatagaga
    10561 aaaagctaaa aataaaaaag ggaaaattgg tgttgaacat gccgacttaa ttgatattaa
    10621 agaagcgtta atgcatgtta gttcaccctt tgatgaagct tatgaatcaa ttgataaatc
    10681 tgtgctaata gagttggggt taatcgttgg gaaagatgca aggcgccgta gagaaatttt
    10741 aagtagaaaa ttgcgaatcg gccattccaa tggtaagcag ttattgaaaa agttaaatgc
    10801 atttggttat accgaagcgg atgtaaggca agctttagaa gatgaatgag gaagtgaaaa
    10861 tgttggataa taaagatatt gcaacaccat caagaacgcg agcgttgtta gataaatatg
    10921 gctttaattt taaaaaaagt ttaggacaga actttttgat agatgtgaat atcattaata
    10981 atatcattga tgcaagtgat attgatgcac aaactggggt gattgaaatt ggtccaggca
    11041 tggggtcatt gacagaacaa ttggccagac atgctaaaag agtattggca tttgaaattg
    11101 atcaacgttt aatacctgta ttaaatgata cactatcacc ttatgataat gtgacggtga
    11161 ttaatgaaga tattttaaaa gcgaatatta aagaagctgt tgaaaatcat ttacaagatt
    11221 gcgaaaaaat aatggttgtt gcaaacctgc cgtactatat tacgacgcca attttattaa
    11281 atttaatgca acaagatata ccaattgatg gctacgtggt gatgatgcaa aaagaagtgg
    11341 gagaacgctt aaatgctgaa gtaggttcaa aagcatatgg ttcgttatca attgtcgtac
    11401 aatactatac agagactagt aaagtattaa cggtacctaa atctgtattt atgccaccac
    11461 ctaatgttga ttcaatagtt gtaaaactga tgcagagaac tgaaccgtta gtaacagtag
    11521 ataacgagga agcattcttt aagttagcaa aagcagcatt tgcacaaaga agaaagacaa
    11581 ttaacaataa ctatcaaaat tattttaaag atggtaaaca acacaaagaa gtgattttac
    11641 aatggttgga acaagcaggt attgatccaa gacgtcgcgg tgaaacgcta tctattcaag
    11701 attttgctaa attgtatgaa gaaaagaaaa aattccctca attagaaaat taaatgattg
    11761 acaaagcaaa gcactattgt taaaatttaa attttgtttg acgaaaacgc tgcaaatatg
    11821 gtattatgta acttgtagcg aggtggagca atatgccaaa atcaattttg gacatcaaaa
    11881 attctattga ttgtcatgta ggaaatcgta ttgtactgaa agccaatgga ggccgtaaga
    11941 aaacaataaa acgttctgga attttaaaag aaacatatcc gtcagttttc attgttgagt
    12001 tagatcaaga caaacacaac tttgagagag tatcttatac atacactgat gtgttaactg
    12061 aaaatgttca agtttcattt gaagaggata atcatcacga atcaattgca cactaaataa
    12121 gacatataga gatgttagac gtttcttagt ataagaagta aatattatga taattatttg
    12181 agtgttgggc attatgttca atactctttt tatttacaaa atgtttaaca ctgatgtttc
    12241 gcttatagat ttttcagtaa atggataatt gtatttataa acacaaatac aagtaaatac
    12301 taagtaatta gatggagaaa attacttttt tattaaaaaa acactaaaaa acaaattaaa
    12361 atgtcaaata ttaattctct ttatgttaaa atcatcatat taagataacg aaaagagggc
    12421 ggaaaatgat atatgaaacg gcaccagcca aaattaattt tacgctcgat acacttttta
    12481 aaagaaatga tggctatcat gagattgaaa tgataatgac aacagttgat ttaaatgatc
    12541 gtttaacttt tcataaaaga aaagatcgaa agatagttgt tgagattgaa cataattatg
    12601 tgccttctaa tcataaaaat ctcgcatatc gtgcagcgca actatttatt gagcaatatc
    12661 aactaaagca aggtgtaaca atttctatcg ataaagaaat acctgtttct gctggcttag
    12721 ctggaggttc ggctgatgca gcagcaacgt taagaggatt gaatcgactt tttgatatag
    12781 gggcgagttt ggaagaattg gctctactag gcagtaaaat cgggacagat attccgtttt
    12841 gtatttataa taaaactgca ctatgtactg gaagaggaga gaaaatcgag tttttaaata
    12901 aaccaccttc agcttgggtg attcttgcta aaccaaactt aggcatatca tcaccagata
    12961 tatttaagtt gatcaattta gataagcgtt acgacgtaca tacgaaaatg tgttatgagg
    13021 ccttagaaaa tcgagattat caacaattat gtcaaagttt gtctaatcga ttagagccaa
    13081 tttctgtttc aaaacaccca caaatcgata aattaaaaaa taatatgttg aaaagtggtg
    13141 cagatggtgc gttaatgagt ggaagcggac cgactgtgta tgggctagca cgaaaagaaa
    13201 gccaagcaaa aaatatttat aatgcagtta acggttgttg taatgaagtg tacttagtta
    13261 gactattagg atagaagggt tgaaaagatg agatataaac gaagcgagag aattgttttt
    13321 atgacgcaat atttgatgaa ccatccgaat aaattgattc cattaacttt ttttgtgaaa
    13381 aaatttaaac aggcgaaatc ttcaataagt gaagatgtcc aaattataaa aaatacattc
    13441 caaaaagaaa agttaggtac agtaattact actgctggcg caagtggtgg tgttacgtat
    13501 aaaccaatga tgagtaaaga agaggcgact gaagttgtta atgaggtcat tactctatta
    13561 gaagagaaag aacgtttgtt acctggcggg tatttatttt tatcagattt ggtaggtaat
    13621 ccatcgctac taaacaaagt tggtaagtta attgccagta tttacatgga agaaaaatta
    13681 gatgctgttg ttaccattgc gacaaaaggt atttcattgg caaatgcggt tgctaatatt
    13741 ttaaatttac cagtagtagt gattagaaaa gacaacaagg tgactgaagg ttctacagtt
    13801 tcaattaatt acgtttcagg atcttcaaga aaaatagaga caatggtact ttcgaagaga
    13861 actttagcag aaaattcaaa tgttttagtt gtcgatgatt ttatgagggc tggtggctct
    13921 attaatggtg ttatgaattt aatgaatgag tttaaagccc atgtaaaagg ggtatcagta
    13981 cttgtagaat caaaagaagt taaacaaaga ttgattgaag attatacttc cttagtgaaa
    14041 ttatctgatg tagatgaata taatcaagag tttaacgtag aacctggcaa cagtttatct
    14101 aagttttcat aaaaggagtt ttagtattat gaaaatcatt aacacaacaa gattaccgga
    14161 agcacttgga ccatattcgc atgcaacagt tgtgaatggt atggtttata cttctggtca
    14221 gattccattg aatgttgatg ggaaaatcgt aagcgctgat gttcaagcac agacaaaaca
    14281 agttttagaa aatttaaagg ttgttttgga agaagcagga tctgatttga attctgttgc
    14341 gaaagcgacc attttcatta aagatatgaa tgatttccaa aaaataaatg aagtgtatgg
    14401 tcaatatttt aatgaacaca agccagcgcg tagttgtgta gaggttgcgc gtttgccaaa
    14461 agatgtgaaa gtagaaattg aattagtaag taaaattaag gaattataat tttcgattaa
    14521 tatgtttaat caagcttcta aataaaacag agagatatat actatagggg ggctcactac
    14581 atgaaagtga cagatgtaag acttagaaaa atacaaacag atggacgaat gaaagcactc
    14641 gtttccatta cattagatga agctttcgta attcatgatt tacgtgtaat tgaaggaaac
    14701 tctggcttgt tcgttgcaat gccaagtaaa cgtacaccag atggtgaatt ccgcgacatc
    14761 gcgcatccta ttaattcaga tatgagacaa gaaattcaag atgcagtgat gaaagtatat
    14821 gatgaaacag atgaagtagt accagataaa aacgctacat cagaagattc agaagaagct
    14881 taatcaattt tatatttagc gatgtaatac atttgcaata agttgatttg atactgtcga
    14941 taaagcataa agctttgtcg gcagtttttt tagtttgtat taatgttttt ttatttttaa
    15001 tgaaaggcta ataaatatat acgttaacag attatgatga tatgaaaatt attgattcat
    15061 tgctgataaa aattgcgttt gaatgatgct cgtatttttg aagtaagaaa aaagttgttt
    15121 ttaaaattac aacgaattaa aaacaatgcc ttttatatgt tgaaagagta ttgcagatta
    15181 aattataata atgacgaagt gtaaaattta atgggggtta atgttcatgc gaagacacgc
    15241 gataattttg gcagcaggta aaggcacaag aatgaaatct aaaaagtata aagtgctaca
    15301 cgaggttgct gggaaaccta tggtcgaaca tgtattggaa agtgtgaaag gctctggtgt
    15361 cgatcaagtt gtaaccatcg taggacatgg tgctgaaagt gtaaaaggac atttaggcga
    15421 gcgttcttta tacagttttc aagaggaaca actcggtact gcgcatgcag tgcaaatggc
    15481 gaaatcacac ttagaagaca aggaaggtac gacgatcgtt gtatgtggtg acacaccgct
    15541 catcacaaag gaaacattag aaacattgat tgcgcatcac gaggatgcta atgctcaagc
    15601 aactgtatta tctgcatcga ttcaacaacc atatggatac ggaagaatcg ttcgaaatgc
    15661 gtcaggtcgt ttagaacgca tagttgaaga gaaagatgca acgcaagctg aaaaggatat
    15721 taatgaaatt agttcaggta tttttgcgtt taataataaa acgttgtttg aaaaattaac
    15781 acaagtgaaa aatgataatg cgcaaggtga atattacctc cctgatgtat tgtcgttaat
    15841 tttaaatgat ggcggcatcg tagaagtcta tcgtaccaat gatgttgaag aaatcatggg
    15901 tgtaaatgat cgtgtaatgc ttagtcaggc tgagaaggcg atgcaacgtc gtacgaatca
    15961 ttatcacatg ctaaatggtg tgacaatcat cgatcctgac agcacttata ttggtccaga
    16021 cgttacaatt ggtagtgata cagtcattga accaggcgta cgaattaatg gtcgtacaga
    16081 aattggcgaa gatgttgtta ttggtcagta ctctgaaatt aacaatagta cgattgaaaa
    16141 tggtgcatgt attcaacagt ctgttgttaa tgatgctagc gtaggagcga atactaaggt
    16201 cggaccgttt gcgcaattga gaccaggcgc gcaattaggt gcagatgtta aggttggaaa
    16261 ttttgtagaa attaaaaaag cagatcttaa agatggtgcc aaggtttcac atttaagtta
    16321 tattggcgat gctgtaattg gtgaacgtac taatattggt tgcggaacga ttacagttaa
    16381 ctatgatggt gaaaataaat ttaaaactat cgtcggcaaa gattcatttg taggttgcaa
    16441 tgttaattta gtagcacctg taacaattgg tgatgatgta ttggtggcag ctggttccac
    16501 aatcacagat gacgtaccaa atgacagttt agctgtggca agagcaagac aaacaacaaa
    16561 agaaggatat aggaaataat catttacgta tttaaaatgg ctaggataaa aggataatcc
    16621 tatgtaatat taatgtaatc tttatgattt aatgattcgc atagtaatgg agtt
//
LOCUS       DAHHYJ010000054        16605 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000054 DAHHYJ010000000
VERSION     DAHHYJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.054"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            329..997
                     /gene="queC"
                     /locus_tag="NBK22_001286"
     CDS             329..997
                     /gene="queC"
                     /locus_tag="NBK22_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCD2414479.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            999..1418
                     /gene="queD"
                     /locus_tag="NBK22_001287"
     CDS             999..1418
                     /gene="queD"
                     /locus_tag="NBK22_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCD2414480.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1422..2135
                     /gene="queE"
                     /locus_tag="NBK22_001288"
     CDS             1422..2135
                     /gene="queE"
                     /locus_tag="NBK22_001288"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCD2414481.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPDIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            2234..2818
                     /locus_tag="NBK22_001289"
     CDS             2234..2818
                     /locus_tag="NBK22_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414482.1"
                     /translation="MILYILINIAIVVLITGFNLYRHQMQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNSLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            3098..3538
                     /locus_tag="NBK22_001290"
     CDS             3098..3538
                     /locus_tag="NBK22_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCD2414483.1"
                     /translation="MNTKYFLAVGAVASVLTLGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            3881..4354
                     /locus_tag="NBK22_001291"
     CDS             3881..4354
                     /locus_tag="NBK22_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD2414484.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIVVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            4329..5015
                     /locus_tag="NBK22_001292"
     CDS             4329..5015
                     /locus_tag="NBK22_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2414485.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            5015..6070
                     /gene="saeS"
                     /locus_tag="NBK22_001293"
     CDS             5015..6070
                     /gene="saeS"
                     /locus_tag="NBK22_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCD2414486.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTMTVTLHKLDITS"
     gene            complement(6142..7125)
                     /gene="csbB"
                     /locus_tag="NBK22_001294"
     CDS             complement(6142..7125)
                     /gene="csbB"
                     /locus_tag="NBK22_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCD2414487.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSFISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENDEKDTHKVYSK"
     gene            complement(7257..8096)
                     /locus_tag="NBK22_001295"
     CDS             complement(7257..8096)
                     /locus_tag="NBK22_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD2414488.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTDYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCNRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKNPDDVKIG
                     DLK"
     gene            complement(8318..9667)
                     /locus_tag="NBK22_001296"
     CDS             complement(8318..9667)
                     /locus_tag="NBK22_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD2414489.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE"
     gene            complement(9887..11068)
                     /gene="nagA"
                     /locus_tag="NBK22_001297"
     CDS             complement(9887..11068)
                     /gene="nagA"
                     /locus_tag="NBK22_001297"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCD2414490.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEGDDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDKALTNIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGAAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(11376..13334)
                     /locus_tag="NBK22_001298"
     CDS             complement(11376..13334)
                     /locus_tag="NBK22_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD2414491.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAASEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDEADDEVTKEEEAE
                     AEAQQVATAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGQRKGNSNDSQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GVDSFKPKSSEYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFAAGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALLVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(13340..14260)
                     /gene="pfkB"
                     /locus_tag="NBK22_001299"
     CDS             complement(13340..14260)
                     /gene="pfkB"
                     /locus_tag="NBK22_001299"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCD2414492.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDTDVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(14257..15021)
                     /locus_tag="NBK22_001300"
     CDS             complement(14257..15021)
                     /locus_tag="NBK22_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD2414493.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTVATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTIITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(15271..15753)
                     /gene="ybaK"
                     /locus_tag="NBK22_001301"
     CDS             complement(15271..15753)
                     /gene="ybaK"
                     /locus_tag="NBK22_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCD2414494.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYEVHSFEVPEEHLSGQEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLITITKGKIGAVIHE
                     "
     gene            complement(15927..16379)
                     /locus_tag="NBK22_001302"
     CDS             complement(15927..16379)
                     /locus_tag="NBK22_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414495.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
BASE COUNT         5498 a         3000 c         2357 g         5750 t
ORIGIN      
        1 ttttattttt ataatatgta ttggatgtag ctaaaacttt aaaagcattg tcattatcct
       61 gaccttgatt ttaactaata tatagtatat tctattcatc gtacataaat gaatatcaga
      121 ggttcctagc tgaaaccctc tataaaaaac tagacattga aatttcaaac attcgttggt
      181 aaacctttaa tatatattat aggagcaacc catttctttc gtctatcttt tatggagata
      241 ggcttttttt attttataaa ttaaaaacgg gcttatcaat gccattaacc tctttattaa
      301 tattattaat gataggagca attacaaaat ggaaagtgta ttaaataatg aaaaagccat
      361 cgttgtcttt agtggtggtc aagacagtac aacatgtctc ttttatgcaa aaaaacattt
      421 caaagaagtt gaactcgtaa cgtttaatta tggccaaaga catgatactg aaattgaagt
      481 cgcaaaacaa attgcacaag atcaaggaat gaaacatcac gttttagata tgtcattatt
      541 atcacaactt actccaaacg cattaacaca acatgatatg gaaattacta ataatgaaga
      601 tggtatacct aatacatttg ttccagctag aaatttactt ttcttgtcgt ttgcaggcgc
      661 tctagcttat caaattgggg ctaagcatat tattacaggc gtatgtgaaa cagacttttc
      721 aggctaccca gactgtcgcg atagttttat aaaatcaatg aacgtaacat taagcctagc
      781 tatggacaaa gattttgtca ttcatactcc tttaatgtgg ttaaacaaag cagaaacgtg
      841 gaaattaagt gatgaactcg aagttttaga ttatattcgt acaaaaacat taacatgcta
      901 taacggtatc attggggatg gctgtggtga atgtccagct tgtcatttac gtcaacgtgg
      961 actaaatcaa tatcttgaaa gtaaaggagc gctttaaaat gttacaacaa atctatccta
     1021 gtacaacgca tccatatcaa ttcgaattaa ataaagattt taatttttcg gctgcacatc
     1081 acattccttg tgaagaagca ggtatttgtc aaaatgtcca tggtcatact tactttgtta
     1141 atttaacaat tgtcggtgat aaactagatg acactggctt cttagtgaac tttagccatt
     1201 tgaaaaagat gatacacggt aaatttgacc atcaactgtt aaataactta cctgctttta
     1261 aaaacaaaat cccttcaact gaaatcgtag cggaaacaat ttatcaaatt gttaaagaaa
     1321 atttggcatc gctcgaacac caaccaaaat gtattcaagt atttgtaaga gaaacaccaa
     1381 caagttatgt tgtatttaga ccaaaggaac aggtgtaact aatggctaaa attcctgtac
     1441 tcgaaatatt cggtccaacc attcagggtg aaggtcgagt aatcggtaga aaaacgatgt
     1501 ttgtgagaac agctggatgt gattatcgct gtagctggtg tgattctgca tttacttggg
     1561 atggtagtgc taaaggcgat ataaaactca tgaccgctga agaaatttat gatgaattaa
     1621 aacgaattgg tggcgattta tttaatcacg ttacaatttc tggtggtaac ccagcattaa
     1681 ttaaaggtat ccaagagtta gttgacttat ttcaagataa aggtattttc agcgcactgg
     1741 agacacaagg cagtaaattc caaccttgga tgacacaaat tgatgattta acgattagtc
     1801 caaaaccacc aagttcaact atgacacctg atttaaaaaa gttagatgaa gtgattacac
     1861 aatgcgtacc ttcctcatta aacttaaaag tagttgtatt cgacgataaa gattatgatt
     1921 ttgccaaaat gatacaccac cgttatccag atattccctt ctatttacaa gttggtaacc
     1981 catatttatc agacagcgta gataatcata ccgaaaagtt gttagaacgc tacgagcagt
     2041 tggtggattt agttatgcaa agtaatgata tgaatcacgt ttatgtgtta ccacaacttc
     2101 atacattact ttggagtaat aaaaaaggtg tataaaggat aacttatttt actttttata
     2161 agtcatatct aatgaataac aatatattaa ttgatataat agttgattga agtataacga
     2221 acaggagtcc atcatgattt tatatatatt aattaatatt gctatcgtcg tacttataac
     2281 tggattcaat ttatataggc atcaaatgca acatttatca ttaagtgcaa tgttgttatc
     2341 tattacgata aacgccttta taaatacatt cataattgat aaatataact ttattactct
     2401 gtgtactata acgatgttta tcatttggac gatactacag ttttacattg ataaaaaatt
     2461 aaaacctgta tacattacag accaaaaatt tattgctatc atattaacga ttgttgtcag
     2521 tttaacgcaa cgtgttaccg acttttcaag tacacaatca atatatatgt ctattccatt
     2581 tttagcccct gcaattttca tcattggtgg tattatgttg tttatttcaa catttaatag
     2641 cttagatgaa actgctgaaa ataataacaa aataaagaaa ctaatgatta aaggacttat
     2701 cattattaac atttcattta tcgttatgat ggttttaaca ccatattggt acttgtattt
     2761 aatcgtctat cttattttct tgttgttttt attgtggcaa aaggtttata aattttaata
     2821 ccaaaactat aaaacacttc tgatattctt agttcaaaat atcagaagcg ttttatagtg
     2881 ttatctagtt cagataaata ttttctcacc taaaaaaatg ccctcctctt attttgactc
     2941 ctatttattt aaatcagaca attattttca ttttcaaatt attctttctt caatattagt
     3001 taagcgatat ttaaacgaag ttaagaatta gttaatggca tattatttgc cttcatttta
     3061 aacttaactt atcaaattga agaaatgagg agttagcatg aatacaaaat attttttagc
     3121 agttggtgct gttgcctctg tattaacttt aggcgcttgt ggtaattcta attcacaaga
     3181 tcaaggtaac aaaactgaac aaaaaacaaa gtcagaagat agcaatgtta aaactgataa
     3241 aacaaaacac ctaacaggta cattcagttc taaaaacggt gaaactgttg aaggtaaagc
     3301 tgagattaaa aatggtaaat taatgcttac taactacaaa tcatcaaaag gtccagattt
     3361 atacgtctac ctaacaaaaa atggcgacat taaaaacggt aaagaaatcg caatggttga
     3421 ctacgataaa gaaaaacaaa catttgatct taaaaatgta gatttaagca aatatgatga
     3481 agtaacaatc tattgtaaaa aagctcacgt catcttcggc ggcgctaaat taaaataatc
     3541 aatttctgag ttaaactttt atttacaaca tactattact atacattaca aattttaaaa
     3601 atatacatta cactcattac tcaatggaaa gcgtatgatt tcacagcccc cctagcttgt
     3661 agaaatcata ctttcctttt ttcaatatat atacaactat taaatcccat aagattgtag
     3721 agcacataag taaaattttt ttagagcttg aggtttgttt agcttaagca acccatgagc
     3781 tcaaacactt cctgttcaca taacactaca aatcgcatta tgttgcttaa tcttatgttt
     3841 atataaatta cacacaataa atagaaagaa tgtgaacatc atgaataaat tattgctact
     3901 cgttacattt atcattcgtg tgggttcagg tattgttatg ttaatgcaag gctacgaaaa
     3961 attaacgggc ggatttacgc tgaaaggttt agtaccagtc atcgctaaca atactgattc
     4021 accagagtgg tataagtggt ttttcgcaaa tatagttgca catacgacgt cattatttga
     4081 tattgttgtc ccactcggag agattgcaat tggattaggt ttaatttttg gagtttttgc
     4141 atatgctgct agtttctttg gagcctttgt tatgataaat tatatcttag cagatatgat
     4201 atttacgtat cctcttcaat taactttctt tatcctttta ctaatgagtc actcattgtt
     4261 aaaacagatt tcacttaaag aaatcattaa ttactttaga ggtcgtaaga acagaggtga
     4321 aaaaatagat gacccactta ctgatcgtgg atgatgaaca agacattgta gacatttgtc
     4381 aaacctattt tgaatatgaa ggttacaaag taacaacgac aactagcggt aaagaagcaa
     4441 tttctttact atcaaatgat attgatatca tggtacttga tatcatgatg ccagaagtta
     4501 atggttacga cattgtcaaa gaaatgaaaa ggcaaaaatt agatatcccc tttatctatt
     4561 taactgccaa aacacaagaa catgatacca tttacgcctt aactttaggt gcagatgact
     4621 atgtcaaaaa accatttagt ccaagggaac tcgttttacg tattaataat ttacttacaa
     4681 gaatgaagaa ataccatcat caaccagttg aacaactgtc gtttgatgaa ttaacactta
     4741 ttaacttaag taaagttgtg actgtaaatg gtcacgaagt ccctatgcgt attaaggaat
     4801 ttgagttatt gtggtattta gcttctagag aaaatgaagt tatttctaaa tcagaattac
     4861 ttgaaaaagt ttggggatat gactattacg aagatgctaa taccgtgaat gtccatatac
     4921 accgtattag agaaaaatta gaaaaagaga gctttacaac atataccatc acaactgtat
     4981 ggggattagg atataaattt gaaaggagcc gataatggtg ttatcaatta gaagtcaaat
     5041 cattattggc gtcgtatcga gtatactatt aacttcaact attttagcaa ttgcatatat
     5101 tttaatgtgg tttaacggcc atatgacact aactttgacc ttaacgacaa taattacaag
     5161 ctgtttaacc ttattaatat gtagtatttt tattaatcca cttatacaaa aaattaagca
     5221 gtttaatata aaaactaagc aatttgctaa cggaaattac gcaagcaatg ataaaacgtt
     5281 taattcacca aaagaaattt atgaattaaa tcaatctttt aataaaatgg cttctgaaat
     5341 tacgcaacaa atgaatcaaa ttaaatccga acaacaagaa aaaacagaac tgattcaaaa
     5401 cttagcccat gatttaaaaa cacctttagc aagcattatt tcatattctg aaggactacg
     5461 tgatggtata atcacaaagg atcatgagat taaagagtca tacgacatat taattaaaca
     5521 agcaaacaga ttatcaacat tatttgatga tatgactcat attatcactt taaatacagg
     5581 taaaacatat cccccagaat taatacaact agaccaatta cttgtatcaa tattgcaacc
     5641 atatgagcaa cgtatcaaac atgaaaaccg cacattagaa gtgaatttct gtagcgaaat
     5701 tgatgcattt tatcaatatc gaacgccact tgagcgtatt ttaacaaact tacttgataa
     5761 tgcgctaaaa ttttcaaatg ttggtagtcg cattgatatt aatattagtg aaaacaaaga
     5821 tcaagatact atcgacattg ctattagcga tgaaggtatt ggcattatac cagaactaca
     5881 agaacgtata ttcgaacgta cattcagagt agaaaactct cgtaatacaa aaacgggtgg
     5941 ttctggatta ggcttatata tagctaatga actcgcgcaa caaaataacg caaaaatcag
     6001 tgtaagcagt gatatagatg taggaactac gatgactgta acattacaca aattagacat
     6061 tacgtcataa tccgatttat ttataaaata aaatgcaaag actaaaaaga agctcccatt
     6121 aatgagggct tctttttttg tttatttaga ataaacttta tgggtatcct tctcatcatt
     6181 ttcaatactt gaaagatttg tagcttgaat aatatattta ggacgtgcct taacttcata
     6241 atatatcctg ccaatatatt cacctacaac accaattgaa attaactgta tgccgcctaa
     6301 taataaaata gctgcaatcg ttgaaaaata tcccggaata ttaacaccag atatcataat
     6361 attgatgaat aaatagataa tatatagcac gcttattgaa aagataaaca agccaagata
     6421 aatcatcatt ctcaaaggtt tactattaaa ggaaatcaat ccatcaatac cataattaaa
     6481 taacttttta aaggaccact tagattcccc tttttgtctc tcaacatttt gatacgtaaa
     6541 cactttagta ttatagccta tccattcaaa taaccctttt gaaaatcgat tatattcttc
     6601 aagtgatgca atggatttaa cagctctttg gcttaaaagt ctaaaatcac caacaccatc
     6661 atcaaattgt acttcttcta caaagcaatt aactaactta taatacaaat ggcttaatgt
     6721 tttgcgacta aaattttcac cacttctatc acgctttgca atcacttgat catagccttc
     6781 cataaaacct tcaaccattt tcggaatata ttcaggtgga tgttgcaaat cacaatctat
     6841 catgatgact gcatcaaatt cagtactatg ctggtaacct gcaatcatag ctgcttcttt
     6901 tccaaaattt ctactaaaag aaataaagct gacatgccta tctattgtgg caagatgttg
     6961 catttcatct atagtggtat ccgtactacc atcatctatg aaaagcatat tataatcata
     7021 gcctttcaca ctactatctt gtgaaagtat ttcagttaat tgttgatgtg tttgtgtaat
     7081 gacttcccct tcattaaaac aaggaatgac gactctaatt ttcatctatg tccatccttt
     7141 ttatttaaaa gattttgaat gtacctgagt attgttttat caaatagtaa aaaccaactc
     7201 atttatttta atgtacacat taaaaggcat tcataaacgt aaaatttaac atccagttat
     7261 tttaaatccc cgattttcac atcatctgga tttttacctt gtcttgcgtt tctatttaaa
     7321 gcatcaattt cagctacttc tgttaattct aaattaaaat ctaaaatatc tatattttct
     7381 tgaatgcgtt taggtgtctg agattttgga ataataattc tattgtgtgc taaatgccaa
     7441 cgtaatacaa cttgtgctgg tgttttatga tatttttcag caattttaac aatgacaggg
     7501 tcgtctagta gtcctctatt tctcatcaaa ggcatccatg cagtcacttt aatatcgtga
     7561 cgattacaaa attcttgtaa ttcttgttgg ttaaaatatg gatgtacctc aatttgattc
     7621 accattggtt tgatacttga ttgagccatt aatttttcta gatgatgaac attaaaatta
     7681 catacaccta ttgcttttac cttaccttgc tcgtaaagtt cttccatagc tttatatgtt
     7741 tctaaaaata gaccatctgc ttcacaaggc caatgtatta gaaataaatc aagataatca
     7801 gtttgtaaat tttcaatcga tttgttgaag tattcgaatg ttttctcata accttgatag
     7861 tcattccata acttcgttgt tataaacaaa tcttctctat cgacgccatt atcctttaat
     7921 gctcgtccta gtgaagcctc attatcataa aagtatgctg tatcaaacgc tctatagcct
     7981 gcgtcaattg cagcatttac aactttagtc atatcttcgt cagagatttt ataaacacct
     8041 aatccaactg aaggcatcgg gtatccatta tttaatattt gtatctcatt caacatcttt
     8101 atatctctcc aatctatgta tcttttatat ctttacatta ccctaaattt ttcaacaaac
     8161 tcaattaata cgaattatcg ctttcaataa aaaatattca ttaaatcatt aaagatattg
     8221 agttccaata ctattttcac ttttcattaa ttttagtcaa aaaaataacc aaccaaaaat
     8281 gaattaaatc attcttagtt ggtttatata ttaatatcta ttctgatttt tcatcttcat
     8341 cagactgtcc gatagtaggt ctcgcttcat taaattcata gtttaatatc acccaaataa
     8401 tttggtggtt atcgatttct gaaacaaccc agcgatcata agttgtatcc acgtaatcat
     8461 ctttttgtaa attggtatta cgagattgta accatccacc tatcgtatca atatcctcag
     8521 agtcatcaaa ttctataccg aactcttcag ttaaatcatc caatagtact ctaccattta
     8581 cttggaatgt cttattatca attttaacga tatcattcac ttcatcatca tcaaattcat
     8641 cacgaatttc tccaacgatt tcttctaaaa tatcttccat cgttaaaata cctgccgttc
     8701 caccatattc atctataata agactcatat gtacatgttc acgttgcatt ctaattaatg
     8761 catcactgat acgtgttgtc tctgaaatca ttggcaactc atgtatatag tttgctattt
     8821 taatcgtttt tccagaagcg tattcagtta aaaattcttt gacgttaata aatcctttaa
     8881 tgtggtcttt atcaccatca tcagtaattg gataacgcgt aaattgatgt tcttttattg
     8941 tttctagtaa ttcgtctaca ttaaaaggtt catttagtgt aatcatttga gttctaggta
     9001 ccattatatc ttttgcatgt ctttcatcga atgaaaagat attttgcata tatgccaatt
     9061 cagtttggtt gatttctcca ccattataac tattgttaat aataattttg atttcttctt
     9121 ctgacattgc atcagtttgg gcatcaggat ttacaccaaa cattctaata ataacacgtg
     9181 cagaaccatt catcagccaa atcaatggtt tcataatgtt accgaaatag aacaatggtc
     9241 ttgcatatac taaagcaagc ttttcagtat gttgaatcgc tatagattta ggcgctaatt
     9301 caccaagtac tacatgcaaa tacgtaacga ttataaatga cactgcaaac gaaatcgtcg
     9361 tcgttaatgc agttggtaaa ttgattgctt caaatatcgg gtgtaatagc ttttcaaatg
     9421 ttggttcacc aagccaacct aaccctaaag atgttactgt tatacctaac tgacaagcag
     9481 aaagataata atctagatta gcaatcatct tttttactat tttagcaggt ttatttcctt
     9541 catctgctag ctgttcaatt cttgttgctc taatttttac taatgcaaat tctgaaccaa
     9601 caaatacagt ggttaatgca attaatagaa taaatataat caaactaatt atggtcgaag
     9661 tttccaatta attccctatt tctagggttt cacctccatg ttatatgtgt cactcatggg
     9721 taacacgcat tcaaatttat cactatgact taaattacaa ctacatgtta tcgccttccc
     9781 atttagacac ccccagaaaa aatgttattg cttctatttt atcataatat aaagtgctta
     9841 tgttaacaga ttaaatctat tgcatacatt taattatgat tatttattag ctaaatgtgt
     9901 gaaccttacc ttgttttatt gtagatttta cattcatatc atcatctaga ataacaagat
     9961 ctgcatcctt atttacttta atactacctt ttctatcatc gatacctaat gcaatggctt
    10021 gatttaaact tgttactcgc cataaatgat ctaatgtatc acctgtaaat gatattaagt
    10081 tacgtaaccc atgattcatt tttaaaatac taccagcaag cgcaccattt gcaagacgtg
    10141 cttgttgcga ttgaacagtt actttttgtc cacccaaatc atattctcct tcaggcatac
    10201 cttttgcacg cattgcatcg gtaattaaat aaaaacgttc attacctttc atacggtaag
    10261 caatagcaac cgatgccgga tgagaatgtg tgccatcaac aatcatttcg gtatgtagag
    10321 catcattcaa ccatgctgct ccaaaaacac ctggttctct atgctggaat ggcgtcgctg
    10381 cattatataa atgcgtgaca tgtttagctc ctcgctcaac agcttcgact gcttcttcgt
    10441 atgttgccac tgtatgacca attgaaaaaa taatgtcatc tttatacgtt tcaagcgctt
    10501 cttttgcacc ttcaacttca ggtgcaaacg tcataatctt tattaatccg ttagcagtct
    10561 cttgaaaatg tttaatttta tcgataaatg ggcgtacaac gtattgcgga tgttgagcac
    10621 caactttatt ttcagatata aatggtcctt ctaaatgtat acctacaatt tccgctgcat
    10681 tgtgaacatc ttgctccgct tcatatttag caatatttgt aagtgcttta tctattttat
    10741 cagtagattg cgtcattgta gtggccaagt atgatgtcgt cccttcagat aacaaatttt
    10801 cggatagata ttttaagcca tcgtatgacc catccattgc atcttgacca taaccaccat
    10861 gaatatgtat atcaataaaa cctggtaata catgatgacc tttagcatca atcacttgaa
    10921 ttttatttgt cgtatcatta tcaattgctg ctttatcatc cccttctcca attgcaacaa
    10981 tctgtccatc tttcacatga atgtaaccat tatcgatttt gccatcttca gtataaactt
    11041 tgccgttata tataattaat tctgacacgt ataaaccctt ctttatttat aaattttcac
    11101 tttaagacac tcatatttta ctatatctgc ttcgtttggc ataacttttt gcaaaatatt
    11161 aaaaaagtct atacatttgt ggtgtctatt tcttcattca tttatctaaa aataaaaaca
    11221 ccttcatatc aattcataaa aactgaatta atagaaagtg tttttacagc tttgtataag
    11281 ctaccgttaa cacgatgtat aaaactaaca tatattcatt gaacttacaa cttaatatga
    11341 agctctttgc taacaatcat tttacatcat taaaactact cgtccattga ttttgaagct
    11401 tcgatttctg tttcagttaa cttgggtttg attaaaccgt aaattaatgc tgaaactaat
    11461 gtaccaacta gaagtgcaat aagagtttga agtaagtgtg caccatcagt accaacaatt
    11521 acaataatac caccatgtgg cgcagtaatt cgagaaccta agcctaaagc aattgcgcca
    11581 cctatacctg aaccaatcat cattgaagga ataacacgta atggatcggc agctgcaaat
    11641 ggaatcgcac cttctgtaat aaaggacata cccatcacat agttagggat aattgaacca
    11701 cgttgttctt ttgtaaattt acgtctgaaa attaacatcg ctgtcgcaat tgctaacggt
    11761 ggaatcatac caccaatcat tgcagctgta attggtgctg cattaccttc aatcaacgca
    11821 cctgttgcaa aaacatatgc cgctttgttg aatggaccgc ccatatcaat cgccatcata
    11881 gcgccaataa ctaaacctaa taatacaata ttagaacctg ataaattgtt taatccatct
    11941 aataacaaat gatttaacca agatgctggt ggattaaaga cataaatcat caataagcct
    12001 gtagctgtca ctgtcaatag tgggtaaatt aatgttggtt ttaatccctc taatgcttgt
    12061 ggtaacttac gtgtcatcgc tttaacacct tgtgttaagt aacctgctaa gaaacctgca
    12121 ataataccac caataaatcc tgaaccacct gaaattgcta acataccacc tacaagacca
    12181 gctgcaaaac caggtttatc cgcaatacta cgtgcaatga atccagataa aattggaatg
    12241 attaacgcga atgcactttt actaccaatg ttccatagct gctctgcaaa cgcattgtac
    12301 tctgagcttt ttggtttaaa tgagtctact ccaaataaaa atacgattgc cattaaaata
    12361 ccaccagata ttacaagtgg caacatgtta gaaacaccgt tcattaagtg tttatagaaa
    12421 gctttacctg ggcttaattt ttcttgactg tcatttgagt taccttttct ttgaccatca
    12481 cgggcaacaa aaggtttacg acttgtatct aatgctttat taattaattc ttctgggcgt
    12541 ttaataccat ctgctactgg tacttctacg acatttttac catcgaagcg atccgtctca
    12601 acatgaacat cagcagcaac aatgatacct gttgcatttt caatatcttg ttcagttaaa
    12661 tggtttttaa tgccgcttga accgttcgtt tcaactttaa ttttaatacc cattttatcc
    12721 gcttgctttt tcaatgcatc acgtgccata tatgtgtgtg caatacctgt tggacaagca
    12781 gttactgcta acacatatgg ctcattagat tgtttagatg attgttctgc agttgcaact
    12841 tgttgtgctt cagcttctgc ctcttcttct tttgtcactt catcatcagc ctcatctata
    12901 atcgctagta cttcttcagg tgatgaagca tgtaataatt tctcacgtac attttcatcc
    12961 attaaaatac cagacaactt agctaaagca tctagatgtg tttgggcgcc accttctgac
    13021 gctgcaatca taaagaataa gtgtgctggt tgcatatcca aactttgata atctacgcct
    13081 gctttagatt taccaaacgc aatagctggt gacttaactg cggccacttt ggcatgtgga
    13141 atggcaatac cttcgccgat accagttgta ctttgtgatt ctcgattgtg aatcgcttcc
    13201 ttaaatgacg cgacatcact taatttacct gctttgtcta attgatttac taactcatca
    13261 ataacaccat ttttgtcatt tgccattaaa tccattgcta ttgtatcttt tgttaataac
    13321 tctgttactc tcattatttt cactccccat caagtacgct aatcgtaact tgtgatttta
    13381 ttttttctat agcgtcccgt gttgctaagt cctcatcaaa tgccgtggca gtaccgcatg
    13441 cgactgcttg ttggaatgct ttttcaatcg ttaaacctga agcaattcca gccaccatgc
    13501 ctgcaactgt actatcacca gagccaactg tattaaccac tttcccttgt ggattaactg
    13561 ctttaatact gatttcttta tcaatataaa tagcaccatc accgccaagc gagacaataa
    13621 cagattgcgc acctttatca actaacaaac gaccatattt aataacatct gtgtctgagt
    13681 tcactgttgt attaaacatc acttctaatt catctttatt aggtttaata aatagtggat
    13741 gatatggtaa aacgctttca gccaattctt tttcagcgtc gactactaat ttagcacctg
    13801 tctgtgctgt aatttgtgca atttgcgcat acgcatcgct tggaatacta cttggtacac
    13861 ttccagcaac aataactata tcttcgcttg ttgtattttt aatttgttgt aacagttgtt
    13921 caaattgtgt tgacgttata tgaggacccg gtgcattgat ttctgtttct tgtcctgttt
    13981 ttaatttcac attaatacgt gtatcttcat caacttcaat aaaattcgat tgaattgcac
    14041 tgttatttaa tgtatctata atgaatttcc caggaaatcc acctgcaaat cccaaggcag
    14101 ttgactcaac atccaatgtc tttaagacgc gcgagacatt aatacctttc cccccagcga
    14161 atttatatgt tgctgttgct ctgttcaaac catcaatttt aaaatcattc gtaaaaatga
    14221 catagtcaat tgaaggattg aaagtcactg tataaatcat aaagtccctc ctataaagtg
    14281 atacttttgt tggtattctt ttaacgattc ttgatttaat gctttttcag atgtgatgat
    14341 tgtcgtactt tctagcaaag gtacacgagc aaaatatact ttattaaact tagaatgatc
    14401 tataagtaca aatgattgat tggctaatga cattgctgtt tgtttaacta atgcctcttg
    14461 ctcatcggga gtagttaatc caagttcaat atctaatcca ttcatcccga taaaagcttt
    14521 atcgaaacaa tatcgtctta atatctccat agcactagaa ccaatcgtag caactgtatt
    14581 ttctttaact tgaccaccta gcataattgt tttaatacct tttttaagta aagcttctac
    14641 atgtgttaaa ccattggtta ccacaatgat atctttcgct tgaatatatt taattagctc
    14701 caatgtagat gaaccagcat cgataaataa gcattcatta tcgttgattt gattagctgc
    14761 tattttagca atcattttct tttcatcaag attcgttgct aatttttcag ttaaattcgc
    14821 ctcaaccata cgattttctt ttaacattgc accaccatgc acacgttgca atttccctaa
    14881 ttgttgtagt ttagataaat ctcttcgtat tgttgaagca ctgcatccag ttcgatctat
    14941 tagttcttgt aaagtcaaaa aatctttgtg cgaaagttct tctaatatta actcgtgtct
    15001 tttttctgta attatcatca tcaccccatc tcactctgta ttataatgag tacgctttca
    15061 cttttcaatc ataaattacc aaaaacaatc acaaacaatc aaattgtgac gataaaatta
    15121 catttttatg ttcaaatact catttgtaat gactgtttat gatcgtaaaa tgctaattca
    15181 atcaaaaagg agtgtggcag aaatgaattt tataaaaatt tcatctctgt cacactccga
    15241 atcttgcccc atactttttg ttgttattaa tcattcatgg ataactgctc caattttgcc
    15301 tttagttatt gtaatcaaat cctcaacagc tattgtgatt tgcattgttc gaagcccacc
    15361 actcacactg atatgactat aattttcaca cgatttgtca acgactgttg gaaacaatgt
    15421 tttcatacca acaggcgaac acccaccacg aatgtatccc gttacatttt tcaaattatc
    15481 taaaggcata agctgcaatt tcttctctcc aaccaaagca gctgcctttt tcatatctaa
    15541 agtttcactt actgggataa caaatacaaa atgttcatgt tttgtatttt ctagaacaag
    15601 cgttttaaat actgttttaa catttgcttg tatgagttct gcgacttctt gaccagataa
    15661 atgttcttct ggtacctcaa agctatgaac ttcgtattta atttttgcac gatcaagcat
    15721 acgcatcgcg ttcgttttct tatttttagc catttcaaca cctcatttat acttcttcag
    15781 aactatatta acattttaaa atacattacg gattggttga ttttgacata acatttacat
    15841 cattttataa gttgaattca cctttttaaa ttaaattatt gcaaaataaa aacagtatat
    15901 ccaattagaa tacactgttc catctattat ttcgttatta tgccttgatt tgaaatactt
    15961 acatgttgct tactagttga attatcattg gaatacacat aattatatcc atgatttgtc
    16021 ttagaaattc tttctaaacc ataattatca ttgatatgct tatctgcata acgatcagca
    16081 ataacttggc actcttgttc gttaaaagaa tttgatttat ttgcattcac acgatgttta
    16141 aaatgatttt gctttttcac attttcattc acttcagatg ctttagcagt atgaatattg
    16201 agaatatcca ttttcttttt ataagtatct tcagttgata catttatcgc gttaagcgta
    16261 ttaattgttt tatgtacctt agcaagcgca tcttcatttg ttgctaatat taataaacca
    16321 attaaacaaa cggttaatat tacagcaaca aagcctaaaa ataaattttt tactgtcatc
    16381 cccttaccca catttcctta ctcatttttt atcatcttaa cacattaacg ttaatgaatt
    16441 aggtatgtgg attgcaattt aaaaagaatg taatatttgt aacatgttta ttactcaaaa
    16501 tcattttttg ttacatataa catttaaata taaagagtaa aacatttagt caatcgaatc
    16561 tttttaaatg taaagcatcg aattttctta ataaaattca taaga
//
LOCUS       DAHHYJ010000055        15931 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000055 DAHHYJ010000000
VERSION     DAHHYJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.055"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(297..1049)
                     /gene="sarS"
                     /locus_tag="NBK22_001303"
     CDS             complement(297..1049)
                     /gene="sarS"
                     /locus_tag="NBK22_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCD2414496.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
     gene            complement(1418..2416)
                     /gene="sirC"
                     /locus_tag="NBK22_001304"
     CDS             complement(1418..2416)
                     /gene="sirC"
                     /locus_tag="NBK22_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCD2414497.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSIDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(2413..3408)
                     /gene="sirB"
                     /locus_tag="NBK22_001305"
     CDS             complement(2413..3408)
                     /gene="sirB"
                     /locus_tag="NBK22_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCD2414498.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTILVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIIFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(3424..4416)
                     /gene="sirA"
                     /locus_tag="NBK22_001306"
     CDS             complement(3424..4416)
                     /gene="sirA"
                     /locus_tag="NBK22_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCD2414499.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            4647..5627
                     /gene="sbnA"
                     /locus_tag="NBK22_001307"
     CDS             4647..5627
                     /gene="sbnA"
                     /locus_tag="NBK22_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCD2414500.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            5624..6634
                     /gene="sbnB"
                     /locus_tag="NBK22_001308"
     CDS             5624..6634
                     /gene="sbnB"
                     /locus_tag="NBK22_001308"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCD2414501.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            6655..8409
                     /gene="sbnC"
                     /locus_tag="NBK22_001309"
     CDS             6655..8409
                     /gene="sbnC"
                     /locus_tag="NBK22_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCD2414502.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIAGIVSNSEITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRASKQPLTAAEFWQTIANVNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EVDYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTAPIKEQATDMLNDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVMVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANDRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            8402..9658
                     /gene="sbnD"
                     /locus_tag="NBK22_001310"
     CDS             8402..9658
                     /gene="sbnD"
                     /locus_tag="NBK22_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCD2414503.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTAP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSVLLICIAVITFIVCIFGALKLIETTHMPKSQTPNTNKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYIFATVACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVIGQIIGSLSGAAITSYTTPATTFIVMGIVFAVSSLFL
                     ICSTITNQINDHTLMKLWELKQKSAK"
     gene            9648..11384
                     /gene="sbnE"
                     /locus_tag="NBK22_001311"
     CDS             9648..11384
                     /gene="sbnE"
                     /locus_tag="NBK22_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCD2414504.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSD
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQHMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYAEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHQDVLLTRYVEGKEDQVEKVLYQLADIDISEIPKDFI
                     LLPIHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDLVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSPMSKLSQVIEQSGLAPE
                     AWLECYLNRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            11293..13143
                     /gene="sbnF"
                     /locus_tag="NBK22_001312"
     CDS             11293..13143
                     /gene="sbnF"
                     /locus_tag="NBK22_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCD2414505.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVN
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQCLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEQRQWQWVKGIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            13118..13894
                     /gene="sbnG"
                     /locus_tag="NBK22_001313"
     CDS             13118..13894
                     /gene="sbnG"
                     /locus_tag="NBK22_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCD2414506.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEVVNAHGKHFCALPREDE
                     NIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDDG"
     gene            13894..15096
                     /gene="sbnH"
                     /locus_tag="NBK22_001314"
     CDS             13894..15096
                     /gene="sbnH"
                     /locus_tag="NBK22_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCD2414507.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPKIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKSWSEKHRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVENFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVQIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            15100..15864
                     /gene="sbnI"
                     /locus_tag="NBK22_001315"
     CDS             15100..15864
                     /gene="sbnI"
                     /locus_tag="NBK22_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCD2414508.1"
                     /translation="MNHIHEHLKLVPVDKIDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
BASE COUNT         5319 a         2551 c         3110 g         4951 t
ORIGIN      
        1 ttccgaaaga tttatttagg aagtaaaagg cttaaaaccg caaaatcacg ctatttcgat
       61 taataaaaac agataaaata tagagatatt ttttataatg aaagcggttt aatacatatg
      121 agttaaaaaa tatttttaag aataaagtgg ggctttgaat gtgctgaggt ttaatttcgg
      181 aaatggtttt aagtgatata gaaaatagaa agttgttcat tatgtgctga ggttttatta
      241 aaaaatagaa aacacaagtg cactttagaa tacagcacac ttgcgtaata gagatattat
      301 tcaaaaacaa gatgtaaatg atctttatca gctaataatt gattcacttg agctaataat
      361 tgttcagcat ggtcttgctg cgcgtcatcc atatgaatta aaatttttct ttcatcttca
      421 gttgagcgtt cttttattag atagccttgc ttttttaaat tattgagagc tctaacagtt
      481 tgagggtatt tatggtggat tgtttcaatt aaatctttaa gaagaacgat gtttttattt
      541 tgagaagtga taatagctag aattgtgaat tctacaaaac ttaatgttag atgttttttg
      601 ataatattct tgaaatacat tgtatacatc atcaagttta gaaattcttt actatctttt
      661 ggtatcatct gtgattcact ttgatctgca aggttaaatt gtttaatgat ttgatcaaac
      721 aatgtaacac gttctgcaat tttctctcgt tgttcttcag atattgaaat gtaagtatta
      781 cgctcatcaa ttttacttcg aactttacta atatatgaat gtttcacaag tacttttata
      841 tgctgtacta aatccgattg tttataacat aaatctgaaa caatcttctt aaatggaagt
      901 gtgttttctt gctgatgaaa taaataagtc agtaatataa attcttttat agtcatatcg
      961 acttcaggct tgactttttt cttaaaacga aacatatatg cttcaatgat tataaaatct
     1021 ctaattttgt catggttatt atatttcatt gttttatctc cttgtatatg cactttattt
     1081 taatataaca caatataata agaaaaataa tccataattt acaattaaaa ataatatgat
     1141 atttttatta aaatttgtgt ttttgaataa atgacattat caaacactat atttaaataa
     1201 gaaatagtta tcgatatttg aaaaatcgaa gaatattgat tgatattata gaatatgagt
     1261 cggtgttatt tgtatataaa gtattaatat gtaattaaga aagtcgacgc ttgttaacaa
     1321 taacgacaac tatatgtata aaaaaacctc gcaactgtta gttaactgaa tcatttagct
     1381 aacttcgttg tgaggtcatt ttgttttaat aatatcgtta taactttttc acggttaata
     1441 ataagtatat gaagaatggg gcaccaaaag cagcaataaa tacacctgct ggcacttctt
     1501 taggcaagaa taaggtacgc ccaattaagt ctgcaataac aattgatatg gcaccaatca
     1561 ttgctgacat tagtaacttt ttagcataac ttccgcgaac gattgttttc gcgatatgtg
     1621 gtgcgattaa accgacaaac ccaatgttac ctactaaact gattgccata gatacgagta
     1681 tagtagaagt gattaattgg attagtttca tacgttgtac atgtaagcct aagccaatcg
     1741 ctacagggtc atcaagtata gatattttca tttttggtat aacaagaaat aacaacggca
     1801 caacagctaa aataaccata cccaaaatga ttgtatcttt aaacgtagca ccgtaaagac
     1861 ttccgactag ccatgtataa gctttggcag cagataattg cttcgttgta atgagtaatc
     1921 cttggacaag cgcaataaac aacgtttgca tcgaaatacc gatgattatg agtgttgtcg
     1981 ggcgtatttg tcctttcgtt tgaaacacta atagtatcat cattgcaact gcgccaccta
     2041 atactgcaaa tagtggaagt aaatgtatcg ttaaatggct gaaaaatgca ataaagacaa
     2101 cagcacttaa gctagcacca cctgtgattc cgataatatc tggtgaggca attggatttt
     2161 tcaatacatt ttgtaacatt aaaccactca tccctagtgc ggcaccagct aatatcgcaa
     2221 gtgtaatgcg aggtaagcgt aatacttcta aagtgaattg atctatacta tcatttggat
     2281 ttataaagta catcagtacg cgttgtaatg gtataaagct tgaaccaatc atcatactta
     2341 ccactgaaac gatggctaaa aagattaacg cgaagatgag atggtaattg tcttttttat
     2401 taatcttttc ggtcataagc gttgacgtcc tttcttcata atatagatta agacaatagc
     2461 gccaatgaca gcggtaacga caccgatagg caactctaat ggcttaatta ttatacgagc
     2521 aacaatgtct gaaatgatca ttaggattgc tccagctaat gcagtaaaag gaattaaata
     2581 cttatagttt ggtggtaata atcgtttgct aatattcggt acgataagac ccacaaagat
     2641 gattgatcca gctacggcta ccgaaatacc ggctaacata ctgatgagca taataatcat
     2701 ccatttgatt aattttatgt tttgaccgag gccggttgca atgtcgtcac ttgtcatcaa
     2761 gatgttgatg tgtgcagcca tgctaaatgc aattaaaata agtatcaata caagcggaat
     2821 aatccatggg atatcccaaa tattacgtaa tgaaacggag ccacttaacc aaaataatag
     2881 gccttgtaag tctgtttcgt tcataataag tatgccttga gtaaaggctg taaatagcat
     2941 cgcaatcgca gcacctgcca aaatgacacg gtgaggtgag aatagtgttt gtctaaacat
     3001 acctagcgca acaactaata tagtaacaac aatggcacct aaaaatgcaa taactacaat
     3061 cattttaaaa gattgaattt ggataaatgt aatactaaaa atgacaaaaa atactgcgcc
     3121 tgcattgaca ccgaaaagcc ctggtgaggc tattgggttt cgtgtaagtg cttgcatcaa
     3181 caaacctgag acagcaaggg cagcaccagt caataacgca atgattgttc tcgacgcccg
     3241 tgcaccagtg acaacatcat gtaaatcgtt ttcactatca aagttgaata acgcctgtat
     3301 caccgtacct ggtgacacaa gcgtatttcc aatcattaaa cttaagatag ctactattgc
     3361 aagacataaa ccagcaataa cgatttggta ttttggttta agtagcatcg taaaactcct
     3421 taattatttt gattgttttt caatatttaa cttttcatat aaatcgtcaa taagttttaa
     3481 tgaagattta tatccgccag ctaagttcca agtgatttca tctaaatcat cagatacttg
     3541 gttgttttta actgcgtcta aatttttcca ctctttactt gaagtccatt cgctttcagt
     3601 ctttttaact aatgcagcat ctttcgcatt tggatctgat tttactacaa aaatatgatc
     3661 agcgttcatt aatggaatgc tttctttaga tgtaagttgg ataatatctt taccattatc
     3721 aacttgtttt tgtaagtctt tattacgttt gaatcctaaa tcatttaaga tttcaccagc
     3781 atatccacca gcataaattc ttgtatgatc agcacggaag ttaacaactg aagctttcaa
     3841 tggccatgca tctttatact ttgcttttgc atctttttgg aatgcagcta ctttatcatc
     3901 gtacttttta agtaaatctt cagcttcttt ttctttccct aaagctttcc ccattaactt
     3961 agttgtatct ttgaatttga aaactgtatc agtagaaact gttggtgcaa ttttagataa
     4021 ttgatcgtaa actttttcgt ttctaacttt tgacgcgaca attaagtccg gttttaattt
     4081 agagatttcc tctaagttag gtgcaggttc ttgacctaca atcttagtat cttttaaatc
     4141 attttttatg tattcgaatt tcggtttttg tgtccatgat tctacagcac ctacaggttt
     4201 aacacctaaa gatacagcga cgtcagtggc accttgatat agcgtaacaa cacgctttgg
     4261 tttcccttta atttcagttg tacccattgc atgtttaatt gaagttgttt ccttatcttt
     4321 gttatcagat gattgtttat ttgaattccc actacatcct gctaaaacaa gtaggaaagc
     4381 aagcgtaaca acaagcattt taattacttt attcattgac taattagcct ccttcgtgat
     4441 gtatgacaat gagaatcatt atcacgatta agtatgaatt aaattttttc ctaagtcaat
     4501 aaaatattta tgatttacat gcaacttata attatttgac atataaattc ataaaaaata
     4561 taatcctaat tacttgatag tgagaatcat tatcaattag gtaacacaca atattataga
     4621 attttaaatt tgaggaggaa gcgcttttga ttgaaaaaag tcaagcatgt cacgattcat
     4681 tgttagattc tgtagggcaa acacctatgg ttcaacttca tcaactattt ccgaaacatg
     4741 aagtgtttgc aaagttagag tatatgaatc ctggaggcag catgaaagat cgacctgcca
     4801 agtacatcat tgaacatggt attaaacatg gtttaatcac tgagaataca catttaattg
     4861 aaagtacttc tggtaattta ggcattgcgt tggcaatgat agctaaaatc aagggattaa
     4921 aactcacgtg tgttgttgat cctaaaatat caccaacaaa tttgaaaatt attaaaagtt
     4981 atggtgccaa tgtagaaatg gttgaagaac ctgatgcaca tgggggttat ttaatgactc
     5041 gtattgcaaa ggtgcaagaa ctgttagcca ctattgacga tgcatattgg attaatcaat
     5101 atgcgaatga gttaaattgg caatcccatt atcatggtgc aggcacagag attgttgaaa
     5161 caattaagca acctatagat tattttgtcg cgccagtcag cacgacaggt agcattatgg
     5221 gtatgagtag aaaaataaaa gaagtgcatc caaacgcaca aattgttgct gttgatgcga
     5281 aagggtcagt catttttggt gacaaaccta ttaatagaga attacctggt atcggtgcta
     5341 gtcgtgtacc cgaaatattg aatagatcag aaattaatca agtgatccat gtagatgatt
     5401 atcaatctgc tttgggctgt cgaaaactga ttgattatga aggcatattt gccggaggtt
     5461 caacaggttc gattattgca gcgattgagc agttgataac gtcaattgaa gaaggtgcaa
     5521 caattgtcac gattttacca gatcgaggcg atcgttactt agatttagtt tattcagata
     5581 catggttaga aaaaatgaaa tcaagacaag gagttaaatc agaatgaata gagagatgtt
     5641 gtatttaaat agatcagata ttgaacaagc gggaggtaat cattcacaag tttatgtgga
     5701 cgcattaaca gaagcattaa cagcccatgc gcacaatgat tttgtacaac cgcttaagcc
     5761 gtatttaaga caggatcctg aaaatggaca catcgcagat cgaattattg caatgccaag
     5821 tcatatcggt ggtgaacacg caatttcagg tattaagtgg ataggtagta agcacgacaa
     5881 tccatcgaaa cgtaatatgg agcgtgcaag tggcgtcatt attttgaatg atccagaaac
     5941 gaattatcca attgcagtta tggaagcaag tttaattagt agtatgcgta ctgcagcagt
     6001 ttcagtgatt gcagcaaagc atttggctaa aaaaggattt aaagacttaa caatcattgg
     6061 atgcgggcta atcggagaca agcaattaca aagtatgtta gagcaattcg atcatattga
     6121 acgcgtgttt gtttacgatc aattctctga agcatgtgca cgctttgttg atagatggca
     6181 acaacagcgt ccggaaatta attttattgc gacagaaaat gctaaagaag cagtatcaaa
     6241 tggtgaagta gtcattacat gtaccgtaac ggatcaacca tacattgaat atgattggtt
     6301 acaaaagggt gcatttatta gcaacatttc tatcatggat gtacataaag aagtctttat
     6361 taaagctgac aaagtcgtag tagatgactg gtcacaatgt aatcgagaaa agaaaactat
     6421 taaccaattg gtgttagaag gtaaattcag caaagaagca cttcatgctg aactagggca
     6481 acttgtgaca ggtgacatac caggacgtga agacgatgat gaaatcatat tacttaatcc
     6541 gatgggtatg gctatcgaag atatttcaag tgcttacttt atttatcaac aggcacaaca
     6601 acaaaatatt gggacaacat tgaacctata ttaagaatgc gaggtgtctg aacattgcag
     6661 aatcatacag cagtcaatac agcacaaacg ataatattaa gggatttagt tgatgcatta
     6721 ttatttgaag atatagccgg aattgtatcg aatagtgaga ttactaaaga aaatggacaa
     6781 acgcttttga tatacgaacg tgaaacacaa caaataaaga tacctgttta ttttagtgct
     6841 ttaaatatgt ttcgttatga aagttcacaa ccaattacga tagagggaag ggcgtctaag
     6901 caacctttaa cggcagctga attttggcaa acaattgcta atgtgaattg tgatttaagt
     6961 catgaatggg aagtggctcg cgttgaagaa ggactgacta ctgctgccac acagcttgct
     7021 aaacaattat cagaattaga tttagcgtca catccttttg tgatgtcaga gcagtttgca
     7081 agtttaaaag atcgtccatt tcatccatta gctaaagaaa aaagaggatt aagagaagtg
     7141 gattatcaag tgtatcaagc tgaattaaat caatcatttc ctttaatggt tgcagcagtt
     7201 aaaaagacac atatgattca tggcgatact gcaaatatcg atgaattaga aaatttgaca
     7261 gcacctataa aagaacaagc gacagacatg ttaaatgatc aagggttatc aatagatgac
     7321 tatgtactat ttccggtaca tccttggcaa tatcagcata ttttgccgaa cgtctttgcg
     7381 aaagagatta gtgaaaagtt ggttgtacta ttaccgttaa aatttggaga ttatctgtcg
     7441 tcttcaagta tgcgttcatt aattgatatt ggcgcaccgt ataaccatgt caaagtacca
     7501 tttgcaatgc agtcattagg cgcattaagg ctaacgccta cgcgttacat gaaaaacgga
     7561 gaacaagcag aacaattatt acgtcagctt atagaaaaag atgaagcact agctaagtat
     7621 gtcatggttt gtgatgaaac agcttggtgg tcatatatgg gtcaagataa tgatattttc
     7681 aaagatcaat taggtcatct aactgttcag ctaagaaaat atcccgaagt gctagccaaa
     7741 aatgatacgc aacagctagt gtcaatggca gcactcgcgg caaatgatcg cactttatat
     7801 caaatgattt gtggaaaaga taatatttct aaaaatgatg tcatgacgtt atttgaagat
     7861 atcgcgcaag tctttttaaa ggtaacacta tcatttatgc aatacggcgc attaccagag
     7921 ttgcatggtc aaaatatatt gttgtcattt gaagatggac gtgtacaaaa atgcgtgcta
     7981 cgtgatcatg atactgtcag aatttataaa ccatggctaa cagcacatca gctttcattg
     8041 ccgaagtatg tcgtcagaga agatacacct aatacgctaa ttaatgaaga tttggaaaca
     8101 ttctttgctt attttcaaac attagctgta tcggtaaatc tatatgccat tattgatgca
     8161 attcaagatt tatttggtgt aagtgagcat gaacttatgt cgttgttaaa acaaatttta
     8221 aaaaatgaag tggcaactat ttcctgggtt acaactgatc agctagctgt cagacacatt
     8281 ttatttgata aacagacgtg gccattcaaa caaattttat taccattgct atatcaacgt
     8341 gatagtggtg gaggtagtat gccttcaggt ttaactaccg taccaaatcc aatggtgaca
     8401 tatgattaat cagtctatat ggcgcagtaa ctttcgcatt ttatggctca gtcagtttat
     8461 agcgattgct ggactgacag tacttgtgcc attattgcca atttatatgg catcactaca
     8521 aaatctatca gtagtagaaa tacagttgtg gagtggtata gcgattgctg ctccagctgt
     8581 aacgacgatg atagcttcac cgatatgggg gaagctaggt gataagatta gtcgaaaatg
     8641 gatggtgtta agagcgttac ttggtttggc ggtatgctta tttttaatgg cattgtgtac
     8701 ggcaccatta cagtttgtac ttgtgaggtt attgcaggga ctatttggtg gtgttgttga
     8761 tgcatcaagt gcgtttgcga gtgcagaggc gccagctgaa gatcgtggaa aggtattagg
     8821 aagactgcaa agttcagtca gcgcagggtc tcttgtggga ccattaattg gcggtgttac
     8881 agcttcgata ttaggtttta gtgtgttact gatttgtatt gccgttatta cttttattgt
     8941 ctgtattttc ggtgcattaa aattgattga aacgacacat atgccaaaat cacaaacacc
     9001 aaatactaat aaaggtattc gccgttcatt tcaatgtcta ttatgcacac aacaaacatg
     9061 tcgatttatt atcgttggcg ttttagcaaa ctttgctatg tatggcatgc taactgcatt
     9121 atcaccactt gcttcatcag tgaatcatac agcgatagat gaccgtagtg tgattggatt
     9181 tttacagtcc gcattttgga cggcttcaat attaagcgcg cctttatggg gtcgctttaa
     9241 tgataaatca tatgttaaat cagtatatat atttgccacg gttgcatgtg gttgtagtgc
     9301 gatactgcaa ggtttagcga cgaatataga gtttttaatg gctgcaagaa tacttcaagg
     9361 attaacatat agtgcattga ttcaaagtgt catgtttgtt gttgtaaatg cttgtcatca
     9421 gcaacttaaa ggcacatttg ttggaacgac aaacagtatg ttagttattg gtcaaattat
     9481 tggtagtctt agtggcgctg ccattacaag ttatactaca ccagctacta cgtttatcgt
     9541 tatgggcata gtatttgctg taagtagttt atttttaatt tgttcaacca tcactaatca
     9601 aatcaacgat cacacattaa tgaaattatg ggagttgaaa caaaaaagtg caaaataaag
     9661 aattaataca acatgcagcg tatgcggcta tcgaacgcat tttaaatgaa tattttagag
     9721 aagaaaattt atatcaagtg ccacctcaaa atcatcaatg gtctatacaa ttgtcagatc
     9781 tcgaaacttt aacgggtgaa tttcgctatt ggtctgcgat ggggcatcat atgtatcatc
     9841 cagaagtatg gcttattgat ggaaaaagta aaaaaataac aacttataaa gaagcaattg
     9901 cacgtatttt gcaacatatg gctcaaagtg cagataatca aacggcagtg caacaacata
     9961 tggcgcaaat tatgtctgac atcgataata gcattcatcg cacggcgcgc tatttgcaaa
    10021 gtaacacaat agactacgca gaggatcgct atatcgtttc agaacaatct ttatacttag
    10081 gtcatccatt tcatccgact cctaagagtg caagtgggtt ttcagaagca gatttagaga
    10141 aatatgcacc cgaatgtcat acatcattcc aattacatta tttagctgtg catcaagatg
    10201 ttctgctcac gcgctatgta gaaggtaaag aagatcaggt tgagaaagta ttgtatcaat
    10261 tagcagatat agacatatca gagataccca aagattttat tttattacca atacatcctt
    10321 atcaaatcaa tgtgttgcga cagcatccac agtatatgca atatagtgaa caaggtttaa
    10381 taaaagacct tggcgtttcc ggtgatttag tgtacccgac gtcttcggtt agaactgtat
    10441 tttcaaaagc attaaacatt tatttaaaat taccgataca cgttaaaatt actaatttta
    10501 tacgtacgaa tgatcttgaa caaattgaac gaacaattga tgccgcgcaa gttatcgcat
    10561 cagtcaaaga tgaggttgaa acaccccatt ttaaattgat gtttgaagaa ggatatcgtg
    10621 cattgttacc gaatccatta gggcaaacag ttgaacctga aatggattta ttaacaaata
    10681 gtgccatgat tgttcgtgaa gggataccga attaccatgc tgataaagat attcatgtat
    10741 tggcgtcatt atttgaaacg atgcctgatt caccgatgtc taagttatca caagtgattg
    10801 agcaaagtgg tttagcacca gaagcatggc ttgaatgtta tttgaatcgt acattattgc
    10861 cgatattaaa gctgtttagt aacacaggca ttagtctaga agcacatgta caaaatacat
    10921 taattgaatt aaaagatggc atacccgacg tatgctttgt cagagatctt gaaggcattt
    10981 gtctatctag aacgattgct actgaaaaac agcttgtgcc aaatgttgtg gcagcatcaa
    11041 gccctgttgt atatgcacat gatgaagcat ggcatcgtct taaatattac gttgtagtaa
    11101 atcacttagg acatttagta tcaactattg gtaaagcgac tagaaatgaa gttgtgttat
    11161 ggcaacttgt agcgcatcgt cttatgactt ggaaaaaaga atacgcgaat aacgcagtat
    11221 ttgttgactg tgtagaagat ttatatcaaa cgccgaccat tgcggctaaa gcgaatttga
    11281 tgagtaaatt gaatgattgt ggtgcaaacc ctatttatac acatatacca aatccaattt
    11341 gtcataacaa ggaggtatcg tattgtgaat caaacaattc ttaatcgtgt aaagactaga
    11401 gtgatgcacc aactggtatc atcacttatt tatgagaata ttgttgtgta taaagcgtca
    11461 tatcaagacg gtgtcggtca ttttacaata gaaggacatg attcagagta tcgttttact
    11521 gctgaaaaga cacatagctt tgatcgtata cgtatcacat caccaattga gcgtgtcgta
    11581 ggagatgagg cagatacaac aacagactat acacaattat tgagagaggt tgtatttaca
    11641 tttcctaaaa atgatgaaaa gctagaacaa tttattgtcg agttattaca gacagaatta
    11701 aaagatacgc aaagtatgca gtatcgagaa tcaaacccac cagcaacacc tgagacattt
    11761 aacgactatg aattttatgc gatggaaggg catcagtatc atccaagtta caaatcacgt
    11821 ttaggattta cgttgagtga taatttgaaa tttggtcctg attttgtacc aaacgttaaa
    11881 ctgcagtggt tagctatcga caaagataaa gtagaaacga cggtatcaag aaatgttgta
    11941 gttaacgaaa tgttacgtca acaagttggc gataagactt atgaacattt tgtacagcaa
    12001 attgaagcgt ctggcaaaca tgtaaatgat gttgagatga tacctgtaca cccatggcag
    12061 tttgaacatg tcatccaagt tgatttggct gaagaaaggc ttaatggcac agtactatgg
    12121 ttaggggaaa gtgatgagct atatcaccct caacaatcga ttcgtacgat gtcgccaata
    12181 gacacgacaa aatattattt aaaggtacca ataagtataa cgaatacttc aacgaaacga
    12241 gtgttggcgc ctcatacaat tgaaaatgca gcgcaaatta cggattggtt aaagcagata
    12301 cagcaacaag atatgtattt aaaagatgaa ttaaagacag tttttctagg ggaagtctta
    12361 ggacagtctt atttaaatac acaactttcg ccttataaac aaactcaagt ttatggtgcg
    12421 ttaggtgtta tatggcgtga aaatatatat catatgttaa tcgatgaaga ggatgcgata
    12481 ccatttaatg cactttatgc aagtgataag gatggtgtac cattcattga aaattggatt
    12541 aaacaatatg gttctgaagc ttggacaaag caatgtttag ctgtagcgat tcgtccaatg
    12601 attcatatgc tttattatca cggtattgcc tttgaatcgc atgcacaaaa tatgatgctc
    12661 attcatgaaa atggttggcc tacacgtatt gccttaaaag atttccatga tggtgttcgt
    12721 tttaagcgtg agcatttaag tgaagctgct tcacacctga cattaaagcc aatgccagaa
    12781 gcacataaaa aagtgaatag taattcattt attgaaacag atgacgaacg tttagtacgc
    12841 gactttttac atgatgcatt tttctttatt aatatcgccg aaatcatctt atttattgaa
    12901 aagcaatatg gtatcgatga gcagcgacaa tggcaatggg ttaaaggcat catcgaggcg
    12961 tatcaagaag catttccaga gttgaataac tatcaacatt tcgatttgtt tgaacctacg
    13021 attcaagttg aaaagttaac gacacgtcga ttattaagtg actccgagtt aagaattcat
    13081 catgttacaa atccattagg tgtaggaggt atcaatgatg caacaactat ctctgaaaca
    13141 tagattaaac aatggtgatt cagtttatgg catttttaat tctataccgg acccattgat
    13201 gatcgaggtt atcgcagcaa gcgggtatga ctttgttgtg attgatacag aacacgtggc
    13261 gattaatgat gagacactag cgcatttaat tcgtgcagct gaagcagcgc atattatacc
    13321 aattgtacgt gtcactgcag tgatagatag agatatcatt aaagtgttag atatgggtgc
    13381 gagaggtatt attgtgccac acgttaaaga tcgtgagaca gttgagcata ttgtgaaatt
    13441 aagtcgttat tacccgcaag gattaagaag tttgaatggt ggtcgcatgg caagatttgg
    13501 acgtacacca ttacttgatg caatggagat ggctaatgag catattatgg tgattgccat
    13561 gatagaagat gttgaagggg ttatggccat tgacgatata gctcaagtcg aaggtttaga
    13621 catgatagtc gaaggtgccg cagatttatc gcagtcactt ggcataccat ggcaaacgcg
    13681 tgatgatcaa gtaacatcac atgttcaaca tatttttgag gttgtgaatg cacatggtaa
    13741 acatttttgt gcattaccac gtgaagatga aaatattgca aaatggcagg cacaaggtgt
    13801 acaaacattt attttaggtg atgatcgcgg aaaaatatat cgccatttaa gtgcatctct
    13861 agcgacgtct aaacagaaag gggatgatgg ctaatgcgta tagttcaacc tgttattgaa
    13921 caattaaaag cacaatctca tccagtttgt cattatatct atgatttagt cggactggaa
    13981 catcatttgc aacatattac atcgtcattg ccgagtaatt gtcaaatgta ctatgcaatg
    14041 aaagcaaata gtgaacgaac aatcctagat acaattagtc agtatgttga aggattcgaa
    14101 gttgcatctc aaggtgaaat agcaaaaggt cttgctttta aaccagcaaa tcatattatt
    14161 tttggtggcc ctggtaagac agacgaggaa ctaagatatg cagtaagtga aggtgttcag
    14221 cgtattcatg ttgaaagtat gcatgaatta caacggctaa atgccatctt agaagatgaa
    14281 gataagacac aacacatttt attgcgtgtt aatttagcag gaccatttcc caatgcaacg
    14341 ttgcatatgg caggacgccc aacacaattt ggtatttctg aagacgaagt tgatgatgtc
    14401 attgaagctg cgctagcaat gccaaagatt catctagatg gctttcattt tcattctatt
    14461 tctaacaatt tagactcgaa tttacacgtc gatgtagtga aactttattt taaaaaagca
    14521 aaatcatggt ctgaaaaaca tcgatttcca ctcaaacata tcaatcttgg tggtggtata
    14581 ggggttaact atgcagattt aactagccaa tttgagtggg ataattttgt agaaaatttt
    14641 aaaacactta tcgttgagca agaaatggaa gatgtgacat tgaactttga atgtgggcgc
    14701 tttattgtgg cacatattgg ttactatgtg acagaagtgc tagatattaa gaaagtgcat
    14761 ggtgcttggt atgccatttt aagaggaggt acgcaacaat ttagactgcc ggtatcttgg
    14821 cagcataacc atccttttga aatttatcgt tataaggaca atccatattc atttgaaaaa
    14881 gtttcaattt cgagacagga cacaacgtta gtcggtcaat tatgtacacc gaaagatgtc
    14941 tttgctagag aagtgcagat agacgcaatc agtacaggcg acgttattgt tttcaaatat
    15001 gcaggtgcat acggatggtc tatttcacat cacgatttct taagccatcc acatcctgaa
    15061 tttatttatt taacgcaaac aaaggaggat gaataactat tgaatcatat tcatgaacat
    15121 ttaaaattgg taccagtaga taagattgat cttcacgaaa cattcgaacc tttaagattg
    15181 gaaaaaacga aaagtagtat tgaagcagat gattttatac gtcatcctat tttagtgaca
    15241 gcgatgcaac atggtagata tatggttata gatggtgtgc atcggtatac aagtttgaaa
    15301 gcgttaggat gtaagaaagt tccagtgcaa gaaatccatg aaacacaata ttcaattagt
    15361 acatggcaac ataaagttcc atttggtgtg tggtgggaaa cgttacaaca agaacatcgc
    15421 ttgccatgga ctactgagac aagacaagaa gcgccattta ttacaatgtg tcatggtgat
    15481 acagaacaat atttgtatac aaaagattta ggcgaagcac attttcaagt atgggaaaag
    15541 gttgtcgcaa gttatagtgg ttgttgttct gtagagagaa ttgcacaagg tacatatcct
    15601 tgtctttctc aacaagatgt actcatgaag tatcagccat tgagttataa ggaaattgaa
    15661 gcggttgttc ataaagggga aactgtgcca gcaggtgtga cacgctttaa tatttcagga
    15721 cgatgtctta atcttcaagt accactggca ttacttaaac aagatgatga tgttgaacaa
    15781 ctgcgcaatt ggaagcagtt tttagcagat aagtttgcca atatgagatg ctatactgaa
    15841 aaagtatact tggtggagca atagttttac tgtgatgttg agggaaatat gatgatttag
    15901 cgtattgata gcgaaaatat aataaaacaa t
//
LOCUS       DAHHYJ010000056        15849 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000056 DAHHYJ010000000
VERSION     DAHHYJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.056"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..165)
                     /locus_tag="NBK22_001316"
     CDS             complement(<1..165)
                     /locus_tag="NBK22_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414509.1"
                     /translation="MAQRYIKVLILYIFTTLLSSITVTSKCVPNKVVRFILRTVVGYG
                     IFAYGLHYFSN"
     gene            complement(252..719)
                     /locus_tag="NBK22_001317"
     CDS             complement(252..719)
                     /locus_tag="NBK22_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCD2414510.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVINMGVEQGMNQTLDQLDALLK"
     gene            complement(905..2452)
                     /gene="adcA"
                     /locus_tag="NBK22_001318"
     CDS             complement(905..2452)
                     /gene="adcA"
                     /locus_tag="NBK22_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCD2414511.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHGGYDPHVWLDPKI
                     NQTFAKEIKDELVKKDPKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISHE
                     SIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTETI
                     RKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAESK
                     HDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKDL
                     KAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFKL
                     VDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDMEVLLKEMDNWPTYYPSKLNKGQI
                     KEEMLAH"
     gene            complement(2641..2712)
                     /locus_tag="NBK22_001319"
                     /pseudo
     CDS             complement(2641..2712)
                     /locus_tag="NBK22_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2747..3013)
                     /locus_tag="NBK22_001320"
     CDS             complement(2747..3013)
                     /locus_tag="NBK22_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD2414512.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLESIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIKIASCKYHY"
     gene            complement(3013..3264)
                     /locus_tag="NBK22_001321"
     CDS             complement(3013..3264)
                     /locus_tag="NBK22_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD2414513.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(3429..3530)
                     /locus_tag="NBK22_001322"
                     /pseudo
     CDS             complement(3429..3530)
                     /locus_tag="NBK22_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3537..4136)
                     /locus_tag="NBK22_001323"
     CDS             complement(3537..4136)
                     /locus_tag="NBK22_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCD2414514.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(4155..4517)
                     /locus_tag="NBK22_001324"
     CDS             complement(4155..4517)
                     /locus_tag="NBK22_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCD2414515.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            4772..6022
                     /gene="fmhA"
                     /locus_tag="NBK22_001325"
     CDS             4772..6022
                     /gene="fmhA"
                     /locus_tag="NBK22_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCD2414516.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNEEVVAACLLTEARTLKFFKYFYTHRGPVMDYTNQSLVSFFFKSLTSYLKKQNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDTLGYKHQGFSVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYDDHAMLKLAYIDLNEYLKTLQLKQQQLTAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLNSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPLLYKVQTYLNHKRR"
     gene            complement(6119..6850)
                     /locus_tag="NBK22_001326"
     CDS             complement(6119..6850)
                     /locus_tag="NBK22_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD2414517.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVEQGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVYVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKDEARGKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAKEVSNNIVFIHEGIIGEQ
                     GAPEEMFNRPKTEELRRFLNVINEE"
     gene            complement(6847..7566)
                     /locus_tag="NBK22_001327"
     CDS             complement(6847..7566)
                     /locus_tag="NBK22_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD2414518.1"
                     /translation="MFLNLNSEQQHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKILRSISRVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(7547..8326)
                     /locus_tag="NBK22_001328"
     CDS             complement(7547..8326)
                     /locus_tag="NBK22_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCD2414519.1"
                     /translation="MKRLLFVMIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSRSAFAF
                     SKKADDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(8447..9886)
                     /gene="mdeA"
                     /locus_tag="NBK22_001329"
     CDS             complement(8447..9886)
                     /gene="mdeA"
                     /locus_tag="NBK22_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCD2414520.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDVFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVVITTAYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIDMMNQIKVDTMLHGYHAG
                     FLFALLITVVSFFCSFMLQGKKKEVDSRQ"
     gene            10060..10164
                     /locus_tag="NBK22_001330"
     CDS             10060..10164
                     /locus_tag="NBK22_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCD2414521.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(10318..11004)
                     /locus_tag="NBK22_001331"
     CDS             complement(10318..11004)
                     /locus_tag="NBK22_001331"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCD2414522.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(11331..12197)
                     /locus_tag="NBK22_001332"
     CDS             complement(11331..12197)
                     /locus_tag="NBK22_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD2414523.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVEAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(12735..12854)
                     /locus_tag="NBK22_001333"
     CDS             complement(12735..12854)
                     /locus_tag="NBK22_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414524.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQYFANK"
     gene            12949..14259
                     /locus_tag="NBK22_001334"
     CDS             12949..14259
                     /locus_tag="NBK22_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B domain-containing protein"
                     /protein_id="HCD2414525.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDKSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     TKLQKNDSIENRRLAQREVNKAPMDVKEHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYTDKYFKYKGTIDKTVLSVFGNGYKSYIQPLKVEDQKNYVSKSYAQVRNYVT
                     ETLNTGKVLYAFYQNPKVVKAAITTAETASSIKNTLSNLLSFWK"
     gene            14795..15724
                     /gene="hlgA"
                     /locus_tag="NBK22_001335"
     CDS             14795..15724
                     /gene="hlgA"
                     /locus_tag="NBK22_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCD2414526.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHERGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
BASE COUNT         5428 a         2868 c         2166 g         5387 t
ORIGIN      
        1 atttgaaaag taatgtaaac catatgcaaa tataccgtag cctacgactg tacgcaaaat
       61 aaatctaacc actttatttg gtacacattt cgaagtaaca gtaattgaag ataaaagcgt
      121 agtaaaaata tataatatta atactttaat gtaacgttgt gccatcgaat catccttcat
      181 tcaattaata atattacgtt attattataa actgaatggc atataaaatt ttttacatat
      241 tttcaacaac tttatttcaa taaagcatca agttgatcta aagtttgatt cataccttgt
      301 tcaacaccca tgtttatgac ttgttgagcg gcttcttttg ttggaaatac cgatgttgat
      361 gtcactgtcg ttttcgttgt attgctcttg gaaaatgaca aagtaatttt catgcttggc
      421 atttttgtat cttttgcacc ttgaggtgtc gcaaatgagt caatatattc aattaaatat
      481 ggacgcttaa ccgttttata ttctgctaat gtatagctgg tcattgtagg tgttttaatc
      541 gcataaaatg catcaccgcc tgaaacagca ttaaagcgaa acactttggt actagcgtct
      601 tttggatgaa accatttttc aaataatgct tttgtcgtat aggcatcaaa caccttttca
      661 atcggtgctt ctatcgttct tgaaaaaata attttattat cttcaacttt aatcgtcatc
      721 ttcccactcc ctatctttta tatttactca tcttaacatg cattgagtaa catatgttac
      781 caaatcattt gttataaact atagtaatat taatcattcc ctctgttgaa attgataact
      841 acgaaataaa aaacacactc tattcagcta ttaacagttg agtagagtgt gcaaacctgt
      901 tgaattaatg cgctaacatt tcttctttga tttggccttt atttaatttt gaaggataat
      961 atgttggcca gttatccatt tcttttaata acacttccat atcattaccc ataaagatat
     1021 ggaagtgttc tgccttttta ggtgcaatgt tgtgatcgct aaattggatg aatttcggta
     1081 agtctttatc attaccatcg accaatttaa acataaatct cacgccacgg ttacctttag
     1141 gatatttcaa tgttttctta ccattgtatt catatttacc agtgtgtttc ttaccatctt
     1201 tagtaaatgt aatttcattt ccttttatat caatgttagt aatatcagtt ttatatcctt
     1261 tgtcataata agcttttaaa tctttagcag atttcttcgg atcattttca gctttatgtt
     1321 ccatcacttc atcaagcgta ccgtctttta agtaagggta aacagattgc cattcaccag
     1381 cataatcgct taattcacgg tctttaactt gctcatcttt aaaataccca tcagaaattg
     1441 ctttgtcatg tttactttca gctttgtcgt ctttcacttt aacaccactg tctaaagctt
     1501 taccgatatt ttcaatatta gatttcatta atgattgata cgtaatatta tcttttttct
     1561 gttgttcttt atttaaagac tccatgttgt agaattttaa aggcttcgca tctgtttctt
     1621 tacgaattgt ttcagtcact ttattcgcaa cattatcttc gtaaagaata tatttagcat
     1681 tgctatctct aatttcttta acaattttag ttaattcttt ttgtgatgga tcttcagcat
     1741 tcatgttttg aatacctttt tgaacaaaac cataacgatc agctaagtat ccaattgatt
     1801 catgtgaaat gaatactgca ttaccttgct tatcttttgt aacttgcttc atatcgttat
     1861 caattttctt aagatcgtcg tttaattttt tgtagttttt ctcatagtca tctttatgtt
     1921 ttggatcttt tttcactaat tcatctttaa tttctttagc gaaagtttgg ttaattttag
     1981 gatctaacca tacgtgtgga tcatatccac catgatgatg atgttcttct ttctcatgat
     2041 catgtccctc atgttcatgc tcttcaccat gctcgtgttg atcagttaaa agctttgctt
     2101 tatctaattt atcctctaaa gacagttttt tatctttatc tttgatagta gatgcaactt
     2161 tcttagcaac cggatctaaa ttatcccctg tatacataaa caagtctgac ttgcttgcac
     2221 ttaatatatc tttttgtgtt ggttcatagc tatgtaaatc tgtccctgct ggatagattg
     2281 atgatacctt cacgtgtttt ccaccaattt gctctgcaaa tgattgcaat ggataaactg
     2341 tcgttttaat tgttaccttg ccatctttat cttttccatc atcattccca catgcggcta
     2401 atgataaagt tacggctggt acaagaagta acatacctaa tttctttttc atagtcaccc
     2461 tcctaaatag taatctttac gttttaaaat tatatactca tgctacttaa aatacaagca
     2521 ctgtttttat aaataatata aagtcgaatc atactttagg tatttaccac ttttcgtttt
     2581 atctaataca tgactattgt acaaatatga tacagaaaac tttatagtat ttacgaacgt
     2641 cataatataa gaaaaaaaga caatctctat catattgcta atagaaattg ccttttcata
     2701 ttgtcctaaa ataacttacc ctatttttat agtgtgactt ttagatttaa taatggtatt
     2761 tacatgatgc tatttttata tgattgtcat ctactgtata aaccaatcta tgttcgtgat
     2821 tgatacgtct actataatac ccagtcagat tcgattttaa cttttcaggc ttacctatac
     2881 cttccaatgc accatttctg tcaatacttt caagtaactc attaatcttc ttcaccattt
     2941 ttttatcgtt ctgctgaaaa tacttataat cttcaaaggc ttgaggcgaa aacgtaatat
     3001 ttaacctagc cattatacat ctatatcttt cgttatagtt ttcccacgtt ctagatctgc
     3061 aattgattga gctaagtgtt cagcattatt tgggttctgt tggaggtaaa gtgtttccat
     3121 catggagtta taatctgatt ctgacatgat tactacgttt ttatcatcag tagatgttac
     3181 agttaccata tcactatcat catttacttt tgtcataagt gcctttaaat tctgtcgagc
     3241 gtatgaataa tttttaataa tcatatttat cgcctccttt taacacacct caattgtaca
     3301 aatatctgta caataaaaca agcgacacag aaaaaaacta ctaatcaaga tttcgcacgg
     3361 ggaagaatca atacctatcc tgtactgatt ccctactaaa caatgtttac atgataattt
     3421 ctacctattt agacttcata cattaaaaag acaatctcta tcatatcgct aatagaaatt
     3481 gccttttcat attgtcctaa taaattgata tcacttactt atctcaacaa ttaagactat
     3541 tttattttat cttttaataa cttctcataa ctttcataat catatggatc ttcaactttc
     3601 tcgccattaa taaatgcagt tggtgtcgtt tttatatgat tatctttcgc tattttttta
     3661 tctttctctg cagctttcca agacttgcta tcttttgtct tgtaatcttt tataatttta
     3721 ttttccgtct ctttatctaa atgcagttgt ttaatatgtt tatctaatag ttcttttgtt
     3781 aaccattctt tattttcatc ttgctgggca gcaaataatt gcttttgaaa atctaaaaat
     3841 gatttaggtg catacatcaa tactgcatga ctcgcacgcg aaccaacaat tgagtcttta
     3901 cctaagaaag ctaaattgac aaattggtat tccactttgt gattatctat ataattttta
     3961 cgcaactttg gcatgacttt ttcatctaat tctttacaat aaggacattt atagtcgcca
     4021 tatatgacaa ctaatggttt gccgtttttc gaggatgtcg ttgctgattc ttttttaccg
     4081 caagctgtta aaattaacat gctcactata aataatgtta gtaatttttt agtcataata
     4141 actcctgttc atatctagtt cgtcatattt tcttcatgat aatccgcatc atgttgttga
     4201 taatatcgtc tcgaatcaaa atcttgctta tatgatacac gtttatcact aaaatcatat
     4261 gcataataat gcacttcatc atcattactc aaaggtttat aagctaaaat gataatttta
     4321 tccttatcat agacataaat attagcatct ttgcgttcaa aatgatcaac acgatcaccg
     4381 ttgccttttt tggcaagttg ttcttgtttt tgattttgaa ttttaactgc ttcatctatt
     4441 tctttcacat atttatcatt accacatcct gatataaaga ctagtaataa taataccgtc
     4501 gccacaaatc gcttcaatgc tcccactcct actatcaact tccaactaac tttcaatata
     4561 attaatacta tacaccgtca aacattaatg cgctacaact actaccaatg catttaatat
     4621 aaatatccaa agaaataaca gttatgtata gtcaaagaaa aatatatttc attttctatg
     4681 tgtatttgta atcagttttt gataaaataa aaaaatagca ttattaataa attcaaaaat
     4741 tacagttcaa ttcagagagg gatgggataa aatgaacttt gtaacgttga cttcggatga
     4801 gttcaatgcg tttacaacaa agcatttttc acattacaca caatcagcta ttcattacaa
     4861 tcatagagtt gatttaaaag gcgatgtgca tcttgtaggg gttaaagatg acaacgaaga
     4921 agtagtagca gcctgcttac taacagaggc acgtacactt aagtttttta aatatttcta
     4981 tacacatcgt ggaccagtga tggattatac aaatcaatca ttagtgtcat ttttctttaa
     5041 atcactaact tcatatttga agaaacaaaa ttgtttatat gtacttgtag acccatattt
     5101 aatcgaaaac ttacgtaatg cagatggtga aattgttaag tcttatgaca accgagcatt
     5161 tgttagaaca atggacacat taggctataa gcatcaaggt ttttcagtgg ggtatgattc
     5221 gatgagtcaa atccgttggt tatctgtatt agatttaaag gataaaacag aagaccagtt
     5281 attaaaagaa atggattatc aaacgagacg taatattaaa aaaacatatg atattggtgt
     5341 caaaactaaa acgttaacga ttgatgaaac gcaaactttt ttcgacttat tccatatggc
     5401 cgaagaaaag catggtttca aattccgtga gttaccatac tttgaagaaa tgcaaaagtt
     5461 atacgatgac cacgccatgt taaagttggc gtatattgat ttaaacgagt atttaaaaac
     5521 gttacaatta aagcaacagc aattaacggc tgaactctca ggtgttgaag aagcattgga
     5581 agaaagtcct aattcaaaga aaaataaaac gaaacgcaca cagctagaac aacagctaaa
     5641 tagcaataag cgtaaaatcg acaatacaat agaacaaatt gaacaagatg gtgcagtctt
     5701 aaacttagct tctgctttat ttatctacaa tgagcatgaa gtttattact tatctagtgg
     5761 ttcaaaccct aaatataatg cttatatggg tgcctaccat ttgcagtggg aaatgattaa
     5821 gtttgctaaa gcgcatcata ttgatcgcta taatttttat ggtattaccg gtgacttttc
     5881 agagtcatca gaagactatg gcgtccaaca attcaaaaaa ggttttaacg cacatgtaga
     5941 agagtatatc ggtgacttta ttaagcctat caaaccatta ctatataagg tacaaacgta
     6001 cttaaatcat aaacgtcgtt aaaatatcgc tccaagattt tggcaacata acaaagaacc
     6061 acgcatcatg tatatggcat catcaccata ctaaaacatg atacgtggtt ttatttgatt
     6121 attcttcatt tataacattt aagaaacgtc ttaattcttc tgttttcgga cgattgaaca
     6181 tctcttctgg agccccttgt tctccgatta tgccttcatg aataaataca atgttattag
     6241 atacttcttt agcaaaacgc atttcatgtg tcacaatgac cattgtcatg ccttcattag
     6301 ccaaatcttt aataaccttt aaaacatcat tcacaagttc aggatcaagt gcagatgttg
     6361 gttcatcaaa caacatcact ttagggttca ttgctagtgc tcttgcaata gcaacacgtt
     6421 gttgttgacc acctgataat gcatgtggac gttgatcttt gacatgtgtt aaaccaactt
     6481 tctcaagtaa ctcaagtgat ttcccacgtg cctcatcctt tttcaacttt ttaactgtga
     6541 taagaccttc cattacattt tctaatgccg tcttatgcgg aaaaagatta taactttgaa
     6601 ataccatacc agactgttta cgaacttcta tttgtgattt tttatcttta gatgtatatg
     6661 ttttgccgtt aacataaact gtaccttcag ttggaatttc taatgcatta atcatacgta
     6721 acaatgttgt tttgcctgaa ccagatcgac cgattaaggt tacaacctca ccttgttcaa
     6781 cagataaatc aatacctttg atgacttcaa catcattaaa tgatttatgg atattgttca
     6841 attgaatcat gagcgatacc ctctttcaat ataagattca tagatacctt ggataattga
     6901 aataataaag cataccaccc agtacattag cgcaactaac acataaattg ttaaatattc
     6961 atatgttgta gacgcaactt cttgagcttt tctaaacatt tcagccacta aaataaatcc
     7021 taataatgat gtatctttga ttaaacttaa aaatgtatta cctagtgcag gaatcgacac
     7081 tcgaattgcc tgcggtaaaa tgatacgttg tatcgtttga ccatacgtca taccgatgga
     7141 atatgcagct tctgtttgtc ctttcggtat agaaataata ccgccacgaa taatttccga
     7201 agcatacgca cctacattta atgataaacc aataatagca gccactacag gtgccaatgt
     7261 ccattggttg tcagcgtcat ttgttaataa tctacctaat tctggaatac cataaaatat
     7321 gataaatagt tgtactatca ttggtgttcc tcgaataata gatacataga cacgtgaaat
     7381 acttcttaaa attttactcg ttgaaattcg cattaatgct gtaaataatg caataatcaa
     7441 tcctaaaaca aatgtaacta atgtaatagg aattgaatat ttgaccaaac cttctagcat
     7501 aggtccgaaa gcttgttttg cagcatctag cgcatgttgt tgttcgctat ttagatttag
     7561 aaacatcttg accaaaccat ttcttaccta ttttagctaa ttcaccgttt tcctcgattt
     7621 ttttcaagcc atcattgaat ttttgaactg tttcatcatc tgctttttta gaaaatgcaa
     7681 atgcagatct actttgttca gcattacctt tgattgcttt gatcttagca ttaggttttt
     7741 gttttttata atccaagtat gacagactat cattaaatgt accatcaaca cgcttagaca
     7801 ataataaatc cattgattgg ttaaagccat caacttttgt aatatcagca cctttatcct
     7861 tagctaattt accataatta gatgtgaatg tttgtgctaa cttcttacct ttaacatcat
     7921 caaaatcttt aatatctttt tcattttcac gaataactaa aacagcactt gagaatgtgt
     7981 aaggcttaga aaatttatat ttcttttctc tatcaggatt aatacctact tggttcgcga
     8041 ttacatcaaa acgccctgcg tctaaacctg caaacataga atcccaagaa gtttcattaa
     8101 atttaagttt taaaccttct tctttagcca ctgctttaat aacatcaata tcgtaaccag
     8161 ttaatttacc atctttatcg tggaaactaa atggtgcata agtcccctca gtcccaacat
     8221 taattgtctt gctatcttta ctagcttcct tgtctttcga cgaattgttt ccgcatgcag
     8281 ccaatatgaa aacgaaagct atcatcacaa ataaaagtct tttcatagaa tatatctcct
     8341 tattcttatt attctaatcg gaataaaaat attttaatct gaaatgccgg ataagtcaat
     8401 atgttgaact tttgctaaaa agctttatat agctagcaac ttatatttat tgacgagaat
     8461 caacttcttt tttcttacct tgtaacataa atgaacaaaa gaaactaaca actgtaatca
     8521 ataatgcaaa taaaaatcca gcgtgataac catgcaacat cgtatcaact ttaatttgat
     8581 tcatcatatc aattttgttc atgccattat agtctgacat agttggagaa aagtttttgc
     8641 ttgcatggga taaaatagta attaatgcag ctgttccgat agaaccagca atttgttgta
     8701 ctgtgtttgt catagatgaa ccatgtgcat tcatttctgg ctttaattga ttcatcgtat
     8761 gggtcattac tggaattaat cctaacgcga taccaaccat tcgaatcgca taaactgttg
     8821 ccaacatgat tgttgatgtt tgttcatcca ttacaacaaa ataagccgta gtaataacaa
     8881 caatcgccat accgactaaa gccaattttc ttggaccaaa cttttcatat aaagcacccg
     8941 aagtcatcga cataaatgcc ataacaattg ctcctggtaa taaagttaac ccagaatcta
     9001 aagctgaacg atgcaataaa ttttgcacaa agataggtaa aatcgtttcc gaaccaatca
     9061 tcgataccat tgtgacaccc ataataatga taccaactgt aaattgttta tttttaaata
     9121 cagcaaaatt taataatggt gttgatagcc gatattgacg taatacaaat aatgcgacta
     9181 aaacaacgcc tccaatcata gatactaata cgataggatt atcaaaacct ttttctgaaa
     9241 ttgaactcgt tccatacaat aacccgccga aacctagtac agaaaaaatg acagaaataa
     9301 aatcaatagg tactttaatt gtagttccaa cattttttat cgttttaaat ccaattaaaa
     9361 atgtcacagc tgcaattgga gcgacaacat ggaaaagcga tctccagtta aaatattcaa
     9421 caaggtaacc tgaaagagta ggtccaatag ctggtgctaa accaattacc aaaccaaata
     9481 tccccatata tttaccgcgt tcatgcggtt ggaaaacatc caaaatcgtt gtcatcatca
     9541 atggcatcat aatacctgcg cctaacgcct gaataattct agcaactaat aatgttgcaa
     9601 agtgcggact caagccgccg agtaatgtac ctaagaaaaa gatagaaata cccactaaaa
     9661 acacttgtct tgttgtataa cgttgtataa caagcgcgga caaaggaata acaatcccat
     9721 tagtcaataa aaaagctgtt gtcagccatt gaacttgtgt atatgaaatt tcaaaatctt
     9781 tcataatact tggtaaagcc gtcactaata gtgtttcatt taataatcca aaaaatccac
     9841 ccatcagcat agtaataatc atcgttaatt tttgtttctt cgacatttaa agcttccccc
     9901 tcaccatcta aaaaacgaca cacattttta gatatccggt aattataccc attttccaaa
     9961 aaattagcac gactttatta caatttcttt aaaacgtaat cattactatt tacatatgag
    10021 tagttatcgg tcataatata tttacaagga gggatagaga tgaaattaga tttacagact
    10081 gctcgtcgaa atttaaatag ccctaacatt aaaacaagaa agcgtgcttt aaagattatt
    10141 aagcaacaca aaagagcgaa ataattgttt taatttgact atattgtatg tctaactata
    10201 ttttcgagta tccctagctc gttaatataa actctatact taactatttc cttacattac
    10261 ttcacatatt taagagctgc atatacactc actccttgtg tacatgcagc tcttttttta
    10321 taagtagtat ttatctataa cttctaaatc atccgttaat tcataaacaa gcggtgcacc
    10381 tgttttaatt tcataattaa tgatgtcttc atctgacaca tcttcaagat atttaattaa
    10441 tgcgcgaatt gaatttccgt gtgcagaaac taataccgtt tgaccatcta gcaaatattg
    10501 tgaaatatga tctgtccaaa atggtatcac tcgaactaaa gtatctttca gactttcaga
    10561 ataaggcatc atacgtttat ctaaatgatt atatcgacga tcagctaagt aagcttcacg
    10621 ttgttcttcg gtttcagcag gcggtttcac atcataagaa cgacgccaaa tatgtacttg
    10681 ttcttctcca aattcttttc tagcatcatc tttatttaag ccttgcaatc caccatagtg
    10741 gcgttcattt aaacgccagc ttttatatac aggaatccat tgttgtttag attcagttaa
    10801 aatgtaatgc gttgtatcta aagcacgtgt taataacgat gtaaaagcta catcgatggc
    10861 gatgttattt tctcttactt tttcacctgc tctagtcgct tcattaatac cttgttcaga
    10921 taaattaaca tcttcccatc cagtaaataa gtttttagca ttccactcgc tttgtccatg
    10981 acgacataaa attaattttg gcataactta taacctcttt cctttaatct ttaggacgtt
    11041 atttcaggga tattttaaga tgatttaatc ttagtaattt tcttttcaat atattttaaa
    11101 attcaatgat taaatcaatt atcactagac attaaataca taaatcctat tccccatttt
    11161 cattttttaa ttcataaatg aatcaataac cacctaataa caaatcatat tatacacctt
    11221 tatccgctat ttttctaagt tttaaaaaat atttttaggt gcacctaaaa ataaatgtaa
    11281 taaaaacgcc tcctcagata tttatatatc tatgaagacg tttaaataca ttatagatgg
    11341 tctggttctg ggtgaacgta tactgaggaa ataccttttt tgtgcaaatg atgttcgaca
    11401 ttgtcacaaa tttgatgcgc ttctactaag gaaaggttag catctacaac aattgtgaca
    11461 tcaataaaca cactacttcc atggtaacgc cctttaatac ttttaacttc ttgtacttca
    11521 tcaacttcta aaatatcatt gcgatacgct tctaattcag tttcattgaa accatcactc
    11581 aacataaaaa ttgcttcttt aaaaatacca aaaccagtat aaacgattag taagcctagt
    11641 aatgttgcta aaataatatc gacaattggg aaaccgattt gcgtaaaaat taatcctatc
    11701 gctgttccaa tgctgactaa actatctgat aaattatctt tggcagccga atttaaagaa
    11761 ctacttttcg ttcttttcgc tagtctttga ttgactgcaa atacaatcaa cattacaaga
    11821 ccactgatta agctgacgat aattgttatt gcgttaggta caacgtcatc ttctttgaac
    11881 aaacgaggtg cattttgaat aactacttgg atacctacaa acataatgac aaatgacacc
    11941 aataatgaag aaatattttc agacttcaaa tggccataag gatgatttcg atctgcaggt
    12001 ttaattgaaa ttttcaatcc aataataaca gctaaagaaa cgataatatc tgtcatattg
    12061 tttaatgcgt cggctcttac agctgcagag ttaaagacaa aacccgtgac atacttaaca
    12121 atagataaga ttatatatac aattaaactc aaataagcac cgcgttgcgc caatttaaga
    12181 ttttcattat gagacatgcg ttgaaccacc ttgaattagt atagtaacaa tattatgaat
    12241 gatttatttt aattttacaa cgtttttaat ttttataaat ttttataaaa ttaaactaat
    12301 ttattcattc gcaaccccta aaaataattt ttagcctttc tgcgaatttt atgagctaga
    12361 aaggcttatg cagttgacgt tttacgtcca actgcgttcc ttcgtcttct tcaaatttat
    12421 ttgttagaaa ggcacccaac ccttcctgct tgttaacttt cgtctcgaaa gtttctatgt
    12481 tgggacccaa tgcattagta gcgaagctat ctaatgcgtt cctttatttt ctgcgaattt
    12541 tatgagctag aaagacgccc aactctccct gtttgtgaac tttcgcctcg aaagtttcta
    12601 tgttagaacc ctatgcatga gttgcgaagc tatctaatgt cgtgacctaa ttatatagaa
    12661 gaaaaagtgc atcagtgaca aattaaatga gatttctact ctaccaaact ctcttcgaaa
    12721 gacaattttc tcctctattt attagcaaaa tattgcattt ctccatatag tacttcctta
    12781 cttaaaatac gctgaatgtt ctgaattaaa cgctttttta catagttaac actagttaat
    12841 ctattagtta acattagtta ataattagtt aatttccatt tgtattctca tgtgataaat
    12901 tctaaaagca tacaataaat ttaatatgta aaaagaaagg gaatacacat gaaaaataaa
    12961 tatatctcga agttgctagt tggggcagca acaattactt tagctacaat gatttcaaat
    13021 ggggaagcaa aagcgagtga aaacacgcaa caaacttcaa ctaagcacca aacaactcaa
    13081 aacaactacg taacagatca acaaaaagct ttttatcaag tattacatct aaaaggtatc
    13141 acagaagaac aacgtaacca atacatcaaa acattacgcg aacacccaga acgtgcacaa
    13201 gaagtattct ctgaatcact taaagacagc aagaacccag accgacgtgt tgcacaacaa
    13261 aacgcttttt acaatgttct taaaaatgat aacttaactg aacaagaaaa aaataattac
    13321 attgcacaaa ttaaagaaaa ccctgataaa agccaacaag tttgggtaga atcagtacaa
    13381 tcttctaaag ctaaagaacg tcaaaatatt gaaaatgcgg ataaagcaat taaagatttc
    13441 caagataaca aagcaccaca cgataaatca gcagcatatg aagctaactc aaaattacct
    13501 aaagatttac gcgataaaaa taaccgcttt gtagaaaaag tttcaattga aaaagcaatc
    13561 gttcgtcatg atgagcgtgt gaaatcagca aacgatgcca ttactaaatt acaaaaaaat
    13621 gattcaattg aaaacagacg tttagcacaa cgtgaagtta ataaagcacc tatggatgta
    13681 aaagagcatt tacagaaaca attagacgca ttagtagctc aaaaagatgc tgaaaagaaa
    13741 gtggcgccaa aagttgaggc tcctcaaatt caatcaccac aaattgaaaa acctaaagca
    13801 gaatcaccaa aagttgaagt ccctcaaatc caatcaccaa aagttgaggt tcctcaatct
    13861 aaattattag gttactacca atcattaaaa gattcattta actatggtta caagtattta
    13921 acagatactt ataaaagcta taaagaaaaa tatgatacag caaagtacta cactgataaa
    13981 tattttaaat ataaaggtac aatagataaa accgtactat cagtttttgg taatggatac
    14041 aaatcataca tccaaccatt aaaagttgaa gatcaaaaaa attatgtatc taaatcatat
    14101 gcacaagtaa gaaactatgt aactgaaact ttaaacacag gtaaagtatt atatgccttt
    14161 tatcaaaatc caaaagtagt taaagctgct ataactacag ctgaaactgc atcatcaatc
    14221 aaaaatacat taagtaattt attatcattc tggaaataat caatcaaaag tatcttctct
    14281 agttttacat cattttttaa ataattttcg taataaaccg tgattaaaaa gaaccgtaga
    14341 ttctcaatcg aatctacggt tcttttttca ttatccatca attaaatgtt tcttcgctat
    14401 ttgtcatccc acttttttat ctgcgacttg ttaaataatc cttacatcgt taacgaatag
    14461 ttcatcattt agttgaatca gctcaacttt attaacttca aattttcaca aactgttgcg
    14521 caatccattc ctcttccact acaagcacca taattaaaca acaatccaat agaataagac
    14581 ttgcaaaaca tagttatgta gctatataaa cgcctgcgac caataaatct tttaaacata
    14641 acataatgca aaaacatcat ttaacaatga taaaaatgtc tcttcaatac atgttgatag
    14701 taattaactt ttaacgaaca gttaattcga aaacgcttac aaatagatta ttatatatat
    14761 gaacttaaaa ttaaatagaa agaaagtgat ttctatgatt aaaaataaaa tattaacagc
    14821 aactttagca gttggtttaa tagccccttt agccaatcca tttatagaaa tttctaaagc
    14881 agaaaataag atagaagata tcggccaagg tgcagaaatc atcaaaagaa cacaagacat
    14941 tactagcaaa cgattagcta taactcaaaa cattcaattt gattttgtaa aagataaaaa
    15001 atataacaaa gatgccctag ttgttaagat gcaaggcttc atcagctcta gaacaacata
    15061 ttcagactta aaaaaatatc catatattaa aagaatgata tggccatttc aatataatat
    15121 cagtttgaaa acgaaagact ctaatgttga tttaatcaat tatcttccta aaaataaaat
    15181 tgattcagca gatgttagtc agaaattagg ctataatatc ggcggaaact tccaatcagc
    15241 gccatcaatc ggaggcagtg gctcattcaa ctactctaaa acaattagtt ataatcaaaa
    15301 aaactatgtt actgaagtag aaagtcagaa ctctaaaggt gttaaatggg gagtgaaagc
    15361 aaattcattc gttacaccga atggtcaagt atctgcatat gatcaatact tatttgcaca
    15421 agacccaact ggtccagcag caagagacta tttcgtccca gataatcaat tacctccttt
    15481 aattcaaagt ggctttaatc catcatttat tacaacattg tcacacgaaa gaggtaaagg
    15541 tgataaaagc gagtttgaaa tcacttacgg cagaaacatg gatgctacat atgcttacgt
    15601 gacaagacat cgtttagccg ttgatagaaa acatgatgct tttaaaaacc gaaacgttac
    15661 agttaaatat gaagtgaact ggaaaacaca tgaagtaaaa attaaaagca tcacacctaa
    15721 gtaaacagtt caatcattta aaaaaatcct gggacacttc atacttgtct caggattttt
    15781 caacatattg aatcagcctc ataacattaa attaatttat cgtacattaa atttaataat
    15841 aacaactga
//
LOCUS       DAHHYJ010000057        15178 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000057 DAHHYJ010000000
VERSION     DAHHYJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15178
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.057"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            123..1148
                     /gene="gap"
                     /locus_tag="NBK22_001336"
     CDS             123..1148
                     /gene="gap"
                     /locus_tag="NBK22_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD2414527.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            1359..1829
                     /gene="nrdR"
                     /locus_tag="NBK22_001337"
     CDS             1359..1829
                     /gene="nrdR"
                     /locus_tag="NBK22_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCD2414528.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            1830..3230
                     /locus_tag="NBK22_001338"
     CDS             1830..3230
                     /locus_tag="NBK22_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCD2414529.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPATSSSTGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYFGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            3230..4150
                     /gene="dnaI"
                     /locus_tag="NBK22_001339"
     CDS             3230..4150
                     /gene="dnaI"
                     /locus_tag="NBK22_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCD2414530.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNQRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            4243..4365
                     /locus_tag="NBK22_001340"
     CDS             4243..4365
                     /locus_tag="NBK22_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414531.1"
                     /translation="MFDENMVNKLDDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            4562..6499
                     /gene="thrS"
                     /locus_tag="NBK22_001341"
     CDS             4562..6499
                     /gene="thrS"
                     /locus_tag="NBK22_001341"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCD2414532.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            6925..8418
                     /locus_tag="NBK22_001342"
     CDS             6925..8418
                     /locus_tag="NBK22_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2414533.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     misc_feature    8493..8618
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8647..9174
                     /gene="infC"
                     /locus_tag="NBK22_001343"
     CDS             8647..9174
                     /gene="infC"
                     /locus_tag="NBK22_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCD2414534.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     gene            9203..9403
                     /gene="rpmI"
                     /locus_tag="NBK22_001344"
     CDS             9203..9403
                     /gene="rpmI"
                     /locus_tag="NBK22_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCD2414535.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            9450..9806
                     /gene="rplT"
                     /locus_tag="NBK22_001345"
     CDS             9450..9806
                     /gene="rplT"
                     /locus_tag="NBK22_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCD2414536.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            9948..10556
                     /locus_tag="NBK22_001346"
     CDS             9948..10556
                     /locus_tag="NBK22_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD2414537.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            10575..11504
                     /locus_tag="NBK22_001347"
     CDS             10575..11504
                     /locus_tag="NBK22_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414538.1"
                     /translation="MSDIIQFPNSSKKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DNGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLN
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            11509..11619
                     /locus_tag="NBK22_001348"
     CDS             11509..11619
                     /locus_tag="NBK22_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414539.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            11667..12968
                     /locus_tag="NBK22_001349"
     CDS             11667..12968
                     /locus_tag="NBK22_001349"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCD2414540.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            13119..14381
                     /gene="clpX"
                     /locus_tag="NBK22_001350"
     CDS             13119..14381
                     /gene="clpX"
                     /locus_tag="NBK22_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCD2414541.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            14535..15125
                     /locus_tag="NBK22_001351"
     CDS             14535..15125
                     /locus_tag="NBK22_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCD2414542.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS"
BASE COUNT         5632 a         2068 c         2923 g         4555 t
ORIGIN      
        1 taagtaatga ctatttttta taaaaaagat aaataaatca acggaaaacg ctttcaaatt
       61 tcatataata tgctatacta attccataaa gtataacaca taaagatcaa ggggtgcttt
      121 taatgtcaac gaatattgca attaatggta tgggtagaat tggaagaatg gtattacgta
      181 ttgcattaca aaataaaaat ttaaatgtag tagcgataaa tgctagttat ccacccgaaa
      241 caattgcaca tttaatcaat tacgatacga cacatggaaa atataatcta aaagttgaac
      301 cgattgaaaa tggattgcaa gttggagatc ataaaattaa attggttgct gatcgcaatc
      361 ctgaaaactt gccatggaaa gaattagata tcgatattgc tatagatgca actggtaaat
      421 ttaatcatgg tgataaagcc atcgcacata ttaaagcagg tgccaaaaaa gttttgttaa
      481 ctggtccttc aaaaggtgga catgttcaaa tggtagttaa aggcgtaaat gataaccaat
      541 tagatataga agcatttgac atttttagta atgcttcatg tactactaat tgcattggtc
      601 cagttgcaaa agttttaaat aatcagtttg gaatagttaa tggtttaatg actactgttc
      661 acgctattac aaatgaccaa aaaaatattg ataatccaca taaagattta agacgtgcac
      721 gttcatgtaa tgaaagcatt attcctactt ctactggtgc ggcgaaagct ttaaaagaag
      781 tattaccaga attagaaggt aaattacacg gcatggcatt acgtgtacca acaaagaatg
      841 tatcgctcgt tgatttagtt gttgatttag aaaaagaagt aactgcagaa gaagtaaacc
      901 aagcttttga aaatgcaggt ttagaaggta tcatagaagt cgaacatcaa ccactagtgt
      961 ctgttgattt taatactaat ccaaattcag ctattattga tgcaaaatca acaatggtca
     1021 tgtcagggaa taaagtaaaa gttattgctt ggtatgataa tgaatggggt tattcaaata
     1081 gagttgtaga tgttgctgag caaattggag cacttttgac atcaaaagaa actgtaagtg
     1141 caagttaata caattaattt atagcctaac tctgacttcg gagttaggtt ttttattttt
     1201 taatacaata atattgtcat gcaaattact taataagtaa cgatataatg ttttttattg
     1261 tttcattatt tggttaaatg aattaaattg agggattctt ttttcatctc tagatttata
     1321 gtataattag gcataatcaa aataaagagg gtgatgtaat gaaatgcccg aaatgtaatt
     1381 ctacacaatc taaagttgta gattcaaggc atgccgatga attaaatgcc attcgaagac
     1441 gaagagaatg tgaaaattgt ggaacacgtt tcactacatt tgaacatatc gaagttagtc
     1501 agcttatagt tgtgaaaaaa gatggcacaa gagagcagtt ttcaagagaa aagatactta
     1561 atggacttgt gcgttcttgt gagaaacgac cagttagata tcaacaactt gaagacataa
     1621 caaacaaggt tgaatggcaa ttacgagatg aaggtcatac ggaagtgtct tcacgagata
     1681 taggtgaaca cgttatgaac ttgttaatgc atgttgatca agtttcatat gttagatttg
     1741 catcagtcta taaagaattt aaagatgttg atcaattgtt agcatcgatg caagggattt
     1801 taagcgaaaa caaacggagt gatgcttaaa tgggacgaca agccttcgaa ttcggcttaa
     1861 gaccaaagga tcaattcaaa gttatgcaac actttgattt aaatacgaat catttagagg
     1921 tattgaatcg gctgtataca ccgctaattg gaacacaagc agtgggttta taccatttta
     1981 tgactcaatt cgttaaagaa agtcataatg aaacattgat tctttctcat tatattttta
     2041 tgaatgaact taaaattaat ttactagagt ttagacaaca aatggactta cttgaagcga
     2101 ttggattgtt aaaggcgttt gtcaaacatg atgaacaaga aacacaattt gtttaccaat
     2161 taattcaacc gccttcagca catttatttt ttaatgatcc aatgttgtca atctttttat
     2221 atagtgaagt cgagcatcgt cgatttcatg agttgaagaa atactttgag tatcaacaaa
     2281 ttgatttgag tgaatttaaa caggtaacaa gacaatttac tgatgttttt aaagtgccgt
     2341 ctacaaaaat agatatcgat acaagtgata ttccgattaa tgaaccgtac caaggtatcg
     2401 atttatcaaa tgaatctttt gactttgaaa tgttgagaca aatgttaggt aaacatttta
     2461 ttagtcaaga cattgttacc aaagatgcaa agcgtttaat aacacaactc gcgactttat
     2521 atggtttaac agcagatggc atgaagcatg ttattttaaa ttcaattaca agtggacaac
     2581 agttatcctt tgaagaaatg cgtaaacaag cacgctcgta ttacttaatg gaacacgaaa
     2641 atcaaatgcc taaattacag gtgaaatctc ctgctacatc atcttcgacg ggcaaatctt
     2701 cagaagtgaa tccaaagcca caaagtgatg aatggtttga gttacttgaa caaacgagtc
     2761 caattgatat gttagcttct tggtctgaat cagaaccaac tatatcgcaa aagacaatgg
     2821 ttgaagaatt aattgaacgt gaaaaaatgt cttttggcgt cattaatatt ttgttgcaat
     2881 ttgtcatgtt aaaagaagat atgaaacttc ctaaagcgta tattttagaa atagcatcaa
     2941 attggaagaa aaaaggtatt aagacagcta aagaagcata taattacgct aaaaaagtta
     3001 atcaacctaa aaatgagggt tctagcggaa attaccaaaa gcgtggttca tactttgggc
     3061 agcgtaatcg tatttcaaaa gaaaaaacac ctaaatggtt agaaaataga gataaaccta
     3121 gtgaagaaga ttcggctaaa gataatagcg tagatgatca acaattagag caagatcgac
     3181 aagcatttct agataaatta tctaaaaaat gggaggagga cagtcaataa tgaagcaatt
     3241 taaaagtata attaacacgt cgcaggactt tgaaaaaaga atagaaaaga taaaaaaaga
     3301 agtaatcaat gacccagatg ttaagcaatt tttggaagcg catcgagctg aattaacgaa
     3361 tgctatgatt gatgaagact taaatgtgtt acaagagtat aaagatcaac aaaaacatta
     3421 tgacggtcat aaatttgctg attgtccaaa tttcgtaaag gggcatgtgc ctgagttata
     3481 tgttgataat aaccgaatta aaatacgcta tttacaatgc ccatgtaaaa tcaagtacga
     3541 cgaagaacgc tttgaagctg agctaattac atctcatcat atgcaacgag atactttaaa
     3601 tgccaaattg aaagatattt atatgaatca acgagaccgt cttgatgtag ctatggcagc
     3661 agatgatatt tgtacagcaa taactaatgg ggaacaagtg aaaggccttt acctttatgg
     3721 tccatttggg acaggtaaat cttttattct aggtgcaatt gcgaatcagc tcaaatctaa
     3781 gaaggtacgt tcgacaatta tttatttacc ggaatttatt agaacattaa aaggtggctt
     3841 taaagatggt tcttttgaaa agaaattaca tcgcgtaaga gaagcaaaca ttttaatgct
     3901 tgatgatatt ggggctgaag aagtgactcc atgggtgaga gatgaggtaa ttggaccttt
     3961 gctacattat cgaatggttc atgaattacc aacattcttt agttctaatt ttgactatag
     4021 tgaattggaa catcatttag cgatgactcg tgatggtgaa gagaagacta aagcagcacg
     4081 tattattgaa cgtgtcaaat ctttgtcaac accatatttt ttatcaggag aaaatttcag
     4141 aaacaattga attttaaaat gattggtgta taatgaatac aaatctaaat cgtttaaatg
     4201 attgaagaca agatgatcta atcaatatta cacagaaagc cattgtttga tgagaatatg
     4261 gttaataaat tagatgatta ctacttcatt tatggtattt gtaatgaata cccggatcaa
     4321 gaccgttatc ttaagcagag atatttaata cataaattgt attaaataac tagggtggta
     4381 ccacgacgat ctcgtccctt gtataggggc gggatttttt gttttttcag acataaatgt
     4441 ttgttggtgt cataaattcc ctgtttattg ttaataggtt taatgttaaa acgatgattg
     4501 ttgttcaatt ttttaacgag gtcagataaa agtatttata aagcaaatag gagggtttaa
     4561 catggaacaa attaatattc aatttccaga tggtaataaa aaggcgtttg ataaaggtac
     4621 tactactgaa gatatagcac aatcaattag tcctggatta cgtaaaaaag ctgttgccgg
     4681 caaatttaac gggcaacttg tagatttaac aaaaccgctt gaaactgatg gatcaattga
     4741 aattgtgaca ccaggtagtg aagaagcgtt agaggtatta cgtcattcta ctgcacattt
     4801 aatggcacac gcgattaaaa ggttatatgg taatgttaaa tttggtgtag gtcctgtaat
     4861 agaaggtgga ttctactatg acttcgacat tgaccaaaac atctcatctg atgactttga
     4921 acaaattgaa aagacaatga aacaaatcgt taacgaaaat atgaaaatcg aacgaaaagt
     4981 ggtttcacga gatgaagcga aagagttatt cagcaatgat gaatacaaat tagaattaat
     5041 cgacgcgatt cctgaagatg aaaatgtaac attatatagt caaggtgatt ttactgattt
     5101 atgtcgtgga gttcacgttc catcaacagc taaaattaaa gagtttaaac tattatctac
     5161 agcaggtgca tactggcgtg gagatagtaa caacaaaatg ttacaacgta tatacggtac
     5221 tgctttcttt gataaaaaag aattaaaagc acatttacaa atgttagaag agcgtaaaga
     5281 acgtgatcat cgtaaaattg gtaaagagtt agaattattc acaaatagcc aattagttgg
     5341 tgctggtttg ccattatggt tacctaacgg tgcaacaatt agacgtgaaa ttgaacgtta
     5401 cattgttgat aaagaggtga gcatggggta tgaccacgtt tatacaccag tacttgctaa
     5461 tgttgattta tacaaaacat ctggtcactg ggatcactat caagaagata tgttcccacc
     5521 aatgcagtta gatgaaactg aatctatggt attacgtcca atgaactgtc cacatcatat
     5581 gatgatttat gcgaataaac cacattcata tcgtgaatta cctatccgta tcgctgagct
     5641 aggaacgatg catagatatg aagcaagtgg tgctgtatca ggattacaac gtgttcgtgg
     5701 tatgacttta aatgattcac atatctttgt tcgacctgat caaattaaag aagaattcaa
     5761 acgcgttgta aacatgatta ttgatgtgta taaagacttt ggtttcgagg attatagctt
     5821 tagattaagt tatagagacc ctgaagataa agaaaagtac tttgatgatg atgatatgtg
     5881 gaataaagct gaaaatatgc ttaaagaggc agcggatgag cttggcttat cgtacgaaga
     5941 agcgattggt gaagcggcat tctatggtcc gaaactagat gttcaagtta aaacagcgat
     6001 gggtaaagaa gagacattat caacagcaca acttgatttc ttattaccag aacgttttga
     6061 tttaacttat attggtcaag atggtgaaca tcatcgtcca gttgttattc atcgtggtgt
     6121 tgtatcaaca atggaacgat ttgtagcatt tttaactgaa gaaactaaag gtgcattccc
     6181 aacttggtta gcgccaaaac aagttcaaat cattccagtt aacgttgatt tacattatga
     6241 ttatgcgcgc caattacaag atgaattgaa atctcaaggc gttcgtgtaa gtattgatga
     6301 ccgtaatgaa aaaatgggtt ataaaattag agaagctcaa atgcaaaaaa taccttatca
     6361 aatcgtagtt ggggataagg aagttgaaaa taatcaagtg aatgtgcgtc aatatggatc
     6421 gcaagaccaa gaaacagttg aaaaagatga atttatctgg aatctagttg atgaaattcg
     6481 tttaaaaaaa catagataga cagttgtcgc aataaaatgc tttgaaattt ttattgcgta
     6541 tcaagtttca cagggttgat tatgcgtgat gaatcctgta tattacaagt tagttaaaat
     6601 attaaattga gttagaggtt gcatgtttaa ttagtaactt gtcagaagta tttatggtac
     6661 ataagttgaa caagtgaaag gtaaagatgc cgaaatagat ataaaccata aattatatct
     6721 attgggacag ttttcgaata ggaactgtac tgtcacagaa tgtgatgtgc taccttatat
     6781 agataattgc caaagtggtt gcatatctta aaggtatgta gccacttttt tacttttaat
     6841 atcactatgt tctgtaaaaa agggtatgaa agtgaataaa ggttatttat ttcttggcct
     6901 ctaaaacatg gaaagggagc ttatatgtca aaagttcaaa atgaaagtaa caatgttgtc
     6961 aaaaggggac ttaaagatcg tcatatttct atgattgcga ttgggggttg tattggtaca
     7021 ggtttatttg taacttctgg tggagcaatt catgatgcag gtgctttggg tgcattaata
     7081 ggatacgcaa ttatcggaat aatggtattt ttcttaatga cgtcacttgg cgaaatggct
     7141 acgtatttgc cagtatcagg ttcatttagt acatatgcta caagatttgt tgatccatct
     7201 ttagggtttg cgcttggttg gaactattgg tttaactggg tagtaactgt agcagcagat
     7261 attacgattg cagcacaagt cattcaatat tggacaccat tgcaaggcat acccgcttgg
     7321 gcatggagtg cgttgttctt agttataatt tttagtctga attcgttatc agttcgcgtc
     7381 tatggtgaaa gtgaatactg gttggcattg ataaaagtgg ttacagttat tgttttcatt
     7441 gcaattggtt tattaacgat tgtcggaatc atgggtggtc atgttgtagg attcgaaata
     7501 tttaataaag gtgaaggtcc tattcttggt ggcaacttag gaggaagttt attatcaatt
     7561 ctaggtgtat tcttaatcgc tggtttctca ttccaaggta ctgagttaat tggtattacg
     7621 gctggtgaat cagaaaatcc tgaacgtgct gtgccgaaag caattaaaca agtattctgg
     7681 agaattttat tattttacat tttagccatt tttgttatcg gtatgttaat tccttatgat
     7741 agtagtgcat taatgggtgg tagtgataat gtagcaacgt ctccattcac attagtgttt
     7801 aaaaatgctg gatttgcgtt tgcagcatca tttatgaatg cagtcatttt aacgtctgtg
     7861 ttatcagcag gtaactcagg tatgtatgct tcaactcgaa tgctatattc aatgagtaaa
     7921 gataaattag cgtttgaagc atttggtaaa acaaataaaa acggtgtgcc atatatgtcg
     7981 ttactcgtta cagcaatcat tgtagtgata attttcgtat tacaaagcct tacaaaaggt
     8041 gcatacgaat atatcgttgc agcaagtggt ttaactggtt ttattgcttg ggtaggtata
     8101 gcagtaagtc attatcgatt tagaagagca tttgataaac aaaattatga caaatctaaa
     8161 ttaaaatata ctgctaaatt attcccattc ggaccattat ttgctggctt tttatgtatt
     8221 gtagtaatta ttggtcaaga tgttgacttt attaaaacag gagattttaa ttttagtcgc
     8281 ttcttaatta cgtatatggg aatcccagta tttttagcat tctttattta tcataagctt
     8341 cgatataaaa cgaaaaaaat tccattagaa caagtagatt tacgtcaaga cgtgtcaatg
     8401 gatgaaataa aaggttaaaa taaaaataat tacagttata gtgttgactt aaatgtgaat
     8461 tgttgatatg attaacaacg ttgaatacga aacgaaccaa gtagaagcac ccgcttctca
     8521 cctgtagcga cgcataaagc agttggcagg ttaatcacat tacacataac tgtgtataag
     8581 gaagagcggg tattttatac ctgctcttcc tttttgcttg gtgcaaattt cgtaaaaatt
     8641 ttggaggtgt caaccatagc aaaagatcaa actcaaatca atgacaaaat tcgtgcaaaa
     8701 gaattacgtt taatcggtca agatggtgaa caaattggtg ttaaatcaaa gcgtgaagct
     8761 ttagaaatgg ctgaacgtgt agatttagac ttagtggtcg ttgcaccgaa tgcgaaacca
     8821 ccagttgcaa gaattatgga ttacggtaaa ttcaaattcg aacaacagaa aaaagaaaaa
     8881 gaaatgaaaa agaaacaaaa aattatcaat gttaaagaaa ttcgtttaag tccaacaatt
     8941 gaggaacatg atttccaaac taagttgaaa aacggacgta aattcttaac taaaggcgat
     9001 aaatgtaaag tatctattcg tttcagaggg cgtgccatta cgcataagga aattggtcaa
     9061 cgtgtgctag aaaaatatgc agatgaatgc aaagatatag caacagttga acaaaaacct
     9121 aaaatggacg ggcgtcaaat gtttatcatg ttagcgccaa cagctgaaaa ataattaaag
     9181 ctattacagg aggacttata ttatgccaaa aatgaaaact caccgcggag cagctaaacg
     9241 tgttaaaaga actgcttcag gtcaattaaa acgttcaaga gctttcacat ctcacttatt
     9301 cgcaaacaag agcactaaac aaaaacgtca attacgtaaa gctagattag tgtctaagag
     9361 cgatatgaaa cgtgtaaaac aattattagc atacaaaaaa taagaacaaa tacagaaatc
     9421 ggtaggaatt acctaaggag gaatttttta tgccacgagt taaaggtgga acagtaacaa
     9481 gagcgcgtcg taaaaaaacg attaaattag ctaaaggtta cttcggttca aaacatacat
     9541 tatacaaagt agctaagcaa caagtaatga agtcaggtca atatgctttc cgtgaccgtc
     9601 gtcaacgtaa acgtgacttc cgtaaattat ggattacacg tatcaacgca gcagctcgtc
     9661 aacatgaaat gagctactca cgtttaatga acggtttgaa aaaagctggt atcgacatta
     9721 accgtaaaat gttatcagaa atcgcaattt ctgacgaaaa agcatttgct caattagtaa
     9781 ctaaagctaa agatgcttta aaataataac acttataaaa aagagatggc attgtcatct
     9841 cttttttatt ttttaaaaag caaaatgcca catttaatcg ctaaaatatg acatatttgc
     9901 taaaatgcat ttaagagaac aaacattaaa gtgaggagtt ttatacgatg tctaaatttg
     9961 atgaacaaat cattgtagta cctagagaaa ttatttttaa taatgaaaag aatactttca
    10021 atgggttttt aaataagaat aaaccagaag gtcaaaatat ttttgatgcg ctaagtcaat
    10081 atgaagttaa gcgacgcggc gatatggaag aggatccatc atataaacaa ctcatttctt
    10141 attgtttact tgaaaatgag catggcgaga tattagtgta tgaacgatta tctggcggtg
    10201 gagaagctcg attgcatgga caatcttcaa taggtgtagg cggtcatatg aatgatgttc
    10261 caggagcaga atctattaac gaagtattga gagttaatgc acagagagaa ttagaagaag
    10321 aagtaggttt aagtgagcaa gattcacaaa atatggaata tatcggtttt attaatgacg
    10381 ataataatga agtgggcaag gtacatattg gtgttgtatt taaaatcact gtaagtacga
    10441 atgatgtaga agctaaagaa acagatactt tacgaataaa atgggttgaa aaaggcaaca
    10501 tagagtcata tgatgatttc gaaacgtgga gcgcattaat ccttcaagat ttataatcaa
    10561 acgaggtgac atatatgtca gatattattc agtttcctaa ctcttcgaaa aagttatata
    10621 aagatataaa acgtgctgaa caagaccaaa actatgacct gatgtatgaa tatattgtgc
    10681 aatatgaacg tcaatttgaa ctcactgaag aaatagcaat gatgaaatgc cgtatgttat
    10741 atgagacggg gtcatttctt gaattaagag aagaaacaat tgtcttattg aaaactggca
    10801 tacaacaata tgatgcattg atgatttatt acgtgaaaag tttgattggt ttgggacaat
    10861 attttgaagc ggtagaagta attcatcaaa ttattgatga agtcaaagat cacaaaacaa
    10921 gaatggcatt acatcctctt aaagaatttg ctaagtcaaa attaattgaa gatgaaaaac
    10981 gattgactca gtcattaact gattttgata cgttatcaat gagggaacag acgcacttga
    11041 ttttgaagtt aatagacaat ggtcattttc aatttcaaga aacggtatta tatatattaa
    11101 aatctaatac gtacagttat aacctcatta gtttaatgat tgagtattta aggtttgcaa
    11161 attgtacaca agaactgaca attgaaaagt atggtatgga tgtaactttt gtaccagcta
    11221 atttaaatgg gctagaacat acaacactga aagaaaaagt tatacctaac gttatggaaa
    11281 cattaaatga tggtgcatta catatcgctg aagaagcaca ccatattatg aataatcact
    11341 ctatcatgat ttatccaatt gatattgaaa ctttgtttga aacgaataaa tggattaatg
    11401 catacgaatg ttatttcaaa aatatgttag gacttaaatg tgaattacaa agtatcgatg
    11461 catttaattt tattcagcaa ttagatttga ataataatag ttaaattgtt gcaaaataat
    11521 actcagcatt ttaatatagt tttgacgaaa aaagttgatt tacaaatata taaacgttgt
    11581 gatttcaatg tttgtatagg gaatagacct atgatataat agggaagttg aaaaaataaa
    11641 ttagttaatc atggaggtat atatacatga cagcaacttg ggaaaaaaag gaaggtaacg
    11701 aaggtttatt aactgttact gttcctgcag aaaaagtaaa caaagcgtta gatcaagcat
    11761 tcaaaaaagt ggttaaacaa attaacgtac ctggattccg taaaggtaaa gtgccacgtc
    11821 caatttttga acaacgcttt ggtgtagaag cattatatca agatgctatc gacattttat
    11881 taccagatgc ttatggtgaa gcaattgacg aaactgatat taaaccagtt gcacaaccag
    11941 aagtaagtgt tactcaaatt gaaaaaggta aagatttcat ttttgaagca acagttacag
    12001 ttgagccaga agttaaatta ggagactata aaggtcttga aattgaaaaa caagaaactg
    12061 aattatctga tgatgagtta caagaagcga ttgaccacag cttaggacat ttagctgaaa
    12121 tggtagttaa agaagatggt gttgttgaaa atggcgacac agttaacatt gactttagtg
    12181 gttcagttga cggagaagaa ttcgaaggtg gacaagctga aggttacgat ttagaaatcg
    12241 gttcaggttc attcatacct ggtttcgaag agcaattaga aggtatgaaa gttgacgaag
    12301 aaaaagatgt tgtcgtaaca ttcccagaag aataccatgc tgaagaatta gctggtaaag
    12361 aagcaacttt caaaacaaaa gttaacgaaa ttaaatttaa agaagttcca gaattaacag
    12421 atgaaattgc taatgaatta gatgcagaag caaatacagt agacgagtac aaagaaaact
    12481 tacgtaaacg tttagctgaa caaaaagcta cagatgctga aaatgttgaa aaagaagaag
    12541 cgattacaaa agctactgat aatacaacaa tcgatattcc tgaagcaatg gttaatactg
    12601 aattagatcg tatggtgtct gaatttgcac aaagaattca acaacaaggt ttagatttac
    12661 aaacgtactt ccaaatctca ggtcaagatg aaactcaatt aagagagcaa atgaaagacg
    12721 atgcagaaca acgtgttaaa actaacttaa ctttaactgc gatcgctgaa gctgaaaaaa
    12781 tcgaagctac tgatgaagat atcgataaag aattagaaaa aatgagtaaa caatttaata
    12841 tctcagttga agatatcaaa aatactttag gtaatactga tatcattaaa aatgatgttc
    12901 gtatccaaaa agttatcgat ttattaagag ataacgcaaa gttcgttgaa ggaactaaag
    12961 aagattaatc ttcattaaat attaaattac aaaaatgagt agcagatgca tagcttatgt
    13021 atctgctact attctttaag caaaaagttt gtatgttaat atgttgcatt gtaatatcca
    13081 atctagtata gtctttaacg aataggggtg taaaaagaat gtttaaattc aatgaagatg
    13141 aagaaaattt gaaatgctct ttctgcggaa aagaccaaga tcaagtaaaa aaacttgtag
    13201 caggaagtgg tgtatatatt tgtaatgagt gtattgaatt atgctcagaa atcgtcgaag
    13261 aagaattagc tcaaaacact tctgaagcga tgacagaatt acctactcct aaagaaatta
    13321 tggatcattt aaacgaatat gttattggtc aagaaaaagc taaaaaatct ttagctgtag
    13381 ctgtttataa ccactataag cgtattcaac aattaggacc aaaagaagat gatgttgaat
    13441 tacaaaaaag taacattgca ttaattgggc caacaggtag tggtaaaaca ttattagctc
    13501 aaacattagc caagacgttg aatgtaccat ttgcaattgc agatgcgaca agtttaactg
    13561 aagctggtta tgtaggcgat gatgttgaaa atatcttgtt gagattaatt caagcagctg
    13621 actttgacat tgataaagcc gaaaaaggta ttatttatgt agatgaaatt gataaaattg
    13681 cacgtaaatc tgaaaacaca tctataacac gtgacgtttc aggtgaaggt gttcaacaag
    13741 cattgcttaa aatcttagaa ggtacgactg caagtgttcc gccacaaggt ggacgcaaac
    13801 atccaaacca agaaatgatt caaattgata caacaaatat cttatttatt cttggtggtg
    13861 cctttgatgg tattgaagaa gtgattaagc gccgtcttgg tgaaaaagtt attggtttct
    13921 caagcaatga agctgataaa tatgacgaac aagcattatt agcacaaatt cgcccagaag
    13981 atttgcaagc ctatggtttg attcctgaat ttatcggacg tgtgccaatt gtagctaatt
    14041 tagaaacatt agatgtaact gcgttgaaaa acatcttaac gcaacctaaa aatgcacttg
    14101 tgaaacaata tactaaaatg ctggaattag atgatgtgga tttagagttc actgaagaag
    14161 ctttatcagc aattagtgaa aaagcaattg aaagaaaaac aggtgcgcgt ggtttacgtt
    14221 caatcataga agaatcgtta atcgatatta tgtttgatgt gccttctaac gaaaatgtaa
    14281 cgaaggtagt tattacagca caaacaatta atgaagaaac tgaaccagaa ctatacgacg
    14341 cagaaggcaa tttaattaat aatagtaaaa catcagctta atcattgatg tgttataaaa
    14401 gtgaaaagct ccaatcaaag ttgacgctca aatagtttaa ctttgatcgg agctttttta
    14461 taatgtatac cgaaaggttc aaatattata taatggtaga gttgaaagag aatataaaaa
    14521 agtgaggata taaaatgaaa gttaatccta ataatataga attaatcatt agtgcagtaa
    14581 aagaagaaca atatccagaa acagaattgt ctgaagttgc actgagcggt cgatctaatg
    14641 taggtaagtc tacatttatc aatagtatga ttggcagaaa aaatatggca cgtacatcac
    14701 agcaacccgg caaaacgcaa acgttaaatt tttataatat agatgaacaa cttatttttg
    14761 tggatgttcc agggtatgga tatgctaaag taagtaaaac acaacgtgaa aaatttggga
    14821 aaatgattga ggaatatata actaagagag agaatttgca attagttatt caattagttg
    14881 atttaagaca tgatccaaca caagatgata tcttaatgta caattatttg aaacattttg
    14941 atattcctac tttagttata tgcactaaag aagacaaaat tccaaaaggt aaagttcaaa
    15001 agcatattaa aaatattaag acacaattag atatggaccc agacgataca attgtaagtt
    15061 attcatcaat tcaaaataat aaacaacaac aaatatggaa tttaattgaa ccgtatattt
    15121 catagttttt gtacgacaaa acttatacaa aaattttaaa aataatgtaa gcacgaaa
//
LOCUS       DAHHYJ010000058        14856 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000058 DAHHYJ010000000
VERSION     DAHHYJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.058"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..459)
                     /locus_tag="NBK22_001352"
     CDS             complement(<1..459)
                     /locus_tag="NBK22_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414543.1"
                     /translation="MNEQIIGSIYTLAGGVVLYSVKEIFRYFTDSNLQRKKINLEQIY
                     PIYLDCFKKAKKMIGAYIIPTEQHEFLDFFDIEVFNNLDKQSKKAYENVIGFRQMINL
                     SNRVKAMEDFKMSFNNEFSTNQIFFNPSFVMETIAIINEYQKDISYLKNII"
     gene            complement(618..767)
                     /locus_tag="NBK22_001353"
     CDS             complement(618..767)
                     /locus_tag="NBK22_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2414544.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            complement(764..970)
                     /locus_tag="NBK22_001354"
     CDS             complement(764..970)
                     /locus_tag="NBK22_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCD2414545.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKERY
                     EAQVKRGAVIKVGQLFENIRECGK"
     gene            complement(1007..1513)
                     /locus_tag="NBK22_001355"
     CDS             complement(1007..1513)
                     /locus_tag="NBK22_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HCD2414546.1"
                     /translation="MNNLTVDQLQELLQIQKEFDDRIPTLNLGDSKIAYVVEFFEWFN
                     TLETFKNWKKKPGKPLDVQLDELADMLAFGLSIANQQEVTNEKLEYGLSTLRKDGYLY
                     NESQSVWDFMSDVSNVGLEPLSAVIIPLDIAYNLYSIDQLIDAYKKKMKRNHERQDGT
                     ADAGKGYV"
     gene            complement(1528..1689)
                     /locus_tag="NBK22_001356"
     CDS             complement(1528..1689)
                     /locus_tag="NBK22_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414547.1"
                     /translation="MLEIIDQRDALLEEKYLNDDWWYELDYWLNKRKSENEQIDIDRV
                     LKFIEELKR"
     gene            complement(1973..2146)
                     /locus_tag="NBK22_001357"
     CDS             complement(1973..2146)
                     /locus_tag="NBK22_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414548.1"
                     /translation="MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIID
                     FITKPIYLIKGDE"
     gene            complement(2136..2387)
                     /locus_tag="NBK22_001358"
     CDS             complement(2136..2387)
                     /locus_tag="NBK22_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCD2414549.1"
                     /translation="MNNREQIEQSIISASAYNGNDTEGLLKEVEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQENEY"
     gene            complement(2402..2644)
                     /locus_tag="NBK22_001359"
     CDS             complement(2402..2644)
                     /locus_tag="NBK22_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCD2414550.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKTFDTAEELETYIKQHGLEYEEQKQLTLF"
     gene            complement(2648..3016)
                     /locus_tag="NBK22_001360"
     CDS             complement(2648..3016)
                     /locus_tag="NBK22_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414551.1"
                     /translation="MARKARIVTINDKPYRFSKFEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKQKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            complement(3029..3433)
                     /locus_tag="NBK22_001361"
     CDS             complement(3029..3433)
                     /locus_tag="NBK22_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCD2414552.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFRNTGRYAHTYMPTKYTEHKKYL
                     QNQMPKLNLENALKIELDFYFPLLKSWSKKKKSEMVGQYKVTKPDIDNLIKTVLDACN
                     GHVWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            complement(3442..3660)
                     /locus_tag="NBK22_001362"
     CDS             complement(3442..3660)
                     /locus_tag="NBK22_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414553.1"
                     /translation="MDAFDKYYLFDHYGNKMFSVTPHFKDGRHLVVGIKHTKFNGRRW
                     YLDDYELKTLIDNEQMELGHQTSLFEYI"
     gene            complement(3667..4551)
                     /locus_tag="NBK22_001363"
     CDS             complement(3667..4551)
                     /locus_tag="NBK22_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCD2414554.1"
                     /translation="MTGWIKLHRKLLDSPIFQNEKLFKVFAYCLMKASHKDHTQLVGR
                     RVVELEKGQFVFGRKRASEELRLKESTVRDYIKLLENLGTIVVKSDNKFSVITVVNWA
                     IYQSMEENSDSKNDNKSTTNQQQINNKWTTNQQQINTNKNVKNGDNVKNGENEKKKVT
                     AFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDQIVTASLKIAKDRNKVTWGYAKSIL
                     NTWLNANLKSIEQVRAFEKQQLESKKQNYKPFVKQSKEKTPKWLTDSTRETKTPEVDE
                     NLEKDREAFIKRLNSKWE"
     gene            complement(4581..5051)
                     /gene="ssb"
                     /locus_tag="NBK22_001364"
     CDS             complement(4581..5051)
                     /gene="ssb"
                     /locus_tag="NBK22_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD2414555.1"
                     /translation="MLNRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVVADSIQF
                     LEPKNTNDNQQDLYQQQAQQSRGQSQYPYNKPVKDNPFANANDPIEIDDDDLPF"
     gene            complement(5052..5669)
                     /locus_tag="NBK22_001365"
     CDS             complement(5052..5669)
                     /locus_tag="NBK22_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD2414556.1"
                     /translation="MKFERVQKHFKYKTRHIAGCLITHEHGDHAKYTKQFVDNGVISY
                     MTAGTQQAMNFESHRLCTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVL
                     YVTDTKYLKYKFNGITHMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIG
                     MLKANDLTRLEEIHLIHLSSQNSNAKYIKSEIQKVTGAPVYVGGL"
     gene            complement(5750..6670)
                     /locus_tag="NBK22_001366"
     CDS             complement(5750..6670)
                     /locus_tag="NBK22_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCD2414557.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLKLPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGETGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            complement(6672..8627)
                     /locus_tag="NBK22_001367"
     CDS             complement(6672..8627)
                     /locus_tag="NBK22_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD2414558.1"
                     /translation="MMMDIKINKLTISNFAGIKEESFNFDGKDTKIYGNNATGKTTTA
                     TALQWLLFDKGLDGSTKSFNPVPLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPK
                     YTINQKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLD
                     WKKRRSLLFEIAKPINDEDVIKTNNDFKKLNNILGDHEIETKKKILTDKIKQINKDIK
                     DIPIRINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQ
                     SELKRIEDNNSASNENKIYALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLE
                     NHKGLKSDLEKSKNQKFEHLDDNVCSCCGQQLPTEQVNEAREKALQKFNVKKSKELET
                     IQTSINHIISEGKKIKPIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVT
                     QTDEYKAVMLEINEINQKRSNIRKTIQDNVSGIDDKISELTQEKSEIEVSRSIEKSNK
                     HLDDVISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFN
                     TLVNGELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESV
                     TELIKTESQQIQLIVNEQDKKLRMETI"
     gene            complement(8624..8887)
                     /locus_tag="NBK22_001368"
     CDS             complement(8624..8887)
                     /locus_tag="NBK22_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414559.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            complement(8896..9156)
                     /locus_tag="NBK22_001369"
     CDS             complement(8896..9156)
                     /locus_tag="NBK22_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCD2414560.1"
                     /translation="MYYKIGDVCQKVINVDGFDFKLAVKKQDYSILVNVLDLEDRFID
                     GINITDENDLYTALDILNQSIYEWIEENTDERDRLINLVMRW"
     gene            complement(9249..9410)
                     /locus_tag="NBK22_001370"
     CDS             complement(9249..9410)
                     /locus_tag="NBK22_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCD2414561.1"
                     /translation="MSNIYKSYLVAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            complement(9407..9727)
                     /locus_tag="NBK22_001371"
     CDS             complement(9407..9727)
                     /locus_tag="NBK22_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCD2414562.1"
                     /translation="MPHILNVTVPIPETHVLITKDEYDELIGYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            9786..10418
                     /locus_tag="NBK22_001372"
     CDS             9786..10418
                     /locus_tag="NBK22_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414563.1"
                     /translation="MYIDPLKNVRFSINNVISNVEISKSMAIKQSLKPKYQLDIINRN
                     NINLFSDFKVDFHLNNLIEMNFNLRNSFSSLTFQRNLFSEETIKSFKELYRFDDEIVL
                     QAQQTIRDFYINPTAISTLAEAINSTYPINEQSTYKRHDEFVKRIENDFPHPFKKLIR
                     WSNGIAAGADIQIFVTNYINENDLHIQNSLIVAIVCLLSFLSTYCSHSKK"
     gene            complement(10433..10573)
                     /locus_tag="NBK22_001373"
     CDS             complement(10433..10573)
                     /locus_tag="NBK22_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414564.1"
                     /translation="MLQKFRIAKEKNKLKLKLLKHASYCLERSNNPELLRAVAELLKK
                     VN"
     gene            complement(10604..10801)
                     /locus_tag="NBK22_001374"
     CDS             complement(10604..10801)
                     /locus_tag="NBK22_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414565.1"
                     /translation="MQAQNKKVIYYYYDEEGNRRPVNIQYNDGYDLMIDPRFIEMTLE
                     RHPHLKNNFYGLIDGKEFKLD"
     gene            complement(10817..11566)
                     /locus_tag="NBK22_001375"
     CDS             complement(10817..11566)
                     /locus_tag="NBK22_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCD2414566.1"
                     /translation="MQALKTKSNIGEMFNIQEKENGEIAISARELYKALEVKKRFSAW
                     AEINLKHFKENRDFTSVLTSTVVNNGAVRQLEDYALTLDVAKHVAMMSGTEKGFDFRE
                     YFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVGE
                     LAKIIKQNGINIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITHS
                     DGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            11623..12162
                     /locus_tag="NBK22_001376"
     CDS             11623..12162
                     /locus_tag="NBK22_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414567.1"
                     /translation="MDIIAICIAIFSFLLTALKYYLDYMKDSLNIDVIPTRSFNYLVD
                     DKSSYNDITFINFTKFPISVIDVEFDIKNKVNEQKTFKPIRYKDKNYSIPFTLGPYES
                     VECTFLLEEYPVIWEWDVTIKVTTNKGIYIKPVIIESRTEHRESEPQVTELTSANKVS
                     ALSNPKDGFLKKFLYHLKP"
     gene            complement(12186..12446)
                     /locus_tag="NBK22_001377"
     CDS             complement(12186..12446)
                     /locus_tag="NBK22_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2414568.1"
                     /translation="MEQITLTKEECVEQCINKDLKLLDYRVQQILEGVLSESTTYGDA
                     RNKLETLKIIAESHFKTEHASVIYKLALKKLDEKINATPIKE"
     gene            complement(12494..12706)
                     /locus_tag="NBK22_001378"
     CDS             complement(12494..12706)
                     /locus_tag="NBK22_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2414569.1"
                     /translation="MTTSVADKPYLKIKSLIALKGTNQKEVAKAIGMSRSLLSIKINR
                     INGRDFTTSEAKKLADHLNVKVDDFF"
     gene            12858..13589
                     /locus_tag="NBK22_001379"
     CDS             12858..13589
                     /locus_tag="NBK22_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2414570.1"
                     /translation="MGIGEGLKKLRKNKNMTMEQLATDLNNKYPDLMKLTKGKISKWE
                     NEKEEPRLSTAKILAEYFNVKINDLYSESNTTYKDDNDITSIYNKLTPPRQSNVLKYA
                     TNQLEEQNNDSDNLVDFNSYIQEKSEVDIYGCASAGIGERLYNEPISKEFVRGYVPAH
                     DIALKVNGDSMEPLFKNGQIIFIEKSHTIKDGQIGVFIINGDAYVKKVYVEDNRLTLV
                     SLNKKYKDLHFYDNESVRLVGKVIL"
     gene            13601..13756
                     /locus_tag="NBK22_001380"
     CDS             13601..13756
                     /locus_tag="NBK22_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414571.1"
                     /translation="MNLKEVDINIEEWEMVEIPFYTEEELTYRLKNDLPITKSEFEEQ
                     ESKNEFL"
     gene            13743..13928
                     /locus_tag="NBK22_001381"
     CDS             13743..13928
                     /locus_tag="NBK22_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414572.1"
                     /translation="MNSYKEIEHLHINTGGKELTQEQIEEAKSFIDSQGFKDMIREAK
                     ESRQRVMESKITDRTKM"
     gene            14128..14295
                     /locus_tag="NBK22_001382"
     CDS             14128..14295
                     /locus_tag="NBK22_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414573.1"
                     /translation="MKITNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLN"
     gene            14435..>14856
                     /locus_tag="NBK22_001383"
     CDS             14435..>14856
                     /locus_tag="NBK22_001383"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD2414574.1"
                     /translation="MEEKRYKVYQRGEIIYADFGKGVGHEFSYKHFCIVINKKDNKNN
                     GKVTVIPLTSKSKHNAFVNIHLIKPFLNTLKQSIAKHEKKIESIENIINQNPNDLKVI
                     VYARFLCDSLKNEMKQYDFVENKLKNLVDKSSYAKTQD"
BASE COUNT         4326 a         2613 c         2172 g         5745 t
ORIGIN      
        1 aattatattt tttaaataag atatatcttt ttgatattca tttataatag caattgtttc
       61 cataacaaaa gaaggattaa aaaaaatctg atttgtacta aattcattgt tgaaactcat
      121 cttaaaatct tccattgcct taactctatt tgataaatta atcatttgtc taaatccaat
      181 aacattttca tacgcttttt tactttgctt atctaaatta ttaaagactt caatatcaaa
      241 aaaatctaaa aattcatgct gttctgttgg aataatataa gctccaatca tctttttagc
      301 ctttttaaaa caatctaaat atatcggata tatttgttct aaattgattt ttttacgttg
      361 taagttagaa tctgtaaaat acctaaaaat ctctttaact gaataaagca caacacctcc
      421 tgctaaagta tatatgcttc ctattatttg ctcgttcatt tttatctacc tctttataat
      481 attttctgaa aaggaatcta taattttata cataccaaaa attcctaatg catttattat
      541 cactccatca tcaataatat atatggatat taagaaagca aacagcaaaa cgatcaaatc
      601 ataaataaat attctcatta ttcactcacc tccgctcgaa agacgtaatc actcggcgcc
      661 tctacatcat cattagccgt catcataata tatacttgct cagttacata cttacctaac
      721 tcgtacattg ctagtaggaa taataatctt agtatttgct taatcatttc ccacactccc
      781 ttatattttc aaacaactga cccactttaa taactgcacc tcttttaact tgcgcctcgt
      841 acctctcttt cgcttcttct ttactctctg cctcaacaac tgtaaacctt tgattgctct
      901 tagctttagt tatgtgtgta tgcttgcgtc ctgttgaatc tttgaatgtt gtgactaggt
      961 attgtgtcac ttccccaaaa cctccttgac tcgatctaat atgtctttac acgtatcctt
     1021 ttcctgcgtc tgctgttcca tcttgtcttt catgattcct tttcattttc tttttgtacg
     1081 cgtcaatgag ttggtcgata gaatataagt tgtaagcaat atctagtggt ataataactg
     1141 cacttaaagg ttctaaacca acgtttgata catctgacat aaagtcccaa acggattgag
     1201 attcattgta aagataccca tcttttctaa gagtgcttaa tccatattct aatttttcgt
     1261 tcgttacctc ttgttgattt gcaatactca atccaaacgc caacatgtca gctaattcat
     1321 ctaactgtac gtctaacggc ttacctggtt tcttcttcca gttcttaaac gtttccaatg
     1381 tattaaacca ttcaaagaat tcaactacat atgcaatctt gctatctcct aagttcagcg
     1441 tcggtattct atcgtcgaac tccttttgta tttgtaataa ctcttgtaat tgatctactg
     1501 ttaaattatt catttattcg ttatctccta tcgttttaat tcctcaataa atttaagcac
     1561 tctatcaata tcaatctgtt cattttctga cttgcgttta ttcaaccaat aatctaactc
     1621 gtaccaccag tcgtcgttta aatacttttc ttctagcaat gcatcacgtt ggtcgatgat
     1681 ttcaagcatt tactcgtcct ccaaaaattc aaaatatctt tcaatctcat gctcgttagc
     1741 aaagtaatat ctatgcgtct tatgagattt aggattaatc caaataatgt aaataggcat
     1801 cctttgaaat gataaaaatc aagttaataa cacttcttca ctaaaaattt caaagaaacc
     1861 taattctttt tgaacctctt tcacattatc ttttgttacg tataccgact ttaagttttt
     1921 atttttgata actgggtgcg gcaccacatt tttttaatcg tttccattcc actcactcgt
     1981 cccccttaat tagataaatt ggtttagtaa taaaatctat aatgctaata actgaatcat
     2041 cagacagttt ataatgtgta tctctaatat ctccgaccaa ttgcacaatc tctagacttt
     2101 cgtttgtttc atggttatat actttatctc ctacactaat actcattttc ctgctcctcc
     2161 tcatatttat agaccacttg ccccgtcata atccctactg cttcatcaag accaatatct
     2221 tctttgagtg catcttgcat agcattaggt aaaccctcaa gtatttcatc aaacgcttgc
     2281 gctttcttat acacgtcttc aacctctttt agtaatccct ctgtgtcatt accgttatag
     2341 gcactagcac tgataattga ttgttcaatt tgttcgcggt tattcatcat ttccatctcc
     2401 tctaaaataa agttagttgc ttctgctcct cgtattccaa accatgttgc tttatatatg
     2461 tttcgagctc ttctgctgta tcaaatgtct ttttcacgcc ttgccaacct ggtacgatat
     2521 gcccatgaaa gtaataagtg ccgtttacta catgaatatg tgccactcgt tcgttatcct
     2581 gatacagata tctcttagat ccaaagaatt gatttaggta ttctttgcgt gcgttatctg
     2641 tcatgatcta cttcttaact ttcacgaata tgtcgttttc catcaggtag cacgcataac
     2701 gtcctcttgg atgtttctgt ggtacattaa acaaatgtgg cttctttctt cttagctcag
     2761 cctctttctt ttgctttctt tccaatttgc gttcgagtct agcttgttcc agtctttcta
     2821 ttgttttctt ttctctgtac tcgcttaaac gcgtaccttc tggtgcgtcc attgcttcat
     2881 gtagttccca accgtctttt actctcttag aaaccattcc agcggttata ccgtgacttt
     2941 ctattaattc catttcaaat ttactgaacc tataaggttt atcgtttatt gttacaattc
     3001 ttgcttttct cgccatttta tccacctctt atatttcttc tattcgtatg attattttgg
     3061 gctcaattcc ataacgcttt gagctagtta tttctgtaat ttggttatcg tctttccata
     3121 catggccatt acaagcatct aataccgttt taattaagtt gtcgatatcc ggcttagtca
     3181 ctttatactg cccaaccatt tcgcttttct ttttcttcga ccatgattta agcaatggaa
     3241 agtaaaagtc taattcgatt tttaatgcat tttctagatt tagctttggc atttgatttt
     3301 gtaaatattt tttatgttct gtatattttg taggcatata tgtgtgtgca tatctacctg
     3361 tattacgaaa gcgtggacga ggcgacccca tcggcgcatt aaacacttca ttaaatttaa
     3421 tttctatttc catgtaatcc ctcatatata ttcaaataag cttgtttggt gtcctaactc
     3481 catttgttca ttatcaataa gtgtttttaa ttcataatca tctaagtacc aacgtcgacc
     3541 attgaatttt gtatgtttta ttccaacaac taaatgccgt ccatctttaa aatgtggtgt
     3601 aactgaaaac attttgttgc cgtaatgatc aaatagatag tatttatcaa atgcatccat
     3661 tttcaatcac tcccatttgc tatttagacg cttaataaaa gcttctctgt ctttctcaag
     3721 gttttcatct acttccggcg ttttcgtttc tctcgtgctg tctgtgagcc atttgggtgt
     3781 tttttctttt gattgtttaa cgaaaggttt ataattttgt tttttgcttt caagttgttg
     3841 cttttcaaat gcacgtactt gttcaataga tttcaagttt gcattaagcc atgtattcaa
     3901 aatgctttta gcatatcccc aagtaacttt atttctgtct ttagcgattt taagtgatgc
     3961 ggtaactatt tgatctgaat cattttcaaa tgaatcaaga taataattta aatcgtctaa
     4021 attgtaagga gttatgaaac cgaatccgtt atcttggaag aagtcgaagg cggttacctt
     4081 cttcttctca ttctcaccat tctttacatt atccccattc tttacattct tgtttgtgtt
     4141 gatttgttgt tgatttgttg tccatttgtt gttgatttgt tgttgatttg ttgttgattt
     4201 gttgtcgttt ttgctgtcgg aattttcttc catactttga taaatcgccc aattgacaac
     4261 ggttataaca gaaaatttgt tgtcggactt tacgacgata gttccaaggt tttctaaaag
     4321 ctttatgtag tctcttactg tggattcttt gagacgtaac tcttcgcttg ctcgctttct
     4381 cccgaacaca aattgacctt tttctaattc aacaactcgt ctgccaacaa gctgtgtatg
     4441 atccttatga ctagccttca taagacaata tgcaaatact ttgaataact tttcgttctg
     4501 aaaaataggc gaatctaata gttttctatg aagttttatc caaccagtca tatacacacc
     4561 tcactttcaa accggttaaa ttagaatggt aaatcatcgt catctatttc aataggatca
     4621 ttcgcatttg cgaacggatt atcttttact ggtttgttat atggatattg agactgtcca
     4681 cgtgattgtt gcgcttgttg ttggtataaa tcttgttgat tatcatttgt gttcttcggt
     4741 tctaaaaatt gaatactatc ggcaacaact tccgtaacgt atacacgttg accttcctta
     4801 ttttcatagt tccgcgtttg taacctacca tctacgcccg ccaacgatcc tttagatagg
     4861 tatttattaa cgttctctgc ttgtttttta aatacgatga tattaataaa gtctgcctcg
     4921 cgctctcctt gtgcatttgt aaatgtgcgg ttaactgcta atgtgaatga tgctacattt
     4981 acaccacttt gagtggttct taattctggg tctctagtta aacgaccaac taatattgtt
     5041 ctgtttagca tttataaacc tccaacataa acgggcgcgc ccgtcacttt ttgtatttca
     5101 cttttaatgt attttgcatt tgaattttga ctacttaaat gaattaaatg tatttcttcg
     5161 agtctagtta aatcatttgc ttttaacatt ccgatagcat gttctaagct aaaatgagac
     5221 tccataattc tgtttgctaa tgtgctgtgc acactgccgt tttttatgtt ttcttgcatt
     5281 tgttcataga tataattaac ttctagcatc atgtgcgtaa tgccattaaa tttgtatttc
     5341 aaatactttg tatcagtaac atacaggacc ttataaccta gtgtgctttg taataagaaa
     5401 gccacaggct cgttagcatc atgttcgatg tcaaacggta gaattgacca tgtgcctatt
     5461 cgcagctctt gctttgcctt aatcgtgcat aagcgatgac tttcaaaatt catagcttgt
     5521 tgtgttccag cagtcatata gctgattaca ccattgtcga caaactgctt tgtgtacttt
     5581 gcatgatcac catgttcgtg tgtgataaga caccctgcta tatgtcttgt tttatatttg
     5641 aaatgctttt gaacacgttc aaattttata cctgcctcaa gtagtaacgt agtacgtcca
     5701 tcatttaaga cgtagcagtt accacttgaa ccagttgcta ttgtttcaat taaaatggct
     5761 cttcttcgct ttctttttct gttgcaggtt cttttatttc ttcaaagtca gatacatcaa
     5821 taggcttatc attttctaat tctgtgtatt gtgcttcttc gagaactggt tgttcaaagt
     5881 ccaattgttc ttgatttgca ttttcttcaa cttctgcgtc taacacttct ttgcgttgac
     5941 gttgttcgga ttcttgtgcg tatttgaaaa gattgctatc tgttgatgtg ttgatataac
     6001 gtttagcagc tctattgata actgtttttt tagccatttc ttctttgaaa ttattatgtg
     6061 ttttagaatt ttgtaatgct ttttcatctt taatcattga tgactgcatc catgcttgtt
     6121 taatttgttc aatagtcatg acttcaatat agttatctcg tccatcatta aatacgattg
     6181 tgcagtacgc accgataatg ttttctttgt cgatgttaaa gaagtcttgt tcgtgtttaa
     6241 tcgctttgat acgtcctgtt tctcccattt cttgcttgaa tgtatcgcct ttataaatca
     6301 cttgagcaac aacatcttga gcacctgcat cacgttttaa catcattaca ttaccgtgat
     6361 agctacgttg taactgcatt ttgttgccgt aaggaataaa gtagcattga tttttagctg
     6421 gatttaaacc ttgcgttacc atgtctaata aggcatttgc tttgcttgta tcgttacaac
     6481 tcattaattt gttatcttgg ctgatttgta accatgcttg tttcatggca ttacttggtg
     6541 agtaatcatt tggcaatttc aaattgcctt gtgactctaa aactctcact ttgtttaata
     6601 cgttgtcaga tacgttcttt tcttgtacta attgttgttc aatagtttgt aatttattat
     6661 tttcagtcat tttatatagt ctccattctt aattttttat cttgttcatt tactatcaat
     6721 tgaatttgtt gtgattctgt tttgataagc tctgttactg attcagcatt atcaataaat
     6781 attggcgctg taactttaaa atgttttgac agtgtattga tgatatctaa gccaacatta
     6841 attcttgagg cgttatttaa accgctgtcg tattcgacgc cgttaaccgt tgtggaacat
     6901 gtttcttcta attcgccgtt aactaaggta ttgaataact taaattcagc aatctcaaat
     6961 tcattattga tattttcagt aagcattttg acttttgttg ttgtaaattc ttttaagata
     7021 taaaggtcat gtgaatactt ttctttttca tccaataatc tatcttcttc atttcttaat
     7081 tcagaaataa catcatctag atgtttattt gatttttcga ttgatcttga cacttcaatt
     7141 tctgattttt cttgagtaag ttcgcttatt ttgtcatcta ttcctgaaac gttatcttga
     7201 atagttttcc taatgttcga gcgtttttga ttaatctcgt ttatctctaa cattactgct
     7261 ttgtattcgt cagtttgtgt aacgtcaaca tgagtcgttt tcaacttatt aattttgttt
     7321 tgtattcttg ctgaacgctc ttctgcttcg ttgattttaa tttgtagatt attgttgtca
     7381 tcctctaact tctcgatgat tggctttatt ttctttcctt ctgaaataat gtgattgata
     7441 gatgtttgta ttgtttctaa ttctttcgat ttttttacat tgaatttctg taaagctttt
     7501 tctcttgcct cattcacttg ttcagttggt aactgttgac cgcaacagct acatacattg
     7561 tcatcaagat gttcaaattt ttgattttta gatttttcta aatcactttt tagtcctttg
     7621 tgattttcca ataattgatt acgtctattt tcttcatgcg taatttgttg tttgttttgc
     7681 tttaatctcg ttttaaggtt cgcaaccgtt ccattttcaa cgtgtaactc atttgttaaa
     7741 gcataaattt tgttctcatt acttgcgctg ttattgtctt ctatgcgttt caattctgat
     7801 tgtttatcag ctaattgatt acgcaaatta atttcttcct taccgttttg aatatctata
     7861 cgctcatttt caagttgctc aatttcttgt ttgataattg cgtatctatc gttatcgaat
     7921 tctggtacat cctgcttatt ttgttgtgtt tgattaatac gtatcggaat atctttgatg
     7981 tctttgttaa tctgctttat cttgtcagta agaatctttt tctttgtttc aatttcatga
     8041 tcaccaagaa tattatttaa ttttttaaag tcgttatttg ttttaatgac atcctcatca
     8101 ttaattggtt ttgcaatttc aaataacaaa cttcttcgtt tcttccaatc gagtaaatta
     8161 aatgcttggg gatttgtaat caacttgaat acatcttcat caattagttc atcaatacga
     8221 gctttataat cctttacttt tattgattca tcattgatat attgtttctt tgttctactt
     8281 cgtgagtatt ccttgcgatt cgtcttttga tttattgtgt atttaggatg tgactctttt
     8341 ttgaaagtcg ttatttttcc gtcgatttca aattctgcga aaacagtcgg aattaactca
     8401 taattttctt cgtttttttc gtttaaaggt acagggttaa atgatttggt tgatccgtcc
     8461 aaacctttat cgaaaagcag ccattgtaat gcggttgcag tcgtagtctt accagtcgca
     8521 ttattgccgt atatttttgt gtctttaccg tcaaagttaa agctttcttc tttgattcca
     8581 gcaaagtttg atattgttag cttatttatt tttatatcca tcatcatgct ccttttttaa
     8641 tctttcggtg acctcttaac acctcgataa ttaaattttt tattcgttcg tggctgtccg
     8701 gattgatttc atgtatctgt acaagcttat tgttcgtttt gtaactgtcg tgatagtgca
     8761 agaaattaat cgataagtat ccgtgatgat tacgttcaat ttccaataat gctcgttggt
     8821 ttgacaaagt atattcgtcg aataacgtct taaaaatatt caatatattt ctttctgtat
     8881 ctctcatgct tatacctacc atctcatgac taagttaatt agcctgtctc tttcgtctgt
     8941 gttctcttca atccattcat aaatagattg atttaatatg tctaatgctg tgtatagatc
     9001 attctcatct gttatattta taccgtcgat aaatctatct tctaaatcta agacattcac
     9061 tagaatgctg taatcttgtt tcttaactgc taatttaaaa tcgaatccgt ctacattaat
     9121 tactttttga catacatcgc caattttgta gtacattgtt gacacttcct ttatttcgtt
     9181 ttatattgaa tatgcattaa ttttctaatt gtttagactg ttactcattg ccgtgagtaa
     9241 cagttttttt attcttcata aaagtattcc ttataaaata tgaatgttgc gatacttgcg
     9301 aatcccgcaa tcgaccatgc agtagtgaag tatagaaacg gcataagtac aattgctaag
     9361 actgtaaagc acagtactgc tactaggtag cttttataaa tgttgctcat tttattctct
     9421 ccttatatat ttcattgaaa tgctcatcga cgaatttatt catctttctt gcgttaaatc
     9481 tccagcgatt aaaattctca tcagggtaat gcacaattcc ttgcgctctt agttcttttt
     9541 caaatctagg atgaaatagt aatctgtcct tgatagtctc atcagatgca atttttaatt
     9601 tcttctttaa gtcactcatg ttccatacag ggtctaatga ataaccaatt agctcatcat
     9661 attcatcttt tgtgataagt acatgtgttt caggtattgg aactgttacg tttaaaatat
     9721 gtggcatttc tatctttcct ttcgtgtata atgttgttat ctcctagtga aaggaggtga
     9781 tattggtgta tattgatcct ttaaaaaatg ttcgtttttc tattaataac gtaattagta
     9841 atgttgaaat ttctaaaagt atggcaatta aacaatcttt aaaacctaag taccaattag
     9901 atataattaa tagaaacaac ataaatttat tttctgactt caaagtagac tttcatctaa
     9961 acaacttaat tgaaatgaat tttaatttgc gtaattcttt ttcatctcta acatttcaaa
    10021 gaaatttatt ttctgaagaa acgataaaat cttttaagga actctatagg tttgatgatg
    10081 agattgtact tcaagcacaa cagaccatta gagattttta tatcaatcca actgctatct
    10141 ctactttggc tgaagccatc aattcgacct atccaataaa tgagcaaagt acctacaaga
    10201 gacacgatga atttgtcaaa cgtatcgaaa atgattttcc acatcctttc aaaaagttaa
    10261 taagatggtc taatggcatt gcagcaggtg ctgacattca aatctttgta acaaactata
    10321 taaacgagaa cgatttacat attcaaaatt cattgatagt tgctatagtt tgtttattaa
    10381 gttttttatc gacctattgt tcacattcta aaaagtaata ataaggccta atttagttaa
    10441 ccttctttaa caactctgca actgctcgca acaattcagg gttgttactt ctttctaaac
    10501 agtaactagc atgctttagt aatttgagtt ttaatttatt tttttctttc gcgattctaa
    10561 atttttgtaa catttgttat gcctcctttg catttccaaa aatttaatct aacttaaatt
    10621 cttttccatc tattaatcca taaaagttat tttttaaatg cggatgtctt tcaagcgtca
    10681 tttcaataaa acgcgggtct atcattaagt cgtagccatc gttgtattga atattaacgg
    10741 gtcgtctatt accttcttcg tcatagtagt aatagatgac ttttttgttt tgagcttgca
    10801 ttgttcgttc ctcctattaa gatgtttgtt tttctcctaa aaacttatta acaaagtatt
    10861 gttgtccttt gcctgttact tttggcgtct tactaattga tgtgtgaccg tccgaatgtg
    10921 tgattgatgt ttctttaatt tcgaataact cacgttccat tgaatactgt gtaggcatgt
    10981 tataatccac acccttgcgt ttaataagga atccgttttg acgtaaccac tcaaacaatc
    11041 tgcgttgccc gatgtttata ccgttttgtt taatgatctt tgctaactct ccaactaaaa
    11101 ttgatgtctt agtagtagct actgcatctg caaatacaat ttttggttta tcacgttcaa
    11161 tctttgtttc taattgattg attgtgttgt tagcaatttt taaagcacgt tgcataatca
    11221 tttctggact gttccatgct ttctctactt ggatgaaata ctctctaaaa tcaaaacctt
    11281 tttctgtacc tgacatcatc gcaacatgtt tagctacatc aagtgttaaa gcataatctt
    11341 ctagttgtct tacagctccg ttattaacaa ccgtacttgt aagtacactt gtaaaatccc
    11401 tattttcttt gaaatgcttc aagttaattt ctgcccaagc gctaaaacgc tttttaactt
    11461 ccaaagcttt atataactct cttgcactta ttgcgatttc tccattttct ttttcttgta
    11521 tgttgaacat ttcgccgatg ttcgattttg tttttaatgc ttgcatattg tttatgctcc
    11581 tttcgtgtat aatgttgtta tcaacctaag gaggtgataa gtatggacat aatcgcgatt
    11641 tgtatcgcaa tttttagttt cttactgact gcacttaaat attatttaga ctatatgaaa
    11701 gattctctta acatcgatgt tatacctacc agaagcttta attacttggt cgatgacaaa
    11761 tcaagttaca acgatataac atttattaat ttcacaaagt ttcccatttc tgttattgac
    11821 gttgaatttg atattaaaaa taaagtaaat gaacaaaaaa cgttcaaacc tatacgatat
    11881 aaagataaaa actactccat tccatttact ttaggacctt atgaaagtgt agaatgtact
    11941 tttttgctcg aagaatatcc agtgatatgg gaatgggatg tgactatcaa agtcactacc
    12001 aacaaaggaa tctatataaa gcctgttatc atagaatcgc ggacagaaca ccgagaatca
    12061 gagccacaag tgacagagtt aacatcagca aataaggtaa gtgctctttc caaccccaag
    12121 gatggttttt taaagaagtt tttatatcat ttaaaacctt aaacatttaa aatcctccct
    12181 ttccgtcact ctttaattgg agtggcgttg attttttcgt ctaacttttt caatgctaat
    12241 ttgtaaataa ctgaagcatg ttcggtttta aaatgagatt cagcaataat tttcaatgtt
    12301 tctaatttat ttcttgcatc accgtatgtg gtactttctg atagaacacc ttctaaaatt
    12361 tgttgaactc gataatctaa aagttttaag tctttattga tgcattgttc gacacactct
    12421 tctttggtta atgtgatttg ttccatagtg tcctccttat ttttatttag ttgtcacttt
    12481 cgaaacttaa agtttaaaaa aaatcatcaa ctttaacatt taaatgatct gctaattttt
    12541 tagcttctga agttgtaaaa tctctgccat taattcgatt tatctttata ctcaataaac
    12601 ttctactcat tccgattgct ttagcaactt ctttttggtt agttccttta agtgcaatca
    12661 agctttttat ttttaagtat ggtttatctg ctacactagt tgtcattgaa ccccctcctt
    12721 ttgtttcgtt tgtaacaact tgatttaaga atacatcata aaagtttcga tgtaaacaac
    12781 ttttgcaata atattttcct tgcgtttcgt tttcgaaact tttataatga aattatctta
    12841 tataaggagg gtttcgtatg ggtattggtg aaggtttaaa gaagctaaga aaaaataaaa
    12901 atatgactat ggaacaatta gcaactgatc ttaataataa atatcccgac ttaatgaaat
    12961 taacgaaagg caagatatca aaatgggaaa atgaaaagga agaacctcga ttatcaactg
    13021 ccaaaatttt ggctgagtac ttcaatgtga agattaatga tttgtatagt gaatcaaata
    13081 ctacatacaa agacgataac gacatcactt ccatatacaa taaactcaca cctccaagac
    13141 aaagcaatgt actaaaatat gcgactaatc aattagaaga gcaaaataat gacagtgata
    13201 atctggtaga tttcaattct tacattcaag aaaaatccga agtggatata tatggttgtg
    13261 cgtctgctgg tattggtgaa agattatata acgagcctat ttcaaaagaa ttcgtaagag
    13321 gttatgtccc cgcacatgat atagctttaa aagtaaatgg agactcaatg gagccgttat
    13381 ttaaaaatgg acaaattata ttcattgaaa aatctcacac tatcaaagat ggacaaatag
    13441 gcgtctttat tataaatgga gatgcttacg taaagaaggt ttatgtagaa gataacagat
    13501 taacgttggt ttctttaaat aaaaagtata aagatttaca tttttatgat aatgaaagtg
    13561 tgaggttagt tggaaaagtt attttatagg aggtagtaaa atgaatttaa aagaagttga
    13621 cattaacatt gaagagtggg aaatggttga aatccccttt tatacagaag aagaactgac
    13681 ttatagattg aaaaacgatt tacctataac taaaagtgag tttgaagaac aggagtctaa
    13741 aaatgaattc ttataaagaa attgaacact tacacattaa caccggtggt aaagagttaa
    13801 ctcaagagca aatagaagaa gctaaatctt ttatagacag tcaaggattt aaagatatga
    13861 ttcgagaagc taaagagtca cgtcaaagag ttatggagtc taaaattact gatagaacta
    13921 aaatgtgatt aataacgtat atttagcgct ttaatataaa tataaacaaa ggagaaattg
    13981 acatgaaaaa agcaatctta actttaagtc ttatatttat tacctactac ctcactttta
    14041 aatatatgtg gattaaagaa ttgaagtatt aattatgctt atttaaaaaa gacgtctatt
    14101 tcagcagtgt ttgaaaggaa gtttataatg aaaataacta attgcaaaat aaaaaaagaa
    14161 actatagtat atgaagtttt aactagtggt aatcaaccat tcacttatga gttacctaaa
    14221 gatttatcgt cacataatgc gcgtaaatac ttggaattta tttcacaaaa aatagatggc
    14281 gataagttaa attaattcaa agaataaagt aacttcataa agagtacgaa gaaaacgatc
    14341 taatgaccga acttattctt gaatatttag taaaaaagta tgttgaagaa gaatatagga
    14401 aataaacgcc tatatggcgt gaggaggatg agggatggaa gagaaacgat ataaagttta
    14461 tcaaagaggc gaaataattt atgcagattt tggtaaaggt gtaggtcatg agttttcata
    14521 caaacacttt tgcatagtca taaacaaaaa ggataataaa aataacggca aagtgaccgt
    14581 aatcccttta acttccaaaa gcaaacacaa tgcttttgta aatatacatc ttataaaacc
    14641 ttttttaaat acactaaaac aatcaattgc caaacacgag aaaaagatag aatcgattga
    14701 aaacattata aatcaaaatc ccaatgattt aaaagtcatt gtttatgcaa ggtttttgtg
    14761 tgactcctta aagaacgaaa tgaaacaata cgattttgtg gaaaacaaat taaaaaactt
    14821 agttgacaaa agttcttatg caaaaacaca ggatat
//
LOCUS       DAHHYJ010000059        14757 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000059 DAHHYJ010000000
VERSION     DAHHYJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.059"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(6..1064)
                     /gene="hisC"
                     /locus_tag="NBK22_001384"
     CDS             complement(6..1064)
                     /gene="hisC"
                     /locus_tag="NBK22_001384"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCD2414575.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEYFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKYE"
     gene            complement(1304..2818)
                     /locus_tag="NBK22_001385"
     CDS             complement(1304..2818)
                     /locus_tag="NBK22_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCD2414576.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQMMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            complement(2811..3788)
                     /locus_tag="NBK22_001386"
     CDS             complement(2811..3788)
                     /locus_tag="NBK22_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414577.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            complement(4010..5791)
                     /gene="recQ"
                     /locus_tag="NBK22_001387"
     CDS             complement(4010..5791)
                     /gene="recQ"
                     /locus_tag="NBK22_001387"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCD2414578.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQRIEKALSN
                     GEIQFLYVAPERFENRYFLNMLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFT
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQKIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRVLFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            complement(5803..7686)
                     /locus_tag="NBK22_001388"
     CDS             complement(5803..7686)
                     /locus_tag="NBK22_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD2414579.1"
                     /translation="MGMEAYKIEHLNKSYADKTIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKVAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQAEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            complement(7957..9897)
                     /gene="ltaS"
                     /locus_tag="NBK22_001389"
     CDS             complement(7957..9897)
                     /gene="ltaS"
                     /locus_tag="NBK22_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCD2414580.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            complement(10371..11375)
                     /locus_tag="NBK22_001390"
     CDS             complement(10371..11375)
                     /locus_tag="NBK22_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCD2414581.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMATIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVVKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(11359..12069)
                     /locus_tag="NBK22_001391"
     CDS             complement(11359..12069)
                     /locus_tag="NBK22_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCD2414582.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHNIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(12157..12363)
                     /locus_tag="NBK22_001392"
     CDS             complement(12157..12363)
                     /locus_tag="NBK22_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414583.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(12429..13037)
                     /locus_tag="NBK22_001393"
     CDS             complement(12429..13037)
                     /locus_tag="NBK22_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCD2414584.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESKFETIVSPLPEKSSFTAKFQVLPKVVNPTFIKNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(13037..14188)
                     /locus_tag="NBK22_001394"
     CDS             complement(13037..14188)
                     /locus_tag="NBK22_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HCD2414585.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLAKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKMEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(14172..>14757)
                     /locus_tag="NBK22_001395"
     CDS             complement(14172..>14757)
                     /locus_tag="NBK22_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCD2414586.1"
                     /translation="VIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMKPKA
                     IVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVHGHT
                     TQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDEAIIMAFEHITFPV
                     FGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
BASE COUNT         4588 a         2665 c         2019 g         5485 t
ORIGIN      
        1 acttactatt cgtatttaaa gtttgataaa acttctaaca ttttatcatt ttgttcttta
       61 aaaccaattg taattctaac accagttgga aatggtcgcg taatacaccc tacatttaaa
      121 agtgcttcat aaagttcatt tacccgcttt gtttttacaa atataaaatt cgtttgactt
      181 ggcaagaaat actcactttg aggtaattga taaaatcttt cgcgttcaac actattttta
      241 tgtgttacct cttttaaata ttgttgatct tcaagtgctg caactgctgc gtattcagat
      301 atacgtgtca cattaaatgg tggtctaatg atgttccatt tttcaatcgc atgttcactt
      361 gctaccacat atcctacacg taaacccgct aatccatacg cctttgaaaa tgtacgtaat
      421 aagaaagcat tgtcatattt ttgttgcaaa gcaagtgtat ccgggtagtc ctctgctgtc
      481 acaaattcaa aataagcttc atctataatt actggtacat gtggaggtac ttgagataaa
      541 aacgaatcta agctctcatg attaaaatat gtacctgtag gattatttgg attacataac
      601 cataccaatg acgtatcttc attaacttct tttaaaatac cttctaaatc gaagccacca
      661 tcttttaaag gtacttgtat cacattagct gattcaacaa tcgcattgtg ataatattga
      721 ccgaatgtcg cttcacttgt aacaatagta tcccctggcg ttaatacagc tctagaaatc
      781 attaatataa cttcatctaa tcccgcacca aataaaatgc gtgattgatc tacatttaaa
      841 tgtttactaa tcgccgcttt taatgtcggt gatcctgttt caggataata atataactca
      901 tctaagtgtg ctgatatcgc ttctttaact ttaggcgatg gtccatacaa attttcattt
      961 gatgcaagtt tatataaatc tccttcaatg ccatactttt ctttcaatgc ccttggagat
     1021 aaaccaggct gatatgctga tagttgatta agttgttctt tcataataaa aacaacctcc
     1081 ttagaattaa ctgaaatttt aaaaaactta tattcattat atcgctctat gtttctaaaa
     1141 tgcaatgcta ctattttcac atcataatag cttacattgt gtttaacatg tcatattttt
     1201 taatcattat tgcatgttta aattgaacat aattatagat ttaaatataa caacgccaca
     1261 taagatataa tttggtgtta cgcatcttat gtggcgttgg tcgttatttg attaaccctt
     1321 ttgcttttaa ataatctttc gcaactgtat atgggtcttc atttttaact gttactttat
     1381 agttcatcat ttgcatatct tcatcagata ttttgttttc tagtttgttt aacggtttct
     1441 taatttctgg atgtttcttt aaaaagcttt ctttaaataa tggtgctcct tgatatggtg
     1501 gaaatacgtg cttatcatct tttaacacaa ccatatcata ttgttttaat tcagcgtcag
     1561 ttgaatatgc atctattaaa ttaatattac ctttattgat cgcttgatat ctcaacttag
     1621 gttccattgt acgtatgtta tctaaattta aattataagc cttttgaaca gctttgtaac
     1681 catctggacg atcattaaat tccaatgtaa atcctggttt aagttgatct ttaaccttat
     1741 ttaaatcacc aattgtacgt atattatgtt gtttagcaaa atcacgtttt acagctaaag
     1801 catacgtatt gttatacttc attggtttta acatagtcat ttgatatttc ttttcaagac
     1861 tttgcttagc ttgttcataa acttttttct cttcttttga cttcaatggt tcttttgtta
     1921 attcacctaa aactgttcca gtaaattcta aatacccatc tatatcgtca gattttaaag
     1981 cattaaataa aaatgctgtt ttgcccatac catctttcac ttctacagta tttttggtct
     2041 cttcttctat taaaatttta tacatatttg taataatcga tggctcggag ccaagctttc
     2101 cagctaacgt aattttatca cctttttgtg caaacatagg aatagcgata gccagtataa
     2161 taatcatcac tataacacct aacgtcatca ataacttttt ataagataat ttagccataa
     2221 atcttaaaat taaatcaaat ataattgcca ataaggctgc tggaattgca cctaataata
     2281 tcaacgatgc attgttacgg tctataccta ataaaattaa atctcctagt ccgcctgcac
     2341 caattaatgc tgctagtgtt gctgtaccta taattaatac catagccgtt cttacaccag
     2401 ccattataac aggcattgct atcggaagtt cgactttagt taaacgtcta aatggtttca
     2461 tacctatacc tttagccgct tcaatgagtg atggatcaac ttctttaatt ccagtatacg
     2521 tattccttaa aattggtaac aacgcataca ctacaagtgc aataattgct ggcacacgac
     2581 cgataccaaa taaaggaatc attaaaccta ataatgccaa cgatggtatg gtttgaagaa
     2641 ttgccgcaat attcattacg atttcagata tcgttttagt cttcgttaat aaaataccta
     2701 atggtaccgc aatagcagtt gcaatcaata atgcgataaa tgatatttga atatgttcta
     2761 tcattgtcga aaagagttgc cccttacgtt cactcaatat gtcgaaaaag ttagtcatgt
     2821 tgagctacct cctttttctg ggacaaatat ttgaagatat ctttcctatc aataacatat
     2881 tgacctacgc tatcttcttg catgacaatg acacgctcgc tctctgataa aagttgatac
     2941 aatacttcaa ttggttgatt gtcataaaca attggataag cgctcataga tgtaacctca
     3001 tcgattggtt tcataatatc caagtcacgg ataattgcgt tctcttcaac acatggcgca
     3061 tcatcttcta aatgactacc cataaattgt ttaacaaatt cactttgagg attattttta
     3121 aatccttctg gtgtgtcaat ttgttcaata tgcccttcat tcaaaagaca aatcttatca
     3181 ccaagtttca tcgcctcttg aatatcatgt gtaacaaata tgattgtctt cttaatttta
     3241 gtttgtaatt caattaaatc atcttgaagt ttttctcggc tgattgggtc taatgcacta
     3301 aacggttcat ccattaaaat aactggtgga tcagctgcta acgcacgtat aactcctaca
     3361 cgttgtcgtt gcccccctga caattcatca ggttttctgt ttttatattt ttcaggttct
     3421 aatccaacca tttcaagtaa ttcatctact cttttatcta tatctttttc tttccacttt
     3481 ttcatttgtg gcacttgtgc aatattttct ttgattgtca tatgtgggaa taatgcaatc
     3541 tgctgcaata cgtatccaat atcccaacgc atttcgtata ctggataatc acttattggt
     3601 ttatctttaa aataaatata accttcactt aagtgaatga gtcgattaat catttttaat
     3661 gtcgtagttt ttccacaacc tgaaggtcca attagcacaa aaaattcacc ctcattaata
     3721 ttgaaactaa tgttatcgac agcaacatgt ttgccataac gcttagttac atttttaaac
     3781 ttaatcactt tgccacctct ttttttctca tagcataaaa ccgagattat atgtatgtat
     3841 tccctattta accacgttta ttacaatttt caaatttaaa tgatttatcc ttgaactttt
     3901 ttaataaaat aatgaataat aggtaatctc cagttaagaa atagtgttat tttaccttga
     3961 attcaaaaaa acacccagta aaacaaggaa tgcttactag gtgtcttcac tatactttgg
     4021 ctttataatt ttgaatcgtt tctaaaaatg ctggacaata atgttttaat ttgtaactac
     4081 ctacgccatc aatattaatc atatcttgtt tcgaagcagg cttacgttta gcaaattcct
     4141 ccaacgtgta atcagaaaat atacttacag gtgctatcgt taatttgtca cttaactttt
     4201 tacgaacttc taccaactga ctgaataata ctcggtcaac cccttcaacc gtatttataa
     4261 atactttttc agtcgctttt tgcttaaatg gtgttgtgaa tacttctact tcattactga
     4321 gtaatttttt aattgaagta tcacacatta atatttcatc attttcattt aagaaccctt
     4381 tgaatcttaa ttcatctatt aagtgactta attctgatgt tgtgtaacct ttcattaaac
     4441 catgggttga aatttgatca taacctttgt acttaatata atctgttgac tctcctctta
     4501 gcacttgaat gataacacta taactctctt gttgtttcat acgagcgatg caactaataa
     4561 tcatcttagc ttcttgtgtc atattatatg atttatcttg ttgaacacaa ttactacatt
     4621 gttcacattc ttctaatttt tcattcggtt caaaataatg gacaattgtt gcttctaaac
     4681 atttttttgt ttttgtatat tgaatcattt tagttaactt ttcgcccatt ttatctttat
     4741 agtcatcatc agcttgagag actgttataa aatactcgtg taaattgata tcgcgttcgc
     4801 taaataacaa aatacattca cttttcaacc cgtcacgacc tgcacgaccg gcttcttgat
     4861 aataagattc taaatctcca ggcatattat aatgaataac aaaacgtaca ttggatttat
     4921 caatacccat accaaaagca tttgtagcaa cgactacttt aacacgatca aataagaaat
     4981 cattctgcgc ctcttctctt tctttattgc tcaaacctgc atgatatata acactttcaa
     5041 ttttctggct ttctaaggct tcttgaagct cttcaacttg cttacgtgta gaacaataaa
     5101 taatacctgc atcttcatca tgtgttttaa tataatccaa tataaatttt tgacgttgat
     5161 aagtaggatt tactttaaaa attaagtttc tacgcttagt actcgtttta atttgatcag
     5221 tttgagcgat atttaacttt tctctaatat cttgctgtac ttcaaccgtg gcagttgctg
     5281 tcaacgctat tattgtaaaa tcttgaggta acgtaaatac ttttgaaata acattttggt
     5341 aactcggcct gaaatcatga ccccatttag aaatacaatg cgcttcatca aacgcgacta
     5401 agtgaatctt tatacgctga agcatattta aaaaatatcg gttttcaaat cgttctggtg
     5461 caacatacaa aaattgaatt tctccatttg ataatgcttt ttcaatacgt tgttgctctt
     5521 tttgagtcaa actactattt aaaaaagcag cttgaattcc catcgctttt aattgatcca
     5581 cttgatcttt cattaatgat attagtggac ttattacaat tgttgtacca cctaacaata
     5641 aacctggtac ttgatagcat atagacttac ctccaccagt tggtaagaca ccaagcacat
     5701 tacgatggtc taatactttg ctaataattt cttcttgtcc tggtcgaaac gtttcatacc
     5761 caaagtaatg cgataatgtt tgttgcatca taaattgacc ccttattgtt gttctttaat
     5821 ttcttctaac tcactccatc ttgtgatgtc taaatcatat tgaatttcaa gttgttcttt
     5881 ttcttcgttt aattctttaa ttttcccata atctgcactt gcctcaatca tgagcacatc
     5941 tatttcttcc attcttactt ccgcttgttc tatgcgtttc atcaattgtt catattctaa
     6001 cttttcttta tatgataaac catttttctt acgtacagtt gtagaagatt tagattgttg
     6061 cttcaatgtg gatttatttt tatctaatga ttttttataa ctttcataat cttcaaaagt
     6121 tccgataatc ttttccatct gaccatcatg aataaaccaa tatgactgtg caactttatt
     6181 taaaaagtag cgatcatggc ttacggtaat cactgtacca ccgaaagtat gaatataatc
     6241 ttcaagtatt gttaaagtct cagtatctaa atcatttgtc ggttcatcta acaacagaac
     6301 atttggctgg tgtacgagta gacgtaataa atacaaacgc ttttgctctc caccagataa
     6361 tttataaact tttttaccat gagttgcact tggaaataaa aatcgttcaa gaagttgtgt
     6421 aatagatacc gaggttccat ctttttcttt tgcgatctca ctttcttctc ttaaataatc
     6481 aatcatacga atatctctat ccagggtctc atctgtttgc ttaaaataag ctactttaac
     6541 cgtctgtcca gtcttcaatt taccttcgaa ttgttggtct tctccactca aaatatttaa
     6601 gagtgttgtt ttaccagctc catttggccc aacaacacca atacgctcac ctttttgaat
     6661 aatttccgtc agatgttcaa ataatacttt atcattaata gcctttgata agtcttctaa
     6721 ttcgaacact tgcttaccta atcttgaata agcaagattc aattcacctt tatcgtcttt
     6781 atattgctgg ttaacttcat tttctaggtc attaaatcga ttaattctag cttgttgctt
     6841 tgtagtacga gccttagctc cagccctcat ccaagcaagt tcttccttat ataaagctcg
     6901 ttgcttttgt tgttgctttt gaagtgttac ttctctttca gcgcgcattt caatataaga
     6961 ttcatagtta ccaggatatg acgctaactt acctctgttt agttcaataa ttctagtgga
     7021 aacttcattt aaaaaatatc gatcatgggt tacgaataaa acagtatgag gatattgctt
     7081 cacataattg atcaaccagc tgattgattc gaagtctaaa tggttcgtag gttcatctaa
     7141 taacaataaa tctggttgtt ctattaatgt tttagcaagt acaacacgtt tttgttgtcc
     7201 gccggataat tctttaatgt atttagtagt atcatgtata cctagttttg agaggattgt
     7261 tttaatttca gcgttatagt cccaagcatc atgttgatcc atagcatctt gcgcatccat
     7321 cattcgcttg aacaatttat cactttggtc atccgcataa gcttgtactg cctgctcata
     7381 ttgcttgatg atgcgtaaag ttgttgtatc agaacttaat actgcatcaa aaactgtcat
     7441 atcttcatta aggtcctgtt tctgagacga atatcgaatt cgatattgat ttggatgcat
     7501 aacattggct gtaaaatcat catcaatacc accaattact tttaacaacg tacttttccc
     7561 tgtaccattt atgcctacta aacctatttt ttcaccttct gaaattgata aatctaggtt
     7621 atcgaatata gtcttatcgg cataagattt atttaaatgt tcaattttat atgcttccat
     7681 acccatcttc cttcttctgt tgaaatctct acgtacttaa atcagttcta tacattatac
     7741 acctttttga aatcaaaaga aatcgatacc gttaagtgtc ataatttaat atattgttgc
     7801 ttcaactata aataaaactt gttaaggctt tgatgactat gctaatgatc tttataatat
     7861 acctttttat caacacatat aatgtaaaac ataatataaa agaacataat aaaaacccgg
     7921 attgatattt tatcaatccg agttcgtgtt taaatattat tttttagagt ttgctttagg
     7981 tcctgtttca tatttatact tcgaaggatt tacctttttg aagtctggat ttttgtagaa
     8041 tctaaacaaa tcaccattaa gcacgttgtc actcatttcg agatcctttt caacttgctt
     8101 tttattcttt tcgaaatcag ctggttgagt agttatgagt tcattatttt tattagaata
     8161 aatcttacca ttaacatatt tataatcttt tgttataaag tcaccatttc tgaatggaac
     8221 tacttgatta tgacctttag agaataaatc agtaccgaac attaaataat tcttcgtatc
     8281 tataccagcc aaatgtaaaa ttgttggcat tacatcgact tgaccagcat attcattatt
     8341 gataccacca gatttaccag ggattttaat ccagaaacca gttctgttta aatctgtaaa
     8401 tttagctggt gtgatttttt cacctaatag tttttccatg gcattgttat ggttttcaga
     8461 gataccatag tggtcaccat aaatcataat cactgaattg tcatataatc cttttttctt
     8521 caagtcatta atatattctt ctaatgcttc gtctaaataa cgcgctgttt gaatataacc
     8581 atcaactgtt gcatcacctg tgtttgactt ctcaatagtc gcatcctttt catctaatgt
     8641 gaatggatag tggttagtca aagtaattaa atgagaatag aatggtgatt tcatcttagc
     8701 ttgataatta gcagaatctt taaagaaaat tttgtctttc aagcctaagt ttacaacgtt
     8761 tttatcagac atgtcatagt atgttgcatc atagaattta tcgataccaa agtgtttata
     8821 tacttggtct ctgttccaga atgttttata gtcaccgtgc atgacatcag atttgtagcc
     8881 ttgcttttga tctaaaattg ctggtagtga ctgatacgta ttatctcctt ttaatgaaaa
     8941 ggcagaacct tgcggtaaac cgtataaact gttatccatt gtaaattcag agtcagatgt
     9001 tttaccttga cctgtttgat ggaaaaagtt agggaagtat gtgaattgct ctttcccact
     9061 tgataatttg tttaaaaacg gtgttacttc tttaccatta acctttttat taattaagaa
     9121 ggtttggaaa ctttctaaat gaatcttaat aatatttttc ttctttgcca ccccataata
     9181 ttctgggtta ggctctgttt gacgttgttt cgtataattt aatacttttg ttaagtcatc
     9241 ttcagatgct aacgcttttt gttgattatt ttcgatagtt ttaacaccat cgtatactgt
     9301 aaagttatac ggtcctaaat atttcactaa atatttatgg tcaaatgtac gtgttaataa
     9361 ttctggtctg tcagtttcag caaaagctaa gtttaagaag aataatgcta ctgaagccgc
     9421 cattacgaca ggaacaaatt tcttactaaa tgcttttgtg tctaaccatt ttgttttaaa
     9481 tattaaaatg aataagtaaa ctaacgtatc aatgaaataa acaaagtcat accatttgaa
     9541 tgatgcacta accgcaccac ccatagattc tacgttacct acttggttta aagtactaaa
     9601 cgttaaaaaa tccgagaaga atctaaagta cacaacattg gcatataata ggaacgtcaa
     9661 taagaagccg cctatgaaca tgaaccaaaa tgcttttttg cctttaaaga ataggaacac
     9721 acttaaaacc agtgctacta aactataagg attcatcaat aatattaagt tttgtactaa
     9781 acctttaaca cctaaagaaa aatcaacata ataagaaaaa tacgtcttca aggtaatcgt
     9841 tattacggtt aataagaaga acgcaaaaag actaattttc tttttttgtg aactcatgat
     9901 tctttccccc gttatttaga taataaatct tgcgaaagta cttgaataaa ttagatatta
     9961 acattatgta gttaattaat aagtgcgttt aaaagttaac attgtcataa ttatagtaat
    10021 tactttcaga ttcaagttta taattttcat tatttgtagc tttcaacttt gatttaacaa
    10081 gtattttggg ctacaactca tatttgcttg tgtaagtttc tttatttaat attatcattc
    10141 aattaacaaa tttaaaattt gtaatcaaat tgtaataata agcatagctc aattaataaa
    10201 tatagagtaa ttttagtcaa aattcaagta aaaacacact tcatcttttc attttactgt
    10261 gaaacgaatt agaatatgat acatttcgca gataaaacac agtattattt cattatacat
    10321 taggaggaaa ttatttgcat cggactcgag tatgagctac gtaccaaata ttatttcatc
    10381 atttctaatt gcattctata ttctattcca tggaataatt ggtttaacca gttactatac
    10441 ttaacaaggt tcttttcagc cgttaaaata tctataaatt gaaatttcaa acgtgatcct
    10501 tgcggttttt gtgctaattt cgttaaatga taacttgcga ttgtaccgat ttgcgggtag
    10561 ctacctagcg tataatgatc atttaataag ataataggtg tgccatcacg cttaatttgt
    10621 atcgttcctt ttttgacagt ctgatacggt ggcatatctt cataaaaggc tttgattttt
    10681 tcaccttcaa gcatcatccc catgcgattt gctttgctcg ttactttata atcatgtttc
    10741 acaaatcttt gaatggcatc ttctttaaaa tcttccgtac ctttattttt aacgacatga
    10801 aatacatcag acatataatt aaatgacaag gcgtatccat caatacccca atctgtttgt
    10861 ttcgtgtgag caaggttttc aaataactta tgatgacgag ctgtataatc tctcttaagc
    10921 tttatttcat cgccatcttg taatgttcta cctttaaaac caccaatttt tacattaaag
    10981 tcggttgagt tagatcctaa ccatgcatct aattcaaatc cgcctcccac agctaaatac
    11041 actcgcgatg tataacttgt ttctctaaac tttaaaacat cgcctttatc taacaaatac
    11101 aatttatatg gagatatagt catatgctca gtgtaagctt tgacattacc ccctgctaat
    11161 gcaatcagcg taggttctgt aaaacgaatc gttgccattt tattagtcat ttccaaagtt
    11221 gcttcattct tgtcatttgc aactaatcga ttagcaattt catgtgctaa agtatcaagt
    11281 gcaccacatg gaattacacc atcatgttca tatccccttc tgccaaagtc ctgaaagcta
    11341 ctgaataagc cacttttttc aattatgatt gacatcttta aacgctccta cttctaattc
    11401 attttcattg ataggtctaa atttaatatt atcgcctaac tttaagcgtg cgaaatctga
    11461 ttctttcgga ttaaaaagtg ataatggtgt ataaccaata actaaccaat cattaattgt
    11521 atccgtagtt acaataccgc attttttccc ttcaagtact acagaaccag ctggaatgaa
    11581 ttttttctgt ttactcgtgt gattaatata caatttctta tttaatccag ttaaataagg
    11641 aaacccaggt gaatatccca tcatcgaaac aaaatattgc gccttagaat gtaattcaat
    11701 aaaagtttct agcttgattt tgtaatgttt taaaagtgat tctaaatccg gaccatattt
    11761 cgaaccatac acaattggta tttcaatcaa atcttgctgg ttaaaatcat gttttatgtt
    11821 taaatgaatc gattctatta gtgctttcat gtataaaaaa ggtgattgta tattatggtg
    11881 tttaatcata tcccttgcgt catagacaat catcatgtct gattctgatg gcacaatttc
    11941 tataataaat ggataatttt gttcaattaa atatgagcga agtgttaata aatcttcggt
    12001 taaactttta gatacttctt tttcaattgc gactacaatg gcttggtcac cttgactata
    12061 tattttcatt tgtacacctc ttcaatattt ggttacttta ctttgatttt taattataga
    12121 atcgattgaa tagttcattt tacctactca ctaaatttac tggtgataca ttaaatccat
    12181 tgcccgcaca acaaaaggca tgagttcgtg aattaatgcg tatacaagga taaagcttat
    12241 aacagtagta attgttgcta tcaaacgaac aacatatatt ctattttcag atagcaatcg
    12301 attcactaat cgataattta cgtatacaag aattaacaac agcacaatat aaacaataat
    12361 catatttggc cccatttaat ttttaaattt gttttcacat cattttctac attattatca
    12421 tactaatttt aaaggcaaag gtggacatcg gcaacctctc gtaaactatt tatcaaaaat
    12481 aaacgtatct cattgttatg atatttataa atcaattcgt ttttattaaa gtctttttca
    12541 acaagcttgt cactatctat taaataatca cgcatgcatc cttttaagaa atcatcttta
    12601 taacttggtg tgtaccattt tccatcctct tcaatgacaa tgttgccaat atcaaattca
    12661 aggaccttgc cgtcctctga agttagcaat attaaatctg tttcatgatt gtgtgctaaa
    12721 tgctttcgtt ctgtcgtttt attttttata aaagttggat taactacttt gggcaacact
    12781 tgaaattttg ctgtaaaact acttttctca ggtaaaggtg acactatcgt ttcaaatttg
    12841 ctttcagcat ttaatacgat tttaagtcta tattgaccac tgtgatactt tgttgttaca
    12901 tcgtttaatt catttcgcca tgcatgttca tcaaatttaa agtttaatcg ctcagaagaa
    12961 cattttatgc gattagtatg ataagtaagt ctagggatat gtccattatc aattttcatt
    13021 gtttcaaata attgcattat aacatctcca aaatcttggt ttttgcgtaa aattcattca
    13081 cttcatcttt tggcttagaa tcaattgtaa tacctgctcc gactccataa atcgcttgtc
    13141 catatttata ctcaatagta cgaatcggaa tattaaaaat cattttatca tcttcagttg
    13201 gaagtaatag tccaattgct ccgcagtata taccacgagg tgaactttct aattgtttaa
    13261 tatatttcat tgtattcagt ttcggtgcac ctgtaatcga accacaagga aataacgatg
    13321 ttaaaatttg agttaagtct gtattatttt ttaaagttcc acttaccatc gaagtcattt
    13381 gaaatacagt tttatatgcc tcaataaaaa atagtttata caccttaatt gagccactct
    13441 gtgatattct ccctatatcg tttcttagta aatcaacaat catgacattt tctgcacgat
    13501 ctttcgaaga agtttgcaat gttttataat actgttgatc ttcagcttcc attttacctc
    13561 taggcattgt ccctttcatc ggtttgctta tgataacgtt atcgacattg ttaaactgtc
    13621 ctttttgaaa aaataattct ggtgagatag acgctacttg gatttcatct gtttgtaata
    13681 acgcagtata attaccatta ctaaattgag ttaatcgttc atataaagta ctaataggat
    13741 aataaatgtt atccgttaag cgcgccgtat agttcacttg atacgtttcg ccttcaacaa
    13801 ttgcttgttg gacacgttta atattagtca tcataacctc agaagattca acaaatgaaa
    13861 aatgatgctt tgatacataa gaagtttgat gttcatatgt tgaatttatg ctttccgctt
    13921 tttcaaaact ataagctact gcataaatat catctttagc taatgaatgt gtacacataa
    13981 catgattaaa atactttgcc gcttcgtaac ttaaatataa cgagacatat ctaccttgtc
    14041 gttgctgcgc ttgtgcaaag tgtatcactt ctcccacatc agccaactta gtagcaacat
    14101 acttctttat aaatcccttt aattgaatat ggtattgctt atattcattt tcagttaaat
    14161 agtagcgata attatattct attctcacag taatcaccta ccttcgataa aaataattca
    14221 acttgtcgat aaccgtattc actcaaaata gattcaggat gatattgcac accaaaaacc
    14281 ggaaatgtaa tatgctcaaa tgccataata atcgcttcat cgtttttggc tgtaatcttt
    14341 aagcaatttg gaaaagtcgc tccgtcagca attaatgaat gataacgcat tacattgaaa
    14401 ttttgaggca gtccttgaaa aataccttca ttggtatggc gtaactgtgt agtatgtccg
    14461 tgtacaggat gatagccgtg aatgatattt ccaccaaaat aagacacgat acattgaaat
    14521 cctaagcata cacctagtat aggtacacgc tgataaaatt gttctaacac ttcattcaat
    14581 ataggataat catccggatt acccggccca ggtgaaataa caattgcttt tggcttcata
    14641 ttaatgacgt cttctatcag cagattatca ataccaacaa cttgaactgt tagtttcgtt
    14701 tgagtcttaa tatagtctat taaattatat gtaaatgaat cattattatc tatgact
//
LOCUS       DAHHYJ010000060        14730 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000060 DAHHYJ010000000
VERSION     DAHHYJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14730
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.060"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            97..888
                     /locus_tag="NBK22_001396"
     CDS             97..888
                     /locus_tag="NBK22_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD2414587.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            1256..1684
                     /gene="lacA"
                     /locus_tag="NBK22_001397"
     CDS             1256..1684
                     /gene="lacA"
                     /locus_tag="NBK22_001397"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCD2414588.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            1700..2215
                     /gene="lacB"
                     /locus_tag="NBK22_001398"
     CDS             1700..2215
                     /gene="lacB"
                     /locus_tag="NBK22_001398"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCD2414589.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            2228..3160
                     /gene="lacC"
                     /locus_tag="NBK22_001399"
     CDS             2228..3160
                     /gene="lacC"
                     /locus_tag="NBK22_001399"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCD2414590.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHANIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLKNPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGAQGAFAKHNHTFYRVNIPTISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDELFNQIEVLEV"
     gene            3164..4144
                     /gene="lacD"
                     /locus_tag="NBK22_001400"
     CDS             3164..4144
                     /gene="lacD"
                     /locus_tag="NBK22_001400"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCD2414591.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDAEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATQLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            4166..4477
                     /locus_tag="NBK22_001401"
     CDS             4166..4477
                     /locus_tag="NBK22_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD2414592.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            4483..6195
                     /locus_tag="NBK22_001402"
     CDS             4483..6195
                     /locus_tag="NBK22_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD2414593.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMIAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            6213..7625
                     /gene="lacG"
                     /locus_tag="NBK22_001403"
     CDS             6213..7625
                     /gene="lacG"
                     /locus_tag="NBK22_001403"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCD2414594.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEYCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            7891..8760
                     /locus_tag="NBK22_001404"
     CDS             7891..8760
                     /locus_tag="NBK22_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2414595.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGELRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            9042..10043
                     /locus_tag="NBK22_001405"
     CDS             9042..10043
                     /locus_tag="NBK22_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCD2414596.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEDIDTPKLESDGVLVQMLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            10277..11284
                     /locus_tag="NBK22_001406"
     CDS             10277..11284
                     /locus_tag="NBK22_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD2414597.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHIATIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHQLLEKQSMIGKLVIN
                     I"
     gene            11592..13154
                     /locus_tag="NBK22_001407"
     CDS             11592..13154
                     /locus_tag="NBK22_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD2414598.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLCNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            13329..13865
                     /gene="amaP"
                     /locus_tag="NBK22_001408"
     CDS             13329..13865
                     /gene="amaP"
                     /locus_tag="NBK22_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCD2414599.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            13878..14117
                     /locus_tag="NBK22_001409"
     CDS             13878..14117
                     /locus_tag="NBK22_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCD2414600.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTVLIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            14180..14689
                     /locus_tag="NBK22_001410"
     CDS             14180..14689
                     /locus_tag="NBK22_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD2414601.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
BASE COUNT         5186 a         2122 c         2876 g         4546 t
ORIGIN      
        1 acatataatc gaacagttaa attatattag tttttacgca atttttatta tagaataagc
       61 atgtatatta aaattgatgt ctaaacataa aggataatga cccatcgcct aaataaagga
      121 ggcaatttta cattgaataa acatgaacgt ttggacgaaa ttgctaaact agtgaataaa
      181 aagggcacga taagaacgaa tgaaatcgtc gaaggtttaa atgtgtctga tatgacagtt
      241 cgaagagatt taattgaatt ggaaaataaa gggattttaa cgaagattca tggtggtgca
      301 cgcagtaatt caacatttca gtataaagaa atatcgcata aagaaaaaca tacccgtcaa
      361 attgcagaaa aacgatttat agctaaaaaa gctgcatcat taattgaaga tggggatact
      421 ttgtttttcg gaccaggaac aacagtggaa ctattagcag aagaagtcaa tcatcatacg
      481 ctcacaatta ttacgaattg tttgccggtg tataaaattt tgttagaaaa acaaacagca
      541 cattttcgtg tctatttaat tggtggtgaa atgcgccata ttacagaagc atttgtaggt
      601 gaaatggcga acgccatgtt ggaaaaacta agatttagca agatgttctt tagtagtaac
      661 gcagtaaata aaggtgctgt gatgacatct acattagatg aagcctatac gcaacaactg
      721 gcactcagta attcaattga aaaatacttg ttaatcgatc atacgaaagt tggcaaagaa
      781 gattttacat cattttgtca gttaaacgaa ttgactgcag tggtcatgga ctatgaagat
      841 gaagaaaaag tagaaacgat taaaacatat attgaagtag tcgactaaat taaaagtata
      901 actgcattgt taaaatatgt atctatatgc tcataagtga atgtgaattt gcattcattt
      961 atgggctatt ttttatgcaa atttatgtca acgggaacct cgacaaagtt ttactacaat
     1021 ttcagacgaa tacctaatta aaacaacacc acaatatgaa tgttcactta ttgtttgaaa
     1081 atacgtcata aacaaacaga ttataaacat attatgttga tttattgttt gtttatgttt
     1141 ataatttaaa cgtaatcaaa ttgaaagcct tttctcaaga ttttcataac ctacaaataa
     1201 atatgtcaca ttttaagaaa ggtatttcaa aattaaagta aaaaggagtc ttattatggc
     1261 gattattatt ggttcagatg aagctggcaa acgattaaaa gaagtcatca aatcatactt
     1321 attagacaat aaatatgatg ttgttgacgt aacagaagga caggaagttg actttgttga
     1381 tgcaactttg gctgtagcaa aagatgttca aagtcaagaa ggtaacttag gtattgttat
     1441 cgatgcattt ggtgcaggta gctttatggt tgctacaaaa atcaaaggca tgattgctgc
     1501 tgaagtatca gatgaacgct ctggatatat gactagaggc cataacaatt caagaatgat
     1561 cactatggga tctgaaattg ttggagacac attagcgaaa aatgtcgtta aaggttttgt
     1621 tgaaggtaaa tacgatggtg gtagacacca aatccgcgta gatatgctta ataaaatgtg
     1681 ctaaggagga aaaattacta tgaagattgc attaggatgc gaccatattg ttacagatac
     1741 aaaaatgcgt gtatctgaat ttttaaaatc aaaaggacat gaagtcattg acgtaggaac
     1801 atacgatttc acaagaacac attatccaat ttttggtaaa aaagttggcg aacaagttgt
     1861 tagcggtaat gcagacttag gtgtttgtat ttgtggaaca ggtgttggta ttaacaatgc
     1921 tgtaaataaa gtacctggcg ttcgttcagc actagtacgt gatatgacat cagcgttata
     1981 tgctaaagaa gaattaaatg cgaacgttat tggctttggt ggacgtatta taggtgagtt
     2041 attaatgtgc gatattatcg atgcatttat taacgctgaa tataaaccaa ctgaagagaa
     2101 caaaaaatta atcgctaaaa ttaaacattt agaaacaagc aatgcagatc aagctgatcc
     2161 acatttcttt gatgaattct tagaaaaatg ggacagaggc gaataccacg attaagggac
     2221 gacacatatg attttaactt taacattaaa tccatccgtt gatatttcat atccattaac
     2281 agcattgaaa ttagatgatg ttaatcgtgt tcaagaagta agtaaaacag ctggtggtaa
     2341 aggtttaaat gtaacaagag tacttgcgca agtaggcgag ccagtccttg cgagtggttt
     2401 tattggcggt gaattaggtc aatttattgc taaaaaatta gatcatgcca acatcaagca
     2461 tgcattttat aacattaagg gtgaaacacg aaactgcatt gcaattttac atgaagggca
     2521 acaaacagaa attttagagc agggaccgga gattgataat caagaggctg caggatttat
     2581 taaacatttt gaacaattat tagaaaaagt tgaagcagtt gctatttcag gtagcttacc
     2641 taaaggatta aatcaagatt attatgcaca aattattgaa cgatgccaaa acaaaggtgt
     2701 gcctgtgata ttggactgct cgggtgcaac tttgcaaact gttttgaaaa atccatacaa
     2761 accaacagtc attaaaccaa acatttctga actatatcaa ttgcttaacc agccacttga
     2821 tgaaagttta gaaagtttga aacaggcagt gtcacaaccc ttattcgaag ggattgagtg
     2881 gattattgtg tctttaggtg cacaaggtgc attcgctaag cacaatcata cattctatag
     2941 agtgaatatt ccgacaataa gcgtattaaa tccggttggt tcaggtgatt ctacagttgc
     3001 tggaattact tcagcaattc tcaatcatga aaacgaccac gatttactaa aaaaagctaa
     3061 tactttaggc atgctgaatg cgcaagaagc acaaacagga tatgtcaatt taaataatta
     3121 tgatgaatta ttcaatcaaa ttgaagtttt agaggtgtaa ataatgtcaa aatctaatca
     3181 aaaaatcgca tcaattgaac aattaagtaa taacgaaggt atcatttctg cattagcatt
     3241 tgaccaacgt ggtgctttaa aacgaatgat ggctaaacat caaacggaag agccaactgt
     3301 cgctcaaatt gaacaattaa aagtattggt tgcggaagaa ttaacacaat atgcgtcttc
     3361 aattttatta gatccagaat acggtttacc agcatctgat gcacgtaaca aagattgtgg
     3421 attattgcta gcttatgaaa aaactggtta tgacgtaaat gctaaaggtc gtttacctga
     3481 ctgtttagta gagtggtctg caaaacgttt aaaagaacaa ggcgcaaatg ctgttaaatt
     3541 cttactttac tatgatgttg atgatgctga agaaattaac attcaaaaga aagcatacat
     3601 tgaaagaatc ggttcagaat gtgttgcaga agatattcca ttctttttag aagtattaac
     3661 atacgatgac aacattcctg ataacggtag tgttgaattc gcaaaagtta agcctagaaa
     3721 ggttaacgaa gcaatgaaat tattctcaga accacgcttt aacgttgatg tattgaaagt
     3781 tgaagtacca gtaaacatga aatatgtaga aggctttgct gaaggtgaag tggtttatac
     3841 aaaagaggaa gcggcacaac actttaaaga tcaagatgca gcaactcagt taccatacat
     3901 ttacttaagt gctggtgttt ctgcggaatt attccaagaa actttaaaat ttgctcacga
     3961 agctggtgct aaattcaacg gggtactttg tggccgtgct acatggtctg gtgctgttca
     4021 agtatacatt gaacaaggtg aagatgctgc tcgtgaatgg ttacgtacaa ctggtttcaa
     4081 aaatattgat gacttaaata aagtattaaa agatactgcg acatcttgga aacaaagaaa
     4141 ataagtttaa aaggggatta atgctatgaa tagagaagaa gtccaattat taggttttga
     4201 aattgttgca tttgcagggg atgcacgttc taagttttta gaagcattaa cagcagctca
     4261 agctggagat tttgcaaaag cagatgcact gattgaagaa ggaaacaatt gcattgcgga
     4321 agcgcataga gcacaaacaa gcctgttggc taaagaagcg caaggtgatg atattgcata
     4381 cagtgtaacg atgatgcatg gtcaagatca tttaatgaca acaattttac tgaaagattt
     4441 aatgaagcat ttattagaat tttataaaag agggtgatga caatgatgca aaaattgatc
     4501 gcacaaattg aaaaagggaa gccatttttc gagaaattat cacgtaacat atatttaaga
     4561 gccatccgtg atggatttat ttctgcaatg cctgtcatct tattctcaag tatctttcta
     4621 ttaattgcat acgtgccaaa tatatttggt tttaaatggg ataaaggcat ggaagccatt
     4681 ttaatgaaac catataacta tacgatgggg cttgtagcat ttttagtagc aggaacgaca
     4741 gcgaaatctt taacagattc atttaaccgt aagctagaaa gtacaaatca aattaacttt
     4801 atttctacaa tgatagcagc gatgtgtgga ttcttgttct tagcttctga tccagccaaa
     4861 gatggtggat tcttaagtgc atttatggga actaaaggtt tattaacagc attcttatca
     4921 gcatttgtaa ctgttattgt ttataacttc tgtgtgaagc gcaatattac aattaaaatg
     4981 ccgaaagaag taccgccgaa tatttcacaa gtatttaagg acttaattcc attttcagcg
     5041 gtaatcatca ttctttatgc attagattta gtcattcgca acagctttaa atcaaatgta
     5101 gcggaaggta ttttaaaatt attcgaacca ttatttacag cagcagatgg atggattggt
     5161 gtcacaatta tctttggtgc ctttgcatta ttctggtttg taggaattca tggtccatca
     5221 atagtagagc cagcaattgc agccattaca tatgcgaata tcgaagcgaa cttcaagttg
     5281 cttcaagctg gagaacacgc agataaaatt attacatcag gtacacaaat gtttatcgtt
     5341 acttttggag gtacgggtgc aacattggtc gtcccgttca tgtttatgtg gatgacgaaa
     5401 tctaaacgaa acaaagcgat tggacgtgca tcagttgtac caacattctt tggtgtaaac
     5461 gaaccaattt tatttggtgc accactagta ttgaatcctg tgttctttat tccatttgta
     5521 ttagcaccaa ttgttaacgt atggattttc aaacttttcg ttgaagtgtt aggaatgaat
     5581 agttttagtg tgaatttacc ttggacaaca ccaggtccat tgggcattat catgggtaca
     5641 ggttttggtt tatggtcatt cgtactagct attactttga ttgttgtaga tattattatt
     5701 tactacccat tcctaaaagt ttatgatagt gaaattcttg atgaagaaga aggacgtaaa
     5761 gaaagtaatt cagatttaaa agaaaaagtt gcagcaaact ttgatacgaa aaaagctgat
     5821 tcaattttag cggcaagtgg tgtatcagac gatgcagcta aagcttcaaa tatcacagaa
     5881 caaacaaatg tcttagtact ttgtgccggt ggtggtacaa gtggattatt agccaatgca
     5941 cttaataaag cagctgaaga atatcatgtg ccagtgaaag cggcagctgg cggatatggc
     6001 gcacatatgg acattatgaa agagtaccaa ttaatcattt tagcgccaca agttgcgtct
     6061 aactatgaag atattaagca agatacagat agattaggta ttaaattagc gaagactcaa
     6121 ggtgccgaat atatcaaatt gacacgagat ggccaagcag cccttgattt cgttcaacaa
     6181 caatttgaaa attaagaata ggagtttttc atatgactaa aacattacct gaagatttta
     6241 tttttggtgg agcaacagca gcatatcaag cagaaggtgc gacaaatacg gatggtaaag
     6301 gtcgcgtagc atgggatacg tatttggaag aaaactattg gtacactgca gaaccagcga
     6361 gtgattttta taacagatat ccagtggatt tagaattaag tgaaaaattc ggtgttaatg
     6421 gtatccgtat ttcaattgca tggtcacgta tcttcccaaa tggatatggc gaagttaacc
     6481 ctaaaggtgt tgagtattac cataagttat ttgcagagtg tcataaacgt catgttgaac
     6541 catttgtaac attgcatcat tttgatacac cagaagtgtt acacaaagat ggcgattttc
     6601 taaatcgtaa aacgattgac tactttgtag attatgctga atattgtttt aaagaattcc
     6661 cagaagtaaa atattggact acattcaatg aaattggacc tattggtgat ggtcaatatt
     6721 tagtaggtaa attccctcca ggtattaaat atgattttga aaaagttttc caatcacatc
     6781 acaatatgat ggttgctcat gcaagagcag taaaattatt taaagatggc ggctatcaag
     6841 gagaaattgg tgttgtacac gcattgccaa caaaatatcc gttcgatcca tcaaatcctg
     6901 aagatgttcg tgcagcagaa ttagaagata tcatccataa taaattcatc ttagatgcaa
     6961 catatttagg taagtattct cgtgaaacga tggaaggtgt gcaacatatc ttatctgtta
     7021 atggcggtaa attaaatatt acagatgaag attatgccat tttagatgca gccaaagatt
     7081 taaacgactt cttaggtatc aattactaca tgagtgactg gatgagaggt tacgatggag
     7141 aatctgaaat cacgcataat gcgacaggtg ataaaggcgg ttctaaatac caacttaaag
     7201 gtgtcggaca acgtgaattt gatgtcgacg tacctcgcac agactgggac tggatgatct
     7261 atcctcaagg tttatatgat caaatcatgc gtgttgttaa agattatcct aactatcaca
     7321 agatttatat cactgaaaat ggtttaggat ataaagatga atttattgaa tctgaaaaaa
     7381 cagttcatga tgatgcacgt attgattatg taagacaaca tttgaacgtt attgcagatg
     7441 ctattaaaga tggtgcaaat gttaaaggtt actttatttg gtcattaatg gatgtgttct
     7501 cttggtcaaa tggttatgaa aaacgatatg gtttattcta tgtcgatttt gagactcaag
     7561 aacgttatcc taagaaaagt gcttattggt acaaagagtt agccgaaaca aaagaaatta
     7621 aataattata gatattaaaa ggcctatcag tcgagtgctg gtaggtcttt atttatagaa
     7681 agagataaat taaacatgca tatacatatt tatatattaa tcaatgggtg acacaccatt
     7741 gaaagctaaa gaagcaatca attaacaaac gttaacggac aactttccaa agtcatctta
     7801 aaagttaatg atgtattcaa aatcaactaa aggacatttt taatcacatc ctttatactt
     7861 atataaaaga catgaatggg agttaaaagt gtgaagaaaa tttttgtaat tattacgaca
     7921 ttgttagcgg tcgctattat tataggctct attattacgg tggtgtttag tcaacgacag
     7981 gcacaaacat ttaaaataca acaacagcaa tttgttaaaa agccaattcc aacattgttc
     8041 ttacatggat ttggtggtag tgccaattct gaaaagttta tggtgaagca agcagaaaaa
     8101 agaggggtaa ctaaagatat catcactgct tacgtttcaa aggatggtgc agtgacattt
     8161 aaaggggaac taagaaaaga tgcagtgaat cctattgtaa aaattgaact tgaaaataac
     8221 agacaaggct atttggataa gaatgctgcg tggtttaaaa atgtactgac taagttacaa
     8281 tctgaataca atttcgataa attcaacttt gtcggtcact caatggggaa cttaacattt
     8341 gcacaatata tgatgacata tggtaacgac aagtcactgc cacagttaaa caaacaagtg
     8401 aatattgcag ggacatttaa tggtgtttta aatatgaatg aagatgtcaa tgaaattaca
     8461 gttgataaag atggtaaacc aagtcgaatg aatcaacctt atcagcaatt acgcgtgcta
     8521 aaagatattt ataaaggtaa aggtatcgaa gtgctaaata tttatggaga tttgaaagat
     8581 ggtacacatt cagacggcag agtgtctaat agttcttcga aatcattaaa atatttactg
     8641 ggaaatagtc cgaaaagtta tcgagaatct aaatatgaag gtgagcctgc acaacatagt
     8701 cagcttcatg agaatgaaaa tgttgctaat gagttaatcg acttcttatg gaaaaaataa
     8761 agcaactgct agttttctgg ggcaaaaaag cttgtaatat tagagctttt caaaaattat
     8821 ctattgtttg agagtttcca taaaatgtct aagtcgtaac attttaaatt tatgattata
     8881 gcaaatataa tgtggttgaa gttacatgaa tgaatcagtg cttaagtcaa aatataacaa
     8941 ctaagaatgt atttgcatac agacatcacg aagacacaat tcaaacttaa cgcttaagta
     9001 acaaataagt aaaagtttca aataaagaaa ggtgattaat gatgcaaaat aaacaaattc
     9061 tttttaacaa aataccagaa ggcatgcctc aagaggatac attcaaaatt gaagacattg
     9121 acacgcctaa acttgagagc gatggtgtac ttgtacaaat gctttatatt tcagtggatc
     9181 catatatgag aggacgtatg actaaggcag attcgtatgt acagcctttt gaaattggta
     9241 aaccgattgt tagccatgtc gttgcaaaag taattgattc gacactcgca gattataaaa
     9301 aaggagatgt cgttgtaggt atgttgcctt ggcgaatcat taatcatgtc caagcggatc
     9361 aaattactaa agttccaacg acagatgtac cacttgattt atatttaagt gttttaggaa
     9421 tgccaggaca aacagcatat catggtctgc ttgatattgg acaacctaaa gccggtgata
     9481 ctgttgtggt ttcagctgca tctggtgctg taggttcagt tgtaggtcaa attgctaaga
     9541 ttaaaggttg ccgtgtagta ggtattgcgg gtggcgacaa aaaagtaaat tatttaacag
     9601 agacactggg ctttgacgct ggtattgatt ataagaaaga ggattttgca gaagcgttag
     9661 cacaagcagt accagatggt attgatgttt attttgaaaa tgttggtgga acagttggcg
     9721 atgaagtatt taagcattta aatcgttttg cacgtgtacc agtatgtggc gcaatttcgt
     9781 catataatca tcctgaagca gatatcggac cacgtattca aggtacgctt attaagaagc
     9841 aagtaatgat gagaggcttc ttggtaagtg aattcgctaa tgcttttaaa gaagctagtg
     9901 aacagcttgc aacatgggtt caagaaggta aaattcagtc acaagtaacc attgaagatg
     9961 gttttgaaaa tgcgccacat gcatttaaaa atttatttac tggcgataac tttggtaaac
    10021 aagttattaa agtaacggaa taataaagta tacgttttaa caaactaact ttgttaattg
    10081 actaaaattt aataattttt tatctaaagc aaatttgttc agtaacatgg tagaaataag
    10141 tctaaaatgt aatacgaaag tcgtctgtaa ttatttatat attcaaatga aaaaggagtc
    10201 atgatgctgc gatataagca cacatggctt ctattttgta taataagaaa taagttcata
    10261 aatgaggtga aacgatatga aaatgatagg atttgagaag ccctttaaat tggaagaagg
    10321 aaatttattt aaagtatacg aacaaagaaa gccaacgcca gaaaacgatg atatcttagt
    10381 caaagtaaat agcattagtg taaatcctgt agatacaaag caacgacaaa tggaagtcac
    10441 tcaagcacct cgtgtacttg ggtttgatgc tattggtact gttgaagcta tcggaccaga
    10501 cgttacatta tttagtccgg gtgacgttgt attttatgct ggatcgccaa atagacaagg
    10561 atcaaatgcg acatatcaat tggtgtcaga agctatagtt gcaaaagcac cacacaacat
    10621 aagtgctaat gaagcggtta gtctaccgct aactggtatt acggcatatg aaacgttttt
    10681 tgatacattt aaaatttcac ataatccttc tgaaaatgta ggtaaatccg tgttaatcat
    10741 taatggcgct gggggtgtag gaagtattgc cactcaaatt gctaaacggt atggtttaac
    10801 tgtcattaca acagcatcac gacaagaaac aacagagtgg tgtgaaaaga tgggcgcaga
    10861 tatcgtattg aatcataaag aggatttagt gcgtcaattt aaagaaaaag aaattccatt
    10921 agtagattat atattttgca cctataatac agacttgtat tacaatacta tgattgaact
    10981 gataaaacca ttagggcata ttgcaactat cgtagctttt aatgaagatc aagatttaaa
    11041 tgcactaaaa ttaaaaagta taacatttac acatgaattt atgtttgcgc gtccaattca
    11101 tcgaacgcct gatatgatta aacaacatga gtatttagaa gatattacga aaaatataga
    11161 atcagggcat taccaaccaa caacgacaca agtttttgaa ggtttatcac ctgaaaactt
    11221 atatcaagca catcaattgt tggaaaaaca atcaatgatt ggaaaattag taattaatat
    11281 atagtttagt aagtaaagtc gccttatgca gaaaatactg tatagggcga cttatttttt
    11341 gtgtctagcg actattgatt ctaaatggaa atatggcaac tttgcaggta tcaaatcaat
    11401 aatgcactag aaaggcatta cttaaataca ataaaatact ttgttgctca atgataaata
    11461 atttgagtgt gttttatata tcatttttaa aagatgaggc attattaatt gtgtctatat
    11521 aaaattacaa atgattaaaa tgttgcagtt atgggtatga acttaccatt acacagaaag
    11581 ggtttgtatt tatgaaaaat aaatcagtaa gtctagtgtt ttgggtatct ttagtgatat
    11641 gtacgatatt tgttgctttt ggagcaatat ttccgaaaca gttagaaaaa ctcacgcaaa
    11701 acattactac atttattgcg ctacattttt cttggtatta tttattgctc gttttagtta
    11761 ttttatttgt ctgtgtgtat attttatttt cgagatacgc aaacattaca ttaggtgaag
    11821 aaggcgaaga tcctgaattc tcattgccgt cttggtttgc tatgttattt agtgctggta
    11881 tgggaatagg acttgtgttc tggacaacgg cagaaccaat cagtcatgcc ttcaaattaa
    11941 cgcctattca taaagctggc acgcaatctg caattaatga tgcaatgcag ttttcatttt
    12001 tccattgggg gattcatgca tgggcagtct atggtattgt cgcgcttgtg tttgcatatt
    12061 ttagctttca taaaggatat cctggcttag tcagcgctac attgacgcct ttattaggtg
    12121 aaaaagcaat gcgtggccct ttaggtggtg ccattgatgt attggcagtc atagctacag
    12181 taacaggcgt tgctgcaaca ttaggtttcg gtgcattgca aataaacgaa ggtttacatt
    12241 ttttatttaa tgtaccgagt aacttcacga tgcaagtaat cttgattgtc attgcaacca
    12301 tattatttac ttggtcagca tggtcaggta tcgataaagg tatcaaaacg ttgagtaata
    12361 tcaacatgtt acttgctttt gtagtactta ttggtctttt tattgttggt ccaacacttt
    12421 acattttaaa tacatttacg aatggtctat gtaattatat tgcaaatttc tttagtatga
    12481 gtttacgcat tccgtctggc ggtcaaaaat tccaatggtt acaaaattgg acaatctttt
    12541 actgggcatg gtggatatct tgggcgccat ttgtaggtat atttattgca cgtgtgtcaa
    12601 aaggacgtac gattaaagaa tttattctag gtgtactttt tgtcccggcg ttagtttgct
    12661 ttatattctt tgcggtattc ggcgcgtcag caatttactt acaagataat catattgccg
    12721 atattgctaa agctgcgaca gaaacagcaa cttttgcgac gctacaacat tatccgctag
    12781 gttttgtatt aagcttaatt actttacttg ttattatgat tttctttgtt acatcagcgg
    12841 attcggcaac ttatgtttta ggtatgttga gttcaagtgg tgatattaat ccgaagtcat
    12901 ttgtcaaagt gagttggggt atcattatgg cattatttgc aatcatcatg atttacacag
    12961 gtggtactca agcgattcaa aatttattaa tcattgctgc gttaccattt tccgtggtca
    13021 ttatagcgat gatatggtcg cttataaaat ctttgagtga ggagaaaccg agaaatagta
    13081 ataaggtttt aattaaacat cgtgacccag atattttaga atatcgttct tcaaaacata
    13141 ttgacgaaaa ttaattaata atgaggtttt atttacaaaa actagggaat atattactaa
    13201 atggtacttc atcttatata ttaaaaataa atttaaagtt attaatttat tatgtttaag
    13261 aaaaaaactg atgggtacat atttaatatg tttttaaatt aactttaact aaggaggtgt
    13321 cgttaggggt gaaacgactt aaaaacttta tccttggctt attaatagta gtaatcgtgg
    13381 ggttcctgtt attcatgtat atccaagatg gacgtataac tgaatatcaa gactacttcc
    13441 ttcaatttga atggttccaa ccattactta tttcacttgc tgcactattg atattaatag
    13501 gtcttatatt agtatttagt atcttcaaac ctacgcatcg aaaacctgga ttatacaaag
    13561 acttcgatga tggtcatgtt tacgtatctc gtaaagctgt tgaaaagacg gcattcgata
    13621 cggttgcaaa atatgatcaa gtaagacaac caaatgtagt tgcgaaacta tataacaaaa
    13681 aaaataaatc ttatatcgac attaaaactg actttttcgt accaaataat gttcaagttc
    13741 aatctttaac tgaagcaatt cgttcggata ttaaaaagaa tgtagaatat ttcacagaaa
    13801 tgccagtacg taagttagaa gttaatgtga gagatcagaa aacttctggt ccacgagtgt
    13861 tgtaagggag gtatatcatg gctaacaatc ataaccaaaa cggacaagac tctacacaac
    13921 aagtgattaa ttttttaaaa gtatttaaat ggagaatcgt tggtttccta gcgtttctat
    13981 taatcgcgat attattctta acgttaggtt tttggaaaac agtacttatc atcgttttat
    14041 gtttaattgg tgtaggtatt gggtatatga aagaccgtaa gcaagatttt atgaattttt
    14101 taaatcgatg gagttaattc atcaatttag aatttaatta aaaaaatata aaaattaaca
    14161 agtaaaagga gaatctatta tgactgtaga taacaataaa gcaaaacaag catacgacaa
    14221 tcaaactggt gttaacgaaa aagaaagaga agagcgtcaa aaacaacaag aacaaaatca
    14281 ggagcctcaa ttcaaaaaca aattaacatt ctctgatgaa gttgttgaaa aaattgctgg
    14341 tatcgctgca cgtgaagtta aaggtatctt agatatgaaa ggtggcttaa ctgatacatt
    14401 cactaatgca ttctcaagtg gcaacaatgt tactcaaggt gtatctgttg aagttggtga
    14461 aaaacaagct gctgtagact taaaagtaat cttagaatat ggtgaatcag caccaaaaat
    14521 cttccgtaaa gtaactgaat tagttaaaga acaagttaaa tatattactg gtttagatgt
    14581 agttgaagtt aacatgcaag ttgacgatgt aatgactcaa aaagagtgga aacaaaaaca
    14641 cgaaaaaaat aacgaaaaca ataaccaaga aagacaaggt ttacaataag ttattcgata
    14701 aaaagggctt gggacattaa attcttaggc
//
LOCUS       DAHHYJ010000061        14714 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000061 DAHHYJ010000000
VERSION     DAHHYJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14714
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.061"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..843
                     /locus_tag="NBK22_001411"
     CDS             <1..843
                     /locus_tag="NBK22_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD2414602.1"
                     /translation="MLSLFFFYSAFAVPFTFKNNVKKTATNLFILTMIICTIITYLLY
                     NNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKNRHINTLIAEQERHRIGQDLH
                     DTLGHVFASLSLKSELAYKLIDADVEKVKAELLAINKLSRESLNKVREIIDDVKLPSF
                     IEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITREAINNVIKHANASKVH
                     GKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHLNGTLAVDSTNGTKIIIEIST
                     GGIA"
     gene            840..1442
                     /locus_tag="NBK22_001412"
     CDS             840..1442
                     /locus_tag="NBK22_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2414603.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(1555..1743)
                     /locus_tag="NBK22_001413"
     CDS             complement(1555..1743)
                     /locus_tag="NBK22_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCD2414604.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            1882..2415
                     /locus_tag="NBK22_001414"
     CDS             1882..2415
                     /locus_tag="NBK22_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCD2414605.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            complement(2558..3412)
                     /locus_tag="NBK22_001415"
     CDS             complement(2558..3412)
                     /locus_tag="NBK22_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414606.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEISNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQNFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            3743..4318
                     /locus_tag="NBK22_001416"
     CDS             3743..4318
                     /locus_tag="NBK22_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414607.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(4372..5754)
                     /locus_tag="NBK22_001417"
     CDS             complement(4372..5754)
                     /locus_tag="NBK22_001417"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD2414608.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
     gene            5945..7225
                     /locus_tag="NBK22_001418"
     CDS             5945..7225
                     /locus_tag="NBK22_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCD2414609.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEI
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            7231..8292
                     /locus_tag="NBK22_001419"
     CDS             7231..8292
                     /locus_tag="NBK22_001419"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCD2414610.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            8294..9208
                     /locus_tag="NBK22_001420"
     CDS             8294..9208
                     /locus_tag="NBK22_001420"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCD2414611.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWE
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIAIQHNAYATVISGAGPTILIFSRKENSGELVR
                     SLNSQVVSCHSELVDINISGVKERIVYQ"
     gene            9266..10069
                     /locus_tag="NBK22_001421"
     CDS             9266..10069
                     /locus_tag="NBK22_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2414612.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYANEVTLDHNENGIPHALKKLL"
     gene            complement(10362..10676)
                     /locus_tag="NBK22_001422"
     CDS             complement(10362..10676)
                     /locus_tag="NBK22_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414613.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFMHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
     gene            complement(10651..10755)
                     /locus_tag="NBK22_001423"
     CDS             complement(10651..10755)
                     /locus_tag="NBK22_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414614.1"
                     /translation="MLPFFILDILHIVYNIDNNNFSGVKFNDWTNKFI"
     gene            complement(10894..12348)
                     /locus_tag="NBK22_001424"
     CDS             complement(10894..12348)
                     /locus_tag="NBK22_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2414615.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHTYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILALIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
     gene            12552..14069
                     /locus_tag="NBK22_001425"
     CDS             12552..14069
                     /locus_tag="NBK22_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCD2414616.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            14160..14309
                     /gene="rpmG"
                     /locus_tag="NBK22_001426"
     CDS             14160..14309
                     /gene="rpmG"
                     /locus_tag="NBK22_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD2414617.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
BASE COUNT         5312 a         2053 c         2570 g         4779 t
ORIGIN      
        1 atgctaagtt tgtttttctt ttatagtgct tttgccgttc catttacttt taaaaataat
       61 gttaaaaaaa cggcaactaa tcttttcata ctaacaatga ttatatgtac aataataacg
      121 tacttattgt ataacaacta ttttgttgca atgatggttt attatgtcgt tatatcgtta
      181 ataatgctag ataattttaa aaaaatgaaa aaccgtgaat atcaaaaaga aatagcagaa
      241 aaaaatagac atattaatac attaattgct gaacaagagc gacatagaat tggtcaagac
      301 ttacatgata cgttagggca tgtgtttgca agtttatcat taaaatcaga attagcttat
      361 aaactaatag atgctgatgt agaaaaagta aaagctgaat tattagcaat taataaatta
      421 tctcgtgaat cattgaacaa agttcgagaa attattgatg atgtaaaatt accatcattt
      481 attgaagaga ttgatagtat acgtaaagtt ttaaaagatg ctgatattga ttttacattt
      541 gaaaataaag aattagcgca agtattaagt cctactaaac aatctatgtt agttatgatt
      601 acgcgtgaag cgataaataa tgttattaaa catgcaaatg cttcaaaagt tcatggtaaa
      661 ttaaaaactg taaacaatca taaattactg cttatgattg aagatgatgg caaaggtatc
      721 gatagtgatt gtgaggtgaa aagtatttca cagcgtgtac aacatttaaa tggaacttta
      781 gcagtcgact caacaaatgg aactaaaata atcattgaaa tctcaacagg aggaatagca
      841 tgacatcttt aattattgca gaagatcaaa atatgttacg acaggcaatg gttcaattaa
      901 ttaaactaca tggtgatttt gaaattttag cagatactga taatggtctc gatgcaatga
      961 aacttattga agaatataat cctaacgttg ttattttaga tatagaaatg ccaggcatga
     1021 ctggacttga agttttagcg gaaattagaa aaaagcattt gaatattaaa gtgattattg
     1081 taacaacttt taaaagaccg ggatactttg aaaaagcagt tgtgaatgat gtggatgcat
     1141 atgttttaaa agaacgttct atagaagaat tggtggaaac cattaataaa gtaaataacg
     1201 gagagaaaga atatagcgcc acattgatga cttcattttt tgtagataaa aacccattaa
     1261 cgcccaaaga acaaattgta ttaagggaaa ttggcaatgg tttaagtagt aaagaaataa
     1321 gtgaaaaatt atttttgaca gatggaacag ttagaaatta tacatctgtt ataattgata
     1381 aattatttgc agataatcgt tttgatgctt ggaaaaaggc aaatgaaaaa ggctggatct
     1441 aaatacaaac aaaaaagtat tgagtgtttt gatttaagag acttggaaag tgagtcaata
     1501 atcaaaatct tactcaatac ttttattttg gtactcgcaa tttagatttg ttaactaatt
     1561 ttttcgctta ttataaaacg aaacaatcaa gtaaacgata aagcctacaa agatacccaa
     1621 taaaatagat agtactgccg tcactattaa tggtaatttg aaaaatattt gtaggaaaat
     1681 accaatgata attgcgataa ttactgcaat taatgtgact gtattttcat ttgaaatgtt
     1741 catttatttt cactccttaa caataacatt atatcatgct atagctttcc aaaatattga
     1801 aatatgtaga tatggctatt gacgatattt cttaactttt atatgattaa tcggaatgaa
     1861 aaaagagaag taggtggcaa tatgaagtca aataaatcgc ttgctatgat tgtggtagcc
     1921 atcattattg taggtgtatt agcatttcaa tttatgaatc atacgggtcc tttcaaaaag
     1981 gggacgaatc atgaaactgt acaagattta aatggtaaag ataaagtaca tgttcaaaga
     2041 gttgtggatg gtgatacatt tattgcaaat caaaatggta aagaaattaa agttaggctt
     2101 ataggggttg atacgccaga aacggtgaaa ccgaatacgc ctgtacaacc atttggcaaa
     2161 gaagcatcaa attatagtaa gaagacatta acaaatcaag atgtttattt agaatatgat
     2221 aaagaaaaac aagatcgcta tggtagaaca ttggcgtatg tatggataag taaagatcgt
     2281 atgtacaata aggaattagt ggaaaaggga cttgctagag agaagtattt ttcaccaaat
     2341 ggcaaatata gaaatgtatt tatagaagca caaaataaag ctaaacaaca gaaattaaat
     2401 atttggagta aataattaaa taggtgatta tatatattaa agtaaaatat acttgaagca
     2461 tcgatttatt attaccataa atttatatat agaaataatt taaaggcata ataaccatag
     2521 tgcgtatcga gcgtaatgga ttattatgcc tttgatttta cttgaatata gctgtgattt
     2581 gatcaacctg ttctttattt aaagggtgtt tagatagctc tatggcttcg ctaataatac
     2641 gtcgtaacga agattttgtt gaaactgtat caacatattt atctctttct tcagaacctt
     2701 gaatgatatt aactgtacca tcactaaagt aaataacacc ggtactttgg actttaaagt
     2761 taaagttttg ttcaatatga tcctttaatg ctttcgcatt atttgctgct aattgatacg
     2821 gatcataatc ataaaaatca taaataacac gatttggttg aactgtttct gtaaaagtat
     2881 agatagactt tcttgatgaa ttaaactgat catgatattg ctggctaatg taatgaccga
     2941 caactttttc aatattacta ttgcttattt ctgtatcatg ctcatcaggt acatcaaagt
     3001 gataaaatgt tttttcaccc cagcttttta catcaacgtt tattaaaccg atatctgaaa
     3061 caatgataaa atcaaatgaa cgtgcatact cgaaaaacgg atgtttagta gctaaattgc
     3121 ttgtaacgat aatatcgtaa tattttaatt taccattttc gagatagcta tctaaaatct
     3181 tgcgtaattc tcttttagca tttttgtatg catggtggcc aacatcatta ttagaactca
     3241 gcatttgact tcttaattca gcattttctg cacttaatgc tttgttcttt ttaataagtt
     3301 gctttcttgc ataaacttcg gtatctattt tactattact atacctttga tttaaaacta
     3361 atataccaat taatgctaca atgataatga taagtacaac ataaaaagac attttttcac
     3421 caatcctttt tgacttcttt aactttgtat acaataataa ttaataaaga ttaattgtta
     3481 ttcaatttcc cacattttta ttagttgatt ttagttcatc attgttataa tcaaattata
     3541 aactgacaga tattgatgtt caatgaatat gacgtgaaag attcgtgaat tcaagtttat
     3601 gtcgaattta tgttataacg gtcatttaaa tgacagaatt aggtcactca tagtattttg
     3661 aagattgaat tcattaattt taaaatgtat aatgatattt gtgaaagcgc ttgcttagga
     3721 ggtgtatttg agagtgaatg aaatgaatgc taaagaacaa ttagtggaca atttaatgaa
     3781 aacatcatcg caattattta aatttcacgg tgaagttgcc atgcagcttt tcttaaatga
     3841 tgaattaaaa ttaccttcta ttgttgaaat atgcgtggaa cgtaagcgtt taagtgatat
     3901 tgtgaaagtt attccgcaat catatgcgtt actatacata gataagcaag atcaagcaat
     3961 agctaaagaa gatttatcac tttcaaaaat tgcaaaagtt tatgtgcaat atgatgatac
     4021 aacaataatg agtattttcg tttatgatgt agtaaacgat gaatggattt ttagattgga
     4081 tccgaatata cgtataccta agagtaacat atacttccat agtttaaatt gggatgtgga
     4141 ttatattaaa ccggagatcg ttctaatgta tgatctaatg caacaccatc agtatcatca
     4201 ttattccaat tataaacgag tcatagatgc attaagctac tatcaatttt ttattttaaa
     4261 atttgtagta ggtgagcaac gtattaagga tgcaatccag agaacaataa ataattaaga
     4321 aaaagcaatt cataacgcag ttgaatacat gtgttacgaa ttgcttttat attagttttt
     4381 atcacacaag ttttttaatg caaccccgtg atagcaaaac tcatatgtag ataatacagc
     4441 tttttcagca tcatctacat gaattccaaa catcatcgag atttccgaag caccttggtt
     4501 aatcattttt aagttaattt ttgattcagc taaggcatgt gtaattttat ttgcagtacc
     4561 gatgacttta ttcatacctt cgccgacaat cattaatatt gctaaatcat gctcaatact
     4621 tagctcatca acatcacatt tttgacgaat ttcatttaat acttttgttt ctttattttg
     4681 aatttgtttt gaacgcataa cgatactgat agtatcaata cctgaaggca tatgatcaaa
     4741 tgaaatatta ttatcctcta agacacctaa tatctttcta gtaaacccga cttgtctatt
     4801 cattaaatac tttttgatat taataacggt aaaatcttta tcacaactta taccgctaat
     4861 cacatttttc gcatttattt ctctatcatg cactataaat gtacctttat cttgagggcg
     4921 gttcgtattt ttaatcacca cagggatgcg atctttataa agtggttgta aggcttcatc
     4981 atggaaaaca ctaaaaccag cataagataa ttcacgcatt tctcgataag tgatttcttc
     5041 gattaattca ggatctttga tgatatttgg attagcttta taaataccag acacatcggt
     5101 gaaattttca taaattgtag ctctaacacc acttgatatg atggcgccag ttatatctga
     5161 tccgccacgt ggaaatgtaa ctatatatcc ttcatgagat acgccgaaaa atcctgggat
     5221 aattagtttc tcatcataat ctcttaattt tttaatttca gagtaagcac tatctaatat
     5281 ttgtgcttct tgtgggacgt cagtaacaaa aatacccgct tccttcggtg atatatattt
     5341 tgttggtata ccttgactat tattatataa agctatcaat tgtgcattaa aatcttcacc
     5401 acaagaaagt aatgcatcta atagtctctt tggttcattt tttaattgat taatataatg
     5461 ttccaaagtc acatctatcg tccgtaaaat actttcatcc atttgcaatt cttttacaat
     5521 atcatcataa cgctgaataa tttctctttt tttatcatga taatcaagat gattaatgac
     5581 cttttcatat aatctgatta acaaatcagt tgttttaata tcattatcat gtcttttacc
     5641 tggagcagaa acgataacaa tctttcgctc tggatcagaa ttaacaatat ttaaaacctt
     5701 tttaatttga gtagcattgg agacggagct accaccgaat ttggaaactt tcatagtgca
     5761 tgccaacctt tctaaaaaat cagaaaattg tattcagttt tgtaagtaag aatgatatta
     5821 tgtatttata ttttgaatat tacgaacttt acaaaaataa agaataattc tatactatac
     5881 tatcatctag tgtttttcaa taatacattt gtttttctca aaagcacaaa cggaggtaca
     5941 taaaatgaaa aaattaaata tagcattatt aggattaggt actgtcggat ctggtgttgt
     6001 taaaatcatc gaagagaacc gacagcaaat tcaagataca ttaaataaag atattgtcat
     6061 aaagcatatt cttgttcgag ataaatctaa aaagagaccg ctaaatatta gccaatatca
     6121 tttaactgaa gatgttaatg aaattttaaa tgatgattca ttagatatta tcgttgaagt
     6181 catgggagga attgaaccaa ctgtagattg gttaagaaca gcacttaaaa ataaaaaaca
     6241 tgttattacc gcaaataaag atttattagc agtacatctt aaacttttag aagatttagc
     6301 agaagaaaat ggtgtagctt taaagtttga agcgagtgta gcaggtggta ttccgatcgt
     6361 aaatgccata aataatggtt tgaatgcgaa taatatttca aaatttatgg gaattttaaa
     6421 tggtacctct aattttattt tatctaaaat gactaaagag caaacgacat ttgaggaagc
     6481 acttgatgaa gcgaaaagac ttggttttgc tgaagcggat ccaactgatg atgtagaagg
     6541 ggtagatgca gcgcgtaaag ttgtcattac atcatattta tcatttaacc aagtcattaa
     6601 attaaacgac gttaaacgaa gaggaattag tggcgtaact ttaactgata ttaatgtagc
     6661 cgatcaactg gggtataaaa ttaaattgat tggtaaggga atatatgaaa atggcaaagt
     6721 taatgcatcg gtagaaccaa cgttaattga taaaaagcat caattagcag ctgtagagga
     6781 tgaatataac gcgatttatg tcattggtga tgccgttggt gacacgatgt tttatggaaa
     6841 aggagcaggc agtttagcaa caggtagtgc cgttgtcagt gatttattga atgtagcatt
     6901 attctttgaa tcagatttac acacattgcc accacatttt gaaataaaga cagataaaac
     6961 acgggaaatg atggattcag atgcagaaat taatattaaa gaaaaatcca atttctttgt
     7021 agtagtgaat catgtcaaag gttcaattga aaattttgaa aatgagttaa aggcaatatt
     7081 accatttcac cgatcattaa gagttgcaaa ttacgataat caatcatatg ccgctgttat
     7141 agttggattg gaatcatcac cggaagaatt aatcactaag catggatacg aagttgacaa
     7201 agtataccca gtagaaggag tttaattata atgagaagat ggcaaggatt agtagaagag
     7261 tttaaagcac atttaccagt aaatgaaaat acaccaaaat taacattgaa cgagggaaat
     7321 acaccactca ttcattgtga aaatatgtct aaaatactag gtatagattt atatgtgaag
     7381 tatgaaggtg ccaatccgac aggttcattt aaagatcgcg gtatggtaat ggctgtgaca
     7441 aaagcaaaag agcaaggtaa gaaaattgta atatgcgctt cgactggaaa tacatcagcg
     7501 tctgcagcag catatgcagc gagagcaggt ttaaaagcta tcgtcgtaat accagaaggt
     7561 aaaattgcat taggtaaatt gtcgcaagca gtaatgtatg gtgcagaaat cgtttctatt
     7621 gaaggaaact ttgatgaagc tttagaaatt gtaaaagaaa ttgcaaaaag tggcgaaata
     7681 gagcttgtaa actctgtcaa tccatttaga atcgaagggc aaaagacagg ctcatttgaa
     7741 attgtacaac aattagacgg tgaagcacct gatattttag cgattcctgt aggtaatgca
     7801 ggtaatatta ctgcatattg gaaaggcttt aaagaatatc atgaagctaa aggttcacaa
     7861 ttgccgaaaa tgtttggctt ccaagctgaa ggcgcatcac caattgttca aaataaagtc
     7921 attaaaaatc ctgaaacgat tgcaactgct attcgaattg gtaatcctgc tagttgggat
     7981 aaggcgacta atgctcttaa agaatcaaat ggattaatag atagtgttac tgatgatgaa
     8041 attctagaag catatcagtt aatgacaact aaagaaggtg tctttagtga accagcgagt
     8101 aatgcttcta ttgcaggttt aattaaattg catagacaag gtaaattacc tcaaggtaaa
     8161 aaagtagttg ctattttaac tggtaatgga ttaaaagatc ctgatactgc tatttcacta
     8221 ctagataatc cgataaagcc attgccaaat gataaagata gcattatcga ttatattaaa
     8281 ggagctttat aacatgtcga atgttttgga gttaacaatt cctgcatcaa cagccaacct
     8341 tggagttggc tttgattcta taggtatggc tttagataaa tttttgcatc tgtctgtaaa
     8401 ggaaacatca gggacaaaat gggaatatat tttccatgat gatgcatcta agcaattgcc
     8461 tactgacgaa acaaacttta tttatcatgt agcacaacaa gttgcttcta aatatagtgt
     8521 tgacttgcct aatttatgta tcgaaatgag aagtgatatt ccattggcaa gagggttagg
     8581 ttcgtcagct tctgctttag taggagctat atatatcgca aattattttg gtgatatcca
     8641 actgtctaaa catgaggtat tacaattagc gactgaaatc gaaggacatc ctgataatgt
     8701 tgcgccgacc atttatggtg gtttaatcgc tggatattat aatgatgtct cgaaagaaac
     8761 gtcagttgca catatcgaca taccagacgt ggatgtgatt gtaacgatac caacttatga
     8821 actaaaaaca gaagcatcaa gacgtgcttt accacaaaaa ttaacacata gtgaagcggt
     8881 taaaagtagt gcaattagta atacaatgat ttgtgcatta gcacagcaca attatgaatt
     8941 agcaggtaaa ctcatgcaac aagatggctt tcatgaaccg tatcgtcagc atttaattgc
     9001 tgaatttgat gaagtgaaaa caattgctat tcaacataat gcctatgcaa ctgtaattag
     9061 tggtgctgga ccaactattt taatatttag tcgtaaagaa aatagtgggg aattggttcg
     9121 ctctttaaat agtcaggtag tatcatgcca ttctgaattg gtcgatatta atatcagtgg
     9181 tgttaaagaa cgaattgtat accaatagac gctttatatt gtaaaatagt attaaatgca
     9241 gaatagagag gagatttaat gcgatatgac aaattataaa gttgtcgttt tagatatgga
     9301 tgacacattg ctaaattcag ataatgtgat atcagaagaa actgcaaatt atttaacagc
     9361 aattcaagat gaaggttatt atgttgtttt agcatctggt agacctactg aaggtatgat
     9421 tccaactgct agagatttaa aattacctga acatcatagc tatattatta gttataacgg
     9481 tagtaaaacg attaacatga ctaatgaaga agtagaagta agtaaatcga ttggtaagca
     9541 agatttcgat gaaattgtag attattgtcg agatagaggc tttttcgttc ttacatatca
     9601 tgatggtcaa attatttacg acagcgaaca tgagtatatg aatattgaag cagaattaac
     9661 aggtttaccg atgaaacgtg ttgatgatat caaagcgtat attcaaggcg atgtacccaa
     9721 ggtcatgggt gtagattatg tagcgaatat tacagaagct agaattgatt tgaatggtgt
     9781 gttcaatgat aatgtagatg ctacgacaag taagccattc ttcttagaat ttatggccaa
     9841 agacgtttca aaaggtaatg caattaaagc gttatgtcac aaattgggat attcggtgga
     9901 tcaagtcatt gcttttggtg atagtatgaa tgataaatca atgtttgaag tcgcaggtct
     9961 agctattgct atggggaatg catcagatga acttaagcaa tatgcaaatg aagttacgtt
    10021 ggatcataat gaaaatggta ttccacatgc gctcaaaaaa ttgttataaa ttttaaaata
    10081 agccttaaca catgatattt gaataagata tcttgtggtt aaggcttttt atttttgtga
    10141 aaatgacttc agttatacta tggaggattt gaaatacata ttttagatta gtaatgatat
    10201 caaacgaata gagtaaatgt atattttttg aataaatcaa gtattaacta gtcactgaag
    10261 gtagataaat aaatttgatc attctgaata ataacgaatg cattaaaaaa tgcagtgaca
    10321 atctaacatt aattgttaaa atcactgcaa gcatcttgat tttatttagc tgtttgtgtc
    10381 cagtcatgct tacctctgaa acgttttaca tgagcatata tttggtcagc agtataatct
    10441 aaatttgtat aaactgtaat actgaacgct gattggtttt cagcaaatga ttcttcggaa
    10501 aataattcct ttaagtgcat aaataaatca ttcatttgtt tatggtttaa accgggatat
    10561 tcccttaaag ttctctttaa taattgtccg ttcttttctt ctaatgcttg aactacaata
    10621 acaaatcttt catcttgctt tagtacatca tcaaataaat ttgtttgtcc aatcattgaa
    10681 tttcacccct gaaaagttat tgttatctat attatacacg atatgcaaaa tatctaaaat
    10741 aaagaatggt aacatggtaa taataaaata taaacagaat actagttaat acactttata
    10801 ctagaaaaag atattaaagt atatctgctt tacaccatta aaaagcggca aatgctatag
    10861 ataatctaaa gcatctgccg tatgagaatt tatttatttt tgattgtcat aatcgtgtgg
    10921 ttttaaatta attgtttcta gctttacaaa ttttgttttg tgaatgattt tatgaataaa
    10981 gtaaatcaac gctagaatga ttaaaggtaa aaagttttta aaagcattta accattgatc
    11041 ttttaaaata tattcaactg agccaccaaa tagcaagaat aatagtgtag tgatgacaat
    11101 gattggtcct aatggataaa aaggtgcttt atatggtagg accttattag ggtcttgacc
    11161 ttgtttttta atagcttgtc gcaatcgtat ttgtgaccaa atgcttgatc cccaaacaac
    11221 tataatcatt gaaccaataa tttcaagtaa attaaaaacg gcatttgaat taaagtttgc
    11281 ataaataata acaataacaa cgactgcata agtagttaat aatgctctta aaggtaactt
    11341 agttgtcttg tttaatttac ttaaaaattg gggtgctttt ttgtctgaac ttaaggaata
    11401 caacattctg cctgttgtat aaacacctga atttgcagcg gataataatg aagttaaaat
    11461 aaccgcgttg attactgatg ctgcaaaggc tatgcctact ctatcgaata caattgtaaa
    11521 tgggctttga cttattgaac tacttgctct taataatgat ggatctgtgt acggaataat
    11581 tgcaccaatt actgcaattg ataagacata gaataaaaga atacgccaaa atacttgttt
    11641 aattgcctta ggcatagact ttttagggtc atctgattca ccagcagtta ctgctactac
    11701 ttctgtacca ccaaccgaaa atccggcgac taataatacg cctaagaaac cagagatacc
    11761 accaacaaac ggtgcttggc cttttgtata gttttcaaat ccatatgtat gaccacctaa
    11821 gataccgaaa atcattaaaa agccaaaaat aacgaatacg ataattgtta acactttaat
    11881 caatgataac caaaactcag tttctccaaa tgattttaca gaaaaaatgt ttaataatag
    11941 taaaattgta ataaagatta agctccaagt aatggggtgg aaaaatttaa atgtgtccca
    12001 gaaataaagc acatttgacg ctactatgac atcaacactt gtaactaatg accacaatgc
    12061 ccaatacaac catcccatgg taaagccaag agatgagtca ataaagcgtg ttgaataaga
    12121 gctgaatgaa cctgatactg gataaaatgt tgccaactct ccaattgatg acattaagaa
    12181 atatagcatg acaccaataa caagataagc gagtatagcg cctccaggac cagcttgaga
    12241 aatgatatta ccagtagcta caaatagacc agtcccaatt gcaccaccta tagcaatcat
    12301 ggaaatgtgt cttgagttaa gactacggtt cattttatta tcttccatat ttagtctccc
    12361 atctatttaa atatacccat tattgtaagc tttttaagtg tactattcaa taactattta
    12421 gtactgtaaa gcgaaaaaat taaaattttc tgatttttta atcatcttga gcatgtttaa
    12481 ttgtaatttt gatggggtta aattataata tgtattaaat tataattatt ataaattgtg
    12541 gagggatgac tatgtcacaa caagacaaaa agttaactgg tgtttttggg catccagtat
    12601 cagaccgaga aaatagtatg acagcagggc ctaggggacc tcttttaatg caagatattt
    12661 actttttaga gcaaatgtct caatttgata gagaagtaat accagaacgt cgaatgcatg
    12721 ccaaaggttc tggtgcattt gggacattta ctgtaactaa agatataaca aaatatacga
    12781 atgctaaaat attctctgaa ataggtaagc aaaccgaaat gtttgcccgt ttctctactg
    12841 tagcaggaga acgtggtgct gctgatgcgg agcgtgacat tcgaggattt gcgttaaagt
    12901 tctacactga agaagggaac tgggatttag tagggaataa cacaccagta ttcttcttta
    12961 gagatccaaa gttatttgtt agtttaaatc gtgcggtgaa acgagatcct agaacaaata
    13021 tgagagatgc acaaaataac tgggatttct ggacgggtct tccagaagca ttgcaccaag
    13081 taacgatctt aatgtcagat agagggattc ctaaagattt acgtcatatg catgggttcg
    13141 gttctcacac atactctatg tataatgatt ctggtgaacg tgtttgggtt aaattccatt
    13201 ttagaacgca acaaggtatt gaaaacttaa ctgatgaaga agctgctgaa attatagcta
    13261 cagatcgtga ttcatctcaa cgcgatttat tcgaagccat tgaaaaaggt gattatccaa
    13321 aatggacaat gtatattcaa gtaatgactg aggaacaagc taaaaaccat aaagataatc
    13381 catttgattt aacaaaagta tggtatcacg atgagtatcc tctaattgaa gttggagagt
    13441 ttgaattaaa tagaaatcca gataattact ttatggatgt tgaacaagct gcgtttgcac
    13501 caactaatat tattccagga ttagattttt ctccagacaa aatgctgcaa gggcgtttat
    13561 tctcatatgg cgatgcgcaa agatatcgat taggagttaa tcattggcag attcctgtaa
    13621 accaacctaa aggtgttggt attgaaaata tttgtccttt tagtagagat ggtcaaatgc
    13681 gcgtagttga caataatcaa ggtggaggaa cacattatta tccaaataac catggtaaat
    13741 ttgattctca acctgaatat aaaaagccac cattcccaac tgatggatat ggctatgaat
    13801 ataatcaacg tcaagatgat gataattatt ttgaacaacc aggtaaattg tttagattac
    13861 aatcagagga cgctaaagaa agaattttta caaatacagc aaatgcaatg gaaggcgtaa
    13921 cggatgatgt taaacgacgt catattcgtc attgttacaa agctgaccca gaatatggta
    13981 aaggtgttgc aaaagcatta ggtattgata taaattctat tgatcttgaa actgaaaatg
    14041 atgaaacata cgaaaacttt gaaaaataaa tttgatatgt agtttctata ttgcgtagtt
    14101 gagcagttta tgatatcata ataaatcgta aagattccta acaagagagg gtgtttaacg
    14161 tgcgcgtaaa cgtaacatta gcatgcacag aatgtggcga tcgtaactat atcactacta
    14221 aaaataaacg taataatcct gagcgtattg aaatgaaaaa atattgccca agattaaaca
    14281 aatatacgtt acatcgtgaa actaagtaat tcttatcatt caaatacgac gatttgaaaa
    14341 taaagcgggc ttacctatta tattggggag ctcgcttttt tatgaaattt ttgtgaagag
    14401 tgattaatgg attgagtttc atcggtagaa caatatatga ttgtattagt tgttacttta
    14461 ttaaaatttg agaatattta tagaaggaaa tagattactg attttataaa gtcactttgt
    14521 tagcgaatgc ttgaaagagt atttaatata gtagaattta aaatttcaaa gcggaattta
    14581 ataagtacga agtaattctg ggtatgtttt ataaatgttc gataatacac tttaatctta
    14641 aatatgatgg tttagaaaat gatttaacaa agaaatgaaa ctttactgtt gaattatgtg
    14701 aggattgtgt tatt
//
LOCUS       DAHHYJ010000062        14636 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000062 DAHHYJ010000000
VERSION     DAHHYJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.062"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            241..2664
                     /locus_tag="NBK22_001427"
     CDS             241..2664
                     /locus_tag="NBK22_001427"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCD2414618.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEAKGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDE
                     YKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYG
                     TGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEA
                     AITKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPL
                     LLPETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYID
                     PKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKL
                     FNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLD
                     GSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQ
                     LMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEA
                     LLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVP
                     QKLVNIVAK"
     gene            2686..2997
                     /locus_tag="NBK22_001428"
     CDS             2686..2997
                     /locus_tag="NBK22_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD2414619.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLIPMD
                     TIPDNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMHAWGDEGLEIKS
                     I"
     gene            3323..9883
                     /gene="sasC"
                     /locus_tag="NBK22_001429"
     CDS             3323..9883
                     /gene="sasC"
                     /locus_tag="NBK22_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCD2414620.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDNNV
                     QSDTNQATPVNSQDKDVANNRGLANSAQNTPNQSATTNQSTNQALVNHNNGGIVNQAT
                     PTSVQSSTPSAQNNNHTDGNTTATETVLNANNNDVVSNNTTLNVPNKTNENGSGGHLT
                     LKEIQEDVRHSSDKPELVAIAEPASNRPKKRSRRAAPADPNATPADPAAAAAGNGGAP
                     VAITAPYTPTTDPNANNAGQNAPNEVLSFDDNGIRPSTNRSVPTVNVVNNLPGFTLIN
                     GGKVGVFSHAMVRTSMFDSGDNKNYQAQGNVIALGRILGTDTNDHGDFNGIEKALTVN
                     PNSELIFEFNTMTTKNGQGATNVIIKNADTNDTIAEKTVEGGPTLRLFKVPDNVRNLK
                     IQFVPKNDAITDARGIYQLKDGYKYYSFVDSIGLHSGSHVYVERRTMDPTATNNKEFT
                     VTTSLKNNGNSGASLDTNDFVYQVQLPEGVEYVNNSLTKDFPSNNSGVDVNDMNVTYD
                     AANRVITIKSTGGGTANSPARLMPDKILDLRYKLRVNNVPTPRTVTFNETLTYKTYTQ
                     DFINSPAESHTVSTNPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKKRAQT
                     ILAENRNNVPLNKRVSQADIDTLTNQMQHTLIRSVDAENAVNQKADQMEDLVNQNDEL
                     TDEEKQAAIQVIEEHKGNIIGDIGDQTTDDGVTRIKDQGIQTLSGDTATPVVKPNAKK
                     AIRDKATKQREIINATPDATEDEIQDAINQLATDETDAIDNVTNATTNADVETAKNNG
                     INTIGAVVPQVTHKKAARDAINQATATKRQQINSNREATQEEKNAALNELTQATNHAL
                     EQINQATTNADVDNAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANPDAT
                     QEERQAAIDKVNAAVTAANTNILNANTNAEVEQVKTNAIQGIQAITPATKVKTDAKNA
                     IDKSAEKQHNTIYNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKDQGT
                     QNIVVIQPATQVKTDARNVVNDKAREAITNINATPGATREEKQEAINRVNTLKNRALT
                     DIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTNATT
                     EEKQVALNQVDQELATAINNINQADTNAEVDQAQQLGTKAINAIQPNIVKKTAALAQI
                     NQHYKAKLAEINVTPDATNDEKNAAINTLNQDRQQAIESIKQANTNAEVDQAATVAEN
                     NIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQAINQ
                     INQNQTNDQVDTTTNQAVNAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSLDSTDN
                     EKEVASQALAKEKEKALAAIDQAQTNSQVNQAVTNGVSAIKIIQPETKVKPAAREKIN
                     QKANELRAKINQDKEATAEERQVALDKINEFVNQAMTDITNNRTNQQVDDTTSQALDS
                     IALVAPEHIVRAAARDAVKQQYEAKKQEIEQAEHATDEEKQVALNQLANNEKRALQNI
                     DQAIANNDVKRVESNGIATLKGVEPHIVVKPEAQQAIKASAENQVESIKDTPHATVDE
                     LDEANQLISDTLKQAQQEIENTNQDAAVTDVRNQTIKAIEQIKPKVRRKRAALDSIEE
                     NNKNQLDAIRNTLDTTQDERDVAIDTLNKIVNTIKNDIAQNKTNAEVDRTETDGNDNI
                     KVILPKVQVKPAARQSVGVKAEAQNALIDQSDLSTEEERLAAKHLVEQALNQAIDQIN
                     HADKTAQVNQDSINAQNIISKIKPATTVKATALQQIQNIATNKINLIKANNEATDEEQ
                     NIAIAQVEKELIKAKQQIASAVTNADVAYLLHDEKNEIREIEPVINRKASAREQLTTL
                     LNDKKQAIEANVQATVEERNSVLAELQNIYDAAIGQLDHDRSNAQVDKTASFNLQTIH
                     DLDVHPVKKPDAEKTINVDLVRVTALVQNYRKVSDRNKVDALKAITALKLQMDEELKT
                     ARTNADVDAVLKRFNVALSDIEAVITEKENSLLRIDNIAQQTYAKFKAIATPEQLAKI
                     KVLIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEATKVSPKVIQPAPKV
                     CTPIKKEETHESRKVEKELPNTGSEGMDLPLKEFALITGAALLARRRTKNEKES"
     gene            complement(10000..11268)
                     /locus_tag="NBK22_001430"
     CDS             complement(10000..11268)
                     /locus_tag="NBK22_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD2414621.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSSVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVNTDQTFTVHTQNNSYESHSLVIA
                     TGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNGKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYSHTSME"
     gene            11675..13336
                     /locus_tag="NBK22_001431"
     CDS             11675..13336
                     /locus_tag="NBK22_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD2414622.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPFITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFCYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            13333..14028
                     /locus_tag="NBK22_001432"
     CDS             13333..14028
                     /locus_tag="NBK22_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD2414623.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGLVNVNEQVIKSPKTHIEPE
                     NDKITVRGELIEYVENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGVTLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            14045..14467
                     /locus_tag="NBK22_001433"
     CDS             14045..14467
                     /locus_tag="NBK22_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD2414624.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLRAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGAAPNNNLRIVTEEDLKKNKNALSDKE"
BASE COUNT         5556 a         2236 c         2678 g         4166 t
ORIGIN      
        1 acataatatt aatttgtata atttaatttc gtttggagct tttctacaga aagctagtga
       61 tgctgagagc tagtgttaag gactaaatgt aaatcgtatt aattttaaat tgaatgaatg
      121 acatctctta ctattaaaat gagtgcacaa tttttgtgaa atagggtggt aacgcggcaa
      181 atgtcgtccc tatgtaaata gaatagttag aggtgtcttt tttattgaat aggaggaaat
      241 gtgttgaatt acaaccacaa tcaaattgaa aagaaatggc aagactattg ggacgaaaat
      301 aaaacattta aaacaaatga taacttaggt caaaagaaat tttatgcttt agacatgttt
      361 ccatatccat caggtgctgg tttacatgtt ggacatcctg agggctatac agcaacagat
      421 atcatttcaa gatataaaag aatgcaagga tataatgtat tacatccgat ggggtgggat
      481 gcattcggat taccagcaga gcaatatgct ttagacactg gcaacgaccc acgtgaattt
      541 acaaagaaaa atatccaaac ttttaaacga caaattaaag aattagggtt cagttatgat
      601 tgggatcgtg aagttaatac aacagatcca gaatactata aatggacaca gtggattttc
      661 atacagttat ataacaaagg tttagcatac gttgatgaag ttgcagttaa ctggtgtcca
      721 gcattaggca ctgttttatc taacgaagaa gtgattgatg gtgtctctga acgtggtgga
      781 catccagttt atcgtaagcc gatgaaacaa tgggtactta aaatcacaga atatgcagat
      841 caattattag cagatttaga tgatttagat tggcctgaat ctttaaaaga tatgcagcgc
      901 aattggattg gacgttctga aggggccaaa gtttcatttg atgtagataa tacggaagcg
      961 aaaggaaaag tagaagtatt tacgactaga ccagatacaa tctatggtgc atcattctta
     1021 gtcttaagtc ctgaacatgc attagttaat tcaattacaa ctgatgaata taaagaaaaa
     1081 gtaaaagctt atcaaacaga agcttctaaa aagtcagatt tagaacgtac agatttagca
     1141 aaagataaat caggtgtgtt tactggtgca tatgcaatta atcctttatc tggtgaaaaa
     1201 gtacaaattt ggattgctga ttatgtatta tcaacatatg gtactggagc aattatggca
     1261 gtaccagcgc atgatgacag agattatgaa tttgctaaaa agtttgattt gccaatcatt
     1321 gaagtcatcg aaggtggaaa tgttgaagaa gcagcataca ctggtgaagg taaacatatt
     1381 aattctggtg aacttgatgg tttagaaaat gaagcggcaa ttactaaagc tattcaatta
     1441 ttagagcaaa aaggtgctgg cgaaaagaaa gttaattaca aattaagaga ttggttattc
     1501 agtcgtcagc gttattgggg cgaaccaatt cctgtcattc attgggaaga tggaacaatg
     1561 acaactgttc ctgaagaaga gctaccattg ttgttacctg aaacagatga aatcaagcca
     1621 tcagggactg gtgagtctcc actagctaat attgattcat ttgtaaatgt tgtagatgaa
     1681 aaaacaggta tgaaaggacg tcgtgaaaca aatacaatgc cacaatgggc aggtagttgt
     1741 tggtattatt tacgttacat cgatcctaaa aatgaaaata tgttagcaga tcctgaaaaa
     1801 ttaaaacatt ggttacctgt tgatttatat atcggtggag tagaacatgc ggttcttcac
     1861 ttattatatg caagattttg gcataaagtc ctttatgatt tgggtatcgt acctactaaa
     1921 gaacctttcc aaaaattatt taaccaaggt atgattttag gagaaggtaa tgagaagatg
     1981 agtaaatcta aaggaaatgt aatcaatcct gatgatatag tacagtctca tggtgcagat
     2041 actttacgtc tttacgaaat gtttatggga cctttagatg ctgcaattgc atggagtgaa
     2101 aaaggattag atgggtctcg tcgattctta gatcgcgtat ggcgtttaat ggtaaatgaa
     2161 gatggaacat tgagttcaaa aattgtaact acaaataata aatctttaga taaagtttat
     2221 aaccaaactg ttaaaaaggt aacagaagac tttgaaacat taggatttaa tactgctatt
     2281 agtcaattaa tggtatttat taatgagtgt tataaagttg atgaagttta taaaccttac
     2341 attgaaggct tcgttaaaat gttagcacct attgcaccac atatcggtga agaattatgg
     2401 tcaaaattag gacatgaaga gtctattacg taccaacctt ggccaactta tgacgaagca
     2461 ctacttgtag atgatgaagt agaaatcgtt gttcaagtga atggtaaatt gagagctaaa
     2521 attaaaattg ctaaagatac atcaaaagaa gaaatgcaag aaattgcctt atctaatgac
     2581 aatgttaaag cgagtattga aggtaaagac atcatgaaag tcatcgctgt tcctcaaaaa
     2641 ttagtcaata ttgtagctaa ataatgtttt aaggaggact ttgaaatgaa gtcaattact
     2701 acagatgaat taaaaaataa acttttagaa tctaaaccag ttcaaattgt tgatgttcgt
     2761 actgatgaag aaacagcaat gggatatatt cctaatgcaa agttaattcc aatggatacc
     2821 attccggata atttaaattc atttaataaa aatgaaatat attatattgt atgtgctggt
     2881 ggagttcgaa gcgctaaagt tgtagaatat ttagaggcaa atggcattga tgccgtaaat
     2941 gtcgaaggcg gcatgcacgc atggggcgat gaaggtttgg aaataaaaag tatttaaagt
     3001 agtgacataa tttaaaataa tattacattt gtaatgacac caagtaacgt ttcggttgct
     3061 tggtgttttt tggtatgaat tactttctgt tacaaaacaa tctaaagcgt tcttgttatg
     3121 tattattaag attttaatta caaaacggaa actaaattgt aataaaataa aactttattt
     3181 tataaaatga tgatgataaa attgagtgaa cttaaaatat tgtacaaaat aatatagcta
     3241 taaacataat atgagggagc gtatattttt agcataattc ttaacaacac agcagagaac
     3301 agacaaccag gaggaaaatg aaatgaattt gttaaagaaa aataaatata gtattaggaa
     3361 gtataaagta ggcatattct ctactttaat cggaacagtt ttattacttt caaacccaaa
     3421 tggtgcacaa gccttaacta cggataataa tgtacaaagc gatactaatc aagcaacacc
     3481 tgtaaattca caagataaag atgttgctaa taatagaggt ttagcaaata gtgcgcagaa
     3541 tacacctaat caatctgcaa caaccaatca atcaacgaat caagcattgg ttaatcataa
     3601 taatggtggt atagtaaatc aagctacgcc aacatcagtg caatcaagta cgccttcagc
     3661 acaaaacaat aatcatacag atggcaatac aacagcaact gagacagtgt taaacgctaa
     3721 taataatgat gtagtgtcga ataataccac attaaatgta ccaaataaaa caaatgaaaa
     3781 tggttcagga ggacatctaa ctttaaagga aattcaagaa gatgttcgtc attcttcaga
     3841 taaaccagag ctagttgcaa ttgctgaacc agcatctaat agaccgaaaa agagaagtag
     3901 acgtgcggca ccggcagatc ctaatgcaac tccagcagat ccagcggctg cagcggcagg
     3961 aaacggtggt gcaccagttg caattacagc gccatatacg ccaacaactg atcctaatgc
     4021 caataatgca ggacaaaatg cacctaacga agtgctgtca tttgatgaca atggtattag
     4081 accaagtacc aaccgttctg tgccaacagt aaacgttgtt aataacttgc cgggcttcac
     4141 actaatcaat ggtggtaaag taggggtgtt tagtcatgca atggtaagaa cgagcatgtt
     4201 tgattcagga gataataaga actatcaagc acaaggaaat gtaattgcat taggtcgtat
     4261 acttggaact gatacgaatg accatggcga ttttaatggt atcgagaaag cattaacagt
     4321 aaatccgaat tctgaattaa tctttgaatt taatacaatg actactaaaa acggtcaagg
     4381 cgcaacaaat gttattatca aaaatgctga tactaatgat acgattgctg aaaagactgt
     4441 tgaaggcggt ccaactttgc gtttatttaa agtacctgat aatgtgagaa atctcaaaat
     4501 tcaatttgta cctaaaaatg acgcaataac agatgcgcgt ggcatttatc aactaaaaga
     4561 tggttacaaa tactatagct ttgttgactc tatcggactt cattctgggt cacatgttta
     4621 tgttgaaaga cgaacaatgg atccaacagc aacaaataat aaagagttta ctgtaacaac
     4681 atcattaaag aataatggta attctggtgc ttctctagat acaaatgact ttgtatatca
     4741 agttcaatta cctgaaggtg ttgaatatgt gaacaattca ttgactaaag attttccaag
     4801 taacaattca ggcgttgatg ttaatgatat gaatgttaca tatgatgcag caaatcgtgt
     4861 gataacaatt aaaagtactg gaggaggtac agcaaactct ccggcacgac ttatgcctga
     4921 taaaatactc gatttaagat ataaattacg tgtaaataat gtgccgacac caagaacagt
     4981 aacatttaac gagacattaa cgtataaaac atatacacaa gatttcatta attcacctgc
     5041 tgaaagtcat actgtaagta caaatccata tactatcgat atcatcatga ataaagatgc
     5101 attacaagcc gaagttgaca gacgcattca acaagctgat tatacatttg catcattaga
     5161 tatctttaat gatcttaaaa aacgtgcaca aacaatttta gctgaaaatc gtaacaatgt
     5221 accattaaat aaaagagtat ctcaagcaga tattgataca ttaactaatc agatgcaaca
     5281 tactttaatt cgaagtgttg atgctgaaaa tgcagtaaat caaaaagctg atcaaatgga
     5341 agatttagtt aatcaaaatg atgaactgac agatgaagaa aaacaagcag caatacaagt
     5401 tatcgaggaa cataaaggca atattattgg tgatattggt gatcaaacga ctgatgatgg
     5461 cgttactaga atcaaagatc aaggtataca gaccttaagt ggggatactg caacaccggt
     5521 tgttaaacca aatgctaaaa aagcaatacg tgataaagca acgaaacaaa gggaaattat
     5581 caatgcaaca ccagatgcta ctgaagacga gattcaagat gcaataaatc aattagctac
     5641 ggatgaaaca gatgctattg ataatgttac gaatgctact acaaatgctg acgttgaaac
     5701 agctaaaaat aatggcatca atactattgg agcagttgtt cctcaagtaa ctcataaaaa
     5761 agctgcaaga gatgcaatta accaagcaac agcaacgaaa agacaacaaa taaatagtaa
     5821 tagagaagca actcaggaag agaaaaatgc agcattgaac gaattaactc aagcaaccaa
     5881 ccatgcttta gaacaaatca atcaagcaac aacaaatgct gatgttgata acgccaaagg
     5941 agatggtcta aatgccatta atccaattgc tcctgtaact gttgttaagc aagctgcaag
     6001 ggatgctgta tcacatgatg cacaacaaca tatcgcagag atcaatgcta atcctgatgc
     6061 gactcaagaa gaaagacaag cagcaattga caaagtgaat gctgctgtaa ctgcagcaaa
     6121 cacaaacatt ttaaacgcta ataccaatgc tgaagtggaa caagtgaaga caaatgcgat
     6181 acaaggaata caagcaatta caccagctac aaaagtaaaa acagatgcaa aaaatgccat
     6241 tgataaaagt gcggaaaagc aacataatac gatatataat aataatgatg cgacgctcga
     6301 agaacaacaa gcagcacaac aattacttga tcaagctgta gccacagcga agcaaaatat
     6361 taatgcagca gatacgaatc aagaagttgc acaagcaaaa gatcagggca cacaaaatat
     6421 agttgtgatt caaccggcaa cacaagttaa aacggatgct cgcaatgttg taaatgataa
     6481 agcgcgagag gcgataacaa atatcaatgc tacacctggc gcgactcgag aagagaaaca
     6541 agaagcgata aatcgtgtca atacacttaa aaatagagca ttaactgata ttggtgtgac
     6601 gtctactact gcgatggtca atagtattag agacgatgca gtcaatcaaa tcggcgcagt
     6661 tcaaccgcat gtaacgaaga aacaaactgc tacaggtgta ttaaatgatt tagcaactgc
     6721 taaaaagcaa gaaattaatc aaaacacaaa tgcaacaact gaagaaaagc aagtggcttt
     6781 aaatcaagtg gatcaagagt tagcaacggc aattaataat ataaatcaag ctgatacaaa
     6841 tgcggaagta gatcaagcgc aacaattagg tacaaaagca attaatgcga ttcagccaaa
     6901 tattgttaaa aaaactgcag cattagcaca aatcaatcag cattataaag ctaaattagc
     6961 tgaaatcaat gttacaccag atgcaacgaa tgatgagaaa aatgctgcga tcaatacttt
     7021 aaatcaagac agacaacaag ctattgaaag tattaaacaa gctaacacaa atgcagaagt
     7081 agaccaagct gcgacagtag cagagaataa tatcgatgct gttcaagttg atgtagtaaa
     7141 aaaacaagca gcgcgagata aaatcactgc tgaagtggcg aagcgtattg aagcggttaa
     7201 acaaacacct aatgcaactg acgaagaaaa gcaggctgct gttaatcaaa tcaatcaact
     7261 taaagatcaa gcaattaatc aaattaatca aaaccaaaca aatgatcagg tagacacaac
     7321 tacaaatcaa gcggtaaatg ctatagataa tgttgaagct gaagtagtaa ttaaaccaaa
     7381 ggcaattgca gatattgaaa aagctgttaa agaaaagcaa cagcaaattg ataatagtct
     7441 tgattcaaca gataatgaga aagaagttgc ttcacaagca ttagctaaag aaaaagaaaa
     7501 agcacttgca gctattgacc aagctcaaac gaatagtcag gtgaatcaag cagtaacaaa
     7561 tggtgtatca gcgattaaaa ttattcaacc tgaaacaaaa gttaaaccag ctgcacgtga
     7621 aaaaatcaat caaaaagcga atgaattacg tgctaagatt aatcaggata aagaagcaac
     7681 agcagaagaa agacaagtag cactagataa aatcaatgaa tttgtaaatc aagccatgac
     7741 agatattacg aataatagaa caaatcaaca agttgatgat acaacaagtc aagcgcttga
     7801 tagcattgct ttagtagcgc ctgaacatat tgttagagca gctgctagag atgcagtaaa
     7861 gcaacaatat gaggctaaaa agcaagaaat agagcaagct gaacatgcga ctgatgaaga
     7921 aaaacaagtt gctttaaatc aattagcgaa taatgaaaaa cgtgcattac aaaacatcga
     7981 tcaagcaata gcgaataatg atgtgaaacg tgttgaatca aatggtattg ctacgttaaa
     8041 aggcgtagaa ccgcacattg tggttaagcc tgaagcacaa caagcaataa aagcaagtgc
     8101 agaaaatcaa gtagaatcaa taaaagatac accacatgca acagttgatg aattagatga
     8161 agcgaatcaa ttaattagcg acacactcaa acaagcgcaa caagaaatag aaaatacaaa
     8221 tcaagatgct gctgttactg atgttagaaa tcaaacaatc aaggcaatag agcaaataaa
     8281 acctaaagta agacgtaaac gagctgcgct tgatagcatt gaagaaaata ataaaaatca
     8341 actcgatgca atccgaaata cgttggatac tactcaagat gaaagagatg ttgctattga
     8401 tactttaaat aaaattgtaa atacaattaa aaatgacatt gcacaaaaca aaacgaatgc
     8461 agaagtggat cgaactgaga ctgatggcaa cgacaacatc aaagtgattt tacctaaagt
     8521 tcaagttaaa ccagcagcgc gtcaatctgt tggtgtaaaa gccgaagctc aaaatgcact
     8581 aatcgatcaa agcgatttat caactgaaga agaaagacta gctgctaaac atttagtaga
     8641 acaagcactt aatcaggcta ttgatcagat caatcatgca gataagactg cccaagttaa
     8701 tcaagatagt ataaatgctc aaaatattat ttcaaaaatt aaaccagcga caacagttaa
     8761 agcaacagca ttacaacaaa ttcaaaatat cgctacaaat aaaattaatt taattaaagc
     8821 aaataacgaa gcgacagatg aagaacaaaa tattgcaata gcacaagttg aaaaagagtt
     8881 aattaaagct aaacaacaaa ttgctagtgc agtgactaat gctgatgtgg catatttatt
     8941 gcatgatgag aaaaacgaaa ttcgtgaaat cgaacctgtt attaacagaa aggcgtctgc
     9001 tcgagaacaa ttaacaacat tgctcaatga taaaaaacaa gcaattgaag cgaatgttca
     9061 agcaacggta gaagaaagaa atagcgtttt agcagaatta caaaatattt atgacgcagc
     9121 aatcggacaa cttgatcatg accgtagcaa tgcacaagtg gataaaacag catcttttaa
     9181 tcttcaaaca atacatgatt tagatgtgca tcctgttaaa aaaccagatg ctgaaaaaac
     9241 gattaacgtt gatcttgtac gcgtcactgc tttagtgcaa aattatcgaa aagtaagtga
     9301 tcgtaataaa gttgatgcat taaaagctat aactgcatta aaattacaaa tggatgaaga
     9361 attaaaaaca gcacgcacta atgctgatgt tgatgcagtt ttaaaacgat ttaatgttgc
     9421 attaagcgat atagaagcag taattactga aaaagaaaat agcttactgc gcattgataa
     9481 cattgctcaa caaacatatg cgaaattcaa agcgatcgca acaccagaac aattagctaa
     9541 aataaaagta ttaattgatc aatatgttgc agatggcaat agaatgattg atgaagatgc
     9601 gacattaaat gacatcaaac aacatacgca attcattgtt gatgaaattt tagcaattaa
     9661 attaccagct gaagcgacga aagtatcacc aaaagtaatt cagccagctc caaaagtttg
     9721 tacgcctatt aaaaaagaag agacacatga atcgcgcaaa gttgaaaaag aacttccaaa
     9781 tacaggttct gaaggaatgg atttaccatt gaaagaattt gcactgatta caggtgcggc
     9841 tttgttagct agaagacgta ctaaaaacga aaaagaatca taattaacga aatgacctta
     9901 atataacgac acttttaagt gactcagtta tattaaggtc atttttaatt gaatgaaaaa
     9961 gttgatctac taaagagaat cgaaccaaat tttatactat tattccattg atgtatgtga
    10021 gtaatgaccg gcatataatc cagcgacatg tcctgttacg agtgcacttg taatattata
    10081 accaccagta taaccatgta tatctaatac ttcaccacat aaaaataatc ccggaactaa
    10141 tttagacatc attgttttag gttgaatttc tttaagtgac acaccaccac ctgtgacaaa
    10201 tgccttatct ataggtaatg tcccgttcac cttaaataca aaccctttaa acatatttac
    10261 taagtcgttc aattgttgat ttgataagtg atgtgatgtg gtattttcat cgattccagc
    10321 ttgttccagc atgaacagta agtagcgctc ttcaattaga ccatgcaaac tgtttttaat
    10381 gattttatct ggtgtgtccg ataataatga tgtgatgtgt tgttttaatt gttcatggtt
    10441 taattcagga aatgcatcga ttgccataga aatgtgctgt gtcttttgat ttttttgttc
    10501 tttataaaca aactgactac atcttaatgc agctggacca ctgataccaa aatgagtaaa
    10561 taacatatcc atttgatgac tgatgcgttt tttaccattt ttcttaagta ctgacaattc
    10621 aacatctttt aaacttaaac cttttagacg attggatttg atgaaaggtt cagctgatgt
    10681 aattggaact tcggtcggga ataactcagt aatggtatga cctaaatctt gtgcgaactt
    10741 ataaccatca ccagttgaac cagtttgagg gacacttgtt ccacctgtag caatcactag
    10801 cgaatggctt tcataactat tattttgagt atgtacagtg aaagtttggt ctgtattaac
    10861 ttcgattcta ctaacagctt cttcttcttt aatcgttaca tgttggcgtt cgatagttgt
    10921 cactaatgta tcaaccacgt cttgtgcttt gttggaaact ggaaacatac gtccgtgatc
    10981 ttcttctttt aatttaacac ccctagactc aaaaaaatct atgatggatt cattatcaaa
    11041 aattgaaaag ggactatata aaaatttccc atttccagga atgttcttaa taatttcagc
    11101 atatggtaat cgattagtta cgttacatct accgccacca gatattttga gtttacgacc
    11161 tagacctttc tttttttcaa tgagtaacac actgctactt tgttcgcttg cagctactgc
    11221 cgccattaag ccgctaggtc cgcctccgat aataattgtt tgatacatat acgcttcctc
    11281 caaatttgtt actggacatt caattgctat tttatagtac ttttttaaac tttgctatat
    11341 ctcttgtgtt atgagatgac aattatgttg aatatattta taatgtgaac ttcataaaaa
    11401 atgaagatgt atgtgtatga taagaatgat ttttctactg ttatgcgatg ttcatctcaa
    11461 atagtagtat aatgtttata tgaaatttcg taatattcta atcaaaatcg agtattcaat
    11521 gacaatgcgt tgataatttt gttatgataa ttcattgaat atacgacaat gaaataaagt
    11581 tttgtgatat attcataata atgaaaaatg attttacaga gaaatagctt tgagtttaca
    11641 aaactttaat aaacatgaga tagggagatt cgtaatgagt gaaagtaaag aaatggtgcg
    11701 tggaaccttt ttaattacca ttagtatatt aattaccaag gtattgggcg tactttttat
    11761 cattccattc aactatttaa ttggtggaca agaaaatatg gcgccgttca catacgctta
    11821 tgcaccatat aatattgcaa ttgcagttgc gacagcaggt gtaccattag cagcttcgaa
    11881 atatgttgcg aaatataatg ctattggggc atataaagtc agtcagaaat tttataaatc
    11941 gagttttatt gtaatgagta ttaccggtgt tttaggtttc ttggtactgt atttcctagc
    12001 accttatatt tctgaattaa cattagctag aaacgttcat gataaaaacg gttggtcagt
    12061 cgatgatatt acttggatta taagaatcat tagtatggtt gttatcttta ttcctgtatt
    12121 agcgacatgg agaggtatat tccaaggtta taaatctatg gggccaacag cagtgtcaga
    12181 agtaactgag caaattgcac gcgtgatttt catattaatc ggaagttatt tagtactaaa
    12241 tgtctttgat ggctctattt tattagccaa tggtattgct acttttgcag cagcagttgg
    12301 tgcgattatt ggtatattca cactctggta ttactggaga aagcgtaagc ataatattga
    12361 tcgtatggtc gaatctgatt acacagatat agatgtgtca tatggcaaga tgtataaaga
    12421 aattattgct tatagtattc cgttcgttat tgtaagtttg aattatccat tatttaactt
    12481 agtagatcaa tttacacata acggtgcttt atctttagtt ggtatacctt cacagttaca
    12541 agatatattc tttaatatgc taaatatgtc aactaataaa attgtaatga ttccgacatc
    12601 attaagtgct ggttttgcag tgagtttaat tccttttatt acaaaaacat ttgcagaagg
    12661 tcgcttacat gaaatgcatc atcaaattag aacatctatt ggtgtattaa tgtttataac
    12721 tgttccagct agtattggaa ttatggcctt ggcacaacca ttattcacag ttttctatgg
    12781 ttacgatcca attgttttag gtcatgaccc taaccatgat ggtagtcgct tattattcta
    12841 ttatgcgcct gtagcaatac taatttcact attaagtgta acggcatcaa tgttacaagg
    12901 tatagataaa cagaaattaa cagtttatgt tattttagct tctgtagtta tcaagttagc
    12961 gttaaactat ccattaatta tgttattcca tacacctggt gcaattttaa gtactagtat
    13021 cgcattatta tttgcaattg gatgtaattt ctatattctt aaaaaatatg cgaagtttaa
    13081 attctgctat agttggattc attttgcaaa aatattcttg tattcattta ttatgatgct
    13141 aggtgtagaa ttagtatttt tccttgcaaa tctattctta gaacctacta aacttggtta
    13201 cttaatcatt attattttag gtgttacagt aggtattcta atttacggta caatcacaat
    13261 taaaacgcgt cttgcggacg aattcttagg tgaaattcct gaaaaattaa gacgtagagt
    13321 taggttttta cgatgagaat agataaattt ttagcaaata tgggcgtcgg tacacgtaat
    13381 gaggtcaaac agttacttaa aaaaggcctt gtaaatgtca acgaacaagt aattaaatca
    13441 ccaaaaacac atatagaacc agaaaacgat aagataacag ttcgtggaga attaatagaa
    13501 tacgttgaaa atgtctatat tatgttaaat aagccaaaag gttatatatc cgcaactgaa
    13561 gatcatcatt caaaaaccgt tattgattta atacctgaat accaacatct aaatattttt
    13621 ccggttggtc gtcttgataa agatacagaa gggcttttat tgataacgaa tgatggtgat
    13681 tttaatcatg aattaatgag tccaaacaaa catgtttcta aaaaatatga agttatttca
    13741 gcaaatccta tcactgaaga cgacattcaa gcttttaaag agggtgtaac actgtctgat
    13801 ggcaaggtta agcctgcaat attaacttat attgataatc aaacgtcaca cgtaacaatt
    13861 tatgaaggaa aatatcatca agttaaacga atgtttcata gcattcaaaa tgaggtatta
    13921 catttaagac gtatcaaaat tgctgattta gaattggaca gcaatttaga ttcaggagaa
    13981 tatcgtttat taactgaaaa tgattttgac aaattaaatt ataaataaaa gggagacgat
    14041 tgatatggca aaaggtaatt tatttaaagc gattttaggt ataggtggcg ctgtagcagc
    14101 tgtacttgtt acacgtaaag atagtcgtga caagctgaga gcagaatata ataaatataa
    14161 acaagatcct caaagctata aagataatgc taaggataaa gcgacgcaat taggaacaat
    14221 tgctaatgaa acaattaaag aagtaaaaac aaatccgaaa gaatatgcta atagattaaa
    14281 aaataatcca aaagcatttt tcgaagaaga aaaatcaaaa tttaccgaat atgacaataa
    14341 gactgacgaa agtattgaaa aaggtaaatt tgatgatgaa ggtggcgcag caccaaataa
    14401 taatttacgt atcgtcactg aagaagattt aaaaaagaat aaaaatgcat tatctgataa
    14461 agaataagat gtgagtagga cagaaatgat aaagaaccac tatgatttat tatatagtgg
    14521 ttctttatcg ttagtcatag ctaatatgca tatagaaaga agaatacatg cgtgaaactc
    14581 attcataaga aatactaata tctaaagaaa acgtatttct ttatcttttc gaaatt
//
LOCUS       DAHHYJ010000063        14584 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000063 DAHHYJ010000000
VERSION     DAHHYJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14584
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.063"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..365
                     /locus_tag="NBK22_001434"
     CDS             <1..365
                     /locus_tag="NBK22_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCD2414625.1"
                     /translation="SNENMTFYHHGVYLLLNEDFKKLPVQLYLTEFIYHLLIQISLKY
                     PEVYFLNLLSQFDDLKNNIQEISTKHEILHNHTRRSKIAEFIDSKEYQKLMNKIDVMK
                     EKLLNKNILKKQGDTHDT"
     gene            355..1461
                     /locus_tag="NBK22_001435"
     CDS             355..1461
                     /locus_tag="NBK22_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2414626.1"
                     /translation="MTHNLNLSHNIYKDTKRGTYYFRITYYDKSNTRKYITRKGFKQR
                     KDAVKKCNEMMDELEGVGHLNRLPFDKLVEEYIDWYSARRKTSSVKALKTHTNNHLLP
                     YFKSMDVFKMTTQDVMKFQNKKLKEGHSGEYLKKMHVYLVSLLNHAMKFHELKQNVAS
                     LVGNFEIESQKRLNYWTLEQFNQFYDALATQQQKLFFKLLFYSGARKGEIRALTWRDV
                     NFEDEFIHINKTDYHGEVTAPKTKSAIRDIYLPTHMMYDIKDYLIWYKENNIYKDDYV
                     LFGTFYKAYSESTIDRWFTNALKVLDEQLPNGQNFPRIVIHELRHSHASMLVNLGASV
                     MIIAQRLGHSDTTEVYNRYGHLYPSTQKEIVKYL"
     gene            complement(1572..1933)
                     /gene="ssrA"
                     /locus_tag="NBK22_001436"
     tmRNA           complement(1572..1933)
                     /gene="ssrA"
                     /locus_tag="NBK22_001436"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2024..2488)
                     /gene="smpB"
                     /locus_tag="NBK22_001437"
     CDS             complement(2024..2488)
                     /gene="smpB"
                     /locus_tag="NBK22_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCD2414627.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            complement(2510..4882)
                     /gene="rnr"
                     /locus_tag="NBK22_001438"
     CDS             complement(2510..4882)
                     /gene="rnr"
                     /locus_tag="NBK22_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCD2414628.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSRGQSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAIDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEDSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPHVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPNIREQY
                     KEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTVKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGRKKKGKQRKGKNQRNNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            complement(4916..5656)
                     /locus_tag="NBK22_001439"
     CDS             complement(4916..5656)
                     /locus_tag="NBK22_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCD2414629.1"
                     /translation="MQIKLPKSFFFGEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILKSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGTIYEGFLEYARNFKKYEGKDQETIDNEMDHFKPTET
                     LKELSEALDTIKEQVDEVLDPILVIQAENDNMIDPQSANYIYDHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            complement(5772..6005)
                     /gene="secG"
                     /locus_tag="NBK22_001440"
     CDS             complement(5772..6005)
                     /gene="secG"
                     /locus_tag="NBK22_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCD2414630.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            complement(6072..6530)
                     /locus_tag="NBK22_001441"
     CDS             complement(6072..6530)
                     /locus_tag="NBK22_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414631.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            complement(6869..8173)
                     /gene="eno"
                     /locus_tag="NBK22_001442"
     CDS             complement(6869..8173)
                     /gene="eno"
                     /locus_tag="NBK22_001442"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCD2414632.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            complement(8303..9820)
                     /locus_tag="NBK22_001443"
     CDS             complement(8303..9820)
                     /locus_tag="NBK22_001443"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCD2414633.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            complement(9823..10584)
                     /locus_tag="NBK22_001444"
     CDS             complement(9823..10584)
                     /locus_tag="NBK22_001444"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCD2414634.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGTQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDEEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSEDQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            complement(10706..11896)
                     /locus_tag="NBK22_001445"
     CDS             complement(10706..11896)
                     /locus_tag="NBK22_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCD2414635.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     INNK"
     gene            complement(12035..13045)
                     /gene="gap"
                     /locus_tag="NBK22_001446"
     CDS             complement(12035..13045)
                     /gene="gap"
                     /locus_tag="NBK22_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD2414636.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            complement(13098..14111)
                     /locus_tag="NBK22_001447"
     CDS             complement(13098..14111)
                     /locus_tag="NBK22_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCD2414637.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
BASE COUNT         4494 a         2872 c         2212 g         5006 t
ORIGIN      
        1 tctcaaatga gaacatgaca ttttaccatc acggtgttta tttattgcta aacgaagatt
       61 tcaaaaaatt acctgtacaa ttatatctca ctgaatttat ttatcattta ttaatccaaa
      121 tttcattaaa atatcctgaa gtctactttt taaacttgtt gtcgcaattt gacgacctca
      181 aaaataatat acaagaaatt tcaactaagc atgaaatatt acacaatcat actagaagaa
      241 gtaaaatagc agaatttata gactctaaag aataccaaaa attaatgaat aaaatcgatg
      301 ttatgaaaga aaagttactc aataaaaata ttttaaaaaa acaaggcgat actcatgaca
      361 cataacttaa atctatccca taatatatat aaagacacta aacgcggtac ttattatttc
      421 cgtatcactt actatgacaa gagcaatact cgtaagtaca taacacgtaa ggggtttaaa
      481 caacgtaaag atgcagtaaa gaaatgtaac gaaatgatgg acgaattaga gggagtcgga
      541 caccttaata gattaccttt tgacaagctc gttgaagaat atatagactg gtattcagca
      601 cgtcgaaaga catcaagtgt aaaagcatta aagacacata ccaataacca tttgctacct
      661 tattttaaat ctatggatgt atttaaaatg actacacaag atgtgatgaa atttcagaat
      721 aagaagttaa aagaggggca ttctggagaa tatttaaaga agatgcatgt atatttagta
      781 tcattactga atcatgcaat gaagtttcat gagttaaaac aaaatgttgc atctcttgta
      841 gggaattttg aaatagaatc acagaaacga ttgaattatt ggacgttaga acaattcaat
      901 caattctatg atgcgcttgc tacacaacaa caaaaattat ttttcaaact attgttctac
      961 tctggagcaa gaaagggcga aattagagct ctcacatggc gtgacgttaa ttttgaagat
     1021 gaatttatcc atataaacaa aacggactat cacggtgaag tgacagcccc taaaacgaaa
     1081 tcagccatac gtgatatata tttgcctact cacatgatgt atgacatcaa agattattta
     1141 atttggtata aagagaataa catatacaag gacgactatg tattgtttgg tacattctat
     1201 aaagcttaca gcgagtctac tattgatcgt tggtttacta acgcattaaa agtgttggat
     1261 gagcaattac caaacggaca aaatttccct agaatcgtta tacacgagtt aagacatagc
     1321 catgcatcta tgctagttaa tctaggggct agtgtaatga ttatagctca gcgtttaggt
     1381 cacagcgata cgactgaagt atataaccga tatggtcatt tatatcctag tacacagaaa
     1441 gaaatagtta aatacttata aggcactacc aactaaaatt ggtggtgtct ttttatatcc
     1501 tgtgcatttt ctgtgcacta tctgtgcact caaaaataaa aagcctacaa ccactaaggt
     1561 cgcagactat ataatggaga cggcgggatt tgaacccgcg tccagaggtc ctgatacaca
     1621 tctttctacg tgtgtagttt atcgaaaggt ttcgcatcat gaaaagtgat aaacaaccaa
     1681 catgatgcta gcttgattaa gtttcttcta aacagacttc aaacggcagt gtttagcata
     1741 tcctattaag gttgaatcgc gttaacagca cataggaaat gctgttaggc gatgcagagt
     1801 gcgattaggc agctactgcg aaattattgt ttgatttgcc agttattata aactgtgtgt
     1861 gttgatgacg aagacaaccc tccgacacgc ttaatgagct cgggggaccc ctgtcgaatc
     1921 catgaacgtc cccgaaataa taaatcaaac aaatcatact tttgtaattt acaaaagcac
     1981 atataactat atcaaatata gcccgattaa gcaactaaat cgcttaataa cgggctttca
     2041 tatcgcgcgc aacatctcgt ttgactgctt tttctttcaa agcttgacgt ttatcatatt
     2101 ttttcttacc tcgtgcaaca ccaagtaata ctttacaatg tccatgcttc aaataaagct
     2161 ttaacggcac aatcgaataa ccaatctcac gtgtttgatc acccaattta atgatttcac
     2221 gcttgtgcaa taataatttt cgagaacgaa gaggatcgtg attaaaacga ttcccttctt
     2281 cgtatggtgc tatatgcata ttattcaaat acatttcacc gtttttaact tgcgcataac
     2341 tatctttaag gttagcacta cctcggcgaa tcgattttat ttctgtacct tgtaatacga
     2401 ttcccgcttc aatcgtgtct tcaatattat aatcatgtct tgctttacga ttttccgcta
     2461 atgtacctgg tgatttcttc ttagccattc atactcacct cattgctgct tattttttct
     2521 tacgacgtgc tttctttttc acacttttat ctttataaaa tggcttatgc ttactattac
     2581 ctgatttatc attattacgt tggtttttac ctttacgttg cttacctttt ttcttacgac
     2641 ccttatcatc agatttcgat ttatctaaag atttgccacg cgttttagct tgaatcgttt
     2701 tacctcttgc tggtcgctgt gagcggtcat ttttaggtaa aggcattcca acaatttgaa
     2761 aatcaattaa tcgttcatct acatcaacat gcgtcacttt aaccttaact gtgtcaccaa
     2821 ttctaaatac tttagcttga cgctcaccaa ttaatgccat ttgacgctct tcaaaacggt
     2881 aataatcatc agtcatattc gcaatatgaa ccataccttc tatcgtattt ggcaattcaa
     2941 tgaacatacc gaagttagct actgagctga caataccttc aaattcatca ccaatatgtt
     3001 gaatcatata ttctgctttt ttcaattcat cagtatcacg ttctgcctca atagcacgac
     3061 gttcacgttt agaagtatgt tcagctaact caggcaattt gtcttcccaa cgcttcactt
     3121 ctttgttatc cattgatttc tcaattaaat acttacggat taaacgatga actgttaaat
     3181 caggataacg tctaattggt gatgtaaaat gcgtataata ttcagctgat aagccaaaat
     3241 gtcccaagtt cacatcatca taatgcgctt gttgcattga acgcaacatc attgttgaaa
     3301 tgaccatttg ttcaggtcga ccttctactt cttcttgaac cttttgaagt gttgttggat
     3361 gaatatcttc gccagtaccc ttaatcatga tgccaaagtt tgtaataaaa tcaaagaatt
     3421 gtcttaagcg atctgattta ggttgctcat gcactcggta aataaaaggt acatctaact
     3481 tactaaaatg ttcagcaact gtttcatttg caattaacat aaatgattca attagacgtt
     3541 caccctcgcc acgttgtctt aattgaacat ctgttggtat accgtcttcg ttaactaata
     3601 cttttgcttc actaatatca aaatcgattt caccacgtcg ttttctcatt tgaatcaaac
     3661 gattagataa atcttgtgct aaatctaaca taggcgtaat ttctttatat tgttcgcgaa
     3721 tgttaggatc cttttcagta ataatctgat ttaccgcatc atacgtcatt cgataatcag
     3781 aatgtataac actatcaaaa atttcatgtt taacaacgcg accactagcg tcaatttcca
     3841 tgcgacagct taacgttaaa cgatcaacgt gaggatttaa tgaacaaata ccattgctta
     3901 atcggtgtgg aatcatcgga atcacacggt caacaagata tacacttgtc gctctatcat
     3961 aagcttcttt atctaaagct gaatcttcag ttacataata actgacatca gcaatactta
     4021 cagtcaactg cgtattaccg ttcgctaact ttttaacact aattgcgtca tctaagtctt
     4081 tagcatccgc accatcaatt gtgattgtta attcatcacg taaatcatga cggcctttaa
     4141 tttcagtatt ttcaatatga tcaggtactg cttcagcttc ttgtaacact tcatcaggaa
     4201 attcaatttc tatgccatgt tgatagataa tagataaaat atctacgcca ggatcatttt
     4261 tatgtcctaa tattgctgaa atatgccctt caggattatc tgaaccatca gcatatttag
     4321 taatttgaac aagtacctta tgcccatcta ttgcacctaa actttgtcct ttaggaatga
     4381 aaatatcttg cataattcgt ttatcatctg gaataacaaa accaaaatgt ctagcttcac
     4441 tatacgtacc aaccacttga gttactgaat gcttctcaat tgacttaact tccccttcaa
     4501 ttttaccttt atgctcacct tttgattgat ggatttctac aataacagta tctccatcca
     4561 aggcacgatt aattttcgtc gggggaataa atatatcttc catatcctca tcttcaggtc
     4621 ttaagaatgc aaagcctttt ttattttgac ttaacgttcc ttttatcaat tttgattgac
     4681 ctctagaact atgctttttt tggtatctgt ctgtttttgt acgttcgatt aatcctgatt
     4741 gttctaactc cacaagcacc ttaattaaat ctctaaacga gtcggcactg cttaaaccta
     4801 atgcatcttg aaaatctgac actgacatag gttcatattc aggttgatta ataatctctt
     4861 ctattgattg ctttaaattc attatgcccc tcctttctaa tgttaaaatc tctttttatt
     4921 ctgaccagtc taatgactct aaaaattgat aaatatcttc aaatacttgt tctttctctt
     4981 tatcaatcgt aataacatgt ccagattcac tgtaccactt gatatttttg tcatcagaat
     5041 ctacatggtc atatatataa tttgcggatt gtggatcaat catattgtcg ttttctgctt
     5101 gaatcactaa aataggatcc aacacttcat caacttgctc tttaatcgta tctaatgctt
     5161 cacttagttc ttttaaagtt tcagttggtt taaaatgatc catttcatta tcaatagtct
     5221 cttgatcttt accttcatac tttttaaagt tacgtgcata ctctaaaaag ccttcgtaaa
     5281 tggtaccttc agttttgcca cccattggtg cacacatcgt tacaataccc tttacatctc
     5341 tatttaagct taattttaaa gcaaaatccc cacctaatga tagaccagca acaacaattt
     5401 catcataacc ttgttcaaca agataatcat aaccatctaa cgcatcttta aaccaaacga
     5461 aaggactaga tttcagtatt tcctctggtg gtgccgcgtg accttcatat tgtggtgcat
     5521 acgaagtata tccctttttt tgtaaaaatc gacctaattg acgtacatca gacgaattgc
     5581 ctgtaaatcc atgtaataat aacacggcac gtttgccttc tccaaaaaag aaagattttg
     5641 gtaattttat ctgcataaat ctaacgctcc ctttgcatac ttaactaggc tatatatttt
     5701 acatagctta ttgttaaact attacattct taattataaa taaaaaagcc cgacatttac
     5761 atcgccggac cttacatacc aagataactt atgcaaatca taagtacaaa aaataatatt
     5821 gataaaataa ttgttaatct atttaagaat aaatcgacgc cacgttgttt ttgtttaccg
     5881 aataactgct cagcaccacc actgatggca cctgaaagtc cactgctttt accttcttgg
     5941 agtagtacaa cagttattaa tgcaatacaa tcaatgatta ataatacgat taaaaatgta
     6001 tgcataaatt gtcctccgtt ccttatatac gacaagtagt atatcatatt tttattgtta
     6061 aaatcaattt tctagcgatt aaatgtactt ttacgtttga tagaaacaac aagcatataa
     6121 atcgctaatg caaccgaacc tataataaca attaaaattg gcaatgttaa gtacgcaccg
     6181 tgtgcagctt ttattgcttg aattgtgatt gataaattaa tcaacgtaaa caaactattt
     6241 gatacataaa ctgcaaacat aaaccaccac acataagtat gtcgattcca aaaagtgatg
     6301 aagcctgcta gataagccaa aatatacata ataccagtga atctaaaact gtttaaaatg
     6361 taatctatcg ctttatctga agtgttttga ttcgttaaat taagtaatgc tttggcagtc
     6421 gcattatcca acgaaacaaa gtaatgaata atcaacaata ttgagaatac gattgatgca
     6481 gttgcaataa agcgtcctgt tttaatttct ttattcgttc tatcagccat ttgtttactc
     6541 ctcatatatt taaatacgtt ctataaaacc tttatttaat tttacctttt aataaaagtg
     6601 aataccctgt catacaatat tcatttcaac ataaatttat tataccagtt taagaataca
     6661 agttaaatat gtgttattag ttacctcatg attattagtc tgttatgaat aatacttcaa
     6721 aataaaaaac ctaccacagt ctcaactctg tggcaggtta taaatacaag cgtgaacttt
     6781 ttaagcgtga actcaaaaac tttattcaat ttataccgac ataataatcc tcaactaaaa
     6841 ttatggcagt acttaatgtt taaaatgatt atttatctaa gttgtagaat gatttgatac
     6901 catcatattt agcagtttca aataattcat cttcgatacg taataattga ttgtatttag
     6961 caatacggtc agtacgtgat aatgaaccag ttttaatttg accagcgttt gtagcaacag
     7021 cgatatcagc aattgttgta tcttctgttt cacctgaacg gtgagaaact actgctgtgt
     7081 aaccagcttt ttgagccatt tcgattgcat caaatgtttc agttaatgta ccgatttggt
     7141 taactttaat taagattgag ttaccaattc cgttttcaat accttttgct aaaatttcag
     7201 tgtttgttac gaataaatcg tcacctacta attgtacacg gtcaccgata cgttctgtaa
     7261 gttgtttcca accatcccag tcgttttcgt ccataccgtc ttcaattgta atgataggat
     7321 atttgtctac taattgttct aagtagtcaa cttgttctgc agctgtacgt tttgcaccgt
     7381 gttcgccttc gaacttactg tagtcatata caccattttc atagaattct gatgatgcac
     7441 agtcaaatcc taagaatact tcttcacctg gtttgtaacc agctgcttcg attgcttgga
     7501 taattgtttc aacagcatct tcagtacctt caaatttagg agcgaaacca ccttcgtcac
     7561 ctactgcagt ttctaaacca cgtttgctta aaattgattt taagttgtgg aaaatttcag
     7621 taccccaacg taatgattct ttgaacgttg tagcacctac aggtaaaatc atgaattctt
     7681 ggaatgcaat tggagcatct gagtgagaac caccattaac gatgttcatc attggtactg
     7741 gtaactgctt accattaaat ccacctaaat atttgtaaag tggttgacct aataagtcag
     7801 ctgctgcacg tgctactgcg atagatacac ctaaaatagc atttgcacct aatttacctt
     7861 tgtttggagt accgtctaat gcgatcatca ttttatcaat agatacttga tctaatactg
     7921 aaaattcacc ttcaataatt tctggtgcga tgatttcatt aacgttttca actgctttag
     7981 taacaccttt acctaaataa cgtgatttgt ctccatcacg taattcaaca gcttcgtgtt
     8041 caccagttga agcacctgat ggtactaatg cacgaccaaa tgcgccactt tcagttaata
     8101 cttctacttc aacagttggg ttaccacgag agtctaagac ttcgcgagcg taaacatctg
     8161 taataattgg catgtttata atctccttta aatagttatt ctataattat tatagcttta
     8221 ttctttcatt taacaaaatg atattactat tattttcaaa aaataagtgt cattaagcgt
     8281 ttacttaaca tcttttacaa tattagtgtt taattaaaga ttcacctgtc atgtcttcag
     8341 gttgttctac atttaataaa tctaataatg taggtgctaa gtcacctaag cgaccagttt
     8401 ctcgaagtgt aacgccttct tttgttacaa tcactggtac tgggttcgtt gtatgcgtag
     8461 tcattggttg atcatcatcc gtcaatactt gatcagagtt accatggtca gcagtaataa
     8521 ttgcataacc gtccatgtct aaaatcttat caacgacttc acctaaacat tcatcaaccg
     8581 cttcgattgc tttgattgtc ggctcaagca taccactatg tccaaccata tcagggttag
     8641 caaagtttaa aataattaag tccaagtcac ctttatttaa ctcttctaat aatgcatctt
     8701 taacttcata agcactcatt tctggtttca agtcatacgt tgcaacttta ggtgaatcaa
     8761 ttaaacgacg gcgttcacct ttaaattcct cgttacgtcc accactcata aagtaagtaa
     8821 cgtgaggata tttttcagtt tctgcaatac gtaattgtgt taaattgtta ttttgtgcaa
     8881 tttcaccaat tgtattattt aaatcaactt tttcgaaaac gatagccgca tcgatattgt
     8941 cgttatactt agtgaatgtt gcatagaata agtctttaac ttgttcaact ttaaagcctt
     9001 cgaatgctct gttcgcaaaa atttccgata attgtgctgc tctatcaggt cggaaattat
     9061 agaagatcac tgcatctcca tcattaacac cgtcattttg attctcaacg atgaatggta
     9121 ctacgaattc gtcagttaag ccctcattat agcttgcttc tacaccttct ttggcagttg
     9181 cataagttgg ggcatcaaaa ttacgaatag cattgtaagc tttttcttca cgttcccaac
     9241 gtttgtcacg gtccattgca taataacgac cagacacaga tgcaaattga ccaatgccta
     9301 attcattgaa tttagcttca gtctcttcga tgtatttcaa agcggatttt tgatctacgt
     9361 cacgaccatc taaaaatgcg tgtacgtaaa ctttttcaac accttgtttt ttagcaagtt
     9421 ctaacaaagc aaataaatgt ttgtaatgac tgtgcacacc accgtcagac aataaaccaa
     9481 agatgtgtaa cgctgaatca tgtgaattca cgtgtgcaat tgcattattt aaaacatcat
     9541 tttcaaagaa atcaccgtct tcaattgatt tattgattcg agttaaactt tgataaacga
     9601 tacgtcctgc accgatattc atatgaccaa cttctgagtt acccatttgt ccttcaggta
     9661 gtccaacatc taaaccactc gcttcgattt gagtcgttgg atatttgttg taataacgat
     9721 caaaattagg cttgtttgct aattttaccg cattaccatg ttcgctttcg cggttcgcaa
     9781 aaccatctaa aataattaac gcagttggtt tcttagccat gattattttg caccttctaa
     9841 caattgtacg aaatcttcaa ctttaagtga tgcgccacct actaatgccc catcaatatc
     9901 agtttgtgcc atgtattctt taatgttgtt aggtttaaca ctaccaccat attgaatacg
     9961 agttgcttct gatacttctt tgcttgataa gtcagcaata gtttgacgta caaatgcaca
    10021 catttcattt gcatcttcag atgttgatga tttaccagtt ccgattgccc agattggctc
    10081 ataagcaatt acaactgatt taagttgatc ttcagataaa cctgcaacag ctttcttaac
    10141 ttgctcacct acaacatcgt tagctttacc actttcacgc tcttcgtctg tttcaccaac
    10201 acaaataatt ggagtcattc catgtttgaa aatagcgtgc gcttttttgt taatttcttc
    10261 atctgtttcg tggaataatt cacgacgttc agaatgaccg ataacaacgt atttaacgcc
    10321 taaatctgct aatgcaactg gagacgtttc acctgtgaac gcaccattat cttcgaaata
    10381 cgtattttga gtaccgattt ctaaaccttg tgcttttcct tctttaactg cagtagttaa
    10441 tgcatctaat tgaattgctg gtgcacaaat tactgattct acttcttttg aatctggtaa
    10501 tgttggtaat gcattgacga agtcttttgc ttcttgtact gttttgttca ttttccagtt
    10561 accagctata attggtgttc tcattaaaga cactccttgt tttgtaaata tttttgaaaa
    10621 gtgatgaaac acgatgtcat cttgtgactg ttttcccgta acaatgttaa acaaacatgc
    10681 cacatcactt taaactatca ctttattatt tattattgat tgctttgata ccaggcaatt
    10741 ctttaccttc taggtactct aatgacgcgc cgccaccagt tgaaatatga gtgaagtcat
    10801 tttcaaaacc taaagagatt gctgctgcag ctgaatcacc gccaccgata atcgtaattg
    10861 catctttaag gtttgcaatt gctttacata caccaattgt accttgtgca aagttactga
    10921 actcgaatac acccataggt ccattccata caacagtgtg cgcaccttct aattcatctg
    10981 caaataattt tacagtgttt ggtccaatat ccataccttc ttggtctgct ggaattgaat
    11041 cagatggtac tacagtgatt ttggcatcat tagaaaattc tttagcaact ttagtgtcta
    11101 ctggtaatac aattttatca ccatgttttt ctaataaatc ttttgcgaag tcgattttat
    11161 cttcttctaa taatgaaata ccaatttctt taccttgcgc ttttaagaaa gtataagcca
    11221 tacctccgcc gatgataatt ttatcagcta tgttaactaa gtttttgatg acattaattt
    11281 tgtcagatac ttttgctcca cctaaaatag caacaactgg tttatgtgga tcgttaacta
    11341 cgccaccaat aaacttaatt tctttatcca ttaagaatcc agctgcagtt tctaaatgtg
    11401 tagaaatacc aacattagat gcatgttcac gatgcgcagt accaaaagca tcatttacaa
    11461 acacatcacc taaagatgcc cagtatttac ctaattctgg atcattttta gattcttttt
    11521 taccgtctaa atcttcataa cgtgtatttt caactaataa tacgtcgcct tctttaaggt
    11581 ctttaatagc agcttcaagt ttttcgccgc gtgtttctgg tacgaaaaca acttctttat
    11641 ctaatttctt agataagtct tcagcaactg gacgtaaagt taattttgct ttatcacttt
    11701 cttctttcac tttacctaaa tgtgaaaata gtacgatttt accaccttgt tcgatgatgt
    11761 attgaattgt aggtaaagct tgaacgatac ggttgtcatt agtaatttca ccgtctttta
    11821 aaggtacgtt aaaatcagca cgtactagga ctgttttacc tttaagatct aaatcagaaa
    11881 caattttttt agccatggtg tttcctcctt gtaattagga aattttatat aaaaataagc
    11941 ggagaagcgt ttgtgctccc cgcttactca taagctttaa ttaatgctaa atagttctga
    12001 gcactagcgt atttgaataa aaactatact aaaattattt agaaagttca gctaagtatg
    12061 ctaatgtacg aactaattgt gcagtatatg acatttcgtt atcataccaa gctgcaactt
    12121 taactaattg acggtcgcca actgacatta cacgagtttg tgtagcgtcg aataatgaac
    12181 cgtaagtcat acctacaacg tctgaagaaa cgatttcgtc ttcagtgtaa ccgaatgatt
    12241 cgtttgaagc atttttcata gcttcgttaa cttgttcaac tgttacgtct tgtttttcta
    12301 atactactgt taattcagtt aatgaacctg tagctacagg aacacgttgt gcaccaccat
    12361 ctaatttacc atcgatttca ggaataactt taccgatagc tttagcagca cctgttgagt
    12421 tagggatgat gttttctgcc gctgcacgag cacgacgttt gtcacctttt ctgtgaggtg
    12481 cgtcttgtgt attttgatca cctgtgtaag cgtgaattgt agtcattaaa ccttcaacta
    12541 aaccaaagtc atcgtttaaa actttagcaa ctggtgctaa tgagtttgta gtacatgaag
    12601 cacctgaaac aactgtttca gaaccgtcta actcttggtg gttagtgttg aatacgattg
    12661 tttttaagtc accagtagct ggtgctgaga ttaatacttt tttagcgcct gcttcaatat
    12721 gagcttgtgc tttatcttta tcagtgtaga aaccagtaca ttctaacact acatcgatat
    12781 ttaagtcttt ccaaggtaat ttgcttgcat ctggttcact gaatgattta acttctttac
    12841 catttacgcg gaaaccacca tcaactacct ctacttcacc tgtgaaacga ccttgcatag
    12901 tgtcatattt taataaatgc gctaacatgt cgtcatctgt taagtcgttt actgctacaa
    12961 cttcaagacc ttctacttct tgaattcttc tgaatgctaa acgaccaatt ctaccaaaac
    13021 cattaattgc tacttttact gccattataa tggcctcctt taaaatgata tttaaaaagt
    13081 attaaacttt ttatctctta ttcaagtatt atctttgctg cggcttcatc agtgattaac
    13141 actgtattct tgggtgcaat cgtcaagtat gctttaattg cttcaccttt cgatttgcct
    13201 cctgcaactg caaaaataaa gtcttttgat tcaaggtctt ctaattgaag tccaattgtt
    13261 ttaaccttat ggacaatttg accttgtgta tcaaaataat aaccaaatgc ctctccgaca
    13321 gcttgatgat gttgaagttg ttcaatgacc ttttcaggtg attgacgtcg atgcgccatc
    13381 ttcagcgcat caccaatgcc gtgtaatata acgtttgctt gtttaatttt gtctaaagtg
    13441 tttatgactg atggctctaa caacaatgtg ttatatgttg tttcactgac attatcaggt
    13501 acatacatcg tcgtataata accgccagct tgttgtgcca tactggctgc aattgtgttt
    13561 gcctgaaaga caacattttc gcctagtcca cctctggctg gtacgaagaa tacattatat
    13621 ggtaataaat gaattgcttc actaacacat gccatcgtgg atccgccagt tacagcaact
    13681 atcgcgtctt cttgtaatat gccttcaagt aattgacctg cttgtctacc taattctgtt
    13741 ttaacagatt gactattatc agcatcacca ggaacaacat gaacttcctt aatttgaaat
    13801 ttattcttaa taccttctga cagacgatta tcatctgcat agatatcaaa gtaacctttc
    13861 aattgcgaaa tcagttgctc accttcagct gtaatttcca ttccggtagg cttaactttt
    13921 atcaaatctt gtttcttaag catatctgtt tcagaacgca gtacacgttc agtcatatcc
    13981 atatgttcgc ttaaacttcg acgtccgaca ggctgatttt ttgagatagt agtaagaata
    14041 gaaaaacgtt tatacatttt atctatgaga tccggtataa gcttttgctg tgcttgcaat
    14101 aagtctttca ctactacctc ctccttatat ttataaatgt aaataaggat atgtcacaac
    14161 cagtggttgc ctttttaagt cccgcgtggg acaaaaatta acctctttac acttgcaata
    14221 gtactattct atttcaaaaa ttgcaagcat aaaacttaaa ttttctaaat aatttgataa
    14281 agcgcttgca atacattttc gatttgttat tcaacaaaac gttttatttt attcgctgta
    14341 gtcaaatgta tacccttata ctgactcgat aatcatttgc ttattatttg agactaggac
    14401 ataaatcaat gctctagaat ccaaaaagtt acattagtag tagttgaccg aacgaaaatg
    14461 cacttgtaac aagctttttc caattaaagt caggggcccc aacacagaag ctgacgaaaa
    14521 gtcagcttac aataatgtgc aagttggggt gtgggcccca acatagtgaa attggctcct
    14581 ccca
//
LOCUS       DAHHYJ010000064        14311 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000064 DAHHYJ010000000
VERSION     DAHHYJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14311
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.064"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(113..2089)
                     /gene="sfaD"
                     /locus_tag="NBK22_001448"
                     /gene_synonym="sfnaD"
     CDS             complement(113..2089)
                     /gene="sfaD"
                     /locus_tag="NBK22_001448"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCD2414638.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNSDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSAPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSELGRTMSLSKDYDTTVHQLFPELIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNVQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPIVTL
                     IKRYQLAASLSDFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            2191..3384
                     /gene="sfaA"
                     /locus_tag="NBK22_001449"
     CDS             2191..3384
                     /gene="sfaA"
                     /locus_tag="NBK22_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCD2414639.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCALTYSFTAIPISVIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKIPEDATRFMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            3371..5128
                     /gene="sfaB"
                     /locus_tag="NBK22_001450"
     CDS             3371..5128
                     /gene="sfaB"
                     /locus_tag="NBK22_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCD2414640.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDIAYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKSEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDAQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDRARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYAQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            5135..6205
                     /gene="sfaC"
                     /locus_tag="NBK22_001451"
     CDS             5135..6205
                     /gene="sfaC"
                     /locus_tag="NBK22_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCD2414641.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNIQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMVDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(6306..6431)
                     /locus_tag="NBK22_001452"
     CDS             complement(6306..6431)
                     /locus_tag="NBK22_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414642.1"
                     /translation="MYHCYIIFALIQLTPFSMTVIPVIQLILSVAILCDELLHLI"
     gene            6597..7580
                     /locus_tag="NBK22_001453"
     CDS             6597..7580
                     /locus_tag="NBK22_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2414643.1"
                     /translation="MRGLKTFSILGLIVALLLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNINSFKTI
                     AKALNKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            7592..8623
                     /locus_tag="NBK22_001454"
     CDS             7592..8623
                     /locus_tag="NBK22_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD2414644.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIIHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFFIVFVTIIIPALEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWIIGALIV
                     TIFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            8620..9588
                     /locus_tag="NBK22_001455"
     CDS             8620..9588
                     /locus_tag="NBK22_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD2414645.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFTQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MTFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
     gene            9824..11194
                     /locus_tag="NBK22_001456"
     CDS             9824..11194
                     /locus_tag="NBK22_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCD2414646.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRILAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVIDFFKSGLKTVIDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(11326..11586)
                     /locus_tag="NBK22_001457"
     CDS             complement(11326..11586)
                     /locus_tag="NBK22_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414647.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            11763..12278
                     /locus_tag="NBK22_001458"
     CDS             11763..12278
                     /locus_tag="NBK22_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD2414648.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            12322..13509
                     /locus_tag="NBK22_001459"
     CDS             12322..13509
                     /locus_tag="NBK22_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCD2414649.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEEQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVRVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNKEGKDSVATATEDLRRM
                     GLI"
     gene            13528..14211
                     /locus_tag="NBK22_001460"
     CDS             13528..14211
                     /locus_tag="NBK22_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCD2414650.1"
                     /translation="MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLV
                     LPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
BASE COUNT         4749 a         1986 c         2613 g         4963 t
ORIGIN      
        1 tgtgattttt aataatttga atatggaaaa tgtattattc tctcatttgt atagattgta
       61 tttaataagt taatgtaatc cttgagctca cgattaataa aattctataa ccttaattat
      121 tttctcgata caaagggtta ttaactttaa tataagtata atgatgtgcc tcatcttcaa
      181 gacgcattgt tgtaacacac ttataatcta taaatggcgc gaacatggta tctttaattt
      241 catttatgcg attctcattt acttgattag atttgtgtgt cgaaagtaca agttgatcaa
      301 aaatgttatc tagtacatca cgaacgatat accacatatg tctttctaag tttgaatcat
      361 ttgatgcttt agagattgta agaattaatt cgcctaaatg gttttgaacg gtagaataaa
      421 aggctttgtt aaacacagac gtttttgaat cagtaagaat tcttgatttt tcatggaaat
      481 gagatgtact gtatcccatt tcatttagtt gtgctttatc aattcttaaa ccttcaaagt
      541 ctctaatata catcgtgtcc aatagaccat ctttccgaaa tgttgcaata gcattttgca
      601 agtgagcctc taatgcaatg ccatatttag taactagtgg gattacgaga ccaagcaatg
      661 ctttactata agtttcaatc cacgatttcg ccgatgattc aaaatcagac aatgatgcag
      721 ctaattgata acgtttaatc aatgtcacga taggtgattc attgttaaat gggtaggttg
      781 caactaagct tgaaggaatc attggtgtga cttcttgtgg aatcatttgg tatatgtttt
      841 ttctaaataa tgtacctaat tgttcacttc gttcagtttg atagtctgct tcgtcttgca
      901 cattataaaa atgaatacca gcaacttcat caataattgt tgatgcatag gacttaaata
      961 tgacatcttt ctccaaaata tcatttaaaa tacgtgtcat tagtggacca ttgtgcgtcg
     1021 tttgttctga taatgtacga atttcacctg taatatgaac gtttgtcgac aatttgatgt
     1081 gtggcgacat agctgggtat ttaggaacta atgttctgaa agataaacca gcataataat
     1141 ccaacgtatg ttttgcttca atgattaatt ctttatctac ttctgcttga taatcagaat
     1201 gtaatacgtc atctaattgc catggatgaa caatcataat gtgataatca ttaaagttaa
     1261 actttggcgt aaattcattt tctaattgtt taattaattc cggaaatagt tgatgaacag
     1321 ttgtatcata atctttagac agtgacatag tccggcctaa ttcactgtga atcagtacta
     1381 ttttcaactt aataggttga ttgaattctg aagaatatag gaatgtttgt aatgcattta
     1441 aacctttacg taatttagcc ccaggatgta gcggatgacc ttcaataacg gcttgctctg
     1501 aacgcaagta actatcttcg ctattttcga taatattaaa taaaggtgca gaatcatgtt
     1561 gcattgacag tgcttgatag ctaattgcaa atgtcatatt agttgcactg tttattaaat
     1621 cttgctgaaa ttgatcacta gcagcatttt ttaaatctgg tgcttcaatt aaaatacact
     1681 caagaatttc atttggatgg tgtactcgtg taatcgtatt tgtaatgtca tctttaatgt
     1741 agaaagggcc ttcaacatca attcgatcaa aggcgtgttc tccagtgata ggagcatata
     1801 atgtttgctt agcttgtggg aactggattt ctagtatatg agtcgttgag atatctaaca
     1861 taatcaaatc acgactcaat attttcttac tttgagtgcg cgctttaact aagttttcgc
     1921 gatgcattga tgtgactaat ctctgagtga ctttatctct tccttgtaaa atcatctctt
     1981 taaaaatatt agcccaatcg ctattatgtt gttgtaaaaa taaatatgtt tcttgttctt
     2041 ctttgttaaa ttttaatgtc tgttctttaa aaattaagtt caagttcata attcacctct
     2101 atgaaatatt ttacaaaagc aagatagatt tgtataatcc atattaatga taatgattct
     2161 tattatcaac agaatgcggg tgtaagtttt atgacaaaat atttttttag cagttctttt
     2221 ctactatttc taggtaattg gattggacaa ataggtctaa attggtttgt gcttaccact
     2281 tatcataacg cagtttatct ggggattgtc aatttttgca gacttgtacc aatattatta
     2341 ctaagtgtgt gggcaggggc aattgccgat aagtatgata aagggcgatt gctgagaatt
     2401 acaatttcat catcattttt agtaactgca attttatgtg cgctcacgta tagtttcact
     2461 gcaattccaa ttagcgtcat tattatatat gcgacattaa gagggatttt aagtgcggtt
     2521 gaaacacctt taagacaagc aatcttacca gatttatcag ataaaatatc tactacacaa
     2581 gctgtatcat ttcattcatt catcattaat atttgtcgtt caatagggcc tgccattgct
     2641 ggtgtcatat tagcagtcta tcatgcgcca acaacatttc ttgcacaggc aatttgttat
     2701 tttatcgcag ttttattatg cttaccatta cattttaaag taactaaaat acctgaagat
     2761 gcaacaagat tcatgccgtt aaaagttatt atagattact tcaaattaca tatggaaggt
     2821 cgacaaatat ttataacatc attattgatt atggcgacag gtttttcata tacgacactt
     2881 ttaccagttt tgacaaacaa agtatttccg gggaaatctg aaatatttgg tatcgctatg
     2941 acgatgtgtg ccattggtgg tattattgca acgctagttt tacctaaagt acttaaatat
     3001 attggtatgg taaatatgta ttatttaagt tcatttttat ttggcattgc tttgttaggt
     3061 gtggtatttc acaatattgt catcatgttc atttgtatta cattgattgg gttatttagt
     3121 caatgggcac gtacgacaaa tcgcgtttat tttcaaaata atgttaaaga ttatgaacgt
     3181 ggtaaagtac tgagtattat tatgatggat agaggtatga ttccattggg aagtctatta
     3241 atgagtatat gtgcagatgt gtttggcatt gttagaactt tttcaataat gggaataagt
     3301 actatatgca ttacaatggt attctatttt ataaatagaa agttgaagtt aaagttggag
     3361 gaaagtaatc atggtatatc ttgaatgggc aaaggcagat agaaatattc aatatcgtgt
     3421 aattaacgcc attattaaag aacgtattta ccccgagcaa acatttattt cgcaaaaagg
     3481 atctttaatt gaaattcagt atcatatgca tgtgttgact attgaagttg ttagaaaaag
     3541 tgcattagaa cgctatgagt ttacaggtga tattgcttat ttaaataaag gtgaaacgtc
     3601 attaattata actttagaag gtttgttaga tgtgttgaat catgactttg atatccctat
     3661 ttcagagcga ctacgcgaag agttaataca cagtcgagat agtttagttg aaacatataa
     3721 gcaaatgtct cacagacaaa cattaataag tcaaagtttt aaattttcaa gattaccaca
     3781 agatattaac tttttttcat ggttacaaca tgtaaaagat agtgataaga cagatgattt
     3841 aacttattct gagagcttgg taccagaggg gcatccaaca caccctttaa ccaaaacgaa
     3901 attgccctta actatggaag aagtacgagc atatgcacct gagtttgaaa aagaaatccc
     3961 tttgcaaatt atgatgattg aaaaagatca tgttgtgtgc acagctatgg atggtaatga
     4021 tcaatttatt attgatgaaa taattcccga atactacaat cagattcgtg tgtttttaaa
     4081 gagtttaggt ttgaaaagtg aagactatag agcgatttta gtacatcctt ggcaatatga
     4141 tcatacgata gggaaatatt ttgaagcatg gattgctaaa aaaatattaa ttccaacgcc
     4201 gtttacaata ctttcaaaag caactttatc atttaggacg atgtctttaa ttgataaacc
     4261 ataccatgtt aagttgcccg tcgatgcaca agcaacaagt gccgttagaa cagtctcaac
     4321 tgtgactact gtagatggac caaagttaag ttatgcttta caaaacatgt tgaatcaata
     4381 tccaggattt aaagttgcta tggaaccgtt cggtgaatat gcaaatgttg ataaagatag
     4441 ggcacgtcag cttgcttgta ttataagaca aaagcctgaa attgatggaa aaggtgcaac
     4501 agtagttagt gcaagtctag ttaataaaaa tccaatagat caaaaagtta tcgtggatag
     4561 ttacttagag tggttaaatc aaggaattac taaagaaagt attacgacat ttattgaacg
     4621 atacgctcaa gcattaatcc cgcctttaat tgcttttatt caaaattatg gaattgcatt
     4681 agaagcacac atgcaaaata cagtagtgaa cttggggcca cattttgaca ttcaattttt
     4741 agtgagagat ttaggtggtt ctagaattga tttagaaaca ttacaacatc gtgtatcaga
     4801 tattaaaatt acaaatgata gtttaatagc tgattctata gatgcagtga ttgcaaaatt
     4861 ccaacatgct gttattcaaa atcaaatggc agaattaatc catcatttta atcagtatga
     4921 ttgtgttgaa gaaaccgaat tatttaacat agtacagcaa gtagtagcgc atgctattaa
     4981 cccaacacta ccacatgcaa atgagttaaa agatattttg tttggaccaa caattactgt
     5041 caaagcgttg ttaaatatga gaatggaaaa taaagtaaag caatatttaa atattgagtt
     5101 agataatccg ataaaaaaag aggtgtagta ctacatggca cacgttaaca taaatatatc
     5161 gaagattaaa tataacgcca aagtacttca aacagttttt caaagtaaaa atatacaatt
     5221 cacaccagta attaagtgta tagctggtga ccgtacaatt gtagaaagct taaaagcgtt
     5281 aggtatcaat catgttgcag aatccagatt ggataacata attagtattg cagatcagga
     5341 tttaacatat acgttattgc gtacgcctgc caaaaaagag atttcagata tgatagaaaa
     5401 agttgatatg agtatccaaa cagaattatc taccatacat caaataaatg aagtagcaga
     5461 agttttagga aaaaaacata agattttatt aatggtagat tggaaagatg gccgtgaagg
     5521 tgttctgact tatgatgttt tagattatat taaagagatt attcacttga aaaatatcca
     5581 ttttgtaggt ttagcattca attttatgtg ttttaaatct gatgcgccaa gtgatgatga
     5641 tatctttatg attaatcgat tcgtctcagc tgttgaaaga gaaataggat atcgattgaa
     5701 aattatttca ggtggtaatt cgagcatgtt accacaactt ttatataatg atttgggtaa
     5761 aataaatgaa ttgcgaatag gtgaaacgtt gtttagaggt gttgatacaa cgacaaatca
     5821 agcgatcgct atgttatatc aagatgcgat tacattggag gctgagattt tagaaattaa
     5881 acctcgagtt aatacacaaa cgcatgaatc attcttacaa gctattgtag atattggtta
     5941 tttagataca aaagtcgata atatttcacc tatggatcag catatcaata ttttaggcgc
     6001 ttcgagtgat catttaatgc ttgatttaaa tggtcaagga cactatcaag ttggagatca
     6061 tatatctttt tcattaaatt atgaagcact ttctcattcg atgtatatga aaaatttaca
     6121 taaggtatat attgatgatt caaagattga tacgctactt caaaattttg atgtgaaatc
     6181 tcctgctatg gtaaaccaat attaatatat ttatcaataa gtctaagttg acaagttata
     6241 ctacatatcg tttgcacggt tgtatcataa ttgttcaact tagatttttt gtatttgttg
     6301 atttatcaaa ttaagtgcaa cagttcgtca cataaaattg caacagataa tatcagctga
     6361 attacaggga taacggtcat gctaaatggt gtcaattgta ttaatgcaaa gatgatatag
     6421 caatgataca ttatcagtat tttgtctaag gaaatgtgct aattgtagtc ataattatta
     6481 gaggaaatat atagtcatac attttagaaa tataaaaaag attgaacgct acttgacaat
     6541 gataattgtt atcaataaaa taataaatga agttatacat attaaggagt ggaacgatga
     6601 gaggtctaaa aacttttagt atattgggat taatagttgc cttactttta gttgcagctt
     6661 gtggtaatac ggataattca agtaaaaaag aatcatcaac taaagatact atttcggtaa
     6721 aagatgaaaa tggtacagta aaagtaccta aagatgcaaa acgtatcgtt gtattagagt
     6781 actcatttgc agatgcatta gcagcattag acgttaaacc agttggtatt gctgatgatg
     6841 gtaagaaaaa acgtatcatt aaaccagtta gagaaaaaat tggggattat acttctgtag
     6901 gtacacgtaa acagccaaac ttagaagaaa ttagtaaatt aaaaccggat ttaattatcg
     6961 ctgatagcag tagacataaa ggtattaata aagaattaaa caaaattgca ccaacattat
     7021 cattaaagag ttttgatgga gactacaaac aaaatattaa ttcgttcaaa acaattgcta
     7081 aagctttaaa taaagaaaaa gaaggcgaaa aacgtcttgc tgagcatgat aaattaatca
     7141 ataagtataa agatgaaatt aaatttgata gaaatcaaaa agtgcttcca gcagtagttg
     7201 ctaaagctgg tttattagca catccaaact attcatatgt tggacaattt ttaaacgaac
     7261 taggatttaa aaatgcatta agtgacgatg taacaaaagg tttaagtaaa tatttgaaag
     7321 gaccttactt acaattagac actgaacatt tagctgattt aaatccagag cgtatgatca
     7381 ttatgacaga tcatgctaaa aaagattctg ctgaattcaa gaagttacaa gaagatgcaa
     7441 catggaaaaa gttgaatgca gttaaaaata atcgcgtgga tattgttgac cgtgatgttt
     7501 gggcaagatc tcgtggctta atttcttctg aagaaatggc taaagaactt gttgaattat
     7561 caaaaaaaga acaaaagtaa ggtggaagta aatggctata aaagaaataa gtagccaatc
     7621 tgccatagat cataaaagaa aaagacgcac aacactcacg tatatagtga gtttgtgctt
     7681 tctttttatt tgtatatatt taaatatggc gattggttct tcgaaaatta attttagcga
     7741 tatcattcac tatgttactg gtcatacaga tacgaaagca acgtttttat tgcataatgt
     7801 acgtatgcca aggatgattg cagggttatt tattggcggt gcattagcgg tatctggttt
     7861 gttaatgcaa gcaatgacac gcaatccgct agcatcacct aaaatttttg gtgtcagttc
     7921 tggtgcatcg tttttcattg tatttgtgac gattatcatt ccagcattag aatattatgc
     7981 attatattta ggtgtgatag gtgcatttat aggtggttta actgtttata cactttcagg
     8041 tgctacgaaa ggtatgacac ccattaaatt agcattggct ggtatggcca tccatttgtt
     8101 ttttagtagt atgaccgaag gtattattat tttgaatgag aattcaaacg aacaagtgat
     8161 gttttggctt gtcggttcat tatccagtat gaagtgggat gaaattttaa ccattctacc
     8221 atggattatt ggtgctttaa ttgtaacgat ttttattgga cgtcaactaa cgataatgga
     8281 actaggcgat gacattgcta agggattggg tcaaaatata aataaagtta gaattatcat
     8341 tggactatta gtcattattc ttacaggtat gtcggtgtca gtggcaggac caattggatt
     8401 tgttggctta atcgtaccgc atatagtcaa acgctatgtg agtaagaatt atttagtcat
     8461 gattccatta acatttatta taggtgcaga tttgttacta ctatcagatg tattaagtcg
     8521 tttaattaca tatccttatg aatcaccagt agggattgtt acatcattcg taggtgcact
     8581 ttacttctta tttataacga ttaaaggggt gaaaagaata tgatgacaac gaaaattata
     8641 cgtcgttata gtttaatcac aatcctttta attgtgagta tattcataag tttatgtgta
     8701 gggtctgtca tgattcatcc gattgatgct attaagggaa tctttacaca agatgatttt
     8761 attttaaatg agtatcgtat acctagaaca ttacttggta ttattatcgg tagtagcttg
     8821 gctatttcag gtgcaattat acaagctgta ataagaaacc cgttagcctc acctgatgtt
     8881 ataggtattt ctaaaggtgc cagtttagct gctgtgatta ttattatgac ctttccaaca
     8941 gcaccattgt ttgtattacc tataggttca ttcttaggtg cctttgcagt aagtttattt
     9001 ttatcatttt taatttcaaa atatgatgtt aaaggttcaa agcttgcctt aattggttta
     9061 gcaataggcg caatatgtac ggcgattgtg cagttcttat taatcagaaa tccactggaa
     9121 gcaaacaaag cgcttgtttg gttgacaggt agtctatatg gacatgatat gtcgaatatt
     9181 tatactatat taccttggtt tttaataacg ataccaatca tcattttatt gagttatcaa
     9241 ttagatgtat tgaatctagg tgatgctgtt gcaaccgcac tcggtttaaa agttaaaact
     9301 attaaaatgc tattgttaat tttagcagta atattagcag gatccgcaat ttcagttgtc
     9361 ggtggtatta gctttttagg attaattgca cctcatattg cgagacaatt aataggaaat
     9421 aaaacattac acgttattat catgtcgggg ttaattggtg ctatattgtt aacatttgga
     9481 gatggtttag cccgtggcat tcatccgcca ctggatattc ctgttggggt tatcattgca
     9541 attatcggtg caccatactt cttaatatta ttacgaagaa tgaaataact aacatatgat
     9601 tagagtttaa aaaaggttaa gtacgaaatc agtaattgta cttaaccttt tttgaattca
     9661 tcttgaaaaa ttacaagaaa tagcgtatat ttaataactg taattattca ttattttgca
     9721 tacataaaca ttttgaaaga tagaatgata aggaatatgt ttgttaactg aatagaattc
     9781 tgtatatact taaaacgaat acatagagaa atggattgat tttatggata gcttttcaag
     9841 gaaagagata gttattggga gacttaagtt tattacgatg tctttaattg gtatcttatt
     9901 atttttagta ccaattccag tcgaacaaga tggacagaag caaacaacgt tgcccgtggc
     9961 atttttagca ggcgtgctta aagatgtatt aggtggcgta atgccatttt taattgtgac
    10021 gataattact ttatcgggta tcataactct gatttgttca actattttaa aagataaatt
    10081 gaaacctgac gggctaatga acaatgcttt taacgttaga attggttggt taattttacg
    10141 aatattagca gttgtttttg catggatgac atttttacga attggttcaa aggttattta
    10201 ctcagatgaa acgggtggat tattgttctc aagtttattg ccaacattag tggcagtatt
    10261 cttatttgca gctctatttt taccactatt aatggaatac ggtttgttag agatgttggg
    10321 tcctattttt agaccggtta tgagaccttt atttacattg ccaggacgtt cgacggtaga
    10381 taatttagca tcatttatcg gagacggaac tgttggggtc ttaattacaa gtagacaata
    10441 tggtgaagga tattattctc gtcgtgaggc aacagttatt tcaacgacat ttagtgtagt
    10501 ttctatcacc tttgcaattg tagttgctga aacagtgcac atgcaaaatc aattttttgc
    10561 tttctattta tcagtgatag tatcctgcct cgttgcagca gtcattatgc caagaatttg
    10621 gcctttaaat aaaattcctg atgagtatgc taaggaggta cctgaaagtg cgcgtactga
    10681 ggcattgcca gaaggaaaga cggcattgag acatggcttt gatactgcaa cagaagtagg
    10741 tattaaagca ccgggcgtta ttgacttttt taagtcagga ttaaaaacag tcattgatat
    10801 gtggtttgtt attttgcctg tagtaatgag tattggtact attgctacca ttattgcgaa
    10861 ctacacgccc ttttttgtca tattgggtaa accttttgtt ccatttttag agttaatgca
    10921 aatacctgaa gcggcacagg cttctcaaac gattattatc ggttttgccg atatgttttt
    10981 accttctata ttaattgaag gtgtacaaaa tgatataact cgttttgtta ttggggcact
    11041 tagtatatcg cagttaatat acttgtctga agtaggtggc gtcatacttg gttcgaagat
    11101 tcctgtaagc atcggcaaat tgtttatgat tttcttgatt agaacaatca ttacactacc
    11161 aattatttct ttgatggcac atcttttgtt ataaatatat gtgaaaaagg acaactgtta
    11221 aaattatggg aacagttgtc cttttgtatt tgtttatgct gggtgttgaa tcaattaaaa
    11281 aaatcattca tgaagccctc accatgaatg atttttttat acaatttatt tagttaattc
    11341 attgtaaact ttttgatcat tttgagtata aataatgtat tcgtggtctt cagtatcgat
    11401 aataacgcga ttcgttttac caaacgttga gccgattcta gatacatctt tgctgtctaa
    11461 tttaggaact gcatgaatat cttggtcttg tgtaatattt ttaatttcac ttgtaggaat
    11521 tttgatatca gcaactctcc attgaatacg cacttcatta ttatcctttt ttactgtcat
    11581 tgccattatt caaacacatc ctttaaatat tttgtaaata catcataaac gattttgtaa
    11641 tcgttttcaa gagttatgtg atactttttt aaattgtgtc atactaatga gttttaaccc
    11701 gaaaatatgt tcgtgttttt gttagggatg tggtaaaatg tgagtattaa aggagaacaa
    11761 atatgacagg taaaacacat gcttcatgcg gcatgctcgt tggagcgcta acaacacaat
    11821 attttcaaac ggatattttt tcgtcggtta cggttatcat tctagcaact ctagctagct
    11881 tattacccga tatatgtcac actcaaagta agattggcag aaggtttaag gtaattagtt
    11941 tttttgtgag attgatcttt ggacatcgaa cctttacgca ttcaatatta tttatcgcaa
    12001 tcatagcatt tttattgcaa attattcaga cgcctaatta ttatatggca gcaattataa
    12061 ttggattagt gtcacatgtt atactagata tgataacacc aaaaggcgtg aaattatttt
    12121 atccgttacc gtttaatgtg aagacaccga ttcaatttaa aacaggtgga cttgtagact
    12181 tatctttagc tacagcgtta atggtcggaa cagtttatgt tttatttcag cctttcatca
    12241 atgatataat aagcaactgg aatactaagt ttttatagaa aataaattat taaaggttac
    12301 aaactcagca ggaggcataa aatgctagat aaaaatcaat tagctaaata taaacaagat
    12361 catttgtgtg aatatgaaaa aataatgagt aacaatgaaa aagaagcact tgaagagaag
    12421 gtagcgtcat tagatttaga ttttattgcc aaattatata acgatttata catcaataag
    12481 aaaacaatag atgatgtatc tgcagtttct gaagtgaaat atgatattaa atcacaaatg
    12541 agtgatgatg agattaagcg tttagaggaa caaggacttc aagctattaa agaaggacaa
    12601 tttgcagtac ttttgatggc aggtggtcaa ggaacaagac ttggttacaa gggccctaaa
    12661 ggttcgtttg aaattgaagg tgtaagttta tttgaacttc aagctaacca attaaaaaca
    12721 ttgaatcatc aatctggaca tacgattcaa tggtatatta tgacaagtga tatcaatcat
    12781 gaagaaactt tagcttattt cgaagcacat agttattttg gatatgatca agaagcaatc
    12841 catttcttta aacaagataa tattgtggcg cttagtgaag aagggaagtt aattttaaat
    12901 caacagggtc gtattatgga aacgccgaat ggtaatggtg gcgtgtttaa atctttagac
    12961 aaggcaggat acttagaaga aatgtctaat aatggcgtta agtatatttt cttgaataat
    13021 atcgacaatg ttttagtaag agtgttggat ccactatttg caggatttac ggtagaacat
    13081 gattacgata ttacttctaa aacgattcaa ccaaaaccag gagaaagtgt tggacgttta
    13141 gttaatgtcg attgtaaaga tacagtactg gagtattcag aattagatcc agaagttgca
    13201 aatcaattta acaatgctaa tatcggtatt catgcattta aacttggttt tattttaaac
    13261 gctgtaaatc gtgagttacc ttatcattta gctattaaga atttgaagca attagatgag
    13321 aactttggtg ttattgaaca accaacttta aaattcgaat tgttctattt tgacatattt
    13381 acttatggaa ctagttttgt cacgttacaa gttcctagag aagaagaatt ttcacctctt
    13441 aaaaataaag aaggtaaaga tagtgttgca actgcaacag aagatttacg tagaatgggt
    13501 ttaatttaat gataggcggt gactatgatg tccaaatcaa ctaaaaaaag taatgacaca
    13561 atcgtagaaa catttaagga tatcatccct ttatcatttg gagaagaaat tggtaatgcg
    13621 gcatctcatg gtgttgcagc tttgttaaca ttattagtgt taccctatgc ggctgtgcac
    13681 agttatattg cacatggtac gcttgcgtct gtgagtatgt ctatttatgt tatttctatc
    13741 tttatgatgt ttatttcatc tactatttat cattctatgc aaaatgaaac acctcataaa
    13801 tatattttaa ggattatcga tcacagtatg atttacgtag caatttctgg tacatatacg
    13861 cctattttgt taacagttgt aggtggttgg ataggttgga cagtatttat tttattatgg
    13921 ggaacgacac tttgggggat tttatataaa tccatagctg tcaaagttaa ccaaaagtta
    13981 agcttaattg tctatttagt gatgggctgg gtaggtatta tcttgttacc aattattttt
    14041 attcgcacat catggatatt catattattc attttcttag gtggtgtgtc ttatacaatt
    14101 ggtgcatggt tttatgcaca aaagaataga ccatattttc atatgatttg gcatattttt
    14161 attgtaatag catcactatt gcatttaatt gcaatcttat attttatgta gtcgtttaga
    14221 aaagctgggg gaaactccgc tttttttatt acataaatca ttctaatagg ggattgtatt
    14281 gtaatagaat tatcgttaag atttgaaagt c
//
LOCUS       DAHHYJ010000065        14267 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000065 DAHHYJ010000000
VERSION     DAHHYJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14267
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.065"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(33..479)
                     /gene="perR"
                     /locus_tag="NBK22_001461"
     CDS             complement(33..479)
                     /gene="perR"
                     /locus_tag="NBK22_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCD2414651.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(576..1526)
                     /locus_tag="NBK22_001462"
     CDS             complement(576..1526)
                     /locus_tag="NBK22_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD2414652.1"
                     /translation="MKVVGLNRMREVETELQQRFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLQRCPNLKWIAWFATGVNTLPLDYIADHGILLTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKEAVLIEVLKSKVIRHAYLDVFENEPLKPNHELYELENVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(1532..1987)
                     /gene="bcp"
                     /locus_tag="NBK22_001463"
     CDS             complement(1532..1987)
                     /gene="bcp"
                     /locus_tag="NBK22_001463"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCD2414653.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2068..3357
                     /locus_tag="NBK22_001464"
     CDS             2068..3357
                     /locus_tag="NBK22_001464"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCD2414654.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            3650..4744
                     /locus_tag="NBK22_001465"
     CDS             3650..4744
                     /locus_tag="NBK22_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD2414655.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(4834..4926)
                     /locus_tag="NBK22_001466"
                     /pseudo
     CDS             complement(4834..4926)
                     /locus_tag="NBK22_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4935..6671)
                     /locus_tag="NBK22_001467"
     CDS             complement(4935..6671)
                     /locus_tag="NBK22_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
                     /protein_id="HCD2414656.1"
                     /translation="MIKRYLQFVKPYKYRIFATVIVGIIKFGIPMLIPLLIKYAIDGV
                     INNHALTTDEKVHHLTIAIGIALFIFVIVRPPIEFIRQYLAQWTSNKILYDIRKKLYN
                     HLQALSARFYANNQVGQVISRVINDVEQTKDFILTGLMNIWLDCITIIIALSIMFFLD
                     VKLTLAALFIFPFYILTVYVFFGRLRKLTRERSQALAEVQGFLHERVQGISVVKSFAI
                     EDNEAKNFDKKNTNFLTRALKHTRWNAYSFAAINTVTDIGPIIVIGVGAYLAISGSIT
                     VGTLAAFVGYLELLFGPLRRLVASFTTLTQSFASMDRVFQLIDEDYDIKNGVGAQPIE
                     IKQGRIDIDHVSFQYNDNEAPILKDINLSIEKGETVAFVGMSGGGKSTLINLIPRFYD
                     VTSGQILIDGHNIKDFLTGSLRNQIGLVQQDNILFSDTVKENILLGRPTATDEEVVEA
                     AKMANAHDFIMNLPQGYDTEVGERGVKLSGGQKQRLSIARIFLNNPPILILDEATSAL
                     DLESESIIQEALDVLSKDRTTLIVAHRLSTITHADKIVVIENGHIVETGTHRELIAKQ
                     GAYEHLYSIQNL"
     gene            complement(6963..7505)
                     /locus_tag="NBK22_001468"
     CDS             complement(6963..7505)
                     /locus_tag="NBK22_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD2414657.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
     gene            complement(7807..8844)
                     /gene="mutY"
                     /locus_tag="NBK22_001469"
     CDS             complement(7807..8844)
                     /gene="mutY"
                     /locus_tag="NBK22_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCD2414658.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVHDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARRKMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            8996..9973
                     /locus_tag="NBK22_001470"
     CDS             8996..9973
                     /locus_tag="NBK22_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD2414659.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            complement(10234..11070)
                     /locus_tag="NBK22_001471"
     CDS             complement(10234..11070)
                     /locus_tag="NBK22_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414660.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIVLIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCFLLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(11079..12596)
                     /locus_tag="NBK22_001472"
     CDS             complement(11079..12596)
                     /locus_tag="NBK22_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414661.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDYKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            complement(12611..12925)
                     /locus_tag="NBK22_001473"
     CDS             complement(12611..12925)
                     /locus_tag="NBK22_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCD2414662.1"
                     /translation="MEQKKLSEMSELELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            complement(12903..13721)
                     /gene="recX"
                     /locus_tag="NBK22_001474"
     CDS             complement(12903..13721)
                     /gene="recX"
                     /locus_tag="NBK22_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCD2414663.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            complement(13982..>14267)
                     /gene="sgtB"
                     /locus_tag="NBK22_001475"
     CDS             complement(13982..>14267)
                     /gene="sgtB"
                     /locus_tag="NBK22_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCD2414664.1"
                     /translation="YLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAIL
                     ASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
BASE COUNT         4645 a         2449 c         1979 g         5194 t
ORIGIN      
        1 cttttttaat ttgtcatact accaaagtta aattatttat cttggcattc tttacaaact
       61 ccataaattt ccattcgatg atgtgttacg tcaaagtcag tcatatgctg agctaatctt
      121 tcaatttcat ttaactgtgg atattgaaaa tcaacaatct taccacattg ttcacatata
      181 atatgataat gattatgtgt attaaagtcg aatcgacttg atgagtctcc atatgttaat
      241 tcttttacaa ttccaatatc tttaaacact cttaagttat tatatattgt cgcaacactt
      301 atatttggaa aatcaggtga aagtgcttga taaatttcat cagctgttgg atgagtatgt
      361 gaagaaatta aataacgtaa tattgcttgt ctttgaggtg taattcttac gcctgcttgt
      421 cgcaatgatg caattgattc ttctagttca tgttcaattg attctatttc aacactcatc
      481 tatatcacca tctttcttat ataataatta ttattactta ctataaatat aactgtagta
      541 ttgcgcgcgt gtcaatattt acatgatgat ttcatttaat agcctttttt agcatcaact
      601 tcattctcaa ttagaccatt cttattgaga aaattaacta gattgttttt aaaaatatct
      661 aataagtcat actttgcctc ataatcatta ccagttatat gcgctgttat agttacattt
      721 tccaattcat ataattcatg attaggtttc aaaggttcat tttcaaacac atctaaatat
      781 gcatgtcgaa taactttact ttttaatact tctattaaga ccgcttcttt aactatgcta
      841 cctcgtccta tatttataaa aagtgcttca tctttcatta attcaaaatg ttttttcttt
      901 agtaaatgaa tcgtttcttg cgtttctggt aaagcattta taataatgtc agcatttggt
      961 aatgtgcttt caagtgattc aatagtatat atctcgtcaa actcatcttt gttctgacct
     1021 gacttgctca gaccaattaa attcatatta aaagcctttg ctaacttcgc agttctagta
     1081 gcaattgcac ctgtacctaa aaataaaact gtttgtcctg ataggcgttt accagttatt
     1141 ttcgaatcat atatatgttg tcgttggtta tcatatgata gtttcatctt tttataatca
     1201 tctaaaatga aagctaaaat gtattcagat aattgtttag cttgaacacc ttttccatta
     1261 gttaaaagta tgccgtgatc tgcaatataa tctaacggca atgtatttac acccgttgca
     1321 aaccatgcaa tccattttaa attcgggcaa cgttgtaaaa atgcctcatt gataccgcca
     1381 tcataaccaa ctaatatatc taaatcagcc aagtcactct caggtatttc tgatgctttt
     1441 ttataaaatt taaaatctaa atctgaaaag cgttgttgta attcagtttc aacttcacgc
     1501 atacgattca acccaacaac tttcatatat gtcaccccaa aatgttttta agttcttcta
     1561 tttgtgtttt aaccttaacc ttctcgataa catctaatac tttaccttgt tcatctatta
     1621 taaaagtcgt tcttacaatg cccatacttt ctttgccaaa tgattttttt aactgatata
     1681 cgccagtttc tttagctaat ttaaaatctt catctactaa taaatcgaaa ttcaatccgt
     1741 gtttctcaat aaaattttgg tgttttttct ttgaatcacc gcttatacca tatactgcaa
     1801 catctaaatc attgaacatt tctaaattgt ctctaaagtc acaagcttct gtggtacaag
     1861 taggtgtatt atctctagga taaaaatata taatcgcctt tttacctttt aatgtatcat
     1921 ttgtaatgac agttccgtct tgattttcta atttaaatat tggaaattgt tctccttttt
     1981 gcaacatata atcacctttt tcttcattat aataatttta tgatacgata ttagatgaaa
     2041 atattaaatt aaaagaggtt gatataaatg aattttagtg aaagtgaacg tttacaacaa
     2101 ctttcaaacg aatatattct aggcggtgtc aattcccctt ctcgttctta taaagctgta
     2161 ggaggcggtg cacctgttgt tatgaaagaa ggacacggtg catatttata tgatgtcgat
     2221 ggcaataaat ttattgatta ccttcaagca tacggtccaa ttattacggg gcatgcacat
     2281 cctcatatta ctaaagcaat tcaagaacaa gctgctaaag gtgttttatt tggtacaccg
     2341 actgaattag aaattgaatt cagcaaaaaa ttacgtgatg caattccatc tcttgagaaa
     2401 attcgctttg taaattctgg aacagaagca gtcatgacaa caattcgtgt tgcacgtgca
     2461 tatactaaaa gaaataaaat tataaaattt gctggatctt atcatggcca ttctgattta
     2521 gtattggttg cagcaggtag cggcccatct cagctcggtt ctccagactc agctggtgtt
     2581 ccagaaagcg tcgcacgtga agtcattact gtacctttca atgatattaa cgcctataaa
     2641 gaagcaattg aattttgggg tgatgaaatt gccgcagtat tagtagaacc aattgttggt
     2701 aactttggaa tggtaatgcc tcaacctgga tttttagaag aggttaatga aatttcacat
     2761 aacaatggga ccctagtgat ttatgatgaa gtaattactg cattccgttt ccattacggt
     2821 gccgctcaag atttattagg tgttatccct gatttaactg catttggtaa aattgttggc
     2881 ggtggtttac caattggagg ctatggtgga cgtcaagata ttatggaaca agtagcacct
     2941 ctaggacctg catatcaagc tggtacaatg gctggtaacc cgttatctat gaaagcaggt
     3001 attgcattac tcgaagtact agaacaagac ggtgtttatg aaaaattaga cagcttaggc
     3061 caacaactag aagaaggttt acttaaatta atcgaaaaac ataatatcac agctacaatt
     3121 aatcgtattt atggatcttt aacattgtac tttacagatg aaaaagtcac acattatgat
     3181 caagttgaac attctgacgg cgaagcgttc ggtaaatttt tcaaattaat gttaaatcaa
     3241 ggtatcaatt tagcaccttc taagtttgaa gcttggttct taacaactga acatacagaa
     3301 gaagatatta aacaaacttt aaaagctgca gactatgctt ttagtcaaat gaaataactt
     3361 ttaaggaagt atatttttaa ttgaattaaa ataaaacaaa tgctcacata gtagattcat
     3421 tttatatcgt taactattaa cgtttaactt gatatgtcac tatgtgagtt tttattttac
     3481 aaatttatac tcttttaatc tctgactaaa gttttacatc gctatcagta ttatgtatga
     3541 tttattttaa caactataaa taagcttgaa ttttgtaact agtaagtgta taacagtaat
     3601 gaatacgaat attagatatt caataaactt aaaaggggcg tcattatttt tgagactagg
     3661 agctcggatt tttaaaactg gtatagccat tattttagct atgtctatcg cttctttact
     3721 accggatgat gttggtctga aagccttagc tggtgtcagt gctgttgttg caatgcaacc
     3781 tagtatttat cgttcattta aaacagtttc tgatcaagca ttgggtaaca ttataggtgc
     3841 catattatct gttacaatgg taacaatttt tagtgataat tttatcatta tgggtgttac
     3901 cgtcatagta cttattgcta ttttatttaa atttaattta gcacatgttg ctactttggc
     3961 gagtgtaaca gcattaatca ttatgggaca acataccggt tctttctata ttaccgcctt
     4021 ttacaggttt gtacttgtaa tgattggtgt cataagttca tctctagtta actttgtgtt
     4081 tctaccacct aaatttgaaa caaaaattta ctataattca ttaaatatat cctcagatat
     4141 ttttatgtgg tttaaattag tattaaatga tacgacggag tttaataata ttaaacaaga
     4201 tagtcataat ttaaaacaac gtgtcgaaaa gttagagaaa atttatgatt actatagcga
     4261 agaacgtcct attacaaaaa aacatattca ccaacagaat agaaagaaaa tacttttcag
     4321 agaagtagtt cagacgacta gacaagctta tgaagtatta aacaaattgt cgcgttatca
     4381 aaacgactta tatttactta ataacaattt cctattacaa atcaaattag atttagattc
     4441 attaactgct tttcacgagc aaattttagc tagcctttct aaaaaagcac gttataatgt
     4501 gacacatgtt gattatgagt tagataatcc gcagaaaaaa gatttattgt ctacatttca
     4561 gcatgagtta ataaatcatc cttatcaaac ggaatattcg tttgccaatg ttatgcaaat
     4621 tgttgcagcc atcgaagagt atcgacacca tttagagcat ttagaccgca taagaattag
     4681 ctttttcaca tatcatagat ctgatgcaga cattgaaatt gttgaagaag actttgattt
     4741 ataagtttaa agcaattcct gtaattaata ccacaatgag ataaagtaat attgaatctc
     4801 gaaattaaat ttaaaaaagc agtaagatta ttttcaatga agaaaatctc ttactgcttt
     4861 ttctatgtta tgaccccacc atcctgttac aaatcttccc tacaagattg tactgaagtc
     4921 aaacaactaa tactttataa gttttgaatg ctatataaat gctcgtaagc accttgtttt
     4981 gcaatcaatt cacgatgcgt acctgtttca acaatatgtc cattttcaat tacgacaatt
     5041 ttgtcagcat gtgtaatagt ggacaagcga tgcgctacga taagtgtcgt tcgatcttta
     5101 ctcaacacat ctaatgcttc ttgaataatg gattcacttt ctaaatcaag tgcacttgtt
     5161 gcttcatcca agataagaat tggcggatta tttaaaaata ttctagcaat cgataatctt
     5221 tgtttttgac cacctgataa tttaacacct cgttcaccta cttcagtgtc atatccctgt
     5281 ggcaagttca taataaagtc atgtgcatta gccattttcg ccgcttcaac tacttcttca
     5341 tctgttgctg ttggacgacc aagtaaaata ttttccttaa ctgtatcaga gaataaaata
     5401 ttatcctgtt gcaccaatcc tatttgattt cttaaacttc ccgttaaaaa atctttaatg
     5461 ttgtgaccat ctattaaaat ttgcccagaa gttacatcgt aaaatctcgg tattaagtta
     5521 attaatgttg atttaccacc accactcata cctacgaaag caactgtttc tcctttttca
     5581 atactcaaat taatatcttt taaaattgga gcttcgttat cgttatattg aaaactaaca
     5641 tgatcaatat caatacgacc ttgtttaatt tcaataggtt gagcaccaac accatttttg
     5701 atgtcataat cttcatcaat taattggaat acacggtcca ttgaagcaaa actttgcgtt
     5761 aaagttgtaa atgatgcgac taaacgacgt aaaggcccga acaataactc caagtatcca
     5821 acaaatgctg caagtgtacc tactgtgatt gatccagaaa tagcaagata tgcaccaaca
     5881 ccgatgacaa taattggtcc aatatctgta actgtattaa ttgcggcaaa ggaataggca
     5941 ttccatcttg tatgtttcaa cgcacgtgtt aagaaattag tattcttttt atcaaagttt
     6001 ttcgcttcat tgtcttcaat cgcaaaactt ttaacgactg aaataccttg aacacgttca
     6061 tgcaagaatc cttgaacctc agctaatgct tgagatcttt cacgtgtcaa ttttcttaat
     6121 cttccaaaga aaacgtacac cgttaaaatg taaaatggga agataaacag tgctgctaaa
     6181 gtcaatttca catctaaaaa gaacattatg gatagtgcaa taataattgt tatacaatct
     6241 aaccaaatat tcattaaccc ggttaaaatg aaatcttttg tttgttcaac atcattaatc
     6301 actctagata ttacttgacc tacttgatta ttagcataaa atctcgcact taaagcttgt
     6361 aaatggttgt ataacttttt acgtatatca tacaatattt tattacttgt ccattgcgcc
     6421 aaatattgac gtataaattc aattggtggt ctcactatta caaaaataaa taatgcgata
     6481 ccaatggcaa tagttaaatg atgaactttt tcatcagtcg ttagtgcgtg gttattaatc
     6541 acgccatcta ttgcatattt aattaatagt ggtataagca ttggtatacc aaacttaatt
     6601 atcccaacaa taaccgttgc aaaaatacga tatttgtatg gcttaacaaa ttgcaaatat
     6661 cgtttaatca tacaatcccc ccctaatcta ttgccctatc ctattcataa gcataaaaat
     6721 gaatagaggt tggatacata atttgtagat gtaaattctt cttacaattt acatttttaa
     6781 aattaagata tctcaacctc tgtcatattc atgtgattaa atacgttgtc ttattcgtat
     6841 gttatattct attcaattta atctatggat actgtgtccc cacacgacag caaaagttat
     6901 catacttctt tacatcacta agtcaatata aatgatttaa tcagtattta cactttattt
     6961 gcttaatact gtctaatttt tttgtaacgt tctttccaaa ctttgataaa atctggcgcg
     7021 aatgggccct tcttctgttc tatccattgt tgaagaatgt ccacgttgcg tcttaaaata
     7081 atatcaatat catgcggata attcatttga ttcatatgtt gctcatattc atcttcatct
     7141 aataaatgat actttccgtt tggatatact ttaatatcta aatcatagtc tatatatttt
     7201 aatgcctctt catcacaaac aaatggtgat gacaaattgc aatagtaata aattccatct
     7261 tctctaaaca tgcagataac attaaaccaa tattctgagt gaaagtaaac aattgccggt
     7321 tcacgtgtta tccaagttct tccgtcactt tcagttacta acgtatgatc attgccacca
     7381 atgacaacat gatcagtacc ctttaatatt gttgtttcag accaaacgcg atgaatctta
     7441 ccatcatgtt tataactctg aattttaatg ttttcccctt ctttaggtat ggattctctg
     7501 accatactcc acaccacctt ttgttaattt aaccattata aattatagca tatttcagaa
     7561 atagtattat ataaatacat atttttacga aataagattt tactacttaa taattaaact
     7621 cggtaatatt gctaagtact acaacagaga tttacatgtc ccatttaaag tatataaaat
     7681 catcactttt atatatcaac actttaactt tttgacattg ttattctatg agatttaaag
     7741 atatcattta tactttttaa aattaatgtc actatgtttt ccgataatat taccaatcat
     7801 cgaatgttac ccatttataa attgataaat ctttgacata ggtacaggga atgtatattg
     7861 atctcgatca cttaaatcaa accaaatcat gtcatctggt aatgtttcaa tgttaattgc
     7921 tcctgaaacg gcgtatactt taatcttcca tgttaaatga gtaaattgat gcttcaactc
     7981 aaaaataggt gtttctactg gttgaatgtc atgaccgatt ttttcagtca ttttacgtct
     8041 agcatgctca ctttcaaaca taggaaattg ccacatacca tgcaataatt tttcgctacg
     8101 cttttgcaac agatattgac cttgattatt tctaattaaa aagacggatt gctcaattac
     8161 ttttttactt acatttttag atttaacagg taacttttca aatgtacctt tatcaaatgc
     8221 ctcacagttt tcttgaactg gacaaaataa gcataatgga ttttttggtg tacaaattaa
     8281 cgcccctaat tccatcatag cttgattaaa cgttccagct tctgtagtaa catacggtaa
     8341 taattcttgt tcgtacgatt tcctcgtcga ttgtaattta atatctcgat agtcatcatt
     8401 caatctagac catactcgaa aaacatttcc gtctacagtt gctagtggta cattatatgc
     8461 aatgctcatt actgcagctt gtgtgtatgg gccaacacct tttaacgctt taaattgatc
     8521 aggatctttg ggaactaagc cttcatattt atcatgaact tctttaatcg ccgtatgaaa
     8581 atttcgagct ctactataat atcctaagcc ttcccaatac tttaacactt catcttccga
     8641 agcttgactc aaaacttcca cagttggaaa tcgttcaaca aaacgatgat aatagtcaat
     8701 aactgtttta acttgtgtct gttgtaacat gacctcactt aaccaaatat agtacggatt
     8761 ggtcgtttgt cgccatggca tttctctttg attttcatca aaccagtgta tcaaattttc
     8821 tttaaaacta gactgctgat acatttataa aaccctttcc tcaccaaaat taattgtctt
     8881 tactcataat gtttttattg tacattaaaa tcatggttag tatgtaagtt aatttagtta
     8941 tttgcgaaat tggattataa tagtatatat aatattatga aatgagtgaa ctgatatgga
     9001 cactgcaaca catatcgcaa ttggggtggg ccttacagca cttgcaactc aagatccagc
     9061 aatggcttct acgtttggtg caacagctac aacccttatc gttggttcat taattcctga
     9121 tggggatact gttcttaaat taaaggacaa tgcaacatat atttcgcatc atagaggtat
     9181 cacgcattcc atccctttca caatactatg gccaatttta attacatttt taatattcac
     9241 gttctttagt ggaaccaacc catttcatgt atggatgtgg gctcagctcg cagtattttt
     9301 acatgtcttt gtagatatat tcaattctta tggtacacaa gcgcttagac ctatcacaaa
     9361 caaatggatt caattaagtg tgattaacac atttgaccct attattttca cagttctttg
     9421 tattggtatt gtattatggg ttataggctt gcatccattt gcagtcttct ttcctataat
     9481 cgctttacta atcatttatt acatgattcg ttttaaaatg agagccgtaa ttaagcaaca
     9541 agctttaaaa gcaattcaac aagagcatca ccctgttaaa gtatttgttg cgccaacaat
     9601 aaaatttatg gaatggcgtg tcgcgataca aactgatgca catgactatg ttggaaaagc
     9661 atatggtagg aatgtggtgt ttagtgataa agtggaacgt caaacattat caacagactc
     9721 cattttatgg aaagtcaaag gtaataaaga tatacgtact tttttaaact tttcatcaat
     9781 ctatcgttgg caaacaacaa cgttagcaga tggttctact gaaattcgtt tgattgattt
     9841 gcgttattta aaaaatgatc attattcatt tgtggcaatt gcacatgtaa caaacgataa
     9901 tgtcatagac cactcttata ttggctgggt atttacagaa gataagttac aacgtaaact
     9961 gtatgctaaa taatttcaag ttattattca ctaaagttaa tctataaaaa atgaacaacc
    10021 gggcagaatg aaaatcaaaa cgatttttac tctgtccggt tttttaatgt aaaactatga
    10081 atgcttttac aaaatctaaa atttatattg ttgctaacaa actaccttta atgactcgaa
    10141 atatcaaaat cagtatagga aaacaatatc tagatgatat tctaattgtt tctgattctc
    10201 acagattaat ttacacaaca ggtcagctaa acatcatgaa gaagtatccg cctcgtctgt
    10261 actatcattt gaaacatcct gttgattatc agtttgtgtc actttagatg ttttattata
    10321 aattgcgtgt gtcgtatacc ttgctaatac gaaatttact gcagctatta aacataagaa
    10381 taaaataaag taaaaatgat atggaatgtt ttccatactt gatataccaa atatgataga
    10441 ttgtgctata ccattaacaa tgtaatacat tggatttagc attaggatgt gattgataaa
    10501 tacatgattt ggatttggta tgaaaataat tggtaacaat aagaaacaca atacacaaac
    10561 cccataaaat atgatattta ttttttcagt taacagtcga ataagaccaa aagtaacgga
    10621 tattaatcct acaaaaatag ttgccatcac aataaaatag aatagcgcta tatatgatgt
    10681 ttcgaagttt actggtttaa ccaatgcact aatcatcgtc aaaatgacta gcataataaa
    10741 acttaaaata gacataataa ctactggcgt cgctgcggaa atattaaata acttagtaac
    10801 taaaaagtca ttcatataat gatgataaga gatataaatt gtgaacatca ctaccgcaaa
    10861 aacaatcaat acaatgattc taaataacca tctcacctct ttaacatcgg tcaaatgact
    10921 aaaggaaaat agcgtttcac ttgcaataat aagtattaaa catgcagcta atacgattag
    10981 aaaacttttc caactttgtt ttaaacgttc tgttacatac cttttagtat gcggtaagtt
    11041 tttaaaatat aaaataatat tatctagcat ctttacacct acaattcaat ataaatccat
    11101 ttgtcttctt tcaaattggc aatacaatat ccattcccaa ctttagtaat ccaaatgtta
    11161 ttgttaatat taaacttatc ttttaattct tttttatcta caattggata aacaaaaccg
    11221 tataactgat tattatcatt gaaaagtaat tgtatcggtt gttgtgtaac ggatgcaacg
    11281 aaacggttat catcatcagg aactaaagtt tttataactg aactatgctt tttatgtaat
    11341 tgactattaa agtatgcata gtaattacta taatttgatt tcatatatgg tgctaatttt
    11401 ttaaaactat gcgtttgata taaacctgcc gggttaaaat attctaattt atctttacta
    11461 acatagtaag tcttattatc ggcggtaaca cgataatttt tactattttc gccagtaatt
    11521 gtaactacac tatattttgg aattgtgact tgtttatccc cttgcatatc cacactgcca
    11581 ttaaaagcaa ttccataagc taacttttcg ccatatggat ctttattttc attttgcatc
    11641 gtagcacgat tcgtaaaatc tgttatcgaa ataattccta tattattgaa aatgagcaac
    11701 atcatcaatg ctaagcctaa aatagtacct gaggctaaag tccaaaaacg cactaaaaac
    11761 ttaggcggtt ttgcatgatt atagcgctca acacgtttga aattataggt catctctggt
    11821 attcttgtac gattcttttt ccaatctaaa tcaaagtttt caatttcttc ttttgagttt
    11881 agacttaatc gatcacgttc atgttcttta aaagatggaa taacttgttt aagtgaccct
    11941 tccattctta attgaccatg tgaaaaccaa gctatgtagt tactcacttg tgaaatttta
    12001 tcaatatcat caccaattga cacaatcgtt aaattatttt caatataatc atttgttaat
    12061 tcaatcgcac gttccataaa ttgtggtgtt aaatagtcaa taacatgatt taaaataata
    12121 atatttgatt ttgatgagcg tgcaatactt aatagtaatt gagcgtatgc cgctttcgaa
    12181 atatggttaa ccggcttcgt tttataatca cctaaatgtg catattgaat aatctgttca
    12241 gctttatgat cattaatttc atatggaaat agttgaacta actgcgctgt ataagcttca
    12301 acagtttgat gaattaacga ttgatcttca atatatccgt agaacaaatc ttcagtacaa
    12361 actactttac ctttatcagg tttaattgca cctgccaaca attgacctac caacgctttg
    12421 gaagattcag gttcaccaat tatacctaat gcttctcctt gataaatatg taaactaata
    12481 ttgtttaaat cgatatcttc agcatcatat ccaaaaggta aataccattt cttattctgt
    12541 ttattcctat agtagtgtgt tacttttagt aactttaaaa caattgaact tcccatctat
    12601 tttcatcctt ctataattgt aataacgcta taggtaagcc ttcttcgggt tcatcgctat
    12661 taaatctaaa cccccacgca aaaatacctt ttaatctttc tactttaaaa tattgattac
    12721 tgccatcagt cagtttataa attttaccaa tttctatctt ttttctgtct actaagtaac
    12781 tttcggcaac aattacttta ctttgataaa cgtcatattc atttaaaata ccattcatct
    12841 cagcttttct cattttctct ttataaagtt gtatttcgtg tcttaattct agctcagaca
    12901 tctcactcaa tttcttctgt tccatcggca ataccacttt cttctaattt agctttaatt
    12961 ttatcatatt tatatccttt tctcataagg ccttcgatag tttttgaaat taatttctgt
    13021 tgcgtgtact tctttcgatt tttattataa attttttcta aatctcgttg taataaatcg
    13081 tctaaaaccg cttcatcttg tgtaaaatcc atttcattca atacagcatg aatggtttcc
    13141 atttcaaacc ctttttgaat taaagattgc attacttttt ctttaacttt attttgtggc
    13201 cctttttttg ttttagatat tttttcagca atttggataa tatcatcaag ttcctgttgt
    13261 tctctataaa gttctgtaaa tatttcaatg atatttggtt ctataccaag ttgatatagt
    13321 ttttgttgat aaattttagg tcctttatct gtcgtgcgaa tcattgtatt ttttaaactt
    13381 tccgcataat cttgatggtc gattaacttt tcgcgataac aatattcaat cacttcagaa
    13441 atcgcttgct ctgatatctc ttctttttgt aaatattgta taacttcttt ttcagttctc
    13501 tttttatatg ataaatattg gattgcttta tttaaaccta tgcgataatg atcatacttt
    13561 tgaatctctg ccatgtcagc agcttcaagt tgttgccctt tttttaaatt aaatttgact
    13621 aatgtatcaa tatctatacc catttcaaat tgttcgtcta aaaaaagatt aaaccgttct
    13681 ttgtttttct tttgaacttc tattttagta atcttcggca ctggatcacc ccgttacttt
    13741 cttaagtttt aatacaatac tcttttatta ttcaataagc cacttcctat agcaaatgtt
    13801 taactttaaa tatttttcga tgctaacaaa aaatcacact atcatctttt aaaatgaaag
    13861 tgtgattaca agcaaatctg taaaatttat aaagcagaaa caattcaact ttatcattat
    13921 gacatttcaa ttaaaccttc tacattatag ttccaagcat cttacacatg aatgcaagta
    13981 tttaacgatt taattgtgac atagcctgtt gatattgtgt ttcattgata taattttgtt
    14041 gcttcatttt ttctaagttc gtgcttacac gttgcgtaaa attctctgac atattattga
    14101 tattatatac gctaggtgca ttgactttac tagctaaaat agcgctttgt aaaactgtta
    14161 tgtgagacat tgttgtacta tttttattca cggttgttcc aaagtaatgg tttgctgcgc
    14221 cctcaagcgt atattgatta tccccaaagt aaatattatt taaataa
//
LOCUS       DAHHYJ010000066        14252 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000066 DAHHYJ010000000
VERSION     DAHHYJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.066"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            147..1367
                     /locus_tag="NBK22_001476"
     CDS             147..1367
                     /locus_tag="NBK22_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCD2414665.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKEYEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            1364..1852
                     /locus_tag="NBK22_001477"
     CDS             1364..1852
                     /locus_tag="NBK22_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCD2414666.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(1951..2640)
                     /locus_tag="NBK22_001478"
     CDS             complement(1951..2640)
                     /locus_tag="NBK22_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCD2414667.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKNGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVIPIGLRIER
                     NISKKHSHTFL"
     gene            2767..3210
                     /locus_tag="NBK22_001479"
     CDS             2767..3210
                     /locus_tag="NBK22_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2414668.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNIELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKNNLPMISLNKDLGYQVSHVKMYKDID"
     gene            3277..3666
                     /locus_tag="NBK22_001480"
     CDS             3277..3666
                     /locus_tag="NBK22_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414669.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKEASQDKQSKDQ
                     TKSSDKDNHKKPTSTDKDQKANDKHQS"
     gene            3804..4103
                     /locus_tag="NBK22_001481"
     CDS             3804..4103
                     /locus_tag="NBK22_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414670.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGRTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            4183..4725
                     /locus_tag="NBK22_001482"
     CDS             4183..4725
                     /locus_tag="NBK22_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2414671.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKETHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKATLKLAEQHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(4821..5387)
                     /locus_tag="NBK22_001483"
     CDS             complement(4821..5387)
                     /locus_tag="NBK22_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCD2414672.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(5389..5847)
                     /locus_tag="NBK22_001484"
     CDS             complement(5389..5847)
                     /locus_tag="NBK22_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCD2414673.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(5850..6533)
                     /locus_tag="NBK22_001485"
     CDS             complement(5850..6533)
                     /locus_tag="NBK22_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414674.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRIKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(6705..7580)
                     /locus_tag="NBK22_001486"
     CDS             complement(6705..7580)
                     /locus_tag="NBK22_001486"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCD2414675.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGDNDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QISYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
     gene            7799..9430
                     /locus_tag="NBK22_001487"
     CDS             7799..9430
                     /locus_tag="NBK22_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCD2414676.1"
                     /translation="MKKLTTILFQYKIFPVLMFLVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLKYEENQKPFIQLTDISFRYDN
                     SDRLVLNDLNLEIYNGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTKQRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTKGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            9427..11100
                     /locus_tag="NBK22_001488"
     CDS             9427..11100
                     /locus_tag="NBK22_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCD2414677.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISIGHALLIMVS
                     MLFTLLVVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKNRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDERDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTASTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(11227..11670)
                     /gene="mgrA"
                     /locus_tag="NBK22_001489"
     CDS             complement(11227..11670)
                     /gene="mgrA"
                     /locus_tag="NBK22_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCD2414678.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            11897..12823
                     /locus_tag="NBK22_001490"
     CDS             11897..12823
                     /locus_tag="NBK22_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD2414679.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHH
                     GIKSMTYTFTGPIDRQLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            12926..13834
                     /locus_tag="NBK22_001491"
     CDS             12926..13834
                     /locus_tag="NBK22_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCD2414680.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHSIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            13869..14156
                     /locus_tag="NBK22_001492"
     CDS             13869..14156
                     /locus_tag="NBK22_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414681.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYGINHF"
BASE COUNT         4827 a         2041 c         2434 g         4950 t
ORIGIN      
        1 acagttggtt gctaatgcac caacagcaaa agagccccta attaataaat taaaagggga
       61 ctaaaggatt acagttggtt gctaacgcac caactgcata agagccccta attaataaat
      121 taaaaggggc tctaagaagc ggggttatgg atatgtttgc agcgttatta caaataaaga
      181 attataaact ctttgttgct aatatgtttc tactaggtat gggtattgcg gttacggtcc
      241 catatcttgt tctttttgca actaaagatt taggtatgac aacaaatcag tatggattac
      301 ttctagcatc tgcagcgatt agccagttta cagtaaattc aattattgct agattttcgg
      361 atacgcatca ctttaataga aaaattatta ttattctcgc attattaatg ggtgcgcttg
      421 gtttttcaat atactttttt gtagatacaa tctggttatt catattacta tatgcgattt
      481 tccaaggatt atttgcacca gcaatgcccc aactttacgc atctgctaga gaatctatca
      541 atgtttcaag ctctaaagat agagctcaat ttgccaacac agtattacgt tcaatgttct
      601 cattgggctt tttatttggt ccatttattg gtgcccaatt aatcggatta aaaggctatg
      661 ctggattgtt tggtggaaca ataagtatca ttttatttac tttagtactt caagtgtttt
      721 tctataagga tttaaacatt aaacacccta ttagtacgca acaacatgtt gaaaaaattg
      781 ctcctaatat gtttaaagac aaaacacttt tattaccatt tattgcattt attttattac
      841 acattggaca atggatgtat acgatgaata tgcctttatt tgttactgat tatttaaaag
      901 aatatgagca acatgtcggt tatttagcta gtttatgtgc tggtttagaa gtgccattta
      961 tgatcattct tggcgtttta tcatctagat tacagactcg aacattgttg atttatggag
     1021 cgatttttgg tggtttattc tacttcagca ttggggtatt taaaaacttc tatatgatgt
     1081 tagcaggaca ggtgttttta gctattttct tagcggttct tttaggaatt ggtattagtt
     1141 atttccaaga tatcttacca gattttccag gatacgcctc aacactattt tctaatgcaa
     1201 tggttattgg acagttaggc ggtaacctat taggtggtgc tatgagtcac tgggtaggtt
     1261 tggaaaatgt attttttgta tcagcagcat caatcatgtt aggtatgata cttatattct
     1321 ttactaaaaa tcaaaaaatt acaaaagagg atgtgatatc aacatgacaa ttattttatg
     1381 gctacttatc atcgctgcct tcatgttagc atttgttggg ttgattaagc cgattattcc
     1441 ttctgtttta gtattatggg ttggctttct aatctatcaa tttggctttc ataatcagca
     1501 tttatcatgg gtgttttatg tatctatggc attgctaaca atactaattt tatgtgcgga
     1561 ctttttagct aataaatatt ttgtgaatcg cttcggtggt tctaagtttg gagagtatgc
     1621 agctttaatt ggtgtggtta ttggatgttt tgttttaccg ccatttggaa ttattattat
     1681 accttttatt ttggtattca tagttgaatt aatacaaggc tattcatttg aaagagcagt
     1741 taaagtaagt ataggttcaa tcgtagcatt tttaacaagt agtatagctc aagcaatcat
     1801 tatgtttata atgattgtat ggttctttat agatgcttta ttgattaatt aataaaaagc
     1861 ttattgcaaa atatgttttt cggtaactgt aatttagtga ttttatcatt aacagtacca
     1921 aattcgcata tgatgtaata agctttttgt ttataaaaat gtatgagaat gttttttcga
     1981 aatatttctt tcaatgcgta atccaatagg tataactata aatgaaaata taataaatac
     2041 ccaattgctt acctgaatat atggtccaaa ggcaagtaat gattgtggaa taaagaatac
     2101 atagaaaaat cctacaatta gtagaatgat tgctaaatag gtgaatatcc atatccaatt
     2161 tgaattgtta aaacattgca gttgtattgt tttaaaacta aaccatataa ataaaaagac
     2221 aataaagaat ccgacgacta ctgaaaacgg gaacgaaaca aaatataaat tacttccatt
     2281 tttttccatg aaaaatccta aaaatccttt gagaaaacta acaatcccaa ttaatagaac
     2341 gatgtgttga tagatatatt taaaaatatt tttaaatgtt tcattaggca tcgcttttag
     2401 ttcttttatt gcatgtgctt ttgggtcgtg attgaaaaaa tctaaggcta ataaaccatg
     2461 ttgttctgcg cttaataatt gtttaagtat acggttaata attaactctg tatcatgagg
     2521 attgacgcga aagtcagagc gcatataagt catataattc tcgaagattt ctctatcagt
     2581 attgcttaat cttaatgatt taacattatt ttcttttgtt aattgcgcag tacttttcat
     2641 tgttacttaa gcgctccttt aaaaatgttt aattccaaat taaaatggaa atgattttat
     2701 agtattaata aggtcaatca tatcatatta aacgcataaa tataacgatt aatattggag
     2761 aggaaaatga ggacacttaa taaagatgaa cataattata tcaagcaaat agctaatata
     2821 catgagacat tattgtcgca agtagaatcc aactataaat gtactaaact gagtattgct
     2881 cttaggtacg agatgatatg ttcaagatta gaacatacaa atgataaaat ttatatatat
     2941 gaaaatgaag gtcaattaat agcgtttatt tggggacatt ttagtaatga aaaaagtatg
     3001 gttaacattg aactgctata tgttgaacca caatttcgca aactgggaat agctacgcaa
     3061 ctgaagattg cgcttgaaaa atgggcaaaa actatgaatg caaagcgaat aagcagtaca
     3121 attcataaaa ataatttgcc aatgatatct ttgaataaag atttaggtta tcaagtgagt
     3181 catgtgaaaa tgtataaaga tattgattag aattaggatt atgttgctaa ttcatgttaa
     3241 aattaaaaaa gatttaatga cgttaaggag ttttatatga agaaattaat catcagtatt
     3301 atggcggtca tgctattttt aacaggttgt ggtaaaagtc aagagaaagc cactctggaa
     3361 aaggatatcg ataatttaca aaaagaaaat aaagaattaa aagacaaaaa agaaaagctt
     3421 caacaagaaa aagaaaaatt agcagataag caaaaagacc ttgaaaaaga agtgaaagat
     3481 ttaaaacctt caaaagaaga taacaaggat gataaaaaag acgaagacaa aaataaagac
     3541 aaagataaag aggcatcaca agataagcaa tcaaaagatc aaactaagtc atcggataaa
     3601 gataatcaca aaaagcctac atcaacagat aaagatcaaa aagctaatga caaacaccaa
     3661 tcataattga attgcttact tgttatagat gaaaggtaca gcgttttaaa ccttatttta
     3721 agggtatgta ttaattaaaa tgtggtcatg attgaaacag aacgtaaaaa tagacaacgt
     3781 aattaataaa ggagagaaac ggcatgcatg aacaagattt tagaatttta gagggtcaag
     3841 atattacttt gccagaattg ggtagagaat tagaaaatat tacagggcgc acgattgctg
     3901 attctactgg cgaaattaag cgtgtaattg cacatttacc aaactttgag tccgatactg
     3961 atacttttgt tgctacatat cgtttaaacc atcaacaaga ttttatagat gcaactttta
     4021 ctgcgctgaa atcagataga gcacgtttaa aagaagtgcc agttcatgtt gaacttataa
     4081 gttatatttc taaatcaaaa taaactgcta tctaaaacgc aaagttgatc aaaatatcga
     4141 ttttgtgttt tttattgaga aattatatag gagtgtcaat cgatgattta ttgtgaaaca
     4201 gagcgtttaa tattaagaga ctggcatgaa gatgatctgt taccttttca aaaaatgaat
     4261 gcgaattatg acgtacgtaa atattttcca agtttattga gttatcgtcg ttcagaatta
     4321 gatatgagaa ctatggatgc ggttattaaa gattatggca ttggattatt tgctgtagaa
     4381 gataaagaga cacatcaatg gataggcttt ataggtttga attatattcc agaaacaagc
     4441 gattatccat ttaaagaatt accgctttat gaaataggtt ggcgcttgtt gccagaattt
     4501 tggggaaaag gattagcaac tgaaggcgca aaggcaacgt tgaagttagc agaacaacat
     4561 caaatatacg atgtctatag ttttacagca gaagcaaata aagcttcaca acgtgtaatg
     4621 gaaaaaattg gcatgacagt gtatgatcat ttcgaattac ccaatctaag taagtatcat
     4681 ttattaaaaa ggcaagtgcg ctattacatt aatcttcgaa agtgaaaaat ttatacataa
     4741 gcgtaacaaa cacccctaac attgtttagc tgatgataac aatattaggg gtgtttgttt
     4801 attctttaac cttagaatga ttaatcgtat gaacgagtac ccagaggttt gaaatttaat
     4861 attgattcta ttaatgattc cttagtgtcg cataacggtg caagagcacg atacttagga
     4921 tcaataaaac cttcctcaat catatggtca atcattgttt gtagtggatt gaaaaagcca
     4981 ttaatattat aaatggcaat aggcttttca tggataccta tttgagccca actatacatt
     5041 tcgaaaaatt cttctagtga acctgcgcca ccaggagcca tgacaaatgc atctgcaagt
     5101 tctgccattt tatttttacg ttcatgcata gaatcaacta aaattaattc agttaaacgt
     5161 tggcttgtga tttcatgttc atctaacatt ttaggcatga cgccaatagc tttgccgcca
     5221 tgatctaata caccatcttg aatggcaccc ataatgccaa ttgaccctgc accaaatact
     5281 aattcataac cttgttcagc aaaatattta cctaaatcgt atgctttttg tacatatgaa
     5341 gggtcatgac ctttgcttgc accacaataa actgcgattc gtttcatgtt aatccagctc
     5401 cttaattcga tgaatgactt ttaatagtga ttgttcaaac actttttgat cttgctttgt
     5461 aaaaggtggg ggacctttgt ggcgaccacc ttgttttcta atttgtgcat tcatatatcg
     5521 tttatctaat agttgttgaa tatttttgga attgtatatc ttcccattat gatgcatgac
     5581 aattaagact ttgtcgacta ataaacttgc gagtccataa tcttgagtga ctacgatatc
     5641 atccttcgtt gataattgaa caattttgta atcaactgca tctggtccat catcaacata
     5701 taatgttgat acatgtggag gatataattg gttcgaaaaa tggctgaagc tccgaataat
     5761 tgtcacaaaa atgcctgtct cagttgttaa atctataata gaatcaacaa caggacaagc
     5821 atctccatca ataataatat gtgtcacaat tatgcctctg tattgttttc tttattttgt
     5881 tgagaggcgc ttttggcaac ataatcttta tattttttaa atgacttgat gcgtgcttta
     5941 tcagcttctt gttggcgttt ttgttcttct ttgtgtcgtt tttcaatatt tttttgtaac
     6001 tttttattca ttttagcgat ttctttgcga tttttttcag ctagtttatc gccttttttc
     6061 tcagttttct catctaattt attaggtgtt aagcctgctt tttcttcgta tttttgtgat
     6121 tttttcatat ctttaatacg ttgtatttca ttcttttcgc gggctttttg ctcttcttta
     6181 tgacgctttt cgatattttt ttgaagtatt ttattcattt tatcagcgtc tttacgattt
     6241 tgtttagcta atttttcgcc ttttttctca atataggcag gatcatgttc tctagcaaac
     6301 tttttaagtt cacgtttatt ttcaaaatct tgttttttat cgccgacata ttctttaaca
     6361 tcactcgctg tgttactgat tgctgcagat gtttttgaag caactttact tgtagcatct
     6421 gtaacttttt gtacgtccgg atgttgtttg atacgtttac gttcaacaat taacggtacc
     6481 aatacaattg gtaatacatt aatcataaat ttgatgactt ttttcttatc catagatctt
     6541 gcctccataa ttactttatt aattttacat accctatgat acatcaatat aaacgatgat
     6601 agtagtgaat cactattaag tatttcagat gttttttaaa agaagacaat aaaaactgcc
     6661 aatcaagtga ttccttaatt gacagtctat attttaaaag gaaattaaat acctttacca
     6721 atgccaaatc cgaagtaaag tatagcaata aagattacta atacaattct gtaaatggca
     6781 aatggaatta gtttgatttt gttaattaga tgcaagaatg ttttgattgc aattagtcca
     6841 acagtaaatg cagctaaaaa gcctaaaata taaaaaggta tatcagcaat ctgaatatct
     6901 tgataatgtt ttaataaaga taaaccacta gctgctaaca taattggaac agccataata
     6961 aatgtaaagt ccgatgctgc tttatgattt aatttcatta ataccccagt tgaaattgtt
     7021 gagcctgaac ggctgaaacc aggccacata gctactgctt gagaaatacc aattacaaat
     7081 gcttggaaat aactgatttg atctactgtt tgtgggtttt taactttagc tgaatactta
     7141 tcagcaataa tcatatagat agcacctacg aataagccaa tcataacagt tggcacacta
     7201 aataaatgtt cttcgatgaa atcatcaaat agtaagccta aaatacctgc tggcaccata
     7261 cccactaata catgtaataa atttaaacgt cttggctttg aacgtctttg ttgatcgtta
     7321 tctccttcaa catgtttgtg tttaccaata tgtaaaatct ctaagaagcg ttcgcggaac
     7381 acccatgctg ctgcaaagac ggatcctaat tggatgacga ttttaaatgt aaatgctgac
     7441 tgagaaccta aaaattcaga tgattttaac cacatatcat caactaggat catatgtcca
     7501 gtagaggaaa caggtgcaaa ttctgttaat ccttcgacga cccctaagat aatacctttt
     7561 attaattcaa tgataaacat aatgtaccca ctttcattac tcaatttaat ttatttaaat
     7621 atcaaaatta ccatatcatg atagcatatt catttaaaga catgctagtt atagttataa
     7681 tactagacta aagatgtata tattcatttt cttttacatg taaaactaca atattttatt
     7741 gagctattta atttgatttt aaggaaaacc ttttataata ggtttaggtg atataattgt
     7801 gaaaaaatta acaacaatac tgtttcaata taaaattttt ccggtactca tgttcttggt
     7861 cagtactggt ctcggcatac tcgttataac gcaaaatatt ttaatagcag attttttagc
     7921 taaaattata agacatcaat ttcaaggttt atggattgta ttatttattt tattaggtgt
     7981 tttactttta agagcaactg tgcaatttct aaatcaatgg ttaggtgata cattagcatt
     8041 taaagttaag catatgctta gacagcgggt tatttataaa aataatggtc atccaatcgg
     8101 tgaacaaatg actatactca cagaaaacat tgatggttta gcaccttttt ataagagtta
     8161 tttgcctcaa gtgttcaaat caatgatggt tccgctcatc ataatcattg caatgttttt
     8221 catccatttc aataccgcat taattatgtt aataactgca ccatttattc ctttgtttta
     8281 tattattttc ggtttgaaaa cgcgagatga gtcaaaagat caaatgactt atttgaatca
     8341 atttagtcaa cggtttttaa atattgctaa aggtttagtg acgttaaagc tatttaatcg
     8401 tacagagcaa acagagaagc atatttacga cgatagtact cagtttagaa ctttaacaat
     8461 gcgcatttta cgcagtgctt ttttatcggg attaatgctc gaatttataa gtatgttagg
     8521 tattggattg gttgcattgg aagcaacgct aagcttagta gtatttcata atattgattt
     8581 taaaactgcg gcaattgcga ttattttagc gcctgaattt tataatgcaa ttaaggactt
     8641 agggcaagcg ttccatactg gaaaacaaag tgaaggtgcc agtgacgttg tgtttgagtt
     8701 tttggaacaa ccgaacaata ataatgaatt tctattaaag tatgaggaaa atcaaaagcc
     8761 atttattcag ttaacagaca tatcatttcg atatgataat tctgatagat tggtattaaa
     8821 tgatttaaat ttggaaatat ataatggtga tcaaattgca cttgtaggtc caagcggggc
     8881 aggtaaatcc actttgacac atctaattgc aggtgtttat cagccaacaa taggtactat
     8941 aagtacaaag cagcgtgatt taaatatagg aatacttagt caacagccat atattttcag
     9001 tgcttctata aaagagaata ttacgatgtt taaagatata gaaaataata ctattgaaga
     9061 agtgttagac gaagtaggtt tattagacaa agtgcaatct ttcacaaaag gcattaacac
     9121 aataataggt gaaggaggcg aaatgttatc tggtggacag atgagacgca tagaactttg
     9181 ccgtctttta gttatgaagc cagatctcgt tatatttgat gagcctgcaa ctggtttaga
     9241 tattcaaaca gaacacatga ttcagaacgt tctgtttcaa cattttaaag atacaacgat
     9301 gattgtcatt gcacatagag ataatacaat tcgccattta caacgacgct tgtatataga
     9361 aaatggaaga ctgattgctg atgatcgcaa tatttcagta aatataacag aaaatggtga
     9421 tgacttatga aaacacgact aaaatttcaa gtagataagg atttattgtt agctatagtt
     9481 gttggtgttt gtggaagttt agttgcgctc gccatgtttt tcttaagtgg ttatatggtg
     9541 acacaaagtg cacttggtgc gccactatac gctctgatga ttttagtcgt tacagtaaaa
     9601 ttgtttgggt ttttaagagc tattactcga tacgtagagc gccttatttc tcataaagct
     9661 acatttacaa tgctacgtga tattcgggta cagtttttcg gtaaattagt aaatgtcatt
     9721 cctaatgttt accgtaaact gagttctagt gatttaattt cacgtatgat tagtcgtgtt
     9781 gaggcattac aaaatatata tttacgtgtt tattatccac cagtcgtcat cggtttgaca
     9841 gcgctagtta cagtcatagt tttggcgttc atttcaatcg gccatgcgct attgattatg
     9901 gttagcatgt tgtttacttt actcgttgtt ccttggttaa gctcaaaaaa agcacgtact
     9961 ttaaagaaac atgcagctaa tgaacaggcc cgatttttaa atcattttta tgattataaa
    10021 gctggtatgg atgaactacg tcgatttaat caaattaatc attatcgaga taatttgatg
    10081 gctaaattaa atcattttga taaattacaa cttaaagagc aacgcttttt aacgatttat
    10141 gattttatat taaatattat tgctatgctt tcgatttttg gtagtttagt tctaggatta
    10201 attcaaatta atgcaggcca actaaatatt atttatatga cgagtatagt tttaatggtc
    10261 ttaactttat ttgaacaagc tgtaccaatg acaaatgtcg cgtattataa agcggatact
    10321 gaccaagcat tgcatgatat taatgaagtg atatctgtac cttctactaa tggaaaaaac
    10381 cgtcttaatg ataagtatga tgcaacgaac atttatgaag ttaaggacgc tagttttaag
    10441 tattggaacc agcaaacgta tgtgttgtcg gatattaatt ttaatgttaa tagaggcgaa
    10501 aagattgcga ttgtggggcc ttctggttca ggaaaaagta cattactaca aattatggct
    10561 gggttatatc aattagatag tggctctatt cgtttcgaaa atatggatat gtttgaaata
    10621 gatgacaaag ataagtttga atcgttaaat gtcttgctac aatctcaaca attatttgat
    10681 ggtacaatac gtcaaaattt atttaccgat gaaagagatg aagcagtgca agcaatattt
    10741 aagcaattag atttagaaca tttggcacta gaacgtcaaa ttgacttaga tggtcataca
    10801 ttatctggcg gagaaattca gcgtttagcg attgcgagga tgttattaaa agatactgca
    10861 tcaacatgga ttttagatga accaacaact gcattagata aacaaaatag tttaaaagtt
    10921 atggatttaa ttgaagcaca tgcagaaaca ttaattgttg ctacacacga tttaacttta
    10981 ttgtcacgtt ttgagaccat cattgtgatg ataaatggta aaatagttga aaagggaaac
    11041 tatcaacaat tactcgctaa tcaaggtgct ttatggaata tgattcaata taatgcataa
    11101 aaaaactgct tgataggcta agaaaccttt caagcagttt tttaactaac aagcttacgc
    11161 gtgctaattg tttttctatt cttaataaat tctaaacatt actttaattg tcatgacaaa
    11221 agttaattat ttttcctttg tttcatcaaa tgcatgaatg actttaccta ataagcgatt
    11281 aagttcttta acttcatctt gagataaaga agaagctgaa gcgactttgt cagatgcatt
    11341 acttaattct ggtctaatag tttcactttt gtcagtcaag tgaataaata cttcacgttg
    11401 atcgacttcg gaacgttcac gcttaattaa gtctacttgt tccattcgtt ttaataatgg
    11461 tgatactgta ccagtatcga gtgctaattc agttacgact ttcttgacgt ttacaggaga
    11521 ttcatcccat aaaattgtta agacaagaaa ttgtgggtat gttagattgt acttcttaaa
    11581 aactttgtta gagtagtagc gattaacttg tctttgagca ttgtacaaac taaagcatag
    11641 ctgttctttt aaattatgtt gatcagacat taaagttctc ctccagacat actatccgtt
    11701 tttttctctt ttcggattgg taatcattaa aaagttgatt gtttattaat tcacaacttt
    11761 ctttgattca atgccatgct aaaattaaag tatgtttaaa gtttagaaga tatttttgat
    11821 taaatcaagc aaaaagataa tttaatatat atgttatcat ttttaaaaat aactgtaata
    11881 gaaaagagaa tataaaatga aaaataataa agatgaaaaa ataagaatat ccataattaa
    11941 cggatttttg ggtagtggta aaaccacgtt actgacacat tatattagtg aattattaaa
    12001 aaatgatgag aaaattaaaa tcatcatgaa tgaattcggt acttttgata ttgatagcaa
    12061 tagtatttca aatgaaattg aagtccattc attgattaat ggttgtgttt gttgcgatct
    12121 taaacaagaa cttgtctatg aactaaaagc cattgcttta aaaggggacg ttaatcatgt
    12181 catcatagaa gcgacaggca ttgcgcatcc tttggaatta ctagttgcat gtcaagatcc
    12241 gcaaatcgtt aatttctttg aaaagccgat tatttatggt gtattagatg cgactcgatt
    12301 tttagaacgt catcaatata ccgaaaatac agtttcgctg atggaagatc agttgaaact
    12361 aagtgacatg attattatta ataaaattga tcttataact gatgacagtc ttgagaaaat
    12421 tgataagcaa ttaggtatga tttgtgcaag tattccaact tataaaacaa cctatggaaa
    12481 agtttcgttg gaagaattgg acttaactgt taaagacaga gagatatcgt ctcatcatca
    12541 ccatcatcat gggattaaaa gtatgactta cacgtttaca ggtccgattg atcgtcaatt
    12601 gttttatcaa tttataatga aattaccgga atctgttcta cgtttgaaag gttatgtgtc
    12661 atttagagat caaccaaatg caatttatga atttcaatat gcatatggtt taccagacta
    12721 tggaataatt ggcatgcaat taccattaac gattgttatt attggtgaaa ctttagatac
    12781 aaatcacata cgtaatcaat tggatatgct acaatttacg taaatcagaa aatgtgttgt
    12841 ttcaatttaa cggaaatagc acattaattt cttaatactt ttattgtgaa tattttgtga
    12901 cattgaggga tggagtggat gagatatgga acaaataatg attaatcact atgttcattt
    12961 ttctaggctt gtacaaggtt tttggcgtgc aaatgaatgg aagatgactg cgaaagagtt
    13021 aaattatttt ataaatgaat tagttgaacg tggaattaca acgatggatc atgctgatat
    13081 ttatggggat tatcaatgtg aatcactgtt tggtaatgct ttggatttat cacccgaatt
    13141 aagaaataaa attcaaattg ttacgaaatg tggtatcatt ttgccttcta agcaatttga
    13201 ttttacaaat ggacatcgtt atgatttgag tagtaagcac atcgtgaaat ctgttgaaca
    13261 gtcattaatc aatttgaatg tagattattt agatagtcta ctcattcatc gtccttcacc
    13321 attgatggat ccagaacaag ttgctgatgc attaactaaa cttgttaaac aaggtaagtt
    13381 gaagtcattc ggggtgtcga attttaatca ttcacaatac caattgttaa atcaatatat
    13441 tatgaaagaa agactacata ttagcatcaa tcaattagaa ttatcgccat atcacgttga
    13501 tagtttacaa gatggaacaa tggattcaat gtatcaaaac catgttcaaa ttatggcttg
    13561 gagtcctttt gcaggcggta aaattttcga caaggaagat attaaagcgc aacgtattat
    13621 gaaagttgtt caatcaatag ctgacaaata tggtgtgagt gacacagctg tgatgatagc
    13681 atggttagta aaaataccgc atagtatcat gccgatactt ggaacaagtc agttaaagcg
    13741 tattgatcaa gcaatcgaag ggctacaact taatttagat gatcagtcgt ggtttgacat
    13801 ttacaccgct attatcggac aagatattcc gtaaacttat ttacttttaa atcataaagg
    13861 agcatatcat gacaaacgaa gataaacgtt tcgaacaatt aagatttgaa cgcaaattta
    13921 tagttattcc gtatttaatt tatgcagtca ttgtattact attaaatatt ttctattcgg
    13981 atttgaaaat aacaatgaca ttattcggac ttttctttgc gtataatgta gtcattttgt
    14041 tcatagcatt tattaaacat tataaacgta cattgttact aagtcttata ttaacagtgc
    14101 ttagtggcgc ggcattcttt ggaattattt atgtttatgg cattaatcat ttttaaatat
    14161 taaaaagaga ctatctacaa taatctcatt tgcattttat ctaactaagt ttaacttttg
    14221 cttagaaaaa tgatgctatg aggatattca gt
//
LOCUS       DAHHYJ010000067        14180 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000067 DAHHYJ010000000
VERSION     DAHHYJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.067"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(73..402)
                     /gene="ecb"
                     /locus_tag="NBK22_001493"
     CDS             complement(73..402)
                     /gene="ecb"
                     /locus_tag="NBK22_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCD2414682.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNAETVKKYNEYQ
                     TNFKKQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
     gene            complement(596..763)
                     /locus_tag="NBK22_001494"
     CDS             complement(596..763)
                     /locus_tag="NBK22_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414683.1"
                     /translation="MKRIVILENVIVDDPIRLNHVDYLVKSVLLKSIKLRFQEVRKFE
                     ILVKIMNRIHI"
     gene            complement(891..1394)
                     /locus_tag="NBK22_001495"
     CDS             complement(891..1394)
                     /locus_tag="NBK22_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD2414684.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKYNDVKAFYTHGHLYQVNRTRDLLAEKGLELGCLF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVIIDDQTLHGTINFKNRHHE
                     TISHTTF"
     gene            complement(1387..1974)
                     /locus_tag="NBK22_001496"
     CDS             complement(1387..1974)
                     /locus_tag="NBK22_001496"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCD2414685.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGENGFGYDPIFYVPKL
                     DKTMAQLSKEQKGQISHRRNAINLLQAFLEGEKNV"
     gene            complement(1986..2786)
                     /gene="racE"
                     /locus_tag="NBK22_001497"
     CDS             complement(1986..2786)
                     /gene="racE"
                     /locus_tag="NBK22_001497"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCD2414686.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(3022..3837)
                     /gene="sdhB"
                     /locus_tag="NBK22_001498"
     CDS             complement(3022..3837)
                     /gene="sdhB"
                     /locus_tag="NBK22_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCD2414687.1"
                     /translation="MTEQSVKNTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(3837..5603)
                     /gene="sdhA"
                     /locus_tag="NBK22_001499"
     CDS             complement(3837..5603)
                     /gene="sdhA"
                     /locus_tag="NBK22_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCD2414688.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYISNIDRSYTDMDESIFEKRKAEEQERFDKLLAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYEDIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(5655..6269)
                     /locus_tag="NBK22_001500"
     CDS             complement(5655..6269)
                     /locus_tag="NBK22_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCD2414689.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(6593..8374)
                     /gene="uvrC"
                     /locus_tag="NBK22_001501"
     CDS             complement(6593..8374)
                     /gene="uvrC"
                     /locus_tag="NBK22_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCD2414690.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRRFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLQVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIDGIGNKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            8540..8635
                     /locus_tag="NBK22_001502"
     CDS             8540..8635
                     /locus_tag="NBK22_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414691.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(8698..9012)
                     /gene="trxA"
                     /locus_tag="NBK22_001503"
     CDS             complement(8698..9012)
                     /gene="trxA"
                     /locus_tag="NBK22_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCD2414692.1"
                     /translation="MAIVKVTDADFDSKVESGVQLIDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(9185..11533)
                     /locus_tag="NBK22_001504"
     CDS             complement(9185..11533)
                     /locus_tag="NBK22_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCD2414693.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFHQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(11543..13255)
                     /gene="polX"
                     /locus_tag="NBK22_001505"
     CDS             complement(11543..13255)
                     /gene="polX"
                     /locus_tag="NBK22_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCD2414694.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTELKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITIDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLRVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILPDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHLAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLNADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNI
                     KLKK"
     gene            complement(13328..13849)
                     /locus_tag="NBK22_001506"
     CDS             complement(13328..13849)
                     /locus_tag="NBK22_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCD2414695.1"
                     /translation="MVIDFIIIIFFVYFVMVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KAIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLCLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(13850..14116)
                     /gene="zapA"
                     /locus_tag="NBK22_001507"
     CDS             complement(13850..14116)
                     /gene="zapA"
                     /locus_tag="NBK22_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCD2414696.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
BASE COUNT         4321 a         2709 c         1951 g         5199 t
ORIGIN      
        1 acacaatatt ataaaataat tatacctttg gatatagcaa aaagccacac tctatagcat
       61 ggcttctatc aattatttta aagtattata ttttaaaact agatcgattt gtctttgtaa
      121 ttttttcttt tcataactgt gttggaaatg aattaagtta acagctcttt gtgctttacg
      181 gtgtgttgca acagttctag tacgtttgaa caagtttaca gctttttgtg catccactac
      241 ttttttattt acttgttttt taaagtttgt ttgatactcg ttatattttt ttacagtttc
      301 agcgttttta gtttgtgcat gagattcacc ggcaaacgtt gataccgtta aactaatagc
      361 agctatgctt aaaattgatt tcccaataaa attctttttc ataattaata acacctatcc
      421 tcaaatattt attttgtata tatttataat aatagacaga tacaaaaaac tacattaact
      481 atttattaac taaacattaa tatttgtgtc actacatttc tgaatttcaa gaattttgct
      541 actccatatt ttaaccatca aattattgat ataatttatg actttagaaa tacttttaaa
      601 tatgtatacg attcatgatt ttaactaaaa tttcaaattt cctgacttct tggaatctta
      661 attttatact ttttaataat acacttttaa ccaaataatc aacatgattc aaccttatag
      721 ggtcatcaac aatcacattc tccaaaatta caattctttt cataacataa atgtgttatt
      781 cattccaatt atcttataaa attgtcagaa atacttttaa acaaacattc atgacacgac
      841 ctcctagtta aatcagtcta taaactaaaa aaagccatag ctacttctat ttaaaaagta
      901 gtatgactga ttgtttcgtg atgtcgattt ttgaaattga tggtgccatg taaagtttga
      961 tcatcaataa taacttcagc atatgtttct tccattgaac ttctagattg agatatactt
     1021 ccaggattaa taacatgaac accattaata tactcatatt ttgccacatg tgtatgtcca
     1081 taaaatgcaa acaaacaacc taattcaagt cctttttcag ctaataaatc tcttgttcga
     1141 ttgacttgat ataaatgtcc atgagtataa aaggctttca cgtcattata ttttgcgacc
     1201 gcttcatttt caaattctgg gtaaaaatca caattgcctt ttactctatt aaataagcta
     1261 agttccgtat catcatacgc gaattctgaa tctcctaaat gtaaatatac atctgcatct
     1321 gggtgcattt cataaatttg atataaaacg cctgattcag tatggttatc actcacaata
     1381 atccatttag acattttttt caccttcaag aaaagcttga agtaaattaa tcgcatttcg
     1441 tctatgacta atttgccctt tttgttcttt tgaaagttga gccatggttt tatctaattt
     1501 cggtacataa aatatcggat catatccgaa accattttcg ccatattttc catctgcaat
     1561 ttcacctgaa acagtacctt taaatacttt tgtttccata tcagggccac tcatacttat
     1621 gacacaaaca aattgcgcac gacgatcagt tgtattacca agcttattta ataatttttc
     1681 aatatttgct tcatcacttt tattttcacc agcataacgt gcagagtata tacctggctc
     1741 accatttaat gcaaaaactt ctagtccact gtcatcagct atgaccgttt tattcaatgc
     1801 ttttgcagca gcttctgatt ttaatatagc attttcttca aatgttgatc ctgtttcttc
     1861 cacatcaaaa tctggtatta gttctgaaat acctattacg tggtaatctg gaaatattac
     1921 tttaaagtca tttattttcc cttgattatt cgatgcaata acaatctctt tcattaaaaa
     1981 tcctcctagt cattcactga tatacgttcc acattgacag ataaatttaa ccactctttg
     2041 ataatgttag taatgtgagt aggatcacct gttgcaaaaa atcgatgatc tggatgttca
     2101 gtataacttg catgttcatt actaaatgtt agcaatgcac taacttcacg agccgtttct
     2161 aatccagacg aaatcactgt ctttttacca ccaaaataat catagatagg tttatagagc
     2221 aatggatagt gggtacatcc taaaatgaca gtatcagact cactattacg ccaacgtttc
     2281 agtgtttgat gaatgacaat gcttgtaatt gttggatcac tatatctcat ttgttctaca
     2341 agtggcacaa aacctggaca ggcaacgcca tgtacctcta catgtggatt gatacgttta
     2401 atatgcgtac gatatgcttc agatttaatt gtgccttccg ttcctagtac taatacattt
     2461 tgatttctag tagtcattat tgctgttcta gcacctggtt caattacgcc aatcactggg
     2521 attgataagg tcttttgtaa atattctaaa gctacagcag ttgctgtatt acaagcaatc
     2581 acgagcattt ttatatcaaa ttccattaat ttacgagcga tttcaactgt atattgtttt
     2641 acttgttctc ctggtcttgg cccatatgga catcgcccaa tatcacctaa gtaataaatc
     2701 gtctcatttg gcaactgacg cataatttct ttagctactg tcaaacctcc gacaccagag
     2761 tctattacac ctattggttt attcatattc ggtcatcctt acttttaatt taatttttag
     2821 tttatcataa ctaagcattg gattttagta ttatgcactg tgtttaccat ttttgtcatt
     2881 ataatattta ttttaaatca gcccactatc atattgtcat gtaatctgct tattaaaaaa
     2941 atcccttcca agttattgtg tatctccatt caatttaatt ttgaaaggaa cataacattt
     3001 taactcaaaa gggattaatt tttattctac ttcatggtct gaaccaaaga atgatttaaa
     3061 catgtggaat gttgtttctc tgttcattgc tgcaatggat gttgttaatg gaataccttt
     3121 agggcaagca ttaacacagt tttgtgaatt accacactgc tgtaagccac cagtacccat
     3181 taatgcattt aaacgttcat ctttagtcat agatcctgtt gggtgcaaat taaacaaacg
     3241 aacttgcgag attgcttgcg caccaacgaa tttattattt tcagtaacat taggacaaac
     3301 ctctaaacat acaccacatg tcatacattt agataattca taagctgttt gacgtttttt
     3361 ctctggcata cgtggtcccg gacctaaatc atacgttcca tcaattggga tccatgcttt
     3421 catacgtttt aagttatcga acattctaga acgatcaact tgtaagtcac ggataactgg
     3481 gaaagtattc attggctcta aacgaatagg ttgttctaat tgatcaacaa tcgcagaaca
     3541 agattgtctt gcacgaccat tgataaccat agaacatgct ccacatactt cttctaagca
     3601 gttcatatcc cagacaacag gcgttgtttt ttcaccttta atattaactg ggttacgtct
     3661 aatttccatt aaacaagcaa tgacgtttaa attttcacga tatggaattt caaatgtttc
     3721 ttcataaggc ttagaatcac ttgtatcttg tcgtttaata attaatttta ctgttttttg
     3781 tttcggttta gattgtgttt catgttgtgg agtgtttttc actgattgtt cagtcattat
     3841 tttttacccc ctttagactt acttgtgtaa tcacgtttac gaggtggtat taaactcaca
     3901 tcgacgtcat cataagtaaa ctgtggtttt tcaaatgcgc cttggaatga ggccattgtc
     3961 gtttttaacc actcttcatc attacgctct gggaattctg gtttataatg ggcaccgcgt
     4021 gattcgttac ggttatatgc accaatcgta ataacacgtg caagtactaa catgttccat
     4081 agttgacggg taaagaatac cgcttggtta ctccaagttt gagtatcttc catatcaata
     4141 tcttcataac gtttcatcaa ttcaacaatc tttttatctg tttctaacag tttttcattt
     4201 tcacgaacaa cagttacatt tgctgtcata atttcaccaa gttcacggtg taatttatat
     4261 gcattttctg taccgcgcat agctaataat ttatcaaaac gttcttgctc ttcagcttta
     4321 cgtttttcaa aaatactttc gtccatatca gtatatgatc gatcaatatt tgaaatataa
     4381 tcaatcgcgt ttggacctgc tactgtacca ccataaatcg ctgataacaa tgaattggca
     4441 cctaagcggt taccaccatg ttgagagaag tcacattctc cagctgcaaa taacccttta
     4501 atatttgtca tttgatcata atctacatat agaccaccca ttgaatagtg gacagctggg
     4561 aaaatcttca ttggtacttt gcgtgggtca tcaccagtga atttttcata aatttcaatg
     4621 ataccaccta gttttacatc taactcatgt ggatctttat gtgacaaatc aagatatacc
     4681 atgttttcgc catttatacc taatttttgg ttaatacata catcgaaaat ttcacgcgtt
     4741 gcgatatcac gaggtactaa gttaccataa tcaggatatt tctcttctaa gaagtaccaa
     4801 ggcttaccat ctttatatgt ccaaattcgt ccaccttcac cacgtgctga ttcactcatt
     4861 agtcgcagtt tatcatcacc agggattgca gtaggatgaa tttgaatgaa ctcaccatta
     4921 gcataaatag cgccttgttg gtaaacaatg gaagccgctg atcctgtatt aatcattgag
     4981 tttgttgttt taccgaaaat aataccaggg ccacccgttg ccataataac tgcatctgaa
     5041 ccaaatgttt caatctcagc agttgtcata ttttgtgcaa cgatacctct tgcactatca
     5101 tcatcacctt taactatgcc aaggaattcc catccttcat acttcgtaac taatccatct
     5161 acttcatatg cacgaacttg ttcatccaat gcatataata attgttgtcc agttgttgcc
     5221 cctgcatatg ctgttctgtg gtgtaatgta ccaccgaaac gtctaaaatc taatagacct
     5281 tcatttgttc tattgaacat tacgcccata cggtctaata aatgaataat tttaggtgct
     5341 gcctctgtca tcgctttaac aggtggttgg tttgcaagga agtcgccacc atacactgta
     5401 tcatcaaagt gaatccaagg agaatcgcct tcccctttag tattgaccgc accattaatg
     5461 ccaccttggg cacaaacaga gtgcgaacgc tttactggta caactgagaa caaatctaca
     5521 tgtgcacctt tttctgccgc tttaattgtt gacattaagc ccgctaggcc acctccgaca
     5581 acaataagat gtttctctgc cataaaaatt tcactcccct aaattttcaa tctatatttg
     5641 ttaaatgcga tgtattacat aaaggcaata attgcagtaa caccaatata cgaaataact
     5701 aagaatacga ttaatgaaac ccatgtaaat actcgttgtg attttggaga ttgaagtcca
     5761 ccccaagtaa ctaaaaatga ccataagcca tttgcaaagt ggaacacaac agcaataata
     5821 caaataatat aaaatattgc ccatccagga tgttgcaatg tttcgtgcat taaatcgtaa
     5881 ttcacttctt tgccgtaaaa tgctttttgt aaacgtgttt gccataaatg gataccaata
     5941 aagataaatg ttaagatacc actcactctt tggaagaaga acatccagtt tctaaaaatc
     6001 gagtaatgtc caacattttc ttttgctgta aatgcaatgt gtataccaaa caaaccgtga
     6061 tataacaacg gaatgtatat aaataaaaat tctacaataa ttagaaatgg taatgattcc
     6121 ataaagttag atgccttatt aaacgcttca gcaccttgtg ttgcttggtg attcactaat
     6181 aaatgaacga ccaaaaatgc acctattggg ataataccta ataacgagtg aatacgtctt
     6241 agataaaatt cattttttga ttgagccaaa aggagtcccc cctgtgaacg aatatttaat
     6301 ttattgagct atttatatta aacgtacgct taacccccta aagtgatatc gtttctagcg
     6361 ttgtcattat cattaagcga aacattttaa agacaaatac acactgcacg atcaccaaac
     6421 tgcatgtcga acaatgtaac atttggattc gatattttag attgcttgtg atgataaact
     6481 ttctcattta gaaaacgctt ccacgtacat tcaaaaaaat aactttgtta accatattgt
     6541 aacattattt catatatttt ggggcatgag aatgattctc acgccctgta atttatttat
     6601 gcaattgttc atgtaggttc tttgcaacgt tttcaggaat acctatattt ttaaaatctt
     6661 caagtgtagc ttccttcatt ttcttgattg aaccgaatga acgcaataat aatgttttac
     6721 gtttgttacc gataccatct atatcatcaa gtattgattt caagcctgtc ttttgacgtg
     6781 tttgtctatg aaatgtgatt gcgaatctgt gaacctcatc ttggatacgg tgcaacaaat
     6841 aaaatgcctg gctatttttc ttcagtggta caatttctgc actagcgcca tataataatt
     6901 cagatgtttg gtgtttatca tttttctgca aacctgcaac aggaatatca agacctaatt
     6961 cgttttgtag cacatcaata accccgttca tatgtccttt accaccatct actattatta
     7021 aatcaggtaa tggtaatcct tcgtttaaaa cgcgagaata tcgtcgtctt actacttctc
     7081 tcattgattt gtaatcatct ggacctttaa ccgttttgat tttatacttt ctataatttt
     7141 tcttatctgg tttaccgtcg acaaatgtaa ccattgctga cactggatcc acaccttgaa
     7201 tattagaatt atcgaatgct tcaattctaa ttggtgtttg aattcccatt tgtgttccaa
     7261 gttcttcaat agctttaatc gttcttgact catcacgtga tattaattca aatttattat
     7321 ttaaggatac tttagcgtta tgtgcagcta ggtcaaccat atcttttttg ggacctcgcg
     7381 cgggttgaac gattttagtg tccacaacag attgaatcat ttctttatcc aaattacgtg
     7441 gtacatgaac ttccttaggt aaaatatgtt ggtttaagct ataaaattgt ccaataaatg
     7501 tataaaattc ttcttcttct gtttgctgta atggaatcat cgttgtatct cgctttatca
     7561 tattaccttg tcgtataaag aaaacttgga tacacatcca tcctttatca acactataac
     7621 caaagacatc acgaatcgtt ttatctgatg acataatttt ttgtttgttt gtcagatttt
     7681 gaatatgttg aattaaatct ctatattctt tagcccgttc aaaatcaagt gattcacttg
     7741 cagttaacat tcgctcttct aaacttttta aaattgtttt gtcttcccca ttcagaaaat
     7801 cagtaatttc cttcgtcatt tgtgcgtatt tactcaaatc aacgtcatat acacatggtc
     7861 ctaaacattg tccaatatgg taataaagac ataatttatc tggcatctta tcacatttgc
     7921 gatatggata tattctgtct aataactttt tagtttcttg agcagaatat gcattcggat
     7981 acggtccgaa atatttgcca gtaccttgtt ttacagttct cgtcacttgt agtctaggat
     8041 atttttcctt cgtaatttta ataaatggat aacttttatc atcctttaat aatatattat
     8101 atcttggttg atattgtttt atcagattca attccagtaa aagtgactct gtttcacttg
     8161 acgtgacaat aaactcaaaa cgacgtattt caccaaccag tctcgttgtt ttagcatcat
     8221 gagcacccgt aaaatatgat ctcaatcgat ttcttagctt tttagctttg ccaacatata
     8281 tcacttgatc attacgatct ttcattaaat agcagcctgg ttccataggt acgacattta
     8341 atttattttt aattcgttgc ttatagtctt ccaataatat ccccccttct aattttaaaa
     8401 tgaatgatgc tacaaaagtg cttcaaattt atcaccacta attctagtta ataatcaaca
     8461 tcttcgagta tgactcttca tgcacatcat gtaatattga acattgtttt atcaaaccaa
     8521 ctgctacaaa tctatcagca tgctagatta tcatttaacg ttcatagatt tattaatccc
     8581 accattctca agtcgtaaac aatatgatga cttatcattc aactttttat attaaaaaca
     8641 caaacaaaaa ctggagcaat tcattaaaga aatgccccag tcgtcattgg ttgtaactta
     8701 taaatgttta tctaaaactt cagctaagtt ttcttttggt tggaaaccaa caactttatc
     8761 aactggttga ccgtctttaa agacgattaa tgttggaata ctcatcactt catatttagc
     8821 tgctgttgat ggattttcat caacatctaa ttttaaaatg tcagctttac cttcatagtc
     8881 agctgctaat tcttctaata ccggagcgat cattttacat ggaccacacc atgttgccca
     8941 aaaatctatt aattgtacac cagattctac ttttgaatca aaatctgcat ctgttacttt
     9001 tacgattgcc ataaatgcca atcctcctta attattatta tgatgttcaa attatatcag
     9061 ataacatgct tcatgtattt ctatttgctc aatttttatg cttatttcaa ataacatgtt
     9121 tgaaatgtat tcttgttgct ttacattatt ttaactgcag ctatttaatt tatcaaatta
     9181 taatttattt tagtgttgca acggtaacgc caaatccacc ttcgcttggc ataccacctc
     9241 taaagtcact aacactttta tgcttcttca aatgttgttg tacacctttt tgaagtgcac
     9301 ctgtaccttt accatgaatg atataaactt gttcgtaatt acttaaaacg gcttgatcta
     9361 aatattgatc tagttcaatt aaagcatcct cataacgata gcctcgtaag tcaagttcag
     9421 ttttaattgt ttgacgattt tgacgtgtaa ccattttcgt tggcttaact ttttcttttt
     9481 gttttttctc taaatcttca ataggtaact tcattttaat aattcccatt tgaacaattg
     9541 cttcttcatc attgacaatt tctaaaactt cacccttttg accgtaagat aatactttta
     9601 cttcatcacc agcaacaatt ttatcgtatt tttgcttttg tacattttgc tttatagatt
     9661 tcgcttcata atgatcatct aatcgtttct tcttatcaat caattcatgt tctttaacat
     9721 ctgcaccttt ttgttcacgc aattgtctta agtctttaat aatatcgtca gcttcttttg
     9781 ttgcagcttt aatcttctga tttgcttttt ctttcgcttc ctctattaga gacttttcat
     9841 aattttggaa ttgttgatac tgcttagata aatcatcatg cacttgctcc gcttctttta
     9901 caagacggtc cagttctaac ctctgtgtct ctacacgttt ataattacgc tctaatgatt
     9961 caatcatttc atttatttct ttttcatcag taccaatcat cgtcttagcc ttattaataa
    10021 tattcaaact aagacctaac tttttagaaa tgtcaaaagc atttgaacga cccggcacac
    10081 ccattaataa cttatacgtt ggactcaaag tatctacatc aaattctaca ctcgcattca
    10141 taacgccttc tcgattataa ctatatgctt taagttcagg atagtgcgtc gttgccatta
    10201 ctagagaacc aatttttcta acatgatcta aaatgctcat tgctaatgca gcaccttcac
    10261 ttggatctgt acctgcacct aattcatcaa ataaaactaa actatgtttg tctgcatgct
    10321 ttaaaatttc aactatattc gtcatatgag atgaaaaagt tgataatgat tgttctattg
    10381 attgttcatc tccgatatcg caatatacat ttttaaatac acttaactga ctaccatcaa
    10441 gtgtgggaat caacaatcct gattgagcca taacaataat taaacctaat gtttttaatg
    10501 ttacagtttt accacctgta ttcggtcctg taataattac cgtttcaata tcttccataa
    10561 attcgatggt attagctaca acagtctcac gatttaataa tggatggtat gctttaggta
    10621 aatatacagt acgttcctct ttaaatatcg gctttgttcc tttaatactt ctactatatc
    10681 tcgcttttgc gattaaaaaa tctaactgcc ccatgacttg ttctgccaca agtagtgcat
    10741 ctttgtccgc agccacataa ccagttagtt gcgttaaaat gcgttctttt tcaattgctt
    10801 cgtcatgacg taatcgacta atttgattat tcatttcaac aactgatgat ggctcaatat
    10861 acaatgtttg tcctgaagca gattgatcat gtacaatccc attaaaatct tgtcgatatt
    10921 cagctttgac aggtataacg tttctttcat tcctaactgt tacaatagca tctgataatt
    10981 ttttctgatt tgcttggctt ttaacaatac ggtccaaatt ttgtctaata cgttgattcg
    11041 tgctagaaat tttacttcta atcccttgca attcataact cgcattatca tataaatcat
    11101 acgtatcgca tgtttcattt atttgatgaa aaagatcagt taacacaggt aattgattca
    11161 tcttgtcatc taatattggg tatttaacac cttcatcttc ttcaaccaat tgattataaa
    11221 atgtcttaaa ttgattttgt acttgaatta atctttttat caagttaagc tctgatacat
    11281 ttaaaacgcc gccaatatca gcgcgatgaa tgaatgctga tactttagat aagccactca
    11341 agcttggtaa acgatgctta ttatagattt gagcaatctc atccgtttct tccatttgaa
    11401 aaacaaccgt ttcaaaatta gtagctggca tcatttgatt gaccttttcc aagcctaagt
    11461 cactaatagt ttcattggca acgagtgatt ttattttttc aaattctaag acgtctaatg
    11521 ttttttgtct cataaaatcc ctctatttct taagttttat attattttca ataaagtctt
    11581 taaaagcttc acgcgataat gcgttaatca ctctatcttt tgttacaaat cctttttgcg
    11641 cagttgctac gccataattc ataaaatcta aatgatttgt atgatgcgca tcagtgttaa
    11701 tagttaattt cacatttgga tatttacgaa cgatatcagc gttcagatcc agtcgatgtg
    11761 gattggcatt aatttctaat actgtattcg tttcttcagc taatgccatt aattgttcaa
    11821 tattcggttt ataaccatct cttctaccta taatacgccc tgttggatgc gctaaatgtc
    11881 gcacgtatgg attgcgacat gcattagcta atcgttccat aatttgttct tctgattggt
    11941 taaagctttg atgaatagct ccaattacat aatcaagttg tgctaaaatt tcatcatcat
    12001 aatccagcga gccatcaggt aatatatcca tttctgtacc tgaataaata tcaatttcac
    12061 tatattcttt atctaaagcc ttaatttctt cgttttgtct taaaagtctt tctacttgta
    12121 agccattagc aacacgtaaa ctttgtgaat gatcagtaat taccatgaat ttataacctt
    12181 ttgcgatatt tgcttctacc atgtctcgaa tagaaaacgc accatcacta tacgttgtat
    12241 gcatatgaat atcaccatta atatcatcta ttgtaatgat attacttaga tctttatcaa
    12301 attcgctacc atcttctcgc atagcaggtg gtataaaatt cacattaaaa tgttcatata
    12361 tcttggcttc actatcatat tgaattaatg taccatcagc ttgttcaatt ccatattcac
    12421 ttactttttc atcacgtgct ttagcaagtt gtcgaattct tatattatgt tcttttgacc
    12481 cagtaaaatg ctgcaatgta tgataaaaag cacttggttc aattaatcga aaatcgacac
    12541 caatcgtttc atcatcatac gctaattcta atgaaacttt tgtgttcccc actgcaactt
    12601 cttttacttt attgggaata tttaataatt gctgctgcac tgcttttggg ttatcggtac
    12661 ttattatgaa atctaaatct ttgctcattt ctttaaaacg acggaagctt cctgcagatg
    12721 aatattgatc gatataattt aatgtatcta tataatcaat gatttcttga ttaagtcttc
    12781 tcatttgatc aattggatat ctatctttct tagcaccaag ttgtttcaca gcttctaata
    12841 tgttttgttc cgttttctta gcaaatccgc ttaattcact aacttttcca ttttcacaag
    12901 caacttgaag tgacgcttta tcaacaatat tcaactcttt atatagctta gcaattttct
    12961 tgcttccaag tccttgaatt ttcaaaagtg gaataagacc ttccggaact tcttcctgta
    13021 attgctgtaa atactgagat tcaccggtct cacggtaatc attgattact tctgcaacac
    13081 ctttaccaat gccttttaac tccgttacat cagatatttc atctaatggt cgttcatcta
    13141 attcaagact ttgagctgct tttcgatacg ctgatatttt aaaagtattt tcccctttta
    13201 attccatata agtagcaatt tgttctaata gtttgataac atcttttttt gtcataataa
    13261 cactccataa aaagaagacc aggacgtatc attaatatat acctttgtcc tgacctctta
    13321 tgataattta taaattaatg gtgaaagccg ataaatatgg gatgtgaaaa atcaatgaat
    13381 gactcacaat agatatttta agctgatgtt gtaatgcttc gttaggatat aatgccaata
    13441 gacataacgt aaagtgtaag acaattatcg tcataaacac accaactatc attcccattg
    13501 cacgactgaa aatatgaatg ttttgatacg ctattatttt atcaaaagtt acgataatta
    13561 gatataaaat gaacttacaa aacaatgtaa tcattaaaaa agctacaatc gcttcaaatc
    13621 gattttgtag atgattaaaa tgaaacgcaa aagttgtatt aaatgctgtt gttttaggat
    13681 atggaataaa tacaattaat ctttctacaa tagctttgta aaattgactg gcaatccaca
    13741 atgatacaat cgttgcactc aaatgtatca tagataacca aaaacctcgt ctgaatccaa
    13801 ccatgacaaa atacacaaag aaaatgatta tgataaaatc aatgaccatt tattgctcac
    13861 gctgctgcaa tttgtgaatt tgttgtttca aacgtcgatt ttcttcttct agtagtactt
    13921 tttcatgcat aatattcaca gcagttagta ttgcttttct tgaagtatct aaacctgctg
    13981 ctttataccc taattctttt attttatcat caactaaatg tgctacatat cgtatgtgct
    14041 ctgggttatc ttccccaaca attgtaaaaa gctgatcatt aattgataca tttaccttgt
    14101 ttttaaactg tgccatttat aatttctcct gatccttttt ttaaaatcta aattcacgtt
    14161 ataaaatatg actggatagt
//
LOCUS       DAHHYJ010000068        14122 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000068 DAHHYJ010000000
VERSION     DAHHYJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14122
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.068"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(171..1064)
                     /locus_tag="NBK22_001508"
     CDS             complement(171..1064)
                     /locus_tag="NBK22_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD2414697.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNTPNNNHQNQSRTNDNHQHANQRDLNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYNYIH"
     gene            1312..1605
                     /gene="esxA"
                     /locus_tag="NBK22_001509"
     CDS             1312..1605
                     /gene="esxA"
                     /locus_tag="NBK22_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCD2414698.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEIAANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            1688..4717
                     /gene="esaA"
                     /locus_tag="NBK22_001510"
     CDS             1688..4717
                     /gene="esaA"
                     /locus_tag="NBK22_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCD2414699.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDKD
                     TTFIYNMSQQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQEEPNDKKATSVVDVT
                     LNHNFDDCLSKDALSQQLSALSRFDAHYQMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKTIADSVSGQLNQL
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKDNELFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLVEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFIKHFKKERLI"
     gene            4717..5175
                     /gene="essA"
                     /locus_tag="NBK22_001511"
     CDS             4717..5175
                     /gene="essA"
                     /locus_tag="NBK22_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCD2414700.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKEVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            5147..5389
                     /gene="esaB"
                     /locus_tag="NBK22_001512"
     CDS             5147..5389
                     /gene="esaB"
                     /locus_tag="NBK22_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCD2414701.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLISLVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILELL"
     gene            5402..6736
                     /gene="essB"
                     /locus_tag="NBK22_001513"
     CDS             5402..6736
                     /gene="essB"
                     /locus_tag="NBK22_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCD2414702.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLVPDELFFTRDGLPIAKTRGLQNVVDPLPVSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTAFLDEQYQKQEQDYSQNYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKEDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAINIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            6758..11203
                     /gene="essC"
                     /locus_tag="NBK22_001514"
     CDS             6758..11203
                     /gene="essC"
                     /locus_tag="NBK22_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCD2414703.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERPDITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLHYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WAHWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYDIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRKIHDIISERK
                     RLLSQERVVNIEQYNKETGNSIPNIFLIIDNYDTVKESPFMEEYEEMMSKVTREGLAL
                     GVYIILSGSRSSAIKSAIFTNIKTRVALYLFENNELTNIIGSYKKGVKDVKGRAAIND
                     DNFTQFQIAQPFELAEGQTYNERIKNEVAQMKEFYVGDYPKHIPMMPDKVLMEDIREA
                     YDLEKIIHEEHKLPLGLDFEDVELVSLDLTSPSILTARTPNDLEIVNNRVLDGMSKLK
                     ESVTTILVDADENMSDKSSIVNSYYASSEDLQLIKQGFIVEIKKRLNSERSKESVKIV
                     FINNLKAFISITGLNEKDIRYILSEGPKVNVYTIISSMYNDILGTFDRESKLARQIIN
                     QAVIVTRLYDQEFLQAKITNREPLLKPYEMYYFNKNEHIKIKLIQ"
     gene            11215..11511
                     /locus_tag="NBK22_001515"
     CDS             11215..11511
                     /locus_tag="NBK22_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCD2414704.1"
                     /translation="MGEIKVETSSTIGKIESISNAGSNVSFDNVSDSLEYTNISPFTG
                     FAAASSTLTTAISNYHSIITQDTEAMKTAVNDFRDKDADIAGQIDGNNNLGAGL"
     gene            11529..11924
                     /locus_tag="NBK22_001516"
     CDS             11529..11924
                     /locus_tag="NBK22_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HCD2414705.1"
                     /translation="MANKEELKKRKARCIEQKHSKESELKSLQGDKKRLQDAVNSAQT
                     IIEDFNSEKKTYDGIEIEKNQWSGQTRNKSDDNRTNLNTSIDKYKEKFDKALNQMKKD
                     LENLESKIEGVETDISNIAAEIQNIDSQL"
     gene            11950..13503
                     /locus_tag="NBK22_001517"
     CDS             11950..13503
                     /locus_tag="NBK22_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2414706.1"
                     /translation="MGNKIKMSEVNNFNASLQKSLSSLSTNTKTLKNNINKLKDDSEF
                     KGKTASNINSYNQSFHIETISRIEKIKEEFEKKLKDAIEDFQNSVDNDHNAILDEDAI
                     EKYKGEVKKSIEQIESIKTRMNSTIGGVHDLTTAKTISGKDVKEKGETFNKQITDTLD
                     NFNNFQSLHFLDDSELLSMITPVSTMASKVKNMPSNRSTISGNAGYIKSEYKLHSENN
                     QFRELKKELEKYESAIYGGNKYRKTVKSMIEIKNALIVAYIAGDGNIFKGNELLAKNQ
                     LGKLGNKKLKLINATMQTNVENIEGKRLVKAAKFILDKNNKMKFSEKLKAGFKMTRNY
                     SDTEFKQLEKVAGKYNGKSAMKRGAKAAWESMSQKQLREFLKNPKNFKNICLDEVKDF
                     LGKNKLGKFLKSMRYAGKLLGPLGAVAAVANNVVNEKSGQKKIVGSIVDLGAIGGSAA
                     TGTAIGAGIGGPFGAAIGAAIGTMVGISTDIKIVGGKSLTDLAKDKLNNTVSSIRSNG
                     FGKTLSNVF"
     gene            13517..>14122
                     /locus_tag="NBK22_001518"
     CDS             13517..>14122
                     /locus_tag="NBK22_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCD2414707.1"
                     /translation="MNNTKENIDTLRKPGAQALSLASLFMILFSCLTFFFGLDYERFP
                     NYLKITTIIELIIIIISLLQWIRFIDFEKESAQKYKKIYARFLVVINVLTTITAVFAT
                     CNLYYFVAVQNHYDLFNYWLMGTISIIISYLLLVIGGMFTLLKLPKVTKRWGGKTKTH
                     FGLLLTALSAFIYIERIIEYILVPNVVESKFVIMVSIIIIAC"
BASE COUNT         5502 a         1900 c         2554 g         4166 t
ORIGIN      
        1 taatgattat ttaaaaatgt cggttaactt taaggtgaca agttattgca attttgaagg
       61 tagacatcat attttataat cgaataacat acaaaataaa gcgaccaatt ttgcagtgtt
      121 acgaattgta aactgcgaac tggtcgcttt ttattgatgt gatgattaat ttaatggatg
      181 taattatatg atgaaacttc tgaagcagag atggttcttg atgaaacgat atattcacca
      241 atccaattca tttctgaaat tagaatactt ccatcaatat taactttttc aacgtaggct
      301 acatgaccaa atggaccatt tactgtttgt aaaattgatc ctcgtgttgg gtgtctatct
      361 actttgaagc cattgcttga agcttggcct gcccagtttt tagcatctcc ccaaaatgta
      421 ctaatcgtgt gtccatcttt ggcacgttta tcaaagacat accatgtaca ttgtccagca
      481 gtatataagt tgttcttact tgtgataaga ggctgatcaa tgattttacc gttacctaat
      541 gctaaaggtt taccgtcagc agtctttgct ttgtcattaa attcggcgat ttgtaattcg
      601 tcatacaatt cgtctaaatc gatgcctgta ataagccctt tgttatcttt tgaaaaagcg
      661 ttatttaatt catcgtcatt gtcttcgaca ttcggtattg ctggtgtcaa aggattgctt
      721 ggtgacgttt gaggcggtgt gtgtgaatca attgcgtcat taatgtgcgt atactgacca
      781 cttaatgaag aatggtactg attgttgtta agatcacgtt gatttgcgtg ttgatgattg
      841 tcgtttgtac gtgactggtt ttgatgattg ttgtttggcg tgttgttttt gtcatatgta
      901 taagtatacg cgccggtgtc tttattcact ttgaactgtg cgtttgggtg tgctttcttt
      961 gcttcttcta atgttttgct atcattcgta tatgcttgag ccgagttagg cgacatacta
     1021 aataaagtaa gagttgtcat cgtcagtaaa attgttttct tcataataac catttaatcc
     1081 tttatgtatt taatttaatt ttagtataca catttatatt acaaaaatga atggttaatt
     1141 aaaaatatat gggtatattc aatatattta tttaaaaaaa gctaaaaata cttaaaaaac
     1201 tatatacata tactaataat ttatatatta tttgagtaag gagcactttt tcaaaaaata
     1261 gtgtccctaa aaagttttga taaacttaaa atattcagga ggtttctagt tatggcaatg
     1321 attaagatga gtccagagga aatcagagca aaatcgcaat cttacgggca aggttcagac
     1381 caaatccgtc aaattttatc tgatttaaca cgtgcacaag gtgaaattgc agcgaactgg
     1441 gaaggtcaag ctttcagccg tttcgaagag caattccaac aacttagtcc taaagtagaa
     1501 aaatttgcac aattattaga agaaattaaa caacaattga atagcactgc tgatgccgtt
     1561 caagaacaag accaacaact ttctaataat ttcggtttgc aataagcatt ctgaaattgg
     1621 caaagtcaca ttttctaatg tggctttgct tatcattttt taagaaaaca actgaaagga
     1681 aataagcatg aaaaagaaaa attggattta tgcattaatt gtcactttaa ttattataat
     1741 tgccatagtt agtatgatat tttttgttca aacaaaatat ggagatcaat cagaaaaagg
     1801 atcccaaagt gtaagtaata aaaataataa aatacatatc gcaattgtta acgaggatca
     1861 accaacgaca tataacggta aaaaagttga gctgggtcaa gcatttatta aaaggttagc
     1921 aaatgagaaa aactataaat ttgaaacagt aacaagaaac gttgctgagt ctggtttgaa
     1981 aaatggtgga taccaagtca tgattgttat tccagaaaac ttttcaaaat tggcaatgca
     2041 attagacgct aaaacaccat cgaaaatatc gctacagtat aaaacagctg taggacaaaa
     2101 agaagaagta gctaaaaaca cagaaaaagt tgtaagtaat gtacttaacg actttaacaa
     2161 aaacttagtc gaaatttatt taacaagcat cattgataat ttacataatg cacaaaaaaa
     2221 tgttggcgct attatgacgc gtgaacatgg tgtgaatagt aaattctcga attacttatt
     2281 aaatccaatt aacgacttcc cggaattatt tacagatacg cttgtaaatt caatttctgc
     2341 aaacaaagac attacaaaat ggttccaaac atacaataaa tcattattga gtgcgaattc
     2401 agatacgttc agggtgaaca cagattataa tgtttcgact ttaattgaaa aacaaaattc
     2461 attatttgac gagcacaata cagcgatgga taaaatgtta caagattata aatcgcaaaa
     2521 agatagtgtg gaacttgata actatatcaa tgcattaaaa cagatggaca gccaaattga
     2581 tcaacaatca agtatgcaag atacaggtaa agaagaatat aaacaaactg ttaaagaaaa
     2641 cttagataaa ttaagagaaa tcattcaatc acaagagtca ccattttcaa aaggtatgat
     2701 tgaagactat cgtaagcaat taacagaatc actgcaagat gagcttgcaa ataacaaaga
     2761 cttacaagat gcgctaaaca gtattaaaat gaacaatgct caatttgctg aaaatctaga
     2821 aaaacagcta catgatgata tcgttaaaga accagataaa gatacaacat ttatctataa
     2881 catgtctcaa caagacttta tagctgcagg tttaaatgag gatgaagcta ataaatacga
     2941 agcaattgtc aaagaagcaa aacgttataa aaacgaatat aatttgaaaa aaccgttagc
     3001 agaacacatt aatttaacag attacgataa ccaagttgcg caagacacaa gtagtttgat
     3061 taatgatggt gtcaaagtgc aacgtactga aacgattaaa agtaatgata ttaatcaatt
     3121 aactgttgca acagatcctc attttaattt tgaaggcgac attaaaatta atggtaaaaa
     3181 atatgacatt aaggatcaaa gtgttcaact cgatacatct aacaaggaat ataaagttga
     3241 agtcaatggc gttgctaaat tgaaaaagga tgctgagaaa gatttcttaa aagataaaac
     3301 aatgcattta caattgttat ttggacaagc aaatcgtcaa gaagaaccaa atgataagaa
     3361 agcaacgagt gttgtggacg taacattgaa tcataacttt gatgattgct tatcgaaaga
     3421 tgcattaagc cagcaattga gtgcgttatc taggtttgat gcacattatc aaatgtacac
     3481 agatacaaaa ggcagagagg ataaaccatt cgataacaaa cgtttaattg atatgatggt
     3541 tgaccaagtt atcaatgaca tggaaagttt caaagacgat aaagtagctg tgttacatca
     3601 aattgattca atggaagaaa actcagacaa actgattgat gacattttaa ataacaaaaa
     3661 gaatacaaca aaaaataaag aagatatttc taagctgatt gatcagttag aaaacgttaa
     3721 aaagacattt gctgaagagc cacaagaacc aaaaattgat aaaggcaaaa atgatgaatt
     3781 taatacgatg tcttcaaatt tagataaaga aattagtaga atttctgaga aaagtacgca
     3841 attgctatca gatacacaag aatcaaaaac aattgcagat tcagttagtg gacaattaaa
     3901 tcaattagat aataatgtga ataaactaca tgcgacaggt cgagcattag gcgtaagagc
     3961 gaatgatttg aaccgtcaaa tggctaaaaa cgataaagat aatgagttgt tcgctaaaga
     4021 gtttaaaaaa gtattacaaa attctaaaga tggcgacaga caaaaccaag cattaaaagc
     4081 atttatgagt aatccggttc aaaagaaaaa cttagaaaat gttttagcta ataatggtaa
     4141 tacagacgtg atttcaccga cattattcgt attattgatg tatttactat caatgattac
     4201 agcatatatt ttctatagtt atgaacgtgc caaaggacaa atgaatttca ttaaagatga
     4261 ttatagtagt aaaaaccatc tttggaataa tgtcattacg tcaggtgtta ttggtacaac
     4321 tggtttggta gaaggattaa ttgtcggttt aattgcaatg aataagttcc atgtattagc
     4381 tggctataga gcgaaattca tcttaatggt gattttaact atgatggtct tcgtacttat
     4441 taatacgtat ttactaagac aggtaaaatc tatcggtatg ttcttaatga ttgctgcatt
     4501 gggtctatac tttgtagcta tgaataattt gaaagcggct ggacaaggtg tgactaataa
     4561 aatttcacca ttgtcttata tcgataacat gttcttcaat tatttaaatg cagagcatcc
     4621 tataggcttg gcgctagtaa tattaacagt acttgtgatt attggttttg tactgaacat
     4681 gtttataaaa cactttaaga aagagagatt aatctaatgt tgatgaatag cgtgattgct
     4741 ttaacttttt taacagcatc tagcaataat ggcggactta atattgacgt gcaacaagaa
     4801 gaggaaaagc gaatcaataa tgatttaaat caatatgata caacgctatt taataaagat
     4861 agtaaagaag tcaacgacgc gattgctaag cagaaaaaag aacgacaaca acaaataaaa
     4921 aatgatatgt ttcaaaatca agcgagtcac tcgactcgct tgaatgaaac taaaaaagtg
     4981 ttattttcca aatctaactt agaaaagact tcggagagtg ataaaagccc ctatattcaa
     5041 aacaagcagg agaaaaaaat attcccgtac attttgatgt ctgtaggggc ttttttgact
     5101 ttaggatttg tcattttttc aattcataaa gggagacgaa cgaaaaatga atcagcacgt
     5161 aaaagtaaca tttgatttta ctaattataa ttacggcaca tatgacttag cagtaccagc
     5221 atatttaccg ataaaaaatt taatatcttt agtattggat agtttggaca tttcaatatt
     5281 tgatgtcaat acacaaatta aagtgatgac gaaaggtcaa ttacttgttg aaaatgatcg
     5341 acttattgat tatcaaatcg ctgatggaga tattttggag ttactatagg aggaaaaata
     5401 gatggttaaa aatcataacc ctaaaaatga aatgcaagat atgttaacgc ctttagatgc
     5461 tgaagaagca gctaaaacaa aattacgctt agatatgaga gagattccta agtcttcaat
     5521 taaaccagaa cattttcatt taatgtactt attagaacaa cattctccat attttataga
     5581 tgctgaatta actgaactac gtgatagttt ccaaatacat tatgacatta atgacaatca
     5641 tacacctttt gataatatta aatcatttac taaaaatgaa aaattacgtt acttactcaa
     5701 tatcaaaaat ttagaagaag taaatcgtac acgctacaca tttgtgttgg taccagatga
     5761 attatttttc acaagagatg gattaccaat tgctaaaaca agaggtttac aaaatgttgt
     5821 tgatccatta ccggtgtcag aagctgaatt tttaacaaga tataaagcac tggttatctg
     5881 tgcattcaat gagaaacaat catttgatgc tttagttgaa ggaaacttag aactacataa
     5941 aggaacgcca tttgaaacta aagttattga agctgcaacg ttagatttac taacggcatt
     6001 tttagatgaa cagtatcaga aacaagaaca agattatagt caaaattatg catatgtacg
     6061 caaagtagga cataccgttt tcaaatgggt tgctatcggt atgacaacgt taagtgtgtt
     6121 attaattgca ttcttagcct ttttatattt ttcagtaatg aagcataatg agcgcattga
     6181 aaaaggatac caagcatttg tgaaggaaga ttatacgcaa gtactaaata cgtatgatga
     6241 tttagatgga aaaaaattag ataaagaagc gctttacatt tatgccaaaa gctatattca
     6301 aacaaataaa caaggtttag aaaaagataa gaaagaaaac ttgcttaata acgtgacacc
     6361 aaattcaaac aaagactact tattatattg gatggaatta ggacaaggac atcttgatga
     6421 agcgattaat atagccactt atttagatga taacgatatt acaaagttag cgttgattaa
     6481 taaattaaat gagattaaaa ataacggaga tttatcgaat gataaacgtt ctgaagaaac
     6541 gaaaaagtat aacgataaat tgcaagatat tttagacaaa gaaaaacaag ttaaagatga
     6601 aaaagcgaaa tctgaagaag agaaagcaaa agcaaaagat gagaaattaa agcaacaaga
     6661 agagaacgaa aagaaacaaa aagaacaagc acaaaaagat aaagaaaaac gtcaagaagc
     6721 tgaaagaaaa aaatagtata ggactgaggc aaagacaatg cataaattga ttataaaata
     6781 taacaaacaa ttgaagatgc tcaatttgcg agatggtaag acatatacta ttagcgaaga
     6841 cgagcgtcca gatattacgt tgaaatcgtt aggcgaagtc attcatttag aacaaaataa
     6901 tcaaggtact tggcaagcga atcatacttc tattaataag gtgcttgtta gaaaaggtga
     6961 ccttgatgac attacattac aactttatac agaagctgat tatgcatcat ttgcttatcc
     7021 ttcaattcaa gatacgatga caattgggcc aaatgcgtat gatgatatgg ttattcaaag
     7081 cttgatgaat gccatcatta ttaaagattt tcaatcaata caagaatcac aatacgtacg
     7141 cattgtgcac gataaaaata cagatgtgta tattaactat gaactacaag agcaattaac
     7201 aaacaaagct tacattggtg atcatattta tgttgaaggg atatggctcg aagtacaagc
     7261 tgatggttta aatgtattga gtcagaatac agtggcatcg tcattaattc gcttaacaca
     7321 agagatgcca catgcacagg cagatgatta caatacgtac catcgttcgc caaggattat
     7381 tcaccgtgaa ccgaccgatg atattaagat tgaaagacca ccacagccaa tacagaagaa
     7441 caatacagtg atatggcgtt ccattatacc gccattagta atgattgctt taactgttgt
     7501 catcttttta gtgagaccaa ttggtattta tattttaatg atgattggta tgagtacagt
     7561 aacgatagta tttggtatta caacgtattt ctctgaaaag aaaaagtata acaaagatgt
     7621 tgaaaaacga gagaaagatt acaaagctta tttggataat aaatctaaag aaattaataa
     7681 agcgattaaa gcacaacgtt ttagtttgaa ttatcattat ccaacggttg ctgaaattaa
     7741 agatatcgtt gaaacgaaag caccaagaat atatgaaaaa acatcgcatc atcacgattt
     7801 cttacattat aagttaggta ttgcgaatgt agaaaagtca ttcaaattag attaccaaga
     7861 agaagaattt aaccaacgtc gtgatgaact attcgacgat gctaaagaat tgtatgaatt
     7921 ttacacagat gtagaacaag caccattaat caatgattta aatcatgggc caattgcata
     7981 tattggtgca cgacatctca ttttagaaga attggagaaa atgctaatcc aattgtcaac
     8041 attccatagt tatcatgatt tagagtttct atttgtgaca cgtgaagatg aagttgaaac
     8101 attgaaatgg gcacattggt tgccacatat gacattgaga ggtcaaaaca ttagagggtt
     8161 tgtttacaat caacgaacac gtgaccaaat tttaacgtca atttatagca tgattaaaga
     8221 acgtatccaa gctgtgcgtg aacgcagcag aagtaatgag caaattattt tcacaccgca
     8281 attagtgttt gtcattacag atatgtcatt aattattgat catgtcattt tagaatatgt
     8341 aaaccaagat ttatcagaat atggtatttc attaatcttt gttgaagatg tgattgaaag
     8401 tttgccagag catgtagata ccattattga tatcaagtct cgtactgaag gcgaactgat
     8461 tacgaaagaa aaagaattag ttcaattgaa atttacacct gaaaatattg ataacgtcga
     8521 taaagaatat atcgcgcgac gtttggcgaa tttgatacac gtcgaacatt tgaaaaatgc
     8581 aattcctgat agtattacat ttttagaaat gtataacgtg aaagaagtag atcagcttga
     8641 tgtggttaat cgatggagac aaaatgaaac atacaaaacg atggcagtac ctttaggtgt
     8701 aagaggtaaa gatgacattt tatcattgaa cttacatgaa aaagcacacg gaccacatgg
     8761 tttagttgct ggtaccactg gttcagggaa atctgagatt atccaatcat acattttatc
     8821 tttagctatt aattttcacc cgcatgaagt tgcattccta ttgattgact ataaaggtgg
     8881 aggtatggcg aacttattta aagatttagt ccatttagtt ggtacgatta caaacttaga
     8941 tggcgatgaa gcgatgcgtg ccttaacatc aatcaaagcc gaattgagaa aacgtcaacg
     9001 tttattcgga gagcatgatg ttaaccatat taatcaatat cataagttat ttaaagaagg
     9061 tattgcgaca gaaccaatgc cacatttatt cattatttcc gatgagtttg ccgaattaaa
     9121 atcagaacaa cctgatttta tgaaagaact tgtatcaacg gcacgtattg gacgttcgtt
     9181 aggtattcat ttaatacttg cgacacaaaa accatcgggt gttgttgatg accaaatttg
     9241 gtctaactct aaatttaaat tggcattaaa agtacaagat agacaagaca gtaatgaaat
     9301 tttaaaaaca ccagatgcag cagacattac attaccaggt cgtgcgtatt tacaagttgg
     9361 taataatgaa atttatgaat tattccaatc tgcatggagt ggtgcaacat atgacatcga
     9421 aggcgataaa ttagaagttg aagataagac gatttacatg attaatgact atggtcaact
     9481 tcaagcaatc aacaaagact tgagtggact tgaagatgaa gaaacgaaag aaaaccaaac
     9541 tgagttagaa gcggttatcg atcatatcga atctattaca acacgattag aaattgaaga
     9601 agttaagcgt ccatggctac caccattgcc agaaaatgta tatcaagaag atttagtaga
     9661 aacagatttc agaaaattat ggtcagatga tgcaaaagaa gtggaattaa cattaggact
     9721 taaagacgta ccagaagaac aataccaagg accgatggta ttgcaattga aaaaagctgg
     9781 gcacatcgcg ttaatcggaa gtccaggata tggtagaaca acgttcttac ataacattat
     9841 tttcgatgtt gcaagacacc atcgtcctga tcaagcacac atgtacttgt tcgatttcgg
     9901 taccaatggt ttgatgccag tcacagacat accgcatgtc gctgattact ttacagtaga
     9961 tcaagaagac aagattgcta aggcgatacg gaagatacat gatattattt ctgagcgaaa
    10021 aagactatta agtcaagaga gagtagttaa tatagagcaa tataataaag aaactggaaa
    10081 ttcaattcca aatattttct taattatcga taactacgac acggtgaaag aatcaccgtt
    10141 tatggaagaa tatgaagaaa tgatgtctaa ggtaacacgt gaaggtttag cgttaggggt
    10201 atacatcatt ctttcaggat caagatcaag tgcaataaaa tcagcaatat ttactaacat
    10261 taaaacaaga gttgcgctgt acctctttga aaacaatgaa ttaacaaata ttattggttc
    10321 ttataaaaaa ggtgtgaaag atgttaaagg tagagctgca ataaatgatg ataacttcac
    10381 acagttccaa attgctcaac catttgaatt agcagaagga caaacatata atgagcgcat
    10441 taaaaatgaa gtagcacaaa tgaaagaatt ctatgtagga gattatccaa aacatattcc
    10501 aatgatgcca gataaggtat taatggagga tattcgagaa gcatatgatt tggaaaaaat
    10561 cattcatgaa gaacacaaat taccattagg attagacttt gaggatgtgg aactagttag
    10621 tttagatctg acatcaccaa gtatattaac agcaagaaca ccaaatgatt tagaaatagt
    10681 taataatcga gttttagatg gtatgagtaa acttaaggaa agtgtaacaa caatactagt
    10741 tgatgcagat gaaaatatga gtgataagtc aagtatagtt aattcatatt atgcatcgtc
    10801 agaggatttg caattaataa aacaaggttt tatcgttgaa atcaaaaaaa ggttgaatag
    10861 cgaacgaagt aaagaaagtg tgaagattgt atttatcaac aatcttaagg cgtttatttc
    10921 tatcacagga cttaatgaaa aggatataag gtatatatta tctgaaggac caaaggtaaa
    10981 tgtctataca ataattagta gtatgtacaa tgacatctta ggaacatttg atagagaatc
    11041 aaaattagca cgtcaaatta ttaatcaagc agtaattgta actaggctat atgatcaaga
    11101 gtttttacaa gctaaaatca cgaatagaga gccgttatta aaaccatatg aaatgtatta
    11161 ttttaataaa aatgaacata ttaaaataaa attaattcaa tagggaggac aattatgggg
    11221 gaaataaaag ttgaaacgag tagtacgata ggaaaaattg aaagtatttc taatgctgga
    11281 tcaaatgttt cttttgataa tgtatcagac tcgttagaat acacaaacat ttcaccattt
    11341 acaggatttg ctgctgctag tagtacttta actacagcta tttctaatta tcattctatt
    11401 atcactcaag acacagaagc aatgaaaact gctgtaaatg attttagaga taaggatgct
    11461 gatatagctg gacaaattga tggaaataat aatttaggag caggattata acatagaggg
    11521 agataaaaat ggctaataag gaagagctca aaaaaagaaa ggcacgatgt atagaacaaa
    11581 aacatagcaa ggaaagtgaa ttgaaaagct tacaaggaga taagaaaagg ctgcaagatg
    11641 cagtgaattc tgcacagacc ataatagaag atttcaactc tgaaaagaag acttatgatg
    11701 gtatagaaat tgaaaagaat caatggtctg gacaaacacg taacaaatcg gatgataatc
    11761 gcaccaattt aaatacgtca atagataaat ataaagaaaa gtttgataag gccttaaatc
    11821 aaatgaagaa ggatttggaa aatcttgaaa gtaaaataga aggcgttgaa acggatattt
    11881 caaacattgc tgctgaaatt caaaatatag acagtcaatt ataaatataa aagagtaaag
    11941 gagtataaaa tggggaataa aataaaaatg tcagaagtga ataattttaa tgcttcatta
    12001 cagaaatctc tcagtagctt atcgaccaac acaaaaacct taaagaataa tattaataaa
    12061 ttgaaagatg atagtgaatt taaagggaag acagcatcga atattaatag ttataatcaa
    12121 agttttcata ttgaaacaat aagtagaatc gaaaaaataa aggaggaatt tgaaaaaaaa
    12181 ttaaaagatg caattgaaga ttttcaaaat agtgttgata atgaccataa tgcaatttta
    12241 gatgaagatg caatagaaaa atacaaaggt gaagtcaaaa aatctattga acaaatagag
    12301 agtattaaga caagaatgaa ttctactata ggaggggttc atgatttaac aacagcaaaa
    12361 acaataagtg gtaaggatgt taaagaaaag ggagaaacat ttaataagca aattactgat
    12421 acattagata attttaataa ttttcaaagt ttacactttt tagatgattc cgaattatta
    12481 tctatgatta cacctgtttc tacaatggcc tctaaagtta aaaatatgcc aagtaatcga
    12541 tcaactatat ctggaaatgc tggatatata aaaagtgagt ataaacttca ttcagaaaac
    12601 aaccaatttc gtgaattgaa aaaagaattg gaaaaatatg aaagcgcaat atatggtggt
    12661 aataaatatc gaaagactgt taaatctatg attgaaataa aaaatgcatt aattgttgca
    12721 tatatagctg gtgacggtaa tatctttaaa ggtaatgaat tattagctaa aaaccagttg
    12781 gggaaattgg gaaataaaaa attaaaatta ataaatgcaa ctatgcaaac aaatgttgaa
    12841 aatattgaag gaaaaagatt agttaaagct gctaaattta tacttgataa aaataataaa
    12901 atgaaattca gtgagaaatt aaaagctggt tttaaaatga ccagaaatta tagcgatact
    12961 gaattcaagc agctagaaaa agttgcagga aaatacaatg gtaaatctgc aatgaaaaga
    13021 ggtgctaaag ccgcatggga aagtatgagt caaaaacagt tacgcgaatt tttgaaaaat
    13081 cctaaaaatt ttaaaaatat atgtttggat gaagttaagg attttctagg caaaaacaaa
    13141 ctaggaaaat ttttgaaaag tatgagatat gctggtaagc ttttagggcc actaggagca
    13201 gtagcagcag tagcaaacaa tgttgtaaat gagaaatcag gacagaaaaa aattgttgga
    13261 tctattgttg atttaggggc aattggtggt tcagctgcaa ctggtacggc aatcggtgct
    13321 ggaattggag gaccttttgg tgcagcaata ggtgctgcaa ttggaacaat ggtaggtatt
    13381 tcaacagaca ttaagattgt tggtggcaag tcattgactg atcttgctaa agataaactt
    13441 aataatacgg ttagttctat tagaagtaat ggttttggta aaacgttaag caatgtattt
    13501 taaaaggaga tttaaaatga ataatactaa ggaaaatatt gatactcttc gaaaaccagg
    13561 tgcacaagca ttgagtttag catcattatt tatgatactt ttttcatgtc taactttctt
    13621 ttttggatta gattatgaaa ggtttccaaa ttatttaaag ataacgacaa ttatagaatt
    13681 aataattatc ataattagtt tacttcaatg gattagattt atagatttcg aaaaggaaag
    13741 cgcacagaaa tataaaaaaa tatatgcccg atttttagtt gttataaatg tgctaactac
    13801 tatcaccgca gtatttgcaa catgtaatct ttactatttt gttgctgtac aaaatcatta
    13861 tgacctattc aattattggt tgatgggtac gatttcaatc ataattagtt atttattatt
    13921 agtaattggc ggaatgttca cgttattaaa attacctaaa gtaacaaaac gatggggtgg
    13981 taaaactaaa acacattttg gtttattatt aactgcgttg agcgcattta tatatattga
    14041 aagaattatc gaatatatat tagttcctaa tgtcgtagaa tcgaagtttg tcataatggt
    14101 aagtatcatt attattgctt gt
//
LOCUS       DAHHYJ010000069        14121 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000069 DAHHYJ010000000
VERSION     DAHHYJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.069"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..551
                     /locus_tag="NBK22_001519"
     CDS             <1..551
                     /locus_tag="NBK22_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD2414708.1"
                     /translation="LLENVLPIVPSEIILTFAGLMSVKSHLSILTLFIIATIASFIGL
                     LILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRANDWFKKYGVWAVFICRFIPVLR
                     VLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGRTLSDSFGMLMTGLHTYSRIMY
                     VVIIIAVIYFAIRYIGKRKRVK"
     gene            1070..1765
                     /locus_tag="NBK22_001520"
     CDS             1070..1765
                     /locus_tag="NBK22_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2414709.1"
                     /translation="MDVLTIEHLTKKIGNKTILEDVSFKLKRGQIVGLVGANGAGKTT
                     LMKVILGYSSFQSGNFNVINSKDSKSNIGALIENPGIYPFMSGYENLKLLNESKNTQD
                     IDKIVSQLHMDEYIHKKAKTYSLGMKQKLGIAIAFLNDPQFIILDEPMNGLDPKAVRD
                     VRELIVQKAQEGVTFLISSHILSELVKITNSILIINKGKIVTETSEEELKQFKDNDLE
                     NVLLEIIEREDQA"
     gene            1767..2537
                     /locus_tag="NBK22_001521"
     CDS             1767..2537
                     /locus_tag="NBK22_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414710.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYLATKYNKIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            2911..4875
                     /locus_tag="NBK22_001522"
     CDS             2911..4875
                     /locus_tag="NBK22_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCD2414711.1"
                     /translation="MTQITEKELKKKYLDLLSQNFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKERLSTKELNDLTALVYYPEDKLKL
                     IKSDFQSCGQLNVWYITTIEHLIELIKYCSSKYTRSKLRKALPKQYVYIIEELLYKSN
                     EYQNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIIEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPIIKRRP
                     NFEMEERLVLEKVNYDTNEITVYGNTYPLKDTCFQTINRNNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGHTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRVVDKELQ
                     RKKIRDTNIGKELQAQIDILKMLMHDRYLD"
     gene            5218..6297
                     /locus_tag="NBK22_001523"
     CDS             5218..6297
                     /locus_tag="NBK22_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414712.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGAYSISGI
                     IVTGLLITLGGMIVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(6403..6996)
                     /locus_tag="NBK22_001524"
     CDS             complement(6403..6996)
                     /locus_tag="NBK22_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2414713.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLEFRGQELLAFIKEAAERYEFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAVVEEVWVNGHEITETGLTAGQQILGK"
     gene            complement(7027..7986)
                     /locus_tag="NBK22_001525"
     CDS             complement(7027..7986)
                     /locus_tag="NBK22_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001628197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCD2414714.1"
                     /translation="MINNHELLGIHHVTAMTDDAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPNITKGQAGTNSITRPSFRVPNDDALMYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNDGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIVNRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEIRPFNTKR"
     gene            complement(8064..8498)
                     /locus_tag="NBK22_001526"
     CDS             complement(8064..8498)
                     /locus_tag="NBK22_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2414715.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            8724..9008
                     /locus_tag="NBK22_001527"
     CDS             8724..9008
                     /locus_tag="NBK22_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD2414716.1"
                     /translation="MSNLEIKQGENKFYIGDDENNALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            9179..9985
                     /locus_tag="NBK22_001528"
     CDS             9179..9985
                     /locus_tag="NBK22_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2414717.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFGITVHSNTDLVPEK"
     gene            complement(10106..10777)
                     /locus_tag="NBK22_001529"
     CDS             complement(10106..10777)
                     /locus_tag="NBK22_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD2414718.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            11047..12039
                     /locus_tag="NBK22_001530"
     CDS             11047..12039
                     /locus_tag="NBK22_001530"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD2414719.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLEDDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFDHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
     gene            complement(12286..13086)
                     /locus_tag="NBK22_001531"
     CDS             complement(12286..13086)
                     /locus_tag="NBK22_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD2414720.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKPRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDYLNMNQSIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWGMSSSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFKIASAVAPSNDKQGVLTIIEQEVLNK"
     gene            13427..13591
                     /locus_tag="NBK22_001532"
     CDS             13427..13591
                     /locus_tag="NBK22_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002486687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epipeptide YydF family RiPP"
                     /protein_id="HCD2414721.1"
                     /translation="MNKELQTQKAEKNDLMKDLEIKQLVENSEKLAKVNDLWYFVKSQ
                     ANRWVVGSGH"
     gene            13654..>14121
                     /locus_tag="NBK22_001533"
     CDS             13654..>14121
                     /locus_tag="NBK22_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCD2414722.1"
                     /translation="MYNKSVSINLDSKCNASCDHCCFSCSPFSSVKMEDSYIRTTVLE
                     FAKNSEIDLISFTGGEIFLNYGFLEELLMITKNYNKKVTLISNGFWGSSRKLVRKYFS
                     DFMKYNVVALTISYDEYHEPFVKVNSVKRIFEYSRYYPNVEVSLNMAVTKNKMS"
BASE COUNT         4631 a         2431 c         2190 g         4869 t
ORIGIN      
        1 ttttattaga aaatgtttta cctatcgtac catcggaaat tattctgaca tttgctggcc
       61 ttatgtctgt taaatcacat ttatcaattt taaccttatt tattattgca accatcgcat
      121 cgtttatagg gctgttaatt ttatattata tctgtcgttt aatttcagaa gaacgtctat
      181 atcgctttat tgatcgacac ggtaagtgga ttaaattgaa aagtaaggat ttaaagcgag
      241 caaatgattg gtttaaaaag tatggcgtat gggctgtatt tatctgtcgt ttcatacctg
      301 tattacgtgt attgattacc attccagccg gtgtaaaccg catgaatgtt gtgacattta
      361 ccgttatttc attaataggt acaacaattt ggaatttcgg tttaatttta ctaggacgca
      421 cgttgagcga tagctttggt atgttgatga ctggtcttca tacatattca cgtattatgt
      481 atgttgtcat cattatcgca gtcatttact ttgctatacg ttacattggc aaacgtaaga
      541 gagtaaaata atattgttgt tagtataatg gtatattttc aattgctata aataaaaagc
      601 agtaaaatca tttcgatggc tggaatgatt tactgctttt ttaggtttat ttggcatact
      661 ttagttgaaa gtcatctaaa accctttacg ttaagagata taatgaagtt tcccataaaa
      721 gttcagttcc ctcagtatgc accatttatt ccgcaaactt atctcacaaa tccatctata
      781 atacaaacta tcattttaaa taaacagaat ttaccctgtc ctccacacaa attgttttcg
      841 tcactttaat gtgcctacta tccaaaaaag ttactcataa caaattgcgc attttaataa
      901 atgtagcaat gccatttgca tgctacaaaa atactcgtat tcatatttat gttttatatt
      961 attaaagttt tttaaatcca tatttttata acacttgcta tgtgataaaa ttaattttat
     1021 atataaaatc ttaagattca gattatttaa tagcaaagga gatagtgata tggatgtttt
     1081 aacaatagaa catttaacaa agaagatagg caacaaaacg attctcgaag atgtatcatt
     1141 taagctgaaa cgcggacaaa tagttggtct cgttggagcg aatggtgcag gtaaaacaac
     1201 tttaatgaaa gttatattag gttactctag tttccaaagc gggaatttta atgttattaa
     1261 cagcaaggac agcaaaagca atatcggcgc attgattgaa aatccaggaa tatatccttt
     1321 tatgtctgga tatgaaaact tgaagttatt gaatgaatca aaaaacactc aagatatcga
     1381 taaaattgtc tcacaacttc atatggatga atacattcat aaaaaagcta aaacgtattc
     1441 tcttggtatg aaacaaaaat taggaattgc tatagcattt ttaaatgacc ctcaatttat
     1501 tatcttagat gaaccaatga atggcttaga tccaaaagct gtgcgagatg tacgtgaatt
     1561 gattgtccaa aaagcgcaag aaggtgttac tttcttaatt tcgagtcata ttttaagtga
     1621 attagttaaa atcacaaact ctatccttat tattaacaaa ggtaaaattg ttacagaaac
     1681 atcggaagaa gaacttaaac aatttaaaga taatgattta gaaaatgtat tactagaaat
     1741 catagaaagg gaggaccaag cataaaatgg gaactttaat taaacaagaa tgtttcaaat
     1801 tatttaaaaa gaaatcaact tttatcgcac ctattgtctt tattctacta atggttgctc
     1861 aaggttatct tgctacaaaa tacaataaaa tttttacgcc acaggaatct ttcacatctg
     1921 cttataatgg tttttcatgg tttgcatttt tattaattat tcaagcaagt acaatcattt
     1981 caatggaatt tcattacggt acgattaaaa atttactcta tcgtgaatat tcaagaacaa
     2041 ctatgattgt tagcaaaatc atcacattat ttattatttc tttaatttat tttgttatta
     2101 caattattgc ttcaattgtt attgggtctt tattctttaa tgatttaaat atatttgaaa
     2161 gtagcggtaa tcaattatct ttattgaatc aattattatt agttagttta ggcacatttg
     2221 ttggcgtttg gttagtttta agcttaacgt tgctattatc atctgcaact aattcaacgg
     2281 gagtagccat tgctgtaggt attgtttttt attttgcaag ttctatttta gcagttattc
     2341 aaacggcact tttagaaaaa atagactggc taaagtggaa tcctattaat atgatgaata
     2401 ttatgcttca aacagttgaa aaaggcttta gtaagtcgac gaaattagaa cttcatgaat
     2461 tgtttattgg taatattgct tatatttcta ttttcttaat acttgtagta tttattttca
     2521 agaagaaaaa tatttagtaa cttcaagtta ttaaatgtct aaatacacac atattccatc
     2581 gtaattcaaa atcattttca aatcccttca cccaaataat ggtgcgggga tttttttatc
     2641 caaattttgg caattccaca ttttacatat cgtaattttt taggaaacta gtgaatataa
     2701 caaattcctc ctctcatttt taaaataaat atatcacttt cccactttta cttaactaaa
     2761 ctgcaacgac tcctaatacc aaaatcctgc cctctatttt tatcaatcca agcatactta
     2821 ttgaaaaatg ttaacgtttt cttgataatc attgtaagcg catttatttt ataaactaac
     2881 gtttgaaata tactacagga gtgacacgta atgactcaaa ttactgaaaa agaattaaaa
     2941 aagaagtatt tagatttact atcccaaaat tttgatactc cagaaaaact tgcaactgaa
     3001 attatcaatt tagaatcaat tttagaatta cctaaaggta cggaacattt cgtcagcgat
     3061 ttacatggtg aatacgaagc tttccaacac gtattacgca acggttctgg gaatgtgcga
     3121 gcgaaaatca atgatatttt caaagagaga ctttcaacta aggagcttaa tgatttaact
     3181 gctcttgtct actatccaga agacaaatta aaattgatta aaagtgattt ccaaagttgc
     3241 ggtcaactta atgtctggta tatcacaaca atcgaacatt taattgagtt gattaaatat
     3301 tgttcttcaa aatatacgcg ttcaaaactg cgtaaagcat tgccaaaaca atacgtttat
     3361 attattgagg aactactgta taaaagtaat gaatatcaaa ataaaaaatc atattacgaa
     3421 acacttgtta accaagtaat tgaacttaaa caggcagatg atttaattat tggactcgct
     3481 tattccgtac aacgcttagt cgtcgatcat ttacacgttg tcggtgatat ttatgatcgt
     3541 ggaccacaac cagataaaat tatggataca ctgattaatt atcattccct agatattcaa
     3601 tggggtaatc atgatgtgct ttgggttgga gcctatgctg ggtcaaaagt atgcttagca
     3661 aacttacttc gaatttgtgc acgctatgat aatttagata ttatcgaaga cgcttatggc
     3721 attaatttaa gaccactgct tactttagct gaaaaatact atgacgcaga taatcctgct
     3781 tttaagccta aaaaaagacc tgacaaacat gaacgtttaa ctcaacgtga agaaagtcaa
     3841 attactaaaa ttcatcaagc tattgcgatg attcaattca agttagaaat accaattatt
     3901 aaacgtcgtc caaatttcga aatggaagaa cgtcttgtgc ttgaaaaggt taattatgat
     3961 acaaatgaaa ttacagttta tggtaataca tacccgttga aagacacatg tttccaaact
     4021 atcaatcgta ataatccagc agaattacta cctgaagaag aagaagtcat gaataaacta
     4081 ttattgtcat tccaacaatc tgaaaaatta cgtcgtcata tgtctttctt gatgcgtaaa
     4141 ggttcgcttt acttaccata taatggtaat ttactcattc atggttgtat tccagttgat
     4201 gaaaatggtg agatggaatc atttgaaatt gatggtcata cttacagcgg ccaagaatta
     4261 ttagatgtgt ttgagtatca tgtccgtaaa tcatttgatg aaaaagaaaa tactgatgac
     4321 ttatcgacgg atttagtttg gtatttatgg actgggaaat attcgtcatt atttggtaaa
     4381 cgcgcgatga ctacgtttga gcgatatttt attgcagata aagcttctca taaagaagaa
     4441 aaaaatccat actatcattt acgtgaagat gtgaatatgg ttcgtaaaat gctcagtgat
     4501 ttcggattaa atccagatga aggacgcatt attaatggtc acacaccagt gaaagaaatc
     4561 aatggcgaag atcctatcaa ggctgatgga aagatgcttg tcattgatgg tggtttttca
     4621 aaagcttatc agtcaacaac tggcattgca ggctatacat tgttgtataa ttcattcgga
     4681 atgcaactcg ttgcacacca acaatttaac gctaaagaaa aaatactttc cgaaggtatc
     4741 gatgaacttt ctataaagcg cgttgtagac aaagaattac aacgtaagaa gattcgtgat
     4801 accaatattg gtaaagagct tcaagctcaa atagatattt tgaaaatgtt aatgcatgat
     4861 cgttacttag actaaagacg acatttgatg catgtatcgt gcacttattt agtggtactg
     4921 tgtttacgca ttggctacgc aaacacgatg ataaataggc gacctagcta caccgaaaaa
     4981 atcccctcac tattgcgagt agtgagggga ttggtgtatc tatgctaatt cctgttaact
     5041 gtattataac aactttacag caaaaaaatg caaaatcaac taataaatta atgaaatgac
     5101 cgacaccctc gcttctactc caatttaaca aaatactata ttagtactat atttttcatt
     5161 aacatgtatg ttacattttc tatattactt aaaattacga agggacattt ttaaatcatg
     5221 cgtatcttaa aagagtccat tattgtggca tttgcctttg tgggtgttgt cgttggtgcc
     5281 ggctttgcta ctggtcaaga aattttccag tttttcacaa gtcatggcgc atatagcatt
     5341 tcaggcatta ttgtaacagg actattgatt actttaggtg gaatgattgt catgcataca
     5401 ggtcatcatc taaagtctag aaatcattct gattcaatta actatttctt atacccctct
     5461 attgcaagag gttttgatat tattttgaca atgtttatgt tgtctttagc tattattatg
     5521 actgcaggtg gtgcgtcaac cattcatcaa agtttcaact taccgtattg gctgagcgca
     5581 ctcatattag tcgcctttat tttagcaaca ctgtttctaa aattcgatcg tttaattgct
     5641 gtgcttggcg gtgttacccc atttttaatt gcgattgtca ttatgattgc ggtctactat
     5701 ttcacaacaa gtcatcttga ttttactgcc gctaataatg atgctcagat tcataagcag
     5761 aaatcattat cacctggatg gtggtttgat gcgattaact atgcaagttt gcaaattgct
     5821 gctgccttca gcttcttatc agtgatgggt agcaaagtta aatatcgtga ctcaacgtta
     5881 tatgggggct tgattggcgg tttaatcatt acatttttac tcatgatgat taatctaggt
     5941 ttaatttctc aattcgataa aattaaacac gtcgatttac ctacattaaa attagcgaca
     6001 caaatgtctc cgtcaattgg tattattatg tctgtcatta tgatacttgt catctacaat
     6061 actgttgttg gattaatgta tgcatttgcg tcacgtttca gcgttccatt cagcagacgt
     6121 tacttcatca ttattattac aatggctgtc atcacttata ttagtacatt tatcggtttc
     6181 atttcattaa ttggaaaagt attccctatt atgggattgt tcggtttcat cttactcata
     6241 cctgtactct ataaaggttt aattaagcgt attaccggca aatctcatat cgattaaaat
     6301 atctatacta ttgtaaaatt tagtacaaca cttcaaaatc aaaaaaagcg ggcagatgaa
     6361 aagtaaaaat cttttccatc tgtccgtttc ttaatagaac tgttatttcc caagtatttg
     6421 ttgaccagcc gttaatccag tttctgtaat ttcatgacca ttcacccaaa cttcttcgac
     6481 tactgcccca cgtgtcttaa acaagttaat gacttgttca cttgcagcta atggcacaat
     6541 tggatcatgt ttccccatag aaagcaacac acttacatct gataaatcct ttgttgacgt
     6601 tacttcaact gggtataacg gtgcatataa caatgctttt ttaaatggtg cttccgaacg
     6661 caacattaag ttaatcgcta tatttgatcc atttgaaaat ccaacaagta ctgctttttc
     6721 aatatcaaat tcataacgtt cagcagcttc tttaatgaac gctaacaatt cttgtccacg
     6781 aaattccaaa tcttcttcat cataaacacc ttcaccaaga cgtttgaaat accggttcat
     6841 cccattttct gaaacttgtc ctctaatact taacaagtga taattttcat tcaatgcttc
     6901 gcctaatggc aataaatcga attcatcgcc acctgtacca tgcaacaata ttagcgttgg
     6961 cgcacctttt tgtccttcac gaaaaatatg ttccatcaca aatcctcctc attccaatta
     7021 atcatgttaa cgcttagtat taaatggtct tatttctgat tctatatact ctcttttatt
     7081 ttctaaaaat ggtggtaagg ataacccttc gcctaatgtt tcatacggtt catcttccat
     7141 aaatcctggt ccatctgttg aaatttctat taaaatatgc cccacacgtg catataatgc
     7201 ttcaaaatag aaacgattaa cgatgcctga gttgttaata cctacctctt tatatttcgt
     7261 tgcccacgct tctattgcat catgatcttt cacacgaaat gacacatgat gtacctcacc
     7321 ataaccttga cgtgctgctg gccctttatc atcttttatt aagattacct ggccaccatt
     7381 gcctccttcg ccaacttcaa gtaatgcgac attatcttca tgcgcaatag ttgtcatacc
     7441 gtaaacagtc tctaaaatat ttttaaagtc gtcaaaataa cttactttaa tttcaatggg
     7501 tcctaatcca taaatcgctt tatctactgg aaccggtcca ttcttccaag gtacaccagg
     7561 tgctacccca tcatttaact catctgaaat taattgatac acttggccat cgacttcttc
     7621 aaatggcaac acttttttac caaataattc ttgaatacct tcgtgtttaa caccaaattc
     7681 atcaaagcgc tgttcataat acattaatgc gtcatcgtta ggcactctaa aagacggtct
     7741 tgtaatggaa tttgttcctg cctgcccttt tgtaatattt ggaaaatcaa agaacgtcat
     7801 gtctgtacct gccgaaccta catcatctgc aaaaaatgta tgatacgtat aaatatcatc
     7861 ttgattgact gtctttttaa ctaaacgcat gcctagtact tctgtaaaaa atttataatt
     7921 acgttctgca tcatctgtca ttgctgtgac atggtgaata cctagtaatt catgattatt
     7981 tatcatttta tataccctct ttatattttt agtgtattta tctcgaaatc gagatatttt
     8041 aatgtaaatt tttagtgcac gccttacact tctgtagatt gtgcacttag tttcttaaac
     8101 gcttgttgta agattgttaa ttcatccttt gttaacacat caaacgcttt tgttaatgtc
     8161 tcagcatgct tagggaaaat atctgccatt tgactttgac ctttttcagt taaacaagcc
     8221 atatatacac gtttatcatc tttatccttt tcacgtgtaa tccaaccttt gtcctctaat
     8281 tgacttacaa catatgaaat gctgctactt gcaattaata cgcggtctct aatacgttga
     8341 attggttgcg gacctttatt ataaagcaac tcgagcactg caaattcagt aatatttaag
     8401 ccatatcgct ttacgtcttc ttttgtaatt tgctctaatg tgtctaacgc cctattcatt
     8461 ccgacaaaga cattgagcga ttgtttcgtt cgatccatat cgtcaccaac tttttaattt
     8521 atttcgattt cgagataaat tatatagctg aatagattta atgtcaagat acgtgttttc
     8581 atcttcattt caaactgtgc ttaataaaaa cgaatacata aatgtggcaa tgtcgtcaca
     8641 tgactgtcat gcacctttga cttcgtttaa gttaaaatag aatagggaaa tactataatc
     8701 aatcatgata ggagttgata tgtatgagta accttgaaat caaacaaggc gagaacaaat
     8761 tctatattgg tgatgatgaa aataatgctt tagctgaaat cacataccgt tttgtggata
     8821 ataatgaaat taacattgat catacaggcg tatctgatga acttggtggt caaggtgttg
     8881 gcaaaaaact agttaaagca gttgttgaac atgctcgcga aaatcattta aaaatcattg
     8941 cctcgtgttc atttgccaaa catatgttag aaaaagaaga ttcatatcaa gatgtatatc
     9001 ttggttaaat aaattttgcg atggtgcaac tgtaccatcg cttttttgtt cgcatcaata
     9061 taaaattgca aaacgactag ttattttgca tttcaaactt tatgctatca attcattttc
     9121 ttttctggta tactttttat actaacttat agaaaaatag aaaacgaggc gatgcataat
     9181 ggcatttcac gataaaacag caacacaagt gacaaacatt gaactgaacg taagagactt
     9241 agacttaatg acaacatttt acaaaaacat tttaggacta tccgttaaaa gttctgacga
     9301 taatacaacc gtattatctg ttgggactgg tggccatact ctaacgttac atttattaga
     9361 agacggccgt cagacttccc cacgtgaagc agggcttttt catatagcat ttttattacc
     9421 aactactgag gatctagcta acttcttata tttcgtggca caaaaaaata tgggcatcgg
     9481 cgctggtgat catttagtaa gtgaagcttt atatttcaac gaccccgaag gtaatggtat
     9541 tgaagtctat cgcgatagac cttcatcttc atgggaatgg caaaatggca aagttaaaat
     9601 ggatacttta gaagttgata gccaaacttt attaacacat cgtactgatg aaggttggca
     9661 aggaatgcca gcaaaaggca tgattggaca cttacattta aaaacacatg atttagacgc
     9721 agcatatcaa tgttacattg aacaattagg attccaacat gtgtctgatt ttccacgtgc
     9781 gctatttatg tcaacgaatc attatcacca tcatattgct gctaatactt ggcaatccaa
     9841 tcaagcaaga caaaacaatg cacaaagtta tggattaaca catgttgata tttatcaacc
     9901 cgacgcctca acacatacat ttacggcacc agaagggttt ggcatcacag ttcattcaaa
     9961 tactgacctt gttccagaaa aataatcatc catactttat taatacaatt ttttcacgca
    10021 aaagcccgcg attcatttat atgaacgcag gcttttatta ttgctaactt tttaattata
    10081 tatatttaga catgctgtat tctatttatt cttttggtcc aacccattca ataacatctt
    10141 catatgcttg gcgtgtttta tttttcggtg gatcttgttg tctgtagcca aatgcaacca
    10201 tcactgataa accaaattgc tcagtatcta agataccttt atttgctaaa atgtctgtca
    10261 ctgtatccag actaaaacct tccatcggac atgaatcaat acctaacaat gcggctgtcg
    10321 tcatcatatt gcctaatgcg atatacgttt gtttacttga ccagtcatac aaggcttgat
    10381 cattatcaga aatatggaaa tctgcttgga atgcatcgaa tttttgttca acagctggga
    10441 tcgtttgtgc ctcatatttt ttaatatctc ttaacatatg ttgtacatac ggtgatcttg
    10501 acgttacatt tttacgcgca aaaattagca caaaatggct cgctgtatct aattgttttg
    10561 ctgcgcccca actgtgcgct tttaattcat cacgtaacgc ttgatcttga atcacgacaa
    10621 acttccaagg ttctaaccca agagaacttg gagacaatct acctgactct aatattgttt
    10681 caaaatcttc tttcgaaact ttcttttgtg gatcgaattg cttagtcgca tgtctgaaat
    10741 gaaatgcatc cataattgtt tgattcatat tgctcattga acgtagcctc caatttttat
    10801 atttattact atacaaatta tacttcatgt tttaaaaaga ctaaagtaat ccgtttgcaa
    10861 ttcactataa cttgtgcaca tgttataagt tttctgcaat aaaacacact tttgaatttt
    10921 ggaaaattta gtgttataat atatttgtga tatttttcac aattaaattg tggcgtgatt
    10981 ttaatgtaca taatctttaa tttcactcat ttacttttca atcttttaaa agcgaggttt
    11041 ttaataatga caaaaattat gttctttggt acgcgtgatt atgagaaaga gatggcatta
    11101 aattggggga aaaagaataa tgtcgaagta actacttcta aagagctatt atcaagtgct
    11161 acagtcgatc aattaaaaga ttacgatggc gtaactacaa tgcaatttgg taagttagaa
    11221 gatgacgttt atcctaaatt agaatcttac ggtattaaac aaattgcaca acgtactgct
    11281 ggatttgata tgtatgattt agatttagct aaaaaacaca acattgtgat atctaacgtt
    11341 cctagttatt cacctgaaac aattgcagag tattctgttt ctatcgccct acaattagtg
    11401 cgtcgcttcc cagatattga acgccgtgta caagcacatg attttacttg gcaagcagaa
    11461 atcatgtcta aaccagttaa aaatatgact gttgcaatta tcggtacggg tcgtatcggt
    11521 gctgctactg ctaaaatata tgcaggattt ggtgctacaa ttacagctta tgacgcttat
    11581 cctaataaag atttagactt tttaacttat aaagatagtg ttaaagaagc tattaaagat
    11641 gccgatatta tttctttaca tgttccagcg aacaaggaaa gctatcattt attcgataaa
    11701 gcaatgtttg atcatgtgaa aaaaggtgca attttagtta acgcagcacg tggtgcagtc
    11761 atcaatacac ctgatttaat cgctgcagtg aacgatggta ctttgttagg tgctgcgatt
    11821 gatacttatg aaaatgaagc agcatacttc acaaatgact ggactaataa agacattgac
    11881 gataaaacat tattagagtt aatcgaacat gaaagaattt tagtaacacc acatattgct
    11941 ttcttctctg atgaagcagt acaaaacctt gttgaaggtg gtttaaacgc agcattatct
    12001 gtcattaaca ctggtacatg tgaaacacgt ttaaattaat atatatttaa aagcaaaggc
    12061 gcgccatgtg cctttgcttt ttattttgta tatctgtata aaaaccagtt tgattaaaat
    12121 ccgctttatt ttatcatcta ttcaaatgat tttagtgcgc ttattttact atggcattat
    12181 tgcctaacgt gtttgagata tattaatatt tgattattcg tattcaaagt tttcatttaa
    12241 catgtattta gtatcataat agccgcttca ttgatgatgt actacctact tgttcaatac
    12301 ttcttgttcg ataatcgtta aaacaccctg cttatcatta cttggagcga cagcagaggc
    12361 aatcttgaac aattcttcat catgactatt ttccatcaca taactatgct tagcgaacgc
    12421 aagcatatct ttatcattat tcgcatctcc gaaggccatg agctctgaag aagacattcc
    12481 ccatttatct aacaatcgtt ttaatgcctg ccccttagtc atgtttggca taataatatc
    12541 aatgctatcg tgcccacttg agacaagttt aatatcattg ctgaattgcg ttgctacttc
    12601 ttcgtcaaca tttggatgcg tctcacgatt aatattaaat gctattttga cataatcatc
    12661 ctcaggtaat tgttgtagac tgtcaatttc ttttaactga tgataataaa atctcgtatc
    12721 ttctttaaat gcttcagaag tatgttttaa aatatacgcg cttttcaaac cacagatgac
    12781 gagttgatca atactttggt tcatatttaa gtaatcgacg acctgttgaa acacctgacg
    12841 attaaagctt ttataattat ataactcatt gcctttataa ataactgcac cattttcaga
    12901 aataaaatac atatctctat ccccgaaaat agacttcaac ttcgcatatt gattgccact
    12961 cgcagcaata aatgtaatat ctctatttct aagttcagta aaaatcgctt caaatctcgg
    13021 tttatcgtat gtctttttcg aatctaaaaa cgtcccgtcc atatctaccg caatagcctt
    13081 aaccatgatt atcctcctca ttgctctttt agtcgtcttc attatatcca tctcatgaca
    13141 agcaatgaag tttgtcatac cacaatgaaa ctatattcac attttgaaat tgctttcata
    13201 tatgcaaaac acattacact ggatatatat tcaattcccc aaaaacaaaa aacacttcat
    13261 ctaatataat ataatctaca tttttataat ttacacaaat aaagtaacta gcaaattatt
    13321 cctttccata ctttaggtat tcaaatattc taatatatat aattaattga ttttcaactt
    13381 tttttgataa aataaaatta acttaataaa gaaagaggtg aaaactatga ataaagaatt
    13441 acaaactcaa aaagccgaaa aaaatgattt aatgaaagac ttagaaatta aacagttagt
    13501 cgaaaactct gaaaaattag ccaaagtaaa cgacttatgg tattttgtca aatcacaagc
    13561 taatcgatgg gtagttggta gtggtcatta gttttaaata aacggagagt ttcctctccg
    13621 tttatttcat ttttataaaa ggggataatc attttgtaca ataaaagtgt atctataaat
    13681 ttagactcaa aatgcaatgc ttcatgtgat cattgttgtt tttcttgctc accattttct
    13741 tcggttaaaa tggaagactc ttatatcaga acaactgttt tagagtttgc aaaaaattct
    13801 gaaatagatt taatttcatt tacaggtggt gaaatctttc taaattatgg ttttttagaa
    13861 gaactactaa tgattacaaa aaactataat aaaaaggtaa cacttatttc aaacggattt
    13921 tggggcagta gtagaaaatt agttcgaaaa tacttttcag attttatgaa atataatgta
    13981 gtggcattaa caattagtta tgatgaatat catgaaccct ttgttaaagt aaatagtgta
    14041 aaaagaattt ttgaatatag caggtattat cctaacgttg aagtttcatt gaatatggca
    14101 gtaacaaaaa ataaaatgtc a
//
LOCUS       DAHHYJ010000070        13938 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000070 DAHHYJ010000000
VERSION     DAHHYJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13938
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.070"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..515)
                     /locus_tag="NBK22_001534"
     CDS             complement(<1..515)
                     /locus_tag="NBK22_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414723.1"
                     /translation="MNNKLIYTSYDGDNIPLIDSFIKLVIDFKYVPINPTKSLGYYIS
                     TSIHDNDKGECLKDCLSLEMICDELWVFIDNNKYIPEGVRLEIATWLKYKSSPVKYIS
                     IPSLLENSSINDDLFLDFDDSNILKEKEISELVPKKSELRPVNCINILPEHHKYIDWI
                     KYHLFYNKFVP"
     gene            complement(679..1437)
                     /locus_tag="NBK22_001535"
     CDS             complement(679..1437)
                     /locus_tag="NBK22_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD2414724.1"
                     /translation="MSEFKTVKINKHVLYSNILNRDVTLSIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDDK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFDGGHQWKDWKPLLSDILLYFLSKNTDDQLYE"
     gene            complement(1582..3150)
                     /locus_tag="NBK22_001536"
     CDS             complement(1582..3150)
                     /locus_tag="NBK22_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCD2414725.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGAIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            3492..4577
                     /locus_tag="NBK22_001537"
     CDS             3492..4577
                     /locus_tag="NBK22_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD2414726.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(4775..5545)
                     /gene="fabI"
                     /locus_tag="NBK22_001538"
     CDS             complement(4775..5545)
                     /gene="fabI"
                     /locus_tag="NBK22_001538"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCD2414727.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(5823..7667)
                     /locus_tag="NBK22_001539"
     CDS             complement(5823..7667)
                     /locus_tag="NBK22_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCD2414728.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIEVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(7677..9062)
                     /gene="mgtE"
                     /locus_tag="NBK22_001540"
     CDS             complement(7677..9062)
                     /gene="mgtE"
                     /locus_tag="NBK22_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCD2414729.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(9083..9937)
                     /locus_tag="NBK22_001541"
     CDS             complement(9083..9937)
                     /locus_tag="NBK22_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD2414730.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMTNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(9934..10743)
                     /locus_tag="NBK22_001542"
     CDS             complement(9934..10743)
                     /locus_tag="NBK22_001542"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCD2414731.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(10760..11395)
                     /locus_tag="NBK22_001543"
     CDS             complement(10760..11395)
                     /locus_tag="NBK22_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD2414732.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(11412..11759)
                     /locus_tag="NBK22_001544"
     CDS             complement(11412..11759)
                     /locus_tag="NBK22_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414733.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            11945..12538
                     /locus_tag="NBK22_001545"
     CDS             11945..12538
                     /locus_tag="NBK22_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCD2414734.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTADYELEFEVKDYNQG
                     LQKFQSLLNELNLEHHQPLNKVQRFFKKKETLSNNIN"
     gene            12642..13007
                     /locus_tag="NBK22_001546"
     CDS             12642..13007
                     /locus_tag="NBK22_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCD2414735.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            13030..13836
                     /gene="yjbH"
                     /locus_tag="NBK22_001547"
     CDS             13030..13836
                     /gene="yjbH"
                     /locus_tag="NBK22_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCD2414736.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKIEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKIKSK"
BASE COUNT         4781 a         2510 c         1933 g         4714 t
ORIGIN      
        1 aatggtacga atttgttata aaataaatga tactttatcc agtctatata tttatggtgt
       61 tccggtagta tattaataca atttacaggt cttaactcac tttttttcgg tactagttcg
      121 gaaatttcct tttctttaag tatatttgaa tcatcaaagt ctaaaaataa atcgtcattt
      181 atactcgaat tctctaataa agaaggtatg cttatatact taactggaga ggatttatat
      241 tttaaccatg tagctatttc taaccgaact ccttctggaa tatatttatt attgtctatg
      301 aatacccaaa gttcatcaca aatcatttcc aaagacaagc agtcttttaa acactcacct
      361 ttgtcattgt catgaatcga tgtagatata taatatccta atgatttagt tggatttatt
      421 ggaacatatt taaaatcaat aacaagttta ataaaactat ctatcaatgg tatgttatcg
      481 ccatcataag aggtatatat aagcttatta ttcactgtaa atcccccctt cactattcta
      541 tacatttcac cttcttgaag ctctttatcc gttgttattg caggaatatg cctttctctt
      601 aatataatac ctaagtgccc taaaattgcc ctcttttgaa gataaaacca ctacaataat
      661 cagttatata agaacatatt attcataaag ttgatcatct gtgtttttac ttaaaaaata
      721 caataatata tctgacagca atggtttcca atctttccat tggtgaccac catcaaattc
      781 atcgtaataa taagttatat tatatttctt aatttgttca gctaattctc tattcggtgt
      841 caagaaatcg gcacgcttac catttgttgg caaagtgaaa tctttttcat ctagaccaat
      901 gacatgccaa attgtcaatt gttctttatt agcacattga tttagcttat ctaatacttt
      961 gtcatctgaa tgtggactta acattgctac acgactaaaa atcgttggat aggtcaacgc
     1021 cgttaataag gcaatactac ccgctaaact atcccctact aataaccttg cattacctac
     1081 tttcagtgta gaaaacgtcg agtcaataaa tggcaatatt tctttaccga ctgattgaat
     1141 agttaaatga gaacgacttc cttgtggatg aaattcctcc ctacgcttat caacgtcttc
     1201 ataatggaat ccgacaatga tcgcatcatc aatacgcgct tctttgatta acgattcata
     1261 tgtacgttgt attctcccga aacgcaaaaa atctaatccg tcaaagcaaa gaatgacatt
     1321 atatttaaca agttgattat aagattctgg taaataaata cttaacgtga catctctatt
     1381 taaaatatta ctatataaaa catgtttatt aatcttaaca gttttaaatt ctgacataaa
     1441 aaagtgcccc ctagtagttg tattgttatt gtaccaagag acactatatt tttaaaattc
     1501 aaatttctga gcacctaaaa ttatcaatgc tgtttctttt tttatcaaat gatcactaca
     1561 tatactataa acaaaagtag attacttttt ggcacgtgct tgttttaaac gttctggata
     1621 tgtcttcaac caaaagacag cattaggtaa tttattcgga tcaggttgat aaactgcatt
     1681 ataaccgttg cctaaacgtt tcttttgaat ttcataatct tttaaagcat ttacggcagg
     1741 cttatgtaaa atccaaatcg caatgatatt taaccaagcc attagaccta cacctaaatc
     1801 accgaatgcc catgctacat cagctgtttt aactgcaccg taaaatgtag cgaacaaaat
     1861 aatcacacga gcaatattaa taaatatcga tgaaacttga ttattttgat tacgcgttaa
     1921 ataagcaaca tttgtttctg taatgtagta gtacgacaaa atagtagtaa atgcaaagaa
     1981 gaataaagca aatgcaataa agtacgaacc tacgccagag aaagtaggat caaattgata
     2041 accactgcca tggaacgctt tatcaatgcc ggcttgtgca tacatcgcag tacctgaata
     2101 atctttacct gttgcatttt caacataaat accgccatct ttaattaaat gcggtgtgcc
     2161 attcgcatta accgtaccat cagtcacatt atatgtacca gaaataagta taatcagagc
     2221 agttgcagta catacaaata atgtatcaat ataaactgaa aatgcttgta ctagaccttg
     2281 tttacttgga tgtgatactt ctgctgccgc tgctgcgtgt ggacctgtac cttgaccagc
     2341 ctcatttgag tataatccac gtttaacacc aatctctatc attgcgccaa cgataccacc
     2401 aaaagcagat tgtaatccaa atgctgattt gaaaattaat gcaaataacg ctggcacttc
     2461 ttgtatattg atacaaatga taatgacagc catcagtatg taaataattg ccataaatgg
     2521 tacaacggct gttgcaacat tggcaatact acgtacacca ccaaaaataa ttaatcccaa
     2581 aataactaca acaatagcac ccattaacca ttgtggaaca tgaatcgcat tatgcataga
     2641 acttgctata gcgttagatt gcacaccagg aagcaataga cctactgaga taatcgtaac
     2701 aatagcaaaa ataattccgt aaattttacc aaatttacca ccaataccat attcaatata
     2761 atacgctggt ccaccacggt attcattatt ttcaactctc ttgaatattt gaccaagtgt
     2821 agattcaata aaagcactac ttgcacctaa aaacgcagta atccacatcc aaaatactgc
     2881 accaggtcct cctataaata ttgcagtaga tacaccgaca atattacctg taccaaccct
     2941 gcctgctaaa gacatggcta tcgcttgaaa acttgaaata ccattaggag acttctctcc
     3001 ttgaaacatt aatcgaatca tttctttaaa atgtctaact tgaaaaaatc gcatacgcaa
     3061 tgtaaatagc acaccagtaa ttagcaaacc ataaactaat ggcttactcc aaacaacctc
     3121 atttaaaaag gttactaatt tttcaatcat aagattcccc ctattgttta aacataaaaa
     3181 tattatacga ttaagcacga gacacttcaa tatatttttc agaatattct tataaagaat
     3241 aatatgatca tttcattatt taaacacgtt taatattaaa ataagttatt catataaact
     3301 ggtgctgttt gatccaattg cagacttacg agtcattgaa aagtctcaca aactattgaa
     3361 agtaaatatc ttaaaataga aagtgaatgg ttaatttaag tatatttaaa aaatattaac
     3421 ctttttaagc actgctattt aggatatact aaataataac taagtttaga aaaataggag
     3481 gaacatcgtt tatgttaaac aaggtttggt tccgaactgg catcgctctg attatgctgt
     3541 tcattctcat caaactattt atggaagtgc acgaagtatt tactccaata gctacaatca
     3601 tcggttctgt atttcttcca tttttaatta gtggtttttt attttatatc tgtctacctt
     3661 ttcaaaactt acttgaaaaa gtcggttttc caagatgggc tagtataaca acaatcatgt
     3721 tagctttgtt tgctattatc ggcttaattg ttgcgtttgt tgcaccaatc atcatttcaa
     3781 atatcaacaa tttaattagt cagacacctg ccctacaaaa agaagcagag caaattatta
     3841 aattcgcact agctcaaatg gataaattac ctgaagatgt aacaagtaga attacaaata
     3901 tggttaaatc aatgggtgat ggcgttacta atattttatc taattcatta cagtacatta
     3961 catcattaat ttcaaccatc ttcttattga ttatggttcc attcttctta atttatatgt
     4021 taaaagacca tgaaaaattt atcccagctg ttgctaaatt tttcaaaggt gaacgtaaag
     4081 tatttttcgt agatttatta actgatttaa actttacatt aaaatcttac attcaaggtc
     4141 aagtaactgt cagtgttatt ttaggtatat tcttatatat cggttattca attattgact
     4201 taccatatat tcctttatta gtactatttg ctggtgtcgc aaacttaatt cctttcttag
     4261 gttcatggtt atcatttgca cctgcagcaa tactcggtat tatagatagt cctactacat
     4321 ttatatgggt ttgtatcatt acattaattg cacaccaact tgaaggtaat atcattacac
     4381 caaatgtaat gggtaaatct ttaagtattc atcctttaac aattatcgtt gttattttag
     4441 cagcaggaga tttaggtggc tttacattag ttctgattgc agtgccatta tatgctgtac
     4501 ttaaaacggt tgttagtaat attttcaaat accgccaacg cattattgat aaagcaaaca
     4561 gtaatgttaa ggactaattc tgtggatgtc ttttaagaat ataagatact atctcctcaa
     4621 aagttgaaac tacagctttt gaggagattt ttttgtgcat aaaaaaaatc agtcagatga
     4681 aatatcaaat aattttccat cagtccgatt attataaaac caaaaaaagc tttgctcaca
     4741 tatataataa cgtgaacaaa gctattgaat gatattattt aattgcgtgg aatccgctat
     4801 ctacatgaat attttcacct gtaacgccac ttgataagtc acttaataag taagccgctg
     4861 ttttacctac ttctacttga tcaacgttac gttttaaagg tgcacgctct tcgatttctt
     4921 taagaattgt attgaaacca cccacacctt ttgcacttaa tgtacggatt ggaccagctg
     4981 aaattgcatt aacgcgaata ttatctggac ctaagtctaa tgctaaatat ttaacatttg
     5041 cttctaagct cgctttagca acacccatca cattataatt ttgaactgcg aattcgccac
     5101 ctaaatatgt tgttgcaaca atgctaccac cttctggcat taatttttta gcttcatgag
     5161 ccacaattgt taatgagtaa gaactaatgt cttgagctaa caagaagcct tcacgtgaag
     5221 tttcagaaaa gcgtccgcgt aagtcttcca tattagcaaa tgcgattgaa tgatatacac
     5281 catcaatatt gccaacatct ttaccaattt gctcaaaacc attaataacc tcttcatcgc
     5341 tttgaacatc aatttgatat aagtgcgctt ctggttgatt taattgttct aataattttt
     5401 caagctcttt acggctacgt tctttacggt aagtaaatac taatttagca cctaattgat
     5461 ctaaaacttt agcgacacca aaagcaatac tacgcttatt agcgattccc atgatgacat
     5521 atgttttgtt ttcaagattt aacatgtaag ataactcctt ataataatta atttaacgga
     5581 tagaatatct aatcccagtt ttaactaact ttttattcgt caatacattt taacactatt
     5641 atgacttttt aataaagtta tttaaacgta ttacaacgat aaatgtttga tttagtcctt
     5701 tctgtatcaa acttgattta cgtaaaaagc atttatttat caaaatcata tacatactag
     5761 cattttaaaa tcattaattt tacgtcgtac taaaatacgt ctgcatttta atatcattat
     5821 tgttaaaaat aaaattctaa ctcttgcttc aattcatcaa cgtattcttt agcaccggta
     5881 acaattaagc gatctccata tcgcaattgt gtatctccat gcggaacaat cgactcatta
     5941 ttacggataa tacgcacgaa gatgatgtct cctccgaatg ggaaattacg taattgaata
     6001 ttttcatatt tataatttaa catttgaatt tcatatagtg acgtttcaac attacttaat
     6061 aaattcaaca tgttaggtgt ttcaattaaa ccttttaata agattttatt acttaagtag
     6121 ctactgaaaa tttcaatacc tgaatcaact aactctgtat cgtccgttgt gctttcaagt
     6181 ctgcaaatga cacgctcaac ttgatgtgct ttggctaatt tagcaacttt tcggttaata
     6241 tcatcgtcat tcgtggcaca aacaactatg tctcggtcaa acagacctag tcgttctaaa
     6301 acatcttgtt cataatcagc aatttctatc atcgtgatat catccgacaa ttgacgacga
     6361 tcactcaagt ctttgcgata atataatgtc acgtcatata actgagatgt taaattttgc
     6421 gctataggaa tcgttaattg atttttacca attaaactaa cttcaatttt acggttaaac
     6481 tcatctggaa ctggaaacag ttttttgaaa ataatcggaa cgaatacaca tgtaatgact
     6541 gcgcttaaaa ttaatatacc tgacgtttca gctgaaatag catttaatct ttctgaaatt
     6601 ttggctgcag ctatcacgag cgataatgtt gatgttaata aaaatgctga tgcaatcgtt
     6661 gttttcatat caaaccaacg tcgaataaac atgactggaa ttaattttga aatgataaat
     6721 gcaacgatta aaatcggtat gataattagt aatttcggtt ctttaattaa tgaaggtatg
     6781 tttaaatcta caccaaccat tataaagaaa ataggaataa agaacccata accaaatgag
     6841 tctaattttt caaccatttc ttcatctgga tttaataatg aaacaacgac acctgctaag
     6901 aatgcaccta atatattttc tgcgccaact ccctctgcta gggctactaa taatattatt
     6961 aatgcaaaca ccgcacgaat accgatttgc gtcgtaccat ccatcaattt ttgtaaaaat
     7021 gacattcttt taaattgaac acctaaaata tatgaaattg ctgtgaaaac aactaatata
     7081 cctattaacc atattgtact gccgccttga ccattgattg cgccatagac cgttaataaa
     7141 atcatagtta ctaagtccgc aagtactgct actaatagga taaattgccc tatagttgtt
     7201 ctcataatat tcatttcttt taaagttgga acaactacgc ctaaggaaat agttgaaatg
     7261 ataatgacca ttaataacac atcatccact aatccaagcc atttaaatac atacgctaaa
     7321 agaatcgaaa taatcataat aaatgcaaat acagttaacg ctagattaag atgccctgga
     7381 attgaggatt catcatcatt ttgtccttga cgtgcgcgtt tatctttttt aaaagcttta
     7441 aaatcaattt ctaaaccact taaaaacatt aaaaagataa agcctaacgt tgataaaata
     7501 tttagaattg aatccctttc tactatattt agaaatgaat ttccaatcac aatccccatc
     7561 aaaatttctg caacaacaac tggcaagaaa ttaatattta atcgattgac aattattgga
     7621 gttaaaaacg ctgctaaaac aacaataact aaagataaaa actccataac tcctccttaa
     7681 attaagtaag ccataaatga tgtagctaaa ccaaaataaa tcaacatact aataatatca
     7741 ttaatcgttg taataaatgg tccactagcc actgctggat cgatatttaa tttattcatc
     7801 aataatggaa tcatcgaacc tacaaacgtc cccaccgtca tcgcacaagt taaacttcct
     7861 gcaacgatta atgctaaaag tggctgatga tatattgcaa caataattgt aaataatatt
     7921 gttgaacata caacacccga taatacacca cttcccgctt ctcttaatgc aattctaaat
     7981 ttactttgct cattaatttc ccctgtcgta atgttacgaa ctgaaacggc taaagattgt
     8041 gtacctgaat ttcctgacat accactaata ataggaataa acgctgcgag tagcgctaca
     8101 ttttctaatg ttttttcaaa tctccctaaa attgtcgcag taatcattcc taaaaatgtt
     8161 aaaataatca accatggtaa acgttttaat gctgttttaa tgattgaatc attagtcgaa
     8221 tcgatatccg ataccccggc taaacgagag tagtcttcac tagcctcttc atccataacg
     8281 tctaaaatat catcaatcgt gatgatacca agcaaatgtt cttggtaatc tataacaggt
     8341 acagccatga aatcatagtc tctcataact tgagcaacat cttcttggtc gtctgctaca
     8401 ttcacactaa tgacacgttc attcataata tcttcaatat aagcatcatt ttcagctaca
     8461 attaaatctc ttagtgataa aacacctact aatttaccat catcatcaac gacaaatata
     8521 acatatattg tttctgcgtc tggcgcttgc gctttgacca ataataacgc ttctttaaca
     8581 ggcgtatgcg ctttaagtga taaatactcc gtcgtcataa taccgccggc cgtatcctca
     8641 tcatagtgaa gtaatgcttt gatttcattc gcgtcatctt tattcatcaa tgttaaaaga
     8701 ctagcaactt ttggtttagt caactcattt aaaatatcta ctgcattgtc gtatgacatt
     8761 tcttctaata tgtgacttgc gtatgtcgca ttcatcttat caaatagcaa ctcatattca
     8821 tcgtcatcaa tatctaattg atcaaagaaa tttgcaactt cttcaggtga taaatattga
     8881 aaaatctttt gtctattttc atcggtagta tcttcaaaat actcactttg ttcatatgtg
     8941 tgtaatgcta gaaattcatc tctaaattga tcaatatcat cattttctaa atattgatct
     9001 aataaagttt ggtcgtataa ttcctcttga acacgctctt tttcatctgt gttcattgac
     9061 atacttacac cccctgtgtg aattagagtt gattgaataa tttaagtaat tgcttataat
     9121 caattgtaat ataaatattg ttcttattga ttggatgaac aaaatatatt tgcgtacatt
     9181 gcagtacttg accatgaatt ttgtcatgaa aaccatcata caaagaatct cccacaattg
     9241 gatgcccaat atgttgaaaa tgtacacgaa tttgatgtgt acgtcccgta tgcaaatgaa
     9301 ctttgcataa actgtattta tcattctgat ttattacttc ataagatgtt ttagcgtatt
     9361 taccatccga ggcaacttct ctagttataa tactatcctt tgaccgtcta atattagctt
     9421 caataatacc agatgtatgg gttttaccat atacaaggca agtatatatt tttttcaagt
     9481 ttactttaga aaataaatga tggatatgtc catatttagc gaatatcaca ataccagttg
     9541 tattacgatc tagacgcgta acaatatgtg ggttaatatt ttcaccatgt tcctgacaat
     9601 gatatagtac ttgttcgatt aaactttcat gaggatgttc tctcgaaggc gtacaatttt
     9661 gattgtttgg tttagtaact atgatgataa aagcatcttc atacaatact tctagcttac
     9721 gagcataagg tattaaatta acactcggta tttctcgcgg taaatgaatt tctaatatat
     9781 catttgtcat taattgctta cgcactgtga ctggttcatc attaacaatt aaagcgccat
     9841 tatttttaat ggcgctcact gtcttcttag aaaaatcatg tcgtgctaaa aaagttttaa
     9901 cagtttcttg ttgtgatata tgatacttaa atttcatcgt tcttcatcac ttgatatgaa
     9961 agaatcgtgc acacgtttcc agaatgggaa tggtctaaaa cgtgcaaacc tcactttttc
    10021 atttgctaca cggtattgta ttgaattaac atttttatgt ttgatactaa catgatctat
    10081 cgtcattcta atggtatcat gattaactgg tgatattaaa catgtatgat gctttggtaa
    10141 tacaagtggt gatcctaccg ttctaaacac acgattattt atcgaggcaa tttctgtaat
    10201 ttgcattgct tcaagtgaag gatgtatcag tgcgccacct agcgctttgt tataagccgt
    10261 tgaacccgaa ggtgttgata cacataatcc atcgcctcta aatcgctcaa agtgtttccc
    10321 tcttaagtta acatccacaa caagtgttga gccattttca gttttcatcg ttgcttcatt
    10381 taatgctaaa taccttgttt catagccgtt gtcgttgtat ctcataataa tttcaagcaa
    10441 tggatattca atgacctgaa actctgaatt attaatttcg atgattaatt tttcaacttc
    10501 atgaggtaac caatccgcat aaaatcctaa atgacctgta tgaactccaa caaatgccac
    10561 ttttgataac atgtggctat actgatggaa tgcttgtagt aatgtaccgt caccaccaac
    10621 tgaaataaca atttcaggat tttcactatc ctcaatcatg cgaaaatctt tcatatagtt
    10681 catcatttta tgctttaagg cattagactt ggagtcacct ttagttaaaa ttgtataacg
    10741 catgatttat aacacctcgt taatcatttt catgtttttt agaacgtttt tgcgtgtaat
    10801 atttttgagc ttcctgaatt tcatctttaa tttcagacat ctcttcatca agtaaatacg
    10861 ctgcttctgc cgctctttcc aaacgatgtt gaatttcatc cggataagca ccatcatatt
    10921 tatatcgtaa agtatgttca atcgttgccc agaaattcat tgctaatgta cgaatctgaa
    10981 tctcagccaa tataaatttt tggccttgta atgtttcaat tggatattca ataatgacat
    11041 gatacgagcg gtaaccactt tctttagtgt tacgaatata atctcgttct tcaattactt
    11101 taaaatcttt tctttgtctt aaaatattga cgacaacatc aatatcttca acaaattggc
    11161 acatcattct taaaccagcg atatcgtaca tttcttctct taacctatca aatggtattt
    11221 gtcgtttgtt tgccttatct ataatactag cgattggttt aacacgacca gtaacaaatt
    11281 ctattggcga cgcttgttca ccaacttcat attgtttgcg catgccttta agtttcactt
    11341 tcaactcatc aaccgcttgc ttataaggtg ttaaaaactg atcccattga ttcatagtgc
    11401 ttcaccccgc tttattcaat gtcgaatgtt tcttcaacgg ttgcaacaaa ttccttacca
    11461 taattattat tcccttttat gttgtctaaa atataagtaa gttcttggtg gaattcagtc
    11521 aattctagtt catcattgat aataattttt ttagtcatgt tttcatgcaa catcgtccat
    11581 gtttcttgta cgaaaatgag ataagcatac gctttaccat catctaatgt atacgcaaag
    11641 gcataattat cactatcaac gagcatttgt cctacttcag ataatccttt aatagaatct
    11701 tctgtataac aataaagtat gtcatcttta attttaattt cattaatata taaacgcatg
    11761 cttaatcctc ctcttattcc ataacatttt aacacaattc aataatacaa aacacttacg
    11821 aacactattt ttaatggtca aattaacatt gataatagat aaaggaaatt acttttaaaa
    11881 ttcaactttg tcattcataa tatagttaag ataaattgaa tttgatacga ggtgtataca
    11941 tatcatggca acaaatcatg aaatagaatt taaacaaatg ataactgcat caatctataa
    12001 taaattgcaa gagaaatatt ttaaagacag tgtgcttttt aagcaagtca attactatat
    12061 tgatacaccc gatttcaaat taaaggaaca tcgctctgca ctacgcataa gagttaaaga
    12121 caatcaatat gaaatgactt taaagacacc agcaaaggtt ggtctattag aatacaacta
    12181 tatcgtcgac attaaaccag agatgaactt gattatttca aacgacaatc ttccagatga
    12241 cattcgtcaa attatagttg aacagtttgg tgtaaaagat acaactttat caatacttgg
    12301 tgcattaacg acatatcgcc aagaaactaa atataaaggt gatttactcg ttttagataa
    12361 aagtgagtat ttcgatactg cggattatga gttagagttc gaagttaaag actataatca
    12421 aggtttacaa aagttccaat ctttattaaa cgaactaaac ctcgaacatc atcaaccttt
    12481 aaataaagtt caacgttttt tcaagaaaaa agaaacactt tcaaacaata taaattaaat
    12541 tggcaaatat gcatatttct ttcaaatgtt taaaatcatg ttatattagt tatatattaa
    12601 cttaatttcg taatagtaaa tttttgaaaa tgggtgatta aatgacaaca acaccatatg
    12661 acatcattgg taaagaagcg ttatacgata tgattgatta tttttacacc cttgtagaaa
    12721 aagatgaacg acttaatcac ctgtttccag gagattttgc agaaacaagt cgtaaacaaa
    12781 aacaattttt aacacagttt ttgggcggtc caaacattta taccgaagaa cacggacatc
    12841 ctatgctaag aaaaagacat atggatttta caatcactga atttgaaaga gatgcatggt
    12901 tagaaaatat gcagacagca attaatcgcg ctgcgtttcc acaaggtgtt ggagattatt
    12961 tatttgagcg cttaagatta actgctaatc atatggtgaa ttcctaaaat taattgtagg
    13021 tgaataaaca tggctggaga attacgaata atggaaaata agagtcgtga agatattaat
    13081 ctatcacctg taagtaaaat cgaaatttat tcatttttcg atccatttag ctccgattgc
    13141 ttcaaattat cagcaatctt atccaaatta agaattgaat ataatcaata tatacgtatc
    13201 agacatatat taaatccttc gttaaaagta ttaacgaaat gccaagctca aagtacatcc
    13261 aactttgata acatcgccct agcttataaa gcagctgagt tacaaggtcg tgtacgtgcc
    13321 gaacgattta tacatttaat gcaaaatgaa atcataccta aaagagatat tattacagaa
    13381 tcaatgattt gtgactgtat tcaaaatgca ggtattgatt tagaagtatt taaagacgac
    13441 ttacaaaaaa gtaaactaac cgaaagcttg aaaattgatt tgcatattgc aagagaaatg
    13501 gaaatcgaac aagccccttc tctcgttttc tttagtgaag atgttcatga agaaggttta
    13561 aaaatcgaag gattataccc atatcacatc tatacttata taattaatga attgatgggt
    13621 aaacctatcg aaaagaatct tcctcctaaa ttagaaactt atatacagca acaacaactt
    13681 gtaacgatgg aagaattact tactatttat gaatggccag aaaaactttt aaacaaagag
    13741 ttaaagaagt tagccattca acaaaaaatt gaaaaactta aatatccaga tggcgatttc
    13801 tggaaatcta aaatgcctaa aatcaaatca aaataaataa attaaagacc tcgtacacca
    13861 taaatgtaac gaggtctttt tactgattct taattttgca acattaaatt attagaacgt
    13921 aatgttacac atcgatct
//
LOCUS       DAHHYJ010000071        13774 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000071 DAHHYJ010000000
VERSION     DAHHYJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.071"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(248..3094)
                     /gene="uvrA"
                     /locus_tag="NBK22_001548"
     CDS             complement(248..3094)
                     /gene="uvrA"
                     /locus_tag="NBK22_001548"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCD2414737.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIEVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            complement(3102..5087)
                     /gene="uvrB"
                     /locus_tag="NBK22_001549"
     CDS             complement(3102..5087)
                     /gene="uvrB"
                     /locus_tag="NBK22_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCD2414738.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            complement(5356..5592)
                     /locus_tag="NBK22_001550"
     CDS             complement(5356..5592)
                     /locus_tag="NBK22_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCD2414739.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            complement(5589..6239)
                     /locus_tag="NBK22_001551"
     CDS             complement(5589..6239)
                     /locus_tag="NBK22_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD2414740.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            complement(6413..7252)
                     /locus_tag="NBK22_001552"
     CDS             complement(6413..7252)
                     /locus_tag="NBK22_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD2414741.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKTPVRKASQAKKVVKQPVQQASKKVVVKQAPKQAVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            complement(7400..7507)
                     /locus_tag="NBK22_001553"
     CDS             complement(7400..7507)
                     /locus_tag="NBK22_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414742.1"
                     /translation="MCLLKQHMQCAFSNITYDDFQGIELIIENVLNYMV"
     gene            complement(7661..8771)
                     /gene="prfB"
                     /locus_tag="NBK22_001554"
     CDS             complement(join(7661..8698,8700..8771))
                     /gene="prfB"
                     /locus_tag="NBK22_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCD2414743.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     IYFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            complement(8946..9044)
                     /locus_tag="NBK22_001555"
     CDS             complement(8946..9044)
                     /locus_tag="NBK22_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414744.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKK"
     gene            complement(9086..11617)
                     /gene="secA"
                     /locus_tag="NBK22_001556"
     CDS             complement(9086..11617)
                     /gene="secA"
                     /locus_tag="NBK22_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCD2414745.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKYILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            complement(12031..12603)
                     /gene="raiA"
                     /locus_tag="NBK22_001557"
     CDS             complement(12031..12603)
                     /gene="raiA"
                     /locus_tag="NBK22_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCD2414746.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            complement(12664..13338)
                     /locus_tag="NBK22_001558"
     CDS             complement(12664..13338)
                     /locus_tag="NBK22_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCD2414747.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKHLNQDEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            complement(13331..>13774)
                     /locus_tag="NBK22_001559"
     CDS             complement(13331..>13774)
                     /locus_tag="NBK22_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA helicase"
                     /protein_id="HCD2414748.1"
                     /translation="KMLYRILQDQINNQRYTLVFFNNIETMIKTFSVYKQKITKLTYV
                     HSEDVFRFEKVEQLRNGHFDVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGR
                     VGRKLECPTGKVLFFHEGVSMNMIQAKKEIQRMNKLALKRGWIDE"
BASE COUNT         4087 a         2687 c         1883 g         5117 t
ORIGIN      
        1 cactcatgca ttcctatttt taagtacaca atagctgtgg ctaatgacaa agaaccacta
       61 cataataaat cattagtgat tctttatcat ttctgtccca ctcccagttc gatttatgtt
      121 taactactct agattgaccc tatcttaaca taattctttt tacgcatctt ttctttctac
      181 tattcattac taaattaata aaaagaagcg aaggataaat ttataacatc cttcacttct
      241 tttaatctta tttatcttca gtattttgtt tatctcgttc aagtacttct tttaaatact
      301 ttcctgtata tgatgacttt gtctgagcaa tatcttcggg tgtgccagtc gcaacaatag
      361 taccaccgcc actaccacct tcaggaccta agtcgataat atagtctgct gttttgataa
      421 catctaggtt atgttcaata attacaacag tatcaccatt ttcaactaat cggtttaata
      481 cttttaataa tctactaata tcgtcaacat gtaaccctgt tgtcggttca tctaggatat
      541 aaatagattt accagttgaa cgtttatgaa gttcagatgc aagtttcaca cgttgagcct
      601 caccacctga taacgttgta gcttgttgac ctaatgtgac gtatccaaga ccaacatcaa
      661 ctagtgtttg taacttgcgc ttaatcttag gaatattttc aaaaaattgt gttgcttctt
      721 caacagtcat ttctaaaatg tcagcaatat ttttaccttt gtaagtaacc tctagtgtct
      781 cacgattata tcgtttacca tcacacactt cacaaggaac ataaacatca ggtaaaaaat
      841 gcatttcaat tttaataata ccgtcacctt tacaagcttc acagcgtcca ccttttacat
      901 taaaactaaa acgccctttt tgatatcctc gaattttagc ttcatttgtt tgcgcaaaca
      961 catcacgtat atcatcaaac acaccagtat atgttgctgg attagagcgt ggcgttctac
     1021 ctatcggtga ttgatcaata tcaataattt tatcaagttg atcaatacct tcaatcttat
     1081 cgtacaatcc tggctttact ttagatttat taattttttg agctaatgat ttgtataata
     1141 cttcatttac taatgagctt ttaccagaac ctgatacacc tgtaacaacc gtcatgattg
     1201 atagtggtat gtccacatca acccctttaa gattgttgct tctagctcca cgtatagaaa
     1261 ttttacgatc tgaagccggt ctgcgatatt caggtacttc aatacgtttc ttaccactca
     1321 agtattgtcc tgttaatgat tttttatctt tcattacctt ttgaggagta ccactagaca
     1381 caatctgccc tccatgttca ccagcaccag gacctatatc cactaagtaa tcagccgcac
     1441 gcattgtatc atcatcgtgt tcaactacaa ttaaagtatt tcctaaatct ctcatttctt
     1501 taagtgtatt aattaatcga tcattatctc tttgatgcag tccaattgat ggctcatcta
     1561 atacatataa gacaccagtc aaacgcgacc caatttgcgt tgctaatcga atacgttgtg
     1621 cttcaccacc tgaaagtgta cctgaagctc tatttagcgt taaatattca agtcccacat
     1681 tatttaaaaa agtgagtcgg gaaataattt ctttcaatat ttgatttgca atcgcttgat
     1741 cttgttctga caaatcaatg tttttataat agttcagcgc ttgactgatt gaatattcga
     1801 ctacttcacc aatatttaaa ccacctacat aaacagataa cgcttcacga ctcaatcgct
     1861 ttccatgaca agtttcgcaa ggtagttcag tcatatattt actcatcatt tcacgtgtat
     1921 attctgaagg agattcatgg aatcgtctac ttatattagg aactacaccc tcgaaaacca
     1981 ttgttcgttt tctagtacca ccttgacgtt gtgtaaatgt aaattcaatt tctttgtcac
     2041 cagaaccata caataaaata tcacgttgac gttctgttaa ctttttaaaa ggtttatcca
     2101 tattgatttt ataaacttca caaacacgtt ttaacaatgt tggataaaaa tcagaactcg
     2161 tcggtatcca aggttctatt gcaccttcgt ttagcgtctt atctttgtcg ggaacaacca
     2221 agtctacatc gactgttaac ttttggccta agccatcaca tgtcggacaa gcaccaaaag
     2281 gactgttaaa gctaaacatt cttggttcta actctccgat tgaaaatcca catataggac
     2341 aagcgtggct ttctgaaaac ttaaggtctt ccccgtcaat gacatcgact gttaattgtc
     2401 cttctgaaag ctctaaggca gtttctatag agtcagctag tcgtgtttca attccatctt
     2461 taacaactaa tcggtctaca acaacttcta ttgtatgatt cttgttctta tctaaagtag
     2521 gtacatcatt tacatcaaca atttcgccat cgattcttaa acgtacataa ccttttttac
     2581 caatatcttc gattagcttt tcatgactac ctttacgatg agcgatgaca ggtgctaata
     2641 attgaatctt tgtacgtgcc tctaattcca taatgcggtc aaccatttgt tgtactgttt
     2701 gcgattcaat ttctatattg tgatttggac agtaaggttt accaacacgt gcatataaca
     2761 aacgtatata atcatatatt tctgttactg ttgctacagt tgatcttgga tttttacttg
     2821 ttgttttttg atctattgaa attgctggcg ataatccttc aattgtatca acatctggtt
     2881 tgtccatttg gcctaaaaat tgacgcgcat aggcacttaa tgattcaaca taacgtcgtt
     2941 gtccttcagc atatatagta tcgaatgcta atgacgattt acctgaccca gataaacctg
     3001 tcataacaat taatttattt ttaggtagtt cgatatcaat atctttcaag ttatgcgcac
     3061 gagcaccttt tactactatg gatggttctt tcatttactt gtcacccttc tgcttttaat
     3121 tcaaataaca tatctcttaa ttctgtagct ttctcgaaat ctaaatcttt cgctgcttgt
     3181 ttcatttctt tttctatatt gtcgattgtc ttttgacgtt cttttttcgt catcttctta
     3241 ggtatcacag tttgtgcttt gtcattattt tcgtcatttt caacagtagc actaattaaa
     3301 tcatgtattt ttttattaat tgttttaggt gtaataccat gtttttcatt atgtttcatc
     3361 tgtatttctc gacgacgttg tgtctcatca attgcatact tcatcgaatc agtcatttta
     3421 tcggcataca taatgacttc acctttatcg ttacgcgcag ctctacctat tgtttgaatt
     3481 aatgagcggt tagaacgtaa aaacccttct ttatctgcat ctaatatgac aactagagaa
     3541 acttctggta tatcaatacc ctctcttaat aaattaatac ctacgataac atcatatgta
     3601 cccattcgta agtctctaat tatttcgatt cgttctaatg tcttgatttc tgaatgcaga
     3661 taattaactt taatacccgc ttctttcatg tatgtggtta aatcttcact catcttttta
     3721 gtgagcgttg taacaagtac gcgttcatta cgctcaaccc ttgtttgaat ttcacttaat
     3781 aaatcgtcaa tttgattttc agtaggtcta acctcaatct taggatccag taaaccagta
     3841 ggacgaataa tttgttcaac catcttatcc gtatgttcaa tttcgtatgg tccaggcgtt
     3901 gcagatacat acacaagttg ttttgtcttt tcttcaaatt cttcaaattt aagtggacgg
     3961 ttatctaatg cactcggtaa tctaaatcca tgatccacca aaacttgttt acgcgctctg
     4021 tctccgttat acatgcctcg aacttgtggt aatgtcacat gtgattcatc aatcattact
     4081 aaccaatcat cgccaaagta atccaataaa gtatacggtg tcgaacccag tggtcgcaaa
     4141 gttaaatgta cggaatagtt ttcaattcct gaacagaatc ccatctctcg catcatttct
     4201 aaatcatagt tggtacgctg ttctaacctt tgcgcttcta gtaatttatt ctcatctcgt
     4261 aattctttca atcgttcttc caattctttt tcaatacgtt caatcgcaac ttttaacttt
     4321 tcttcacgtg ttacgaagtg agaagctggg aatatcgcaa aatgttctct ttctttcaac
     4381 acttcacctg ttaggtagtt aacttctcgg atacggtcaa tctcatcgcc gaaaaactca
     4441 acccttatac aaagttcttc tttagaggct gggaatattt caactacatc accacgcact
     4501 cgaaacgttc ctcgttggaa atcgatgtca tttcgtgtat attgcacatc tacaagtttt
     4561 ctaagtaatt cacttctatc catttccata ccaactcgaa cacttactac taaatcttta
     4621 tattcttcag gattacctaa accatatata caacttacac tagcaataat aattacatca
     4681 tcgcgttcaa ataatgcact tgtagcagaa tgtcgtagtt gatcaatttc atcattgatt
     4741 gaggcatctt tttcaataaa agtgtcagta gacggtacgt atgcctctgg ttgataataa
     4801 tcatagtaac ttacaaagta ttccaccctg ttttcaggaa aaaattcttt aaactcacta
     4861 tataattgtc ctgctaatgt tttgttatgc gcgataatta acgttggttt cccaacttct
     4921 ttaataacat tactcatcgt aaatgttttc cctgtgccag tagcacctaa taaagtttga
     4981 tgtcttttcc ccgctttaat accttccacg atttctttaa ttgcttgcgg ttggtcacct
     5041 tgaggctcaa aatcagaatg tattttaaaa ggataatgtt caaccattgt cacatatgcc
     5101 tcctctttca acgtattatt tatatttccg ctcaactctt tgctttctca ttacatattt
     5161 tagcaaagta gtcacacaaa aagcaaacgt ttgttcgtaa aaatgtcgaa caaagaaaaa
     5221 caaacatagt tgtgctaact atgcttgcct taaatcaaat cgtttatctt gttattttta
     5281 gtatgtaaat acacattata ttaatcatca ttttgtttca acaaatttgt ttgaaacatt
     5341 attttaaagt taatcttagc gatcttcatc ttgatgttta tgaaattcga gttgatctat
     5401 aattaaataa ccagctaata atgacactac atcaataaaa ataatccact cgttatggaa
     5461 atactcttta tagattgagg caccaattaa aattaatgtc agaatagtac cgacccattt
     5521 acttcttgtt attacactaa ataatactac caagacacat ggaaagaatg ctgcgctaaa
     5581 ataccatatc attcattttc ctcttttctt ttatttaaaa tgttcatggt tgtttctctt
     5641 aattctgttc taggtataaa gttttcagtc aacatttctg gaatgatatt attaataaaa
     5701 tcttgtacag atgctaaatg gtcaaattga ataattgttt ctagactcat ttcataaatt
     5761 tcgaaaaata attcttcggg attacgtttt tgtatttctc caaatgtttc ataaagcaaa
     5821 tcaattttat cagcaactga aagtatttgg ccttctaatg aatcatcttt accttcttgc
     5881 agtcgttgct tataaacatc tctatattgt aatggaattt cttcttcaat aaaggtctct
     5941 accatttctt cttcaacttg cgaaaataat ttttttaatt cactactcgc atatttaaca
     6001 ggtgttttta tatcaccagt aaacacttcg gcgaaatcat gatttaatgc tttttcatat
     6061 aagcttttcc aattaatctt tcgtccatga tattcttcaa ctgttgctag atattgtgca
     6121 attttagtta ctttaaagga gtgtgctgca acattgtgtt caaaatattt aaattttcca
     6181 ggtaatctta taagtctttc catatctgat aatcttttaa aatattgatg tacacccatt
     6241 tcaattacct cctccattaa ttaatcataa attatacttt ctttttacat atcaatcaat
     6301 taaatatcat ttaaatatct tctttatata actctgatta aatgatacca aaaaatcctc
     6361 tcaacctgtt acttaaacag gctaagagga tagtcttgtc ttgatatatt acttagtgga
     6421 tgtaattata ttttcctgga tttaaaattg ttcttgaaga tttaacatta aatccagcat
     6481 agttcatttc agaaacagta attgttccat tagggtttac agattcaaca acaccaacat
     6541 gtccatatgg gccagcagct gtttggaaaa tagcgccaac ttctggtgtt ttatctactt
     6601 taaatcctgc aacttttgct gcgtaattcc agttatttgc attgccccat aaacttccta
     6661 tacttctacc taattgtgca cgacgatcga aagcataata tgtgcagttt ccataagcat
     6721 ataagtttcc tctgttagca actgatttat tgtagttatg tgcaacaggt acagttggta
     6781 ctgatttttg tacttgagca ggtttgtatg ctacattaac tgtcttagct actgcttgct
     6841 taggtgcttg cttaactact acttttttag acgcttgttg tacaggttgt tttactacct
     6901 ttttagcttg gcttgctttt cttactggtg ttttaaccgc tttagtttgt ttcactttat
     6961 tttgaggcac aagtgaaatc acgtcaccag gaaaaattaa aggtgttaca ccaggattgt
     7021 atgaatataa ttgattcaac gttaagtgat gctctaatgc aatcttatat aatgaatcgc
     7081 cagcaactac tgtataagtt gtcggtgatt gcgtttgtgc ttgaacattt gatacataat
     7141 tatgttgaac aggtgttttt acttgtgtgc catgttgttg tgcatgtgct gcattattta
     7201 aagctaaaaa agctaacact gacgaaaccg tcactgtaag agattttttc atcttgctgt
     7261 cattcctttg ctgttagtat tttaagtatg caaatactat agcacaatac attttgtcaa
     7321 aagctattgt tataacgatg taatcaaatg gttaacaatt taaaaagaat gcaacctttt
     7381 attatagtgt aaaatgtatt cataccatgt aattgagaac gttttcaata attaattcaa
     7441 taccttgaaa atcgtcatag gtaatattac taaatgcaca ctgcatatgt tgttttaaca
     7501 aacacaactt ttaaaaaata tattctaact ctatctaccg aattgtactt aaatattcat
     7561 aaacaaatca tattccaaaa tctaatttac aatttattta gctaccttta aaaaaaccaa
     7621 aaaccgatgc ccttttagag cctcggtttt aaaatatata ttaatcgtgc gacattgtct
     7681 gtcttaaata tgattcgata aatggtccaa tgtctccatc catcactgca tcaaccttac
     7741 ctgtttcttc gttcgtacga tgatctttca ccattgagta tggatggaaa acatatgatc
     7801 taatttggct tccccagccg atttcttttt gttcgccacg aatttcagcc atttcacgtg
     7861 cctgctcttc caattttaat tgatataatt tagactttaa cattttcata gctgcttcac
     7921 ggtttttaat ttgagaacgt tcattttggt tattaacaac tatacctgag gggtggtggg
     7981 taattcgtat tgccgattca gttttgttaa tatgctgacc acctgcacca gaagctctga
     8041 atgtatcaac tgtaatatca tccggattga tttcaatctc tatttcatca ttattaaaat
     8101 ctggaataac gtcgcatgat gcaaatgatg tatgacgacg tcctgatgaa tcaaatggag
     8161 aaattcgtac tagtcggtgt acaccttttt cagcttttaa ataaccataa gcattatgcc
     8221 ctttgatgag caatgttaca cttttaatcc ctgcttcatc cccaggtaga taatcaacag
     8281 tttcaacttt aaagcctttc ttctcacaat aacgttgata cattctaaat agcatattag
     8341 cccaatcttg agactccgtg ccacctgcac caggatgtaa ctctagaatt gcgttattgg
     8401 catcgtgagg cccatctaat aataattgca attcgtattc atccacttta gccttaaaat
     8461 aaatgacctc ttgctctaag tcttctttca tttcttcatc aaattcttct tgtaataaat
     8521 cccaagtagc atccatgtca tctacttctg cttgtagtgt tttataacca ttaactattg
     8581 cttttaacgc attattttta tctataatat cttgcgcttt cgtttggtta tcccaaaaat
     8641 taggttctgc catcatttct tcatattctt gaatattagt ttctttgttc tctaagtcaa
     8701 agagaccccc taatttgtgt taaatcttga ttatacttat ctatatttcg tttgatttct
     8761 gataattcca tagcattcgc tcctatttat attacaattc aagtcattga tttgtatctt
     8821 ttataatgct aaattttaac ataattttgt taaataacaa tgttaagaaa tataagcaca
     8881 ctgacaatta gtttatgctt ttattgttaa aaatcagtac atttatcatc gacatatgcc
     8941 taaacctatt ttttaaaact aagtacataa caacgtttaa caacttcttc acatttttta
     9001 aagtatttaa cgcttgtaaa ataaaaagac tcctcccata acacaaacta taggtgttta
     9061 attggaagga gttattttat atcatttatt ttccatggca atttttgaat tttttaccac
     9121 taccacatgg acaatcatcg ttacgaccaa cttgatcgcc tttaacgatt ggtttcggtt
     9181 tcactttttc tttaccatct tcagctgaaa cgtgcttcgc ttcaccaaac tctgttgttt
     9241 tttcacgttc aatattatct tcaacttgta ctacagattt taaaatgtat ttacaagtat
     9301 cttcttcgat attttgcatc atgatatcaa ataattcatg accttcattt tgatagtcac
     9361 gtaatggatt ttgctgtgca taagaacgta agtgaatacc ttgacgtaat tgatccattg
     9421 tgtcgatatg atcagtccaa tggctatcaa tagaacgaag taaaatcata cgctcaaact
     9481 cattcatttg ttcttctaag atatcttttt gactttgata tgctgcttca atcttagccc
     9541 aaacgacttc gaaaatatct tcagcatctt tacctttgat atcatcctct gtaatgtcac
     9601 cttcttgtaa gaaaatgtca ttaatgtagt cgatgaatgg ttgatattca ggctcatcat
     9661 ctgctgtatt aatatagtaa ttgatactac gttgtaacgt tgaacgtagc attgcatcta
     9721 caacttgaga gctgtcttct tcatcaataa tactatttct ttcgttatag ataatttcac
     9781 gttgtttacg taatacttca tcgtattcta agatacgttt acgcgcgtcg aagttattac
     9841 cttctacacg tttttgtgct gattctacag ctcttgatac catttttgat tcaattggtg
     9901 tagagtcatc taaacctagt cggctcatca ttttctgtaa acgttcagaa ccaaaacgaa
     9961 tcattaattc atcttgtaac gataaataga agcgactatc ccctttatca ccttgacgtc
    10021 cagaacgacc acgtaactgg tcatcaatac gacgagattc atgtcgctct gtacctatta
    10081 ctgctaaacc gcctaattcc tctacgcctt cacctaattt gatatctgta ccacgaccag
    10141 ccatgttagt ggcaatagta acggcacctt tttgtccagc gcctgcaaca atttcagctt
    10201 cacgttcatg atttttcgca tttaacacat catgacggat accacgtttt ttaagtaaat
    10261 ttgaaatata ttcagaagtc tcaactgcaa cagtacctaa tagcactggt tgccctgcct
    10321 tgtgtttttc aacaacatct tctactactg catcaaattt acctttttgg ctaatgtaaa
    10381 ttaaatcaga cttatcgtta cgttgcacag gtttatttgt cggaatttga gttactgtca
    10441 tgttataaat atttctaaat tcttcttctt cagttttagc tgtacctgtc atacccgcaa
    10501 gtttattgta cattctgaaa tagttttgga atgtaataga cgccatagtt ttagattcat
    10561 tttgaatttg aacgccttcc ttcgcttcaa tagcttggtg taaaccttcc gagaaacgac
    10621 ggcctggcat tgtacgtcct gtaaattgat cgacaattaa tacttcgcca tcaacaacca
    10681 tatagtctac gtcacgttgt aatgtaacgt gcgcacgtaa agctgtgttg atatgactaa
    10741 taacatcaac attttgtaca tcatataagt tttcaacttt gaacatacgt tcagctttat
    10801 ccgcaccttg ttctgttaaa tgtacagctt tcgttttttc atcgtattta taatcttcgt
    10861 cctgttttaa cattttcgca aaaacatttg cttgtgtata aagtgacgtt gacttttcag
    10921 cttcaccaga aataattaat ggcgtacgtg cctcgtcgat taaaattgag tcaacctcat
    10981 caatgattgc aaaatgtaat ggacgcatta ctctatcttc agaataattc accatgttat
    11041 ctcgtaagta atcaaaacct agctcattat tagtactgta agtaatgtct tgtgcgtatg
    11101 cttcacgttt ttcttctgtc gtcttactgt ttaagtttaa tccgacagtc aaacctaaga
    11161 agttatataa ctcagccatt tcttcacttt gaacacttga taagtattca ttgactgtaa
    11221 taacgtgaac acctctacca gctaatgcat ttaagtatgt tggcattgtc gctgttaatg
    11281 ttttaccttc acctgttctc atctcagcga tatcaccttt atgaattgca ataccaccca
    11341 taatttgaac tttatatggt gtcatattga atacacgttt agagccttct ctaacaagtg
    11401 catatgcttc tggtaaaatt ttatctaaat aatcattttg ctttttgaca ttatcaatgt
    11461 cagctaattc tgtttggaat tgtttcgttt tattacgaat ttcttcatca gttaaaattg
    11521 ccgttttttc ttctaaagcg attactttat cagcaagttt acctaactgt ttaatttctt
    11581 tattattgcc atcaagaatt tttgataaaa atcccatttc gttcgctcct ttagctaaaa
    11641 aactgtttgg cctacaacaa tatatcttat catttatagt tagaaaatta tacttattta
    11701 ctcatttgta gaatcaatat aaatatattt atgacatact tcattcacat tctgttgtca
    11761 acaagtttat cactaataaa tatattctca atacgcaatt atacttccta ataaattata
    11821 ttataaatat tttacgattt tcgactcgga ctatacaata gactgacata ctattattaa
    11881 cttaacattc aaatatatac atccattaac attagcatag tcactatgtt tcattcaaca
    11941 aattacatta tcgaactatg aaatagtcat aatttgcttt tggagtataa aaaagcactt
    12001 gtgcaaaaac acaagtgctt taaacttaat ttattgttca ctagtttgaa tcaagccata
    12061 tttaccgtct ttacggcggt aaacgatact tgttccatca gtttctctgt ctgtgaatac
    12121 aaagaagtca tgacctaata gattcatttg taataccgct tcttctgaat ccattggttt
    12181 taagctgaat tcttttgaac gaataatttc tatctcgtta tcatcgtaag cgtcattatc
    12241 aacttgtgtt tcttgcattt cttgtaattc ggcaacaaac acttcttgat ctcctcgatc
    12301 acggctctta cgattaatac gtgttttata ttttcgaact tgtctttcaa gtttattatt
    12361 aattaaatca atacctgcgt ataaatcatc gtttcgctct tcagctctta acgtaacatt
    12421 tttcaatgga attgttactt caattttagt agctgaattt gaataagttt taactttaac
    12481 atgcgccact gcatttggta cgtcattaaa ataacgttcc aacttaccaa ttttttcctc
    12541 aatatagttg cgaatagcat ctgtgatagt gaggttatct ccatgaattt caaatctaat
    12601 catagtaatc tctccttaaa cctctttatt ggtaactctt tattatattt taacattttt
    12661 acgctatcgt gcaaacgcaa acactttgaa ttttctgata tttttagcat ataatttaca
    12721 ccctgcacga tgaattgtta atccagttgt ataaatatcg tcaacgagta atatttcctt
    12781 gccatttaaa tctaattccg tatcaataat aaatggattt tcatctgcca gacgctcttt
    12841 cttagttaaa tgagactgtt ttggtctatt tgacatcttt aaaatcttat caaagcgaat
    12901 ccctttagct tttagtactg cttctaccgg gttaaatgtt ctagataaat catgtgccgg
    12961 cgaagaagga attggcacaa tatagtcata agatgtttgt ggtatttcaa tcaaatgtgc
    13021 caataattca cataaataat agtctttcaa aaatttatac tgatgtatca tctctttcat
    13081 taaaccgtca tattgaaatt gacaatataa ttgttccatt aaattaaagt gtgccgatag
    13141 aaacttgcag tctaaacaat acgcttcatc ttgatttaag tgttttaagc accttgaaca
    13201 tcgccttgct ttaatatcaa gtttaatatt gtcccaattc tctttgcatc tgtcacataa
    13261 tctattaggt ttcttgaaca aattataaat ggttatattt tcatataact tagcaccaca
    13321 actcaaacaa ttattcatca atccaacctc tttttaatgc taatttgttc atcctttgaa
    13381 tctctttttt agcttgaatc atattcatac ttactccttc atgaaaaaac aatacttttc
    13441 cagtaggaca ttctaatttt cgtccaacac gtccagcaat ttgtattaaa gcctcttgag
    13501 tatattgatg tgcatcgata acaacaacat ccaaatttgc cattgtaaat ccacgttcta
    13561 atattgtcgt agtaaaaatg acatcgaaat gtccattcct taattgttca actttttcaa
    13621 agcgaaaaac atcctcgcta tggacgtatg ttaatttagt aattttctgc ttataaaccg
    13681 aaaatgtttt aatcattgtt tctatattgt taaaaaacac cagtgtataa cgttgattat
    13741 taatttgatc ttgtaaaatt cggtataaca tttt
//
LOCUS       DAHHYJ010000072        13445 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000072 DAHHYJ010000000
VERSION     DAHHYJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.072"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(429..1712)
                     /locus_tag="NBK22_001560"
     CDS             complement(429..1712)
                     /locus_tag="NBK22_001560"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCD2414749.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLAQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTNVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(1990..3390)
                     /gene="dnaB"
                     /locus_tag="NBK22_001561"
     CDS             complement(1990..3390)
                     /gene="dnaB"
                     /locus_tag="NBK22_001561"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCD2414750.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSTEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(3422..3868)
                     /gene="rplI"
                     /locus_tag="NBK22_001562"
     CDS             complement(3422..3868)
                     /gene="rplI"
                     /locus_tag="NBK22_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCD2414751.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVEVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(3865..5832)
                     /gene="gdpP"
                     /locus_tag="NBK22_001563"
     CDS             complement(3865..5832)
                     /gene="gdpP"
                     /locus_tag="NBK22_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCD2414752.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIILFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIATAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(5847..6785)
                     /locus_tag="NBK22_001564"
     CDS             complement(5847..6785)
                     /locus_tag="NBK22_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCD2414753.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAVTILL
                     LVIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(7079..8047)
                     /locus_tag="NBK22_001565"
     CDS             complement(7079..8047)
                     /locus_tag="NBK22_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD2414754.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHIFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(8107..8205)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8410..8739)
                     /locus_tag="NBK22_001566"
     CDS             complement(8410..8739)
                     /locus_tag="NBK22_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCD2414755.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     ATLRFFF"
     gene            complement(8736..9431)
                     /locus_tag="NBK22_001567"
     CDS             complement(8736..9431)
                     /locus_tag="NBK22_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCD2414756.1"
                     /translation="MATHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIIL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
     gene            complement(10082..11368)
                     /gene="serS"
                     /locus_tag="NBK22_001568"
     CDS             complement(10082..11368)
                     /gene="serS"
                     /locus_tag="NBK22_001568"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCD2414757.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQYGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(11458..11674)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11747..13261)
                     /gene="hutH"
                     /locus_tag="NBK22_001569"
     CDS             complement(11747..13261)
                     /gene="hutH"
                     /locus_tag="NBK22_001569"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCD2414758.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSALTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYEEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
BASE COUNT         4119 a         2646 c         1995 g         4685 t
ORIGIN      
        1 ttcttaagca caagatagat tatagcacag ccaatcttgc catgacaaga tattttttac
       61 ataattttgc tattattttt tgaagaatgt tataagtgaa aaattgcatt ttattggcag
      121 tctatttatt ataataatta cgctattttt gaaatttttc tattttaggt gctttcattt
      181 acacttacgt ctactctatt ttaatagcta ttttcgacat cagacttctt acattcaagt
      241 atttcatctt aattttcgtt aaatatcatc taatcttcta aattatgact agcaaatatc
      301 gcctttacgt tagtattttt tatctaaata atcgaatcga tacagaagca agcataacaa
      361 aagcctccta ccaataaaga tagaaggctc atgtatttat aatcattgtg tatgacttat
      421 ataaagttct accacaattc ttttaatagg tttgtttgtt ctctatctgg accaactgag
      481 aagatagaaa tttgtacatt acataattct gaaatacgct ctaaatattt acgtgcattt
      541 tcaggtaatt cttctaaagt acgcacattt gttacgtctt ctgtccaacc tggtaactct
      601 tcaaagattg gtttacaacg ttttaattga tctaagtttg ctgggtactc agtaatttct
      661 ttaccgtcta attcataagc tgtacagatt ttcactgtgt ctaggcctgt taaaacatcg
      721 attgagttaa tagataaatc tgtaatacca cttacacgac gagagtgacg taatacaact
      781 gaatcaaacc aacctacacg acgtggacgt cctgttgttg taccgtattc acgaccaacc
      841 tctctaatat gatgtccatc ttcatcgaat aattcagtag ggaatggacc atcaccaaca
      901 cgtgatgtat aagctttaca tacaccaatt acctttgaaa cgaatgtagg acctacacct
      961 gtaccaacag taacgttacc tgcaattgga ttacttgatg taacgaatgg atatgtacca
     1021 tggtcgatat ctaacattac accttgcgca ccttcgaaaa gtaccttttc atctgctaca
     1081 aatgcatcgt ctaagatttt tgatgtgtct gttacaaatt cttttagacg ttgacctgct
     1141 gcataatatt cttcaaagat atcatcaaat gatggacatg tttcgttaaa catacctttg
     1201 aaatatgctt gtttatattc aatgtttgat tttaataatc tttcgaatgt ttctttttca
     1261 agtaaatctg ccatacgaat accgatacgt tgaactttgt ctacatatgc tggaccgata
     1321 ccttttttag ttgtaccaat cttattgtca ccacgtaaac gttcttcata ttcatcttgt
     1381 gctaagtgat atggtaaaat cacttgcgca cgattagata tacgtaaatt acttgtagga
     1441 atgccacgtt catttaatcc gtctaattct ttcaatagtg caactggatc aacaacgact
     1501 ccgttaccga ttaccgctaa tttgtctttg taaaagatac cagatggtac taaatgtaat
     1561 ttatatgttt ctccgccaaa ttgaatggta tggcctgcat tattaccacc tgaaaaacgc
     1621 gcgataacat ctgactgttc tgccaagaaa tccgttattt ttccttttcc ttcgtctccc
     1681 cattgtgtcc caactactac gattgatgac atgtgagcac ctccaagttt ttctcattta
     1741 accatttgat attctaccaa tatcgagacc ataatgcaaa taaaaatcga acattgattt
     1801 tcggcctttt taaaagaaaa atcattttaa caaatcgttt tcaacgtcct aaaaaccctt
     1861 taacagaggc gttgacagta ctaatttatc actttcaata cacaaactta acatatatca
     1921 acccgttcag aacaaaataa aaaccgtaca attttatcat cttaatgatt attgtacgga
     1981 aaaacttttt tacatcatat ctgcatgtgc ataatcgata tcggtaaatt tattatattg
     2041 tttcataaaa tgtaacttaa ctgtgcctgt tggaccgtta cgttgcttag caatgataat
     2101 ttcaatttca ccgttttcat cattcgtttg tggctcgaaa ccaccatcat cgtcatcatc
     2161 ttcatcgccg ccacggttat agtaatcatc acggtataag aatgcaacga tatcggcatc
     2221 ttgctcaatc gaaccagatt cacgaatatc actcatcatt ggacgtttat cttgtcgttg
     2281 ttcaacacca cgagataact gacttaatgc gataactgga cattctaatt cacgggctaa
     2341 tgcttttaat gtacgagaga tttcagaaac ttcctgttgt ctgttatcgg acgcacgtga
     2401 accactacct tgaatcaact gtaagtagtc aatcacaatc atgtctaagc catgttcttg
     2461 ctttaatcga cgacatttag aacgtaaatc attaattcga atacccggtg tatcatcaat
     2521 aaaaatcttc gtacgtgata atttacctac cgctatagta aaacgactcc aatcttcctc
     2581 agtcatagta cccgttctta agcggtttga gtcaacatta ccagaactac aaatcatacg
     2641 tgtggctaac tgatcagcac ccatctctag tgagaaaata ccaactgtat acatatcttc
     2701 atgcgttgca actttttgtg caatattaag tgcgaacgca gtcttaccta cagatggacg
     2761 cgctgcaagg ataattaaat catttcggtt gaaccctgct gtcatttgat ctaaatctcg
     2821 ataccctgta ggtatacctg gtgtttgacc actattttga tcaagctctt cagctgtttc
     2881 atacacttgt cctaagacgt ctcgaatgtc tttaaagcca tcgctttcac gagaagatga
     2941 tagctctaaa attcgacgtt ctgcatcact taaaatcgca tctagttcaa gttcatcatt
     3001 atatccatca ttggcaatac tatctgcagt ttgaatcaat ctacgtttta atgcatgctt
     3061 agaaacgata tccgtataat actgaacatt tcgtgtcgtt ggtacatttg tagataactc
     3121 tgcaagatat tgcgggccac ccgcttcatt caacgtacct tccgtcgata attgatccat
     3181 caatgttaca acatcaattt ctttattatc ttcatttaag tgcatcattg cacggaaaat
     3241 atgttgatgg gcacccctat aaaacgactc aggaagcaaa acttcctgag tagtattaat
     3301 caattctgga tctataataa ttgaacctaa gacagactgt tcagcttcat tgttatgtgg
     3361 catttgattt tgctcataca ttctatccat gaatggttac acctcttatt tcaatccaac
     3421 tttattgttc aactgtgtgt acgcgaattg taccttcaac ttctttatct aatttaacag
     3481 gtacattcgt atatcctagg gaatgaattc catttggtaa atccatttta cgtttatcaa
     3541 ttttaatatc atgttgtgct tttagtgctt cggcaatttg ttttgtactt actgatccaa
     3601 acaatttacc accttcacca gtttttgctg atacttcaac ttcaatgttt gataacgttt
     3661 cttttaatgc tttagcatct tcaatttctt gttggcgttc ttgttttgca cgttttttct
     3721 gtaactctaa ttgtttaagg ttacctggtg ttgcttctac agcataattc tttttcaata
     3781 agaagttatt tgcataacct actggtactt ctttaacttc acctttttta cctttacctt
     3841 taacatcttg tgtaaaaatt actttcatgc atcttcactc ctacttaatt gttctgtaat
     3901 tgcttgttgt aattgtgcta tcgcctcttc gactgtcaca cctttaagtt gtgttgccgc
     3961 attggttaaa tgtccaccgc caccaagtgc ttccattgtt aactggacat ttactgaacc
     4021 gagtgaacgc gcagatatac caatcagatt atcttcacgt ctcgcaacaa catatgatgc
     4081 ttcaatacct tctaaactta acagttcatc tgctgcttgt gcaactgtta ctggatgata
     4141 aattttatcg tctgaaccat gcgcagtggc tatgccatta tcttcaactt ttacagttcg
     4201 aattaattca gatcgattaa tgtaagtatc cacatcatct tttaagaaat gttgcgttaa
     4261 aatcgtatct gcaccatgtg cacgtaaata actcgctgca tcgaatgttc ttgatcctgt
     4321 tcgtaatgta aagtttcttg tatctacaat aatacctgca tacatcactg ttgattcaag
     4381 acgtgttaaa cgttgttctg ttggttgata ttccagtaac tctgttacca attcagctgt
     4441 cgaacttgcg tatggttcca tatatatcaa caatggatta gagatgaagc tttcaccacg
     4501 tctatgatga tcgataacaa ctttacggtt tgctttattt aagacatttt catctaaaac
     4561 cagttccggt ttatgcgtat caacaatcac tacggttgtc ttagatgtca tcatatccca
     4621 agcatcatct gatgttataa atcgctctct taactctggc tttttatcaa tttcgttcat
     4681 cacgcgtcgt aatgttggat caatgtcagt ctcatttaat acgatgtatg cttctaaatt
     4741 attcatcatt gcaaatctag acacaccgat tgctgcacca attgcatcta agtcaggacg
     4801 tttatgtccc atgataatga ctttgtcacc ctctgcaagg atatctttta acgcatgtga
     4861 gatcacacgt gctcttacac gagtacgttt ctccatcggg tcagtcttac cgccatagaa
     4921 acgcacatta ccattaatac ttttaattgc aacttggtcg ccaccgcgtc ctaatgctaa
     4981 gtctaggcct gattgtgata attcacctaa gtcgattaaa ttttcagtac cttcaccaac
     5041 accgatactc aatgttaatt gggcacgata accaacactt ttttcacgta attgactcaa
     5101 gatatcaaat ttagattctt ctaagtcagc taatattttt tgatttaaat aggctacgaa
     5161 ttgatcggaa ctgtatcttt tgaaaaatat attatactca gttgcccatc gactaatgac
     5221 acgcgttacc attgagttga tttccgaacg ctgcgtatca ttcatatttt gcgtaatctc
     5281 atcgtagtta tctaaaaata atgtcgcaat gattggttta gaattttcat atagttcatt
     5341 tgtttgtact tgttcagtta tatcaaagaa atagaggcag tgatcattct cagaataacg
     5401 tacttggaaa tgatactgat tatattctat ttcaacggat ttcactctat ctaattgctt
     5461 taaaatgttt ggaaatactt catttacaga ttcagaaatg acatttgctt ccatatgatc
     5521 tgtcataaat tggttaaccc attcgatgtg atcattttca tctaaaacaa tgataccaat
     5581 tggtaaatgt ttgattgctt tattatttgt tgttgaaatt tgagcactca aaccatctac
     5641 ataactatcc attttcatta aagcttgtct gaataaaatg atgctaacaa taatcatcac
     5701 gacaagaacg atagatgcaa ttagtgctat aagactatta aagataaacc atacacccat
     5761 taaaacaatt gctgtgatga tcatgatgac aaatggtatt agtaaagctt tcttagtgga
     5821 ctgccgattc attattccac ctctattcac tttttagaat tatttttcat gattcgcttc
     5881 aaattcaaac ttaaatcgat aacaccaagt agtcctacaa tatgtgtcgt aggtgtcagt
     5941 attgtaccga taaccaatag taaaatcgtt actgcattcg gcaaaccttt cgctttacca
     6001 aagaaatgaa taacacttaa accttgaata tacattacta atgataacac aagttggaag
     6061 tttaaaagaa tgctctggaa cacactcggt tgacctgtaa ataataaaca tatgataaca
     6121 ataatgtata tccataataa aataccgctc atttgccacg cgaaaagtgg cttaaataca
     6181 ggtgtagcga ttttaaattt tcgtaaaatc ggaaatgtaa cgattaagtt aattaagacg
     6241 attaaaaatg taatgataat gatgaaacct ggtaattgaa cggtcgcttg tctaaaccct
     6301 tcttctaata tttgggtcat attcgcatcg gcaccgctca tcgtaatcgc ttcatgtaat
     6361 gtttgcttga aaggttttac tatgctcgct gatggtggaa tccttccgaa tgtttgtagt
     6421 aacataaaag cgattaatga aattaagctc atcgctactg ttgttacgta taatattctt
     6481 tctttagacg ttctttcttt gagcaattga ccaataatta aacttgcaat taagactaat
     6541 atgatggcac ttaaaacgaa ggtattacct aaaacagttg ttataattac tgtaataagt
     6601 gcactaatcc cgaaagattg tattgattta ttccataaaa cgatacctgg tatggttgca
     6661 aataatgcaa atattagtcc taaaccaggc actagatata aagctaaagc gacaaatacc
     6721 aacgcaatcg ttgcaattat tgttgcttta ggttgtattt ttgaaaacac ataagccact
     6781 cccatatttt taactatagc tattatttta acctctttaa tggaaattaa caatttatag
     6841 attgtatgct tctatttcat ttaattgaat aataactttc atgttttata agtaattaac
     6901 atactcattt gaatcgcttt tgtgtgcttt cattttcaat atgattattt aatcccacta
     6961 catagcaatc aagcttgatt tagattcaca attcatttcc actctcaatg tactctagat
     7021 gtttttgaat atgataactg tgatttagtg gcttcattct ttgaaaatat atatattatt
     7081 acttacgctt aaaatgcttt aaatttaaga aatgatataa gttaggtgcc caggtactaa
     7141 agtttagtag gaatccatca tgcccaacat tatcaggcac gaagaaatga cgatgatatt
     7201 taaaacgttc acctaatgca cgaacttgat catccggata tagcaaatca tctatgaacc
     7261 ccatcgttaa cacttttgtt tctaaatttt taaaaatatg cgttacgtct gtgcgacctc
     7321 ggtcaatgtt gtgactatcc aatacatcta gcagtgtcag ataacaattc aaatcaaaat
     7381 gttctttaaa tttattacct tgatgttgtt ggtatgcgac tacttcatcc ggcgtgaaac
     7441 gttcatcata actttttgat gatcgatatg tcaaaaaacc taattggcgt gcaatactta
     7501 gaccttcctt accaccaaga tgaatggctt gccttgcaat ttcattgaaa gctctactat
     7561 aagatgatgt tcgacttgtt gcagcaagga taatggcttt atctacttca aactgttgat
     7621 tgtaaagtag ttccattgct tgcatacctc caagacttcc ccctattaaa atattaatct
     7681 tatcataacc aagggcttgt atacctcgtt cattcgctct gactatatct cttaatgtta
     7741 attttttagg aaaatgaggg tcgtttaaag gtgaacttga accgaaagga ctaccaataa
     7801 catcaaatgt taaaaattga taatcgtgaa tgggtatata tcccccatca ataatttctc
     7861 gccaccaacc cggataatca tctgttccat atgttaaatg attgccagtt aatgcatgac
     7921 aaactacaac taatggttgt ccatgataac cgacatgctc atatctcaaa cgcaagttat
     7981 ctatgacttc cccagattct gtaataaatt tccctaaatt taaagtatct actgtgtaat
     8041 ttgtcattgt tctttcctcc ttaaacaaaa aaacttctca ccctattgaa aagtaagaag
     8101 tctttatact tatcattcga gtaactcgtt ggttttagca ccgtgctata aagtcggttg
     8161 ctgaagtatc acagggccaa gtccctcaac ttctcacaat aagaaatatg aaattgttat
     8221 gtgttagttg agattcagtg atgaattact tttatcattt aaaatgttgt tatcattgtc
     8281 atgcgttagc caaatcgctt acgtatacac gattcccaat cttaacatag acgatttgta
     8341 tatcagaatt ttctgattac taacagttta cctaagttta aatatctgtt caatgatttt
     8401 cagttatttt taaaagaaaa atcgtaatgt tgccatgata acaatcccac taataattgt
     8461 aatagttaaa ctacgcgtga ttatagataa aataaccgtc ggaatgagcg cgataatgta
     8521 agggatgttt aatgtatacc cctcaccatg aggcgtctgt tgaataatgc tgtcaatgac
     8581 aagtgccgta aatagtgtga ttgggataaa tgatagccat cgaaccacaa catcaggcaa
     8641 ttgcactttt gaaatcatga taaaaggtat aattcgaatt aatagcgtta cgattccaca
     8701 caataaaata agtattaaca tgttcatatg agttatcatc gttccatcat cactcctaac
     8761 gctgctgaaa ttgtggctgc aattaatatt gctagatatg aaggcataaa catacttagc
     8821 gataacatca ttactatgac ggcaataatg agtactatgt aaattcttaa tcgcgattta
     8881 gtaattgatt caaattgcgc aatggccaaa aagataaaca tagctgtgat agcaaagtct
     8941 aaccctagcg tttgcggatt tgagatatat tcgccaaata aagccccagc tacacatgaa
     9001 attgcccaaa ataaatatgc tgtgatatta agaccatgca tccaacgatc attgatagct
     9061 tctcctttta aataaggtgt aatggcgacg ccaaacgttt cgtcagttac taatgaacct
     9121 aatccaacac ggttccaaaa cccatatgtc ttgaagtttg gtgcaagcga catacttaaa
     9181 aggaacattc ttgaatttac gataaataca gttagtacaa tcgctgatat cggtgtacct
     9241 gctataaaca acgcgcacat aataaattgc gcagcaccgg catatataac aagacataac
     9301 aagataattt ctaaaatact aaagttttga gacgaagcca caataccaaa tgaaatacca
     9361 acaccggcat aacccaataa tgttgggata cactcttgta cgccttgtct aaaacttaaa
     9421 tgtgttgcca tctcaattac ctcctttgcc actttatatt tatataatta cgaaaaaata
     9481 atatacctag tctaatacag ttctataccc taagcaataa cattagacac cagtttgtct
     9541 gaggttagac atactggtgt cttcaatcat cttcatatca actttttatt tttgagaatt
     9601 ttcaattttt tatttaagac tacctccata ttttctacgg atttgtagtt gtttttaagt
     9661 actaatttta taatttttta tatctaatga tgagtctggg atattgattc atgtaacact
     9721 cccttataat catcatcctc tccccctacc ctactccatc gatattactc atactacatc
     9781 aacgaaatca gttttttatc acttaatttc ctataatagt gatgctcaaa attgttacgt
     9841 tttagattgt tttagttcat cataattatc ccgtattgtt gctataatga aatgcgttca
     9901 ccccattaaa ccacaaactt aatttattgt tgttatgtgc attggctcac tattatactt
     9961 ttacagcaca aaaaaagtgg cgacagcttc gtcaccactt tttaaaatat tatttaaagt
    10021 atcttgccct tgctttaagt atacgtagat atatactttt taaagcttgt agctaaagcc
    10081 tttatttaac tggttttgaa atttgtgttt taccacccat aaatggtact aatgcttctg
    10141 gaattgttac tgttccatct tcattttggt aattttcaac aatagcagca aatgtacgtc
    10201 caactgctaa accactacca tttaatgtat gtgctaattc tggtttagct gctttgtcac
    10261 gcttgaagcg gatgttagca cgacgcgctt ggaaatccgt acagtttgag catgaactaa
    10321 tttctttata atcattgtag cttggtaacc aaacttctaa atcatatgtt ttgcttgcac
    10381 taaatccaat atcacctgta cataaaataa cacgacggta tggtaaacct aactcttcta
    10441 gaattgcttc tgcgtttgtt gtcatttctt ctaaagcatt ccatgaatct tcaggttgtt
    10501 caaaacgtac catttccact ttatcgaatt gatgtaaacg aattaatcct cttgtatctc
    10561 tacctgctga tcctgcttca ctacggaaac atgcagattg accagtgaat ttttcaggaa
    10621 gtacacctgg ttgaataatt tcattacggt agaaattcgt taatggtact tcagcagttg
    10681 gaattgtata taatccttct ttttctactt taaataaatc ttcttcaaat ttaggtaatt
    10741 gacctgtacc atacattgta tctgcgttca caagctgtgg taccatcatt tctgtataac
    10801 catattgtgt tgtatgtttt gtaatcatat agttcattaa agcacgctct aattgcgcac
    10861 cttcatttgt taaatataca aaacgcgcac ctgaaacttt tgctgcacga tcaaaatcag
    10921 ccattttcaa ttcttctaca atatcccaat gtgctttggg ttcaaatgaa aactcacgtg
    10981 gtgtacccca ctttttaact tcaacgttat cttcatcaga ttcaccttga ggtacatcat
    11041 cacttattaa atttggaata cgacaaagga tacctgtcat tttattatca atttcattta
    11101 attgactatc tttttcttta atatcgtcac ctaatgtgcg catttcagca atcacatcat
    11161 cagcattttc tttattacgt ttttttaatg cgatttcttc gcttacttta ttacgacgtg
    11221 ctttcatttc ttctgttgca ctaattaatt tacgtcgttg ctcatccaat tctaaaattt
    11281 catctacaac ttttggatca tctccacgta attcaatttt gctcttaact gtgtcaggct
    11341 catttctgaa taatctaatg tctaacatta accttcatcc tttcccaaat aattatcatt
    11401 tattatggaa tgacgtacgt ctttattttt tagaaaataa aaaaagacca catccctaca
    11461 agggacgtgg tctacgcgtt gccaccctat ttaacaattt aagttataaa gatacactaa
    11521 acctaaattg cacttcacta aaataacggt tatcaccgat tgttctttta aattaagtag
    11581 gtagattcat atatatgttg attcttgttc acactaacca caagctctct gatatcgaac
    11641 actatatatt acttgtccta cgaacaatgt cttattaagt tatttttaat atagcaaact
    11701 atatttgctt tttcaagtaa cgatttcaaa catcactcat gtcgatttag tgacatgcag
    11761 tcgtttgata aattgattgc tttaaatact gtgcaaccgc ttcaatatct ttatgaaatt
    11821 gacggtcatg tgtaatggat ggcacgatac ttcgaaactc ctcatattta cgacgtgttt
    11881 ttggtgataa accttcaaca ccttttaact ctgctgcttg taatgcaata acacattcga
    11941 ttgccagcac acgtcttgca ttttcaataa tttgataacc atgtctagca gctgtagttc
    12001 ccatagatac gtgatcttct tggttcgcag atgaagtgat agaatcaaca ctcgctggat
    12061 gcgctaaagt tttattttca gaaacgagac ttgcagcagc atattgcata atcatcgcgc
    12121 cactttgcaa tcctggctct ggactaagaa atgctggtaa atcaccattt aattgaggat
    12181 ttactagtcg ctctagacga cgttccgata cgtttgctaa ttcacttaca cctaatttaa
    12241 gatgatctaa tgcaaaagca ataggttgtc catggaagtt accacctgaa ataacaaacg
    12301 tttcatttgc ttcctcaaat ataagtggat tatcattagc cgcattcatt tcaaattcta
    12361 attgctgttt aacataattg aatacttgaa aactcgcgcc atggatttgt ggtatacaac
    12421 gcaacgtata tgcatcttgt acacgtattt ctgcttgtcg cgttgttaat gctgatcctt
    12481 ctaaccaatc acgcatacgc gctgccacat taatctgttc ttgaaaatta cgaacagcgt
    12541 gcacatcatg tcgatatgca tctataatgc cattaagaga ctgatgcgtt aatgcagcaa
    12601 tccattcaga ttggtaacct aaatcttctg cttctatata actaatgaca ccttgagctg
    12661 tcatagcttg cgtaccatta atcaatgcta aaccttcttt agcctgaagg ttcaaaggtt
    12721 gtctatttaa ttctcttaat acatcgtcac tatccttttc ttcccctctg tacaatactt
    12781 tcccttcacc aattaatgct aatgctaagt gtgataatgg cgctaaatct cctgatgcac
    12841 cgagagagcc ttgctgtggg attatcggta taatacgttc atttataaaa aattgtaatt
    12901 gtctcactaa ttctaaagtg gcacctgaat gaccttttaa taatgtattc aatcgtaaaa
    12961 tcatcatgac taatgctact tcttttgaaa atggctcacc tagtccacag gcatgtgagc
    13021 gtatcagatt cacttgtaat tcattatatt gcgtcgggtc tatacgtaca tcactaaata
    13081 acccaaaacc tgtagtaata ccgtaaaccg tttcctcatt ttcaataata cgttcaacta
    13141 ccgctctact ttttttgaca cgttctaacg catcatcaat aatttcaatc tttgattgtt
    13201 gttgtaaaaa tgatttaata tcctcaattg ttagtgtctc accatctaaa tataaagtca
    13261 tatatgttac ccccttgttt atattaagta acccatcctt cttgaagtat acgttttcat
    13321 ttttattgaa acaatggttt tacgtacatt tataacctat tatcagagca ctattgtagt
    13381 gcgttaaagg atattaagat tgttgtaagc atatttaata atttatctat tgacgaattg
    13441 catat
//
LOCUS       DAHHYJ010000073        13419 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000073 DAHHYJ010000000
VERSION     DAHHYJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13419
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.073"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            191..988
                     /gene="fdhD"
                     /locus_tag="NBK22_001570"
     CDS             191..988
                     /gene="fdhD"
                     /locus_tag="NBK22_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCD2414759.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNTTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPIQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITAVGFIRNGGFNIYSHPERIIDSEQ"
     gene            complement(1160..1930)
                     /locus_tag="NBK22_001571"
     CDS             complement(1160..1930)
                     /locus_tag="NBK22_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2414760.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKT
                     PTQERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNSDVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEEMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTIQSLIGEYAISKNH
                     KPIILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(2007..2561)
                     /locus_tag="NBK22_001572"
     CDS             complement(2007..2561)
                     /locus_tag="NBK22_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCD2414761.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLK"
     gene            complement(2682..3623)
                     /locus_tag="NBK22_001573"
     CDS             complement(2682..3623)
                     /locus_tag="NBK22_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD2414762.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVAPSYERGKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            complement(4022..4930)
                     /locus_tag="NBK22_001574"
     CDS             complement(4022..4930)
                     /locus_tag="NBK22_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2414763.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGEIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            complement(5144..6298)
                     /locus_tag="NBK22_001575"
     CDS             complement(5144..6298)
                     /locus_tag="NBK22_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCD2414764.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIRDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKAYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDEYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYKDDSQIRNPTSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            complement(6515..7420)
                     /gene="yut"
                     /locus_tag="NBK22_001576"
     CDS             complement(6515..7420)
                     /gene="yut"
                     /locus_tag="NBK22_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCD2414765.1"
                     /translation="MKIIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYAFARYINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGYTFKTAINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            7680..7982
                     /locus_tag="NBK22_001577"
     CDS             7680..7982
                     /locus_tag="NBK22_001577"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCD2414766.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
     gene            7996..8406
                     /locus_tag="NBK22_001578"
     CDS             7996..8406
                     /locus_tag="NBK22_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCD2414767.1"
                     /translation="MIPGEIITKSTEVEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            8403..10118
                     /gene="ureC"
                     /locus_tag="NBK22_001579"
     CDS             8403..10118
                     /gene="ureC"
                     /locus_tag="NBK22_001579"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HCD2414768.1"
                     /translation="MSFKMTQNQYTSLYGPTVGDSIRLGDTNLFAQIEKDYAVYGEEA
                     TFGGGKSIRDGMAQNPRVTRDDVNVADLVISNAVIIDYDKVVKADIGIKNGYIFAIGN
                     AGNPDIMDNVDIIIGSTTDIIAAEGKIVTAGGIDTHVHFINPEQAEVALESGITTHIG
                     GGTGASEGSKATTVTPGPWHIHRMLEAAEGLPINVGFTGKGQATNPTALIEQINAGAI
                     GLKVHEDWGATPSALSHALDVADEFDVQIALHADTLNEAGFMEDTMAAVKDRVLHMYH
                     TEGAGGGHAPDLIKSAAFSNILPSSTNPTLPYTHNTVDEHLDMVMITHHLNAAIPEDI
                     AFADSRIRKETIAAEDVLQDMGVFSMISSDSQAMGRVGEVITRTWQVAHRMKEQRGPL
                     DGDFEHNDNNRIKRYIAKYTINPAITHGISEYVGSIEPGKLADIVLWDPIFFGVKPEL
                     VVKGGLINSAVNGDANGSIPTSEPMKYRKMYGQYGGNLTSTSMTFVSKTAYENGINRA
                     LNLKRMVRPVKNIRQLSKADMKNNSATPKLDVDPQTYEVYVDGEKITSNAATELPLTQ
                     RYFLF"
     gene            10131..10583
                     /gene="ureE"
                     /locus_tag="NBK22_001580"
     CDS             10131..10583
                     /gene="ureE"
                     /locus_tag="NBK22_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCD2414769.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            10576..11265
                     /locus_tag="NBK22_001581"
     CDS             10576..11265
                     /locus_tag="NBK22_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCD2414770.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHHLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEGTRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            11278..11892
                     /gene="ureG"
                     /locus_tag="NBK22_001582"
     CDS             11278..11892
                     /gene="ureG"
                     /locus_tag="NBK22_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCD2414771.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            11892..12728
                     /locus_tag="NBK22_001583"
     CDS             11892..12728
                     /locus_tag="NBK22_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCD2414772.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(12917..13264)
                     /gene="sarR"
                     /locus_tag="NBK22_001584"
     CDS             complement(12917..13264)
                     /gene="sarR"
                     /locus_tag="NBK22_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCD2414773.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
BASE COUNT         4485 a         2347 c         2053 g         4534 t
ORIGIN      
        1 ttgtcaaatt tgcataagat tgatatttat taattgatcc cctagtatgt atactctcga
       61 attcaattta aatcaaacag attgctcgct attaattttt ataaatcaaa tttcaaactt
      121 aagcaaatat gttaaaattt taagcaaatt gctttaaaat aaagtttaag ctaattaaag
      181 gggttagata atgaacaaag atgtatcttt aggtcaacca attgttagat atgaagatgg
      241 aaaattattc aatacaacag atcaatatgt gacagaattt ccacttacaa ttatggtgaa
      301 tggtgaagaa tttgcgactg ttatatgcag tccaacaaac cttgaggagt tagtcattgg
      361 atttctggct tcggaaggtg ccattttaaa gcgagatgaa ttaaaatctg tgctgattga
      421 tgatagtaaa ggttttgccc atgttgaact taataaagat ttaggcgatc gctttcaata
      481 ttccacaaaa cgaatgattg catcttgttg tggaaagagt cgtgagtttt attttcaaaa
      541 tgatgctgct atcgctaaaa catctatgtc taaaatcact ttaactccaa tacaaattat
      601 taacatgatg actcgattac aaagtgcaag tcatatttac caagaaacag gtggcttaca
      661 taatgcagct ataagcgatg gtttgacatt ttttgtacat cgacaagaca ttggtagaca
      721 taacgctcta gataaattat atggattttg tattcagcga catattactg tccgagataa
      781 agtattaatt tttagtggac gtatttcatc agaaatttta attaaagccg cgaaaatcgg
      841 tgttggcgtc attttatcta aatcagctcc gacaacgctt gctgtaacct tagctaatga
      901 cctaaatatt acggcagtag gatttattcg aaatggtggc tttaatatat atagtcatcc
      961 agaacgtatc atcgactctg aacaataaca tattcattat agtgtgtcat aatgaacgat
     1021 attctacatg aatcaacaat ataaaagcac tatttttata agtataagta gctcaaaaca
     1081 atttaaaaag cagtaagttg atttctactc gaaaccagct tactgctttt ttattctaat
     1141 acaaacctat catcaatcgt taatttccaa tatcttcttt taatatttga taacaaaatg
     1201 attggtaaac ataaccttgc tttgcataca tatcttttgc tgtatcttca ccatctgcaa
     1261 ctaatatgat tggtttgtga ttttttgata tggcgtattc acctatcaac gattgaattg
     1321 tagatccaat tccttggtgc cgaaattgtt ctaatacacc aaatccatct aattcaatgt
     1381 aattttcact ttcaatgaca tctacaacgc caataggttc attatttaaa taagctacta
     1441 agcgtttaat cacatctttt tgataatgtt cccttaccat ttcttcatgt gcatctgcat
     1501 agtcttttcc aaatggcaaa ctaaactgat atgcaacttt taaataggca tctatatgcg
     1561 actcgtcaac gatggcaatt tcaacgtcag agttacgcgg caaattggca agcgcatcac
     1621 tttctatggc atacaattcc ataataccta gttcaaaatt catatcttta aacatagcca
     1681 tccaatgatt atcaagttgt tcattttccg ggaaactaaa cgcataatga tttgaacctt
     1741 gatttgtatg taattgatgt tgacgttcta agtctttcag ccatcgctct tgggtaggcg
     1801 tctttttata tatccatgtg ttgcttgtgt atcgttgtgg aaaagaaggc gttaaataaa
     1861 ttaactgctc gtcgtcattt tcaaatatag tgccgttaca atatatatct gacatactta
     1921 tactagccat tgacacctac tcctttcact tttataaatt tcaaagcaac ttattcaatt
     1981 actttattat cttaacttaa atatgattat tttaatccca taatttgacg aaactgcgag
     2041 tgattaagta aagctgtacc aattaaactt gctacaattg cttttaatat atcaccaggc
     2101 aaataagcta atgaaattga aatagctttc gtaaatggta tgttaataat catgcccata
     2161 atcaatgtac caataacatc taatgctata acaccaaaaa ctaaaatacc aacgaataaa
     2221 atccagaaat taatttcatt gatgaatcta tcacgaatcg caccaatcat gaatgctaca
     2281 actggatata atagtaaaaa ccctgctgaa ggacctgcga atacaccaat gccaccgcga
     2341 ccgcctgata acagtggcaa gccagcaact actaataata aaaagacgat aacacttaat
     2401 gtgccatatt tacgtcctaa aatcacacct gctaagaaaa taccaatgtt ttgaagtaca
     2461 attggtactg aagaaaatgg tagtggaatt accggtacca atcctaaaat agcgataatc
     2521 gctgtcatta aagctgtata tactaactgt tttgttgtca ttttttactt cctttcgtat
     2581 ttcatgacca ttattatagc accattgtaa actttaagaa acgaaaagtt tacaataagt
     2641 tgttctatat aaattaaaac cttaattatt ctacacagct gttaatttac ttttttagca
     2701 agtgcagtta tataatcaaa aaatgcgttg ttatctacat gatttatgac attaattttg
     2761 cgcccatctt tacactcaaa tgtcttacct tgacttggtc cataacttat tacatcgact
     2821 tttttctcaa ttgaatgaac caagtccttg ttaccaatat aagcagtcgt taaaacatcc
     2881 cataaaaagt aagtagaatt tgttataaag tgtgttaatg gtggtaccgc tgcataactt
     2941 actcctaaaa aatcaatgcc cgtatattgg cgttcattcg cccatctttg tctaacatct
     3001 aacgttaacg gtacttgatt cgtactttct aaagcaacca tatcaatctc tatatcgcta
     3061 tcaaaaacaa ttttaaccgc ttctggatcc caatatgcat tccattctgc agaaccatca
     3121 tgctcaggtt cttcaacatt tccttttggt aaaaaggtac cacccatcca aactaatttt
     3181 tctatatact gaacgataga tgaatctttt tgtagtgctt ttgctaaatc tgtaagcggg
     3241 cctgtaaata ataatgttac tttttctgat tgtctcttta aagtttgaat aatgtcttca
     3301 aaggcttctt tgtcgctcac atttgaagca acatgtttga ctggctcatt taaaattggc
     3361 aatgcgtcca taaaaaaggc atgcatacgc cattctttag gaaatggatt ttttcctcgt
     3421 tcatatgatg gcgcaacttg aatatcttca tttgaaaaac gattaataat ttttactgat
     3481 gcgctcaaag atggctctaa ataacaatca gcaccaattg tactgacccc tatcaattga
     3541 acgttttcca tttgtaataa taaaaataga gacactaaat catctacacc accatcatgg
     3601 ttaaaataaa ctttcttagt cactcgaaat cccccttaca tttcattttc cattaacaat
     3661 attatgtttt tataaaacgt tgttatatat ctttctacaa aatcataaca ttctgttgta
     3721 atccttttaa acctcttctt ttattgatag ccattccctg atatcccttc cataaagtat
     3781 gaactaatat aattatgata taacttatta ataaagagat acattgagct attttctcag
     3841 gtatactact ttagctataa taatactaat ctatcattaa ttaaattgaa cgataacaac
     3901 ggcacttatt attaaaaaaa atacaaatat ctaactttct agctataaaa ataagagcct
     3961 aatacaatat caaaagattg atatgtttca gactctcatt tcactaactt atatagctga
     4021 attattttgc agctttaatt aatttttctt ttaaatcttt acgcatgaaa tctaatgtat
     4081 aaggatcgtt gtaccagtat gtaccagcat caactttaac aatatgtcct tctttagtag
     4141 ctttcaaatt cttccacatg tttgttgatt catatcctgg tgtaggttta ccttcacttg
     4201 tactcacaat gtaatcacca gcatattttt caatttcttc ttgtttcact tcagcccaac
     4261 ctgcttttgc agttaacttt tgttgttctg gttgcatttt taaaccaaat gcttgatata
     4321 atacttctcc accacgaccc cagttatcgc cgtaagtgta taatttttta tcaaattcat
     4381 caaacaatga cactgttgca tcttgtccaa ttgctttttt aatttcttta ccgtctttag
     4441 cagttgtttc ttcccaatct ttcttccaag cttttacttt atcttcttta ccaacaattt
     4501 cccctaacat ttcttgttgt tctaaatatt tatgcttatt atagtcaaca actactgttg
     4561 gtgctacttt ttgatatttt ttaatatctt tgtcagtaga gtatacaata attaaatctg
     4621 gcttttcttt agcaactttt tctacatcgc catcaccaat ttttgtaaca cctttaaatt
     4681 tatcttttaa tactttgctt tgatcgactt gttgatttac agctacaatg tttgcaccta
     4741 attttttaag tccaccagca tatgttggcg caactactgc aatgcgttta gggtctttcg
     4801 gaatatctac cgttttgcca tcgtccattt tataagattt agtttcagct ttgttatttt
     4861 tttcaccttg gttcccacac gcagctaaca ctaataacat aattattaat ggtaataata
     4921 gttttttcat aatttcctcc tattgaaaat gattatcaat tattccaaaa ttatacaaga
     4981 ttgcatatac atttacaata gctagtgcta tttatttttt tattttcgca aaaaatttag
     5041 caaaatatct taacatccct ataaatcccc acaaaaaagg gtaaatccga acgtcaaatt
     5101 tcatctcttt atgccaatca acgatcgatt taccttttta aatttaaaat gtatctgtaa
     5161 tactcttagc aatatttgta tatgctgcat cttccatagg tggattgtgt aatcctgcac
     5221 gcatgtcacg atagtaacgt tgtaatggtc gattcatttc taagctttta gcaccaacta
     5281 ttctcatcgc taaatcaata acttcaagcc cttgattcat taccatcact ttactcgcac
     5341 ttgttggatt tcttatctga ctgtcatctt tatattgttg ataccctttt gctgtactcc
     5401 ataaaaattg tctcgcacat aacaatagcg tttccatttt ccctaaattt tgttgcacag
     5461 ttggcaatgt tgcaatcgtt ccttcaatac tattagggct atgttggatt gcaaagtcta
     5521 cagcataatt tctagcagct tgtgctatac ctaaataaca gcttggtata tgcaatatcc
     5581 aaccatttgg cgctttactt ttctctgttt ccactaaatg ttttaatggt acttttacat
     5641 catttaatat taaatcatga ctttctgtcg ctcgcatacc taatacgtcc caattatcgg
     5701 caatttcaac accaggcaaa ttcctgtcta ctaagaaaaa accaacactt tctaattctt
     5761 ctatataagc agcaacaata atatgtgtta gtgctttact cattgatgta tatgtcttca
     5821 caccatttaa aatatactca tcatcagctt taacagcatg tgtacttggt cttccccctc
     5881 ttgttggact acccatttca gcttcactaa ctgctctatt aactaatgca ccattattaa
     5941 tttcaacagc aaattgctcc aacatatctt gagaccataa tttctgttca taaatttgtc
     6001 ctacgacact cacatgccaa ccaatagata atgctgtggc accatcaagt tcgcctaaaa
     6061 atgattgcaa aataaccatg tcttctatgg tcgcaccttc accaccatat gcttttggta
     6121 acgttaattt tccataacct tctttaatta accattcaat atttttatat ggaaattggc
     6181 tatgttgatc attatattca gctttagcct taaatgtatc tctaattact ttgaattttt
     6241 caatccattt tctttgaatt tccgttgtta ttaaaaagga attatccata tcattcatat
     6301 tagtcacccc acacaacaca caatataaat tatttattcc tagtttaaca ctcgacttta
     6361 aaaataaaaa ggaacgtgct gtgaacaatt aaaagaaaac tgataattcg attaagtatg
     6421 cacttaattt tatccataaa aatacacctt aaactataca attcgctgta aagtttaagg
     6481 tgatattcaa acaatattag gtttgattta actatcaagc atctactttg tcatgtttaa
     6541 taccagcaaa taataaaatc catgtcacaa taataaatgg caatgttaat gcaggtaagc
     6601 caaacggttc aagcaatgtt gttgtaccta gttgcaccac tactgttaat aacacaccta
     6661 aaaaagtcga aatataaggg ttaatcgctg ttttaaatgt atatcctagt gcgattgcca
     6721 ttaatacaaa gttataaccg aataatccct gattaatatc atcataatta ccacctaata
     6781 gagctacaat gataaagctt aacaaactag ctataacagc taagagtgct gcttttcttg
     6841 atgctatcaa tatgccgatt aaaatacata cgccaccaat tacactcgct tcgataaata
     6901 cttgactgaa tccttcaaat aatgactgaa taaaatgtat tcgatcattg ttgctaaaat
     6961 taaccgtttc aatgttttga ggcattaact ttaacgatgt atctacaaat ttaacctgtc
     7021 ctgaaagtaa aattgtaaac caagtcacaa tgacaaaagg catcgtcagc atcggaactt
     7081 tatatggtct taaaacttct ctcactgcag cagcaactgg taacgttaat aaagttgcta
     7141 tcattgttat aacaatatct aatcctgact tatctaaaaa gattgttaac gcaatggcag
     7201 ttaacactgg attaaatccg gctaacccat cattaatctc tgcttcacta taattaatgt
     7261 aacgtgcaaa tgcataagct atgatactac ctacaatagc cgctaatcca attgtccaat
     7321 cggctacaaa taatcctatt aagataaata atcctgtcca tttattacta attaacacaa
     7381 cctgagaaat atttttcaaa agtacatcta ttatcttcaa atctttcacc tttcctttga
     7441 atcattactc attaatatac tactattcta tgaaaagtta tattaaaaac tattttggtt
     7501 tttacataaa tatattccac tatttgtttt cgattagatt gtttgcttca atttcggata
     7561 tgctaaaaat ataattttct aattacacat tgaactttgt atattacttg tttatcacaa
     7621 caattaggat taatataaat tttgtaaatt gtatctaata tgaaattggg ggttttaatt
     7681 tgcattttac acaacgagag caagacaaat taatgattgt agtggcggcg gaagttgcac
     7741 gtcgtcgtaa agcacgtggt ttgaaactaa atcatcctga ggcattagct ttaatcagcg
     7801 atgaattatt agaaggtgca cgcgatggta agaccgttgc agagttaatg agttatggta
     7861 gacaaattct aaacaaagaa gatgtcatgg atggtgtcga acacatgatt acagatatcg
     7921 aaatcgaggc tacgttcccc gatggtacta agttaatcac agtacatcac cctattgttt
     7981 aaaggaggca gtcaaatgat accaggagaa attattacaa aaagtacaga ggttgaaatt
     8041 aataaccatc atcctgaaac agttatcgaa gttgaaaata caggagatcg accaattcaa
     8101 gtgggctcac attttcattt ttatgaagca aatgcagcat tagatttcga acgtgaaatg
     8161 gcatatggaa aacatttaga tatcccagct ggagcagctg tccgatttga acctggggat
     8221 aaaaaagaag ttcaattagt tgaatatgct ggcaaacgta aaatttttgg ttttcgtggt
     8281 atggtcaatg gtcctatcga tgagtcacgt gtctatcgcc caactgatga aaatgatgaa
     8341 tatgcaggtg tattcggaga taacggtgct gaaaacgtga ataaaaaagg aggaaaaaga
     8401 tcatgagctt taaaatgacg caaaatcaat atacgagctt atacggtcca actgttggag
     8461 attccattcg tttaggtgat acgaatctat ttgctcaaat agaaaaagac tatgcggttt
     8521 atggtgaaga agctactttt ggtggtggta aatctattag agacggtatg gcgcaaaatc
     8581 ctcgtgtaac acgtgatgac gtgaacgttg cagaccttgt catttctaat gccgttatta
     8641 tcgattacga taaagtggtt aaagctgata taggcattaa aaatggttat attttcgcca
     8701 taggtaatgc cggcaaccca gatataatgg ataatgtcga cattattata ggttcaacaa
     8761 cagatatcat tgccgctgaa ggtaaaatcg tcactgctgg tggtattgat actcatgttc
     8821 attttattaa tcctgaacaa gcagaggtcg cattagaaag tggcattacg actcatattg
     8881 gtggtggtac tggtgcttca gaaggttcta aagcaacaac tgtaactcca ggtccatggc
     8941 atattcatag aatgttagaa gctgccgaag gtttaccgat taatgtcggt tttacaggta
     9001 aaggacaagc aacaaatcca actgcactca ttgaacaaat caatgccgga gcaattggat
     9061 taaaagtaca tgaagactgg ggtgcaacac catctgcttt gagtcatgca ttagatgttg
     9121 ctgatgaatt tgatgttcaa attgcattac atgcagatac tttaaatgaa gcaggattta
     9181 tggaagacac aatggctgct gttaaagacc gtgtacttca tatgtaccat actgaaggtg
     9241 ctggtggcgg tcatgcgcct gatttaatta aatcagctgc attttcaaat attttacctt
     9301 catctacaaa tccaactttg ccttatacac ataatactgt agatgaacat ttagatatgg
     9361 taatgattac tcaccattta aatgcggcta ttcctgaaga tatcgcattc gcagattcac
     9421 gtattcgtaa agaaacgatt gcagcagaag atgttctgca agatatgggt gtattcagta
     9481 tgattagttc cgattcacaa gcaatgggcc gtgtaggtga agtaattaca cgaacatggc
     9541 aagtagcaca tcgcatgaaa gaacaacgtg gtcctttaga tggtgatttt gaacataatg
     9601 ataataatcg catcaaacgt tatatcgcta aatatacaat taacccagca attacacatg
     9661 gtatttctga atatgtagga tctatcgagc cgggcaaact agctgacatt gtcttatggg
     9721 acccaatttt ctttggggtt aaacctgaat tagttgtaaa gggcggatta attaactctg
     9781 ccgtaaatgg cgatgcaaat ggttctatac ctacatctga accgatgaag taccgtaaaa
     9841 tgtatggtca atacggcgga aaccttacaa gtacgtcaat gacattcgtg tctaaaactg
     9901 cttatgaaaa tggtatcaat cgtgcattaa atttaaaacg catggtgcgt ccagttaaaa
     9961 atattagaca attatctaaa gcagatatga aaaataacag cgcaacacct aaattagacg
    10021 ttgatccaca aacatatgaa gtatatgtag atggagaaaa aattacaagt aatgcagcaa
    10081 ctgagttacc attaactcaa agatacttct tattctagga ggaacataga atgattgttg
    10141 aagaaatcca aggcaatatt gccaatttgt caaattcaga aaaacaaaag cacgtcgaaa
    10201 aagtatacct tgaaaattca gatcttgtta aacgtattca aagagtcgtt acagaccatg
    10261 gcactgaaat aggcattcgt ttaaaacaac ctattgactt acaatatgga gatattttat
    10321 acgcagatga ccataatatg attattgtgg atgttaattc agaagatctt ttagtgattc
    10381 aaccaagaac attgcaagaa atgggagata tagcccatca attaggaaat cgtcatttgc
    10441 cagcacaatt tacagagact gaaatgctag tacaatatga ttatttagtt gaagatttat
    10501 taaaaagttt aggcatccca tatgttcgag aagaccgtaa agttaataag gctttcagac
    10561 atatagggca ttctcatgat tgatcataca cacttaagat tatttcagtt ctgtgattca
    10621 cagtttccaa caggtgcttt cagtcattca tttggtcttg aaacatatat tcaacgaaat
    10681 ataattcatg acgatcatac ttttattgct tggttaaaaa tgtttttaca agagcaactt
    10741 acctattctg atggtttagc catgcgttta gtttatgatg cattggaaaa tgatgataca
    10801 caaaaagtat tacacataga taaactgatg tttgttcaaa acttacctaa agaaacacgt
    10861 gttggtgcaa aacaaatggg gactcgcatg gttaaattgg ctttagaact ttataatagc
    10921 ccatggattg cttggtatca tcaacaaatg caagataaga aagcaaagtt aaatccagct
    10981 atttgtttta ctatgctagg ccatcattta ggtgtagata ttgaaacgat tattgattac
    11041 tatttatatc aaaatgtttc aagtttaact caaaatgctg ttcgtgctat tccacttgga
    11101 caaactgctg gtcaaaagat agttactcac atgattcctt atatagaagg aacaagaaag
    11161 cagattttcg aattaaaaga agcagatttt ggcatgacag cgcctggttt agaactaaat
    11221 caaatggcgc atgagaacgt caatgttaga attttcatat cataggaggt tataattgtg
    11281 gcaaatccga ttaaaattgg tattggtggt cctgtaggtg caggtaaaac acaattaatt
    11341 gaaaaagttg taaaacgtct ttcaaaagaa atgagtatcg gcgttattac aaatgatata
    11401 tatacaaaag aagacgaaaa gatattagta aattcaggag ttctacctga aagtcgtatc
    11461 attggtgttg aaactggtgg atgtcctcat actgcgattc gtgaagatgc atctatgaac
    11521 tttgcagcaa tagacgaatt attagaacgt catgacgata tagaactaat tttcatagaa
    11581 tctggtggcg ataacttagc agcaacattt agcccagaac ttgttgactt ttcaatatat
    11641 attatcgatg ttgctcaagg tgaaaagatt ccacgtaaag gtggtcaagg tatgattaag
    11701 tcagatttct ttgtaattaa caaaactgat ttagctccct atgtaggtgc atcattagaa
    11761 caaatggctg aagatactaa agtatttcgt ggtaaacgtc catttacttt tactaactta
    11821 aaaaccgacg aaggtttgga tgaagttatc gattggattg aacgcgacac tttactcaaa
    11881 ggattatcat aatggatgaa caacaatgga ctgggcaact tgatttaaca gtgtttttcg
    11941 atggcaatcg atcagtatca agagatattt tctttgaaaa agcacttaaa gtgatacgtc
    12001 cagtttatct aaatcaatct accattccta cattttatat agtaaatgta ggtggtggct
    12061 atttagatgg agatcgttac cgtatgaatg tgaatgtcga agataacgct aaagtgacat
    12121 tgacatctca aggtgcaaca aaaatataca agacaccttc caatcatgtt gagcagtatc
    12181 aaacttttaa tttgaaagat aacgcatatt tagaatatgt cgctgatcca atcatcgcat
    12241 atgaaaatgc taaattttat caacacaata cgttcaatct caataattct agttcattat
    12301 tttatactga tattttaact cctggttatt caaaaactgg cgaagccttc aagtatcaat
    12361 atatgcattt aataaatgaa atttatattg aagatgagtt agtcacatat gataatttat
    12421 tattgaatcc taataaacaa tcaatcaatg aaataggtta tatggaacat tactctcatt
    12481 atggctctgc ttacttcata catgaagatg ttaaccaaaa actaatcgat tcagtttatg
    12541 aaaccattag ttcttatagc aatacattcg attgtcgtgt tgctatttct caattgccta
    12601 cacatggttt tgcagttaga atttttgctt atcgcacaca aattatagag aaaatacttg
    12661 gaaccatcca aagctatatc gcagaaaata tttatgatcg taaacttgat tttctaagaa
    12721 aatattaaaa taaaaagact agtgtacctt gtttcaagct taataagctg aaataaggta
    12781 tggctagtct ttaactttaa ttaaagcatc ttcaatattt aaattttaag ttaattttat
    12841 gtcccaatgc tgaataaata acttcgtttt aataaattgt tatcgttcaa tgttattaaa
    12901 cgcaattaac cttgatttaa tttttaatgt attcttctaa ttctgaaatc agtttttgaa
    12961 tatttgcttt ttgtgtatct gtaacataaa caataactgt tctttcgtct tgtaaacttc
    13021 ttttctttga taacaatttt aaatctttta gcttttgtaa agctttagtt aaatagtaag
    13081 gtttgaactc tgagcactta gcaatctctt tagatgagat ttcgttagac tcacttctta
    13141 aaatatgatt taaaatataa atttcttcat agttcaaatt gaacttcttt tttgtatctc
    13201 tgaaaaactt cttaacttga aatgttgcgt tgactaaatc attaatgtca ttaattttac
    13261 tcattattaa accactcctc tgatgcacat cttgttgtag caaggttcac atgtaattta
    13321 ttaaatcgaa actctatcca actatgttat aaagttcatt ctaaacaaat aagtataaaa
    13381 attcaatatt tttattacta gaatatggtt aaatacatt
//
LOCUS       DAHHYJ010000074        13197 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000074 DAHHYJ010000000
VERSION     DAHHYJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.074"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(181..1167)
                     /locus_tag="NBK22_001585"
     CDS             complement(181..1167)
                     /locus_tag="NBK22_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD2414774.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNNRVNFSGDEKMALLKISSSIKD
                     IFYDGTFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            1637..2395
                     /gene="map"
                     /locus_tag="NBK22_001586"
     CDS             1637..2395
                     /gene="map"
                     /locus_tag="NBK22_001586"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCD2414775.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            2612..2998
                     /locus_tag="NBK22_001587"
     CDS             2612..2998
                     /locus_tag="NBK22_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414776.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            3013..3714
                     /locus_tag="NBK22_001588"
     CDS             3013..3714
                     /locus_tag="NBK22_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCD2414777.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            3711..4754
                     /locus_tag="NBK22_001589"
     CDS             3711..4754
                     /locus_tag="NBK22_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD2414778.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            4744..5373
                     /gene="vraR"
                     /locus_tag="NBK22_001590"
     CDS             4744..5373
                     /gene="vraR"
                     /locus_tag="NBK22_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCD2414779.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(5485..6702)
                     /locus_tag="NBK22_001591"
     CDS             complement(5485..6702)
                     /locus_tag="NBK22_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCD2414780.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIVFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDNNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(6980..7255)
                     /locus_tag="NBK22_001592"
     CDS             complement(6980..7255)
                     /locus_tag="NBK22_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD2414781.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(7262..7726)
                     /locus_tag="NBK22_001593"
     CDS             complement(7262..7726)
                     /locus_tag="NBK22_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCD2414782.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            7873..8073
                     /locus_tag="NBK22_001594"
     CDS             7873..8073
                     /locus_tag="NBK22_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCD2414783.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     RERLSPSEVTAIADALGQLRHD"
     gene            8076..9332
                     /locus_tag="NBK22_001595"
     CDS             8076..9332
                     /locus_tag="NBK22_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCD2414784.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHELKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGEAVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            9421..9951
                     /locus_tag="NBK22_001596"
     CDS             9421..9951
                     /locus_tag="NBK22_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD2414785.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(10211..11362)
                     /gene="yfkAB"
                     /locus_tag="NBK22_001597"
     CDS             complement(10211..11362)
                     /gene="yfkAB"
                     /locus_tag="NBK22_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCD2414786.1"
                     /translation="MNSVKLKQPVSIYNDPWESYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPQPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRTAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            11586..11747
                     /locus_tag="NBK22_001598"
     CDS             11586..11747
                     /locus_tag="NBK22_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414787.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            11878..12393
                     /locus_tag="NBK22_001599"
     CDS             11878..12393
                     /locus_tag="NBK22_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD2414788.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            12734..>13197
                     /gene="sgtB"
                     /locus_tag="NBK22_001600"
     CDS             12734..>13197
                     /gene="sgtB"
                     /locus_tag="NBK22_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCD2414789.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKV"
BASE COUNT         4666 a         1945 c         2302 g         4284 t
ORIGIN      
        1 agagaaattg gattcccaat ttctacagac aatgcaagtt ggggtgtggg ccccaacaca
       61 gagaatttcg aaaagaaatt ctacaggcaa agcgagttgg ggtgggacga cgaaataaat
      121 tttacgaaaa tatcatttct gtcccactcc catggtgcca attagcataa ggtacttaaa
      181 ttaagcatat ctgctgtcta gcagtcgata aatcattaga acttcgtata gtatatgact
      241 tttaatttga ttttcaccac taatttcaag tgcttttata gtcgaacgta aagtttctac
      301 agaatcatct tctctcttaa aagtaccatc ataaaatata tctttgatgc tactactaat
      361 ttttagcaat gccatttttt cgtcacctga aaagttaaca cgattatttt taggcaagta
      421 aatgatattt gataaatgag tgataaacaa acgattcgta tatgcacgtt tagttaattg
      481 attgagtaat ttccaatcac attctttttt cttatgatag cttaattcat cacgttgata
      541 acttattaac gtttcaactt ggttgtttaa attgaaaata tttttatatg ctttttcgct
      601 tttatcagat tgcagtcttg ataagataag ttcttggcag cgattgtaaa ataatttata
      661 catcaaggca tctgtcttac ttaatttttc ttcgacctga ccataatact taggtggaaa
      721 caccatgaag ttaattaaac ctgatgtcac gagtccaata attgctgtca atgttcgaga
      781 caaaaagttg aatatgtagg catcatgaat acctggaatc atagctaatg atgttagtac
      841 agcgacattc gtaccaactt gcaatttgag ttttgtacag aataaaatcg tgaacgttgc
      901 actcaatgca tatgtaaaag gtgattgatc gccgaataaa tatgtaaata atactgcaaa
      961 gcctgcacca attaccgtag caggtaatct acgataacct ttaataagtg atgccttggc
     1021 agttggttca attgtgacta cagctgttaa aatggcatag atgggtgtta aatctagtgc
     1081 catacaaaag acagctgtta aaaaaatggc aataccagtt ttaattgttc tggcaccaat
     1141 taaatgttta taccattgat cgttcatttt ttaacctcta atcatcgtaa aatcttagcg
     1201 agcgctttat aataatagta tcgtacattg gaaaagttca tgtatgtaaa atatttgaaa
     1261 taatcatact taagcattac tttgattttc atatacatta atcaaagttc tgggctccac
     1321 aatgttatat tgttagtagt tgactgaaat aaaatgcact tgtaacaagc atttttcaat
     1381 tatagtccgg ggccccaaca cagaagctga cgaaaagtca gcttacaata atgtgcaagt
     1441 tggggtgtgg gccccaacac agagaatttc gaaaagaaat tctacaggca atgcaagttg
     1501 gggacgataa agaaatattt tttctttatc acactatgtc tcactcactt tccaaaatac
     1561 taaagtaaca tctttagtat atcaaagaat ttttgctata ataagttata attatataaa
     1621 aaaggaacgg gataaaatga ttgtaaaaac agaagaagaa ttacaagcgt taaaagaaat
     1681 tggatacata tgcgctaaag tgcgcaatac aatgcaagct gcaaccaaac caggtatcac
     1741 tacgaaagag cttgataata ttgcgaaaga gttatttgaa gaatacggtg ctatttctgc
     1801 gccaattcat gatgaaaatt ttcctggtca aacgtgtatt agtgtcaatg aagaggtggc
     1861 acatgggatt ccaagtaagc gtgtcattcg tgaaggagat ttagtaaata ttgatgtatc
     1921 ggctttgaag aatggctatt atgcagatac aggcatttca tttgtcgttg gagaatcaga
     1981 tgatccaatg aaacaaaaag tatgtgacgt agcaacgatg gcatttgaga atgcaattgc
     2041 aaaagtaaaa ccgggtacta agttaagtaa cattggtaaa gcggtgcata atacagctag
     2101 acaaaatgat ttgaaagtca ttaaaaactt aacaggtcat ggtgttggtt tatcattaca
     2161 tgaagcacca gcacatgtac ttaattactt tgatccaaaa gacaaaacat tattaactga
     2221 aggtatggta ttagctattg aaccgtttat ctcatcaaat gcatcatttg ttacagaagg
     2281 taaaaatgaa tgggcttttg aaacgagcga taaaagtttt gttgctcaaa ttgagcatac
     2341 ggttatcgtg actaaggatg gtccgatttt aacgacaaaa attgaagaag aatagttcaa
     2401 catatactaa gactaaagta tgaacatcat ttagttccgg agcctattca tattggtttc
     2461 ggaactgttt tataataatt aagaacacaa tcaatgcgtc atttcaaaaa tatgttgtaa
     2521 taaagtagtt tttaagcaaa catatcatcg acatcaacga agatacatag cgcatttggt
     2581 attttaaaac ttattataaa aggtgatagt tatgaactat gttgaacgtt atattgaaca
     2641 gtttttgaga gcaacagtaa gaaataatat caagcactac cttttaatgc tagatgaaaa
     2701 aatgaaaaat ttagatgatt atatgcgtta tttaattact aaaaaagaac aacttagcaa
     2761 gttaattgac agtctaatgc taacattaga aaataaatat attgatattg ctgaagcatt
     2821 tcaaattcaa tgtgcaagag aaatcaataa tcaagaaatt gaaaatatta aatcagagtt
     2881 gaataaagtc gaagcatatt atgcacaaat tgaaactcaa attcaacaaa cttcaactga
     2941 aaaaatagca acagaaaaaa catcgtatct aataaattat atgaacgctg tggcatagaa
     3001 aggcggcgaa acatgacaca caaatatata tcaacgcaaa tgttgatcat ttttactgca
     3061 ttaatgatta ttgccaattt ttactacata ttttttgaaa aaattggctt tttactcgtt
     3121 ctattattgg gatgtgtatt agtttatgta ggatatcttt attttcataa aatacgtggc
     3181 cttttggcgt tttggatagg cgcgctatta attgcattca cattattgtc taataagtat
     3241 acaatcatca tcttgttcgt ctttttatta ctacttattg tgcgttattt aatacacaaa
     3301 tttaaaccaa agaaagtagt tgcgacggat gaggttatga cttcaccatc ttttattaaa
     3361 caaaagtggt ttggtgagca acgtacacca gtttatgtat ataaatggga agatgtccaa
     3421 attcaacatg gaattggcga cctacatatt gacttaacaa aagctgcaaa tattaaggaa
     3481 aataatacca ttgttgttag acacatttta ggtaaagtgc aggttatatt gccggttaat
     3541 tacaatatta atttacatgt agctgctttt tatggaagta cttacgtgaa tgaaaaatca
     3601 tataaagttg aaaataacaa tattcatatt gaagaaatga tgaaaccgga taattataca
     3661 gttaatatct acgtatcaac gtttatcgga gacgtagagg tgatttatcg atgaaccact
     3721 atattagaac aattggttca atgctcatct tagtatatag catgctagct gcatttctat
     3781 tcatcgataa agtttttgta aatatcatct attttcaagg tatgttttat acacaaatat
     3841 tcggaatacc agtcttttta tttttaaatc tcatcatcat attattgtgt attattgttg
     3901 gttcggtact cgcatacaaa atcaatcagc aaaatgattg gattaagacg caaattgagc
     3961 gttcaatgga aggcgaaaca gttggcatta atgatcaaaa tatagaaata tatagtgaaa
     4021 cgttagattt ataccataca ctcgtacctt taaatcaaga attgcataag ttgcgactta
     4081 aaactcaaaa tttaaccaat gaaaattata atattaatga tgtgaaagtt aaaaagatta
     4141 ttgaagatga acgtcaaaga ctggctcgag aacttcacga ttctgttagt cagcaacttt
     4201 ttgcggcaag tatgatgtta tctgctatca aagaaacgaa gttagaacca ccattagacc
     4261 aacaaattcc gattttagag aaaatggttc aagattcgca gttagaaatg cgtgctttgc
     4321 tgttacattt aagaccgctt ggtttaaaag acaaatcttt aggtgagggt attaaagatt
     4381 tagttattga tttacaaaaa aaagtgccaa tgaaagttgt gcatgaaata caagatttta
     4441 aagtgcctaa aggtattgaa gatcatttgt tcagaattac acaggaagca atttcgaata
     4501 cattgcgtca ttcaaacggt acaaaagtga cagtagaatt gtttaataaa gacgattatt
     4561 tattgttgag aattcaagac aatggtaaag gttttaatgt tgatgaaaaa ttagaacaaa
     4621 gttatggact taaaaatatg cgtgaaagag ctttggaaat tggtgcaacg ttccatattg
     4681 tatcattgcc agattcaggt acacgtatcg aggtgaaagc acctttaaat aaggaggatt
     4741 cgtatgacga ttaaagtatt gtttgtggat gatcatgaaa tggtacgtat aggaatttca
     4801 agttatctat caacgcaaag tgatattgaa gtagttggtg aaggcgcttc tggtaaggaa
     4861 gcaattgcca aagcccatga gttgaagcca gatttaattt taatggattt acttatggaa
     4921 gacatggatg gtgtagaagc gacgactcag attaaaaaag atttaccgca aattaaagta
     4981 ttaatgttaa ctagttttat tgaagataaa gaggtatatc gtgcattaga tgcaggtgtc
     5041 gatagttaca ttttaaaaac aacaagtgca aaagatatcg ccgatgcagt tcgtaaaact
     5101 tctagaggag aatctgtttt tgaaccggaa gttttagtga aaatgcgtaa ccgtatgaaa
     5161 aagcgcgcag agttatatga aatgcttaca gaacgagaaa tggaaatatt attattgatt
     5221 gcgaaaggtt actcaaatca agaaattgct agtgcatcgc atattactat taaaacggtt
     5281 aagacacatg tgagtaacat tttaagtaag ttagaagtgc aagatagaac acaagctgtt
     5341 atctatgcat tccaacataa tttaattcaa tagttcatat cgaattaaga aaagttactt
     5401 acgccaatca caatataaca tcaaatagac acaaaaaaca gaggtcttcg caatcattag
     5461 cgaaaacctc tgttttgttg ttgtttagtc ttttttaagc ttatcaacga ttttgtcttt
     5521 tatattttta tcatcttctg gataggtatc actaggatgt tcatcaacat gatacgtacg
     5581 ttgatgtgtt gcattattat cctcatcata ttcgtaccta gtgttctcat tataatgatt
     5641 tttattattg tcatcatgtt ctaatgctgc ttcttcaggt gttttacctt taattacact
     5701 acgctgatga atgattgcat tgatttcagc accgacaatt ataataaaac ttgtgatata
     5761 taaccatagt aacaaaatga tgatacccgc gatactgcca tatgttttag aatagttacc
     5821 aaaatttgaa atataccaac caaaaccaaa tgaaccagct aaccaaataa ttgaagtaaa
     5881 tactgcacct ggtaatactg acttaagctt cgttttaacg ttaggtgcaa ccgaatataa
     5941 cacgataaat acgataaata taataatgat tggtaacaca attctaataa ggttaaaaat
     6001 ccatttcact tgttcgtcaa atccaagtgg accgaatagg aaatgactaa ttacagaacc
     6061 aagcgttggt aatgctagag caactacaaa cacaacgccc ataactacag tgaagacaac
     6121 acttagtagt tttaatacga ttccattacg gctatcttct acatcgtaag caacattgaa
     6181 agaattcata attgcagtca ttccatttga agctgaccaa attgctaaaa tcaaaccgat
     6241 agataataag ccaccactgg agttttgagt tatatcacca attacactct taattagagt
     6301 agatgtttca gcgggtgcat tgcttaacat attagtaatt tgactctgct taatattgaa
     6361 aaatggtaat aatgttaata ggaaaagcaa catagggaac atagcaagta cgaaatggta
     6421 agtcatttgt gctgctagtc ctgaggcatc atcttttcct atgcgataga tgagatatga
     6481 taagaaatta gagtctttct tatattttgc aggtttattt aatcttgata agaagaaaac
     6541 ctgatcatct tttttaggtt tcttagattg gaattgttga ggttcaatat atgtacgatc
     6601 aacatcaatt ttgggattac ttttattttt cttttttgac tcctcttgcg cttccttaac
     6661 agaattaagg tattttgaag aagagtgatc ctttttcgac atgtgcactc cttaaccttt
     6721 ctctatgtaa tgttttataa agtaactatt agtatttatc caattataaa aaaactaaac
     6781 aatgatatgc tttaaaaatg ataatattat agaaattaat tgatttaaat ctttcatagg
     6841 ctattttgat ttaaaattaa aacaaaggca atactgaaac aatgtagttt ggccaatcat
     6901 tgtattatca tttaataatg ataactatga aaggcgtaca ccaaattgtt aagtattgcc
     6961 ttataaaaac aaaatcatct tagcgttgat tttttctatt aacaaatgtt tctttcgcat
     7021 cttttaatga acgttctaat tcaggattat tacgacgaat ttcttcaaca acatctttcc
     7081 agtataaaac ttcgtcttta atcttgctga ctttagaagg cttacgagtg cggttaccat
     7141 tttttgcatc tttaactgat tgaactaaag cttgacgtgt agatttatca gctaaactaa
     7201 ttgcaccacc aattacggca ccaattaaaa taccaggaac aaatttattt tccataaaaa
     7261 actacccctc tttcaaattt gcatctttta cgatgtagtc tattaaatta tcacaagatg
     7321 ataataccat gtcgtataca ccttcaaaat tattcgtgta gtatggatct ggtacatcac
     7381 tctcttccat attactaaat tctaacagtt tgaacaattg tcccttaaga ttaggattga
     7441 tagatttaat attatcaacg ttactttgat ccatagccac aatgtaatca aaatcatctg
     7501 tcgcttcgaa taattcacta atcatgccat caaatggaat attgtgtttg ttgagaattt
     7561 tttgtgtacc ttcatgaggc ggctctccta aattccagct accagtacct cttgaatgta
     7621 ctttaatatc atgaatattt ctgtctttaa gtctttgtcg catgattgct tctgccattg
     7681 gagaacgaca tatattgcca agacagacaa atgctacatc taccattttg attcctccaa
     7741 actatgtagt tatatcccca ttttatagcg actttaaaca ataagaaagc agattatata
     7801 aaattctatt aaagtttatt aaattgtgat actttgataa cataactatt attagaggtg
     7861 aacattgtgg ctatgacaaa tgaagagaaa gtattagcta ttagagagaa gttaaatatt
     7921 gttaatcaag gattattaga tcctgaaaaa tataaaaatg caaatgaaga agaattaaca
     7981 gatatatatg attttgttca atcaagagaa agattgtcgc caagtgaagt gacagctatt
     8041 gctgacgctt taggacaatt gcgacacgac taggagtggg aattttgact aattacaaag
     8101 aaaagttaca acaatacgct gaactattag ttaaggttgg tatgaatgtt caaccaaaac
     8161 agccagtatt tattagatca tcagtagaaa cattagaatt aacgcattta attgtagaag
     8221 aagcatatca ttgtggcgct tcagatgtac gtgttgtata tagcgatcca accttaaaac
     8281 gcttaaaatt tgaaaatgaa tctgtggaac attttgctaa tcatgagtta aaatcatatg
     8341 atgttgaagc gagaatggat tacgttaaac gtggcgctgc taatttagca cttattagtg
     8401 aagatcctga tttaatggat ggtatcgata gccaaaaatt acaagcattt caacaacaaa
     8461 acgcacgcgc atttaaagga tatatggaaa gtgtacaaaa aaatcaattt ccatgggtcg
     8521 tagctgcttt tccatctaaa gcttgggcga agagagtgta tcctgaacta tctgtggaag
     8581 aagcatatat taagtttatt gacgaggtat tcgatattgt gcgaattgat ggtaacgatc
     8641 cagttgaaaa ttggcgtcaa cacatcgcta atttaagtgt ttacgctcaa aagttacaac
     8701 aaaaaaatta ccatgcgttg cattatgtat ctgaaggtac tgatttaaca gtaggtttag
     8761 cgaaaaatca tatctgggaa gatgctacaa gttatgttaa cggcaaggaa caagctttta
     8821 ttgctaatat accaactgaa gaagtattta cagcaccaga tcgaaataga gtagatggat
     8881 atgttactaa taaattgcct ttaagttata atggcacaat tatcgatcaa tttaaattga
     8941 tgtttaaaga tggagagatt attgattttt cagctgaaaa aggtgaagcg gtattgaaag
     9001 atttaatcaa tactgatgaa ggttcaagaa gattaggtga agtagcatta gtacctgatg
     9061 attcaccgat atcaaatcgt aataccattt tttacaatac tttatttgat gaaaatgcag
     9121 catgtcattt agcgattgga tctgcttacg cctttaatat tcaaggtgga acggaaatga
     9181 ctgttgagga aaagattgca agcggattaa atgattcaaa cgtacatgtc gatttcatga
     9241 ttggtagtag tgatttgact atttatggca tattcgaaga tggttcaaaa gaactagtat
     9301 ttgaaaatgg aaattgggca tcaacatttt aataaatgta ttttgaggtg ctgagtagga
     9361 aatgaaacat gtatttaaag gtcaaatacg tgattaaagt atagattggg agataaaata
     9421 atgacaaatc aggacagacc aatgaaatct atgtcagaat caaaatgtta taaaaacaga
     9481 caagttttcc ctcaagatac gaatcaccat catacaatgt ttggtggtac attgatggct
     9541 aatattgatg aaattgcagc aatcacagct atgaaacatg ctggtgcgca agtagttacc
     9601 gcatctacag actcagtaga tttcttaaag ccgattaaaa caggggacat attacaatac
     9661 gtagcgatgg tttcatacgc tgggactagt tcaatggaag tggtcgttca aattagaatt
     9721 gacgatgtat ttaataacaa gcatgattta gctgcattaa gttacctaac atttgttgcg
     9781 ttagatgatg aaggcaaacc taaacatgta cctggtgtat atccagaaga tgatgttgaa
     9841 aaatggtttt atgatacagc accacaacgt gttgaacgaa gaaaagcacg tcgcattgaa
     9901 agtaaacaaa caattgaata tttagctcaa gtgcaacata ttagagacta aaaatataaa
     9961 gacagattgt taaggatgaa ttgaactgtt atgttcgaat attccaccta caatctgtct
    10021 ttttgattat gcacgtttat gcttttagtt aaaatttaac ccttttatta tagactatta
    10081 gtcctattaa atgaccttta ttattaaagt agagtactgc cagcaattaa ttgtttttta
    10141 ttttataata gcattaaaat atgcggagac tacttatcga taaagtgctt ctccgcataa
    10201 ttaagagttt ttaaaattgt ataatttgtg gttgtttgtg catatgacgc tcattatctt
    10261 taaaatccat attcggatag tacatatttt taacaagaac atttggtcct aaacaactaa
    10321 actcggaaca atgacaattt aatgatttag caagatcaga ggataaccat ttatcaaata
    10381 catctgttaa tttatctttt tgtatattcg aaattgtacc tgtttcatct ccgaaatcag
    10441 ttacgattac attacctgta aatacattga catttaaacg actacggcca tccgggtcat
    10501 ttctagtcgt tacattttta gcagttctta aacgtgatag taacttttga tcttcatcat
    10561 cttttaagca tggaaacaca ggcaaagtac caaataacat ccaaatatct tcatctctga
    10621 aatccaatat atcatgaatt gtctttttca tttccgctag agttaacaca tttaattgac
    10681 ttgcaaagtc agctggatac atagggtgaa tctcgtgtct gctacatttc atatcatgaa
    10741 cgacttcttg atgtattttt cgtaaatgtg gtagcgtact ttgattgagc attgtttccg
    10801 cagatacaaa cattccttgt tctgataatg tacgtgcatt cgaaatcatt tgttcatata
    10861 attttaactt agcttttaac ggtggttgct tcttcattgc gccaaagcca acatttgcga
    10921 attcatcagt tgttccccag ttatgtgaga tatgcataac atcgatatat tcagcaatat
    10981 ctaaataacg atcttgaggc aatgttaggt ttgaattcat ttgtgtatat atacctcgat
    11041 gatgtgcata ctttaataga ggtttaacaa catttctaat agacttttta gaaaacattg
    11101 gttcgccacc tgtaattgac atcgttcgca gatttggaat ttcatcaagt ctacgataaa
    11161 ttaagtccat atctaaaggc tgggggtcga cagtttgtaa agtataacca actgcacagt
    11221 ggctacaacg catattacaa agatttgtag ttgtaaattc gatgttactt aaagttaatt
    11281 ggccatgttc tttaacatcg ttatatgatt cccatggatc attgtaaatg ctaacaggtt
    11341 gcttcaattt gacgctattc atcgtcttaa acttccttct ttctatttgg ttcatatcgg
    11401 tatgatacca cttcgtatta tcactcgtaa caaattatct cttaatttta gttaattgaa
    11461 aaggggattt ttaaaagtca aaataatttg aatgtcataa atatagctaa gattatatcg
    11521 ctttaacgca atttctgtta ggataataat gaaaaagaaa acaaaataaa aggagttaac
    11581 tttttatgaa tgaacagcaa acaatcgaac agataaaagc gcgtttaaat aagtttattg
    11641 aggatatcga tcatgtaaat cctgatgaag tacgtgttga agatatagat gaatggattg
    11701 gattgttaga tcagcttgaa gaaaaggtta aattagtatc taagtaaatg ccacataaga
    11761 tgtgttaggg ttagatatcg cgacagtcgc ttaataattt ttatatcttg tatgtttaat
    11821 cactgattta cttgtctata gtaattatat caatgatata tagaaaggtg attttcaatg
    11881 actaaaaaag tagcaattat tctagcaaac gaatttgaag atatagaata ttcaagccct
    11941 aaagaggcat tagagaatgc aggctttaat actgtagtga ttggagatac tgcaaatagt
    12001 gaagttgttg gtaaacacgg tgaaaaagtt actgtcgatg taggcattgc agaagctaaa
    12061 ccagaagatt atgatgcatt attaattcct ggaggatttt caccagatca tttacgtgga
    12121 gatacagaag gtcgatatgg cacatttgct aaatacttta ctaaaaatga tgtaccaaca
    12181 tttgccattt gtcatgggcc acaaatacta atagatacag acgatttaaa aggtcgtacg
    12241 ttaacagcag tattaaatgt acgcaaagat ttatcaaatg caggcgcaca tgtagttgat
    12301 gagtcagtag ttgtagacaa caatattgta acaagtcgag taccagacga tttagatgat
    12361 tttaatcgag aaatcgttaa acaattacaa tagaagtatt gtggttatcg attgttaaaa
    12421 tttatatatt ttcgtttgaa atgaaatatg gattttagtt ttaataaatg aataacaaat
    12481 aaaaggtcgt ctctattggc atttaatagg gatggtcttt tatttttaca aaatataaca
    12541 atatttgaca tgattaatat ttaaaattgc aatgatttat ctataatttt ttcaaaaaac
    12601 aaaaaagtaa gtatgatggc gtatacgatt gtaagccctt ggactgattt tcccaaaaag
    12661 tgtagcctat ttgtgatagt actggtaaac tcaaggtata tactaagtga gttttaaaag
    12721 aaggagcaaa cgcatgaaaa gaagcgatag gtactcaaac tcaaatgaac attttgagca
    12781 tatgaaacac gaacctcact ataatacgta ttatcaacca gttggcaaac cgccgaaaaa
    12841 gaaaaaaagt aaacgaatac tattgaaaat attattaacc attctaatca ttatcgcatt
    12901 gtttattggt atcatgtatt ttttatctac acgcgataat gtggatgaac taagaaaaat
    12961 tgaaaataaa agtagttttg tgtcagctga taacatgcca gagtatgtta aaggtgcctt
    13021 tatttcaatg gaagatgaac gattctacaa tcatcatgga ttcgatttga aaggtacaac
    13081 tagagcttta ttttcaacga ttagcgacag agatgtgcaa ggtggtagta ccattacaca
    13141 acaagttgtc aaaaattatt tttatgataa tgatcgttca tttactagaa aagtaaa
//
LOCUS       DAHHYJ010000075        12975 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000075 DAHHYJ010000000
VERSION     DAHHYJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12975
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.075"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            114..1154
                     /locus_tag="NBK22_001601"
     CDS             114..1154
                     /locus_tag="NBK22_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD2414790.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            1168..2235
                     /locus_tag="NBK22_001602"
     CDS             1168..2235
                     /locus_tag="NBK22_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCD2414791.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     EPVVKITEEQQFKCHIDTIIKKQVPVCCFTFGIPSESIIKRLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMVGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIGEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            2365..2448
                     /locus_tag="NBK22_001603"
     CDS             2365..2448
                     /locus_tag="NBK22_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414792.1"
                     /translation="MIIIKRKHQAEDRRKIMIKQKHQIKVR"
     gene            2496..3344
                     /locus_tag="NBK22_001604"
     CDS             2496..3344
                     /locus_tag="NBK22_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCD2414793.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPICVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            3357..4184
                     /locus_tag="NBK22_001605"
     CDS             3357..4184
                     /locus_tag="NBK22_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCD2414794.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFITTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            4211..5530
                     /locus_tag="NBK22_001606"
     CDS             4211..5530
                     /locus_tag="NBK22_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD2414795.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY"
     gene            5614..6531
                     /gene="lipA"
                     /locus_tag="NBK22_001607"
     CDS             5614..6531
                     /gene="lipA"
                     /locus_tag="NBK22_001607"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCD2414796.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            6661..7044
                     /locus_tag="NBK22_001608"
     CDS             6661..7044
                     /locus_tag="NBK22_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCD2414797.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQDTEH"
     gene            complement(7123..7389)
                     /locus_tag="NBK22_001609"
     CDS             complement(7123..7389)
                     /locus_tag="NBK22_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCD2414798.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            7487..7921
                     /locus_tag="NBK22_001610"
     CDS             7487..7921
                     /locus_tag="NBK22_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCD2414799.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVGIRKQFTYDYTALD
                     IKIIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            7921..8700
                     /locus_tag="NBK22_001611"
     CDS             7921..8700
                     /locus_tag="NBK22_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCD2414800.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK"
     gene            8733..9692
                     /locus_tag="NBK22_001612"
     CDS             8733..9692
                     /locus_tag="NBK22_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCD2414801.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLATARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
     gene            9946..10056
                     /locus_tag="NBK22_001613"
     CDS             9946..10056
                     /locus_tag="NBK22_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414802.1"
                     /translation="MITTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
     gene            10104..10256
                     /locus_tag="NBK22_001614"
     CDS             10104..10256
                     /locus_tag="NBK22_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCD2414803.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            10272..11729
                     /gene="dltA"
                     /locus_tag="NBK22_001615"
     CDS             10272..11729
                     /gene="dltA"
                     /locus_tag="NBK22_001615"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCD2414804.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSRKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLEGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYGSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGARLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPKTEVTDNAEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            11726..12940
                     /gene="dltB"
                     /locus_tag="NBK22_001616"
     CDS             11726..12940
                     /gene="dltB"
                     /locus_tag="NBK22_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCD2414805.1"
                     /translation="MIPYGDFTFFLIALIALLPVIVLGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
BASE COUNT         4635 a         1870 c         2407 g         4063 t
ORIGIN      
        1 atcatcatta agactattat atataatgaa ttttaactgg tttattaaac gagaacgtcg
       61 ggaattaagt aactacaata aaaataagat atgacaataa ggagactaca cgcgtgatca
      121 ttgccataat tatattgata tttatttcgt ttttcttttc aggaagtgag acggcattaa
      181 cggctgccaa taaaacaaaa tttaaaactg aagctgacaa aggtgataaa aaagcaaaag
      241 gcattgtaaa gttacttgaa aaaccaagtg agtttattac aacgattcta atagggaata
      301 atgtcgcgaa tattttatta ccaacacttg ttacaattat ggctttacgt tgggggatta
      361 gcgttggtat tgcatcagct gttttaacag ttgttatcat tttgatttcc gaagtgattc
      421 ccaagtctgt cgctgcaaca tttccagata aaataacaag gcttgtatat ccaattatta
      481 atatttgtgt cattgtgttc cgtcctatca cattactttt aaataagttg acggacagta
      541 ttaatcgaag tttatctaag ggccaacctc aagaacatca attttcaaaa gaagaattta
      601 aaacaatgtt agcaattgct ggacatgaag gtgctttaaa tgaaattgag acgagtaggt
      661 tggaaggtgt cattaatttt gaaaatttaa aagtaaaaga tgtagataca actcctagaa
      721 ttaatgtgac ggcatttgct tcaaatgcga catacgaaga agtttatgaa acggttatga
      781 ataagccata cactagatat ccagtgtacg agggagatat tgataacatt attggagtgt
      841 ttcattctaa atatctgttg gcttggagta ataaaaaaga aaatcaaatt acaaactatt
      901 cagctaagcc attatttgtg aatgaacaca ataaagctga atgggtatta cgtaagatga
      961 ctatttctag aaaacattta gcaattgtgt tggacgaatt tggtggtact gaagcgatag
     1021 tgtcacatga agacttaatt gaagaattat taggtatgga aattgaagat gagatggata
     1081 aaaaggaaaa agaaaaactt tctcaacagc aaattcaatt tcaacaacga aaaaatcgca
     1141 acgtatctat ataaggagcg aacagctatg tggaataaga atcgacttac tcaaatgtta
     1201 agtattgaat atccaattat acaagcaggt atggcaggaa gtacgacacc gaaattagtt
     1261 gcatcagtaa gtaacagtgg tgggttaggc acaataggcg caggttactt taatacgcag
     1321 caattggaag atgaaataga ttatgtacgc caattaacgt caaattcttt tggcgtaaat
     1381 gtctttgtac caagtcaaca atcatatact agtagtcaaa ttgaaaatat gaatgcatgg
     1441 ttaaaacctt atcgacgcgc attacattta gaagagccgg ttgtaaaaat taccgaagaa
     1501 caacaattta agtgtcatat tgatacgata attaaaaagc aagtgcctgt atgttgtttt
     1561 acttttggaa ttccaagcga atctattata aaaagattga aagaagcaaa cattaagcta
     1621 ataggtacag caacaagtgt tgatgaagct attgcgaatg aaaaagcggg tatggatgct
     1681 atcgttgctc aaggtagtga agcaggtgga catcgtggtt catttttaaa acctaaaaat
     1741 caattaccta tggttggaac aatatcttta gtgccacaaa ttgtagatgt cgtttcaatt
     1801 ccggtcattg ccgctggtgg aattatggac ggtagaggag ttttggcaag tattgtctta
     1861 ggtgcagaag gggtacaaat gggcactgca tttttaacat cacaagatag taatgcatca
     1921 gaactactac gagacgcaat tataaatagt aaagaaacag atacagtcgt tacaaaagcg
     1981 tttagtggaa agcttgcacg cggtatcaac aataggttta tcggagaaat gtcccaatac
     2041 gaaggcgaca tcccagatta tccaatacaa aatgagctaa caagtagcat aagaaaagcc
     2101 gcggcaaaca tcggcgacaa agagttaaca catatgtgga gtggacaaag cccgcgacta
     2161 gcaacaacgc atcccgccaa caccatcatg tccaatataa tcaatcaaat taatcaaatc
     2221 atgcaatata aataatcgaa tcgaccgcaa tccacaaaag cacaagcacc cccaaacatt
     2281 attttagtgc ttgccatttt tgtggattgc gtttctattt taccaatcta atcaaacgaa
     2341 attatcgagc tgaaggtcac cgaaatgatt ataatcaaac gaaaacatca agctgaagat
     2401 cgccgaaaga ttatgatcaa gcaaaaacat caaattaaag ttcgctgaaa tgattatgat
     2461 aaaagttata tggtatgatg acattggtga tatatatgat aaacatcgga ttgacaggtt
     2521 ggggtgatca ctattcatta tatgaagatt tagaacgcca aaccgataaa cttaaaacat
     2581 atgctggaca ttttccggtt gtcgaattag atgcgacata ctatgcgata caaccggaaa
     2641 gaaatatatt gaaatggata aaagaaacgc ctgatacatt tgaatttgtg gtcaaaattc
     2701 atcaagcact cacattgcat gcagactaca aaacatttgc agatacaagg caagaactat
     2761 ttgatcaatt taagaatatg ttagagccct tacatacaca gaaaaaatta gcaatggtat
     2821 tggttcaatt tccgccatgg tttgactgca atgcacaaaa tatcaaatat attttgtatg
     2881 taagacagca attacaagca tttccaatat gtgtagaatt taggcatcaa tcatggttta
     2941 gtgatgcatt taaagaacaa acattggcat ttttaacaga acatcaaatc attcatgcag
     3001 tagttgatga accacaagtg aaagatggca gtgtaccttt agtcaatcga atcacaaatg
     3061 aaattgcgtt tgtacgttat catggacgta atcattacgg ttggactaag aaagatatgt
     3121 cagatcaaga atggcgagat gtacgctatt tatatgatta taatgagcaa gaattaatag
     3181 acttggcaca aaaggcacaa atattagcac aaaaagctaa gaaagtttac gtcatattta
     3241 acaataattc tggtggtcat gcagcaaata atgccaaaac atatcagcga ttattgaata
     3301 tagaatatga agggttagca ccacaacaat taaaattatt ttaagaggcg acgactatgt
     3361 tattaacaat tacattatta gttttaatcg gaggtttgtc agcgattata gggtctatcg
     3421 taggcattgg aggcggtatt attatcgttc caacaatggt ttacctcggt gttgaacatg
     3481 gattactaca taatattaca acacaagtag cgatagggac gtcttcagtc attctaattg
     3541 tgacaggact ttcttcatca cttggatatt taaaaacaaa acaagttgat attaaaaatg
     3601 gttccatctt tttatttgga ctattaccag gttcattgct tgggtccttc attagtagat
     3661 atttaacatt tgagtcattt aatttatatt ttggtatctt tttaattttc gtagccattt
     3721 tattaatggt aagaaataag attaaaccgt ttaaaatttt tgataaaccc aagtatgaaa
     3781 agacttatgt agatgctaaa ggtaaaacat atcattatag tgtgccacca ttgtttgctt
     3841 ttattacaac gtttttaatt ggtatattga caggtttatt tggtattgga ggtggcgcac
     3901 taatgacgcc actaatgctt attgtattta gatttccacc tcatgtagct gttggaacaa
     3961 gtatgatgat gattttcttt tcaagtgtca tgagttctat agggcacatt gctcaaggtc
     4021 acgtagcttg ggggtatgca atcattttaa ttatttctag ttattttggt gcgaaaatcg
     4081 gtgtcaaagt gaatcaatca attaagtcag atacggtagt aacattattg agaacagtaa
     4141 tgttgttaat gggtatatat ttaattattc gtgcgttgat ttaatacaac tttaaaagga
     4201 ggacgtcaat ttgaggctta caatttatca tacgaacgat attcatagtc atttacatga
     4261 atacgaacgc attaaagcat atatggcaga acatcggcca cgacttaacc atccttcttt
     4321 atatgttgat ctaggtgatc atgtagattt atccgcaccc ataactgaag caactttagg
     4381 taaaaagaat gtggcattac taaatgaagc aaaatgtgat gttgcaacaa tcggtaataa
     4441 tgaagggatg accatttcac acgaagcttt aaatcacctt tacgacgaag caaaatttat
     4501 agtgacatgt agcaatgtta tagatgaatc aggtcattta ccaaataata tcgtttcttc
     4561 ttatattaag gacatagacg gtgtgaaaat actattcgtt gcagcgacag caccttttac
     4621 cccattttat cgtgcactaa attggattgt taccgatcca cttgaatcta taaaagaaga
     4681 aattgaactt caacgaggta aatttgacgt attaatcgtg ctaagtcatt gtggcatttt
     4741 cttcgatgaa acattatgcc aagaattgcc tgaaattgat gtcatttttg gtagtcatac
     4801 gcatcattat tttgaacatg gtgaaatcaa taatggtgta ctgatggcgg cagctggaaa
     4861 gtatggtaat tatcttggag aggttaattt aacttttgag gcacataaag tagtacataa
     4921 aactgcaaag attattcctt tagaaacatt acctgaagtt gaaacttcat ttgaagaaga
     4981 aggaaaaacg ttaatgtcca attcagtaat tcaacatcca gtagtgctta agcgtagtat
     5041 gaatcacata actgaagctg catacttatt agctcaaagt gtttgtgagt atacacatgc
     5101 acaatgtgcc atcatcaatg ctggcttact cgttaaagat attgtaaaag atgaagtgac
     5161 agaatatgac attcatcaaa tgttaccgca tccgattaat atggtaaggg ttagactttt
     5221 tggtgtgaaa ttaaaagaga ttatagctaa aagtaataaa caagaatata tgtatgaaca
     5281 tgcacaaggt ttgggtttca gagggaatat atttggagga tatattcttt ataatttagg
     5341 gtacattcat tctacagggc gttactatct gaatggagaa gaaatcgaag acgacaaaga
     5401 atatgtacta ggtacgatag atatgtatac gttcggtcgt tatttcccaa cattgaaaga
     5461 attaccaaaa gagtatttaa tgccagagtt tttaagagat atatttaaag aaaaattatt
     5521 ggaatattaa aaagtaagat tattggattt tcatttgtca tgaatttcga tataatgttt
     5581 aaagatacac ttaacaggag ggtatgtgtt gttatggcga caaaaaacga ggaaatatta
     5641 cgtaaaccgg attggttgaa aataaaatta aataccaacg aaaactatac aggacttaag
     5701 aagatgatga gggaaaaaaa tcttaatact gtatgtgaag aagctaaatg tcctaatata
     5761 catgaatgtt ggggtgcacg tcgtacagcg acatttatga ttttaggtgc cgtatgtaca
     5821 agagcttgtc gtttttgtgc ggttaagaca ggtttaccta atgaacttga tttaaatgag
     5881 cctgaacgtg tagctgaatc agttgaatta atgaatttga aacacgttgt tatcactgct
     5941 gttgcgcgtg atgatttaag agatgctggt tcaaatgttt atgctgagac agtacgtaaa
     6001 gttagagaaa gaaatccatt tacaacgatt gaaattttac catcagatat gggcggggac
     6061 tatgatgcgt tagaaacatt aatggcgtca agacctgaca ttttaaacca taatattgaa
     6121 actgttcgtc gcttaacacc gagagttcgt gcgcgtgcga cttacgacag aacattagag
     6181 tttttacgtc gttcaaaaga attacaaccg gatatcccaa ctaaatcaag tattatggtt
     6241 ggattaggtg aaactataga agaaatttat gaaacgatgg atgatttacg tgcgaatgat
     6301 gtagatattt taacgattgg tcaatattta caaccttcac gtaaacattt aaaggttcaa
     6361 aaatattaca cgcctttaga gtttggtaaa ttaagaaaag tggcaatgga taaagggttt
     6421 aaacattgcc aagctggacc tttagtacgt agttcttatc atgcggatga gcaagtaaat
     6481 gaagctgcta aagaaaagca acgccaaggt gaggcacagt taaatagtta atatttaact
     6541 attaataagg cataaaggct tagtttgtac aaaacgaacg tgtcatagaa gtaatcatta
     6601 tttttatgaa acactaaaag tataaactga gcctttcatt gtcatagata ggtgaagaat
     6661 ttgataaaag tagatcaaca ttactttgaa ttaatagaaa attatcgcga atgttttaat
     6721 gaagaacaat ttattgctag gtattcagat attttagata aatatgatta catagttggt
     6781 gactatggtt acgatcaatt acgattaaaa ggtttttaca aagattctaa taaaaaagca
     6841 gagatgagta aacgtttttc aaatattcaa gattacatat ttgaatattg taactttggt
     6901 tgtccttact ttgtattaag acatttgtct aaacaagagg ttaaaaagtt aatcgaagaa
     6961 gttcatccgt ctgatgtgat agatgacgac aataaacttc aagatgtgaa gattaagcca
     7021 accattcaag atactgaaca ttaataaaac ccttagctag attgaaaatg ggaatcatgc
     7081 aattcaagca tggacctgta atctagttag gggtttttat ctttaatgaa tgacttcatt
     7141 taaatactca gtaatttcat cgccttcttc agcatttaca cctaaaatat gagcgatata
     7201 gccttcttct tttaaatcat cagtaccgat aataccgaat ttatttgttt gcatattaag
     7261 tacgagtgtc ttaccataat gtctatttgt atggactaac atcaaatcat atcgactatg
     7321 ctcgccaaca aaaccaacaa actgaacttg actctcttcg ttgtcatcat ataaatacat
     7381 atcaatcatt ttgtagcgac tccttttaaa agtagtaaag ttagtataac gacaaatgaa
     7441 gtatactgca aaattatgat aatatataag tgagaggtga caaggaatgt attttgtaga
     7501 caaagataaa ctaactcaga aattagccta tttacaagca ttaactgatg attatcatga
     7561 gagcaagcac aatcattatg catttgaacg cattgctcaa atgttgatag aatcatcggt
     7621 agatataggg aatatgatta tcgatgcatt tattttaagg gatcctggta attataaaga
     7681 tgtgattgat atattagaac tagaaaatgt tattactaaa gaaacacagc aggcgattaa
     7741 taaaactgtc ggtattcgta aacaatttac atatgattac acagccttag atattaagat
     7801 tatcatgcca atgtttgatg acgcattacc ttattacaaa caatttatta cagaagtaac
     7861 gacattttta catcaagaaa atgtaccagt aacagctttt ggtaaaggag aaaatcaata
     7921 atgaaacagt ataaagcgta tttaatcgat ttagatggca caatgtatat gggaacagat
     7981 gagattgatg gagcaaaaca attcatcgat tatttaaatg taaaaggcat tcctcattta
     8041 tacgtaacta ataattcaac aaaaacacct gagcaagtaa ctgaaaaatt acgtgaaatg
     8101 cacattgatg ctaaaccaga agaggttgta acgtcagcgt tagccactgc tgattatatt
     8161 tcagaacaat caccaggagc atcagtatat atgttaggtg ggagtggttt aaatactgcg
     8221 ttaaccgaag cgggacttgt cattaaaaat gacgagcatg ttgattatgt agttattgga
     8281 cttgacgaac aagttacata tgaaaagctt gcgattgcaa cgttaggtgt aagaaatggt
     8341 gcaacattta tttctacaaa tcctgatgta tcaattccta aagagcgtgg tttattacct
     8401 ggtaatggtg ctattacaag tgttgtaagt gtatcgacag gtgtatcgcc acaatttatt
     8461 ggtaaaccag aaccgattat tatggttaaa gcattagaaa ttttaggatt agataaatcc
     8521 gaagttgcta tggtaggcga tttgtacgat accgatatta tgtctggtat taacgtaggt
     8581 atggatacga ttcatgtaca aacaggcgta tctacgttag aagatgtgca aaataaaaat
     8641 gtgccaccaa cgtattcttt taaagattta aatgaagcaa tagctgaatt agaaaaatag
     8701 atatagtcat tttataaagt aggtgaattg atttggtaaa aatagttgtt tcgaggaaaa
     8761 ttccagataa attttatcaa caattaagta aacttggtga cgttgttatg tggcaaaaat
     8821 cattagtgcc tatgcctaaa gatcaatttg tgacagcact tcgtgacgca gatgcttgtt
     8881 ttatcacttt aagtgaacag atcgatgcag aaattttagc acaatcacca aatttaaaag
     8941 taattgcgaa tatggctgta ggatatgaca acatcgatgt tgaaagtgca acagcgaata
     9001 acgtggttgt cacgaataca ccaaatgtac ttactgaaac aactgcagaa ttaggattta
     9061 cattaatgct tgctacagca cgccgtattg tagaagctga aaaatatgta gaggcagatg
     9121 catggcaaag ctggggtcct tatttattgt caggtaaaga tgtcttcaat tcaactattg
     9181 gaatatatgg tatgggggat attggtaaag cttttgcaag aaggttgcaa gggtttaata
     9241 ctaatattct ttatcataat cgatcaagac ataaagatgc agaggcggac tttaatgcaa
     9301 catatgtttc ttttgaaacg ttgttagcag aaagtgattt tatcatctgt acagcgccac
     9361 ttacaaaaga aacacatcat aaatttaatg ctgaagcatt tgaacaaatg aaaaatgatg
     9421 caatttttat taatatcggt agaggacaaa ttgtagatga aacagcatta attgatgcac
     9481 tagacaataa agaaatttta gcatgtggtt tagatgtatt agcaaatgaa ccgattgatc
     9541 atacacatcc attaatggga cgtgataatg ttctgattac accacacatt ggtagcgcat
     9601 cagtaacaac acgggacaat atgattcaat tatgtattaa taatatagaa gcggttatga
     9661 caaatcaggt accacatact ccagtaaatt gaaaataagt atggttttta atcattggta
     9721 aaacaagcaa agcaattgtt aaatatgtta tgcttgactt gcgaatgtat atatttgcgc
     9781 tgatgataat tcaataacat tataaactcg tcatattaat gtgatgagtt tatttgattt
     9841 tgacgtatat aacaaaattg ttatatagac tgtaattgtt tattgtttaa ataaccttta
     9901 aaatgaatga tgttctcaac aaagtgtatc atttacaata attgagtgat cacaaccaat
     9961 tactatgttg aatctataaa attaaaattg aatttcatta tgaatatcga tataatgaat
    10021 tgtaaaaaac aaattttaaa acgtatttta tattgaacgg tctttataaa ggcgttttta
    10081 ctaagaactt taagaggtgc aatatgaaat ctaaaagtaa acagccacct aataaatatg
    10141 ttgaagcatt caaaccatat ttattaacac tattgtattt ggcaatattt attactttat
    10201 atttaattta tggcagtggc gacacacaca ataacttcat ttataatgag ttctaatgag
    10261 ggagacttaa tatgacagat attattaaca agctgcaagc gtttgcggat gcaaatccac
    10321 aaagcattgc tgttagacac acaactgatg aattaactta tcaacagtta atggatgagt
    10381 ctagtaaatt agcacatcga ttacaaggta gtaggaaacc gatgatttta ttcggtcaca
    10441 tgtcaccata tatgattgtt gggatgattg gtgccattaa agcaggatgt ggatatgtac
    10501 ctgtagacac ttcaattcct gaagaccgta ttaaaatgat tattaacaag gttcaaccag
    10561 agtttgtatt taatacgact gatgaatcat ttgaaagttt agaaggcgaa gtatttacaa
    10621 tagaagatat taaaacatct caagacccag taatttttga tagtcagatt aaagataacg
    10681 acacagtata cacaatcttt acatctggtt ctactgggga acctaaaggt gttcaaattg
    10741 aatatgcaag tttagttcaa tttactgagt ggatgttaga acttaataaa tcaggaaatg
    10801 aacaacaatg gcttaaccaa gcgccatttt catttgattt atctgtaatg gctatttatc
    10861 catgtttagc atcaggcggt acattaaatc ttgtagataa aaacatgatt aataaaccta
    10921 aattattaaa tgaaatgcta acagcaacac cgattaacat ttgggtatca acaccatcat
    10981 ttatggaaat gtgtttatta ttaccaacac ttaatgaaga acagtatggt agtcttaacg
    11041 aattcttctt ctgtggtgaa attctacctc acagagcagc aaaagcgtta gtaagccgtt
    11101 tcccaagtgc gacgatttac aacacatatg gtccaactga agctacggta gcagttacaa
    11161 gtattcaaat tacacaagaa atcttagatc aatatccgac attacctgtt ggtgttgaaa
    11221 gaccaggcgc aagattatct actacagatg aaggtgaact tgttatcgaa ggtcaaagtg
    11281 taagtttagg atacttaaaa aatgaccaaa aaacagctga agtatttaat ttcgatgacg
    11341 gtattcgtac atatcacact ggtgataaag cgaagtttga aaatggtcaa tggttcattc
    11401 aaggtcgtat tgatttccaa atcaaattga atggctacag aatggaatta gaagaaattg
    11461 aaacacaatt acgccagtct gagttcgtaa aagaagcgat tgttgtacct gtatataaaa
    11521 atgataaagt tattcattta attggtgcaa ttgtgccaaa gactgaagtt acggacaatg
    11581 cagaaatgac taaaaatatt aaaaatgact tgaaatcacg cttaccagag tatatgattc
    11641 ctagaaagtt tgaatggatg gaacaattgc cattgacttc aaatggtaaa attgacagaa
    11701 agaaaattgc agaggtaatt aacggatgat tccatatggt gattttacat tcttcttaat
    11761 tgctttaatt gcattattac cagtcattgt acttggattt ttaggtaagc gaagttacat
    11821 ttataatggc gtagttacag catttatgat tgtgttaatc ttttcttctg ataaacataa
    11881 tctgtttgac caaaagtatt taagtgttca attaattagt tttattattt acgtcgtatg
    11941 gcaagtttta ttgataatgt tttattatca ttcaaaacca aaaaataatt cattttcaaa
    12001 atttgtaact gtaatggttt tatcaatatt gccattagca cttgtgaaag tgttacaaag
    12061 tacatggtta ggtggacatc agattcactt ccatgaaagt aaattaattg aatttgttgg
    12121 tttcttagga atttcttatg ttacattcaa aagtgtgcag ttaattatgg aaattcgtga
    12181 tggttctatc aaagaaatta aagtatggaa attaattcaa tttatttcat tcttcccaac
    12241 gatttcatct ggaccaatcg atcgttacaa acgtttcgtt aaagacgata aaaaagtacc
    12301 aacaggcaat gaatatcgcg aattagtatt aaaagcaatt cacatgatta tgcttggttt
    12361 cttgtataaa tatattgttg cttactttat taacacatat gcaatcatgc cgttacaatt
    12421 agacttacat ggctttgtca atttgtggtt atatatgtac gcatacagct tatatttatt
    12481 ctttgacttt gcaggttata gtttatttgc gatagcattt agttatttat tcggtattaa
    12541 aacaccacca aacttcgata aacctttcaa agcgaaaaat attaaagatt tctggaatag
    12601 atggcatatg acattatcat tctggttcag agattgtatt tacatgagat ctttattcta
    12661 catgtctcgt aaaaaattat tgaagagtca atttgcaatg tctaacgtgg cattcttaat
    12721 caacttcttc ataatgggaa tttggcatgg tatcgaagtg tattacattg tttatggttt
    12781 ataccatgca gcattgttta taggttatgg ctattatgaa cgttggcgta agaaacatcc
    12841 gccacgttgg caaaatggtt tcacaacagc acttagcatt gtgattacat tccactttgt
    12901 aacatttggc tttttaatct tctcaggtaa acttatataa taaaggagaa tttaattatg
    12961 gaatttagag aacaa
//
LOCUS       DAHHYJ010000076        12295 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.076, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000076 DAHHYJ010000000
VERSION     DAHHYJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.076"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..103
                     /locus_tag="NBK22_001617"
     CDS             <1..103
                     /locus_tag="NBK22_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="poly(glycerol-phosphate)
                     alpha-glucosyltransferase"
                     /protein_id="HCD2414806.1"
                     /translation="VLAEEFGSKARENIIEKYSTESILEKWLNLFNS"
     gene            complement(192..548)
                     /locus_tag="NBK22_001618"
     CDS             complement(192..548)
                     /locus_tag="NBK22_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD2414807.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            1036..1995
                     /locus_tag="NBK22_001619"
     CDS             1036..1995
                     /locus_tag="NBK22_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2414808.1"
                     /translation="MNRNIVKLVVFMLILVVAVAGCGQKDTEEKTEMTTIKDELGTEK
                     IKKNPKRVVVLEYSFADYLAALDMKPVGIADDGSTKNITKSVRDKIGAYESVGSRPQP
                     NMEVISKLKPDLIIADVSRHKKIKSELSKIAPTIMLVSGTGDYNANIEAFKTVAKAVG
                     KEKEGEKRLEKHDKILAEIRKKIEQSTLKSAFAFGISRAGMFINNEDTFMGQFLIKMG
                     IQPEVTKDKTTHVGERKGGPYIYLNNEELANINPKVMILATDGKTDKNRTKFIDPAVW
                     KSLKAVKDNKVYDVDRNKWLKSRGIIASESMAEDLEKIVEKAK"
     gene            complement(2041..2172)
                     /locus_tag="NBK22_001620"
     CDS             complement(2041..2172)
                     /locus_tag="NBK22_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414809.1"
                     /translation="MRYVTMRQFIKRIIKTILVGYVIKFIRNKLSGKSSHPTDNKHN"
     gene            complement(2236..2451)
                     /locus_tag="NBK22_001621"
     CDS             complement(2236..2451)
                     /locus_tag="NBK22_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCD2414810.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADKDGYIIFPKN"
     gene            2616..3167
                     /locus_tag="NBK22_001622"
     CDS             2616..3167
                     /locus_tag="NBK22_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2414811.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWELLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYK
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(3219..4157)
                     /locus_tag="NBK22_001623"
     CDS             complement(3219..4157)
                     /locus_tag="NBK22_001623"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCD2414812.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRAKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            4339..5700
                     /locus_tag="NBK22_001624"
     CDS             4339..5700
                     /locus_tag="NBK22_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCD2414813.1"
                     /translation="MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KVISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDNLHPTPALGGYPKDFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            5687..7360
                     /gene="menD"
                     /locus_tag="NBK22_001625"
     CDS             5687..7360
                     /gene="menD"
                     /locus_tag="NBK22_001625"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCD2414814.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLKVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTVNRVSWLEKWQRLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDIYANRGANGIDGIVSTALGMAVHKRVTLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            7347..8150
                     /gene="menH"
                     /locus_tag="NBK22_001626"
     CDS             7347..8150
                     /gene="menH"
                     /locus_tag="NBK22_001626"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCD2414815.1"
                     /translation="MTHYKYYEANVETNQVLVLLHGFLSDSRTYYNHIDKYTDICHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEK
                     FVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            8143..8964
                     /gene="menB"
                     /locus_tag="NBK22_001627"
     CDS             8143..8964
                     /gene="menB"
                     /locus_tag="NBK22_001627"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCD2414816.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(9202..9531)
                     /gene="sspC"
                     /locus_tag="NBK22_001628"
     CDS             complement(9202..9531)
                     /gene="sspC"
                     /locus_tag="NBK22_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCD2414817.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(9569..10750)
                     /gene="sspB"
                     /locus_tag="NBK22_001629"
     CDS             complement(9569..10750)
                     /gene="sspB"
                     /locus_tag="NBK22_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCD2414818.1"
                     /translation="MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLSLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DQKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSSKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(10832..11860)
                     /gene="sspA"
                     /locus_tag="NBK22_001630"
     CDS             complement(10832..11860)
                     /gene="sspA"
                     /locus_tag="NBK22_001630"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCD2414819.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQS
                     SKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDNN
                     NSDNPDAA"
BASE COUNT         4332 a         1725 c         2224 g         4014 t
ORIGIN      
        1 tgttttagca gaggagtttg gttcgaaagc gagagaaaac attatagaaa aatattcaac
       61 ggaatcaata ttagaaaaat ggttaaatct tttcaatagc taaatgaaaa aatcctaggt
      121 attcatgagt acctaggatt tttacgcatg atatatacaa aaaaagagag gatagtcctc
      181 tcaaataata gttaataaaa ttgtagccat ttcttccagc tgtaactact aatacaagct
      241 aagttcatta cgatatgtat gatttcaaat tcataaccac ttacatatcc tcttgtaagt
      301 ttaagtgtga agattgagcc taacataaca caaatataaa aaacattaac ataaggcgtt
      361 aaaaagccga taataagtgc taaacctcca acaacttcac caattgacat caatactgcg
      421 atggtaggtg gtaaacctaa agagcctaag aagtgaattg ttccatctaa atcaacaaat
      481 ttcaaagtac catgcactaa gaatgatgtt gctaacatcc atctaattaa aagcatacct
      541 aatttcatca tgtcacctcc aacattcata tttttaaaac atagtccaaa ttatatttgt
      601 aaacatgtgg aaagtaaaga ttttttataa gattaattat taaatattta ttaaatattc
      661 tattgtattg aacaattgtt gtagaaagat gtttaagtca tcagttactt tttgtattta
      721 taaataaaga ttagtacagc atagtgcaaa gattataggg aaatgaatta actattgcat
      781 cgctatttat gaatcgtaaa cactattatg aaattgcgag agtagtatgg ataattagaa
      841 tgatggtttg agataaatga gtattattaa ccagtgcgtc atcttcaaaa ttagaggcca
      901 tttgctgttt ataataagaa gatttgataa taaactatga taagaaaaaa taatagcata
      961 ccaaatatat cataaacaaa ttaaatgata aaataataat gtagttttct attaaagttg
     1021 tttggaggta aatatgtgaa taggaatatc gttaaattag ttgtgtttat gctaatctta
     1081 gttgtagcag tagcgggttg tggtcaaaaa gatactgaag agaaaactga aatgacgaca
     1141 ataaaagatg aattaggaac tgaaaaaatt aagaaaaatc ctaaacgtgt tgttgtatta
     1201 gaatatagtt ttgctgatta tttagcagca ttagatatga aacctgttgg tattgcagat
     1261 gatggcagca ctaaaaatat aacaaagtca gtaagagata agattggggc atatgaatcg
     1321 gttggatcta gaccgcaacc gaatatggaa gtgataagta aattaaaacc ggatttgatc
     1381 attgcagatg ttagcagaca taagaaaatc aaatcagaat tgagcaaaat tgctccgaca
     1441 atcatgttag ttagcggtac gggagattat aatgcaaata ttgaagcatt taaaacagtc
     1501 gctaaagcag taggcaaaga gaaagaaggc gagaagcgtc tggaaaagca tgataaaata
     1561 ttagcggaga ttagaaagaa aattgaacag agtacgttaa aatctgcatt tgcattcggt
     1621 atctcaagag caggtatgtt tattaataat gaagatacat ttatgggaca attcttaatt
     1681 aaaatgggta ttcaacctga agtcacaaaa gacaaaacta ctcatgttgg tgaacgcaag
     1741 ggtggtcctt atatttattt aaataatgaa gaacttgcca atatcaatcc aaaagttatg
     1801 attttagcca ctgacggaaa aacggacaaa aatagaacga aattcattga tcctgcagtt
     1861 tggaaatcat taaaagctgt gaaagataac aaagtttatg acgttgaccg aaataagtgg
     1921 ttgaaatcaa gggggattat cgcaagtgaa agtatggcag aagatttaga aaaaattgta
     1981 gaaaaagcaa aataaaaata cagcgccact cgtaaatcat ataagagtgg cgctgtattt
     2041 ttaattatgt ttattatctg tcggatgtga tgatttacct gaaagtttat ttcgaataaa
     2101 tttaattaca taaccgacaa ggattgtttt aataattctt ttaatgaatt ggcgcatcgt
     2161 tacatacctc atttctctat atcttacgaa ctatataccc attcatatat gctttttaaa
     2221 cgtcattgtc acaatttaat ttttagggaa tataatataa ccatctttat ctgctttttt
     2281 agtaaaaatg acaaaaatgg catgtattat tgagatgatg gtagggatac ctgtccagaa
     2341 aaataataag tgaaaaagac cttgtccaaa tttatcagca taaaatttat gaatacctaa
     2401 acctccaaga aataatgcaa caataacata aatggcttta ttgactttca tttgtaaatc
     2461 ctccttaact ataattctat ttaaattcgt tgtgaaaacc aatatttcta actttagaat
     2521 tttcaaactt tctaaaatta taagtatatc tttttaaaat aagctagaat ttctatataa
     2581 taaatgttaa taacgtaaaa gggaatgatg acatagtgat acgtcaagca cgtccagagg
     2641 accgatttga tattgcgaag ttagtttata tggtttggga tgatatggaa ttagaattgg
     2701 taaagcatct acctaaagac atggtattag atgcaattga aaaaagctgt gttgatgcaa
     2761 catatcgaac tttttatcag catattttag tttatgaagt agaaaataaa gtagcaggtt
     2821 gtattattag ctatagtggt gaaaatgaat tgaaatacga aaaagcatgg gaactacttg
     2881 acttgccaga agaaataaaa caatatggca cgccattacc tgtaaaagaa gctaaagacg
     2941 atgagtatta tatagaaaca attgcgacat ttgcagcata tagaggtaga ggcatcgcga
     3001 caaagttatt aacgtcatta cttgaatcaa atacacatgt taaatggagt ttgaattgcg
     3061 atattaataa tgaagcagca ttaaagttat ataaaaaagt aggctttata tctgatggac
     3121 agattgaatt atacaagcac atgtatcatc atttaattgt taaataaaaa aactcgacag
     3181 ttcgatgtaa gtcgattgcc gagtagtata attttatatt aaatgcctgc aaataatgca
     3241 ctaatataaa tacctaatgc atataataaa ccgaaaaatg tatttgtttt accagcagca
     3301 gccattgctg gcatcattgt aggcggtgta tcattcttct tgaaacgtct gataacttta
     3361 acgggcattg ggaatgataa caacgcaagt aagtaaaata atgagccacc aggtttaata
     3421 atgatcgtaa gtacaataaa tgcgtaagcg ataaagtaca tgattgccat aaatgttaaa
     3481 gaagcatttt tacctaatag aatgggtaaa gttttacgac cacttgcttt atctttggca
     3541 cggtcgcgaa tattgttagc catattaatt aaaccgatag tgattactat aggtacactt
     3601 aaccaaatta cataactttg aatattgcca gtttgaataa agaatgcaat aacgataata
     3661 aacataccca taaatacgcc tgagaataat tcaccgaaag gcgtccatga aatagggaaa
     3721 gggccacctg tatataggta accaacagcc atacatacta atccaactgg taataaccaa
     3781 aatgaagagt tagcagctaa aaacaaacct aatattgctg ctaagatgta aaatgcaatg
     3841 gctaatcgta gcacaagctc tgggctcata ccgttgcgaa caatggcacc accaatgcct
     3901 acagattcat gatcatcaag gcctttttta taatcatagt attcattaaa catattagtt
     3961 gctgcttgaa taagtaagca tgctagtaac atggcaatga ataggctgat tttaatatga
     4021 tcttcgctac caagaaaata tattttagat gctgctgtac caactaaaac gggtactacg
     4081 gaagcagtta atgtatgagg acgcattaaa tgccaatatt tcttaactgt agaatattgc
     4141 tgatattgat tactcattga atacaaaacc tctttaaatc atatttttta taacaaagat
     4201 attttaatag aaagtagcat aaaggtcaat tgacgtgaaa aattgcaaga taagtttaag
     4261 caattcttgt aacacaatta tccaaaaatg atacaatata ataatgagtg aaaaaagacg
     4321 aagaaagaag tgaaatggat ggctacgggc gtattagagg acgatattgt caaagagata
     4381 tatggcagct caaaggaatg ggtttcagtt gaagtgaaat tatcacagtc actagacccg
     4441 agcacattat ttcatctcac tgacaatgag gcaggagatc gcttttatat gcgtttgaat
     4501 gataatcgaa cgtcatattt tggctacaaa gcaattcaat tattcaaaaa taattctaaa
     4561 aataaacaat ctatttttaa agactgggaa aaattaaaac ataacatcac atttatacat
     4621 ccgcaatctg agaaacatca tcttcgagtt gttggagggt tccaattttc aagtcataaa
     4681 tcagatgatg aatggcgaga gtttggacta aatcattttg tattacctga agttttaatt
     4741 tcaactgata ataatgggac atttttaact tatacagtta aaagggaaag ttttactgtt
     4801 gaggcattga acgatttaat ggatttgttc aacaatatat cggacataga tgtggacgag
     4861 caaattgggg aaattactag aaatgaagat atttataaag atgactggcg tcaacttgta
     4921 gtagaagcta tagaatctat taataatgaa gaaaaaattg tactagcacg tagacggtta
     4981 ataaagttcg ataaagttat cagtattcca tatattctaa agcaagcata ttctaaagaa
     5041 aaaaacagtt atatattctt gttagaatca caagattcta tattcttttc acaaacacct
     5101 gaacaattaa taaaggtcaa taataaaata ctatcgacta aagctgtagc aggtacaatt
     5161 aaacgttcac aagatgagga cgaagataca aaaaatgttg aagcattttt aaaagataat
     5221 aaaaacttaa tcgaacatcg atttgttgtt gacagtattt tacatgatat taaaccttat
     5281 atcactgaat tacattatga taagacgcct aaaattctaa aaaatgatca tttatatcac
     5341 ttatacactg aaataaaggc gccactgaag gatgattcgt atattagttt aattgataat
     5401 ttacatccaa cacctgcttt aggtggctat ccaaaagatt ttgcaatgga ttttattgag
     5461 cagaaagaat ttggtacgcg aggattatat ggtgcgccgg ttggctatat agatatatat
     5521 gatgattgtg aatttattgt tgcaattcgt tcgatgctta ttaagaaagc acaagcaact
     5581 ttatttgctg ggtgtggcat tgttaaagat tctgatccag atagtgaatt ggcagaaacg
     5641 aaccttaagt tcacacctat gatgaatgca ttaggagtcg atatgaatgg gaaatcataa
     5701 agcagcttta acgaagcaag tttttacatt tgcatctgag ttatatgcgt acggcgtaag
     5761 ggaagtagtt atcagtccgg gatcacgctc aacgccactt gcacttgcat ttgaagcaca
     5821 tccaaatatt aaaacatgga tacaccccga tgagcgaagt gcagcgtttt ttgcagttgg
     5881 gttaattaaa ggtagtgaaa gacctgtcgc tatattatgt acgtcaggta cagcagcagc
     5941 gaattatacg cctgcaattg ctgaaagcca aattagtaga attccattaa tcgttttaac
     6001 aagtgaccgt ccgcatgaat taagaagtgt aggtgcacca caagcgatta atcaagtaaa
     6061 tatgtttaat aattatgtaa gttatgagtt cgatatgcct attgcggatg atagtaaaga
     6121 gaccattgat gcaatttact atcaaatgca aattgctagt caatatttat atggaccaca
     6181 taaagggcca attcatttta acttgccatt tagagatccg ttaacacctg atttgaatgc
     6241 aacagaattg ttaacttctg agatgaagat tttaccgcac tatcaaaaaa gtatagatgc
     6301 atcggcatta agacacattt taaataagaa aaaaggttta attattgtag gggatatgca
     6361 gcaccaagaa gttgatcaaa tactaacgta ttcaacgata tatgatttgc ctattttagc
     6421 tgatccttta agtcatttaa gaaaatttga tcatccgaat gttatctgta catatgattt
     6481 gttgtttaga agcggtttag acttaaaagt cgatttcgta attcgtgttg ggaaaccagt
     6541 gatttctaaa aagttgaatc aatggttaaa gaaaactgat gcatttcaaa tattagtgca
     6601 aaacaatgat aagattgatg tctttccaat agctccagat atttcatatg aaatatctgc
     6661 gaatgatttc tttaggtcat taatggaaga cacgacagtt aatcgcgtaa gttggttaga
     6721 aaaatggcaa cgcttagaga aaaaagggcg taaagaaatt aaatgttatt tggaacaagc
     6781 tacagatgag agtgcattcg ttggtgaatt gattaagaaa acatctgaaa aagatgcatt
     6841 atttattagt aatagtatgc caatcagaga tgtagataac ttgttattga ataaaaatat
     6901 agacatctat gcaaatcgtg gcgcaaatgg tattgatggt atcgtatcaa ctgcactggg
     6961 tatggctgtc cataaacgag ttacattatt gataggtgat ttgtcatttt atcatgatat
     7021 gaatggacta ttaatgtcaa aattaaataa tattcagatg aatattgtat tattgaacaa
     7081 cgatggtggc ggtatttttt catatttacc acaaaaagaa agtgcaactg actattttga
     7141 acggttgttt ggcacaccga cgggattgga tttcgagtat acagctaagt tatatcaatt
     7201 cgattttaaa cgttttaaca gtgtttcaga atttaaaaat gccacattat tatctgaaac
     7261 ttcgacgatt tatgaattga taacgaatcg cgaagataac tttaaacagc atcaaatttt
     7321 atatcagaaa ttgagtgaaa tgattcatga cacattataa atactatgaa gcaaacgttg
     7381 agaccaatca agttttagta ttactgcatg gttttcttag cgacagtcgt acttattata
     7441 atcacatcga taaatatact gatatctgtc atgtcatcac tatagactta ccaggacatg
     7501 gcgaagatca gtcttcaatg gatgaaacgt ggaattttga ttatattacg acgttgttag
     7561 accgaatttt agataaatat aaagataaat caataacatt gtttggatat tcaatgggtg
     7621 ggcgtgttgc attatattat gcaattaatg gtcacatccc tatatctaat ttgatattag
     7681 aaagtacgtc accaggtatt aaagaagaag caaatcaatt ggaacgtcgt cttgttgatg
     7741 atgcacgtgc taaagtatta gacatagcag gtattgaatt atttgttaat gattgggaaa
     7801 agttgccatt atttcaatcg caactagaat taccagttga aatacaacat caaataagac
     7861 aacaacgatt gtcccaatcg ccacataaaa tggccaaagc attaagagat tatggtacag
     7921 gtcaaatgcc aaacttatgg ccgcgcctga aagaaattaa agtaccaaca ttaatattag
     7981 ctggagaata tgatgaaaaa tttgtacaga ttgcgaaaaa aatggcaaat ttaattccta
     8041 atagtaaatg taaattaatt tctgctacag gtcatacaat tcatgtggaa gatagtgatg
     8101 aatttgatac aatgatatta ggatttttaa aggaggagca aaatgactaa cagacaatgg
     8161 gaaacactta gagaatatga tgaaatcaaa tatgaatttt acgaagggat tgctaaggta
     8221 acaataaatc gccctgaagt acgcaatgcg tttacaccta aaacagttgc tgaaatgatt
     8281 gacgcatttt cacgtgcacg tgatgatcaa aacgtttcag ttatcgtatt aactggtgaa
     8341 ggtgatttag cattctgttc tggtggtgac cagaagaaac gtggacatgg tggttatgta
     8401 ggtgaagacc aaatccctcg cttaaatgta ttagatttac agcgtttaat tcgtattatt
     8461 ccaaaaccgg ttatcgcgat ggtaaaaggt tatgctgtag gtggcggtaa tgtactaaat
     8521 gttgtttgtg acttaacgat tgctgctgat aatgctattt ttggacaaac tggtcctaaa
     8581 gtaggttcat ttgatgcggg ttatggttca ggatatttag cacgtatcgt tggacataag
     8641 aaagcacgtg aaatttggta cttatgtcgt caatacaatg cacaagaagc tttagatatg
     8701 ggtctagtaa atacagtggt acctttagag aaagttgaag atgaaactgt gcaatggtgt
     8761 aaagagatta tgaaacactc accaacagcg ttacgattcc ttaaagcagc tatgaatgct
     8821 gacacagatg gtttagctgg tttacaacaa atggctgggg atgcaacact gctttattac
     8881 acaactgatg aagcgaaaga aggccgtgat gcgtttaaag aaaaacgtga tcctgacttc
     8941 gatcaattcc ctaaattccc ataagttatt ttgaatatga tattaagtca cttgcgtcgt
     9001 ttattagcga cgataggtgg cttttttatt ttataagaat ttaaaagggg cttttaatta
     9061 attatcataa atataagata gtttgagaaa attaatagag aaaaaataaa tgttaaagat
     9121 tattattaca atcaaaaaac gagctggtga gaaattgaat tctcgccaac ccgctttatg
     9181 aagtgaaatc atttaaatta gttatactaa gcgctcataa acgattgggc gcgaagtgcc
     9241 aataccttgt ttggatgacg tttgcatgag gatatgctgt gtatcatcat aatcaagaat
     9301 ataaatgatt tcttcattat caatagatga aaatttaatg cgttgatgtg ccgtatctat
     9361 aaaaagaata tgatattgat tgtgacttgt atcatcgcca tgacgtatac aaattgattt
     9421 ttgtaattga tgattactcc aattaccaat gaataaatta atattggttt gttctagatg
     9481 tgtgagtttg gttgtgtcgt aaactaaatt tataaattgt agttgataca tagttttacc
     9541 tgcctttaat caatatctat attacttttt agtaacctat cattgaacca taccagttat
     9601 aatcacgatt gaatgataaa tgtaataggc ttgaatctgc atcttgaatt gataattctg
     9661 tatcccaagg attccagtaa ataagttttt cttggtcatt aattttagca ttaccaacaa
     9721 ctgctagcgc atgtcctaaa tgtgggtcat tagggttttg agatacactt tgtgcaagga
     9781 tcataattcc tacattatct tttgtaagtt gatcaacttg ttcatatgat ggtacgcctt
     9841 cttgataatg aatatctctg ccttgtgatt taccgtattc aatcatttga ttagggaatg
     9901 ttgcacaatt aggaaggtct tgctcactta cttcagggta taatgtacgc ataatatcat
     9961 gtgcattata agtgtctgta tttttagttg catttaataa tgcagccata ctgaatcctg
    10021 cacaccatga gttatcgaat tgttgttctc taattttgaa gttttttaat gtattttcat
    10081 attgaacttg atcttcttgt atcgcttcgt tttcttcaac tttagtaggt gttactgtat
    10141 tttttaactc ttgtttcaat tttgatgaaa ctgttttagc actttctttt tctttcacat
    10201 taccaggtag tggcgtagct tttactaatc taactttgcc atcttcttca aaataaaacc
    10261 ctttttgatc agtaagaaca gtgatatttg aatttttatc tttaatttgg tctaaatctt
    10321 tagcgatgaa gtttgaaatt ttaacactgt aattcatgtc ttcttttgat ttgtttaaat
    10381 catctttatt tttagggcta agtgataaag tataaacaat tttaccgtct tttataactg
    10441 gataatagta actattatct tcttcaccat taaatttata aattttaaaa gcttcgccaa
    10501 gttcatattt accagttttt gcattacttt gtttatctaa tgctttagca taaccagcaa
    10561 attgttgttg tgctagatct tttacttttt gaggtacttt gtcactctta acattaattt
    10621 ctagctgttt agagtgtgaa tcggctttgg ctttattgtt attagcaaat gcgcctagtg
    10681 ataaaataag cattgaaacc attatgatgc ttataatatt gaatactcta gatttacatg
    10741 aactattcat ataataccct ccatttaaat caaaatattt tacgttgcta agactttgct
    10801 tctattttat ctagacgaac ttaattgtta attaagctgc atctggattg tctgaattat
    10861 tgttatcgcc attgtctgga ttatcagggt tgttagggtt gtcagggtta tttggttcat
    10921 ctgggttgtt agggttatca ggattgttag ggttatcagg attgttaggg ttatcaggat
    10981 tatctgggtt attaggttgg tcatcgttgg caaaatggat atcttcaata ttttgtttta
    11041 agaagttgcg tacattttca ttaataaata ccgcaccatt aaattcattt ggtacaccgc
    11101 cccaatgaat tccaatcact tcattttttt cattaaatac aggtgaacct gagttaccac
    11161 cagttgtact taaatcatat tgcatagctt cgcctttgag gtaagtgatt tttcctttac
    11221 tttcccacat tgttgctaca ggtttatcac caggatatcc tgttacagta atattttggt
    11281 taacttgtgt ttcagcatta ttactcattg ttgctggttt aactacttca ccaatatgtt
    11341 tgttttgctc attaggggag aatttaacta ttgctaaatc accttcgcct gaatatttag
    11401 tgatttgttc agcagtgaaa ccaccatttg gataattgtc ttggttaatt gcagaaggga
    11461 atgcttttaa agcatgagga tcaccgtgcg tagcatctac gacgtgttta tttgttaaaa
    11521 gagtatcttt acctacaact acaccggaag caataaatgt accagtaggt gcttcaactt
    11581 gaatataagt tacgggtgca taatgaccat tcgttgtatc tgtgatttgg tgacgatcgt
    11641 tatttggtaa tataacattt gcgtgttcac gttgttctaa tggtttaagg ttaccgcctt
    11701 tttgaatctt aggtgtttgc tgtttgcttg actgcgtttg ttgtggatga ttgtccatag
    11761 cctttgatga taacgcgttt gctgctggag aactcacaag tgtcgctgtt gtcaaagttg
    11821 caacgaataa agaactaact tttaaaaatt tacctttcat ctaaaaacct ccaaaaaatt
    11881 tatttacaag ttaaatataa cactaaaaat ttttaagtca ataagaatat ataaataaat
    11941 taaaaagttt ttgtaaagat tagagtttac aaaaatatta agggaattaa aattgaattg
    12001 tattatactt tattcaattg agttaaattg ataagatcgt caattgtttg catttatcta
    12061 aaagaataaa taagaaagga attgagagcg aatggaaagt atttgtttat aataataaat
    12121 agtaactttt gaataataat tgtttggcca aaggtagatt aatatgaatg gtgatatatt
    12181 atttgagtgc ttaagtttcg aagaacaatt ataagttagc atattaaaag cttttgatag
    12241 aatcattttg agtgaggtgt ttaaaggaga ggaatataac tcggtagtat ttaca
//
LOCUS       DAHHYJ010000077        12219 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000077 DAHHYJ010000000
VERSION     DAHHYJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.077"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(1..121)
                     /locus_tag="NBK22_001631"
                     /pseudo
     CDS             complement(1..121)
                     /locus_tag="NBK22_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
     gene            272..1270
                     /locus_tag="NBK22_001632"
     CDS             272..1270
                     /locus_tag="NBK22_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2414820.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMMIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            1502..2383
                     /gene="rbsU"
                     /locus_tag="NBK22_001633"
     CDS             1502..2383
                     /gene="rbsU"
                     /locus_tag="NBK22_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCD2414821.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            2398..2802
                     /gene="rbsD"
                     /locus_tag="NBK22_001634"
     CDS             2398..2802
                     /gene="rbsD"
                     /locus_tag="NBK22_001634"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCD2414822.1"
                     /translation="MKKSAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            2830..3744
                     /gene="rbsK"
                     /locus_tag="NBK22_001635"
     CDS             2830..3744
                     /gene="rbsK"
                     /locus_tag="NBK22_001635"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCD2414823.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTTEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFIVAQLEVPIPAIISAFEIAKAHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(3842..3976)
                     /locus_tag="NBK22_001636"
     CDS             complement(3842..3976)
                     /locus_tag="NBK22_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414824.1"
                     /translation="MIIIIINIAYLYAIIWKLKRLQKIVTSFITKHESTSIGLVENYF
                     "
     gene            3995..4756
                     /locus_tag="NBK22_001637"
     CDS             3995..4756
                     /locus_tag="NBK22_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2414825.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYNQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTIKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKED"
     gene            complement(5247..6683)
                     /locus_tag="NBK22_001638"
     CDS             complement(5247..6683)
                     /locus_tag="NBK22_001638"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD2414826.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEGVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDVGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKAHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYQQVIASNGDKL"
     gene            complement(6699..7490)
                     /locus_tag="NBK22_001639"
     CDS             complement(6699..7490)
                     /locus_tag="NBK22_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2414827.1"
                     /translation="MSNKYKEQAQDILTAVGGVENIVDATYDTKCITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDAQLVIKLNGNVDEVYQQLQRLIKNANVEESENTDNINSQDTS
                     YTPQVKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     VSMIVPTKHAVGIQSEDGVDIVIHIGVNTVDLEGKGFKCFVKQNDHVEAGQTLLQFDQ
                     QYIQQQGYNADVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            7639..8343
                     /locus_tag="NBK22_001640"
     CDS             7639..8343
                     /locus_tag="NBK22_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2414828.1"
                     /translation="MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEM
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDKLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(8451..9152)
                     /gene="lrgB"
                     /locus_tag="NBK22_001641"
     CDS             complement(8451..9152)
                     /gene="lrgB"
                     /locus_tag="NBK22_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCD2414829.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(9145..9588)
                     /gene="lrgA"
                     /locus_tag="NBK22_001642"
     CDS             complement(9145..9588)
                     /gene="lrgA"
                     /locus_tag="NBK22_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCD2414830.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(9701..10441)
                     /locus_tag="NBK22_001643"
     CDS             complement(9701..10441)
                     /locus_tag="NBK22_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCD2414831.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSANAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPTYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(10444..12198)
                     /locus_tag="NBK22_001644"
     CDS             complement(10444..12198)
                     /locus_tag="NBK22_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD2414832.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGSFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
BASE COUNT         3950 a         2317 c         1959 g         3993 t
ORIGIN      
        1 aaatatttta ttctttaaaa taatgacttt tgtatttttc atcacaataa acattttaaa
       61 attcttgtta tcataatcat taaaagttat ttaaccttaa taatatattc tctcgcctca
      121 accttaatcg catactatag gcgtctgttt gtagacaata aaagtcattc acgtcttcat
      181 atgtcatcaa atgtttatca tgatatgatg aatataataa tcgggtatat aactgtatga
      241 ttaattacac aataaagaga aggaatataa tatgaaaaaa gtgtcaatta aagatgttgc
      301 tagagaagct ggtgtatcag ttacaactgt gtcacatatt ttaaatcata atgatagtcg
      361 tttttccgca acaacgataa aaaacgtaca tgctgtttca gaacgtttag gctatgcccc
      421 taataaacat gcaaaacaat tgcgcggcag taaaattcaa actattggcg tcattttgcc
      481 tagcttaaca aatccgtttt tctcagcact gatgcaaagt attcatgacc ataaaccatc
      541 tgatgttgat ttatgctttt taacatctac agcaactgat ttgtatgaca atattaaaca
      601 tttaattgat cgaggtattg acggattaat tatcgcacaa tacatttcat ccccggacgc
      661 cctaaataac tatctaaaga aacatcatgt accttatgtc gtactggatc aaaatgacca
      721 tcaaggctat acagattttg ttcggacaaa tgaatatcaa ggcggacaac ttgcagcaca
      781 acatttaata gaactcggtc acaacaatat gatgattgtt gcaccatatg acatgatggc
      841 gaatatgtcg actcgtgtcg ctggatttgt tgatactttg cgcgcgaatc aattaccaga
      901 accacaaatc gtccatactg aattatctaa gcgcggtggg ctaaccattg ttgatgacat
      961 catggttcaa tctgccactg caatcttcgc tattaacgat gaactcgcta ttggcatttt
     1021 acgaggacta attgaacatg gcatcagtat tccgaaagat atctcattaa taggttatga
     1081 cgacattgat tatgcagcgt acgtctcgcc acctttaact actgtggcac aacctataac
     1141 tgatattggc aaaacatctt taaccttatt acttcaacga ttacagcact tagataaatc
     1201 cattgatatg attgaattac caacgacttt aaaaattcgt gcaacaactg gctatcatct
     1261 ttcaaactaa ctacgtatct tccgaaatat actcatcatt gttaggccct tagcgttgct
     1321 ttaatgctga gggtttttaa tcataattat ttcactaaga aattaaaata ataatgtatg
     1381 aatttttaaa tatgatttaa acgttttcag tttttatgaa aacgcatgca ttttgcaaat
     1441 aaaaatggta cgatggcatt ggtaaaacgt tttactaaaa acaaatcgtg aggtgtataa
     1501 catgagcatt gttgcattac ttatcgggtt aggcccctta attggctggg gcttcttccc
     1561 aacagtcgct tcaaagtttg gtggtaaacc tgtacatcaa attatcggtg ctactgtagg
     1621 tacgttaatc ttcgctatta tattagccgt agtcacatca agtggcttcc ctactggaac
     1681 caatttgcta ttcgccttat tatcaggtgc aggatgggga ttcggacaaa tcattacatt
     1741 taaagcgttc gaattagtcg gctcatctcg tgccatgcca gtcacaacag cattccaatt
     1801 attaggcgca tctttatggg gtgtctttgc attaggaaat tggccaggca ttggtcataa
     1861 aatcattgga tttacagctt tagtcgttat tctaattggt gcgcgtatga cagtttggag
     1921 tgaacgcaaa gaagcaagta acgccaaaaa tttacgtcgt gcagtggtac ttctgttaat
     1981 tggtgaattt ggatactggt tatattcagc tgcaccgcaa gcaacttcta ttgatggcct
     2041 aactgccttt ttacctcaag caatgggtat ggtaattgtt gcagtcattt atggctttat
     2101 gaatatgaaa gcagagaatc cattccgtaa taaaattacg tggttacaaa ttatttcagg
     2161 tttcttcttt gcatttggtg ccttaacata tcttatttca gcacaaccta atatgaatgg
     2221 tttagcaact ggatttattc tttctcaaac atccgttgtg cttgctacat taactggtat
     2281 ttatttctta aaacaacata aaacgtcaaa agaaatggtt attacaatca tcggcttagt
     2341 actcatttta gtagccgctt ctgttacagt atttataaaa taaggagtgt agatgtcatg
     2401 aaaaaatcag ctgttttaaa tgaacatatt tcaaaagcaa tcgcgacaat tggtcatttt
     2461 gatctattaa cgattaatga cgctggcatg ccaattccaa atgatcatcg tcgtatcgac
     2521 ctagctgtaa ctaaaaactt accacgcttt attgatgtct tagctacagt gttagaagaa
     2581 atggaaatcc aaaaaatata cttagcagaa gaaataaaag aacataaccc tacacaattg
     2641 caacaaatta aacaattgat ttcatcggaa atcgaaatca ttttcattcc tcacgaagaa
     2701 atgaaaagta acttagctca cccattaaat aaaggtaata ttcgtactgg tgaaacaacg
     2761 ccctactcta atattgcatt agaatcgaat gttacttttt aaaagttata acttgaaagg
     2821 agcgtacaca tgaccaacaa agttgttatt ttaggttcaa cgaatgtcga tcaattttta
     2881 acagttgaaa gatatgcaca accaggcgaa acattacatg ttgaagaagc acaaaaagca
     2941 ttcggcggag gtaaaggtgc caaccaggct attgccactg cacgcatgca agcagacaca
     3001 acatttatta ctaaaattgg cactgatggc gttgctgatt tcatcttaga agattttaaa
     3061 gcggctcata ttgatacatc atatattatc aaaacaactg aagcaaaaac aggccaagcc
     3121 tttatcactg tgaatgcaga aggacaaaac accatctatg tttatggtgg tgcgaatatg
     3181 acgatgacac ctgaagatgt tattaacgca aaagacgcta taatcaatgc agactttatc
     3241 gttgcacaat tagaagtacc catcccggct attatatctg catttgaaat tgccaaggca
     3301 catggtgtga cgacagtatt aaatcctgca ccagcgaaag cattacctaa tgaattatta
     3361 tcattaatcg atattattgt gccaaacgaa acagaagccg aattgttatc tgggattaaa
     3421 gtaactaatg aacaatctat gaaagacaat gccaattact ttttatcttt aggcattaag
     3481 actgttttga ttacgctagg taagcaaggt acatattttg ctactaaaaa tcaaagccaa
     3541 cacatcgaag cttataaagt aaatgcgatt gatacaactg ctgcaggcga cacatttatt
     3601 ggtgcatttg tcagtcgctt aaacaagtcg caagataact tagcagatgc tattgatttt
     3661 ggtaataaag cgagctcact cactgtacaa aaacacggcg cgcaagcatc tattcctcta
     3721 ctagaagaag taaatcaagt ttaaatgaat caaacacagc tatgatatga aggtttagca
     3781 tataacatgc aacattcgta tatcatggct gtgctttttt atctttataa aacatcatct
     3841 attagaaata attttccact aaacctatgc ttgttgactc atgtttagtt ataaatgaag
     3901 tgacaatttt ttgtaatctt tttaacttcc aaattatcgc atataaatat gctatattaa
     3961 tgataataat tatcaattaa aaggaggtta tgctatgtct aaagaagctg gtcatacatt
     4021 tttagctaaa ttaggaaaaa ctcgtctacg ccccggtggt aaagaagcaa cagattggtt
     4081 aatacaacaa ggggcatttt cacaagataa acaagtgtta gaagtggcat gtaatatgtg
     4141 cacaacatct atttatctag ctcatacata tggctgtcac attcaaggcg ttgatataaa
     4201 taagaaagca ttagaaaaag cacaggaaaa catttcagca gcaggtcttg aatcatatat
     4261 tcaagttcaa caagcgaatg ctgttaaatt gccctttgat gacaatcaat tcgatatcgt
     4321 tttaaatgaa gcaatgttaa caatgttacc catcgccata aaggaaaaag cattacgcga
     4381 gtactaccga gtcttaaagc ctgggggtat cttgttaaca catgatattg tcatcgttaa
     4441 tgaatcacat gccacacatg ttgttaaatc attatctgca gcaattaatg tcaatgtctc
     4501 accgcagacg aaacttggct ggttagattt atataatcaa gctggtttta atcatgtgca
     4561 ttatcatact ggtccaatga gtttaatgac accaaaaggt ttaatttatg acgaaggtat
     4621 tgttggaact ataaagatta tcaacaatgc tttgaaaaaa gaaaatcgac caatgttttg
     4681 taaaatgttt aaaacgatga ctaaattgcg taaagatatg aattatatta cttttgtcgc
     4741 taaaaaagag gactaaatat aatgccacta accgtacttt gtatctatgt ttgactgcta
     4801 ctttaatttc tttgtgacac taatcatcta cttaacaata tcgttatcgt tgattagtaa
     4861 gtcatcaatt ttggttaaag actttcataa acactcaaac attaacacta tacatactta
     4921 gttagtggca ttattttttt aaaaatttta acatcacggg attggaacag aaatgatatt
     4981 ttcgcaaaat ttatttctgt ccttccccaa cttgcattac ctgtagaatt tcttttcgaa
     5041 attctctgtg ttggggcccc gccaacttgc acattattgt atgctgactt tccgccagct
     5101 tctatgttgg ggccccgcca acttgcatta cctgtagaat ttcttttcga aattctttgt
     5161 gttggggccc gccaatataa cactgtaggt cctactatat taatttatgt ctcgggctct
     5221 aaccctaaaa tacttacaat gtacctttat aatttatctc catttgatgc tatcacttgt
     5281 tgataccaat agaacgaatc ttttttcaag cgttccatcg tgccatgacc atcattatct
     5341 cgatcaacat atattaaacc atagcgtttc ttcatttcac cggttgtaaa tgaaacaata
     5401 tcaatgattc cccatggcgt ataaccgatt aaatctacac catcttgatc aactgcttct
     5461 atcgctgctg taatatgtgc ttttaaatat tcaatgcgat aatcatcatg aatatgtcca
     5521 tctaccactt catcaactgc accaaaacca ttttccacaa taaaaagtgg caactgataa
     5581 cgatcatata acacattcaa tgtatatctt aagccatctg gatcaatcgc ccaaccccaa
     5641 tcactcgttg cgatatgcgg attctccaca gaatgattca aaccaccgtt gacgatgttg
     5701 ttttcaactg tagtatcaac atcatgtttt acagccgtag acatgtaata actaaagcca
     5761 atataatcaa ccgtgccctg ctgtaaaata ctgtcgtccc cttcttgcca tccaacatca
     5821 tatccttttc gtgccaacat ttttttagca tagcttggat aataacctct cacttggaca
     5881 tccgggaaaa agaaacgtaa gcgattcgca atttgtgctt ccatcatatc tttcggatga
     5941 cacgaatatg gataaatggg tacatgtgaa atcattgttc caatcttaaa cttcggatta
     6001 atctctttac caagacgaac tgctaaagca ctggctaaaa gttcatgatg tgctacttga
     6061 tacaagactt cttcaggatt atcattttct gtcaatgcta ccccagaatt cgtccataaa
     6121 aagataggat ttgatgtgtc catctgatta ttaatttcat taaacgtcat ccaatatgtc
     6181 actttatctt tatatctttc aaatacaaca cgcgcaaaat gcacaaaata atcgacaact
     6241 tctctatttc taaatccacc ataatgtttc gctaaatgaa gtggcatctc aaaatgtgat
     6301 aacgtcacaa caggttcaat accttgtgca attaattcat caaagatacg atcataaaag
     6361 gcaagtcctt cttcatttgg cacaccttca tccccattcg gaaagatacg tgtccacgca
     6421 atcgacgtac gtaaacattt caatcccatt tctttaaaca aggcaatatc ttccttataa
     6481 cgatgataaa aatcaatacc ttcatgattt ggataatagt gattgggatc tatagattct
     6541 gtaatctgac gtgctttgcc atgtgcacca ctcgtcataa catcaattac acttaatcct
     6601 ttaccacctt tatcatatcc accttcaaat tgatttgcgg caagagcgcc accccacata
     6661 aaattttgcg gtaattttgt cattattaca acacactcct agtttttaaa tattttaaaa
     6721 ataacatctt cagtcgtaat gatttcattc attgtcagtt ctacttttcc taaatcggca
     6781 gagttgctaa taacgacaat aacgtcagca ttgtagcctt gttgttgtat atattgctgg
     6841 tcgaattgca acaacgtttg ccctgcttca acatgatcat tttgctttac aaagcacttg
     6901 aaccctttac cttccaagtc aactgtattc acgccaatat gaatgactat gtccacaccg
     6961 tcttctgatt gaataccaac tgcatgctta gttggtacaa tcatagatac taaaccattg
     7021 aacggtgcga ttactttgga ttcttcatga gctttaattg ccaagccttc gcctaccatt
     7081 ttctctctaa aaattgaatc tcttacttct ttaagtaaaa tacgacgacc agcgattggt
     7141 gctttcacta aaattggtgt tgttactttt acttgaggtg tataacttgt atcttggcta
     7201 ttaatgttgt cagtattctc actctcttca acattagcat tcttaattaa tcgctgtaat
     7261 tgctgataca cttcatcgac atttccattt aattttatga ctaactgcgc atcattttct
     7321 gctacagatg tcacatcaac tatttgtttc acttcatttg ctgtagaagg gattgtatgt
     7381 tgcatatgaa ttgtaatgca cttcgtgtca tacgttgcat caacaatgtt ttcgacacca
     7441 cctacagctg taagaatgtc ttgggcttgt tctttatatt tattgctcat aaatattacc
     7501 ccctcaactt gatagtagaa tgaaaatgta tttacttacc tccaattata attgtaccgg
     7561 ttcaatttgt aaacgccgat acaattataa tattttgtgc tataataatt acagacaaag
     7621 tgaaatgagg acagaatatt gttaaagtat gaacatattg ctaagcaact taatgcgttt
     7681 atacatcaat ctaatttcaa acccggtgat aaattgccaa gcgtgacgca attaaaagaa
     7741 cgttatcaag taagtaagag tactatcatt aaagcattag gcttattgga acaagatggt
     7801 ttgatctatc aagcacaagg cagtggtatt tatgtgagaa atattgcaga tgccaatcgt
     7861 atcaacgtct ttaagactaa tggtttctct aaaagtttag gtgaacatcg aatgacaagt
     7921 aaggtacttg tttttaagga gatggcaacg ccacctaaat ctgtacaaga tgaactccaa
     7981 ttaaatgcag atgataccgt ctactattta gagcgattaa gattcgtgga cgatgatgtt
     8041 ttatgtatcg aatattctta ttatcataaa gaaatcgtga aatatttaaa tgatgatatt
     8101 gctaagggct ctatcttcga ctatttagaa tcaaacatga aacttcgtat tggtttttca
     8161 gatattttct ttaatgtaga taaactcact tcaagtgaag cttcattact acaattgtct
     8221 acaggtgaac catgtttacg ttaccaccag actttttata caatgactgg caaacccttt
     8281 gattcgtctg acatcgtatt tcattatcgt catgcacagt tttatattcc tagtaaaaag
     8341 taatacatac ataaaaacgt ctatatccca gttataaacc ggagtataga cgttttttca
     8401 cgataataac aatggctcaa actgctatta tcttgcttag gtttttcgtt ttagaagaat
     8461 attgctacaa agacaggcac aactgctaca acaactacac caactaacac taaagctata
     8521 cttgccattg attcttctac aggtcctaat tctttggctg gtgctacacc taatgtgtga
     8581 ccacttgttc caagtgctaa tcctcgggca atagggttag taattcggaa aagcttcaag
     8641 aatttattac ctagggcata aataatgaca ccatttaaaa taactgctaa tgatgttaat
     8701 tcttttatac caccgatacc agctgatact ggtaacgcaa tcgctgtagt tgctgcttga
     8761 ggtaacattg ataaaataac atcattggca aattgtgcta acttcgcaaa agttaaaata
     8821 attaataacg ctacaactgt accgatacca atacctccga tgatacgatg ccaatgttta
     8881 acaagcactt cacgcttttt atataacgga atcgcaaaac agattgttgc tggttctaag
     8941 aagaagtaaa taatgtctcc acctattttg taagtcttat acggaatgcc tgttaaatag
     9001 aggaaggcca caccaaatac catactgaca aatagcggtg caaataagaa gaaacgattt
     9061 gttttttcaa ataatatggt cgctaagaaa aatggtataa cggataacag tattccgaag
     9121 taaggtgtgt ttagtgctaa gtggttaatc atgagcttgt gcctcctcta ttttgatctt
     9181 ttttgtgact ttgtcacctt tagatctcga agtaactttc ataataattt gtgtgacata
     9241 gccagtacaa ataagtaata gtattgttga gacgattatt agtccaatga ttaaaaatgg
     9301 tgcttggcta atgacaccta aagagttaac aactgagata ccggctggta cgaagagtaa
     9361 gccaatgtta tttgttagtg tcgttcctac tttttcgact tcgcctaact taacagcacc
     9421 agtacataat aatacaaata ataatactaa accgattact gatgcaggca taggaattgg
     9481 cataaatgat tcaattattt tcgatacaaa gagtactaaa gcaattacaa tgacttggtg
     9541 aaaaaagtgt gctggttttg atgcgtcttt ttgttgtttc acgaccattg cctcctacgt
     9601 ttgatttaac taaagtatag atggctcact tcgatttgcg tgaattttag tccgaaatac
     9661 aaaatatcat aggtaaaatg cataaaaaaa aggattactg ttaaagtaat cctatcgacg
     9721 ctttaaaatc tttcataaat gaacgtccaa cttgcatctt gacaccattt gtcaatatta
     9781 ccatataagt gtagttaaac cattgttgca cttctttaat gtgtttcgtg ttaataatat
     9841 atgaacgatg aatacgtata aaataagtgg gattcaatcg tttttcataa cgattcaatg
     9901 gctctgttgt ttcgtattta tgattcgttg tatgtatggt tgtaatacca ttatgtgtgc
     9961 caatcccaat aatattttgt tgctttaaca tgtgaatttt atcgtcaatt tcaacaggta
    10021 aactttgatc aaaattcgcc gacatatcat tcgcaattgc atttgcgcta ttatcatctt
    10081 tggctttagt cgcacgaact ttattgactg cttgttcaat acgtttttga ccaaacggtt
    10141 tcaaaatata gtctgtcgca tttaattcaa atgcctgtac tgcgtattgg tcatgtgcag
    10201 ttgcaaaaat aatcgcaggt ggctctttca tcttttgaat cttagctcct aattcgatcc
    10261 cattttcatc cattaaattg acatctaaaa atataatgtc atattgattg atcagtagtg
    10321 cttccaatgt ttcttttaca ttttctgcct cattaatttc ttcaaaacca ccaatttcat
    10381 ttaataaata tgttaattca ttacgtgcta atggctcatc atctatgatt aatgctttca
    10441 tatttattcc tcctcttgtc tttcataagg aagtacacac caaaaagtgg taccgctcga
    10501 tgtcgattca aattgtaatg ctgcggattt tccaaataat ccttttaggc gtaagtttaa
    10561 attttctaaa gcactaccag ttccagattc tgattctaca gatgtttctc ccaacaaatg
    10621 cattttatct ttagaaatac cctgaccatt atcttgtaca ataatacgta catgtgttgc
    10681 agtttcttta atcactgaca cgtcaatatc gttaccttgc tttcgattcg taaacgcatg
    10741 tttgatggca ttttcaacta aaatttgaat taaaaatggt ggtacaagca catcgcgata
    10801 tttgtcttca acattaatat tgatattaaa tcttcctgga taacgtgctt gttcgagtga
    10861 taagtatgca cgcacttgac ttaactcttt atctaaagta atcgtatgtt gctttgagcc
    10921 ttgtaaattc gctctgaaaa aataactcaa ttctaatagt aactctcgtg ccttttcgct
    10981 atttattcta actaaagctg aaatcgtgtt aattgaattg aagaaaaaat gtggactcac
    11041 ttgtgcctgt aatgacttaa tctcagcatc tttcaataac ttactttgcg tttcggcttc
    11101 accaagttca atttggctac taaaaatatt tgccaatcct tctgcaagtt gacgttccac
    11161 aaaagttaaa tcattagggt ttgtaaaata catcttcaat gtaccgacga tagaaccatg
    11221 catctcaagt ggtatcacga tagctgctct aagcgggcaa ttcggatgac tacaaccaat
    11281 ctcttcttta gtatgaactt ctttcaactt tcctgatttc aatacatctt tagacagact
    11341 tgttaatatt tcatttgttg gtatgtgatg atcactacct gcacctacat gcgataagat
    11401 ttcatttttg cttgtaattg ctacggcaga tactttcatt aaatttttaa taatcatcgc
    11461 aatttgttgt gccgattctc tattcaatcc ttctttaaaa tacggcaatg tctggttcat
    11521 caattgcagt acgtcatgtg tttgaacagc cttcatttgc tcttcttgct ttaatgttga
    11581 aatgataata gacataaaaa tcgccgtacc aacgctatta acaataatca ttggtagtgc
    11641 aattaatgat atgaggtcaa ccgcatatgc tttgtcgtgg gaaaatgtta aaatgctcaa
    11701 catttgaatc atttccataa caattccaat catggcactt ttcgcaatac tcgggtaacg
    11761 cttgcgtctt tgagcttgta agccaaaata accagcaatt ataccgataa atatagatga
    11821 gataagataa acttgtgcat ccgccccacc catatacact ctgaaaatac ctgaaataac
    11881 gccaacaaat agacctacaa aagagccacc aactaatcct gcgacaccta tcgttaatac
    11941 acgtgtgtta gctaaagata catcatcatc taaacggaag tacacacttc ctgacaaact
    12001 atgttgatga tcgatgacga taccagttaa attagacatt aaggcaaaca aactgaaaat
    12061 aatacataat tgccaacgtg ctttccatgt acgtcgacga ttcattaagt ttttaaaata
    12121 tggaatattc atcaacacat aggccaaaat aataattaaa cctacacgct caagtaataa
    12181 cattgttagc gatagcacga tagcacctca gtgaattat
//
LOCUS       DAHHYJ010000078        12208 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000078 DAHHYJ010000000
VERSION     DAHHYJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.078"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            62..1480
                     /locus_tag="NBK22_001645"
     CDS             62..1480
                     /locus_tag="NBK22_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCD2414833.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIALAEKFMIGI
                     FVIALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(1893..2801)
                     /gene="rarD"
                     /locus_tag="NBK22_001646"
     CDS             complement(1893..2801)
                     /gene="rarD"
                     /locus_tag="NBK22_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCD2414834.1"
                     /translation="MNTEYKKGIFLALSAYILWGILPIYWQFIDAIGAFEILAFRIIF
                     SAIFMIFILAVGQKQRNAFQRDKNQLLGKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            3022..3596
                     /locus_tag="NBK22_001647"
                     /pseudo
     CDS             3022..3596
                     /locus_tag="NBK22_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            3761..4777
                     /locus_tag="NBK22_001648"
     CDS             3761..4777
                     /locus_tag="NBK22_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCD2414835.1"
                     /translation="MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSR"
     gene            5020..5814
                     /locus_tag="NBK22_001649"
     CDS             5020..5814
                     /locus_tag="NBK22_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCD2414836.1"
                     /translation="MNIAKLENYLQIDSSRYNRPSIEALNYYATRFMLTVPFENIDVQ
                     NSKPISINIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHAIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTHPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTRNGSSEKFDVTKDNYKHFLEKYFGLNVTINRIEKQ"
     gene            complement(5948..6460)
                     /locus_tag="NBK22_001650"
     CDS             complement(5948..6460)
                     /locus_tag="NBK22_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCD2414837.1"
                     /translation="MEKGNQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIIYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSISGLFTFSGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            6743..7501
                     /gene="vraD"
                     /locus_tag="NBK22_001651"
     CDS             6743..7501
                     /gene="vraD"
                     /locus_tag="NBK22_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCD2414838.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7491..9371
                     /gene="vraE"
                     /locus_tag="NBK22_001652"
     CDS             7491..9371
                     /gene="vraE"
                     /locus_tag="NBK22_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCD2414839.1"
                     /translation="MTFNHIVFKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLELEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFQSALFLKRRSILSMMKDSIKTDVTTAKVTVIEVISGVLGIAMIALGYYM
                     ATEMFGTFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKQNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNYYETITVNNVKDQVITLENGSDSGRTNSILSANNKVTGN
                     NAIITNTKSLPNVINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVVEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKKKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            9464..9655
                     /gene="vraH"
                     /locus_tag="NBK22_001653"
     CDS             9464..9655
                     /gene="vraH"
                     /locus_tag="NBK22_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCD2414840.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALAMIVIVLSVVTTPIIGI
                     PAGLLGGAYYLKRREEKGK"
     gene            10048..10248
                     /locus_tag="NBK22_001654"
     CDS             10048..10248
                     /locus_tag="NBK22_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD2414841.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(10366..10935)
                     /locus_tag="NBK22_001655"
     CDS             complement(10366..10935)
                     /locus_tag="NBK22_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2414842.1"
                     /translation="MNLSKQSKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKAQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGVTVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVIGILSLIIFLSVYLANKFL"
     gene            complement(11167..11562)
                     /locus_tag="NBK22_001656"
     CDS             complement(11167..11562)
                     /locus_tag="NBK22_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCD2414843.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(11630..11983)
                     /locus_tag="NBK22_001657"
     CDS             complement(11630..11983)
                     /locus_tag="NBK22_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCD2414844.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
BASE COUNT         4129 a         2128 c         1892 g         4059 t
ORIGIN      
        1 gtagttagct ttattcttaa attgaaatat gtataaaaca tagaaagaag gacgttttaa
       61 catggaaaac acggttaaat atcgtaagtt tatactccct atcgttgtag gtctccttat
      121 ttgggcactt acacctttta aaccggatgc tgtggatcca acagcatggt atatgttcgc
      181 aatattcgtc gcgacaatca ttgcttgtat tacacaaccg atgccaattg gggccgtctc
      241 tataattgga tttacaatca tggtactcgt tggcattgtt gacatgaaaa cggctgtcgc
      301 tggttttggt aataatagca tttggttaat tgctatggca tttttcattt cgagaggatt
      361 tgtgaaaaca ggtcttggta gacggatcgc acttcatttc gtcaaattat ttggtaaaaa
      421 aacattaggc ttagcatatt ctatcgtcgg tgtagattta attctagcgc ctgctacacc
      481 gagtaatacc gcgcgtgctg gtggaatcat gttcccaatt atcaaatcac tttctgaatc
      541 atttggttcg aaaccgaaag acggatcagc acgcaaaatg ggtgcatttc ttgttttcac
      601 agaattccaa ggtaatttaa ttactgcggc tatgttttta actgcaatgg ccggtaaccc
      661 ccttgcacaa aatttagcat ctagcacatc taatgttcac attacatgga tgaattggtt
      721 cctagctgct ttagttcctg gacttgtttc cttaattgtt gtacctttta ttatttataa
      781 aatttatcca ccaactgtta aagaaacacc aaatgctaag agttgggctg aaaatgaatt
      841 agcgactatg ggtaaaatcg ctttagctga aaaatttatg attggtattt ttgtcattgc
      901 tctaacatta tggattgtcg gaagtttcat tcatattgat gcaactttaa cggcctttat
      961 tgcgctagca ttgttattat tgacaggtgt cttaacatgg caagacattt taaacgaaac
     1021 aggtgcttgg aacacattag tatggttctc agtattagtg ttaatggccg accaattaaa
     1081 caagcttgga tttattcctt ggttaagtaa atccattgct acaagtcttg gtggcttaag
     1141 ctggcctata gtcctggtca ttttaatatt gttctacttc tattcacatt acttatttgc
     1201 aagttctaca gcacatatca gtgcgatgta tgcagcatta ctaggcgttg ccatcgcagc
     1261 cggtgcacca ccattattca gtgcattaat gttaggtttc ttcggtaacc tattagcttc
     1321 aacaacacac tatagtagtg gcccagcgcc gattctattc tcttcaggtt acgtgactca
     1381 aaaacgttgg tggacaatga acttaatatt aggtttcgtc tactttatta tctggattgg
     1441 tttaggatca ctttggatga aagtaattgg tatattttaa aatatttaaa ttagtgctcg
     1501 aatctcattg atttgggcgc tttttaattt gtatttaaaa tcaacctttg ctaaatcaag
     1561 actccctttt taaaatacgt ttatccttta aatcattgcg tgcttcactg aaaatttgta
     1621 taaagattta agtcattacg taacatcaca taaaatacat ttctatacta ttccgcttca
     1681 ttgattaaca ttacgtatgc cctcataaat catcatacaa aaaacacctt cgtttaaatt
     1741 cattttaatt gcgaattcaa cgaaagtgcc ttatttcata tttaatgttt caaatttata
     1801 cgtctgtcac tgttactgca cacatacctc agtctcacct catcatgaca caaaggatat
     1861 tgaaaacacg tataattatc tttcctcgta ttttataggg tttttgcgac cggatgtttc
     1921 ttcaatttaa tgtattgaga aagactatat aacacaatac ctgtccaaat aaatataaac
     1981 gtaattaatt gatctatact aaaaggctct ttgaaaacaa atatgccgag tacaaacatt
     2041 attgttggtc caacgtattg aataaatcct attagcgaaa gtggaatacg ttttgccccg
     2101 gctgagaata ggattagtgg tattgccgta atagcaccag aaaataacaa ccaaaatgat
     2161 gacatgttca atccaaatga catctgatgt tgctgccata aataaataac gtatattagt
     2221 ccagcaggtg cggtaacaat acattcaatc gtaatactgc tgatggcatc aatatgtact
     2281 acttttttca ataatccgta tgtaccaaag gataacgcta atataataga gacgattggg
     2341 aattctccaa tcttgagcgt catatataat acaccgatga atgcgaataa aatggctagc
     2401 cattcaaatt tattgaatct ttcttttaaa aagataagtg cgagcaaaat gctaacaagt
     2461 ggatttatat aataacctaa acttgtttgt aggacgtgac cgttcgttac agcccaaata
     2521 aatgtacccc aatttaatgt aatgacatag cctgctacga caatcgctaa tagctgaatg
     2581 ggcttgccta acaattgatt cttatctcgt tgaaatgcat tgcgttgttt ttgtccaacc
     2641 gcgagtatga atatcatgaa tattgctgaa aatataatac gaaaggctaa aatttcaaat
     2701 gcgcctattg catcaatgaa ctgccaatat ataggtagta ttccccacag aatgtatgca
     2761 ctgagtgcta aaaatatacc ttttttatat tctgtattca cttacatacc tccttactct
     2821 cccaattttt aacatgttac gctcacttag ttaagctaat ataacgattt tactaataat
     2881 atttcgataa agatatcatt ttgtttatat ttcccacatt tattcaccaa ccactaaaca
     2941 atattaattt tataaataat tctgtacaaa tcagggtata ttgccagaaa gactaccata
     3001 caacataaag gatggataca aatgacttta cctaaaattg gaaagcctgc aacacgcgcg
     3061 ctaaattcac aaggtatata cacattagaa gcagtatcac aatatacgaa gtcatctcta
     3121 atggagatgc atggcgttgg tcctaaagct atatcaatat tggaacaagc tttatttcag
     3181 caccaactac attttaaaac ggaagttcag tcttcattac cttttaagtt aacaggagat
     3241 gtgtcatgca atcatgcgcc taaacgtcaa caaatgatag attttatagt tgctacagca
     3301 gcattagata ttgaattatt acgttcactg gttacaacag aatttatttg gtctgtccct
     3361 ggccgctttg acatatacgg acctcaaata ttaattcaag aactatctaa tcattacaat
     3421 caggttgcta gtctcaatat ccattccagc atcacacatg ggtgcttagg ttccatgcat
     3481 ggtattgaaa tattaaaaac tggtaaagag attcattttg ctcatttttt gaatttgaaa
     3541 atcataaaaa ggatgccaag ttaagcaaag tgacatctta cattgttgtt ggttaattta
     3601 tcaaattata tatatcgcaa aaaatctttt gtggttacat gtatattttt atataacaaa
     3661 taattttgca taacattttc gttatatgaa cacttattac tagagtgcac attgaacata
     3721 cagtatgatt aaatatcatg ttatgaaaga aggatatatt ttgactgttt ttaaaaatga
     3781 gcgcttaagc tggttaccat acatagctat tgtcattttg ttacacgtta ttgggtttag
     3841 ttttttatgg attgctggaa aagaccatca tatcttattt ggtatgggga ttcttgcata
     3901 tacattaggt ttacgtcatg catttgatgc tgatcatatc gccgcgatag ataatacggt
     3961 tcgcaaatta ttacagcaac gcaaagatcc atctggtgtg ggtttctatt tttcaattgg
     4021 acattcatct gtcgtatttt taatggctgt ttttttaggg gtatctgtaa aatgggctaa
     4081 agatgaatta ccgcatttcc aagatattgg tggaacgatt ggtacactag tttcaggttt
     4141 ctttttagtg cttatcggtg tgttgaatct aattatttta atctctttaa ttaacttgtt
     4201 cgctaaatta cgtcgtgaac acattgaaga agctgaagtc gatgcattac ttgaatctag
     4261 aggattggtt tctcgatttg taggacctta tttcaaatta atcacgcgta gttggcacgt
     4321 attgccactt ggctttttat ttggacttgg ttttgataca gctagtgaaa ttgcgttact
     4381 cgctctttct tcaggcgcat cacaacaagc catttcattt atcggaatct tatctttacc
     4441 aattttattc gcatcaggta tgagtttatt ggatacatta gatggtgttg taatgaagta
     4501 tgcctataat tgggcattct tcaatcctat tcgcaaaatc tattacaata taacgattac
     4561 tgcgatatca gtcatggcag cattagtgat tgggatgatt gaattgctac aaattcttgc
     4621 tgataagtta gatttacatg gtgcgttttg ggcattcatt ggttcgatcg aatttgatta
     4681 tttaggctat attttagttg cattattttt aattacttgg cttatttcaa gtttaatttg
     4741 gaagtttggt cgcatagaac acaaatggtc tagataaggc aacttgtctt gcaactacct
     4801 ttgtattgtt gcatttttag acaccgtgca cacttcttcc attatgctac gctctcagta
     4861 gtcatcttta aatcgtttct tttagagatg acttcatttt gagagcgtat ttttctattt
     4921 aaaatgtatt tatccaaaat tcaatgcttt cgaatttaaa atttcagtat ttctgtttaa
     4981 aatttaatta taatctttaa tacaggaggc gctatgctta tgaatattgc gaagttagag
     5041 aattatttac aaattgattc atctcgttat aatcgaccga gtattgaagc attaaattat
     5101 tatgcaacac gttttatgtt aactgtacct tttgaaaata ttgatgttca aaatagtaag
     5161 ccgatatcta taaatatcga tgcacttttt aataaaattg ttcatgataa acgtggtggt
     5221 ttttgttatg aattaaatac atttttcaaa gcctatttac aacaaaaagg atttaatcct
     5281 gaattaatgt cagctactat tcacacaccc ggtggcggtc gtagtctgaa cggttcacat
     5341 gcttcacttg ttgtttctat aaatgatgta ttctatgtaa ctgatgttgg ctttggagac
     5401 ttacctctac atgccattcc tattacttca tctgagcata cacaaccaat tactgatatc
     5461 agtggtacat tccgcgctat ttttaacaac gaagacaagg atattttcta tgttcaaaaa
     5521 ttcgaaaatg atcattggca tacaaaatat gaagctgaat tcaaacctaa acaaatagaa
     5581 gattttaata gcaacatcga atataatcaa acgcatccag attcaatatt tgtacagcat
     5641 ttattaatta caatgccaca atcatttggc cgtgctacaa tgtctgaaaa tcatttaact
     5701 ttaacaagga atggtagctc agaaaaattt gatgtcacta aagataacta taaacatttt
     5761 ttagaaaaat attttggact aaatgtaact attaatcgta ttgaaaaaca ataatagtaa
     5821 aatataaaaa tatgggcgta tcttacttat tgtaggtagc gccccttttt tatgcttcat
     5881 aaacacaaca aaaaggatat gacacaaact tcatgtcaca cccttctttt gaaccgcttc
     5941 attttactta agcaagtgca tctacaacat aaatgacacc tacgaataca aatgcaatag
     6001 caatcatcgt cgaaataaag acgataccca ttaatggatt aaataataaa atgacaccga
     6061 aaacaatacc taataaatta aaaatcactg aaattagttt taagctacca ctacccgaaa
     6121 acgtaaataa tccagagata gaactaaaaa taaaccaaaa agcaaacata taaataaaga
     6181 atgccgagct agcgccaaca ttaaaaatga caataagacc aaagagaata tctacaatcc
     6241 ccataaacag aatccaattt tggttaccac ctactaaagc ttttgctttt ctacggtaaa
     6301 tgatttgaat cacaccgtta attaatacaa acagtccaat caaccaggta atagcataaa
     6361 aattttcaat tggaaatgta aaaataacga ctgccaacat taataataat acacccatta
     6421 ttaaactaga ccatttaata ccttgatttc ctttttccat aacaatcaat tcctttcaaa
     6481 taaagccctc ttttaatttt tacaaaaaat ttattataca ataattaaat tcccttttct
     6541 caatttacac aaacatcatc acttagaaag caccattgct tttgtctaat atgtacgatt
     6601 aatctgtatc ctcactcaaa aatctactaa gtagcgatag ctttcaactc tgtaaggttc
     6661 attcattgaa ttgtaagttt agataaaggc tactactcca aacattattt aaaatgaaat
     6721 taaatacaaa aggagtgaga ctatgacaat attatcagtg caacatgttt caaaaacata
     6781 cggtaaaaag cacacatttc aagcacttaa agatattaac tttgacatac aaaaaggcga
     6841 attcgttgcg attatggggc cttctggatc aggtaagaca accttattaa atgtactaag
     6901 ttcaattgac caaatttcta gcggtagcgt gattgctaac ggacaagagc ttaataaact
     6961 taatcaaaaa gcacttgcca aattccgtaa agaatcatta ggtttcatct tccaagatta
     7021 cagtattctg ccgacattaa ctgtcaaaga aaacattatg ttacctttat ctgttcaaaa
     7081 aatgtcgaaa gcaacaatgg aagaaaatta taaagcgatt actacagcat taggtattta
     7141 tgacctagga aataaatacc ctagcgaatt atctggtggt caacaacaaa gaactgcagc
     7201 agcgagagca tttgttcaca aaccacaaat catatttgca gatgagccaa caggcgcact
     7261 cgactcgaaa agtgcaaatg acctattaca acgtttggaa gaaatgaata aatcgtttga
     7321 tacaactatt gtcatggtta cacatgatcc ggttgcagca agttttgcag aacgtgtcat
     7381 catgctaaaa gatggccaaa ttcatacaca actttatcag gaaggacgtt ctaaacaggc
     7441 cttttatgaa gacattgtac atcttcaatc agtattaggt ggtgtctcaa atgacattta
     7501 accatatcgt tttcaaaaac ttacgacaaa acttaaaaca ttatgcaatg tatttatttt
     7561 cattattttt tagcatcgtc ttatatttca gttttacaac cttacagttt actaaaggtg
     7621 taaataatga cgactcgatg gccatcatta agaaaggtgc tttagtcgga tcaatatttt
     7681 tatttatcat tattgtcatc tttttaatgt atgccaatca tttattcgtg aaacgtcgta
     7741 cacgtgaatt tgcgctattt cagttgattg gtttaacacg acaaaacatt ttgaaaatgt
     7801 tagaacttga acaaatgatt gtatttttaa tcacaggtgt tgtcggtgta ttatgtggca
     7861 ttgcaggtgc acaattattg ctttccatag tatctaaatt gatgtcgtta tcgattaact
     7921 tatcgataca tttcgaacct atggcccttg ttttaactat tttcatgcta attattgcgt
     7981 atgtactgat tttatttcaa agtgctttat ttctaaaaag acgtagtatc ttatcaatga
     8041 tgaaagactc aattaaaact gatgttacaa ctgcgaaagt tacagttata gaagtcattt
     8101 caggcgtatt aggtattgct atgattgcac taggctatta tatggcgaca gaaatgtttg
     8161 gaacatttaa agcactaact atggctatga catcaccgtt tatcatttta tttttaacgg
     8221 tagtaggcgc ctatttattc ttcagaagtt ccgtgtcact aatttttaaa actttgaaaa
     8281 aatctaaaaa tggacgcgta tctattacag atgttgtatt tacatcgtct attatgtacc
     8341 gaatgaagca aaatgcgatg tctttaacta tcattgcaat tatttctgca gttacagtaa
     8401 ctgttttgtg ttttgcggca ttatctaaat caaatacaga tcaaaccctt acatctatgg
     8461 caccaaatga atttaacgtg gtcgctactc aagatgctaa acaatttgaa actaaactaa
     8521 gccaacaaca aattacattt tctaaaaatt attatgaaac tatcactgta aataatgtta
     8581 aagatcaggt tattactttg gaaaatggta gtgattcagg tcgcacaaat tcaatattaa
     8641 gtgcaaataa caaggtaaca ggcaacaatg ccatcattac aaatacaaaa tcgctgccta
     8701 acgtgattaa tattcattta aacaaagatt tagtagtaaa aggtactaag aatgaaactt
     8761 tccgtgttac acaagaagac aaaggtaagg tttatccttt aaatctaagc ttcaactcac
     8821 ctgtcgtcga agtaagtcct gaaaagtatc agcagttgaa aacacaaaat aacgttcata
     8881 ctttttatgg atatgatatt aaacaaacat cacaaaagaa aaaagctcaa gcaattgcaa
     8941 aacagtttgg agacaaagtc atcacgtatg atgaaatgaa aaaagaggtc gatgctacta
     9001 acggtattct aatatttgtt acatcatttt taggattagc atttttagta gcagctggat
     9061 gtattattta tattaaacaa atggatgaaa ctgaggatga actaagtaac ttcagaatat
     9121 taaaacgcat aggctttacg catacagata tgcttaaagg attattacta aaaattactt
     9181 tcaacttcgg tttaccatta ttgattgcta tattacatgc agtattcgcc gcaatagcat
     9241 ttatgaaatt gatgggtaat atttccttca tgccggttat tgtagtaatt gttgtataca
     9301 cactcattta cattactttt gctttaattg cttttgtaca ttcaaacaaa ttgattaaga
     9361 aaaccattta acacttatga ttaaaagatg cttcattgag attttgattt taagcaatgc
     9421 agtataacta ttaactgatt tataaaaaag gagagattga atcatgaaat ttagagaatt
     9481 agtaaaacaa tcatacgagg atttgaaaaa tttgactgta acttggtata acgtcattgc
     9541 tttagcaatg attgttattg tcttaagcgt agttacaaca ccaattatag gtattccagc
     9601 tggtttatta ggtggcgctt attatttaaa acgtcgtgaa gaaaaaggta aataagcaca
     9661 ttccatcggt atagaacatt gctatgacct ttcaagtcag attttaatat caacaagtag
     9721 cgtttatcgc tgcttgtttt ttatggattg cttataaaat catcatgttt aaacgatatg
     9781 tcactttgaa aactagcaaa caccataaaa gactacaatt tttttatttt tataaacccc
     9841 tttaaatcaa cgtttctaag gtttttaaaa atctttttta atttattttc aaaaaacact
     9901 gtacattatg tcaatatgag cgtatagttg gtcttacgta ataaaagctc gtgaattaaa
     9961 ttgtagtgta tttgtttaga atatcctctt ttttagttat gaatttgtta caaatattaa
    10021 gtgcaaaagc acatggaggt tttctatatg aataacggta cagtaaaatg gtttaacgca
    10081 gaaaaaggtt ttggtttcat cgaacaagaa aatggcggag acgtattcgt acatttctca
    10141 ggtatcgcta gcgatggcta caaaacttta gaagaaggtc aaaaagttac tttcgaaatc
    10201 actgaaggtc aacgtggaga ccaagcagtt aacgtacaaa ctgtttaatc ttacaaaata
    10261 aaacgactca ttataaatga atcgctttga ttaccaacaa ggttctaaat cttgttggta
    10321 tttttttatg ctattttgga taatatgagg ccataccacg atgatttata aaaacttatt
    10381 tgccagatac acactaagga aaattatgag gctgagtata ccaattactg atataataga
    10441 aagcgcattt gtcatcgtat ttctagcttt agtcgtttgt acttcactag gatcttttcc
    10501 attcataaaa gcaacaattc gatcgtagtt gtccatttta tgcacttttt ttaaatcttc
    10561 tattttcata gatgcataaa aacctattcc caaaatgatt gccaccgtta caccccaagt
    10621 ccaattccaa taaacaacta aaggtcccat taaaattgca gctaatgtca tgaaaataag
    10681 catcacatat gtccataagt taagacttcg ttgcgcttta atatcaggta caaatggaat
    10741 ggtccctttt actaaatcat ctaaagttac attgaacaat tcgcacatca ttaataagtt
    10801 atgtatgtct ggtaagcttt tgtcattttc ccaattagaa atactctgcc tagatacata
    10861 taacttttca gcaagatatt cttgtgaata accatctcgt tccctatatt ttttactttg
    10921 tttactcaga ttcaattaca tacgcctcca gtcaattagt acaatcatta caatcagact
    10981 atcagataaa cttagttaaa gtcaccttta tgattgacta aatgttttga catacgacaa
    11041 catcagggtt tgaagacttc ctcaagacgt actcggaata tcttgacact tgtaattttc
    11101 taaaaaaatc tttcaatagt aattaataca tgcaaaataa atcctaacag tgatactacg
    11161 ttaacactat ttactattcg attttccagc tttccaacta tgcttttcta atcttttttg
    11221 tgctataaat tctactgctt caaaaataca tactgcactg atgaaccagc ttaggaaacc
    11281 aacaacaatg ctaatcaacc acatgtgcgt catatgtaac atcatccatg taactaaaat
    11341 acaaactaat acccctgtca taccaaacac tgcgccacga cttacatgca cagcgacttt
    11401 atcaccatat tgcatacctg taataaacat agacactaaa aatactgccg gaaacgttgc
    11461 aaatataccg ccaaattctt tccaaggtag tgttacagat acaatataac ttaataatac
    11521 tgcaaatccc cccacaaaaa atttcattaa tgttaatttc atgatgttct gcctccaaaa
    11581 atttattact taataactga atacaactaa ttatcacgca ttttccaaac tataaatgtc
    11641 taatactgaa tattgcgagg gaaataacaa accaacaaac aaccgtgaat attgcgcctt
    11701 tccgataacc tttatgctta atgtagagag atgtcaaaaa tacagttaat atacaagaca
    11761 gaataccgac aattgctcca gtactaagat tcatcgacat ctccactaat tgcgtaccac
    11821 gatgatctaa agctaatgcg ataatagccg caagaaagac tgccggcata gtagctataa
    11881 tacctcctaa cttaccacct accttatcag caataattga agctaatgca acagcgatac
    11941 caccaatgac aaaatgaaga attgcacttc caattgaaaa catcttcaac gtacctttct
    12001 ataattaatt caatacaaaa cgtatcattc acacaaagtg taaaacttaa attttctact
    12061 gtaaaggcaa ctcaagcgat gttcataaag ctttcatgat actttcataa actagcaata
    12121 aagggttcaa atgaaagtat atccagctca aattagggag tgggacagaa atgatatttt
    12181 cgcaaaattt atttcgtcgt cccacccc
//
LOCUS       DAHHYJ010000079        12187 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000079 DAHHYJ010000000
VERSION     DAHHYJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.079"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            383..1329
                     /gene="hlgC"
                     /locus_tag="NBK22_001658"
                     /pseudo
     CDS             383..1329
                     /gene="hlgC"
                     /locus_tag="NBK22_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
     gene            1331..2308
                     /gene="hlgB"
                     /locus_tag="NBK22_001659"
     CDS             1331..2308
                     /gene="hlgB"
                     /locus_tag="NBK22_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCD2414845.1"
                     /translation="MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKL
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEL
                     FLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLDTKETENNK"
     gene            complement(2360..2827)
                     /locus_tag="NBK22_001660"
     CDS             complement(2360..2827)
                     /locus_tag="NBK22_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCD2414846.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLIFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(2838..3530)
                     /locus_tag="NBK22_001661"
     CDS             complement(2838..3530)
                     /locus_tag="NBK22_001661"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCD2414847.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVQRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQIIEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDNRIDLNHYILFL
                     ESTPKQVVYETV"
     gene            complement(3541..4656)
                     /locus_tag="NBK22_001662"
     CDS             complement(3541..4656)
                     /locus_tag="NBK22_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCD2414848.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILN
                     SGYDANLALFNIFKNTNYVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLEK
                     YPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGV
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFT
                     SYLRYPTVTMPMLRISLSYFHTEQDIDRLFEILHQED"
     gene            complement(4634..5641)
                     /gene="bioB"
                     /locus_tag="NBK22_001663"
     CDS             complement(4634..5641)
                     /gene="bioB"
                     /locus_tag="NBK22_001663"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCD2414849.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEIDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            complement(5643..7001)
                     /gene="bioA"
                     /locus_tag="NBK22_001664"
     CDS             complement(5643..7001)
                     /gene="bioA"
                     /locus_tag="NBK22_001664"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCD2414850.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAATLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(6979..7665)
                     /gene="bioD"
                     /locus_tag="NBK22_001665"
     CDS             complement(6979..7665)
                     /gene="bioD"
                     /locus_tag="NBK22_001665"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCD2414851.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKITDQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIGVPIYEDTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELYTTT"
     gene            complement(7720..7887)
                     /locus_tag="NBK22_001666"
     CDS             complement(7720..7887)
                     /locus_tag="NBK22_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414852.1"
                     /translation="MQLLILTDIFYRIAVYICPNSLRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHTP"
     gene            complement(8118..9851)
                     /locus_tag="NBK22_001667"
     CDS             complement(8118..9851)
                     /locus_tag="NBK22_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414853.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLS
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIKNNILFGNPGATKEEIIRAAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFI
                     RIKSKSAGWKL"
     gene            complement(9876..11639)
                     /locus_tag="NBK22_001668"
     CDS             complement(9876..11639)
                     /locus_tag="NBK22_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414854.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGVVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSAAIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYSLTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIEKLPDGYDTNVGTVGDKLSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
BASE COUNT         4081 a         2165 c         1656 g         4285 t
ORIGIN      
        1 atttaagttg aaagcttaaa taaatattct caagttaata aataattaac ttttagatgg
       61 attcatcaaa aatcgtaaaa agaacaattt gtattttaca aacattaatt aaaaataaaa
      121 gcaagacatt cgtgcaatcg gttaccttaa attgtttaca actgtcaaca ataccaaggt
      181 tttattaact atatttctca caaaattagc ttttagcatt ccaaaaaaaa aggttaaatt
      241 gaacggaatt atggcatttt taacttaatt gtaaaaaaag ttgataatgg tcaattgtta
      301 atgaacagtt aattataata acgcccaaaa tatattatta tttaattaag ttaaataaaa
      361 ttatagaaag aaagtgaaac ttatgcttaa aaataaaata ttagctacaa ctttatctgt
      421 gagcttactt gcccctcttg ccaatccgtt attagaaaat gctaaagctg ctaacgatac
      481 tgaagacatc ggtaaaggaa gcgatataga aattatcaaa aggacagaag ataaaacaag
      541 taataaatgg ggcgtgactc aaaatattca atttgatttt gtgaaggata aaaaatataa
      601 caaagatgct ttgatattaa agatgcaagg attcattagc tctagaacaa catattacaa
      661 ctataaaaaa ctaatcatgt taaagctatg cgatggccat tccaatataa tattggttta
      721 aaaacaaatg ataaatatgt ttctttaatt aattatttac ctaaaaataa aattgaatct
      781 acaaacgtga gtcagacatt aggatacaat atcggtggta atttccaatc agccccatca
      841 ctcggtggta atggatcatt taactattct aaatcgatta gctatacaca acaaaattat
      901 gtaagtgaag tagaacaaca aaactcaaaa agtgttttat ggggcgtcaa agcgaattca
      961 ttcgccactg aatcaggtca aaaatcagcc tttgatagcg atttatttgt aggctacaaa
     1021 cctcatagta aagatcctag agattatttc gttccagaca gtgagttacc acctcttgta
     1081 caaagtggat ttaatccttc atttatcgcc acagtatctc atgaaaaagg ttcaagcgat
     1141 acaagcgaat ttgaaattac ttacggaaga aacatggatg tcactcatgc cattaaaaga
     1201 tcaacgcatt atggcaacag ttatttagac ggacatagag tccataatgc atttgtaaat
     1261 agaaactata ctgtgaaata cgaggtcaat tggaagactc atgaaatcaa ggtgaaagga
     1321 cagaattgat atgaaaatga ataaattagt caaatcatcc gttgctacat ctatggcatt
     1381 attattactt tctggtactg ctaatgctga aggtaaaata acaccagtca gcgtaaaaaa
     1441 agtcgatgac aaagttactt tatacaaaac aacagccaca gcagattctg ataaatttaa
     1501 aatttcacag attttaacat ttaatttcat caaagataaa agttatgata aagatacttt
     1561 agtacttaaa gctactggga atattaactc aggctttgtg aaacctaatc ctaatgacta
     1621 tgacttttca aaattatatt ggggagctaa atacaatgta tctataagct cacaatctaa
     1681 tgattcagta aacgtcgttg attatgcacc aaaaaatcaa aatgaagagt ttcaagttca
     1741 aaatacttta ggctatacat ttggtggtga cattagtatc tctaatggtt tatctggtgg
     1801 acttaatgga aatacagctt tttctgaaac aattaattat aaacaagaaa gttacagaac
     1861 aacattaagt cgcaacacaa attataaaaa tgttggctgg ggagttgaag cacataaaat
     1921 tatgaataat ggttggggac cttatggacg agatagcttc cacccaacat atggtaatga
     1981 actcttctta gctggcagac aaagcagtgc atacgctggc caaaacttca tagcgcaaca
     2041 ccaaatgcca ttattatcta gaagtaactt caatccagaa tttttaagcg tactatcaca
     2101 cagacaagat ggcgctaaaa aatctaaaat tacagtaact tatcaacgtg aaatggattt
     2161 ataccaaatt cgttggaatg gcttctactg ggcaggcgca aattataaaa actttaaaac
     2221 tagaacattt aaatcaacat atgaaattga ttgggaaaat cacaaagtga aattgttaga
     2281 tacaaaagaa actgaaaaca ataaatagct agtaaaacac ggtcgccaac agtaattgtg
     2341 acgaccgtgt tttgatttat tatcttagta agactgccat tctttttctc aatgtgagat
     2401 ataaaggaat agctacaatt aaagtgaata ttacgcctgg aatcgcgttt aacaacacta
     2461 cccacactgg taaatttaaa ataatagata atagtagcct agataccaaa ctgcctaata
     2521 cacttgctaa aactaatgat agtacattta ttttcaataa ataaacaact gcaatagcta
     2581 taactctaaa tataatagaa ataatcacat taattggatt aaatacgcca aatatcagca
     2641 ataataagct tgataaaagc ccacctaaaa agtacttttt aattccaaag aaagctaata
     2701 ttaataatgc tgccggtgca gataattgaa aatctaatcc tggtataatg gacggtattt
     2761 tcaaaactgc caaaatggtt aaaatcgcag caatgacact aatttgagta atatcttttg
     2821 atgtcatact aaaaccccta taccgtttca taaacaactt gcttcggtgt gctttctaaa
     2881 aataatatgt aatgatttaa atcaatacga ttgtccacaa atattattct gcctccatat
     2941 ctcgtattaa ctggtttaat atcaaacaat cgatggtaac caattttagc agaggcaaaa
     3001 taacctgtcg tatacgttaa gtcatcggac acgcaaagtt ctcctttgac atacggatgc
     3061 gcattgatac aactagcaat tgctaaggca tcagtcactc tttcattaag atcaccttta
     3121 ttatttatat cttcaaacga aaaatgtgtt gccctaatcc ccctttgtcc aaatgaatct
     3181 aaacgtttac cagatatagc agatagaata atagctcctg tataaaccgt ttcattttta
     3241 atatatgtca tcccttgatt tagcgcttgt tcagtgacac cacattcttg tgttaaatgt
     3301 tggagatttg ctttatcatc ctcaataatt tgtaatgctt ttatttgttg aatcggttcc
     3361 atgatttttt gtatttttat attgagaaaa tccggttgcc cattttcatg aaaaaaacct
     3421 ttattataaa acctttgtac cgtctgttct attttgtcaa actcacaaat cgtttcagca
     3481 ccactaatat gaacatcttg attgctagaa cgcattttta tactatacat gacgatcacc
     3541 tcaatcttct tgatgcaaaa tttcaaacaa cctatctata tcttgttcag tatgaaaata
     3601 cgacaatgat attcttaaca ttggcatagt cacagttgga taccttaaat aacttgtaaa
     3661 cacatgatgc tttaataatg tttgatgaat gttctcagcc gcttctatgt catcaaactc
     3721 aataaactta atcggcgagt ttgaactatt ataattaaca ttgagtgctt ttaacttttg
     3781 gttaaaatat ttactcaaac tatttaattt agtgcgtcta tcatcagcat ttattaactt
     3841 ttcaatgttt ctttttataa aatacaaatt ataaattggc aaactacttg agtagatgag
     3901 tggtctaccg tgattaatta acatatcctt cacatcattt gaacttaaaa tcacaccccc
     3961 gtatgcacca catgctttag ataaactaga agtgagtata tctacacctt gataattcga
     4021 gtaattctct attccaaaac tatgtgaaac atcgagtatc agtgttgcgt tatatttatg
     4081 ctttaatgag actaattgac caatatccac aacgtcgcca ttcgttgaaa atacactatc
     4141 agatatgatt atttttggta tattttgatt agggtatttc tctaaccttt tttctaaatc
     4201 agcaatatct aaatgcttat atatcacttt ttctaaacca cttaacttaa taccgtcaat
     4261 aatactcgca tgattttctt gatctgaaaa cacgacataa tttgtatttt tgaaaatatt
     4321 aaataacgcc aaattagcat cataaccact atttaagata gtacatgcac tatatccgag
     4381 ccaacctgct aacattgttt caatttcttc ataagctgtc gaacttccac taattaatct
     4441 tgaacttgat aagtgataac tatacttccg cataaatctt tcgaaatcat ccttatcaaa
     4501 cgctatttga cctaatccta aataatcatt agatgtatag ttcgtacatc tcttattttc
     4561 tacttcaata tactgtctat ctatataccc taccgattta agcgaccgat ataacccttt
     4621 ctgttgtaat aaatcaattt gctcttgaaa catcattctt gttttcctta ttttcacaag
     4681 tgtcataatc aatttcaaag cccaaatcat taatcatatc gtagtctaat tggttcggtt
     4741 gcccaccagt aattagataa tcaccgacaa atattgaatt cgccgctttt aatgctaatg
     4801 gctgtaacga acgtaagttg acctctcttc ctccagcaat acgaatttct ttcgtaggat
     4861 tgattaatcg gaataatgct acgattctta aacatttcat tggtgttaaa tcatccatgc
     4921 ttccaaactt tgtgcctttg attggatgca aaaaattaat tggaatactg tcggcatcga
     4981 tttctttcaa agcaaatgcc atatcaacaa tatcttgatt agattctccc ataccacaaa
     5041 tcacgccaga acatggtgat atgttattcg ctttcattag ttctatcgta tctgttcgat
     5101 ctttataact atgcgttgtc acgacgttat catggtaatt ttcacttgta ttaatattgt
     5161 ggttatatct gtctacacca gctgacttaa gtttcttagc ttgttcgtca ttcgttaatc
     5221 ctaaacatgc acagattttt agttgcgggt gttgagattt aatcgttctt acagtgttac
     5281 taatatgatc aacttcttta tcgctcggtc ctctaccact cataacaata caatatgttc
     5341 caatatgatt atcatgtgcc acctttgctc catcgataat ttgttcctct ggaattaaag
     5401 catatcgctg tttttgttta atatctcgtg attgtccaca gtacccacaa ttctcaggac
     5461 atataccact tttagcattt aaaatcatgt ttaattttac ttttttacca aaataatgtt
     5521 ttcttaaaat gtacgcctca tttaataaat ctaaggtatc aatattagta tcctcataaa
     5581 ttttcaatac agtctctttt gttaattgtt ccccttgtaa tatgcgttta gccaaattca
     5641 tattaacact tcctatctaa aatgttatgt actgcctttt taaaaatacg taccatcgtt
     5701 ttcacttctt tatttgacat acttaacact ggcacaaacg taatgacatt ttctaaattt
     5761 ctaatcatta gcccgttttc tttacattta cgtacaatac gttcaacttt ttcaatttct
     5821 aacggcgttt tactatcttt atctgtaact aattcaactc caaacattaa gcctcgtcct
     5881 cttacatcac caacatttcg atgacgcttc agcgcatgca actgtttctc aagtgttgat
     5941 gatgtcgttt caatatgtga caataactta cgtttttcat aaagtcttat attttctaat
     6001 gcaaccgtac aaacgatttg attcccggtg tatgtatgac catggaaaaa ggtattcaca
     6061 ccatgcgaat cacttaaaaa tgcattgtat attttttgag atgtcaatgt agctgcaagt
     6121 ggtaagtagc cacctgtaat cgccttacct aaacacataa tatccggttg aacatcttca
     6181 tgattgcatg caaacatctt tccagttctc ccaaaaccaa ctgctacctc atcacaaatt
     6241 aataagacat cgtatttttt gcacaatttc tcgacttctt tcaaaaagcc tttaggatga
     6301 acaaacaagc ctgtcgcacc ttgaatcaac ggttccaata taaaccctgc gatttcatca
     6361 tttctttcaa ttagaatttg ctctatattc gttaaaatag ctgtcatcat ttcattttca
     6421 gtatcgtaat tactgcgata gaaagatggg ctttctactt gaatattctc aaatattaag
     6481 tctttaaata ttttatgaaa ggtcttgata ccaccaacac ttaccgcacc aatcgtatcc
     6541 ccatgataac cgtgatttag cgttataaac ttgtttttct tggcatattt ttctctatca
     6601 atatttttcc aatactgata tgccatcttt attgcgattt caacagacgc actgcctgta
     6661 tcagaataaa atacttttct tagattactt ggcgtgattt cgattaattt ttccgcaagt
     6721 tctattgacg gaatatttga tgatcctagc agcgtagaat gggcaatttt attgagttgc
     6781 tttttaatta ccttatttaa gtatttgtta ttatgaccat gcacattgac ccacaacgat
     6841 gcataaccat ctaaatattt attgccattc gtatcgtaaa ggtaactacc ctttcctttt
     6901 tcaatgatga ttgcttcttc tttgctatat acacccattt gtgtaaatgg atgccaaaca
     6961 tattctgagt ctttttgttt aagttgttgt gtataattca tttttaactc ctattaattg
     7021 ttttaaaaat gcttctgaga aattttcata cgtggcatgt tcttcgaatg tatagactgt
     7081 tttatttgtt aattttccaa tcgtaatttg attgtctttt tcaatatagc tgtctgtata
     7141 gcgattcatt attaaaaaat tactcgccga tacattctga ttaacataat cttggtgaac
     7201 aatggcatcg ctaatagcac ctaattttga tggcaacaca ctgatgacac aatctgcaca
     7261 atcattgatt agatctttag tcatgtagaa atcatctgta tcttcatata ttggtacgcc
     7321 aattccccca gcaccctcaa ttaagataaa atcaaattcc ttatctaaaa cttttacctt
     7381 atctaataca cgctgcttat ttagaaaaat ttgatctgtt attttaaatg caaggtgtgg
     7441 tgatacaggt tgcttaaaag tataaagtga cgttatgtca tagcttaaat cacattcatt
     7501 tttaaatact tctaaatctg gaaacgtccc gtcttggcgt tcctcagttt gaaatggttt
     7561 aaaaatacaa actcgatgac cacgtgtttt caaagcatgg tataaatgct ttgtaacata
     7621 ggttttgcct acatcagtat tcgtacttgt aataaaaatc ctcataaaaa ataagcacct
     7681 caatccgaat gtaaactttt ataattaata agtttacatt taaggcgtat gtttttcaag
     7741 aaaacatgaa tttaaacttc tcagtataac tataaatttt ggcaaaattt tgaccttgct
     7801 attgaaatga ctaagacttc ttaaggaatt cgggcagata taaacagcga ttcgataaaa
     7861 tatgtcagtt aaaattaata attgcattat ttcttaataa aaggacatcc tagctttacc
     7921 atgttgtgaa acaatccatt aattgtaata ttacgttgag cgccttgttc atcaatgcta
     7981 taattagtta cataataaat tgaacatcta aatacaccaa atcccctcac tattgcccta
     8041 gtgaggggat ttattttgct gttgattatc tatcaccctt tttatttcca aattctgatt
     8101 ccatattaaa ctattattta taatttccag cctgctgatt tactttttat tcttataaag
     8161 tcttgataca ttcctggttt tcgaattaat tcatcatgac tacctttttg aattatttca
     8221 ccttcattga gcactataat ctgatctgca tttttaatag tttcaagttt atgtgcaatt
     8281 gtaattactg ttttgccttt acttaattca ttaattgccg tttgaatcag ctgttcattt
     8341 tcagggtcaa tacttgcagt tgcttcatct aaaataatta ttggtgcatc ttttaatatc
     8401 gccctagcaa tagaaatcct ttgcttttct ccgccagata aattactacc tttttcattt
     8461 agcattgttt gatatccctc aggtaatgac atgatgaagt cgtggcaaca tgcttgcttc
     8521 gctgcacgaa taatttcttc tttcgttgca cctggattgc caaacaatat gttattttta
     8581 atcgtatcat taaataaata caccttttga aatactgcac taattttcga cattaacgta
     8641 cttaatgtca tatctttaat atcaacacca tcgatgcgaa tatttccatc atcgatatca
     8701 taaaagcgca agagtaagtg acataatgta gatttcccac ttcctgaagg tccaattatt
     8761 gctgttgatg tttgtgtagg tatctcaaaa ttcacattct ttatcacttg tttatcatca
     8821 tatgaaaaat tcacgttttg aaaagcgata ttttcatttt taatcgtcaa attttttcca
     8881 ttctcatcca gttctggagc attttttatc ttttcaatat catctatcgt catatctatc
     8941 atttcaagta cgtgtgcagc actattaata ttttcaacac tatcaaatat cacaaatgaa
     9001 aaaattgaaa tcataataag ggtagataaa tcaatactat gattcatata taataagcat
     9061 gcaactaata caatcattat tgaaacaact tttaaactaa gtaaatgcaa taaattgaat
     9121 gggatatatt gcatttctat ttttgtattt atacgtttac tttcattgac tgcttggtta
     9181 aaacttttaa gactcgtatt ttctttcgcg aatgatttta ttacttgaat accacgaata
     9241 acctccaata ccttttccac taattggttt tgtacattat gatacgctgg cgcattttgt
     9301 cgactctttc tttctaataa ttgaatcgca aaaaatgata gtaatacgcc aatgcatgct
     9361 aataatgata cttgccacga aactacaagt agagacaata tgagtactgt aattaatatg
     9421 tatccattaa caacaacgtc caccattttc atagcaaagt tttctaaaaa ggttaaatct
     9481 gttgttacta ttgttgttaa ctcatttgaa tgatgcgaat taaaataacc taaccttaca
     9541 tttttcaatt tatcccctat atctaaacgt tctttcgcac tcatttcata agcgatgctc
     9601 tcatggcttt tgcttttgaa atatgctgta ataaatcgtc caatcactaa taaaaccatg
     9661 ataattacaa cattcaatat atctttcata taaataggtt tatgagatag cacattatta
     9721 aatatttttg cagctaaaaa gataggtaac gcaataaaaa tagcatttaa aaatgacatg
     9781 ctaaatccta aaatcattct tgctttatat ggtcttatcc agtttaaaat tttaaatgta
     9841 atttgaaaca tatttgacct ccttttctca ttagtttaat tatgtccagt attgattccc
     9901 caatcttttg tatgcatgtg cgtatcccac attttcttat aattaccgtt taattttagc
     9961 aataagtgat gtgacccttt ttctaaaatt tgttgtttac ctaaaacaat aatttgatct
    10021 gcatgttgaa tcgtagataa ccgatgtgca ataacaatca atgttttatc ctgcgtcaac
    10081 acatttaatg cttcttgaat tttttgttcg ttgtctggat caacgtaagc agtcgcttca
    10141 tctaatacaa tgataggcgc atcttttaat atcattcttg caatagtgac tcgttgtttt
    10201 tcaccaccag acaatttatc tcccactgta ccaacattcg tatcatatcc atctggcaac
    10261 ttttcaataa attcatgaca ttgtgctaac ttggcagctt tttcaactgc ctcatccgta
    10321 gcttctggat tgccaagttt aatattttct ttaaaagtaa gatttaataa aaagttatct
    10381 tgtccaacaa aaccaactaa atcgttaagt tgtttcgatt caatatcttt tatatttata
    10441 ccgccaatcg taatttcacc tgaagtcaca tcccagtatc gtgatataag cttggcaatg
    10501 gttgacttac cgctaccaga tgccccgacg atagctgtga aattattttc tggtactgta
    10561 aatgataaat gcttaaagac cagatcgtcc ttatctttgt tatatgaaaa tccaacatta
    10621 ttaaaagcaa tttcataatg ttgaggcttc ttaaattttg ttgacaatac taactcttct
    10681 agacttaata tttgattcac ttcagttaat gagtattgta tagactttaa atgatttaca
    10741 taattagtaa aattcttaat cggtgctact acacctaaag ataatacgat gcataggaaa
    10801 aattccgcat agtttaattg gttgatagat atcaaataca tgccgaccgg taaaatccct
    10861 aaaaatgttg aaggtaatac actagctcct aaattcatat acccccatgt atttttaaac
    10921 caattcaaag tgtgaatctt ataattatct actgcatctt tatatttttt atatgaactt
    10981 tgagattgat taaatgtttt aatcacttca atgccttcga taaactctac aatcgcgcta
    11041 ttcatataat tattcgattt catttgttca gcatatgtct cattaaatcc agacataact
    11101 tttttaaaag cgaaaattga aattggtatc gttactaata aggcactagc catacgccaa
    11161 tcaatgagca ttatgtataa aaagatagca gctgacaaaa gtaagtttcc tataacttca
    11221 ggaatcatat gtgctaaagg taattctatt gtttcaacct tatcgacaaa tatatttttt
    11281 aattcaccta ttttcttaga ttccactacg cctaaaggga gacgcattaa tttttgagct
    11341 aattttttac gaatttcaga taaaatttca tatgccgtaa tatgtgatag catcgttgac
    11401 gctccaaaac aacacacttg tgaaatataa gcgattaaag caataaagat ataaaccata
    11461 atcgaattaa tcgtatatgc attgttaatc atcattaaaa taattttaaa gactgcccaa
    11521 tatggaacta atccagaaaa gacactgatg atagacaaca aaattgataa cataattttc
    11581 catttactat cagaagcaaa tccgctgagt atctttatcc aatttgtctg attgttcatc
    11641 gtatatgtaa ctcctttcaa ttaataatct aaattaagcc gcttatatta tttatttcac
    11701 tggatgatat acataatata aatttgttat ttgttaaaaa ttaatactta ttacaagtac
    11761 atcatatatt agttgataac gattatcaat gtcgcgtgga tttgtgacac atttctttta
    11821 aaaattcaca aggttatggg gcagaaatga taaagagcca ctaatgattt attatgtagt
    11881 ggttctggga gtgggacaga aatgatattt tcacaaaatt tatttcgtcg tcccacccca
    11941 acttgcattg tctgtagaaa ctgtggatcc aatttctcta tgttgggtcc ctgagtatag
    12001 ccttgtagag tctagtacat tgatttgtat cccaatgtcc ctataattga ttattcgctt
    12061 tatctaatga tcctatgact caactattaa atcatttttc gaaatactta attctaatat
    12121 aattaaattc atttattgta atattgcaaa aatacattgc acaccttgtt catcaatgct
    12181 ataatta
//
LOCUS       DAHHYJ010000080        12166 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000080 DAHHYJ010000000
VERSION     DAHHYJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.080"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            366..752
                     /locus_tag="NBK22_001669"
     CDS             366..752
                     /locus_tag="NBK22_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCD2414855.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            745..1041
                     /locus_tag="NBK22_001670"
     CDS             745..1041
                     /locus_tag="NBK22_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD2414856.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      1099..1202
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1291..2316
                     /locus_tag="NBK22_001671"
     CDS             1291..2316
                     /locus_tag="NBK22_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCD2414857.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLSKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            2309..3004
                     /locus_tag="NBK22_001672"
     CDS             2309..3004
                     /locus_tag="NBK22_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414858.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            3022..3843
                     /locus_tag="NBK22_001673"
     CDS             3022..3843
                     /locus_tag="NBK22_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD2414859.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGHQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            4197..4391
                     /locus_tag="NBK22_001674"
     CDS             4197..4391
                     /locus_tag="NBK22_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD2414860.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(4624..5475)
                     /locus_tag="NBK22_001675"
     CDS             complement(4624..5475)
                     /locus_tag="NBK22_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCD2414861.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTSLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFAGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
     gene            5803..6564
                     /gene="sufC"
                     /locus_tag="NBK22_001676"
     CDS             5803..6564
                     /gene="sufC"
                     /locus_tag="NBK22_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCD2414862.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            6662..7969
                     /gene="sufD"
                     /locus_tag="NBK22_001677"
     CDS             6662..7969
                     /gene="sufD"
                     /locus_tag="NBK22_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCD2414863.1"
                     /translation="MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRVTALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDKNASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            8075..9325
                     /locus_tag="NBK22_001678"
     CDS             8075..9325
                     /locus_tag="NBK22_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD2414864.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTTDGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDIDQLINALKQTKEFFSYEF"
     gene            9315..9779
                     /locus_tag="NBK22_001679"
     CDS             9315..9779
                     /locus_tag="NBK22_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCD2414865.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            9930..11327
                     /gene="sufB"
                     /locus_tag="NBK22_001680"
     CDS             9930..11327
                     /gene="sufB"
                     /locus_tag="NBK22_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCD2414866.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENIVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            11514..11653
                     /locus_tag="NBK22_001681"
                     /pseudo
     CDS             11514..11653
                     /locus_tag="NBK22_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11659..11973)
                     /locus_tag="NBK22_001682"
     CDS             complement(11659..11973)
                     /locus_tag="NBK22_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414867.1"
                     /translation="MYFVLAIFTIISASVSLGYSIQACASSHNINAYYALSRSLPLFL
                     LAIFSLVIHSAIFLITISIAMILVQFLDAIVGYKSEDVFKTYGPLATSVVNLILLIVF
                     LF"
BASE COUNT         4413 a         1713 c         2247 g         3793 t
ORIGIN      
        1 ccaacttgca cattattgta agctgacttt ccgccagctt ctgtgttggg gccccgccaa
       61 cttgcacatt attgtaagct gacttaccat cagcttctgt gttggggccc cgctataatt
      121 gaaaagtttg ttataggtgt attttctttt ggttaactat tgttaatata acattgtaga
      181 ttttaggatg ttgatttttg acttagcctt tgtctgctta tgttaaatgt tccagctagt
      241 caaacattga gtttgtaatt attatattaa gctagtttaa attttttgtg atttatgttt
      301 tattcatacc aattttgtga aatgatacag tgacagatgt ttattgattt taaagggtga
      361 tgatgatggc tattgtaaat aaagtgataa ttgttgaagg aaaatctgat aaaaaaaggg
      421 tgcaacaggt tattgcagaa ccagtcaata ttatttgtac tcatggaaca atgagtatag
      481 ataagcttga tgatatgata gaatcactgt atgataaaca agtttttgta ttagccgatt
      541 ctgatgacga aggagatcga attagaaatt ggtttaaacg ttatttgagt gaaagtgaac
      601 atatatttat tgataaaact tactgtcaag ttgcgaattg ccccaaacaa tatttggcgc
      661 atgtactttc aaaacatggc tttacttgta agaaagaaac acctctttta ccgaatataa
      721 ataatgaaag gttagtttta gtaaatgaat aattcattag acatcaaaga tgtaactaca
      781 ttttatgagg aagacaaaca tttaatcttt ggttatacac caacgtgtgg tacttgtaag
      841 gtttcagaaa gaatgttaga cattgctaat gaaatattgc agttaccatt attgaaaata
      901 gatttaaact tttatcctca gttttgtaaa gatatgcaaa tcatgtctac gccgatttta
      961 ttgttgatga ataaagataa agaagtaaaa cgaatttatg catttaaatc ggtgactgat
     1021 ttgttagaaa atttaaaata gttattgacg aaattttact aaggtgttag gattaataca
     1081 aattaaatga tgcgtaaact cttatcgaga gtggtggagg gatgtgccct acgaagcccg
     1141 gcaaccgtct tatatagaaa tggtgccaat tcacataaag ttttaacttt tgaagatgag
     1201 agaaacaata ctactattgc tttctcaatt tttctatcga tattgagaaa gcatttttta
     1261 ttttattaag caacacaggg aggaatcaac gtgattgaat taaaagaagt tgttaaagaa
     1321 tatcggacta aaaataaaga agtccttgct gtagatcacg ttaatttatc gattcgagca
     1381 ggatcgattt atggcgtcat tggtttttct ggagcaggaa aaagtacttt gattcgaatg
     1441 tttaatcatt tagaagcgcc tacatcaggt gaagttatta tagatggaga ccatataggt
     1501 caattgtcca aaaatggatt aagagcaaaa agacaaaaag taagtatgat cttccaacat
     1561 tttaatttgt tatggtcaag gactgtgtta aaaaatatta tgtttccgct tgaaattgca
     1621 ggtgtcccta gaaggagagc taagcaaaaa gcattagaac ttgtcgaact cgtcggttta
     1681 aaaggtagag aaaaggctta tccatcagag ttatcaggtg gacaaaagca acgtgttggg
     1741 attgcacgag cgttagctaa tgatccaacg gtcttgcttt gtgatgaggc aacaagtgca
     1801 cttgatccgc aaacaacaga tgaaatttta gatctactac taaaaattag agaacaacaa
     1861 aatttaacaa ttgtactaat tacgcatgaa atgcatgtca ttcgtcgtat ttgtgatgaa
     1921 gttgcagtta tggaaagtgg taaagtgata gaacaaggac cggtgacaca ggtttttgaa
     1981 aatccgcaac acactgtgac aaaacgattt gtgaaagacg atttaaatga tgatttcgaa
     2041 acatctttaa cagaattaga gccattagaa aaagatgcat atatcgttag attagttttc
     2101 gctggttcaa caacaaccga gcctattgta tcgagtctat caactgccta tgatattaaa
     2161 attaatattt tagaagcaaa tattaaaaat acaaaaaatg gaacagtcgg ctttttagtt
     2221 ctgcatattc catatatttc aagtgtagat ttcggaaaat tcgaaaaaga gttaattgag
     2281 cgacaagtta aaatggaggt gttaagacat gggtaaatca tttagtgaaa ttataaatga
     2341 aatgattaca atgcctaata ttcagtggcc agaagtttgg actgcaatag tcgaaacact
     2401 atacatgaca gtcgtctcaa ctatatttgc atttatactt ggtcttattt taggtgtgtt
     2461 attattcttg tctgctaaag gtaagtctat cggtgcaagg ttattttatt ctatcgtttc
     2521 tttcattgtt aacttattta gagcgatacc atttattatt ttaattttat tattaattcc
     2581 atttacaagt ttgatacttg gaacgataag tggtccgaca ggtgcgttac cagccttgat
     2641 cattggcgca gcaccgtttt atgcaaggct cgtagaaatt gcttttaaag aaattgataa
     2701 aggtgtcatc gaagcggctt ggtcaatggg cgctaatact tggacagtaa ttcgtaaagt
     2761 ccttttacct gaagctatgc cagcgctagt gtctggcatt acagttacag caatcgcttt
     2821 agttggttca acagcagttg caggtgtaat tggtgccggt ggtttaggaa atttagcata
     2881 cttaacaggt ttcactcgaa atcaaaatga tgtcatttta gtatcaacag tttttatttt
     2941 aattattgta tttataatcc aattcattgg ggattggctt acaaataaac ttgataaacg
     3001 ataaattggg ggtttcattt tatgaaaaaa ttatttggtc ttattttagt attaacattt
     3061 gcagttgtat tagcagcttg cggtaatgga aacaaaagtg gcagtgacga caagaaaata
     3121 acagtaggtg cttcaccagc accacatgct gaaattttag aaaaagcaaa accattatta
     3181 gagaaaaaag gttatgaact agatattaaa acaattaacg attacactac acctaataaa
     3241 ttactagaca aaggtgaaat tgacgcaaac tatttccaac atacaccata tttaaacaca
     3301 gagaaaaagg ataaaggtta caaaatcgta agtgccggtg atgttcactt agaacctatg
     3361 gctgtgtact ctaaaaagta taaaagttta aaagaattac caaaaggtgc aacagtctat
     3421 gtgtctaata atccagctga acaaggacgt ttcttaaaat tcttcgttga tgcaggttta
     3481 attaaaatca aaaaaggcgt aaaaattgaa gatgctaagt ttagtgatat tacagagaat
     3541 aaaaaagata ttaagtttaa taataaacaa tcagcagaat tcttacctaa aatttatcaa
     3601 aatgaagacg ctgatgctgt tatcattaat tcgaactttg caatcgaaca aaaactaaat
     3661 cctaaaaaag attctattgc tgtagaaagt gcgaaagata atccttatgc aaacttaatt
     3721 gctgttaaag aaggacatca agatgataag aaaatcaaag cattaattga agtattacaa
     3781 tctaaagata ttcaagactt cattaatgaa aaatacaatg gtgcagttat tcctgctaaa
     3841 taaatcatat taatggctca gtaacgtgat gttgctgggc tttttaattt aaacaggtat
     3901 ttatatgata tttaggaatg agatgctatt acggataaag taaaatccta tatgcatgcg
     3961 tgctactcat ttattcttat ttttaatttc acatggctag tgactaatac atcataaatc
     4021 attagtgatt ctttattatt tcagtcccac tccctcaagt taatgatgaa tctatttcgc
     4081 tagttataaa atgacgtcaa atcaatatca aaaattattt aagtaaaatg tttagataat
     4141 ttttcagtgg gtaagtatta tatataacat ttaattattc cgaggaggca tttattatgg
     4201 cagacgaaag taaatttgaa caagcaaaag gtaatgttaa agaaacagta ggtaatgtta
     4261 ctgataataa aaatttagaa aacgaaggta aagaagataa agcttctggt aaagcgaaag
     4321 aattcgttga aaatgcaaaa gaaaaagcaa ctgattttat tgataaagta aaaggtaaca
     4381 aaggcgagta attttacttt ttattcctaa tacactgcac atgccactta gtcggtataa
     4441 ccatcggcta ggtggttttt gtattaaaaa agtggatacc aaaatttatt aataattatt
     4501 ttaatgttag aaaaaaacta aataaaaact cgctaatgat atccaataat atgtatacaa
     4561 aacgagacat atattgcata tgattaacga gatactgaaa atattttatc acccctaaaa
     4621 tgattattca ttttcagcgg taattcgacc taaagtcaaa cttacaataa aaccgatgat
     4681 aaatactact aatgaaacga accacatcac gatattagtt ggtaaacctg gaaatactgc
     4741 aaagagggag ccaacaacaa aaccaatgat taatgcaaaa gtcattagtt tatgatgtgt
     4801 taggaaatac tggataattt tgcttgaaat aatgaatcca gcaagcacgc caaatccgac
     4861 tgcaagtaat ataggaagac ctgcaaagtt aagtttaaca acttcagata ttgctagcat
     4921 gaccgtacca tagacgccaa atactaataa cataaatgac cctgaaatac ctgggagtaa
     4981 catagcacta gatgcacaca tacctgcaat aaaatattta ataataagac tagttgatag
     5041 agtaagtgtt tctccagcat gtttatcacc attattcatt aatgtaataa caattaagat
     5101 agcgatacca gctataacca tcatgtaatg tttagttgta aatgacgttt tatagttaga
     5161 aattttcaat aaatatggaa cgataccaat gattaatcca ccaaagaaaa acatagttgg
     5221 aatatggtgt tggcttaata aataattaaa aagattactt agtgatccca ttgccagtaa
     5281 cattccaatt ataatgggga ttaaaaatgt aaaacttggc caaaaacgtc gtgagaatat
     5341 gccgctaatt gaagcgataa attgattgta aatacctaac aataatgcga tagtcccacc
     5401 gctaacacca ggtaccaagt cactcgttcc catagcaaaa ccttttagaa tattaatcca
     5461 tttaaattgt tgcatgaata actcctttca aacgattgga ataaaatcat aaatagcatc
     5521 ataccatatt acaaatgtcc tagtgaaatg ataacatatt ttaaattcat aaaatccatt
     5581 gagaaattat gtgcacttat tatcatttat atttttaaag agagcggatt agagttgtac
     5641 tttagagtta ttaaaaataa taaaaagggt ttaagttgtt tatattaagg tataagtaag
     5701 ttataattaa ctgaacgcat tattacaaag tctttttgac tacaaattaa aattattata
     5761 aactagttaa gaaaacttta tattttacgg agggaatata aaatggcatc aacattagaa
     5821 atcaaagacc tacatgtgtc tattgaggat aaagaaatct taaaaggtgt taacttgaca
     5881 attaacactg atgaaataca tgcgattatg ggaccaaacg ggacaggtaa atcaacttta
     5941 tcatctgcaa ttatgggaca cccaagctat gaagtaacta aaggagaagt acttttagac
     6001 ggtgtaaata ttttagaatt agaagttgat gaaagagcaa aagcaggatt attcttggca
     6061 atgcaatatc catcagaaat tacaggtgtt acaaatgctg atttcatgcg ttcagcaatc
     6121 aatgcgaaac gtgaagaagg acaagaaatc aacttaatgc aatttattaa gaaattagat
     6181 aaaaacatgg attttctaga catagataaa gacatggcac aacgttattt aaatgaaggt
     6241 ttctcaggtg gagagaagaa acgtaacgaa atcttacaat taatgatgtt agaacctaag
     6301 tttgcaatct tagatgaaat cgattcaggg ttagacatcg atgcattaaa agttgtatct
     6361 aaaggtatta accaaatgcg tggggaaaac tttggtgcat taatgattac acactatcaa
     6421 cgattattaa attacattac tcctgataaa gtacatgtaa tgtatgctgg taaagtcgtt
     6481 aaatctggtg gtccagaatt agcaaaacgt cttgaagaag aaggatatga atgggttaaa
     6541 gaagagttcg gttcagctga ataatcttat taatacagta tccatgagat gttcatctat
     6601 atatgatgaa aatgaacatt tatacgaaat agtaaatttc atcaagtagg aggaaaaagt
     6661 tatgacaact gatattttga acatttctga agaacaactt gttgattatt ctaaagccca
     6721 caatgaacct tcttggatga cagaattacg taaaaaagct ttgaaattaa cagaaacttt
     6781 agaaatgcca aaacctgata aaacaaaatt aagaaaatgg gattttgatt cttttaaaca
     6841 acacgatgta aaaggtgatg tttatcaatc tttatcacaa ttacctgagt cagtaagaga
     6901 aattattgac gtagatcatt ctaaaaactt agtaattcaa cataataata cgattgcgta
     6961 cacacaagtt gatgataatg catctaaaga tggcgttatc gttgaaggtt tagcagacgc
     7021 tcttatgaac catagtgatt tagtacaaaa gtactttatg aaagatgcag taacagtaga
     7081 tgaacatcgt gtcacagcgc tacacacggc attagttaat ggtggcgtat ttgtttatgt
     7141 tcctaaaaat gtagttgtag aacatccagt acaatacgtt gtgttgcacg acgacaaaaa
     7201 tgcaagcttt tataaccatg ttatcatcgt tactgaagaa agcgccgaag tcacatatgt
     7261 tgaaaattac ttatcaaatg catctggtga aggaaatcaa ttaaatatta tttctgaagt
     7321 gattgctggt gcaaattcaa atatcacata tggctcagtg gactatatgg ataaaggctt
     7381 tacaggtcat atcattcgac gtggtattac tgaagcggat gcctcaatta attgggcact
     7441 aggtttaatg aatgagggta gccaaattat tgataataca acaaatttat ttggtgatcg
     7501 ttcaacaagt tcacttaaat cagtagttgt aggtacaggc gaacaaaaaa tcaatctaac
     7561 atctaaaatc gtacaatatg gtaaagaaac agatggttat atccttaaac atggtgttat
     7621 gaaagaacat gcatcatctg tatttaatgg tatcggctac attaagcatg gtggaactaa
     7681 atcaattgct aatcaggaat cacgtgtatt aatgttatct gaacatgctc gtggtgacgc
     7741 gaatcctatt ttattaattg atgaagatga tgtacaagct ggtcatgctg catcagtagg
     7801 tcgtgttgat ccagatcaac tttactattt aatgagtcgt ggtatttctc aaagagaagc
     7861 ggaacgtctt gttatacatg gtttcttaga tccagtagta cgtgaattac ctatcgaaga
     7921 cgttaaacgt caattgagag aagtaattga acgcaaagtt tctaaataat attttgaaaa
     7981 taaaagtttg taatagatat agactgtcga tattggtata agactaatac aacgtcagta
     8041 tttaaatgat taggattttt atttaagaaa ggtcgtgaat gaagtggccg aacactcatt
     8101 tgacgttaat gaagtaatca aggattttcc gatattagat caaaaagtca atggcaaacg
     8161 tttagcatat cttgattcaa cagcgacaag tcaaacgcct gtgcaagtgt taaatgtttt
     8221 agaggattac tacaagcgtt ataattcaaa cgttcatcgt ggtgttcata cattaggatc
     8281 attggcaact gatggttatg aaaatgcccg tgaaaccgtt cgtcgtttta ttaatgcgaa
     8341 gtattttgaa gaaatcattt tcacacgcgg aacaactgca tcgattaacc ttgtagcaca
     8401 tagctatggt gatgcaaatg ttgaagaggg cgatgaaatt gttgtcactg aaatggagca
     8461 tcatgccaat attgttcctt ggcaacagtt agcaaagcgt aaaaatgcga cattgaaatt
     8521 tataccaatg acgactgacg gtgaattaaa catcgaagat attaagcaaa cgattaatga
     8581 taaaacaaaa atcgttgcta ttgcacatat ttctaatgtg ctcggtacaa ttaatgatgt
     8641 taaaaccatt gcagaaatag ctcatcaaca tggtgcaatt atcagtgttg atggggcgca
     8701 agcagcacca catatgaaac ttgatatgca agaaatgaat gctgattttt atagttttag
     8761 tggtcacaaa atgcttggac caacaggtat tggcgtatta tttggtaaac gtgagttact
     8821 acaaaaaatg gaaccgattg agttcggtgg cgacatgatt gattttgtaa gtaagtatga
     8881 tgcaacatgg gctgatttac ctactaaatt tgaggcgggt actccattaa ttgcacaagc
     8941 aattgggctt gcagaagcta ttcgctattt agaacgcata ggttttgatg caattcataa
     9001 atatgaacaa gaattaacga tatatgctta tgagcaaatg tctgcaattg aaggaattga
     9061 aatttatggc ccgcctaagg atcgtcgtgc aggtgtaata acgtttaatt tacaagatgt
     9121 acatccacac gatgttgcta cagccgtaga tacagaaggt gtagcggtta gagctgggca
     9181 tcattgtgcg caaccgttaa tgaaatggtt aaatgtgtct tcaacagcta gagcgagttt
     9241 ttatatatac aacacgaaag aagacattga tcagttaata aatgccttga aacaaacgaa
     9301 ggagtttttc tcttatgaat tttaataatc tagatcaatt atatagatct gtcattatgg
     9361 atcattataa aaatcctaga aataaaggtg tattagataa cgggtctatg acagtagata
     9421 tgaataaccc gacatgcggt gaccgtatac gactaacatt tgatatagaa gacggcatta
     9481 taaaagatgc taagtttgaa ggtgaaggtt gttcgatttc aatggcaagt gcatcgatga
     9541 tgacacaagc tgttaaaggt cattcacttg gagaagcaat gcaaatgagc caagaattta
     9601 cgaaaatgat gcttggtgaa gactatgtga ttacagaaga aatgggagat attgaagcat
     9661 tgcaaggtgt atctcaattc ccagctcgta ttaaatgtgc cacattagct tggaaagcat
     9721 tggaaaaagg tactgttgct aaagaaggta aagcagaagg tacgactgaa gaagaataga
     9781 atgctgttaa tcatagatga ttttgatatt agacatataa aagtataaaa atttttataa
     9841 gatgtcatgt cattgttata atatggttta catcatgaat taaaaactta cgcacgccgt
     9901 tgtaaatata tttttaagga gtgattgaaa tggctaaaaa agcacctgat gttggggatt
     9961 ataaatatgg attccacgac gatgatgtgt ccattttcag atcagaacgt ggtttaactg
    10021 agaatatcgt tagagaaatt tctaacatga aaaatgagcc ggaatggatg ttagatttcc
    10081 gtcttaaatc attaaaattg ttttataaaa tgccaatgcc tcaatggggt ggcgacttat
    10141 cagaattgaa tttcgatgac attacttact atgtaaagcc ttcagaacaa gctgaacgtt
    10201 catgggatga agtgccagaa gaaattaaaa gaactttcga taaattagga attcctgaag
    10261 ctgaacaaaa atatttagct ggtgtttctg ctcaatatga atctgaagtt gtttaccata
    10321 atatggaaaa agaacttgaa gaaaaaggta ttatctttaa agatacagat agtgctttac
    10381 aagaaaatga agaattattc aaaaaatact ttgcttctgt agtacctgca gcagataaca
    10441 aatttgcggc gttaaactca gcagtatggt caggtggttc gttcatttat gtacctaaaa
    10501 atatcaaact agatacgcca ctacaagctt atttccgtat taactctgag aacatgggtc
    10561 aatttgaacg tacattaatc attgctgatg aaggtgcttc tgtacattac gtagaaggtt
    10621 gtactgcacc agtttataca actagttctt tacactctgc tgttgtggaa atcattgtgc
    10681 ataaagatgc gcacgttcgt tatactacga ttcaaaactg ggcgaacaat gtatacaatt
    10741 tagttacaaa acgtactttt gtttatgaaa acggaaatat ggaatgggta gatggtaact
    10801 taggttctaa gttaacgatg aaatatccaa actgtgttct tttaggcgaa ggtgcaaaag
    10861 gtagtacatt atctattgca tttgctggta aaggacaagt tcaagatgcc ggtgctaaaa
    10921 tgattcataa agcgccaaat acatcttcaa cgattgtttc taaatctatt tctaaaaatg
    10981 gtggtaaagt tatttatcgc ggtattgttc attttggacg taaagcaaaa ggtgctcgtt
    11041 caaatattga atgtgataca ttaatcttag ataacgaatc aacatcagat acaattccat
    11101 ataacgaagt attcaacgat caaatatcat tagaacatga agccaaggtt tcaaaagttt
    11161 ctgaagaaca attattctat ctaatgagtc gtggtatttc tgaagaagaa gcgacagaaa
    11221 tgattgttat gggattcatc gaaccattta caaaagaact tccaatggaa tacgcggtcg
    11281 aaatgaaccg tttaatcaag ttcgaaatgg aaggtagtat tggatagctt taaaccgcgt
    11341 tgttaagcta ttcttgactt ccggaaatgg gtattgatac cattttgata ctgaatataa
    11401 caaaaagcca cattactgtg gctttttttg ttttataact aaatcggatt gatagataag
    11461 ctttgtactt atttatatca gtccgatttt ttgattggtg taaaaaataa tcattgatgg
    11521 tggataaagc gacaacacaa atacaatatg attgtggcat tagagtgctg gtctttatta
    11581 aattaattga aaactacatc aaatattctt taaagataat tcgatattag ttcgattaag
    11641 attcgttgta taagtgagtt aaaataagaa aactattaat aatattaagt tcacaacaga
    11701 tgttgctaat ggaccataag ttttaaagac atcttcactt ttataaccaa caatcgcatc
    11761 taaaaattga actaaaatca ttgcaatgga tatagttatc aaaaatatag cactatgaat
    11821 gactaaagaa aaaatagcta ataaaaataa aggtaagctt cgactaagtg cataatatgc
    11881 atttatatta tggctagatg cacatgcttg aattgaataa cctaaactta cactggcact
    11941 gattattgta aatattgcta aaacaaaata catgttaatc catctttcta tatttggata
    12001 taaacaagta cttgtctaaa gttatttaaa agataattag aataaattta tgagaaactt
    12061 attgttatca ttataatggt ttcaattcat tataactatg tcataaactg aatttgttaa
    12121 aatttttcat tatgtaaatt tattaataac aaacagctcg aactat
//
LOCUS       DAHHYJ010000081        12077 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000081 DAHHYJ010000000
VERSION     DAHHYJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12077
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.081"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..139
                     /locus_tag="NBK22_001683"
     CDS             <1..139
                     /locus_tag="NBK22_001683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414868.1"
                     /translation="WGEGPNTEADEKSAYNNVQVGDGAQQREIGFPISTDNASWGGTT
                     K"
     gene            475..3180
                     /gene="acnA"
                     /locus_tag="NBK22_001684"
     CDS             475..3180
                     /gene="acnA"
                     /locus_tag="NBK22_001684"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCD2414869.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            3360..3827
                     /locus_tag="NBK22_001685"
     CDS             3360..3827
                     /locus_tag="NBK22_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD2414870.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDGIDSL"
     gene            complement(4034..4330)
                     /locus_tag="NBK22_001686"
     CDS             complement(4034..4330)
                     /locus_tag="NBK22_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCD2414871.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(4706..5314)
                     /gene="plsY"
                     /locus_tag="NBK22_001687"
     CDS             complement(4706..5314)
                     /gene="plsY"
                     /locus_tag="NBK22_001687"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCD2414872.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLFAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFIVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            5520..7511
                     /gene="parE"
                     /locus_tag="NBK22_001688"
     CDS             5520..7511
                     /gene="parE"
                     /locus_tag="NBK22_001688"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCD2414873.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     YHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKTAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            7511..9913
                     /gene="parC"
                     /locus_tag="NBK22_001689"
     CDS             7511..9913
                     /gene="parC"
                     /locus_tag="NBK22_001689"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCD2414874.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDSSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKENLIEVFEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFEKDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            10163..11623
                     /locus_tag="NBK22_001690"
     CDS             10163..11623
                     /locus_tag="NBK22_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCD2414875.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
BASE COUNT         4224 a         1731 c         2305 g         3817 t
ORIGIN      
        1 ttggggtgag ggccccaaca cagaagctga cgaaaagtca gcttacaata atgtgcaagt
       61 tggggatgga gcccaacaaa gagaaattgg attcccaatt tctacagaca atgcaagttg
      121 gggtgggacg acgaaataaa ttttgcgaaa atatcatttc tgtcccactc ccatcaaaag
      181 aatgacattg aaaagaaata ttaaagtttg gtgccataat tttctttctg ctcaatgaaa
      241 taatacgttt gaaaattgaa ttgaatcatt cacgaaattg ataattattc tcaattaata
      301 aaaaatactt attacacctt gcccgcatga taagtttttc ttatatactc tgattaaaaa
      361 gtcaaaacct aaattaagcg ttttctaagc atgatgagtt gtttatcaat aaatataata
      421 atacaattta atcaaggcat ataaatataa aaatgtatca agggggatca ttaaatggct
      481 gcaaatttta aagagcaatc aaaaaaacat tttgacttga atggccaaag ttatacttac
      541 tatgatttaa aagctgtaga agagcaaggt attactaaag tttccaattt accttattca
      601 attcgtgttt tgttagaatc tttacttcgt caagaagatg attttgtaat tacagacgat
      661 catattaaag ctttaagtca gtttggaaaa gatggaaatg aaggcgaggt accatttaaa
      721 ccttctcgtg ttattttaca agatttcaca ggtgtaccag ccgtagttga tttagcttct
      781 ttacgtaaag caatggatga cgttggggga gatattacta aaattaatcc agaagtaccg
      841 gtggatttag ttattgacca ctcagttcaa gtggatagct atgcaaatcc agaagctctt
      901 gaacgtaata tgaaattaga atttgaacgt aactatgaac gttatcagtt tttaaattgg
      961 gcaacgaaag catttgataa ttacaatgca gttcctcctg caactggaat agttcaccaa
     1021 gttaacttag aatatttagc aagtgttgta catgttcgtg atgtagatgg tgaaaaaact
     1081 gcatttccgg atacattagt tggtactgat tcacatacaa caatgataaa tggtattggc
     1141 gtactaggat ggggtgttgg tggtattgaa gctgaagctg gaatgcttgg acaaccttct
     1201 tatttcccaa ttccagaggt tattggtgta cgactagtaa attcattacc acaaggcgca
     1261 acagcaactg atttagcgtt aagagtaact caagagctac gtaaaaaagg tgttgttggt
     1321 aaatttgtag agttctttgg tccaggtgta caacatttac cactagcaga ccgtgctaca
     1381 attgcaaaca tggcaccaga gtatggagca acttgcggat tcttcccggt tgatgatgaa
     1441 tctcttaaat atatgaagtt aactggtaga tcagacgaac atatcgcgct agtaaaagaa
     1501 tatttgaaac aaaaccatat gttctttgat gttgaaaaag aagatcctaa ttatacagat
     1561 gttatcgaat tggatttatc aacagttgaa gcatcgcttt caggaccaaa acgtcctcaa
     1621 gatttaattt tcttaagtga tatgaaatca tcatttgaaa attctgtaac agctccagca
     1681 ggcaaccaag gacacggttt agataaaagt gaatttgata agaaagctga aattaacttt
     1741 aaagatggat caaaagctac aatgaaaaca ggtgatattg caatagcagc aattacatca
     1801 tgtacaaata catctaaccc ttatgtaatg ttaggtgcag gtttagttgc taaaaaagca
     1861 gttgaaaaag gcttgaaagt tcctgaatac gttaaaactt ctctagcacc aggatcaaaa
     1921 gttgttaccg gatatttaag agatgctggc ttacaacctt atttagatga tttaggcttc
     1981 aacttggttg gttatggatg tacaacttgt atcggtaatt caggtccttt attaccagaa
     2041 attgaaaaag cgattgctga tgaggaccta ttagtgacat ctgtattatc tggtaaccgt
     2101 aactttgaag gtcgtatcca tcctcttgtt aaagccaatt acctagcttc accacagtta
     2161 gttgttgctt atgcattagc tggaacggtt gatattgatt tacagaatga acctattggt
     2221 aaaggtaatg acggtgaaga tgtatatttg aaagatattt ggccatcaat taaagaagtt
     2281 tcagataccg ttgatagtgt tgtaacacct gaattattta ttgaagaata taataacgta
     2341 tacaataaca acgaattatg gaatgagatt gatgtaactg atcaacctct atatgacttt
     2401 gatcctaatt caacatacat tcaaaatcca tcattcttcc aaggattatc taaagaaccg
     2461 ggtacgattg ttccattaaa tggtttacgt gttatgggta aattcggtga ttctgtgaca
     2521 actgaccaca tctctccagc aggtgcaatt ggtaaagata cgccagctgg taaatattta
     2581 caagatcatc aagtgcctat tcgtgaattt aattcatatg gttcaagacg tggtaatcac
     2641 gaagtaatgg ttcgaggtac gtttgctaat atacgtatta aaaaccaatt agcgccaggt
     2701 actgaaggtg gttttacaac ttattggcca acaaatgaag taatgcctat ctttgatgct
     2761 gcaatgaaat ataaagaaga tggtacaggt ttagttgtat tagctggtaa cgattatggt
     2821 atgggttcat ctcgtgactg ggcagcaaaa ggtacaaact tattaggtgt taaaacagtt
     2881 attgcacaaa gttatgaacg tatccatcgt tcaaatttag ttatgatggg tgtattacca
     2941 ttagagttta aaaaaggtga atcagctgat tctcttggtc tagatggtac agaagaaatt
     3001 tctgttaata ttgatgaaaa tgttcaacca catgactacg tcaaagttac tgctaagaag
     3061 caagatggtg atttggtaga atttgacgct atggttcgtt ttgactcact tgttgaaatg
     3121 gattactatc gtcacggtgg aattttacaa atggttttaa gaaataaatt agcgcaataa
     3181 aaaatagata tcacagtaaa attttaatcg gtatttgagg cactttgata gtgctcaaaa
     3241 atacttctat aaactttatg atataatagg tcggttcaaa acctttgtgt tcttggccgg
     3301 cctcttcttg tattttatgt tagaatgaga tataaagtta aactgaaaga aggcttaaaa
     3361 tgatatatag tattacagaa atagaagcgc gttatgctga aactgataag atgggtgtaa
     3421 tttatcacgg gaattatgca acttggtttg aagttgcgcg gttggattat atatcgaagt
     3481 taggttttag ttatgctgat atggaaaaac aaggaatcat ttcacctgtg actgacctca
     3541 atgtcaatta taaaaagtct attttttatc cagaaaaagt taaagttaaa acttgggttg
     3601 aaaaatattc gagattacgt tcagtgtata aatatgagat ttttaatgaa aaaggtgaac
     3661 ttgcaactac aggttccaca gaattgattt gcattaaaga agatactttt aagcctatac
     3721 ggttggatcg ttatttccca gattggcatg aagcttatag taaagtgcaa acgctcaata
     3781 atgaagggaa aacagtagag ataatggatg gtattgattc tttataagtt actgtaaatg
     3841 tttggagagg caatattaac aattaatcta taaaaaatag ccaatacgtt aacattaatg
     3901 taattagcgt gaagataagc taatatatag caaaaacgaa cttgttcata caacatcaga
     3961 tgtgctaaac caaagtacaa cttccgaggt tcaggacaca tcagaatgtg taaacaagtt
     4021 cgttttattt tagttatttt gtggaataag aaatttcatc ttcgtgatca actacattta
     4081 caataatgtg gtcatcttca aagtaccaca aatctttttc cgctacgaca acatttaaat
     4141 cgtcatattg ttgttcatag ccaatatcaa catcttcctt tggttcaact gtaaaagcag
     4201 gactaaatcc ttgcttgagt tggaattcgc caccatatct tacaaaaaac acgagcactt
     4261 tattattttc aggcaactca agttcatttt taaaccaagt tactgctgca tcagtaagtt
     4321 ctatttgcat aacaatgacc tcctatattt attacagaat gggagtgggc caacttgtat
     4381 tatttgtgga atttcttatc gaaattctct gtgttggagc ccaccccaac ctgctcatta
     4441 ttgtaagctg aatttccata agcttctatg ttgggacccc cgaccagaat tgaaaaaacc
     4501 tagttataag cgcatttcgt tcggttaact acagctaata taacattgtg gagcctatga
     4561 cattgattta tgtctcggta tcaaatgttc ttgtaaaaaa actaagtata gttgaatttt
     4621 aagttaaaaa aattgttttt gcaaaaataa aataatccat caactgcaaa aaaatatgca
     4681 atgatggact aatgtcaaaa aattgttaca tccattttat tttaggttct tcgcctctaa
     4741 aaatccttgc gatattagag cgatgtctaa ttatcaatat gattgaaact aagaaactaa
     4801 cgactaataa aatgtagtct tgaatgataa gcgagccaat cacacagcaa attgctgcaa
     4861 cgatacttgc taaagaaaca tatttaaaaa tcttcaatac aataaagaag ataattgcaa
     4921 gtattagtaa aagtatcgga ttgactccca agacgacacc tgcactagtt gcaaccgctt
     4981 tgccaccttg gaattttaaa taaacaggat aaacgtgtcc aagtatagcg aataagccaa
     5041 caattaaacc atttgtaaaa aaagtactaa tagggccatc tgcgtgaact ggtaaccata
     5101 aagggaagaa aacagttatg aaccctttga aaatatctag aaatgttacc aagaatcctg
     5161 caggacgacc taatactcta aagctattag tagcgccagt attaccacta ccaaattgtc
     5221 taatatcttt tttgaaaaat aattttccaa ttacgaatcc acttgggaaa gcgccgataa
     5281 gataacttag tagtaacatg acgattatca tcataaatat tacacatcct ttaatatctt
     5341 aggactattt tatcatattc gtttaaatta cggctaaaaa ttttaaaaac ggagattaat
     5401 atatggaatt aagctatgaa agttaattga tacttgcatt ttacgctgat ttatataaga
     5461 ataactattg tatagtttta aaaacgaacg tacgtttgca ggaggcgaaa tcattggcaa
     5521 tgaataaaca aaataattat tcagatgatt caatacaggt tttagagggg ttagaagcag
     5581 ttcgtaaaag acctggtatg tatattggat caactgataa acggggatta catcatctag
     5641 tatatgaaat tgtcgataac tccgtcgatg aagtattgaa tggttacggt aacgaaatag
     5701 atgtaacaat taataaagat ggtagtattt ctatagaaga taatggacgt ggtatgccaa
     5761 caggtataca taaatcaggt aaaccgacag tcgaagttat ctttactgtt ttacatgcag
     5821 gaggtaaatt tggacaaggc ggctataaaa cttcaggtgg tcttcacggt gttggtgctt
     5881 cagttgtaaa tgcattgagt gaatggcttg aagttgaaat ccatcgagat ggtaatatat
     5941 atcatcaaag ttttaaaaac ggtggttcgc catcttctgg tttagtgaaa aaaggtaaaa
     6001 ctaagaaaac aggtaccaaa gtaacattta aacctgatga cacaattttt aaagcatcta
     6061 catcatttaa ttttgatgtt ttaagcgaac gactacaaga gtctgcgttc ttattgaaaa
     6121 atttaaaaat aacgcttaat gatttacgca gtggtaaaga gcgtcaagag cattaccatt
     6181 atgaagaagg aatcaaagag tttgttagtt atgtcaatga aggaaaagaa gttttgcatg
     6241 acgtggctac attttcaggt gaagcaaatg gtatagaggt agacgtagct ttccaatata
     6301 atgatcaata ttcagaaagt attttaagtt ttgtaaataa tgtacgtact aaagatggtg
     6361 gtacacatga agttggtttt aaaacagcaa tgacacgtgt atttaatgat tatgcacgtc
     6421 gtattaatga acttaaaaca aaagataaaa acttagatgg taatgatatt cgtgaaggtt
     6481 taacagctgt tgtgtctgtt cgtattccag aagaattatt gcaatttgaa ggacaaacga
     6541 aatctaaatt gggtacttct gaagctagaa gtgctgttga ttcagttgtt gcagacaaat
     6601 tgccattcta tttagaagaa aaaggacaat tgtctaaatc acttgtgaaa aaagcgatta
     6661 aagcacaaca agcaagggaa gctgcacgta aagctcgtga agatgctcgt tcaggtaaga
     6721 aaaacaagcg taaagacact ttgctatctg gtaaattaac acctgcacaa agtaaaaaca
     6781 ctgaaaaaaa tgaattgtat ttagtcgaag gtgattctgc gggaggttca gcaaaacttg
     6841 gacgagaccg caaattccaa gcgatattac cattacgtgg taaggtaatt aatacagaga
     6901 aagcacgtct agaagatatt tttaaaaatg aagaaattaa tacaattatc cacacaatcg
     6961 gggcaggcgt tggtactgac tttaaaattg aagatagtaa ttataatcgt gtaattatta
     7021 tgactgatgc tgatactgat ggtgcgcata ttcaagtgct attgttaaca ttcttcttca
     7081 aatatatgaa accgcttgtt caagcaggtc gtgtatttat tgctttacct ccactttata
     7141 aattggaaaa aggtaaaggc aaaacaaagc gagttgaata cgcttggaca gacgaagagc
     7201 ttaataaatt gcaaaaagaa cttggtaaag gcttcacgtt acaacgttac aaaggtttgg
     7261 gtgaaatgaa ccctgaacaa ttatgggaaa cgacgatgaa cccagaaaca cgaactttaa
     7321 ttcgtgtaca agttgaagat gaagtgcgtt catctaaacg tgtaacaaca ttaatgggtg
     7381 acaaagtaca acctagacgt gaatggattg aaaagcatgt tgagtttggt atgcaagagg
     7441 accaaagtat tttagataat tctgaagtac aagtgcttga aaatgatcaa tttgatgagg
     7501 aggaaatcta gtgagtgaaa taattcaaga tttatcactt gaagatgttt taggtgatcg
     7561 ctttggaaga tatagtaaat atattattca agagcgtgca ttgccagatg ttcgtgatgg
     7621 tttaaaacca gtacaacgtc gtattttata tgcaatgtat tcaagtggta atacacacga
     7681 taaaaatttc cgtaaaagtg cgaaaacagt cggtgatgtt attggtcaat atcatccaca
     7741 tggagactcc tcagtgtacg aagcaatggt ccgtttaagt caagactgga agttacgaca
     7801 tgtcttaata gaaatgcatg gtaataatgg tagtatcgat aatgatccgc cagcggcaat
     7861 gcgttacact gaagctaagt taagcttact agctgaagag ttattacgtg atattaataa
     7921 agagacagtt tctttcattc caaactatga tgatacgaca ctcgaaccaa tggtattgcc
     7981 atcaagattt cctaacttac tagtgaatgg ttctacaggt atatctgcag gttacgcgac
     8041 agatatacca ccacataatt tagctgaagt gattcaagca acacttaaat atattgataa
     8101 tccggatatt acagtcaatc aattaatgaa atatattaaa ggtcctgatt ttccaactgg
     8161 tggtattatt caaggtattg atggtattaa aaaagcttat gaatcaggta aaggtagaat
     8221 tatagttcgc tctaaagttg aagaagaaac tttacgcaat ggacgtaaac agttaattat
     8281 tactgaaatt ccatatgaag tgaacaaaag tagcttagta aaacgtatcg atgaattacg
     8341 tgctgacaaa aaagtcgatg gtatcgttga agtacgtgat gaaactgata gaactggttt
     8401 acgaatagca attgaattga aaaaagatgt gaacagtgaa tcaatcaaaa attatcttta
     8461 taaaaactct gatttacaga tttcatataa tttcaacatg gtcgctatta gtgatggtcg
     8521 tccaaaattg atgggtattc gtcaaattat agatagttat ttgaatcacc aaattgaggt
     8581 tgttgcaaat agaacgaagt ttgaattaga taatgcagaa aaacgtatgc atatcgttga
     8641 aggtttgatt aaagcgttgt caattttaga taaagtaatc gaattgattc gtagctctaa
     8701 aaacaagcgt gacgctaaag aaaaccttat cgaagtattc gagttcacag aagaacaggc
     8761 tgaagcaatt gtaatgttac agttatatcg tttaacaaat actgacatag ttgcgcttga
     8821 aggtgaacat aaagaacttg aagcattaat caaacaatta cgtcatattc ttgataacca
     8881 tgatgcatta ttgaatgtca taaaagaaga attgaatgaa attaaaaaga aattcaaatc
     8941 tgaacgactg tctttaattg aagcagaaat tgaagaaatt aaaattgaca aagaagttat
     9001 ggtgcctagt gaagaagtta ttttaagtat gacacgtcat ggatatatta aacgtacttc
     9061 tattcgtagc tttaatgcta gcggtgttga agatattggt ttaaaagatg gtgacagttt
     9121 acttaaacat caagaagtaa atacgcaaga taccgtacta gtatttacaa ataaaggtcg
     9181 ttatctattt ataccggttc ataaattagc agatattcgt tggaaagaat tgggacaaca
     9241 tgtatcacaa atagttccta tcgaagaaga tgaagtggtt attaatgtct ttaatgaaaa
     9301 ggactttaat acagatgcat tttatgtttt tgcgactcaa aatggcatga ttaagaaaag
     9361 tacagtgcct ctatttaaaa caacgcgttt taataaacct ttaattgcta ctaaagttaa
     9421 agaaaatgat gatttgatta gtgttatgcg ctttgaaaaa gatcaattaa ttaccgtcat
     9481 tactaataaa ggtatgtcat taacgtataa tacaagtgaa ctatcagata ccggattaag
     9541 ggcagctggt gttaaatcaa taaatcttaa agctgaagat ttcgttgtta tgacagaagg
     9601 tgtttctgaa aatgatacta tattgatggc cacacaacgc ggctcgttaa aacgtattag
     9661 ttttaaaatc ttacaagttg ctaaaagagc acaacgtgga ataactttat taaaagaatt
     9721 aaagaaaaat ccacatcgta ttgtagctgc acatgtagtg acaggtgaac atagtcaata
     9781 tacattatat tcaaaatcaa atgaagaaca tggtttaatt aatgatattc ataaatctga
     9841 acaatataca aatggctcat tcattgtaga tacagatgat tttggtgaag taatagacat
     9901 gtatattagc taaaaactat atgcaatcac gaaattaaat gataaaatac agtaatgtta
     9961 aattttgact aaattcaagg gatttatatt aaatgctgac caagtactta tcgttaaatt
    10021 agcgatatac tttgaccaaa ctaatataaa tctcttgtat tgtataagaa atgatgatgt
    10081 atctctatat ttactgaagg ttcaattcag tctaatttta tagaaggtac atgattcatg
    10141 tagaggatgc gaggggttta ttttgaaaga tttcgatagt ttaatacctg gatggtttaa
    10201 agaatttgtc catgttggta ccgatttaat atggtctcaa tatttaattg gtctattatt
    10261 gacagctgga ttcttcttta cgattagttc taaattcgtt caattacgaa tgttacctga
    10321 aatgtttaga gctttagtag aacgtccaga aactttagaa gatggtaaga agggtatttc
    10381 gccattccaa gcatttgcga ttagtgctgg ttcgagagtt ggtactggta atattgctgg
    10441 tgttgcgact gcgattgttt taggcggtcc aggtgcagta ttttggatgt gggttattgc
    10501 atttataggt gcagcgagtg catttataga agcgactttg gctcaggttt ataaagtaca
    10561 tgataaagat ggtggattcc gtggtggtcc agcttactat attactaaag gtttaaatca
    10621 aaaatggcta ggtatcgtat ttgcgatttt aattacaatt acatttgcat ttgtatttaa
    10681 cacagtgcaa tctaatacaa ttgcggagtc gttaaatacg caatataata ttagtccagt
    10741 aatcacaggt attattttag caatcgtaac agctattatt atatttggtg gtgtacgtag
    10801 tattgctacg ttatcttcgt taattgtacc gattatggct atcatttaca ttggtatggt
    10861 tttagtaata ttgctattta atttagatca aattgttcct atgataggta cgattattaa
    10921 aagtgcattt ggtattgaac aagtaactgg tggcgctgta ggtgctgcgg ttcttcaagg
    10981 tatcaaacgt ggtttattct ctaacgaagc tggtatgggt tctgcgccga atgcagcggc
    11041 aactgctgcc gtaccacacc ctgttaagca aggtttaatc caatcattag gtgtgttctt
    11101 tgatacaatg ttggtttgta cagcaactgc aatcatgatt ttactatatt caggattgaa
    11161 atttggtgat aacgcacctc aaggtgttgc agttactcaa tcagcactta atgagcattt
    11221 aggttctgct ggaggtattt tcttaacaat agcagttaca ctgtttgcat tttcatctgt
    11281 tgtaggtaat tactattacg gtcaatctaa tattgaattt ttatcaacaa accgtgtaat
    11341 attatttatc tttaggtgtc ttgttgtagt acttgtcttt gtcggtgcag ttgtaaaaac
    11401 agaaacagta tggaatacgg cagacttatt tatgggctta atggcaattg taaacattat
    11461 ttccattata ggactgtcca atgtagcttt tgcattgatg aaagattatc aaaagcagaa
    11521 aaaagaaggc aagaaccctg tctttaaacc tgaaaactta gaaattaact tatttggaat
    11581 tagtgcttgg ggcgctaaca aatataagaa ctctgataaa taaatatttt taatgtaacg
    11641 attcattata taaaaatcaa ataaaccctg aggttttcaa attaaagcga aaatctcagg
    11701 gttttaaatt tgtactcaat aatcatctgt atcattacac tagttaatct aagatttgat
    11761 tatatattaa aatagtaagg ttgagattta tttcctccat tggtgtgcaa agattatacc
    11821 attgataaga catattgttg gatagatgaa tataaattta ttttagcttt gcacaattta
    11881 atagtacgtt ttagtttgta ttgagtatta attcagataa tatataaata aatagaatta
    11941 tttgtaaata tgggagtcag atttttttat tatagttatt ccactttgag aggcatcgaa
    12001 attaaattaa gatatatcat atagggtagg cgtgtaatag aacacaagta tttaaattat
    12061 tatactttca agttaga
//
LOCUS       DAHHYJ010000082        11979 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000082 DAHHYJ010000000
VERSION     DAHHYJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11979
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.082"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            410..1810
                     /locus_tag="NBK22_001691"
     CDS             410..1810
                     /locus_tag="NBK22_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2414876.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKRRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            2354..3730
                     /locus_tag="NBK22_001692"
     CDS             2354..3730
                     /locus_tag="NBK22_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCD2414877.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQNIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCHKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTNHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSGDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMM
                     SMYLRGEL"
     gene            3732..4871
                     /locus_tag="NBK22_001693"
     CDS             3732..4871
                     /locus_tag="NBK22_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCD2414878.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4846..5211
                     /locus_tag="NBK22_001694"
     CDS             4846..5211
                     /locus_tag="NBK22_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCD2414879.1"
                     /translation="MQHYLLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WPKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGHLEDIECRQTRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            5214..5483
                     /locus_tag="NBK22_001695"
     CDS             5214..5483
                     /locus_tag="NBK22_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414880.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEHEIIAINDDGEIKIKISLSTKK"
     gene            complement(5684..5851)
                     /locus_tag="NBK22_001696"
     CDS             complement(5684..5851)
                     /locus_tag="NBK22_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCD2414881.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
     gene            complement(6358..7188)
                     /gene="thiD"
                     /locus_tag="NBK22_001697"
     CDS             complement(6358..7188)
                     /gene="thiD"
                     /locus_tag="NBK22_001697"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD2414882.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
     gene            7372..8028
                     /locus_tag="NBK22_001698"
     CDS             7372..8028
                     /locus_tag="NBK22_001698"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD2414883.1"
                     /translation="MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            8029..8409
                     /locus_tag="NBK22_001699"
     CDS             8029..8409
                     /locus_tag="NBK22_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCD2414884.1"
                     /translation="MLNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            8542..8910
                     /locus_tag="NBK22_001700"
     CDS             8542..8910
                     /locus_tag="NBK22_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCD2414885.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            9031..10515
                     /locus_tag="NBK22_001701"
     CDS             9031..10515
                     /locus_tag="NBK22_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD2414886.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(10656..11108)
                     /locus_tag="NBK22_001702"
     CDS             complement(10656..11108)
                     /locus_tag="NBK22_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD2414887.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALSLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(11121..11885)
                     /locus_tag="NBK22_001703"
     CDS             complement(11121..11885)
                     /locus_tag="NBK22_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCD2414888.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
BASE COUNT         4051 a         1832 c         2174 g         3922 t
ORIGIN      
        1 aaataaatat ttattaaaca ttatgaattt ttaaagagta atgtctgact cgttgatcat
       61 ttatttttgt aaaaataaat taaagtaatg acaaagttat tgaagtaaat tgagtataaa
      121 catttaaata cgatgtcgaa aatggcgata gcatatgact tacatgaagt tgtgtgctat
      181 cgctattttt agttataatt ccaaaaagtt aatcgttcga tgatttaaga attattattg
      241 tttaattcaa atgtatgagg gtataaaatc attgaattta attcgataaa gcgaaatttt
      301 tgaacaaaca tacttttgta tttatataaa agttttaatt cttataaatt tgacaaaact
      361 aattaactcc gtataattat gaaacataca agagggagtg tatgaattca tggattttaa
      421 taaagagaat attaacatgg tggatgcaaa gaaagctaaa aaaaccgttg ttgcaaccgg
      481 tatcggtaat gcaatggaat ggttcgattt tggtgtctat gcatatacaa ctgcgtacat
      541 tggagcgaac ttcttctctc cagtagagaa tgcagacatt cgacaaatgt tgactttcgc
      601 agcattagcc attgcgtttt tattaagacc aattggtggt gtcgtatttg gtattattgg
      661 tgacaaatat ggacgtaaag ttgtattaac atctacaatt attttaatgg cattttcaac
      721 attaaccatt ggattattgc caagctatga tcaaattgga ctttgggcac caatactatt
      781 attgcttgca agagtactac aagggttttc aacaggtgga gagtatgcgg gggcaatgac
      841 atatgttgcc gaatcatctc cagataagcg tcgtaactca ttaggtagtg gactagaaat
      901 tgggacatta tcaggttaca tagctgcttc aattatgatt gctgtattaa cattcttttt
      961 aacagatgaa caaatggcat catttggttg gagaatccca ttcttactcg gtttattcct
     1021 aggattattc ggcttatatt tacgtcgtaa gctggaagaa tcaccagttt tcgaaaatga
     1081 tgttgcaaca caaccagaaa gagataacat taacttttta caaatcatca gattttatta
     1141 caaagatata tttgtatgtt ttgtagctgt tgtattcttc aatgttacaa actatatggt
     1201 aactgcatat ttaccaacct atttagaaca agttattaaa ttagatgcaa cgacaacaag
     1261 tgtattaatt acttgtgtca tggcaataat gattccatta gcattaatgt ttggtaagtt
     1321 agcggataaa ataggtgaaa agaaagtatt tctaattggt actggtgggc taacattatt
     1381 cagtatcatc gcatttatgt tattacattc acaatcattt gttgtaatag taatcggtat
     1441 atttatatta ggatttttct tatcaactta cgaagcgaca atgccagggt cgttaccaac
     1501 gatgttttac agtcatataa gatatcgaac tttatcagta acatttaata tctctgtttc
     1561 gatatttggt ggtacgacgc cattagttgc aacatggtta gttacgaaaa ctggagatcc
     1621 attagcacct gcgtattatt taacagcaat cagtgttatt ggctttttag ttattacatt
     1681 cttacattta agtacagcag gaaaatctct aaaaggttcg tatccaaatg tagataacga
     1741 gcaagataga gcttattatg cagaacatcc aaaagaagca ttatggtggg ttaaagaacg
     1801 taagaattag agattttaat aaaaagtata aatcaatcgt atataagcac tttaaagcta
     1861 gtaggttctg ctaactttaa agtgcttttt aaattaagaa ctgtaattag ccgtaataaa
     1921 gtttttgtat atacataaac ccccactgca atgattatcg caatggggga aagaggggac
     1981 ttaaagcata tgtttagctt tgaatactta aaattctctt gctattgaaa tgttaggatg
     2041 taaatatgtc ttagagtatt ttgtccaacg caattaatat tgagactcta accttcaata
     2101 ttattataga gaacacaaac ttaaatagat tgggtgactt atttgtgtca gttattgcga
     2161 ttgcgataac ttcttttctc tatatacata tagtaacgtc ttatctaata aaaaacatgg
     2221 tactacagta tcaaatttat ctagggctta agtttgattt ttataatagg caggtttacc
     2281 tgataaaaat acttattcat tatataatgt taacaatatg tattttaaag tttacattga
     2341 gtgagggata ttgatgaacg taattttaga acagttgaaa acacatactc aaaataaacc
     2401 taatgacata gcattacata tcgatgatga aacaattaca tatagtcaac taaatgcccg
     2461 catcactagc gcagttgaat ctttgcagaa atattcactt aaccctgtcg ttgctattaa
     2521 tatgaaatca ccggtgcaaa gtattatttg ttatttagct ttgcatcgtt tacataaagt
     2581 gcctatgatg atggaaggta aatggcaaag tactatacat cgtcaattga ttgaaaaata
     2641 tggtattaaa gatgtaattg gagatacagg tctcatgcag aatatagact caccgatgtt
     2701 tattgattca acgcaattac agcactaccc caatttatta catattggtt ttacttcagg
     2761 gacaactgga ctgccaaaag catattatcg tgatgaagat tcatggttgg cttcttttga
     2821 agttaatgaa atgttgatgt taaaaaatga aaatgcaata gcagcccctg gaccactatc
     2881 gcactcgtta acattatatg cgttattgtt tgctttaagt tccggtcgta cttttatagg
     2941 acagaccact tttcatcctg aaaagttact taatcaatgt cataaaatat catcatacaa
     3001 agttgctatg tttcttgttc caacgatgat taaatcatta ttgttagttt acaacaatga
     3061 acatacaatc caatcatttt ttagcagtgg agataagctg cattcttcta tttttaaaaa
     3121 gataaaaaat caagcaaatg acataaattt gattgaattt tttggtacat cggaaaccag
     3181 ttttatcagc tataacttga atcagcaagc accagttgaa tcagtaggtg tgctatttcc
     3241 aaatgtggaa ttgaaaacaa cgaatcacga tcacaatggt ataggaacta tttgtataaa
     3301 aagtaatatg atgtttagtg gctatgtaag tgaacaatgt ataaataatg atgaatggtt
     3361 tgttactaat gataatggct atgtaaaaga gcagtattta tatttaacgg gacgtcaaca
     3421 ggatatgtta attattggtg gtcaaaatat atatccagca catgttgaac gccttttaac
     3481 gcaatcttcg agcattgatg aagcaattat catcggtatt ccaaatgagc gttttggtca
     3541 aataggcgta ttgctttatt ctggtgatgt gacacttaca cataaaaatg taaaacaatt
     3601 tttaaaaaag aaagtgaaac gctatgaaat tccatcgatg attcatcatg tagaaaagat
     3661 gtattacact gcaagtggta aaattgctag agaaaaaatg atgtcgatgt atttgagagg
     3721 tgaattataa tatgaatcaa gcagtcatag ttgcagctaa acgaactgca tttgggaaat
     3781 atggtggcac tttaaaacat ttagagccag aacaattgct taaaccttta ttccaacatt
     3841 ttaaagagaa gtatccagag gtaatatcta aaatagatga tgtagtttta ggtaatgttg
     3901 ttgggaatgg tggcaatatt gcaagaaaag cattgcttga agcggggctt aaagattcaa
     3961 tacctggcgt cacaatcgat cggcaatgtg ggtctggact tgaaagtgtt caatatgcat
     4021 gtcgcatgat ccaagccgga gctggcaagg tatatattgc aggtggtgtt gaaagtacaa
     4081 gtcgagcacc ttggaaaatc aaacgaccgc attctgtgta cgaaacagca ttacctgagt
     4141 tttatgagcg tgcatcattt gcacctgaaa tgagcgaccc atcaatgatt caaggtgctg
     4201 aaaatgtggc caagatgtat gatgtttcaa gagaattaca agatgaattt gcttatcgaa
     4261 gtcatcaatt gacagcggaa aatgtaaaga atggaaatat ttctcaggaa atattaccta
     4321 taaccgttaa aggagaaata ttcaacactg atgaaagtct aaaatcacat attccgaaag
     4381 ataactttgg ccgatttaag cccgtgatca aaggtgggac cgttaccgct gcgaatagtt
     4441 gtatgaaaaa tgatggtgca gttttattgc ttattatgga aaaagatatg gcatacgaat
     4501 taggtttcga gcatggttta ttatttaaag atggtgttac ggtaggtgtt gattctaatt
     4561 ttcctggcat tggtccagta ccagccattt ccaacttact aaaaagaaat caattaacga
     4621 tagaaaatat tgaagtcatt gaaattaacg aagcgttcag tgcacaggta gttgcctgcc
     4681 aacaagcttt aaatatttca aatacgcaat taaatatatg gggtggtgca ttagcatcag
     4741 gtcatccata cggtgcaagc ggtgcccaat tagtgactcg attattttat atgtttgaca
     4801 aagagactat gattgcatct atggggatag ggggaggtct aggaaatgca gcattattta
     4861 ctcgattcta accagcgatt aaatgtgtca ttttctaagg atagtgtggc tgcatattat
     4921 cagtgtttta accaacctta tagaaaagaa gtaccaccat taatgtgtgc gtcattatgg
     4981 ccaaaatttg atttatttaa aaaatatgca aatagcgaac tgattttaac aaaatcagca
     5041 attaatcaaa ctcaaaagat agaagtagac acaatatatg tagggcattt agaagatatt
     5101 gaatgccgac agactcgcaa tatcacacgt tatacaatgg ctttaacatt aactaaaaat
     5161 gatcaacatg tcataacggt tacacaaact tttattaagg cgatgaagta gagatgaagt
     5221 ttaatgagat atggataaat gaatatttgg cgctcgtaaa tgatgataat ccaatacata
     5281 atgagattgt gccaggacaa ttagtgagtc aaatgatgct gatggctatg tcattagaga
     5341 caaaccagtg tcaaattaac tacgttaaac ctattttaat aaatgaaaat atcgaattca
     5401 ttgaacaaca cgaacacgaa attatagcaa ttaatgacga tggagagatt aaaataaaaa
     5461 tttctttgag cacaaaaaaa taaccgatat tagctgcatg aacgcatatt aattaggaga
     5521 tgaaaggaca gctaatatca gttatgtatt gttattatta ttgggaacag agatgaatat
     5581 aggttacgtt tctttctttg cacggggatg cattaatcta aaataataat aacaactata
     5641 tcaatgttta ataaattctg gattattgga acgattagtc aatttaacta actttcatat
     5701 gatctatatc gtcttgtaat aaagagagca atttgaatat ttcagtatca ctaaatgaat
     5761 cgtcacattt aattgaaaca tgctgaaacg ttttggttat aatttcataa actggtgcgc
     5821 cttcatggtg atactgtcga taaataatca taacctatat tacctccttt gctactctat
     5881 ggttatatta taaataacat ttttatgtgt gacatcaacc ttaagtatca actttttatc
     5941 agacatagaa cgtatgattt actaagacta tttatgtata aaagttctaa ataaatatat
     6001 atttatagag tcgcctggca gtcatttggg aaatataaca tatatgatta gagaggcatc
     6061 tatcgcaaaa gaatgataat gatagaggta ttgagcatat agatgagttt aagttcatct
     6121 tgaaaataaa gggttattta gtcatagatg tagatgtata ggaaatattt gtatgtattg
     6181 ttcgatatgt atgaaatttt caataaaagc taataacgct tatatgtaac tttcaaattt
     6241 aaattatata cagagcatga tgattataaa aaaataacca catcacataa attgagttca
     6301 tacccaattt aagtggtgtg gctaataatg ttgatttata gatgaaccgc ctaatcgtta
     6361 aacctctgtt acttcaacat cgatatgttc aatacggttg tatgcaccgt gatccacagg
     6421 accaacaaaa tcattcattt tccaaccgtt tttaatagca gaagcgacga aagctttcgc
     6481 gctaatcaca gcttctttcg gtgacttacc gttagctaaa tatgcagttg ttgccgcagc
     6541 aaatgtacaa ccagcaccat ggttataact ttgttggaac atgtctgttg ttagttgata
     6601 aaatgtttga ccatcatagt ataagtcata cgatttatct tgatctaaag ctttgccacc
     6661 tttaatgatg acatgctgtg cgcctttatc aaagataatt gttgcagcct ttttcatatc
     6721 ttcaattgaa tttaatttac ctaatcctga taattgaccc gcttcaaata agtttggtgt
     6781 cactaccgtt gctttaggta gtaaatattt aatcatcgcc tcagtatttc caggattaag
     6841 cacttcatct tcgcctttac aaaccatgac aggatctact acaaaatatt gtgcattaga
     6901 tgcctcatat acttctccag cacgtttgat tatctcctca gtacctaaca tacctgtttt
     6961 aatagcatca ggtccgattg ataaagccgt ttcaagttgc ttttcaaata cgtccatagg
     7021 taatggtgtg acatcgtgtg accatgtatc tttatccata gtaacgatgg cagttaaagc
     7081 gaccatgcca tacgtatcta attcttggaa cgttttcaaa tctgcttgca tacctgcgcc
     7141 agcacttgtg tcagaaccgg caattgttaa aactttcttt aaagccattg agcttcactc
     7201 ctacataata atattgtatt catcatatca tttttaacct aattgaaaaa tattaagcat
     7261 tcaatatttg atgattgttg aaatgaatca ttcatactat tgtaactttt gaaaatgtca
     7321 ttcactttag ataagtgtga tatgttaaaa tatgtcctga ggtgagattg aatggaatgg
     7381 tcgcaaattt ttcatgacat aacaacgaaa catgacttta aagctatgca tgatttttta
     7441 gaaaaagaat attcgactgc aatcgtatac cctgataggg aaaatatata tcaagcgttt
     7501 gatttaacac cgtttgaaaa tatcaaagtt gttatattag gacaagaccc gtatcatggt
     7561 ccaaaccaag cacatggatt agcattttca gtgcaaccta acgcaaaatt ccctccatct
     7621 ttacgtaata tgtataaaga attagcagat gatattggat gcgttagaca aacaccgcat
     7681 ttacaagatt gggcaagaga aggcgtcttg ttattgaata cagttttaac cgtaagacag
     7741 ggtgaagcaa attctcatcg tgatattggt tgggaaacat ttactgatga aattattaaa
     7801 gcagtgtctg attataaaga acatgttgtc tttattttgt gggggaaacc tgcacagcaa
     7861 aaaataaagc ttatcgatac atctaaacat tgtattataa aatcagtgca tcctagtcca
     7921 ctgtctgcat atagaggatt ctttggatca aaaccgtatt ccaaagcgaa tgcctattta
     7981 gagtcagtag gaaaatcacc aattaattgg tgtgaaagtg aggcgtagat gttgaataga
     8041 gaaactttaa tagcacgaat tgagcaagaa ttagtacaag cagagcaggc acagcatgac
     8101 catgactttg aaaaacatat gtatgccata catatattaa catctttata tgcttcaaca
     8161 tcaaatacac cacatattgg tgaacaacaa atgaatcgtc gtattgctaa ccataatcaa
     8221 atgccacaat cacaaataac gcagccaact catcaagtga cagctgctga aattgaagcg
     8281 atgggtggta aagtaaatac gcattcagca catcatcata ataagtcata ttcacaacct
     8341 tcaaaccaac aacaaagatt agcgacagat gatgacattg gcaatggtga atccatattt
     8401 gatttttaaa aagcaacaat gaaacataat tacttaatag cttgttaagt atgtaggtta
     8461 ataatcaaga cgcatatact tttattcgag tgttcggatt taaacattta ttaatactga
     8521 attatataag gagaggtagc aatgaaatta tttattattt taggtgcatt aaacgcgatg
     8581 atggctgtcg gaacaggtgc atttggtgcg catggtttac aaggaaaaat aagtgatcac
     8641 tatttatcag tatgggaaaa agcaacgacg tatcaaatgt accatggctt agcattatta
     8701 attataggtg taattagtgg tacaacttca atcaatgtta actgggctgg ctggttaata
     8761 tttgctggta ttattttctt tagtggatca ttatatattt tagtattaac tcaaattaaa
     8821 gttttaggtg cgattacgcc aattggtggc gtattgttca tcattggatg gataatgtta
     8881 atcattgcga cattcaaatt tgctggttaa attttaaaac tttagattac ctatgtaact
     8941 aaacattaaa tttttaataa aaataatcaa gaaaaagagt tacaaactca tcttttgggt
     9001 atagaatacc ttcgaggtga gtttttattt atggaaaaaa agaataagca aatagataga
     9061 ggcgatttaa aacaaaacct atctgaaaag tttgtatggg cgattgcata tggttcatgt
     9121 atcggatggg gcgcattcat cttaccagga gactggatta agcagtcagg tccgattgca
     9181 gcatcaattg gtatagttat tggtgcatta ttaatgatat taattgcggt tagttatggc
     9241 gcattagtag agagatttcc agtatcaggg ggcgcgtttg cctttagttt cttaagtttc
     9301 ggcagatatg tgagtttctt ctcatcatgg tttttaactt ttggttatgt ctgtgtcgtt
     9361 gctttaaatg cgaccgcatt cagtttacta gttaaattct tattgccaga tgtcttaaat
     9421 aatgggaaac tatacaccat tgcgggctgg gacgtttata ttacggaaat cattattgcg
     9481 accgtattac tacttgtatt catgctagta acgattcgtg gcgcaagtgt atctggatca
     9541 ttacaatatt atttctgtgt ggcgatggta atcgtcgtat tattgatgtt ctttggttca
     9601 ttctttggta ataattttgc acttgaaaat ttacaaccgt tagctgaacc tagcaaagga
     9661 tggttagtgt ctattgtggt tattgtatcc gtggcaccat gggcatatgt tggatttgat
     9721 aatattccac aaacagcaga agagtttaac tttgcaccaa acaagacatt taagcttatc
     9781 gtgtacagtt tattagcagc atcattaact tatgttgtca tgattttata cactggttgg
     9841 ttatcaacaa gtcatcaaag tttaaatggg cagttgtggt taacaggtgc tgttacacaa
     9901 acagcatttg gttatattgg attaggtgta ttagcaattg caattatgat gggtatattt
     9961 actggtttaa atggattctt gatgagttca agtcgcttgt tattttctat gggacgttca
    10021 ggtattatgc caacaatgtt tagtaaatta catagtaaat acaaaacacc atatgtcgca
    10081 atcatattcc tagtaggtgt gtcgttaatt gcaccttggc taggaagaac tgcattgact
    10141 tggattgtag atatgtcatc tacaggtgta tccattgcct actttattac atgtttgtct
    10201 gcagcgaaat tattcagtta taacaaacaa agtaatacgt atgcaccggt ttacaaaacg
    10261 tttgctatta tcggctcatt tgtatcattc attttcttag cgttgttatt agtgccaggt
    10321 tctcctgcag cactgactgc accgtcttat attgcattac ttggatggtt aatcatcggt
    10381 ttaatattct ttgtgattcg atatcctaaa ttgaaaaata tggataatga tgaattaagt
    10441 cgcttgattt taaatagaag tgaaaatgaa gttgatgata tgattgaaga acctgaaaaa
    10501 gaaaaaacta aataataaaa gaatcgcaca atataccttc ttcattcgga ggcgtatcgt
    10561 gcgatttttt gtattataaa ttgacattta agacgaggca gctgaacctt atatataatt
    10621 gctaatagtt tgggctgagc caattctaac aatatttata atcgtttaaa agatttcacg
    10681 aacccagaaa caattaattt agaaatttgg tcggcgaata ataaacctaa tgcgatggct
    10741 cctgcaataa gtgttacttc taacattgta tttattgctg tgctgaaatt taataagact
    10801 aaattttttg tagcatcgta tgctaagcca ccaggtacta atggaatgat acccgttacc
    10861 ataaaaatga tggcaggttc tttttgttta cgagccatat aatgacttaa caagcttaat
    10921 gctaaactac caaagaaact agagtatata gtgtgcacat taaagccgtt gaagaataag
    10981 gtgtaaacca tccatccaca tgtaccaacg aaaccacatg atagatataa ttttctaggt
    11041 gcatcaaaaa tgacgcagaa gaacattgaa gctaaaaagc taaagataaa gtttaagatc
    11101 caaaacatag tctgatactc ctatactaaa attaatacgc taccaacgcc agcaccgatg
    11161 ccaaacgcag taaccaatgc ttctaatgat ttcgttgtga acatcaacat gtgtccacca
    11221 aataaatctt gtattgcgtt tgttattaat acaccaggaa caataggcat gactgctgca
    11281 atgataatag ttgccaagtc acctgttggg ataagtgtat gtccaataac ggcgataatc
    11341 ccaataacta atgaaccaat gaattctggg ataaactgtg catgtaactt gcgatctaaa
    11401 atctcagtga ctaggtatcc tagactacct gctaatatcg cagttaaaac atcaatcaat
    11461 ctaccacctt gtaaatataa gaaactcatt gcaatcattg ctgcagcaaa acctttaaag
    11521 ggaagactgc tgtcacgctt agcaacatat attttttcaa gttgcgtttt tgcttcggct
    11581 aaagaaattt cattgtttgt aatttgacgc gaaattttat tagcttgcga aatttttatt
    11641 aagtttgtat ctcgagaggt aattctaaat attctaggaa acgattccga atgtaacgta
    11701 aactggatga cagtgtttgt aacaaagctg ttactttcac tgtaaccaag tttttttgca
    11761 atacgtgtca tggtatcttc tacacgcgta ccttctgcac cagattctaa tagtatgcga
    11821 gcagcaagca tgacaacgtc tttgataagt acctcttgtt tgtattcttc tgaatttatg
    11881 tccatttcat caccattgtt tataagaatt taatactcat tatagtttat acactataaa
    11941 ataaccacat gagctttttg ataagttgtt attgatttt
//
LOCUS       DAHHYJ010000083        11488 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000083 DAHHYJ010000000
VERSION     DAHHYJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.083"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..708
                     /locus_tag="NBK22_001704"
     CDS             <1..708
                     /locus_tag="NBK22_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2414889.1"
                     /translation="YGGMILLPIYLQNLRGFSALDSGLLLLPGSLIMGLLGPFAGKLL
                     DTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMVTAAI
                     NALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNPVVQD
                     HMRELASQYGGQEGAMKVLLQFVNKLATVEGINDAFIVATIFSIIALILCLFLQSNKK
                     AKATAQKLEASSNNNHE"
     gene            complement(954..1592)
                     /locus_tag="NBK22_001705"
     CDS             complement(954..1592)
                     /locus_tag="NBK22_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414890.1"
                     /translation="MTNPNQRLEPFDETFQQSNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADALKKYYLNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            1800..2720
                     /locus_tag="NBK22_001706"
     CDS             1800..2720
                     /locus_tag="NBK22_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2414891.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKREIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGPQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            complement(2828..3247)
                     /locus_tag="NBK22_001707"
     CDS             complement(2828..3247)
                     /locus_tag="NBK22_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414892.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(3244..3735)
                     /locus_tag="NBK22_001708"
     CDS             complement(3244..3735)
                     /locus_tag="NBK22_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCD2414893.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDIFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            3832..4779
                     /gene="corA"
                     /locus_tag="NBK22_001709"
     CDS             3832..4779
                     /gene="corA"
                     /locus_tag="NBK22_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCD2414894.1"
                     /translation="MALKIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSPIALNIFIQDNVLV
                     TYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYFNFVYALEDSVYHFED
                     RHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNGDLIIDNKHSLYIQHI
                     DDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLVSVIFSPLTFIAGIYG
                     MNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            4810..5859
                     /locus_tag="NBK22_001710"
     CDS             4810..5859
                     /locus_tag="NBK22_001710"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCD2414895.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(5907..7115)
                     /gene="gltS"
                     /locus_tag="NBK22_001711"
     CDS             complement(5907..7115)
                     /gene="gltS"
                     /locus_tag="NBK22_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCD2414896.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVATLDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTAQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(7452..8060)
                     /locus_tag="NBK22_001712"
     CDS             complement(7452..8060)
                     /locus_tag="NBK22_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCD2414897.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            8459..9106
                     /locus_tag="NBK22_001713"
     CDS             8459..9106
                     /locus_tag="NBK22_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCD2414898.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMALWHLILMV
                     PAVCVLIINLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTVFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVLLYFVYLGLTI
                     VMIVFLCFDSKPANKYGESPKYPSTIKNQPVTSETPEKACTSETPKTPEETDNQL"
     gene            9337..10236
                     /locus_tag="NBK22_001714"
     CDS             9337..10236
                     /locus_tag="NBK22_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414899.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            10229..11461
                     /locus_tag="NBK22_001715"
     CDS             10229..11461
                     /locus_tag="NBK22_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414900.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGRLALITSYIPFLAPFQLVVRAQTSGLQIHEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
BASE COUNT         4046 a         1690 c         2011 g         3741 t
ORIGIN      
        1 tatggtggta tgattttatt accgatttat ttacaaaatt tacgcggatt ctcagcatta
       61 gattccggat tgttattatt acctggttct ctaattatgg gtctacttgg accatttgca
      121 ggtaaattgt tagatactat tggtttaaaa ccattagcta tctttggtat tgctgtcatg
      181 acttatgcaa catgggaatt aactaaatta aatatggata caccatatat gacaatcatg
      241 ggtatctatg tacttcgttc atttggtatg gcatttataa tgatgccaat ggtaactgca
      301 gctattaatg cgttaccggg acgacttgcc tctcatggta atgctttctt aaatacgatg
      361 cgtcaattag caggctctat aggtacagca atcttggtta ctgtaatgac aacacaaact
      421 acacaacact tatcagcttt tggggaagag ttagataaaa cgaatcctgt tgtacaagat
      481 catatgcgtg aattagcatc acaatatggc ggacaagaag gcgcaatgaa agtgttacta
      541 caatttgtaa ataaactagc aacggttgaa ggtattaatg atgcatttat agttgcaacg
      601 atatttagca tcatcgcctt aattttatgt ttatttttac aaagtaataa aaaagcaaaa
      661 gctacagctc aaaagttaga agcaagctca aataacaatc atgaataaat aaaataaatt
      721 aattgaagtg tgactaatca aaaattatgt tgtggggaca tgatttttaa agtatcggtg
      781 ccaaatatgg ttatcgatac tttttttatt tgttgattta taaaataaaa gaggaattat
      841 attaaaattt ggcattgacg tagtaggtca ttaataaaga aaaagcagga agtgggtcaa
      901 cgaaatgaat tttgtgaaaa taacatttct gtcccaatcc ctactatata acattatttt
      961 aaacgaggca tgcgattacg gaagaataag cttataacaa gtaaaccgat gctacagccc
     1021 agtaaaatga tgccgttatg aatagcgtca gcttctgtaa tcacttgatc tggtggtaca
     1081 tttaaataat atttttttaa agcatctgca atttcttgtg cgagtgcttt aataatataa
     1141 ctgaaataca atgtaatgac taacataatt gttaacacta aaatatttac tacttttaga
     1201 actgtgcgat taaatacaat tgtcagtatt gttatgacta ggctaacgat acataaaata
     1261 aagaaaccgt taaagactaa aattaagcga tcgattcctt gttgtaaatc attgatatta
     1321 cccaaattaa tatttaattg atgcattttt ccaattgtct cgttaaaatt tttaatgcca
     1381 tcaaaatcta cagattcttt ggtgaagaca atattaaaaa tgggttgttt ccacatgctg
     1441 tatccagcga ttgcggttaa tataacaaca atgatttgaa taatcatgct taccaatgaa
     1501 cgttttttct taccatatcg cttaccacga tgaatattcg attgttgaaa tgtctcatca
     1561 aatggttcta atcgttgatt tggatttgtc atgcaaggta cctccgtata aagttctttt
     1621 ctttacttat caatatgtct agttgtctgt tataatgatg aaaaatcact gttcaattgt
     1681 agtcttaaat ttgaaagtga aaaagaaaat atcttctaca ttatatactt tttttggcaa
     1741 agaatgtagg ggatagacaa tcattggata cataaaatat ataaaggtag gttaagacat
     1801 tgattagaaa ccgtgttatg aatagcgttg tcaataaata tttgcttcac aatcgctcga
     1861 ttatgtttaa aaatgatcaa gatgttgaaa gattttttta taaaagagaa attgaaaata
     1921 gaaaaaaaca taagcagcct tcaacattaa atgttaaagc aaatttagaa aaattatcat
     1981 tagatgatat gcaagtcttt cgctttaatt tcagacatca aattgataag aaaattttat
     2041 atattcacgg tggattcaat gcactacaac catcaccgtt ccattggaga ttgttggata
     2101 aaatcacttt aagtacatta tatgaggttg tactgcctat ctatccaaag acaccagagt
     2161 ttcatattga cgatactttc caagcgatac aacgtgttta tgatcaatta gtatctgaag
     2221 taggacctca aaatgtcgta gtcatgggtg atggttcagg tggtgcactg gcattatcct
     2281 ttgtacaatc tcttttagat aatcaacagc cattaccgaa taaattgtat ttaatctcac
     2341 caattttaga tgcaacacta tctaataaag atatttcgga cgccttaatc gaacaagatg
     2401 cggttctaag tcagtttggt gtcaatgaga ttatgaaaaa atgggcgaat ggcctaccat
     2461 taacagataa gcgcatatcg ccaattaatg gcacaataga aggattgcca ccagtttata
     2521 tgtttggtgg tggacgtgaa atgacacatc ctgatatgaa gctatttgaa caaatgatgt
     2581 tgcaacatca tcaatacatt gagttttatg attatcctaa gatggtacat gattttccaa
     2641 tttatccaat tagacaatca cataaagcta ttaaacaaat tgccaaatcg atagatgagg
     2701 atgtaacaca aaataactaa taacatataa atgacccagt tacgatttca tgcaagatga
     2761 tatttgacat gaaaacgtaa ttgggttttg tttttgaaaa tatagcaagg gttatataga
     2821 ctaattgtta atgagcttct gagtgaaaat caccaataag tgtattgata tgtgtgatga
     2881 taaagtattg ccctttaatt tgataaaatt gatctaaaaa attatcgaat tgacgtggaa
     2941 tatcactaag ttctgcaaag ttagctgttt tatcataatt gcgatgagca acctcatttt
     3001 gaaatttatc ataatgttct aggaaatgtt gatcttcaag ctgttgttcc acaatagtca
     3061 taatattatc gatggcttgc ttttgtcctt gaacataagc catatgcacg ccacgcttaa
     3121 gtagctgatt tgctggtccg ttaatgtata accctttttg tgcatttaat tctgttaatt
     3181 caaacttaac atcatctaat aaatctagta attcgatatg aatcttttgt tgtaaagcat
     3241 ctgtcatttt aatgctcctt tgtgtggaat tgctatttta tgtcctaatt gttcaggcca
     3301 agtgatagtt ggttgatttt tataatagtg tgctatttgt gttgaaaatg attcaggaaa
     3361 agcatcagaa cgacgtgtgt gctgattaat tcccatcatc attacttcat ttgttgatgc
     3421 tagtgtacca tcttctttag ttaatgttaa aaataaatgc aaccgcttat aatcgtaatc
     3481 ataaatatat aaagtaacag taaatatatc gccaagagac aattctgaga ggtatgtcgt
     3541 atgttcttct agtgtaaata gcgtatatgc taaattttcg cgttctttta aagaaagacc
     3601 gtggctgtaa ttaaaacgat tcacgacatc actaaaaatg atattataat ttgcatcatg
     3661 catatgattg ttatggtcaa tgaattcaga tgttacggtt tgagtatgtg taaaaagttg
     3721 tttgataata accatctcct tagtgactat catactaaaa taaattaaga catacacgtt
     3781 atttaattca tgataaaata atagagaatt cgttatttag ggggagtatc gatggcttta
     3841 aagatacggt atcaaactac atatgaaccg tttaaagtag ttgatgatat caaagagata
     3901 ccaaaagatg cgactattgt ttggtatgat tttgatgaac cgaatgaaca agaaaatgaa
     3961 tggtttaaag cacattttaa ttttaatgat ttagaagtgg atgatgcaat taacggtatg
     4021 ccacgagcta aatataaatc ttataaagat tatcaatact tagtattcca tagtattatg
     4081 gggtcaaact attcaccaat agcactaaat atttttatac aagacaatgt attagtaaca
     4141 tatcatcatc aaacgttgga atcattaaat aaagtagtct ataagtatat gaatacatta
     4201 gatgcagaat tagattgtgc agatgtcgtc atattaattt tagatatgat ggttgataaa
     4261 tatttcaact ttgtatatgc acttgaagat agtgtttatc attttgagga ccgccatgta
     4321 gatgatcgct ttaataaaat ggtcatggat agtgttttta agttacgttc ggatttgatt
     4381 aaagtaaaac gtgtactgtt tcctatgcaa gagctaattg atacaatgaa acaaaatggt
     4441 gatttgatta tagataataa acattcgcta tacattcaac atatcgatga ccacttgatt
     4501 aagcaacgta atatcattcg tactgctcaa gaaatgacaa acgaaatacg agaaaatttt
     4561 gagtcataca cgtcatttag aatgaatagt attatgcaag tattaacgtt agtatcagtt
     4621 atattttcac cgttaacatt catagctggg atttatggta tgaactttgt aaatatgccg
     4681 gcattacatt tacattatgg atattatatt tgcttagctg taatgtttgt tatagctgtc
     4741 gtattaatca tctttttcag acgtaaaaaa tggttttaaa ttagaaatga cgaaaaggta
     4801 ggtttaatca tgagtgattt tcaaagagaa cagagaaaaa atgaacatgt tgaaatagca
     4861 atggcccaat ctgacgcaat gcattcagat tttgataaga tgcgttttgt gcatcattct
     4921 atcccatcaa ttaatgtaaa tgatatcgat ttgacatcac agacgcctga cttaacgatg
     4981 gcatatccgg tttatattaa tgcaatgacg ggtggtagcg agtggacgaa aaacatcaat
     5041 gaaaagctag ctgtagttgc aagagaaact ggcttagcga tggcagttgg atcaacacat
     5101 gcggcattga gaaatccacg catggctgag acgtttacga ttgcgcgaaa aatgaatcct
     5161 gaaggcatga tttttagcaa tgttggtgcg gacgtaccag tagaaaaggc tttggaagca
     5221 gttgaattac ttgaggcaca agcgttacaa atccatgtta attctcctca agaattagtt
     5281 atgcctgaag ggaatcgtga atttgtgact tggttagata atatagcgtc gattgtatca
     5341 cgagtgtctg ttccagtcat tataaaagaa gttggatttg gtatgagcaa agaattaatg
     5401 catgacttac aacaaatagg cgtcaagtat gtcgatgtta gtggcaaagg tggtactaac
     5461 tttgtagata ttgaaaatga acgtcgtgca aataaagata tggattactt atcatcatgg
     5521 ggacagtcta cagttgagtc attacttgaa acaacggctt atcaaagcga aatttcagtt
     5581 ttcgcgagtg gtggtttacg tacaccactc gatgcaatta aaagtttagc acttggtgca
     5641 aaggcaactg gtatgtcacg tccgttttta aatcaagttg aaaataatgg cattgcacat
     5701 acagtagctt atgtagaatc atttattgaa cacatgaaat caataatgac gatgttagat
     5761 gctaaaaata ttgacgactt aacacaaaaa caaatcgtat ttagtccgga aatattgtca
     5821 tggatagaac aacgtaactt gaatatacat cgaggataaa taaaagaggg ggcgaaggcc
     5881 tcctctttta tttatgagtt tggtgtttaa ctaaaccatt gaatgaatcc cataatgact
     5941 ataacaccaa ttaaatcgat taagaatgca ccaacaatag gtacaactaa atatgcttta
     6001 ggtgagtttc catatttttt agtaataaca tctaaatttg ccatggcatt tggcgttgca
     6061 ccaagcccat gcccgataaa accacctacc attactgcag catcataatc ttttcctaaa
     6121 cctctaaata aaattaaaac agcaaataaa atcataacga caacttgaac taaaacgata
     6181 ataataagag gtatagcaag tttataaatt tcgattaatt gaatgctcat tagcgcaaga
     6241 gataagaaaa tacctaatgc gacatcgcca atttgattag taatttttaa atctacaata
     6301 ttaaaattaa aactttcaga gatatttcgg acaataacag ctacaaataa tgagccaacg
     6361 taaattggaa cattaatccc tgtttgagct gtaaacaaat gactgaaata acttccaact
     6421 gccatacaga atacaacgat tgtaaattga atgaagaata cttcagtggc attaaattta
     6481 ctatgtaaat gttcgttata tgctacttgg ctataatctt taaatgtatc gtcactatgt
     6541 tgaggcttta agttataacg tttgattaaa aattttacaa caggaccacc gataagccct
     6601 ccaaatacaa gacctaaagt tgcagctgca agagccgctg tcagtgccga atcaatacct
     6661 aaatcttgaa ttgtcttacc ataagcagca gcattaccat gaccgccttc catagacatg
     6721 gaacctgctg ttaatcctaa caaaggttta atatttaata cttttgctag tgatacacca
     6781 actatgtttt gaatgactga aatgatagca caaaacataa agtataatag caagactttg
     6841 ccacctaatt taaataattt caatgatgca ccaagaccga ttgtcgtaaa gaatgctaac
     6901 atgaagaaat cttgaatgaa tgaagcatct aatttaatct taaccatgcc aaatgaatcc
     6961 aaagtcgcaa ctaaaatagc aaagattaag ccgccaatca caggtgctgg tatacaaatg
     7021 cgttttaaaa aattaacgtg attaacgata gctttaccaa gtaagtaaag tatacaggct
     7081 aaacataatg ttgtaattgc attaagttct atcattttca aatccccctt gttgaatatc
     7141 cctctcccta aataagcttt tatatgtaag cgttataaaa gggcactttt aacattatac
     7201 atgcaacaaa tcaatatttc tagtttatga aaataatatc aataatatta atgcatctaa
     7261 atttaataat tataaataat tctattgtta tattttataa ttaagagcaa gttcaaaaag
     7321 aggagatgaa tcaaaaaata tgatggacac aaagaaaaac aatcaaccca tccgcatgtt
     7381 gacggcacga ttgattgact tataaggaga tagatttcat tttcatgata gtaatcaatg
     7441 aaagatggca tttatttcca agtatcttcg ggaaacatac aatctgattt acgcatgcga
     7501 gacacaaatg gattaccttt cactgtgtaa cgtaaagatt tatgtgtcca atcaccttta
     7561 tttggaatac cgattcgtgg actagcaata atatctttag gatatttacg attcttagta
     7621 tcaatagaca atctacagtc atttaacgta gcgccatcga tagcccgtgg aatgttaaat
     7681 gccttagtcc attttcctgg gccattcgtt acctcgtagc ctttcttacc tctgttacgg
     7741 aacatagcgg ataaaccttc ttctggttca attgcgcgta taagtacgcc ttcaggtata
     7801 ccttcagatt ttgttacaaa attaatgagt aaatgcgtat gcatgacatg tgcataaatt
     7861 gtaccaccac gtttatataa tgacgtgact ttaggtgtta ttttaccgcc atagccatga
     7921 gccgcacgat cattcaaacc taagtaagct tccgtttcca cgatgtagcc tgtatacgtt
     7981 tgagtggtat cctgataaat cactttgaca cctaataagt ttttagcaat ttgtcttgta
     8041 tcattattaa cgaaatccat aatattctcc tcgcaattat atgattaaat taaataaaaa
     8101 tgaaaaagat atttttattg tggtaaatgt ttgataaaat agtattgtta agaattataa
     8161 aaatgtaaag tatatacttt gttatggaat attttatttg cagagattga gactctttta
     8221 aacgtgaaca ttgcagtgtg acatattttt agtatagtta acgaagcgtt gactttcaaa
     8281 tgagtctcca tatagatgtg tacttgtagc gggttttcat tttactagtg atgaaatata
     8341 aagtccattt tccaaagtga atgacgattg aaatgaagtg gcttgataat gaatcttgta
     8401 aattataacg gtaataataa ttaaaaatat aaatatgatt aacgaaaggg agattacaat
     8461 ggaacaatta cctcaagtag gttttatgaa atcattcgta ttattttgga aaaattatgt
     8521 aaattttaag gggagatcaa gacgtagtga atattggtat atggcactat ggcatttgat
     8581 acttatggtt ccagctgtgt gtgtgctaat aataaaccta tttctattct ttatttctat
     8641 agcggcagat aacgaaacat taatagttat gagtatattg atgattatcc ttacatctgt
     8701 gtatactttt gtctatagtt tggcaacgtt tattccaaat ttagcgttgt gcgttcgaag
     8761 atttcacgat atatcaagaa caatgttatt tccaatgatt atgacagtat tcagtattat
     8821 attttatata gtattacaga ttatcgatag ttattatgac agtgatttta cattaatgcc
     8881 gatgggtctt aatattttgt tagtacttct ttattttgtt tatttaggcc taacaattgt
     8941 tatgatagta ttcctatgct ttgacagtaa gccggcaaat aaatatggcg aaagcccaaa
     9001 atacccgtct acaattaaaa atcaaccagt gacatctgaa acaccggaaa aagcgtgtac
     9061 atctgaaaca ccgaaaacac cggaagaaac agataatcaa ttgtaatgat ataaatatct
     9121 cggcacaatg atgctgggat atttttttac aaataaggca ataaaggcat ggaaaaaaca
     9181 tatctagtgc taatgatact taacatgcaa aaatggcact atttaaattt ttcaatttca
     9241 gaaattttaa aaataaattc attttaaaaa tattgcattt atagagaatg gaaatgttat
     9301 attaagataa atacatattt ttttaggagg caaagtatgg gacttgtaat tgaacatgtt
     9361 acgaaacgtt ttggcaagat gacggcagta aatgatattt cattagagtt agaatcaggc
     9421 aaaatgttag gttttctagg aagaaatggt gcaggtaaaa caacgacatt tcgtatgatt
     9481 ttaggcttaa gtgaaccaac tgaaggacac attacgtata acggtaaaaa actagataaa
     9541 acaatgtaca atcgtattgg ttatttaccg gaagaacgcg gtttacatgg gaaattgaca
     9601 gttgaagaag aattgaaata tttggcaact ttaaagggaa tgtcaaaaac agaaatacaa
     9661 cagcaaatat cgtattggct tgagcgtttt gatattactg aaaaccgcaa aaaacgaatt
     9721 gatagtctat caaaagggaa tcaacaaaaa attcagctgt tagcaagtat gttacataaa
     9781 ccagaattac taatattaga tgagcctttc agtggtctag acccagttaa tgttgagtta
     9841 ttaaaagaag cagttaaaga cttaaatgat tggggcagta caatcgttta tagttctcac
     9901 cgtatggagc atgttgaaga attatgtgat gatgtttgta ttttagataa aggtcaactt
     9961 gttgtttctg gtgatatcaa tcatgtacgt gcatctaatg gtaacaagaa agttgtcatt
    10021 gaatcagaaa caacactacc agatttaaca aacattagag gcatcattca tagtgaaaac
    10081 atgaagcaag gtcttcagtt gacgattgag aatgaagacg tagccaaaga tatttatcaa
    10141 gttgtggcac atcaaggtta tgttaaacga ttccaagttg tagaaccatc attacaagat
    10201 atattcattg agaaggttgg tggcaaggat gcataagttt tgggcgacat ttactttgac
    10261 atatatcaaa aaaattaaag ctaaatcatt tgtaattttt atgattatta ttgctgcctt
    10321 aatgattggc ctatctaaca ttgataagat tattaacatg tttgatgatg gtccagataa
    10381 aattggtgta gcagcaccga atgaacaaat ctataaggtt ttcaagcaac aggcgaatac
    10441 atttcatagt gatgctaagt ttactaaagt ttctatagaa gatgctgaaa aagaagtaaa
    10501 aaaacataaa cttgataaag cttatattat caaggtgaat caaaatcgca cgttacaagg
    10561 gacaatcatt agtgagaagc gagtaagtca tgaagattca caaaaagtac aagcactgct
    10621 tacagctatt caaacaaata tggtagccgg ggaattaaat atcaacaaag aagacttaca
    10681 aaaattacaa gcacaaagta aagttgataa taaggtgatt tctaatgatg aagtcgataa
    10741 agtatcagaa ggacaaaaga ttttcaacta tgctttagct tatggcatta tctttctaat
    10801 gttcttcatt gttttaaatt atgcatctca aatagctatg gaaattgcta gtgagaaaac
    10861 atcgcgtgtc attgaaatga ttattacaag tatttcacca atccaacata tttttgctaa
    10921 aattttaggc gttatagcag ttgccgtgac tcagattatt ttaattgtat tgatggcagt
    10981 tatatgcatt tttgcctttg acttaaaaga cttgctacaa ggttttaacg tggaaatgaa
    11041 tcaattgtct tggcaaatta ttattgtcgg aattataagt agtattgttg gaattatggc
    11101 gtatgtatta cttgcagcta tcctcggatc attaacatct agaatagaag atttaaatca
    11161 atctttgatg ccgctgacat tacttggtat gattgcattt tatatcgcga tattcacgat
    11221 aaataatcct gatgggcgac ttgctttaat cacaagttat attccgtttt tagcgccatt
    11281 tcaattagtt gtacgcgctc aaacttcagg gcttcaaata catgaagtca tcttaagttc
    11341 attaatctca attgtaatgg tagctgtctt gatatggatt gcaattaaaa catataaaga
    11401 ttcagtactt acatttgaac gtggcttatt taattcaatg aaacgtgtat ttaaaaaata
    11461 aaattgatga gagtgacaca catttatc
//
LOCUS       DAHHYJ010000084        11401 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000084 DAHHYJ010000000
VERSION     DAHHYJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.084"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..433
                     /locus_tag="NBK22_001716"
     CDS             <1..433
                     /locus_tag="NBK22_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCD2414901.1"
                     /translation="GSDKDKYNEEVNPDIDVFVVREGNGRQADNHSIGGVTKTNRQSY
                     FDQINTPILEIKQGSEDLYKGYHLIFKEDISLKELDYRLRERAIKQHGLYSNGLKQGR
                     ITITMRDGKSHTIDLSQKLEKERMGDSIDGRQIQKILVEIK"
     gene            718..1413
                     /locus_tag="NBK22_001717"
     CDS             718..1413
                     /locus_tag="NBK22_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCD2414902.1"
                     /translation="MKMKNIAKISLLLGILATGVSTTTEKPVHAEKKPIVISENSKKL
                     KAYYTQPSIEYKNVTGYISFIQPSIKFMNIIDGNSVNNLALIGKDKQHYHTGVHRNLN
                     IFYVNEDKRFEGAKYSIGGITSANDKAVDLIAEARVIKADHIGEYDYDFFPFKIDKEA
                     MSLKEIDFKLRKYLIDNYGLYGEMSTGKITVKKKYYGKYTFELDKKLQEDRMSDVINV
                     TDIDRIEIKVRKA"
     gene            1702..2772
                     /locus_tag="NBK22_001718"
     CDS             1702..2772
                     /locus_tag="NBK22_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCD2414903.1"
                     /translation="MKMRTIAKTSLALGLLTTGAITVTTQSVKAEKVQSTKVDKISTL
                     KAERLAMINITAGANTVTTQAAKTGQERTPKLEKAPNTNEEKTSTSKIEKVSQPKQEA
                     QKLLNISATPAPKQEQSQTTTESTTPKTRVTTPPSTNTPQPMQSTKSDTPQSPNIKQA
                     QTDMTPKYEDLRAYYTKPSFEFEKQFGFMLKPWTTVRFMNVIPNRFIYKIALVGKDEK
                     KYKDGPYDNIDVFIVLEDNKYQLKKYSVGGITKTNSKKVDHKAELSITKKDNQGMISR
                     DVSEYMITKEEISLKELDFKLRKQLIEKHNLYGNMGSGTIVIKMKNGGKYTFELHKKL
                     QEHRMADVIEGTNIDKIEVNIK"
     gene            2891..3028
                     /locus_tag="NBK22_001719"
     CDS             2891..3028
                     /locus_tag="NBK22_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414904.1"
                     /translation="MFAKVAFLCLKSDGTSNVPSVESHQNHFYLTNIMDFLIYLTMIQ
                     I"
     gene            3137..3982
                     /locus_tag="NBK22_001720"
     CDS             3137..3982
                     /locus_tag="NBK22_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL4"
                     /protein_id="HCD2414905.1"
                     /translation="MKITTIAKTSLALGLLTTCVITTTTQAAKASTPSSTKIEAPQQV
                     ANATTPSSTKVEAAQSKPNAKTPPSSNVDTSPLQSPTTKQVPTEINPKFKDLRAYYTK
                     PSLEFKNEIGIILKKWTTIRFMNIVPDYFIYKIALVGKDDKKYGEGVHRNVDVFVVLE
                     ENNYNLEKYSVGGITKSNSKKVDHKAGVRITKEDNKGIISHDVSEFKITKEQISLKEL
                     DFKLRKQLIEKNNLYGNVGSGKIVIKMKNGGKYTFELHKKLQENRMADVIDGTNIDNI
                     EVNIK"
     gene            4346..5050
                     /locus_tag="NBK22_001721"
                     /pseudo
     CDS             4346..5050
                     /locus_tag="NBK22_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
     gene            5496..6191
                     /locus_tag="NBK22_001722"
     CDS             5496..6191
                     /locus_tag="NBK22_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCD2414906.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVHAKEKQERVQHLYDIK
                     DLYRYYSSESFEFSNISGKVENYNGSNVVRFNQEKQNHQLFLLGKDKDKYKKGLEGQN
                     VFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFLINKEEV
                     SLKELDFKIRKQLVEKYGLYKGTTKYGKITINLKDEKKEVIDLGDKLQFERMGDVLNS
                     KDIQNIAVTINQI"
     gene            6530..7228
                     /locus_tag="NBK22_001723"
     CDS             6530..7228
                     /locus_tag="NBK22_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL8"
                     /protein_id="HCD2414907.1"
                     /translation="MKFTAIAKAIFVLGILTTSAMITENQSVNAKGKYEKMNRLYDTN
                     KLHQYYSGPSYELTNVSGQSQGYYDSNVLLFNQQNQKFQVFLLGKDENKYKEKTHGLD
                     VFAVPELVDLDGRIFSVSGVTKKNVKSIFESLRTPNLLVKKIDDKDGFSIDEFFFIQK
                     EEVSLKELDFKIRKLLIKKYKLYEGAADKGRIVINMKDENKYEIDLSDKLDFERMADV
                     INSEQIKNIEVNLK"
     gene            7608..8306
                     /locus_tag="NBK22_001724"
     CDS             7608..8306
                     /locus_tag="NBK22_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCD2414908.1"
                     /translation="MKFTELAKATLALGILTTGVFTAESKAVHAKVELDETQRKYYIN
                     MLHQYYSEESFESTNISVKSEDYYGSNVLNFNQRNKTFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKHGFSINELFFIQK
                     EEVSLKELDFKIRKMLVEKYSLYKGASDKGRIVINMKDEKKYVIDLSEKLSFDRMFDV
                     MDSKQIKNIEVNLN"
     gene            8672..9355
                     /locus_tag="NBK22_001725"
     CDS             8672..9355
                     /locus_tag="NBK22_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCD2414909.1"
                     /translation="MKFTALAKATLALGILTTGTLTTEVHSGHAKQNQKSVNKHDKEA
                     LYRYYTGKTMEMKNISALKHGKNNLRFKFRGIKIQVLLPGNDKSKFQQRSYEGLDVFF
                     VQEKRDKHDIFYTVGGVIQNNKTSGVVSAPILNISKEKGEDAFVKGYPYYIKKEKITL
                     KELDYKLRKHLIEKYGLYKTISKDGRVKISLKDGSFYNLDLRSKLKFKYMGEVIESKQ
                     IKDIEVNLK"
     gene            complement(9440..9541)
                     /locus_tag="NBK22_001726"
     CDS             complement(9440..9541)
                     /locus_tag="NBK22_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414910.1"
                     /translation="MENFDVGGGNFTFMEIGIGFMTHKPKRLNDDID"
     gene            9619..11175
                     /locus_tag="NBK22_001727"
     CDS             9619..11175
                     /locus_tag="NBK22_001727"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD2414911.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEVEQEINAY
                     LKELGVLKDE"
     gene            11168..>11401
                     /locus_tag="NBK22_001728"
     CDS             11168..>11401
                     /locus_tag="NBK22_001728"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD2414912.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKQFADFTKINQGLQIAINERK
                     TEYSPELYFYITNEFLRPNSQTKYFIENPPQSVI"
BASE COUNT         4545 a         1570 c         2031 g         3255 t
ORIGIN      
        1 tggttctgat aaagataaat ataatgaaga agttaaccca gatatagatg tgtttgtcgt
       61 tagagaagga aacggtagac aagctgataa tcattcgatt ggtggcgtaa caaaaactaa
      121 tagacaatca tattttgatc aaattaatac accaatttta gagattaaac aaggtagtga
      181 agatctttat aaaggttatc atttaatatt taaagaagac atctcattaa aagaacttga
      241 ttatagatta cgagaacgtg caattaagca acatggcttg tattcaaatg gtcttaaaca
      301 aggtcgaatt acaattacaa tgagagatgg caaatcacat actatcgatt taagtcaaaa
      361 acttgaaaaa gaacgtatgg gtgattctat cgacggcaga caaatacaaa aaattctagt
      421 agaaatcaaa taatactttc taacaacaaa gcgctatgtt gaatagtgct tgttatggaa
      481 actatggaag ttaagcgacg tactgttgct tagcttcttt ttttgagggt caaagttaca
      541 aaactcacac aaacagtcgc accacgcatt atcttttgct taaatagctt aatcatattt
      601 tatgaatagt taaaaacagg ttaatgtgaa tatccgagta cagctcctat aatatgggtg
      661 tatgattcaa attacgtaat aaaacaatct aattataata gattggagca tacaactatg
      721 aaaatgaaaa atattgcaaa aataagtttg ttattaggaa tattagcaac aggtgtaagc
      781 actacaacgg aaaaaccagt tcatgccgaa aagaaaccta ttgtaataag tgaaaatagc
      841 aaaaaattaa aagcttatta tactcaaccg agtattgaat ataaaaatgt gacaggttat
      901 atcagtttca ttcaaccaag tattaaattt atgaatatca tagatggtaa ttctgttaat
      961 aatcttgctt taattggcaa agataagcaa cattatcata cgggtgtaca tcgtaatctt
     1021 aatatatttt acgttaatga ggataagaga tttgaaggtg caaagtactc cattggcggt
     1081 atcacgagtg caaacgataa agctgtcgac ctaatagcag aagcaagagt tattaaagca
     1141 gatcatattg gtgaatatga ttatgacttt ttcccattta aaatagataa agaagcgatg
     1201 tcattgaaag agattgattt taaattaaga aaatacctta ttgataatta tggtctttac
     1261 ggtgaaatga gtacaggaaa aattaccgtc aaaaagaaat actatggaaa gtatacattt
     1321 gaattggata aaaagttaca agaagaccgt atgtccgatg ttatcaatgt cacagatatt
     1381 gatagaattg aaatcaaagt tagaaaagca taacacaaac ttgacgacga aataatttga
     1441 aattgaaata gagaggttaa gtgatgatca aacgttgctt aacttctttt taatgcttaa
     1501 aaatcatttc aaaggcacat agaaacgcta tattaatctc ataatcactc attatttttt
     1561 gcttaaatta cttaataata cttcaataat tgttaaaagg ggtttaatgt gattatctta
     1621 gaacgccatc tataatgatg ttgtatgatt caaattacgt aaaaagacaa tcgaatataa
     1681 tatagattgg agcatacaat tatgaaaatg agaacaattg ctaaaaccag tttagcacta
     1741 gggcttttaa caacaggcgc aattacagta acgacgcaat cggtcaaagc agaaaaagta
     1801 caatcaacta aagttgacaa aatatcaacg cttaaagcag agcgattagc aatgataaac
     1861 ataacagcag gtgcaaatac agtaacaaca caagcagcta aaacaggaca agaacgcacg
     1921 cctaaactcg aaaaggcacc aaatactaat gaggaaaaaa cttcaacttc caaaatagaa
     1981 aaagtatcac aacctaaaca agaagcgcag aaattgctta atatatcagc aacgccagcg
     2041 cctaaacaag aacaatcaca aacgacaacc gaatccacaa cgccgaaaac tagagtgaca
     2101 acacctccat caacaaacac gccacaacca atgcaatcta ctaaatcaga cacaccacaa
     2161 tctccaaaca taaaacaagc acaaacagat atgactccta aatatgaaga tttaagagcg
     2221 tattatacaa aaccgagttt tgaatttgaa aagcagtttg gatttatgct caaaccatgg
     2281 acgacggtta ggtttatgaa tgttattcca aataggttca tctataaaat agctttagtt
     2341 ggaaaagatg agaaaaaata taaagatgga ccttacgata atatcgatgt atttatcgtt
     2401 ttagaagaca ataaatatca attgaaaaaa tattctgtcg gtggcatcac gaagactaat
     2461 agtaaaaaag ttgatcacaa agcagaatta agcattacta aaaaagataa tcaaggtatg
     2521 atttcacgcg atgtttcaga atacatgatt actaaggaag agatttcctt gaaagagctt
     2581 gattttaaat tgagaaaaca acttattgaa aaacataatc tttacggtaa catgggttca
     2641 ggaacaatcg ttattaaaat gaaaaacggt gggaagtata cgtttgaatt acacaaaaaa
     2701 ctgcaagagc atcgtatggc agatgtcata gaaggtacaa acattgataa aattgaagtg
     2761 aatataaaat aatcatgacg ttctctaaat agaagctgac atcggaaaaa caagaagtta
     2821 agtgacaacg gtttacatgt tgcttagctt cttttattat gcgcaatgat gtaaaaagac
     2881 gaatattcat ttgtttgcaa aagtggcatt tctatgtctt aaaagtgacg gaacctcaaa
     2941 tgtgccaagt gttgaatcac atcaaaatca tttttattta acgaacatta tggatttctt
     3001 aatttactta acgatgattc aaatatagtt aaacaaggtt taatgtgaat ggagcaatac
     3061 gccatctata ataaagctgt atgattcaat gaatgtaatc gaacaaatct aataattacg
     3121 aatggagcat acaactatga aaataacaac gattgctaaa acaagtttag cactaggcct
     3181 tttaacaaca tgtgtaatca caacgacaac gcaagcagca aaagcgtcaa caccatcctc
     3241 aactaaaata gaagcaccgc aacaagtagc aaatgcaaca acaccatctt caactaaagt
     3301 agaagcagcg caatcaaaac caaacgcgaa aacaccacct tcgtctaatg tagacacatc
     3361 accactacaa tcgccaacca caaaacaagt accaacagaa ataaatccta aatttaaaga
     3421 tttaagagcg tattatacga aaccaagttt agaatttaaa aatgagattg gtattatttt
     3481 aaaaaaatgg acgacaataa gatttatgaa tattgttcca gattatttca tatataaaat
     3541 tgctttagtt ggtaaagacg ataaaaaata tggtgaagga gtacatagga atgtcgatgt
     3601 atttgtcgtt ttagaagaaa ataattacaa tctggaaaaa tattctgtcg gtggtatcac
     3661 aaagagtaat agtaaaaaag ttgatcacaa agcaggagta agaattacta aggaagataa
     3721 taaaggtata atctctcatg atgtttcaga attcaagatt actaaagagc agatttcctt
     3781 gaaagaactt gattttaaat tgagaaaaca acttattgaa aaaaataatc tgtacggtaa
     3841 cgttggttca ggtaaaattg ttattaaaat gaaaaacggt ggaaagtaca cgtttgaatt
     3901 gcacaaaaaa ttacaagaaa atcgcatggc agatgtcata gatggcacta atattgataa
     3961 cattgaagtg aatataaaat aatcatgaca ttctctaaat agaagctgtc atcggaaaaa
     4021 caagaagtta agtgacaacg gcctacatgt tgcttagctt cttttgttat gttcgatgat
     4081 ttgagaaccc gaattttcga tgggtccaaa tatgacgtgg aagagtcctg aatttatctg
     4141 taaatcccta tctatcgggt gtgaagcaca acgggatcag ttttatttaa cgcacattat
     4201 agattcctta atttacttaa taatgattca atgattatta aagatggttt aatgtgaaag
     4261 gtcaaatacg ccaactataa taaagctgta tgattcaata gacgtaagcg aacaaatcta
     4321 ataattacga atggagcata caactatgaa aatgacagca attgcgaaag caagtttagc
     4381 actaggtatt ttagcaacag gaacaataac atcaacgcat caaactgtaa atgcgagtga
     4441 acatgaagca aaatatgaaa atgtgacaaa agatattttt gacttaagag attactatag
     4501 tggcgcaagt aaggaactta aaaatgttac tggttatcgt tatagcaaag gtggcaagca
     4561 ttaccttatc tttgataaaa atagaaaatt cacaagagta tagatatttg gtaaagatat
     4621 tgaaagattt aaagcacgca aaaatccggg attagatata tttgtagtta aagaagcgga
     4681 aaaccgtaat ggcacagtgt tttcatatgg tggtgtcact aagaaaaatc aagacgctta
     4741 ttatgattat ataaacgcac caagatttca aatcaagaga gatgaaggtg acggtattgc
     4801 tacgtacggt agagtacact acatttataa agaagagatt tcacttaaag aactcgactt
     4861 taaattgaga cagtatttaa ttcaaaattt tgatctgtat aaaaagtttc ctaaagatag
     4921 taagataaaa gtgataatga aagatggcgg ctattatacg tttgaactta ataaaaaatt
     4981 acaaacaaat cgcatgagtg acgtcattga cggtagaaat attgaaaaaa tagaagccaa
     5041 cattagataa tgcaatgaaa tatggataat agtaaaatat ggatagtata gaggagttag
     5101 gcaacataag ttgcttagct tcttttttgt gttggagaga tgaaaaagaa gcgtatcgtt
     5161 gaataataaa aacaccaata aaacttgtgg gaatagttga tacctatagt cgcgcgttgt
     5221 ccttttcgtg acatgaaaca atgtggaaaa cataattaaa ttgagggaaa gtgtgaatag
     5281 ttaaaaaatt agcattgtgt tataaaaaat aattaatact gttaggattt cattaactaa
     5341 cttaacgttg gttcaaaaat agttaaaaag aggttaattc atagcgcagt atctcgctta
     5401 tataatgata gtagattgtt cgtattacgt aattgaatta atcatataaa aatatattaa
     5461 gacaaaattt ataaatagat tgggagaata gtactatgaa attaaaaacg ttagctaaag
     5521 caacattggc attaggctta ttaactactg gtgtgattac atcagaaggc caagcagtcc
     5581 acgcaaaaga aaagcaagag agagtacaac atttatatga tattaaagac ttatatcgat
     5641 actactcatc agaaagtttt gaattcagta atattagtgg taaggttgaa aactataacg
     5701 gttctaacgt tgtacgcttt aaccaagaaa aacaaaatca ccaattattc ttattaggaa
     5761 aagataaaga taaatataaa aaaggccttg aaggccagaa tgtctttgtg gtaaaagaat
     5821 taattgatcc aaacggtaga ctatctactg ttggtggtgt gactaagaaa aataacaaat
     5881 cttctgaaac taatacacat ttatttgtta ataaagtgta tggcggaaat ttagatgcat
     5941 caattgactc atttttaatt aataaagaag aagtttcact gaaagaactt gatttcaaaa
     6001 ttagaaagca attagttgaa aaatatggtt tatataaagg tacgactaaa tacggtaaga
     6061 tcactatcaa tttgaaagac gagaaaaagg aagtaattga tttaggtgat aaactgcaat
     6121 tcgagcgcat gggtgatgtg ttgaatagta aggatattca aaatatagca gtgactatta
     6181 atcaaattta aattaaacaa tcaatgactt taaagtaata aatttgaagc agcttaacga
     6241 tgaaatgttg aataaatacg tacatcctcc aaaaaggggc gtatctaaat caacagtgtc
     6301 gttaggctgt ttttatgttt tataacgcag ggtatgagca tactaaaaat tcaaattact
     6361 cctgaaagtg atgtccattg aatattaatt agaacttcat taaccatgat ttaagtttaa
     6421 ttaaacgagt gttaatgtca gcctgtctca atgcccttta taataaatgt gtattattca
     6481 aattacgtaa taaaagcaat ccaatatatt aagattggag cacatgaata tgaaatttac
     6541 agcgatagct aaagcgatat ttgtattagg aatattaaca acaagtgcaa tgataacaga
     6601 aaatcaatcg gttaatgcaa aaggaaagta tgaaaaaatg aaccgtttat atgatacaaa
     6661 caagttacat caatactatt caggacctag ttatgagtta acaaatgtta gtggtcaaag
     6721 tcaaggttat tatgactcta acgttttgct ttttaaccaa caaaatcaaa agttccaagt
     6781 gtttttattg ggaaaagatg aaaataaata caaagaaaaa acgcatggcc tcgatgtctt
     6841 tgcggtacca gaattagtag atttagatgg aagaatattt agtgttagtg gtgtaacaaa
     6901 gaaaaacgta aaatcaatat ttgagtctct aagaacgcca aacttacttg ttaaaaaaat
     6961 agacgataaa gacggttttt ctattgatga atttttcttt attcaaaagg aagaagtgtc
     7021 attgaaggaa cttgatttta aaataagaaa actgttaatt aaaaaataca aattgtatga
     7081 aggggcagcc gataaaggta gaattgttat taatatgaaa gatgaaaata agtatgaaat
     7141 tgatttaagt gataaattag atttcgagcg tatggcagat gtcattaata gtgaacaaat
     7201 taaaaacatc gaagtgaatt tgaaataatc aatgatatat atagaatgaa agcttaagaa
     7261 gcggtttaat aatcccatgt ttaatgattt tgatacgtgt ttttataata aaaacatatc
     7321 gaacattgac tacgttatta agctgctttt ttgtacactt tgtaacgaat aacttaagat
     7381 ttaaaactaa tcggaaagaa caatgattcc cccaaaaaaa atttatgttg ctattaaaaa
     7441 tcagttaata cgaatgttaa aatacgtttg attttcatta ataatgattc aagtttattt
     7501 aaatgagcgt taatgtcagt ctgttttgat gcaccttata ataaagacag atagttcaaa
     7561 ttacgtaatc ataacaatcc aatatatcaa gattggagca aataaatatg aaatttacag
     7621 aattagcaaa agcaacatta gcattaggaa tattaactac aggtgtgttt acagcagaaa
     7681 gtaaagctgt tcacgcgaaa gtagaacttg atgagacaca acgcaaatat tatatcaata
     7741 tgctacatca atactattct gaagaaagtt ttgaatcaac aaacattagc gttaaaagtg
     7801 aagattacta tggttctaac gttttaaact ttaaccaacg aaataaaact ttcaaagtgt
     7861 ttctgcttgg tgacgataaa aataaatata aagaaaaaac acatggcctt gatgtctttg
     7921 cagtacctga attaatagat ataaaaggtg gcatatatag cgttggcggt ataacaaaga
     7981 aaaatgtgag atcagtgttt ggatttgtaa gtaatccaag tctacaagtt aaaaaagttg
     8041 atgctaaaca tggcttttcg ataaatgagt tgttctttat tcaaaaggaa gaagtatcgt
     8101 tgaaggaact ggattttaaa ataagaaaaa tgttagtcga aaaatatagt ttgtataaag
     8161 gcgcgtcaga taaaggtaga atcgttatta atatgaaaga cgaaaagaaa tatgtaattg
     8221 atttaagtga aaaattaagt tttgatcgta tgtttgatgt aatggatagt aagcaaatta
     8281 aaaatattga agtgaatttg aattagtttg agttaatagc ataatagctt aagaagcgac
     8341 ttaacgacaa aatgtgaatt gacatctgtg tccttatata aggaactgtg ttaaatacat
     8401 tactgttgtt aagttgtttt tgtaattcaa agagcagaac agagtaacat catcagttgt
     8461 agtaaacgat aatccagtaa aacaactaaa tgaaataatg aaagtcattt aacctgaaca
     8521 ttaaaatata tttgtttttc attaagaata attcaagtat atttaaatcg aggttaatta
     8581 tcgtatgaaa cgatgcacgt tataataaaa atgtatgatt caaattacgt aatgaaaaca
     8641 atccaatata ttaagattgg agcaaataaa tatgaaattt acagcattag caaaagcaac
     8701 attagcttta ggaattttaa caacaggaac tttaacaaca gaagttcatt caggtcatgc
     8761 aaaacaaaat caaaagtcag taaataaaca tgacaaggag gcactatacc gatactacac
     8821 tggaaagact atggaaatga aaaatattag tgctttgaaa catggtaaaa acaacttacg
     8881 ttttaagttt agaggtatta agattcaagt tttactgcct ggaaatgata aaagtaaatt
     8941 tcaacagcgt agttatgagg ggttagatgt tttctttgtt caagaaaaaa gagataagca
     9001 cgatatattt tatactgttg gtggtgtaat acagaataat aaaacatctg gagttgtcag
     9061 tgcaccaata ttaaatattt caaaagaaaa gggtgaagat gcttttgtga aaggttaccc
     9121 ttattacatt aaaaaagaaa aaataacatt aaaagagctg gattataagt tgagaaagca
     9181 tctaattgaa aaatacggac tttataaaac aatctcaaaa gatggtaggg tcaaaattag
     9241 cttgaaagat ggcagttttt ataaccttga tttaagatct aaattaaaat ttaaatatat
     9301 gggggaagtc atagaaagca aacaaattaa agatattgaa gttaacttaa agtaaatcat
     9361 tacgaataat taaaagtaat tgaagcggct taacggtgaa atgtaaattg gtgcgcatag
     9421 cttatacaaa aaggatgcat caatcgatat cgtcgttaag ccgttttggt ttgtgtgtca
     9481 tgaatcctat cccaatctcc ataaaggtaa aatttccacc accaacatca aaattctcca
     9541 catcgcaaca taaccaaatg ttataataaa tctattacac aaagagataa attacttatg
     9601 caaaggcgga ggaatcacat gtctattact gaaaaacaac gtcagcaaca agctgaatta
     9661 cataaaaaat tatggtcgat tgcgaatgat ttaagaggga acatggatgc gagtgaattc
     9721 cgtaattaca ttttaggctt gattttctat cgcttcttat ctgaaaaagc cgaacaagaa
     9781 tatgcagatg ccttgtcagg tgaagacatc acgtatcaag aagcatgggc agatgaagaa
     9841 tatcgtgaag acttaaaagc agaattaatt gatcaagtcg gttacttcat tgagccacaa
     9901 gatttattca gtgcgatgat tcgtgaaatt gaaacgcaag atttcgatat agaacatcta
     9961 gcgacggcga ttcgcaaagt tgaaacatct acattaggtg aagaaagtga aaatgacttt
    10021 atcgggctgt tcagcgatat ggatttgagt tcaacgcgac taggtaacaa tgtcaaagaa
    10081 cgtactgctt taatctctaa agtcatggtt aatcttgacg acttaccatt cgttcacagt
    10141 gacatggaaa ttgatatgtt aggtgatgca tatgaattcc taatcgggcg ctttgcggcg
    10201 acagcgggta aaaaagcagg cgagttctat acaccacaac aagtatctaa gatactggcg
    10261 aagattgtca cagacggtaa agataaatta cgtcacgtgt acgacccaac atgtggttcc
    10321 ggttcattgt tgttacgtgt tggtaaagaa acgcaagtgt atcgttattt cggacaagaa
    10381 cgtaacaata ctacatacaa cttagcacgc atgaatatgt tattacatga tgtgcgttat
    10441 gagaacttcg agatccgtaa tgatgacaca ttggaaaatc cagccttttt aggcaataca
    10501 tttgatgcgg ttattgcgaa cccaccatac agtgcgaaat ggacagcaga ttcaaaattt
    10561 gaaaatgatg aacgattcag tggttacggc aagcttgcgc caaaatccaa agcagacttt
    10621 gcctttattc aacacatggt acattaccta gacgatgaag gtaccatggc agtcgtactc
    10681 ccacatggtg tattattccg tggtgccgca gaaggtgtga ttcgtcgcta tttaatagaa
    10741 gaaaagaact acttagaagc cgtgattggg ttaccagcca atattttcta tgggacaagt
    10801 attccaacat gtattttagt atttaaaaaa tgtcgccaac aagacgacaa cgtactattt
    10861 atcgatgcat ccaatgattt tgaaaaagga aaaaatcaaa accatttaag cgatgcccaa
    10921 gtcgaacgta ttattgacac atacaagcgt aaagaaacaa ttgataaata cagttacagt
    10981 gcgacattac aagagattgc cgataacgat tacaacttaa acataccgcg atatgtcgat
    11041 acattcgaag aagaagcgcc aattgattta gatcaagttc aacaagattt gaaaaatatc
    11101 gacaaagaaa tcgctgaagt tgaacaagaa atcaatgcat acctgaaaga acttggggtg
    11161 ttgaaagatg agtaatacac aaaagaaaaa tgtgcctgag ttgagattcc cagggtttga
    11221 aggcgaatgg gaagagaagc aatttgctga ttttactaaa ataaatcaag gattgcagat
    11281 tgctattaat gaacgtaaaa ctgaatattc tccagagttg tatttttata taacaaatga
    11341 atttttaaga ccaaatagtc aaactaaata ttttatcgaa aatccccctc aatcagtaat
    11401 t
//
LOCUS       DAHHYJ010000085        11312 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000085 DAHHYJ010000000
VERSION     DAHHYJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.085"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(50..1660)
                     /locus_tag="NBK22_001729"
     CDS             complement(50..1660)
                     /locus_tag="NBK22_001729"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCD2414913.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(1996..2526)
                     /locus_tag="NBK22_001730"
     CDS             complement(1996..2526)
                     /locus_tag="NBK22_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCD2414914.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIENRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDEMDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEVFEDEEDFND"
     gene            complement(2638..3498)
                     /locus_tag="NBK22_001731"
     CDS             complement(2638..3498)
                     /locus_tag="NBK22_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2414915.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            3922..4725
                     /gene="coaW"
                     /locus_tag="NBK22_001732"
     CDS             3922..4725
                     /gene="coaW"
                     /locus_tag="NBK22_001732"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCD2414916.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNASVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
     gene            complement(4855..5526)
                     /locus_tag="NBK22_001733"
     CDS             complement(4855..5526)
                     /locus_tag="NBK22_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCD2414917.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRINYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(5925..7118)
                     /locus_tag="NBK22_001734"
     CDS             complement(5925..7118)
                     /locus_tag="NBK22_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCD2414918.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKEVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPFVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKQATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAGTQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            complement(7118..8302)
                     /locus_tag="NBK22_001735"
     CDS             complement(7118..8302)
                     /locus_tag="NBK22_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCD2414919.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            8613..9083
                     /locus_tag="NBK22_001736"
     CDS             8613..9083
                     /locus_tag="NBK22_001736"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCD2414920.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIV
                     EATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            complement(9235..9585)
                     /locus_tag="NBK22_001737"
     CDS             complement(9235..9585)
                     /locus_tag="NBK22_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414921.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(9599..10900)
                     /locus_tag="NBK22_001738"
     CDS             complement(9599..10900)
                     /locus_tag="NBK22_001738"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCD2414922.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGQGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAINSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            complement(11179..>11312)
                     /locus_tag="NBK22_001739"
     CDS             complement(11179..>11312)
                     /locus_tag="NBK22_001739"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HCD2414923.1"
                     /translation="EVKASGGVRNLEDFNKMVEAGATRIGASAGVQIMQGLEADSDY"
BASE COUNT         3567 a         2214 c         1544 g         3987 t
ORIGIN      
        1 ccggtacttt caatgtttaa caatgaaagt accggtttta ttagcaaatt tatttatttt
       61 gttgatattt taatgaagct tcaataaatg atttaaaaat cgggtgcgga cgatttggtc
      121 tagataagaa ttctgggtgg aattgacaag caataaagaa atcatttgtc ggaatctcta
      181 ccatttctac taaacgtcca tctggacttg taccagaaat caccatacca tttgcttcta
      241 attgttctct atagtcatta ttaaattcat aacgatgacg atgtctttct tcaatttccg
      301 ctttaccata aacatcttgt gccaatgtgc cttctttaat tgaacatgga tataagccta
      361 agcgtaatgt accacctaaa tcttcgatat ctttttgttc tggtaataaa tctataattg
      421 ggtatggtgt tgctgggtct aattcagctg aatgtgcgcc ttcaaggcct aatacgtttc
      481 ttgaaaattc aactgttgca agttgcattc ctaaacaaat accaaagaat ggtacattgt
      541 tttctctagc atacttaatt gcactaattt taccttcact tgcacggaaa ccaaatccac
      601 ctggtactaa aataccgtcg acatctgcaa ggtattcggc tgcattttca tctgttactt
      661 cacttgaatc aatccatcta atgtcaatat ctttggcaaa aggatatcca gcatgtttca
      721 atgattcaac aactgataaa tatgcatctt gtaagctaac atatttacct actaaaccaa
      781 ttgtaatttt accatctaaa ttattaacga tatctaacaa ctgtttccat tcatcaagct
      841 gtgtttcata tttcgcgttt aattgtaaac gtttaataac gatatcatcc atattttgtt
      901 ggcttaattg taatggaatt tcgtataaag agtctgcatc acgacattca ataacacttt
      961 ctttattaat gtcacagaat aatgcaattt tatcttttaa atcttgtgtc atttcatatt
     1021 cagttcttac aacgattaag tctggttgaa tacctaagcc tcgtaattct ttaacactat
     1081 gttgtgttgg cttcgttttc atttctccag cagctttaat ataaggcagt aatgtacagt
     1141 gaacatacat aacattttct ctacctaaat cgctacgaat ttgacgaatc gcttcaataa
     1201 acggtaatga ctcaatatca ccagttgtac cgccaatttc agtgataaca acgtctgcat
     1261 tcgtactttc ccctgcaagt aataaacgtt ctttaatttc atttgtaata tgcggaataa
     1321 cttgaactgt tccgcctaag taatcaccac gacgttcttt tttcaatacg tgtgaataca
     1381 ctttaccggc tgtcacattt gaaaacttgt ttaaattaat atcaataaat ctttcgtaat
     1441 gtcctaagtc taggtcagtt tctgcaccat catccgttac gaatacttca ccatgttgat
     1501 aaggactcat tgtacctggg tcaacattta agtatggatc gaatttttga attgttacat
     1561 ttagacctct atcttttaat aatctaccta gagaagatgc tgtaataccc ttccctaatg
     1621 atgaaactac gccacctgtt acaaaaataa attttgtcat gtcttgacct cctaattatt
     1681 aaagggaatg cttcctcatc aattgtaaaa attacgtaaa acataacatt aaagcaagct
     1741 cattcaagtt gtttatttgt cgaattcgac aaaattaaat tctgtttact taccgtttcc
     1801 atactgctat gtcactatgt atatcattta caactttgat ttttgcgtta tcatttcttg
     1861 atttactcaa ttaaataaaa aaacgctccc tctcacaaat tctgtaaggg gagcgtataa
     1921 attttataca cgtgtcctat ttaaaggagc ccgaataaaa ttttatcatc tttcaactaa
     1981 aagtcaaaca aaaaattaat cgttgaagtc ttcttcgtct tcaaacactt cttcgtcttc
     2041 gtctagttca tcttcatctt cttcaatgac tatatccgaa tgattgattt cttcttcaac
     2101 ctgctcatct tctggatcat ttagttcttc ttgatcatct gtttgagctg gaatatcatc
     2161 gtcgtcatcc atttcatctt cgcccaataa ttttaagttt tgatcttctt catcatctgc
     2221 atccagaata tcgaattttt gaatagttgg tgcgattttc tcttcaatat catctaccga
     2281 ataccaatca cgtaatcccc ataaattttc tccaacattt aaaaaacgac catctgtgtt
     2341 taaatccgtg taaaattgta caacacgatt ttcaatttct tcgtactcat aatcacctaa
     2401 cgctctaaat tcatcgatga tatcatataa gttcattgtt tcgcctttat cattcaataa
     2461 tgtataagcc atatcaataa atgatttttc atcaaccatt tgttttgtat aatcttgaat
     2521 tttcattgcc caatacttcc tttcgaagga tataatctaa atttatacgt ctgaattcat
     2581 ttatatatag taacaaaact aaattataaa tgacaatatc atatttcgca atcactgtta
     2641 gtaatttaaa tatttttcaa aaacgacata atcatcagtt tgttcgattt caacgaaatt
     2701 actatttaga cataatggtt taaaatcatt attctctcca aacagaatca tcgtaatcac
     2761 attaaggttg tcatagtttt ttcttaagta agttggcaat tgtcgcagcg cacttgaacc
     2821 aataccatta aagcgatatg atttatccac ttcaatagca gcaatgttca tgcgttcacc
     2881 aatctgctcc aatgcgataa agccaacctt ttggccatca atcattaaat taatgatatg
     2941 tacattggtc acttctgaac gactttcttc ttcaagcttt aatgacgaaa catgttttga
     3001 atctaagtcc aagtaatata gtcgttcttt gccaattggt ccttcttctt tagtcgctgt
     3061 atgcatgaat ccagcacgtt cgtaaaggtt ccaagcattg tcattttccg catctactac
     3121 tagatataga tgattaaaat caggaaatac accttgaaca tattgcggca atgacatcat
     3181 tattttcgtg ccatatccaa aaccttgata tttttcatta atcgataatg aacgaatata
     3241 aacgacattt tcaggtgtat cataaccttc atgctgataa taacgatgta atacaaaaaa
     3301 gcccaccact tcatttttat cgtttattgc tacgttagca atcctatcag catcattaat
     3361 tgcatcatca ataacttctt taggtaaaga tgaatatatt tgttgtcttt cattcaagtc
     3421 aaaatcatta attgctgaac gatattgttc atcaaatgct tttaaagtga taccttcaaa
     3481 acaaagttct ttagccatat atttaacccc tatcttttta tatatgaagc atgaatcccc
     3541 atattatttt acctattatt tcactacgat accattattt gctctaagtt aaataaatta
     3601 cttatttata ttttttgaat tttaaaatta atgtgctact cctcattact tatcatttta
     3661 atcagagact tacaaataca ctgcgtacga actactttta atattgcgtc actaactaac
     3721 aatcctatac aacttcaaaa ttcctagaaa ataatatata taacttaacg tctacgtttg
     3781 taatacatta attgcgatac gcttacaatg ttgaaataca atggcatcaa atttgttaat
     3841 aattactatt attacttgtt ttttagtcat tttactatac tattatttaa ggatagacat
     3901 taataatgag gtgggaatct tatgaaagtt ggcattgacg ctggcggtac actaattaaa
     3961 atcgttcaag agcaagataa ccaacgtact tttaaaactg aattaactaa aaatattgat
     4021 caagttgtgg aatggttaaa ccaacagcaa attgaaaaat tatgcttaac tggaggtaat
     4081 gcaagtgtca ttgctgaaaa catcaacatt cctgcacaaa tttttgtaga gtttgatgct
     4141 gcatctcaag gactaggtat tttgttaaaa gagcaaggcc atgacttagc agattatatt
     4201 tttgcaaatg ttggtaccgg tacgtcacta cattatttcg acggccaatc acaacgtcgt
     4261 gtaggcggta tcggtacagg tggtggcatg attcaaggac tcggttattt actaagtcaa
     4321 atcactgatt ataaacagtt aactgacatg gcacaacacg gtgatcgtaa cactatcgat
     4381 ttaaaggtta gacatattta taaagatacc gaaccaccca ttcccggtga tttaacagca
     4441 gctaattttg gtcatgtact acatcattta gatgctgatt ttacacctag caataaatta
     4501 gcagccgtta ttggtgtcgt tggtgaagtt gttacgacaa tggccattac tgttgcacgt
     4561 gaatttaaaa ctgaaaatat cgtttatatc ggttcttcat ttcataataa cgctttatta
     4621 cgcaaagttg tggaagatta tactgtgtta cgcggttgca aaccgtacta tgttgaaaac
     4681 ggcgcatttt caggtgctat tggtgcatta tatttaggta aatagaaaaa gaccatttat
     4741 atgcgcgttg ttttaaataa acgctatata aatggccttt tattttatgg tctaagtgtt
     4801 ttgaatacgt attgttccat ttattatcac ttaattaatt acaactcaat tcaattaaaa
     4861 tgtaagttca ttagacaatt tttgcgctga aacaattgta ccaaccacgc cagtttttaa
     4921 atctatcgca acggctttat gatcatctct aagttcatct agccattctc cgaaaacaga
     4981 accttcaatc gtaattaaat cacactcttc aaaatcttca tctcgaactt ttaacgctct
     5041 gtttttaata ctccatacag gcataatatg cggctttttt tcgtcgctat cattttcact
     5101 aatgagaata aactgcttac tacctttttt agttaagccg tatacttcat catattttgc
     5161 tacatcttgg acaaattctt tgattctaat tttatctaat tccgtcatca cttcttgtgt
     5221 aacagctaca acgtttagta gatgcccttc ttcttccatt gtttgattca taataatatg
     5281 gtcttgttca tcaaacaatt catctaattc ataagtagca aaacgatcaa cgtccatttt
     5341 tacaacttta tcaaatggaa tatcacgact tgttaaaaat gatacggcca cactttcttc
     5401 tgtccaaacg cctacatacg cttcactttt ataatttatg cgtactagtt ttttattttt
     5461 agaggcgaaa taaatacatt catttattaa tatatctcta aagaatggat atgctttata
     5521 agacatgctt gaccctcact ttttaaaata tgtcatacat aaaatagtaa atgactccaa
     5581 caagttagtc aattagacat attatgaatt caatttaatg tcatgttttt cattttaaaa
     5641 attcaatagc attaaatttt acgtatacac aaattcggtc tataacacga aacatatcta
     5701 gttactaaaa atttcaaaac gataaccttt agtatacctt tatcaacaaa atggcaactc
     5761 ttataacata gtgaaataaa agtcgccatt taatattagt attacaaagt attgccatat
     5821 cataaattta attactgttg actagaatcg aaaaaagctt gttacaaacg catattcagt
     5881 caactactgg caacacaaca ttgtagcgcc ttatgttccg aactctaata gtgttcaagt
     5941 tcagctaaaa atgcgcgttc attctccagt actgtttctt ttgaatcacc atgccaaata
     6001 cagccaaacc ttgatttaac tttatcttca ccgtaccaat caccatcata ataagataac
     6061 gggtataaac taccttcttt gacatatttc aaaatcgtat tgaagaaatg tgtgttatca
     6121 ccttttgaat gataactata aaacttttga tatcctgaat tcaactcgtt agcaagtaat
     6181 aacatgctag ttgaaccatt ttgtctaaag tttaaatcaa tcgcataaac gttatcatcc
     6241 tcatcgacga gtaggtcaaa accagctaca ccaaagaatc cttgattcac accgttttcc
     6301 ataatttgtc tacctgcttc tataacgtgc tctggtacat tagtagtatt ttcatttccg
     6361 ttataaaatc cgtacttatc cgttaattgc gttcccgcac caagatattg aatgcctagc
     6421 gattccgagt atgcaaactg aacgcaataa ttcgcttttt cttcaatctt ctgttcgata
     6481 ataagcgaat tcgtttcagc agttgcctgt ttaatacgcg ttatcgcttt ttgtaaatct
     6541 gcatcatgat aacaaatcat aacgccatag ccacctgctg tcggaagatc atctcccggc
     6601 ttaattacaa acgggaattc ccaattctta atctcgtttt cgaattgctc aatttttaca
     6661 acttttcttt ttggtaaaaa cttcccattt gtccattcag gtattcttgc tttattattt
     6721 aaagcaacaa ataacgtttt atctaatgca taatattgct gattcaagat tgtttcatca
     6781 tgaatatatt gaaaataaat ctttttattt tccttatgtg ccaattgttt gatcaagttt
     6841 tcgtaagatt gctgattgtt aaatgtataa attgagttcg gtacttcctt accaataact
     6901 tgaaatagct gatgcaattt gtctgtcgca ctagcttcgt gaacaataac aggtaattga
     6961 tttgctatta ataactccct accagttaaa aaattagatt gatgttcgtc cggtttcaac
     7021 catggattcg atatatacga aggtcttgac gtatagacaa catctttgtc atataaatca
     7081 cttaacgtta agttcggctc attaccatta tttgtcatta cttcccattc cctttcaaat
     7141 gcgcatgctc ttcaataatg tcttgataaa cgtcttgatt tgtaattaac tctaacccca
     7201 tcaacgccat tattttagca cctttaatta atgcttcatc accatgtaca ctcgcagccg
     7261 cttctctaaa tctatgcgta tgtcctacta aattacgtga tcctatttta atatgaggat
     7321 gtattgttgg cacaacatga cttacgttcc ctgtatccgt agagccataa ccaaaatcat
     7381 catcaataac tgcttcacca acttcttcag catatttagc aaataaatca tctaatttcg
     7441 gcgttttaat gaattcattc acaccgtttt gaattggacc aaattcataa tcacaaccag
     7501 tctgtatcgc agctccacgt gcgatttgat ttactttttc tgttaatata tccaattctt
     7561 tacgcgtcat tgctctagta taaaaacgag catgtgtata gtctggaata atattagctg
     7621 ctttcccgcc atctaaaatc acaccatgca cacgttgatc ttttttaata tgttgtcgta
     7681 gttgtgctac accattaaaa taactaatca tagcgtctaa tgcatttaac gcttcatctg
     7741 cattttcaga ggcatgagca ctttttccgt aaaatttaac atctaaaaca tccactgcca
     7801 aagtatcaat cgttttataa gtttcatttc ccggatgaat cattaaggca atgtctattt
     7861 gatcaatcac accagccttg acataagaag ctttagcgct accattttcc ccaccttctt
     7921 cagctggaca tccaagaacg actactttac caccaatttg gtcaatcact tgcttcaaac
     7981 caattgcacc aagcacactt gcagttccaa tgatattatg accacaagca tgacccaatc
     8041 ctggcaaagc atcgtattct gctaaaaaac ctatagctgg cccgtcaaga cctgaatcat
     8101 atgtcgctat aaacccagtt gcatgcccag caatctcggt ttcaatttca aaatcatgct
     8161 ctttcaaacg atctattaaa gttcgagacg caaatatttc ttcattacca agttcaggac
     8221 gttcatgaat tctatgactg atttcgatat aactatattt atttgtttct atataatcga
     8281 gaatttgttg tttttcactc attttttact atctcccttt taccctacac tcattttaat
     8341 catccacaat attttgttct ttcaaaatga attattactt attctatcgg ttttatctca
     8401 tgatgtcatc tagtttttct ttatttacaa aattttctaa taactaaaag cctttcataa
     8461 atttataaaa cagtttcaaa ttgtaaaaca taagccctat tgttacaatt taagtattgt
     8521 tagaaacaca acttacaaca aacttaagtt ttataatgca acaaatcata agcgttttat
     8581 agttttgagt agttaatagg aggaaattca aaatgacaaa aatgaatgtt gaaagtttca
     8641 atttagatca tactaaagtg gttgccccat ttattagatt agcgggaacg atggaaggat
     8701 taaacggaga tgtcattcac aaatacgaca ttcgtttcaa acaaccaaac aaagaacata
     8761 tggatatgcc cggactacat tcattagaac atttaatggc tgaaaatatt agaaatcata
     8821 gtgacaaagt tgttgattta agtcctatgg gttgccaaac tggtttctat gtatcattta
     8881 ttaatcatga taattatgat gatgtattaa atattgttga agcaacttta aatgatgtgc
     8941 taaatgctac tgaagtgcct gcttgtaatg aagtacaatg tggctgggca gcaagtcatt
     9001 cattagaagg tgctaaaact atcgctcaag catttctaga caaacgaaac gaatggcatg
     9061 atgttttcgg tacaggaaaa taaatcttag tcaatcaagt taatcagaaa agcagtcgaa
     9121 caatgatgtt acaatcgcca ttgtccaact gctttttatt atgcttcaaa gtcaaaaaat
     9181 cgaacaaatg aaaaagtaaa atctttaaca tttgtccgat ttatttgaga accactataa
     9241 tttcttaatt agtcccatta acacgaactg cataggtaac cttaaatata gttgccatgt
     9301 tggcaattgt ttatctccta aaggtaactt tttaactgcc atatagatat tagctgggaa
     9361 tacagctagc aagaatagat tgattgtatt tttcaagcat tgagatggtc ttttaattaa
     9421 aagtgcaagt ccaaataata tctcaaagat tcctgtaaca agaaccgctg tttttcgaag
     9481 tggcaaacat ttcggtatga tatttctaaa ttgtcgttct cgtgtaaaat gcaatacacc
     9541 tattacacta aaacctattc ctaataaata tcttagtatg ttcaatcagc ttcaactcct
     9601 attctgtaat gattttatga attaatgtag gcgatacaac atgatcagca attgttatgc
     9661 ttgaatctaa ttttttaaca acatcgtcta catcttggcg attactgtga atcgttaata
     9721 atgattctcc ttcttctact ttatcaccaa tttttttatt taaaacaata ccaaccgcta
     9781 aatcaatatc atcctctttt gttaaacgtc ccgctcctaa catcatcgaa gcgacaccta
     9841 tatcgttaga gactaattca gtcacataac ctgatttttt agctttatat tcaatttgat
     9901 attgagcttg tggcaaacgc tctggatggt caataacagt ttcgtcgcca ccttggtttt
     9961 taataaatgt tttgaatttt tctaatgctg cacctgaatt aattgcctca attagcaacg
    10021 ctctcgcttc ttcaagcgtt tcagctttgt ttgcaagtac aaccatttga gaacctaatg
    10081 ttaatacaag ttctgttaaa tctttcggac cttgtccttt caacgtatca attgcttctt
    10141 gtaactcaag cgcattgcca atcgcacgtc caagtggctg attcatatca gaaataatcg
    10201 ccatcgtatt acgtcccaca ttattaccaa tacgtaccat tgcgtgcgct aatgcttcag
    10261 catcttctaa tgttttcata aatgcaccgc taccagtttt tacatctaat acaattgcat
    10321 ctgcaccagc agcaatcttt ttactcataa ttgaagaggc aattaatggt attgaattga
    10381 cagtaccagt aacatccctt aaggcatata attttttgtc tgcaggagtt aaatttcctg
    10441 attgtcctac aactgccact ttattttcat taaccaattt cacaaatgtt gcttcatcta
    10501 tttcaacatg aaaaccatca attgcttcta atttatcaat cgtaccacct gtatgaccta
    10561 atccacgccc actcattttt gcaacaggaa catctacagc tgctactaat ggtgctaaaa
    10621 ccaatgtagt tgtatctcct acaccacctg ttgagtgctt atctactttg acacctttaa
    10681 tatcactcaa atctatcata tcaccagaat taaccatagc catcgttaat gctgcacgct
    10741 catcatcatt catatcttgg aaataaatcg ccattgctaa acttgatgct tggtaatcag
    10801 gaatatcccc tttaacatag ccgtcaataa agaaattaat ttcttccgtt gttagtgtat
    10861 gaccgtcacg ctttttctca ataatgtcta tcattctcat ttttatcatc cttttcttaa
    10921 aaagcttagg acaaagcatc tgcgctttct ctagtccatt tttaaaagca caagcgaaaa
    10981 ttattatagc aagctatcga tttcattttt aatatcacaa tttcaatgcg atgttattat
    11041 tcttaaatag attggttata acgttaaagt ccctattaaa ttatcttaga atcatcatgg
    11101 catttatgat gtcttaaagc tgatatcgac atacttatat atggttacga tgtcccatgc
    11161 ttacatattt ttataaaatt agtaatctga atctgcttct aaaccttgca taatttgaac
    11221 gcctgcgctc gcaccaatac gtgtcgcacc tgcttcaacc attttattga aatcttctaa
    11281 attacgtacg ccacctgatg cttttacttc ta
//
LOCUS       DAHHYJ010000086        11307 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000086 DAHHYJ010000000
VERSION     DAHHYJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.086"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(415..1167)
                     /locus_tag="NBK22_001740"
     CDS             complement(415..1167)
                     /locus_tag="NBK22_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCD2414924.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKDERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHLINTNEFDQFFAIS"
     gene            1335..2321
                     /gene="pta"
                     /locus_tag="NBK22_001741"
     CDS             1335..2321
                     /gene="pta"
                     /locus_tag="NBK22_001741"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCD2414925.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLDLDISNIELINPATSELKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDV
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2324..3160
                     /locus_tag="NBK22_001742"
     CDS             2324..3160
                     /locus_tag="NBK22_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD2414926.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFENENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            3747..4667
                     /gene="mvk"
                     /locus_tag="NBK22_001743"
     CDS             3747..4667
                     /gene="mvk"
                     /locus_tag="NBK22_001743"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCD2414927.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            4672..5655
                     /gene="mvaD"
                     /locus_tag="NBK22_001744"
     CDS             4672..5655
                     /gene="mvaD"
                     /locus_tag="NBK22_001744"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCD2414928.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            5668..6744
                     /locus_tag="NBK22_001745"
     CDS             5668..6744
                     /locus_tag="NBK22_001745"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCD2414929.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            6921..7262
                     /locus_tag="NBK22_001746"
     CDS             6921..7262
                     /locus_tag="NBK22_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCD2414930.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(7314..8636)
                     /locus_tag="NBK22_001747"
     CDS             complement(7314..8636)
                     /locus_tag="NBK22_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCD2414931.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNVLDQHDDIVDSITAPHIIINTGATSVIPNIKGLDQAKHVFDSTGLLNISYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     YTNVETTELSSDNHHTTVHTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTGKEAAAQHYDYTEYQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(8623..9063)
                     /locus_tag="NBK22_001748"
     CDS             complement(8623..9063)
                     /locus_tag="NBK22_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD2414932.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMIDTIRGKDGGYLANDQSADVSLATLYKHFVLEKEHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            9121..9222
                     /locus_tag="NBK22_001749"
     CDS             9121..9222
                     /locus_tag="NBK22_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414933.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVDALTVRLHN"
     gene            9688..11085
                     /locus_tag="NBK22_001750"
     CDS             9688..11085
                     /locus_tag="NBK22_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2414934.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLNNNLKKHASDNQQAISSNQQIIRTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSILKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSGAGAVVTVGASILLGSNPVGWA
                     VIGGFVAGTLVSKAADWAYQSNWNGIKDKTDWVGHKIDDSIDVVKKSTEKAVDSVGEA
                     TKSISNHLNPMKWSW"
     gene            11086..>11307
                     /locus_tag="NBK22_001751"
     CDS             11086..>11307
                     /locus_tag="NBK22_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD2414935.1"
                     /translation="MLLYESNIINKNPRYRIIKYKNDYLMIDLVSTWLVLFFPFINWL
                     IPKKYVQISREEFDNLNIVKPVKNKALWPV"
BASE COUNT         4011 a         1617 c         2161 g         3518 t
ORIGIN      
        1 gaatttttag tagttaaaat tctcttgaag tataaaaagg aaaaattatt gaaaaaaatt
       61 cgacaaaaat atgtttcaaa tatttcaaat aggttaaaat aatattagtt aaccattaca
      121 aaaattgtat agagtagcga ctgtataatt tctattgagg ttaacgttta tatgtagtga
      181 tagtagttaa agttctccca aggaagacta ctcgggtaca ctttgctatg agcaaagtgt
      241 actttgttat tgataataca ttagcacata tatataagtt taaacataca gatttcaact
      301 atttaccaaa tcacgtctta tgtatacggg aatatactga agataaacga aaaattccaa
      361 gcttaaaccg ataagcttgg aattttttta ttaatttata aacgtaccaa tgtattaaga
      421 aatcgcaaag aattgatcga attcgtttgt gttaataaga tgtcctacaa agaaactacc
      481 gaattcaccg tatcgtgctg ttgtttcatc aaagcgcatt tcgtatacaa tttttttgaa
      541 ttgtaatacg tcatctgaga acaatgttac gccccattcg aaatcatcaa accctacaga
      601 accagtaata aattgtttga ttttgccagc atattttcta ccaatcatac catggtcata
      661 cattaatttt tggcgttctt ccatagttaa catgtaccag ttataagttt cattacgacg
      721 tttgttcatt ggatagaaac aaatataatc agaatgtggt aattctgggt ataatcttgc
      781 tttgatatga gggttctcat aaggatcttc atcagattta ccagctaaat aattgctcaa
      841 ttcaatgact gatacatatg aatatgtagg gattaagaag tcagcaatgc gcaatttgtt
      901 aaattcattt tcaatatgat ttaaagactt catttcagga cgtaagaacc ataataacaa
      961 atctgctttt tgaccagtta tattataaat agcttgatca ccagattttg atgatcttac
     1021 agttgctgta ttttctaaaa atgattgaaa ttcagtgaca agtgcatcgc gttcgtcctt
     1081 tggaactaga cgtaatgatg cccaatcaac tgcataaaat aaatgtagac tataccaacc
     1141 atctaatgtt tcggctgctt gactcatgtt tatcgctcct tttcaaaaat catcagttct
     1201 ttataaattt tatcataaag caaaggggta acgataaaat tatgattaca aattggtgac
     1261 gtggcattat gaaataaaat ggcgtataat tataccgtga atgattaata agatttatat
     1321 tacaggagga cattatggct gatttattaa atgtattaaa agacaaactt tctggtaaaa
     1381 acgttaaaat cgtattacct gaaggagagg acgaacgtgt tctaacagct gcaacacaat
     1441 tacaagcaac agattatgtt acaccaatcg tgttaggtga tgagactaag gttcaatctt
     1501 tagcgcaaaa acttgatctt gatatttcta atattgaatt aattaatcct gcgacaagtg
     1561 aattgaaagc tgaattagtt caatcatttg ttgaacgacg taaaggtaaa gcgactgaag
     1621 aacaagcaca agaattatta aacaatgtga actacttcgg tacaatgctt gtttatgctg
     1681 gtaaagcaga tggtttagtt agtggtgcag cacattcaac aggagacact gtgcgtccag
     1741 ctttacaaat catcaaaacg aaaccaggtg tatcaagaac atcaggtatc ttctttatga
     1801 ttaaaggtga tgtacaatac atctttggtg attgtgcaat caatccagaa cttgattcac
     1861 aaggacttgc agaaattgca gtagaaagtg caaaatcagc attaagcttt ggcatggatc
     1921 caaaagttgc aatgttaagc ttttcaacaa aagggtctgc taaatcagac gacgtgacaa
     1981 aagttcaaga agctgtcaaa ttagcacaac aaaaagctga agaagaaaaa ttagaagcaa
     2041 tcattgatgg cgaattccaa tttgatgctg cgattgtacc aggtgttgct gagaaaaaag
     2101 cgccaggtgc taaattacaa ggtgatgcaa atgtctttgt attcccaagt ttagaagctg
     2161 gtaatattgg ttacaaaatt gcacaacgtt taggtggata tgatgcagtt ggtccagtat
     2221 tacaaggttt aaattctcca gtaaatgact tgtcacgtgg ctgctcaatt gaagatgtat
     2281 acaatctttc aattatcaca gcagcgcaag ccttacaata acgatggatt tagcgagtaa
     2341 atattttaat ggcgtcaact ggcgatatat cgatcattct tctggattag aacctatgca
     2401 atctttcgca ttcgatgata cattttgcga aagtgtgggc aaagatatat cagataatgt
     2461 tgtgcgtact tggattcatc aacatactgt tattcttggt attcatgatt caagattgcc
     2521 gtttttaaaa gatggcattg attatttaac gaatgagatt ggttataatg ccattgttag
     2581 aaattctggt ggcttaggtg tcgttctaga tcaaggtgta ttaaatatat cgctgatgtt
     2641 caaaggacaa acagaaacaa cgattgatga agcgtttact gtgatgtacc tcttaattag
     2701 caaaatgttc gaaaatgaga atgttgatat tgatacgatg gaaattgaac attcttattg
     2761 cccaggaaaa tttgacttaa gtatcgatgg taagaaattt gcaggcatat cgcaacgaag
     2821 agttagaggc ggtattgctg tacaaattta tctttgtgtt gaaggctctg gttcagaacg
     2881 tgcattgatg atgcaaacat tttatgaaca tgctttaaaa ggtgaagtga ctaaatttaa
     2941 atatcctgaa attgaaccat cttgtatggc ctcattagag acattgctta acaaaacgat
     3001 tactgttcaa gatgtaatgt ttttactatt atatgcaatc aaagatcttg gcggtgtatt
     3061 aaatatgacg ccaattactc aagaagaatg gcaaagatac gatacgtatt ttgataaaat
     3121 gattgaaaga aacaagaaaa tgatagatca aatgcaatag ttaaatattt aacagcccct
     3181 ttcaagttat cgtttggggc tgttattttt gaaagtatcc ataaaaagca taaatatgtt
     3241 attgacctaa tatttaaatt gaacatcatt aaattaaaag taatgatatg atggaaataa
     3301 taaatttaat aagaaaattt catgttttac gtattaaata gattgagaat agggtatata
     3361 taatgcgtat gactatgaaa gaaacgacct tctattgaaa tgttctaata ctttagtagt
     3421 aaatctatat attccaatta ttatttgtag tattatagtt agggtggaaa attataaatt
     3481 gtagggctat tttataaaaa gataaagaat ctgaaatatc actcgataag atttaaactt
     3541 tgaagtaaac gaaagttagt tttatactaa tcaagttaag tcaaatataa attcaaaata
     3601 aagattatac ataatatgaa taatgtgaga taaagactgc aatgaaattt aacgcgaatg
     3661 cgtatgcaac ttagaatatg atgtctttta ctaataaata aaaataatga atcaagtata
     3721 ggataaatat aaaaggtgat attgacatga caagaaaagg atatggggaa tcgacaggta
     3781 agattatttt aataggagaa catgctgtta catttggaga gcctgctatt gcagtaccgt
     3841 ttaacgcagg taaaatcaaa gttttaatag aagccttaga gagcgggaac tattcgtcta
     3901 ttaaaagcga tgtttacgat ggtatgttat atgatgcgcc tgaccatctt aagtctttgg
     3961 tgaaccgttt tgtagaatta aataatatta cagagccgct agcagtaacg atccaaacga
     4021 atttaccacc atcacgtgga ttaggatcga gtgcagctgt cgcggttgct tttgttcgtg
     4081 caagttatga ttttttaggg aaatcattaa cgaaagaaga actcattgaa aaggctaatt
     4141 gggcagagca aattgcacat ggtaaaccaa gtggtattga tacgcaaacg attgtatcag
     4201 gcaaaccagt ttggttccaa aaaggtcatg ctgaaacgtt gaaaacgtta agtttagacg
     4261 gctatatggt tgttatagat actggtgtga aaggttcaac aagacaagca gtagaagatg
     4321 ttcataaact ttgtgaggac cctcagtaca tgtcacatgt aaaacatatc ggtaagttag
     4381 ttttacgtgc gagtgatgtg attgaacatc ataactttga agccttagcg gatattttta
     4441 atgaatgtca tgcggattta aaggcgttga cagttagtca tgataaaata gaacaattaa
     4501 tgaaaattgg taaagaaaat ggtgcgattg ctggaaaact tactggcgct ggtcgtggtg
     4561 gaagtatgtt attgcttgcc aaagatttac caacagcgaa aaatattgta aaagctgtag
     4621 aaaaagctgg tgcagcacat acttggattg agaatttagg aggttaatgc gttgattaaa
     4681 agtggcaaag cacgtgcaca tacgaatatt gcacttataa aatattgggg taaaaaagat
     4741 gaagcactaa tcattccaat gaataatagc atatctgtta cattagaaaa attttacact
     4801 gaaacgaaag tcacttttaa cgaccagtta acacaggatc aattttggtt gaatggtgaa
     4861 aaggttagtg gcaaagaatt agagaaaatt tcaaaatata tggatattgt cagaaataga
     4921 gctggcatcg attggtatgc agaaattgaa agcgacaatt ttgtaccaac agcagcaggg
     4981 ttggcttcat cggcaagcgc atatgcagct ttagcagcag cttgtaatca agcgctagac
     5041 atgcagctgt cagataagga tttatcgaga ttggcgcgaa ttggttcggg ttctgcgtcg
     5101 cgtagtattt atggtggatt tgcagaatgg gaaaaagggt atagtgatga gacgtcatat
     5161 gccgttccac ttgaatcgaa tcattttgaa gatgaccttg ccatgatatt tgttgtgatt
     5221 aatcaacatt ctaaaaaggt acctagtcga tatggtatgt cattgacacg aaacacatca
     5281 aggttttatc aatattggtt agatcatatt gatgaagatt tagctgaagc aaaagcagcg
     5341 attcaagaca aagattttaa acgccttggt gaagtaattg aagaaaatgg tttgcgtatg
     5401 catgccacga atctaggatc aacaccgccg ttcacatatc ttgtgcaaga aagttatgat
     5461 gtcatggcgc ttgttcacga atgccgagaa gcggggtatc cgtgttattt tacaatggat
     5521 gcgggaccta atgtgaaaat acttgtagaa aagaaaaaca agcaacagat tatagataaa
     5581 ttattaacac agtttgataa taaccaaatt attgatagtg acattattgc cacaggaatt
     5641 gaaataattg agtaaggaag agataaaatg attcaggtca aagcacccgg aaaactttat
     5701 attgctggag aatatgctgt aacagaacca ggatataaat ctgtacttat tgcgttagat
     5761 cgttttgtaa ctgctactat tgaagaagca gaccaatata aaggtaccat tcattcaaaa
     5821 gcattacatc ataacccagt tacatttagt agagatgaag atagtattgt catttcagat
     5881 ccacatgcag caaaacaatt aaattatgtg gtcacagcta ttgaaatatt tgaacaatac
     5941 gcgaaaagtt gcgatatagc gatgaagcat tttcatctga ctattgatag taatttagat
     6001 gattcaaatg gtcataaata tggattaggt tcaagtgcag cagtacttgt gtcagttata
     6061 aaagtattaa atgaatttta tgatatgaag ttatctaatt tatacattta taaactagca
     6121 gtgattgcaa atatgaagtt acaaagttta agttcatgcg gagatattgc tgtgagtgta
     6181 tatagtggat ggctagcgta tagtactttt gatcatgaat gggttaagca tcaaattgaa
     6241 gatactacgg ttgaagaagt tttaatcaaa aactggcctg gattgcacat cgaaccatta
     6301 caagcacctg aaaatatgga agtacttatc ggttggactg gctcaccggc gtcatcacca
     6361 cactttgtta gcgaagtgaa acgtttgaaa tcagatcctt cattttacgg tgacttctta
     6421 gaagattcac atcgttgtgt tgaaaaactt attcatgctt ttaaaacaaa taacattaaa
     6481 ggtgtgcaaa agatggtgcg tcagaatcgt acaattattc aacgtatgga taaagaagct
     6541 acagttgata tagaaactga aaagctaaaa tatttgtgtg atattgctga aaagtatcac
     6601 ggcgcatcta aaacatcagg cgctggtggt ggagactgtg gtattacaat tatcaataaa
     6661 gatgtagata aagaaaaaat ttatgatgaa tggacaaaac atggtattaa accattaaaa
     6721 tttaatattt atcatgggca ataaatgatt tgatagggga gcaatcatct aatttaatta
     6781 gaagataaat gctccatttt ttattgtcag ccgtgttacg tatcttgaaa aaaatattaa
     6841 aaagagtaaa atagatagag ttaacgaaaa aattaatgaa atcgacgata tagatatgat
     6901 aaagaaaggt gggtagcaat atgaaaaata cattccttat ttgtgatgaa tgtcaggcag
     6961 tcaatataag aacgttacaa aagaagttgg aaaaattaga tcccgatgct gaaatcgtga
     7021 taggttgtca atcttattgt ggacctggac gccgaaaaac attcactttt gttaataacc
     7081 gcccactggc tgcgcttact gaagaagaat taatcgaaaa agtttctcaa caattaaaga
     7141 aaccacgtga tcctgaagaa gaagagcgtt taagaaaacg acatgaagaa cgtaaacgtc
     7201 gtaaagaaga acaagataga aagcttaaag aaaaattaga aaagcgaaaa gcacaacaat
     7261 aaaacctgat ggcagcatca ttcaatgcgt gccaccaggt ttttatgttt tgtctagaaa
     7321 ttaaataaat cattaaatga ttcggccatc gtaggatgcg tataaatatt atctcgtaat
     7381 acggtatatg gaatgttttg atcaatcgca agtttaatta tattaattaa ttcttcagat
     7441 tgcttaccat ataatgtagc acctaaaatc atattatttt cattattaat gactacttta
     7501 aataaacctc ttggatcatt gttaattttg tgacgaggta tagcacttac taaaagttga
     7561 tattcagtgt aatcataatg ttgagcggca gcttctttac cagttaatcc aacacgtgat
     7621 aatggtggat ctataaatac tgtataaggc acgctgcctc tattgtcagt cgtacgtgac
     7681 tgattaccat ataacgctga tttgataatt cgataatcat ctaaagatat atacgtaaat
     7741 tgaagtccgc ctttaacatc acctgcagca taaatatgcg gcacagttgt ttgaagatga
     7801 gcattgactt taatttcgcc tctgtcgcct aattcgatat cagtattttc taaagctaaa
     7861 tccgtattcg gtttgcgacc gatagccaaa agtactgcat cagcctcaaa gttaccaacg
     7921 ttagtatgga ctgttgtatg atgattgtca gatgacaatt cagtcgtttc aacatttgta
     7981 tacaatgcaa tgcctttatt ttctaagtca gtaatagcat gtgcaacgac atcttgatct
     8041 tcacgtggca taaatgattc accacgttct aatactgtaa ccttactacc taaattcgca
     8101 aacattgaag caaattctaa ggcgatataa ccgccaccta caataacgag gtgcttaggt
     8161 tgatagctaa tgtttaataa acctgtcgaa tcgaagacgt gtttagcttg atcaaggcct
     8221 ttaatgttag gaatgacaga ggtagcaccg gtattaataa tgatatgagg tgcagtaata
     8281 ctatcgacga tatcgtcatg ttgatctaat acattcactt cagtattaga tttaaactgc
     8341 gctttaaaat ccagtacatc aatgttgtta tcgtctgcta ataagtggta atttttattg
     8401 tttagcgcat tgacaacatc gtttttacgg ttataacttg cttcaaaaga tttgccttct
     8461 aatccatcat gtacaagtgt cttcgaaggt atacatccta tatttataca agtgcctcca
     8521 tacattttcg aagattgttc gataactgcg acgtgttgac ctgttgatgc agcgtatttc
     8581 gctaaagttt taccagcttt cccaaatcct attacaatta aatcatatgt tttcatgaca
     8641 taaatcctcc ttttgaatgt cttcaatgac atctttgatt gtttttccat tataaaaatt
     8701 aataatgata ttctgttcgt cttgctgata atgtgacatg gtagttgcaa tattacgagc
     8761 aatttgacag tgactgcctt cgtcgccagt aaatagacgt gtgtggtgtt ctttctctaa
     8821 gacaaaatgt ttatataatg ttgctagaga gacatcagca ctttgatcat ttgctaaata
     8881 accgccatct ttacctcgta ttgtgtcaat catttttaaa tcgacaagtt gagtcgtcac
     8941 gcgtcgtaat tgaacaggat ttaaacaagt taattctgct aatgaactac tattgaattt
     9001 ttctgaatga tgcttagtta aaaaagctaa tacatgcacg gcaatgttaa attctaaatt
     9061 caatgtttct cacctcataa ttgtaactgt attagttgca attaaaaatc attttgctca
     9121 atgtgtcaat acttatgttt gaaaagtgtt gaattaggtg tggttattgt gatgatttat
     9181 tgtttgaaag ttgatgcatt aacggtgaga ttgcacaatt gaaattttaa ttatgtatga
     9241 actgatagat atgaaatgaa tattgaaaat gagagcaata aaattttgaa tgatgatgtt
     9301 gtgtgattgt ataaacttat tcgtatttta gatacgtttt gatatttcgc atgatgagga
     9361 aatactttta acaactgatg acatatttta atagtgaata tattttgaga tattaatata
     9421 tttaatgtta aggatgttgt cgcaataaaa ttaaaatatt gaaagataat ttttattgat
     9481 taattgcgaa tatacaaaga caccattata tccatatgct aaaataagaa aaaaggcgac
     9541 atgttatctc aatttacctg catctagact cctttacata agaaataatc atcgccgagt
     9601 tttaaaattt tacaactttg tagatgtaaa aagcatttga ttttaaatag tttgaaaatc
     9661 atatattttt gaaaaaggag catgccgatg agtattgaca tgtatttaga cagatctcga
     9721 aaccaagctt caagtgtggg gaatttgagt caaacaatga attcaaatta tgatgcgttg
     9781 gaaaaagcaa ttactcaatt tattaatgat gatgcgctta aagggaaagc gtatacgtca
     9841 gctaagcaat tttttagtac ggtgttaatt ccattatcaa caagtatgaa aacattgagt
     9901 gatttaacga agcaagcttg cgataatttt gtgtcacgtt atacgagtga ggttgatagc
     9961 atatctttaa aagaatcaga gcttgaagaa gatatcagat cattaagtca acaaattacg
    10021 cgatatgaaa atttgaataa caatttgaaa aagcatgctt ccgataatca gcaagccatt
    10081 tcatcgaacc aacaaataat acgaacatta ggtcaacaaa aacatgaatt agaagagaag
    10141 ctacgcaaat tgcgtgagtt taatcaaaaa tcaccagaaa tatttaaaga agttgaagaa
    10201 tttcaaaaaa ttgtccaaca aggacttacc caagcgcaga atttttggaa cttttcaaca
    10261 aatcaattta atataccttc aggtaaagaa cttgattggg ctaaagcaag tcatgaaaaa
    10321 tatttgaaag ttgctatggg gaaaattgag cataaagcag agaaagaaac tttaaataaa
    10381 gcagactttg ctgttataaa ggcatatgcc aaagagcatc cagaagacga tatcccgaaa
    10441 agtatactga aatatataaa tgacaataaa gacagtatta aaagagatat aggattagat
    10501 atcacttcaa cacttttaga gcaaggcggt ataaatgcaa gtaaattcgg tgtatttatc
    10561 aatacagcag gtggagtgaa aggcccagca ggtccaaatt catttgtgga agtcaaacgt
    10621 acatcaggta atgtgtttat agaaaatggt agtaaatttg caaaaggcgg aaaataccta
    10681 ggtaaaggtg ttgctggtgt aggatttggt ataggtatgt atgatgacct tgcaaatgat
    10741 gataaaacat ttggagaggc attatcgcat aatggtatga cgcttgcagc tggatcagga
    10801 gcaggagctg ttgtaacagt tggtgcatcg atattattag gtagtaatcc agttgggtgg
    10861 gctgttatag gtggctttgt ggctggaaca ttagtatcaa aagcagcgga ttgggcatat
    10921 caaagcaatt ggaatggtat aaaagataaa acagactggg taggacataa aatagacgat
    10981 agtattgatg ttgttaaaaa atctacagaa aaagccgtag atagtgttgg tgaagctaca
    11041 aaaagtatct ctaatcattt aaaccctatg aaatggagct ggtaaatttt gctttacgaa
    11101 tctaatatta ttaacaaaaa tcccagatat agaattatta aatataaaaa cgactatctg
    11161 atgattgatt tagtaagcac ctggttagtt ttgttctttc cgtttattaa ttggttaata
    11221 ccaaaaaaat atgtccaaat cagcagagaa gagtttgata atttaaatat tgttaaacca
    11281 gttaaaaata aagctttatg gccagtt
//
LOCUS       DAHHYJ010000087        11296 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000087 DAHHYJ010000000
VERSION     DAHHYJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.087"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            303..863
                     /locus_tag="NBK22_001752"
     CDS             303..863
                     /locus_tag="NBK22_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCD2414936.1"
                     /translation="MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTD
                     NALWKNVRDALKDANIIDKTDNENVKVTYKIENGGENTIEGTANLENISTSNNPKINP
                     QNVTKINITRKNSNYPNIDANNTWKKLTEKLKEKNIVNNGDNVSILSTDPKDETVFGK
                     VGEDKSNVSNRYINPKDINEIQITKK"
     gene            1740..2444
                     /gene="tst"
                     /locus_tag="NBK22_001753"
     CDS             1740..2444
                     /gene="tst"
                     /locus_tag="NBK22_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_111772164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic shock syndrome toxin TSST-1"
                     /protein_id="HCD2414937.1"
                     /translation="MNKKLLMNFFIVSPLLLATIATDFTPVPLSSNQIIKTAKASTND
                     NIKDLLDWYSSGSDTFTNSEVLDNSLGSMRIKNTDGSISLIIFPSPYYSPAFTKGEKV
                     DLNTKRTKKSQHTSEGTYIHFQISGVTNTEKLPTPIELPLKVKVHGKDSPLKYWPKFD
                     KKQLAISTLDFEIRHQLTQIHGLYRSSDKTGGYWKITMNDGSTYQSDLSKKFEYNTEK
                     PPINIDEIKTIEAEIN"
     gene            complement(2599..3168)
                     /locus_tag="NBK22_001754"
     CDS             complement(2599..3168)
                     /locus_tag="NBK22_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCD2414938.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYSANSAHVTGCRLLK
                     KPHIKQYIQEQKDKVIDENVLTAKELLHVLTNAAVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELVEVPIKPSDRLKARDMLGKYHKLFTDKHDINGNLPIFINIGEWDGD
                     DEDLDKTVQEVSNTNPNHTVIVDDIPLED"
     gene            complement(3165..3377)
                     /locus_tag="NBK22_001755"
     CDS             complement(3165..3377)
                     /locus_tag="NBK22_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCD2414939.1"
                     /translation="MADDNIDDHIVKNHLEMIVDQVANDKEFYIFDSLIQGRSFKDIS
                     NVLECSEQSVILWYETLLDKIVEVIE"
     gene            complement(3509..4036)
                     /locus_tag="NBK22_001756"
     CDS             complement(3509..4036)
                     /locus_tag="NBK22_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HCD2414940.1"
                     /translation="MKLLKTKNCLYYRNGDNKLSEYQLLTQFNPAFINKKIKMCEFQI
                     ESMYHMSASTTTCDEIMGVVSVSYPIEKLVIKIIETKAGLQNYKNRSINNMALLKKVL
                     NHYTEKEQKKVVKYMRSNGRYKPYNVIERLQVDLYQASIKQRSERQKQRNTAIENSKI
                     ARVNAYHQSSHVKVV"
     gene            complement(4087..4305)
                     /locus_tag="NBK22_001757"
     CDS             complement(4087..4305)
                     /locus_tag="NBK22_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid morphogenesis B protein"
                     /protein_id="HCD2414941.1"
                     /translation="METKYELNNTKKVANAFGLNEEDTNLLINAVDLDIKNNMQEISS
                     ELQQSEQSKQKQYGTTLQNLAKQNRIIK"
     gene            complement(4323..4901)
                     /locus_tag="NBK22_001758"
     CDS             complement(4323..4901)
                     /locus_tag="NBK22_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD2414942.1"
                     /translation="MKTESYFKEYNQFVIDQQKAIQELEQERNALESKIKIDKSTYKQ
                     LIMDGQDDKADNLYQATDADEKKLKALNKRLETKKSVSKEVKYQKTIELLKHQSELSS
                     LYESEKQSALGKLKKVVDAYNEIIDEIEDINDRYEDEHQQYASIYSQEQLYDDKEARE
                     ALNGYFRENIFTSYINGNDLPYEHNNKLFLKR"
     gene            complement(4913..5254)
                     /locus_tag="NBK22_001759"
     CDS             complement(4913..5254)
                     /locus_tag="NBK22_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD2414943.1"
                     /translation="MQSIAEKETYHLPTEHLQVFNVIKNTSNKYITKTKILNQLGYEY
                     NSSNERWLRRVINSLVYDYGYPIGCSYKPSERGYYIITTEQEKQQAMRSIKKLADGSM
                     KRYEALKRIKV"
     gene            complement(5703..6344)
                     /locus_tag="NBK22_001760"
     CDS             complement(5703..6344)
                     /locus_tag="NBK22_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012990921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD2414944.1"
                     /translation="MNIETIVNQFETRAGTLLRYYTGLLEHSKVQPCCFKLYNDPFDM
                     VYVTMNGKLFGHVYIKDCKVRQSFELASPKHTEGLIRSIEGHYVGYELHDGKQLSISD
                     MMASHLFEDEYFMYGLQTYAESNNSDVFEYLKNGFDTDTLEGIQSSNTDVIANIEMLY
                     QLATGINEPAPELVEGLRLVTEFVQDENATQEDYKALESKLNDLKESYYSLSK"
     gene            complement(6341..6721)
                     /locus_tag="NBK22_001761"
     CDS             complement(6341..6721)
                     /locus_tag="NBK22_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414945.1"
                     /translation="MDKEQLKKYIYDYVKEYKEIPIYQLEDLFKELNHDYIGRTSITH
                     DKDENIVFWSGWNKITMFALIELVKSEQLDLVYRGSFVMRYLLDGRVPNLPLAICYPE
                     DGQQTDVPSWVPMVLRINKEEKIK"
     gene            complement(7051..8751)
                     /locus_tag="NBK22_001762"
     CDS             complement(7051..8751)
                     /locus_tag="NBK22_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCD2414946.1"
                     /translation="METGKSDVLDKIEKINKKDSALQEIIPKGYEIEHHQCGVALYQL
                     IPSKKEGEPDKKVFITNTIPQITERFEDIESNEVSFNMLFYDNKTPVNIAVSAEEISD
                     SRQLLKLVNKKLDVTSSTSTKLVDYINVSKRYNPPLNVKVATRLGHVKGYFIYPYQEV
                     MKDSNIKLFSNDKGFQKLIDSFQSKGTLEGYSKKVFGQIKDLPMVMVMLYASLGSVLL
                     REFGLQPFIVEISGSTSTGKTFTLNLVSSVWGTSDLITTWSSTQNSIESMASFLNSFP
                     MFKDDTRNTHPKFVTSATYNFSSGESKSRSNINLTLNAKKEWRNILISTGESSIANMA
                     DEKAGVSARVVTLQDQPYPDNFDFTTLDKSFRENYGTLGLAFIKQYESKKDVYKNAFE
                     SYQRYFNQKGSNEIMQRLGRAFALLQVTGEVLNDIDGFEHDHFKIIEQAYDSMVKNNK
                     TIDKPKQLLEELLQYLDANRNNIAGDGYSSVKNGDIKAIYKRDYLCILGQTVYDKLGH
                     EMQTITGQWDKKGYLITSKDRIQKEVRFNSQKNRGYAIKDEIIKELGFDFSNSHNPYN
                     "
     gene            complement(8765..9634)
                     /locus_tag="NBK22_001763"
     CDS             complement(8765..9634)
                     /locus_tag="NBK22_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001667297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HCD2414947.1"
                     /translation="MNEIKLEYDTHVSVVHYESLDSRSFKSFSKPKWSKLINKLSVPI
                     EANYKYARGVAVYGDIKNGANDHGEIIKKHRNDVNVVYRDVIVLDYDEINDLKQLHEA
                     ISSALSNVAWFWHTSYSHRTEQARIRLYIPLNERISADDYRKYSKVLANKIGHKVDEG
                     SYQPSRCFALPVIQKGHIFIKRVNDCPIIDVDMLEQWSKELEQSNASPNVIGYTRRDS
                     AYWRELSFGTTEGNRNNALASLVGHLLRCRVNDYIVYSYALLWGQFACKPPMKEQEIN
                     ATFQSILNKHYNN"
     gene            complement(9701..10018)
                     /locus_tag="NBK22_001764"
     CDS             complement(9701..10018)
                     /locus_tag="NBK22_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1474 family protein"
                     /protein_id="HCD2414948.1"
                     /translation="MNWEIKDLMCDIEVIKEKINDVAIKHGWFVEDKFVKNELETKQE
                     HINFSASYLEHRIQNEHTVELLQVYLKEFGELIQKFHEIEKASSENFGEESDDAKKLK
                     ITE"
     gene            complement(10021..10230)
                     /locus_tag="NBK22_001765"
     CDS             complement(10021..10230)
                     /locus_tag="NBK22_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD2414949.1"
                     /translation="MNNEQKEVIEHVVYQLELSIMNNFESYEHTEYVDGIEVVSEISR
                     EKHLELIMKWCAQELKNNFQLEKGE"
     gene            complement(10223..10369)
                     /locus_tag="NBK22_001766"
     CDS             complement(10223..10369)
                     /locus_tag="NBK22_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island protein"
                     /protein_id="HCD2414950.1"
                     /translation="MKMYLTYICLVSLLTILLLAISNMYVAFSVYGMMATYGFNLTGG
                     LENE"
     gene            complement(10381..10599)
                     /locus_tag="NBK22_001767"
     CDS             complement(10381..10599)
                     /locus_tag="NBK22_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2414951.1"
                     /translation="MTKLAYPMLYITRKEKGDTQKKVASKLGISPQRYQLKESGKAIF
                     NLNECQILSEMYDMPIDELFSSKIKVGK"
     gene            complement(10635..10838)
                     /locus_tag="NBK22_001768"
     CDS             complement(10635..10838)
                     /locus_tag="NBK22_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD2414952.1"
                     /translation="MNKVLGYRKMLNFTQQQMAKELKISEQSYRNKEKGKTEFKKEEM
                     LTFKNILISKGMTNITLDDIFFN"
     gene            10974..>11296
                     /locus_tag="NBK22_001769"
     CDS             10974..>11296
                     /locus_tag="NBK22_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2414953.1"
                     /translation="MDKKTDIGLRIKSIRLAKGLNLREFGEEISKLTKEKKYISDSIV
                     SRWEKGVSIPNAKRLKAIAEYGNVSINFLLYGNEVSYEDIYQNIQSVNMKNNIQDKLI
                     DFIVN"
BASE COUNT         3549 a         2048 c         1486 g         4213 t
ORIGIN      
        1 tagagatata ataataggca agtaccgaag tacttgccta aatatcaaca agattaacat
       61 gtgaataata atggaaataa aaagtcagcc cgaaggctaa cttacgaata gatgaaaatt
      121 tgaacacatt gctgtgtcta aaatgattat agcataaatg acgaatactt ctagctcaaa
      181 attattatat tttaatgata aaattttatg gatttgttaa taattattta attgatttac
      241 ataaataata attgtaaaat tactttgtaa ttgattgcaa ataagttata ggagaaaata
      301 aaatgaataa aaaactatta acaaaaacat tgatagcaag tgctttagtt ttaacaacag
      361 taggttcagg ttttcattct tcttcaaatt ataatggtat taataacgtt gcaaaagctg
      421 agcaaacgac agataacgca ttgtggaaaa atgtaagaga cgctttaaaa gacgcgaata
      481 ttatcgataa aacagataat gaaaatgtca aggttacgta taaaatagaa aatggtggag
      541 aaaataccat agaaggaaca gctaatttag aaaatattag tacttcaaac aatcctaaaa
      601 taaaccctca aaatgttaca aaaattaata taactagaaa aaattcgaac tatcctaata
      661 ttgatgctaa taatacatgg aaaaaattaa cagaaaaatt gaaagaaaaa aatatagtga
      721 acaacggcga caatgtttca atcttaagta cagaccctaa agatgagact gtattcggta
      781 aagtaggaga agataaatca aacgtaagca atagatacat caatcctaaa gatataaatg
      841 aaattcaaat cactaaaaaa taactttttt cgagaggcag attactcctg cctcttttct
      901 tttgaacagt gataatcttc tggatctatg taacactcaa ttacttcaga ttctttacct
      961 ttaacttcct ttaattactt tctctctatc tcctcaaaaa gttgtgcttt ttcatttgtg
     1021 attggagttg ggcgtttttt catcgcgttg tttcaattcc tttttaaggt attctaattc
     1081 tctcctagtc atatcaattg tttttttact tctcaccttt agtgaaatac tcttatcctt
     1141 tctcttcttg cggtaatgtt gctaattagt ataaaataca tgcgcccata tattccaatg
     1201 gtaggacatt taattctgga ttttcagcta ttttcataaa tctattatct gataatttgc
     1261 ttaatccaat tttcaagcca tagcctaaat tccccatcca ctaagtcatt ttgtttcata
     1321 tggttttaat ctacggccaa tctcaaagat agattgacca gcgatgttta aagtcatatt
     1381 tcacggatcc acatttacga taaacatatc tagttacaca atattatccc ttactgcaac
     1441 acaggacgtt tctcagcgta aaaaacgcca ctagaaagtg actttaaaga atataactaa
     1501 ttcaaactta tattaattaa tattctttaa atgaccactc acactttgtt ttttgctatt
     1561 tgtaacttta aaattttgtt taaatctata tttttttgat atagctccct atgtaacaaa
     1621 cactttttaa ttaatatata tttaaacaat aatttagaga tggttaattg aatcatttaa
     1681 ataatattta tacattctat atgtaaacgt ttacacattt gaatgaagga gaattaaaaa
     1741 tgaataaaaa attactaatg aattttttta tcgtaagccc tttgttgctt gcgacaatcg
     1801 ctacagattt tacccctgtt cccttatcat ctaatcaaat aatcaaaact gcaaaagcat
     1861 ctacaaacga taatataaag gatttgctag actggtatag tagtgggtct gacactttta
     1921 caaatagtga agttttagat aattccttag gatctatgcg tataaaaaac acagatggca
     1981 gcatcagcct tataattttt ccgagtcctt attatagccc tgcttttaca aaaggggaaa
     2041 aagttgactt aaacacaaaa agaactaaaa aaagccaaca tactagcgaa ggaacttata
     2101 tccatttcca aataagtggc gttacaaata ctgaaaaatt acctactcca atagaactac
     2161 ctttaaaagt taaggttcat ggtaaagata gccccttaaa gtattggcca aagttcgata
     2221 aaaaacaatt agctatatca actttagact ttgaaattcg tcatcagcta actcaaatac
     2281 atggattata tcgttcaagc gataaaacgg gtggttattg gaaaataaca atgaatgacg
     2341 gatccacata tcaaagtgat ttatctaaaa agtttgaata caatactgaa aaaccaccta
     2401 taaatattga tgaaataaaa actatagaag cagaaattaa ttaatttacc actttttctg
     2461 taataattat taataaaggg agtccgttaa ttataataag cgttctccct tttatcttta
     2521 taacgcttta atataaagct agggtgaatt agtaaactaa tcctcactaa ctcaatatga
     2581 taatcgtttt tactgtaatc aatcctctaa cggtatatca tccacaatca cagtatgatt
     2641 aggattagtg ttagatacct cttgtaccgt cttatctaaa tcttcatcat cgccatccca
     2701 ttcaccaata ttaatgaata taggcaaatt cccgttaata tcatgcttat ctgtaaataa
     2761 cttatggtat ttacccaaca tatcacgagc ttttaaacga tcacttggct taattggcac
     2821 ctctaccagt tcaacatgtt cgttatagac taactgtact ttgccacttt gtggattctc
     2881 tttatattct ccacgtttga ccacaacttc tttcgtttct gtttcatcac cgactgccgc
     2941 attcgtaagc acatgtagta actcttttgc ggttaataca ttctcatcta taaccttatc
     3001 tttttgttct tgtatatatt gcttgatgtg tggcttcttt aataacctac atcctgtcac
     3061 atgtgcacta tttgcgctat agcctgcttt tatggcactt tgtgttacat taagtgttct
     3121 tatatactca ttcacaaaac gcgcttgttt tgccgttaac tcactcattc tatcacctcc
     3181 acaattttat ctaataaggt ttcataccat aatattacag attgttctga acactctaag
     3241 acattgctaa tatctttaaa actacgtcct tgtattaaag aatcgaaaat ataaaactct
     3301 ttatcattag ctacttggtc aacaatcatt tctaagtgat tctttacaat atgatcatca
     3361 atgttatcgt ctgccatcca tttattagaa ttttcatcac ctattgaaaa gaattcatcg
     3421 gtatttattt catcatctat caacacatca cttttagttc gcttatgata atcacaaacg
     3481 aagtctttta tttgcttttt atccattgtt acaccacttt tacatgtgaa gattgatgat
     3541 aagcatttac tcgtgcaatc ttgctatttt caattgctgt atttctttgt ttttgacgtt
     3601 ctgaacgttg tttaatactt gcttgataca aatcaacctg taaacgttca atgacgttgt
     3661 agggcttata tcgtccattt gaacgcatat attttacaac tttcttctgc tctttttctg
     3721 tataatgatt tagtaccttt ttcaacaacg ccatattatt tatagatcta tttttatagt
     3781 tttgtaaccc tgcttttgtt tcaataattt tgataactaa tttttcaatc ggatatgaga
     3841 cagacacgac ccccattatt tcatcacatg ttgtggtcga cgcactcata tggtacatac
     3901 tttcaatttg gaattcacac atcttaattt ttttattaat aaatgctggg ttaaattgcg
     3961 ttaatagttg atactcagat agtttattgt cgccattacg ataatataaa caattcttcg
     4021 ttttaagcag tttcatttat tcacccctat aaacagagcc tacccgaatt ggataggcaa
     4081 tcattgctat ttaataatcc tgttttgctt agctaaattt tgtagcgttg taccatattg
     4141 cttttgctta gactgttctg attgttgtaa ctcacttgaa atctcctgca tattgttttt
     4201 aatatccaaa tcaactgcat ttattaatag atttgtatct tcttcattta aaccaaatgc
     4261 atttgcgacc tttttagtat tatttaactc gtattttgtt tccatttaat taccctttct
     4321 ttttaacgtt ttaaaaacaa cttgttattg tgttcgtatg gcaaatcatt accattaata
     4381 tatgatgtaa atatattttc tctaaagtag ccattcaatg cttccctagc ctctttatca
     4441 tcatataatt gttcttgact ataaatactc gcatattgct gatgctcatc ttcatatcta
     4501 tcattaatat cttctatttc atcaatgatc tcattatatg catcgactac tttttttaat
     4561 ttacctaaag ctgattgctt ttctgattcg tataatgatg acaactcgct ttgatgtttt
     4621 aataattcaa ttgtcttttg atatttaact tctttcgaca cacttttctt tgtctctaag
     4681 cgtttattaa gtgcttttag tttcttttca tcagcatctg ttgcttgata taggttatct
     4741 gctttatcat cttgtccatc catgattaac tgtttatatg tggacttatc tatctttatt
     4801 ttactctcca atgcattacg ctcttgttcc aattcttgta tagccttttg ttgatctatt
     4861 acaaattggt tgtattcttt aaagtacgat tcagttttca tttttatccc ctttacactt
     4921 taattcgttt caaagcttca tagcgtttca tactgccatc agctaatttc ttaatacttc
     4981 tcatcgcttg ttgcttttct tgttctgtcg taatgatgta ataaccacgt tcactaggtt
     5041 tataactgca tccgatagga tagccataat catatactaa tgaattgatt actcttcgta
     5101 accatcgttc attgcttgaa ttatattcat atcccaattg atttaagatt ttagttttag
     5161 taatatactt attggacgta ttttttatca cattgaaaac ttgcaggtgt tcggtgggta
     5221 aatgatacgt ctctttttct gcgatacttt gcatttctac acctctttct tttaattatt
     5281 tcatacctaa attataccat ttttacaggt ctaaaacaaa cttacgttcg ctttatagcg
     5341 cgtttatcaa ttgtttagct tatcatatat aacacttata aaatcatgtt ataaacttaa
     5401 cgttaggctt ttcacattaa cctaatatag aacttaagtt cggtaaaata acacgaacaa
     5461 acagcgaaca aacttaactt ttaggcctat gccaaaaaca caaactttag cttgtattag
     5521 cgttaacaaa gttcgcacac cttgcacaaa tcttgccatt ttttcaattc tcaaagactg
     5581 tataccttcc gattttaaaa gccaacacct tccgaaaacc ttaccatttt aaactgctat
     5641 acctcgtata aaatcgtagt attttattag gagccacaca ctacatgtga cccctcataa
     5701 cattatttac tcaagctata gtaagactct tttagatcat tcaatttact ttctaaagcc
     5761 ttgtaatcct cttgtgtcgc attctcatct tgtacaaact cagttactaa tctcaacccc
     5821 tcaactaact ctggtgctgg ttcattgatt cccgtagcta actgatacaa catttcaata
     5881 tttgctatca catcagtatt actcgattga atgccctcaa gtgtatcggt atcaaatcca
     5941 ttttttaggt attcaaacac atcactatta tttgattctg catatgtttg taatccatac
     6001 ataaaatact catcttcaaa caaatgacta gccatcatat cactaataga aagctgtttg
     6061 ccatcatgta attcataacc tacataatga ccttctatgc ttcttataag cccctcagtg
     6121 tgcttaggtg acgctaattc aaatgattgc cttactttac aatctttaat atatacatga
     6181 ccgaataact tcccattcat cgtcacataa accatatcaa atggatcatt gtataactta
     6241 aagcaacatg gttgcacttt actatgttct aataatcctg tgtagtacct tagtaacgtg
     6301 cctgctcgtg tttcaaattg gtttacgata gtttctatgt tcatttgatt ttctcctctt
     6361 tatttattct taataccata ggcacccatg agggcacgtc agtttgttgt ccatcttctg
     6421 gataacaaat agctaatggt aaattaggaa ctctaccatc caacaaataa cgcattacaa
     6481 aactacctct atacactaaa tcaagttgtt cacttttaac taattcaatc aacgcaaaca
     6541 ttgtaatttt gttccatcca ctccaaaaca caatattctc atccttatcg tgtgtgatac
     6601 tagttctccc tatatagtcg tgattcagtt ctttaaacaa atcttctaac tgatatatcg
     6661 gtatctcctt atattctttt acataatcgt atatatactt tttaagttgt tctttatcca
     6721 tgtgtaacct ccagcattaa tttgatgttt tttattttag taggggacta gtccccagtg
     6781 agtccccacc ctaattattt attggggact gtttcacccg ttgatataac tgattttgtt
     6841 agaaatagtc cccatagtca ccatatttat aaacgatggt agctatatat taaattacac
     6901 tcaatgttct ttttgatcat tttatataaa cactgttttt tattggggac tttggggact
     6961 aatctctcta aagtattgcg gtaatagtgt ttattaagtc cccaatcatt cctttttagt
     7021 ggggactgtt tggagacttt ggggactttt ttaattatat ggattatgtg aattagaaaa
     7081 atcaaaccct aattctttta taatctcatc tttaatggca taccctctat tcttttgaga
     7141 gttaaatcta acttcttttt gtattctgtc tttactagtt attaaataac cctttttgtc
     7201 ccattgtcct gtgatagttt gcatttcatg acctaacttg tcgtatacag tttgacctaa
     7261 tatacataaa taatcacgtt tatatatagc tttgatgtca ccatttttga ctgaactata
     7321 gccatcacca gcgatattat ttctatttgc atctaaatat tgtaatagtt cctctaacag
     7381 ttgcttaggt ttatcaattg tcttattgtt tttaaccatg ctgtcatagg cttgttcaat
     7441 aattttaaaa tggtcatgtt caaatccatc aatatcattc aaaacctcac cggtaacttg
     7501 tagtaacgca aaggcacgtc ctaaacgttg catgatttca ttactacctt tttgattaaa
     7561 ataccgttga tagctttcaa aagcgttctt atacacgtct tttttagact catattgttt
     7621 aataaatgcc aaccctaacg ttccatagtt ctccctaaac gatttgtcta atgtggtaaa
     7681 atcaaaatta tctggatatg gttgatcttg tagtgtaact acacgtgctg atacacccgc
     7741 tttttcatca gccatatttg cgatagatga ttcaccagta gaaattaaaa tatttcgcca
     7801 ttctttttta gcatttagcg ttaaattaat attacttctt gatttgcttt cgccactaga
     7861 aaagttatat gtggcactgg taacaaactt aggatgtgta ttacgtgtat catctttaaa
     7921 cattggaaat gagttcaaaa atgatgccat tgattcaata ctattttgag tagaactcca
     7981 tgtcgtaata aggtcactgg ttccccaaac acttgatact aagttgagtg tgaatgtttt
     8041 acctgtggat gtactacctg atatttctac aataaagggt tgtaatccaa attctcttaa
     8101 taaaactgag cctaaagatg catataacat aaccattacc attggtagat cttttatttg
     8161 accaaacact tttttagagt aaccttctaa tgttcctttg ctttgaaaag agtctattaa
     8221 cttttgaaat cctttatcat tactaaacaa cttgatattg ctgtctttca tcacttcttg
     8281 ataaggataa ataaaataac ctttcacatg tcccaagcgc gttgcaactt taacatttaa
     8341 tggtggatta taccgtttag atacattaat atagtcaaca agtttagtag atgtcgacga
     8401 tgttacatct agctttttat taaccaattt caagagttga cgactatctg aaatttcttc
     8461 ggcgctcact gctatattta caggcgtttt attgtcatag aaaagcatat taaagctgac
     8521 ttcgttactc tcaatatctt caaagcgttc agtaatttga gggattgtat ttgtgataaa
     8581 aaccttttta tctggttcgc cttctttttt acttggtata agttgatata aagcaacacc
     8641 gcattgatga tgttcaattt catagccttt cggtataatt tcttgtaagg cactatcttt
     8701 tttattaatt ttttcaattt tatcaagtac atcactttta cctgtttcca tacaaagccc
     8761 ctttctaatt gttatagtgt ttatttaata tcgattgaaa agttgcgttg atttcttgtt
     8821 ctttcatagg tggtttacat gcgaattgcc cccataataa agcatatgaa tacacaatat
     8881 aatcattaac gcgacatctt aataaatgcc ccactaagct agctagtgca ttgttacgat
     8941 tgccttcggt tgttccaaag cttaactcac gccagtacgc actatcacgt cgcgtgtacc
     9001 ctataacatt aggactagca tttgattgtt caagttcctt cgaccactgt tcgagcatat
     9061 caacatcgat aattggacag tcattcactc gcttaataaa tatgtgccct ttttgaataa
     9121 ctggtaatgc aaaacatcta cttggctgat atgaaccttc atcaacttta tgaccaattt
     9181 tgtttgctaa tacctttgaa tatttacgat aatcatctgc acttattcgc tcatttagag
     9241 ggatatacag gcgtattcta gcttgttcag ttctgtgcga gtaacttgtg tgccaaaacc
     9301 atgcaacatt gcttaaagct gagctgattg cttcatgtaa ttgctttaaa tcatttattt
     9361 catcgtaatc aagtacaatc acatctctgt atacgacatt aacgtcattg cgatgctttt
     9421 tgataatttc accatgatca tttgcaccgt ttttaatatc accgtaaaca gcaacaccac
     9481 gtgcatactt ataatttgct tctataggca cagacagttt attaattaac ttactccatt
     9541 taggttttga aaagctctta aatgaacgtg agtctaaact ttcataatgt accactgaaa
     9601 catgtgtgtc atattctaat ttaatttcat tcattttttg tacctcttaa tgaaacaaca
     9661 gagcaaagat gttataataa aaatagttat tttttattaa ttactctgta atttttaatt
     9721 tctttgcgtc atctgattcc tcgccaaagt tctcagatga tgctttttct atttcatgaa
     9781 acttttgtat aagttcaccg aattctttta agtacacctg taataactca actgtatgtt
     9841 cattttgtat acgatgttct aaatagctag cagaaaaatt aatatgttcc tgttttgttt
     9901 ctaattcatt tttgacaaat ttatcttcaa caaaccaacc atgtttgata gctacatcat
     9961 tgattttttc ttttatcact tcaatatcac acatcaaatc tttaatttcc caattcatat
    10021 ttattctcct ttttctaatt gaaaattatt ctttaattct tgtgcgcacc atttcattat
    10081 caattctaag tgcttttcac gactgatctc tgaaaccact tcaataccat caacatattc
    10141 cgtgtgttca taactttcaa aattattcat gatacttaac tcaagttgat aaaccacgtg
    10201 ttctattact tctttttgtt cattattcat tttctaatcc tcctgttaaa ttaaatccat
    10261 aagttgccat catgccgtac acactaaaag cgacatacat gttagatatt gctagtaata
    10321 aaattgttaa caatgaaact aagcaaatat aagttaagta cattttcatt gccttgcctc
    10381 ctattttcct actttaattt ttgatgaaaa caactcatca attggcatat catacatttc
    10441 tgaaagaatc tgacactcat ttaaattaaa tattgccttg ccactttcct ttaactggta
    10501 gcgttgtgga ctaataccaa gtttgctagc aactttcttt tgtgtgtcac ccttttcttt
    10561 tctagtaatg tataacattg gataagctag ttttgtcatt tgaccacctc ttttacgtag
    10621 ttttacgtcg gattttaatt aaaaaaaata tcatctaaag ttatattggt cattcccttt
    10681 gaaataagta tgtttttaaa agttaacatt tcttctttct taaattccgt ttttcctttt
    10741 tctttatttc tgtatgactg ttctgatatt ttaagctctt ttgccatttg ttgttgtgtg
    10801 aagttcaaca ttttcctata acccagtact ttattcactt attatcacct cttccctaga
    10861 cgtaagttct cgtctgtatg tataatataa cagacacgaa aactaaaagc aacacaaaaa
    10921 cgtcggttta cgaaagtttt tatggatatt ctcattagat aggaagtgaa aatatggata
    10981 aaaaaacaga tattggttta cgcatcaaaa gtatcagact tgctaaagga ttgaatttaa
    11041 gagaatttgg cgaagaaata tcaaaattaa caaaagaaaa aaaatatatt tcggatagta
    11101 tagttagtcg atgggaaaaa ggggtgtcaa tacccaatgc caaaaggtta aaagctattg
    11161 cagaatatgg taatgtgtct attaattttt tactatatgg aaatgaggtt tcatacgaag
    11221 atatttatca aaacatacaa tcagtgaata tgaaaaacaa tattcaagac aagttaattg
    11281 attttatcgt taatta
//
LOCUS       DAHHYJ010000088        11136 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000088 DAHHYJ010000000
VERSION     DAHHYJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.088"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            222..1910
                     /gene="ilvD"
                     /locus_tag="NBK22_001770"
     CDS             222..1910
                     /gene="ilvD"
                     /locus_tag="NBK22_001770"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCD2414954.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            1938..3707
                     /gene="ilvB"
                     /locus_tag="NBK22_001771"
     CDS             1938..3707
                     /gene="ilvB"
                     /locus_tag="NBK22_001771"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCD2414955.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQ
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVTPMVPSGKSNHEMEGL"
     gene            3707..3961
                     /locus_tag="NBK22_001772"
     CDS             3707..3961
                     /locus_tag="NBK22_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCD2414956.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            4098..5102
                     /gene="ilvC"
                     /locus_tag="NBK22_001773"
     CDS             4098..5102
                     /gene="ilvC"
                     /locus_tag="NBK22_001773"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCD2414957.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQDASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            5132..6661
                     /locus_tag="NBK22_001774"
     CDS             5132..6661
                     /locus_tag="NBK22_001774"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCD2414958.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDHYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEGHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            6664..7710
                     /gene="leuB"
                     /locus_tag="NBK22_001775"
     CDS             6664..7710
                     /gene="leuB"
                     /locus_tag="NBK22_001775"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCD2414959.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVN
                     IRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            7724..9094
                     /gene="leuC"
                     /locus_tag="NBK22_001776"
     CDS             7724..9094
                     /gene="leuC"
                     /locus_tag="NBK22_001776"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCD2414960.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVI"
     gene            9095..9667
                     /gene="leuD"
                     /locus_tag="NBK22_001777"
     CDS             9095..9667
                     /gene="leuD"
                     /locus_tag="NBK22_001777"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCD2414961.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGLSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            9682..10950
                     /gene="ilvA"
                     /locus_tag="NBK22_001778"
     CDS             9682..10950
                     /gene="ilvA"
                     /locus_tag="NBK22_001778"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCD2414962.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQSKSYNVNFDYLFAAIGGGGLISGISTYFKTYSPTTKIIGVEPSGA
                     SSMYESVVVNNQVVTLPNIDKFVDGASVARVGDITFEIAKENVDDYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDQIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDHD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
BASE COUNT         3747 a         1838 c         2153 g         3398 t
ORIGIN      
        1 gaacatcaac cgacaacttc aaatattatt attttattat actctttagg actcgaacgt
       61 tagtaaatat ttactaaacg ctttaagtcc tatttctgtt tgaatgggac ttgtaaacgt
      121 cccaataata ttgggacgtt tttttatgtt ttatctttca attacttatt tttattacta
      181 taaaacatga ttaatcatta aaatttacgg gggaatttac tatgcgaagc gacatgatca
      241 aaaaaggaga tcaccaagca ccagcaagaa gtcttttaca tgccacgggc gcgctaaaaa
      301 gtccaactga tatgaacaaa ccatttgtag ctatttgtaa ctcttatatt gatattgttc
      361 ctggacatgt tcacttgaga gagcttgcag atatagctaa agaagcaatt agagaagccg
      421 gtgccattcc atttgaattc aatacaattg gtgttgatga tggaatagct atgggacata
      481 tcggaatgcg atattctcta ccatcacgtg aaattattgc agatgcagct gaaactgtaa
      541 ttaacgctca ttggtttgac ggcgtatttt acattcctaa ttgtgacaag attacacccg
      601 gtatgatttt agcagccatg aggacaaacg taccagctat cttttgctct ggtggaccaa
      661 tgaaagctgg cttatctgca catggaaaag cattaacact ttcatcaatg tttgaagcag
      721 tcggcgcatt taaagaagga tcgatttcta aagaagaatt tttagatatg gaacaaaatg
      781 cctgccctac ttgtggttca tgtgctggga tgtttactgc aaattcaatg aactgtttga
      841 tggaagtttt aggtctagca ttaccataca acggtactgc acttgcagtc agtgatcagc
      901 gacgagaaat gattcgccaa gcagctttta aattagttga aaatattaaa aatgatttaa
      961 aaccacgtga tatcgttact cgcgaagcaa ttgatgatgc atttgcactt gatatggcta
     1021 tgggtggttc aacaaacacg gtactgcata cgttagccat tgccaatgaa gctggtattg
     1081 attatgactt agagcgcatt aatgctattg ccaaacgcac gccatattta tcaaaaatag
     1141 cacctagttc atcgtattca atgcatgatg tgcatgaagc tggtggcgtc ccagcaatta
     1201 ttaatgaatt gatgaagaaa gatggcacgt tacacccaga tagaatcaca gttactggca
     1261 aaacgttacg tgaaaataac gaaggcaaag aaattaagaa ctttgatgtc attcaccctc
     1321 ttgatgcacc atatgatgca caaggcggtt tatctatctt atttggtaat atcgccccta
     1381 aaggcgcagt tattaaagtt ggcggcgttg atccatctat caaaacattt actgggaaag
     1441 caatttgttt caattcgcat gatgaagctg ttgaagcaat agacaatcgt accgttcgtg
     1501 caggccacgt cgttgtcatt agatatgaag gacctaaagg tggaccaggt atgcctgaaa
     1561 tgttagcacc tacttcctct attgttggtc gcggcttagg taaagatgtt gcattaatta
     1621 ctgatgggcg tttttccggt gccacaagag gtattgcagt tggtcatatt tcccctgaag
     1681 ctgcatctgg tggaccaatt gccttaattg aagatggtga tgagattact attgatttaa
     1741 caaatcgtac attaaacgta aaccagcctg aagatgttct agcgcgtcgc cgagaatctt
     1801 taacaccatt taaagcgaaa gtaaaaacag gttatctagc tcgttatact gccctagtaa
     1861 ctagcgcaaa tacaggtggc gtcatgcaag tccctgagaa tttaatttaa tttattttta
     1921 tattggagat ggttaaaatg tctaaaactc aacatgaagt aaaccaaaat attgaccctt
     1981 taaaaatggc tgaatcactt gaacctgaac aactaaatga aaaaacttta aatgatatgc
     2041 gttcaggatc agaagtgcta gtagaagctc tacttaaaga aaatgtggat tatttattcg
     2101 gttatcctgg tggtgccgta ctacctttat atgacacgtt ttatgatggt aaaatcaaac
     2161 atattttagc aagacacgaa caaggtgctg ttcatgctgc agaaggttat gcacgtgtat
     2221 ctggtaaaac tggcgtcgtt gtagttacaa gcggtccagg tgcaactaat gtaatgacag
     2281 gtattacgga tgcacattgc gactctttac ctctagttgt attcactgga caagttgcta
     2341 caccaggcat tggtaaagat gcattccaag aagcggatat tctatctatg acttcaccaa
     2401 ttacaaaaca aaattatcaa gtgaaacgtg ttgaagatat ccctaaaatc gtacacgaag
     2461 ctttccatgt agctaattct ggacgcaaag gtcctgtagt gattgatttt ccaaaagata
     2521 tgggtgtttt agctacaaat gtggatttat gcgacgaaat caatattcca ggttatgaag
     2581 ttgttacaga accagaaaat aaagacattg acactttcat ctcactttta aaagaagcga
     2641 aaaagcctgt cgtattagcc ggcgcaggta ttaatcaatc aaaatcaaat caattattaa
     2701 cacagtttgt taataaacat caaatcccaa cagttactac tttactaggt ctaggtgctg
     2761 taccttatga ggatacacta tttttaggta tgggaggaat gcatggttct tatgctagta
     2821 acatggcatt aactgagtgt gatttactca ttaatttagg tagccgcttc gatgatagat
     2881 tagcaagcaa acctgatgcc tttgcaccta acgccaaaat tgtacatgta gatattgatc
     2941 cttcagaaat caataaagtt attcatgtag atttaggtat tattgcagac tgtaaaagat
     3001 ttttagaatg tttaaatgat aaaaatgttg agactataga acacagtgac tgggttaaac
     3061 attgtcaaaa taataagcag aaacacccat ttaaacttgg tgaagaagat caagtatttt
     3121 gtaagccaca acaaacaatc gaatatatcg gcaaaattac aaatggtgaa gcaattgtta
     3181 ctacagacgt gggacaacat caaatgtggg cagctcaatt ttatccattt aaaaatcacg
     3241 gacaatgggt tacaagcggt ggtttaggaa caatgggatt cggtattcct tcgtcaattg
     3301 gtgccaaatt agctaatcct gataaaacag tcgtatgttt cgtcggtgac ggtggtttcc
     3361 aaatgacaaa ccaagaaatg gcacttttac ccgaatatgg tttagatgtc aaaatcgtac
     3421 taatcaataa tggaacatta ggtatggtta aacaatggca agataagttc tttaatcaac
     3481 gcttctcaca ctcagtattt aatggtcaac ctgattttat gaaaatggca gaagcatatg
     3541 gcgtcaaagg tttcttaatc gataagccag aacaactgga agaacaatta gatgcagcgt
     3601 ttgcttatca aggaccagct ttaattgagg ttcgtatttc ccctactgaa gctgtaaccc
     3661 caatggttcc gagtggcaaa tcaaatcatg aaatggaggg cttataatga caagaattct
     3721 taaattacaa gttgcggatc aagtcagcac gctaaatcga attacaagtg cttttgttcg
     3781 cctacaatat aatatcgata cattacatgt tacacattct gaacaacctg ggatttctaa
     3841 catggaaatt caagtcgata ttcaagatga tacatcactt catatattaa ttaaaaaatt
     3901 aaaacaacaa attaatgttt taacggttga atgctacgac cttgttgata acgaagctta
     3961 attttaagac aaaggcaatg atgcgctaat tagttataga tatatcatag gctgctagtt
     4021 aacatctgcc actattacaa agttatattt cagaattttc gaaacacaaa atatttaatt
     4081 atttggagga atttattatg acaacagttt attatgatca agatgtaaaa acggacgctt
     4141 tacaaggcaa aaaaattgca gtagtaggtt atggatcaca aggtcacgcg catgcacaaa
     4201 acttaaaaga caatggatat gatgtagtca tcggcattcg cccaggtcgt tcttttgaca
     4261 aagctaaaga agatggattt gatgtgttcc ctgttgcaga agcagttaag caagctgatg
     4321 taattatggt gctattacct gatgaaattc aaggtgatgt atacaaaaac gaaattgaac
     4381 caaatttaga aaaacataat gcgcttgcat ttgctcatgg ctttaacatt cattttggtg
     4441 ttattcaacc accagctgat gttgatgtat ttttagtagc tcctaaagga ccgggtcatt
     4501 tagttagacg tacatttgtt gaaggttctg ctgtaccatc actatttggt attcaacaag
     4561 acgcttcagg tcaagcacgt aatattgctt taagttatgc aaaaggtatt ggtgcaactc
     4621 gtgcaggtgt tattgaaaca acatttaaag aagaaactga gacagattta tttggtgaac
     4681 aagcagtact ttgcggtggt gtatcgaaat taattcaaag tggctttgaa acattagtag
     4741 aagcgggtta tcaaccagaa ttagcttatt ttgaagtatt acatgaaatg aaattaatcg
     4801 ttgatttgat gtatgaaggc ggtatggaaa atgtacgtta ctcaatttca aatactgctg
     4861 aatttggtga ctatgtttca ggaccacgtg ttatcacacc agatgttaaa gaaaatatga
     4921 aagctgtatt aactgatatc caaaatggta acttcagtaa tcgctttatc gaagacaata
     4981 aaaatggatt caaagaattt tataaattac gcgaagaaca acatggtcat caaattgaaa
     5041 aagttggtcg tgaattacgc gaaatgatgc cttttattaa atctaaaagc attgaaaaat
     5101 aagatagacc tacaatgagg agttgttaaa tatgagtagt catattcaaa tttttgatac
     5161 gacactaaga gacggtgaac aaacaccagg agtgaatttt acttttgatg aacgcttgcg
     5221 tattgcattg caattagaaa aatggggtgt agatgttatt gaagctggat ttcctgcttc
     5281 aagtacaggt agctttaaat ctgttcaagc aattgcacaa acattaacaa caacggctgt
     5341 atgtggttta gctagatgta aaaaatctga catcgatgct gtatatgaag caacaaaaga
     5401 tgcagcgaag ccggtcgtgc atgtttttat agcaacatca cctattcatc ttgaacataa
     5461 acttaaaatg tctcaagaag acgttttagc atctattaaa gaacatgtca catacgcgaa
     5521 acaattattt gacgttgttc aattttcacc tgaagatgca acgcgtactg aattaccatt
     5581 cttagtgaaa tgtgtacaaa ctgccgttga cgctggagct acagttatta atattcctga
     5641 tacagtcggc tacagttacc atgatgaata tgcacatatt ttcaaaacct taacagaatc
     5701 tgtaacatct tcaaatgaaa ttatttatag tgctcattgc catgacgatt taggaatggc
     5761 tgtttcaaat agtttagctg caattgaagg cggtgcgaga cgaattgaag gcactgtaaa
     5821 tggtattggt gaacgagcag gtaatgcagc acttgaagaa gtcgcgcttg cactatacgt
     5881 tcgaaatgat cattatggtg ctcaaactgc tcttaatctc gaagaaacta aaaaaacatc
     5941 ggatttaatt tcaagatatg caggtattcg agtgcctaga aataaagcaa ttgttggcca
     6001 aaatgcattt agtcatgaat caggtattca ccaagatggc gtattaaaac atcgtgaaac
     6061 atatgaaatt atgacacctc aacttgttgg tgtaagcacg actgaacttc cattaggaaa
     6121 attatctggt aaacacgcct tctcagagaa gttaaaagca ttaggttatg acattgataa
     6181 agaagcgcaa atagatttat ttaaacaatt caaggccatt gcggacaaaa agaaatctgt
     6241 ttcagataga gatattcatg cgattattca aggttctgag catgagcatc aagcacttta
     6301 taaattggaa acactgcaac tacaatatgt ctctagcggc cttcaaagtg ctgttgttgt
     6361 tgttaaagat aaagagggtc atatttacca ggattcaagt attggtactg gttcaatcgt
     6421 agcaatttac aatgcagttg atcgtatttt ccagaaagaa acagaattaa ttgattatcg
     6481 tattaattct gtcactgaag gtactgatgc ccaagcagaa gtacatgtaa atttattgat
     6541 tgaaggtaag actgtcaatg gctttggtat tgatcatgat attttacaag cctcttgtaa
     6601 agcatacgta gaagcacatg ctaaatttgc agctgaaaat gttgagaagg taggtaatta
     6661 attatgactt ataacattgt tgccctacct ggtgatggaa tcggtccaga aattttgaac
     6721 ggatctctat cattgcttga aattataagt aataaatata actttaatta tcaaatagag
     6781 caccacgaat ttggtggtgc ctctattgat acattcggcg agcctttaac tgagaaaacc
     6841 ttaaatgcgt gtaaaagagc agatgctatt ttactgggtg caatcggtgg acctaaatgg
     6901 acagatccta acaatcgacc agaacaagga ttattaaaat tgcgtaaatc cttaaattta
     6961 tttgtaaata tacgccccac taccgttgtc aaaggcgcta gttctttatc acctttaaag
     7021 gaagaacgcg ttgaaggcac agatttagtt atagtccgtg aattgacaag tggtatttat
     7081 tttggagaac ctagacattt taataatcac gaggccttag attctcttac ttatacaaga
     7141 gaagaaatag aacgcattgt tcacgtagca tttaaattgg ccgcttcaag acgaggaaaa
     7201 ctaacatcag ttgataaaga aaatgtatta gcttctagta aattgtggcg caaagtcgta
     7261 aatgaagtaa gtcaattata tccagaagta acagtaaatc acttacttgt tgatgcttgt
     7321 agtatgcatt taatcacaaa tccaaaacaa tttgacgtca tcgtatgtga aaacttattt
     7381 ggcgatattt taagtgatga agcttcagtg attcctggtt cacttggttt atcaccttct
     7441 gctagtttta gtaacgatgg tccaagattg tatgagccta ttcatggatc agcaccagat
     7501 attgcaggta aaaacgttgc caatccattt ggaatgattc tatctttagc gatgtgttta
     7561 cgtgaaagct taaatcaacc agatgctgca gatgaattag aacaacatat ttatagcatg
     7621 attgaacatg ggcaaacgac agcagattta ggcggcaaat tgaatactac tgatattttc
     7681 gaaattctat ctcaaaaatt gaatcactaa gggggagatg taaatgggtc aaacattatt
     7741 tgacaaggtg tggaacagac atgtgttata cgggaaattg ggcgaaccgc aactattata
     7801 cattgattta caccttatac atgaagttac ttctcctcaa gcatttgaag gacttaggct
     7861 tcaaaacaga aaattaagac gcccagattt aacatttgca acactcgatc acaatgttcc
     7921 tactattgat atattcaata ttaaagatga aattgcaaat aaacaaatca caacattaca
     7981 aaaaaacgcc atagattttg gggtgcatat ttttgatatg ggttctgatg aacaaggtat
     8041 tgttcacatg gtaggacctg agacaggact tacacagcct ggcaagacaa tcgtttgtgg
     8101 tgactctcac acagcaacac atggagcatt tggtgctatt gcatttggaa ttggaacaag
     8161 tgaagttgaa catgttttcg caactcaaac gctatggcaa acaaaaccca aaaacttaaa
     8221 aatcgatatt aatggtacct taccaacagg cgtctatgct aaggacatta ttctgcattt
     8281 aattaaaacg tatggtgttg actttggtac aggctatgct ttggaattta ctggcgaaac
     8341 aattaaaaac ctttcaatgg acggtcgaat gactatttgt aacatggcta tcgaaggtgg
     8401 tgccaaatac ggcataatcc aacctgatga tataacattt gaatatgtta aagggagacc
     8461 atttgccgat aacttcgcta aatcagttga taagtggcgt gagctatatt ctgatgacga
     8521 cgcgatattt gatcgtgtaa ttgaacttga tgtttcaaca ttagaaccac aagtgacatg
     8581 gggaactaat cctgaaatgg gtgttaattt cagtgaacca ttccctgaaa tcaatgatat
     8641 caacgatcaa cgtgcgtatg attatatggg gttagaacca ggtcaaaaag ctgaagacat
     8701 cgacttaggg tatgtttttc tcggttcatg tacaaatgct agactatcag atttgattga
     8761 agctagtcat attgttaaag gaaataaagt tcatccaaat attacagcta ttgtcgtacc
     8821 aggttctcgt acagtaaaaa aagaagcaga aaaattaggt ctagatacta tctttaaaaa
     8881 tgcaggattt gaatggcgtg aaccaggatg ttcaatgtgt ttaggcatga atcctgacca
     8941 agtacctgag ggcgtacatt gtgcatctac aagtaatcga aactttgaag gacgacaagg
     9001 caaaggtgca agaacacatt tagtatcccc tgctatggca gcagcagcag ctattcatgg
     9061 taaatttgtg gacgtaagaa aggtggttat ttaaatggca gcaatcaaac ctattacaac
     9121 atataaaggt aaaatagtcc ctctcttcaa cgacaatatc gatacagacc aaatcattcc
     9181 taaggtacac ttaaagcgta tttcaaaaag tggctttggt ccatttgctt ttgatgaatg
     9241 gcggtactta cctgatggtt cagataatcc tgatttcaat cctaacaaac cacaatataa
     9301 aggggcttct attttaatta ctggagataa ttttggatgt ggtttaagtc gtgaacatgc
     9361 tgcttgggct cttaaggact atggttttca tattattatt gcaggaagtt tcagtgacat
     9421 attttatatg aattgcacta aaaatgcgat gttgcctatc gttttagaaa aaagtgcccg
     9481 tgaacatctt gcacaatatg ttgaaattga ggtcgattta ccaaatcaaa ctgtgtcatc
     9541 accagacaag cgtttccatt ttgaaattga tgaaacttgg aagaataaac ttgtaaatgg
     9601 cttagatgac attgcaatca ccctacaata tgaatcatta atagaaaaat atgaaaaatc
     9661 actttaaggg agttgaatat tatgacagtc aaaacaacag tttctacgaa agatatcgat
     9721 gaggcatttt taagacttaa agatattgtc aaagaaacac ctttacaatt agaccattac
     9781 ttatctcaaa agtatgattg taaagtctat ttaaaacgag aagatttaca atgggtacgt
     9841 tcttttaaat taagaggtgc ttacaacgct atttctgttt tatcagatga agctaaaagt
     9901 aaaggtatta catgtgcgag tgcaggtaat catgctcaag gtgttgccta tacagctaaa
     9961 aaacttaatt taaacgctgt tatctttatg ccagtcacta cacctttaca aaaggtaaat
    10021 caagtaaagt tctttggaaa tagtaacgtt gaagttgtac tcactggtga tacatttgat
    10081 cactgtttag ctgaagcttt aacttataca agtgaacatc aaatgaactt tatagatcca
    10141 ttcaataatg ttcatacaat ttctggacaa ggtacgcttg ctaaagaaat gctagaacaa
    10201 tcaaagtctt acaatgttaa ctttgattat ctatttgccg caattggtgg tggcggttta
    10261 atttcaggta ttagtactta ctttaaaacc tattcaccta ccacgaaaat tataggtgtt
    10321 gaaccttcag gtgcaagtag tatgtatgaa tctgttgtcg taaataatca ggtagtcaca
    10381 ttgcctaata tcgataaatt tgtggacggt gcatctgtag ctagagttgg cgatattaca
    10441 tttgaaattg caaaagaaaa tgtagatgat tacgttcaag tagatgaagg tgcagtttgt
    10501 tctacgattt tagatatgta ttcaaaacaa gcaattgtag cagaacctgc tggcgcatta
    10561 agtgtgagtg ctcttgaaaa ctacaaagac cagattaagg gtaaaactgt tgtttgtgtc
    10621 attagtggtg gtaataatga tattaatcgt atgaaggaaa ttgaagaacg ttctttatta
    10681 tatgaagaaa tgaaacatta ttttatttta aatttccctc aacgccctgg tgcattaaga
    10741 gaatttgtaa atgatgtttt aggcccacaa gacgatatta caaaatttga atacttgaaa
    10801 aaatcttctc aaaatacagg tactgtcatt attggtattc aacttaaaga tcatgatgat
    10861 ttaatacaac tcaaacaacg tgtaaatcat ttcgatcctt ccaatattta tattaatgaa
    10921 aataagatgt tatattcatt gttaatttaa cacatattaa gaaaagcagt cataaattga
    10981 tttctaattg aaatcatctt atgactgctt tttattatgc tttacatttc tcgtttcgtc
    11041 agattcaaac gttttcactt cgccaagcca tttttctttg tctttacttt tttactttgc
    11101 tactttagac ataaaaaaag agaccttacg gtctca
//
LOCUS       DAHHYJ010000089        11131 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000089 DAHHYJ010000000
VERSION     DAHHYJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11131
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.089"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            115..1023
                     /locus_tag="NBK22_001779"
     CDS             115..1023
                     /locus_tag="NBK22_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD2414963.1"
                     /translation="MFKRTKLILIATILLSGCSAMESESKKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHRKEFEKVGEQFFMDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNADDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEEKYQYTGFTNEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDK
                     EFKEGMQRARKELNYTANTDVVSTLFSTKKNFTKDNTVDDVIELSNKLKDKPNMPQKS
                     QVTIQLGKSSINTKKPFYDDINPIEG"
     gene            1478..2404
                     /locus_tag="NBK22_001780"
     CDS             1478..2404
                     /locus_tag="NBK22_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD2414964.1"
                     /translation="MLKKAKLILIATLLLSGCSMMKNESSKDTNTETKSVPEEMDASK
                     YVGQGFQPPAEKDAIEFSKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVH
                     CDDHDIVFNASIPFDKSIIDSNSSLISKDKGDDMSTLVGTVLSGFEYRAHKEELDNLT
                     EVLKEYKSKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKKYFQPLIKEDDK
                     SFRDGMRNSKKQLKDKSRPYVVTTLFSTKDNFTKDNTIDEMIDFSEVLKKKKNIPHDL
                     NVSLQISNKYINTTRPNYSKKDVIEVGVFNHE"
     regulatory      2663..2797
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2859..3404
                     /locus_tag="NBK22_001781"
     CDS             2859..3404
                     /locus_tag="NBK22_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD2414965.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPIAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(3510..3758)
                     /locus_tag="NBK22_001782"
     CDS             complement(3510..3758)
                     /locus_tag="NBK22_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCD2414966.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            3866..4819
                     /locus_tag="NBK22_001783"
     CDS             3866..4819
                     /locus_tag="NBK22_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD2414967.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLEKINEFNAEMEIMLHPRYTFESMGQTFQAIQLLVQTMSNTM
                     QQDFRFVPISQNNKIQETVKIVYNYIKENQLQIEFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTRLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVEQEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            4809..6188
                     /locus_tag="NBK22_001784"
     CDS             4809..6188
                     /locus_tag="NBK22_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCD2414968.1"
                     /translation="MLHDILRNKFGFESFKLGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PLLQQSGPTIVYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVKNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            6341..7801
                     /gene="ebpS"
                     /locus_tag="NBK22_001785"
     CDS             6341..7801
                     /gene="ebpS"
                     /locus_tag="NBK22_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCD2414969.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHETIKEAENNTEHSTVSDKSEA
                     EQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGA
                     AGAMGVSKAKKHSNDAQNKSNSDKSNNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLA
                     GGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGAL
                     AIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQD
                     KATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRI
                     AIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            7926..8042
                     /locus_tag="NBK22_001786"
     CDS             7926..8042
                     /locus_tag="NBK22_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414970.1"
                     /translation="MLFNKVLSRILAYQLNFLNSHQKDKTPQSTNLTRENKK"
     gene            8203..9189
                     /locus_tag="NBK22_001787"
     CDS             8203..9189
                     /locus_tag="NBK22_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCD2414971.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQNMLAKKQTPLES"
     gene            complement(9304..10272)
                     /locus_tag="NBK22_001788"
     CDS             complement(9304..10272)
                     /locus_tag="NBK22_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCD2414972.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVTNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNDKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            10349..11008
                     /locus_tag="NBK22_001789"
     CDS             10349..11008
                     /locus_tag="NBK22_001789"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCD2414973.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQI
                     K"
BASE COUNT         4245 a         1668 c         1922 g         3296 t
ORIGIN      
        1 agccttttat gatgataatc gtccaataga atatggggtg cacagtaaag atgaataaaa
       61 ttttaaaaaa tttcttttaa tattctaaac aaacacataa atgggtgatg agctatgttt
      121 aaaagaacta aactaatctt aatagcaaca atactgctat caggatgttc agcgatggaa
      181 agtgaatcaa aaaaagacac gaatacagaa acaaaatctg tgccagaaga aatggaagct
      241 tcaaaatatg taggtcaagg tttccaacca cctgcagaaa aagatgcgat tgaatttgcg
      301 aagaagcatc gtaaagaatt tgaaaaagta ggtgaacaat tttttatgga caacttcggt
      361 ctaaaagtta aagctacaaa tgttgtaggt aaagatgatg gtgtagaagt ttatgtgcat
      421 tgcgaagatc atggcattgt atttaatgca agtttacctt tgtacaaaga tgccatccat
      481 caaaaaggat caatgcgtag taatgacaac gctgatgata tgagtatgat ggtaggtaca
      541 gtactcagtg gctttgaata tcgagcgcaa aaagaaaagt atgataactt atataaattc
      601 tttaaagaaa atgaagagaa atatcaatat acaggattta caaacgaggc aattaacaag
      661 acacaaaacg tcgggtatca aaatgaatac ttttatatta catatttatc aagaaactta
      721 aaagaatatc gtaaatatta tgaaccattg attcataaaa atgataaaga atttaaagaa
      781 ggtatgcaac gagccagaaa agagctaaat tatactgcaa atactgatgt agtttctacg
      841 cttttttcta ctaagaaaaa ctttactaaa gataatacag tagatgatgt gattgaattg
      901 agtaataaat taaaagataa acccaatatg cctcaaaaat ctcaagttac tattcaatta
      961 ggaaaatcta gtataaatac taaaaaacca ttttatgacg atataaatcc aattgaaggg
     1021 taagtgaata taaagtgaat aaaataaatg atagggacat agcagaattg aacggttatt
     1081 gggtttatca agatataaat gaatgataga ttttaaagta aatgaaaaac ggttcaaaca
     1141 aatagaaaat cataatatga aaggcatacc ttaatataga aatatacgag tttcttgatg
     1201 atcaggggaa tctaacttgt caacagataa taatctatca aggaacttca aatgaggcag
     1261 taaatcctaa taacccattg aaatctttag gtaatggaga tgattggctt caaaatgcta
     1321 aatcaacgga taattaaata aaaatcagac attgtttaac gcaagtagat gtatttgaag
     1381 atttatatat agagaaacta taagacgtaa ataaattaag agagtataat tatttgcaaa
     1441 atgaaactta taaaagcgca taaatgggtg atgagatatg cttaaaaaag caaaattaat
     1501 cttaatagca acacttctac tatcaggatg ttccatgatg aaaaatgaat caagtaaaga
     1561 cacgaataca gaaactaaat ctgtaccaga agaaatggat gcttcaaaat atgtaggaca
     1621 aggattccaa ccacctgcag aaaaagatgc gattgaattt tcgaagaagc ataaagataa
     1681 aattgctaag cgaggcgaac aattttttat ggataacttc ggtctaaaag ttaaagctac
     1741 aaatgttata ggtagtggcg acggtgtaga agtattcgtg cattgtgatg accacgacat
     1801 cgtatttaat gcaagtattc catttgataa atcaattatt gacagtaata gttcattaat
     1861 aagtaaggat aaaggcgatg atatgagtac tttagttggt acagtgttga gtggctttga
     1921 atatcgagca cataaggaag agttggataa tttaacagaa gttttaaaag aatataaaag
     1981 taaatataaa tatactggtt atactgaaaa tgcaattatg aaaacgcaga atagtggttt
     2041 tagaaatgag tattattatt tgactgcaat tccatatact ttagatgaat ataaaaagta
     2101 ttttcaaccc ttaatcaaag aggatgataa gtcttttagg gacggtatga gaaactcaaa
     2161 aaaacaactt aaagataaat cacgtccata tgttgttaca actttattta gtactaaaga
     2221 taattttact aaagataata caatagacga gatgattgat ttttctgaag tgctaaaaaa
     2281 gaaaaagaat attcctcatg atttgaatgt ttctcttcaa atttctaata aatatataaa
     2341 tacaacaaga cctaattata gtaagaagga tgtaatagaa gtaggtgtat ttaatcatga
     2401 ataagctaac acgaatgatt aaactaaaaa caataaaaag ataggttcaa aaaagccatc
     2461 ctaacaacag acaaaaccac aaaccaatca aacctaccca accaaagtac gcaaatcact
     2521 aatcaaacct actcaaccaa tgaacgcaaa tcaccaatga aacactctcc aattacgtaa
     2581 ccatgatcta ttttaagcat agttattgag gttttgtgat atatagtata aaattaatga
     2641 gaattaaatt taataatgta aaattcatct tcggggtcgg gtgtaattcc caaccggcag
     2701 taaattaagc ctgcgacctg ctagtatgtt tcatattagt ggctgatcta gtgagattct
     2761 agagccgaca gtatagtctg gatgggagaa gatggaggtt ttttgttgtg caataatcct
     2821 cctattctta cgagatgaat ggaaggagaa aattgaatat gcaacaaaat aaacgtctta
     2881 tcacaataag tatgttgagc gcgattgcgt ttgtgttaac ttttatcaag tttcctatac
     2941 catttttgcc accatactta actttagatt ttagtgatgt accgtcattg ctagctacat
     3001 ttacgtttgg accaattgcc ggtatcgtag ttgcactggt taaaaacttg ctgaactact
     3061 tatttagtat gggcgatcca gttgggccgt ttgctaactt tttagcaggc gcaagtttct
     3121 tattaactgc ttacgccatc tataaaaata aacgttcaac aaaatctttg attactggat
     3181 taatcattgc aacaatcgtt atgactatcg tgttgagtat tttgaactat ttcgttctat
     3241 tacctttgta cggtatgata tttaacttag ctgatatcgc aaataatctt aaagtaatca
     3301 ttgtttcagg aattatacca ttcaatatta ttaaaggtat cgttatttct attgtattta
     3361 ttttactata tagaaggctt gcgaatttct tgaaaagaat ttaattaaat taaagcaaaa
     3421 taatatacac ataataataa aaagcaggtg actatcaata aacgatagct tgcctgcttt
     3481 ttctatggaa cctttatcta aaaaattaat tattcaaatt ttaaagcgtc cccatcaaat
     3541 gattcgtctg caattttaat agaatctgta gggcatccat caattgcatc ttccatatct
     3601 tcatataatt cctcaggtac ttctgcagta ccttggttat cgtcaaggat tacgaaagca
     3661 ataccttcgt cgtcgtaatc atatatatct ggcgctgctg caccgcatgc accacatgca
     3721 atacaagtat ccatatcaac gattgtatat tttgccaatg tcttcgcctc ctttgataaa
     3781 aatgctaaaa tagtaatgtg actaaaattt tagacagcat catttttatt ttcaaattat
     3841 ccgttttaca gagtgagggt taaacttgca acacattata aaaacagcat tacaacaaac
     3901 atttaactat aaaacaaata aaagtattta caatatctta gttggtaaga aatctcacca
     3961 aacctttttt gacgcttgta gtcaacaaca gttgtcatta tatcacagtt taccactatt
     4021 aaaatatccg tcttttgagc tatttttaga aaaaatcaat gaatttaatg ctgaaatgga
     4081 aatcatgttg catcctagat atacatttga aagcatgggg caaacatttc aagcaattca
     4141 actattagtg caaaccatgt ctaatactat gcagcaagat tttcgtttcg taccaatctc
     4201 tcaaaataat aagatacaag aaactgtaaa gattgtctat aactacatta aagaaaacca
     4261 attacaaatt gaatttgaaa atgaactaca taacttgttt aaagcaataa ctttaaaagg
     4321 gccatgttac ttacattatt atttgcaagg ctatgatgaa ccaatgtata cgagacagca
     4381 agttagttta atagaaaaac tatctcaaca gcaattgttt gaatacgaaa tgaataattt
     4441 agtgacaatg atgtttgaat tagaaagtgg agaatatact attttatcaa agataataat
     4501 gaaacctaca ttattaaatc aaacctatat tacttataca aggttgcttg aacaattcac
     4561 gatggaagat atagcggctc aacaacaagt taaaatcaat actatcgaag atcatgtact
     4621 tgaaatctta atcaaaggtt atatgtctaa ttacgatgat tatgttgaac aagaagatca
     4681 actacaattt ttaaattttt atcaacaaca tcgtggtgag cgattaaaat tttacaaaga
     4741 acaatttgac acgttatcat attttcaatt aaaagtatta atcgttggat ttgaaagagg
     4801 tgatctgaat gttgcatgat attttacgaa acaaatttgg cttcgagagt tttaaactgg
     4861 gacaacagga aattatagaa agtataatgt ctcaacagca cactctaggt attcttccga
     4921 ctggaagtgg aaagagtttg tgttatcaaa tacctacgta tttatcaggt aagccgacat
     4981 taattatctc accgttaata tctttaatgg atgaccaagt tatgcagttg aaaataaatg
     5041 gagaaaaacg tgtaacatgt attcactctg gtatggatga aattgagaaa aagcataata
     5101 ttaaatgttt acgacatagc cgtttcatct ttctaagtcc agaatttctc ttgcaaccgt
     5161 caaattttaa attaatatct atgatagact ttggcatgat tgttcttgat gaagcacatt
     5221 gcctatctga atggggatat gatttcagac cacattatgc tctaatagga aaagtaacaa
     5281 agcattttaa agaagcggtt gtcttagcat tgacagcaac tgcaccaccg catttacaag
     5341 atgatttgac ggaaatgtta gcgattcaat tcaatgttat taaaactaca atgaatcgcc
     5401 caaacataag ctttaagcat cttaattttc atgatgatga agataaaatt gaatggttgc
     5461 tgccgcttct acaacagtcg ggaccaacga ttgtttatgt ctcatcgaaa aagatgtgtc
     5521 tgaatttagc gcaacttatt tatgattcag gttttcttac aggcatatat catggtgata
     5581 tgaattatca agagcgacac acagttcaac aacaattttt aaataatgat attccgatta
     5641 tagtcgcaac gagtgctttt ggtatgggaa ttaataaaaa agatattcgc acaatcattc
     5701 actttcatct ttcaacaagt ccttctaact acattcaaga aattggccgt gcgggtcgcg
     5761 atggtgaact aagtcaggca attagtttat tccaaccgga cgataaatat attttagaaa
     5821 cgttattatt tgcagatatg ataacagaag aagatgtaaa aaatttcgaa ataggagaat
     5881 ttttagctcc cgataaacaa gacgttttga caacgttgca ttcattctat agtatcggcg
     5941 ccttgaaaca gatatttaag caatcattta aacgaaagca attaggattc tttcgcatga
     6001 ttggctattg caaattggat caatgtagac ggaagtattt attagaattt ttcggtgaat
     6061 atccaccggc acaagatcga tgttgtgaca atgattctaa tataactgat atcgcaattt
     6121 taaataagaa gaaggtaatt agaagtattg gatttgatga aaagttgcaa aatttatttc
     6181 tcagatagta ttactttact aaaagaaaat tgacaagcta taattagtgt atacacaatt
     6241 gaaaaatgat tgaaataatt ttgaaaaata tacataaaca tatgtcatgt gggtatattt
     6301 tatgtaaaat cattgtaata gaatagaaag gaagatggct atgtctaata attttaaaga
     6361 tgactttgaa aaaaatcgtc aatcgataga cacaaattca catcaagacc atacggaaga
     6421 tgttgaaaaa gaccaatcag aattagaaca tcaggataca atagagaata cggagcaaca
     6481 gtttccgcca agaaatgccc aaagaagaaa aagacgccgt gatttagcaa cgaatcataa
     6541 taaacaagtt cacaatgaat cacaaacatc tgaagacaat gttcaaaatg aggctggcac
     6601 aatagatgat cgtcaagtcg aatcatcaca cagtactgaa agtcaagaac ctagccatca
     6661 agacagtaca cctcaacatg aagaggaata ttataataag aatgcttttg caatggataa
     6721 atcacatcca gaaccaatcg aagacaatga taaacacgag actattaaag aagcagaaaa
     6781 taacactgag cattcaacag tttctgataa gagtgaagct gaacaatctc agcaacctaa
     6841 accatatttt gcaacaggtg ctaaccaagc aaatacatca aaagataaac atgatgatgt
     6901 aactgttaag caagacaaag atgaatctaa agatcatcat agtggtaaaa aaggcgcagc
     6961 aattggtgct ggaacagcgg gtgttgcagg tgcagctggt gcaatgggtg tttctaaagc
     7021 taagaaacat tcaaatgacg ctcaaaacaa aagtaattct gacaagtcga ataactcgac
     7081 tgaggataaa gcgtctcaag ataagtctaa agatcatcat aatggcaaaa aaggtgcagc
     7141 gatcggtgct ggaacagcag gtttggctgg aggcgcagca agtaaaagtg cttctgccgc
     7201 ttcaaaacca catgcctcta ataatgcaag ccaaaaccat gatgaacatg acaatcatga
     7261 cagagataaa gaacgtaaaa aaggtggcat ggccaaagta ttgttaccat taattgcagc
     7321 tgtactaatt atcggtgcat tagcgatatt tggaggcatg gcattaaaca atcataataa
     7381 tggtacaaaa gaaaataaaa tcgcgaatac aaataaaaat aatgctgatg aaagtaaaga
     7441 caaagacaca tctaaagacg cttctaaaga taaatcaaaa tctacagaca gtgataaatc
     7501 aaaagaggat caagacaaag cgactaaaga tgaatctgat aatgatcaaa acaacgctaa
     7561 tcaagcgaac aatcaagcac aaaataatca aaatcaacaa caagctaatc aaaatcaaca
     7621 acagcaacaa caacgtcaag gtggtggcca aagacataca gtgaatggtc aagaaaactt
     7681 ataccgtatc gcaattcaat actacggttc aggttcaccg gaaaatgttg aaaaaattag
     7741 acgtgccaat ggtttaagtg gtaacaatat tagaaacggt caacaaatcg ttattccata
     7801 atataactat ataaattgta actgaactgc tatatacaaa cgtgcatatt ataagtatct
     7861 ctaaatattt tggagaactt aaattgcgct tataagtatg tagcggtttt ttcatttttc
     7921 aaagtttgtt atttaacaag gtcttgtctc gaatattggc atatcaatta aactttttaa
     7981 atagtcatca aaaagataaa acaccacaat caacaaattt aacgagggag aataaaaaat
     8041 aaatcaacat attaaattgt agtgttattc aactccgtag ctaacaattc tctatgcaca
     8101 ttaaacaaat tgtcaaaaat atatcataaa tcttcaagca cagacttagc gcatcaatca
     8161 ctgaactgtt ataatagttt gggattaaag gaggccgaaa caatgcaaaa agttgaaagt
     8221 atcataattg gtggagggcc atgcggatta agtgcggcta ttgaacaaaa aagaaaaggt
     8281 attgatacct taattattga aaaggggaat gtcgttgaat caatctataa ttatcctact
     8341 caccaaacat ttttctcatc aagtgataaa ttaagtattg gggacgtacc gtttatcgtt
     8401 gaagaaagta aaccaagacg taatcaagcg ctagtttatt accgagaagt tgtaaaacat
     8461 catcaattaa aagtaaatgc atttgaagaa gtattaactg ttaaaaaaat gaataataaa
     8521 tttactatta ctacgacgaa agatgtttat gaatgtcgat ttttaacaat cgcgacaggc
     8581 tattatggtc agcataatac attagaagtt gaaggagcgg atttacctaa agtgttccat
     8641 tattttaaag aggcacatcc gtattttgat caagatgttg ttattattgg tggtaagaat
     8701 tcggctatcg atgctgcttt ggagttggaa aaagctggtg ctaacgtgac ggttctatat
     8761 cgtggtggag attattcgcc ttcaattaaa ccgtggatac ttccaaattt cacagcatta
     8821 gtaaatcatg aaaaaattga catggaattt aatgctaatg ttacccaaat aactgaagat
     8881 actgtgactt atgaagtaaa tggtgaaagt aaaacaatac acaatgatta tgtatttgcg
     8941 atgattggtt atcatcccga ttatgaattt ttaaaatctg taggcattca aattaataca
     9001 aatgaatttg gaacagcgcc tatgtataat aaagaaacat acgaaacaaa tatcgaaaat
     9061 tgctatattg caggtgtaat tgctgcaggg aacgatgcga ataccatttt tattgaaaat
     9121 ggtaaattcc acggtggcat tattgctcaa aacatgctag ctaagaaaca aacgccgtta
     9181 gaatcataaa aataaaggtc tatgtactaa ataacttagt tttacaacga ctgacattca
     9241 tgatatgtca aatgaggttg atgactaatt attgtacata gaccttttta tgttacgtat
     9301 tcattataat tcaaaatatg atttgatttc agctttatct aaattgttgc ttaacgcaac
     9361 taataatttt aatcttgctt ttggaccatt caaaccgtta gaaaaaataa aaccttgttg
     9421 tgcgagttgg taaccaccac catcgtatgc gtaagttgga ctcacaatac cattaaagga
     9481 acgtgaaact agcacaatag gtatatttaa agatactaat tgttgaatgc cttctaatgc
     9541 gcttggaggt atgttgcctt gtcctaacgc ttcaataacc ataccatcga taccttcacg
     9601 actataaaaa ctaaaaatgt cacctggcat acccatatat gcttttacta atggtacatt
     9661 tagtttgtca ttgacatttt ccaatgcttg ttggcgatat ggcatatgat ggaattgcac
     9721 acgatccttt gtcaatacac ctagcggacc atgatttgga ctttgaaatg tgtttgtatt
     9781 agacgtatgt gttttggtaa cattacgcgc cgtatgaatt tcatcattaa atacaaccat
     9841 cacgccttta tgacgggcct tttcatcaga ggcaacgcga atagcggaaa tataattata
     9901 taatccgtca gaaccaattt cattagacga gcgcattgcg ccagtaataa caacaggttg
     9961 ctcgatacct aatattaaat caagtaaaaa ggcagtttct tctaacgtat cggtaccatg
    10021 cgtgataacg aaaccatcat aatatttatt tgttactgct tctaaaataa tatcctttaa
    10081 ttgtttaaca tgttggattg tcatatgagg tgatggtaca ttaaaaggat ttaattcatc
    10141 tatttgtgca tattgattta tgacatcttg atgcattgaa atagggttga tatcatttgt
    10201 tactacttta ttagattggt cttgtgacat actaatggtg ccaccagtat gaataacaag
    10261 tagatgtttc atatatttcc tcctatattt aatttaccta attatgataa aatattattc
    10321 ataaaacgac aaggaaggga aatgacgcat gaaagccatt aatattgcat tagatggtcc
    10381 agctgctgcc ggaaaaagta caattgcgaa acgtgtagcc agcgaactat caatgattta
    10441 tgtcgataca ggagcaatgt atcgtgcatt aacatacaaa tatttaaaat taaacaaaac
    10501 tgaggacttt gcaaaactag ttgaccaaac aacattagat ttaacttata aagcagataa
    10561 aggtcaatgt gtcattttag ataacgaaga tgtaacagac tttttaagaa ataatgatgt
    10621 gacgcaacat gtttcatacg ttgcatctaa agagccagta cgttcattcg ccgttaaaaa
    10681 acaaaaagag ttagctgcag aaaaaggtat cgtaatggat ggtcgcgata tcggaactgt
    10741 agtgctacca gatgcagatt taaaagtata tatgattgca tcagttgaag agcgagcaga
    10801 aagaagatat aaagataatc aattaagagg tatcgaatca aattttgaag atttaaaacg
    10861 tgatattgaa gctcgtgatc aatatgacat gaaccgtgaa atatcaccat taagaaaagc
    10921 agatgatgca gtgacattag atacgacagg caagtcgatt gaagaagtta ctgacgaaat
    10981 tttagcgatg gtgagtcaaa ttaaataatt aagaagctaa cattaataaa atttataatt
    11041 acttgatgaa cggaaaaggc tgtttttatt ctaatcgaat agaaatggtc ttttgttaat
    11101 ttataaacga ataacgataa tactagaaat a
//
LOCUS       DAHHYJ010000090        10748 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000090 DAHHYJ010000000
VERSION     DAHHYJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.090"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            9..2606
                     /gene="clfB"
                     /locus_tag="NBK22_001790"
     CDS             9..2606
                     /gene="clfB"
                     /locus_tag="NBK22_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCD2414974.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTTKPMST
                     QTSNTTTTEPASTNETPQPTAIKNQATAAKMQDQTVPQEANSQVDNKTTNDANSIATN
                     SELKNPQTLDLPQSSPQTISNAQRTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNGQ
                     VTASDFKLEKTTFDPNQSGNTFMAVNFKVAGKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVAKATYDILTKTYTFVFTDYVNNKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSAKDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTNEFKDKITYKYQNVASINFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     IDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPKDPTPGPPVDPEPSPDPEPKPTP
                     DPEPSPDPDPDPDSDSDSDSGSDSDSGSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSNSDSDSDSESDSDSESDSDSESDSDSDSDSDSESDSDSDSDSESDSDSDS
                     DSDSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSESDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPN
                     NEQKAPSNPKGEVNHSNKVSKQHKTDALPETGDKSENTNATLFGAMMALLGSLLLFRK
                     RKQDHKEKA"
     gene            2722..3483
                     /locus_tag="NBK22_001791"
     CDS             2722..3483
                     /locus_tag="NBK22_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD2414975.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDTTAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIIGNLSSVKETEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            3728..4183
                     /locus_tag="NBK22_001792"
     CDS             3728..4183
                     /locus_tag="NBK22_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD2414976.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES"
     gene            complement(4286..4396)
                     /locus_tag="NBK22_001793"
     CDS             complement(4286..4396)
                     /locus_tag="NBK22_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414977.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(4466..5890)
                     /locus_tag="NBK22_001794"
     CDS             complement(4466..5890)
                     /locus_tag="NBK22_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCD2414978.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDSHTKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKNDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKRF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLKRLLKKEKGEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6088..6288
                     /locus_tag="NBK22_001795"
     CDS             6088..6288
                     /locus_tag="NBK22_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414979.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DTESSNQEPSLQQAKEQKDNKG"
     gene            6314..6979
                     /gene="nsaR"
                     /locus_tag="NBK22_001796"
     CDS             6314..6979
                     /gene="nsaR"
                     /locus_tag="NBK22_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCD2414980.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSLTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            6990..7877
                     /gene="nsaS"
                     /locus_tag="NBK22_001797"
     CDS             6990..7877
                     /gene="nsaS"
                     /locus_tag="NBK22_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCD2414981.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLETALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            7985..8740
                     /locus_tag="NBK22_001798"
     CDS             7985..8740
                     /locus_tag="NBK22_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414982.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNKSKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLAVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKALMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            8737..10737
                     /locus_tag="NBK22_001799"
     CDS             8737..10737
                     /locus_tag="NBK22_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414983.1"
                     /translation="MKLRLLWHIVRRQFIMQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFVIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGLVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTITKLITNDYDLSFSDNSKSQ
                     IERQQTIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGAIFSRQSVMITAFTAQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKGFYAHEQKNSTISINFNVFGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNHHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQQRN"
BASE COUNT         3792 a         1676 c         1910 g         3370 t
ORIGIN      
        1 taatattttt gaaaaaaaga attgattatt tgtcgaataa gcagaataag tattcgatta
       61 gacgttttac agtaggtacc acatcagtaa tagtaggggc aactatacta tttgggatag
      121 gcaatcatca agcacaagct tcagaacaat cgaacgatac aacgcaatct tcgaaaaata
      181 atgcaagtgc agattccgaa aaaaacaata tgatagaaac acctcaatta aatacaactg
      241 ctaatgatac atctgatatt agtgcaaaca caaacagtgc gaatgtagat agcacaacaa
      301 aaccaatgtc tacacaaacg agcaatacca ctacaacaga gccagcttca acaaatgaaa
      361 cacctcaacc gacggcaatt aaaaatcaag caactgctgc aaaaatgcaa gatcaaactg
      421 ttcctcaaga agcaaattct caagtagata ataaaacaac gaatgatgct aatagcatag
      481 cgacaaacag tgagcttaaa aatcctcaaa cattagattt accacaatca tcaccacaaa
      541 cgatttccaa tgcgcaaaga actagtaaac caagtgttag aacgagagct gtacgtagtt
      601 tagctgttgc tgaaccggta gtaaatgctg ctgatgctaa aggtacaaat gtaaatggtc
      661 aagttacggc aagtgatttc aagttagaaa agactacatt tgaccctaac caaagtggta
      721 acacatttat ggcggtaaat tttaaagtgg cagggaaagt gaaatcaggg gattatttta
      781 cagcgaagtt accagatagt ttaactggta atggagacgt ggattactct aattcaaata
      841 atacgatgcc aattgcagat attaaaagta cgaatggcga tgttgtagct aaagcaacat
      901 atgatatctt gactaagacg tatacatttg tctttacaga ttatgtaaat aataaagaaa
      961 atattaacgg acaattttca ttacctttat ttacagaccg agcaaaggca cctaaatcag
     1021 gaacatatga tgcgaatatt aatattgcgg atgaaatgtt taataataaa attacttata
     1081 actatagttc gccaattgca ggaattgata aaccaaatgg cgcgaacatt tcttctcaaa
     1141 ttattggtgt agatacagct tcaggtcaaa acacatacaa gcaaacagta tttgttaacc
     1201 ctaagcaacg agttttaggt aatacgtggg tgtatattaa aggttaccaa gataaaatcg
     1261 aagaaagtag cggtaaagta agtgctaaag atacaaaact gagaattttt gaagtgaatg
     1321 atacatctaa attatcagat agctactatg cggacccaaa tgactctaac cttaaagaag
     1381 taacgaatga gtttaaggat aaaatcactt ataaatacca aaatgtagca agtattaatt
     1441 ttggcgatat tactaaaacg tatgttgtat tagtggaagg tcactatgat aatactggta
     1501 aaaacttgaa aacacaggtt attcaagaaa atattgaccc agcgacaggt aaagactaca
     1561 gtattttcgg ttggaataat gagaatgttg tacgttatgg tggtggaagt gctgatggtg
     1621 attcagcagt aaatccgaaa gacccaactc cagggccacc ggttgaccca gaaccaagtc
     1681 cagatccaga accgaagcca acgccagatc cagaaccaag tccagaccca gatccagatc
     1741 cggattcaga ttcagacagt gactcaggct cagacagcga ctcaggttca gatagcgatt
     1801 cggattcaga tagcgattca gactcagata gcgactcaga ttcggacagc gattcagatt
     1861 cggacagcga ttcagactca gatagcgact cagattcaga tagcaactcg gattcagaca
     1921 gtgactcaga atcagacagt gattcagaat cagacagcga ttcagaatca gacagtgact
     1981 cagattcaga tagcgattca gaatcagata gcgattcaga ttcagacagc gagtcagatt
     2041 cagatagcga ctcagattca gatagtgagt cagattcaga cagtgactcg gactcagata
     2101 gcgactcaga ctcagacagc gactcagact cagatagcga ttcagactca gacagcgact
     2161 cagattcaga tagcgattca gactcagaca gcgattcaga ctcagatagc gattcagact
     2221 cagacagtga ttcagaatca gacagcgact cagactcaga tagcgattca gattcagata
     2281 gcgactcgga ttcagatagc gattcggact cagatagtga ctcagactca gatagcgact
     2341 cagactcaga tagcgattcg gactcagata gcgactccga ttcaagagtt acaccaccaa
     2401 ataatgaaca gaaagcacca tcaaatccta aaggtgaagt aaaccattct aataaggtat
     2461 caaaacaaca caaaactgat gctttaccag aaacaggaga taagagcgaa aacacaaatg
     2521 caactttatt tggtgcaatg atggcattat taggatcatt actattgttt agaaaacgca
     2581 agcaagatca taaagaaaaa gcgtaaatac ttttttaggc cgaatacatt tgtattcggt
     2641 ttttttgttg aaaatgattt taaagtgaat tgattaagcg taaaatgttg ataaagtaga
     2701 attagaaagg ggtcatgacg tatggcttat atttcattaa actatcattc accaacaatt
     2761 ggtatgcatc aaaatttgac agtcatttta ccggaagatc aaagcttctt taataacgat
     2821 acaactgcta aaccattaaa aactttaatg ttgttacatg gattatcaag tgatgaaacg
     2881 acatatatga gatatacaag catagaaagg tatgcgaatg aacacaaatt agctgtgatt
     2941 atgcccaatg tagatcatag cgcatatgct aacatggcat atggtcatag ctattatgat
     3001 tatattttgg aagtatatga ttatgttcat caaatatttc cactttccaa aaagcgtgat
     3061 gacaatttta tagcaggtca ctctatggga ggatatggca caattaaatt tgcattaaca
     3121 caaggggata aatttgccaa agctgtacca ttatctgctg tgtttgaagc gcaaaattta
     3181 atggatctag agtggaatga tttttcaaaa gaggccataa ttggcaatct ttcaagtgtt
     3241 aaagaaactg aacatgatcc gtattacttg ctagacaaag ctgtagctga agataaacaa
     3301 attccaaaat tgctcattat gtgtggtaaa caagactttt tatatcaaga caacttagat
     3361 tttatcgatt atttatcacg caaaaatgtt ccttatcaat ttgaagatgg accaggagat
     3421 catgattatg catattggga tcaagcgatt aagcgtgcta taacatggat ggtgaatgat
     3481 taattatttc ttggaaaata tgtggctgca ttaaatacac agagtgagag atacaaacta
     3541 tttacgcacg actaacattt ctaagtgttt aaattatttt tgtattaata tgattggcgc
     3601 aatttgctga tacacaaaaa tgtttctcgt gaaacttaga tttagcttat agttttatca
     3661 tcatttgtat gacttacatt ataaatttta ttataatgag gttaacgctt tgaaaggagt
     3721 catcatcatg tcgaccaata aaaacgatta tgagcatatg ttgttttatt ttgcatataa
     3781 aacctttatt actaccgctg atgaaattat agagaagtat ggtatgagtc gtcagcatca
     3841 tcgttttttg ttttttatca ataaattacc tggtattact attaaatcat tactagaaat
     3901 attagaaatt tctaaacaag gatcacatgc aacacttcaa aaattaaaag agcaaggtct
     3961 cattattgaa aaagttttag agactgatcg acgtgtcaaa aaattatatt cgacggataa
     4021 aggcgatcaa ctcattgctg aattgaacaa ggcgcaagat gaattattgc aaaatatata
     4081 tcaacaagtc ggttcggatt ggtatgatgt gatggaagca ttggctaaag ggcgacctgg
     4141 ctttgatttt attaagcatt tgaaagatga aaaagaaagc tagcatcaga aatgttaaaa
     4201 atcttcgcat tcttaaattt aaaaaatatg tcaaaaagtg tataataaaa acatataatt
     4261 taattgaact cagtttcaac acatcttaga aaggagtttg aatgatgaaa aaattagcag
     4321 ttattttaac attagttggc ggtttatact tcgcatttaa aaaataccaa gaacgtgtta
     4381 accaagcacc taacattgag tactaaatta aaccataaaa aattcccgaa caccttgtta
     4441 tagtgctcgg gaattttttt atgctttact tgaatatatc aaatattatt tttgcgcttt
     4501 ctgtattttc gatattacca ctaaatgatt ctgatctagg tccataagcg taggtattaa
     4561 catcttcgcc tgtatgtcca tcggaagtcc accctgtata agatttatca tttactggct
     4621 tttgaatagc gtgttgtagg gcttttgttt gcgtttctac ttctgcggat ttttcgcctt
     4681 tttctttttt aagtagtctt tttagctttt tattctcttt tttaaccttt ttcatatcat
     4741 cttgtgaaaa ttcaaatcca taaccttcat taataacttt ttcagggtct tcacctttag
     4801 ccattttttc tgtcatatat gatccagagt gtttcataga tttaatcggt tgaggattcc
     4861 attcgtatcc tttatcttta ccaattgtta gaccaccagt agagtgatct gctgttgcaa
     4921 cgacaagtgt atctttatgt ttcttagcat attgaatggc gtcatcgaat gctttttcaa
     4981 aaccttccat ttcagacata acgcctgtaa tatcgttgga atgcgctgat ttatctatag
     5041 aagcaccttc gaccattaaa aagaatcttt ttttattacg ctcaagctta ctaagtgcac
     5101 tttgttgcat atcagctaat gatggttcat ctttagaagc atctattgca agtggcatat
     5161 ttttatctgc aaacaaacca agaactttat ctttatcaga ttttgataac tccttactgt
     5221 tcgtggcaag gtcgtaacca tcatttttga attttttatc taaattgcca ttacttttac
     5281 cgaaatattt agcgccgccg cctaataaaa catcaacttt atgctttccg ttgattttat
     5341 ctttataaaa ttgtttagcg atttcgtttt tatcatctct agaagtcacg tgtgcagcat
     5401 atgctgctgg tgttgcatct gttaattcag ctgttgaaac aagaccagtc gacttacctt
     5461 tttcttttgc acgttcaagc actgtcttta ctttctgctt gttactgtca acaccgatgg
     5521 caccattata tgtcttatgg ccagaactaa aggctgttcc gccagctgca gaatcagtaa
     5581 tattctgttt tgggtcattt gaatatgtac gatttgtgcc ttttaaatat gaatcaaaag
     5641 cagtaggggt catttctttg gtatgcgaat catttttata ataacgataa gctgtgttaa
     5701 atgatggacc catgccatcg ccaactaaaa agataacatt ttttggattt ttagtattac
     5761 caaccgcgaa actttcatct ttagaacttt tatcggattg cgcaattgca ggtgtgacag
     5821 aactaaaaac cgttgacacg ataataaggt tagcaactgc aaattttgtg gcttttttaa
     5881 ctgataacat aagacatcct cctgagtata tgactatgtc ttcagtgtaa aagaggaatt
     5941 ttgagcaatt atgtagtttt agttaaaaat atgtaaacag agtgatttag aataacaaaa
     6001 aatgaatata tatgacaatt tgttatagaa agcgttagaa tagaagcgtg tgaaaatata
     6061 gaattaaata taatttgagg tggaaaaatg atactagtaa tgttatctcc attattaatc
     6121 atattcttta tagtgttgtc tattttagag gagcgtaaac gtacgaagaa aaagcaactc
     6181 gagaaagaaa aagcaaatac actaaatcaa aatacaaatg acacggaaag ttcaaatcaa
     6241 gagccgtcat tgcagcaggc taaagaacaa aaagataaca aaggataatt caattgaagg
     6301 aagaagatta tagatgaaaa tattaattgt tgaagatgat tttgttatag cagaaagttt
     6361 agcatctgaa cttaaaaaat ggaattacgg tgttattgtc gttgaacaat ttgatgatat
     6421 actgtctatc tttaaccaac atcaacctca gcttgtattg ctagatatta atttgccaac
     6481 gttaaatggt tttcattggt gtcaagaaat ccgaaaaaca tctaatgtgc caattatatt
     6541 tattagttcc cgtattgata atatggacca aattatggca atacaaatgg ggggagatga
     6601 ttttatcgaa aaaccattta acttgtcatt aacgattgcc aaaattcaag cattattgag
     6661 acgaacttat gacttgtcag tagctaatga ttcattgacg gttaaaggtt gtacattaat
     6721 attagatgaa gcaaaagtcg tgtatcaaga acaaaacata cagctatctt tgactgaatt
     6781 acaaatatta aagttattat ttcaaaatga agataaatat gtaagtagaa ctgctttaat
     6841 tgaaaaatgt tgggaatcag aaaacttcat agatgataac acattagctg ttaacatgac
     6901 gcgcctgcgt aaaaaattaa atactattgg cgttaatgat tttatcatta caaagaaaaa
     6961 tgtcggatat aaagtatagg gtgaatgcaa tgacctttct taaaagtatt actcaggaaa
     7021 tagcaatagt catagttatt tttgctttat ttggcttaat gttttacctg tatcatttgc
     7081 cattagaagc atatttacta gcacttggcg ttattttatt attattactc atattcatag
     7141 gtattaaata tttaagtttt gtaaaaacta taagccaaca acaacaaatt gaaaacttag
     7201 aaactgcgtt gtatcagctt aaaaatgaac aaattgaata taaaaatgat gtagagagct
     7261 actttttaac atgggtacat caaatgaaaa cacccattac tgcagcacaa ctgttacttg
     7321 aaagagatga gcctaatgtt gtgaatcgtg ttcgtcaaga ggttattcaa attgataact
     7381 atacaagttt agcacttagt tatttaaagt tattaaatga aacttctgat atttctgtca
     7441 ctaaaatttc gattaataat atcattcgcc caattattat gaaatattca atacagttta
     7501 ttgatcaaaa aacaaaaatc cattatgaac cttgtcatca cgaagtatta actgacgtta
     7561 gatggacctc tttaatgata gaacaattaa taaataatgc acttaagtat gcgagaggta
     7621 aagatatatg gattgaattt gatgagcaat ccaatcaatt atacgtaaaa gataatggta
     7681 tcggtattag tgaagcggac ttgcctaaaa tatttgataa gggctattca ggttataatg
     7741 gccagcgcca aagtaactca agtgggattg gtttatttat cgtaaaacaa atttcaacac
     7801 acacaaacca tcctgtttca gtcgtatcta aacaaaatga gggtacaaca tttacgattc
     7861 aatttccaga tgaataaaaa ctttcaatat tgtaagtata ctagtaacat ttttttacta
     7921 atttaaatgt tattagtatt tttttgtttt aatatagaac taacaaagaa atgaggtgca
     7981 tgccatgttg ctagaagtga aacatgtaaa aaaggtttat ggtaaaggtt tgaatgctac
     8041 gacagcactt aatcaaatga atttatcagt tggagctggt gaatttgttg caattatggg
     8101 tgagtctggg tcagggaagt ctacactact aaatttaatt gcttcttttg atggactaac
     8161 tgaaggtgac attattgtgg atggcgcaca tttaaataat atgaaaaata aaagtaaagc
     8221 actgtatcgt caacaaatgg taggttttgt ttttcaagat tttaatcttt taccaacaat
     8281 gacgaataaa gaaaatataa tgatgccatt aattttagct ggtgctaaac gaaaagatat
     8341 agaacaaagg gtacatcagt tggcagtaca attacattta gagggattct taaacaagta
     8401 tccttctgaa atctctgggg gtcagaagca acgcattgcc attgcacgtg cattagttac
     8461 taaaccgacg attttactag ccgatgaacc tacaggtgca cttgattcaa aaacatcaaa
     8521 ggcattgatg atgttatttc aagagattca tcaattggaa cagacaattt taatggtaac
     8581 tcattcaaat atcgatgcgt cttatgcaga gcgagtcatt tttattaaag atgggcgtct
     8641 atatcatgaa atatatcgtg gtgaagaaag tcaattagct tttcaacaac gaataacaga
     8701 tagcttagca cttgtgaatg gaggaagtgt caatatatga agttaagatt gttatggcac
     8761 atagtgcgac gtcaatttat tatgcagcga cttgtaatca ttccattcat tttagcggta
     8821 agtgtactat tcatgattga atatacgctt gtgtcaattg ggttaaatag ctacataaaa
     8881 cagaagaatg acttcctagt accatttatt atcatagcta atttttttat ggcgctttta
     8941 acttttattt ttattttcta tgcaaatcac tttatgatgt cacaaagacg aaaagagttt
     9001 agcattttta tgacattggg catgaccaag aaaagtatgc gtttaattgt agtgatggaa
     9061 actatcttac aatttgtgat aatttcagtc gttagtattg ccggcggata cttacttggt
     9121 gcgatatttt tcttgtttat acagaaaata atgggcagtg aagttgcgac gttaaggtat
     9181 tatccatttg actctgtagc gatgtttatt actttgatta tcattgctgt attaatgggc
     9241 atgctactta tattcaactt gttcagtatt aattttcaac ggccgataac ttatcaacat
     9301 cgttccgatt ctagtgtcat atcacgatgg ttgcgttacg ttttaattgt tataggaagc
     9361 gcagcactat atttaggtta ctttattgca ttacaacaag atacgacgtt tggtgccttt
     9421 tttaaaatat ggattgtcat aggattagtt attatcggta cttatgcatt ttttgtaggt
     9481 ataagtgaaa taattattag tatattgcag caggtatcaa aagtttacta tcatccacgg
     9541 tatttttttg tggtagttgg gatgcgtgta cgtcttaaaa tgaatgcagt cagtcttgca
     9601 acaatcactt tgctgtgtac atttttgatt gtaacgctca caatgacatt aacaacctat
     9661 cgtgatatga atcataccat tacgaaattg attacgaatg attatgattt gtcatttagc
     9721 gacaattcta agtcacaaat agaacgtcaa caaacgattg agaatattaa acgtgatata
     9781 cagggttcgg ttaatgcaaa agacttcaaa gtatatgaaa cgacattatt tagagcttct
     9841 ttagaaaaga atcgtgctta ttacaagtta aaacaggcta gtgatgatat tccaatagac
     9901 ttcattggta atgaaggcgc gatattttca agacaatcag taatgattac agcttttact
     9961 gcacaagatt ataatcgata tcaccaaaac aaattacact tagacaatca aagtataggt
    10021 atgattacga atgtacctat ttttaaaaag cagtctaagg tcggtcttaa taatgaagtc
    10081 tttaaagtga aacaacttga cgcacatgcg ttaaatttga tgatgattca agatagtatg
    10141 gttttaattg ttaaagataa caatcaatta caaaaagtaa aaggatttta tgcacatgaa
    10201 caaaagaatt cgacaatatc gataaacttt aatgtatttg gaaagacgaa attaacaaca
    10261 tcacaaatac aaaagctatc gaaaaaatac gatgcgtcaa taaattctaa atcagaaatg
    10321 ctaatggttt gggatcgtct gacaggtgga ctaatatttg taggtggcgt agtatcaatt
    10381 gtacttgtca ttggaatatt tttaatgatg tactataaac aagtttcaga agcctatgca
    10441 aatcagcaca attatgacat tatgaaaaaa cttggtttag ataatggaag aatagcaaag
    10501 ataacacgaa atcaaatgac tttcttgttc gcaataccaa taacagtagc actgattcat
    10561 acattaattt ctagtaacat tgtatatacg ttactaaata tgataggtat taataaccat
    10621 catatatttt taacaagtta cgtactagct gtcattataa tttcgtttct atatatggcg
    10681 atgtacaaaa taacttccta tatttatgct aaagttattc atcaacaacg taattaacca
    10741 tgtttcag
//
LOCUS       DAHHYJ010000091        10689 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000091 DAHHYJ010000000
VERSION     DAHHYJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10689
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.091"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            50..1033
                     /locus_tag="NBK22_001800"
     CDS             50..1033
                     /locus_tag="NBK22_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCD2414984.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKISAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            1224..2150
                     /gene="pstC"
                     /locus_tag="NBK22_001801"
     CDS             1224..2150
                     /gene="pstC"
                     /locus_tag="NBK22_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCD2414985.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLSAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            2152..3069
                     /gene="pstA"
                     /locus_tag="NBK22_001802"
     CDS             2152..3069
                     /gene="pstA"
                     /locus_tag="NBK22_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCD2414986.1"
                     /translation="METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            3116..3967
                     /locus_tag="NBK22_001803"
     CDS             3116..3967
                     /locus_tag="NBK22_001803"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCD2414987.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENQITAIIGPSGCGKSTYIKTLNRMVELVPSVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            3974..4615
                     /gene="phoU"
                     /locus_tag="NBK22_001804"
     CDS             3974..4615
                     /gene="phoU"
                     /locus_tag="NBK22_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCD2414988.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IATIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(4755..6569)
                     /gene="pepF"
                     /locus_tag="NBK22_001805"
     CDS             complement(4755..6569)
                     /gene="pepF"
                     /locus_tag="NBK22_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD2414989.1"
                     /translation="MSQGLPLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHGLDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTLAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDAVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQPAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
     gene            6774..7118
                     /locus_tag="NBK22_001806"
     CDS             6774..7118
                     /locus_tag="NBK22_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414990.1"
                     /translation="MTLFDMPNYLWITTLIMILLTIFCCLVLNKWFVSAVITFVILGV
                     LAFFIPNFQDIKYQPLLGYAAFLAIMSLLISFLLWYFTRNWRKERKAHKLEKQIEKYG
                     YEGAELRRKDKK"
     gene            7066..7251
                     /locus_tag="NBK22_001807"
     CDS             7066..7251
                     /locus_tag="NBK22_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414991.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            7422..8408
                     /locus_tag="NBK22_001808"
     CDS             7422..8408
                     /locus_tag="NBK22_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414992.1"
                     /translation="MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISESSDRHA"
     gene            8401..9231
                     /locus_tag="NBK22_001809"
     CDS             8401..9231
                     /locus_tag="NBK22_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2414993.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENATKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISITVIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDALRDRSLKQRGEYDESH
                     "
     gene            9218..9991
                     /locus_tag="NBK22_001810"
     CDS             9218..9991
                     /locus_tag="NBK22_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414994.1"
                     /translation="MSLIDIQNLTIKNTCEKYLIKGIDLKIFSQQINALIGESGAGKS
                     LIAKALLEYLPFDLSCTYDSYQFDGENISRLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKSSKEEALSKIDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYIYVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            9984..10685
                     /locus_tag="NBK22_001811"
     CDS             9984..10685
                     /locus_tag="NBK22_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2414995.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLTIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLLQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLAQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQTFSY"
BASE COUNT         3675 a         1606 c         1836 g         3572 t
ORIGIN      
        1 tgtaatcaat acaaatacta tttgttgttc atacagggag gatatttcaa tgaaaaaatg
       61 gcaatttgtt ggtactacag ctttaggtgc aacactatta ttaggtgctt gtggtggcgg
      121 taatggtggc agtggtaata gtgatttaaa aggggaagct aaaggtgatg gctcatcaac
      181 agtagcacca attgtggaga aattaaatga aaaatgggct caagatcact cggatgctaa
      241 aatctcagca ggacaagctg gtacaggtgc tggtttccaa aaattcattg caggagatat
      301 cgacttcgct gatgcttcta gaccaattaa agatgaagag aagcaaaaat tacaagataa
      361 gaatatcaaa tacaaagaat ttaaaattgc gcaagatggt gtaacggttg ctgtaaataa
      421 agaaaatgat tttgtagatg aattagacaa acagcaatta aaagcaattt attctggaaa
      481 agctaaaaca tggaaagatg ttaatagtaa atggccagat aaaaaaataa atgctgtatc
      541 accaaactca agtcatggta cttatgactt cttcgaaaat gaagtaatga ataaagaaga
      601 tattaaagca gaaaaaaatg ctgatacaaa tgctatcgtt tcttctgtaa cgaaaaacaa
      661 agagggaatc ggatactttg gatataactt ctacgtacaa aataaagata aattaaaaga
      721 agttaaaatc aaagatgaaa atggtaaagc aacagagcct acgaaaaaaa caattcaaga
      781 taactcttat gcattaagta gaccattatt catttatgta aatgaaaaag cattgaaaga
      841 taataaagta atgtcagaat ttatcaaatt cgtcttagaa gataaaggta aagcagctga
      901 agaagctgga tatgtagcag caccagagaa aacatacaaa tcacaattag atgatttaaa
      961 agcatttatt gataaaaatc aaaaatcaga cgacaagaaa tctgatgata aaaagtctga
     1021 agacaaaaaa taataagacg caatttcaaa tgtgtcttga aacatgattt tgatggtgaa
     1081 tcattattta gagtacaaag cttgatttat cgagacgctg attttgacat tcagttagtc
     1141 tacaagctta tcaacttaaa atagtggttc atcattattt tacaaatcta attattttgg
     1201 gagtaataga aagaggtttg attatgactt catctactaa tgttaaagct ttaatcgaaa
     1261 aaaataataa taaaaaaggg aagcataatg acaaaattat accagttatt ttagccgcaa
     1321 tttcagcgat ttccatttta acaacactag gtatattaat cacattgctt ttagaaacca
     1381 tcactttttt caccagaatt ccaataactg aatttctatt ttctactact tggaatccta
     1441 ccggttcaga ccctaagttt ggtatctggg cattgataat agggacttta aaaatcacag
     1501 ttattgcgac tatatttgca gttccagtcg ggcttggtgc agcaatttat ttaagtgaat
     1561 atgcaagtga ccgtgcacga cgcataatta aaccgatatt agaaatttta gcaggaatac
     1621 caacaattgt gtttggtttc tttgcattaa cctttgttac accagtatta agatctttca
     1681 taccaggtct tggagagttt aatgctataa gtcccggctt agttgtcggt attatgattg
     1741 tccctctcat cacaagtttg agtgaggatg caatggcatc tgtaccaaat aaaattcgag
     1801 aaggtgccta tggacttgga gcaactaaat tagaagtagc aactaaagtc gtactttccg
     1861 cagcaacatc aggtattgta gcttcaatcg ttctcgcgat ttcaagagca attggagaaa
     1921 cgatgattgt atcattagcg gcaggtagtt cgccaacagc ttcattaagt ttaacaagtt
     1981 cgattcaaac aatgactgga tatattgttg agatagcgac aggtgatgca acatttggat
     2041 caaatattta ttacagtatt tatgctgtag ggttcacact atttatcttt accttaatca
     2101 tgaatttact ttctcagtgg atttctaagc gttttaggga ggagtattaa tatggaaacg
     2161 acagataata atagacaatc actcgtcgat caacaacttg tccaaaaaca tttatcatcc
     2221 agaacggtta aaaataaagt gttcaaactc atatttttag catgtacatt attaggactt
     2281 gtcgtactta ttgcgttgtt aactcaaaca ttgattaaag gggtaagtca tttaaattta
     2341 cagtttttca ctaatttttc ttcttcaaca ccatctatgg ctggcgttaa aggcgcgtta
     2401 atcggttcac tttggttaat gttaagtatc attccattat caatcatcct aggaataggt
     2461 acagctatat acttagaaga atatgcgaaa aacaacaaat ttactcagtt tgttaaaatc
     2521 agtatttcca atttagctgg tgtaccatca gttgtatttg ggttattagg ttacactttg
     2581 ttcgttggtg gtgcggggat tgaagccttg aaaatgggta acagtatatt ggcagcagcg
     2641 ctaacaatga ccttactgat attaccaatt attattgttt caagtcagga agcaattaga
     2701 gctgtaccta actcagtacg cgaagcttct tatggtttag gtgctaataa atggcaaacg
     2761 ataagacgtg ttgtcttacc agcagcgtta cctggtattt taactggatt cattttgtct
     2821 ctttcaagag cactgggaga aacagcgcca cttgtgctaa tcggtatacc gactatatta
     2881 ttggcaacac ctagaagtat attggatcaa ttttcagcat taccaatcca aatatttact
     2941 tgggcgaaaa tgcctcaaga agaattccag aatgttgcat cggcaggcat tatcgtttta
     3001 ctagttatct taatcttaat gaatggcgtt gcgattattt tacgtaacaa atttagtaaa
     3061 aaattctaat ttaaacaatc aatctcattt atctattaaa aagggagttt taaatatggc
     3121 gcaaacactt gcacaaacta aacaaatatc tcaaagtcat acgtttgatg tctcacaaag
     3181 tcatcataaa acaccagatg atacaaactc acattctgtt atatattcaa cacaaaattt
     3241 agacttatgg tatggcgaaa atcatgcatt acaaaatatt aatttagata tttatgaaaa
     3301 ccaaattact gccattatag gtccatctgg ttgtggcaaa tcaacatata ttaaaacttt
     3361 gaatcgaatg gttgagttag taccttctgt gaaaacagct ggtaaaatat tatatcgaga
     3421 tcaagacatt tttgatcaaa aatattctaa agaacaatta cgtacaaatg tgggcatggt
     3481 ctttcaacaa cctaatccat ttccaaaatc aatatacgat aatattactt acggtccaaa
     3541 gattcacggt attaaaaata aaaaagttct tgatgaaatc gttgagaaat cattacgtgg
     3601 cgctgcaatt tgggatgaat taaaggatag gttgcacaca aatgcatata gtttatccgg
     3661 tgggcaacaa caacgtgttt gtatcgcgcg ttgtttagca attgaacctg aagtcatttt
     3721 aatggatgaa ccgacatcag cattagatcc aatctcaaca ttaagagtag aagagttggt
     3781 tcaagaacta aaagaaaagt atacaattat tatggttaca cataatatgc aacaagcagc
     3841 tcgtgtatca gataaaactg catttttctt aaatggttat gtcaatgaat atgatgatac
     3901 tgataaaatt ttctctaacc catcaaacaa gaaaacagaa gattatattt caggaaggtt
     3961 tggttgatat ataatggcaa taattagaca gcgatatcag gagcaacttg atgatttaat
     4021 aaaggaatta cgtcggttag gtgcaaatgt ctatgtgagt attgaaaatg gtataaaatc
     4081 attaagtatt gacgatagag gctttgcacg acaaacagtt aaaaacgata aacatatcaa
     4141 tcaattaaat tatgatatta atgagcgagt tatcatgtta attacaaagc aacagcccat
     4201 tgcgagtgat ttgcgtatga tgatttcttc attaaaaatc gcctccgatt tagaaagaat
     4261 aggagataat gcatcgagta ttgccactat tcgattgcgt acaaagatta cagatgatta
     4321 tgtgttaacc cgtttaaaga caatgggtaa attagctatg ttaatgttaa aggacttaga
     4381 tcaagcattt aaaaagaaag ataccgtatt aataagagaa ataattgagc gtgatgaaga
     4441 tatcgatgac ttatatagtc atattattaa cgcaacgtat cttattgata acgatccatt
     4501 tgtcgctgca caagctcatt tagcagcaag acatttagaa cgtattggtg atcatattat
     4561 taacatcgct gaaagtgttt atttttattt aacaggtaca cattacgaac aataacttaa
     4621 agttattact ataaaatccc ttacgataaa tatatatttc tatcattcat aaaccctcaa
     4681 aaaaaccaag attctcacaa ttagtaatgt gaaaatcttg gtttatattg ttctactata
     4741 aattgtctcg catcttagtt atttgcttgc tcaatttcat ctgttaattt ttcaacttca
     4801 tcgactaaat cagaaatata ttgaattgta gatttaagtg gctgttctgt agtaatgtct
     4861 acacctgcaa tgtttgcaag ttcgacaggt gatacactac cacctttttt caatgtttct
     4921 aaccaagcat caacagctgg ttggccttca tttttaatct tttgagaaac gacagttccg
     4981 attgttaagc cagcagaata cgtatacgaa tataatccca tatagtaatg aggttgacgc
     5041 atccatgtta attcagcacc ctcagtcatg tctactgcat ctccaaaaaa ttgtttataa
     5101 acatttagca ttatttcatt taatgtcggc gcatttaaag attcaccttg atctactttg
     5161 tgatacactt cacgttgata agcagcttct aataaatggg taaccatatt atgataatat
     5221 gttctagata aaattgagcc aataacccaa cgcttaaatc ttggattatc acttgtgtta
     5281 aataaataat tggccatcaa catttcattc attgtagaag gggcttcaac aaagtacatt
     5341 gatgcttctg attcaagata tggttgatgt ttttgagcta atgtaaaatg acctgcatga
     5401 cctaattcat gtgctaagac aaatgcttca gccattttac cagtccaaga aataaacaca
     5461 tatgaatgtg taaagtatgg acttgcacaa aatgcgcctg tatctttacc tttattttgt
     5521 gcaaaatcaa tccatcgctg atcgtatgct tcacgtaaca tgtttgtata gtcatcacct
     5581 aaaacactta acgcaccgaa aatataattt tttgagtctt caattgaaat ctctggttca
     5641 taatcagggt ctacagaaat cttcaagtct tcaaaacgca tgttatctaa tccgtgaata
     5701 cgttgtaaaa gtttagcata tttctgcata actggtgcca agtcacgcat aatcatatcg
     5761 atttgacggt caaacatatc acgcgttact tcttgactat gtaataaata atcgatgact
     5821 gattcaaatc cacgtaaatc agcttcaatt ttttcttgtt gtacttgcat attatatgta
     5881 gccgcggtag tatgctgata ttttcgaatc ccatcgctaa acgatttgaa acttttacgt
     5941 ctaaactcag gatctttatt atcttcataa tcattttcaa acgtagcata gtcgacaggg
     6001 tacgttgtac catcatgttc aaatgaatcg aatgtaatat ctagcatttt tgtcgtgccg
     6061 tataaatcgt aaggactgtt tagcgtaggt gataaagttg ctaatacttg ttctaccgac
     6121 gcagataatt ggaatggctt ttgttttatt aactgtttaa tatagtgttg atatggacat
     6181 gattcttcta attgttgaag tatttcttct ggtaattcaa gtatttctga ctctacaaat
     6241 gataattggc taacaatttt accgtatgta gtagataatt tagcgctcaa tacttgtgcc
     6301 tcgatattac tagtatctac acttaaacgt agttctgcat aattacttaa gcgatctaag
     6361 gcaattaaaa tattttctaa ttcagctaaa gcagtattaa tttgttcgat tgaatttaat
     6421 gttgttgcat atgtatgatg aaattgattt gcttgttgta ctagagcgtc aatactttca
     6481 taatattgtt gatcatcttt aaataagtct actaaatccc atgtttctga aacaggaaca
     6541 tcttctctta aaggtaaacc ttgagacata taatcactcc tttggtataa tacattcatt
     6601 atacataatt ttgtctccta aacgataatg ttacgatttc catgtcgcaa ggaacaatga
     6661 tttttctaat agtagataga atattgcttg aaactgactg ctattgttta agtttttaaa
     6721 aatttataat aaaatgtata caaggaaagt aatgaaataa aaggagaatg aatatgacat
     6781 tattcgatat gcctaattat ttatggatca caacattaat aatgatttta ttaacaatat
     6841 tctgttgctt agttttaaat aaatggtttg tatctgcagt aattacattt gttatattag
     6901 gtgtgcttgc attttttatt ccaaattttc aagatataaa atatcaacca ttattaggat
     6961 acgctgcatt tttagcgatc atgagtttat taattagttt tctattatgg tattttacta
     7021 gaaattggcg taaagaaaga aaagcgcata aattagaaaa gcaaattgaa aaatacggct
     7081 atgagggcgc tgaacttcgc cgtaaagata aaaaataata ttcgacaact atatgcacaa
     7141 aatcatcgac ttttaagtta cactgcagta acaattattt cgaaagagat ggaatacagt
     7201 gctatagttg tttttgtttt gactttttta tattttacat ggttgacttg attcaaaata
     7261 ttatatttta taatgatttt gttaaaaaat taaatatact tcaatttctt tatggataat
     7321 actataatga acgttggttc ttaatatgta agttgcatat acatgcgcca ttctcatgta
     7381 aaaagaaagt gttagtaact tacacaagga gggggactat tatgttcata ataaagtcta
     7441 tgttgtatag actgatgcaa atgatagttg ttttatttgt tatttctaca cttacattca
     7501 ttttaatgaa attatcacca ggcaatcctg ttgataaaat attacatctt gatgtagcac
     7561 aagtgtcgac ggaacaaata aatgcaacca aagataaatt gggattaaat gattctttat
     7621 tggtacagtg gtggcattgg atgaatcatt tattacattt taatttagga aaaagttttg
     7681 aatcaaaaga accggtaaca cagatattat tcaactatgc acccataaca ttactcatat
     7741 cattttcaac attagtagtg tcgttatgca tatctatacc gctcggcatt atagcagcca
     7801 aacgatttca taagtggacg gataaagtga ttagagtgat ctcaacacta tcgattagtt
     7861 taccagcgtt ctttatcggt attattttat tatttattgt cacaaattta atgaatatag
     7921 atagcgttat actaagtcag tttatattac ctgtaatcac gctatcttta ggtatgtgtg
     7981 catacatcat tcgtttagtg cgttctaatt tattgatgtt attgcaaagt aatatcgtac
     8041 aagcatcaag attacgcggt atgaatgaac gttatatttt aattcatgat ttactaaaac
     8101 caacaatttt gccgattatc ccattactag ggatttcact tggcagtcta ataggtggta
     8161 ctgtagtgat tgaaaattta tttgatatac ctggtattgg ttatctatta atggatagta
     8221 ttaaatctcg agattatcct gttattcaag gatgcgtgtt atttattggc ttcttcgttg
     8281 ttattatcaa tacgattgct gatttattaa cgttattact tgatccgaag cagcgtttac
     8341 aattaggaaa tccaaaaaac aaaaccaata caccattgat atcagaaagt agtgaccgtc
     8401 atgcataaaa tattttcaaa gaataacctg atattttttg tattcgttgc atttattttt
     8461 gtggtaattg tactgcaatt ctttgtcagt agtgaaaatg caaccaaagt caatttatca
     8521 caaacttttg aacctattag ttggttgcat ttattaggaa ctgatgatta tgggagagat
     8581 ttatttaccc gaattattat cggtgcacgt tcaacattgt ttgttactgt tttaacatta
     8641 atagctatcg ttgtcatagg tgttacacta ggtctatttg ccggatataa aaaagggtgg
     8701 attgaacgat tagtgttaag gtttattgat gttggtctaa gtattccaga atttatcatc
     8761 atgattgctt tagcaagctt ttttcaacca tctttgtgga atttagttat ctcaattaca
     8821 gtaataaaat ggatgaatta cacaaggttg actagaagta tagttaatag cgaaatgaat
     8881 aagccttata taaaaatggc acaattattt catgtaccta caagaacaat attaatacgt
     8941 catttaacac ctaaaattat accggctatt atcgttttga tggtcgttga tttcggtaaa
     9001 atcattttat atataagttc actatcattt attgggttag gtgcacaacc gccaacacca
     9061 gagtggggcg ctatgttgca acaaggtcgt gattttattt cgtctcatcc aattatgttg
     9121 attgcacctg cttcagtcat tgctataact attttaattt ttaatttaac cggagatgcg
     9181 ctaagagata gatcgctaaa acaacgaggt gaatatgatg agtctcattg atatacaaaa
     9241 tttaacaata aagaatactt gtgagaaata tcttattaaa gggattgatt tgaaaatttt
     9301 tagtcaacag attaatgcct tgattggaga gagcggcgct gggaaaagtt tgattgctaa
     9361 agccttactt gaatatttac catttgattt aagctgcacg tatgattcgt accaatttga
     9421 tggagaaaat attagtagat tgagtcaata ttatggtcat acaattggtt atatttctca
     9481 aaattatgca gaaagtttta acgaccatac taaattaggt aaacagttaa ctgcgattta
     9541 tcgtaagcat tataaaagta gtaaagaaga ggctttgtca aaaattgaca aggctttgtc
     9601 gtgggttaat ttacaaagca aagatatatt aaataaatat agtttccaac tttctggggg
     9661 ccaacttgaa cgcgtttaca tagcaagcgt tctcatgttg gagcctaaat taatcattgc
     9721 agacgaacca gttgcatcat tggatgcttt gaacggtaat caagtgatgg atttattaca
     9781 gcatattgta ttagaacatg gtcaaacatt atttattatc acacataact taagtcatgt
     9841 attgaaatat tgtcagtaca tttatgtttt aaaagaaggt caaatcattg aacgaggtaa
     9901 tattaatcat ttcaagtatg agcatttgca tccgtatact gaacgtctaa ttaaatatag
     9961 aacacaatta aagagggatt actatgattg agttaaaaca tgtgactttt ggttataata
    10021 aaaagcagat ggtgctacaa gatatcaata ttactatacc tgatggagaa aatgttggta
    10081 ttttaggcga aagtggctgt ggtaaaagta cgctcgcttc attggttctt ggcttgttta
    10141 aacctgttaa aggagaaatt tacttaagtg ataatgctgt gttaacgatt ttccaacacc
    10201 ctttaactag ctttaaccct gattggacga ttgagacctc attaaaagaa gcgttatatt
    10261 attacagagg cctaactgat aatactgctc aggatcaatt attattacaa catttatcta
    10321 cttttgagtt aaacgcgcaa ttattgacta aattaccaag cgaagtgagt ggcggacaat
    10381 tacaaagatt taatgtcatg cgttcgttat tagcacagcc tcgcgtttta atatgtgatg
    10441 agataacttc aaatttagat gttatagctg aacaaaatgt aatcaatata ttaaaagcgc
    10501 aaacgattac gaacttaaat cattttatcg ttatttctca tgatttatcc gtgttacaac
    10561 gcttagttaa tagaattatt gttcttaagg atggcatgat agtcgatgat tttgcaatag
    10621 aggaattatt taatgttgat agacaccctt atacaaaaga attagtgcaa acattttcat
    10681 attagctat
//
LOCUS       DAHHYJ010000092        10681 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000092 DAHHYJ010000000
VERSION     DAHHYJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.092"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(130..810)
                     /locus_tag="NBK22_001812"
     CDS             complement(130..810)
                     /locus_tag="NBK22_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCD2414996.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILLARSSD"
     gene            complement(882..1469)
                     /locus_tag="NBK22_001813"
     CDS             complement(882..1469)
                     /locus_tag="NBK22_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCD2414997.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLILMFSHSIMAVQAIYFYPRFKRSMIGISVAMTWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(1459..2034)
                     /locus_tag="NBK22_001814"
     CDS             complement(1459..2034)
                     /locus_tag="NBK22_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCD2414998.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANI
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            complement(2048..3292)
                     /locus_tag="NBK22_001815"
     CDS             complement(2048..3292)
                     /locus_tag="NBK22_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2414999.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEEDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(3299..4597)
                     /gene="aroA"
                     /locus_tag="NBK22_001816"
     CDS             complement(3299..4597)
                     /gene="aroA"
                     /locus_tag="NBK22_001816"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCD2415000.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
                     YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSITTT
                     PDAIQHIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVSSLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(4607..5671)
                     /locus_tag="NBK22_001817"
     CDS             complement(4607..5671)
                     /locus_tag="NBK22_001817"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCD2415001.1"
                     /translation="MKLQTTYPSNNYPIFVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFVAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYD
                     LDFLKTLPFEQILSGYAEVYKHALLNGESTTQDIEQHFKDREILQSLNGMDKYIAKGI
                     ETKLDIVVADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMIGIIYQFIVANILFN
                     SNHDIQHYINYLTKLGYPLETITDIDFETIYQYMLSDKKNDKQGVQMVLIKHFGDIVV
                     QHIDQTTLQHACEQLKTYFK"
     gene            complement(5697..6863)
                     /gene="aroC"
                     /locus_tag="NBK22_001818"
     CDS             complement(5697..6863)
                     /gene="aroC"
                     /locus_tag="NBK22_001818"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCD2415002.1"
                     /translation="MRYLTSGESHGPQLTVIVEGIPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISEEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLQQLDIDIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDSIAQAMRDKIDEAKNEGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGKIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNSEIGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLQQQIADRRQLNVEF"
     gene            complement(7250..7450)
                     /locus_tag="NBK22_001819"
     CDS             complement(7250..7450)
                     /locus_tag="NBK22_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415003.1"
                     /translation="MKNIISIILGILMFLKLMELLYGAIFLDKPLNPITKIIFILTLI
                     YIFYVLVKELIIFLKSKYNKSA"
     gene            complement(7659..8108)
                     /gene="ndk"
                     /locus_tag="NBK22_001820"
     CDS             complement(7659..8108)
                     /gene="ndk"
                     /locus_tag="NBK22_001820"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCD2415004.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
     gene            complement(8200..9159)
                     /locus_tag="NBK22_001821"
     CDS             complement(8200..9159)
                     /locus_tag="NBK22_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD2415005.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQLSTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPDFKLKIEQLRRDSDRKEFEECIQIIRKSDS
                     INESKAVSSKYLSKALDLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            complement(9161..9886)
                     /locus_tag="NBK22_001822"
     CDS             complement(9161..9886)
                     /locus_tag="NBK22_001822"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCD2415006.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDRLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            complement(9889..10461)
                     /locus_tag="NBK22_001823"
     CDS             complement(9889..10461)
                     /locus_tag="NBK22_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCD2415007.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGINIDESEIYNYLFEDMSDY
                     YPSYFKKYNQSEVKHYLHDIQKSYLKSRGN"
BASE COUNT         3436 a         1981 c         1444 g         3820 t
ORIGIN      
        1 tactacaata aaataatata gtgaataatc taccattaga aaaataagca caaaaaaact
       61 agcaaccaca caaaatgtga ttagctagtt aataagtgtc taatttaagt taattgttaa
      121 tctataagat taatcacttg aacgcgcaag caaaatgata cgtacaagct ctgctacagc
      181 gacagcagtt gctgcaacat aagtcattgc tgctgcagat aatactttac gcgcatgctt
      241 gtattctttt tcatttacaa tgttcaatgc cgtaatttgt ttcatcgctc ttgaactcgc
      301 atcaaactca actggtaacg taacaattga gaataatacc gctaatgaca ttaaaccagc
      361 accaatccat aaagcagttg aaccaaatgc actacctatc gctgttaaga taatacctaa
      421 catgatgatc atataactta atgaactccc taggtttgca acaggtacta atgctgctct
      481 gaatcttaag aaccaatatc cttggtgatc ttgaatggca tgaccaactt cgtgggctgc
      541 aattgcagtt ccagcaactg atggtctgtc atagtttgca ggagatagtg aaacaacttt
      601 ctttttagga tcgtaatgat ctgttaagaa tccttcacct ttaacaactt cgacatcata
      661 aataccgttt gcatgtaaaa tttctaatgc aacttcacga cccgttttac cactagttga
      721 tctaacttgt gaatatttct catagttaga tttaactttg tgttgtgccc ataaaggaag
      781 caccattaat attacgaaat aaattatcat agtaaaaatt gaagacaata aactcactct
      841 cctttataaa tattttactg tcatttgccg tttttatcaa atcatttaca ctttaataat
      901 ttgtttaatt caatataaag caaaagtcca aaaacactca gacaacatga taatacacca
      961 atttgccaca catgtgtagt tataaaatca taatatggaa attgaaggtg aaaatagtca
     1021 atataatcat tcaaaaatac ccaagtcatc gctacactga ttccaatcat agaacgttta
     1081 aacctaggat agaagtaaat tgcctgaacg gccattatac tgtgggaaaa cattaatatc
     1141 aggccattta ctgtaatatc accttgttca ataataaata atatattcat aataactgcc
     1201 caaatcccat atttgaataa tgttacaaat gccagtgcat cgataatact attttgtttt
     1261 tgaattaata tcaatgagat agaaataact aagtataata ttgcagttgg gctatctgga
     1321 acaaaaatct taaaatgcca cggcgtatga cttaattgtt caccatacca tatataacca
     1381 taaatcatcc ctaatatatt acaaatgagt agcatcatta accaagaacg ttgataaagt
     1441 gtatattgcc aaaatgtatt aatcgtcatc tgctaagtcc tcaaattgat tatgtttatt
     1501 tactagcttg agtgtattta aaatttgcgt tagttgataa aaacgttgct tttcattcat
     1561 ctgtaaactt aaatcaatat tgtgtaacaa gtaatctatt aataacgcat gtttatgccg
     1621 atctatagcc atactattta agtcatgaag ataagtttga taactgggcg attccgttag
     1681 ttgagcaact attaaatcat ctagcctttg ctcacgcttt gacacgtttg cgaagtgaat
     1741 ttgaatatca aaagcacagt tatgattagc gatataatca aatatttcat ttgtattcat
     1801 taactttata ttacgcttag taaattgaat tgcagaagcg tgacttccca cttctgcaat
     1861 ttctaatgtt tcatgatgat taatttttgt atctacaaaa tgaatgtttg ccaatttcgc
     1921 ctcattcact tttatatagt taagcaccca aactgcaata cgcgacttaa atcgatattg
     1981 aaaaagtaaa tattcaataa aactttcttt aatttgattg agtgtctctg acatcaaata
     2041 ccccatttta agattgcaat cttgataatt cgtcatgcca attttcgtta cttggctcta
     2101 gttccaacaa ttgatttaaa atagtaattg cttgttcctt ttgaccaatt tcaattaaat
     2161 agaaataata atcactcata aaatcaatat ttgttttcat cgttggatat gctaattcaa
     2221 agaaatgttg agcttcttta tctcgctctt cttgaccata ggcgaacgct aaatgccaca
     2281 tgaatgtagg gtccaaatct tcttcatcta catatgttaa taattcaatg attgcttcat
     2341 aatcttcttc attacgatat aaatcgctta aaatcaataa aggttcttgg taagcattat
     2401 caacctctaa tgcttgtttt aacaataata caccttcatt agcatcgccg tgttctattt
     2461 ccaaacatcc agttgtatac attaactctt tataaaattg acttagtcgt aatccttctt
     2521 tacccgtctc aatggcatct ggataatttt tttcattttc atataatgat tgtaaataca
     2581 agtagccttg aatataatca ggatctttag aaagtaatgt agtcattatt ttaattgctt
     2641 cttgagtgat gtcattttta tcgtaagaaa tggctttttt gagataatct tctgaagtca
     2701 tttcatcttc attaatttca tcgtataagc gaatcgcatc actatagtta ccactttgta
     2761 aactacaatc tgccatacga gagaataagt ttacaccatt aacttgatat tcaccagttt
     2821 ctaaaacggt ttcgtattca gaggtagcac gtaaatattg accatcataa tataacattt
     2881 cagccaatgc aaaatggatt attggatcat ttggctctag ttcaagtgct tcttgtaatt
     2941 tatcaatagc aacttccatc atattaattt gttgatataa atctgcttct aacatcaact
     3001 tttcaggtga tggttcaaca taacttaaat attctaacgc ttcgtcagtt tgattttcag
     3061 acattaaacc ttcaataaaa taaatcagca attcactttc gtctggatat ttgtgatata
     3121 acacgcggaa tacttccaaa ccttgtggca ttaatccaaa attgtaaagt gtctctccta
     3181 gaataaataa tgcgtcatcg ttgtcagtag ttattgcttc attaacacga gagtctaaat
     3241 tttctagttt ttgtagattg atatcgtcta ttaatttata gatatcttcc attttatatt
     3301 atccctcatt ttctaaaagc tttagttttg gtaaaaatcc tggaaatgat acatttacag
     3361 catcaaattg tttgattttg acaggctcgc ttgaaagtag agaagaaact gcaagcatca
     3421 ttcctattcg atgatcagtt aaactatcaa ctgttgcatt tgttttaaat tctgacggat
     3481 gaataatcaa tccatcatta gttggttgta attcaaaccc taacaagttt aacatatcag
     3541 ccgttgtatc aattctattt gtttctttta cttttaattc ctcggcatct ttaattgtac
     3601 tcgtgccaac tgcttgtgta caaagtaatg ctattacagg cagttcatca attgcttttg
     3661 gcactaattc tccttcgatt gttattggtt gaagcattgg tgtgtattga atacgaatag
     3721 aagcagtagg ttcagcacca gttgtttgat tgaaaagttg gatattaccg cccatttttt
     3781 caacaatatc aataatacct gaacgtgttg gattgattcc aacattatga attgttacat
     3841 cacttcctgg tgtgataagt gctgcaacaa taaagaacgc tgcagatgaa atatcgccag
     3901 gaacatgaaa atctgcaggt ttaatatgtt gaatggcatc aggggttgta gttattgata
     3961 acccttctgc ttcaattgga atattaaaat gtttgaacat cgtctcagta tgatttcgac
     4021 ttacatctaa ttctttaatg atggtcggtt ccttagaaaa caaacttgca aataaaatgg
     4081 cacttttcac ttgtgcactt gcaacttcca tttgataatt tatacctttt atgacagatg
     4141 gcttaataat taatggtgta taattatctt caataccttc aatattcgca tccataagtt
     4201 tcaatggtct caagacacga tccattggcc ttttaccaat tgaaacatcg ccagacaaaa
     4261 cactttcaat acctaaacca cttaacaaac cagctaataa tcgtgttgtc gtaccagaat
     4321 tacctgtata caatacttga tgtggcgtgt taaaagattg atatcctggg gaagtcacaa
     4381 ctaatttttc atcatcttct ttgatttcta cacctaacag tcggaaaatg tccatcgtac
     4441 gacgacaatc ttcgccaagt agtggcttat atatagtaga tacaccttca gctagcgacg
     4501 ccaacatgat tgcacggtgt gtcattgact tatcgcccgg cacttctatt tcgcccttta
     4561 acggacctga aatatcaatg atttgttcat ttaccatttc attcacctac ttaaaatatg
     4621 tttttagttg ttcacacgca tgttgcagtg ttgtttgatc aatgtgttgc actacaatat
     4681 cgccaaaatg tttaatcaat accatttgaa ctccttgttt gtcgttcttt ttatcactta
     4741 gcatgtattg atatatcgtt tcgaaatcta tgtcggttat tgtttctaaa ggataaccta
     4801 atttcgttaa ataattaata taatgttgga tatcgtgatt agaattgaac aatatattcg
     4861 caacaataaa ttgatatatt atgcctatca ttacggcatg accatgaggt attttatgat
     4921 agtattcaac agcatgccca aatgtatgac ctaaatttaa aaatttacgt acaccttgtt
     4981 ctttttcatc tgcaacaaca atatccagct tcgtttcaat acctttagca atatatttat
     5041 ccataccatt taatgactgt aatatctctc tatctttaaa gtgctgttcg atatcttgcg
     5101 tcgttgattc accattcaat aacgcatgct tataaacttc tgcatagcca cttaatattt
     5161 gctcaaatgg taacgtcttt aaaaaatcta aatcataaat cacagcagtt ggacgataaa
     5221 atgcaccgat aaggttttta ccttgttttg agttaatacc cactttaccg ccaacactag
     5281 aatcatgcgc caaaatcgtt gtaggaactt gtataaaatg gacacctctt aatagtgttg
     5341 ctgctacaaa tcctgcaaaa tctcctgtcg caccaccacc aacagctata atcgctgtat
     5401 tacgcgttac atgatgtgac aaaatgtatt ctaatgtttc ttgatattgc tcaaatgttt
     5461 tcgtcttttc accagctgga ataatgactt tatgcacatt ttcatatgat aaaatatcat
     5521 caaatttatc agcaaaatat tgatttacat gctcgtcaat taatataaaa ctttgatcaa
     5581 actgatcaat atacgtgcta atatggtcaa ttgcaccatg ttcaacaaat attggataat
     5641 tgtttgaagg ataggttgtt tgtaatttca tgatcacacc tcaattgttc tcgttgttaa
     5701 aactcaacat ttaattgtct gcggtcagca atttgttgtt gaagttgttc aatatggttt
     5761 gattgaaact cttcaagtaa tgctttcgct atttcaaatg caacaacgtg ttcacacact
     5821 atacttgctg caggtacagc acaactatca gaacgttcaa ttgttgcctt aaagtcttct
     5881 ttagtattta tatcaactga atttaatggt ttatataacg taggaatagg tttcattaca
     5941 ccatttacaa taattggcat accatttgac ataccaccct ctaaaccacc taaatgatta
     6001 gatccacgat aataaccaat ttcactatta tatagaattt catcttgaat ctcactacct
     6061 ggcttttcag ctgctttaaa tccttcacca aagcttacac ctttaaaagc atttatgctg
     6121 acaacacctt gtgcaatctt accatctaac ttacgatcat aatgcacata actaccaaca
     6181 ccaacaggca tattttcaac tacaacttga acgacaccgc caattgaatc tccttcattt
     6241 ttagcttcgt caattttatc tcgcattgct tgtgcgatac tgtcatcaat tacacgaaca
     6301 tcattacgat caagatttgc tttaaatgtt tctgaatcat aaaaatcttt atctttaatt
     6361 ccacctattt caacaacacg actgtatata tcgatatcta actgttgtaa taacacttta
     6421 cataaggcac cgactgcaac tcgagctgct gtttctctag cagatgatcg ctctagcaca
     6481 tttcgtaaat cacgatgatt atatttcata cctccaacca aatctgcatg accaggtctt
     6541 ggttttgtaa tagtacgttt catattttca cgttcttctt cacttattgg agctgctccc
     6601 ataatttttc tccaatgcgt aaagtcatca ttggttacaa ccatagtaat tggactacct
     6661 aatgtataac catttctaac gcctgatact atttctactg tatctttctc aatttgcatg
     6721 cgtcggccac gaccataacc cccttgacgc ttgaacattt ctttattaat atcttcaact
     6781 ttaatttcta aatttgctgg tataccttcg acaataactg ttaattgagg tccatgtgat
     6841 tctcctgaag ttagatatct catacacact cgctcctttc atacttccgc aataacatta
     6901 taatatgttg ttaatcttat cacattcatt gtggattcat aaatgtttgt tttcaagctt
     6961 aattaagaaa atggatgaca tctcatttta aatgtcagaa ttatcttaat attaaacgaa
     7021 ctaaaattaa ttataatata ttcgtctgta gaaaagtatc atgaatttct aaacagttgc
     7081 atcatataat cattaattaa aaatgattag ccgttttaat ataatcgtat cgcaacttta
     7141 gcaaataata tactgactac atttcttaat acaaaagaac gaactgttaa ttattaataa
     7201 agttaatcag tccgtttaaa aaaaattatg atattaaaat atacatatgt taagcgcttt
     7261 tgttatactt tgacttcaaa aatataatca attcttttac taatacataa aaaatataaa
     7321 tgagagtcag tataaaaata atttttgtta taggattaag tggtttatct aaaaatatag
     7381 caccatatag taattccatt aattttaaga acattaaaat ccccaaaata atcgaaataa
     7441 tattcttcat tttaacaacc ccttctctct gtttagtttc atttgataaa gaatattcct
     7501 ctctttttaa tacgtgttac caaaaaacta taaaattttt caagtaattt actacaacat
     7561 aaaaaacggg ccttgttatg tataatcata atgactaacc acataacaaa accctttcta
     7621 cctttataaa taaatcgtaa aggtttacag tttatatttt attcatataa ccatgcatca
     7681 cgtggtgaag catagctagt aatttcattt tcattaaacc atagattaat ttcacgttca
     7741 gcagactcta atgaatctga accgtgaatg atatttctac caacagttaa acctaaatca
     7801 cctctaattg atcctggtga agcttctgaa ggattggtgc tgccaataat atgtctagat
     7861 acattaactg catcttcacc ttcaactacc attgcgaaca ctggtgctga tgtaataaat
     7921 gaaattaaat cattataaaa cggtttgcct tggtgttcac cataatgtgt ttcagcaagt
     7981 tccattggta cttgcattaa tttaccaccg acaagtttta gtccttttct ttcaattctt
     8041 gaaattactt caccaattag atttctttgt actgcatctg gtttaatcat taaaaatgta
     8101 cgttccactt tatgtatccc cctgtgttta ataggtttat gtatattact attatatatt
     8161 aacaggttgg taaagcgctt tcaatacttt tcataaatac tacgtgtttc ttgaacccat
     8221 ttttttcgtc aaacttaaaa gtagtgattt cggatgtcca tctggtaact cagaaatcaa
     8281 atccaaagct ttacttaaat acttcgaact tactgcttta gattcattaa tactgtcaga
     8341 ctttctaata atttgaatac attcttcaaa ttcttttcga tcgctgtcac gtcgtaattg
     8401 ctcaattttc aatttgaagt ctgggttttt acgcatttct aataaaatcg gtaacgtaat
     8461 atgaccatta agcaaatcac ttccgaccgg cttacctaat ttcttttcgg tacttgtgaa
     8521 gtctaataca tcatcaatga tttggaagct catacctata taatgaccaa tcattttcaa
     8581 ttttcgtaca gtctctttat cagattgaga tgtaattgca ccaacttcag ttgataattg
     8641 aattaacagt gctgttttgc gattgatacg tcgtaaataa ttgataattg tctgttgact
     8701 gttaaattgg tcttgaaatt ggaaaagttc ccctctacaa acatcaacga tagattcaga
     8761 tattaattga tgtacacgat tatctttaac ggccattaag tgttcaagtc ctaatgccaa
     8821 taaaaaattc ccagttaaaa tagccgttgt ctgatcccat ttctttgata tggttaactt
     8881 tcctcgacgc ttgtcgcttt tatcaataac gtcatcatga acaagtgttg ccatatgaat
     8941 taactctaat gcgactgcaa cttgatacgt ttgttcagac gtttgttcat ctttgccaaa
     9001 ttggctactc agaattacaa atgctgggcg tactcttttg ccaccagaag ataataagtg
     9061 taatgatgcc tgttctaata cagaatcttt actttttatt gccttttcaa gtcgttgttc
     9121 cactttctta atttcattgt tcatgtttaa ctttgccacg ttaatcacct ttggtattat
     9181 ctttttcttt atagccaagg tgcattgcag caacgccccc tgtaaaacta cgtactctta
     9241 catttatgaa acccgcttct tcaaacatgc gcttcaactc ttcttttcca ggaaaattaa
     9301 acgtagattg ctgtaaccat tcatattctt cttttgattt tgcaaataat tttccaaaaa
     9361 taggcataac aaatttaaag tataatgcat acatttgttt aaagactggc aaagttggtt
     9421 ggctcgtttc aagacatacc accataccac ctggtttaag tactctattc atttctttta
     9481 acgcgactaa atagtctggc acatttctta atccaaaccc aattgttaca taatcaaaag
     9541 aattgtcttc aaacggcaat tccattgcat caccatgaac aagtttaaca ttttccattg
     9601 aagcagtttt ttcttttcct acttctaaca tattctcact aaagtcaata ccagtaactt
     9661 cacctgttgg tcctacagct ttgcttaatg cgattgtcca atcaccagta ccacaacaaa
     9721 catctaatgc tttcgtccct tttctaacgc ccatgtcttt catgacgcgt tttctccata
     9781 ctttatgctg ctcaaaacta ataatattat ttaatctatc atattttttt gaaatatttt
     9841 gaaaaacgcg atgtacttgc tctttatttg ctttattgtc agccatgctt aattacctct
     9901 actttttaaa taactttttt ggatatcgtg taagtaatgc tttacttcac tttgattata
     9961 tttcttgaag tatgatggat agtaatcaga catatcttca aataaataat tatatatttc
    10021 cgactcatca atattgatac cgaaatgaga taacgtaata tatgggaaaa gtgtttcaat
    10081 ttttactatt gcttgagaaa tttcataatc atttaaagct tgatgatgta atgaagattt
    10141 caattcatta atttcaacaa ttgctttact aatttcattt tgaaatgata aatcattgat
    10201 ttctgctagt agcgtataaa aatgtgcact aattaaatct ccaatcaaaa tggaatgttt
    10261 agacaaatga ttatatgtaa tgtcatcaag gtgtctcatt gatgtgtcaa ttgtgaggca
    10321 tgctactttg gcaacatctg gaatatcata tgaatcaagt aacttaccta accgatgatt
    10381 aatattaatg gattcatatt ctgatacacc ctttaatctt tcttctattt gtctttccaa
    10441 tttgctaaca gttgtttcca tgtctacacc tcacgatatt atcactattc atattaacat
    10501 tatatgtaag aaattaaaat cttttgaagc attaagatta cttatcattt ttaaatttca
    10561 atttaaacta acagtaattt atgtagcttt tgtaattctc ataataacct ttacttcatt
    10621 tacattattc atctttttaa ataacgtaaa atactatagt agacatggtt accttttaag
    10681 t
//
LOCUS       DAHHYJ010000093        10560 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000093 DAHHYJ010000000
VERSION     DAHHYJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.093"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            16..1092
                     /locus_tag="NBK22_001824"
     CDS             16..1092
                     /locus_tag="NBK22_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD2415008.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            1157..1468
                     /locus_tag="NBK22_001825"
     CDS             1157..1468
                     /locus_tag="NBK22_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD2415009.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            1568..2425
                     /locus_tag="NBK22_001826"
     CDS             1568..2425
                     /locus_tag="NBK22_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCD2415010.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            2454..3050
                     /locus_tag="NBK22_001827"
     CDS             2454..3050
                     /locus_tag="NBK22_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCD2415011.1"
                     /translation="MNLFYNPKFVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            3071..6895
                     /locus_tag="NBK22_001828"
     CDS             3071..6895
                     /locus_tag="NBK22_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD2415012.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSVDIISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYEAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSELTDNSTDASQLHTNGIENETVSNVENKQASIQNEDTNDSHVDES
                     QYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNKNASEMHVDAPKTQEY
                     AVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDGHVVEDSSMNEIEK
                     NNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVPELKPVQSKKAVSESKPASQAAPSSSSDSQESNTNAYKTNNITSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIEPDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            6919..8232
                     /locus_tag="NBK22_001829"
     CDS             6919..8232
                     /locus_tag="NBK22_001829"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCD2415013.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKLDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFDNAVVLFMGAGDIQKLQNAYLDKLGMKNAF"
     gene            8306..8797
                     /locus_tag="NBK22_001830"
     CDS             8306..8797
                     /locus_tag="NBK22_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCD2415014.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            8871..10076
                     /locus_tag="NBK22_001831"
     CDS             8871..10076
                     /locus_tag="NBK22_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415015.1"
                     /translation="MTQQQNNKRTLKNKHTYQNEPLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAFTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEESKAAALKAQQAAI
                     KEEASANNLSDTSQEAQEIQEAKKEAQAETDKSVAVSNEESKAVALKVSKEESQAERL
                     ANAAKQKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLVTKKNDVSESETVNIDN
                     KDTVKQKEAKFENGVITRKADEKTTNNTAVDKKSGKQSKKTTPSNKRNASKASTNKTS
                     GQKKQHNKKSSQGAKKQSSSSKSTQKNNQTSNKNSKTTNAKSSNASKTPNAKVEKANS
                     KIEKRTFND"
     gene            10232..10315
                     /locus_tag="NBK22_001832"
     CDS             10232..10315
                     /locus_tag="NBK22_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415016.1"
                     /translation="MLFVTSLDYNSDIAHFKLKMEKYSIIK"
BASE COUNT         4168 a         1443 c         1957 g         2992 t
ORIGIN      
        1 ggagtgagaa cttttatgaa cataaataaa aaagtaacat tacaacgaat tcaaacttta
       61 actgagcttc atggggcacc aggttttgaa gaagaagtaa aaaattatat gactcagcaa
      121 atggcgccgt acgtagatga atttattgaa aatcgtatgg gtggattttt tggtgtgaaa
      181 aaatctaaaa atccaaatgc aaaacgtgta atgattgcag cacatatgga tgaaatcgga
      241 tttatgatta caaatatcac taaaaatgga atgattcaat tcacaaattt aggtggtgtt
      301 gcaaatgata tttggcaagg acaacgctta gtaattaaaa atagaaatgg cgataaaatt
      361 atcggtgttg tttctaatat acctaaacat tttcgtactg gtagtgaagg tgcaccggaa
      421 attaaagatt taacattaga tataggtgct caaaatgaag atgaggtgcg tgagcgcgga
      481 atagatatag gagatacaat tgtacctcac acgccattca cacagttatc tgaacatcga
      541 tatagtgcta aagcatggga taatcgttat ggttgtgtct tggcaattga aatactagaa
      601 ttattaaaag atatagaatt agatgtagac ttgtatgttg gcgcaaatgt tcaagaagag
      661 gttggattac gaggtgcgaa agcatctgca gagatgatag acccagacgt tgcatttgta
      721 gttgattgtt cacctgccaa tgacgttaaa ggaaaccaac cattatctgg tgaacttggt
      781 aaagggacgt taattcgcat aaaagacggt acaatgattt taaagcctgt atttagagac
      841 tatttattaa agttagtaga agcacatgac attgaacatc aatactatat gtcaccaggt
      901 ggaacagatg gtggagaaat tcataaagct aatattggta ttccgactgc agttattggt
      961 gtatgtgcac gatatattca tagtacagac tcagtatttg atataagaga ctattttgca
     1021 gctagatctt tactttcaga agccatttgt aatttagata ataatcaaat agaaacatta
     1081 caatataaat aatcgggtaa taacaactat tatctctaaa tagttatata taatcattaa
     1141 ttaaggagac ataaaaatga aacaacttga atcagaacaa caatttgaat ctttaaaaca
     1201 aggtgctaca gtatttgaat tcactgcagg ctggtgtcca gattgtagag tgatagaacc
     1261 agatttaccg gaattagaag cgagatatcc tatgtttgac ttcgtatcag tagaccgtga
     1321 taaatttatg gatatttgta ttgaaaatgg tattatgggt attccaagtt ttctagtata
     1381 taaaaatgga gaactgcttg gaagttatat tggaaaagaa cgaaaatcaa ttgaacagat
     1441 agatgcattt ttagctcaat acgtgtaatt tagactagag aaaaacgggg taatactcgt
     1501 ttttctctgt tactatgtgt tgatttattg taaactatta taaggtgcga aattaggagt
     1561 gttacatatg aatacctttc aaatgagaga taaattaaag gaacgtttaa gccatttaga
     1621 cgttgatttt aaatttaatc gtgaagaaga aactttgcgt atttatcgaa cagataataa
     1681 caaaggtatc acgattaaac ttaacgctat agtcgcaaaa tatgaagata aaaaagaaaa
     1741 aattgtagat gaaattgttt attacgttga tgaagctatt gcacaaatgg cagataaaac
     1801 ccttgagagt atatcatcta gtcaaattat gcctgtcatt agagcgacta gctttgataa
     1861 aaaaactaaa caaggtgttc cttttatcta tgatgagcat actgcagaaa cagcagttta
     1921 ttatgcagtc gatttaggga aatcatatcg tctaattgac gaaagcatgt tagaggattt
     1981 gaagttaact gaacaacaaa taagagaaat gtctctgttt aatgttagaa aattgtcaaa
     2041 ttcatatacg actgatgaag taaaaggtaa tattttttat tttattaact caaatgacgg
     2101 gtatgatgca agtaggatac taaatactgc atttttaaat gaaattgagg cacaatgtca
     2161 aggcgaaatg ctcgtagcag tgccacacca agatgtgtta attattgcag atatacgcaa
     2221 taaaacagga tatgatgtga tggcacattt aacaatggaa tttttcacta aaggtctagt
     2281 tccaattaca tcattatcct ttggatataa acagggtcat cttgaaccga tatttatttt
     2341 aggtaaaaat aataaacaaa aaagagatcc aaacgtgatt cagcgtttag aagcaaatcg
     2401 tcgtaaattt aataaagata aatagaaata attggataag gagttttgtc ataatgaatt
     2461 tattttacaa tcctaaattt gtaggagatg tcgcattttt acaaattgaa ccagttgaag
     2521 gtgaattaaa ctacaataaa aaaggtaatg ttgttgaaat tactaatgaa ggtaatgttg
     2581 taggttataa tatttttgaa atttcaaaag atataacaat tgaagaaaaa ggtcatatta
     2641 aattaactga tgaacttgta aatgtattcc aaaagcgtat ttcagaagct ggttttgatt
     2701 ataaattaaa tgctgatcta tcaccgaaat ttgtagttgg ctacgttgaa actaaagaca
     2761 aacatcctga tgcagataaa ttaagtgtac taaatgtaaa cgttggaaat gacacattac
     2821 aaattgtatg tggcgcgcct aacgttgaag ctggacagaa agttgttgtt gctaaagtag
     2881 gtgcagtgat gcctagcggt atggtaatta aagatgctga attacgtggt gttgcctcaa
     2941 gcggtatgat ttgttcaatg aaagaattga atttacctaa tgcacctgaa gaaaaaggta
     3001 ttatggtatt aaatgacagc tatgaaattg gacaagcatt ttttgaataa ttaaggaagg
     3061 tagtgaaaat atgagctggt ttgataaatt attcggcgaa gataatgatt caaatgatga
     3121 cttgattcat agaaagaaaa aaagacgtca agaatcacaa aatatagata acgatcatga
     3181 ctcattactg cctcaaaata atgatattta tagtcgtccg aggggaaaat tccgttttcc
     3241 tatgagcgta gcttatgaaa atgaaaatgt tgaacaatct gtagatatta tttcagatga
     3301 aaaagaacaa taccatcgag actatcgcaa acaaagccac gattctcgtt cacaaaaacg
     3361 acatcgccgt agaagaaatc aaacaactga agaacaaaat tatagtgaac aacgtgggaa
     3421 ttctaaaata tcacagcaaa gtataaaata taaagatcac tcacattacc atacgaataa
     3481 gccaggtaca tatgtttctg caattaatgg tattgagaag gaaacgcaca agtcaaaaac
     3541 acataatatg tattctaata atacaaatca tcgtgctaaa gattcaactc cagattatca
     3601 caaagaaagt ttcaagactt cagaggtacc gtcagctatt tttggcacaa tgaaacctaa
     3661 aaagttagaa aatggtcgta tccctgtaag taaaccttca gaaaaagttg agtcagataa
     3721 acaaaaatat gataaatatg aagctaagac gcaaacgtct caaaataaac aattagaaca
     3781 agagaaacaa aatgatagtg ttgtcaaaca aggaactgca tctaaatcat ctgatgaaaa
     3841 tgtatcatca acaacaaaat caatgcctaa ttattcaaaa gttgataata ctatcaaaat
     3901 tgaaaatatt tatgcttcac aaattgttga agaaattaga cgtgaacgag aacgtaaagt
     3961 gcttcaaaag cgtcgattta aaaaagcgtt gcaacaaaag cgtgaagaac ataaaaacga
     4021 agagcaagat gcaatacaac gtgcaattga tgaaatgtat gctaaacaag cggaacgcta
     4081 tgttggtgat agttcattaa atgatgatag tgaattaaca gataatagta cagatgctag
     4141 tcagcttcat acaaatggca tagagaatga aactgtatca aatgttgaaa ataaacaagc
     4201 gtcaatacaa aatgaagaca ctaatgactc tcatgtagat gaaagtcaat acaattatga
     4261 ggaagttagt ttgaatcaag tatcgacaac aaaacaattg tcagatgatg aagttacggt
     4321 ttcgaatgta acgtctcaac atcaatcagc actacaacat aacgttgaag taaatgataa
     4381 agatgaacta aaaaatcaat ctagattaat tgctgattca gaagaagatg gagcaacgaa
     4441 taaagaagaa tattcaggaa gtcaaatcga tgatgcagaa ttttatgaat taaatgatac
     4501 agaagtagat gaggatacta cttcaaatat cgaagataat accaataaaa acgcgtctga
     4561 aatgcatgta gacgctccta aaacgcaaga gtacgcagta actgaatctc aagtaaataa
     4621 tatcgataaa acggttgata atgaaattga attagcaccg cgtcataaaa aagatgacca
     4681 aacaaactta agtgtcaact cattgaaaac gaatgatgtg aatgatggtc atgttgtgga
     4741 agattcaagc atgaatgaaa tagaaaagaa taacgcagaa attacagaaa atgtgcaaaa
     4801 cgaagcagct gaaagtgaac aaaatgtcga agagaaaact attgaaaacg taaatccaaa
     4861 gaaacagact gaaaaggttt caactttaag taaaagacca tttaatgttg tcatgacgcc
     4921 atctgataaa aagcgtatga tggatcgtaa aaagcattca aaagtcaatg tgcctgaatt
     4981 aaagcctgta caaagtaaaa aagctgtgag tgaaagcaag cctgcgagtc aagccgcacc
     5041 atcatcaagc tctgattcac aagagtcaaa cacaaatgca tataaaacaa ataatattac
     5101 atcaaacaat gttgagaaca atcaacttat tggtcatgca gaaacagaaa atgattatca
     5161 aaatgcacaa caatattcag agcagaaacc ttctgctgat tcaactcaaa cggaaatatt
     5221 tgaagaaagc caagatgata atcaattgga aaatgagcaa gttgatcaat caacttcgtc
     5281 ttcagtttca gaagtaagcg acataactga agaaagcgaa gaaacaacac atccaaacaa
     5341 tactagtgga caacaagata atgatgatca acaaaaagat ttacagcctt cattttcaaa
     5401 tcaaaatgaa gatacagcta atgaaaatag acctcggacg aaccaacaag atgttgcaac
     5461 aaatcaagct gtacaaacat ctaagccgat gattcgtaaa ggcccaaata ttaaattgcc
     5521 aagtgtttca ttactagaag aaccacaagt tattgagccg gacgaggact ggattacaga
     5581 taaaaagaaa gaactgaatg acgcattatt ttactttaat gtacctgcag aagtacaaga
     5641 tgtaactgaa ggtccaagtg ttacaagatt tgaattatca gttgaaaaag gtgttaaagt
     5701 ttcaagaatt acggcattac aagatgacat taaaatggca ttggcagcga aagatattcg
     5761 tatagaagcg cctattccag gaactagtcg tgttggtatt gaagttccga accaaaatcc
     5821 aacgacggtc aacttacgtt ctattattga atctccaagt tttaaaaatg ctgaatctaa
     5881 attaacagtt gcgatggggt atagaattaa taatgaacca ttacttatgg atattgctaa
     5941 aacgccacac gcactaattg caggtgcaac tggatcaggg aaatcagttt gtatcaatag
     6001 tattttgatg tctttactat ataaaaatca ccctgaggaa ttaagattat tacttatcga
     6061 tccaaaaatg gttgaattag ctccttataa tggtttgcca catttagttg caccggtaat
     6121 tacagatgtc aaagcagcta cacagagttt aaaatgggcc gtagaagaaa tggaacgacg
     6181 ttataagtta tttgcacatt accatgtacg taatataaca gcatttaaca aaaaagcacc
     6241 atatgatgaa agaatgccaa aaattgtcat tgtaattgat gagttggctg atttaatgat
     6301 gatggctccg caagaagttg agcagtctat tgctagaatt gctcaaaaag caagagcatg
     6361 tggtattcat atgttagtag ctacgcaaag accatctgtc aatgtaatta caggtttaat
     6421 taaagccaac ataccaacaa gaattgcatt tatggtatca tcaagtgtag attcgagaac
     6481 gatattagac agtggtggag cagaacgctt gttaggatat ggcgatatgt tatatcttgg
     6541 tagcggtatg aataaaccga ttagagttca aggtacattt gtttctgatg acgaaattga
     6601 tgatgttgtt gattttatca aacaacaaag agaaccggac tatctatttg aagaaaaaga
     6661 attgttgaaa aaaacacaaa cacaatcaca agatgaatta tttgatgatg tttgtgcatt
     6721 tatggttaat gaaggacata tttcaacatc attaatccaa agacatttcc aaattggcta
     6781 taatagagca gcaagaatta tcgatcaatt agagcaactc ggttatgttt cgagtgctaa
     6841 tggttcaaaa ccaagggatg tttatgttac ggaagcagat ttaaataaag aataattatg
     6901 agtaaggagt tttatataat gacacactat cattttgtcg gaattaaagg ttctggcatg
     6961 agttcattag cacaaatcat gcatgattta ggacatgaag ttcaaggatc ggatattgag
     7021 aactacgtat ttacagaagt tgctcttaga aataagggga taaaaatatt accatttgat
     7081 gctaataaca taaaagaaga tatggtagtt atacaaggta atgcattcgc gagtagccat
     7141 gaagaaatag tacgtgcaca tcaattgaaa ttagatgttg taagttataa tgatttttta
     7201 ggacagatta ttgatcaata tacttcagta gctgtaactg gtgcacatgg taaaacttct
     7261 acaacaggtt tattatcaca tgttatgaat ggtgataaaa agacttcatt tttaattggt
     7321 gatggcacag gtatgggatt gcctgaaagt gattatttcg cttttgaggc atgtgaatat
     7381 agacgtcact ttttaagtta taaacctgat tacgcaatta tgacaaatat tgatttcgat
     7441 catcctgatt attttaaaga tattaatgat gtttttgatg cattccaaga aatggcacat
     7501 aatgttaaaa aaggtattat tgcttggggt gatgatgaac atctacgtaa aattgaagca
     7561 gatgttccaa tttattacta tggatttaaa gattcggatg acatttatgc tcaaaatatt
     7621 caaattacgg ataaaggtac tgcttttgat gtgtatgtgg atggtgagtt ttatgatcac
     7681 ttcctgtctc cacaatatgg tgaccataca gttttaaatg cattagctgt aattgcgatt
     7741 agttatttag agaagctaga tgttacaaat attaaagaag cattagaaac gtttggtggt
     7801 gttaaacgtc gtttcaatga aactacaatt gcaaatcaag ttattgtaga tgattatgca
     7861 caccatccaa gagaaattag tgctacaatt gaaacagcac gaaagaaata tccacataaa
     7921 gaagttgttg cagtatttca accacacact ttctctagaa cacaagcatt tttaaatgaa
     7981 tttgcagaaa gtttaagtaa agcagatcgt gtattcttat gtgaaatttt tggatcaatt
     8041 agagaaaata ctggcgcatt aacgatacaa gatttaattg ataaaattga aggtgcatcg
     8101 ttaattaatg aagattctat taatgtatta gaacaatttg ataatgctgt tgttttattt
     8161 atgggtgcag gtgatattca aaaattacaa aatgcatatt tagataaatt aggcatgaaa
     8221 aatgcgtttt aatatgttta taatagagta gtatgggtat ttattattaa tgacattatt
     8281 acatgttaat taggaggcgt ttttaatgga ttggatttta ccaattgctg gaattatcgc
     8341 tgcgattgca ttcttaattt tatgtatcgg tatcgtagct gtattaaatt ctgttaagaa
     8401 aaacttagat tatgttgcaa aaacacttga cggtgtagaa ggtcaagttc aaggtattac
     8461 tcgtgaaaca acagatttac ttcataaagt aaaccgttta actgaggata tccaaggtaa
     8521 agtagatcgt ttaaactcag ttgtagatgc tgttaaaggt atcggtgact cagtacaaac
     8581 gttaaacagc tctgtagatc gtgtaacaaa ttcaattaca cataatattt ctcaaaatga
     8641 agataaaatc tcacaagttg ttcaatggtc aaatgttgca atggaaattg cagacaaatg
     8701 gcaaaataga cactaccgtc gtggaagtgc aaattacaaa gctaataatg tagcaactga
     8761 tgcaaatcat agctatactt ctcgagtaga taaataatta ataattaaac aaatgcactt
     8821 aaatgaggtt gttacttatt taagtgcatt tgttatagga ggatacacaa atgacgcaac
     8881 aacaaaataa taaaagaaca ttaaaaaata aacacactta tcaaaatgaa ccattaccca
     8941 accgtaaaga ttttgttgtt agttttataa ctggcgcgct tgttggttca gctttaggtt
     9001 tgtattttaa aaataaagtt tatcaaaaag cagatgattt aaaagtcaaa gaacaagaac
     9061 tgtcgcaaaa atttgaagaa agaaaaacgc aacttgaaga aacggttgcc tttacaaaag
     9121 aacgtgttga aggattttta aacaaatcta aaaatgagca agcagcatta aaagcacaac
     9181 aagcagcaat aaaagaagaa gcaagtgcaa ataatttgag tgatacatca caagaggcac
     9241 aagagattca agaagctaaa aaagaagcac aagcagaaac agataaaagt gcagctgtat
     9301 caaatgaaga atcaaaagca gcggcattaa aagcacaaca agcagcaata aaagaagaag
     9361 caagtgcaaa taatttaagt gatacatcac aagaggcaca agagattcaa gaagctaaaa
     9421 aagaagcaca agcagaaaca gataaaagtg tagctgtatc aaatgaagaa tcaaaagcag
     9481 tggcattaaa ggtatcgaaa gaagagtcac aagctgaaag attagcaaac gctgcaaaac
     9541 agaagcaagc taaattaaca ccaggttcaa aagagagtca attaactgaa gcgttatttg
     9601 cagaaaaacc agttgctaaa aatgacttga aagaaattcc tcaattagtt actaaaaaga
     9661 atgatgtatc agagtcagag acagttaata tagataataa agacactgtt aaacaaaaag
     9721 aagctaaatt tgaaaatggt gttattacac gtaaagctga tgaaaaaaca actaataata
     9781 cagctgttga caagaaatca ggtaaacaat ctaaaaaaac aacaccttca aataaacgaa
     9841 atgcatcaaa agcatctaca aataaaactt caggtcagaa aaagcaacat aataagaaat
     9901 catcacaagg tgcaaagaaa caaagtagtt caagtaagtc aactcaaaag aataatcaaa
     9961 ctagtaataa gaattcaaaa acaacaaatg ctaaatcatc caatgcatca aaaacgccaa
    10021 atgctaaagt tgaaaaagct aatagtaaaa tagagaaacg tacatttaat gactaagatt
    10081 actaaaatcg tttaataatc aatcatagca gtaagctatg tattatattt ttaaaaattg
    10141 aatttcaaaa taaaaggtag gcattcaagt gcttaccttt tttgatatca tcagtttatt
    10201 ttgaaaggca acgcgatcat tccatgtatt tatgttgttt gtaacatcgt tagattacaa
    10261 tagtgatatt gcacatttta agctgaagat ggaaaaatat tcgataatca aataaacaat
    10321 gaatcttaga aggtacaatg acgttcacta atttaaatat agctgaatgt gttggtgagt
    10381 gatgttcact atagatttat attaatatac aaagacaaag gttgttaatt tttattaagc
    10441 gttaggttga atgtatgaga attttagatt tataatagaa gatagaaacg aaaatttttc
    10501 ttaaaagcag taatgttgac tcaaaataag ctataataat gacacttatt taattgatta
//
LOCUS       DAHHYJ010000094        10411 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000094 DAHHYJ010000000
VERSION     DAHHYJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.094"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(41..874)
                     /locus_tag="NBK22_001833"
     CDS             complement(41..874)
                     /locus_tag="NBK22_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD2415017.1"
                     /translation="MEVKTFYNGNTMPQIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLFFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDETIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(1086..1994)
                     /locus_tag="NBK22_001834"
     CDS             complement(1086..1994)
                     /locus_tag="NBK22_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCD2415018.1"
                     /translation="MDLSSPVVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHLVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(2119..3312)
                     /locus_tag="NBK22_001835"
     CDS             complement(2119..3312)
                     /locus_tag="NBK22_001835"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCD2415019.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(3418..3529)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3684..5276
                     /gene="pckA"
                     /locus_tag="NBK22_001836"
     CDS             3684..5276
                     /gene="pckA"
                     /locus_tag="NBK22_001836"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCD2415020.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGEINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(5654..6400)
                     /locus_tag="NBK22_001837"
     CDS             complement(5654..6400)
                     /locus_tag="NBK22_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCD2415021.1"
                     /translation="MPIESFTHQFEEITYLSDDLQVKALMMTPHHEVNRIVVYLRGGK
                     GQIGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTQLLRLLHDKYP
                     HAFIHMVGFSRGGLQGLLTFQDLPVTSYTIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVELHS"
     gene            complement(6405..6878)
                     /gene="ytkD"
                     /locus_tag="NBK22_001838"
     CDS             complement(6405..6878)
                     /gene="ytkD"
                     /locus_tag="NBK22_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCD2415022.1"
                     /translation="MKFRDKDNRQVNLTFKKDNELADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELAQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            6944..7201
                     /gene="yidD"
                     /locus_tag="NBK22_001839"
     CDS             6944..7201
                     /gene="yidD"
                     /locus_tag="NBK22_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCD2415023.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSASKHSHKHNH"
     gene            complement(7198..8199)
                     /gene="menC"
                     /locus_tag="NBK22_001840"
     CDS             complement(7198..8199)
                     /gene="menC"
                     /locus_tag="NBK22_001840"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCD2415024.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQMFHVLPSFSVAYGATASGLSNKQLESLKATKPTRIKLKWTPQIMHQIRVLR
                     ELDFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDDVVDGTIPPIAL
                     DEKATSLSAIINLIGLYNVKVAVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVTKSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(8204..9682)
                     /gene="menE"
                     /locus_tag="NBK22_001841"
     CDS             complement(8204..9682)
                     /gene="menE"
                     /locus_tag="NBK22_001841"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCD2415025.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNGLLDNTMGIQYDTSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDTVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIVYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(9841..10296)
                     /locus_tag="NBK22_001842"
     CDS             complement(9841..10296)
                     /locus_tag="NBK22_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCD2415026.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYITDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
BASE COUNT         3234 a         2020 c         1589 g         3568 t
ORIGIN      
        1 acaattcatc taaccttttc atcagttgag cgattttaat ctatccttca aaagtttttg
       61 gatcaggtcc aattctctta tcttgattta aaccatcaat tcgcgtcatt tgttcatctg
      121 ataattcgaa atcaaatatt tggaagtttt cagagattct gtttggtgtt accgatttag
      181 ggattgtaac cacaccatgc tgcacattcc atcttaaaac aacttgggca ggtgactttc
      241 ctaattcttg agcaatgtct ttaattgtct catcatttaa aatttgtgca ttcatcaatg
      301 gtgaccaaga ttccatcacg atatgttgtg ctgccaaata taatttcaat ttatgttgcg
      361 ttaaatatgg atgatattca acttgattaa ttacaggttt aattgacact tgtgccaaca
      421 aagcttccaa atgttcaggt tcaaaattgc tgacacctat atttttaact ttattatttt
      481 tatataaatc ttccatacct ttccatgtat caaccattac ggcttcgttc gtacctggcc
      541 aatgtactag atacaaatct aagtatttta aacctaatct agataaacta gcttcgtaag
      601 cagctgctac attttcacga ccgaaatcct caaaaaataa ttttgaagta ataaataagt
      661 cttctctagc aataccagtt gactccaatc cggcacgaat gccagcacct acttgttctt
      721 cattcccata aacttttgcg gtatcaatac tacgatatcc ttgttcaatg gcatacttaa
      781 cactttccat gcaattttca tcattttcca cacgaaatgt ccctaaacca atttgtggca
      841 tcgtgtttcc attataaaat gttttaacct ccataaatat cgcctcacct ttttgatgta
      901 ttataccctg ttatcataac aaatctgagt tgaatacatg agaaaaaaca cttagagcaa
      961 tcaaccacta aaattctagt aatatctctc aaacattaat caaaatgtaa aagcaattct
     1021 gtttaaatta tgacaaacta aaaaagccga agtcacaaca tatagtcatc acttcagcct
     1081 aacatttagt tgaatgattc aattttatcc atcatttgtt gtaagtcttc cacgttgtat
     1141 tgaatacgac catggaatac aaatttgtta aagaactcgt ctaattgttc agcaccgaca
     1201 agcactttga cagcactatt ttgattataa tttgaaatcg ttacatcgcc ttcattttta
     1261 ggattaaagt ataaaattga agttggcgta tatttggcac ctaattcttt ttgtaagtct
     1321 tcagccaatt gtttaatcgc ctcaatttga tctgaataat ttacaaaaga taatgaacgt
     1381 ttgtcatcat tttgatccat cacaatagtt tgtggtctag atttatctaa atccaatgta
     1441 tcaaatactt gttccattgg tggtaaatct ttaaattgac cgccactaat accattataa
     1501 acatgacctt ttaacaattg agaatcaata atataaagac cagttcttgt taatactaaa
     1561 tgactaattc gttcaatatt attaaagcca tcctttggta aaaagatatt tgccataatg
     1621 tgcatatctt ctggtcgaat tcgtttttct ttaactaatc tttcacgaat accaattaat
     1681 ctcatgtccg ttacatattc actatgattt ttcgagaaca attttaatgc gtcaatctca
     1741 cgatcttttg tactaaccat gtgattataa tcttcttgtt gttttgtaat tgtcttttta
     1801 ttttgaatac gctctttctc taaagcttct tcatgagact ttttaatgtt ttgttcttgt
     1861 tgttcatact tttcttctgt ttgtcgctta acttttttct tactacctaa ggcaactaag
     1921 aaaaggacaa aaaagattaa tgcaatgact actgcaataa tgagtccaat gactaccggt
     1981 gaagataaat ccatcacaac aacgctcctt tttaatatat gaataacttt aattataata
     2041 gaaaagctaa agattttcga tacatattat catttatata tcgaaaatct tttatttagc
     2101 tatattcaat tcatcttatt attttactgc gtcttttaat tcttccactt tgtctaattt
     2161 ttcccatggg aataagacat ctgtacgtcc aaaatgacca taagcagcag tttgtttgta
     2221 aattggttgt ttcaaatcaa gcattttaat aataccagca ggtcttaggt caaagtgttt
     2281 tctaactgct tcaacaagtt gcccttcaga aactttacct gttccaaatg tatcaattgc
     2341 aattgacact ggttctgcaa caccaatcgc atatgccaat tgtacttcac attgatctgc
     2401 taaacctgct gcaacaatat ttttagccac ataacgtgca gcgtatgcag ctgaacggtc
     2461 tacttttgta ggatccttac cactgaagca tccgccacca tgacgtgcat agccaccgta
     2521 cgtatcaaca atgattttac gtcctgttaa tcctgcatca ccttgaggtc caccgattac
     2581 aaagcgtcct gtaggattga tgtagaattt agtttgttca ttaatcaagt tttctggaac
     2641 agttggataa atgacatgtg ctttaatgtc ttcttgaatt tgttcaagtg tcacatcctc
     2701 agcatgttgt gttgatacga caatcgtatc aatacgtact gggttatcat tttcatcata
     2761 ttcaacagtg acctgaactt taccgtctgg tcgtaaataa tttaacgtac catctttacg
     2821 cacatctgat aaacgttttg ccaattgatg tgataaataa attgctagag gcatatacgt
     2881 ctctgtttcg ttcgttgcgt aaccaaacat taaaccttgg tcacctgcac ctgttgcttc
     2941 aatttcttct tcgctatctt tatcacgata ctctaatgct ttatccacgc cttgtgcaat
     3001 gtcaggtgat tgttcatcaa tcgcagttaa aattgccatt gtttcataat cataaccata
     3061 ttttgctctt gtgtatccaa tttctttaat tgtttctcta acaactttcg gaatatcaac
     3121 atatgttgtt gtagaaattt cgccggcgat caatgccata cctgttgtaa cagttgtttc
     3181 acaagctaca cgtgcatttg ggtcgtcttt taaaatagca tctaatattg catctgacac
     3241 ttggtcagcg attttatctg ggtgtccttc tgtaacagac tctgaagtaa ataatcgttt
     3301 gttatttaac atagtttgct cctttaaatt tatattacga aaattctctc tctgtgagct
     3361 aaataaaaaa gaccttctaa ctattaatat agagagaagg cctaatacgt ccattcgctc
     3421 ttatcgttca gacctatttg tctgcaaacg gtttggcacc tttcttttat aaaaaaagag
     3481 gttgctgggt ttcattgggt ccatgtccct ccaccactca ggataagaga atccgttaaa
     3541 aataatagta cctaattaat gaattaatgt caatttttca caaataaatt tacagtaaaa
     3601 tattgtagat taattatgtt aatgtgttat actaattaaa tgtaaaggct tacatttaaa
     3661 ttatcgcttt ggagggattt aggatgtcag tagacacata cactgaaaca actaaaattg
     3721 acaaattact gaaaaaacca acgtcacatt ttcaactttc gacgacacaa ctttataata
     3781 aaatcttaga caataacgaa ggggtattaa cagaacttgg tgctgttaat gcaagtactg
     3841 gaaaatatac tggtcgttcg cctaaagaca aattttttgt ctctgaacct tcatatagag
     3901 ataacattga ttggggagaa attaatcaac ctatcgatga agaaactttc ttgaagttat
     3961 accataaagt actagactat ttagataaaa aagatgaact atacgtattt aaaggctacg
     4021 ctggtagcga taaagataca atgttaaaac ttacagtcat caatgaatta gcatggcata
     4081 atttatttgc taaaaatatg tttattagac ctgaatcaaa agaagaagct acaaagatta
     4141 aacctaactt cactatcgtt tctgcaccac attttaaagc agatccagaa gttgatggta
     4201 ctaaatctga aacctttgtc attatttcat ttaaacacaa agtcatttta atcggcggta
     4261 ctgaatacgc tggtgaaatg aaaaaaggta tcttctctgt aatgaattat ctcttaccga
     4321 tgcaagatat tatgagcatg cattgctcag caaacgttgg tgaaaaaggc gatgttgcat
     4381 tattctttgg tctatctggc actggtaaaa caaccttatc ggctgaccca caccgtaaac
     4441 taattggtga tgatgaacac ggctggaata aaaacggggt ctttaatatc gaaggtggct
     4501 gctatgcaaa agcaattaat ctttccaaag aaaaagaacc acagattttt gacgcaatca
     4561 aatatggtgc tattttagag aacactgtag ttgcagaaga tggttcagtg gactttgaag
     4621 acaatcgtta tacagaaaac acgcgtgccg cttatccaat taatcacatt gacaatattg
     4681 tagtaccatc aaaagcagca catccaaata caattatttt cttaactgcg gatgcatttg
     4741 gtgttattcc accgatttca aagttaaata aagaccaagc aatgtatcat ttcttgagtg
     4801 gtttcacttc taaattagct ggtacagagc gtggtgtgac agaacctgaa ccatcattct
     4861 caacatgttt cggagcaccg ttcttcccgt tacaccctac tgtttacgct gatctattag
     4921 gtgaacttat cgatttacat gatgttgatg tttatcttgt taatactgga tggactggcg
     4981 gaaaatatgg tgtaggacgt agaatcagct tacattacac acgtcaaatg gtaaaccaag
     5041 cgatttctgg caaattgaaa aatgcagaat atacaaaaga tagtacgttt ggtttaagca
     5101 ttcctgtaga aattgaagat gtaccgaaaa caattttaaa tccaattaat gcttggagcg
     5161 acaaagagaa atataaagca caagcagaag atttaattca acgttttgaa aagaacttcg
     5221 aaaaatttgg tgaaaaagtt gaacatattg ctgaaaaagg tagcttcaac aaataaattt
     5281 gaatactaat taaagagcca accgttgtta tagtctaaca atggttggct cctcttattt
     5341 tatatggtaa aaatttatag gcaattttat tacaacaatg cacatttaag gtgaccttca
     5401 tgccaaactc gcatcactca ttcaatggaa gcaacacgtc ttcacataaa gtaccgattc
     5461 cttacgctat gcatgttgcc ccattttgca taaagtactt atcgttaact caacgcaagt
     5521 tgttcctatt cacaaaaagc acatacggtt atctgcatgg agcacatttt cattgaaagc
     5581 gcatacccct cattcaacgt atatcacaca tcttcaaagc ttgatagttc ccatgcgcac
     5641 accacgtttc atactagcta tgcaactcaa cttggttcat aaactcttta atataagtca
     5701 atgtttcaac catcgctggt ggtcttggca catgtccttc tgccatttga taaaatgttt
     5761 catgcgtggc accttttaac tctagttggt ccgctaaata atacgcatga tgaataccaa
     5821 cttgctggtc tttccctcca tgtacaatta atattggcgg actgttttca ttaatgtttg
     5881 gaatcgcttg gcgtgcctca tatgccgctc gatctttttt cggatgacca atcattcttc
     5941 gtagcattcc tcttaaatcg acacgttctt catacattaa atcaatatct gagacaccac
     6001 cccagattgt ataacttgtt actggtaagt cttgaaatgt caacaatcct tgtaaaccac
     6061 ctcgcgaaaa accaaccatg tggataaatg catgtggata tttatcatgt agcaatctta
     6121 atagttgcgt cacatcattt aaatcgccac gataaaattc atctttacct tcactgccat
     6181 tgttacctcg gtagtatggc ccaatcacta aagtttgact atctgaaaat tgcattaatc
     6241 tacctgcgcg cacacgtcct atttgacctt tgccacctcg caaatatact acaatgcgat
     6301 ttacttcatg atgtggtgtc atcattaaag cttttacttg taagtcatct gacaaatatg
     6361 taatttcttc gaattgatgc gtaaaagatt caattggcat tcgtttacgt ttgataaaat
     6421 ccaagtgatt gcaccctctc tacgcatttt aaaatggtac tatcttgcag taagaaactc
     6481 cgttgtgcga gttcaatatc attgatacag ttaaacaaca ctggccctgc tgtttctaaa
     6541 taatcgttct tgcttaccaa tgattcaact tcgataaaat atacatcttt tacaaaatca
     6601 gtttgatcat gtgtttcaat ggtatattgt gctatgtaat gaatattttt aactttggcg
     6661 cctgtttctt catataattc acgggtaact gcttcagcac tactttcccc gcgttccctt
     6721 ttaccaccag gaaattcaat gccccgtaaa ttatgtttgg taaaaagcaa ttgattttta
     6781 aacgttggaa tagctagcac atgattgcca tctgctagct cattatcctt tttaaatgtc
     6841 aaattaactt gacgattatc tttatcccta aacttcacgc gcatcacatc cctacattgt
     6901 atgttaatat aatagttaat tactattgtt ggaggcattt attatgaaaa agatattctt
     6961 ggcgatgatt catttttatc aacgtttcat ttcgccactc actccaccaa cttgtcgttt
     7021 ttatccaaca tgttcagagt acactagaga agcgattcaa taccacggtg ctttcaaagg
     7081 cctttattta ggtatccgtc gtattttaaa atgtcatccg cttcataaag gtggctttga
     7141 ccctgttccg ttaaaaaaag acaagtcagc aagcaagcat tcacataaac ataatcatta
     7201 atatggttgt aattgagtta tatccactaa aggggggcga aattcgagtc gcccctcttt
     7261 taatatgcct gatttagtaa ccacatcttg ttcaaaataa taacctgctg gtgtaacatc
     7321 tcctggataa tcacctttac gagcaagcat cgctgtaaaa tagcggctta aaccatattc
     7381 gtacatgccg ccaataacca cttttacacc atgacttttc aaagtatcaa ttgccgtttg
     7441 cactttatca atgccaccta gacgaaacgg ttttaataca gcaactttca cattgtataa
     7501 tcctatcaaa ttaattatag ccgacagtga cgtcgccttt tcatcaagag ctattggagg
     7561 tattgttcca tccactacgt catcaagcat tgagatatct ttaaatggct cttcgatata
     7621 aagaacctgt tcacgcgcta atagctgtaa ctgtgtgaaa tcttgacgat ccaatgactc
     7681 atttgcatct ataaccaatt gaaagtgaaa gtctaattcc cgtaacactc taatttgatg
     7741 catgatttga ggcgtccatt ttaatttaat tctggtcggc tttgttgctt tcaatgactc
     7801 tagttgttta tttgataagc cgctcgctgt cgctccatat gctactgaaa atgaaggcag
     7861 tacatgaaac atttgataca atgccatgac aatagttgcc cttgcagcag gcgtattttc
     7921 caatgaatct actaatttta gtgctgcttc atacgtttcg aatgatttat ttctattatc
     7981 ttcgaaccat tgctcaatta catgtttcac tgaggcaatt gtttcatgat cataccaatc
     8041 tgtttgaaaa gcgttacatt ccccgaaata tgcatttcct ttgtcatcaa tcaattcgat
     8101 aaacaaacaa tcacgatgcg ttaaagtgac tttcggcgtt acaatttgtg acttaaatgg
     8161 ctcactatat ttataaaaat gcaaagctgt caacttcatc aaatcatcct ctatacaact
     8221 tatttctttg taatttacct gttgatgtat aaggtaaagt atcaaccttt tcaaagtgtt
     8281 tcggtacttt atatttcgct aaatgttgtg ataaatatac aatcaattgt gcctttgaaa
     8341 tgtcactttc actgacaaaa tataatttag gcacttggcc ccaagtatca tcaggatgcc
     8401 ctacacatac tgtgtcactg atacctggaa attgcttcgc taccgtttca atttgatatg
     8461 gataaatatt ttcaccgcca ctaataatta aatctttacg tcggtcataa atcatgacat
     8521 aaccttcatg atctatttca gcaatgtcac ccgtattaaa ataaccattt tcaaacgtac
     8581 ccgttaaatc tgttggatac aaatatccat tcatcacatt ggcgccttta atcattaatt
     8641 ctccatgacc ttctttatta ggatttttaa tttttacgtc aacattggca cttggcatcc
     8701 ctacagtgtc aggacgtgca tgcaacattt ccggtgttgc tgttaaaaat tgcgaacatg
     8761 tctcagtcat accaaatgaa ttataaattg gcaggttata ttgtaatgcc gtctctatca
     8821 tagtggcaga taatttagca ccgccgagta atattttttg taaattataa ggttcatgta
     8881 aaccttgttg cataagccaa tttaaagttt gtggcacaag cgaaatgtgc gtgatgcgtt
     8941 catttttaat catcgttaaa atttgttcgg cattgaattt atcaacaatg cgcacagtaa
     9001 aaccttcaat aacagctctt aaaagtacac tgagacccga aatatgataa atcggcaaga
     9061 cagatagcca attagtgtca cgatcaaatc ccaagctctc tttacatccg attgcactgg
     9121 cataatgatt acgaaacgtt tgtggcaccg ctttttgagg gcccgttgtc cctgatgtaa
     9181 acataatcga tgcaatgtca tctaaattaa atgatgtatt taatatgttg gacggcgact
     9241 ctttcggcac cacagtttca ttcgatgtat catattggat acccattgtg ttgtccaaca
     9301 aaccgttagt tgtaatatcc cttccagcga attcaatatc atccagcgat acaatttgaa
     9361 accctcgtaa ttccagtggc aaggtacaaa aaatcaattg tacatcgatt gacttcatct
     9421 gattcgtcat ctcattaggt gtcaaccttg tattaatcat cgcaatttca atatttgcca
     9481 accaacatgc atgtattaaa atgatcgatt gaatcgaatt atctatgtat agcccaacac
     9541 gagattgttg ataagccttg agtcttttag ccaatagact cgcttcacag tataaatttt
     9601 gataagtata agattcttga ccgtctgtta tcgcaatatg atgtccattt tgttgtgctt
     9661 gtttatataa ccaaaagtcc atgcgttatt cctccaaaat catttacatt ataattataa
     9721 cgattttatg acattctagc agtggttatg tttaaaaata taaaaaagta gacgaattga
     9781 tgcattgata tgattgttat aatgctcaat acatatcgtt atatcattcg tctactatta
     9841 tcagttattt ttatttaatt ttagtgtcat tctgtcattt tgatgtggtg atttacccat
     9901 tgttgccaca tcatctgcaa tgtcaattgg tatacggttc atgtcttgta atgcacttaa
     9961 atggaatact tcatcatcta aattttcaat gagatataca taatatgtta ccttgtcctt
    10021 tttatatttt aacgttttcc aaaagtccgg cttgcaattc aatacattat ccgtaatata
    10081 ttcaataaat aagtaacgtt tgctgcctac tttgtctatg aaatattttg cagtgccttt
    10141 ttctatacct cttatatgtg catagtctgc tgaaaagtaa atactaccta ttgtttcatt
    10201 atgttgttgt atttcaaatc gttggcctac tattttatta tttgtgccac aactacttaa
    10261 aaaaatcagt aaatatagca ttaaacatat tttcatcccc ttgaatttta aaaacttttt
    10321 caaaagcaac acctctaaaa ataaatacaa tatattatag caaatagaaa ttattatttt
    10381 gttaaattat gttgtcatgt tcgtgtcatt t
//
LOCUS       DAHHYJ010000095        10346 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000095 DAHHYJ010000000
VERSION     DAHHYJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.095"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            128..766
                     /locus_tag="NBK22_001843"
     CDS             128..766
                     /locus_tag="NBK22_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCD2415027.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            769..1692
                     /locus_tag="NBK22_001844"
     CDS             769..1692
                     /locus_tag="NBK22_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD2415028.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            1704..2972
                     /locus_tag="NBK22_001845"
     CDS             1704..2972
                     /locus_tag="NBK22_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD2415029.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            2972..3595
                     /gene="udk"
                     /locus_tag="NBK22_001846"
     CDS             2972..3595
                     /gene="udk"
                     /locus_tag="NBK22_001846"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCD2415030.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            3623..4099
                     /gene="greA"
                     /locus_tag="NBK22_001847"
     CDS             3623..4099
                     /gene="greA"
                     /locus_tag="NBK22_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCD2415031.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            4425..5159
                     /gene="pxpB"
                     /locus_tag="NBK22_001848"
     CDS             4425..5159
                     /gene="pxpB"
                     /locus_tag="NBK22_001848"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCD2415032.1"
                     /translation="MDVRFINEQTIMIYFENKISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDEQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     KFIQIERDISDGNFNVDDWVVIENVN"
     gene            5149..6159
                     /locus_tag="NBK22_001849"
     CDS             5149..6159
                     /locus_tag="NBK22_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCD2415033.1"
                     /translation="MSIKILQPGLFSTVQDIGRKGYEHIGFSGAGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSINASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNHPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            6161..6610
                     /locus_tag="NBK22_001850"
     CDS             6161..6610
                     /locus_tag="NBK22_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCD2415034.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEIDFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVNVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            6624..7985
                     /locus_tag="NBK22_001851"
     CDS             6624..7985
                     /locus_tag="NBK22_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD2415035.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAEGDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIENLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKVTLDEMVIDGFSTTADFLYVVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            7985..8737
                     /gene="pxpA"
                     /locus_tag="NBK22_001852"
     CDS             7985..8737
                     /gene="pxpA"
                     /locus_tag="NBK22_001852"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCD2415036.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIANVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            8750..9979
                     /locus_tag="NBK22_001853"
     CDS             8750..9979
                     /locus_tag="NBK22_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCD2415037.1"
                     /translation="MGRNLKLKKESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQTA
                     VFTSQFFASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISILI
                     AFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMIL
                     GIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAHR
                     ILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVFE
                     HAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLNERSNLIVIVFIVISTM
                     IFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAVL
                     VTILTGIFSFKEVLQLF"
BASE COUNT         3699 a         1418 c         2035 g         3194 t
ORIGIN      
        1 aatagctttg cgtctaacac gaaatcagtc atttaattgg ttggcaaaat tataatcaat
       61 tgtgaaggct gatttatttt attgtcataa tcatattgac ttattttaca taaaggaagt
      121 ttaaaacatg gatgacctaa ataaaaaata tttaatagat ttacatcaac atcaaaatag
      181 ttcaatcgaa gttttgcgtg aatttgccga agtaaatgaa gtgccaattg tagatcgttt
      241 aacattagat ttaattaagc aattaattcg tatgaataat gttaaaaata ttttagaaat
      301 tggtacagca atcggctata gttctatgca attcgcttct atatctgatg acattcatgt
      361 cacaacgata gagcgtaatg aaacgatgat tcaatatgct aaacaaaatt tagctactta
      421 tcattttgaa aaccaggttc gaattattga aggtaatgct ttagagcaat ttgaaaatgt
      481 aaatgacaaa gtttatgata tgatatttat tgatgcagca aaagcgcaat caaagaaatt
      541 ttttgaaata tatacaccac ttttaaagca ccaaggtctc gtaattacag ataatgtttt
      601 atatcacggt tttgtatcgg atattgggat tgttagatcg agaaatgtaa gacaaatggt
      661 taaaaaggtt caagattata atgagtggtt aataaagcaa ccaggatata caacgaattt
      721 tttaaatata gacgatggat tagcgatttc aattaaagga gaatgactat gacagaatta
      781 cttgtcacac ccaagtcatt aagccatatg gaaacactta ttgatttagg tgcagatgct
      841 tttgtaattg gcgagcaaaa gtttggttta agacttccag gggaattcaa tagacaacaa
      901 atgacagagg ctgtcgcatt agctcacaaa aacgataaaa aagtatatgc ggcggtgaat
      961 ggattatttc ataattatca tttagatgct gtagaagatt atattaattt tttacatgaa
     1021 atacgagttg acagaattat tttcggggat ccagcagtag taatgtatgt gaaggcgcaa
     1081 gagaatccaa taccacttca ttggaatgca gagacgttag taactaatca ttttcaatgt
     1141 aattactggg gtaaaagagg cgcatcaaga gctgtcttag cacgtgagct gaatttggat
     1201 gaaattatta atataaaaga aaattcaaat gtggaaattg aggttcaagt tcaaggaatg
     1261 acttgtatgt tccaatcaaa gcgtatgcta ctaggcaatt attatacgtt ccaagatcgt
     1321 caaatgaaaa ttgaacgtcg caatgatgaa caatctttat tactttatga tgaagaaaga
     1381 caaaataatt acccagttta cgaagattac aatggtacgc acattatgtc accaaacgac
     1441 atttgtttaa ttgaagaatt agcaccattt tttgaggcgg gtatagattc atttaaaatc
     1501 gatggtattc tacaaacgga agaatatatt aatgtggtaa cggaacagta tcgtcaagcg
     1561 atagatttgt acaatgaaga tcctgaaatc tatgaagatg agaaatttat gttgatggac
     1621 ccaattgaag aaattcaacc tgatcatcga ccatttgacg aaggtttctt atataaacaa
     1681 acagtatatt aaggaggtta atcatgaaga caatagaaga gattaaatca actcctaaaa
     1741 cagttatgaa gaaaccagaa ttattagcac ctgctggaaa cttagaaaag ttaaaaatag
     1801 cagtacatta tggcgctgat gccgtatttt taggtggtca agaatatgga ttacgttcaa
     1861 atgctgataa tttcacgatg gaagaaatag ctgaaggtgt tgaatttgcg aaccgttacg
     1921 gtgccaaaat ttatgttacg acaaatatta ttgcacatga tgagaatatt gaaggtctag
     1981 aatcatattt gcgtaatttg gaaaagactg gtgcgacagg tatcattgtt gcagatcctt
     2041 taattatcga aacatgtaaa gaagttgcgc caaaacttga aattcattta tctactcaac
     2101 aatcactttc taattacaaa gctgtagaat attggaaaga agaaggattg gatcgtgttg
     2161 tattagcacg tgagaccggc gcgatggaaa tgcgtgaaat gaaggaaaaa gtagatattg
     2221 aaatcgaagc atttattcat ggtgctatgt gtatcgccta ttcaggtaga tgtacattaa
     2281 gtaatcatat gactgcaagg gattccaaca gaggcggttg ctgtcaaagt tgccgttggg
     2341 attatgaatt attagaagtt gatgataatg gtgaacttga tgttttttat aatcaaggtg
     2401 aagttacacc gtttgcgatg agtcctaaag atttaaaatt aatcgaatca attcctcaaa
     2461 tgatggatat tggtgtggac tcattaaaaa ttgaaggacg tatgaagtca attcattata
     2521 ttgcaacagt tgtctcagta tatcgtaaag tcattgatgc gtatgcggca gatcctgaca
     2581 actttaagat taatccggaa tggttaattg agttagataa atgtgcaaat agagacactg
     2641 caccagcatt ctttgaggga acacctggtt atgaagaaca gatgtttggt caacaacaat
     2701 ctaaaaaatc accatttgat ttttgtggtt tggtactaga ttataatgaa gatacaaaaa
     2761 ttgcgactat tcaacaacga aataacttta aaccaggtca agaaatagaa ttctttggtc
     2821 cagaaattga aacatttaca caagtagtag aagcaattta tgatgaagaa ggtaacagct
     2881 tagacgcggc gcgccatcca ttacaaatcg ttcaaattaa agtggatcgc ccgatatatc
     2941 caaacaacat gatgagaaag gaaattggct aatgaaagct actacaatca ttggcatagc
     3001 tggtggatct ggctcaggaa aaacaactgt aactaacgaa attatgaaaa acttagaagg
     3061 tcatagtgtc gctttacttg ctcaagatta ctattataaa gatcaaaagc acttgacttt
     3121 cgacgagcgc ctagaaacca attatgacca tccatttgca ttcgataatg atttattaat
     3181 tgaaaatctt aaagacttga aaaatggtaa agcagtagaa gtaccgacat atgattatgc
     3241 tagtcataca agaagtgaca ttaccattga ttttaaacct aaagatgtta ttatcgtaga
     3301 aggtattttc gctttagaaa ataaggtatt acgtgatatg atggatgtta aaatatatgt
     3361 tgatacagat gcagacttga gaatattacg ccgtttaaca cgagatacta aagagcgtgg
     3421 gcgttcaatg gactctgtta tcaatcaata tttaagtgtt gttagaccta tgcatgacca
     3481 atttattgaa ccgactaaga aatatgctga tataattatt cctgaaggtg gaagcaataa
     3541 agttgcaata gatattatga caacaaaaat tcagtcttta gttagcaagc aatagtataa
     3601 ttcagtaaag gaagatgaca atatggaaaa tcaaaagcaa tatccaatga ctcaagaagg
     3661 ttttgaaaaa ttagagcgtg aacttgaaga attaaaaaca gttaagcgtc ctgaagttgt
     3721 agagaaaatt aaagttgcac gttcatttgg tgacttatca gagaactctg agtatgatgc
     3781 agcaaaagat gaacaaggat tcatcgaaca agatattcaa agaattgagc atatgttaag
     3841 aaatgcatta atcattgaag atactggaga taacaacgtt gttaaaattg gtaaaacagt
     3901 aacgtttgta gaattaccag gtgatgaaga ggaaagttat caaatcgttg gttcagctga
     3961 atcagatgca tttaatggta agatttcaaa tgaatcacca atggctaaag cgttaattgg
     4021 taaaggttta gatgatgaag ttcgtgttcc actacctaat ggtggcgaaa tgaacgtaaa
     4081 aattgttaat atccaataaa tgatcaaata gattgtttaa ttgcgcgatt gaagtgtgtt
     4141 aatgatactt cggtcgtgct tttttatatt caaaatcaaa aaatcggaca gatgaaaagt
     4201 aaaaattttc catcagaccg aatgatagca gaacctaaat tagtgtaaga gttacaatat
     4261 taactactcc attttaaaaa agcaaaatat ttccgagaaa acaaattgat tatcatttgt
     4321 tattgtatgc gatttgaatt acttattaaa caacttgtgc ttctcaatga tacaatgaaa
     4381 aggtattcaa tactggttta ttgcaataca aagggggatt taaagtggat gtaagattta
     4441 ttaacgagca gacaattatg atttattttg aaaataaaat atccgaagag acatatcgta
     4501 atgtcacagc aatggtacgg tggatacgag aaaaagaaat attagaaatt caagatattg
     4561 tcccttcgta tcgagcagtt ctaatatatt ttgatgaaca agctataaca tcttctaaat
     4621 taattgaaaa tttagaactt aacaaattta atgaaaaaaa tgttcatgct gtgaatcaaa
     4681 ccaatcgtat tattaaaata cctgttcaat atggtggtac atatggacca gatattgaag
     4741 aagtagcaaa gcataatcga ataactgttg agcaagttat tgaaaaacat acaagtaaac
     4801 cttatttaat atatatgcta ggatttatgc caggatttcc atacttaggc ggacttgatg
     4861 aacaactaca tacacctaga cgtaatcagc caaggttaaa aattcatgca ggttctgtag
     4921 gtattgcgaa taatcaaaca ggactatatc catcagattc tcctggcgga tggcaaatta
     4981 ttggacgcac accgttaaaa gtatttagca gcgagcgtga accaatgtca atgtacgaag
     5041 caggagaatg gatacaattt tatgcaatag atgaacagaa atttatacaa atcgaaagag
     5101 atatatcaga tggcaacttt aatgttgatg actgggtggt gattgaaaat gtcaattaag
     5161 attttacaac caggtctctt ttcaacggta caagatatag gaagaaaagg ttatgaacat
     5221 attggatttt caggcgcagg tgccatggat caatttagtt ttaaggttgc gcagtcatta
     5281 attaacaatg atggtccagc gattgaatat actttgattg gtcctaccat tcaatttaat
     5341 acgcaaaata catttgttat aaccggtggt agtattaatg cttcgctaaa taataaaact
     5401 atatcaatga attctgtcat attagctgag aaaggtgaca ttttaaaaat aggtgctata
     5461 accaaaggtg cacgcggtta tcttactttt ggtcattcta tcaacgtacc ttctattgct
     5521 gaaagttatg caacacatac ccgaagcagt ataggaggat tcaaaggtag aaagttatta
     5581 gctaatgatg taataacagt gaaaataaat aatgacttta aagaaaacat tggaaagact
     5641 attcatttac aggacgattt attgccagaa aataatatta tacacattct tcaaggacct
     5701 caattcgagg cattttctga agaggctaga gcgaaaattg taaatcatcc atatttaatt
     5761 actgaacaat cagaccgtat gggctatcgt ttggaaggtg acagcgttgc accaattaat
     5821 caagcagata tcatttctga accggttgct cttggtagtg ttcaagtacc aaatgatggt
     5881 caacctatta ttctgcttaa tgataaacaa acgataggtg gttataccaa aattgcaact
     5941 gtatgcaaat ttgatctgcc aaagttagca cagatgaaac cacaagatac aattcaattt
     6001 aaatggatat cattccaaga agctgtagat aagaatcggg aacaaatgtc tctgtttaat
     6061 gaaattctta agagtcatca aaagactcca atttttgata catctagctt gcgacatact
     6121 tcgaaaaaat tagcaacaat tttaaaaggg gatttgtaaa atgaatattg aaaaaatcga
     6181 acaaataatc aaattagtga aggaaaatga tgttaagaaa tttaaatata aaaattttga
     6241 agatgaaata gaaattgact tcactgactc gaatcatttg gctgcacaca gtaatcaatc
     6301 aaatcaaagt atgaacaata atgatttgac agcttcaaaa gcgaatgata actccgatgt
     6361 ttcgacaaat gattatcatg acattaaatc accaatggta ggtacattct ttttacaaga
     6421 tagtaaagaa ttaactgaac caattgtgaa tgtcggtgac aaagttaaca agggagatat
     6481 tataggatat gttgaagcga tgaaagtatt aaacgaggta acaacagatg ttgctggaga
     6541 aattactgaa atagtagctg atcatggcac aaatgttgaa tacgaccaag ttttggtacg
     6601 tattaagtaa agggggtaag taaatgcttc gttgtttaat tgcgaacaga ggtgaaattg
     6661 ctgtaaggat tataagagct tgcagagaat atgggattga aactgtagca gtttatgcag
     6721 aaggggacga gcaaagcttg catgtacatt tagcagatca agctatatgt attggggaag
     6781 ctaatgcttt agatagttat ttaaatattg accgcatcat atctgctgca caaatcactg
     6841 gtgctaatgc aattcaccca ggatatggct ttttatcaga atcaacaaaa tttgctcaaa
     6901 ccgttgagga acaaggcata gcgtttatag ggcctactaa aaaaacgatg gaaatgatgg
     6961 gggataaaat aacagcaaga caaacagttc atcatgcagg tgttcctgtg attccaggat
     7021 caaatggtgc tgtgaatcat gtgtctgaaa ttgagaatct tgccaaagac atcggttatc
     7081 cagttgttat caaagctgcc agtggcggtg gtggtaaagg tatacgtatt gtaaagaaag
     7141 ctgaagattt agaaaaggca tttaaagaag ctaaaagtga aggaaaaaaa tactttgatg
     7201 atgatcgtgt ttatgttgag gcttttatac ctgttgcaaa acatgttgaa gttcaagtta
     7261 tgggagatgg gcaggataat tatgtacatt taggggaacg tgattgttct gtacaacgta
     7321 aaaatcaaaa attaatcgaa gaatctcctt gtgcagcatt aactgaagaa agacgacaac
     7381 aaatatgtaa cgatgctgtt aaagttgcac gtgcagcaaa ttatagaagt gcaggtacga
     7441 ttgaattttt agtaactgat accgcacatt actttattga aatgaatgct cgaatacaag
     7501 ttgaacatac tgttacagag atgcgagcag aacgtgattt ggttgcagca caactgtatt
     7561 tattggaaca taatcattta cctttttcac aaagcgatat tcaatttaat ggtcatgtga
     7621 tagaagcgag aataaatgcc gaaaatcctg aaaagaaatt tcaaccaaca ccaggtaaag
     7681 tgacagcgtt acatttacct caaggcttta atgtgcgagt cgattcttta ctatatcacg
     7741 gttatcaagt ttcaccatat tatgattctt tagtagctaa agtaattgta aaatctcatg
     7801 atagagcttc agcaattgat aagttgaaag tcaccttaga tgaaatggtg atagatggat
     7861 tttctacaac tgccgatttt ttatatgtag ttttaaatta tccactatat cgtgatggag
     7921 atgcaaaaga tgttgatatt aaatttttag aaaaacatca aattgttaaa ggggtggaat
     7981 tataatgcga gttgatttga attgtgattt aggcgaagca tttggaaatt attcctttgg
     8041 tggtgatcat caaattattc cgttaattac aagtgcgaat gttgcttgtg gttttcacgc
     8101 tggtgatgaa aatgtaatga atgaaacggt aaaacttgcc aaagcacata atgttgcagt
     8161 aggtgcacat cctggtttac ctgatttgaa aggctttggc agacgaaata tagatatctc
     8221 taacgacgag atttataatt tgatgattta tcaattaggt gcattacaag ggttttgtcg
     8281 cattcatcaa gttaaaatta atcatgttaa accgcatggt gcattgtatc agatgggtgc
     8341 aaaagacaga gaaatagcaa acgttatagc acaagctgtt tatgactttg atccatcact
     8401 agtgttagta ggattagcaa attcatatct aatttcagaa gcaaagaatg tcggattaat
     8461 tacagcttct gaagtgtttg ctgatagacg atacgaagat gatgggcagc tcgttagtag
     8521 aaaagaaagt gatgctgtga ttactgatac tgacgaagca cttaagcagg ttttaaagat
     8581 ggtgaaggaa aataaagtta tttcaaaaaa caataaggaa gtaacgttac aagcagatac
     8641 aatttgtgtg catggtgatg gagaacatgc attattattt gtttcgaaaa ttagagaaat
     8701 tttaatgaaa gaaggcattg atattcaatc cttatagggg gctaaaaaaa tggggagaaa
     8761 tttaaagctt aaaaaagaaa gtgactttga attcacaaaa aatcataaaa ggttattatt
     8821 aggttctgta tttttgatgg caacttctgc aattgggcca gcatttttaa cgcaaacagc
     8881 agtatttaca tcacaatttt ttgcaagttt cgcatttgcc atattactgt ctatcatcat
     8941 tgacattggt gcacaaatta atatatggcg catattagtt gtaactggtt taagaggtca
     9001 agaaatatca aataaagttg ttcctgggct tggtactgtt atctcaatat tgattgcatt
     9061 tggtggtcta gcttttaata ttggtaatat tgctggtgcc ggtttaggtt taaatgcaat
     9121 ttttggatta gatgtaaaat ggggcgcagc tattactgca atctttgcaa tattaatctt
     9181 tgtaagtaaa agtggccaaa aaattatgga cgttgtttca atgattcttg gtattgtgat
     9241 gattttagtt gtggcatatg tgatgtttgt ttctaatcca ccttatggtg atgcttttgt
     9301 gcatacattt gcgccagaac atccaatgaa attagtcttg cccatcatta cgttagttgg
     9361 tggaactgta ggtggttata ttacctttgc aggtgcacat cgtatattag actctggcat
     9421 taaaggtaag caatatttac catttgtaaa tcaatcggca attgctggta ttttaactac
     9481 aggtattatg agaacgttac tattcctagc ggtattagga gttgttgtaa caggtgtgac
     9541 actaagttct gaaaatccac cagcgtcagt ttttgaacac gcaattggac caattggaaa
     9601 gaatattttt ggtattgtgt tatttgctgc agctatgtca tcagtaattg gctcagcata
     9661 cacaagcgca acatttttaa aaacacttca taaatcactt aacgaaagaa gtaatttaat
     9721 tgtgattgtg tttatcgtta tttcaacaat gattttctta tttattggaa aaccaatcag
     9781 ccttttaatt atagcaggcg cgataaatgg atggatttta cctattacgt taggtgcaat
     9841 tttgattgca agtaaaaaga aatcaattgt cggtgattat aaacacccaa attggatgtt
     9901 catctttggt atcgttgcag tacttgttac aatattgacg ggtatattct cattcaaaga
     9961 agtacttcag ttattttaaa agacattttg actaatagca tcactgtaaa tgttgttagt
    10021 attaggcaac atgtattttg atttccggtg attcatttat aaagcataat tagatatatt
    10081 aagagagagt ggtgcatgac aaatgtactg ctctttttgt tgtgtatcag tattgtgatg
    10141 ttttaatgcg aatattatat gaatcattat gaaatttaat aaaaataaaa gaaatgatta
    10201 tcattttttc tgatatactg ttaaacggtt tggaattttt atgtttacac tgtattggtt
    10261 gatataactc aactaataat tacgaacagg gtatttcaaa ttggaaagta ttatgaacgt
    10321 gatacataat caaaattgta ggctca
//
LOCUS       DAHHYJ010000096         9983 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000096 DAHHYJ010000000
VERSION     DAHHYJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.096"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(471..1025)
                     /locus_tag="NBK22_001854"
     CDS             complement(471..1025)
                     /locus_tag="NBK22_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCD2415038.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TLDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(1346..2746)
                     /locus_tag="NBK22_001855"
     CDS             complement(1346..2746)
                     /locus_tag="NBK22_001855"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCD2415039.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLLKKPTQMQDGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTTVDDIITSQKQYKIVYSNQQEDVF
                     DGVLVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(2770..3693)
                     /gene="hemH"
                     /locus_tag="NBK22_001856"
     CDS             complement(2770..3693)
                     /gene="hemH"
                     /locus_tag="NBK22_001856"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCD2415040.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTPVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(3751..4788)
                     /gene="hemE"
                     /locus_tag="NBK22_001857"
     CDS             complement(3751..4788)
                     /gene="hemE"
                     /locus_tag="NBK22_001857"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCD2415041.1"
                     /translation="MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(4778..4888)
                     /locus_tag="NBK22_001858"
     CDS             complement(4778..4888)
                     /locus_tag="NBK22_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415042.1"
                     /translation="MIKCQFYDIYLLKIRTNERYDNIDKNDFNLGGLYGA"
     gene            5051..5554
                     /gene="traP"
                     /locus_tag="NBK22_001859"
     CDS             5051..5554
                     /gene="traP"
                     /locus_tag="NBK22_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCD2415043.1"
                     /translation="MKKLYTSYGTYGFLHQIKINNPTHQLFQFSASDTSVIFEETDGE
                     TVLKSPSIYEVIKEIGEFSEHHFYCAIFIPSTEDHAYQLEKKLISVDDNFRNFGGFKS
                     YRLLRPAKGTTYKIYFGFADRHAYEDFKQSDAFNDHFSKDALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(5678..6901)
                     /locus_tag="NBK22_001860"
     CDS             complement(5678..6901)
                     /locus_tag="NBK22_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415044.1"
                     /translation="MHNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFSLIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRLRWQWYQS
                     GLKTWQVNLISFITFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMVWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVTFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(6894..7634)
                     /locus_tag="NBK22_001861"
     CDS             complement(6894..7634)
                     /locus_tag="NBK22_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2415045.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHKQTLDDIYIHVTQGGDVHA"
     gene            7768..8190
                     /locus_tag="NBK22_001862"
     CDS             7768..8190
                     /locus_tag="NBK22_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCD2415046.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            8332..8697
                     /locus_tag="NBK22_001863"
     CDS             8332..8697
                     /locus_tag="NBK22_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD2415047.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDFNPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            9430..>9983
                     /locus_tag="NBK22_001864"
     CDS             9430..>9983
                     /locus_tag="NBK22_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCD2415048.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     NYGQYIEEHSTGINILKKIKNPYH"
BASE COUNT         3284 a         1804 c         1376 g         3519 t
ORIGIN      
        1 taatattatt ttgaattacc taattaatat accataatca aaaacctaaa gtcaagaact
       61 ttttgaattt aatttaaatg ttatctctta aataattact taaatatcgt agcaacatgt
      121 tctctgttga acacaaatat tagtatattc atttttgtag tgtacgtcaa cgacattttc
      181 aaagtttttt gtgtaaaaaa acgcttctta ttccctttta tcatataagt gtctaatagt
      241 tgtcataaat agtgttaaag catttaaaaa ggtataggag ttataaagtt tacaacgcct
      301 ataccttctg aaaaagaaat tataaaagct tgttacaccg catattcttt cagtcagcga
      361 ctaccaatat aacattgtag ccctaagaca ttgcttgacg cctcaattac aacaattttt
      421 caaaatcagc agctacctac tgacacaaca taacacaacc catacactaa ctatcgtgtc
      481 atgtaatctt gcatccgatc ttgcaacgct gtaaatgttt cgaagccatc ctcttctaag
      541 aagtgccctc catcttccac gattcgcaag ttcccctcta atgcattcat taaacgctgg
      601 gtttctttat aagaaacgta tttgtcattt ttagaactca atccgtaaaa attgtcaact
      661 ttctttttaa tattatcgta atcaatggtt acattactta aatcaatatc taaatctata
      721 ttttctgcat cttctttaaa gcccgctata ctgaaaaagc cttcaatcgg ctgatcaatc
      781 atttcaatat attttaaagc tgtgattgaa cctaaaccat gtgttacaaa atatgtatcc
      841 tttttgcgta cattaatttg tttcgtcata gcttcaatcc actgatccag tgtcttcgct
      901 tcaggtgatt caaaattaaa taatgttacg tcatatcctt ctaaagttaa gttatgctcc
      961 aaccactgat accaatgatt tctactattt ccatgcatag aatgtacaat aattacatct
     1021 gtcatctcat tctctccttt caacttacta cttcttttct atttaaaaaa atgaatgatt
     1081 acctataatt gtaaaataaa aacaccttaa ttagaaatgt tttatcgcaa agtgacattt
     1141 ctaattaaag tgcattgtca tcatttcaat atcattcaat aacagctaaa cctttgtctc
     1201 tgcttcaatt tcacaaaaat aattcccgct gaaagtatct atatttacac attacttcca
     1261 ccattatata acttaaaaat gactatattt catcaaacat tatctaaagg cgtcgcacct
     1321 acaccaacac catccaacaa ttaacttaca actctgcgat tacttcttca gcagcaactt
     1381 taccttgcgt aatacaatca ggtagtccaa ccgcttcaaa agatgcacca gttactctaa
     1441 gtcgtggata tgtttgttta atatgtgctt gaatctgtct aatttgttga atatgaccga
     1501 catggtactg tggcatactt ttcggcaaac gattgacaat tgtaaattca ggatcacctt
     1561 taaatgtcat catttgactt aaatctctac gtacaatcga tactaattca ttatctgtat
     1621 gatcatcaac cacagtatca cctggtttac ctacatacgc acgaatcaaa accttacctt
     1681 ctggtgtagt aaatggccat tttttcgatg tccaagtaca tgcggtaatg tctgtatcac
     1741 tcgttctcgc aatcacgaag ccagtaccat cataagtatt ttcaatatct ttttcatcaa
     1801 atgccaatac aacagttgca acagtcgtac tatccatcgt tttaaagtaa tcaaatgctg
     1861 gatcttgccc gaaccaattc aaaaagactt gatgtggtgt tgtcactaat accccatcga
     1921 atacatcttc ttgttgatta ctgtaaacaa ttttatattg cttttgagat gtaataatat
     1981 catccactgt cgtattgtag cgtattgtca cacctttatt tttcacatct tgttctaatg
     2041 cttcaataaa tgaacttaaa ccatgcttaa attgtttgaa ttgtcctttt ggtgcgccag
     2101 gatataattg tctttgtttc agacgcttat ttttctcatc cttcatacct tttatcagac
     2161 ttccgaatgc ctcttctttt tctttaaaat taggaaacgt actcatcaaa cttaatttat
     2221 caatatcggt accataaata ccacccatta aaggctctat taaattctca agtacctcat
     2281 tacctaatct tgctctgaaa aatgccccaa cagaaatgtc accatcctgc atttgagtag
     2341 gttttttaag taaatctaac cctgctctta actttccaag tggtgaaatt aatttagttg
     2401 tcacaaacgg tttgatatct gtcggaattc ccataattga tccacctgga ataggataca
     2461 atttgttttt cgcaaaaata taagattgtc cagtcgtatt tgtaacaata tcttgttcta
     2521 atccaatatc tttcgctaat tctgtcatga tcgtttttct acctaaataa gattcaggcc
     2581 ctagttcaat catataacca tctttacgat acgattgaat ctttcccccc ggacgattcg
     2641 atgcttcaaa gatggttaca tcaatattag gatcttgctg ttttaaaaaa tatgcacttg
     2701 ataaacctgt tatccccgct cctataatag ccactgattt agtcacaacg tttcacgctt
     2761 ctttcgtcat taaaatatag acttgatttc atcaacaatt gcaccgataa ataatggatg
     2821 tgtattcggc atttttggac gataataatt cgcaccaata tcatcgcaaa caactttaca
     2881 ttcataatca ttgtcataaa gcacctctaa atgctcacat acaaaaccta ctggcgtata
     2941 tataaagttt ttatactgat gtttttcata taaatcacgt gttaaatctt gtacatctgg
     3001 ccctaaccaa ggtgtacctg tattaccttc agattgccaa ccaatcgcaa tatgttcaat
     3061 attagattgt tctttaatta aaagtgcagt atgttctagt tcttgtggat atggatcatt
     3121 attcttttcg attaaacctt ttggcaaact atgagccgaa acaactaata ccgtgtcttt
     3181 atgttcctct tccggtattt gagctaatgt ttcgttgact ttattcgtcc aatattcaat
     3241 aaatttaggt tgttcataat aatgtttcac atgtgtaagt tgaataccat attttgcagc
     3301 ttcttcatca gcacgtttgt catatgatcc tactgaaaat gaagaataat gtggtgctag
     3361 tactacagta attgcttcag taatgccatc attgtgcatt tgttcaaccg catcttcgat
     3421 aaatggtgaa atgtgtttta atcctaagta tagtttaaat tcaacatctg catatgcttt
     3481 atttaatgct gaaactagcg catcagcttg gtcatctgtt gtacctgcta atggtgataa
     3541 accacctata aattcatatc tatctttcaa atcttgaagt tcttcttcag atggacgttt
     3601 accatgtcta atatctgtat aatatggctc tatgtcactt tctttataag gtgtgccata
     3661 agccataact aataatccca tttttttagt cattgataat accttccttt aaatgaatta
     3721 tctttcatgt gcttcaatgt aatactatga ttatctttgt gtatatgtgt gtacgaattc
     3781 gcttacttta cgtaacgtct ctggttgcac ttctgggaaa acaccgtgtc ctaaattaaa
     3841 gatgtgttta ccgttctcca taccttgatc taatattggt ttcaatctct cttcaatgac
     3901 attccatggt gctaataaaa ttgatggatc taaattccct tgtaatgttt tagtaacgcc
     3961 taattgttga gcctgattaa tagacgttct ccaatctagg cctaatacat caatcggtaa
     4021 atcattccat tcattgatta aatgactggc acctacaccg aataaaatta ccggcacatc
     4081 atgtttttct ttaacctcac tgattaatcg aatcatatgt ggtttaatgt aacgtctgta
     4141 atcctcgaca tttaatgcac ctacccatga atcgaaaatt tgaatcaatt cggcgcctgc
     4201 ttcgacttga gctgttacat atttaacaga tacatcaact aaatgattca ttaaagcaaa
     4261 ccatgttgct tcatctctat acatcatcgc ttttgtaaaa ttgtaatttt tcgatggtcc
     4321 gccttcaatc atatatgacg ctaatgtaaa tggtgcccca gtaaatccta ttagcggcac
     4381 atttaacttt tcttctgtta aaagtttaat tgtatctaat acatatggta catctcgttc
     4441 ggggtctatt tgagaaagtt tctcaacatc ttgaattgtt ttgataggat tatgaatcac
     4501 tggaccaata cccgatttaa tttctacatc gacaccaatt ggctttaatg gtgtcataat
     4561 atctttgtat aaaattgctg catctgtatg ataattatca actggtaaat gtgttacata
     4621 agcgcacaac tccggctgat gtgtaatatc gaatagtgaa tatttttctt tcaattttcg
     4681 atattctggt tgcgaacggc cagcttgtcg cataaaccaa acaggtgtat gtgatgtttc
     4741 ttcacctttg atcattttta aaattgtatt gtttttatta tgcaccataa aggcctccta
     4801 aattaaaatc attcttatct atattatcat atcgctcatt cgttcgtatt ttcaataaat
     4861 aaatgtcata aaactgacat ttaatcatag aactatttat tgtaaattta aattctaaag
     4921 tccattattt tgtatcatta cttctaaata tctcgcaaga ttcattatag taattttaat
     4981 caattattaa tagtggtaat gactagttta tcatcgtata ataaataaaa acataagggg
     5041 gacctttcat atgaagaaac tatatacatc ttatggcact tatggatttt tacatcagat
     5101 aaaaatcaat aacccgaccc atcaactatt ccaattttca gcatcagata cttcagttat
     5161 ttttgaagaa actgatggtg agactgtttt aaaatcacct tcaatatatg aagttattaa
     5221 agaaattggt gaattcagtg aacatcattt ctattgtgca atcttcattc catcaacaga
     5281 agatcatgca tatcaacttg aaaagaaact gattagtgta gacgataatt tcagaaactt
     5341 tggtggcttt aaaagctatc gtttgttaag acctgctaaa ggtacaacat acaaaattta
     5401 tttcggattt gctgatcgac atgcatacga agactttaag caatctgatg cctttaatga
     5461 ccatttttca aaagacgcat taagtcatta ctttggttca agcggacaac attcaagtta
     5521 ttttgaaaga tatctatacc caataaaaga atagattcga agaaaacttc cattggttat
     5581 gctgttaacg tatagatgct aatgtaatct gaacgccgta tcatttggcg ttcaacatta
     5641 catgcacctt tacaacatac atgccaatgg aagtttttta gtctcgtaat aatgtttcct
     5701 gatattttaa ctttttaata atacttctaa tcgttaataa acctacaata tagaaaatga
     5761 gtacaacata gaataacgtc atatgtttta taataaatgt cactgcaaac acagtacaaa
     5821 taacaaacat taatctatat aaaaactgtt catacccttt gattaccttc tcttcaggta
     5881 caggccacac ttgaggccat aagccatatg cttgctgtga ataaaattgt gccatttgta
     5941 acaatataat atatacaaat aaacacccaa taattgctgt cactaatgga tatgataacc
     6001 aaaccattaa taaaactgca ataattacta acctaaagat aatattaaat gcgtctctcc
     6061 ctcttataaa gcttctaata aataagaata aatacatcgc attagagtta aatttactac
     6121 cctttggaac tggtaaaagt atatctagat aacttcttct gactgcagat tctttcaaat
     6181 gttttacatc ggtgaacata ttaacaaatt tataataatt catatgatgt cgatgttcga
     6241 ttgcaatcat tttctcccaa ggatacaaaa agcctggttt atatttttta actaaaaatt
     6301 ctattaacac aggcaaagca accatcacaa atgcgatgta ccattttgga gctaatagta
     6361 agtaatatgt tagagcaaag gtgatgaatg atattaaatt aacttgccat gttttaagtc
     6421 ccgattgata ccattgccat cttaagcgta aaccaacata tggaaaaatt aatgcactga
     6481 ctccaaaaca aatataaaat gccacattat gttgattaat attgtaaaac aacgggaaca
     6541 ttacaataac aataatgagt tggattaata tgcgcgcaaa gtaactatat aaaatcgcat
     6601 gacgcataaa ttgagacatg tgtttttcaa atggtaataa aaagatttta tccgcttctt
     6661 ttaggagtgg tcgcattgga aaaatggatg tcaatgctac aatcactgct gctattaatg
     6721 aaaaattgat attcgttgga atatgtttta accattcacc atatccaaaa ataaatgcac
     6781 ccagcaaaat aagtaaaaag accatgaaat gaccattaaa tataaactta ttataataat
     6841 ttttctcttt acgaagggca tgtaatcttt tattaaataa tgtggtcgct tggttatgca
     6901 tgtacatctc caccttgcgt cacatgaata tatatatcgt ctaatgtttg tttatgtaag
     6961 ccagtttgtt gtctcaatgc ttctaaatct ccaaatgcaa cgacttcacc ttcgtctagt
     7021 atgataaaac gatcacaata acgttcagct gttgctaaaa tatgtgtact cattagaacg
     7081 gttctacctt cgtttttctt ttcaaccatt aaatctaaca tggattgaat tcctaatgga
     7141 tctaggccaa ggaatggttc gtctataata tacaattcgg gattaacgat aaacgcacaa
     7201 ataatcatga ctttttgttt catcccctta gaaaaatgac tcggaaaaac tttcaattca
     7261 ttttctaaac ggaatgtctt taataatggc attgctcgat tcatcgcttc atcacgatca
     7321 atatcatatg ccattgctgt catctcaatg tgttcctcta atgtgagttc ttcataaata
     7381 accggtgatt ccggaatata agataacttt cttctataag cctctatgtc atcattaatg
     7441 ttgatatctg aaattgatag agatccttcc ataggtgtaa gcaatcctag catatgttta
     7501 atcgttgtac tcttaccagc gccattaagg ccaataagtc caacaatttc gcctttgttt
     7561 aattcaaaat ttatatcttt aattacaggg cgttttccat atccacctgt aagctgttct
     7621 actttaactg tcataaggca cctccatgac ttatattgta ccaaaaatta taaaatgctc
     7681 atattaaata cacatgtcct aatatcgaat ttttagcgac aatgttataa tgaatggtaa
     7741 tacaagttga aaaggagtgt agtcatcatg tcagaaacaa ttttcggcaa aattttaact
     7801 ggagaaattc ctagctttaa agtatatgaa gacgattatg tctatgcctt tttagatata
     7861 tcacaagtta ctaaaggaca tacgttatta attcctaaaa aagcttctgc taatatcttt
     7921 gaaactgatg aagaaacaat gaaacatatc ggtgcagcat tacctaaagt agcaaatgct
     7981 attaagcgtg catttaatcc tgatggttta aacattattc aaaataatgg tgagtttgca
     8041 gatcaatctg tatttcatat tcatttccac ttaattcctc gatatgaaaa tgatattgat
     8101 ggatttggtt ataagtggga aacacatgaa gacattttag ataacgatgc aaaacaacaa
     8161 attgctgaac aaattcaagc acaattttaa atgtatgctt aatctaagct cgaacgggta
     8221 taatatgatt aatattataa caattgcgtt tgaagtgata acatcaaggt tagcaatttt
     8281 aaacaaaatg agttatcaag ataacagatg ttaaaagtga ggagaatata aatgaaagca
     8341 tcacgcattc tattcggtat cggtgttggt gtagcagctg gttttgtagt tgcacttcaa
     8401 ggacgagacg acaaaagtgt caagaacaac acgatcgatc gtactgcccc tactggttca
     8461 aaatcagaac tacaacgtga atttgaaacg attaaacaaa gttttaatga cattttaaac
     8521 tatggtgttc aaattaaaaa cgaaagtgcg gaatttggta gttcaattgg tggtgaaatt
     8581 aagtcattac ttggaaactt caaatctgac ttcaatccta atattgaacg tttacagtca
     8641 cacatcgaaa atttacaaaa tcgtggcgag gatattggaa acgaaatttc taagtagcag
     8701 gttacgttct cgatcacaac tatttttatt agtaactgca tatttatttt ttaaatttaa
     8761 atgccaaata aacgagatga cattagaaat tagatatttc ttgtcatctc ttttttaaaa
     8821 ctcaaatgaa cttatgttta caaattatag gaagacattg tttgtagtga ttttcgctta
     8881 aatcctattt atgaattgat cgaaaacatt gcttaggatt cattatgtta tccttgcact
     8941 ttgattacgc tttacttaaa tcattatcga caaacaacat acttatattt tcattgagcc
     9001 gaaccttata tacacattac atatacctta cttgcacaaa ttattaatct ggtgtttatt
     9061 ataattacat atcactatat ttttagcatt tgtataactt agttggtcaa aagatgcttt
     9121 tgcatatgcc ctgccatatc cattttttat attattcaag ttttcaaata ctcatgtcac
     9181 acgcgccttt ttcataagtg atgctttatt agcaagaata tgtgttcgca gaaatttgtt
     9241 ctgcatccta cttctacgct agtcaatcag acaattttac caatccccac tttcgcgttt
     9301 caaatcaaac aatacgtcgc ccatttcttc ttatataaca attcttctaa catgatatgt
     9361 tactattgaa ttactgaacc tgagttagtt ataatctaac ttatattgaa aagagatgag
     9421 gcgtaagata tgtttttatg taaaagacaa attgatatca atgcacgatt tggtttgcct
     9481 agaattgcat ttatgagtgc agttgcaacc atcattatgt ttttagttag ttatgaagta
     9541 atgtattttt tatctaatac gccattatca gatagacatt ttctcatctt tttattactt
     9601 gtatttatga cgtatccatt acataaaagt atacatttat tatttttctt accatataga
     9661 aaatcgttta aagttcataa gttaactaaa agaaaatggc ttatattcta taatacctac
     9721 gtcaatcaac ctgtacacaa attttatttt tgcattaact taatattgcc gttaattatc
     9781 ttatctgcaa tgttcgttta tctaacaatt tcattcccgc aatatggaca ttattttatg
     9841 ttcttattgg cattgaattt cggtatttcc attacagatt tattatattt aaaaataatt
     9901 atattttcta attatggaca atatatagaa gaacatagta caggtattaa tattttgaaa
     9961 aaaattaaaa atccatatca ttt
//
LOCUS       DAHHYJ010000097         9919 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000097 DAHHYJ010000000
VERSION     DAHHYJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.097"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..927
                     /locus_tag="NBK22_001865"
     CDS             <1..927
                     /locus_tag="NBK22_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCD2415049.1"
                     /translation="SNKVNRSEQVNKSNNHAENPKPKEGVGTWVGKDIKVLTSKFGQA
                     DRVYPFRDGYKNYVFKDKNSYYIVSTKREEIVSVYATGEKVNVSPLKIGQHSAEIFNH
                     TSINPEPSFKVDGKKYEFELSDEDLKTQTLIKYGDIYAQVYSDQQSKKVLSVRFLTKE
                     MLADIEPYQLNSNSTSEEHNKRPVEQNPNQLISLYEVTNEMRKLKGLKPLKINSDLAH
                     IASNNLYEATSNGSDSVEFTEDALRGQLDKNHVTYKTTAQNVGYAFNDVPTLIHSWMN
                     SDIHRSRLLNSKYDEMGGDVMRDYYSLIFLEK"
     gene            943..1377
                     /locus_tag="NBK22_001866"
     CDS             943..1377
                     /locus_tag="NBK22_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD2415050.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCATGGSCNCSL"
     gene            complement(1440..2366)
                     /locus_tag="NBK22_001867"
     CDS             complement(1440..2366)
                     /locus_tag="NBK22_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD2415051.1"
                     /translation="MKTVNRNILNAVLVGAGFLSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIVQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNIIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQTIPNK"
     gene            2605..2859
                     /locus_tag="NBK22_001868"
     CDS             2605..2859
                     /locus_tag="NBK22_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCD2415052.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            complement(2862..3251)
                     /locus_tag="NBK22_001869"
     CDS             complement(2862..3251)
                     /locus_tag="NBK22_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415053.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            3321..3863
                     /gene="rsmD"
                     /locus_tag="NBK22_001870"
     CDS             3321..3863
                     /gene="rsmD"
                     /locus_tag="NBK22_001870"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCD2415054.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            3865..4347
                     /gene="coaD"
                     /locus_tag="NBK22_001871"
     CDS             3865..4347
                     /gene="coaD"
                     /locus_tag="NBK22_001871"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCD2415055.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(4409..5548)
                     /locus_tag="NBK22_001872"
     CDS             complement(4409..5548)
                     /locus_tag="NBK22_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCD2415056.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALASPNNILGISY
                     LKAIAKNAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTDSLKNIYTVTEGFENRLKSNIYEATDFHHFV
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTRNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            5675..6232
                     /locus_tag="NBK22_001873"
     CDS             5675..6232
                     /locus_tag="NBK22_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCD2415057.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            6312..6485
                     /gene="rpmF"
                     /locus_tag="NBK22_001874"
     CDS             6312..6485
                     /gene="rpmF"
                     /locus_tag="NBK22_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCD2415058.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(6639..8573)
                     /gene="isdB"
                     /locus_tag="NBK22_001875"
     CDS             complement(6639..8573)
                     /gene="isdB"
                     /locus_tag="NBK22_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCD2415059.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAEET
                     GGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEVK
                     APKETKEVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKKDGT
                     QQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKD
                     YAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEK
                     LLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNV
                     QPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWK
                     DFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDK
                     EAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPSV
                     EKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQ
                     TSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPL
                     MALLALSSIVAFVLPRKRKN"
     gene            complement(8776..9828)
                     /gene="isdA"
                     /locus_tag="NBK22_001876"
     CDS             complement(8776..9828)
                     /gene="isdA"
                     /locus_tag="NBK22_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCD2415060.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQTKTQTAHTVKTAQTAQEQNKVQTPVKDVAT
                     TKSESNNQAVSDNKSQQTNKVTKQKETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
BASE COUNT         3115 a         1396 c         1766 g         3642 t
ORIGIN      
        1 agtaataaag taaatagatc tgaacaggtg aataaatcaa ataaccatgc tgaaaatcca
       61 aagcctaaag aaggtgttgg tacatgggta ggtaaagata ttaaagtgct tacttctaaa
      121 tttggacaag cagatcgtgt ctaccctttt agagatggtt acaaaaatta tgtgtttaaa
      181 gacaaaaaca gttattacat tgtttcaact aaacgtgaag agatcgtttc agtgtatgct
      241 acaggtgaga aagtcaatgt tagtccgtta aaaataggac aacattctgc agaaattttt
      301 aatcatacaa gtattaatcc agaaccgtcc tttaaagttg atggtaaaaa atatgaattt
      361 gaactttcag atgaagattt aaaaacacaa acactgatta aatatggcga catatatgca
      421 caagtgtatt ctgatcaaca atcgaaaaag gtgctaagtg tacgattttt aacaaaagaa
      481 atgttagcag atattgaacc ttatcaatta aattctaatt ctacgtcaga agagcataat
      541 aagcgtccag ttgagcaaaa tccaaatcaa ttaatttctc tttatgaagt aacgaatgaa
      601 atgagaaaat taaaaggatt aaaaccattg aaaatcaata gcgatttagc acatattgca
      661 tctaataact tatatgaagc gacctctaat ggttctgata gtgttgaatt tacagaggac
      721 gcattaagag ggcaattaga taaaaatcac gttacttata aaacaactgc tcaaaatgtt
      781 ggttatgcgt ttaatgatgt accaacatta atccatagtt ggatgaattc agatatacat
      841 cgatctcgtc tattaaattc aaaatacgat gagatgggtg gagatgtaat gagagattat
      901 tattcactaa ttttcttaga aaaataacaa tgaggggtat ttatgattaa tgaagcttca
      961 ctagcgatat tagatgatat tgatgaacta gctgatatga tagttgcatc agatatttat
     1021 gcatcatttg agcaagctaa gcaggcgctc gaaaacaatg acgaagcaca tttactttat
     1081 caatcatttt taaaatcaaa agagaaatat gatgaagtaa tgcgtttcgg gaaatatcat
     1141 cctgattata agaaagtcat gttagagaca agacaacgca aaagagcata tgagatgctt
     1201 gacgttgtga tgcattacaa agctagggaa atggcacttc aacatttaat tgatgaagtt
     1261 gttacgaaaa ttgcgtacgc tgtttcagag catgtcaaaa tagaaacagg taatccattc
     1321 tttcaaacat cacatagtgg ttgtgcgacg ggcggatcct gtaattgttc attataaaaa
     1381 acatcgagtc agaaaaagat ggttattgaa accactaact agcatctgac tcgatgtttt
     1441 tatttgttcg ggattgtttg tttgaattgt tgtgctaaat ctggtcgatc tgtcacaatc
     1501 gtgtgtgcac ctttttggta taaatcattc atcagatcta tactatttac gccataataa
     1561 cctggaatga tattcatatc atttaaccat ttgataaaac gagatgaagt caaatcaatg
     1621 cctttaaaat gagtaggcat ttggaacgtt tgtgctaatg gttggtagta cctaccacct
     1681 aataaatgat attttaaaaa tgcttctgta acttcctgtt ggctagcacc aattgcgacg
     1741 gatccttgta caattttatt aaaacgaacg atttgttctt tataaaaact tgtcacaaga
     1801 acgcggtcaa atgcttgatt ttctgcaatt gtatcaaaca taatttgtgg tgcgattgag
     1861 ccttcatagg attcaggagc atcttttaaa tctacgttta tatacatatc aggatattgc
     1921 ttcagcaact catcgaaggt tagtatagct gtgtgtgcat gaccacgata tggtgttagt
     1981 ccattgatat ctttgaagtg ataagctgcg tctaatttct ttaattctgc taatgtatgg
     2041 gcactaactt ttccagagcc gttcgtcgtt ctatcaacag ttgcgtcatg aaaaacgata
     2101 agctgttgat cttttgtgag tctcacatct gtttcaaagc catcaacgcc taattgttta
     2161 gcatagtcaa atgcaagttg cgtttgctct ggtcttaaag ccataccacc gcgatgcgca
     2221 aatatatatg gtgcattgcc tttgaaaaaa gcagggatgg tttgcttttt agtaatcact
     2281 ttatttttat tgatcattaa tagactactt aaaaatccag caccgactag taccgcattt
     2341 aaaatgtttc tgtttactgt tttcatagaa aattcctcct cgtttacttt tatagcttaa
     2401 caaaatcata gttctgacta cagcgaaatg tgtggttaat catatagtga tgctcttgag
     2461 aattgaatta atataatgat gtttaatata atctaaatac atttatcgag caattacata
     2521 agacataatt aaggtagctt aatattttaa aattaataaa gacatgatat gattaatgct
     2581 gaaaataaat ggagtgaggt ttaaatgaat ttaatcccaa gaactagtat tgtagtttat
     2641 ttaaaacata tgaaacatga acgacaaatc cgaaaatatg gacatatcgt tcattcaaat
     2701 agagatcgta aatttgtaat tatgtatgtg aatgagcagg atgttgatca aattgtacat
     2761 aaactaatgc aacttaaata cgttagacat attgatggct caccatataa atacttaaag
     2821 aaaacttacg aaaaagagaa acacgaaata tataattaat attattgaag ttctggtatc
     2881 catttttgga gccttaacac actaattaaa taattataat ataacgataa atcatgaaat
     2941 gcttgagggg gctgtacatc gatacctaaa ataggtatgt cgaatttttc agcacaatca
     3001 ttaatcattt gaaacttttt atttgaatct ggatatggat atttaatagc gtttatcatg
     3061 atataaaatg cttggaaatg attgccagtt gtagggttca taatgattgc tacagatgac
     3121 tttttatttt cagcttgtgg tgaatgaaaa tagataattt tatcaatacg ttgatggtta
     3181 tattcaatca tcgtcatgaa ttcgaacaaa tctgttagac cttcaccaag tgtaataaat
     3241 gtttgtttca ttggtttatc attctccttt ttctcaataa attatattaa ataactaaat
     3301 gaattggaag tgtgaacaac atgcgcgtca ttgcaggtaa acataaaagt aaagctttag
     3361 aaagtatgga aggccgtaat acgagaccaa ctatggataa agttaaagaa ggtatcttta
     3421 atagtttata tgatgtgtca ggtataggtt tagatttatt tgcaggaagc ggggcgcttg
     3481 gaatagaagc actctctcga ggtatggata aggtaatctt tgttgatcaa aattttaaag
     3541 ctgtaaaagt tattaaatca aatcttgcga atttggattt agaggcacaa tctgaagttt
     3601 ataaaaataa tgcagataga gctttaaaag cattgtcaaa acgtgatatt caatttgatg
     3661 tcattttctt agatccacct tataataaag gtctcattga taaagcttta aaactaattt
     3721 cagagtttaa tttattgaaa gaaaatggta tcatcgtttg tgaatttagc aatcatgaag
     3781 aaatagacta tcaaccgttt aatatgatta aacgttacca ttatgggttg acagacacat
     3841 tgttattaga aaagggagaa tagcatggaa catacaatag cggtcattcc gggtagtttt
     3901 gaccccatta cttatggtca tttagacatt attgagagaa gtacagatag atttgatgaa
     3961 attcatgtct gtgttcttaa aaatagtaaa aaagaaggta cgtttagttt agaagagcgt
     4021 atggatttaa ttgaacaatc tgttaaacat ttacctaatg tcaaggttca tcaatttagt
     4081 ggtttactag tcgattattg tgaacaagta ggagctaaaa caatcatacg tggtttaaga
     4141 gcagtcagtg attttgaata tgaattacgc ttaacttcaa tgaataaaaa gttgaacaat
     4201 gaaattgaaa cgttatatat gatgtctagt actaattatt catttataag ttcaagtatt
     4261 gttaaagaag ttgcagctta tcgagcagat atttctgaat tcgttccacc ttatgttgaa
     4321 aaggcattga agaagaaatt taagtaataa aaataacagt attttaggtt tatcatggtt
     4381 tacaatccta aaatactgtt ttcatttgtt aacgatattg ctgtatgaca ggcgtgttga
     4441 aatctgtttg ttgttgcccg cttattgcat tgtatatgtg tgttgctttg atttcatttg
     4501 tgaagtaatg tgcattgctt ttgttaatat tggttatata ttgtctttct gggaacgctg
     4561 tttttaaatg ctttaaatat tgtctgccac ggtcgttcat cgctaatact ttaactgcgt
     4621 gaatgtttct cgtaacatct gtaggtttaa tgtttaataa tacattcatt aacagtcttt
     4681 ggatatgcgt atatgtataa cgctttgttt ttagtaattt tacaaaatga tgaaaatcag
     4741 ttgcttcata aatgttagat ttcaaacgat tttcaaaacc ttcagtaaca gtataaatat
     4801 tttttaatga atctgtagtc atagctatga tttgatattt caaatatgga aatatttgat
     4861 ttaatgttat atgaggtgtt acgtacaagt gttgaatatc tttaggtacc acatgatgcc
     4921 aatgatcatc ttgactaatg attgatgttc taatagatgt accacttgca aactgatggt
     4981 gttgaattaa tgaatcatga tgttgagcat tttctcgttt gatagaaatt gcattgatgt
     5041 ttttagcatt tttagcaatt gctttcaggt aactaatacc aagtatgttg ttaggacttg
     5101 ctagtgcttc atgatgctct aataattcgc taatgatacg agggtagctt ttaccttctt
     5161 ttactttttg tgaaaaggat tcagattgtt caatttcatt aatgctgtgt gctaattgct
     5221 ttaatgtttt gatatcatta ttttcactac caaatgcaat tgtatcgaca ctcatataat
     5281 ctgcgacttt aactgctagt tcggcaaaat gatcgccaga tgataaactg gcagttgctg
     5341 gtagttcgat aactaaatca gctgttgata atgccatttt tgcacgagta aacttattat
     5401 agattgctgg ttcgccacgc atgacaaagt taccactcat tattgcaata gtaacgtcag
     5461 catttgtaag ttttttagat tgattaatat gatattgatg cccattatga aagggattat
     5521 attctgtgat taagccaacg cttttcattt gcaattcgtc ctttcggtat tcattattaa
     5581 tattgtaaca gaacatgata tgttaagaaa aaatcttgac aacttgttct tagaaagtta
     5641 aaataaattt tgtgcttgtt agaggtgaag ccatatgaaa tggtcaatta cgcaattaag
     5701 gaaatatcaa ggtaagccat ttgaatttga tcaaacggtg agttttgaca atttaaaaga
     5761 atcattagat ttaattgatt tatctccaat tacaatccaa ggtcagttaa ccattaagtc
     5821 aacagaagtc gttgcggata ttcacattac tggaacgtat acaatgcctt gtgcacgtac
     5881 tcttgtacca gttaaagtcc cactagatgt aactactaca gaagtatttg atttagaagg
     5941 gtacaatcag tataacgatg atcaagatga tgtagatgaa cactatcaca ttattaaaga
     6001 tggtatggtt aatcttcagg atattgtcga ggatatagtt attattgaga aaccaatgag
     6061 agcttattca gagcaaagtg accaaatgtt gacagtaggt aatggttggg aagtaatcga
     6121 tgaggatcaa ttagatgagc ttgctaaaca gcaagaacaa gatgattcag aatcacgaca
     6181 agttgatcca aggcttcaaa aattacaaca attatatgat aaagagcaat aagtagttta
     6241 attaatgata taatgttgtt aattaaacta acatttagta tttttcgttt ataatctaag
     6301 gaggatatat catggcagta ccaaaaagaa gaacttctaa aactagaaaa aacaaacgtc
     6361 gtacgcattt caaaatttca gtaccaggta tgactgaatg cccaaactgt ggcgaataca
     6421 aattatcaca ccgtgtatgt aaaaactgtg gttcttacaa tggcgaagaa gtagcagcta
     6481 aataatttta gttactcaat ataaaaagtc ccgcttaaaa tgattgtttt aagtgggact
     6541 ttttatattg agaaaaataa ttggcgaacg aggtaactgg atacctcatc cgccaattaa
     6601 aatttgttaa tttaataatt aaatataaag acgatttatt agtttttacg ttttctaggt
     6661 aatacgaatg caacgatgct acttaaagct aataatgcca ttaatggtaa tgtcatatct
     6721 ttatttgatt cttcaccagt ttgtggtaat gattttgctt tattttcttg tgtattttta
     6781 ttgttttggc tttgagtgtg tccatcattt gtgtttttaa tgtttgcttt ttgtaatgga
     6841 gcactatctt ttgcttcgct agaacctgct gaagtttgaa caacatcttt tgttgttttt
     6901 gatgaagcag ttgttggttt tgcaacattt tgagtcgtag atactacctt agttggagtt
     6961 gtactacttg attctacttc acctttagtt ggttttgtag caggcgtttt gtctttacct
     7021 gactcactag atgcgtcatt ttctttttca acacttggta attgtttatt gtcatctttt
     7081 tggctgtctt gtttttgtga ttctttttca acaggtgatg gtgttggttt gctaggcgta
     7141 gctggagtag cttccttctt agctgagtta tcttgttgtt cttttttgtt agatttatcg
     7201 gtattggctt ttgtaaatgc ttctttatca acgattctga catggtattg tccatcataa
     7261 tcaatcgttt ttacgtgaac tttaacgata gcatcatata gagttttacc ttcaacatat
     7321 gggaaaataa ttgttctagt attattttta gcatctttgc ttatagttct aacacgttga
     7381 ccttcaacca tgaaatcttt ccagtaatca tcattagtag tttccatgac catatatttt
     7441 ttgccgttaa gcatacctgt tttaataggg tgtttaacaa aagtatccat catagattcg
     7501 ttattctcaa cactttcata aacaacatat tttgtatctt gtaaatcagt cattttttca
     7561 tttgttggtt gtacattttg gaattcagta atagctgatt tcacttgctc atctaaagct
     7621 ttctttgtat cctctaattt cttcttgtac tcagccttta atttttcagg aagtttatct
     7681 tgaattttat ttaattcata aacttgtctt tctagtgttt tcgctttttt atatggcgct
     7741 aataattttt cagctttata atcttcttca gttttgaatt tatctgcact gttataaatt
     7801 ggttgtgcga attccattaa tgtgtaatcg tatttttctt ctttgttatt gaagtgtgtt
     7861 gaactaacaa ttttaacagc ttttgttcca tttgatacag agaagcgaat gtaagcataa
     7921 tctttaacag tatcgtatga tactaattta attggcaact ttttgtcacc ttcataaact
     7981 tcaaattttc tccaaaattg acctgattgt aatcctaatt caatttctgg ttttgaatca
     8041 gtgaaaataa ctctagcagg tttaacagaa cttgcataat gataaaactg ttgagttcca
     8101 tctttctttt tcatttcaaa atcaattgga cgagagtttg gtgcgctatg atctttgtct
     8161 tttattgcag ggtttttaat cgcttctcta agttcctgat tcaaaatagg atatgtattg
     8221 ttagtggctt ttgctgctgg tttaacttct tttgtttcct taggggcttt aacttcttta
     8281 acttctttag cttcttttgt ttcagaagta ggggcctcaa cttctttatt agatactgag
     8341 acagcattag ctactggttt agtttctgga gctttttcag atgttgttgt tggacttgca
     8401 actgcttcag tttttggttg tgcttctgta tttgtaccac ctgtttcttc agctgcttgt
     8461 gcttcgccat ttgacattaa taataaaagt gtactaattg ctacagatgc aacgcctagt
     8521 gatgactttc taattgaata aaatgattta aattcttttt gctgtttgtt catgttgtag
     8581 aaacaactcc taattgtata ttatcaactg ataatcatta tcaattgatt acttgtattg
     8641 tacattgaaa tggtagttaa ttgcaacgtt attttataaa aaaataacct atgttataga
     8701 ttatgaaata tctatgacat aggttaaata aaatttaaaa taaaaaatat attaagttat
     8761 gagtgtgacg atgatttatt tagattcttt tcttttgaaa agtaataaag ataatgaacc
     8821 taaaagggca agtgttgcaa aggcaactgt gctaataaag ttatcaactg aagttaaacc
     8881 agtttttggt aattctttag ctttagatgc ttgtttaggc gtttcctttt gttttgtaac
     8941 tttgttagtt tgttgtgatt tattatcact tacagcttga ttgttgcttt cagatttcgt
     9001 tgttgcaaca tctttaacag gtgtttgaac tttattttgt tcttgagcag tttgtgctgt
     9061 tttaactgta tgagcagttt gtgttttagt ttgatctttt gttgtatcag tacttacaac
     9121 tttagtagag tgattgtctt caactttgct tgttgtagtt acagtaggtt taactttttc
     9181 aactttaggt tgaactggtt tagtttgctc agtaggtgtt ttaggttgag ctggttttgg
     9241 ttgaaccggt ttaacattgt ttggttttgc tgcgtcagct aatgtaggaa ttgctttttc
     9301 aaattccaaa tgcgtagtat atctatgatt gtaattaatt tgtggcacga caatatgtac
     9361 tttagtagtt aagctcttat atccaggttc aactgcaaca ttgattgttc tagtatccgc
     9421 ttttttatta tcgttaacaa cagttgttgc taattcttga ttgtttgcat tgtaaaattt
     9481 gtattctttc cagaatgatg cattgtttaa cacggtttgg aaataatatt tattattttg
     9541 tttaattact ttaccagggt gttgcatata gtcatccatg tgtgacttct ctgaagagcc
     9601 atctttttgc acttggaaat taattggttg tgatgttgct tgagaaactt gtgtgctttg
     9661 attattagtt gcgttcgtag cttctgttgc cgcattgact tgttggctgt ctgcgcctat
     9721 gtatacaagg gaacctaaaa tgatagatgc tgtacccatt gtaatctttt tcatagctga
     9781 tgaacgttgt tctgattgat acttactgtt taaataatgt tttgtcatgt tgttttcctc
     9841 ctaaggatac aaattatgtg ttgttatatg agatgattaa ccattgcaat tgataatggt
     9901 tatcaattat actagcaca
//
LOCUS       DAHHYJ010000098         9735 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000098 DAHHYJ010000000
VERSION     DAHHYJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.098"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(221..1102)
                     /locus_tag="NBK22_001877"
     CDS             complement(221..1102)
                     /locus_tag="NBK22_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD2415061.1"
                     /translation="MTNLLMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGVKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
     gene            complement(1080..1310)
                     /locus_tag="NBK22_001878"
     CDS             complement(1080..1310)
                     /locus_tag="NBK22_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCD2415062.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            complement(1303..2640)
                     /locus_tag="NBK22_001879"
     CDS             complement(1303..2640)
                     /locus_tag="NBK22_001879"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCD2415063.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            complement(2657..3046)
                     /gene="nusB"
                     /locus_tag="NBK22_001880"
     CDS             complement(2657..3046)
                     /gene="nusB"
                     /locus_tag="NBK22_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCD2415064.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            complement(3106..3468)
                     /locus_tag="NBK22_001881"
     CDS             complement(3106..3468)
                     /locus_tag="NBK22_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD2415065.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            complement(3483..4838)
                     /gene="accC"
                     /locus_tag="NBK22_001882"
     CDS             complement(3483..4838)
                     /gene="accC"
                     /locus_tag="NBK22_001882"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD2415066.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAKKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGDVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            complement(4838..5302)
                     /gene="accB"
                     /locus_tag="NBK22_001883"
     CDS             complement(4838..5302)
                     /gene="accB"
                     /locus_tag="NBK22_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCD2415067.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQVQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            complement(5776..6333)
                     /gene="efp"
                     /locus_tag="NBK22_001884"
     CDS             complement(5776..6333)
                     /gene="efp"
                     /locus_tag="NBK22_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCD2415068.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            complement(6359..7420)
                     /locus_tag="NBK22_001885"
     CDS             complement(6359..7420)
                     /locus_tag="NBK22_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD2415069.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQEKFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGQRGALPHGVASDKIIEKGNMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            7525..8106
                     /locus_tag="NBK22_001886"
     CDS             7525..8106
                     /locus_tag="NBK22_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415070.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISESKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            8120..8338
                     /locus_tag="NBK22_001887"
     CDS             8120..8338
                     /locus_tag="NBK22_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415071.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMHKYKRNQRRK"
     gene            complement(8402..9232)
                     /locus_tag="NBK22_001888"
     CDS             complement(8402..9232)
                     /locus_tag="NBK22_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD2415072.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPELHPNMP
                     STVTEAYRVISQGLLEGFKNLGFNTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDKHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            9390..>9735
                     /locus_tag="NBK22_001889"
     CDS             9390..>9735
                     /locus_tag="NBK22_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD2415073.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKG"
BASE COUNT         3024 a         1766 c         1346 g         3599 t
ORIGIN      
        1 ataagcgtca attgtttgaa catatttaat taaaatcaca ttgatatcac agatacgaat
       61 attgtcgtat agaaattgaa aattctattt tttaaatgaa agtcttcaac ataattttaa
      121 gtttcaacat gagaaaaatc gattaacaaa caacgtcagt tgaatatgcc ttttgagaca
      181 tttcaaactt tacaattgtt gctaatcgat atatttgctt ttagtgatcc ctgctataaa
      241 ataaatcaac gatttctaat aagtgttttg tattgaattg ttcatcaatt tgcgttagtt
      301 catccactgc tgcgtctcta tgataagtca atttatcttc tgcgccatct ttccctaata
      361 aactcacgta cgtactttta ttattttcaa gatcgctgcc cactttttta cctaactttg
      421 cttcatcacc atagcagtct aataaatcat ctttaatctg gaacatcata cctaaatgat
      481 aactataact ttctaaatgt tctttagttg catcatcgac attagcgata tctgctgcac
      541 tcataaccgc aaaagttaat aatgctcctg tttttgtttt gtgtatcatt tccaaagttt
      601 caagatcaat tggttggcct tcgctttgca tatctaacat ttgaccgccg accattccaa
      661 catgaccact tgctattgac agccgttgta gaacttttat ttttacttca tcagttaatc
      721 tatcatcact tgaaataagt tcaaatgctt tagttaataa agcatcacct gctaatatcg
      781 cagtccactc accatatact ttatgatttg ttaatttccc tcgtcgataa tcatcattat
      841 ccatcgctgg taggtcatca tgaataagtg aatatgtatg aatcatttct agtgcaattg
      901 cgctcttcac acctaactca tactcggtat ttagtgaatc taaagtgagt aataacagaa
      961 ctggtcggat gcgtttacct ccagcattta atgaatacaa catgctttct tctagctgag
     1021 tatccattac tgatttattt atcgcaaccg ataattcatt attgacttca tctattaatt
     1081 tattcatcag tagattcgtc attttttaca tcctcagctt cttcttttat taagtcattc
     1141 accttttttt cggcattttt taaagttgtg tcacaagctg ctgatagttt cataccacgt
     1201 tgatataaat ctaatgattc ctctaaagat actgtttcat tatctaattt ttgaacaatt
     1261 tgctctaatt cttgcatcat ttcttcaaaa ctttgcgttt ctttagtcat tattacacct
     1321 tactttcgta acttttgcat ctactaagcc atctttcatt gttaacgtca attgatcatt
     1381 ttctgttaaa tctttagtac tcgtaatgac ttcgtctttt ttattaacaa ttgcatatcc
     1441 acgcaacatt gtattagttg gacttaaatt gtttaagttt tctactttat ttttcaaatc
     1501 atttttataa cttaatatct tagaattcaa taatttaaca agttggtttg tcaattgaag
     1561 attattttgt tgttcttgat taacactact tagtaatgct tttaaattat aacgttgttg
     1621 caacagcatt aatcgatgtc tctgttgttc aaaagttgcc tgaatttgtt gtttcagtct
     1681 cttttctaaa tcatctcgac gttgtatctg ttgatcatac aataaagttg gttgtttaaa
     1741 cttgtaatac gatgacaaat gttcaacatg tttacgttgt tgttctaaat gtttcttgat
     1801 gaaacgagtc aatgtaaact gatattgctg tatttgttgc agcaattcat attggtctgg
     1861 tgttgcaata acagcagctt gagttggagt cgcagctctg atgtctgcag caaaatcact
     1921 taatgtaaag tctgtttcat gacctactgc tgatataatc ggtgtcttac aattatatat
     1981 tgcacggacg acagcttctt cgttaaaatt ccataagtct tctatggatc caccgcctcg
     2041 acctacaata atggtatcta cacctaaact atctgcatat tcaatttttt caataatgtc
     2101 gtcttttgct ttttcacctt gaaccaaagt actaatttgt atttgttcag ctaatggaaa
     2161 acgactattt atcgttgaat ggatatctcg aattgcggca cctgtactcg ctgttaaaac
     2221 tgcaattttt ttaggaaact taggtattga tttcttattc gctttatcaa aacaaccttc
     2281 ttcagttaat tttttcttta atgcttctaa tttttgatat aagttcccta taccatctaa
     2341 ttgcatttta tttacataaa tttgatagtt tccacgacgt tcaaaaacag aaacacgtgc
     2401 ttctaataag acttcatctc cttctttagg ttcgaagttt aatttagaag cactaccttt
     2461 gaacatcatg gcacttataa cgctttcttt atctttcaca ttaaaatata aatgaccact
     2521 tgaatgcttt ttgaaatttg aaagctcacc tttaatcaat acagattgga gatgtggatc
     2581 ttgatcaaat ttatatttaa tatatttcgt taaagctgaa acacttaaat aatctgacat
     2641 ataacatcac tcaattttat ttttttatat tactcaatac accatttata aatttataat
     2701 gatcatcatc actgaattgt tttgttaatt caactgcttc attcattacg actttagcag
     2761 gtgtatcact gtgtaatatt tcatatgttg ccattcttaa aataatacga tccgttttta
     2821 ataaacgtgc aatagtccaa tcttttaaat aaggactaat tgtctcgtct aatacaggtt
     2881 cgtgatcttt aacgccagaa actagccaat gaataaattc gaagtctaaa tctggattat
     2941 cgtctttaat aaagcttatc gcttcattta tcgttaaatc actgtccttc atttctaatt
     3001 gaaataaagt ttgaaaagct tgcactcggg attctttacg actcattttt aactccttca
     3061 aacgtttgta tttttcttta tttaattact gaattaggta tgacattact tttcaataac
     3121 gatttgtgta atgtgaatat taatttgctt aggttctatc gctgtcatat tagaaattga
     3181 attaaaaatt gacgtttgaa ttttgtttgc agtttttgaa atattaacac catgttttaa
     3241 tgcacaatat acatctatat atatgccatc ttctttactc tcgattttta aatcacggct
     3301 taaattttta cgactaactt tttctaaatt tgtttctttt aattcagcaa aatggccagt
     3361 gatgccttcg acttccgaag tagctatact tgcaataaca gatagcactt ctggcgctat
     3421 ttctacttta cctaattttg aatttgaata atcagttact ttgaccatgg attgacctcc
     3481 tattaacctt catcattcat aatgctattt tgctctaaaa agtttgtatt aaatttaccg
     3541 cttctaaata tatcgttatt caataattta atatggaatg gaatagttgt atcaatacca
     3601 agaaccacaa attcacttaa tgcacgaatg ccagccataa ttgcttcatc tcgtgtcggt
     3661 tcatgtatga ttaatttcgc taccatcgaa tcataatatg gcggtatcgt ataattagta
     3721 taacatgctg attctattcg aacaccatat ccacctggtg caagatattg ctcaatttta
     3781 cctggtgatg gcataaagtt cttgtaagga ttttcagcat taattctaaa ttcaattgcg
     3841 tgtcctgtta atttaatatc ttcttgttta tacggtaaca cgtcacccat agcaacttgt
     3901 aattgtaatt taactaaatc aattcctgtt accatttcag ttacaggatg ttctacttga
     3961 atacgtgtat tcatttccat aaaataaaat ttattatcat ttaaatcata tataaactca
     4021 attgttcccg cattttcata atttacagct ttcgctgcac gaactgcggc atttcccatt
     4081 tcacgacgtg tttcatcatc taaaattggg gaaggtgctt cttccactaa tttctgcata
     4141 cgtctttgaa ttgtacaatc acgttctcct aaatgaatta cattaccata gctgtcccca
     4201 acaatttgga tttcaatatg gcggaagttt tcgatgaatt tctccatata aagtccacca
     4261 ttaccaaatg cagtttgagc ttcttgttct gtcattcgga agccagtttc aagttctttt
     4321 tcatcacgag caacacggat accttttccg ccaccgccag cagtagcttt aatgatgacc
     4381 ggatagccaa tttttttggc gattttctta gcttctgaga cgtctttcat taaaccgtca
     4441 ctaccaggaa caactggaac attggcttta atcatttctg ccttagcaac atctttgata
     4501 cccatttttt ggatagattg ataacttggt ccaatgaact tcaattggca tgcttcgcat
     4561 aattctgcaa agtcagcatt ttcagctaaa aagccataac ccggatgaac gccatcacaa
     4621 cctgtagaag ttgcaataga taagatgttc ggaatattta aatatgaatc tttagacaaa
     4681 gtgggaccta cgcaatatgc ttcatcagca atttgagtat gtagcgcatc tttatcccct
     4741 tcagaataga ttgcaacagt ttggatgcct aaatcacgac aagcgcgaat aatcctaact
     4801 gcgatttcac cgcggtttgc aattaaaacc tttttcatta tttcacctta aataacggtt
     4861 ggccatactc taccatttgt ccgtcttcta ctaagatttc aacaatttca cctgaaattt
     4921 ctgcttgaat ttcattaaat agtttcattg cctctaaaat acacactgtt gtttcatttg
     4981 aaacagtgtc cccaacttgc acatatgctt cttcgtctgg agatggcgat ttgtaaaatg
     5041 tacctaccat aggtgcatta attgttttgt gattatctga agttggcttt ggagcttcag
     5101 ttttattgct atcagttgat tgtacttgag gcataggcat tgccgcagct tcaactggca
     5161 tttgtgagat ttgtggcgtg ataatctcag tttctttttc tttcttaagc gtcactttgc
     5221 ctttagtatc ttcaatattg atttccgtta aagttgattt atccagaatt tcaattaatt
     5281 ctttgatttc tttaaagttc attattactg actccttcag tttgttttca tctacccgtc
     5341 tattttactt gagacaactc ttcaattcaa gcatgttcat attgctggcg acattataag
     5401 tctatcccaa agttataata aaaccacatt ttaaattaaa aacacttgtg tatttattac
     5461 ttaacattga ctcatcttaa ctcttgatta aaaattttat gcattcagtt aaacatatca
     5521 atgttatttt tattagctat tatgttttat cctcaacaat atgatatgta gatatatacc
     5581 tttactcatg tctatgtttt ttcactacca attagacgat tcgttttata ttttaaaatt
     5641 taatttaaaa ataaaaagcc tcatatcagc atataaccta taatcatttt aggcaatagc
     5701 tttatgagac ttatttagaa tatcttacgt taaatcagta tagtgaatac aagctatttg
     5761 ttaacaaatc agagattatc ctcttgaaat gtagcttcca tcaccagtgt tgataattaa
     5821 aacgtcacct tcgtttacaa ataaaggtac atttaatgta taaccagttt caacagttgc
     5881 cgatttagta gcaccagttg cagtatcacc tttaatacca ggttctgttt cagttactgt
     5941 taattcaaca gttttaggta attcaacacc gatagtttca ccttcgtatg tttgaatttg
     6001 tacttccata ccttctttta agtaattcaa ttcttctttt aagtaatcac ttgaaagttc
     6061 tgtttgttca aagctttcat tatccataaa tacatgatta tctccgtcag catataaata
     6121 ttgcatgcga cgattttcaa tcattgctgg ttcaactttt tcaccagctc taaacgtttt
     6181 ctcttgaatt gcaccagttc ttaaattacg taattttgaa cgaacgaatg ctgaaccttt
     6241 accaggcttt acatgttgga agtctataac tttccaaata gcgttatcaa cagaaattgt
     6301 taaacctgtt ttaaaatcat taaccgaaat cattcagttt cctcctcatt ttacacgctt
     6361 atgttaaaac tataaggtct tttgtgcatt tagtaaagac ttgacaacca ttttctgtaa
     6421 ttaaaatatc atcttctatt cttataccgc ccaaaccttc tatataaaca ccaggttcta
     6481 ctgtaacaca gttgttaact tgaagtttat cttgtatcgt acgagccagc attggccctt
     6541 catggatttc taaaccaata ccatgtccta gtgaatgtcc aaattctttt ccataccctt
     6601 ttgactctaa atagtttctt gaaatggcat cagcttctgc accagtcatg ccaggtctaa
     6661 tctcattaat tgctttcatt tgagattcaa gtactatttg atatatttct ttcagtttag
     6721 gatctggttc tccaatagca aatgttctag taatatctga acaatagccg ttataatatg
     6781 cgccaaaatc taatgtaatc atgttgcctt tttcaataat tttgtcactt gcaacaccat
     6841 gtggtaatgc acctctttga ccagatgcta caatcgtatc gaatgatggt ccatctgctc
     6901 ctaattctag cattttactt tctaatattg cctttaattc tttttcagtc atacctgctt
     6961 ttacaacagt taaaatatat tcatatgttt catcaacaat attagctgct ttttgaatta
     7021 aagcaatttc gtcagcatct ttgacgtctc taattttatc tacagtatta gaaatgctta
     7081 ttaatgatat acggctttta tttaattcaa ggtatgtatc ataacttaca tgatgcccct
     7141 caaaacctac attttcaaat ttttcttggt gtagcaattc tttaatctca ccaataatag
     7201 tagatttacg attaataatt tcataatttg gcgcctgctt agttgcttga tcaatatatc
     7261 taaagtctgt tatcaaatat tgtttatctt tagatatgat aagtgctcca ctggtaccag
     7321 taaaacctga taaatatctt ctattgtaat ccgaaagaat gataatcgca tctaaatgtt
     7381 tttgttctaa aatacgatgc acttgcgtta ttctgctcat cttattacct cctagtgaaa
     7441 cgtttgtgca tttcaacttt atacattaaa ataatatcat aataaggata aaaaataata
     7501 gatattgatt ttagggagat agtaatgaaa aaattggttt caattgttgg cgcaacatta
     7561 ttgttagctg gatgtggatc acaaaattta gcaccattag aagaaaaaac aacagattta
     7621 agagaagata atcatcaact caaactagat attcaagaac ttaatcaaca aattagtgag
     7681 tctaaatcta aaattaaagg gcttgaaaag gataaagaaa acagtaaaaa aactgcatct
     7741 aataatacga aaattaaatt gatgaatgtt acatcaacat actacgacaa agttgctaaa
     7801 gctttgaaat cctataacga tattgagaaa gatgtaagta aaaacaaagg cgataagaat
     7861 gttcaatcga aattaaatca aatttctaat gatattcaaa gtgctcacac ttcttacaaa
     7921 gatgctatcg atggtttatc acttagtgat gatgataaaa aaacgtctaa aaatatcgat
     7981 aaattaaact ctgatttgaa tcatgcattt gatgatatta aaaatggcta tcaaaataaa
     8041 gataaaaaac aacttacaaa aggacaacaa gcgttgtcaa aattaaactt aaatgcaaaa
     8101 tcatgatagg agtcttttaa tgcgtaatat aatattttat cttgtactta ttattgctgc
     8161 gattggatta gtaatgaatc tagatgcctt tattttttca atcgtcagaa tgttagtcag
     8221 ctttgctgta atagctggta ttatttatct gatttattat ttcttcatct taactgaaga
     8281 ccaacgcaaa tatcgcaaag caatgcataa gtataaaaga aatcaaagaa gaaaatagat
     8341 aaaaaaacgg aagcacttgt aggtaaaata gtctacgtgc ttccattttt tattctaaaa
     8401 actactttct aaacatccat tcatctgaac gatatttttc agttaattct tccacttctg
     8461 ccaattgagc ttctgttaat tcaagtggct ttaattctat atttaaacct ttcttaaaac
     8521 ctttctcgaa agcttcttcc atttgactaa tagtaatgtg tttatctgaa atatcattga
     8581 tggcaactgc tttttcaacg aatgcctctt tcatttttaa ttttaatctt tcatttttat
     8641 aaataaacat atcaaacagt tcatcaatat caatatcttg taaaatcgaa ccgtgttgga
     8701 ggattacgcc cttttgtctc gtttgagcac tcccagcaat cttacggcct tcaacaacta
     8761 gctcatacca acttggtgca tcaaaacaca ctgaacttcg aggttgtttt aatttttgac
     8821 gctcttcagg cgttttaggt accgcaaaat aagtattaaa tcctaagttt ttaaatcctt
     8881 ctaataatcc ttgtgaaatc actctgtacg cttctgtaac tgtagaaggc atattcggat
     8941 gcaattcagg cacaatcaca ctgtaagtta actctttatc atgtagcacc ccacggccac
     9001 cagtttgacg ccttacgaga ccaaaacctt tctctttaac cttatcaata tcaatttctt
     9061 tttgtagcct ttggaaatac cctattgata atgttgcagg attccatgtg taaaaacgta
     9121 taactggatc aatttcacct ctagagacaa aatttaataa cgcttcatcc attgccatat
     9181 tataatatgg gtctttactt cctgtattaa taaaattcca agtttcagtc atattcaaga
     9241 actcctatca gcaaaatgta taatatttga ttcgctaatt aatcaattta actaaatgaa
     9301 taataattgc aattctttag tgaaatattt tgataatttg acctaacagt cttataatta
     9361 tattatcgtt taattaggga ggatgcaaga tgagtgctag tttgtacatc gcaataattt
     9421 tagttatagc aattattgct tatatgattg ttcaacaaat tcttaacaag cgagctgtta
     9481 aagaattaga tcaaaatgaa ttccataatg ggattagaaa agctcaagtc atcgatgtta
     9541 gagagaaagt tgactatgac tacggtcaca ttaatgggtc tcgcaatatt cctatgacaa
     9601 tgttcaggca acgattccaa ggattaagaa aagaccaacc ggtatactta tgtgatgcca
     9661 atgggattgc tagctataga gctgctcgta ttttgaaaaa gaatggatat acagatatct
     9721 atatgttaaa aggcg
//
LOCUS       DAHHYJ010000099         9610 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000099 DAHHYJ010000000
VERSION     DAHHYJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9610
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.099"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            90..725
                     /locus_tag="NBK22_001890"
     CDS             90..725
                     /locus_tag="NBK22_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCD2415074.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            1029..2108
                     /locus_tag="NBK22_001891"
     CDS             1029..2108
                     /locus_tag="NBK22_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCD2415075.1"
                     /translation="MWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYAL
                     LIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRAG
                     EGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVILT
                     IITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIVIGLIALLAWPM
                     SASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKWR
                     LPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTMS
                     HFIFVRPMKKVQQQSAKVKQQTQIV"
     gene            2168..2392
                     /locus_tag="NBK22_001892"
     CDS             2168..2392
                     /locus_tag="NBK22_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCD2415076.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            2600..3850
                     /locus_tag="NBK22_001893"
     CDS             2600..3850
                     /locus_tag="NBK22_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCD2415077.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGNGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDNIAMLAFTNTIISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIVLVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            4033..4983
                     /locus_tag="NBK22_001894"
     CDS             4033..4983
                     /locus_tag="NBK22_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2415078.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTKIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQVEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKDVMKPHHIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            5132..6616
                     /locus_tag="NBK22_001895"
     CDS             5132..6616
                     /locus_tag="NBK22_001895"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCD2415079.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEYNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYSEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKITLDRSDSGLLPTNVEGTSRSTILDTPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            6613..7572
                     /locus_tag="NBK22_001896"
     CDS             6613..7572
                     /locus_tag="NBK22_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCD2415080.1"
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLVDSPMRDAHYQLITKTLNANGT
                     VVFDPNIRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENEAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(7760..7869)
                     /locus_tag="NBK22_001897"
                     /pseudo
     CDS             complement(7760..7869)
                     /locus_tag="NBK22_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7941..8657)
                     /locus_tag="NBK22_001898"
     CDS             complement(7941..8657)
                     /locus_tag="NBK22_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2415081.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSRDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(8676..>9610)
                     /locus_tag="NBK22_001899"
     CDS             complement(8676..>9610)
                     /locus_tag="NBK22_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD2415082.1"
                     /translation="YVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSL
                     FFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYE
                     YTLKIEAINNEMRKFRHDYVNILTTLSEFIREDDMPGLRDYFNKNIVPMKDNLQMNAI
                     KLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILD
                     NAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLG
                     LSTLKEIADNADNVLLDTIIENGFFIQKVEIINN"
BASE COUNT         3338 a         1313 c         1782 g         3177 t
ORIGIN      
        1 tatttgtttt ttaataatct aacaattacg ctctaactcg tttgaataca tagatataat
       61 tcattcaaac acgaaggagg aaatagcgaa tgagtgacca agttaaaatt cctcgagcaa
      121 ctttaaaacg tttgccgtta tattatagat ttgtcagttc attaaaatct aaaggtatag
      181 atcgtgtaaa ttcaaaagcg attagcgatg cgttacaaat tgactcggca acaattcgtc
      241 gtgacttttc atattttggc gaattaggta aaaaagggta cggatataat atagatagtt
      301 tattggattt ctttaaatct gaactaagcg agagtgacat gatcaaaatc gcaattgtcg
      361 gagttgggaa cctagggaaa gctttgctca catataactt ttcaatacat gacgatatga
      421 cgattacaga agcgtttgac gtaaaagaag atgttattgg ccagaaaata gggaacgtta
      481 ttgtaaaaga taacgatgaa ttaataacaa cattgaagaa ggaagaaata gatgttgtga
      541 ttctaactac accagaaaga gttgcacaga aagttgcaga tgaactcgtc caagctggtg
      601 tgaaaggtat tttaaacttc actcctggta gaattaatac gccttcagat gtgcaagtac
      661 atcaaattga cttaggtata gaattacagt cattattatt ctttatgaaa aattacagtg
      721 aataaatgtg atgttagctt tgaatgatta accttgttat atctttaatt aattatagaa
      781 cagtctggga ctaagtatgt ctcaggcttt ttctgttatg tttgaaaatg aaaggaaaaa
      841 ctattgtgct gataaatata ttataggtta ataaattaac acgtagagat ataatgatga
      901 atcgtaatta atcgccttcc ttatatgaat ataatataaa tatcaaaaat attgtgtata
      961 aggttgaaaa caagaaaatc atcacctata atacatacta aacttatcgg aaatgagggg
     1021 ttaggattat gtggatgatt tttagtggct taattgtcgg gggactctta ggttttgtca
     1081 tgcaaagaac aagattttgt ttaacaggtg gctttcgaga tatgtatgtg caaaagaata
     1141 ataagatgtt ctatgcatta ttaatcgcta ttactattca aagtatagga ttattgattt
     1201 tgacggcaac agatatttta caaattcctg cacatagttt tccaatattg ggaacaatta
     1261 taggttcttt tatatttgga attggaatag tattggctgg agggtgtgca acaggtactt
     1321 ggtatcgcgc tggtgaaggg ctaattggta gttggattgc attggtatta tatgctgtta
     1381 ctgcagcaat cactaaaaca gggattttaa agccagtaat ggataaaatt aatcaaccaa
     1441 cgaatgtaaa tagtgatatg tctcaaacaa ctggcattcc gttttgggga ttagtcgtta
     1501 tattaactat aatcaccatt tttctagttg taagaacact caataataag aaagttcgag
     1561 ttgctgttcc aaaactaaag caacgttata caggtattag atattacctt ttcgaaaaac
     1621 gataccaccc atttattgca gcaattgtaa ttggacttat cgcactctta gcttggccaa
     1681 tgagtgcatc aactggaaga aatgacggtt taggtataac aacgccatca gcaaatttag
     1741 tacacttttt gattacaggt gaaactaaat ttattgattg gggtgtcttt ttagttctag
     1801 gaattttcat tggttcatat attgcagcta gaggatcaag agaatttaaa tggcgattgc
     1861 cagacaagat tacaatacga aacagtgcta ttggtggcat atgtatggga tttggtgcgt
     1921 cagttgctgg tggttgttct atcggtaacg gtttggttga aacagcaacg atgacttggc
     1981 aaggatggat tgcgctagca tcgatgatag ttggtgtatg gacaatgagt cattttatct
     2041 ttgttcgtcc aatgaaaaaa gtacaacaac aatcagcaaa ggttaaacag caaacgcaaa
     2101 tagtatagaa gattattatg caaatgatgt tgatcaaata aaagtgattg gaaaaggaga
     2161 aataattatg atacacgaat taggtacagt aggaatggta tgtccatttc cgttaattga
     2221 agcgcaaaag aaaatggcaa cattgcaatc tggagatgaa ttaaaaattg attttgattg
     2281 cacgcaagcg acggaagcta ttccaaattg ggcagcagaa aatggatatc ctgtaacaaa
     2341 ctatgaacaa attgataatg cttcatggac aattacaatt caaaaagttt aacgttatca
     2401 ttttaacaat aaaatagata ttagattcta tggctacttc cgctaattta aaagtgagta
     2461 agtagtcttt tttgttttag ttcatgaaat catttttata tagtgtggca cattttattc
     2521 caaaagatgt aataaaactt aacgcatttt tgctttttat aaattgtcag attattatga
     2581 aaacaaggga gtggtaagta tgaatcttaa cgatacgata tttatgtttt tgtgtacatt
     2641 attagtttgg ttaatgacac caggattaag tttattttat ggtgggttag ttcaatctaa
     2701 aaatgcgctt aatactgtca tgcaaagtat ggcagcaatt gtgcttgtta catttgtatg
     2761 gataacagtt ggttttacaa ttagttttgg aaatgggaat ttatggttcg gaaattggga
     2821 atatactttt cttaatcatg taggttttgc gactcaagaa gatattagcc cacatattcc
     2881 tttcgctttg tttatgttat ttcaaatgat gttttgtacg attgcaattt ctattttatc
     2941 tggttcaatc gctgagaaaa tgaagtttat tccttattta ttattcgtag taatatggac
     3001 tgctcttgta tacagtccag tagcacattg ggtttggggc ggcggttgga ttaacaaact
     3061 cggtgtatta gatttcgctg gaggtacggt tgttcatatt acatcaggtg tttctggttt
     3121 agtattagct attatgattg gaaaaggaaa caaacattct gaatcaacac cacataatct
     3181 tatcattacg ttgattggcg gtatattcgt gtggattggt tggtatggat ttaatgtagg
     3241 tagtgctttt acatttgata atattgcgat gcttgcattt acaaatacta tcatttcagc
     3301 cagtgcaggt gctataggtt ggttaatttt agaatatatt tttaaaaaga cgacaagttt
     3361 acttggactt ttactcggtg cattagctgg tttagttgtc attactcctg cagcaggata
     3421 tgtaacatat cttagtgcaa caataatggc tttaatagga ggtatctgtt gttatattgt
     3481 cattaattac atcaaggtaa aactaaaata tcatgatgca ttagatgcat ttggtattca
     3541 tggtgttggt ggtattattg gtgctgtttt aacagcagtt ttccaaagta aaaaggcaaa
     3601 tcctgacatt gagaatggct ttatttatac tggtgacata catattgtac ttgtacaaat
     3661 attatgtgta acagcagttg taatttttag tatcgtcatg acgtttatta ttgcgaaagt
     3721 aattaaatta attacaccat tatctgttac ggaacaagaa acgaatatag gattagacaa
     3781 gattgttcac ggtgaacatg cttactttga aggtgagcta aatagattca ataaacatat
     3841 tcgatattag aatatattta catagaatat tcattgtcct gacatttaac taaaggttga
     3901 tgttgggaca ttttgttata caaaagtttt attttgaaat ctttttatga aagaagcaga
     3961 aatattattt aaagcggtta cacatatgct aaaataaggc taagtgtcac aaataatgat
     4021 aggtgaataa gtatgaaaaa tatatctgat attgccaaat tggcaggcgt ttcaaaaagt
     4081 acagtatcta gatttttaaa taatggatct gtcagtaaaa aaacaagtga aaaattaaca
     4141 aaaattatag cagaacatga ctatcaaccg aatcaatttg ctcaaagttt aagagcgaga
     4201 caaacacacc ttattggtgc cattatacct agaatgaatt catatgcagt agacgaaaca
     4261 atcaaaggat tggcaaaaca atgccaaaaa tatgaatcac aattaatttt aaattataca
     4321 ggtttaaata tcgaagcaga aatacaagcg cttgaaacat tagcacgcag taaagtagat
     4381 ggtattgttt taatggctac agatataaca gagagacata ttgaagtcat taataaaatg
     4441 aatgtaccaa tcgttattgt tggtcaacaa catgaacaac ttcatagtat tgtgcatgat
     4501 gattataaag caggtcaaat tataggcgaa tggattggtc aacagggata tcaacaagtt
     4561 gaagtgttta gcgtaagtga aaaagatatt gcagttggta tacatagaaa acgtggttta
     4621 cttgaccagt tagctaaata ccaaattaaa cctaatatac atgaaacaaa ttttacttat
     4681 gtggaagcac aaaaagatgt tgcaaatgtt ttggaaaatg tggagcaagt agatgcggtt
     4741 gttggagcaa ctgatacgat tgcattagct gcctataaat attattctga taaaaaagat
     4801 gttatgaaac cacatcatat atatggtttt ggtggtgacc caatgacaca attagtgtct
     4861 ccatcgataa aaacaattca ttataattat tttgaagctg gccaatgcgc gatggaagag
     4921 atacaacaga tgcttaaaaa gcaagatatg ccatatagcg tcacagtaga tgttaatatt
     4981 tagacgctgt attttttaaa ataaatgtgg aaccgatacc atataactat aaatggatag
     5041 gttaaaagtt aaagaacgta ggtaaaattt gctataatag aatataaatt gttaacagca
     5101 taaattataa aaggaggact gggtaaatat tatgaccgaa tggactagag aagaacgtta
     5161 tcaacgaatc gaggacgttg atactgagta ttttaaaaca ttaaaacaac aagttgatca
     5221 atcaaaattt cgtcaacaat ttcatataca accagaaaca ggcttattaa atgaccccaa
     5281 cggacttatt ttttataaag ggaagtatta tgtttcacat caatggttcc cattaggcgc
     5341 agtacatggc ttaaagtatt ggtataacta cacgagtgat gacttaataa actttaaagc
     5401 tgaagggcca attttaaatc cagatactaa atatgacagc catggtgtat atagcggtag
     5461 cgcttttgaa tataacgggc atttatatta tatgtacaca ggaaatcatc gagataatca
     5521 ttggcaacga catgcgagtc agatgattgc acgattgaaa gaagacggtt cagtagaaaa
     5581 gtttccaaag ccagtaatta gccaacaacc agaagggtat acgagtcatt ttagagatcc
     5641 taaagtattt aaatatagtg agaaatatta cgcgatcatt ggcgcacaaa ataatgatca
     5701 gcaaggtcga ttattacttt ataatactga agatataatt aattggcatt atttaggtga
     5761 aataaataca gagttggatg attttggata tatgtgggaa tgcccagatt actttaatgt
     5821 agataatcaa gatgtcatac ttatttgtcc acaaggtatt gaaccaaaag gcgatcagtt
     5881 caaaaatatt tatcaaagtg gttatatact tggaaagttt gatattgaaa agttaacata
     5941 tgaacatgaa aattttgtcg agcttgataa tggctttgat ttctatgcac ctcaaacatt
     6001 tttagatgaa aaaggccgac gagtactaat tggatggatg gggttaccgg aaatcgaata
     6061 tcctactgat aatgaaggat gggcccattg cttaacaatt cctcgtgtat taaatgtaga
     6121 aaatggtcaa cttaagcaac gtccatatcc agcgttggaa aaattacgtc acaataaaga
     6181 gacagcatta ggctatgcaa ataaatttac tcgaaaatta catccgtatg aaggtaaaca
     6241 gtatgaatta atcatagata ttttggataa tgatgctacc gaagtgtact ttgaattacg
     6301 tacatctaag acttcttcaa cattaattgc ttataacaag cgtgaaaata aaataacatt
     6361 agatcgcagc gacagtggtt tattgccgac aaatgttgaa ggtacgtcgc gtagtacgat
     6421 attagacacg ccattaaagc aattacaaat ttttgttgat acatcaagta tagaaatttt
     6481 ctgtaatgat ggggaacgtg ttttgacatc tcgaattttc ccgacagaag acgcactagg
     6541 tattaaaaca tcaacagaat cagggcaagt atatttacaa tttactaaat atgatttaaa
     6601 ggatgaacat aaatgagacg tctattttca ataggagaag cgttaattga ttttattcca
     6661 aatattacaa atgcgaattt aaaagatgtt caaacattta cgaagcaaat tggtggcgcg
     6721 ccatgcaatg tagcttgtac agttcaaaag ttaggacaac aagcgtatat gattacacaa
     6781 ttaggtaatg atgcatttgg agatagcatt attgaaacga tatcaagtat tggtgtagat
     6841 gtatcaaaag tgtatcgcac gaatgaagca aacactgcgt tagcttttgt gagtttgact
     6901 gaagcgggcg agcgagattt ttctttttat cgtaagccct ctgctgatat gttatttgaa
     6961 ccaagttttg tgaatgatat tgatgtgaat gaaaatgatg tagtacattt ttgttctgtt
     7021 gatttggtcg atagtccgat gagagatgca cattatcaat tgattaccaa aacattaaat
     7081 gcaaatggta ctgtcgtatt tgatcctaat ataagattgc cattatggga taacgctgaa
     7141 gatttaagac aaactattca tacctttttg ccattggcgc atattgtaaa agtttcagat
     7201 gaagaattag aatttattac aggtattcat gatgaaaatg aagcgattca atcattattt
     7261 acaggtaatg ttactgtagt catttatacg aagggagcag atggtgctgc cgtatatttg
     7321 aaaaatggta ttaatcatta tcatagtggc tataaggtaa aacctgttga tacaactggg
     7381 gcaggggatg cttttattgg tgcagttatt agcaggattt tagcaaccga tgtgttaaat
     7441 ttaactcagt tatttgaaaa tgaaggtgag gaaattttag cgtttagcaa tcgcgttgct
     7501 gcaatagtga caacgaagta tggtgctatt aatagtttgc caacattaca agaggttgaa
     7561 caagcatttt aaataattca tttataaaac agtgtaggaa acaagttgaa tcttgtgcta
     7621 cactgttttt taaaacaaaa taagattcat ggggtaggaa attgtagtat tttatttaag
     7681 gaaactgtcg atttcaaatt tagtatattt tgttaaaaat tgcgccatag gattgtagag
     7741 tgaaatagaa attgataaat tatgtagtaa taggaatagg tatacaaaga aacgttacat
     7801 cattttttaa ttcacgcgtc atatttaatt ttgttattaa acaattaaaa agccagctat
     7861 acagtgcatt tgctagttat cttgttaaaa tccaacaaga tttacaattg aatacgccgt
     7921 taactgactt tattatctta ttatattttt ttaacgtttc tcaccgatgc atagcagtgt
     7981 tctttatttt taaaatagac aattcgctct ttcgaatcta tagattcaat attatggcga
     8041 ttgacgacaa agctattatg acatctaaag aaacgatcat ctaattgact cagttctttt
     8101 aaattaccat aaaattcaat ttgacggtta tctaaatggg caatgagtct gtgcgatttt
     8161 gttgatgatt caaaaaacat aatatcatca tattgaacat acactgaatt actgccacgt
     8221 tttaattcaa tcgtttcaac gctattatct cttgataata attgtaagcg tgtatgtgca
     8281 gtttctaaac aatctataat tcgagttctt aattctgctg gatcatcttt aaaaataaaa
     8341 tccatcgctg caactttgta gacaaatgtt aaataggtaa gttcactgtg actcgtaacg
     8401 aaaataatgt taccaactgg gtcatgctta cgaatttcac tgcctaattt gataccatta
     8461 atatcagttg aaagttgaat atctaaaaag taacagccta tgtcattcat atttttagct
     8521 tgctcaagca cctcataagg attatcagtt gcgagggcaa tttccatagg cttttcttct
     8581 atcattatat aatttttaat aatggtaacc atgttttctc tttgttttgg atcgtcttcg
     8641 caaatgaaaa ttttcataca ttcacatcct tatggctagt tgttaataat ttcaactttt
     8701 tgaataaaga aaccattttc gataattgta tctaataaga cattgtctgc attatcagca
     8761 atttctttta aagttgatag acctaaacca cgaccttcac ctttagtaga aaaactttct
     8821 tggaacaatt catgaatgcg tggtatatca tcagcgcatt tattcataac aataaacgtt
     8881 actgaatttt cactttcaat aaatgcaacg cgaatgatag ggtcatcaat ttcagttgat
     8941 gcctcaattg cattatcaag aataatacca atactgcgac ttaaatcgat catattcaag
     9001 ttaatgctac ttacttcatc gggtatttcg atactaatcg gaatattcat ttcttgtgca
     9061 cgtaaaattt tcgcagtaat taagccttta atttcacgta ctttaagatt ctcgatacca
     9121 tttaatttta tagcattcat ttgtaaattg tctttcatag gtacaatatt tttattgaaa
     9181 taatcacgta ggccaggcat gtcatcttct cgaatgaatt ctgaaagcgt tgttaagata
     9241 ttgacataat cgtgacggaa cttgcgcatt tcgttgttga tagcttcaat cttcaatgta
     9301 tattcataat aggtttcaat ttcttcttga ttacgtttat atttcatctc tttaaggaga
     9361 aattgagaaa taacaaatgt taatatactt aaaaatatag tgataccaat aaaaataaaa
     9421 gaatactgcc ttattacttt agcttcatcc gagtttattt gtgaataaaa gaaaaataat
     9481 gaaaaagtaa gcagtaagat agtcgaaata actattaaaa atcctttgtt tagtattaga
     9541 tatggtgtgc taattttttt gagaactcta tttattatat atgagaatag tatactaata
     9601 gtcacataaa
//
LOCUS       DAHHYJ010000100         9515 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000100 DAHHYJ010000000
VERSION     DAHHYJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.100"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            50..559
                     /locus_tag="NBK22_001900"
     CDS             50..559
                     /locus_tag="NBK22_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2415083.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENDKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            571..1641
                     /locus_tag="NBK22_001901"
     CDS             571..1641
                     /locus_tag="NBK22_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD2415084.1"
                     /translation="MTKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEDLTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFANKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            1658..2272
                     /locus_tag="NBK22_001902"
     CDS             1658..2272
                     /locus_tag="NBK22_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD2415085.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFVYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPALK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLNRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGLFCIILSTLLLRNKLIN"
     gene            2389..2589
                     /locus_tag="NBK22_001903"
     CDS             2389..2589
                     /locus_tag="NBK22_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415086.1"
                     /translation="MTNPTRTKRCGGDVLCGIDNHFQLLLYISENHCQLETKTFFEYF
                     LRIVNKTHDYAILENKKICISH"
     gene            2666..2797
                     /locus_tag="NBK22_001904"
     CDS             2666..2797
                     /locus_tag="NBK22_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415087.1"
                     /translation="MLLPSSKSHIEMMCEMFKLRSFCENKDNNYSFYNSEKSVYDNN"
     gene            3059..3718
                     /gene="arlR"
                     /locus_tag="NBK22_001905"
     CDS             3059..3718
                     /gene="arlR"
                     /locus_tag="NBK22_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCD2415088.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            3715..5070
                     /gene="arlS"
                     /locus_tag="NBK22_001906"
     CDS             3715..5070
                     /gene="arlS"
                     /locus_tag="NBK22_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCD2415089.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKPVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINNEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            5354..8152
                     /locus_tag="NBK22_001907"
     CDS             5354..8152
                     /locus_tag="NBK22_001907"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCD2415090.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            8166..9434
                     /gene="sucB"
                     /locus_tag="NBK22_001908"
     CDS             8166..9434
                     /gene="sucB"
                     /locus_tag="NBK22_001908"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCD2415091.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNAFKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         3576 a         1341 c         1733 g         2865 t
ORIGIN      
        1 acatgtactg aaaaacatgt ttaacataat gatataagga gtgacgtaca tgatccgtct
       61 aggtaaaatg tcagatttag atcaaatctt aaatctagta gaagaagcaa aagaattaat
      121 gaaagaacac gacaacgagc aatgggacga tcagtaccca cttttagaac attttgaaga
      181 agatattgct aaagattatt tgtacgtatt agaggaaaat gacaaaattt atggctttat
      241 tgttgtcgac caagaccaag cagaatggta tgatgacatt gactggccag taaatagaga
      301 aggcgccttt gttattcatc gattaactgg ttcgaaagaa tataaaggag ctgctacaga
      361 attattcaat tatgttattg atgtagttaa agcacgtggt gcagaagtta ttttaacgga
      421 cacctttgcg ttaaacaaac ctgcacaagg tttatttgcc aaatttggat ttcataaggt
      481 cggtgaacaa ttaatggaat atccgccata tgataaaggt gaaccatttt atgcatatta
      541 taaaaattta aaagaataga ggtaatatta atgacgaaaa tcgcatttac cggaggggga
      601 acagttggac acgtatcagt aaatttaagt ttaattccaa ctgcattatc acaaggttat
      661 gaagcgcttt atattggttc taaaaatggt attgaaagag aaatgattga atcacaacta
      721 ccagaaatta agtattatcc tatttcgagt ggtaaattaa gaagatatat ttctttagaa
      781 aatgccaaag acgtatttaa agtattgaaa ggtattcttg atgctcgtaa agttttgaaa
      841 aaagaaaaac ctgatctatt attttcaaaa ggtggatttg tatctgtgcc tgttgttatt
      901 gcagccaaat cattaaatat accaactatt atccatgaat ctgacttaac accaggatta
      961 gcgaataaga tagcacttaa atttgccaag aaaatatata caacatttga agaaacgcta
     1021 aactacctac ctaaagagaa agctgatttt attggagcaa caattcgaga agatttaaaa
     1081 aatggtaatg cacataatgg ttatcaatta acaggcttta atgaaaataa aaaagtttta
     1141 ctcgttatgg gtggaagctt aggaagtaaa aaattaaata gcattattcg cgaaaactta
     1201 gatgcattat tacaacaata tcaagtgata catttaactg gtaaaggatt aaaagatgct
     1261 caagttaaaa aatcaggata tatacaatat gaatttgtta aagaggattt aacagattta
     1321 ttagcaatta cggatacagt aataagtaga gctggatcaa atgcgattta tgagttctta
     1381 acattacgta taccaatgtt attagtacca ttaggtttag atcaatcccg aggcgaccaa
     1441 attgacaatg caaatcattt tgctaataaa ggatatgcta aagcgattga tgaagaacaa
     1501 ttaacagcac aaattttatt acaagaacta aatgaaatgg aacaggaaag aactcgaatt
     1561 atcaataata tgaaatcgta tgaacaaagt tatacgaaag aagctttatt tgataagatg
     1621 attaaagacg cattgaatta atggggggta atgctttatg agtcaatgga aacgtatctc
     1681 tttgctcatc gtttttacat tggtttttgg aattatcgcg tttttccacg aatcaagact
     1741 tgggaaatgg attgataatg aagtttatga gtttgtatat tcatcagaga gctttattac
     1801 gacatctatc atgcttgggg ctactaaagt aggtgaagtc tgggcaatgt tatgtatttc
     1861 attacttctt gtggcatatc tcatgttaaa gcgccacaaa attgaagcat tattttttgc
     1921 attaacaatg gcattatctg gaattttgaa tccagcatta aaaaatatat tcgatagaga
     1981 aagacctaca ttgctgcgtt taattgatat aacaggattt agttttccta gcggtcatgc
     2041 tatgggatca actgcatatt ttggaagtgg tatctatcta ttaaatcgat taaatcaagg
     2101 taattcaaaa ggtattctta tagggttatg tgcagctatg attttattga tttccatatc
     2161 acgtgtatat ctaggtgtac attatccaac agatattatt gccggcatta ttggtggatt
     2221 attttgcatt attttatcaa cgttattact tagaaataaa ttaataaatt aaatagtaaa
     2281 aaaacaaaag cagtaaacct aaagtgtcgt aagggtttac tgcttttata aaacgttgtt
     2341 ataacgtata ttgtctttta cgggcatata aaaggggaat atttgaaaat gaccaatcca
     2401 acaagaacga aacgttgtgg gggggatgtt ctatgtggta ttgataatca ttttcaacta
     2461 ctattataca ttagtgagaa tcattgtcaa ttagaaacta aaactttttt tgaatatttt
     2521 ttaagaatag taaataaaac gcatgattac gctattttag aaaataaaaa aatttgtatt
     2581 tctcattaga attagaatat ttaaaagtga tgaggtttaa acattatatt gtttacatac
     2641 tccttttgaa ttcatacatt atgaaatgtt acttccaagt tcaaaatcgc acattgaaat
     2701 gatgtgtgaa atgtttaaac tacggtcatt ttgtgaaaat aaagataata actattcatt
     2761 ttacaatagt gaaaagtcag tatatgacaa caattaatat tgcggtaagg ccttgtgtta
     2821 cagtattcta tatttaagta ctgcaatcag aattaacaga atgccattaa ctgattatta
     2881 aatatttgag ttaataaata attaatgatt gtagcttgaa aaatttaaaa catggttatt
     2941 gatttgtgat aaaatttaaa cgtaaacaaa ctaatttaaa aagcaactat tgtatagaaa
     3001 aatacaaaat ttaaaatata ttaccttatt agaaaaagtc gtaatatgag gtgtacaaat
     3061 gacgcaaatt ttaatagtag aagatgaaca aaacttagca agatttcttg aattggaact
     3121 cacacatgaa aattacaatg tggacacaga gtatgatgga caagacggtt tagataaagc
     3181 gcttagccat tactatgatt taatcatatt agatttaatg ttgccgtcaa ttaatggctt
     3241 agaaatttgt cgcaaaatta gacaacaaca atctacacct atcattataa ttacagcgaa
     3301 aagtgatacg tatgacaaag ttgctgggct tgattacggt gcagacgatt atatagttaa
     3361 gccgtttgat attgaagaac ttttagcaag aattcgtgca attttacgtc gtcagccaca
     3421 aaaggatatt atcgatgtca acggtattac aattgataag aacgctttta aagtgacggt
     3481 aaatggcgca gaaattgaat taacaaaaac agagtatgat ttactatatc ttctagctga
     3541 aaataaaaac catgttatgc aacgggaaca aattttaaat catgtatggg gttataatag
     3601 tgaagtagaa acaaatgtcg tagatgttta tataagatat ttacgaaaca agttaaaacc
     3661 atacgatcgt gacaaaatga ttgaaacagt tcgtggcgtt gggtatgtga tacgatgaca
     3721 aaacgtaaat tgcgcaataa ctggattatt gttaccacga tgattacgtt tgtcacgata
     3781 tttttgtttt gtttaattat tatttttttc ttgaaagata cactgcataa tagtgagctt
     3841 gatgatgcag aacgaagctc aagcgatatt aataatttat ttcattctaa gcctgttaaa
     3901 gatatatctg cattagactt gaatgcatct ttaggtaatt ttcaagagat aattatttat
     3961 gatgagcata ataataaatt atttgagaca tcgaatgata acacagtgag agttgaacca
     4021 ggttatgaac accgttattt tgaccgcgta ataaaaaaac gctataaagg cattgaatat
     4081 ttaattatta aagaaccaat tacaacgcaa gatttcaaag ggtatagctt gttaattcat
     4141 tcactagaaa attatgataa catcgtaaaa tcattgtata tcattgcgct ggcatttgga
     4201 gtgattgcaa caattataac tgccacaatc agttatgtat tttcaacaca aattactaaa
     4261 ccgcttgtca gtttatcaaa taaaatgatt gagattcgac gagatggttt tcaaaataaa
     4321 ttgcaattaa atacaaatta tgaagaaata gataatttag caaatacgtt taatgagatg
     4381 atgagccaaa ttgaagaatc atttaatcaa caaagacaat ttgttgaaga tgcgtcacat
     4441 gaattacgaa caccattaca aattattcaa ggtcatttaa atttgattca gcgatgggga
     4501 aaaaaagacc cagcagtatt agaagaatcg ttaaatattt ctattgaaga aatgaatcgt
     4561 atcataaaat tagtcgaaga attacttgaa ttgactaaag gagatgtaaa tgacatttct
     4621 tctgaagcgc agaccgtgca tattaataat gaaattcgct cgcgaataca ctcattaaaa
     4681 caattgcatc ctgattatca atttgatacg gatctgacat ctaaaaatct agaaattaaa
     4741 atgaaacctc atcaattcga acaattattt ttaatcttta ttgataatgc aatcaaatat
     4801 gatgtgaaga ataagaaaat taaagttaag acaaggttaa aaaataagca aaaaataatt
     4861 gaaattacag atcatggaat tggtattcca gaggaagatc aagatttcat ttttgatcgc
     4921 ttttatcgag tggataaatc tcgttcaaga agtcaaggcg gtaatggact cggattatct
     4981 attgctcaaa aaatcattca attaaacgga ggatcgatta aaattaaaag tgaaattaac
     5041 aaaggaacaa cgtttaaaat catattttaa tcatgactga gacgtcaatc aaagtcatag
     5101 gatcaatttt ttaagtacac actagctgtg actaatgtat aagaacaact ataaaacaaa
     5161 taaacagtgg ttctttatca attctgttgt actcccaaaa tttacaataa aatacatcta
     5221 taaacctaga agaatcaacg cttttgttga ttcttctttt tagcagataa ataggtaaat
     5281 ctactttaac aaataactaa atagtgatat tattacattg taagcgtttc aacatttttg
     5341 tggagggtgt aaaatgacta acgaaagaaa agaagtttca gaggctcctg taaacttcgg
     5401 tgcgaattta ggtctaatgt tagatctata tgatgacttt ttacaagatc catcatctgt
     5461 accagaagat ttacaagtct tattcagcac aattaagaat gatgactcaa ttgtaccagc
     5521 tttaaaaagt acaagtagtc aaaatagcga cggcacaatt aagcgtgtca tgcgtttaat
     5581 tgataatatt cgccaatacg ggcatcttaa agccgatatt tatcctgtaa atcctccaaa
     5641 aaggaaacat gtacctaaat tagagattga agactttgat ttagatcaac agactttgga
     5701 aggtatatca gcaggaattg tttcagatca ctttgccgac atttatgata atgcttatga
     5761 agcaatttta agaatggaaa aacgttacaa aggaccaatt gcatttgagt atacacatat
     5821 taataacaat accgaacgtg gttggttaaa aagaagaatt gaaacgccat ataaagtaac
     5881 gttaaataat aacgaaaaaa gggcactatt caaacaatta gcgtatgttg aagggtttga
     5941 aaaatatctt cataaaaact tcgttggtgc aaagcgtttt tcaattgaag gggtagacgc
     6001 acttgtaccg atgttacaac gtactattac gattgctgcg aaagaaggta ttaaaaatat
     6061 acaaataggc atggctcacc gtggacgttt aaacgtttta acgcatgtct tagaaaaacc
     6121 gtacgaaatg atgatttcag aatttatgca tacagatcca atgaaattct tacctgaaga
     6181 tggtagcttg cagttaactg ctggatggac tggtgatgtg aaatatcacc ttggtggcat
     6241 taaaactact gattcatacg gtacaatgca gcgtattgca ctggctaaca atccaagtca
     6301 cttggaaatt gttgcacctg ttgttgaggg gcgtacgaga gcagcacaag atgatacaca
     6361 acgtgctggg gctccgacga ctgatcatca taaagcaatg ccaattatta tacatggcga
     6421 tgctgcttat cctggtcaag gaattaactt cgaaacaatg aacttaggaa acttgaaagg
     6481 ctattctacg ggtggttcat tgcatattat tactaacaat agaattggat ttactacaga
     6541 accaattgat gcacgttcaa caacttattc tacagatgtg gccaaaggtt atgatgtgcc
     6601 aatattccat gtcaatgctg atgacgttga agctactatt gaagcaattg atattgcaat
     6661 ggaatttaga aaagagtttc ataaagacgt cgttattgat ttagtaggtt atcgtcgttt
     6721 cggtcataac gaaatggatg aaccatcaat tactaatcca gttccttatc agaatattcg
     6781 caaacatgac tctgttgaat atgtgtttgg taaaaagctt gttaatgaag gtgtcatttc
     6841 agaagatgaa atgcattcat ttatagaaca agtccaaaag gaactaagac aagcacatga
     6901 taaaattaat aaagctgata aaatggataa tccagatatg gaaaaacctg cagaacttgc
     6961 attaccgtta caagcagacg aacaatcatt tacgtttgat catttgaaag aaataaatga
     7021 tgcattgtta acatatccgg atggctttaa cattttgaaa aagttaaaca aagttcttga
     7081 gaagcgtcat gagccgttta ataaagaaga tggtttagtt gattgggcac aagcagaaca
     7141 acttgcattt gcgacaattt tacaagatgg tacaccgatt cgcttaactg gtcaagatag
     7201 tgaacgtggt acattcagtc ataggcatgc cgtgttacat gatgagcaaa caggtgaaac
     7261 atatacacct ttacatcatg ttcctgatca aaaagcgaca tttgatatac acaattctcc
     7321 gctttcagaa gcagcagtag ttggttttga atacggctat aatgtggaaa acaaaaaaag
     7381 cttcaatatt tgggaagcgc aatatggtga ttttgcaaat atgtcacaaa tgatttttga
     7441 caacttctta ttcagttctc gctcaaaatg gggagaacgt tcaggattaa cattattctt
     7501 acctcatgca tatgagggtc aaggacctga acattcatca gcaagattag agcgattttt
     7561 acaattagct gctgaaaata attgcacagt tgtcaactta tctagttcaa gtaattattt
     7621 ccacttattg cgtgcacaag cagctagttt agattctgaa caaatgcgac cattggttgt
     7681 tatgtcacca aaaagcttat tgagaaataa aacagttgcc aaaccaattg atgagtttac
     7741 ttctggtgga tttgagccaa ttttgacaga atcatatcaa gcggataagg ttacaaaagt
     7801 tattttagca actggtaaaa tgttcattga tttaaaagaa gcattagcta aaaatccaga
     7861 cgaatcagtg ttactcgttg cgattgaaag attgtatcca ttcccagagg aagagattga
     7921 agcattacta gcacaattgc caaaccttga agaagtgtca tgggtacaag aagaacctaa
     7981 aaatcaaggt gcatggttat atgtctatcc atatgttaaa gtgctagttg cagataaata
     8041 tgatttaagt tatcatggca gaattcaaag ggctgctcca gctgaaggcg atggagaaat
     8101 tcataaactt gttcaaaata aaattataga aaatgcatta aaaaataact agggggaaat
     8161 aagtcatgcc agaggttaaa gttccagaat tagcagaatc tattacagaa ggtaccattg
     8221 cagaatggtt gaaaaacgta ggggatagcg tagaaaaagg tgaagctatt cttgaattag
     8281 aaactgataa agttaatgtc gaagttgtat ctgaagaagc aggtgtatta tctgaacaac
     8341 ttgcaagtga aggcgacact gtagaagttg gacaagcaat tgctatcatc ggcgaaggta
     8401 gtggcaatgc ttttaaagaa aatagtaacg acaatactcc acaacaaaat gaagaaacaa
     8461 ataataaaaa agaagaaaca acaaataatt cggtagataa agctgaagtc aatcaagcaa
     8521 atgatgacaa tcagcaacgt attaatgcta cgccttctgc gcgtcgatat gctcgtgaaa
     8581 atggtgtgaa tcttgctgaa gtaagtccga aaacaaatga tgtggttcgt aaagaagata
     8641 ttgataagaa acaacaggca ccggcatcaa cacaaacaac acaacaagca cctgcaaaag
     8701 aagagaaaaa atacaatcaa tatcctacaa aaccagtgat tcgtgaaaaa atgtcacgca
     8761 gaaagaaaac agctgccaaa aaattattag aggtatctaa taatacagct atgttaacaa
     8821 catttaacga agttgacatg acaaatgtta tggaattgcg taaacgtaag aaagaacaat
     8881 ttatgaaaga tcatgatggt actaaattag gatttatgtc attctttact aaagcttctg
     8941 tagcagcttt gaaaaagtat ccagaagtta atgcagaaat cgatggcgac gacatgatta
     9001 cgaaacaata ttatgatatt ggtgtagctg tttctacaga tgatggatta ttagtaccat
     9061 ttgtaagaga ttgtgataaa aagaattttg cagaaatcga agcagaaatt gctaatttag
     9121 cagttaaagc acgagagaaa aaacttggct tagatgatat ggttaatggt tcatttacga
     9181 ttacaaatgg cggtattttt ggatcaatga tgagtacgcc aattatcaat ggtaatcaag
     9241 ctgcaatctt aggcatgcat tcaattatta caagaccaat tgcgattgat caagatacaa
     9301 tcgaaaatcg tccaatgatg tatattgcat taagctatga tcatagaatt attgacggta
     9361 aagaagcagt tggattctta aaaacaatta aagaattaat tgaaaaccca gaagacttat
     9421 tattagaatc ttaatccaca acacaaaata gtttactatt cctaaaaacg taatcaaatc
     9481 aataacaaac agcagtaaga ttattttcta gtcga
//
LOCUS       DAHHYJ010000101         9471 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000101 DAHHYJ010000000
VERSION     DAHHYJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.101"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            128..526
                     /gene="nrdI"
                     /locus_tag="NBK22_001909"
     CDS             128..526
                     /gene="nrdI"
                     /locus_tag="NBK22_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCD2415092.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            489..2594
                     /gene="nrdE"
                     /locus_tag="NBK22_001910"
     CDS             489..2594
                     /gene="nrdE"
                     /locus_tag="NBK22_001910"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCD2415093.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            2712..3683
                     /gene="nrdF"
                     /locus_tag="NBK22_001911"
     CDS             2712..3683
                     /gene="nrdF"
                     /locus_tag="NBK22_001911"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCD2415094.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            3864..4019
                     /locus_tag="NBK22_001912"
     CDS             3864..4019
                     /locus_tag="NBK22_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415095.1"
                     /translation="MFYFTLSKQNTHSEKLNFSLIANINKIDTKLFERLLKLWSKNAT
                     INEKYYQ"
     gene            4058..5029
                     /locus_tag="NBK22_001913"
     CDS             4058..5029
                     /locus_tag="NBK22_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415096.1"
                     /translation="MKYLLKGNILLLLLILLTIISLFIGVSELSIKDLLHLTESQRNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSICGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            5016..5972
                     /locus_tag="NBK22_001914"
     CDS             5016..5972
                     /locus_tag="NBK22_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD2415097.1"
                     /translation="MRNNNKKIMLLIAVTLLISMLYLFVGIDFEIFEYQFSSRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVTFSAVLLVILLVITILLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            5969..6730
                     /locus_tag="NBK22_001915"
     CDS             5969..6730
                     /locus_tag="NBK22_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2415098.1"
                     /translation="MIQVENLTKTINNQMILEDISIDIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDNGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHDIVNDALDLLQLQDIRNRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMNIQIEHIRNQRICLYFKD"
     gene            6849..7877
                     /locus_tag="NBK22_001916"
     CDS             6849..7877
                     /locus_tag="NBK22_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2415099.1"
                     /translation="MKKTVLYLVLAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKNFNKT
                     VMYLLVNEGELSTFGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYVNKENPDV
                     ILAMDRGQAISGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELDKVVK"
     gene            complement(8194..8508)
                     /locus_tag="NBK22_001917"
     CDS             complement(8194..8508)
                     /locus_tag="NBK22_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415100.1"
                     /translation="MPKVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(8526..9449)
                     /locus_tag="NBK22_001918"
     CDS             complement(8526..9449)
                     /locus_tag="NBK22_001918"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCD2415101.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSIIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
BASE COUNT         3343 a         1350 c         1680 g         3098 t
ORIGIN      
        1 tgagaaaata agtagcttca ttatagttaa tacaatgctg agataaccat agtaaccatg
       61 ttgttaaagc attttttaat tggaatgact actttattta aaagggttga agaaagaagg
      121 tgatccaatg aaaataatat atttttcatt tactggaaat gtccgtcgtt ttattaagag
      181 aacagaactt gaaaatacgc ttgagattac agcagaaaat tgtatggaac cagttcatga
      241 accgtttatt atcgttactg gcactattgg atttggagaa gtaccagaac ccgttcaatc
      301 ttttttagaa gttaatcatc aatacatcag aggtgtggca gctagcggta atcgaaattg
      361 gggactaaat ttcgcaaaag cgggtcgcac gatatcagaa gagtataatg tccctttatt
      421 aatgaagttt gagttacatg gaaaaaacaa agacgttatt gaatttaaga acaaggtggg
      481 taattttaat gaaaaccatg gaagagaaaa agtacaatca tattgaatta aataatgagg
      541 tcactaaacg aagagaagat ggattcttta gtttagaaaa agaccaagaa gctttagtag
      601 cttatttaga agaagtaaaa gacaaaacaa tcttcttcga tactgaaatc gagcgtttac
      661 gttatttagt agacaacgat ttttatttca atgtgtttga tatttatagt gaagcggatc
      721 taattgaaat cactgattat gcaaaatcaa tcccgtttaa ttttgcaagt tatatgtcag
      781 ctagtaaatt tttcaaagat tacgctttga aaacaaatga taaaagtcaa tacttagaag
      841 actataatca acacgttgcc attgttgctt tatacctagc aaatggtaat aaagcacaag
      901 ctaaacaatt tatttctgct atggttgaac aaagatatca accagcgaca ccaacatttt
      961 taaacgcagg ccgtgcgcgt cgtggtgagc tagtgtcatg tttcttatta gaagtggatg
     1021 acagcttaaa ttcaattaac tttattgatt caactgcaaa acaattaagt aaaattgggg
     1081 gcggcgttgc aattaactta tctaaattgc gtgcacgtgg tgaagcaatt aaaggaatta
     1141 aaggcgtagc gaaaggcgtt ttacctattg ctaagtcact tgaaggtggc tttagctatg
     1201 cagatcaact tggtcaacgc cctggtgctg gtgctgtgta cttaaatatc ttccattatg
     1261 atgtagaaga atttttagat actaaaaaag taaatgcgga tgaagattta cgtttatcta
     1321 caatatcaac tggtttaatt gttccatcta aattcttcga tttagctaaa gaaggtaagg
     1381 acttttatat gtttgcacct catacagtta aagaagaata tggtgtgaca ttagacgata
     1441 tcgatttaga aaaatattat gatgacatgg ttgcaaaccc aaatgttgag aaaaagaaaa
     1501 agaatgcgcg tgaaatgttg aatttaattg cgcaaacaca attacaatca ggttatccat
     1561 atttaatgtt taaagataat gctaacagag tgcatccgaa ttcaaacatt ggacaaatta
     1621 aaatgagtaa cttatgtacg gaaattttcc aactacaaga aacttcaatt attaatgact
     1681 atggtattga agacgaaatt aaacgtgata tttcttgtaa cttgggctca ttaaatattg
     1741 ttaatgtaat ggaaagcgga aaattcagag attcagttca ctctggtatg gacgcattaa
     1801 ctgttgtgag tgatgtagca aatattcaaa atgcaccagg agttagaaaa gctaacagtg
     1861 aattacattc agttggtctt ggtgtgatga atttacacgg ttacctagca aaaaataaaa
     1921 ttggttatga gtcagaagaa gcaaaagatt ttgcaaatat cttctttatg atgatgaatt
     1981 tctactcaat cgaacgttca atggaaatcg ctaaagagcg tggtatcaaa tatcaagact
     2041 ttgaaaagtc tgattatgct aatggcaaat atttcgagtt ctatacaact caagaatttg
     2101 aacctcaatt cgaaaaagta cgtgaattat tcgatggtat ggctattcct acttctgagg
     2161 attggaagaa actacaacaa gatgttgaac aatatggttt atatcatgca tatagattag
     2221 caattgctcc aacacaaagt atttcttatg ttcaaaatgc aacaagttct gtaatgccaa
     2281 tcgttgacca aattgaacgt cgtacttatg gtaatgcgga aacattttac cctatgccat
     2341 tcttatcacc acaaacaatg tggtactaca aatcagcatt caatactgat cagatgaaat
     2401 taatcgattt aattgcgaca attcaaacgc atattgacca aggtatctca acgatccttt
     2461 atgttaattc tgaaatttct acacgtgagt tagcaagatt atatgtatat gcgcactata
     2521 aaggattaaa atcactttac tatactagaa ataaattatt aagtgtagaa gaatgtacaa
     2581 gttgttctat ctaacaatta aatgttgaaa atgacaaaca gctaatcatc tggtctgaat
     2641 tagcagatga ttagactgct atgtctgtat ttgtcaatta ttgagtaaca ttacaggagg
     2701 aaattatatt catgatagct gttaattgga acacacaaga agatatgacg aatatgtttt
     2761 ggagacaaaa tatatctcaa atgtgggttg aaacagaatt taaagtatca aaagacattg
     2821 caagttggaa gactttatct gaagctgaac aagacacatt taaaaaagca ttagctggtt
     2881 taacaggctt agatacacat caagcagatg atggcatgcc tttagttatg ctacatacga
     2941 ctgacttaag gaaaaaagca gtttattcat ttatggcgat gatggagcaa atacacgcga
     3001 aaagctattc acatattttc acaacactat taccatctag tgaaacaaac tacctattag
     3061 atgaatgggt tttagaggaa ccccatttaa aatataaatc tgataaaatt gttgctaatt
     3121 atcacaaact ttggggtaaa gaagcttcga tatacgacca atatatggcc agagttacga
     3181 gtgtattttt agaaacattc ttattcttct caggtttcta ttatccacta tatcttgctg
     3241 gtcaagggaa aatgacgaca tcaggtgaaa tcattcgtaa aattctttta gatgaatcta
     3301 ttcatggtgt atttaccggt ttagatgcac agcatttacg aaatgaacta tctgaaagtg
     3361 agaaacaaaa agcagatcaa gaaatgtata aattgctaaa tgacttgtat ttaaatgaag
     3421 agtcatacac aaaaatgtta tacgatgatc ttggaatcac tgaagatgtg ctaaactatg
     3481 ttaaatataa tggaaacaaa gcactttcaa acttaggctt tgaaccttat tttgaggaac
     3541 gtgaatttaa cccaatcatt gagaatgcct tagatacaac aactaaaaac catgacttct
     3601 tctcagtaaa aggtgatggt tatgtattag cattaaacgt agaagcatta caagatgatg
     3661 actttgtatt tgacaacaaa taacaattaa attaaaagac cttcacatgt aaagggaaat
     3721 agcgattcgt ttcgtcttgc ctcctacatg ttgaaggtct tttctctatg cttaaaagcg
     3781 tatgcatatt ttgaaaaagt aaaaattgta tttaatgatg tcaatgaggc tagaaagtat
     3841 acaagatatt gcattacaaa agggtgttct attttacttt aagcaaacaa aatacacatt
     3901 ccgaaaaatt aaatttcagt ttaattgcaa atatcaataa aattgacact aaattatttg
     3961 aaaggctatt gaaattatgg tcaaaaaacg ctactattaa tgagaaatat tatcaatgat
     4021 aatgattatc attaatttaa agggagaaaa atttgtaatg aagtatttat taaagggaaa
     4081 tattttgctt ctattactaa tattgttgac aattatttcg ttgttcatag gtgtgagtga
     4141 actatcaatt aaagatttac tacatttaac tgagtcacag cggaatattt tattctcaag
     4201 ccgaatacca aggacgatga gtattttaat tgctggaagt tcgttggctt tagcaggctt
     4261 gataatgcaa caaatgatgc aaaataagtt tgttagtccg actacagctg gaacgatgga
     4321 atgggctaaa ctaggtattt taattgcttt attgttcttt ccaaccggtc atattttatt
     4381 aaaactagta tttgctgtta tttgcagtat ttgcggtacg tttttatttg ttaaaatcat
     4441 tgattttata aaagtgaaag atgtcatttt tgtaccgctt ttaggaatta tgatgggtgg
     4501 gattgttgca agtttcacaa ccttcatctc attgcgcacg aatgctgttc aaagcattgg
     4561 taactggctt aacgggaact ttgccattat cacaagtgga cgctatgaaa ttttatattt
     4621 aagtattcct cttttagcat tgacatatct ttttgctaat catttcacga ttgtaggaat
     4681 gggtaaagac tttactaata atttaggttt gagttacgaa aaattaatta acatcgcatt
     4741 gtttattact gcaactatta cagcattggt agtggtgact gttggaacat taccgttctt
     4801 aggactagta ataccaaata ttatttcaat ttatcgaggt gatcatttga aaaatgctat
     4861 ccctcatacg atgatgttag gtgccatctt tgtattattt tctgatatag ttggcagaat
     4921 tgttgtttat ccatatgaaa taaatattgg tttaacaata ggtgtatttg gaacaatcat
     4981 tttccttatc ttgcttatga aaggtaggaa aaattatgcg caacaataat aaaaaaataa
     5041 tgcttttaat tgcagtaacg ttattaatta gtatgctgta cttatttgta ggtattgatt
     5101 ttgaaatatt tgaatatcaa ttttcaagtc gtttaagaaa gttcatatta attattttag
     5161 taggtgctgc cattgcaact tcagtggtga tttttcaagc gattacaaat aaccgtctat
     5221 tgacaccatc aataatgggg ttagatgcag tttatttatt tatcaaagta ttgccagtct
     5281 ttttatttgg aattcaatcg gtatgggtta ctaatgtata tttgaacttt atattaacac
     5341 ttataacgat ggtgttattc gcactaatcc tattccaagg tatctttaaa attggacatt
     5401 tttcaattta ttttatctta cttattggtg tccttttagg aacatttttt agaagcataa
     5461 caggttttat tcaactgatt atggatcctg agtcattttt agcaatacaa agtagtatgt
     5521 ttgctaattt taatgcttct aattcgaatt tagttacttt ctcagcagtg ctattagtaa
     5581 tcttattagt cattacaatt ttactattgc cttatttaga tgtattgctt ttaggtcgtg
     5641 ctgaagcaat taatcttggg atatcgtatg aaaaattaac gcgaattcta cttgtaatag
     5701 tctcagtttt agtttctgtg tcaactgcat tagtaggacc aattacattt ttaggtttat
     5761 taactgtaaa tctagcgcat gaactaatga agacgtatga acataagtat attttaattg
     5821 cgacaatttg cttgagttgg attagtttat ttagtgcgca atgggtagtt gaaaatgtgt
     5881 ttgaagctac gacagaaatg agtatactta ttgatttgat tggtggaagt tatttcattt
     5941 atctattagt tagaaggaga aatgcgcaat gattcaagtt gaaaatttaa ctaaaactat
     6001 aaataatcaa atgatattgg aagatattag catagatatc gaaaaaggta aattgacttc
     6061 tttaattgga cctaatggtg cgggtaagag tactttactt tcagcgatat gtaggttaat
     6121 tcgttttgat aacggtgaag tgaaaataga tggacagctc atgtctgatt ataaaaataa
     6181 tgacttgtcg aaaaaaatat ctatattaaa acaaacaaac catactgaaa tgaatattac
     6241 ggtagagcag ttggtaaact ttggacgatt cccttattct aaaggtcgtt tgacgaaaga
     6301 ggatcatgat attgtcaatg atgcgctaga tttgttgcaa ctacaagata tcagaaatcg
     6361 taatattaag tcattatctg gtggacaacg tcagcgtgca tacattgcaa tgacaatagc
     6421 acaagatact gaatatattt tgctagatga accattaaat aatttagata tgaagcatgc
     6481 tgttcaaatt atgcaaacgt taaaaatgtt agcgcataaa atgaataaag cgattgtcat
     6541 tgtgttacat gatattaact ttgcgtcctg ttattcagat cagattgtag cattgaaaaa
     6601 cggacaacta gttaagtcag atttgaaaga taatgtcatt caaagtagtg ttttaagtga
     6661 tttatatgac atgaatattc aaattgaaca tataagaaat caaaggattt gtttatattt
     6721 taaggattga taatttggag acactttaaa ggggtgatgc gccaattaaa gaagggttaa
     6781 acgtaaagca tttatttata tttcacatca agcacacaga ttaagccaaa agaggagaat
     6841 attatattat gaagaaaaca gtcttatatt tagtattagc agtaatgttt ttattagcgg
     6901 catgcggtaa caattctgat aaagaacaat caaaatcaga aactaaaggt tctaaagata
     6961 cagtaaaaat tgaaaataac tataaaatgc gtggcgagaa aaaagatggt agtgacgcta
     7021 aaaaagttaa agaaactgtt gaagtaccaa aaaatcctaa aaatgcagtt gtgttagact
     7081 atggcgcatt agatgtaatg aaagaaatgg gcttatcaga taaagtaaaa gcattaccta
     7141 aaggggaagg cggtaagtca ttaccgaatt tcttagaatc atttaaagat gataaatata
     7201 caaacgttgg taatttaaaa gaagtgaatt ttgataaaat tgctgcgacg aaacccgaag
     7261 taatctttat ctctggacgt acagctaatc aaaagaattt agatgaattc aaaaaagctg
     7321 cacctaaagc gaaaattgtt tatgttggtg cagatgaaaa gaacttaatt ggttcaatga
     7381 aacaaaacac tgaaaatatc ggtaaaattt acgataaaga agataaagct aaagaattaa
     7441 ataaagattt agataacaaa attgcttcaa tgaaagataa aacgaaaaac ttcaataaaa
     7501 ctgttatgta tttactagtt aacgaaggtg aattatcaac atttggacct aaaggtcgtt
     7561 ttggtggatt agtttacgat acattaggat tcaatgcagt tgataaaaaa gtaagtaata
     7621 gcaatcatgg acaaaatgtt tctaacgaat atgttaataa agaaaatcca gatgttattt
     7681 tagcgatgga tagaggtcaa gcgataagtg gtaaatcaac tgcgaaacaa gcattaaata
     7741 atcctgtatt aaaaaatgtt aaagcaatta aagaagacaa agtatataat ttagatccta
     7801 aattatggta ctttgcagct ggatcaacta caactacaat taaacaaatt gaggaacttg
     7861 ataaagttgt aaaataattt taaaagaggg gaacaatggt taaaggtctt aatcattgct
     7921 cccctctttt ctttaaaaaa ggaaatctgg gacgtcaatc aatgtcctag actctaaaat
     7981 gttttgttgt cagtcgttgg ttgaatgaac atgtacttgt aacaagttca tttcaatacc
     8041 agtgggctcc aaacatagag aaatttgatt ttaaatttct actgacaatg caagttggcg
     8101 gggcccaaac atagagaatt tcaaaaagga attctacaga agtggtgctt tatcatgtct
     8161 gacccactcc ctataatgtt ttgactatgt tgtttaaatt tcaaaataaa tatgatagtg
     8221 atatttacag cgattgttaa accgagattg gcaatttgga caacgctcta ccatcatata
     8281 ttcattgatt gttaattcgt gtttgcatac accgcataag attgcttttt cgttaaatga
     8341 aggctcagac caacgcttaa tggcgtgctt ttcaaactca ttatggcact tatagcatgg
     8401 atagtattta ttacaacatt taaatttaat agcaataata tcttcttcgg taaaataatg
     8461 gcgacagcgt gtttcagtat cgattaatga accataaact ttaggcatag acaaagctcc
     8521 ttaacttacg attcctttgg atgttcacca ataatgcgaa cttcacgatt taattcaatg
     8581 ccaaattttt ctttgacggt cttttgtaca taatgaataa ggttttcata atctgtagca
     8641 gttccattgt ctacatttac cataaaacca gcgtgtttag ttgaaacttc aacgccgcca
     8701 atacggtgac cttgcaaatt agaatcttgt atcaatttac ctgcaaaatg accaggcggt
     8761 ctttggaata cactaccaca tgaaggatac tctaaaggtt gtttagattc tctacgttct
     8821 gttaaatcat ccattttagc ttgtatttca gtcattttac caggagctaa agtaaatgca
     8881 gcttctaata caactaagtg ttctttttga ataatgctat tacgataatc taactctaat
     8941 tcttttgttg taagtttaat tatcgagcct tgttcgttta cgcaaagcgc atagtctata
     9001 caatctttaa cttcgccacc ataagcgcca gcattcatat acactgcacc accaattgaa
     9061 cctggaatac cgcatgcaaa ttcaaggcca gtaagtgcgt aatcacgagc aacacgtgag
     9121 acatcaataa ttgcagcgcc gctaccggct attatcgcat catcagatac ttcgatatga
     9181 tctagtgata ataaactaat tacaatgcca cgaataccac cttcacggat aataatattt
     9241 gagccatttc ctaaatatgt aacaggaatc tcattttgat aggcatattt aacaactgct
     9301 tgtacttctt catttttagt aggggtaatg taaaagtcgg cattaccacc tgttttagta
     9361 taagtgtatc gttttaaagg ttcatcaact ttaatttttt catttgggat aagttgttgt
     9421 aaagcttgat agatgtcttt atttatcact tctcagtaca tcctttctca t
//
LOCUS       DAHHYJ010000102         9422 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000102 DAHHYJ010000000
VERSION     DAHHYJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.102"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..628)
                     /locus_tag="NBK22_001919"
     CDS             complement(<1..628)
                     /locus_tag="NBK22_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCD2415102.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRK
                     RQTILLYAVTGAGKTEMMFQGIQYARIQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSRQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKNLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENIIKLPARFHKKSLPVPKYRYFKLNN"
     gene            complement(992..1858)
                     /gene="fakB1"
                     /locus_tag="NBK22_001920"
     CDS             complement(992..1858)
                     /gene="fakB1"
                     /locus_tag="NBK22_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCD2415103.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQDDCPSAYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            2002..2643
                     /locus_tag="NBK22_001921"
     CDS             2002..2643
                     /locus_tag="NBK22_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCD2415104.1"
                     /translation="MTQNIITVKKEHIIENVISKSRFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDIGRVELREAVPCIVTLNYDQTGKFEYELASTHFI
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            complement(2816..3871)
                     /locus_tag="NBK22_001922"
     CDS             complement(2816..3871)
                     /locus_tag="NBK22_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCD2415105.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFVLIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKEE"
     gene            4189..5259
                     /locus_tag="NBK22_001923"
     CDS             4189..5259
                     /locus_tag="NBK22_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCD2415106.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            5455..6216
                     /locus_tag="NBK22_001924"
     CDS             5455..6216
                     /locus_tag="NBK22_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCD2415107.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            6234..6728
                     /locus_tag="NBK22_001925"
     CDS             6234..6728
                     /locus_tag="NBK22_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD2415108.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            6742..7968
                     /gene="pepT"
                     /locus_tag="NBK22_001926"
     CDS             6742..7968
                     /gene="pepT"
                     /locus_tag="NBK22_001926"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCD2415109.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKPQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAVITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(8150..9274)
                     /locus_tag="NBK22_001927"
     CDS             complement(8150..9274)
                     /locus_tag="NBK22_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD2415110.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDVHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPETYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
BASE COUNT         3167 a         1625 c         1436 g         3194 t
ORIGIN      
        1 tattattaag tttgaaataa cgatatttag gaactggaag tgatttttta tgaaagcgag
       61 ctggcaattt aattatattt tcgtggggaa tctctgacag aagttgtttc ggtggtgttg
      121 ctgtcatata aattgttgca tgttcaactt tagaagatga cttcaatgct tgttgtagat
      181 ttttatccat tgataaagga aaggcatcga cttcatcaat aaaaatagta tcaaagtgct
      241 gtttgaatcg gtaaagttga tgcactgtgc atacaacaaa atgcccttca aattgttgtc
      301 ttgattgctg gtgtagtatg tctatatctt cattaagaaa tgcgtcttta atacgtttac
      361 taatttctac aacaacatct acacgtggtg acacaatagc tatattatct ccctgtattc
      421 ttgcatattg aatgccttga aacatcattt ctgtcttacc tgcacctgtt acggcatata
      481 acaaaatcgt ttgtctcttt ctaatggctc gaacaatatg ttctgaggca taagactgct
      541 gttccgacaa ttcaaatgag agatgataat atgcttgtga tgaactttgc gaactctctg
      601 ttattttata tcttgttaca ttatccattc gacccagtga tatacaattt ctgcaatata
      661 caattttctt ggaatgtact gaggaatcat actgataaaa atgctttgga tttttacttt
      721 cacattgaac acaataatag tttgaatttt gtttgatgac acctgttact tcttttttaa
      781 ttttttcatt agtaagtgaa gtatgaggat gaattaattt cccgtaatgt ttaatgctta
      841 aaacctcatt tcaacgtaaa aaagagccta atattataat gattttctta tatctttagc
      901 attttggctt tcaaatgatg aacatttaaa gccatgtata cgagagataa tttccaatta
      961 caatatagac tcttttatta aaattttata attatgttaa tcttattttt ctgccaacat
     1021 agcctaaacc taatccacca gaacctaaat gtgctgcaac aactggacca aactcagagt
     1081 atgctacttg ataagctgaa ggacaatcat cttgtaactt tttgtataac gcttgaccat
     1141 cttcgaaatg atctccattt atgacaaata aagttacttc ttcaaagtct tttacaatat
     1201 ctaatacttt cttttctaat gtttgaatgg cacgcttttt agtacgaact ttttcttctg
     1261 gtataatctt gccatcttca aacttaagaa ctggcttcat tttcaataat gttccaaccc
     1321 atgcttgagc accagtaatt cgaccacttt tttgtaaatt ctttaagtca tcaacaatta
     1381 aatatgcacc tgtgtgttca cgcatattag ttaaatcatc aataatttgc tgtggttcgt
     1441 atccttcttc aaccatttca atagcacgta atacatagca tccttcaatc attgctgcaa
     1501 gcttactatc aaatgcatgt acattaacac cttcaaccat ttcccctgct tgataactag
     1561 attgataact tccactaatt ccacttgata agcaaatgac aatgatatct gtgtaaccct
     1621 gatctcttag catttcatat ttagtaatcc attcgccaat tgctggttgg cttgttgttg
     1681 gaatcgtttg agacgatgcc attttattat aaaattcttc aattgctatt tcattacttt
     1741 ctgtaaagtt cttcccatca tcaaaagtca cacttaatgg cgctatttga atattatatt
     1801 tatcgattaa gtcctgcgac agataacttg tagaatcggt catcacagca attttcatag
     1861 tcgtcctcct aaaatatact catagcaatg atacaatatt tcagttaaaa agaaaatggc
     1921 aatcaaacga tttatagtca tgtcattttc agtgtataat gaagattaaa taatcttaag
     1981 cttatgaatt aaggagttat tatgacacaa aatatcatta ctgttaaaaa agaacacatt
     2041 attgaaaatg tgattagtaa atcaagattc attgctcata tcaaacctgt tcaaaatgaa
     2101 gatgaagcta aagcatttat tgctgcaata aaaaaagaac ataaagatgc cactcataat
     2161 tgttctgcat atactattgg acctgaaatg aatattcaaa aagcgaatga tgatggtgaa
     2221 ccgacaggaa ctgccggtgt cccaatgtta gacatattaa aaaagctcga tgttcataat
     2281 gcgtgtgtcg tagtaacaag atattttggt ggtattaaat taggtggtgg cggtttaatt
     2341 cgcgcctata gcggtgctgt cagagacgtc atttatgata tcggacgtgt cgaactacga
     2401 gaagcagtac catgtatagt aacactgaat tatgatcaaa ctggtaaatt tgaatatgaa
     2461 ttagcttcaa ctcattttat attaagaaac caaacttaca ctgataaggt aagctactat
     2521 atcgatgtcg ttaaaagtga ctatgaagcg tttattaact ttttaaatca aatgactgct
     2581 ggaaatttcg atttgactga agaaactcca aagcagttac ctttcgatat accaactgaa
     2641 taaaaaataa aaagcactat cataagacgg taaatatcaa ttgcgtatta ttaaacgaca
     2701 attgaagtac acgtttatgg cagtgctttt ttatatttga aatttaatac ataaaaaatt
     2761 gtaaagagta aagccatata atacaaatta tcccatacag ctatgctttc attccctatt
     2821 cctctttatg agacgatttc cgactaatta aatttaatat tggtcggtag ttgttatcta
     2881 ttaatcctgt aaattcaaca attaactcaa tactaaatat gattaataca aacattaata
     2941 caacacctaa tggtggcgat acatacaaaa taatgctcga aagactaaat aaaatagaga
     3001 ttgaatagat taataatact gtttgtctat gtgtgtagcc taaagctaat agtttatgat
     3061 gcaaatgcga tttatcagct tgcattatat gctgcccttt tttcacacgt cgaatcattg
     3121 cgaacaaagt atcaatgaat ggaactgcta agataacaat tgggaagaac aatgcaataa
     3181 ttgtaatatt tttgaatccg agtaaagaaa ggaatccgat gataaatcca atcattaaag
     3241 ccccactatc acctaaaaat attttggcag gatggaaatt gtaaaataaa aacccaatta
     3301 aagagcctaa taaaacacaa caaatcatcg ttatgaaaat attagcttgt aaaattgcaa
     3361 tgaatcctat tgtaatgagt ccgattgcag aaacacccga cgccaaacca tcgagtccat
     3421 caattaagtt aattgcattt gtaatagcaa caatccaaat cacagtaatt ggaatactaa
     3481 gaaatccaaa atgaatcgtt gtacccattg gcaacgaaat aaaatcaata gtcacaccat
     3541 aaaaagcaac tactaaggca gcggcaattt gaccagccaa ttttatatac ggtttcaaat
     3601 cgtagatatc atctacaagc ccaagtacgt acataataat cgcaccaata ataagtggtt
     3661 tgatttctgt ttcaatagga tgaccaatcc aaatacctat taaaaatgaa aagagaatca
     3721 ctgtaccacc cataactgaa ataggtttag tgtgtacttt tctaaaattt ggtttatcaa
     3781 ctaaatttaa tcttttcgat attgcaataa caattggtgt tatcgtcaaa ctgacaatca
     3841 ttgttactgc aactagtaat aatgtaacca tttattcacc ttcatcgata ttaattgttt
     3901 ttaacgttat atttatttaa tattgtcttt atagaatgat atatctattt tctacgtata
     3961 tcttatagct ttattataaa ttaattcgct caatcaatga tatcatattt acaactatac
     4021 gattcatttc ttatatatcg cctattattc attttttcat catatcattt aaggcaacat
     4081 tttaacatca aagtctatat catacattaa ttttatatgt aaactaatga caaattgtaa
     4141 gttttcactt acaatagcta gtggtcaatt aaaggagtcg attcagacat gttcgaagca
     4201 tttatataca atatatctgt tatagtcgct ggaatctatt tattccatcg cttacagtat
     4261 tcagaaaaca aacgtatggt tttttctaag gcttatgtca ctgttttaat gacaatcgta
     4321 tcattattat tatctgtcta ccctatccct tatcgtgaag attatttaat tcacttaaca
     4381 ttcgttcctt tattgttttt aggtagattt acaaatatgg tctatacatt atctgccaca
     4441 gtaattgtag caattgttga aattgtagta tttaacaact cgattatgta cggtgttacg
     4501 ttgattgtta ttgccgcagt aacaagtgct attggaccat ttttaaaaca aaatgatgtg
     4561 ctatcattat taatacttaa tgttgttaca ataatcattt tatttggtgt cgcgttagtg
     4621 agtcctattt acacactatc ggaagttatc attttaatac caatttcatt aattatcact
     4681 ttagcttcag cgataacatt tgtcgatatt tggcatttct tctcactcgt aaatcgttat
     4741 gaaaacgaag ataaatatga ttacttaaca ggcttaggta atgtaaaaga atttgataga
     4801 catttaaatg aaatttcacg aaaagctgaa aaagaacatc aaagtatcgc attattatta
     4861 atcgatatcg atggatttaa agatgtcaat gatacctact cacacaaatc aggtgatgct
     4921 gtattaaaac aaatgtctca attacttaaa aactatgtgc cgaatcaatt taaaattttt
     4981 agaaatggtg gcgaagagtt ctcagttgtc attcacaatt attcacttga tcaaagtgtt
     5041 aaattggctg aaaatattcg ttcgggtgtt gaaaaatcat cattccattt accaaacaaa
     5101 gaagttatta aattgtcagt gtctatcggt gttggttatt taacagatga cgatccaaaa
     5161 tcacagcgta aagtgtttaa agatgcagac gatatggtgc atgtagccaa aaatcaaggg
     5221 cgaaacaaag taatgtttaa ccctattatc aatttataac aacgacaaaa ctgttaatcc
     5281 tttgctatta ttaaatcata gtataatagc aaagattaac agtttttctt atttaaattt
     5341 gaaccataca agttcatcat actaacacaa agtctcacta ttgaaagttg taacaacaag
     5401 ttgtaaactt caattgttca aataaataac acatatcatt ggaggtgaat tgatatgcca
     5461 gattcaatca caattataga tgaaaacaaa gtgattgatg ttgtattaat tgcaggtaga
     5521 attttacttg aatcaggtgc tgaaacatat cgagttgaag atacaatgaa ccgtatcgca
     5581 catagttatg gtcttcataa tacatatagt tttgtcagtt caactgcaat tattttttca
     5641 ttaaacgatc gaacaagtac aagattaatt cgtgtacaag agcgtacaac agatttagag
     5701 aaaatcgctt taacgaatag tctttcacgt aaaatatcaa ataaagaact cacaattgat
     5761 gaagctaaat ctgaatttat tcatttacag catgcatcat tacaatattc ttttttaacg
     5821 aatttctttg cagctgccat tgcatgtggc ttcttcctat ttatgtttgg tggtgtcgca
     5881 tcagattgtt ggattgcagt cattgctggc ggatccgcat ttttaacatt cagctttgtg
     5941 caacgctata tacaaattaa atttttctca gagtttgttg cagccgctgt ggtcatatcc
     6001 attgcagcca catttactaa attaggcatt gcaaccaatc aagatatcat tactatagcc
     6061 agtgtcatgc cacttgtccc tggtatatta attaccaatg ccattcgaga tttattagcg
     6121 ggtgaattac ttgcaggtat gtctcgtggt gttgaagctg cattaacagc attcgcaatc
     6181 ggtgctggtg tcgcaatcgt tttattaatc atttaaggaa ggactgttaa agcatgctat
     6241 tttatttatt tcattttaca atcagcttta tatcaacagt acttttctct atcattttca
     6301 atgcacccaa acgcctctta gtagcatgtg gatttgtggg tgccattgca tggacgattt
     6361 accaattaac ggtagattta gagtttggaa aagttggcgc ttcatttttg ggaagcttaa
     6421 ttttaggctt aatgagtcat actatgagcc gcagatataa acgaccggta attatattca
     6481 tagtgccagg cattatacca ttagtacctg gtggtgcagc ttatcaagcg actcgttttt
     6541 tagtatcaaa tgattataca agtgctgtaa atacattttt agaagttaca ctgatttcag
     6601 gtgcgattgc tttcggtata ttagtttctg aaattctata ttacctatac acacgtatca
     6661 aacaactgta tggtaaaatc aaaggtaaga catataaaaa atcttacaac atgaataata
     6721 gagtttaagg ggtcgaatat aatgaagaac caattgatag atagattaac aagatatacg
     6781 acaattgata cgcaatctga tccaaaatct acaacaacac cttcaactga aaagcaatgg
     6841 gatttgttac atttattaga aaaagaatta caacaattag gattgccaac tgatttagat
     6901 gaaaatggct acttatttgc tacattagaa agtaacatcg atgtcgacgt cccaacagtt
     6961 ggatttttgg cgcatgttga tacatcacct gatttcaatg cttcaaatgt taaaccgcaa
     7021 attattgaaa actatgatgg taagccatac aaactaggca atactaaacg tgtcttagat
     7081 cctaaagtat ttccagaact taacagttta gttggtcata cattaatggt aactgatggt
     7141 acatcgttac taggtgcaga cgataaagcg ggtattgtgg aaattatgga agctatttgt
     7201 tatttacaag aacatccaga aattaaacat ggtaccattc gcattggatt tacaccagac
     7261 gaagaaatcg gtcgtggtcc acataaattt gatgttgacc gcttcaatgc tgattttgct
     7321 tatactatgg atggtagtca atatggagaa ttacaatatg aaagctttaa cgctgctgaa
     7381 gcggttatta catgccacgg tgtaaatgtt catcctggtt cagctaaaaa tgcaatggta
     7441 aacgcaatac gtttaggtga acaattcgat agtttgctac ctgatagtga agttccggag
     7501 cgaacagaag gatacgaagg cttttatcac ttaatgaact ttgaaggaac tgttgaaaaa
     7561 gcaactttgc aatacattat tcgtgatcat gataaaaaac aattcgaatt gcgtaagaaa
     7621 cgtattttag aaatacgtga cgatatcaat gcccattttg aaaattatcc agttaaagtt
     7681 gatatatcgg atcaatattt caatatggca gaaaaaatat taccattgcc tcatattatt
     7741 gatataccta aacgtgtctt tgccaaatta gatattccag caaacactga acctattcgc
     7801 ggtggtacag atggttcaca attgtcattt atggggttac caactccaaa catctttaca
     7861 ggttgcggta acttccacgg cccatatgaa tatgcatcca ttgatgttat ggaaaaagca
     7921 gtacaagtaa tcattggaat tgtagaagat atcgctgaaa atcactaaaa accaacgatt
     7981 atataccaac tcaggcttaa ttaataaaaa aatcacgcta acattcagtg caatgatagc
     8041 gtgatttttc atatttttta cgtatacttt cgatcttcta attcgttttg ctaaccatca
     8101 taaatgttat taaccaacat gacagcatca ccaatttagt ccttctttac taaacctcaa
     8161 cattaaaatg tgttttcaac agttttaaag actgaaccgt ataatgccta atttgtaacc
     8221 ccattttaaa gtcagtataa gtttctggca tatcgataaa tgtattaaac attgctgtaa
     8281 ccccttgtga ttcaaataaa tcaaactttt cagcagttgc acaaattgca atggcaacct
     8341 tttgatgttt atgacaaagt tctgcaatac gcaatgttgt cgtttctagc aactgatcat
     8401 tttcatttaa cccttctcca aaaataatta aatccgcttg ttcaactaaa ttttctaaat
     8461 gtgttagttg gtctactaat gcatgactgg ttaatatttc agcttgatac agtccattca
     8521 agactgctgc aattccacca ccagcaccac cacgttcaac tggaccaatt gcaattttca
     8581 attcactttt aaataactca ctaaaatacc aaattaaatt atcgatttct gctgcttgat
     8641 tatgattcaa ctgatgcaca tcataagttt gcatgatttc actttgctta ccatataatc
     8701 gacttgaaaa atccgacatt acttgaattc ttgctgtttc cattttaggg tgtaagttcg
     8761 acatatccat acgacgaata tatttaatta caccagcacc ttgtctcata tctacgacac
     8821 gcccttcgtc atcatagaat tgagcaccta atgcttgtaa catacctgca ccagcatcaa
     8881 aactatcaat cccacctagt gaaattacaa catgttttgc gtcgttatct aatgcatgtt
     8941 taatcatttc tcctaaacca taacttgttc gttcaacaat tggtttttta ccttttaaaa
     9001 ataaattccc ctcgataacg gtcatccctg tatcagtttg tccgtaaaca ccttcaactt
     9061 cattcatatc tgcatcatgt actggtatac gatacttttg cccagattgc cataaaaata
     9121 cagaatctaa taattcatgt cttccattaa acaatggtac ttgaactaca tctgcagttt
     9181 caatttggct tgcaactgcc tcttcaacat atctattagc ttgataactc gaaataattc
     9241 catgaaactc atccatggct actaatactt tcataacctt gaccacctta ctttattatt
     9301 tgttgtgata cgttttttat tcataaaaga ttaatcttaa attttagatt ttatctcgtg
     9361 attatatgcg aacatttaac atgacatgat tattaattat tccagtaaat aacagtatgc
     9421 tt
//
LOCUS       DAHHYJ010000103         9322 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000103 DAHHYJ010000000
VERSION     DAHHYJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.103"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            222..1298
                     /locus_tag="NBK22_001928"
     CDS             222..1298
                     /locus_tag="NBK22_001928"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCD2415111.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            1548..2231
                     /locus_tag="NBK22_001929"
     CDS             1548..2231
                     /locus_tag="NBK22_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD2415112.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNATSLFDLIISTEADAYEKPNPKV
                     LSPLFEQNNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2347..3009)
                     /locus_tag="NBK22_001930"
     CDS             complement(2347..3009)
                     /locus_tag="NBK22_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD2415113.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMINKIARIIQTYRQVD
                     TR"
     gene            complement(3002..3619)
                     /locus_tag="NBK22_001931"
     CDS             complement(3002..3619)
                     /locus_tag="NBK22_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD2415114.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            3686..4156
                     /locus_tag="NBK22_001932"
     CDS             3686..4156
                     /locus_tag="NBK22_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCD2415115.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            4303..5172
                     /locus_tag="NBK22_001933"
     CDS             4303..5172
                     /locus_tag="NBK22_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2415116.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKEVMAIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            5193..5759
                     /locus_tag="NBK22_001934"
     CDS             5193..5759
                     /locus_tag="NBK22_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD2415117.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
     gene            6186..>9322
                     /gene="sdrD"
                     /locus_tag="NBK22_001935"
     CDS             6186..>9322
                     /gene="sdrD"
                     /locus_tag="NBK22_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCD2415118.1"
                     /translation="MLNRENKTAIRRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNAETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADNSKTIVPAQDYLS
                     LKSQITVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVKMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVSRDNNSIGSAFTETVSHAGNAEDPGYYIQTVYVNPSEKTLTNAKLKVEAYHKD
                     YPDNVGQINKNVTKIKIYQAPKDYVLNKGYDVNTNQLIDVTEQFKDKITYGTNDSVNV
                     DFGSINNSYVVMVDTKFEFTTSESPTLVQMATLTSDGNRSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGIQELGEVGVKGVTVVAYDNKTNKEVGRTITDEKGGYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLDNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLL"
BASE COUNT         3585 a         1257 c         1645 g         2835 t
ORIGIN      
        1 gttcatttta aagtaaagag ttttttgtat gtaaagtata ttagtaaaac acagattact
       61 tgtctctgtg aaattaatac gtttatacta aaagatatag tctttctaga aatctgttaa
      121 ttaattttga atttttagaa aatttgttga acagcaaaat atggattgtt ataatttaag
      181 ttaaacaaat tcttatacaa tattattagg aggcaatcac catgtcacaa gcagttaaag
      241 ttgaacgacg agaaacatta aaacaaaaac caaatacatc tcaactaggt tttggtaaat
      301 attttactga ttatatgttg agttatgatt atgatgcaga taaaggatgg catgatttga
      361 agatagtacc ttatggtcct attgaaattt cacctgctgc acaaggtgtt cattatggtc
      421 aatcggtatt cgaaggatta aaagcatata aaagagatgg ggaagttgca cttttccgtc
      481 ctgaagaaaa ttttaagcgt cttaataact cgttagcacg attagaaatg cctcaagtag
      541 acgaagcaga attgttagag gggctaaaac aattagttga tatagaaaga gattggattc
      601 ctgaagggga aggtcaatca ttatatattc gtccatttgt ttttgcaaca gaaggggcac
      661 ttggcgttgg tgcatcacat cagtataaat tattaattat tttatctcct tcaggtgcat
      721 attatggtgg tgagacttta aaaccaacta aaatctatgt agaagatgaa tatgtgcgtg
      781 ctgttcgtgg cggtgtaggc tttgcaaaag ttgcaggtaa ctatgcggca agtttattag
      841 cacaaacaaa tgcaaataaa ttaggttatg accaagtatt atggcttgat ggtgttgaac
      901 agaaatatat cgaagaagtt ggtagcatga atattttctt cgttgaaaat ggaaaagtaa
      961 ttacaccaga gttgaatggc agtattttac ctggtattac acgtaaatct attatcgaat
     1021 tagctaaaaa cttaggatat gaagtcgaag agcgccgcgt ttcaatcgat gaattattcg
     1081 aatcatatga taaaggtgag ttaacagaag tatttggtag tggtactgca gcagttattt
     1141 cacctgtggg tacattgaga tacgaagatc gtgaaatcgt tattaataat aatgagactg
     1201 gtgaaattac tcaaaaatta tacgacgtct atactggtat tcaaaatggt actttagaag
     1261 ataaaaatgg ttggagagtc gttgtaccaa aatattaata aaaattgaat ataatcatga
     1321 aaacaatatg taaaagttaa tcatgactaa aatccctttc aatgaagcga agcatggtaa
     1381 taaattaagt ttacaatgtt tattgttaag tattgaaggg gatttcactt attattatat
     1441 ttaattcaat atttaaaaag aacaatacta cgagttcatt ttcaggggat aacgaatatc
     1501 atagaatcag cataatacga aataaataaa taggagtatt gatatcaatg gaatggatat
     1561 tatttgataa agatggtacg ttaattgaat ttgatagaag ttgggaaaaa ataggggtac
     1621 gatttgtaca atcattgctt gagactttcc cagtacataa taaagaagct gctttaagac
     1681 aactcggtgt cattaaagaa tctattgatc caaaatcagt gatgggttca ggatctttac
     1741 aacaaattat ccaggcattt aatgatgtga cgggacaaga tacaaccgac tggtccaagt
     1801 caacaagtca aaagctggta gatgaacgta ttcctgaaat taattgggta gaaggtgtta
     1861 aagaagcact tatcgatttg aaagcaaaag gctatcaact tggtattgtt acgagtgata
     1921 ctaaaaaagg tgtagaacaa tttttagcac ataccaatgc tacctcgttg ttcgatttga
     1981 tcatttctac cgaagcggat gcctatgaga agccaaatcc taaagtatta tcgcctttat
     2041 ttgagcaaaa taatgtagat cctcagaaag tagctatagt aggagacact gctaatgata
     2101 tgaagacagc aagtaatgca aatttaggta tggcaatagg tgtattaaca ggtattgcaa
     2161 caaaagaaga attacatgaa gctgatatta ttttaaatag tgcggcagat attttagaag
     2221 ctttaaatta aaaagaaaga catagtgtat atgtgaatta atgtattcga tagaattgat
     2281 aaaataatcg ataatatcta tggaatatac attgaatata aacatatacg ctatgtcttt
     2341 agtcttttat cgtgtatcta cttgtcgata tgtttgaata attcgagcaa ttttgtttat
     2401 cataggattt aaagattcgg ggtccttatg gatatcatat tcattaatgt tgatacgtac
     2461 cacaggacat gcattaaagc tattaatcca atcgtcatag cgtttaaata gctttttcca
     2521 gtattcaggg tctgtattaa tttccatttc gcgaccacgt tcaataatac gatcaatgac
     2581 ctcatcatag ttacattcta aataaatcat tacatcaggt ttaggaaaat aaggtgtcat
     2641 gaccatggca ttaaataagt ctgaatatgt tttgaaatct tctttactca ttgtgccttc
     2701 ttcttcatgc atttttgcaa aaatatcaac atcttcataa attgatcgat cttggacaaa
     2761 gccaccacca tattcaaaca tacgcttttg ttctttaaaa cgttcagcta agaagtaaat
     2821 ttgcaaatgg aaactccatc gttcaaaatc gctgtaaaat ttatctaaat atggattatg
     2881 ttcgacattt tcaaaagacg ttttaaagtt taatttatct gcaagtgctt gcgttagtgt
     2941 tgattttcca acaccaactg tacctgcaat ggttataatg gcattttgtg gaataccgta
     3001 attattcatt ggtaatatct cctatcatag gtaatataat atgtaatata tcttcgtaat
     3061 cttgttcatt tttaagaaaa tcaatagaag ttgtatcgat taaaactaca tttgaaccat
     3121 tactttgtaa ggactcataa tactcacgat aatctttttt taactttaac agatattcat
     3181 cttctatttg atgctcaaaa ctacggttac gtttagcaat tctagatttt aacacatcaa
     3241 ggtctgcatc taaaaagata atcatattcg gcataatcat atcttcagtt aaaatatcat
     3301 aaattttact gaatttctga aattcaacag aactcaaagt atttttagca aatatcttat
     3361 ttttatgtat atgataatca ctaactacac cttgatttag ttgtgttaca tcttgaaatt
     3421 gcttatatct attgcataaa aagaacattt cagtttgaaa actccattta gagatatctt
     3481 cataaaagtc tgataaaaat ggattttctg tgatgatttc tttttcttca taaaaatcta
     3541 aagtttgact taatttgtgt gcaagtgaag atttacctac gccaatagga ccttcaattg
     3601 ctataaaagg tttgttcata ttcacatctc caaatgttaa aatagacatt gagtattgta
     3661 ccataagtga ggtatctgga cataaatgac aaatgatata tattttatga cattagcgat
     3721 tgaagaagct aaaaaagcag ctcaactagg cgaagtacct ataggtgcta tcatcactaa
     3781 agatgatgaa gttatcgcta gagcacataa tttaagagaa acactacaac aaccaacggc
     3841 gcatgctgaa catattgcaa ttgaacgtgc agccaaagtg ttaggtagtt ggcgtttaga
     3901 aggttgcaca ttatatgtaa ccttagaacc atgtgtcatg tgcgcaggaa caattgtaat
     3961 gagtcgcatt ccaagagtcg tctatggcgc agatgatcct aaaggtggtt gtagtggcag
     4021 tttaatgaat ttattgcaac aatctaattt taatcatcgt gcaattgttg ataaaggtgt
     4081 acttaaagaa gcatgtagca cattattaac aacatttttt aaaaacttaa gagccaataa
     4141 gaaatccacc aattagacga tagtacatac taattaaaat ttgttagaat tacattatat
     4201 gataaattaa tgacaatata aatgttaata gaaaagtatg tgcgctgagt atatattctt
     4261 atttaagaac aagattttaa atttacgaaa cgaggtaaca taatgataaa actaatagcc
     4321 actgatatgg atggcacgct acttaatgca gcacatgaaa tttctcaacc taatattgat
     4381 gcgattaaat acgctcaaga acaagggata acggttgtta tcgcgacagg tcgagcattt
     4441 tatgaagcac aagcaccagt tgctgacaca gatttaacag taccatatat ttgtttgaat
     4501 ggtgctgaag tacgtgatga aactttcaat gtaatgagca cttcacacct taataaatcg
     4561 ttagtacaca aaattacaaa tgttttaaaa gatgcaggta tttattatca agtatacacg
     4621 agtcgtgcga tttatactga agatccacaa agagatttag acatttacat agatattgct
     4681 gagcgtgcag gtcaacatgc aaacgttgag cgtattaaaa atggtattca aagacgcata
     4741 gataatggta cgttgaaagt tgttgataat tatgatgcta ttgaaaacat acctggtgaa
     4801 ttaattatga aaatattagc atttgatgga aatttagaaa aaattgacaa agctagtaaa
     4861 attttagctg aatctccgaa tttagctata tcatcatctt cgagaggaaa tatagaaata
     4921 acgcattcag atgcacaaaa aggtattgcg ctagaaacaa ttgccgaaag attagggatt
     4981 gaaatgaaag aagtcatggc aataggtgac aatttaaatg acttatcaat gttagagaaa
     5041 gttggctatc cagttgcgat ggaaaatggt gcagaagaag ttaaaaaaat agcgaaatat
     5101 gtcacagata cgaatgaaaa tagtggtgtt ggaaaagcta ttatgaaatt attacgtgaa
     5161 caacaagttt aataaaaaaa gaggggtcaa atatgaaagg attaattatt attggcagtg
     5221 cacaagtgaa ttcacataca agtgcactag caagatactt aactgagcat tttaaaacac
     5281 atgatattga agcggaaata ttcgatttag cagaaaagcc gttaaatcaa ttagattttt
     5341 caggaacaac accgtctatt gatgaaatca aacaaaatat gaaagattta aaagagaaag
     5401 caatggcggc ggacttttta atattaggaa cgccaaacta tcatggttca tattctggaa
     5461 tattgaaaaa tgcattagat catctaaata tggattattt taaaatgaaa cctgtaggct
     5521 taataggaaa tagtggtggt attgttagtt cagagccatt gtcacattta agagtaatcg
     5581 tcagaagttt actaggcatt gctgtaccaa ctcaaatagc aacacatgat tctgattttg
     5641 ctaaaaatga agatggttca tattacttaa atgatagtga attccaatta cgagcaagat
     5701 tatttgtcga tcaaattgta tcttttgtga ataatagtcc atatgaacat ttaaaataat
     5761 attaaaaaat atgtaaatat acattaaaaa taggtatgta gcttaaggta tttcattgag
     5821 aatgctttta agtcacatgc ctattttttg ttctaatgca ccaatagtaa gcctttgaga
     5881 ttactgttgt gagcaatgtt attaattaaa ataagatgta atagcgaatt gaaaacaagc
     5941 ttagattaaa agtgagtttt aaaaatatag ataaataatt taaatcaatt aaagaaaaaa
     6001 agtattaaaa attgcaattc ttaaactgtg atttatatta atatttgtag caaataacat
     6061 tgtaaaacaa agaaaaataa ttaaagtatt gtactttatc taaaaatgta atactatatt
     6121 gatatacatt tttgtattta aaacaattgt attatttaaa aatttgatga attaggagta
     6181 atctaatgct aaacagagaa aataaaacgg caataagaag aaaaggcatg gtatccaatc
     6241 gattaaataa attttcgatt agaaagtaca cagtgggaac agcatcaatt ttagtaggta
     6301 caacattaat ttttggtctg gggaaccaag aagcaaaggc tgcagaaagt actaataaag
     6361 aattgaacga agcgacaact tctgcaagtg ataatcaatc gagtgataaa gttgatatgc
     6421 agcaactaaa tcaagaagac aatactaaaa atgataatca aaaagaaatg gtatcatctc
     6481 aaggtaatga aacgacttca aatgggaata aattaataga aaaagaaagt gtacaatcta
     6541 ccactggaaa taaagttgaa gtttcaactg ccaaatcaga tgagcaagct tcaccaaaat
     6601 ctacgaatga agatttaaac actaaacaaa ctataagtaa tcaagaagcg ttacaacctg
     6661 atttgcaaga gaataaatca gtggtaaatg ttcaaccaac taatgaggaa aacaaaaagg
     6721 tagatgcgaa aactgaatca actacattaa atgttaaaag tgatgctatc aagagtaatg
     6781 ctgaaactct tgttgataac aatagtaatt caaataatga aaataatgca gatatcattt
     6841 tgccaaaaag tacagcacct aaacgtttga atacaagaat gcgtatagca gcagtacagc
     6901 catcatcaac agaggctaaa aatgttaatg atttaatcac atcaaataca acattaactg
     6961 tcgttgatgc agataatagc aaaacgattg taccagccca agattattta tcattaaaat
     7021 cacaaattac agttgatgac aaagttaaat caggtgatta tttcacaatt aaatactcag
     7081 atacagtaca agtatatgga ttgaatccgg aagatattaa aaatattggt gatattaaag
     7141 atccaaataa tggtgaaaca attgcgactg caaaacatga tactgcaaat aatttaatta
     7201 catatacatt tacagattat gttgatcgat ttaattcagt aaaaatgggt attaattact
     7261 caatttatat ggatgcagat acaattcctg ttagtaaaaa cgatgttgag tttaatgtta
     7321 cgataggtaa tactacaaca aaaacaactg ctaacattca atatccagat tatgtttcta
     7381 gagataataa ttctataggt tcagcattta ctgaaactgt ttcacatgca ggtaatgcag
     7441 aagacccggg ttactatata cagactgttt atgtaaatcc atcggaaaaa actttaacaa
     7501 atgctaaatt aaaagttgaa gcttatcata aggattatcc tgacaatgta ggtcaaataa
     7561 ataaaaatgt aactaaaatt aaaatttatc aagcaccaaa agactatgtt ttaaacaaag
     7621 gttatgatgt taatacaaat caacttatag atgtgactga acaatttaaa gataagatta
     7681 catacggaac taatgacagt gtaaatgttg attttggtag cattaacaat tcttatgtag
     7741 ttatggtgga tacaaaattt gaatttacaa ctagtgaaag tccaacactt gttcaaatgg
     7801 caactttgac ttctgatgga aacagatccg tttctactgg caatgcttta ggatttacta
     7861 ataaccaaag tggtggtgct ggtcaagaag tctataaaat tggtaactac gtttgggaag
     7921 atacaaataa aaatggtatt caagaattag gggaagttgg agtaaaaggt gtaacagtgg
     7981 ttgcatatga caataaaact aataaagaag ttggaagaac gattactgat gaaaaaggag
     8041 ggtacttgat tccaaactta cctaatggag attaccgtgt agaattttca aacttaccaa
     8101 aaggttatga agtaacccct tcaaaacaag gtaataacga agaattagat tcaaacggct
     8161 tatcttcagt tattacagtt aatggcaaag ataacttatc tgcagactta ggtatttaca
     8221 aacctaaata caacttaggt gactatgtct gggaagatac aaataaaaat ggtatccaag
     8281 accaagatga aaaaggtata tctggcgtaa cggtaacatt aaaagatgaa aacggtaacg
     8341 tgttaaaaac agtaacaaca gacgctgatg gcaaatataa atttactgat ttagataatg
     8401 gtaattataa agttgaattt actacaccag aaggctatac accgactaca gtaacatctg
     8461 gtagcgacat tgaaaaagac tctaatggtt taacaacaac aggtgttatt aatggtgctg
     8521 ataacatgac attagatagt ggattctaca aaacaccaaa atataattta ggtaattatg
     8581 tatgggaaga tacaaataaa gatggtaagc aggattcaac tgaaaaaggt atttcaggcg
     8641 taacagttac attgaaaaat gaaaacggtg aagttttaca aacaactaaa acagataaag
     8701 atggtaaata tcaatttact ggattagaaa atggaactta taaagttgaa ttcgaaacac
     8761 catcaggtta cacaccaaca caagtaggtt caggaactga tgaaggtata gattcaaatg
     8821 gtacatcaac aacaggtgtc attaaagata aagataacga tactattgac tctggtttct
     8881 acaaaccgac ttacaactta ggtgactatg tctgggaaga tacaaataaa aacggtgttc
     8941 aagataaaga tgaaaagggc atttcaggtg taacagttac gttaaaagat gaaaacgaca
     9001 aagttttaaa aacagttaca acagatgaaa atggtaaata tcaattcact gatttaaaca
     9061 atggaactta taaagttgaa ttcgagacac catcaggtta tacaccaact tcagtaactt
     9121 ctggaaatga tactgaaaaa gattctaatg gtttaacaac aacaggtgtc attaaagatg
     9181 cagataacat gacattagac agtggtttct ataaaacacc aaaatatagt ttaggtgatt
     9241 atgtttggta cgacagtaat aaagacggca aacaagattc aactgaaaaa ggtatcaaag
     9301 atgttaaagt tactttatta aa
//
LOCUS       DAHHYJ010000104         9267 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000104 DAHHYJ010000000
VERSION     DAHHYJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.104"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(282..833)
                     /locus_tag="NBK22_001936"
     CDS             complement(282..833)
                     /locus_tag="NBK22_001936"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD2415119.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            1198..1824
                     /locus_tag="NBK22_001937"
     CDS             1198..1824
                     /locus_tag="NBK22_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCD2415120.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTAVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEQNYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            1995..3107
                     /gene="pdhA"
                     /locus_tag="NBK22_001938"
     CDS             1995..3107
                     /gene="pdhA"
                     /locus_tag="NBK22_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCD2415121.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            3111..4088
                     /locus_tag="NBK22_001939"
     CDS             3111..4088
                     /locus_tag="NBK22_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD2415122.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            4179..5471
                     /locus_tag="NBK22_001940"
     CDS             4179..5471
                     /locus_tag="NBK22_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD2415123.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESVASATSEEVAETPAAPAAVTLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            5475..6881
                     /gene="lpdA"
                     /locus_tag="NBK22_001941"
     CDS             5475..6881
                     /gene="lpdA"
                     /locus_tag="NBK22_001941"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD2415124.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            7049..7324
                     /locus_tag="NBK22_001942"
     CDS             7049..7324
                     /locus_tag="NBK22_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCD2415125.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            7468..8007
                     /locus_tag="NBK22_001943"
     CDS             7468..8007
                     /locus_tag="NBK22_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2415126.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            8020..9114
                     /locus_tag="NBK22_001944"
     CDS             8020..9114
                     /locus_tag="NBK22_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2415127.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            9107..>9267
                     /locus_tag="NBK22_001945"
     CDS             9107..>9267
                     /locus_tag="NBK22_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415128.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYL"
BASE COUNT         3369 a         1333 c         1789 g         2776 t
ORIGIN      
        1 acatgatgaa tgataataaa taaagcagtt tatctcacta gaaatctaag tatttcgaat
       61 aaatgtgcag ttctatttat taaaataaat gtagagatta taaattaatg ttaagaaaga
      121 attaaaggat tttatatgaa acggtaaaat ttaaatgagg ggataaaaag tttggtagaa
      181 aggtgaatat atttaactgt tgtttattcg aaaaagttgt acaagaaaaa gcttaggaca
      241 tcatatttat cctaagcttt gataattagt aatagtgtgt tttaaacttc tactgcatct
      301 gtatgtggtt gtaatgggtg gtctttgtca atgtgatcat agaacattac accatttaaa
      361 tgatcaattt catgttggaa aacaattgct ggatatcctt ttagtcgtaa ttgtatatca
      421 ttaccttcga tgtctttggc tttaattgta attctattat gacggtgaac tagaccagca
      481 acattatcat cgacactaag gcaaccttca ccagttggta aataagcttc ttgaacgcta
      541 tgacttacaa tttttgggtt cacaagcata tagtcataag atttgccact gccatcatct
      601 ggtattaaaa cagcaatcat acgtttagaa atattaattt gaggtgcagc caaaccaacg
      661 cctgaacgta aaccatatcg tttcgcgatt tcctcatctt gactatttac taaaaactct
      721 ctcatggcga ttaatgtttc tttttcttct ttagttaatg gtaattctaa ctcagctgct
      781 ttttgacgca aagttggatg accatctcta atgatgtctt tcattgttaa catatattgc
      841 accttcctta ttttaatttg ttttagttga atgacagtaa aaaggttgtt aagatactca
      901 tacattttta tgtgtaaata tctacaaagt taaccaacta ctgccaatgt ttattttaga
      961 tagtatatgt aaattttcag atatgctaat tgcttaaaaa atgattaaag tgttggtttc
     1021 aagcaatgat actttagaaa tttatttatc atcttgactt taaaaattat attataaatg
     1081 acgtaactgt caacagatat acttagtaat gaagatgtgt aatgtaattg tttaaaattg
     1141 atttccaagc agattttatt tatcatttaa tttaaatagc aagtggaggt acaagtaatg
     1201 aaatttggaa aaacaatcgc agtagtatta gcatctagtg tcttgcttgc aggatgtact
     1261 acggataaaa aagaaattaa ggcatattta aagcaagtgg ataaaattaa agatgatgaa
     1321 gaaccaatta aaactgttgg taagaaaatt gctgaattag atgagaaaaa gaaaaaatta
     1381 actgaagatg tcaatagtaa agatacagca gttcgcggta aagcagtaaa ggatttaatt
     1441 aaaaatgccg atgatcgtct aaaggaattt gaaaaagaag aagacgcaat taagaagtct
     1501 gaacaagact ttaagaaagc aaaaagtcac gttgataaca ttgataatga tgttaaacgt
     1561 aaagaagtaa aacaattaga tgatgtatta aaagaaaaat ataagttaca cagtgattac
     1621 gcgaaagcat ataaaaaggc tgtaaactca gagaaaacat tatttaaata tttaaatcaa
     1681 aatgacgcga cacaacaagg tgttaacgaa aaatcaaaag caatagaaca gaactataaa
     1741 aagttaaaag aagtatcaga taagtataca aaagtactaa ataaggttgg taaagaaaag
     1801 caagacgttg atcaatttaa ataattaata taatacagat ggtaggaaac aactaataca
     1861 gttcctatta tctgtatctt tttttattaa aacagaactt tttcaaatgg tttaacagtc
     1921 ccatttattt gtggtacaat tagtaaggat aaaatgaatt tctatacaat tatgggaaag
     1981 gtatggtgaa ttgaatggct cctaagttac aagcccaatt cgatgcagta aaagttttaa
     2041 atgatactca atcgaaattt gaaatggttc aaattttgga tgagaatggt aacgtcgtaa
     2101 atgaagactt agtacctgat cttacggatg aacaattagt ggaattaatg gaaagaatgg
     2161 tatggactcg tatccttgat caacgttcta tctcattaaa cagacaagga cgtttaggtt
     2221 tctatgcacc aactgctggt caagaagcat cacaattagc gtcacaatac gctttagaaa
     2281 aagaagatta cattttaccg ggatacagag atgttcctca aattatttgg catggtttac
     2341 cattaactga agctttctta ttctcaagag gtcacttcaa aggaaatcaa ttccctgaag
     2401 gcgttaatgc attaagccca caaattatta tcggtgcaca atacattcaa gctgctggtg
     2461 ttgcatttgc acttaaaaaa cgtggtaaaa atgcagttgc aatcacttac actggtgacg
     2521 gtggttcttc acaaggtgat ttctacgaag gtattaactt tgcagcagct tataaagcac
     2581 ctgcaatttt cgttattcaa aacaataact atgcaatttc aacaccaaga agcaagcaaa
     2641 ctgctgctga aacattagct caaaaagcaa ttgctgtagg tattcctggt atccaagttg
     2701 atggtatgga tgcgttagct gtatatcaag caactaaaga agcacgtgac cgcgcagttg
     2761 caggtgaagg tccaacatta attgaaacta tgacatatcg ttatggtcct catacaatgg
     2821 ctggtgacga tccaactcgt tacagaactt cagacgaaga tgctgaatgg gagaaaaaag
     2881 acccattagt acgtttccgt aaattccttg aaaacaaagg tttatggaat gaagacaaag
     2941 aaaatgaagt tattgaacgt gcaaaagctg atattaaagc agcaattaaa gaggctgata
     3001 acactgaaaa acaaactgtt acttctctaa tggaaattat gtatgaagat atgcctcaaa
     3061 acttagcaga acaatatgaa atttacaaag agaaggagtc gaagtaagcc atggcacaaa
     3121 tgacaatggt tcaagcgatt aatgatgcgc ttaaaactga acttaaaaat gaccaagatg
     3181 ttttaatttt tggtgaagac gttggtgtta acggcggtgt tttccgtgtt actgaaggac
     3241 tacaaaaaga atttggtgaa gatagagtat tcgatacacc tttagctgaa tcaggtattg
     3301 gtggtttagc gatgggtctt gcagttgaag gattccgtcc ggttatggaa gtacaattct
     3361 taggtttcgt attcgaagta tttgatgcga ttgctggaca aattgcacgt actcgtttcc
     3421 gttcaggcgg tactaaaact gcacctgtaa caattcgtag cccatttggt ggtggcgtac
     3481 acacacctga attgcacgca gataacttag aaggtatttt agctcaatct ccaggtctaa
     3541 aggttgttat tccttcaggc ccatacgatg cgaaaggttt attaatttct tctattagaa
     3601 gtaatgaccc agtcgtatac ttggagcata tgaaattgta tcgttcattc cgtgaagaag
     3661 tgcctgaaga agaatataca attgacattg gtaaggctaa tgtgaaaaaa gaaggtaatg
     3721 acatttcaat catcacatac ggtgcaatgg ttcaagaatc aatgaaagct gcagaagaac
     3781 ttgaaaaaga tggttattct gttgaagtaa ttgacttacg tactgtccaa ccaatcgatg
     3841 ttgacacaat tgtagcttca gttgaaaaaa ctggtcgtgc agtagtagtt caagaagcac
     3901 aacgtcaagc tggtgttggt gcagcagttg tagctgaatt aagtgaacgt gcaatccttt
     3961 cattagaagc acctattgga agagttgcag cagcagatac aatttatcca ttcactcaag
     4021 ctgaaaatgt ttggttacca aacaaaaatg acatcatcga aaaagcaaaa gaaactttag
     4081 aattttaata cattttaaaa gttaacgaag ttagcgtatt ttagtctcat tgattaaaat
     4141 gaaatgttta atttacgaaa tcttaggagg gcaaaaacgt ggcatttgaa tttagattac
     4201 ccgatatcgg ggaaggtatc cacgaaggtg aaattgtaaa atggtttgtt aaagctggag
     4261 atactattga agaagacgat gttttagctg aggtacaaaa cgataaatca gtagtagaaa
     4321 tcccatcacc agtatctggt actgtagaag aagttatggt agaagaaggt acagtagctg
     4381 tagttggtga cgttattgtt aaaatcgatg cacctgatgc agaagatatg caatttaaag
     4441 gtcatgatga tgattcatca tctaaagaag aacctgcgaa agaggaagcg ccagcagagc
     4501 aagcacccgt agctactcaa actgaagaag tagatgaaaa cagaactgtt aaagcaatgc
     4561 cttcagtacg taaatacgca cgtgaaaaag gtgttaacat taaagcagtt tctggatctg
     4621 gtaaaaatgg tcgtattaca aaagaagatg tagatgcata cttaaatggt ggtgcaccaa
     4681 cagcttcaaa tgaatcagtt gcttcagcta caagtgaaga agttgctgaa actcctgcag
     4741 cacctgcagc agtaacatta gaaggcgact tcccagaaac aactgaaaaa atccctgcta
     4801 tgcgtagagc aattgcgaaa gcaatggtta actctaagca tactgcacct catgtaacat
     4861 taatggatga aattgatgtt caagcattat gggatcaccg taagaaattt aaagaaatcg
     4921 cagctgaaca aggtactaag ttaacattct taccttatgt tgttaaagca cttgtttctg
     4981 cattgaaaaa atacccagca cttaacactt cattcaatga agaagctggt gaaatcgttc
     5041 ataaacatta ctggaatatc ggtattgcag cagacactga tagaggatta ttagtacctg
     5101 ttgttaaaca tgctgatcgt aagtctattt tccaaatttc agatgaaatt aatgaattag
     5161 ctgttaaagc acgtgatggt aaattaacag ccgatgaaat gaaaggtgct acatgcacaa
     5221 tcagtaatat cggttcagct ggtggacaat ggttcactcc agttatcaat cacccagaag
     5281 tagcaatctt aggaattggc cgtattgctc aaaaacctat cgttaaagat ggagaaattg
     5341 ttgcagcacc agtattagca ttatcattaa gctttgacca cagacaaatt gatggtgcaa
     5401 ctggccaaaa tgcaatgaat cacattaaac gtttattaaa taatccagaa ttattattaa
     5461 tggaggggta aaacatggta gttggagatt tcccaattga aacagatact atagtaatcg
     5521 gagcaggtcc tggtggatac gttgcagcaa ttcgtgcagc tcaattagga caaaaagtaa
     5581 caatcgttga gaaaggtaat cttggtggtg tttgcttaaa cgtaggatgt attccttcaa
     5641 aagcattact acatgcttct caccgttttg ttgaagcaca acattctgaa aacttaggtg
     5701 ttattgctga aagtgtttct ttaaacttcc aaaaagttca agaattcaaa tcatcagttg
     5761 ttaataaatt aactggtggt gttgaaggct tacttaaagg taacaaagtt aacatcgtta
     5821 aaggtgaagc atatttcgta gataacaata gcttacgtgt tatggacgaa aagagcgcac
     5881 aaacatacaa ctttaaaaat gcaatcattg caacaggttc aagaccaatt gaaattccta
     5941 atttcaaatt cggtaaacgt gttatcgact caacaggtgc tttaaactta caagaagtac
     6001 caggtaaatt agttgtagtt ggtggaggat acattggatc agaattaggt acagcatttg
     6061 ctaactttgg ttcagaagta accatccttg aaggtgctaa agatatctta ggtggcttcg
     6121 aaaaacaaat gacacaacct gttaaaaaag gtatgaaaga aaaaggtgtt gaaatcgtta
     6181 ctgaagctat ggctaaatca gctgaagaaa cagataacgg agttaaagtt acttatgaag
     6241 ctaaaggcga agagaaaaca atcgaagctg attatgtatt agtaactgta ggtcgtcgtc
     6301 caaacacaga cgaattaggc ctagaagaat taggtgttaa attcgctgac cgtggattat
     6361 tagaagttga taaacaaagc cgtacgtcta tcagcaatat ctatgcaatt ggtgatatcg
     6421 ttccaggttt accacttgct cacaaagcta gctatgaagc taaagttgct gctgaagcaa
     6481 ttgatggtca agctgctgaa gttgattaca ttggtatgcc agcagtatgc tttactgaac
     6541 cagaattagc tacagttggt tattcagaag cgcaagctaa agaagaaggt ttagcaatta
     6601 aagcttctaa attcccatat gcagcaaatg gtcgtgcatt atcattagat gatactaacg
     6661 gatttgttaa acttattaca cttaaagaag atgatacttt aatcggtgct caagtagttg
     6721 gtactggtgc atcagatatt atctctgaat taggtttagc aattgaagct ggtatgaatg
     6781 ctgaagatat cgcattaaca atccatgcac atccaacatt aggtgagatg actatggaag
     6841 cagcagaaaa agctatcgga tacccaatcc atacaatgta ataactgatt atctataaag
     6901 attcagtcat taaaagctgt agcatatgct acggcttttt tgttttaggt aaagtaatgt
     6961 aaggaaattg atttgagata tcgttaacat gtgacatgca tgttatacta gcgatgctaa
     7021 caaaagaatt gaaatggagg gttcaacaat ggaatatgag tatccaattg atttagactg
     7081 gagtaatgaa gagatgattt cagtgataaa tttctttaat catgtagaga agtattatga
     7141 atccggcgtg acggcaggcg actttatggg tgcatataaa agatttaaag aaattgtgcc
     7201 tgctaaagca gaggaaaaac aaatttttaa tactttcgaa aaaagtagtg gctataatag
     7261 ttacaaagca gttcaagatg taaaaactca ctctgaagaa caaagagtaa cagctaaaaa
     7321 ataattcgtt cgaaattaac acaatttaat aggaattttt ctttaaaact attgctaata
     7381 aagctatatt ttgatacctt tatcaagtgt taaacaaaat gtttgataaa agtaaactta
     7441 atatagcttt tttaggtgga aaaataaatg aacataggta ataaaattaa aaatcttaga
     7501 agaattaaaa atttaacgca agaagaactt gctgaacgta cagacttatc gaaaggctac
     7561 atttcacaaa tagaaagtga acatgcctca ccaagtatgg aaactttctt aaatattata
     7621 gaggtgttag gaacgacgcc aagtgaattt tttaaagaca gtgaaaatga aaaagtatta
     7681 tacaagaagg aagaacaagt tatttatgat gagtatgatg aaggttatat attaaattgg
     7741 ttagtttcaa agtcaaatga atatgatatg gagccattaa tattaacttt aaagcctgga
     7801 gcatcatata aaaattttaa tccatcagag tctgatacgt ttatttattg tatgtcaggt
     7861 cagataacac ttaatttagg caaagagata tatcaagcac aagaagaaga cgttttgtat
     7921 tttaaagcac gagataatca tcgtttgtca aatgaatcaa acaatgaaac acgaatactt
     7981 attgtagcga cagcttcata tttatagggg ggatcttatt tggaaccgtt attatcatta
     8041 aaatcagtta gtaaaagcta tgatgatctt aatatcttag atgacataga tattgatatt
     8101 gaatcaggat acttttatac attattaggt ccttcaggtt gtggtaaaac aacaatttta
     8161 aaattaattg cagggtttga atatcctgac agtggtgaag tgatttatca aaacaaacca
     8221 attggtaatt taccaccaaa taaacgtaaa gtgaatacag tctttcaaga ttatgcatta
     8281 tttccacact taaacgtcta tgataatatc gcttttggtt tgaaattaaa aaaattatca
     8341 aaaaccgaaa ttgatcaaaa agtaactgag gcattaaaat tagtaaaact ttcaggttat
     8401 gaaaaaagaa atattaatga aatgagtggc ggacaaaagc aacgtgttgc aattgcacgt
     8461 gctatcgtaa atgaaccaga aatattattg ttagatgaat ctttatccgc attagatttg
     8521 aaattgcgta ctgaaatgca atatgaatta cgagaattgc aatctagatt aggtattaca
     8581 tttatatttg taacacatga tcaagaagaa gcgttagcat taagtgactt tctttttgta
     8641 ttaaaagatg ggaaaattca acaatttggc acaccaacag atatatatga cgaaccagtg
     8701 aatcgatttg tagctgattt tattggagaa tctaatattg ttgaagggcg catggttaga
     8761 gattatgtcg tgaatattta tgggcaagat ttcgaatgtg tcgatatggg tattcctgaa
     8821 aataaaaaag tagaagtcgt tattcgacca gaagatatat cattaatcaa agctgaagaa
     8881 ggattattta aagcaactgt tgattctatg ttatttagag gggtccacta tgaaatatgt
     8941 tgtatagaca ataaaggtta tgaatgggta atacaaacga ctaaaaaagc tgaagtaggc
     9001 agtgaagttg gtctttattt tgatcctgaa gccattcata ttatggttcc tggagaaaca
     9061 gaagaagaat ttgataaacg tattgaaagc tatgaggaag tagacaatgc gtaatactaa
     9121 taaatttctc ttaatcccgt atttactatg gatggttata tttattattg taccagttgt
     9181 attactcatt tatttttcat ttttagatat caatggacat tttagtttca cgaattatca
     9241 acaaattttt actacaaaat atttgaa
//
LOCUS       DAHHYJ010000105         9263 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000105 DAHHYJ010000000
VERSION     DAHHYJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.105"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            292..1389
                     /locus_tag="NBK22_001946"
     CDS             292..1389
                     /locus_tag="NBK22_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCD2415129.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQHALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVVIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVRNYNNVKHKDVLIVGNSFNNSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINVDEIKK"
     gene            1594..3156
                     /locus_tag="NBK22_001947"
     CDS             1594..3156
                     /locus_tag="NBK22_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCD2415130.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGKMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(3167..3274)
                     /locus_tag="NBK22_001948"
     CDS             complement(3167..3274)
                     /locus_tag="NBK22_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415131.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(3346..4140)
                     /locus_tag="NBK22_001949"
     CDS             complement(3346..4140)
                     /locus_tag="NBK22_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCD2415132.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGTDSISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            complement(4160..5236)
                     /locus_tag="NBK22_001950"
     CDS             complement(4160..5236)
                     /locus_tag="NBK22_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCD2415133.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            5420..6889
                     /locus_tag="NBK22_001951"
     CDS             5420..6889
                     /locus_tag="NBK22_001951"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCD2415134.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLAQGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            6882..7703
                     /gene="nadE"
                     /locus_tag="NBK22_001952"
     CDS             6882..7703
                     /gene="nadE"
                     /locus_tag="NBK22_001952"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCD2415135.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEP
                     DEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELGYTRYTWPKS"
     gene            7974..8576
                     /locus_tag="NBK22_001953"
     CDS             7974..8576
                     /locus_tag="NBK22_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCD2415136.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            8557..8730
                     /locus_tag="NBK22_001954"
     CDS             8557..8730
                     /locus_tag="NBK22_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCD2415137.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQKDGTVEV
                     SHQEIVFVGKKIQ"
     gene            complement(9022..>9263)
                     /locus_tag="NBK22_001955"
     CDS             complement(9022..>9263)
                     /locus_tag="NBK22_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCD2415138.1"
                     /translation="HPQDLNDSPLKFILSTSDDSDYICKYINTEHKQLTLYNKNNSSI
                     VIEIFIPNDNKILLTIMNTEALGTSPRMTFIKHKS"
BASE COUNT         3217 a         1291 c         1705 g         3050 t
ORIGIN      
        1 atatataaat atagattaag tatatagatt aatcaacttt tttggaagag caaatcacgc
       61 aatcaacaaa taatataaga agtttttgcg atagttttaa aatagctgta atagaatact
      121 aaatgtgaca aacttagaac taatatcaag tgttgatgtt ttgaatataa aaatgctaat
      181 agatggcatc acgagattta tgactatctg ttaaatcaac ctaacacgat tataatttga
      241 ataattggtt aatatatgag taattagaaa atagacaaag gatgacgatt tatgtatatc
      301 aatatgaaag attatgggtt aacaggcata aacaaaacta aagatactcg agcaatacaa
      361 catgcgttaa atcgtggaag atgtaaacca acgacagttt atataccgaa agggacgtat
      421 gatatttgca aaccattaac gatatatggc aatacaacac ttttgttgga taatgaaact
      481 attttacgcc gatgtcattc tggtccttta ttaaaaaatg gtcatcgctt tggtttttat
      541 cgtggttata atggacacag tcatattcat attaaaggcg gcaagtttga tatgaatggc
      601 gtatcgtatc cttataacaa tacagctatg tgcattgggc atgctgaaga tattcaatta
      661 ataggtgtga ctattaaaaa tgtagtgagt ggtcatgcaa ttgatgcttg cgggattaat
      721 ggactctata ttaaaagctg ttcatttgaa ggatttatag actatagtgg cgaacgcttt
      781 tattctgaag caatacaatt agacattcaa gtacctggtg cttttccaaa attcggaacg
      841 acagatggta cgataacgaa aaatgtcgtt atcgaagatt gttattttgg accttcagaa
      901 ttgcccgaaa tgggaagttg gaatcgtgct attggctcac atgcaagtag acataatcgt
      961 tactatgaga atattcatat tagaaataat atatttgaag atatacaagg ttatgcatta
     1021 actcccttga agtataaaga tgctttcatt attaataata agtttattaa ctgtgagggt
     1081 ggtattagat atttaggagt tagagatggt aaaaatgcag cagatgtgat gacaggaaaa
     1141 gacttaggtt cccaagcagg cataaatatg aatataattg gaaatgaatt taaaggatca
     1201 atgtctaaag atgcgataca tgtacgtaat tataataatg ttaaacataa agatgtatta
     1261 atcgttggga attcattcaa taattcgact caatcaattc atttagaaga tattgataca
     1321 gtgtttttaa gtcctgttga agcgggtatt caagttacta caatcaatgt agatgaaata
     1381 aaaaagtaaa aagtttcgca tgacattagg attaagaata gcagataatt tttgaaagcg
     1441 cattcataaa acggtataaa tatgctataa taaacccaat tatctgataa aaggggtatt
     1501 ttgacggtaa tgataataca agatagacaa ctttctatac tctaatatag tgagttgaag
     1561 tagcttgtca taatcatcat gagggggaaa tttatggctt atttcaatca acatcaatca
     1621 atgatatcga aaaggtattt aacattcttt tcaaaatcaa agaaaaagaa accgtttagt
     1681 gcgggacaac ttattggact aatattaggt ccattacttt tcctattaac attattattc
     1741 tttcatccac aagacttacc ttggaaaggc gtctatgttt tagcgattac tttatggatt
     1801 gcgacttggt ggattactga agcaattcct attgcagcaa cgagcttatt accaattgtg
     1861 ttattaccat taggtcatat acttacacca gaacaagtat catccgaata tggcaatgat
     1921 attatctttt tgtttttagg tggatttatt ttggcaattg caatggaaag atggaattta
     1981 catacgaggg tagcgttgac aattattaat ttgattggtg caagcacttc aaaaatatta
     2041 cttggattca tggtggcaac aggattctta tctatgtttg tatcgaacac tgcagctgta
     2101 atgattatga ttccgattgg tttagcaatt attaaggaag cacatgattt acaagaagcc
     2161 aatacgaatc aaacaagtat tcaaaagttt gaaaaatctc tagttttagc aattggctat
     2221 gcaggtacga ttggtggctt gggtacatta atcggaaccc cgccattaat tattttaaaa
     2281 ggacaataca tgcaacattt tggacatgaa attagttttg ctaaatggat gattgtaggg
     2341 attccaacgg tcattgtttt gttaggtatt acttggctct atttaagata tgttgcgttt
     2401 agacatgatt tgaaatattt acctggtggt cagacgttaa ttaaacaaaa gttagacgag
     2461 cttggcaaaa tgaagtatga agaaaaggta gtacaaacta tctttgtact tgctagctta
     2521 ttatggatta caagagagtt tcttctgaaa aaatgggaag ttacgtcatc cgttgcagat
     2581 ggtacgattg ctatttttat atcaatatta ttatttatta ttccagctaa aaatactgaa
     2641 aaacatcgcc gtatcattga ctgggaagtt gcaaaagagc tcccttgggg tgtattaatt
     2701 ttatttggtg gcggtttagc attagcgaaa ggtatttctg aaagtggttt agcaaaatgg
     2761 ttaggcgaac agttgaaatc attaaatggt gttagtccga ttcttattgt aattgtcata
     2821 acaatctttg tcttattttt aactgaagtg acatctaata ctgcaactgc aacgatgatt
     2881 ttaccgattt tagcaacgtt gtctgttgct gttggagtgc atccattact acttatggca
     2941 cctgcagcta tggcggctaa ctgtgcatac atgttaccag tagggacacc accgaatgca
     3001 attatctttg gttcaggtaa aatatctatc aaacaaatgg catcagtagg attctgggta
     3061 aacttaatca gtgcaataat tattatttta gtcgtgtatt atgtaatgcc tatagtttta
     3121 ggtattgata taaatcaacc actgccattg aaatagtaat tgcagattag aacgaaaaat
     3181 aaaaggttac attagcaatt gcttggacga gtggtaatga aacgtatacc gcagcatcgt
     3241 gtaaaaacaa tacaaacaaa agaaagtcaa ccaaggatgg attcctattt taatccttgg
     3301 ttgactcttt attttattta aattgtagaa cctagaaaat aaagtttaat taaaagcacc
     3361 aatcatttct actttgaaat ctaaggtttc taaaatagca atgactttct ttatatcggt
     3421 tgtaattgca gaatcagcct gaacgaaaaa tcgatacata cctaattgtg tttttaaagg
     3481 acgagactca atccaggata aattaatatt aaacaaagca aatgtattaa gtacacttgc
     3541 taacaaccca ggtttatcat gcattggtgt aattaaaaac atcaatgatg tcgcattttg
     3601 atcaaattgc tgctgatttt ttataactaa aaaacgtgtc acgttatgtg gatagtcttc
     3661 aatatgtgta tcaataggtg taaaaccata agcttcgcca ctacctaaag gtgcaattgc
     3721 tgcaacgcca ttttcaattt tagtcaaact ttgaattgta ctgtcgacat aatcatagtc
     3781 aaattgatgt tgatgtatgt aatttgttgt ttgactgatt gctggtgcaa tagaatacac
     3841 ttttttaata tcagaaatgg aatctgttcc attaccatat aatgcaaagt taatatctaa
     3901 acgtatttca ccgtgtgcaa agacatcttg ctgtgcaagt gcatctgcca caatgttgat
     3961 tgttccttct atagaatttt caatagggac aacaccaatc gatgtgtcat catctgcaac
     4021 tgccttgatg acttcaaata aatttgactt tggttgaaaa gttgcttcat tttcagaaaa
     4081 atactgacga caagccaaat atgaaaatgt acctttaggg cctaaataat ataattgcat
     4141 atgctacacc tctactaact taatgatgga aagggcactg gttagcattt gattctttct
     4201 ttttatagaa aaagtttgga tcttttactg tattgtcata tccgtgatga taatttgacg
     4261 tcaatgttgg agataatggc ggtgctagcc aagaccattt tccggtaact tgacgacctt
     4321 gttgtgcttc attacgttcg aatagttcga attgctttgc agcggtcaaa tgatcgacaa
     4381 ttgatacgcc ttctttttta aaggaatgat acacagcata gttcaattca acaagtgctc
     4441 gatctttatt aaatgaatta tttttaagtg tatcaaattc aaacgcatct gcaacttttt
     4501 ctagtaaatt gtaacggtaa tcatcaataa agttacgtac gccaatttca gttaccatat
     4561 accaaccgtt aaagggtgca gttggatata caatgccacc gatttttaag tccatattgg
     4621 aaatgatagg gactgcatac cattttaagt tcaattttct taattttgga taatgattat
     4681 gttcaatagg tacttcttta attaatgaag taggatattc gtaaaattta actgactcat
     4741 taggtaattg gtaaatcagt ggtaacacgt caaaattagt accttttcct ttccaaccta
     4801 agtgatttgc taagcgtgta acttcttttt cagcaggatc accacaattg tcatagccag
     4861 catagcgaat taattgattg ttgaaaattt taggtccatc ctttggagca tatatagtaa
     4921 tatacggctt taatttacct tcatttgtag cctgtgtaat atgataagta attgatgata
     4981 agaacgatgc ttcgtcagta acatctcttg catcaatgac atttaacgaa tcccaaaata
     5041 aacgaccaat gcaacgattt gaattacgcc aagccatttt agcaccataa ataagttctt
     5101 cttctgtatg tgtatatgtc ccagtttctt ttatttctag ttcaatgtca tgtaaacgtt
     5161 tattgataat ttgcgtttca taatgacact ctttatacat gttttctatg aaagcttgag
     5221 cctctttaaa taacattaac aacacctcgc tttatattat agtctacatt attaaaatac
     5281 tcttaaaaat tatgtatatg tcattaaatt gttggttgat tttaattaaa agtatggaaa
     5341 ttaaggggct cttatgtata taaaaaaatg aattatgata aaatgtaaga aaatatttag
     5401 gtcgattgga gagatacaag tgtaccaatt agaagacgac agtttaatgt tacataatga
     5461 cttatatcaa ataaatatgg ctgaaagtta ttggaatgat aatattcatg aaaaaatggc
     5521 tgtatttgat ttgtatttta gaaaaatgcc atttaatagt ggctatgctg tttttaatgg
     5581 tttaaaacgc gtcattgatt ttatagaaca ttttggattt tcagaatctg atttagaata
     5641 tttaaagtct attggctaca aggatgattt cttatcatat ttaaaagatt taaaattcac
     5701 aggcagcatc cgttcgatgc aagaaggcga gttatgcttt ggtaacgaac cattgttacg
     5761 cgtagaagca ccattgattc aagcgcaatt aatagaaaca attttattaa acattgtaaa
     5821 tttccataca ctaattacaa caaaggcaag tagaattcgt caaattgcat caaatgataa
     5881 attaatggag tttggtacac gtcgtgcgca agaaattgat gcagcattgt ggggcgctag
     5941 agctgcttac atcggaggct ttgattctac aagtaatgtt agggcgggta aattatttgg
     6001 tatacctgtg tctggtacac atgcacatgc atttgtccaa acttatggag acgaatatgt
     6061 tgccttcaaa aaatatgctg aaagacataa aaattgtgtg ttcctagtag atacgttcca
     6121 tactttaaaa tctggcgtgc caaatgcaat aaaagttgca aaagaattag gtgacaaaat
     6181 taactttgta ggtattcgat tagattctgg agatatcgct tatttatcta aagaggcaag
     6241 acgtatgctt gatgaagcag gatttactga aactaaaatt atcgcgtcta atgatttgga
     6301 tgaagaaacg attacgagtt tgaaagcaca aggtgcaaaa gtagattctt ggggcgttgg
     6361 tacaaagctg attacaggat acgatcaacc agcattaggt gcagtatata aacttgtagc
     6421 tattgaaaat gaagatggtt catatagtga tcgtattaaa ttatcaaata acgctgaaaa
     6481 ggttacgacg ccaggtaaga aaaatgtata tcgcattata aacaagaaaa caggtaaggc
     6541 agaaggcgat tatattactt tggaaaatga aaatccatac gatgaacaac ctttaaaatt
     6601 attccatcca gtgcatactt ataaaatgaa atttataaaa tctttcgaag ccattgattt
     6661 gcatcataat atttatgaaa atggtaaatt agtatatcaa atgccaacag aagatgaatc
     6721 acgtgaatat ttagcacaag gattacaatc tatttgggat gaaaataagc gtttccttaa
     6781 tccacaagaa tatccagtcg atttaagcaa ggcatgttgg gataataaac ataaacgtat
     6841 ttttgaagtt gcggaacacg ttaaggagat ggaagaagat aatgagtaaa ttacaagacg
     6901 ttattgtaca agaaatgaaa gtgaaaaagc gtatcgatag tgctgaagaa attatggaat
     6961 taaagcaatt tataaaaaat tatgtacaat cacattcatt tataaaatct ttagtgttag
     7021 gtatttcagg aggacaagat tctacattag ttggaaaatt agtacaaatg tctgttaacg
     7081 aattacgtga agaaggcatt gattgtacgt ttattgcagt taaattacct tatggagttc
     7141 aaaaagatgc tgatgaagtt gaccaagctt tgcgattcat tgaaccagat gaaatagtaa
     7201 cagtcaatat taagcctgca gttgatcaaa gtgtacaatc attaaaagaa gccggtattg
     7261 ttcttacaga tttccaaaaa ggaaatgaaa aagcgcgtga acgtatgaaa gtacaatttt
     7321 caattgcttc aaaccgacaa ggtattgtag taggaacaga tcattcagct gaaaatataa
     7381 ctggttttta tacgaagtac ggtgatggtg ctgcagatat cgcacctata tttggtttga
     7441 ataaacgaca aggtcgtcaa ttattagcgt atcttggtgc gccaaaggaa ttatatgaaa
     7501 aaacgccaac tgctgattta gaagatgata aaccacagct tccagatgaa gatgcattag
     7561 gtgtaactta tgaggcgatt gataattatt tagaaggtaa gccagttacg ccagaagaac
     7621 aaaaagtaat tgaaaatcat tatatacgaa atgcacacaa acgtgaactt ggatatacaa
     7681 gatacacgtg gccaaaatcc taatttaatt ttttcttcta acgtgtgact taaattaaat
     7741 atgagttaga attaataaca ttaaaccaca ttcagctaga ctacttcagt gtataaattg
     7801 aaagtgtatg aactaaagta agtatgttca tttgagaata aatttttatt tatgacaaat
     7861 tcgctattta tttatgagag ttttcgtact atattatatt aatatgcatt cattaaggtt
     7921 agtttgaagc agtttggtat ttaaagtgta attgaaagag agtggggcgc cttatgtcat
     7981 tcgtaacaga aaatccatgg ttaatggtac taactatatt tatcattaac gtttgttatg
     8041 taacgttttt aacgatgcga acaattttaa cgttgaaagg ttatcgttat attgctgcat
     8101 cagttagttt tttagaagta ttagtttata tcgttggttt aggtttggtt atgtctaatt
     8161 tagaccatat tcaaaatatt attgcctacg catttggttt ttcaataggt atcattgttg
     8221 gtatgaaaat agaagaaaaa ctggcattag gttatacagt tgtaaatgta acttcagcag
     8281 aatatgagtt agatttaccg aatgaacttc gaaatttagg atatggcgtt acgcactatg
     8341 ctgcgtttgg tagagatggt agtcgtatgg tgatgcaaat tttaacacca agaaaatatg
     8401 aacgtaaatt gatggatacg ataaaaaatt tagatccgaa agcatttatc attgcgtatg
     8461 aacctcgaaa catacatggt ggattctgga ctaaaggcat tcgtcgtaga aagcttaaag
     8521 attatgaacc agaagaactg gaaagtgtag tagaacatga aattcaaagt aaatgagaat
     8581 gaaacaattg ctgattgttt gtcacgaatg aaaatgcaag ggtatatgcc ggtaaaacgt
     8641 attgaaaaac ccgtgtttca agagcaaaaa gatggcacgg ttgaagtatc acatcaagaa
     8701 atcgtttttg taggtaagaa aatccaataa cataatccaa tttaaataaa gactatttga
     8761 agaggaaagg ctattcaaag tttgagtaat tttactttga atagcctatt tgtttataca
     8821 tgcaagatgc tcgatccata ttgtatgaga aacccccagc aagctatata aagcatatgc
     8881 tgggggttct taatatttta aaaattattg ttagattata tatatcgtcg ctttttctaa
     8941 aacaatctca tcgcatgaaa ttttttcttc ctagagacct ttaataagat taatagttta
     9001 cttaatcata tctagatagt cttatgactt atgcttaatg aaagtcattc taggagaagt
     9061 tcccaaagct tctgtgttca taattgttag tagtatttta ttatcatttg gtataaatat
     9121 ttcaataaca attgagctat tatttttatt atataatgtg agttgtttgt gttctgtatt
     9181 tatatatttg caaatgtaat cagaatcgtc tgaggtactg agaataaatt ttaaaggact
     9241 atcatttaaa tcttgaggat gcc
//
LOCUS       DAHHYJ010000106         9258 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000106 DAHHYJ010000000
VERSION     DAHHYJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9258
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.106"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            132..869
                     /gene="sdpB"
                     /locus_tag="NBK22_001956"
     CDS             132..869
                     /gene="sdpB"
                     /locus_tag="NBK22_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCD2415139.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YEIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYLKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(894..1586)
                     /locus_tag="NBK22_001957"
     CDS             complement(894..1586)
                     /locus_tag="NBK22_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCD2415140.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            1971..2768
                     /locus_tag="NBK22_001958"
     CDS             1971..2768
                     /locus_tag="NBK22_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCD2415141.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNEMITNEINHLWIMDPIDGTANLVKQQED
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKAIRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     ILNLKMTTLDGKAIDHLKGAPFIISNKACHETVLKILNANGGYQKYR"
     gene            2901..3848
                     /locus_tag="NBK22_001959"
     CDS             2901..3848
                     /locus_tag="NBK22_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD2415142.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVAVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
     gene            4450..7404
                     /gene="fdhF"
                     /locus_tag="NBK22_001960"
     CDS             4450..7404
                     /gene="fdhF"
                     /locus_tag="NBK22_001960"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCD2415143.1"
                     /translation="MQEHLVVTLDGKDYLVEPGMNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVLPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTAIKEKHGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIDEWVDDFDEYYK
                     SLETFTMAFAEEATGIPKSELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKAGKDNHEMVEGIHDGEVHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATYADVVLPASPSLEKDGTFTNTERRIQRLYQALEPLGDSKPDWKIFQAIANRLG
                     FDWNYKHPSEIMDEVARLTPLYAGVSYDRLEGFNSLQWPVQPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEIYDIHVNNGRLLEHFHEGNMTYQTPMIKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     SDYVFPGNQVDK"
     gene            7404..7877
                     /locus_tag="NBK22_001961"
     CDS             7404..7877
                     /locus_tag="NBK22_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCD2415144.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKTLDDAKLLDALNGAIRGRQVIINKFAVELNKDIYTGLLSNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTTIRSLLGVLKDDDMNRSLTYMLNMLKGMSREE"
     gene            8105..8338
                     /locus_tag="NBK22_001962"
     CDS             8105..8338
                     /locus_tag="NBK22_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415145.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            8360..9136
                     /locus_tag="NBK22_001963"
     CDS             8360..9136
                     /locus_tag="NBK22_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCD2415146.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVFETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLKAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK"
BASE COUNT         3315 a         1213 c         1776 g         2954 t
ORIGIN      
        1 attaaagtat tatatttaat cgtatcaatg atttgaatca aattagtaat ttgttgtctc
       61 atatacttag tttcgtttat catgtagaag tacctccaaa aatcaattat taaacaggaa
      121 aggatataac agtggaaaat gaaaaaaaga aatacacgtt taaagatata gcatggagag
      181 atttgcttat aattccaatt ataggtgtta tattttatgc actcacaatt gcagcttaca
      241 taggaccaat tttgtttggt tattatgaaa tcgatagtaa tctgatattt gtagcgacta
      301 tcacacaagc tttagcatat acgattggga ttggcgcatt ttatttattt catttaaaag
      361 tgatgccatc aaggcttcga gctggttttg cgtatatcaa aaaatattgg cttagattgt
      421 caataacgta tattattgct ctcatcttaa tttatacata tgaatatatg acacagtttc
      481 tacctaaaca tttgcaatat agtgaaacag ccaatgaact tgagctaaat aaaatgtttg
      541 aagtgcctgc atttttacct gtagcattct tattaattgt gattgtaggt ccaatagttg
      601 aagaaatcgt atttagacat attttaattg gagaattagg taagaagttt aattttatag
      661 ctatgagtat tgtttcggtg ttcttatttg catttattca tgtgactgat gcaaagtcgc
      721 cgtttgagtt tggtccatat ctcatactat ccatcatatt agttttcact tatttaaaat
      781 ctggaagaaa cctaggttca acaattgcat tacatatagc aaataatttt gtttctttcg
      841 ttatatcagt aataagtatt tatgcgtaga taaaacccca tcgactccat agtttaggaa
      901 tcgatggggt ttaaaaattg atgatagtta tcaatggata tgtctctggt ttgaatcgtc
      961 ttaccgaatg aatacgaaat atctataata ttttcaattt cgagttcggc agttttgtct
     1021 tgagcaatgt cataaatgac tgcatgccaa tgtccatgtt tacacaaaag gccaataaat
     1081 ataacgtttt cagctttaat attatcatca accctatgat ctactaacat cacatttcgt
     1141 tctgtacaat aaattaaaat gtcggaaaac agcattggga gtgtcatatg ttgtgtgtca
     1201 tcaaatttaa tataaaagtc catgcgttta agcaattttc ttaagcgtgt ttgtggaaga
     1261 cttaaacatt gttttataat tgcgttgatt tcgcttttat atggtaatgt cgagtacgac
     1321 tgactttcat taagcactaa aaataaggcc gataattgtg tttcagataa gtttaatgca
     1381 attttagatt gtgcttgttg tatttgataa ccaccatttt tcccataatg cgcataaatt
     1441 ttaacacctt gattttctaa atcatcaata tctcttaaaa ttgtgcgttt ggatacgttg
     1501 caatatttag ctaattctaa agcggtcatt tttttatttt gttgaattgc agtaattatt
     1561 aaattttgcc tttcagcttt attcatgaca gccacctcct aaaagatgat atattacaca
     1621 aagtataaca taagagtgac aatgaggaca ttttcaatat ctttttaaaa aaatcggaaa
     1681 aattttgtgg aaaacgctta cattttaaaa aagattgtgc tatgatatta cttgtaacag
     1741 ggggagcgat taaacaaagg ggtagagcta tataacagat agcttttaat cgttcaagtt
     1801 acatcacaac atcttcaata tttattactt actttccttt ctatttgtcg gctagcacgt
     1861 gactagccga tttttttatg cataaaatga agaagtcttc agttgtattg ttgattcttt
     1921 taatataggg taataataaa atgataatga tatgagaagt ggtggacgaa atgacagata
     1981 aaacattaca gcaaatagat aagttaatat gttcgtggtt gaaacaaata gacaatgtta
     2041 ttccgcaatt aattatggaa atgactacag aaactaagcg tcatcgattt gatttagtta
     2101 caaatgtaga taaacagatt caacaacaat ttcaacaatt tttagcaaca cattttccgg
     2161 aacaccaact attagcggaa gaaaaaagta atgaaatgat tacgaatgaa attaatcatt
     2221 tatggattat ggatcccatt gatggaactg ccaatttagt aaaacagcaa gaagattatt
     2281 gtattatatt ggcgtatttt tatgaaggta aaccaatgtt atcgtacgta tatgattatc
     2341 cgcataaaaa gctttataaa gcaatacgag gagaaggtgc tttttgtaat ggaattaaga
     2401 tggaagaacc accatcatta aaattggaag acgctattat atcgtttaat gcacaagtaa
     2461 tgaatctgga tacggtacaa gatttatttg acgcctcatt tagttatcgt ttagttggtg
     2521 cttgcggatt agattctatg cgagtggcaa aaggtcaatt tggggcacat attaatacaa
     2581 atcctaaacc ttgggacatt gctgcacagt ttttatttgc agaaatattg aatctaaaaa
     2641 tgacgacttt agatggtaag gcaattgatc atttaaaggg tgcacctttt attataagta
     2701 ataaagcatg tcatgaaacg gtacttaaaa ttttaaatgc aaatggtggt tatcaaaaat
     2761 acagatagat attctctaga gataaaaaat gataaaaaat agttatacgt ctacagaagt
     2821 atagtaaaaa aattatttta ctatataata aatagagaaa acagacaaag tatgtaagga
     2881 atagtaaagg agtggtggga ttgaaacgtt cgtctaaaag taaaatgagc ctaccgaaaa
     2941 aaatattttt atgggtattc ggtattttag taatcttagc gattgtggct gttgtgtatg
     3001 tagctgctaa aatttttatt actggtaata agatacacaa tccgttagat cgcaatcatt
     3061 cagaattaag agataaaaaa gtgagtttaa acgatggtga tccattcaca atcgctttat
     3121 ttggtgtcga ttcagatgct gatcgtaaga aaaagggtgg tggagagcgt agtgatagta
     3181 ttatgatttt atctatcaac cctaaaacga agaaaactga aatagtaagt ataccgcgag
     3241 atacaagagc agaaattgtt ggccgtggta ctactgaaaa aattgcacat gcctatgcat
     3301 atggtggacc aaatatggct gttaaatcac ttgaaaaatt aatgaatgtg ccaattgatc
     3361 attatgcgac aatcgatatg gatggtttgc ataatatgat agatagcatc ggcggtgttg
     3421 atgtagtaag taatgataca ttcactgttg atggtgttcg ttttacaaaa ggacagcaaa
     3481 cacatgtaaa tggtgatcaa gcattgaagt ttattagaag tcgtaaagaa gaaggtgctg
     3541 gtggtgactt tggtcgacaa caacgtcagc aaattgtgct tgaagcgatg gccaataaga
     3601 ttgcaagccc atcatcgatt acacatttta atagtctaat gaatgaaatt caaaataatg
     3661 ttaaaacaga tttaacatta ggtgatttga atacgattag aagcaattat aaagatgcta
     3721 atgacacgat taataaacat caattgagtg gtcaaggtgg cattcaaagt gacggattgt
     3781 actacttcat tcctagtgag cagtcaaaag ctgaaagtac taagttatta aaagataatc
     3841 tagagtagta aactaaggga aaataagtta tcaaaattgt acacaaatta attagataga
     3901 atgatctgta aatagaataa cgaatagaac taatagcccg taaatagaag gtgataacac
     3961 tttgtattta tgggctattt tatttaatgc ctgatttgat ttttataaat taatccatta
     4021 caaatttaat ttaaagataa atattacctt ctaaatgata gtagatattt atcaaacttg
     4081 ggttattaat tttcagaaaa agggtattta taaatagtat aacgattttt aggtgaggtg
     4141 attattaaat attgtcatta ataaggttgt gaaagatatg gcaaaaagcg tgaaacagat
     4201 aattggaaaa ctaataatga taattatttt caattaaaca aaaagatgat ttaatttcgc
     4261 gcaaaatatt tcaaataaaa atgattgtaa aaaggcaaaa tacaaaattt cacttaacaa
     4321 ctagtacata aagtaataca attaaattaa ttctatctga aagatgtgtg gggcatcgtt
     4381 attttaggtg gatatgagca atttattaaa agtcatttac ggaaaatata tatagacggg
     4441 gtgagtaata tgcaagaaca tttggtggtt acacttgatg gaaaagatta tcttgtagaa
     4501 ccaggtatga atttacttga atttattaaa tcacaagata cttttgtacc ttcaatttgt
     4561 tataacgagt cgatgggacc aattcaaaca tgtgatacat gtactgttga gattgacggt
     4621 aaaattgaac gttcatgtag tacggtgatt gatcgtccaa tgactgtaaa tactgtgaac
     4681 aatgatgtga aagatgctca aaaagaagcg cttgatcgaa ttttagaaaa gcatatgctg
     4741 tattgtacag tatgtgatta taataatggt gattgtgaaa ttcataatac gatggatgca
     4801 tgggggcttc agcatcaaac gtatgaatat aaagagaagc catacgaaaa agattacggc
     4861 ccattttatc gttatgatcc aaatcaatgt atcttatgtg ggcgttgtgt agaagcatgt
     4921 caagatatcg aagtgaatga aactattaga attgattggg atcgtgaaca tccacgtgtt
     4981 atttgggata atgatgtacc gattaatgag tcttcatgtg tatcttgtgg tcaatgtgcg
     5041 acggtatgtc catgtaatgc catgatggaa gtgaatatgg aaggtaatgc gggttatatg
     5101 actgatacgg aacctggttc attagcagca atgattgatt taactaaaaa agcagaacct
     5161 ggttatggtc cactatttgc aatatcagat tcagaagcag aaatgcgtaa agagcgtatt
     5221 aagaaaacta aaacagtgtg tacttattgt ggtgtgggtt gttcatttga agtttggact
     5281 aaagatagag aaattttaaa agtactacca tcacatgatt ctccagcaaa taaaattgcg
     5341 acttgtgtta aaggtaagtt ttcatgggga catattaatt cagatcaacg attaactaaa
     5401 ccactagtaa ggaaaaatgg tgagttccat gaagtagaat gggatgaagc tttaaacgtc
     5461 attgcagata attttacagc tattaaagaa aagcatggcc cagatgcact atcattcatt
     5521 tcttcttcta aagcgacgaa tgaagaatcg tatttaatgc aaaaattagc aagacaagtt
     5581 attggcacaa ataacgttga taactgttca agatattgcc aagcacctgc aacaaaaggc
     5641 ttatttagaa cggttggaca cggcggtgac tcaggtagta ttgaagattt agaaaaagcg
     5701 gcaatgtctg tattgatagg tactaataca gccgaagctc atccagttat cgcatcacgc
     5761 atgaaacgtg cacaaaaatt atttggtcaa aaaatacatg tgtttgatat tagaaaacat
     5821 gaaatggcag aacgtgcgga tcgtttttat caaccgaaac caggtacgga tttagcgtgg
     5881 ttaagcgcag tgactaagta tattattgat catgatttac acgataaagc atttattgat
     5941 gagtgggtag atgattttga tgaatattac aaatcattag aaacatttac aatggctttt
     6001 gctgaagaag caacaggtat tcctaaatca gaattgatta aatttgctga agaatgtgct
     6061 aaagctgaat ctgttgtaat ttgttgggca atgggtatta cacaacaaga cattggtagt
     6121 gactcaagta cagcgatttc aaacttatta ttagtaacag gtaattatcg tcgtcctggt
     6181 actggtgcgt atccattacg tggacataat aatgttcaag gatgtagtga tatgggaagt
     6241 atgcctgata agattactgg ttatcaaagt attgaagcgg atgatattcg cgctaaattt
     6301 gaaaaagaat atggcgttaa attgaatcca aaagctggta aagataatca tgaaatggta
     6361 gaaggtatac atgatggaga agtacactca ttgtacttat atggtgaaga tacgggtatt
     6421 gtagattcaa atattaattt tgtacaagct gcgtttgaaa aattagattt catggtagtc
     6481 caagatgaat ttttaacatt cacagcaaca tacgcagatg ttgtattgcc agcaagtcct
     6541 tcacttgaaa aagacggtac ttttacaaat accgaacgtc gtattcaacg tttataccaa
     6601 gcattagaac ctcttggtga ttcaaaacct gactggaaaa ttttccaagc aattgctaat
     6661 agattaggat ttgattggaa ttacaagcat cctagtgaaa ttatggatga agtagcacgc
     6721 ttaacacctc tatatgctgg ggtaagttat gatcgtttag aaggattcaa tagtttacaa
     6781 tggccagtac aacctgatgg tactgatgag cctattctat acttagaagg attcaatttt
     6841 gataatggta aagcaaaatt attcccatta tcatttgata attactttaa gcaagatgaa
     6901 atttatgata ttcatgtaaa caatggtcga ttgttagagc acttccatga aggtaatatg
     6961 acttatcaaa caccaatgat taaatacaaa gtgccacgtg catttgttga aatttctcca
     7021 gagcttgctg aagatagagg cattcatgaa ggtgcagaag ttaagctgat ttctgaaaca
     7081 ggagaagcgg tattacaagt tcacgtgaca gatcgtgtta aaggtaaaga aatctatatt
     7141 ccattaaata atgatgcgat ggaaaatgga gatctaggtg caattaactt attaactaat
     7201 agtgatgttg atcaatacac ggatacacca tcttataaac gtactagctg tcgtttggag
     7261 gtaattacga aacgtggtaa gtcaccgttg aaccctaata atttccgtgt caataaaaaa
     7321 cgtcatccgc agtacagtgt tcaagtacag aaaaaatggg aacgttcaga ttatgttttc
     7381 ccgggaaatc aggtggataa ataatggctg agagaatatc ttctaaaatt agacgcttag
     7441 aaaaatcaga agaacaaatt aagttagaaa gtttaaatga agttacagag gcaattgcag
     7501 cgaataaaga tagtatatta aaagcaatca agcttattaa aacattagat gatgcaaaac
     7561 tattagatgc tttaaatggt gcaattcgag gacgtcaagt catcataaat aaatttgcag
     7621 ttgaacttaa taaagatatt tatacagggc tattatctaa tatggcttca atggtatttt
     7681 tattaggtga actcaatgta tcagatttaa gcgacttcct aaataaagtt aataaaggat
     7741 tacacgttgc caatcaagcg agtccaaatg ctaagacaac tataagaagt ctattgggcg
     7801 tattgaaaga tgacgacatg aaccgtagtt taacatacat gcttaatatg ctaaaaggta
     7861 tgtctcgaga agaataacat tacaatgaaa aagtgtttct acatgcaagc agtgtaggag
     7921 cacttttttt aatttttgta aaaattaaaa agggtgtttt tgatggaata aatgaataat
     7981 attgaaaaac aaaaaaatca cgtgctattg tgaatcaatc atcgacaggc ctatgaaaca
     8041 tcatgtatta ttatgattat gtaagttatt taaataaaaa gggtgagtgc atattaattc
     8101 gagaatgtta aaaacgtcag ctgtaatttc taatacatta ttagtcatag gtttagtctg
     8161 tctatttatg atgaaattag tgttagctat tacttttttt gctgtttcat tatcaattag
     8221 tctagttgtc tttaatatga tgttcagaga tagaacaacg atgaaagtaa tcgttaacgc
     8281 ttctttttta atcgtaatat tagcgattgt agtggcatat tttttattgt ctaagtaaga
     8341 aaaatgaggt tttgtctata tgaagaagaa tttcaagtta cgcatttcaa cgctactatt
     8401 gatagttatt ttagttgttt ttgctgtatt actcatcgtg tttgaaacta aattgtttaa
     8461 aaatgatgtg aattactctt ttgatgaggc tgtttcaatg caacaaggga aaggtattgt
     8521 acagacaaaa gaagaggatg gtaaatttgt tgaagcaaat aataatgaaa ttgctaaagc
     8581 aatgactatt tcacataaag acaatgatat gaagtatatg gatatcacag aaaaagtgcc
     8641 aatgtcggaa tctgaagtta accaattgct aaaaggtaag gggattttag aaaatcgagg
     8701 gaaagttttt ctaaaagctc aagaaaaata tgaggttaat gtcatttatc ttgttagcca
     8761 tgcattagta gaaacaggta acggcaaatc agaattagca aaaggaatta aagatggaaa
     8821 aaaacgctat tacaactttt ttggtatagg agcattcgat agtagtgctg ttcgtagtgg
     8881 gaaaagttat gctgaaaagg aacaatggac atcaccagat aaggcgatta ttggtggtgc
     8941 aaagttcatt cgtaatgaat attttgaaaa caatcaactg aatttatatc aaatgcgatg
     9001 gaatccagaa aatcctgcgc aacatcaata tgcgagtgac attcgctggg cagataaaat
     9061 tgccaaatta atggataaat cctataagca gtttggtata aagaaagatg atattagaca
     9121 aacatattat aaataagaca tcggtgctta aaggagctgg aacaatttat tgtttcgagc
     9181 tcctttagcg cattctgagt gtgttagtta aatggatttt aacctaacaa aaaacgcggg
     9241 agtgggacag aaatgata
//
LOCUS       DAHHYJ010000107         9240 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000107 DAHHYJ010000000
VERSION     DAHHYJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.107"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            83..490
                     /locus_tag="NBK22_001964"
     CDS             83..490
                     /locus_tag="NBK22_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415147.1"
                     /translation="MSSIIGKIAIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILALFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSSILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            540..1166
                     /locus_tag="NBK22_001965"
     CDS             540..1166
                     /locus_tag="NBK22_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2415148.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTCKKELGSQDMM
                     EHPEVKNPTTKNLEGFNEEIHKDEATTKVVSDNTEPPIESKDHVSKKD"
     gene            1378..2154
                     /locus_tag="NBK22_001966"
     CDS             1378..2154
                     /locus_tag="NBK22_001966"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCD2415149.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGNPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            2298..2420
                     /locus_tag="NBK22_001967"
     CDS             2298..2420
                     /locus_tag="NBK22_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415150.1"
                     /translation="MHQLKALLVLTHPRYYKTSQKHHYLIYLNKNSQSYLILFL"
     gene            2490..3470
                     /locus_tag="NBK22_001968"
     CDS             2490..3470
                     /locus_tag="NBK22_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD2415151.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS"
     gene            3433..4125
                     /locus_tag="NBK22_001969"
     CDS             3433..4125
                     /locus_tag="NBK22_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD2415152.1"
                     /translation="MKKLQLKKWKCRENDIEAVHNNKGYAYQRKLDKLEEVRKSYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKFIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            4335..5501
                     /locus_tag="NBK22_001970"
     CDS             4335..5501
                     /locus_tag="NBK22_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCD2415153.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDERVNVIIMEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSRITDSIICVSDFDKQLALKY
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGKVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVAQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVADNGICMM
                     NNQPETIAKVLEKYLIASDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            5482..6720
                     /locus_tag="NBK22_001971"
     CDS             5482..6720
                     /locus_tag="NBK22_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCD2415154.1"
                     /translation="MENQHNSKLLTLLLIGLAVFIQQSSVIAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            6710..8140
                     /locus_tag="NBK22_001972"
     CDS             6710..8140
                     /locus_tag="NBK22_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCD2415155.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLFLALTVFAMYM
                     VVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSIQQ
                     SHSLTKTLANNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVINLVLCIILIYFYGIYGAAIARLITEFI
                     LLIWRFVDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
     gene            8408..9007
                     /locus_tag="NBK22_001973"
     CDS             8408..9007
                     /locus_tag="NBK22_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD2415156.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT         3163 a         1269 c         1674 g         3134 t
ORIGIN      
        1 tttttgtatt ttgcatataa aaattaaata aaataaggta tattaaggta aagtataaat
       61 tttaaataaa tggggaatga gtatgagctc aattatagga aaaatagcaa tttggatagg
      121 catcgtagct caaatatatt ttagtgtcgt ttttgttagg atgatatcta ttaatattgc
      181 tggaggatct gattacgaaa caattttttt attaggatta atattggctc ttttcactgt
      241 tttaccaacc atctttactg cgatttatat ggaaagttac tctgtaatcg gaggtgcact
      301 ttttattgtt tatgctatta ttgcactgtg tttatataat ttcctttcgt caattttatg
      361 gctgattggt ggtattttgc tgatttggaa taaatactca aaagatgaat cgacagacga
      421 aaatgaaaaa gttgatattg aaagtacaga gaatcaattt gaatctaaag ataaaatcac
      481 taaagaataa agagaatatt taaggtaaag tataaatttt aaataaatgg ggaatagaca
      541 tggaaaaaaa tgtagaaaaa tcattcataa agataggttt atattttcaa atagcttata
      601 tagtactcat ggctataact ttatgtgggt ttgtaatttg ctatggacta attttcggcc
      661 ttttctattt attatcaggt agcagagctg attatttaat agtaacaata gttatatcgg
      721 caataatttc tatatttgta attatacttt caatcgtacc tgtcatcgta ttggcatctg
      781 acttatttaa agaaaggatt tcaaaaggtg tcatattaat tgtattggct attattgctt
      841 tagtattatg caactttgta tctgcaatac tctggtttgt ttcagccata tctattttag
      901 gtagaaaaaa attagtagct gcagcagata ctaccactat tcaaaaaagt aaagggaacg
      961 caaatcaagc atcacataaa gacacgtgta aaaaggaact tggtagtcaa gacatgatgg
     1021 aacatcctga ggttaaaaat cccacgacta aaaaccttga aggatttaac gaagaaatac
     1081 ataaagatga agctacaact aaagttgtca gtgataacac ggaaccgccg attgaatcaa
     1141 aagaccatgt ctcgaaaaaa gattgatgac aaactaatcg agagacttaa aaaaataata
     1201 ttcaacataa gcacttttaa aacgacattt aaacgcattg ccaatcacta atggtagtgc
     1261 gtttaactat accttaaata tctgaatatt ttgttaaatg gagctacctt tgttgtacta
     1321 ttcaaatgaa gaggagtaaa atgtaattaa aggaaagaaa tttgaggagt gatctttatg
     1381 acaaacaaca aagtagcatt agtaactggc ggagcacaag ggattggttt taaaattgca
     1441 gaacgtttag tggaagatgg tttcaaagta gcagttgttg atttcaatga agaaggggca
     1501 aaagcagctg cacttaaatt atcaagtgat ggtacaaaag ctattgctat caaagcagat
     1561 gtatcaaacc gtgatgatgt atttaacgca gtaagacaaa ctgccgcgca atttggcgat
     1621 ttccatgtca tggttaacaa tgccggcctt ggaccaacaa caccaatcga tacaattact
     1681 gaagaacagt ttaaaacagt atatggcgtg aacgttgcag gtgtgctatg gggtattcaa
     1741 gccgcacatg aacaatttaa aaaattcaat catggcggta aaattatcaa tgcaacatct
     1801 caagcaggcg ttgagggtaa cccaggcctg tctttatatt gcagtacaaa attcgcagtg
     1861 cgaggtttaa cacaagtagc cgcacaagat ttagcgtctg aaggtattac tgtgaatgca
     1921 ttcgcacctg gtatcgttca aacaccaatg atggaaagta tcgcagtggc aacagccgaa
     1981 gaagcaggta aacctgaagc atggggttgg gaacaattta caagtcagat tgctttgggc
     2041 agagtttctc aaccagaaga tgtttcaaat gtagtgagct tcttagctgg taaagactct
     2101 gattacatta ctggacaaac aattattgta gatggtggta tgagattccg ttaataatca
     2161 tgcattaacg ataaataaat ccttattgtt aagtttaatc acttagcagt aaggattttt
     2221 tagtgcactt agaagggatt gtattggtag aaaattaata agcgaagttc ttaaatgagt
     2281 tatgatgtca cagtctaatg catcagttga aagcattact agtattaaca cacccaagat
     2341 attataaaac atcacaaaaa caccactatc taatttatct caataaaaat tcacaaagtt
     2401 atctcatttt atttttataa ataaaaaata tcgataaaaa gcttacaata ctttatgttt
     2461 ttatgatata tttttaatgt ataaatgagg tggaagattt ggaaagagtt ttgataactg
     2521 gtggggctgg ttttattggg tcgcatttag tagatgattt acaacaagat tatgatgttt
     2581 atgttctaga taactataga acaggtaaac gagaaaatat taaaagtttg gctgacgatc
     2641 atgtgtttga attagatatt cgtgaatatg atgcagttga acaaatcatg aagacatatc
     2701 aatttgatta tgttattcat ttagcagcat tagttagtgt tgctgagtcg gttgagaaac
     2761 ctatcttatc tcaagaaata aacgtcgtag caacattaag attgttagaa atcattaaaa
     2821 aatataatag tcatataaaa cgttttatct ttgcttcgtc agcagctgtt tatggtgatc
     2881 ttcctgattt gcctaaaagt gatcaatcat taatcttacc attatcacca tatgcaatag
     2941 ataaatatta cggcgaacgg acgacattaa attattgttc gttatataac ataccaacag
     3001 cggttgttaa attttttaat gtatttgggc caagacagga tcctaagtca caatattcag
     3061 gtgtgatttc aaagatgttc gattcatttg agcataacaa gccatttaca ttttttggtg
     3121 acggactgca aactagagat tttgtatatg tatatgatgt tgttcaatct gtacgcttaa
     3181 ttatggaaca caaagatgca attggacacg gttataacat tggtacaggc acttttacta
     3241 atttattaga ggtttatcgt attattggtg aattatatgg aaaatcagtc gagcatgaat
     3301 ttaaagaagc acgaaaagga gatattaagc attcttatgc agatatttct aacttaaagg
     3361 cattaggatt tgttcctaaa tatacagtag aaacaggttt aaaggattac tttaattttg
     3421 aggtagataa tattgaagaa gttacagcta aagaagtgga aatgtcgtga aaatgacatt
     3481 gaagctgtcc ataataataa gggttatgcc tatcaaagaa aattagacaa actagaagaa
     3541 gtgagaaaaa gctattaccc aattaaacgt gcgattgact taattttaag cattgtttta
     3601 ttatttttaa ctttaccgat tatggttata ttcgccattg ctatcgtcat agactcacca
     3661 ggaaacccta tttatagtca ggttagagtt gggaagatgg gtaaattaat taaaatatac
     3721 aaattacgtt cgatgtgtaa aaacgcagag aaaaatggtg cgcaatgggc tgataaagat
     3781 gatgatcgta taacaaatgt cgggaagttt attcgtaaaa cacgcattga tgaattacca
     3841 caactaatta atgttgttaa aggggaaatg agttttattg gaccacgccc ggaacgtccg
     3901 gaatttgtag aattatttag ttcagaagtg ataggtttcg agcaaagatg tcttgttaca
     3961 ccagggttaa caggacttgc gcaaattcaa ggtggatatg acttaacacc gcaacaaaaa
     4021 ctgaaatatg acatgaaata tatacataaa ggtagtttaa tgatggaact atatatatca
     4081 attagaacat tgatggttgt tattacaggg gaaggatcaa ggtagtctta atttacttaa
     4141 taagttcaaa taaaagttat attttaaaga ttgtgaccaa ttgttacagt ataacgagga
     4201 atcccttgag acagtatgaa atggcattaa gaaatatgtg ccatcattga tttgcatggc
     4261 tataaatact attcatctga tgagatagcc atgttaagaa attgaaagta tagcattaaa
     4321 ggggtttgta acagttgaaa attatatatt gtattactaa agcagacaat ggtggtgcac
     4381 aaacacatct cattcaactc gccaaccatt tttgcgtaca ccatgatgtt tatgtcattg
     4441 taggcaatca tggaccaatg attgaacaac tagatgaaag agttaatgta attattatgg
     4501 aacatttagt aggtccaatt gactttaaac aagatatttt agctgtcaaa gtgttagcac
     4561 agttattctc gaaaattaag cctgatgtta tccatttaca ttcttccaaa gctggaacgg
     4621 tcggacgaat tgcgaagttc atttcgaaat cgaaagacac acgtgtagtt tttactgcgc
     4681 atggatgggc ttttacagag ggtgttaaac cagctaaaaa atttctatat ttagttatcg
     4741 aaaaattaat gtcacgtatt acagatagca ttatttgtgt ttcagatttc gataaacagt
     4801 tagcgttaaa atatcgattt aatcgattga aattaaccac aatacataat ggtattgcag
     4861 atgttcccgc tgttaagcaa acgctaaaaa gccaatcaca taacaatatt ggcaaagtag
     4921 ttggaatgtt gcctaataaa caagatttac agattaatgc cccgacaaag catcaatttg
     4981 ttatgattgc aagatttgct tatccaaaat tgccacaaaa tctaatcgcg gcaatagaga
     5041 tattgaaatt acataacagt aatcatgcgc attttacatt tataggcgat ggacctacat
     5101 taaatgattg tcagcaacaa gttgcacaag ctgggttaga aaatgatgtc acatttttgg
     5161 gcaatgtcat taatgcgagt catttattat cacaatacga tacgtttatt ttaataagta
     5221 agcatgaagg tttgccaatt agcattatag aagctatggc tacaggtttg cctgttatag
     5281 ccagtcatgt tggcggtatt tcagaattag tagctgataa tggtatatgt atgatgaaca
     5341 atcaaccaga aactattgcc aaagtcttgg aaaaatattt aatagccagt gattacatca
     5401 aaatgagtaa tcaatctaga aaacgttatt tagaatgttt tactgaggag aaaatgatta
     5461 aagaagtgga agacgtttat aatggaaaat caacacaata gtaaattact aacattgtta
     5521 cttatcggtt tagcggtttt tattcagcaa tcttcggtta ttgccggtgt gaatgtttct
     5581 atagctgact ttatcacatt actaatatta gtttatttac tgtttttcgc taaccattta
     5641 ttaaaggcaa atcatttttt acagtttttc attattttgt atacatatcg tatgattatt
     5701 acgctttgtt tgctattttt tgatgatttg atatttatta cggttaagga agttcttgca
     5761 tctacagtta aatatgcatt tgtagtcatt tatttctatt tagggatgat catctttaag
     5821 ttaggtaata gcaaaaaagt gatcgttacc tcttatatta taagcagtgt gactataggt
     5881 ctattttgta ttatagctgg tttgaacaag tcccctttac taatgaaatt gttatatttt
     5941 gatgaaatac gttcaaaagg attaatgaat gaccctaact atttcgcgat gacacagatt
     6001 attacattgg tacttgctta caagtatatt cataattaca tattcaaggt ccttgcatgt
     6061 ggtattttac tatggtcttt aactacaacg gggtctaaga ctgcgtttat catattaatc
     6121 gtcttagcca tttatttctt tattaaaaag ttatttagca gaaatgcggt aagtgttgtg
     6181 agtatgttag tgattatgct gatattactt tgttttactt tttataatat caactactat
     6241 ttattccaat taagcgacct tgatgcctta ccgtcattag atcgaatggc gtctattttt
     6301 gaagaggggt ttgcatcatt aaatgatagt ggatctgagc gaagtgttgt atggataaat
     6361 gccatttcag taattaaata tacactaggt tttggtgtcg gattagtgga ttatgtacat
     6421 attggctcgc aaattaatgg tattttactt gttgcccata atacatattt gcagattttt
     6481 gcggaatggg gcattttatt cggtgcatta tttatcatat ttatgcttta tttactgttt
     6541 gaattattta gatttaacat ttctgggaaa aatgtaacag caattgttgt aatgttgacg
     6601 atgctgattt actttttaac agtatcattt aataactcaa gatatgtcgc ttttatttta
     6661 ggaattatcg tctttattgt tcaatatgaa aagatggaaa gggatcgtaa tgaagagtga
     6721 ttcactaaaa gaaaatatta tttatcaagg gctataccaa ttgattagaa cgatgacacc
     6781 actgattaca atacccatta tttcacgtgc atttggtccc agtggtgtgg gtattgtttc
     6841 attttctttc aatatcgtgc aatacttttt gatgattgca agtgttggcg ttcagttata
     6901 ttttaataga gttatcgcga agtccgttaa cgacaaacgg caattgtcac agcagttttg
     6961 ggatatattt gtcagtaaat tatttttagc gttaacagtt tttgcgatgt atatggtcgt
     7021 aattactata tttattgatg attactatct tattttccta ctacaaggaa tctatattat
     7081 aggtgcagca ctcgatattt catggtttta tgctggaact gaaaagttta aaattcctag
     7141 cctcagtaat attgttgcgt ctggtattgt attaagtgta gttgttatct ttgtcaaaga
     7201 tcaatcagat ttatcattgt atgtatttac tattgctatt gtgacggtat taaaccaatt
     7261 acctttgttt atctatttaa aacgatacat tagctttgtt tcggttaatt ggatacacgt
     7321 ctggcaattg tttcgttcgt cattagcata cttattacca aatggacagc tcaacttata
     7381 tactagtatt tcttgcgttg ttcttggttt agtaggcaca taccaacaag ttggtatctt
     7441 ttctaacgca tttaatattt taacggtcgc aatcataatg attaatacat ttgatcttgt
     7501 aatgattccg cgtattacca aaatgtctat ccagcaatca catagtttaa ctaaaacgtt
     7561 agctaataat atgaatattc aattgatatt aacaatacct atggtctttg gtttaattgc
     7621 aattatgcca tcattttatt tatggttctt tggtgaggaa ttcgcatcaa ctgtcccatt
     7681 gatgaccatt ttagcgatac ttgtattaat cattccttta aatatgttga taagcaggca
     7741 atatttatta atagtgaata aaataagatt atataatgcg tcaattacta ttggtgcggt
     7801 gataaaccta gtattatgta ttattttgat atatttttat ggaatttacg gtgctgctat
     7861 tgcacgttta attacagagt ttatcttact tatttggcga tttgttgata ttactaaaat
     7921 caatgtgaag ttgaatattg taagtacgat tcaatgtgtc attgctgccg ttatgatgtt
     7981 tattgtgctt ggtgtggtca atcattattt gccccctaca atgtacgcta cgctgctatt
     8041 aattgcgatt ggtatagtag tttatctttt attaatgatg actatgaaaa atcaatacgt
     8101 atggcaaata ttgaggcatc ttcgacataa aacaatttaa gtaccggtaa tgctatactt
     8161 tagaaaatta agattaagaa gaaaaggcaa tttcttattg aaaaatggaa gttgtctttt
     8221 ttaattcttt ttaaaagcgg gaaacaaaag cagttaaatg cctttttgca ttcaatatta
     8281 aatattatat caatttcgaa tatttaaatt ttatataatt ggatataaca aataaataat
     8341 aattattgca aaacacaccc aaaattaatt attataaaag tatattcata aaaggaggaa
     8401 tatacttatg gcatttaaat taccaaattt accatatgca tatgatgcat tggaaccata
     8461 tatagatcaa agaacaatgg agtttcatca cgacaaacat cacaatacgt acgtgacgaa
     8521 attaaacgca acagttgaag gaacagagtt agagcatcaa tcactagcgg atatgattgc
     8581 taacttagac aaggtaccgg aagcgatgag gatgtcagtc cgtaataatg gcggtggtca
     8641 ttttaaccat tcattattct gggaaatact atcacctaat tctgaagaaa aaggtggcgt
     8701 aatagatgac atcaaagcgc agtggggcac tttagatgaa tttaaaaatg aatttgcaaa
     8761 taaagcaaca acattatttg gatcaggttg gacttggtta gttgttaatg atggcaaatt
     8821 agaaattgtg acaacgccaa accaagataa tccattaaca gaaggcaaaa caccaatctt
     8881 actatttgat gtttgggagc atgcctacta tctgaaatat caaaataaac gtccagacta
     8941 tatgactgca ttttggaata ttgttaactg gaaaaaagtt gatgaattat accaagcagc
     9001 aaaataatat aacttaatat aattgaggtg gagcatctac aaggtgttcc acttttttgt
     9061 acttaatatc tttacttatt tgtttttgtg taatgggata gcacgtaaaa gtggcaaaat
     9121 aataagataa atttcaaact cgaaatacct tattattgtt gactaaaacg atgacttaaa
     9181 tgttatatac attagacaac actattaatt aatcaagata ttgtcattaa ataaattgac
//
LOCUS       DAHHYJ010000108         9121 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000108 DAHHYJ010000000
VERSION     DAHHYJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.108"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(81..683)
                     /locus_tag="NBK22_001974"
     CDS             complement(81..683)
                     /locus_tag="NBK22_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415157.1"
                     /translation="MLISLVTPVFALLVIGGIIWVIIEGIVHISKKNKAIDNFLNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSALLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNIMER"
     gene            complement(892..1188)
                     /locus_tag="NBK22_001975"
     CDS             complement(892..1188)
                     /locus_tag="NBK22_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCD2415158.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKTRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSARHQFQDNTLVSPYPGAKEMILIPDFDEATNLTDLFKTIKNDLKN"
     gene            complement(1237..1440)
                     /locus_tag="NBK22_001976"
     CDS             complement(1237..1440)
                     /locus_tag="NBK22_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415159.1"
                     /translation="MEDLKHSLKSLGWWDLFFAIPMFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSNTK"
     gene            1749..2735
                     /locus_tag="NBK22_001977"
     CDS             1749..2735
                     /locus_tag="NBK22_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCD2415160.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYSIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDIKIEL"
     gene            complement(3401..4594)
                     /gene="sqr"
                     /locus_tag="NBK22_001978"
     CDS             complement(3401..4594)
                     /gene="sqr"
                     /locus_tag="NBK22_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II sulfide:quinone oxidoreductase Sqr"
                     /protein_id="HCD2415161.1"
                     /translation="MNKHYQIVIIGGGTAGVTVASRLLRKNQNLKEKIAIIDPADHHY
                     YQPLWTLVGAGVSSLKSSRKDMESVIPEGAHWIKQAVSSFQPENNSVILGDNTVVYYD
                     FLVVAPGLQINWSSIKGLKENIGKNGVCSNYSPDYVNETWNQISNFKQGNAIFTHPNT
                     PIKCGGAPMKIMYLAEDYFRKHKIRSNANVIYATPKDALFDVGKYNKELERIVEERNI
                     TVNYNYNLVEIDGDKKVATFEHIKTYDRKTISYDMLHVTPPMGPLDVVKESTLSDSEG
                     WVDVNPTTLQHKSYSNVFALGDASNVPTSKTGAAIRKQAPIVANNLLQVMNNQMLTHH
                     YDGYTSCPIVTGYNRLILAEFDYNKNTKETMPFNQAKERRSMYIFKKDLLPKMYWYGM
                     LKGLI"
     gene            complement(4613..5947)
                     /gene="cstB"
                     /locus_tag="NBK22_001979"
     CDS             complement(4613..5947)
                     /gene="cstB"
                     /locus_tag="NBK22_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide dioxygenase-sulfurtransferase CstB"
                     /protein_id="HCD2415162.1"
                     /translation="MFFKQFYDNHLSQASYLVGCQRTGEAIIIDPVRDLSKYIEVADS
                     EGLTITQATETHIHADFASGIRDVAKRLNANIYVSGEGEDALGYKNMPSKTQFVKHGD
                     IIQVGNVKLEVLHTPGHTPESISFLLTDLGGGSSVPMGLFSGDFIFVGDIGRPDLLEK
                     SVQIKGSTEISAKQMYESVQNIKNLPDYVQIWPGHGAGSPCGKALGAIPISTIGYEKI
                     NNWAFNEIDETKFIESLTSNQPAPPHHFAQMKQVNQFGMNLYQSYDVYPSLDNKRVAF
                     DLRSKEAFHGGHTKGTINIPYNKNFINQIGWYLDFEKDIDLIGDKSTVEKAKHTLQLI
                     GFDKVAGYRLPKSGISTQSIHSADMTGKEEHVLDVRNDEEWNNGHLYQAVNIPHGKLL
                     NENIPFNKEDKIYVHCQSGVRSSIAVGILESKGFENVVNIREGYQDFPESLK"
     gene            complement(5978..7045)
                     /gene="cstA"
                     /locus_tag="NBK22_001980"
     CDS             complement(5978..7045)
                     /gene="cstA"
                     /locus_tag="NBK22_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide response sulfurtransferase CstA"
                     /protein_id="HCD2415163.1"
                     /translation="MKQYGEKFIDEFSKAELEKLAKQGQLIDVRTEEEYALGHINGSI
                     LHPVDEIESFNKEKNKTYYVICRSGNRSANASKYLAKQGYNVINLDGGYKAYEEENDS
                     YDTQEEYKSIEIKADRKQFNYRGLQCPGPIVKISQEMKNIEVGDQIEVKVTDPGFPSD
                     IKSWVKQTRHTLVKLDENDNGINAIIQKEKAKDLDINYSAKGTTIVLFSGELDKAVAA
                     LIIANGARAAGKDVTIFFTFWGLNALKKVQTVNVKKQGIAKMFDLMLPKKNIRMPLSK
                     MNMFGLGNMMMRYVMKKKNVDSLPTLINQAIEQNIKLIACTMSMDVMGIQKEELRDEV
                     EYGGVGTYIGATENANHNLFI"
     gene            7182..7442
                     /gene="cstR"
                     /locus_tag="NBK22_001981"
     CDS             7182..7442
                     /gene="cstR"
                     /locus_tag="NBK22_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="persulfide-sensing transcriptional repressor
                     CstR"
                     /protein_id="HCD2415164.1"
                     /translation="MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSS
                     LQRLMSIIISENLIECVKAAADDEESSQELINEAVNLLVKSK"
     gene            7442..8197
                     /locus_tag="NBK22_001982"
     CDS             7442..8197
                     /locus_tag="NBK22_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCD2415165.1"
                     /translation="MDIVNIVIMLLIGVFGGFISGLVGVGGAIIIYPAILLLPPLFGA
                     PAYSAYIASGLTSSQVFFSTLSGSLKARKKTEFSPQLVLYMGGGMIIGSMLGAFLANL
                     FDAIFVNTVYIIIALLALTLMFIKVKPSSEKSSFNKYLLVIIGLFIGIISGIVGAGGA
                     FIIIPILLVLFKLPMNTVVANSIVIAFISSIGAFVIKLIQGYIPLYDALFLIIGSIIF
                     APIGLKLSKKVPNIIQKWIISILIVFAIVQLIL"
     gene            8295..8396
                     /locus_tag="NBK22_001983"
     CDS             8295..8396
                     /locus_tag="NBK22_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415166.1"
                     /translation="MMKYPYMLININLSTITKNDKRFNATDDRINII"
     gene            8412..8864
                     /locus_tag="NBK22_001984"
                     /pseudo
     CDS             8412..8864
                     /locus_tag="NBK22_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT         3035 a         1535 c         1353 g         3198 t
ORIGIN      
        1 ataaaaattt cttctatgcc ttcatttcca actaaatttg aacatcaaaa aagagcactc
       61 ctgaatgagc actcccttaa ttatctttcc attatatttg tattgtcgac ggtttcgatt
      121 tctttatttt tatttgtagc ttttttcgca acaattaaca acgttgaaag tactgctaaa
      181 gctaaataag cataaccgcc cgcttgtttc attacaaaac taccgtaata gtatcttacg
      241 ccaccttcgt ccatataaat tttcgtagtg tgaaccaaaa ctagagataa gcctagacaa
      301 attgacaaac caatcgtagt gtaaaccatg tattttttaa atgtctgttt tttctttttt
      361 tgcttataaa acggtagaaa acataatcca tatggcatta aaaaaatgat acctgcaata
      421 ccaaaggtaa gtatgacaag ccaaaaaagt aacgctgata ctatataata tagataaaat
      481 tgagaaatac ccgccgtagc caagattaga aataaaaaag tagtagcgaa tttatatgtc
      541 tcacttactt tattaacttg gtttaaaaag ttatcgattg ctttattctt ttttgaaata
      601 tgtactatac cttctataat cacccaaata ataccaccaa taactaacaa agcaaaaact
      661 ggggttacga gactaattaa catatcgttc acctgctcac ctctattttc aaataattgt
      721 gattgaatta aatcattcta atttttgtaa agttacttat tcaatttatt atatttatta
      781 actctatgat aacaaaaata ctgtttgtgt ataacaataa atcgagatac tcaataaatt
      841 gacaatatga aattgatgat tttcaaaata gttgagtatg gcaattaatg attagttttt
      901 caaatcattt tttatggtct taaataaatc agtgagattt gttgcttcgt caaaatctgg
      961 aattaatatc atctctttag caccaggata cggcgatact aatgtattat cttgaaattg
     1021 atgacgtgca cttttagtcg ccttgaccaa taatctatta tcatacaaac cacctataac
     1081 aacaccatca taataaataa tatattctcc catcatcttt ctagttttaa cggcatttga
     1141 attcacatga tttaaaaata aatcatgtac atctttctta gtcgccattg taatcgctcc
     1201 ttcagtttta tgtttaatca cattcgtatt aatgattcat ttcgtgttgc tcttaatttt
     1261 atctataatt atatgcgtag ttaaaatcaa acctatggaa aagaaaataa tgataacaat
     1321 gttaagaaat atcgttataa aattatagtt tggaaggtat gcgaatagta gaaacatagg
     1381 tatcgcaaaa aataaatccc accaacctaa actttttaaa gaatgcttta aatcttccat
     1441 aatatcacct ttataaattt gtctttgtta taagataact aaaaaatcgc tttactgtaa
     1501 aagtagccaa agaaaattct gaatcatatt cataagtagt gtatcattaa taatgaacta
     1561 tctaatacga taatccttga tctttgtatt gatcaactta ccacaacatt cattttagac
     1621 tactcttaga cttccctttc aaatggttgc atctattgaa attccttttg tataagttag
     1681 gcttttgtgg taatatcatc atgcataaaa aatcgagata ctaattataa agagggtata
     1741 aatatattat gaaagaaaat ttttggagtg aattaccacg tccatttttt attttggcgc
     1801 caatggaaga cgttacagat atcgtctttc gacacgttgt aagtgaagca gctagaccgg
     1861 atgtgttttt cactgaattt acaaatactg aaagcttttg ccaccctgaa ggcatacata
     1921 gtgtgcgcgg acgcttaact tttagtgaag atgaacagcc gatggtcgct catatatggg
     1981 gagataagcc agaacagttc cgtgaaacga gtattcaatt agctaaaatg ggctttaaag
     2041 gcatagactt aaatatggga tgtcctgtag caaatgttgc taaaaagggt aagggttccg
     2101 gtttaatctt aagaccggat gtcgctgcgg aaattattca agcaactaaa gcaggtgggc
     2161 ttccggtaag tgttaaaaca cgccttggct actatgaaat cgatgaatgg aaagattggt
     2221 tgaagcacgt cttcgaacaa gatattgcca atttatctat tcatcttcgt acacgtaaag
     2281 aaatgagtaa agtagatgca cattgggaat taatcgaagc tattaaaaat ttacgtgatg
     2341 aaattgcacc aaacacattg ttaacaatta acggtgatat tcccgataga aaaacaggac
     2401 ttgaacttgc agaaaaatat agcattgatg gcgtcatgat tggtagaggc attttccata
     2461 atccattcgc ttttgaaaaa gaaccacgtg aacacacaag caaagaacta ttagatctgt
     2521 tgagattgca tctatcattg tttaacaaat atgagaaaga tgaaatacga caattcaaga
     2581 gcttgcgtag attctttaaa atctatgtgc gtggcataag aggcgctagc gaacttcgcc
     2641 atcaattgat gaacacacaa tcaattgcag aagcacgagc actactagat gaatttgaag
     2701 cccaaatgga cgaagacatt aaaatagaat tatagtatga gtaataaagg ttgaggatga
     2761 tatttcccaa tttaacacgg attgaacact ttctacttag gtattatctg gttttcctga
     2821 taggataact cccgatgatg cctttttact tatatcactc atccttttgg aaatggtgca
     2881 aaattaaaaa tacatcagga tatcgtttaa ctcagaaaaa aactccaatt aaaaaattgc
     2941 ttacaacaca ttatgaaatt aaaattgctc ttagtgttca acagacaaag ttgttgaaat
     3001 tactattgga ggtacatacc aagctatcat tttgagtaca cgtggtttga atatctggca
     3061 agaaatggat attggtaatg tcagtcataa agtcgctaaa cttgctcaaa taccattaat
     3121 tattattaaa taatttagag gatgaggtat aaacctttaa aaaaacagca gtgagatgat
     3181 tttcaattag aaaacatctt actgctgttt ctattttcat aatcattctt attgaatgac
     3241 ttcatcgtga tcatttaaaa cagtaacaac tttttcaatc ttttactatt tagagctaaa
     3301 aattcacgac atataaacac aacactgaca cgtcatttaa atacttaata ttttatagta
     3361 aaagattttt cattaaaaat ttattgtttt ctgtacttta ttatattaat ccttttagca
     3421 tgccgtacca atacatttta ggtaataaat ctttcttaaa tatatacata cttctacgtt
     3481 ctttggcctg attaaacggc attgtttctt tagtattttt attataatca aactctgcaa
     3541 gtattaacct attatatcca gtaacaatag gacatgaagt ataaccatca taatgatgcg
     3601 ttaacatttg attattcatc acttgcaata aattattagc gacgataggt gcttgcttac
     3661 gaatagctgc gcctgttttt gaagtaggta catttgaagc atcaccaagt gcaaatacat
     3721 tagagtagct tttatgctgt aatgtggttg ggttaacatc tacccatccc tcactatctg
     3781 aaagtgtact ttcttttact acatctaagg gacccatagg tggtgttaca tgtaacatat
     3841 cataacttat tgtttttcta tcgtatgttt tgatatgttc gaatgtagcc acttttttgt
     3901 caccgtcgat ttcaacaagg ttataattat aattgactgt tatatttctt tcttcaacaa
     3961 tcctctctaa ttctttatta tattttccta cgtcaaataa agcatctttt ggcgttgcat
     4021 atatcacatt agcgttagaa cggattttat gtttcctaaa ataatcttca gctaaataca
     4081 taattttcat aggcgcacct ccacacttta taggggtgtt tggatgcgta aaaatggcat
     4141 ttccttgttt aaaattagaa atttggttcc aagtttcgtt aacatagtca ggtgaatagt
     4201 tagagcaaac accattttta cctatatttt cttttagtcc tttaattgaa gaccaattaa
     4261 tctgtaatcc tggagctact actaaaaaat cataataaac gactgtatta tctcctaaaa
     4321 taacgctatt attttcaggt tgaaaacttg aaacagcctg ttttatccag tgagcacctt
     4381 caggtataac actttccata tctttacgag aacttttcaa actagatacc cctgcaccaa
     4441 ccaacgtcca taatggttga tagtaatgat ggtctgctgg gtctataatt gctattttct
     4501 cttttaagtt ttgatttttt cttaatagtc ttgatgctac ggtaacacct gctgtaccgc
     4561 caccaataat aacaatttga taatgcttat tcattttttc cacatcctta aattatttta
     4621 atgattctgg aaaatcttga tagccttctc taatattcac cacattttca aaacctttgc
     4681 tttccaatat acccactgca attgaacttc taacacctga ctgacaatgt acatatattt
     4741 tatcctcttt attaaaagga atattttcat ttaataattt accatgtgga atattaacag
     4801 cttgatataa gtgtccatta ttccactctt catcattacg tacgtctaat acatgttctt
     4861 ctttacctgt catatcagcg ctatgaatgg actgggttga aatgcctgat tttggcaaac
     4921 gatagcctgc taccttatca aacccaatta attgtaaagt gtgtttcgct ttctcaacag
     4981 tagatttatc tccaattaaa tctatatctt tttcaaaatc taagtaccaa ccaatttgat
     5041 taataaagtt tttgttgtat ggtatattga ttgttccttt tgtgtggcca ccgtgaaagg
     5101 cctctttgct acgaagatca aatgctactc tcttattatc taaactagga taaacatcat
     5161 atgattgata taaattcata ccaaactgat taacttgttt catttgtgca aaatgatgcg
     5221 gtggtgctgg ttgatttgat gttaatgatt caataaattt agtctcatca atttcattaa
     5281 atgcccagtt attaattttc tcataaccta ttgtagatat aggtatggca cctaatgctt
     5341 taccacaagg gcttccagca ccatgacccg gccagatttg aacatagtct ggtaaatttt
     5401 taatattttg aacggactca tacatttgtt tcgcgctaat ttctgtagaa ccctttattt
     5461 gaacagattt ttctaataaa tcaggtctac ctatatcacc aacaaaaata aagtcaccac
     5521 taaataatcc catcggaaca cttgaaccac cacctaaatc agtgagtaaa aagctaatac
     5581 tttcaggcgt gtgtcctgga gtatgcagaa cttctaattt aacattgcct acttgaatga
     5641 tatctccatg tttaacaaat tgtgtttttg atggcatatt tttataccct aatgcatctt
     5701 caccttcgcc agacacatat atatttgcat ttaagcgttt agccacatca cgaattcctg
     5761 aagcaaaatc agcatgaata tgtgtttctg tagcttgtgt aattgttaaa ccttcagaat
     5821 ctgcaacttc tatatatttc gataaatcac gaacagggtc tattattatt gcctctcctg
     5881 tacgttgaca acccactaaa tatgatgctt gagataaatg attatcgtaa aactgtttaa
     5941 aaaacatgat aacaactcct tttattaata gatttaatta gataaataaa ttatgattcg
     6001 cattttcagt agcaccaata taagtgccta caccaccgta ctcaacttca tctctaagtt
     6061 cttctttctg aatacccatg acatccatac tcatcgtaca agcgattaat ttgatatttt
     6121 gctcaatagc ttgattgata agtgttggta atgaatcaac atttttcttt ttcattacgt
     6181 agcgcatcat catatttcct aaaccaaaca tattcatttt ggaaagaggc attcgtatat
     6241 tctttttggg caacattaaa tcaaacattt ttgcaatacc ttgcttttta acattaactg
     6301 tttgcacttt ttttaatgca ttaagccccc aaaaagtaaa gaagatagtt acatcttttc
     6361 cagcagctct agcgccattt gcaataatca atgctgctac agctttgtct aattctccac
     6421 taaataatac aattgtagta cctttagcag aataatttat atctaaatct tttgcttttt
     6481 ctttttgaat aatcgcatta attccattgt cattttcatc aagcttaact aaagtatgcc
     6541 ttgtttgttt cacccaactt ttaatatcac tagggaatcc agggtctgtg actttgactt
     6601 caatttggtc acctacttca atattcttca tttcttgact aatttttaca attggccctg
     6661 gacattgaag accacgatag ttaaattgtt tacgatctgc tttaatttct atacttttat
     6721 attcttcttg tgtatcataa ctatcgtttt cttcttcata agctttataa ccaccatcaa
     6781 gatttataac gttataacct tgtttagcta aatatttact agcattagca cttctgttac
     6841 cacttctaca gattacataa taggttttat ttttttcttt attgaatgac tcaatctcat
     6901 caacaggatg aagtatggaa ccattgatat gtcctaatgc atactcctct tctgttctaa
     6961 catcaattaa ttgcccttgc ttggctagtt tttccaattc tgctttacta aattcatcga
     7021 taaacttttc tccatattgt ttcataataa cctccctaaa aatacctgta ggggtatttt
     7081 aaacagttgg atggaacttg tcaaatacct ctaggggtat ttgacacatt tgtgtttatc
     7141 tattaatata tttttattca attaaaagtg gaggtatgac tatgaattat gataaaaaaa
     7201 tgattaatcg tattaataga atacaagggc aactaaatgg aattattaaa atgatggagg
     7261 aaggaaaaga ctgtaaagat gtcattacac aaataagtgc atcaaagagt tcactccaac
     7321 gcttgatgag tatcattatt agtgagaatt taatagaatg tgtaaaagca gctgcggatg
     7381 atgaagaaag ctcccaagag ttaattaatg aagctgtaaa cttattggtg aaaagtaagt
     7441 aatggatatt gttaatatag ttatcatgtt actcattggg gtattcggcg ggttcatatc
     7501 tggtctagta ggcgtaggtg gtgctatcat tatttatcca gctattttat tattaccacc
     7561 attattcgga gcccctgcat atagcgcata tatcgcatca ggtcttactt ctagccaagt
     7621 ctttttcagt acattaagtg gctcattaaa agctcgaaaa aaaacagagt tctcgccaca
     7681 attagttctt tatatgggtg gcggcatgat tatagggagt atgttaggtg catttctagc
     7741 aaatttattt gatgctatat ttgtaaatac ggtatatatc attattgctt tacttgcatt
     7801 aacattaatg tttattaaag tgaaacctag ttccgaaaag tcttcattta ataaatattt
     7861 attagttatc attggccttt tcattggtat catttcaggt atagtaggtg caggtggtgc
     7921 cttcattatt attcctatat tattagtttt attcaaatta ccaatgaata ctgtagtcgc
     7981 taatagtata gtaattgctt ttatatcttc aattggtgct tttgtcatca aacttattca
     8041 aggttatatc cctttatatg acgccttatt ccttataata gggagtatta tatttgctcc
     8101 aatagggtta aaattaagta aaaaagtccc taacataata caaaaatgga ttatcagtat
     8161 tttaatcgtt tttgctatag ttcaactcat tttataaaac taatcctttt aactcagaca
     8221 gcaatttccc gaaaaataat acctcttatt taaacaaata tgtatgcagt tcggatttat
     8281 ataaataaaa atttttgatg aaatatccat atatgcttat aaatataaat ttatcaacta
     8341 taactaaaaa tgataaaaga tttaacgcca ctgatgaccg aattaatatt atttaagttt
     8401 ttatcaaacg catgagtaaa gtagatgcac attgggaatt aatcgaagct attaaaaatt
     8461 tacgtgacga aattgcacca aacacattgt taacaattaa cgatgatatt cccgatagaa
     8521 aaacaggact tgaacttgca gaaaaatatg gtattgatgg catcatgatt ggtagaggca
     8581 ttttccacaa tccattcact tttgaaaaag aaccacgcga acacacaagc aaagaactat
     8641 tagatttgtt gagattgcat ctatcattgt ttaacaaata tgagaaagat gaaatacgac
     8701 aattcaagag tttgcgtaga ttctttaaaa tctatgtgcg tggcataaga ggcgctagcg
     8761 aacttcgcca tcaattgatg aacacacaat caattgcaga agtacgagca ctactagatg
     8821 aatttgaagc ccaaatggac gaagacgtta aaattgaatt atagtatgag taataaggga
     8881 aaggaggaat ttaagtttcc tcctttgttt gtttgtgtga aagtctttta ggtatgaact
     8941 agggtgattt aaatttaata attagtaaac ttgttatcat cagttgtttt taattcgtgt
     9001 tttcttaaat aactagaaaa atgtttaaat aatcttgtat acattaaagt tgaaaagcta
     9061 gtatccatca ccttccctga atgatataag gataaaattt catcattatt caaataatat
     9121 t
//
LOCUS       DAHHYJ010000109         8950 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000109 DAHHYJ010000000
VERSION     DAHHYJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.109"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            79..1602
                     /locus_tag="NBK22_001985"
     CDS             79..1602
                     /locus_tag="NBK22_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415167.1"
                     /translation="MSKKLKIIIPIIVVLLLIGGIAWGVYAFFANTPKNTYLKSEQQT
                     AKMYKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIK
                     MSYGHDPKKEKSMINLEPTIADSALGKFQLAADKDKHYFESPLFKGKYSVNNSDLLST
                     YSKLTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYNKIAEKYSELIVDKLDDDNF
                     DKGKKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKD
                     FEKDIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLE
                     DDKLKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLD
                     NQEKVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEP
                     INFILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1670..2836
                     /locus_tag="NBK22_001986"
     CDS             1670..2836
                     /locus_tag="NBK22_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415168.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALVITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMQSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLATVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2833..3753
                     /locus_tag="NBK22_001987"
     CDS             2833..3753
                     /locus_tag="NBK22_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415169.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHFEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVIHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLIIFM"
     gene            3767..4426
                     /locus_tag="NBK22_001988"
     CDS             3767..4426
                     /locus_tag="NBK22_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2415170.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGQPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWAYTY
                     Y"
     gene            complement(4478..5428)
                     /gene="lytM"
                     /locus_tag="NBK22_001989"
     CDS             complement(4478..5428)
                     /gene="lytM"
                     /locus_tag="NBK22_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCD2415171.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSTQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATAGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNNQAYNSHDGN
                     GKVNSPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMP
                     ENSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGD
                     QIAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPTSYLQSR"
     gene            complement(5754..6746)
                     /locus_tag="NBK22_001990"
     CDS             complement(5754..6746)
                     /locus_tag="NBK22_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCD2415172.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGHTVEVSFKEGE
                     VVIKDNPIGVLTNHPDLNWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNELVALKLTDDLINRKDMTIFKPEKHIIIRKLNDNQ"
     gene            6980..8356
                     /locus_tag="NBK22_001991"
     CDS             6980..8356
                     /locus_tag="NBK22_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2415173.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGDHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSIICLSIFSITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTSLQPSQYIIAVVIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYTPSVAATVTFIVSIILMMLGSLSAYM
                     IIPKTVKS"
     gene            complement(8484..8876)
                     /locus_tag="NBK22_001992"
     CDS             complement(8484..8876)
                     /locus_tag="NBK22_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415174.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKDNYNKYIQGQKQRVIFENGHFV"
BASE COUNT         3001 a         1534 c         1408 g         3007 t
ORIGIN      
        1 acaaaattta aaataaacca tttaataaaa tgtattgttc tgatatgatt aactcataaa
       61 taattgggag gaaacaaaat gtctaaaaaa ctaaaaatta taattcctat tattgttgtc
      121 ttattattaa taggtggaat cgcatgggga gtttatgcat tttttgctaa tacaccgaaa
      181 aatacatacc taaaaagtga acaacaaaca gcaaaaatgt ataaagatta ttttaatgac
      241 cgttttgaaa atgaagtgaa gttccaagaa aagatgaaag ataattcatt tttatcttca
      301 ttagaattaa gcgcagatgc atcagatgaa attgttaagg ggcttggtat tcctaaatct
      361 gttgttaatg cttcgaaaat taaaatgtca tatggacatg atcctaaaaa agagaaatca
      421 atgattaatc ttgaaccaac aatagcagac tctgcattag ggaaattcca gttagctgca
      481 gataaagata agcattattt cgaatcacca ttatttaaag ggaaatatag tgttaataat
      541 tctgatttat tatcaactta ttcaaaactt acaggtgaag atgaagaaac agcaaaagaa
      601 aatggtatta caaaccaaca actaaattta aatactcttt ttagtaatgc tcaagcacaa
      661 caaagtgact acaacaaaat tgccgaaaaa tattccgaac ttattgtcga caaattagat
      721 gacgataatt ttgataaagg taaaaaagaa gaaattaagg ttaatggtga aaagtacaaa
      781 gttagacctg tcacgttaac acttagcaga gctgacacta aaaaaattac attagctgta
      841 ttagaagaag ctaaaaagga taaagacctt aaaaaattaa tggaagaaca aggtgctaca
      901 aaagactttg aaaaagacat caaaaaagca attgacgatg tcaaagaaac taaaaaggat
      961 gaatttgcta aaattcaatc taaaatttat accgaaaaac atacgattgt aaaacgagaa
     1021 attactatta cagacaaaga aaataataaa actaaaatca aaggtactaa tactttagaa
     1081 gacgataagt taaaactaga ttacgcactt gatttcgatc aagataaata cacgtatgct
     1141 gaagcgaaat atacaattaa aggcgtatct tctaaggaaa aagacaataa atacagtgat
     1201 aaatacgaat ttggtaaaaa gacagaatat gatgaatcaa aaatcaaatt agataaccaa
     1261 gagaaagtag atggcacaaa acgtcaagat aaaggtaaaa ttactgtcgc attagataaa
     1321 tatagcgacg aaaatgaatt cacttttgaa aataatatag attctgacgt aaaaaataac
     1381 actcagaaat ctacgttaaa tatcggcatc aaatatgctg aagaaccaat taatttcatt
     1441 ttaaaatcta gcacaaaatt gaaagcagat attgattttg atgatagtgg tgcgaaagat
     1501 ttcaatagtc tatcttcaaa agaccgtgaa aaacttgaaa aagaaatcga aaaaaatggc
     1561 ggcaaaatgt ttgaatcaat tttaaaaaag gcatctaaat aactaaaata taaaaataag
     1621 attggaaggt catcttgcct ttcaatctta tcattggagg agtggttaaa tgaaaacaat
     1681 acatttgttt cgcatctacc actctttttt attgaaaaag tggtatttga ttatctattt
     1741 actatttata ttagcggcac tcgttattac attaacgacg atccaacatg taacagaaga
     1801 tgacaatcat tttaatatag gtgtcgtaga taaagatcaa tcaagtgaaa cgaaattaat
     1861 cttaaactct attggtaaag ggagtaacct aggaaaaaac gtgagcatta aagcatatga
     1921 tgataagcaa gcacatactt tgttaaaaaa acataaactt caaggctatt ttgtttttga
     1981 taaaggtatg accaaggcat tttataaaca aggcgaacta ccaatttcag tatatacata
     2041 tgaccaacaa tccatgaaaa gtgtcgtgct atctcagcta acagattctg tttaccaacg
     2101 tcttatgcaa tcaatgggtg gcatcttagc ttttcaagac ttagcaccga aagcatcaca
     2161 ttctgacagt atcaatgtta tgactgattt gctgattaca ggattaaacc gttcaggtgc
     2221 atttaactta gaaccgattc atttatacga tacgggcagt tattatgcaa ttaccggatt
     2281 tttagcaacg gtattcatct ttgcactatc tttatttaca gttttgaaaa tgaatcaaga
     2341 tactgtattg aaagcgcgat tgaaaatgtt tcatttttct aaagagcgtt tgttaatcat
     2401 tcgtgcgttg attacatggt tttatactat gttatggagt atcgttggtg tagtttggat
     2461 tgtgttcagt attccgaata cctttgaatt atataattgg ccaacgttag ccattcattt
     2521 aagctattat gttacctttt taatactgtg gttattactc atcgaacttt taacaacagg
     2581 cttgttgaat agtattagta aagtcatatt agctattgtc atcctggtgt tatccggttt
     2641 aacaatacct acgatttttt tacaacatat agcaaatggc gttttcaata ttcaaccgtt
     2701 cgcagttgta acaaatcaat tgttagaaat tattttaaac aactacattt tagaactaca
     2761 ccctagtttc tatcttagct tcatcgcact tttgataatt aatttagctg tattggtttg
     2821 gaggtatcgt caatgaagcc ttatatacaa cttgttgtgt tcaagcaatg gttacaatac
     2881 atcttgctcg taacaaccat tgtcatcgca ctcgtactta ttggtatcgg ttaccgtgta
     2941 gcacatgaca acttcaaaat accgattacc attcaagatt tagaccaaac cactgcatca
     3001 aaatcattcg tcaataaaat taaacaatct gactatgtaa ctattaaaaa agtcgatgaa
     3061 gatgaaagct atattgaaga tgatgttact aaaaaggaag ctattttaag tatgcaaatt
     3121 cctaaaggtt tctctcaaaa attaaaagag aaccgtttaa aagaaacgat acagttatat
     3181 ggtagagatg actttatagg tggtattgct gtagaaattg ttagtagttc attatatgaa
     3241 cagcaaattc ctaacattat ttatgaacac tttgaggata tgaaacagca tcaatccatc
     3301 gatgctatca acaagtccta tcataaacat acacctgaat ctaaaatcaa atttgtgtcg
     3361 cttactaaac aagcacaaca ctctatttca attagcttaa tctttgcggt gattttattt
     3421 gttagcgctg ttcaagtagt aattcattat cgtttaaacc aacaagcagc attgcaacga
     3481 ttatcacaat atcatttaag ccgtttcaaa ctatatagta cttatgtaat gacacatacg
     3541 attttgttat tgttggtact attggcagtt agtctatatt tgtctcaacc actcagctta
     3601 atattttact taaaatcact gttacttata ttgatttatg agataggtat cgtctttatc
     3661 ttattccata ttcaaacaat aagtcatcga ttattcatga catttatata tgcacttgct
     3721 atgggtatcg tatacttgat tattttcatg taaaggagcg taactgatga tagaaattaa
     3781 taacctttca aagcgttacc gtaacaagca gattttcaat catttaacta tgtcctttga
     3841 tagtaatcgt ttaaccgtat tacttggtga taatggtgct ggaaaatcaa cattacttcg
     3901 tatgattgct ggtattgaaa aagctaatga tggaactatc aactatttcg gcgaaaaatg
     3961 gaatcaaaga caaatacaaa atcacatcgg ttatgtgcca caagacattg cgttatttga
     4021 acacatgaca gtggctgaaa acattaaatt ttttaaatca ctttgtaaaa atccaattaa
     4081 cgatacaact atcaacgaat atttacagca attaaatttt gatgatacgt ctgccaaagt
     4141 atctacattg tccggtggga ataaacgtaa aattaatata ttagtaggtt tactaggtca
     4201 acctcgaatt ctcattttag atgaaccgac agttggtatt gatttaaaat ctagacatga
     4261 catccaccaa ctacttaaca tcatgaaatc taaatgttta attatattaa ctacccatca
     4321 tttagatgaa gttgaagcac ttgcagatga tatcaagtta attggccaag atccttttta
     4381 tcaacatgtc ttagaggaca agcaatgggc ttatacctat tattaaaacg aaaaaatccc
     4441 aagttgcgta tgatatcgta acttgggatt tactgtatta tctactttgc aagtatgacg
     4501 ttgggtctac tgcatattga ttaccgatgc caccagacat acgttggaag tgtacgtgag
     4561 gtgctgttga attacccgta ctacctgaat atgcaatttg gtcaccagct ttgactttat
     4621 caccagctga aacagttaaa cgattattat gcatatacca ttggtagtta ttactgttcg
     4681 cttctttaat cgttacttga ttaccaccac catagttact ccaacctgct tgtactactg
     4741 taccatcagt taatgagtaa actggtgaat tttcaggcat tgcatagtcg acaccgtaat
     4801 gcgcaccacc accgtgatat tgtccatatg gttgtagttg tttacgactt gttaaccagc
     4861 ttgcgtcttt cgcatgacca ctagctgtcg ctttacttgc tgatccgcca ttttgattag
     4921 acgtgccatt aggggaattg acctttccat taccatcatg gctgttatac gcttggttgt
     4981 tgttactatg tgaataatag ctcgcgtctg gacctacatt tgattgatag ccatattgat
     5041 taatatgctg ttggctttga ctcgctgtgt agtcattgtt atctcctgct gttgctggat
     5101 tcacatatgt ttggccgctt ccattggcat ttgcattttt tggataacag ttataaaaat
     5161 aatgcgtatg tccttgagca tctacgaatg tatagctata ttctttatta tcaaacattg
     5221 cttgattcca gttaccatca ggtgtgtgat gataatcccc attagaatca attgtataat
     5281 aagtaccata agatacgtct tgtgattgtg ttgacatttg tgtatgtgct tgttgggtgt
     5341 ttgtcgtttc tgctgcatct gcttgatgcg ccattgtaaa tgtagcgaag cccatcgttg
     5401 caatcgctgc tgctgttaat tttttcatgt ataaaacatc ctccattaaa gttaaagtta
     5461 gttttcaatt aaactgtact gcacatacta aaagaattag acaactgagt aaaggattta
     5521 attctcattt tccaactatt taatattccc gaaatgtttt actaaactca ttacattgtc
     5581 attacaaaat agccatacat tgatattaaa atgacatctc tcactgcatt cgtttaacct
     5641 tttataaatt ttcaaaacta acaactaatc attcgtccat gttcgcgctt caacgctaat
     5701 gtatgatagt aatcatccat caaatcatat caaccaaatt ccattatcaa tcgctattga
     5761 ttgtcattca actttctaat aatgatatgc ttctcaggct taaaaatcgt catatctttt
     5821 ctattaatta aatcatctgt gagctttaat gctactaatt cattgctgcc ataatactta
     5881 atatataacg ttcttgtagt taaatttatt acggtctgat acatcgtata gtgattgtca
     5941 gcatcatgcg gacgtacaat tccaatcggt atatttaccg catctaataa ataaaatgca
     6001 ttcattaaat ccatttcttt atcattgttt tgagcaatgt ttgctttcat aaatgccatt
     6061 ctcacaaagc gctcagttga agtaaatcca cctggcaatc caaatgtacc tgcttcattg
     6121 cctaaaggtt caatcgttac accttccaat aaatttgctg ttgctggata aggagaaata
     6181 ttgatatatt gtcttaaatt actataatgc caatttaagt ctggatgatt tgttaagaca
     6241 ccaataggat tatcttttat aaccacttca ccctctttaa atgaaacttc gactgtatgt
     6301 ccagttgcat cggaaacatg ataatgcaat ggcggaactt caccgatgtc atttaaatat
     6361 acagctacaa catgtatttg ggatgcttgt tgtttcatat cttcaatgct tgttgtatat
     6421 cccaaaatcc atgtcacaat ttcattttgc gtaatattca tcgcgtccgc tttgtgtgtt
     6481 gatccatatg aactataacc tcggaaatat tgtgttgaaa tggcaacgcc atgttcatta
     6541 acaccatcac cataaataaa accttccata tctgttcctg tgccaataaa gccatattgc
     6601 gtttggcctg tcgtgccagt gcgagatgtc caacgataat ttctaggcgt cactgctggc
     6661 gaaccatcta atggataatc ataatccatc gtgcgtccaa gaagtacttg attatttaaa
     6721 gtttgtattg tgaatcctgt gcacattgtt ctcactcctc tgtaccttca tttactttaa
     6781 tcactttcaa ataaagctgt ttcacttaaa catactataa aaaatcaatt atacaagcaa
     6841 ttaattgata ttcattctca ataactgtgg tatgatatgt aaggaaatca tgacttatgt
     6901 gtgagtgaac gatcatctat acatccgttc acttcatctc atgactttct atatttaatt
     6961 tttacaagga gtgacatctg tgaataacac acaatcttca ccacgcagta atattattat
     7021 tgcgattatg ttgtctgcat taacatattg gttgtttgca caatcattta ttaatatagg
     7081 acctctcgtt ggtcaaacgt atcaaacctc tcctgccgtg ttaaatttat ctattagttt
     7141 aacttccttc gccacaggta tcttcatggt ggctgcaggt gatattgctg ataaaatagg
     7201 acaactgaga atgacataca tgggtctcat aatcagtatg tttgcatctc ttctattaat
     7261 tatatcggac atcactgcac tgctcatcat cggtagaatt ttacaaggtc tatcagcagc
     7321 tatcttgtta ccttcaacag ttggcgtttt aaataatcaa tttaaaggag atcatttaag
     7381 acgagcgatt agttatctaa tgattagtac tgttggtggc atcggcctag ctggtgttat
     7441 cggcggttta attgccacaa atttcggatg gcaaacgaat ttcatcatta gtatagtcat
     7501 tgctttcatt gccatattgc ttctaaaagg cacacctgaa aaagtaagtc aacatagcca
     7561 ccgtcatcca ttcgattaca aaggtatgtc gattttcgct gttatgattg gtagctttac
     7621 attattgtta acacaaggat tcgaacaagg ttggtttagt acattttcaa tcatttgtct
     7681 gagcattttt agcatcacta cgttgatatt catcatcatc gaacgtcgac atgaagtacc
     7741 ttttattgat ttctcagtat tacgcaaccg tccgttcatt ggtgcatttt taaataactt
     7801 tgttttaaat agtggtctag gcgtaacagt ggtctttttc atatatgctc aaacacacct
     7861 tggtttatca gctgcgcaat ctggacttgt tacattgcca tatgccattg tggcagttgc
     7921 gatgattcgt ttaggtgaaa aagcaacatt acgtttcggt ggcaaattga tgctcatcat
     7981 tggtccgttg ttccctgtca tcggcatcac tattattagc atgacatcac ttcaaccatc
     8041 acaatatatc attgctgttg ttattgggtt tgtcatttgc gcaattggaa atggtttagt
     8101 cgcaacaccc ggccttacaa ttgcaatttt cagtatgcct aatgaaaaag ttggtttagc
     8161 tacaggatta tataaaatga gtggcacact aggtggcgcc tttggtatag cgttaagtac
     8221 aactgttttc agtatgttac aactaaacta tacgcctagt gtagctgcaa ctgtaacatt
     8281 tatcgtcagc attatattaa tgatgctcgg ctcattgtct gcatacatga tcattccaaa
     8341 aacggttaaa tcttaaatat aatagaagca tcatgtttcg aaatatcttt atcactttaa
     8401 aatgatatac aagaaattca agaaaaataa gcgaagaacg tttttctctg ttcttcgctt
     8461 actctaatta tattcactat caattagaca aaatggccat tttcaaatat cacgcgttgt
     8521 ttctgacctt gaatatattt attataatta tccttttgaa aatcagttaa cattaattta
     8581 gatgtaccgt attttaacac tttttgcatt gtttctattc tcatttttct aaataaccat
     8641 ccatctttta acacaatacg attaacagca tcatatgata attctactgt ttctttaatt
     8701 tcaaatgtct tgaatgaaat aatcgtgcac attaaaaacg tatcaccaaa gtaataaaca
     8761 tctaaatcat cacgtttatg ttgtccaact aacaaacgac catattcgaa ctctttttct
     8821 ggatatttca attctaaaaa actaataatc tcttcttctt ttaatttgaa ttgcatttaa
     8881 aaacatcctc tcttaagttt taacaagcct taattaaaaa ttttttcaat cacatagttc
     8941 aatatacatc
//
LOCUS       DAHHYJ010000110         8942 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000110 DAHHYJ010000000
VERSION     DAHHYJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.110"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            327..716
                     /locus_tag="NBK22_001993"
     CDS             327..716
                     /locus_tag="NBK22_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCD2415175.1"
                     /translation="MLHLHILSWVLAIILFIATYLNISKNQGGTPYFKPLHMILRLFM
                     LLTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWI
                     TIALIIITMVLGVILPLGPISKLFGIG"
     gene            complement(887..2203)
                     /locus_tag="NBK22_001994"
     CDS             complement(887..2203)
                     /locus_tag="NBK22_001994"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCD2415176.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDADMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(2255..3079)
                     /locus_tag="NBK22_001995"
     CDS             complement(2255..3079)
                     /locus_tag="NBK22_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2415177.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            3193..3501
                     /locus_tag="NBK22_001996"
     CDS             3193..3501
                     /locus_tag="NBK22_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCD2415178.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            4049..5863
                     /locus_tag="NBK22_001997"
     CDS             4049..5863
                     /locus_tag="NBK22_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD2415179.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLMG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIVLILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     YVYFIDISLTIIFAELSYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFML
                     ILVGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNN
                     IKPLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVL
                     ELGTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLID
                     WNKRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
     gene            6066..8675
                     /gene="clpB"
                     /locus_tag="NBK22_001998"
     CDS             6066..8675
                     /gene="clpB"
                     /locus_tag="NBK22_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCD2415180.1"
                     /translation="MDINKMTYAVQSALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(8734..>8942)
                     /locus_tag="NBK22_001999"
     CDS             complement(8734..>8942)
                     /locus_tag="NBK22_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2415181.1"
                     /translation="SIKLIEMNQGISFLPLYITTNSDYNISVINTKILQAPISFTYIY
                     SKKESPEILVFIKSFKKYIANEQL"
BASE COUNT         3100 a         1253 c         1600 g         2989 t
ORIGIN      
        1 cagctttttt aaattgccta agaacttaat gtcccagcct ggttttttct tgcttttcta
       61 aaaaggtgtt aaagataatt gtttataatg ttaccatttt gagatgaaag tgaaatattg
      121 atattaagaa gtagttgatt attttacagc agatgcacaa tattctaata aggaataaaa
      181 tgtcatgttc ttcctctcaa atatagaagt gtggtagaat atatattcgt gtataatcaa
      241 atcatgatta aattacaagc aagtgggtat taatcccaag aagctttaat ttaataatat
      301 aaattattaa agtgggggga atcagtatgt tacatttaca tatattaagt tgggtattag
      361 cgattatttt atttatcgct acatacttaa acatttcaaa aaatcaaggt ggtacgccat
      421 atttcaaacc gttgcacatg attttacgct tatttatgct gttgacgtta atttcaggat
      481 tttggatatt aattcagtca tttatgaatg gcggggcaaa tcatatgttg cttacattga
      541 aaatgctgtg tggtgttgca gtagttggat tgatggaagt gtcgattgct aaaagaaaga
      601 gacatgaaca aagtcacaca atgttttgga taacaattgc attaattatc atcacaatgg
      661 tattaggtgt cattctaccg ttagggccta tatcaaaatt attcggtatt ggctaattga
      721 taagacatat agtatatgac ataaggcatg gacaattatt agtccaagac cttatgtctt
      781 ttttgattct aaaaatgtta aacataaaaa atggactgga tagtagtacc agtccaacat
      841 aattctaacc tagacaatta agattagatt gaatcgcatg attcatttat ttagctttgt
      901 aaccaatcat attgattaaa tctttagggt ggctatatgg tggtgcataa gccacttcaa
      961 actcagttaa ctcatctaca gttagctggt tcatcattgc catcgatagt acatcaatac
     1021 gtttatctgc accttctttt cctactgcag ctgctcttaa aatctgacgg tttgaagtgt
     1081 cataatatac ccttaagtgt aaaggggaat ttcctgggta ataattcgcg tgtgcacctt
     1141 gagtgacttc caccatttta tagtcaaatt gctttagttc atttggttta acgccgacac
     1201 tcgcaaatgt ataatcaaag aacttcacaa tattgttgcc taagaagcct ttgaattcaa
     1261 tagtgtcatt tccagcaatt tgttcggcaa caatacttgc tgcacggtga gcgccccaag
     1321 ctaaaggaac actagccggt agatcgacat gtcgataatg tgatgttgca atatcgccta
     1381 ttgcataaat gtttggaaca tttgtttcaa atttatcgtt taccggtatg aaaccttttc
     1441 gatcaagttt gatatttgaa ctttcgataa attttgaatt ggggtgagta ccgacacctt
     1501 caataatcat atcgtaatgt tcaacttttc ctgatttaaa tgtaatttca tttccattga
     1561 tagcatcaat ttcctcattt aaacggtatg gaatctcccg cttatctaat tcatcaagta
     1621 taggttgatt catgtcagca tccattaatt tatttatctt atcagatcga tgaattaaag
     1681 tagggtgtaa accacgttcg taaagatttt caagaacttc taatgaaaca taccctgcac
     1741 ctacaactaa tactttatca acttgatttg ctttgatgaa ttgatcgata gcatcagtgt
     1801 cttctaaatt tctaagtgta aatgtaatat cactttcaaa gccaaggcta tttgcacttg
     1861 caccagggct taaaatgagt ttatcgtaag attcttcaaa ttgttcgttg gtctttctat
     1921 ttaatacagt tacagtttgt ctttcatcat tgattgcaat aacttcatga taagttttta
     1981 ctgtaatttg ctttctatca taaaattttt caggtgtata cgctaaagca tattttctat
     2041 cttcaacaac ttcgccaatg acataaggca atgcacaatt agcaaagctc atatcacgat
     2101 ctttttcaaa aataataatg tcactttctt tatctaaacg tcgaatttgg ctggcacatg
     2161 ttgcaccgcc agcgactgct ccgactacga ctattttggg catcgtaact tacctcctat
     2221 tgttttaaaa tattataaaa tgattagtta taaattaaca gtaatatcta atatttaaat
     2281 taaagaaatc attcaaatat cgaccaatgc catcttcatt attgttgaat gtaatattgt
     2341 tcgctacatc tttaagttct tgcaaaccat tttccatagc aacaccatgg cgggcgtact
     2401 caatcatttc aatatcatta tcttcatcac cgaatgcaat aatattattt cggtcaatat
     2461 ttaaaaattg tctaacttgc tcaatgcctc ttgctttatt aataccaagt tttacaattt
     2521 caatgacagg gaatggtgcg ccccagcgtc gatgctcaat atgatcggca taaaaatgag
     2581 taagcatatt tttgatttca ggtattttac tttcttcggc ttcaattaaa attgaggtag
     2641 gggattcttt caagtggaca agtaaattac cagtttgaat tcttggatta cccattgaaa
     2701 aaccttcaaa taatcttgga tcatgattgt taatgaaaac ataatctttc acttctgcta
     2761 taatattcga tacttgatat tgttgtaatc cttgaataat gttttgtgcg atgcctaaat
     2821 ctaaaatttc atggcaagtt ttgaagtttt tatctttagg gtgatgtacg taagcgccat
     2881 taaaattaac aattggtgtc gttaaattta attcatgata atacatttga cttgcacgat
     2941 aaggtctgcc agtcgcaatc ataatttggt gtccacgttg ttgtaattca tttaatactt
     3001 gtttagtata tgatgaaatt tctttgttat cgtttaataa tgttccgtct aagtctagac
     3061 atattaaatg tggttgcata tctctctcct cataagttgc ttagttcata tagatacatc
     3121 atagcatttt attggcgttt tgtcggatat ttgatatatt atgaataata tgacattaaa
     3181 gaggtgatat ggatggaaga ggcattgaaa gatagtatct taggtgcatt agaaatggta
     3241 attgaccctg aattaggaat tgatatcgtt aatttaggtt tagtatacaa agtgaatgtt
     3301 gatgatgaag gcgtatgtac agttgatatg actttaacat caatgggatg tccaatggga
     3361 cctcaaatta ttgatcaagt taaaacagta ttagcagaga ttcctgaaat tcaggatact
     3421 gaagtgaata tcgtatggag tccaccttgg acaaaagata tgatgtcacg ttacgctaag
     3481 attgcacttg gtgtgagcta acataaacgt gcattgcctg tagaatttct tttagaaatt
     3541 ctctgtgttg ggtccccgca gtcaatataa cattgtagag tataattcat cgtgaataag
     3601 ctcccttcaa agtagacatt gaaaaatgaa aattttgaag ggagccttat ttatgttcta
     3661 ggttctgatt tcattttgaa aatttatatc taacttgttt caaaaaatat gaattaagac
     3721 agtgacttgc acttccaaga gtgcacaatt cattttctag ttgaatattt tcttgaaaat
     3781 ttttataata aattgaattt atattacagt tatattacaa tacataatac ttcatagttt
     3841 ttaagttgcc gagttgcatt acaaattgga tttcgaagta agaatcatca ttggtaattt
     3901 ttttaattta acagtatttt agtcaatatg attttgatga tgtcgtaatt caataatata
     3961 catatgcatt tcaatattct tttaaacagc ataactaaat ttaattaagc atctaagaag
     4021 aacagtttta aaagggaaga atgcaaaaat gaacaaaaca aagggtttta caaagtataa
     4081 gaaaatgaga tatatgccag ggctcgatgg tttgagggca atcgctgttc taggaattat
     4141 tatttaccac ttaaacaagc aatggttgac aggtggcttt ttaggtgtgg atacattttt
     4201 tgtgatctct ggttatttaa ttacaagctt attactcaaa gagtatgatg acacaggtat
     4261 cattaaattg aaaagctttt ggatacgtcg tttaaaacgt ttattaccag cagtaatagt
     4321 tttattaatg gttgtaggga cagcaacctt attattaaaa tcagataata tcattagggt
     4381 taaacatgat attattgctg cgatatttta tgtatcaaac tggtggtata tagcaaaaga
     4441 tgttaattat tttgagcaat tttcatttat gccattaaag catttatggt ctttagcaat
     4501 tgaagaacag ttttacatat ttttcccagt tattttggtt acattattgt taacaattaa
     4561 aaagcgatac aaaataggat ttattttttg gggagtatca ataatttctt tagggttaat
     4621 gatgtttatc tacagtatta atggggatca ttcacgagtg tattttggta cagatactag
     4681 attacagaca ttgttaatgg gtgttatttt agctttttta tggccaccgt ttaaattgaa
     4741 aaatgatcca cctaaagttg taaaatatgt tattgatagc ataggtagtt tatcatttat
     4801 agtacttata ttattatttt tcattattaa tgatgagacg aattggatat atgatggtgg
     4861 tttctattta atatccatat taacgttatt tattattgcc agtgtcgttc atccatctac
     4921 atggatagcg aagatatttt caaatccagt gttagtattt atcgggaaaa ggtcttatag
     4981 tttatattta tggcattttg cagtaattag tttcgtacat agttactatg tagatggaca
     5041 gatacctgta tatgtgtact ttatagatat aagtttaaca attatatttg cagagctatc
     5101 atatcgcttt atagaaactc catttagaaa agaaggtatt aaagctttaa attggcgacc
     5161 ttcttatata ccacaattta taagaatggc aattgtagta accttgttaa ttccatttat
     5221 gttgatttta gtaggtgcat tcaataaata tggtaaagac attattggag aaaaagcgaa
     5281 tagctttgat accactattg aagataatta tttaatgcgg atagcaccaa ttgataacat
     5341 tcacattgat ggcttagtaa gtgagaagaa aaaggaatct tccgacgtat ataataatat
     5401 taaacctctt ttaatcggtg attcagtaat ggttgatatc ggtgagtcat ttaagtcatc
     5461 agttcctaag tctagaattg atggaaaagt agggcgtcaa ttgtatcaaa ccttaccttt
     5521 agttaaagcg aattattcac aatataaaaa atcatctgat caagtcgtat tagaattagg
     5581 tacaaatggc gactttactg tcaaacagct cgacgattta cttaatcaat ttggaaaagc
     5641 caagatttat ttagttaata cacgtgttcc aagaatttat gaggcaaatg taaatcgatt
     5701 attagctgac gcggcgaaac gaaagtccaa tgtcacatta attgattgga ataagcgatc
     5761 acaaggacat agtgaatatt ttgcaccaga cggtgtacat ttagagtaca aaggagtcct
     5821 agctttaaaa gatgaaatat taaaagcact taaaaagaaa taacatatgt ttaataagtc
     5881 tagttaatgt gtaacgtaac attagctaga tttttttatt caaaaaaata tttacaaata
     5941 ttaggaaatt taagtgtaaa agagttgata aatgattata ttgggactat aatataatca
     6001 aggtcaaaga aagtcaaagt caaaagtgca acttgaccaa ctctacaaat tagaggtgaa
     6061 attatatgga tataaataaa atgacatatg ctgttcaaag tgctttacaa caagcagttg
     6121 aactgagtca gcaacataaa ttacaaaata tagaaattga ggcaatttta agcgctgcct
     6181 taaatgaaag tgaaagctta tataaaagta ttttagaacg agcaaatatt gaggtagatc
     6241 aattaaacaa agcttatgaa gacaaactaa acacgtatgc atctgtagaa ggtgacaata
     6301 tacaatatgg tcaatatatt agccaacaag caaaccaatt gataactaag gctgaatcat
     6361 acatgaaaga atatgaagat gaatatattt caatggagca tattttacgt tcggcaatgg
     6421 acattgatca aacaacaaaa cattatataa ataataaagt agaagttatc aaagaaatta
     6481 ttaaaaaagt aagaggggga aatcacgtga catcacaaaa tccagaagtt aattacgaag
     6541 cattagctaa atatggccgc gacttagtag aagaagttag acaaggtaaa atggatcctg
     6601 ttataggaag agatgaagaa attcgaaata cgattcgtat tttaagtcgt aaaactaaaa
     6661 acaaccctgt gctcattggt gaaccaggtg ttggtaaaac tgcaattgtt gaaggattag
     6721 cgcaacgtat agttaagaaa gatgtgccag aatcattatt agataaaact gtttttgagt
     6781 tagatttaag cgcattagta gcgggcgcta aatatcgtgg tgaatttgaa gagagattaa
     6841 aagcagtcct aaaagaagtt aaagagtctg atggtagaat tatattattt attgatgaaa
     6901 tccatatgct tgtaggtgct ggtaaaacag atggtgccat ggatgcaggc aacatgctaa
     6961 aaccaatgtt agcacgagga gagttacatt gtattggtgc aacaacttta aatgaatatc
     7021 gagaatatat tgaaaaagat tcggcattag agcgtcgttt ccaaaaagta gcagttagtg
     7081 agcctgatgt tgaagataca atttcaattt tacgtggttt aaaagaacga tatgaagtgt
     7141 atcatggtgt gcgtattcaa gatagagcct tagttgctgc cgctgaattg tctgatcgtt
     7201 acatcactga tcgtttttta ccagataaag cgattgattt agttgaccaa gcatgtgcaa
     7261 caattcgtac ggaaatggga tcaaatccaa ctgaattgga tcaagttaat agacgtgtca
     7321 tgcaattaga aattgaagaa agcgcactta aaaatgaatc tgacaatgcg agcaaacaga
     7381 gattacaaga actacaagaa gagcttgcca atgaaaaaga gaaacaagca gcacttcaat
     7441 ctcgtgtaga atcagaaaaa gaaaaaatag caaatttaca ggaaaaacgt gcgcaactag
     7501 atgaaagtag acaagcgttg gaagatgcac aaacaaataa caatttagaa aaagcagctg
     7561 aactacaata tggaacaatt cctcaattgg aaaaagaact tagagaatta gaggataatt
     7621 tccaagatga gcaaggtgaa gatacagatc gaatgattcg tgaagttgta acagacgaag
     7681 aaattggcga tattgtcagc caatggacag gcataccagt ttcaaaatta gttgaaacag
     7741 aacgtgaaaa attacttcac ttaagtgata tcttgcataa acgtgttgta ggtcaagata
     7801 aagcggttga ccttgtttca gatgcagtag ttagagctag agcaggtatt aaagatccaa
     7861 acagacctat tggtagtttc ttattcctag gtcctactgg ggtaggtaaa actgaattag
     7921 ctaaatcatt agctgcatca ttatttgatt ctgaaaaaca tatgattcgt attgatatga
     7981 gtgaatatat ggaaaaacat gcagtatcaa gattgatagg ggcacctcca ggatatattg
     8041 gacatgatga agggggtcaa ttaactgaag cggttcgtcg taatccatac tcagttatct
     8101 tattagatga ggttgaaaaa gcgcatactg acgtctttaa tgtattattg caaattttag
     8161 atgaaggccg tttaactgat tctaaaggac gtagcgttga ttttaaaaat actattatta
     8221 ttatgacaag taatattgga tctcaagttt tattagaaaa cgtaaaagag actggtgaaa
     8281 ttacagaatc aacagaaaaa gctgttatga caagtttaaa tgcatatttc aaaccagaaa
     8341 ttttgaatcg tatggatgat atcgtattat ttaaaccatt atctattgat gacatgagta
     8401 tgattgtaga taaaatctta acgcaattaa atataagatt attagaacaa cgaatctcaa
     8461 ttgaagtttc tgatgatgct aaagcttggc taggtcaaga agcttatgaa cctcaatacg
     8521 gtgcaagacc attaaaacgt tttgtacaac gccaaattga aacaccatta gcacgtatga
     8581 tgattaaaga gggattccca gaaggtacaa cgattaaagt taatttaaat tcagacaata
     8641 acttaacgtt taatgttgaa aaaattcatg aataaaattg aaccaagaat gtgatgatta
     8701 atcgcattct tggtttttta gatttgaaat ttattataat tgttcattgg caatatactt
     8761 tttaaatgat ttaataaaca ccaatatttc tgggctttct tttttgctgt atatatatgt
     8821 aaatgaaata ggtgcttgta aaattttcgt gttaataacc gaaatattgt aatcactatt
     8881 tgttgttata taaagaggca ggaatgatat accttgattc atttcgatta atttaattga
     8941 ag
//
LOCUS       DAHHYJ010000111         8810 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000111 DAHHYJ010000000
VERSION     DAHHYJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.111"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            262..1791
                     /gene="aur"
                     /locus_tag="NBK22_002000"
     CDS             262..1791
                     /gene="aur"
                     /locus_tag="NBK22_002000"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCD2415182.1"
                     /translation="MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVT
                     QKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKGDAADKAFKAVKIDKNKAKNLKDKVIKE
                     NKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGV
                     LGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            2158..2607
                     /locus_tag="NBK22_002001"
     CDS             2158..2607
                     /locus_tag="NBK22_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCD2415183.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2610..2777
                     /locus_tag="NBK22_002002"
     CDS             2610..2777
                     /locus_tag="NBK22_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415184.1"
                     /translation="MYQMNNKNLFRITCTQVSYQILTCVRVFLWKERIHSHSIDCIKN
                     FIQHVFMGIFE"
     gene            2954..4189
                     /gene="arcA"
                     /locus_tag="NBK22_002003"
     CDS             2954..4189
                     /gene="arcA"
                     /locus_tag="NBK22_002003"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCD2415185.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4222..5232
                     /gene="argF"
                     /locus_tag="NBK22_002004"
     CDS             4222..5232
                     /gene="argF"
                     /locus_tag="NBK22_002004"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD2415186.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKYGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5323..6753
                     /gene="arcD"
                     /locus_tag="NBK22_002005"
     CDS             5323..6753
                     /gene="arcD"
                     /locus_tag="NBK22_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCD2415187.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLPSIIVASLLLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFSMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYTIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6770..7711
                     /gene="arcC"
                     /locus_tag="NBK22_002006"
     CDS             6770..7711
                     /gene="arcC"
                     /locus_tag="NBK22_002006"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD2415188.1"
                     /translation="MKEKIVIALGGNAIQTTEATAEAQQTAIRCAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFDNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFVEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7810..8514
                     /locus_tag="NBK22_002007"
     CDS             7810..8514
                     /locus_tag="NBK22_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD2415189.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
BASE COUNT         3252 a         1197 c         1677 g         2684 t
ORIGIN      
        1 gattaagtat atagagcact attttgtatt tgttaatatt ttcacaaaaa taaagccttg
       61 ataaatttta aaatataatt aagctcaatt tttaaaattt tatttagcta cagataacat
      121 ttttaaaaaa gaaaagaatc aataaataaa atcaacgaac aaaaagtata gaaataaata
      181 gaaataatca tttacttttc tgaaaaatta aattaatatt ttatttataa gctgttttta
      241 agatttcagg aggaatgaaa tgtgaggaaa ttttcaagat atgcatttac aagtatggca
      301 gcattaacct tgttgagcac tttatcacca gcagcattag cgattgattc aaaaaataaa
      361 ccagctaatt ctgatattaa atttgaggtg actcaaaaga gtgatgcggt caaagcatta
      421 aaagaattgc ctaaatccga aaatgtaaaa aatatttatc aagattacgc tgttactgat
      481 gtaaaaactg ataaaaaagg atttacgcat tatacattgc aaccgagtgt tgatggtgtt
      541 catgcacctg acaaagaagt gaaagtacac gcagacaaat caggaaaagt cgttttaatc
      601 aatggggata ctgatgcgaa gaaagtaaag ccaacgaata aagtgacatt aagtaaaggt
      661 gacgcagccg acaaagcatt taaagcagtt aagattgata agaataaagc gaaaaatctt
      721 aaagataaag tcattaaaga aaacaaagtt gaaatcgatg gtgacagtaa taaatacgtt
      781 tataatgttg agttaattac agtgacacca gaaatttcac attggaaagt taaaattgat
      841 gctcaaactg gcgaaatttt agaaaaaatg aacttagtta aagaagctgc agaaactggt
      901 aaaggaaaag gtgtacttgg cgatacaaaa gatatcaata tcaatagtat tgacggtgga
      961 tttagcctag aagatttaac gcatcaaggt aaattatcag catttagctt taatgatcaa
     1021 acaggtcaag caacattgat tactaatgaa gatgaaaact tcgtaaaaga tgagcaacgt
     1081 gctggcgtag atgcaaatta ttacgctaaa caaacatatg attattacaa agacacattt
     1141 ggtcgtgaat catatgacaa ccaaggtagt ccaattgttt cattaacgca tgttaataac
     1201 tacggtggtc aagataacag aaataatgcc gcatggatcg gtgacaaaat gatctatggt
     1261 gatggtgatg gtcgcacatt cacaagttta tcgggtgcaa atgacgtagt agcacacgaa
     1321 ttaacacacg gtgtgacaca agagacagcg aacttagaat ataaggacca gtcaggcgct
     1381 ctaaatgaaa gcttttcaga tgtttttgga tactttgtag atgacgagga tttcttaatg
     1441 ggtgaagatg tctacacacc tggaaaagag ggagacgctt tacgcagcat gtcaaaccca
     1501 gaacaatttg gtcaaccagc tcatatgaaa gactatgtat tcactgaaaa agataatggt
     1561 ggcgtacata cgaattctgg aattccaaat aaagcagctt ataacgtgat tcaagcaata
     1621 gggaaatcta aatcagaaca aatttactac cgagcattaa cggaatactt aacaagtaat
     1681 tcaaacttca aagattgtaa agatgcatta taccaagcgg ctaaagattt atatgacgag
     1741 caaacagctg aacaggtgta tgaagcatgg aatgaagtgg gcgtggagta aaaatatata
     1801 aacaagaaga agtaatgtta aacacttata aataattaaa ttttaaatac agcttatata
     1861 tagggtatac ctacattgat aattgacttt gtaggtatac cctctctttg taattagatg
     1921 agaggagtgt gagggttgtc tgccgaaaga ctactcggca gtctaaaatc attacaagta
     1981 gtagatatgt gataattaaa tgctgactta gaatacaaaa ttcattttaa aagttgtcac
     2041 aaaaaattta catgtatttt tattatcttt tgcaaaacaa agtgttaaat tataaatgaa
     2101 acatgcatga atttattttt taatacaaga aacgtaacta ccaaaggagt ttacaatatg
     2161 aagaaaagta aacgattaga aattgtttct acaatagtta aaaagcataa gatttataaa
     2221 aaagaacaaa tcatttcata tattgaagaa tattttggtg taagatatag tgcaacaaca
     2281 attgctaaag acttgaagga actaaatata tatcgtgtcc ctatcgattg tgagacatgg
     2341 atttataaag ctattaataa tcaaacagaa caagagatga gagaaaagtt tagacactat
     2401 tgtgaacatg aagttctaag ttcaatcatc aatggttcat acattatcgt caaaacctca
     2461 cctggtttcg cccaaggcat aaactatttt atcgatcagc taaatataga agagatatta
     2521 ggtacggtga gtggaaatga cactacatta atcttaactg cctcaaatga tatggcagaa
     2581 tacgtatatg caaaattatt taaatagaca tgtatcaaat gaataataaa aatttgtttc
     2641 gtatcacgtg tactcaagtt agttaccaaa tattaacttg tgtacgcgtt tttttatgga
     2701 aagaaagaat tcatagtcat tcaattgact gtataaaaaa ctttatacaa catgttttta
     2761 tgggtatttt tgaataaaaa atgtatattt tgacccaaaa tacctttatt tatgtataaa
     2821 aatccattat tatgtattgt ataacaaaaa gatatgaaat tttcgacttt ctttatgtga
     2881 atataatcac atgtaagcgt ttgaagattg tctatactct aaatgaattc aaagataaaa
     2941 ggaggaaata gacatgacag atggtccaat taaagtaaat agcgaaattg gagctttaaa
     3001 aactgtgtta cttaagcgtc ctggaaaaga attagaaaat ttagtacctg attatttaga
     3061 tggattacta tttgatgata ttccatattt agaagtagct caaaaagagc atgaccattt
     3121 tgcgcaggtg ctaagagaag agggtgttga agtactttac cttgagaagt tagcagctga
     3181 aagtattgaa aatcctcaag taagaagtga atttattgat gatgtattag cagagtctaa
     3241 aaaaacaata ttaggtcatg aagaagaaat taaggcatta tttgcgacac tttctaatca
     3301 agaacttgta gataaaataa tgtcaggggt acgtaaggaa gaaattaatc cgaaatgtac
     3361 acatctagta gagtatatgg atgataagta tccattctat ttagatccaa tgccaaacct
     3421 ttattttact agagatccac aagcctcaat aggacacggt ataacaatca atcggatgtt
     3481 ctggagagca cgacgacgag aatcaatatt tattcaatat attgtaaagc atcatcctag
     3541 atttaaagat gcgaatattc caatctggtt agatcgagat tgcccattca atattgaagg
     3601 cggcgatgaa cttgttttat ctaaagatgt cttggctata ggcgtttcag aacgtacatc
     3661 tgcacaagct attgaaaagt tagcgcgacg tatttttgaa aatccgcagg cgacgtttaa
     3721 aaaagtagta gcaattgaaa ttccaactag tcgaactttt atgcacttag atacagtatt
     3781 tacaatgata gattatgaca aatttacaat gcattcagcc attttaaagg cagaaggcaa
     3841 tatgaatata tttattattg aatatgatga cgtaaataaa gatattgcca tcaaacaatc
     3901 tagtcattta aaagatactt tagaagacgt actaggtata gatgatatcc aattcattcc
     3961 aacaggaaat ggtgatgtca ttgatggtgc tagagagcaa tggaatgatg gctcaaatac
     4021 attatgtata agaccaggcg ttgtagtgac ttacgataga aactatgtat cgaatgattt
     4081 attgagacaa aaaggcatta aagtcattga aatatctggt agcgagttgg tacgtggacg
     4141 tgggggccct agatgtatga gtcaaccatt attcagagaa gacatttaac gtaataaatt
     4201 atagaaacga gggtgaaaat aatgacagaa attcaaaaac cgtatgattt aaaaggcaga
     4261 tcattattaa aagaaagtga ttttaccaaa gcagaattcg aaggacttat tgattttgca
     4321 attacattaa aagagtataa gaaaaacggt attaagcatc actacttatc tgggaaaaat
     4381 attgcactac tattcgaaaa gaattcgacg agaacgcgtg ctgcgtttac agttgcgtct
     4441 attgatttag gtgcgcatcc agaattttta ggaaaaaatg atattcaatt aggcaaaaaa
     4501 gaatctgtag aggatactgc gaaagtatta ggtagaatgt tcgatggtat tgaattccgt
     4561 ggtttttcac aacaagctgt tgaagattta gcgaagttct ctggtgtacc ggtgtggaat
     4621 ggattaacag acgattggca tcctacacaa atgttagctg attttatgac aataaaagag
     4681 aattttggat atctagaagg aataaactta acttacgttg gagatggacg taataatatt
     4741 gcgcattcat taatggtagc aggtgctatg ttaggtgtta atgtaagaat ttgtacacct
     4801 aaatcattaa atccaaaaga ggcatatgtt gatattgcaa aagaaaaagc gagtcaatat
     4861 ggtggttcag tcatgattac ggataatatt gcagaagcag ttgaaaatac agatgctata
     4921 tatacagatg tttgggtatc gatgggtgaa gaaagtgaat ttgaacaacg tattaattta
     4981 ttaaaagact atcaagtgaa tcaacagatg tttgatttaa caggtaaaga ttcaacgata
     5041 ttcttacatt gtttaccagc attccatgat acaaatacac tttatggaca agaaatttat
     5101 gaaaaatatg gattagctga aatggaagtt acagaccaaa tctttagaag tgaacattca
     5161 aaagtgtttg atcaagctga aaatagaatg catacaatta aggcagtaat ggcagcaaca
     5221 ttggggagtt aatcactaaa tggaacgata tgaatatgat gtgtctgatg atataagtgt
     5281 catgtacaga cacctcatat tggtattaaa ggagaaatga atatgaacga atcaggagat
     5341 aacaaactca gtaaatcttc tttaattgga ctagttatag gatccatgat tggtggcggt
     5401 gcgttcaata taatgtctga tatgggcggt aaagccggtg gattagccat tattattggt
     5461 tggattatta cagctatagg aatgatttca ttagcgttcg tatttcaaaa tttaaccaat
     5521 gaacggccgg agctagacgg tggtatttat agttatgctc aagcaggatt tggcgatttt
     5581 gtaggattta tcagtgcttg gggatattgg ttctcagcgt ttttaggcaa tgttgcctat
     5641 gcaacactat tgatgtcagc agtaggtaac tttttcccga tttttaaagg aggcaacaca
     5701 ttaccaagta ttattgtcgc ctcgttacta ctctggggtg tccatttctt gattttaaaa
     5761 ggcgttgaaa cagcagcatt tatcaatagt attgttactg ttgcaaagtt aataccgatt
     5821 ttacttgtaa tcatatgcat gataattgca ttcaattttg acacttttaa aacaggcttt
     5881 ttcagtatga cgtcagaggg tgtattgcca tttagttggg cgagcacaat gagccaagtt
     5941 aaaagtacga tgctagtgac agtttgggtg tttatcggta tcgaaggtgc agtaattttt
     6001 tctagtagag ctaaaaataa aaaagatgta ggtagtgcca cggttatagg acttatatca
     6061 gttttaatta tctatttctt attaactgta ttagctcaag gcgtgatttt gcaaaatcat
     6121 atttcgcaat tagattcgcc aagtatggca caggtgcttg caactattgt aggtggttgg
     6181 ggatctacac ttgtaaatat tggtttaatt atttcggtac taggtgcatg gttaggatgg
     6241 acactgcttg ctggtgaatt acctttcatt gttgcaaaag atggattatt tccaaaatgg
     6301 tttgctaaag aaaataaaaa tggagcacct gtaaatgcac tgcttattac caatatatta
     6361 gtacaattat ttttaataag tatgctattt acacagagtg cgtatcaatt tgcattttca
     6421 ctagcatcaa gtgctatttt atacccttac atgttcagtg cattttacca agttaaatac
     6481 actttagagc atcgacagca agcaactact aaacaatgga cgattggtat catagcctca
     6541 atttatgcta tatggcttat atatgcagca ggtatcaatt acttattatt gacgatgtta
     6601 ctttatattc cagctcttct tgtttataca atcgttcaaa agaataatca gacacgtttg
     6661 attaaatcag actatattct ttttatgatt attatcgtac ttgcagttat cgggttaatt
     6721 aagttattga tgggaacgat aaatgttttt taaaaggagc gacaaaaata tgaaagagaa
     6781 aattgtcatt gcattaggcg gtaatgcgat acagacaaca gaagcaacag ctgaagcaca
     6841 acaaacagct attagatgtg cgatgcaaaa ccttaaacct ttatttgatt caccagcgcg
     6901 tattgtcatt tcacatggta atggtccaca aattggaagt ttattaatcc aacaagctaa
     6961 atcgaacagt gacacaacgc cggcaatgcc attggatact tgtggtgcaa tgtcacaggg
     7021 tatgataggc tattggttgg aaactgaaat caatcgcatt ttaactgaaa tgaatagtga
     7081 tagaactgta ggcacaatcg ttacacgtgt ggaagtagat aaagatgatc cacgatttga
     7141 taacccaact aaaccaattg gtccttttta tacgaaagaa gaagttgaag aattacaaaa
     7201 agaacagcca gactcagtct ttaaagaaga tgcaggacgt ggttatagaa aagtagttgc
     7261 gtcaccacta cctcaatcta tactagaaca ccagttaatt cgaactttag cagacggtaa
     7321 aaatattgtc attgcatgcg gtggtggcgg tattccagtt ataaaaaaag aaaataccta
     7381 tgaaggtgtt gaagcggtta tagataaaga ttttgctagt gagaaattag caacgctgat
     7441 tgaagcagat accttaatga ttcttacgaa tgtagaaaat gtatttatta actttaatga
     7501 acctaatcaa caacaaatcg atgatattga tgtagcaaca ctgaaaaaat acgcggcaca
     7561 aggtaagttt gtggaaggat cgatgttgcc aaaaatagaa gctgcgatac gatttgttga
     7621 aagtggggaa aacaaaaaag ttatcattac caatttagag caggcatacg aagctttgat
     7681 tggtaataaa ggtacacaca ttcacatgta gcactttatc acgcgacaaa acattaaata
     7741 tgtttctccg ttgattcaaa tgaaaaagtt gtctgctgac actttgcaag gtttgaagga
     7801 gtttaactta tgacagaaaa ctttattttg ggtagaaata ataaattaga acatgaacta
     7861 aaggcattag cagattacat taatatccca tatagtatat tacaaccata tcaaagtgaa
     7921 tgttttgtca gacattatac gaaaggccaa gttatttatt tttcgccaca agaaagtagc
     7981 aatatttact ttttaattga aggtaacatt attagagaac attacaatca aaatggagat
     8041 gtatatcgtt attttaataa agagcaagta ttatttccaa tcagtaactt atttcatccg
     8101 aaagaggtta acgaattgtg tacagcatta accgattgta cagttcttgg attgcctaga
     8161 gaattgatgg cctttttgtg caaagctaat gatgatatat ttttgacact ttttgcatta
     8221 ataaatgata atgagcagca acacatgaac tataacatgg cattaacaag taaatttgct
     8281 aaagatcgaa ttatcaaatt gatatgccat ctatgtcaga cagtaggata cgatcaagat
     8341 gaattttatg aaatcaaaca gtttttaact attcaactca tgagtgatat ggctgggatt
     8401 tcccgggaaa cagctggtca tattattcat gaacttaaag atgaaaaact tgttgttaaa
     8461 gatcataaaa attggttagt aagcaaacat ttattcaatg atgtatgtgt ttaatataca
     8521 atgtaaaatg aataagttga acatgaggtc taacgtacat ttatacgtta ggcctttttt
     8581 gctagcatga tgaataattt aaaatgttag ttaaatttga ttgttgaaat tacagtaaaa
     8641 tttaaggtga tgaaaaattt agaacttcta agtttttgaa aagtaaaaaa tttgtaatag
     8701 tgtaaaaata gtatattgat ttttgctagt taacagaaaa ttttaagtta tataaatagg
     8761 aagaaaacaa attttacgta atttttttcg aaaagcaatt gatataattc
//
LOCUS       DAHHYJ010000112         8725 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000112 DAHHYJ010000000
VERSION     DAHHYJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.112"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(28..676)
                     /locus_tag="NBK22_002008"
                     /pseudo
     CDS             complement(28..676)
                     /locus_tag="NBK22_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(789..1337)
                     /gene="hxlB"
                     /locus_tag="NBK22_002009"
     CDS             complement(789..1337)
                     /gene="hxlB"
                     /locus_tag="NBK22_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCD2415190.1"
                     /translation="MAEFSDYQLILDELKMTLSHVEADEFSTFASKILHAEHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(1339..1971)
                     /gene="hxlA"
                     /locus_tag="NBK22_002010"
     CDS             complement(1339..1971)
                     /gene="hxlA"
                     /locus_tag="NBK22_002010"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCD2415191.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKEIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2048..2806)
                     /gene="nagB"
                     /locus_tag="NBK22_002011"
     CDS             complement(2048..2806)
                     /gene="nagB"
                     /locus_tag="NBK22_002011"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCD2415192.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQSQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFENEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            3082..3444
                     /locus_tag="NBK22_002012"
     CDS             3082..3444
                     /locus_tag="NBK22_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCD2415193.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQVTYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            3458..4123
                     /gene="bshB2"
                     /locus_tag="NBK22_002013"
     CDS             3458..4123
                     /gene="bshB2"
                     /locus_tag="NBK22_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCD2415194.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            4136..5014
                     /locus_tag="NBK22_002014"
     CDS             4136..5014
                     /locus_tag="NBK22_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCD2415195.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            5296..6786
                     /locus_tag="NBK22_002015"
     CDS             5296..6786
                     /locus_tag="NBK22_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD2415196.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINHTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITSDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(6910..8382)
                     /locus_tag="NBK22_002016"
     CDS             complement(6910..8382)
                     /locus_tag="NBK22_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD2415197.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLSGAGNGIKSFYKTVFNNLTRYKAIVV
                     STEKQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(8501..>8725)
                     /locus_tag="NBK22_002017"
     CDS             complement(8501..>8725)
                     /locus_tag="NBK22_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD2415198.1"
                     /translation="SDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSEN
                     NGSNNATLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         2823 a         1479 c         1349 g         3074 t
ORIGIN      
        1 ccatggtatt ctctgtttta acaaatttta taatactgta ttaatttctg atggatcatt
       61 gattataaaa tccggatttg aatgaagtaa tgaccttgca tcatgtgctc cccatgttac
      121 tgctgcagat ggtacaccag catgttgcgc catctcaaca tcaaatgttg aatcaccaat
      181 ataaaccgtt tgttggctat ttaaattgta gcgttgcaca attgtgtgta tgccttcagg
      241 atttggttta tatgcactta cttgatcgga tccaacagct tcggtaatca agtgatttaa
      301 tccaatagcc gataaatttc tttctaatac atcactcttc ttactagaca ccacgaaaag
      361 tttttttccc ttggttatac aaacttgtaa tggcttcagt tatacccgca aattcataaa
      421 tatagtcctt ttcaatagat tgatatgtat gtctaaatgt atcgattaac tttgctaatg
      481 ctgcttcatc taatggtcgg tctgctaatt ttaaaaatga ttcttcaata ggtattccca
      541 tataatgcgt tatttcttta gatgatggtt ccgttaagcc acatgcttta aatgcacttt
      601 gtgttgctac ttcaccacat tttttcgtgt ctgccaacgt accatcaaaa tcaaaaatat
      661 aattgtcaaa cttcaatatt aacaccccta ttcgtttatt attgatatta tactataatt
      721 tattatttaa ataaaaaaat aatcgataat atttctgcct aggcatcata ttatcgacta
      781 tctttatttt attctaaatt agcatgattt tgttgcatcg tttgttccgt aacattcatt
      841 tcagtcatca atcccattac aacactatct aaaaataatt gagatgcttg ttcaaacaaa
      901 cttcctaatg gttgtgccga gccttgttca tcatattttg tacctgcagg caaaacgatg
      961 ttcgtcccag ctagattgcc tattgcagaa tctttatttg tagtaattaa tacgatgtca
     1021 gcacctactg attttgcttt gtctgctaat aatcttaaat gttccgtgga acctgaacca
     1081 gagataatta caaatacatc attcgactta atcgcaggtg tcgttgattc tccaacaaca
     1141 tgtgcctgtt tgccgagctg atttaagcgc attgcaaaac tattcgccac gaatcctgaa
     1201 cgtcctttac cagctacaaa tatatgttca gcatgtagta ttttggatgc aaacgttgaa
     1261 aactcatccg cttcaacatg tgacaaagtc atctttaatt catctagaat taattgatag
     1321 tcactaaatt cagccatatt acttaccttc gattgcagcg cgacattgtt ttgcagcttc
     1381 tactggatca tctgcatttg cgattccgcc accaacaata acaagatcag gactttcagc
     1441 gacaatttct ttaattgtat ctggtttaat tccacctgct actgcaactt tagaattttt
     1501 aataacagat ttaacggttc ttaaactttc taatggtgat tgcccttctg cttgtaaatc
     1561 ataaccagtg tgtactgcaa tataatccgc acccatttca tctagttctt ttgcacgttt
     1621 ttctaaatct tgaacagcaa tcatatcaac tagtaattgt ttattatttt tatgagcttc
     1681 ttcaatagct gctttaattg atgcatcttc tgcaacacct agtattgtaa ttacatccgc
     1741 gccaaattta attgcttggc taacttcata atcagctgca tccataattt tcatgtctgc
     1801 taatactttt acattactaa tgttgtctgc catatgttta actgctggta aaccttcgtt
     1861 gtaaatgatt ggcgtaccga tttctacgat atctacataa tcttttactt tatttgctaa
     1921 ctcagccgcg tcttctttgt ttaataaatc aattgctaat tgtaattcca ctaaaaatca
     1981 tccttccaat gctttttatc tttctattgg aacctttccc gaggcatttc ttaattaaac
     2041 attgcaatca atccatttca tcgacatgaa ttttcgcaac atttttcggg caagcttcgt
     2101 cgtctaaata tatctcaaca ttcgggtgtt tgtgaagtaa tgtggctgga acatcaacag
     2161 aaatttcctg atttaataaa tgtgtaatag cagcacgttt cttttcacca aatgcgagta
     2221 aaatgatacg tttggcttga agaatattag caagtcccat cgaaatggct tgctttggaa
     2281 catcatcttc gttttcaaaa tatcgactat tagccttaat agtactttca gtcaaatcaa
     2341 cgatatgagt aacactatca aacggcgtac caggttcatt aaatccaata tgaccatttt
     2401 ctccaatacc taaaatttga atatcacgtt gactttgttg ttctaaaacg tcattatatt
     2461 ttgacgcttc cgcattcata tcatcggcat ctccatttgg aatatgaatg ttctttctat
     2521 taaaataagg atattgtttg aaaagcatgt catccatata atagtgataa ctttgcggat
     2581 gtgatgcggt taaacctaca tattcgtcta aattaaacgt ggatacattg tctacgttta
     2641 actgattttt atttaataac ttaacaagtt gctcatacaa atctgtcatt gtaccaccag
     2701 ttgctaaacc aagtttacag tgctgattaa atgccatctc tttatataac tcacatgcaa
     2761 catagaatga tgcttgtttt ttcgatccta agtttaatac tttcattgtt tacatgcctc
     2821 cacaaattta atacatttta aaaacttatg aattcattca tacaccatta atatagcatc
     2881 atttttaatg tttaacactt ttcaaattcc aacaaatttt tgatttttaa aagtaaaaaa
     2941 ctaatgctgt atgattgcgt aatcctgagt aattactctt catgaaaatt aatttataaa
     3001 taattgagca atcgtataca attattgaat agtttttagt aaaatagaaa gataaacaca
     3061 ttgatagtgt aaggagtgaa cgtgttggaa ccaattaaag aacaagaagt tatagattta
     3121 cttgcttctt ttgaacataa acctgtgtat ctgcatgttg aaacgacaaa tggggcctat
     3181 gcgaaccatt tcgatcaacg tgtttttaat gctggtacat ttttaagaaa catacaagta
     3241 acatatacgc atgcacaact aaaaggtggt aataaagaac cttatcgcat tggtcttaaa
     3301 ctatcaaatg gtggttgggt ttatgtacaa ggtctaacgc attttgaggt caatgaacat
     3361 gatgaatttt taattgcagg ttttaattac gaaggtcaac tcgctgcagc acttcaaatt
     3421 agtgagcgac catttaattt atagaggagg catcaacatg actgacgaaa gacatgtatt
     3481 agtaatattt ccacatcctg atgatgaaac cttctcatct gcaggcacat tagcaagtta
     3541 tatccaaaaa ggcatacctg taacatatgc atgtttaacc ctaggacaaa tgggcagaaa
     3601 tttaggtaat cccccatttg caacacgcga atcattacca agtattcgtg agcgtgaact
     3661 agaagaagct tgtaaagtta ttggtattac tgatttaaga aaaatgggtt tacgtgacaa
     3721 aactgtcgaa tttgaaccct acgaacatat agatggtatg attaaatctt taatcgatga
     3781 tacaaatcca tcattgatta tttcatttta tcctggatat gcagtacatc ctgatcacga
     3841 agcaacagca gacgctgtaa ttcgaacggt tgaacgcatg cctaaagaag aacgccctcg
     3901 tttaacttta gttgcgttca gtaatgatgc aaccgaagct cttggagaac ctgatattca
     3961 aaatgatatt acagatttta aagaacttaa aattaaggca tttgaagcac atgcttctca
     4021 aacaggtcca tttttaaaac aattagcaag tcctgaaatc gatggtaaac aacacagctt
     4081 tttaacagtc gaaccatatt ggacatatca ctttaaatct taacggagga aaaaaatgac
     4141 tgaatttgat ttatcgacac gagaaggtcg ttggaaacac tttggttccg tagatcctat
     4201 tgaaggcacg aaacctacta ctaagaacga aatgacagac ttacaaagta cacataaaga
     4261 ttttttattt gaaatagaag aagttggtat taaaaactta gtgtaccctg tacttgttga
     4321 ccagtatcaa acagctggca catttagttt ttcaacaagt ttaacaaaag acgaaaaagg
     4381 tattaatatg agccgtataa ttgaaagtgt tgaaaagcac tatgataatg gtattgaact
     4441 tgaatttaat acactttatc aggtgttacg cactttacaa acgaatatga aacaaaatgc
     4501 ggcaggcgtt gatgtttctg ggaaatggtt ctttgatcgc tatagtccaa ccacaaatat
     4561 taaagcagtt ggtaatgccg atgtcactta tggattagca attgacggtg ataaagttac
     4621 acgtaaagaa cttactattg aagcaactgt cacaacgcta tgcccttgtt caaaagaaat
     4681 aagtgagtat tcagcgcaca accaacgtgg tgttgtcact gttaaaacat atattaataa
     4741 agatcaagac atagttgatg actataaaaa taaaatttta gatgcaatgg aagctaatgc
     4801 aagttcaatt ctatacccta ttttaaaacg tcctgatgaa aaacgtgtga cagagcgtgc
     4861 atatgaaaat ccacgttttg tcgaagactt aattcgttta attgccgcgg acttagttga
     4921 atttgattgg ttagacggtt tcgatatcga atgtcgtaat gaagagtcta tccatcaaca
     4981 tgatgctttc gctaaattaa agtatcgcaa ataatatcat atgattaatt caaacgcgta
     5041 acattagttg cgcgttttta ttataccttc tagagttgta tatctctttt ataaatcttc
     5101 tcatttgatg tgcttgagtc ataaatcaat atcctaagct ctacaatgat atattggtcg
     5161 tagttgattg aataactact atcattataa atatcaaaat ttgttgtttt ataaataaac
     5221 acatcttcca gttaaaagaa gtaattaaat attatgtgat ataattataa aaaatagata
     5281 taagtggtgt tgtaaatgaa ttattttgta ggtaatagtt taggtgttaa tttaactggg
     5341 atagagaaag caattattaa tcgtttaaat ttatttaaag aaatgggaag accagcgcaa
     5401 tgtgtatttc tgtcatggaa tagatattta tatagaaatg cccaaaatta tattactagt
     5461 tctgactata ttaatatgta tgatttcttc caagaagcaa catatttaga acgcaatgaa
     5521 ccatttgact ggttgtcgta ttggactgat gaatgtcatt atactttaaa acatgttgaa
     5581 aatagtcatg actttagaat atatgatcaa gagcgtttct taatgtatgc ccattttcaa
     5641 gatcctaaat atcgtatatt agattatgta aatcattttg atagtcaaag acgaaaagta
     5701 aaacgtgatt tttatgatgt gcgtggcttt ttaagttgta gtaggattct tgttgataaa
     5761 caacaaacat tatgtgaatt tttctataat ccagaaggtg acacaaaatt agaaaagtat
     5821 ttttcttaca aggatggcaa accagaagta caaaaaatta ttgtttacta tgctaacaaa
     5881 caatatttct ttaataatga aacggaatta ggcgcatttt tcattaaaca actatatcaa
     5941 catggggatt tatttttcag tgatcgtaat gtgtacacag cacctatttt caacttaacg
     6001 cctgagtcaa tcccagtagt agctgtgctt cacagtacac acattaaaaa tattgatgca
     6061 ttagattcat cgccatttaa aaatgtatat aaagcgatgt ttgaaaattt atctcgatat
     6121 cgtgcaatta tcgtttctac tgagcagcaa aaattagatg ttgaaaaacg tattaaccat
     6181 actattccag ttgttaatat tcctgtcggc tatagtgaaa caattgatac accagtccaa
     6241 acattagatc agcgcagtgt taaattaatt tccgttgctc gttattcacc tgaaaaacaa
     6301 ttacatcaac aaattgaatt aattaaacgc ttagtatcgt atgtcccaaa aatagagctt
     6361 catatgtatg gttttggttc tgaaagtaaa aaattaaatg aattgataca aaaatatgga
     6421 ttggaaaatc atgtttactt acgcggcttt ttaagcaatc tagaccaaga atatagcgat
     6481 gcctatttga gtttaataac aagtaacatg gaaggtttct ctttagcgct acttgaatct
     6541 ttagcacatg gtgttccagt tattagttat gatattaaat atggacctaa cgaactgatt
     6601 acttcagatt tcaatggtta tttaattact aaaaatgatg aagatgccct atttgataaa
     6661 gtaaaatatg tgattgatca tcctgaagta cagcaacgac tgtcaaaagg tagtttggca
     6721 aaagcacaac aatatagtaa agcgtcgtta attaaacaat gggaccaatt tgttcgtctt
     6781 atataaatgt actttacctt tatttttaca aaaatagcat tttcctatgt catttaacta
     6841 aacatgtaag ttcgtatgaa cgaggtttgt taaatagatg attctaggaa aatgcttttt
     6901 tcttttgact tagtttaaaa tattttgcca ctttgtactg atagtagttg cattgtactg
     6961 ttgtgcagat tctatgctat taattgaaaa ctgttgcaat ttttgagtat tatttagtaa
     7021 ttggtcgacc ttttcaacca tttgattgat gtcaccttga ggtactaaat agccattaaa
     7081 tccatcttga atcagttctg atggaccata atctacatca taactgatca ctggtgtacc
     7141 tactgaaagc gactctaaaa ttgctaagcc aaaaccttcc attttacttg tcgataacat
     7201 cagttctgct ttagcaatct cttcattaat atgcgtctta aaaccatgaa atttaacatg
     7261 ttccgataaa tgataatctt ctacaagttg tcgatattct gacaaaccat ttccatgtcc
     7321 ataaatattc aattgaatat tgggatgttt tgttactaat tgcttgatta cttcaatttg
     7381 atgtttaatt tgtttatttt caacgaggcg agcaattgat atgatatgat ttttctcctt
     7441 ttgattgatg tcaaattgat actttaaatt tgccacgtag ccaaccggaa tattgataac
     7501 tggtatttta ttttcaatat attgtgaaat atcttggcat tgcttttctg ttgatacaac
     7561 aatcgcttta taacgtgtta aattattaaa cactgtttta taaaaacttt ttataccatt
     7621 accggcaccg gataaatgtg tactatggag cacaacaata actggaatac tttgatttaa
     7681 tcccgctata acatttccta attcatgagg acgatctaat atgatttgat cattattttt
     7741 acataattga tggagaaaat attgaactaa ttcatcttct gtatcaaaaa attgttgatg
     7801 ctggtcttca tttaagataa cctttgtgag cgtgtttttc ccttttatat cgtcaaaata
     7861 tttttggatg acgatttccc cattaggtgt atagtaattt tcgagtacaa tccgttgtcc
     7921 ttcacctaaa attcgagaac aacttaaaaa gcctcttcca tcatacaatt cgcgttttac
     7981 ttttcttctt ttatgatcaa aataattcac ataatttaat tgatgatact gtttatctaa
     8041 aaaatgagca tacattacaa attgctcttc atcatatatt ctgacatcat ttgaattttc
     8101 cacaaatttc aatgtgtacc tacatgactt ttcccaatac tgtatccagt taacttgctt
     8161 tgtcttttta taattgattg ctttttgaaa atagtcatac attgtaaata catcattttc
     8221 aatctgatgt tgcttcgcat atgtgtatga ataaggattc catttaacat atacacattt
     8281 tgaagatatg ccgtgttgtt tgaacaactt caatctattt atttgcgctt tttctacacc
     8341 tgtaatttta ctttctaaaa ttgttcctaa aatgtaattc atattatcgc ctcatataag
     8401 ttttattccg tatctttatt gtttatttta tatgaaaaat acatctattg catgtgtaat
     8461 tataaaaaaa ccaggccaca aggacctggg tcatattgta ttatttgttt tgttttttgc
     8521 gacgaccgaa taacaataat gaacctaatg ctgcaaataa tccaccaaat aacgttgcgt
     8581 tatttgagcc gttattttca ctacctgttt ctggtaatgc ttttgcttta ttgtgatggt
     8641 ctttagtagt actcattggt ttaacaggtg tatgttttcc tgcatccgag tccgaatcgc
     8701 tatctgagtc tgaatcactg tctga
//
LOCUS       DAHHYJ010000113         8681 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000113 DAHHYJ010000000
VERSION     DAHHYJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.113"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            81..857
                     /gene="modA"
                     /locus_tag="NBK22_002018"
     CDS             81..857
                     /gene="modA"
                     /locus_tag="NBK22_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD2415199.1"
                     /translation="MKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQVAA
                     AASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVDAL
                     KDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQYLD
                     NNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEVEL
                     KKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            871..1542
                     /gene="modB"
                     /locus_tag="NBK22_002019"
     CDS             871..1542
                     /gene="modB"
                     /locus_tag="NBK22_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCD2415200.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            1543..2148
                     /locus_tag="NBK22_002020"
     CDS             1543..2148
                     /locus_tag="NBK22_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2415201.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWKIPIIFVTHSNYEAEQMAHEIITIG"
     gene            2318..3322
                     /locus_tag="NBK22_002021"
     CDS             2318..3322
                     /locus_tag="NBK22_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCD2415202.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            3353..3859
                     /locus_tag="NBK22_002022"
     CDS             3353..3859
                     /locus_tag="NBK22_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCD2415203.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDFDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(3861..4355)
                     /gene="moaC"
                     /locus_tag="NBK22_002023"
     CDS             complement(3861..4355)
                     /gene="moaC"
                     /locus_tag="NBK22_002023"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCD2415204.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            4418..5677
                     /locus_tag="NBK22_002024"
     CDS             4418..5677
                     /locus_tag="NBK22_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCD2415205.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVRDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQQDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            5674..6159
                     /gene="mobB"
                     /locus_tag="NBK22_002025"
     CDS             5674..6159
                     /gene="mobB"
                     /locus_tag="NBK22_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCD2415206.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKEDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVVVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            6173..6619
                     /locus_tag="NBK22_002026"
     CDS             6173..6619
                     /locus_tag="NBK22_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCD2415207.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAVSSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            6625..6858
                     /gene="moaD"
                     /locus_tag="NBK22_002027"
     CDS             6625..6858
                     /gene="moaD"
                     /locus_tag="NBK22_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCD2415208.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            6865..7464
                     /gene="mobA"
                     /locus_tag="NBK22_002028"
     CDS             6865..7464
                     /gene="mobA"
                     /locus_tag="NBK22_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCD2415209.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAKVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFKYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            7484..8506
                     /gene="moaA"
                     /locus_tag="NBK22_002029"
     CDS             7484..8506
                     /gene="moaA"
                     /locus_tag="NBK22_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCD2415210.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDPVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVANRQRKKIN
                     MNYIGG"
BASE COUNT         3058 a         1212 c         1748 g         2663 t
ORIGIN      
        1 aacaggcaac ggttcttttc aaatataata gtaagtgtat aatgaaaatg taaatattta
       61 ataattgggg gtatttcata atgaaacgtt tatttgctgt tgtgattgca atgcttttag
      121 tattagctgg ttgctctaat tctaacgata ataatgaaag taaaaaagat gacgcagaca
      181 atggtaagaa acaagagatt caagttgcag cggcagcaag tttaacagat gtaaccaaga
      241 aattagcttc agaatttaaa aaagagcata aaaatgctga tattaaattt aactatggtg
      301 gatcaggggc attaagaaaa caaattgaat caggcgcacc tgtcgacgta tttatgtctg
      361 caaatactaa agatgtagat gcattaaaag acaagaataa agcgcatgat acatataaat
      421 atgcgaaaaa tagtctagta ttaattggtg ataaagattc aaattacact tcagtaaaag
      481 acttaaaaga caatgataaa ttagcattag gtgaagtgaa aactgtacca gcaggaaaat
      541 atgcgaaaca gtatttagat aacaataact tatttaaaga agtcgaaagt aaaatcgttt
      601 atgctaaaga tgtaaaacaa gtattaaatt atgttgaaaa gggtaatgcg aaacaaggtt
      661 ttgtgtataa aactgactta tataaacaaa ataaaaaaat tgatactgta aaagtaatta
      721 aagaagtaga acttaagaag ccaatcacat acgaagctgg tgctacatca gatagtaaat
      781 tagcaaaaga gtggatggaa ttcttaaaat cagataaagc taaagaaata ctaaaagaat
      841 accactttgc agcataagga gttgtaatcc atgcctgact taacaccttt ttggatatca
      901 atacgagttg ctgtaatcag tacgattatt gtaacggttt taggtatttt tatatctaaa
      961 tggttgtatc gtcgtaaagg ttcgtgggtt aaagtattgg aaagtttatt gatattacct
     1021 attgttttgc cgccaacggt attaggtttt attctattaa tcatcttctc gccaagagga
     1081 ccaatcggtc aattctttgc gaatgtacta catttacctg tagtgttcac tttgacaggt
     1141 gctgtgatag catctgtcat tgttagtttt ccactaatgt atcaacatac tgtgcaaggc
     1201 ttcagaggta tagacacgaa aatgattaat acagctagaa caatgggagc aagtgaaacg
     1261 aaaattttcc tcaaattaat tttaccatta gctaaacgct ctattttagc aggtataatg
     1321 atgagttttg ctcgtgcatt aggtgagttt ggtgctacat taatggttgc aggatatatt
     1381 ccaaataaaa cgaatacact acctttagaa atatacttct tagtggaaca aggtagagaa
     1441 aatgaagcgt ggttatgggt attagtgcta gtcgcattct ctattgtggt tatatctaca
     1501 attaatttat tgaataaaga taaatataag gaggtcgact agatgcttaa aatcaatgtg
     1561 aaatatcaat taaagaacac tttaattcgc atcaatatag atgatactga accaaaaatt
     1621 tatgcagttc gtggtccatc tggcattggt aaaactactg ttttaaatat gattgccgga
     1681 ttacgtaaag cagatgaagc tattatcgaa gtgaatgggc aattacttac tgatacggca
     1741 aaaaacgtga atgttaaaat tcaacaacga cgtattggat atctgtttca agactaccaa
     1801 ttgtttccta atatgacggt ctataaaaat attactttta tggctgaacc atctgaacac
     1861 atcgatcaat taattcaaac tttaaacatt gatcatttga tgaaacaata tcctatgaca
     1921 ttgtcaggtg gagaggcaca acgtgtagca cttgcacgtg cacttagcac gaaaccagat
     1981 ttaattttat tagatgaacc tttttctagt ttggatgata ctacaaaaga tgagagtatt
     2041 acattagtta aacgtatttt caacgaatgg aaaataccaa tcatatttgt gacacattca
     2101 aactatgaag cagaacaaat ggctcatgaa attattacaa ttgggtaatc atttatttgc
     2161 cattaaagag tttagaacgt atttaaaatt gtagaagtga atgcttctat cagcatttta
     2221 atgatgtttt aaactctttt ttaggggcag tttttttgag agacattgac gcgcgtcata
     2281 taatgaaagt aatgataaaa agaaaggata acttaatgtg agtcaagaac gttattcaag
     2341 gcaaatttta tttaaacaaa taggtgaaat aggtcaaagc aaaataaatc aaaaatgtgc
     2401 gttgattatt ggtatgggcg cattaggtac acatgtggcc gaaggacttg ttagagcagg
     2461 cattgctaaa ctaatcattg ttgatagaga ttatattgaa tttagtaatt tacaaagaca
     2521 aacattgttt actgaagaag atgctttgaa aatgatgcct aaagtggttg cagctaaaaa
     2581 gcatttgcta gcgttacgta gtgatgttga tattgatgat tatattgccc atgtggatta
     2641 ttattttttg gaaacacatg gacaggacgt tgacgttatt attgatgcaa ccgataactt
     2701 tgaaacacga caactgatta atgattttgc atataaatat cgtatacctt ggatttatgg
     2761 tggtgttgta cagagtacat atacagaagc tgcatttata cctggtaaaa caccttgctt
     2821 taactgtttg gtaccacaat tgccagcatt aaatttaaca tgtgatacag taggggtcat
     2881 tcaacctgcc gtgacgatgg caacaagttt acaattaaga gatgcgatga aagtattaac
     2941 ggaacaacca attgacacaa aaataactta tggcgatatt tgggaaggta gtcattattc
     3001 atttggtttc agtaaaatgc aacgttcaga ctgtacaact tgtggagatg taccaagtta
     3061 tccttattta aacaagaatg aacaacgtta tgcaacattg tgtggtagag acactgtaca
     3121 gtatgaaaat gcatcaatta cacacgacat tcttgttcaa tttttaaaac aacatcaact
     3181 aaattatcgc agtaattcgt atatggttat gtttgaattt aaaggacacc gcattgttgc
     3241 ttttaaaggt ggaaggtttt taatacatgg catgacacgc acatcagatg ccacacatct
     3301 aatgaattta ttgtttggat aaaaaaagat aagacaaaag gagtgtaata ttatgggcga
     3361 acatcaaaac gttaaattga atcgtacagt taaagcagcc gtactaacgg tatcagatac
     3421 tagagacttt gatacagata aaggtggtca atgcgtgcgc caactattac aagcagatga
     3481 cgttgaagtg agtgacgcac attatacaat tgtgaaagat gaaaaagtag ccatcacgac
     3541 gcaggtgaag aagtggttag aagaagatat tgatgtcatc attacgactg gtggaacagg
     3601 tattgcacaa cgtgatgtga cgattgaagc agtaaaacca cttttaacta aagagataga
     3661 aggctttggg gaattgttta gatatttgag ttatgttgaa gatgttggca cgcgtgcatt
     3721 attgtctcgt gctgtagcag gtacagttaa taataaattg atattttcga ttccaggatc
     3781 aacaggcgca gttaaattag cattagaaaa gctcattaaa ccagaattaa atcatctgat
     3841 tcatgagctt acaaaataat ttattgattt gattggcgtt gaaaatctcc agatttaccg
     3901 ccagacttgc tttcaaggta ggtttcgcca ataatcatac ctttatcaac tgctttcgtc
     3961 atgtcgtaaa tggttaaagc cgttgctgat gcagcggtta aagcttccat ttcaacaccg
     4021 gttttgccag ttgtagagac agttgtttga atgtttaaag tataaagggg tgcatttgtt
     4081 tcatcccagc tgaagtgaac atctatgcca gtcaatggta atggatggca catcggaata
     4141 attgttgatg tatttttggc agccataata ccagcgattt gagcagtgtt caatacattg
     4201 cctttggtat tggtgttatt agaaatctgc ttaaaaatag tttcatttac agtaatgctt
     4261 gaatgagcga cagcagttct ttttgtaatt tgtttgtctg atacatcgac cattttggcg
     4321 tggccttgtt gattaatatg agtaaactca gtcattttac ccctcctagt gcatctagta
     4381 tatcatgaaa aaataaaagt tttggagatg atttttaatg gtagtagaaa aaagaaaccc
     4441 aatcccagtt aaagaagcaa ttcaacgtat cgttaatcag cagagttcaa tgccggcaat
     4501 tacggtagca cttgaaaaaa gtctaaatca tatcttagca gaagatattg tagctactta
     4561 tgatatacca aggtttgata aatcacctta tgatggtttt gcaattcgca gtgttgattc
     4621 acaaggggca agtggtcaga atcgcattga gtttaaagtg attgatcata ttggtgcagg
     4681 ttcagtttct gataaattag ttagggatca cgaagcggtg cgtattatga ctggagcaca
     4741 aatacctaat ggcgcagatg ctgttgttat gtttgaacaa acgattgaac tagaagatac
     4801 atttacaatt cgtaaaccat tttcaaaaaa tgaaaatata tctttaaaag gtgaagaaac
     4861 aacgacaggc gatgttgttc taaaaaaagg acaagtaatt aatccagggg ctatagcggt
     4921 ccttgcaaca tatggctatg cagaggttaa agttattaag caaccgagtg tcgctgttat
     4981 tgcaacagga agcgaattat tagatgttaa tgatgtatta gaagatggaa aaattcgtaa
     5041 ctctaatggc ccaatgattc gtgccttagc agaaaaatta ggtcttgaag ttggtattta
     5101 caaaacacaa caagatgatt tagatagtgg catacaagtc gttaaagaag ctatggaaaa
     5161 acatgatatc gttattacaa cgggaggagt ttctgttgga gattttgact atttacctga
     5221 gatttataag gctgtaaagg cggaagtgtt atttaataaa gtagcaatgc gtcctggtag
     5281 cgtaacaacg gttgcatttg cagatggaaa gtatttgttt ggattatctg gaaatccatc
     5341 agcttgtttt acaggatttg aactatttgt gaaaccagct gttaaacata tgtgtggcgc
     5401 actagaagtc ttcccgcaaa taattaaagc aacattaatg gaagatttta ccaaggcaaa
     5461 cccattcaca cgatttatac gtgctaaagc aacgttaaca agtgctggag ctactgtagt
     5521 accttcagga ttcaataaat caggtgcggt tgtagcgatt gcacatgcta actgtatggt
     5581 catgttacca ggagggtcac gtggttttaa agcggggcat acagtagata ttatattgac
     5641 tgaatctgac gctgctgaag aggaacttct tttatgattt tacaaattgt aggttacaaa
     5701 aagtctggta agacaacatt gatgaggcat attgtctctt tcttaaagtc acatggttat
     5761 acagttgcta ctattaaaca tcatgggcat ggtaaggaag atattcaatt acaggattca
     5821 gacgtcgatc acatgaagca ttttgaagcg ggggcagatc aaagtattgt acaaggtttt
     5881 caatatcagc aaactgtaac acgtgtagat aatcaaaatc ttactcaaat tattgaaaaa
     5941 tctgttacaa ttgacaccaa tatcgtatta gttgaaggct ttaaaaatgc tgattttgaa
     6001 aaagtcgtag tctatcgaaa tgaagaagag ttgcaagtat tacaacaatt gtcgaatgtt
     6061 tgttatagca ttaatgtaag ggagcatgaa gattttacag catttgagca atggttatta
     6121 aataaaatta aaaatgattg tgatacacaa ttaacataga ggattgaaat gaatgaaaca
     6181 atttgaaatc gtgacagaac cgatacaaac agaacaatat cgtgaattca ctataaatga
     6241 atatcaaggt gcagtagttg tttttaccgg tcatgttcgc gaatggacta aaggcgtcaa
     6301 aacggaatat ttagaatatg aagcgtatat tccaatggct gaaaagaaat tggcacaaat
     6361 tggagatgaa ataaatgaaa aatggcctgg aacgataacg agtattgttc atagaatagg
     6421 gccattacaa atttcagata tcgctgtatt aattgcagtt tcttcaccgc atcgtaaaga
     6481 tgcctatcga gcaaatgaat atgcaattga gcgtataaaa gaaattgttc cgatttggaa
     6541 aaaagaaatt tgggaagatg gttcaaaatg gcaagggcat caaaaaggga attatgaaga
     6601 agcgaagagg gaggaataag agagatgaag gtactttact tcgcagaaat taaagatata
     6661 ttacaaaaag cacaggaaga tattgtgctt gaacaagcat tgactgtaca acaatttgaa
     6721 gatttattgt ttgaacgtta tccgcaaatc aataataaaa agtttcaagt tgctgtaaat
     6781 gaggaatttg tacaaaaatc ggatttcatt caacctaatg atactgttgc attaattcca
     6841 ccggttagtg gaggttaagg gagcatgaaa gcaataattc ttgcaggtgg tcattcagtg
     6901 cgatttggta agcccaaagc ttttgcgaaa gtgaacggtg agacctttta tagtagagta
     6961 attaagacat tagaatcaac aaatatgttc aatgaaatta ttattagtac aaatgcgcaa
     7021 ttggcaacgc aatttaaata tccaaatgtt gttatagatg atgagaatca taatgataaa
     7081 ggtccattag caggaattta tacaatcatg aagcaacatc ctgaagaaga attgtttttt
     7141 gtcgtttctg ttgatacacc aatgattact ggtaaagctg taagcacgtt gtatcagttt
     7201 ttagtttctc atcttattga aaatcattta gatgtcgcag cttttaaaga agatggacgt
     7261 tttattccaa caattgcatt ttatagtccg aatgcattag gcgctataac taaagcacta
     7321 cattctgata attacagttt taaaaatgta tatcatgaat tatcaacgga ttatttggat
     7381 gtcagggatg tagatgcgcc ctcatattgg tacaaaaata taaattatca gcatgatttg
     7441 gacgctttaa ttcaaaaatt gtaagctgtt aggaggtcca caaatggtag aacaaataaa
     7501 agataaacta ggacgtccca tccgtgactt acggttatct gtgacagatc ggtgtaactt
     7561 taggtgtgat tattgcatgc ctaaagaggt atttggagat gatttcgtat ttttacctaa
     7621 aaatgaactt ttaacgtttg atgaaatggc tagaatcgct aaggtatatg cagaattagg
     7681 tgtaaaaaaa atacgcatta caggtggaga accattgatg cgacgggatt tagatgtact
     7741 tatagctaaa ttaaatcaaa tcgatggtat tgaagatatt ggtttgacta caaatggttt
     7801 gttattaaaa aagcatggac aaaagttata tgatgctggg ctacgcagaa ttaatgtcag
     7861 tttggatgct attgatgata cgctatttca atcaatcaat aatcgtaata ttaaagcgac
     7921 tacgatttta gaacaaattg attacgcgac gtctattggt ttgaatgtaa aagtaaatgt
     7981 tgttatacaa aaaggtatta acgatgatca aatcatacca atgcttgaat attttaaaga
     8041 taaacatata gagattcgat ttatagaatt tatggatgtt ggtaatgata atggatggga
     8101 tttcagtaaa gttgtaacta aagatgaaat gcttacaatg atagagcagc actttgaaat
     8161 cgatcctgta gaaccaaaat attttgggga agtagcaaaa tattatcgcc ataaggataa
     8221 tggtgttcaa tttggtttga ttacaagtgt ttcacaatca ttttgttcta catgtacacg
     8281 cgcaaggctg tcatcagatg ggaagtttta cggatgttta tttgcaactg tcgatggatt
     8341 taacgttaaa gcgtttattc gttctggcgt gaccgacgaa gaattaaaag aacaatttaa
     8401 agctttatgg caaataagag atgatcgata ttcagatgag agaactgctc aaacagttgc
     8461 caatcgtcaa cgtaaaaaga taaacatgaa ttatattggt ggttaatgtg tagggaccac
     8521 tatatattaa atcattagag attctttaat atttctgtct tactccctaa aatacaatat
     8581 tatttattaa agtaaaaacg gtcatatcta tgccagattt aatagaaatg atcgttttta
     8641 aagtttttac aaattggcgg ggccccaaca tagaagctga c
//
LOCUS       DAHHYJ010000114         8651 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000114 DAHHYJ010000000
VERSION     DAHHYJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.114"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            23..520
                     /locus_tag="NBK22_002030"
     CDS             23..520
                     /locus_tag="NBK22_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415211.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
     gene            977..2044
                     /locus_tag="NBK22_002031"
     CDS             977..2044
                     /locus_tag="NBK22_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415212.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYITLLLDFNKIVRALGIVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYTIMGYVGLF
                     IVVAVIIKYFKRKNADKKHIA"
     gene            2195..3238
                     /locus_tag="NBK22_002032"
     CDS             2195..3238
                     /locus_tag="NBK22_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD2415213.1"
                     /translation="MNKDNKWTMITALFITVISVLLAFHLKQHYDQITNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
                     DSAGAQLASQFTAIQTNDRLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELPLNPLN"
     gene            3567..3995
                     /locus_tag="NBK22_002033"
     CDS             3567..3995
                     /locus_tag="NBK22_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415214.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(4204..4710)
                     /locus_tag="NBK22_002034"
     CDS             complement(4204..4710)
                     /locus_tag="NBK22_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2415215.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            4823..5746
                     /gene="graX"
                     /locus_tag="NBK22_002035"
                     /gene_synonym="apsX"
     CDS             4823..5746
                     /gene="graX"
                     /locus_tag="NBK22_002035"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCD2415216.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFTISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQIDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTPVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWMGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            5762..6436
                     /gene="graR"
                     /locus_tag="NBK22_002036"
                     /gene_synonym="apsR"
     CDS             5762..6436
                     /gene="graR"
                     /locus_tag="NBK22_002036"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCD2415217.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            6429..7469
                     /gene="graS"
                     /locus_tag="NBK22_002037"
                     /gene_synonym="apsS"
     CDS             6429..7469
                     /gene="graS"
                     /locus_tag="NBK22_002037"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCD2415218.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDD
                     YVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEI
                     VERMSEVTNLSF"
     gene            7613..8374
                     /gene="vraF"
                     /locus_tag="NBK22_002038"
     CDS             7613..8374
                     /gene="vraF"
                     /locus_tag="NBK22_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCD2415219.1"
                     /translation="MAILEVKQLTKIYGTKKMAQEVLRDINMSIEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGKKLEKLSNKELSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKEHMLNRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLTRMNEAFKSTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGVNYDL"
     gene            8364..>8651
                     /locus_tag="NBK22_002039"
     CDS             8364..>8651
                     /locus_tag="NBK22_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415220.1"
                     /translation="MTFNEIIFKNFRQNLSHYAIYLFSLITSVVLYFSFVALKYAHKL
                     NMTESYPIIKEGSQVGSYFLFFIIIAFLLYANVLFIKRRSYELALYQTLGLS"
BASE COUNT         3143 a         1140 c         1519 g         2849 t
ORIGIN      
        1 ggagaaatag gaggacattg ccatgcaaca ccttataaaa aaacatgtat tgaatggcga
       61 gtttgattta gtacgacaat tgatgtccga aacagatttt atggaatttg aagaagcata
      121 tatttcaagt gcgcatgaag tagaaagtat gatgttttat acatgtattt tagatatgat
      181 taagtacgaa gaatcatctg aaatgcatga cttagcattt ttattgcttg tgtatccact
      241 aagtgaatat gaaggtgctt tggattctgc ttattatcat gcagacgctt ccataaaact
      301 tactgacggc aaagaagtta aaagtttgtt acaaatgtta ttattgcatg cgataccaac
      361 acctgttatt tcagataaga aggcttttga tatcgccaag caaattttaa aattagatcc
      421 taataataat gttgctcgta acgtcttaaa agacactgcc aaacgtatgg acaacgttgt
      481 tgttgatata aatgaattac accaacgtaa tgcacgttaa ttacatttca attatattag
      541 cttaataata gttttaacat ttggttgggt tgggacatat gttccagcct tttttaatac
      601 ttaaaaacta acgaagtata cttgtgtgca caaatggttt ttatacaaca ttttataaat
      661 ttatacattt taataaagaa catacgatag atggtttaaa ccttgttaac tgagaaattt
      721 tgatatgtat tcttcgaaat ttaactaaat atacgaaatt caagaagcac aataattaat
      781 catttttcct atacaaaagt tcgtatgact gcattataaa agcataaatt tataattttt
      841 ttaaatgtca ttgaacgtga taatgtgaat ggattgagca attttgaaaa agtgaaaaat
      901 aacctatgcg acttgcaatt aattttcagt acgttataat gcacactgtg caaaattaag
      961 gaggtctatt attcacatga tgatgaataa agaagcaaca aaaattggat ttgcctacgt
     1021 cggcattgta gtgggcgcag gattttcaac tggacaagaa gttatgcaat ttttcactaa
     1081 atatggcttg tgggcttatt taggtgttat tatatctggt tttattttag cttttattgg
     1141 gcgccaagta gcaaaaattg gtactgcctt tgaagcgaca aatcatgaat caacattaca
     1201 atacgtattc ggtgaaaagt ttagtaaagt ctttgattat attttaatct tcttcttatt
     1261 tggtatagct gtaaccatga tagctggtgc aggcgcaaca tttgaagaaa gttataacat
     1321 acctacatgg ctaggtgctt taattatgac attagcgatt tatattacgt tgctattaga
     1381 ctttaataaa atagtacgtg cactaggtat cgttacacca tttttaattg ttttagttgt
     1441 attaatcgct ggcgtttatt tatttaaagg tcatgtttca ttagcagaag ttaaccaagt
     1501 agtgcctgaa gcaagtattt ggaagggaat ctggtttggt acaatatatg gtggattagc
     1561 tttttctgta ggttttagta ccatcgtagc aatcggtggg gatactgaaa agcgtacagt
     1621 gtcaggtgca ggcgcgatgt atggtggtat tatctatact gtattactag cattgatcaa
     1681 ctttgcattg caaagtgaat atccaactat taaaaatgcc tcaattccta cattgacgtt
     1741 agcaaataat atccatcctt taatagcaac agtgttatct gttattatgc tggcggttat
     1801 gtataatact attctaggac taatgtattc atttgcagca cgttttacag aaccatacag
     1861 taaaaattat catatcttta ttattataat gatggtagca ggttatttat taagtttcgt
     1921 aggatttgct gaattaatta ataagttata tacaattatg ggatatgtag gcttatttat
     1981 tgtagtagct gtaattatta aatatttcaa acgtaaaaat gcggataaaa aacatattgc
     2041 ttaatatcat atgagggata tccgaaactt tacaattgaa tcactttgtt ttaaccttaa
     2101 aagcaattcg tctctactct tatcgggcga attgcttttt atatttattc agtctattaa
     2161 tatgagcgtc taacaaatag agaggtacga tgtaatgaat aaagataata aatggacgat
     2221 gataactgcg ctttttataa ctgtaatcag tgtattgtta gcatttcatc tgaaacaaca
     2281 ttatgaccaa attacaaatg agaaccatgc taataaagac aaaattaata ttaaaaataa
     2341 aaatgtgcgc atttatcaaa accttacata caatagagtt ttccctaaca gtaaattaga
     2401 tattattaca cctgttgata tgtcttctaa tgccaaactg ccagttattt tttggatgca
     2461 cggtggtggt tatattgcgg gtgataagca gtataaaaac ccattattag cgaaaattgc
     2521 tgaacaaggg tacattgttg tgaatgtaaa ttatgcattg gcgccacaat ataaatatcc
     2581 cacaccatta attcaaatga atcaagcaac tcaattcatt aaagaaaata aaatgaattt
     2641 acctattgat tttaatcaag taattattgg cggtgattct gcaggtgctc aattagctag
     2701 ccaatttacg gcaatacaga cgaatgatcg cttaagagaa gccatgaaat ttgatcagtc
     2761 attcaaacca tcgcaaatta aaggtgctat actatttggt ggtttttata atatgcaaac
     2821 agttagagaa actgagtttc caagaataca gttatttatg aaaagttata ctggcgaaga
     2881 agattgggaa aagagcttta aaaatatttc acaaatgtcg acagtaaaac aatcgacaaa
     2941 aaattatcca ccaacatttt tatctgttgg agatagcgat ccattcgaaa gtcaaaatat
     3001 agaattcagt aagaaattac aagaattgaa tgtaccagta gatactttgt tttatgatgg
     3061 tacgcatcat ttacatcatc agtatcaatt tcaccttaat aaacctgaat cgatagataa
     3121 tatcaaaaaa gtgttacttt tcttaagtcg taatacatct tctagtggta ttcaaactga
     3181 agagaaacca caaatagaaa atccgagtaa tgaattaccg ttaaatcctt taaactaatg
     3241 ataaacagta gtaatttatt acttaagcaa catttaagat cttcaaattg aaaacgaaga
     3301 atttaaaaca tgtggggcta atgtgtaaga gtctgagata taataaattg ttagcggttc
     3361 tttatcattt ctatctcacc ctattatgat gtgacattta ttttacaaac taatttgttt
     3421 tgaacctgaa aataactttt tacaactaaa ttgtcgaaaa acagtgtgta catgatataa
     3481 taaatttata aattgaaaag aattcaaaat aaaaaataaa atatagattg agcacgaagt
     3541 gatttgaaat aaggttgtga aagggaatga caaggtcagc attaaaacca tttaaaaata
     3601 aacgcgttat ggttactgga cgtatacaac gtgttttgtt taaaaattat ttagatagac
     3661 atagcacatt taagccgaat gtaaggatat tattaaaaga tgtatttgtt tcaggtgtat
     3721 caatagatca tttatggtta tatgagacaa ataaatacta tgcattggca atggaactta
     3781 ttcatcaacg agtaaaattt agtgcgaatg ttgtaccata ttacaaaata aatagaaata
     3841 ataatttatt cgtacaagat tatggaatta agcgtaaagg taggttaatt actgaagaag
     3901 cttacaatca aaacaatcag tatcaggata agatatatga aaaattaccg gatatagatt
     3961 ttagactcga agatttttat agtaaggaaa actaaacaac atatataaag caccactaat
     4021 cgtagttgat agttgatcaa ttacacatta gtggtgcttt attttgaatt taaatttgat
     4081 aaatgaataa gagtatacgc attcatttaa gattagatga cattgataag tatgagtaat
     4141 gtattaggta accattaaat tcgttgttat gcgattctga tacaagatta tgataaaata
     4201 gctttaatta agaattttag ccatcatata gtcatcgtaa tatttaccat cgataaataa
     4261 cttatctttt aaaacgcctt cgatttgaaa atcggcactt ttaaaaagct cgagggcagg
     4321 ttggttattg agtggtacat ttgcttcaat tcggtgtatt tgattgttta aacaccaagc
     4381 cataatggca tcaagaagtg cttggccaat tccacgatgt tgatataatt tctttacacc
     4441 taaatcaatt ttagcaacat gtttaatgcg ttgaaatggt gtcgtattaa caaaagcaaa
     4501 gccaacgagt tgttcatcac tttcagcaac aaagatgact ttatgtggag aagtgatata
     4561 ttcttctaat tgtttactag ccgatgtgac gctaggatca tattctcctg gtgtgtagaa
     4621 catatacgga gattcgtcgt atattttcgc taacattgaa atgaaatttt ctacatcttt
     4681 gatactaact ctacgtataa tatgggccat aaaaagcctc cagtattttg aaattttaaa
     4741 acatttgcta ctattataat atatatgtaa ctaaaaggtg gagtaatatg ttttttggtt
     4801 acagtttaaa ggagtatttt agatgaaacc taaagtttta ttagcaggtg gaacaggata
     4861 tattggtaag tatttaagtg aagtgattga aaatgatgct gaacttttta ctatatcaaa
     4921 atatccagac aataaaaaaa cagatgatgt tgaaatgact tggattcagt gtgatatatt
     4981 tcattacgaa caggttgttg cagcaatgaa tcaaatagat attgctgtat tctttatcga
     5041 cccaacaaag aattctgcca aaataacaca atcatcagca agagatttaa cattaatcgc
     5101 agcagataat tttggtcgag cagcggctat caatcaagta aaaaaagtaa tctacatacc
     5161 tgggagtcgt tatgataatg aaacaattga acgcctaggt gcatatggca cacctgtaga
     5221 aacaacaaat ttagttttta aacgttcttt agttaatgta gaattacaag tttcaaagta
     5281 tgatgatgtt agatcaacga tgaaggtagt tttaccaaag ggatggacat taaagaacgt
     5341 tgtaaaccat tttattgcat ggatgggtta cactaaagga acttttgtga aaacagaaaa
     5401 atcacatgat caatttaaga tatatattaa gaataaggtg cgaccgctcg cagtatttaa
     5461 aatagaagaa acagctgacg gaataataac tttaatttta ttgagtggaa gtttagtgaa
     5521 aaaatacaca gttaatcaag ggaagttaga atttagatta atcaaagagt cggcagtcgt
     5581 ttatatacat ctatacgatt atatccctcg attattttgg ccgatttatt actttataca
     5641 agcaccaatg caaaaaatga tgattcatgg ctttgaagtt gactgccgga ttaaagattt
     5701 tcaaagtcga ttaaaatcag gagaaaatat gaaatatact aaatgatatt gggtgatatg
     5761 gatgcaaata ctactagtag aagatgacaa tactttgttt caagaattga aaaaagaatt
     5821 agaacaatgg gattttaatg ttgctggtat tgaagatttt ggcaaagtaa tggatacatt
     5881 tgaaagtttt aatcctgaaa ttgttatatt ggatgttcaa ttacctaaat atgatgggtt
     5941 ttattggtgc agaaaaatga gagaagtttc caacgtacca atattatttt tatcatctcg
     6001 tgataatcca atggatcaag tgatgagtat ggaacttggc gcagatgatt atatgcaaaa
     6061 gcctttttat acaaatgtat taattgctaa attacaagcg atttatcgtc gtgtctatga
     6121 gtttacagct gaagaaaaac gtacattgac ttggcaagat gctgtcgttg atctatcaaa
     6181 agatagtata caaaaaggtg acgatacgat tttcctgtcc aaaacagaaa tgattatatt
     6241 agaaattctt attaccaaaa aaaatcaaat cgtttcgaga gatacaatta tcactgcatt
     6301 atgggatgat gaagcatttg ttagtgataa tacgttaaca gtaaatgtga atcgtttacg
     6361 aaaaaaatta tctgaaatta gtatggatag tgcaatcgaa acaaaagtag gaaaaggata
     6421 tatggctcat gaataatttg aaatgggtag cttatttttt gaaatctcgc atgaactgga
     6481 tattttggat attgttttta aacttgctta tgttaggcat tagtctaatc gattatgatt
     6541 ttccaataga cagtttattt tatattgttt ctttgaattt aagtttaaca atgatttttc
     6601 ttatattgac atattttaaa gaagtaaaat tatataagca ttttgacaaa gataaagaaa
     6661 tagaagaaat taaacataaa gatttagcgg aaacgccatt tcaacgtcat acagttgatt
     6721 atttatatcg tcaaatctca gcgcacaaag aaaaggttgt tgagcaacag ttacaattga
     6781 acatgcatga acaaaccatt acagaatttg tgcacgacat aaaaacacct gtgacagcca
     6841 tgaaattatt aattgatcaa gaaaaaaatc aagaaagaaa acaggcatta ctatatgaat
     6901 ggtctcgtat aaactcgatg ctggatacac agctgtatat tactagatta gaatctcaac
     6961 gtaaagatat gtattttgat tacgtgtcac ttaaacgcat ggtcattgac gaaatacaat
     7021 taacaagaca tattagtcag gttaaaggta ttggttttga tgttgacttt aaagtggatg
     7081 attatgttta tacagatata aaatggtgcc gtatgattat tagacagatt ttgtcaaacg
     7141 cattgaaata tagtgagaat tttaatattg aaattgggac agaattaaat gatcaacatg
     7201 tttcgttata tattaaagac tatggcagag gtattagtaa aaaagatatg ccgcgaatat
     7261 ttgaacgagg atttacgtca acggctaaca gaaatgaaac gacgtcttca ggtatgggtc
     7321 tatatttagt aaatagtgta aaggatcaat taggtattca cctgcaagtc acgtcgactg
     7381 ttggtaaggg gacaactgtc agattgattt tcccattaca aaatgaaatt gttgaacgca
     7441 tgtcggaagt gacaaatttg tcattttaaa catgcgtttt gttacttaga attgatacat
     7501 caatgcagct tcaacgttat aataagatag atgttagtca tatgttaaat tgaagataca
     7561 agtgccaaag cctaaaggaa atgaagttaa gataaattat aggagtgtta aagtggcaat
     7621 tttagaagta aaacaattaa caaaaatata tggaactaaa aaaatggcac aagaagtgtt
     7681 gcgagatatc aatatgtcta ttgaagaagg cgagtttatt gctattatgg gtccctctgg
     7741 atctgggaaa acgacattat taaatgtttt aagttcaatt gattatattt cacaaggttc
     7801 tattacatta aaaggaaaaa aattagaaaa gctttcaaac aaggaattat ctgatatacg
     7861 caagcatgat attgggttta tttttcaaga gtataattta ctgcatacat tgactgttaa
     7921 agaaaacata atgttaccac taacggttca gaagttagat aaagaacata tgttaaatcg
     7981 ttatgaaaaa gtagcagaag cattaaatat attggatatt agtgataaat acccttctga
     8041 attgtctggt ggacaaagac aacgaacatc tgctgcaaga gcgtttatta cattaccttc
     8101 tattatattt gctgacgaac caacaggtgc actggattct aaaagtactc aagatttatt
     8161 aaaacgatta acaagaatga atgaagcatt taagtctaca attattatgg taacgcatga
     8221 tcctgttgca gcaagttatg ccaatcgagt agtgatgcta aaagatggtc aaattttcac
     8281 tgaattatac caaggggatg acgataaaca tacctttttc aaagaaataa tacgtgtaca
     8341 aagtgtttta ggtggcgtta attatgacct ttaacgagat aatatttaaa aattttcgtc
     8401 aaaatttatc acattatgcc atctatcttt tttcattaat tacgagtgta gtattgtatt
     8461 ttagctttgt agcattaaaa tacgcgcata aactaaacat gacagagtca tatccaatta
     8521 ttaaggaagg ctcacaagtc ggaagctact ttctattttt catcataatt gcatttttgt
     8581 tatatgccaa tgtgttattt attaaacgac gaagttatga gcttgcatta tatcaaacat
     8641 taggtttatc t
//
LOCUS       DAHHYJ010000115         8626 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000115 DAHHYJ010000000
VERSION     DAHHYJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.115"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            152..1003
                     /locus_tag="NBK22_002040"
     CDS             152..1003
                     /locus_tag="NBK22_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="base excision DNA repair protein"
                     /protein_id="HCD2415221.1"
                     /translation="MDISLNFNHYIDLKKSIKNLCLMKKFEPYVTNENYDFIEMPLVV
                     EGNYVLLKIYQETDALIKLHYQEVLNSNRKSVIQESDIVRISRSILNEALDINSNIKY
                     KYDTFGLTIISDADNYTTLIRTILAQQVSIEQANKLFLLFINTYGEIFELNNKEYTFL
                     NWDKIFSRIYKEQKVLVGTTNNRKQAIITLAELHIKNGLRENVLQVIPSIKGIGAWTV
                     QMFMLFQFPHVAFENIPVADIGLHRAIEKNHDLPHKSIDKSNGESYFQNMKSIDVFKY
                     WYIYMVE"
     gene            1962..2471
                     /locus_tag="NBK22_002041"
     CDS             1962..2471
                     /locus_tag="NBK22_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCD2415222.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(2584..3774)
                     /locus_tag="NBK22_002042"
     CDS             complement(2584..3774)
                     /locus_tag="NBK22_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2415223.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(3752..4927)
                     /locus_tag="NBK22_002043"
     CDS             complement(3752..4927)
                     /locus_tag="NBK22_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCD2415224.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLIAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            5360..6844
                     /locus_tag="NBK22_002044"
     CDS             5360..6844
                     /locus_tag="NBK22_002044"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCD2415225.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVDQ"
     gene            6825..7085
                     /locus_tag="NBK22_002045"
     CDS             6825..7085
                     /locus_tag="NBK22_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCD2415226.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            7085..>8626
                     /locus_tag="NBK22_002046"
     CDS             7085..>8626
                     /locus_tag="NBK22_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCD2415227.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEI"
BASE COUNT         3150 a         1201 c         1533 g         2742 t
ORIGIN      
        1 ttatcgctat tgaatgaacg atgtaattac atttctgtga tgccatatta tgtgagtgaa
       61 aataatttta atttatcttt ttcagaatgt aaaataccta aatatattaa gaaagattgg
      121 gctataacaa caatggagaa taaaaattaa catggatata tcattaaatt tcaatcatta
      181 tattgattta aaaaagagca ttaaaaattt atgcttaatg aaaaagtttg agccatatgt
      241 aaccaatgaa aattatgatt ttattgaaat gcctctggta gtagaaggaa attatgtgct
      301 cttaaaaatt tatcaagaaa cagacgcttt aattaaactt cattatcagg aagtattaaa
      361 ctctaatcga aaaagtgtaa tacaagaaag tgatatagtt agaatttcgc gaagcatact
      421 taatgaagct cttgatatca atagtaatat aaaatataaa tatgatactt ttggattaac
      481 tattatatcc gatgcagata attacacaac attaatacgg actatacttg ctcagcaagt
      541 gagcatagaa caagccaata aactattttt attatttatt aatacttatg gtgaaatttt
      601 tgaactaaac aataaagaat acactttttt aaattgggat aaaatattca gtcgaatata
      661 taaagaacag aaggttttag taggcacgac taataataga aagcaggcta tcataactct
      721 tgctgaatta catataaaaa acggcttaag agaaaatgtt ttacaagtta ttccaagtat
      781 aaaaggtata ggtgcttgga cagtgcaaat gtttatgcta tttcaatttc ctcacgtagc
      841 ttttgaaaat attcctgtag cagacattgg tttgcacaga gccatagaaa aaaatcatga
      901 tcttccacat aaatcgattg ataaaagtaa tggagaatct tactttcaaa acatgaaatc
      961 tatagatgtg tttaaatatt ggtatatata catggttgaa tagatagttt gaatcgtcta
     1021 ctcttaattg gacaaattct atgaggatag atattgtgat taaagaaaat aggcgatttt
     1081 atttggcagg agaaagaaga acatttaatt cagagtttaa gttataaatg gttagattat
     1141 atgaaatttg gaaacaaagg aatgaaatag tatgcgagta tgatttaaca ccttccgcgt
     1201 tagggaaatg gataaagcat catcaaaaca caggttgatt caatcatcaa gatagcttaa
     1261 cagatgaaga aaaagagctg attaaattac tcaaagaagt ttaatattta aaaatggaga
     1321 acgatatttt aaagttagca gcgttgatca tgggatgaaa atagaaatca ttcaaaagaa
     1381 tgcgcatcaa tattcagtat cagtattgtg taaaatctta aaaataccaa ggagaactta
     1441 ttatgaatta ataataagga ataatcagac ctaaaaacat ggtgaatcag aaatataatt
     1501 ataggtacgt ttaattttta atataaaaaa atggtaaaat acgaattaaa aacaacataa
     1561 atgacgaagg tttcactgta tcaagacgaa agattgattg tatcatgata aaacatcatc
     1621 tagtttctgt ttatacgaaa tttaaataca aaaattatta aaaatcattt aaaccgcact
     1681 tttaatagaa atcaaccaat gtagtattga atgatttaac atatgtaaaa gtcacaggaa
     1741 catgggatta catatgtttg tttttaggtt tatttaggct gtctagataa atttgacgat
     1801 gatatagttt ggtctataat ataatttata aaagatttta ctgtttgatt taatttaaat
     1861 ttgtcgaaat tgcaaaagat gtataatgaa ttatttttaa tgtaacggtt ttcaaagaaa
     1921 tttgatataa tagcaatagg ttaaacaaag gaggaattca gatgatttta ggattagcat
     1981 taattccatc aaagtcattt caagaagcgg tggattctta ccgtaaaaga tatgataaac
     2041 agtattcacg aattaaacca catgtgacaa ttaaagcgcc atttgaaatt gaagatggtg
     2101 atttagattc tgtcattgaa caggttagag ctcgtattaa tggtatacca gcagtagaag
     2161 ttcatgctac aaaagcttct agcttcaaac caacgaacaa tgtgatttac tttaaagttg
     2221 cgaagacgga cgacttagaa gaattgttta atcgctttaa tggagaagat ttctatggag
     2281 aagctgaaca tgtttttgtg ccacacttta caatagcaca aggactatct agccaagaat
     2341 tcgaagatat ttttggtcaa gtagcattag ctggagtaga ccataaagaa attatcgatg
     2401 aattaacttt gttacgtttt gacgatgacg aagataaatg gaaagttatt gaaacgttta
     2461 aattagctta agtaacataa tagtattgtt aatcgtagta tgtttgaatt aataagaaaa
     2521 tggtcatttt tattgaatgt aataaaaatg accattttct ttattttaaa atacgtttta
     2581 accttactta gctttttctc tatttgctat aaagtagctt ccataaaata cagctaagac
     2641 taaaaagatt aatgccgaga aataaaatgt attgtttaaa ttgttggtaa attgtgtaat
     2701 taatccgcca aataatggcc ctatcattga gccgaatcct tggatactat taaaaacacc
     2761 ccaagtttct tcttgttcat ctgatttgat aaatcgtgcc ataaaggtat tccatgctgg
     2821 taataagatg ccatacatta gaccgatagc taaagcgata atccacacga tgtgaatatt
     2881 aacaatcata gatagagtaa aaattaatat catgtataaa ataaatccgc ttagaataac
     2941 accatacata aagtttctgc tgcggttatc tattagtttc gataaaaata gcatcgaaac
     3001 tgcacagccg ataccaccaa taatgattgc aacagtatat tcaattgtgc ttacgttaat
     3061 aaccttagta gcatatgttg gtaatatagg aactagggca gcaattgcgg caccttgtaa
     3121 aagaatacca gggaacaaca ataaatggcg ctttgtcaca tcaacaattt gtctcaattg
     3181 agctttaact ggacgagtat tataatttgt taactttaca tcgacaaaat aatataatat
     3241 ccatgcaatt aaaacgacta aagacatcat gaaggcaaag cgtgttgggt gcactttgat
     3301 aagtagattc ataaaaacca tacctaccaa taggcctaac aaccatgaaa aataaacata
     3361 gcccatttgt ttgccacgtt tatcttcttc aacactggat aacataatga cccaaatagg
     3421 actaactgca ataccgagca tcatagcact aaatatgatt acaaaaggtg atgctggaaa
     3481 ccaaataact aaaaataaac ttgtaaatgc taaaataaat ccagtcgtta aaacgatttt
     3541 tgtgccgaat tttttcagta aaaatcctat aacaaagttt gtagatgcat cagcaataaa
     3601 atgtattgaa aatgctagag acgttattgc tacagcaatg gatgtaactg ttggcaagaa
     3661 attaatatag cttaggatat acatgcctct cgcaaattcc attaaaaata agataataag
     3721 cattaaaatg aaatttttat gattagcgta attatttaac gaagaatctt gcatataaag
     3781 gaacctttcc ataaatctct tgtggttgtg atgaatgacc gattaaatca agtaagtctc
     3841 gacatattgt ctgtgtagca tacttaattt tatcttgttc cattgtacta atcatgttag
     3901 ttaattgctc attaccgttc gttaaacttg ctacaatttt tattgcttct tctggagtat
     3961 cagcgatttt accaaaacct ttttcttcaa agtaaagggc attttcaagc tcttgaccag
     4021 gtgcaggatt taggaaaatc attggaatac aacgggcgaa accttcagtt attgtgatac
     4081 caccaggttt cgtaatcata agttgacttg atgccatcca ttcattcatg tgtttggtat
     4141 aacctagaat caatacattc tcgttagatt taaacttagc tattaaagaa cgctttagct
     4201 ctttgctctt accacaaatc ataactactt gtgcatttgc acttttcgct aatatatcag
     4261 taatcatcgt gtcaaaacct ttagatacac caaatgcacc agctgacatt aaaatagttt
     4321 gcttatctgg atctaagttg ttgtctatta accactgctt ttgattaata ggcgtttcaa
     4381 atttgttatc aataggaata cctgtcactt taactgttga aggatcaata cctacgtcta
     4441 tgaagtcttg tttcgtttct tttgttgcca cataatatct tgttgaatac ggagtaatcc
     4501 agtttttatg taagcgatag tctgtcatca ctgtagcaac tggaatatta atgttaaatt
     4561 gctcagttag taccgacata actggtgtag gaaacgttaa taatattaaa tctggctttt
     4621 cttttatcaa taaattaatt aacttattaa gtccatagta tttgtaaaaa catttgtcta
     4681 gtttatctgg gcggctgtaa taaaaccctt tgtacatatt tctaaaatat ttaaagctat
     4741 tgatatacca ttttttacaa atagaagtca aaattggatg agcttccata aataaatcgt
     4801 gctcaatgac gcttaaatgg tctagattca tatcattaag ttgattaacg atactctgtg
     4861 taacttgcat atgaccgtta ccgaatgagc cagtaataat caatatcttt ttattttgag
     4921 taaccattaa tagccaccct ccgttagttt gaaaatttta tttaagtgta acttatttta
     4981 cggcattata aaagaaataa agacgcaaag tcgttacatt tatagcaatt ttaatcaata
     5041 gatgaattga tacaaaataa aacgttattt tataaagcaa tttattgttc tatgttttat
     5101 ttgtatattt aaaattatcc agtatacaat tatagcatat ttttggaaac aattatgata
     5161 ttataccatg ttacaagatg gttttaataa tttaagatga gccataattg taaaactaat
     5221 tcataatacc gtatgtttta tttttaatag tagaaattag aaaatgctga ttagtaggat
     5281 ataacagtga aattataaat ttattaacat caacaaaacg tgtataataa acatattgta
     5341 gaaaaaggag cggttcagtt tggatgcaag tacgttgttt aagaaagtaa aagtaaagcg
     5401 tgtattgggt tctttagaac aacaaataga tgatatcact actgattcac gtacagcgag
     5461 agaaggtagc atttttgtcg cttcagttgg atatactgta gacagtcata agttctgtca
     5521 aaatgtagct gatcaagggt gtaagttggt agtggtcaat aaagaacaat cattaccagc
     5581 taacgtaaca caagtggttg tgccggacac attaagagta gctagtattc tagcacacac
     5641 attatatgat tatccgagtc atcagttagt gacatttggt gtaacgggta caaatggtaa
     5701 aacttctatt gcgacgatga ttcatttaat tcaaagaaag ttacaaaaaa atagtgcata
     5761 tttaggaact aatggtttcc aaattaatga aacaaagaca aaaggtgcaa atacgacacc
     5821 agaaacagtt tctttaacta agaaaattaa agaagcagtt gatgcaggcg ctgaatctat
     5881 gacattagaa gtatcaagcc atggcttagt attaggacga ctgcgaggcg ttgaatttga
     5941 cgttgcaata ttttcaaatt taacacaaga ccatttagat tttcatggca caatggaagc
     6001 atacggacac gcgaagtctt tattgtttag tcaattaggt gaagatttgt cgaaagaaaa
     6061 gtatgtcgtg ttaaacaatg acgattcatt ttctgagtat ttaagaacag tgacgcctta
     6121 tgaagtattt agttatggaa ttgatgagga agcccaattt atggctaaaa atattcaaga
     6181 atctttacaa ggtgtcagct ttgattttgt aacgcctttt ggaacttacc cagtaaaatc
     6241 gccttatgtt ggtaagttta atatttctaa tattatggcg gcaatgattg cggtgtggag
     6301 taaaggtaca tctttagaaa cgattattaa agctgttgaa aatttagaac ctgttgaagg
     6361 gcgattagaa gttttagatc cttcgttacc tattgattta attatcgatt atgcacatac
     6421 agctgatggt atgaacaaat taatcgatgc agtacagcct tttgtaaagc aaaagttgat
     6481 atttttagtt ggtatggcag gcgaacgtga tttaactaaa acgcctgaaa tggggcgagt
     6541 tgcctgtcgt gcagattatg tcattttcac accggataat ccggcaaatg atgacccgaa
     6601 aatgttaacg gcagaattag ccaaaggtgc aacacatcaa aactatattg aatttgatga
     6661 tcgtgcagaa gggataaaac atgcaattga catagctgag cctggggata ctgtcgtttt
     6721 agcatcaaaa ggaagagaac catatcaaat catgccaggg catattaagg tgccacatcg
     6781 agatgattta attggccttg aagcagctta caaaaagttc ggtggtggcc ctgttgatca
     6841 ataaaagatt tattgatgaa ggtaaaacta ttgatgttta tttattcgaa gcattaaata
     6901 accagataat cattgctata ccagattggt tttggtcata tcagatggca atgacattag
     6961 atgaagaaac ttgttttgaa gcaatactca tgcaattgtt tgtttttaaa gaagaggaag
     7021 aggcagaatc gattgcatca caactaacag attggataga aacatataaa aaggagaaag
     7081 actaatgaac ttaaagcaag aagttgagtc tagaaagact tttgcgatta tttcacatcc
     7141 cgatgcaggg aaaacaacgt taactgaaaa actattgtac ttcagtggtg ctattcgtga
     7201 agcgggtaca gttaaaggga agaagactgg taaatttgcg acaagtgact ggatgaaagt
     7261 tgaacaagag cgtggtattt ctgtaactag ttcagtaatg caatttgatt acgatgatta
     7321 taaaatcaat atcttagata caccaggaca tgaagacttt tcagaagata cgtatagaac
     7381 attaatggca gttgacagtg ctgtcatggt catagactgt gcaaaaggta ttgaaccaca
     7441 aacattgaag ttatttaaag tttgtaaaat gcgtggtatt ccaatcttta cattcattaa
     7501 taaattagac cgagtaggta aagaaccatt tgaattatta gatgaaatcg aagagacatt
     7561 aaatattgaa acatacccta tgaattggcc aattggtatg ggacaaagtt tctttggcat
     7621 cattgataga aagtctaaaa caattgaacc atttagagat gaagaaaata tattacattt
     7681 gaatgatgat tttgagttgg aagaagatca tgcaattaca aatgatagtg cttttgaaca
     7741 agcgattgaa gaattaatgt tggttgaaga agcgggtgaa gcctttgata atgacgcgct
     7801 gttgagtgga gacttaacac ctgtattttt cggttcagct ttagctaact ttggtgtaca
     7861 aaatttctta aatgcatatg ttgattttgc gccaatgcca aatgcgagac aaacaaaaga
     7921 agacgttgaa gtaagcccgt ttgatgattc attttcagga tttatcttta aaattcaagc
     7981 caacatggac cctaaacacc gtgatagaat tgcctttatg cgtgtcgtta gtggtgcatt
     8041 tgaacgtggt atggatgtta ctttgcaacg tactaataaa aagcaaaaga tcacacgttc
     8101 aacgtcattt atggcagacg ataaagaaac tgtgaatcat gctgtagcag gcgatatcat
     8161 tggactatat gatactggta attatcaaat tggagatact ttagttggtg gaaaacaaac
     8221 ctacagtttc caagatttac cacaatttac gccagaaatt tttatgaaag tttctgctaa
     8281 aaacgtcatg aaacagaagc atttccataa aggtattgaa caattagtac aagaaggtgc
     8341 gattcaatac tataaaacat tacacacaaa ccaaattatt ttaggtgctg ttggtcagtt
     8401 acaatttgaa gttttcgaac atagaatgaa aaacgaatat aatgttgatg ttgttatgga
     8461 gccagtaggc cgtaaaattg cacgttggat tgaaaatgaa gaccaaatta cagataagat
     8521 gaacacatca agatcgattt tagtgaaaga tagatatgac gatttagtat tcttatttga
     8581 aaatgaattt gcaacaagat ggtttgaaga gaaattccct gaaatt
//
LOCUS       DAHHYJ010000116         8600 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000116 DAHHYJ010000000
VERSION     DAHHYJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.116"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            140..1432
                     /gene="obgE"
                     /locus_tag="NBK22_002047"
     CDS             140..1432
                     /gene="obgE"
                     /locus_tag="NBK22_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCD2415228.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKVGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            1445..1900
                     /locus_tag="NBK22_002048"
     CDS             1445..1900
                     /locus_tag="NBK22_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCD2415229.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETSVEDVIGALRNLDYVSKVELISMSM"
     gene            1914..2516
                     /gene="ruvA"
                     /locus_tag="NBK22_002049"
     CDS             1914..2516
                     /gene="ruvA"
                     /locus_tag="NBK22_002049"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCD2415230.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEV
                     LIRTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            2548..3552
                     /gene="ruvB"
                     /locus_tag="NBK22_002050"
     CDS             2548..3552
                     /gene="ruvB"
                     /locus_tag="NBK22_002050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCD2415231.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERG
                     "
     gene            3554..4579
                     /gene="queA"
                     /locus_tag="NBK22_002051"
     CDS             3554..4579
                     /gene="queA"
                     /locus_tag="NBK22_002051"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCD2415232.1"
                     /translation="MNIEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTKSKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            4602..5741
                     /gene="tgt"
                     /locus_tag="NBK22_002052"
     CDS             4602..5741
                     /gene="tgt"
                     /locus_tag="NBK22_002052"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCD2415233.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            5760..6020
                     /gene="yajC"
                     /locus_tag="NBK22_002053"
     CDS             5760..6020
                     /gene="yajC"
                     /locus_tag="NBK22_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCD2415234.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            6294..8573
                     /gene="secDF"
                     /locus_tag="NBK22_002054"
     CDS             6294..8573
                     /gene="secDF"
                     /locus_tag="NBK22_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HCD2415235.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLAGVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIFKNQFWLFGVKKNKRHDINEGVDVHDLKTSFEK
                     WNFVKLAKPLIGVSILIVVVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQV
                     VKDSGLKADQIQINGKDNKVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPII
                     GQELAKNAMLALIYASIGIIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVD
                     LTFIAAVLTIVGYSINDTIVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSIN
                     TVLTVIVVVVAILFFGAPTIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSP
                     KHKLVVYKEKKSNDEKILV"
BASE COUNT         3028 a         1190 c         1716 g         2666 t
ORIGIN      
        1 tcggaaatat cagcaatttg agtaaagtaa atttaaaagc aactgcataa gagcccctaa
       61 ttatttacaa tatataaggg gctctaatat gctataatta ttgggaaaat gaaaattata
      121 tgtaaaagag gtgagatata tgtttgtcga tcaagtcaaa atatctctta aagccggtga
      181 tggtggtaat ggtattaccg catatagaag agaaaaatat gtaccatttg gtggaccagc
      241 tggcggtgac ggtggtaaag gtgcttcagt cgtatttgaa gtggatgaag gtttaagaac
      301 gttattagat tttagatatc aacgtcattt taaagcaagc aaaggtgaaa atggccaaag
      361 tagtaatatg catggtaaaa atgcggaaga tttagtatta aaagttccac ctggtacaat
      421 tattaaaaat gttgaaacag acgaagtgtt agcagatctt gttgaagatg gtcaaagagc
      481 tgtagtagcg aagggcggtc gaggtggccg aggtaattca cgttttgcaa cacctagaaa
      541 ccctgcacct gacttcagtg aaaaaggtga accaggtgag gaattagatg tatctttaga
      601 attgaaatta ttagctgatg taggattagt aggtttccct agtgtgggta aatcgacttt
      661 attatctatc gtttcaaaag ctaagcctaa agttggggca tatcatttta caacgattaa
      721 accaaatcta ggtgttgttt caacgcctga tcaacgtagt tttgttatgg cagatttacc
      781 aggtttaatt gaaggtgcat ctgatggcgt tggattagga catcaatttt taagacatgt
      841 agagagaaca aaagttattg ttcacatgat tgatatgagc ggttctgaag gtagagaacc
      901 tattgaagat tataaagtca ttaatcaaga attagctgcg tacgagcaac gtttagaaga
      961 tagacctcaa atcgtagtag ctaacaagat ggatttacct gaatcacaag ataatttaaa
     1021 cttgtttaaa gaagaaattg gcgaagatgt gccagttatt ccagtttcaa caataacgcg
     1081 tgataatatt gatcaattat tatatgcaat agcagataaa ttagaagaat ataaagatgt
     1141 tgacttcaca gttgaagaag aggagtcagt tggcattaac cgagtattat ataaacatac
     1201 accgtcacaa gataaattta caatttcaag agatgatgat ggtgcttatg tggtaagtgg
     1261 taatgctatt gaaagaatgt ttaaaatgac tgactttaac agtgatccag cagtacgtcg
     1321 atttgctcgt caaatgcgtt cgatgggtat tgatgatgca cttagagaac gtggttgtaa
     1381 aaatggtgat atcgttagaa ttcttggcgg agaatttgaa ttcgttgaat aggagcgtaa
     1441 catgatggac aataaagatt ataaaaagtt ttatttaatt agagaagatg tcttgcctga
     1501 atccgtggtt aaaacattga agattaaaga tgccttaaaa agtgatccga cattgtccat
     1561 ttatgatgcc gttaaacagt ttgatctatc tagaagtgct ttttataaat atagagaaac
     1621 gatatttcca gtagacgata aaatgcttga ccatcgagaa tttacattaa ttttatatgt
     1681 aactgatatt gtgggtatgt tggcacgtgt actagatgtt atatcaaagt tagaactatc
     1741 tgtattaacg attcatcaaa gtattccaat ggaagaaaaa gcaacaataa cattatcact
     1801 gaatgctaaa tctaaagaaa cttcagtaga agatgttatt ggtgctttga gaaatttaga
     1861 ttatgtatca aaagtagaat taattagtat gagtatgtaa ggatgtgcct ataatgtacg
     1921 cgtatgtcaa aggtaagtta acacatttat atcctacaca cgtagttgtt gaaactgctg
     1981 gtgttggtta tgaaattcaa acaccaaatt cttatcgttt tcaaaagcat ctagatcatg
     2041 aagttttaat tcgtacatct ttaattgttc gtgaagatgc acaattattg tatggattta
     2101 gtagtgaaga agagaaagat atgttcttga gtttaattaa agttactggt attggtccga
     2161 aatcagcttt agctatttta gcgacaagta cgcctaatga agtaaaacgt gccattgaaa
     2221 atgaaaatga tacgtattta actaaattcc caggaattgg taagaaaacg gcaagacaga
     2281 ttgtcttaga tttaaaaggt aaagtgaaaa tcactgaaga agatagcgat tcattattac
     2341 aagtagacgc tacttcgacg gtgcaagatc aattcgtgca agaagcaatg ttagcgttag
     2401 aagcattagg ttattctaaa cgagagcttg caaaagttga gaaaacgtta aataaaaata
     2461 aatatgactc agttgatgaa gctgttaagg caggtcttca attagttgta tcttaatttt
     2521 aaatagatta ataggggaag tgttgtcatg aatgagcgta tggttgatca atcaatgcat
     2581 agtgaagaaa ctgatttcga attgtcgctt agacctacga gattacgaca atatattggt
     2641 caaaattcaa taaaaagtaa tttagaagta tttattaaag cggctaaact tcgtcatgaa
     2701 ccattagatc atgtattgct ttttggcccc cctggattag gtaagacaac attatctaat
     2761 atcattgcca atgaaatgga agttaatata cgtacagtat cagggccttc attagaaaga
     2821 cctggtgatt tggctgcaat tttatcagga cttcaacctg gagatgtatt gtttattgat
     2881 gaaatacaca gactgagtag tgttgttgaa gaagtgttat accctgcaat ggaagatttc
     2941 tttttagata ttatcattgg taaaggcgat gaggctagaa gtatccgtat cgacttacct
     3001 ccattcactt tggtaggtgc aacaacgcga gctggcagct taacaggtcc actaagggat
     3061 cgatttggtg tgcacttaag attagaatat tataatgaat cagatttaaa agaaatcatt
     3121 attagaacag ctgaggtttt aggcacaggt attgatgaag aaagtgccat tgaacttgct
     3181 aaacgttcta gagggactcc aagagtagca aatcgactat tgaagcgggt aagagacttc
     3241 cagcaagtga atgaagatga acaaatatac attgaaacaa cgaagcacgc attaggttta
     3301 cttcaagttg atcaacacgg actagattac attgatcata aaatgatgaa ctgtattatt
     3361 aagcagtata atggtggacc tgttggttta gatacgattg ccgtaacaat tggtgaagaa
     3421 cgtattacaa ttgaggacgt ttatgagcca tttcttattc agaaaggctt tttagaacgt
     3481 acgccacgtg gcagaaaagc aacaccatta gcttatgaac attttgcaaa gtcgaatgag
     3541 gagagaggat aacgtgaata ttgaagaatt tgactatgac ttaccagaat cattaattgc
     3601 tcaaacgcct ttaaaagatc gtgatcatag tcgtttatta gtcatggata gagaaactgg
     3661 tgaaatgaaa catttacatt tcaaagatat cattgagtat tttagacctg gtgatacatt
     3721 agtgcttaac gatacgcgag taatgccagc tagacttttt ggtttaaaag aagaaactgg
     3781 tgcaaaagtt gaaatgttaa tgttaactca aattgaaggt aatgattggg aagtcttact
     3841 gaaaccagct aagcgtatta aagttggtaa taaattgaat tttggtaatg gcaaaattat
     3901 agctgagtgc ataaaagaaa tggatcaagg tggacgcatc atgcgtttac attatgaagg
     3961 tattttacaa gaaagattag atgaattagg ggaaatgcca ctgccaccat acatcaaaga
     4021 acgtttagat gatccagatc gttatcaaac agtttacgct aaagaaagtg gttcagcggc
     4081 agcaccaaca gcaggattac attttactga tgagttatta actgaaatta aaaataaagg
     4141 cgttaatatc gcatttgtta cattacatgt tgggttaggt acgtttagac cggtgagcgt
     4201 cgatgatgtg aatgaccacg aaatgcatag tgaatattat caaatgacac aagaaacagc
     4261 tgatttatta aatgatacta agtccaaagg acatcgcatt atatcagttg gtacaacttc
     4321 aacacgtaca cttgaaacaa ttcgacgcga tcatgataaa tttgttgaaa cgagtggctg
     4381 gactaatata tttatttatc caggatttga ttttaaagca attgatggcc agattactaa
     4441 ttttcattta ccaaaatcaa cattagttat gctagtatca gcgtttagta ctcgtgaaaa
     4501 tgttctgaat gcttataaaa cagcagtaaa tttagaatat agattcttta gttttggcga
     4561 tgcaatgtta attatataaa aagaatgtga ggattttgaa tatgcctgca gtaacatacg
     4621 aacacattaa aacttgtaaa caatcaggtg cgcgtttagg tatcgtgcac acaccacacg
     4681 gttcatttga aacacctatg tttatgccag ttggtactaa agcaaccgtt aaaacaatga
     4741 gtccagaaga gttaagacaa attgaagcaa aaatcatttt gggcaacaca tatcatttgt
     4801 ggttacaacc cggaaatgat attatcaaac acgctggggg attacataaa ttcatgaatt
     4861 gggatggtcc gattcttaca gattcaggcg gtttccaagt gtttagttta agtaatttac
     4921 gtaaaattac agaagaaggc gtggaattta gacatcatac taatgggtct aaattatttt
     4981 tgagtcctga gaaatcaatg caaattcaaa atgatttagg atctgatatt atgatggcat
     5041 ttgatgaatg tccaccgatg cctgctgaat atgattatgt aaaaaaatct attgaacgta
     5101 caacacgttg ggcgaaaaga tgtctagatg cacaccaaag acctgaagat caagcattgt
     5161 tcggcattat acaaggtggc gaatatgaag atttaagaga acaaagtgca aaggatttag
     5221 tagaattaga ttttcctggt tatgcaatcg gcggtttgtc agttggtgaa cctaaaccgg
     5281 ttatgtataa aatggttgaa catacagagc agtttatgcc taaagataaa ccaagatatt
     5341 taatgggtgt aggatctcca gatgcgttaa tcgaatgtag tattcgcggc atggatatgt
     5401 ttgattgtgt cttaccgaca cgtattgcca gaaatggtac ttgtatgaca tcgcaaggtc
     5461 gtttagttat taaaaatgca aaatttgcag atgatttaag accgttagat gagaattgtg
     5521 actgttatac atgtcaaaac tattcaagag cgtatatacg tcatttaatc aaggcagagg
     5581 aaacttttgg tattcgtctt actactattc ataatttaca ttttctgcta aaattaatgg
     5641 aagatataag acaagccatt cgagaagatc gtcttttaga tttcaaagaa gaattcttcg
     5701 agcaatatgg attaaatgtt gagaacccaa aaaactttta agcaagagga gcgtataaaa
     5761 tgcaattttc attactaata tatatagtcg taatttttgc ggttatgtat ttcttgatga
     5821 tcagaccaca acaaaaacgt gcgaaacagc atcgtgagtt gattaataac attcaatctg
     5881 gtcaaagaat tacaactatt ggtggtatta aaggtactgt taaagcagta gatgaaacaa
     5941 ctgttgttat tacagttaat ggtcatggta ctgaattaac tttcgaaaaa cctgctatta
     6001 aacaagttga cccttcataa tgagaagtgg tattaaaaag gatgaaacga atagcgtttc
     6061 atccttttta agttataagt agtatgtaga atagcttatt tttattccaa aaaagaaatt
     6121 gtgatacata ataattgtta aatttaaatt aaatatatct atgactattt ttactccctg
     6181 ctctagtatg ttaatatgta taaggctaat aaattttgta agaaagattt aacttgcgtt
     6241 ataaaatagt attcaagtat tctaaataca tacgtaaata atgaggtgtt catgtgaaga
     6301 aaagtagtag aataattgcg ttcttgcttt tagtcgtatt gttgtttgcc ggaatggctg
     6361 caacttataa aagtgttata aagaatgtca atttaggact tgatttgcaa ggtggatttg
     6421 aggtgcttta tcaagtcgat cctttaaata aaggcgacaa aatagataaa aaagccttac
     6481 aatcaacagc gcaaacatta gaaaatcgtg ttaacgtttt aggtgtttcg gaacctaaaa
     6541 ttcaagttga agaacctaat agaattagag tacaacttgc tggtgtaaca gaccagaatg
     6601 aagcacgtaa aattttatct tctcaagcta atttaacaat tcgcgatgca gaagataaag
     6661 taaaattaag cggttctgat attaaacagg gatctgccaa acaagaattt aaacaggaaa
     6721 caaaccagcc tacagttact tttaaagtaa aagacaaaaa caaatttaaa aaagtaacgg
     6781 aagaaatctc taagaaacgt gataatgtca tggttgtttg gttagatttc aaaaaaggtg
     6841 acagctataa aaaagaagca caaaagaaaa accctaaatt tatttctgca gcaagtgttg
     6901 atcaaccaat caattctgat agcgtagaaa tctcgggtgg atttaaagga caagaaggcg
     6961 ttaaaaaagc aaaacaaatc gccgaattgt taaatgcagg ttctttacca gttgacttga
     7021 aagaaattta ttcaaactca gtaggtgccc aatttggtca agatgcatta gataaaactg
     7081 tgtttgcatc gtttattggt gtagcattaa tttatttatt catgcttgga ttctaccgat
     7141 tacctggttt agtagcgatt attgcattga caacttatat ctatctaacg ttagtagcat
     7201 ttaatttcat ttccggggtt ctaactttac caggattagc ggcgttggta ttaggtgtag
     7261 gtatggctgt agatgccaat attatcatgt atgagcgtat taaggatgaa cttcgaatag
     7321 gtagaacgat aaagcaagcc ttttctaaag caaacaaaag ttcattccta acaatttttg
     7381 attctaactt aacaacagtt atcgccgcag cagtattatt cttcttcggt gaaagttcag
     7441 ttaaaggttt cgcgacaatg ttattattag gtattctaat gatctttgtt acagccgtgt
     7501 tcttatcaag attcttatta tcattacttg tttcatcaaa tatattcaaa aatcaatttt
     7561 ggttatttgg tgttaaaaag aataaacgac atgatattaa tgaaggtgta gatgttcatg
     7621 accttaaaac ttcattcgag aaatggaatt ttgttaaatt agctaagcca ttaattggag
     7681 taagtatttt aattgtagtt gtcggtttag ttattcttta tatcttcaag ttaaacttag
     7741 gaattgattt ctcaagtggt acacgcgtag atttccaatc taaacaagca attactcaac
     7801 aaaaggtaga gcaagttgta aaagatagtg gacttaaagc ggatcaaatt cagatcaatg
     7861 gtaaagacaa taaagtagca actgttcaat ttaaagacga tttaacgcgt gctcaagata
     7921 ataaattgag tgataatatt aaatcgaaat tcggtgacac acctcaaatt aatactgtat
     7981 caccaattat tggacaggaa ttagcaaaaa atgcaatgct tgcattaatc tatgcatcga
     8041 taggtatcat catctatgta tcattacgat ttgaatggcg aatgggtctt tcatctgtat
     8101 tggcattatt acatgatgta ttcattatag tagcgatttt cagtttattt agaattgaag
     8161 tagatttaac atttatcgcc gctgtattaa caattgtcgg ttattcaatt aatgatacaa
     8221 tcgtaacgtt tgaccgtgta cgtgaaaact tacaaaaggt taaagtgatt acgacaacag
     8281 aacaaattga tgatatcgtt aatagatcaa ttagacagac aatgacacgt tcaattaata
     8341 cagtattaac agttattgta gtagtagttg ctatactatt cttcggtgct cctacgatat
     8401 tcaactttac tttagcatta tttatcggat tgatttctgg tgtattctct tcaatcttca
     8461 ttgccgttcc gctatgggga ataatgaaaa aacgtcagct gaaaaaatcg ccgaaacaca
     8521 aattagttgt atataaagaa aagaaatcga acgatgaaaa gattttagtt taaaatgaat
     8581 taagcggtat gtgaaacaat
//
LOCUS       DAHHYJ010000117         8416 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000117 DAHHYJ010000000
VERSION     DAHHYJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.117"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(188..1066)
                     /locus_tag="NBK22_002055"
     CDS             complement(188..1066)
                     /locus_tag="NBK22_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCD2415236.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMK
                     EETLNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLDNLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQEHLKSLDADNEDIPFSGAF
                     SIKFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            complement(1245..1625)
                     /gene="gcvH"
                     /locus_tag="NBK22_002056"
     CDS             complement(1245..1625)
                     /gene="gcvH"
                     /locus_tag="NBK22_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCD2415237.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQIEALLTAEKYSKMIGE"
     gene            complement(1783..2139)
                     /locus_tag="NBK22_002057"
     CDS             complement(1783..2139)
                     /locus_tag="NBK22_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCD2415238.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            2283..2603
                     /locus_tag="NBK22_002058"
     CDS             2283..2603
                     /locus_tag="NBK22_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD2415239.1"
                     /translation="MQSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            complement(2753..3292)
                     /locus_tag="NBK22_002059"
     CDS             complement(2753..3292)
                     /locus_tag="NBK22_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCD2415240.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(3375..4091)
                     /locus_tag="NBK22_002060"
     CDS             complement(3375..4091)
                     /locus_tag="NBK22_002060"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCD2415241.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMID
                     IEWQADIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EPQAPGQIDVTDLKAQVTLY"
     gene            4239..4661
                     /locus_tag="NBK22_002061"
     CDS             4239..4661
                     /locus_tag="NBK22_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCD2415242.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAGYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDSDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            5060..5554
                     /locus_tag="NBK22_002062"
     CDS             5060..5554
                     /locus_tag="NBK22_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2415243.1"
                     /translation="MIEIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            5708..6325
                     /locus_tag="NBK22_002063"
     CDS             5708..6325
                     /locus_tag="NBK22_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCD2415244.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGETQ
                     IMSPMKQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLLF"
     gene            complement(6398..6979)
                     /locus_tag="NBK22_002064"
     CDS             complement(6398..6979)
                     /locus_tag="NBK22_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD2415245.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGLFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEERIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            7203..7439
                     /locus_tag="NBK22_002065"
     CDS             7203..7439
                     /locus_tag="NBK22_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415246.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            complement(7436..7639)
                     /locus_tag="NBK22_002066"
     CDS             complement(7436..7639)
                     /locus_tag="NBK22_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD2415247.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            7980..8240
                     /locus_tag="NBK22_002067"
     CDS             7980..8240
                     /locus_tag="NBK22_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415248.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            8269..>8416
                     /locus_tag="NBK22_002068"
     CDS             8269..>8416
                     /locus_tag="NBK22_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415249.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFG"
BASE COUNT         2775 a         1391 c         1179 g         3071 t
ORIGIN      
        1 aattctacag gcaatgcaag ttggggaggg gccccaacaa agagaaattg gattctcaat
       61 ttctacagac aatgcaagtt ggggtgggac gacgaaataa attttgcgaa aatatcattt
      121 ctgtcccact ccctagattg atctatagat actacactta ttaaagtaat atatttttat
      181 gattctctta gctgcaatcc catgaataca tgtaatcatc aaacttcata gcctcaaggt
      241 cagtagattt tactaagata ttggcaatac ctaaatcacc ccatataatc gaagaattac
      301 catctcttgt agaatcaatt tgcagtaaga ctctatcata cattttcagt tcttgatcgt
      361 tcgttcttgg atctatttgg tcaaaatatg gataaccacc aagttgatta aatggttcct
      421 tagcagtaaa tgtgttacac acaggttcca tcaattcatc ataaccacca aacattgatt
      481 ttagcgcgaa ggatggattg actttattcc acaatgcaag cacgtcctca tcgtacttat
      541 aatcagtaca tgtaatagtt tgtttcgaca atctaaattt tatagaaaat gctccactga
      601 acgggatatc ctcattatca gcatctaatg acttcaaatg ctcttgaata ttttcgagtg
      661 ataaatctgt aattggctct tttatataaa caagccttga gtttatattg tttcccttta
      721 aattttcaga cataccatac atttgatctt ctgaactaat ccaaaactgc aatatccctt
      781 cttttacagg aaataattct tgttgttctg gaagattatc caaattaatc tgtgctaaca
      841 ataccattgg tacattattt tcatctaatg gtatatcttt aaaagatttt aaaaatggca
      901 ttccaccaat cttactctgg tttaatgttt cttctttcat ctcttttaga tccatttcaa
      961 tacatggtag tactggtaaa ttaccgtttt ctacagattc tacgaaacgg ttatatgctt
     1021 tttgctcctt tttagaaaga tgactaacgt cttgaatgtt tagcatatta acttccccca
     1081 atttttattt ttaaaatagc taaattcatt attttcatca ttcttgaata ataaaatgta
     1141 gcaatactcc aaataatagt acctaataaa ttttctttca tattatttac ttactcagac
     1201 taaaaataaa ctcctcaatc ttagattaag gagttacacg gtgattattc accaatcatt
     1261 tttgaatatt tttcagctgt taataaagct tcaatctgac tttcatcact aatttctact
     1321 tttaccatcc atgctttttc gtatggagat tcatttacaa attcgggact atcttctagt
     1381 tcttcgttga cttcaactac tttaccagag attggtgcat ataattctga tacagtttta
     1441 actgattcta cgctaccaaa cgtatcccct tcattaattt catcatctgt ttctggtaat
     1501 tcaacgaaaa caatatcacc taactcgctt tgtgcgtatt ctgtgattcc aattgttgct
     1561 acattacctt caactttaac ccattcatgc tcttttgaat atttcaattc atttggtact
     1621 gccaagagaa tcccctccta attaatcttt acatcattaa tgatgccata aggctagatg
     1681 ctattcaact atatttataa aaattaacaa ttgtatagct tatttcattt tacatgtaat
     1741 gaatctaaat atcgagatat ttagtaaacg tttacatttc acttacgcta accaagtctc
     1801 tttatattga tcttctttaa atcctaatgt tatcttatcg cccattactg ctagaggacg
     1861 ctttactaac ataccatcag atgataacaa ctctaacttt tcatcatctg ataaagtttg
     1921 taatttattt ttcaaatcaa gctcacgata tttcgcgccg tgtgtattaa acaatttatt
     1981 aatttctacg cctgtatttg caattattgt tttaaattca tttattgtag gtgtatgttg
     2041 aacgatatca attggttcat aacttacgcc atattcatct aaaaactttg ctgccttttt
     2101 acaagttgta caattcttat attggtaaaa tttaatcata cgactgcaac ctcctttatg
     2161 tatttaaaat aaatatatca ttttccatag caaaaggctt tcattttgaa gctaaaattt
     2221 tttattatga tataaattta gctataataa aaattattaa actatctaga ggagttgtta
     2281 caatgcaatc aatcaaaagt aatgaatcat ttaaatctgt aattaatagc gatacacctg
     2341 taattgttaa atttgaggca ggatggtgcc cagactgtcg tgctatggat ttatggattg
     2401 acccaatcgt agaacaatat aatgattacc aatggtatac tgttaatcgt gatgaattag
     2461 aagatgtagt tgttgaaaat gaagttatgg gtatccctag cttgctagta tttaaaaacg
     2521 gcgacaaaat tgcacacctt cattcagcaa atgctaagtc acctgagcaa gttgaatcat
     2581 ttttagcaga aacttttaaa taaatactaa aataaaaatg tgtatatact gcacaataaa
     2641 aagctgagac tgtgattgta ccgactctag taagttagat taaatctcta actcgataga
     2701 tgtccatcaa ttcatgactc agcttttatt aatgtttcta taattataca tattaatata
     2761 atgtaattaa gtttctattt ttacgcggtg ctttaggcat atcttcttct aaatccgtta
     2821 aatataagaa tccagcaaga acttcatctt tctttatacc aagtgttttt cttactttag
     2881 gatcatagat atataatggc gatttccaac atgttcctat tcccgcttca taaagtaaca
     2941 acataagatt ttgtgcatat gcaccaaatg caaagtagtt ttcatcattt tgacgttgtc
     3001 ttggatctgt ttttaaaata agcaataaca tgccacctag tttcgtaact gcatcataat
     3061 gacattgttg cttatctaat tcattaggaa atgcaaattt agaaatatcc ttactcatat
     3121 ctcctaatct gtctttcgga acatgcacaa ctctccatgg ttccctcatt ccgtgatttg
     3181 gagcatcagc agctttctca attgcttgat atagcaatgc gtcatctatg tgcatatctc
     3241 ttttaaattt tttcacactt cttctattag ctattgcttg ttgtaattcc atctaaaatc
     3301 gctcctccac gaacaatgat aatacttctc aattataaga cagcacttac acaaaagcaa
     3361 cctaattcat tttattagta taaagtcact tgtgctttta aatcagtaac atcaatctgt
     3421 cctggagctt gtggttctcc gatacaacca taagtcaatg caccaccaaa aacgccttga
     3481 gccgttctac ttattagtcc aagtttagac attgaaatac caacaacttt gcagtccata
     3541 gtatctgaaa atgtagacat tgcctgcaat aaatttaaca catcattttt attatgtggc
     3601 attactgcta atttaacgta ttctgggttg aatttttgca ttttaaaaaa tataaattgc
     3661 aattcatcta atggaggcgt actttcgaaa ttatgatgtg atataaccac ctctttatta
     3721 tactgttgca aatgtgtaat gattcgttga tgtttttcaa tgtcaatatc tgcttgccat
     3781 tctatatcaa tcatatctat gccattgata tttgctaagt ctgatattaa attaagatac
     3841 gagtcatttg taaattgccc atagccacct tgtaactttg tacgatacgt aactaataat
     3901 ttgaatgaat cttgcataac cttcagcttt gtaatcattt ctgccacttg atcaactgtg
     3961 acattttcaa tttgatcaat tcgtaattct aatacgtcta ttgcatcaat acgatgatta
     4021 attttttgaa ttaaagtttc ttcgatagat aattgtggcg cgatagtcgc tactacttcc
     4081 acatgtgtca tagtgaatcc ctcatttcag atactcatta tttttattgt aaacaacaac
     4141 tttttatcct tcaatcaaac aatgctataa atgaaaatga ttaaggtatt ttcgaatggg
     4201 taaagcataa atgtatttta aattaggagg ttataagtat ggcaatacat tatgaaacta
     4261 aagcgacgaa tgttggcgga cgtaaaggac atgtttatac tgatgatcga gcattagata
     4321 ttgatatcgt tccgcctgct caagcagatg gtaaagctac taaccccgaa caattatttg
     4381 cagcaggtta tgcatcttgc ttcaacggtg ctttcgacct aattttaaag caaaacaaag
     4441 tgcgtgatgc tcatccagaa gtaacactaa cagtgagact agaagatgat tcagactcag
     4501 aaagtcctaa attaagtgtt tcaattgatg cgacaattaa aaatgttata tctcaagaag
     4561 aagctgaaaa atatttacaa atggctcatg aattttgtcc atattcaaaa gcgactcaag
     4621 gaaatattaa tgtcgattta aatgtaaatg ttgtagatta gcattaactt aaagagatta
     4681 ttcaacgtta ttaataaaat tcacataaaa ttcaaatttg ccaaccaaaa atttttggtt
     4741 ggttattttt tctattcgtg actgaaattt ctggcaatat ttaactgaaa atgattgtct
     4801 cttagtcatc ataaatgtga ccggttccaa tactggcttg acttcttcgc ataacgtctc
     4861 caaataaaag tccagtgtcg atcaataacc aattccataa tttcctgaac ttaaaaataa
     4921 atataattaa attgataatc catttacaag aatgtgcata taaatcagtg attgcaaaat
     4981 tttaataaac attttaatat gtaattaaga aattttttta gtacaaaatc tttcttaata
     5041 atgattaagg aggtcagtaa tgattgaaat taaaacatta acgaataatg attttaatga
     5101 gtataagaga cttgtttcga cagtcaatga agaattcact caagattcac attatagtca
     5161 aacaatgact gacaccttaa tacatgacat tttaaatcaa ggttcaccga aatgtattgt
     5221 atttggctgt tatgaaaacg aaacacttat cgcaacagct gccttagaac aaattcgata
     5281 cgttggaaaa gaacataaat cattaattaa atacaacttt gttactaata acgataaatc
     5341 gattaatagc gagctcatta atttcattat taattatgca cggcagaaca attacgaatc
     5401 tttacttaca tcaattgtgt caaacaacat aggtgctaaa gttttctata gtgcactagg
     5461 attcgacatt cttggttttg agaaaaatgc aattaaaatc ggaaatacct atttcgatga
     5521 acattggctt ttttatgatt tgattaataa gtaatacagt tttatatatt ctacatttct
     5581 cataaattca aatcatatag gttctatttt tcatacaact actctactat tgagtagttg
     5641 ttttttattt gatatgataa caataagttt ttcagaatat tctatttttt aggggtgtca
     5701 aaacttaatg gtaaccgcaa ttattcatgg atttatctta gcgattggtc taattttacc
     5761 tttaggtgca caaaatgtat ttatttttaa ccaaggagct aatcaaccaa aatatagata
     5821 tgtattgcct gcaataatta cagccgggtt gtcagacagc ttacttatta ttattgcagt
     5881 ggtaggagta tctatcatta ttatgtcttt acctgtactt caagcaatta tttatatagt
     5941 tggtttaatt ttcttgatgt atatggcttg gaccatttgg catgataaac cctcaacaga
     6001 tggagaaact caaattatgt ctccaatgaa acaagtaagt tttgctttat cagtttcatt
     6061 actcaatcca catgctattt tagatacaat tggtgtaatt ggtagtagtg ctgcattata
     6121 tagtggcagc aataaaattg cgtttacaat cgcttgtatt agcgtttcat ggttatggtt
     6181 ctttttactc gcaattttag gaaaaatggt tggatcaatt gataaaaccg gaaaattatt
     6241 aacaataatc aataaaatat caagcataat aattattatt gttgctctga tgatattaca
     6301 aaaacttatt caattattat tctaaagcgt tatgtagttg tattttaaaa gcgttataca
     6361 aaaaacatac ttgtgtctat gtataacgct ttatttatta taaatttgga tcaattaact
     6421 caacaaattt aaatgttcca ttgtcatatt caaatttcag gatattacaa ttaccgatat
     6481 tatgctttaa tgcttcatct agtttaaggc aatatcttaa aaatactccc attattgtcc
     6541 catgactcac gactaatgca ttattctttg tttgagacat aatgtcatgt aaagttttca
     6601 caatgcgttc ttcaacttgt tgccttgcct ctcctttgaa aggaacaatt cgatctccga
     6661 ataagtcttc aggcttgtat agattatcaa atagatagac tgactcacct tcaaataatc
     6721 caaaatgcca ttccttcagt cccttaaaac gttgatacga ttggttaggt gcaacatttt
     6781 caagtgtgtc acttgcgcgt tcttgcgttg atgatgcata taaatcgaag tttatcccct
     6841 tagtttcata ataactacgt gccttttgag cttgagcaat tccaagttct gttagcggcg
     6901 aatctccaaa tccctgaatt agtcccttaa aattaaacaa agtttgtccg tggcgcatta
     6961 aatataacgt tttagccaac aataacacct cttcaataaa aagtattgat agctcaaaat
     7021 gtattctctt tttattaaaa tgagtaccaa atatattaaa ccattattta aactttttcg
     7081 gtagcgaaaa cctttgcttt tgatgtacat tttttcataa gtaatgatga ttttagttta
     7141 aaagataatg tgacggggta aaacgcaatg aagagtacag aattttaaag gagtgagtgt
     7201 ttatggataa tcataatcaa tgtaattatg tgaatccaca aaatgtttca cttgactggg
     7261 aatgttttat aattagtaaa agtgaaatgt tgttagatgg tgtaccaaat gaacttatca
     7321 acacttggtt agataaagac attattacac cattttcgat aagaaatgat gaaataaact
     7381 ttaaaacaaa agatatttgg gatgcactaa tacatcataa ttggtactac tcaaattaat
     7441 ttgccatata taagctaata gctctatttg tagtttcaat taattgatga tctgtgtaat
     7501 tagacatcac atatgctcta attacttggt gtctttcttc taatcgaggg aatgctttat
     7561 cttcactaac gtgttcggcc aatataccaa atggtgtgtt atcattttga aaacccatga
     7621 tgaaattgta aaacgtcata agacttgccc ccttcattta ttaagcgcta tttaaagtgt
     7681 tattattaat cttatcagaa aactagtatt tgtaaatgtg caaaagtgat tttgcacatt
     7741 tttttgatat ctgattctaa aaacattaac gaattataca actttaaaag tttttagtat
     7801 gtaaatattc ttttcatatt aatacaatac acccctatat atgctcactt gtttgaatta
     7861 ataaacttta gcattctgca aataatattt taaaaataac tatacttttt atttacctat
     7921 tgaaaaatag tgttatcata tttattaaga agctattttt atactagtag gtgtcagtca
     7981 tgaataacat tttgttaaat gctatcaata tagttattac taccactttt gttatcttta
     8041 atattttaat cacatataat aaagatttag atgatttatg ttggctcctg cctggtatta
     8101 tcatttgtgg tgtgatactc atcgtatcct ttaccattgc aatgataact aaaaactggt
     8161 taagtgaaat attatttttt ataaatatcg tactcgttct ctattacatt tatcctattt
     8221 tttatagttt tataggttaa taattacggt atataaggag ggtttcacat gtctttacat
     8281 tttgcaattc tgttttggct agcattaatt ttcttagttg ccgctacgtt tatactcgta
     8341 ttaatgaaaa aaactggcaa agaatctaaa aaagagtcct atttaagttt cactgtcatt
     8401 ctctatattt ttggat
//
LOCUS       DAHHYJ010000118         8217 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000118 DAHHYJ010000000
VERSION     DAHHYJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.118"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(66..392)
                     /locus_tag="NBK22_002069"
     CDS             complement(66..392)
                     /locus_tag="NBK22_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD2415250.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
     gene            complement(456..1199)
                     /locus_tag="NBK22_002070"
     CDS             complement(456..1199)
                     /locus_tag="NBK22_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCD2415251.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDRLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELFRNLKCYLNMCQLKLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            complement(1225..3180)
                     /locus_tag="NBK22_002071"
     CDS             complement(1225..3180)
                     /locus_tag="NBK22_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2415252.1"
                     /translation="MTDNYSLHIFKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQSYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEHCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGNTPKLIRKRYSPTSHSINHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLGVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIAFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPKELNTRYDKEIDHLIDY
                     FILPLRQIEQVSIACSKIICDVDLINENNDLKEKITISDFNKVTELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKGLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
     gene            3790..4590
                     /locus_tag="NBK22_002072"
     CDS             3790..4590
                     /locus_tag="NBK22_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD2415253.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYTS
                     GRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQG
                     AYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
     gene            complement(4608..4850)
                     /locus_tag="NBK22_002073"
     CDS             complement(4608..4850)
                     /locus_tag="NBK22_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415254.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDMPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(5024..6424)
                     /gene="nhaC"
                     /locus_tag="NBK22_002074"
     CDS             complement(5024..6424)
                     /gene="nhaC"
                     /locus_tag="NBK22_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCD2415255.1"
                     /translation="MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            complement(6519..7601)
                     /locus_tag="NBK22_002075"
     CDS             complement(6519..7601)
                     /locus_tag="NBK22_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCD2415256.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFNDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVEEIGLDKLDLEKYLK"
     gene            7849..>8217
                     /locus_tag="NBK22_002076"
     CDS             7849..>8217
                     /locus_tag="NBK22_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCD2415257.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIVLVVITLIGVFLIATDISFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACV"
BASE COUNT         2796 a         1482 c         1045 g         2894 t
ORIGIN      
        1 ccatttatct ttaaatgcaa tgagttatca ttcaaactct tcttatcaac ctacatacat
       61 tgacattata ctatcatatg ttgttttaca tattcaacaa acatttcaac gttatcactt
      121 ttaatatcat gcattgggac attaccaata ttaaatttga acattatagt ttgaccatca
      181 taatctatct tctctatttc attataacta atacttctat aataaaattg gccattcata
      241 tctacattca taattaatct ttcatttgtt gctataaatg ccccttcaaa ttcattctca
      301 ccttgaactt gatactcaat tattcctagc actgacggac cctttttttc agttggaaat
      361 aaatcattcg gattcacatt atctaaaatc ataatatccc tcccacttaa aactataaac
      421 tattcttcat aggtatatga aatacaagtg attaactatt tattattaag cttaacttgt
      481 attccctttt caagataatt ataacatgct tgaataagca tagtgacttt agaaaacttt
      541 tctcgcttga ttgttacaat aacacgtctt tcatctaatt cacatctctc ttttttaact
      601 aatcccttca aaattaaaca ctttaaaatc gggctaaccg caaatatcgg gtgatggaat
      661 tcaccctgta attctttaac cgtcaattga cctttatgca gatttaatat tcctaaaacg
      721 tataactctt ctaattttaa ttgacacata tttaaataac actttagatt tcgaaataat
      781 tcttgtgacg acaataaata cgatacatag tcacaactaa aatcaaatgt atattgacca
      841 aaataacgtt caatatagtc actaagctca ttattcatat attctatttt atctatatgc
      901 attttattaa tcgaaatagt caatttacgt tgatcccttt gatctcttcc tttcaatagc
      961 cattgatgat tgtttaaata acttaacatt tgaagaagtg ccatcttaga ttggatttct
     1021 ttcattttta ataacctatc tattgaagcg ctattggaat cagaattatc atatttaact
     1081 aatagattta atagtttcac ttgagtcaaa gtgagatgaa atgaatattt caacatatca
     1141 ttaatgactt tcaacattag tatgtgtgca gttaaacgct taactttaaa tttagacatc
     1201 tttaaaacct ctcttaaacc atgcctatat ctcaagatga tatttcaaat gaacaatact
     1261 attgcttgag accattaatg aatgatcata aatatttctt tctataaaat tagctttcca
     1321 ataactgtgt tgttgcataa tatcattcac aagtacacca ttttcggaag tatgattatc
     1381 tttatctata cttaaaacaa tttgtttagt tttagcatgg ctaaattgtt gaagaccctt
     1441 acacgataaa cgtatagcgt ctgaattctc atttaacaat gcggctggca aacaaatgac
     1501 acattgtact tcatgtttga acttcgttac aatcatcgtg tcattttgat aaataacaat
     1561 ccctcgtaat tgattaagta tatttatgat aaaaatgaat attgataatc tagtcaaatt
     1621 tatttctttg ctagcttcac ttataattga catgtgcata ataagtcctc gaaatttagt
     1681 ataatttgtc aaaagttcag taaccttatt aaaatcagat attgtgattt tttcttttaa
     1741 gtcattattc tcattaatca aatctacatc acatataatt ttagaacatg caatagacac
     1801 ctgttctatt tgtcttagcg gtaagataaa atagtctatt agatgatcaa tttctttatc
     1861 atagcgcgta ttcaactctt ttggttccag atgatatatt aattcaaata ctcctatata
     1921 attatctatt aagcgtttca gtttttgaat tgcagtagta tttaagtcgt taattaaaaa
     1981 tgcaatctta catctattac tctcaaatgt catattaaaa attgtggcta ctagcttttc
     2041 tatcttgcct ctttcagtac tcgttaacaa ttccttatat tcatgtttaa tacgaaatac
     2101 tacatttaca ttgtagttca ctatcttttg aagtaataaa gtgatttgtt caatcgatag
     2161 ctcattgctt gtgtcgattg taacaattat atgcaagttt tcaggattaa cacctaatct
     2221 ttgaatgatt tgtttaatag tataataatc catccaataa aaattacttc ctttaatata
     2281 aatgttttta ggttgataca tttgactcct ttttgtaggc tcaaaaggta tatcaatctc
     2341 gcgcatactt gaagaacttt gattagtatc atcaaataat tcaattatat ttttatcaat
     2401 ttcaactaat tgggaatggt taattgaatg agatgttgga ctatatcttt ttctaattaa
     2461 tttaggagtg ttaccatacg tttctttaaa gaggtgtata aaacgagaat aatgattaaa
     2521 accatgacta cttgcgattt cttcaatagg cttcttagaa gttaaaatat caattaagca
     2581 atgctccagt ctaatatgat ttaaatattg aataaaatta ctataaggtg tcgctttaaa
     2641 catgtcactt aaagctttgt ttgtaatact aacttgatta atgacatctt tcctatttat
     2701 ctttttatgg tggttgtttg ttaaataatc gtgcacttct tcggctacta aatgacgact
     2761 accatcgtat gtatttaatg acattaattc aacacacatg taattaataa tcttatcatt
     2821 agcattataa gactgttgtt taattttgct gtagattaaa tacttaatca agattctaaa
     2881 agtgctagca acctcatttg ttaatatctt gccacgcatc atatagtttg cattcataaa
     2941 cttcaaaaat gtctttgcac ttacttcaac aacacaacat acactatcat catgaccatc
     3001 aatttgatac aaatcattca acataataac ggtgacatca tttcttttta catcatattg
     3061 ttgtagattg attgaaacta cacctgtacc ttgtagccaa tatataattt ttaagtttgc
     3121 ttccttcaca ctactcattt tcatactttt aaaaatatgt agtgaataat tatctgtcat
     3181 cttacatcct cacatataca aatatattga tttactcctg tttgtctttc tcaaatcaat
     3241 tgtcaacatc taatattcac taccaattat atcgccacat gttttttagc agtaacaaaa
     3301 cataaatcga ttatatcaaa taatactttt attaaaacat ttttttatgc taaattagga
     3361 tattttatcc attaaatata cattttcatc caaataccaa agctttcata atcatagaaa
     3421 cttgatcgtt cgtgtcgaat ttttatgcat tttctgatta aaagtcctat attatgcaca
     3481 aaatatacat ttagaatttt cagtaatatt acaaatttgt aacagactta ttttcgaaac
     3541 attcagtttt cacaaagaat gccgtagtta taagttttgc aaaatgcaaa tttctatact
     3601 tttaaaattc tattgtgtca agatattagc aaaaaattac aaaacgttaa caaagcacac
     3661 aaggacgcta atttctgtta tagtctttct tgtcgtttaa ttacgagaga taacttataa
     3721 cattaatttt gtcacacgga cattacgaaa atattttaga tttaaaattt ttaggaggat
     3781 atttttaaaa tgaagaaaat cgctacagct actatcgcaa ctgcaggatt cgctacaatc
     3841 gcaattgcat caggaaatca agctcatgct tctgagcaag ataactacgg ttataatcca
     3901 aacgacccaa catcatatag ctatacttac actattgatg cacaaggtaa ctaccattac
     3961 acatggaaag gtaactggca tccaagtcaa ttaaaccaag ataatggcta ctacagctat
     4021 tactactaca atggctacaa taactataat tacaacaatt acaacaatgg ttatagctac
     4081 aataattaca gccgttacaa caactactca aataataatc aatcatataa ctataatagt
     4141 tacaacacaa acagctaccg tactggtggt ttaggtgcaa gctacagcac ttcaagcaac
     4201 aatgttcaag taactacaac tatggctcca tcatcaaatg gccgttcaat ctcaagtggt
     4261 tacacttctg gtcgtaactt atacacttct ggtcaatgta catactacgt atttgatcgt
     4321 gtaggtggta aaatcggttc aacttggggc aatgcaagta actgggctaa cgcagctgca
     4381 agagctggtt acacagtgaa caatacacca aaagctggtg caattatgca aacaactcaa
     4441 ggtgcatacg gtcacgttgc atacgttgaa agtgttaaca gcaatggttc agtaagagtt
     4501 tcagaaatga actatggtta tggcccaggt gttgtaactt cacgtacaat ctcagctagc
     4561 caagctgctg gttataactt cattcactaa ttagtgatga ctaagtttta cgccaacaag
     4621 gtttcaagag ccttgttggc ttttttaatt tctatgaaaa aaatcgaatt ggtaaaaatc
     4681 tcaaaatttc gattttcacc aatccgattc actctgtaat aaagaataaa caacatacag
     4741 ccattgacac acccaaattt aggcatgtca aatcggctat acattgataa atttttagtt
     4801 aaagtactta caaatatact tgccttcaaa ttctcaatta atttagtcat tacaaatcat
     4861 gacataaaac ttacattaca tatatcaaca tgttccgcta caaaacatat tcagtatatg
     4921 tatagaggat atttattcta agttaaaacc ttttcaaatt ataaccgcta caattataaa
     4981 tgattaaagt gtttcaacat attatatata ttccacgctc acattaagtt aaacgtgaat
     5041 tcgatgactt tttaatacct atccctgtaa aaccatagat aattgctata attgcgcata
     5101 aataacatgg tactgtccat atgaaaaatt cttcaacaga gacatgaagt tgattcgtat
     5161 agtaaatacc tgatgttccc catggtatta aaggtaagac catcgtactt gagtcttcaa
     5221 gtgttcttga taaaacggaa cgagagactt ggtatttttc gaacaaatct ttcattaaca
     5281 caccgaccat aataattaca atcgaagcaa cacctgcagc gaatacaagc gcaatacaac
     5341 aaataacagt aatacatatt aaacttccta cagaatggat gcctttagaa atagtagtta
     5401 ataagacttc taaacatcct gctttttcta caatacctgc aaatgcatat ccgcaaaata
     5461 tcgttactaa tatttgggtc atactcatca taccaccctg ttctaacaag cttttcacac
     5521 tggatgaaat atgagactga tgtatcattg agtcgttaaa accactaaat gttgctttga
     5581 aaccatctgt cattttgaaa tgatgattaa aagtccccac tataatggca gaaaagcttg
     5641 atattagcat agctggcact gtagccattt taaatagcaa acaaacaatg atcacaatta
     5701 agggaaccca gacccatatg ttaatttgat aaatctgtgc aagctctgat aacaaagttt
     5761 gaatctgttt atcatttgaa tgccctttaa attgaaatcc agcaataaac catactaata
     5821 aacctatgat tgatgcaggt atcgtcgtcc acatcatcga atgtatatgt ttaaatatat
     5881 taactttagt aacaagcgcc gctaaattag ttgtatctga taatggtgac attttatcgc
     5941 caaacacagc ccctgctata atagcacccg ctgccatccc tggaggaatc cccaattgat
     6001 taccaataga aataagtgca atccctgcag ttgatgcaga gccccatgct gtaccagttg
     6061 ctacagatgt aacagcactt ataaaaaagg ctgatattaa aaaatagctt ggattcaata
     6121 aatctaagcc ataataaatc aaggctggca ctgtgccaga aaacatccaa ctgcctacta
     6181 taattcctac cgctagtata attaaaattg caggcattgc agtatttaaa cgttctgcaa
     6241 tgcctttttc taaatcttgc catgttaagc ctacacgttt tgcaatccat gtggcatatg
     6301 ctgaggcaat aattaataat acttgaattg gaatatcaaa aaacacaaag cctgttacaa
     6361 caacaataac cattacaatc attgtcgaaa ttgattctaa aaaggttggt tttcttttca
     6421 tcatctatcg tccccttcgc ttattgatga gaaacctctt ttcttcaatt tattgctttt
     6481 gcataatgct atatcctttt ctatcttcaa gccatcattt attttaaata tttttctaaa
     6541 tcaagcttat ctaaaccaat ttcttcaact gttaagcctt cctcaaagaa gtctctctct
     6601 aaaatggttg atgcaattac aattactgca tctatatttg gtgtcggtac atcaataaca
     6661 cgacctaagc ttgaccatag tactaaacca tatgcaatat cttcagttaa atatctgctt
     6721 tctacatgat ttggtcctgg aatttgtgaa aatactgggc ttgtattaaa caaacgattt
     6781 aatggttcat cttctttatc acgttctaaa taaccacgtt caatacgtga ttctttagct
     6841 gttgataatt cgaaacctaa tctacggcct aaattcaaac gttctaattc gattgcatga
     6901 agtaatctaa ctgtatgttt agtaattcct tctttatata aagcgaactc gccagcataa
     6961 tcaatgcgac cgacattcaa taatgttggt cctggatgca cttcaggatt accattttca
     7021 agatttgttt taattaagct ttcctctttt actaaatgat cataaatact tgaaactttg
     7081 tcataacaat catttagaca gcttctatca tatgttgaaa agaagatacg acgtacattt
     7141 agagataaat caactgctgc attttcaaaa tcgacacgcg taccatacgt caacgtatta
     7201 gcttccgcta gttgtggttt tgtttcaata tgtctatctt ctaaaacatt cataaaacga
     7261 attgacccca ttgctgcagc catgttgaag aatatcaact gattatcagt tacatgctct
     7321 gccattacat cagcataata ctctatgtat gaagatggaa taatcacttg aacaatttca
     7381 gcatctttta aaacatattc catatcatca ctaatatcat tgaattttac gaaacgttca
     7441 tcaccttcat tattaaaatc aaatccgccc ttttcgattg cgttttgaaa cttacttata
     7501 gattgattac gacaatataa tttaacatcg tggcctttgc tcaccatatc tactgctgcc
     7561 gtaactgcgc catttcctga tcctacaatt gctattttca taaacaaccc tctttctatc
     7621 tttggttaaa attaaaaaca aattatacgt aagacaatat aaaaatgatg cgaattcatt
     7681 acgcatcatt ataaatgtta agtatatata accttaaatt aacctattta aacgccgacg
     7741 tccaccaatt atttttattt ttaataattg tttaataaac atgtttattt ttgattatat
     7801 tttgttatag ttaaagataa tattacaatt ttcaaaggag tgtctaatat gacaactgaa
     7861 atgaacgacg taacaaatca tcaaccgaat cgcaaatcca atgatgagaa cattatggcg
     7921 atgcttatct atttattaag tttattcaca tctattattg gtccactgat tatatggtta
     7981 ttgaaaaaag atgaatcgaa actagttgat cgtgctggaa aaaattatct taactatacg
     8041 atttcttata ttatttggtc tatcgtttta gtcgttatca ctttaatagg cgttttccta
     8101 attgctaccg atattagttt cattatcatt ataggtttca tcattacatt tatcggtatt
     8161 ttatccatat tcgctttctc aatattatct tttgtattca caattattgc gtgtgtc
//
LOCUS       DAHHYJ010000119         8034 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000119 DAHHYJ010000000
VERSION     DAHHYJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.119"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..321)
                     /locus_tag="NBK22_002077"
     CDS             complement(<1..321)
                     /locus_tag="NBK22_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2415258.1"
                     /translation="MHKSNPLLSILKNLKFPKLLLLSALIISSIGSIAGLLVPWFTGE
                     LVNKINGNSLSFNFLVIVISIFLLNAIISGVGLYLLSKIGEKIIFSIRCLVWNHIVRL
                     KTSFF"
     gene            681..773
                     /locus_tag="NBK22_002078"
     CDS             681..773
                     /locus_tag="NBK22_002078"
                     /inference="COORDINATES: protein motif:HMM:NF038165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB family leaderless bacteriocin"
                     /protein_id="HCD2415259.1"
                     /translation="MGAVAKFLGKAALGGAAGGATYAGLKKIFG"
     gene            802..897
                     /locus_tag="NBK22_002079"
     CDS             802..897
                     /locus_tag="NBK22_002079"
                     /inference="COORDINATES: protein motif:HMM:NF038165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB family leaderless bacteriocin"
                     /protein_id="HCD2415260.1"
                     /translation="MGALIKTGAKIIGSGAAGGLGTYIGHKILGK"
     gene            929..1024
                     /locus_tag="NBK22_002080"
     CDS             929..1024
                     /locus_tag="NBK22_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB family leaderless bacteriocin"
                     /protein_id="HCD2415261.1"
                     /translation="MGAVIKVGAKVIGWGAASGAGLYGLEKIFKK"
     gene            complement(1072..1488)
                     /locus_tag="NBK22_002081"
     CDS             complement(1072..1488)
                     /locus_tag="NBK22_002081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415262.1"
                     /translation="MDERQEIINKNMLIRSFGFLFILLIIYIAFLGVINVTITGHQLI
                     LISIITLTSIYMWIDSFINKILYYDVQNNKEIKRKLSSCVTTLLVIDVAVLILAFLNK
                     IDINISFLFLAILISFNIIVLSVYYIILKFWLIWYK"
     gene            complement(1496..1702)
                     /locus_tag="NBK22_002082"
     CDS             complement(1496..1702)
                     /locus_tag="NBK22_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2415263.1"
                     /translation="MNNIKHYRKEMNFSQQELANKVEVTRQTISLIELGKYNPTIKLC
                     ISIAKALQVDLNKLFWEMSDNSEE"
     gene            2363..2746
                     /gene="mobC"
                     /locus_tag="NBK22_002083"
     CDS             2363..2746
                     /gene="mobC"
                     /locus_tag="NBK22_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCD2415264.1"
                     /translation="MSEQNIFVASDTVNGQIHKPNRKAPKQISFRVSESEYLKLKQSA
                     ETLNMSVPAFVKKKAQGTRLVVPKLDKTTRQSLASDLGKLGSNANQIAKYCNQHQYDA
                     PNYEALERNISELRERLDDIWQQLN"
     gene            2728..3735
                     /locus_tag="NBK22_002084"
     CDS             2728..3735
                     /locus_tag="NBK22_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCD2415265.1"
                     /translation="MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASR
                     ELYGKTDGNQGHVIIQAFKPDEVTPEQCNQLGLELAEKFAPNHQVAVYTHNDTDHVHN
                     HIVINSINLETGKKFNNNKQALRDLRNFNDEVCRNHGLSVPEKDTARLRYTQTEKAIA
                     DPNIKSTAQYSWKDEIREAIDQSQATNMDELKTHLSQYGIEIERVTPKSITYRHLAED
                     KKVRGRRLGENYNKGGIEDGFERQIQRRQQERDSEHDYQPKRRTSNRDESTERDTSIT
                     QSDWDRFAKDTNELERSRKAAESARLAHEKARRDREERIQAERATRKNIDNDRDHGYE
                     L"
     gene            3776..4252
                     /locus_tag="NBK22_002085"
     CDS             3776..4252
                     /locus_tag="NBK22_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCD2415266.1"
                     /translation="MKHYIQRIDSDNLKQDFKTAMQEELKDIKSETENMVKQFQVSQI
                     ELQTSNNEYAKTIEARLNKNETAIKQYDQAVNKLTKGLTSMFFIVALVMVACLVLSPL
                     SDVLGVHYFYEWINHVLKTGHSAWRYFIVILYLIPYILLWLLIRGILSAYKRVSSF"
     gene            4967..5824
                     /locus_tag="NBK22_002086"
     CDS             4967..5824
                     /locus_tag="NBK22_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCD2415267.1"
                     /translation="MSGETVVYQNEMNLVPLRKFTSTEIDIFFSLCNKLKEQDIKELA
                     IPFEELRHLSNYYHRSLKRFVKDLEHVYDKMLTLTYTERSGLSFKKFVLFTGYEVDVD
                     EKKLIVSINPKLKHVLNEITADFTKFELKEMTNLKSTYSKNMFRLLKQYKHTGYFKIQ
                     IEDFRERLDIPNSYRMTHINQKVLAPIIKELGFIFNNLNINKVKAKKGRKIEWLEFTF
                     DAEKRIHNKREPQMANIGKSRQYISREKTPKWLEERTYEKTPQREYDPQLEKEREAFL
                     KQLEMNWEE"
     gene            complement(5966..6061)
                     /locus_tag="NBK22_002087"
     CDS             complement(5966..6061)
                     /locus_tag="NBK22_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD2415268.1"
                     /translation="MTLLFVYVIAPVFVGCCITLFQFWLEQRSKK"
     gene            6210..6395
                     /locus_tag="NBK22_002088"
     CDS             6210..6395
                     /locus_tag="NBK22_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415269.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIHFLNKNGEWYYFEVRSVWRGRHI
                     IRVKDGLLGWRKEIVTE"
     gene            complement(6640..>8034)
                     /locus_tag="NBK22_002089"
     CDS             complement(6640..>8034)
                     /locus_tag="NBK22_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2415270.1"
                     /translation="DSNESGELLSRIIDDTKVINTFVSHKLPSTLPNILTIIGSIIML
                     FILDWKMTLCTFVIVPLFFMIILPLGQMMEKTSNKTQNEIAQFSGMISRILSEIRLVK
                     VSSTENLEVKNGKDRLTNIYNLGIKQAKITSIIEPISSILVLLMIAIVLGFGGWRVSS
                     GNISSGNLVAMIFYVFQLVLPISNISTLITDYKQAKGASFRLSEILSQKTEEIEGKRN
                     EDLDIPFIKFENVTFSYDKEEILKNLSFTIPQNKVTAFVGPSGSGKSTIFNLIEKMYD
                     IDNGKIYIGNSLLSDLDLLSWRKNLGYVMQDNSMMSGTIRDNILYGLEDQEIKDDYLD
                     YYCKLANCYDFVNNLEERYNTFIGERGVKLSGGQRQRIDIARSFIKNPKVLLLDEITA
                     NLDSESEHKIQDAMSTLMRDRTTIIIAHRLSTIQKADQIIFLDNGVITGMGDHNSLYN
                     SHKKYKEFVLNQHI"
BASE COUNT         2951 a         1217 c         1230 g         2636 t
ORIGIN      
        1 aaaaaaagaa gtttttaatc gaacaatatg attccatact aaacatctaa ttgaaaaaat
       61 gattttttca ccaattttac ttaataaata taaaccaaca ccacttataa ttgcgtttaa
      121 taaaaatata gaaataacta taactaaaaa attaaaactt agtgaatttc catttatttt
      181 atttactaat tcccctgtaa accatggtac taatagacca gcaatactac caatagacga
      241 tataattaaa gctgataata atagaagttt aggaaacttt aagttcttca aaatacttaa
      301 taatgggtta ctcttatgca aattagtcac tcctataatt acaaaattaa aagcacatgt
      361 tacttaaacc gaataaaccc atagcaacat atgctttatc ataaattatt tgaaaataca
      421 taatgatagt tatgtataat aataatatca atatatagtt tttacaatac ctaattattt
      481 tataaataaa tatcttgata ttaaaacaaa ctaataataa aatattaaca agagaaaaaa
      541 gggaggtgtt acaatgggga aattagctat caaagctgga aaaatcattg gcggtggtat
      601 cgctagcgct cttggttggg ctgctggaga gaaagctgta ggtaaataat tctataaata
      661 attttcgagg aggtgttttc atgggtgctg tagcaaaatt tcttggtaaa gcagctttag
      721 gtggtgctgc tggtggcgca acttacgcag ggttaaaaaa gatttttggt taatatttaa
      781 aaaatttaag gagttattta catgggtgca ttaattaaaa ctggtgctaa aatcattggt
      841 agtggtgctg ctggtggttt aggtacatat attggtcata aaattctagg taaatagcgt
      901 ttacttaaaa agcaaaggag agaaaactat gggcgcagta attaaagtag gtgctaaggt
      961 cattggttgg ggagccgcta gtggcgcagg tttatatggt ttagaaaaaa tttttaaaaa
     1021 ataactaagc attcgagtat aaggtttaat caaacctcat actcgatttt tctatttata
     1081 ccaaattaac cagaatttta atattatata atatacactt agtactataa tgttaaagga
     1141 tattaatata gctaaaaata aaaatgaaat attaatatca atcttattta aaaaagccaa
     1201 tattaaaact gcaacatcaa taactaacaa tgtcgtaacg catgatgaca actttcgttt
     1261 tatctctttg ttattttgaa catcataata caaaatttta tttataaaag aatcaatcca
     1321 catatatata ctagttaatg taataatact gattaatatt aattggtgtc ctgttatagt
     1381 tacattaatc acgcctaaaa aagctatata aattattaat agtataaata aaaagccaaa
     1441 agacctaatt aacatattct tattaattat ttcttgtctt tcatccatat ttctattatt
     1501 cctcactatt atcactcatt tcccaaaata atttattcaa atctacttgt aaagctttag
     1561 ctatgctaat acataatttg atagtaggat tatatttccc taattcaatt aaagatattg
     1621 tctgccttgt tacttcaact ttattagcca actcttgctg cgaaaaattc atttctttac
     1681 gataatgctt aatattattt atcatgatta taaactcttc ctttttcaca cacacttttg
     1741 ccatatatat attgcattta taatgtaaca aatctatatt aattatgcaa taccctcgtc
     1801 attataaagt taaaatgaga ttaccttgaa aattaaaacg tattataaac gtaagtattt
     1861 agggtgttta taaactaatg tttgattatg ggtaaaatat ataatttatt tgtttgcttc
     1921 aagtattttt tgtattcttt ataagaatat ggggtgtcta attttttcat caatgtatac
     1981 tgcaggaact tcctcgctat atatcattgt tttttcttac ttaaccttat ttctgaaatc
     2041 aaatttatat tttttgaaat attaaacatt tcagtaatta gtattcttgt aaggattatt
     2101 tttaccgata tcaagaaaaa aagctaaaca tctttaaata catttatatt aatagtggct
     2161 agcaattagc cactattttt tcgccagaaa aaatcctaag gggcttgggg aatatcccca
     2221 acaagcaggc gatgctacca cgttagtggc tagcaaagcc aatgcttgcc aaaccactat
     2281 aacgacttga ttaaatttac tttatcctat aaagtaaatt tatattaagg agttatcttt
     2341 cgtagaaagc gtggtgggat gtatgagcga acaaaacata tttgtggcta gcgatacggt
     2401 taatgggcaa attcataaac ccaaccgtaa agcgccaaaa caaattagtt ttcgtgtgag
     2461 cgaatccgaa tatttaaagt tgaaacaatc ggctgaaact ttaaatatga gtgtgcctgc
     2521 tttcgttaag aaaaaggcac aaggcactcg attagttgta cctaaattag ataaaactac
     2581 acgacaatct ctagctagtg atttaggtaa attaggttca aatgccaatc aaattgccaa
     2641 atattgtaac caacatcaat atgacgcacc aaattacgaa gcattagaac gtaatatcag
     2701 cgaattacgt gaaaggctgg atgacatatg gcaacaacta aactaagcgc aaccaaatca
     2761 acatctcgtg ctattaatta cgctgaaaaa cgcgctgttg agaaaagtgg cttaaactgt
     2821 gatgtagatt atgctaaatc gagtttcaaa gcgtctcgtg agctttacgg taaaacagat
     2881 ggcaaccaag gtcatgtcat tatacaagcc tttaaacctg atgaagttac acctgaacaa
     2941 tgtaatcagc taggtttaga actcgctgaa aaatttgcac cgaatcatca agtcgctgtc
     3001 tatacacaca atgatacgga tcatgtacat aatcatattg tgattaatag tattaatctt
     3061 gaaacaggta agaaattcaa caacaataaa caagcattac gtgacttaag aaatttcaat
     3121 gacgaagttt gtcgcaatca tggtttaagc gtaccagaga aagatacagc acgcttacgt
     3181 tatacccaaa ctgaaaaagc cattgcggat ccaaacataa aatcaacagc acagtattca
     3241 tggaaagatg aaattcgaga agctatcgac caatcacaag caacgaatat ggacgaatta
     3301 aaaacgcatt taagtcaata tggcattgaa attgaacgtg tcacaccaaa aagcattacg
     3361 tatcgacacc tagctgaaga taaaaaagta cgggggcgtc gattaggcga aaattacaat
     3421 aaagggggca tagaagatgg ctttgaaaga caaatacaac gacgacaaca agaacgagac
     3481 tcagaacacg actaccaacc gaaacgacga acttcgaatc gtgatgaatc aactgaaaga
     3541 gatacaagca tcactcaatc agattgggat cgattcgcca aggatacaaa tgaattggag
     3601 cgtagccgaa aagctgcaga atcagcaaga cttgctcatg aaaaggctcg aagagatcga
     3661 gaagagagaa ttcaagcgga acgagcgaca cgaaaaaata ttgacaacga tcgagaccac
     3721 ggctacgaac tttgatcaag cgacaaagac taatgaaaaa cgatttatta gcgcaatgaa
     3781 acactatatt caacgcattg atagtgataa tttaaaacaa gatttcaaaa cagccatgca
     3841 agaagaacta aaagatatta aatcggaaac agaaaatatg gttaaacaat ttcaagtgag
     3901 tcagatagaa ttacaaacat ccaataatga atacgcgaaa accattgaag cacgcttaaa
     3961 caaaaatgaa accgcgataa agcagtatga tcaagcagtg aataagttaa cgaaaggatt
     4021 aacttctatg tttttcatcg ttgctttagt aatggtcgca tgtttagttt taagtccgtt
     4081 aagcgatgta ttaggtgttc attattttta tgaatggatt aatcatgtac tcaaaacagg
     4141 tcattcagca tggcgttatt ttatcgttat cctttattta atcccttata tcttattgtg
     4201 gttgcttata cgtggcattt taagcgcata taagcgcgtt agttcatttt gaataggtag
     4261 agaatcatat caacgtatag aagtcctaca tgaagctgaa aatggatttt aaaagcattt
     4321 aaataataat gactatttaa atccatcgtt atgagaagcg tattaaagat tagatatcgc
     4381 tattatgccg agaaaattta ttgatcgttg agaagtcccc ttaactaaac tgtttcgacg
     4441 aatgtcgggt atagcgtgag ctattaaccc gatgagtcga cagtttttgg gtttgttaag
     4501 gggacagagg ctcaatgtca ataaagcaat tggaataata catcaatgag taacgactta
     4561 attgatttta taaataaaaa agcaaccact tttgtggttg ctttcctttt tgtctcttga
     4621 atttgtgagg ggaaaattgc cccgaacttt ttaatatctt tttataaatc tttttaaaac
     4681 cctttttagg gggtgtttga accttagagg gacaatgcat ttgagatttt aaaggcaact
     4741 ttttgtaacc ataaaggcaa ctttttgtaa ccataaaggc aactttttgt aaccataaag
     4801 gcaacttttt gtaaccataa aggcaacttt ttaatagcga gttgccttta tggttacaat
     4861 tatgctatta taggttttaa ttgaattttt cttatatcat taaaagacat cttattatta
     4921 ataaagtgtc ttttaatcca cttattcttt aggaggtttt ataaacatgt ctggagaaac
     4981 tgttgtatat caaaatgaaa tgaatttagt tccattacgt aagtttacca gcacagagat
     5041 tgatatattt tttagtcttt gtaataagtt aaaagaacaa gatataaaag aattagctat
     5101 cccttttgaa gagttacgtc atttaagtaa ttactatcat cgctcattaa aacgttttgt
     5161 taaagactta gaacatgtat acgataaaat gttaactcta acttatactg aaagaagtgg
     5221 tctatctttc aaaaaatttg tattatttac tgggtatgaa gttgatgttg atgagaaaaa
     5281 attaatcgtt agtattaatc ctaaattaaa gcatgtttta aatgaaataa cagctgattt
     5341 tactaaattt gaattaaaag aaatgacaaa tcttaaatct acatactcta aaaatatgtt
     5401 tagattatta aaacaatata aacacacagg ttattttaaa attcaaattg aagattttag
     5461 agaacgatta gatattccta atagttatcg catgactcat attaatcaaa aagtattagc
     5521 tcctataatt aaagaattag ggtttatctt taataacctt aatatcaata aagttaaagc
     5581 caaaaaaggt cgtaaaattg aatggttaga gtttactttt gacgctgaga aacgcatcca
     5641 caataaacga gaaccacaaa tggcgaatat aggtaagtca cgacaatata tcagtcgtga
     5701 gaaaacacca aaatggttag aagaaaggac atatgaaaaa acaccccaaa gagaatatga
     5761 tccacaacta gaaaaagaaa gagaagcctt tttaaaacaa cttgaaatga attgggaaga
     5821 ataaaaatgt attaaacgat aaataataat tgaagaatgt gtaataaaat gatactattt
     5881 agacaacaaa tagtggtgtc attaacgcac cgaaccccca acctatggac gtaggttggg
     5941 ggttctttta gtgttggctt gtcgcctatt ttttactacg ttgttctagc caaaattgaa
     6001 acaacgtaat acagcatcct acaaaaacag gagctattac ataaacaaat agtaatgtca
     6061 tttagcgcac ctcctctcta cgtcaaatcg acgtctgaga gatagacgac tttcacatta
     6121 taacaaatct ctccttccat gaaataacat gaatattaat aaatgttgtt atatattaaa
     6181 gataaattaa tataagggtg ggattgatga tgtttggcga tcaaagacaa gaagctacaa
     6241 aatatgtcat taaagaaggt tatcaagata ttcatttcct caacaaaaac ggggaatggt
     6301 attatttcga agtaagaagt gtctggcgtg gcagacatat tattagagtt aaagatggtt
     6361 tgttaggttg gagaaaagaa atcgtaactg aataaagata caaaaactta agttatgaaa
     6421 aagaatgaaa acgtgtcatt tagggtaatg tataaaagat gaatacccgc taagattgat
     6481 aaaaagcccg aaacactgat atgaaagtgt ttcgggcttt tcttttagtt aacataatgt
     6541 tcaattatgg gtagttgttt cgtatatagt attcactgta ttaattttat tctgatcact
     6601 caatataaca aagtgtatat atctgatata tacactttgt tatatatgct ggtttaacac
     6661 aaactcttta tactttttat gactgttata taatgaatta tggtccccca tacctgtgat
     6721 tacaccgtta tctaaaaata ttatctgatc agccttttgg attgttgata gcctatgtgc
     6781 aataataatt gttgttctat ctctcattaa agtagacatt gcatcttgaa ttttatgttc
     6841 actttcacta tctaaatttg cagtaatttc atcaagtaat agtactttag ggttttttat
     6901 aaaacttcta gcaatatcta ttctttggcg ttgccctcca gataacttga ctcctctttc
     6961 tccaataaat gtattatacc tttcttctaa attattaaca aaatcataac aatttgctaa
     7021 tttacagtaa taatctagat aatcatcttt aatttcttga tcttctaatc cgtataaaat
     7081 attatctcgt atagtgccac tcatcattga gttatcttgc attacatatc ctagattttt
     7141 tctccaactc aataaatcca aatcactcaa taatgaatta cctatatata tttttccatt
     7201 atcaatgtca tacattttct caatcaaatt gaatatagta ctttttccag atcctgaagg
     7261 tccaacaaaa gctgtcactt tattttgtgg tattgtaaaa cttaaatttt tcaatatttc
     7321 ttccttgtca tatgaaaaag taacattttc aaatttaata aaaggtatat ctaaatcttc
     7381 atttctttta ccttctattt cttcggtttt ttgacttaat atttctgata gcctaaagct
     7441 agctccctta gcttgtttat aatcagttat tagtgttgaa atattactaa taggtaaaac
     7501 taattgaaat acataaaaaa tcattgctac taaatttccc gatgatatat tcccacttga
     7561 tactctccag cctccaaacc ccaagacaat agctatcatc aataaaacta aaatgctcga
     7621 tataggttct attatagatg ttattttagc ctgctttatt cctaaattat aaatattagt
     7681 taatcgatcc ttaccatttt tgacttctaa attttctgta cttgaaactt taactaatct
     7741 aatttccgat aaaattctac ttatcattcc actaaattga gcaatttcat tttgtgtttt
     7801 attagaagtt ttttccatca tttgtcctaa aggcagtata atcataaaaa ataaaggaac
     7861 aataacaaat gtgcatagag tcattttcca atctaatata aaaagcataa ttatcgatcc
     7921 aataattgtt aatatattag gtaaagtgct aggaagtttg tgtgatacaa aagtatttat
     7981 tacttttgta tcatctatta ttctacttaa tagctctcca ctttcatttg aatc
//
LOCUS       DAHHYJ010000120         8003 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000120 DAHHYJ010000000
VERSION     DAHHYJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.120"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(79..585)
                     /locus_tag="NBK22_002090"
     CDS             complement(79..585)
                     /locus_tag="NBK22_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCD2415271.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFYKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPDFERIIQTNYPDYDYNHETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            677..1408
                     /locus_tag="NBK22_002091"
     CDS             677..1408
                     /locus_tag="NBK22_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCD2415272.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            1401..1943
                     /gene="scpB"
                     /locus_tag="NBK22_002092"
     CDS             1401..1943
                     /gene="scpB"
                     /locus_tag="NBK22_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCD2415273.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKNQLVELIDNYSS
                     HGLMIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            1936..2673
                     /locus_tag="NBK22_002093"
     CDS             1936..2673
                     /locus_tag="NBK22_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD2415274.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            2806..3531
                     /gene="srrA"
                     /locus_tag="NBK22_002094"
     CDS             2806..3531
                     /gene="srrA"
                     /locus_tag="NBK22_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCD2415275.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            3512..5263
                     /gene="srrB"
                     /locus_tag="NBK22_002095"
     CDS             3512..5263
                     /gene="srrB"
                     /locus_tag="NBK22_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCD2415276.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSMVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKNIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAVVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENESED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
     gene            5508..6424
                     /locus_tag="NBK22_002096"
                     /pseudo
     CDS             5508..6424
                     /locus_tag="NBK22_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
     gene            6427..8003
                     /locus_tag="NBK22_002097"
                     /pseudo
     CDS             6427..8003
                     /locus_tag="NBK22_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723487.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3068 a         1032 c         1478 g         2425 t
ORIGIN      
        1 aagtgaacca taactcaaaa gtcacaatac acatgactaa aaatgtctgt gctattgtgg
       61 cttttttaaa ttggttgatt aattacgtct atgttttctt aattgaatcg cttcttcttt
      121 tgctgcaatc acttctgaac gatcacgccg catgtgatgg tctacaataa aaggatctgt
      181 tgctgtttcg tggttataat cataatctgg atagttggtc tggatgatgc gttcaaagtc
      241 tggagttatt ggtaatataa cagatgaaaa aggctttgct gcattcaatt ttgcaatctg
      301 ttgctcaatt aacaactgat aatcatttaa attaaggtat aatgcatctc tatctttagc
      361 attttgtatt atttctttag atttataaaa agacttatag gcgcctttta aattattgcg
      421 gcgataatgg taacaagcag ttgcaaacaa gattaaacta acaactgcat cttgcttact
      481 gtagttattt tcagctttcc atgcatcttc taaaatgtca tgacatagga aataatgttg
      541 cttagtatga aattgataat agaaatttat cagtgcctgt tgcattttgt tatcacccca
      601 atttaaaagt aagttatttt catgctataa tattttagag aattatgcac atatgacgca
      661 atatgaggta gatattatgt atgaagttaa attagatgct ttcaatggac cattagattt
      721 attactgcat cttatccaaa aatttgaaat agatatttat gatattccta tgcaagcatt
      781 aacagagcag tatatgcagt acgttcatgc aatgaaacag cttgaaatta atattgcaag
      841 tgaataccta gtattagcgt cagaactctt aatgattaaa agtaagatgc tattaccaca
      901 atcaacatca gatatggatg ttgatgatga cccacgggaa gatttagttg ggcgtttaat
      961 agaatatcaa aattataaag aatatactgc tattttaaat gacatgaaag aagaaagaga
     1021 tttttatttt acaaaaagac cgacagattt atctcatttg gaaacagatg aatcttggga
     1081 tccaaatcat acgattgatt taactgaatt aattgtagct tatcaaagag ttaaaaatag
     1141 agttgagtta aatacaccta aatctgttga aatccggaaa gagactttta ctattcaaca
     1201 agctacagaa caagtgacat cgagattgaa agataaagat cattttaact tctttagtct
     1261 gtttacgttt tctgagccaa ttgaacaagt agtcactcac tttttagcta ttttagagat
     1321 gtcaaaagca ggaataatta atattgagca acaacgtaat tttgaagata ttaacattat
     1381 tagaggagtg aactaccatt ttggataatc atggtatatt agagtcgctt ttatttacag
     1441 ctggcgatga aggtttagat gaaaaacaac tattagaaat attagatatg tcgaaaaatc
     1501 aactcgttga attaattgac aattattcat cacatggatt aatgatacaa cgatttggaa
     1561 cgacgtatgt tttaacgact aaaaaagaag cggcaacgta tattgaacaa ttaattgaac
     1621 aaaagtcaca aatgaaatta tcacaagcag caatggaagt actatcaatt attgcttata
     1681 accaaccatt atcaagaagt gatattgaat taattcgtag tatcaattca gatggtgcag
     1741 ttaagacatt gattgccaaa ggactagttg aggctaaagt cgtcaatgaa cagcgtagcc
     1801 aacagttaat tactactgat ttatttttaa atgtatttgg tatttccaat atagaagatt
     1861 tgcctacaac tgaagaagac gatgaagaaa tggatgcttt tttcagtaat ctagtcaatc
     1921 aaaaaggaga aaataatgac taaagaatta gaacgattac aaaaacgtat agctaatagc
     1981 ggttatactt caagacgtaa ggcagaaact ttaatttctg aaggaaaagt gaaagtgaat
     2041 ggtacgactg tcactgaatt aggaacaaaa gtgaagccat cagatacgat tgaagttgaa
     2101 ggtattaaaa tagaactaga agacaaaatt tacattttat tccataaacc aactcaagtg
     2161 ataacaagtg tatctgatga tagaggacgt acagtggtta cagattactt taaagatata
     2221 gaagcacgta tttatcctgt tggtcgttta gactatgata cttctggatt gttattactt
     2281 acaaatgatg gtgaatttac taatttaatg acacatccaa gatatcaaat taagaagaaa
     2341 tatgtagcaa aattaaaagg ttacttaatg agagaagaag tgaaagccct agaaaaaggt
     2401 attgaattag aagatggtat gacgcaaccg gctgaagtga aagttaagaa acaagataaa
     2461 gataaaaaca caacattggt tgaaattaca attacagaag gtcgtaatcg ccaagtcaga
     2521 agaatgttcg aacattttgg tcatcaagtg acgaaactgt ctcgtattga atatgggcct
     2581 ttaaatgttg tcggtttgaa tgctggtgaa ggacgtgtat tgacgcctca tgaagtaaaa
     2641 gtgatgcgtc atttagcaga acatgggaaa taattaaata aaatatgtat ttatcacaaa
     2701 gtttgagaat tttttcacaa aattttaggt ttttaataac tgtatgcgct ttcctgtgat
     2761 ataatacttt tgtaaagaaa agcatgtgtg ggaggtatga cctgtatgtc gaacgaaata
     2821 cttatcgtag atgatgagga tagaatcaga agattactta aaatgtattt agaaagagaa
     2881 tcttttgaaa tccatgaagc aagtaatggc caagaggctt atgaacttgc aatggagaat
     2941 aattatgctt gcatactact agatttaatg ttgcctgaaa tggatggtat ccaggtggca
     3001 actaaattgc gtgaacataa acaaacaccg attattatgt tgactgctaa aggtgaagaa
     3061 acaaaccgtg ttgaaggttt tgaatctggt gcagatgatt atatcgtcaa accattttca
     3121 ccaagagaag tagtcttaag agttaaagca cttctaagaa gaacgcaatc tacaactgta
     3181 gaacaaagcg aacctcacgc acgtgatgtg attgaattta aacatttaga aatagataat
     3241 gatgcacatc gcgtacttgc tgataatcaa gaagttaatt tgactcctaa agagtacgaa
     3301 ttattaatat atttagctaa aacaccaaat aaagtatttg accgtgaaca attattaaaa
     3361 gaagtttggc attatgaatt ctatggtgat ttaagaacag ttgatactca tgttaaacga
     3421 cttagagaaa agttaaatcg tgtgtctagc gaagctgcgc atatgattca aacagtctgg
     3481 ggcgttgggt ataaatttga ggttaaatct aatgatgagc cggctaaata gtgtcgtaat
     3541 taaactgtgg ttaactatta ttttaatagt gacgacagtt ttaattttat taagtattgc
     3601 tttaattacc tttatgcaat actatttcac acaagaaacc gaaaatgcca taagagaaga
     3661 tgctagacgt ataagttcaa tggtcgaaca atcacataat aaagaagaag caataaaata
     3721 tagtcaaaca ttaattgaaa atcctggtgg gttgatgatt ataaataata aacatcgtca
     3781 atcaacggct tcactttcta atattaaaaa gcaaatgttg aatgaagtag tcaacaacga
     3841 ccattttgac gatgtgtttg ataaaggtaa atctgttact cgaaatgtaa cgattaaaga
     3901 aaagggttca tctcaaacat atattttgtt aggctatcca acaaaagcac agaagaatag
     3961 tcatagcaaa tatagtggag tctttatata taaagacttg aaatcaatcg aagatacaaa
     4021 taatgctatt acgattatca ctataattac ggctgttatt ttcttaacaa ttacaacagt
     4081 ctttgcgttt ttcttatcgt caagaattac aaaaccttta agacgtttaa gagaccaagc
     4141 tacacgtgta tctgaagggg attactctta taaaccttct gtcacaacga aagatgaaat
     4201 tggtcaatta tcgcaggcat ttaatcagat gagtacagaa atcgaagagc atgtcgacgc
     4261 attatccaca tctaaaaata ttagagacag cttaattaac tctatggtag aaggtgtcct
     4321 aggtattaat gagagtcgac aaattatctt atctaataag atggcgaatg atattatgga
     4381 caatattgat gaagatgcta aagctttctt attaagacaa attgaagata cttttaaatc
     4441 aaaacaaacg gaaatgcgtg atttagaaat gaatgcacga ttctttgttg tgaccacaag
     4501 ctatatcgat aagattgaac agggaggtaa aagtggtgtt gttgtgacag ttcgtgatat
     4561 gactaatgag cacaatctag atcaaatgaa gaaagatttc attgctaatg tatcacatga
     4621 attacgaaca cctatatcat tactacaagg ttatactgaa tcaattgtag atggtattgt
     4681 tacagaaccg gatgaaataa aagaatcgct cgccgttgtc cttgatgaat cgaaacgttt
     4741 aaatcgatta gttaatgaat tgttaaatgt cgcacgcatg gatgctgaag ggttatccgt
     4801 aaataaagaa gttcagccta ttgcagcgtt actagataag atgaaaatta agtatcgcca
     4861 acaagctgat gatttaggtc taaatatgac ttttaattat tgtaagaagc gtgtttggag
     4921 ttatgatatg gatcgcatgg accaagtact aacgaactta attgataatg catcacgtta
     4981 tacgaaacct ggagatgaaa ttgcaattac ttgtgatgaa aatgaaagcg aagatatttt
     5041 atacattaaa gatacaggca caggcattgc accagaacat ttacaacaag tatttgatcg
     5101 tttttataaa gttgatgcag cgagaacgcg aggtaaacaa ggtaccggtt taggtttgtt
     5161 catttgtaaa atgattatcg aagagcatgg tggttccatt gatgttaaaa gcgaattagg
     5221 aaaaggcaca acatttatta ttaaactacc aaaaccagaa taaaactgaa tatagttatt
     5281 taagaacgca tgttattgat tagagactct aatttatagc atgcgttttt tgattgatgt
     5341 ggggaatttt gtatgtggat tacgatttag gatttttgtg aataacaact attaaatata
     5401 ttactaattt atacaaaaat ataaagtttg ataaagttat ttatttgatt ataaaaatag
     5461 ggtaaaatat agatatattg taataattaa attattcgag gtgtcatatg aaaaaattca
     5521 ttggatcagt tttagctacg acattaattt tagggggatg ttccacgatg gaaaatgaat
     5581 caaaaaaaga cacaaaaaca gaaacaaaat ctgtaccaga agaaatggaa gcttcaaaat
     5641 atgtaggtca aggcttccaa ccacctgcag aaaaagatgc gattgaattt gcgaagaagc
     5701 ataaagataa aattgccaaa cgaggcgaac aattttttat ggataacttc ggtctaaaag
     5761 ttaaagctac caatgttgta ggtagtggcg acggtgtaga agtttatgtg cattgcgatg
     5821 accacgatat cgtatttaat gcgagtattc catttgataa atcaattatt gacagtgata
     5881 gctcattaag aagtgaggat aagggcgatg atatgagtac tttagttggt acagtactaa
     5941 gtggatttga atatcgagca caaaaagaaa agtatgataa cctatataaa tttttcaaag
     6001 ataacgaaga gaaataccaa tatacaggat ttacaaaaga ggcaattaac aagacacaaa
     6061 atagtggata tgaaaatgaa tatttttata tcgttgcaaa tataccaaca cttcaagaat
     6121 atagaaaata ttacgaacct ctgattaaaa aaataatcta gattttaaaa aaggtatgaa
     6181 agatgctaga agaggagtag gctacaaagc tgaaatcgaa ttatatacaa cactgttttc
     6241 gaaaagtaga aacttttcaa aggacaagaa attagatgat gctatagatt tatccgaaag
     6301 cgtgaaaaag ttaaacctta atttagaaaa cacgaaaata tttttacaac taacgaaatc
     6361 taccattaat actaaacaag taaattatag tgataacaaa cctatcaaga ttgaggttga
     6421 gtgaaaatga acaatataaa cgatagagat ttaactgagt taagtggtta ttgggtttac
     6481 caagatatta aagatggtag taaatttaga gttaacgata aaaaattcaa acaagtcgaa
     6541 gagataacga aagtagctaa taaaaataat agttcagata taaaagttta tgaactattg
     6601 gatgaaaatg aaacacctac aggcaaacaa gtattattat ttcaaggtac agataaccaa
     6661 aagagtgatg tgaaaagcaa tccttttcgt gggaaggttg ctgatgattg gattgaaaat
     6721 ataaagttaa tgaatgataa aaataagtca acttctttat tagaacaaaa taatgcttat
     6781 attaaatcgt atgaacagaa actaaaagat gcagatacac ttaaaagctc tgatttttta
     6841 aggaaatata agcaaaatcc gtctacgtat aaaaacaaaa ctattaagtc tgatggaggc
     6901 aattctcaag gggggagcaa gtgctaacca ccaagggttg attaacccga ataaaaatat
     6961 agtttctaca aaccctgcca tgttaccaaa gtcaatatgg gaaaatttca aatctcaaaa
     7021 ctttgaaaat atgattaatt atcatagtaa gtttgatatt ttatcttggc tgcaggatcc
     7081 atttgccact ccgacactag gaaaacgagt gaatttagaa actggtgtgc caacgatgga
     7141 tggattagta aatagtcatt taggttataa aagaaaatta aatccaagat ataatactta
     7201 taaggattta gcagtttata aaattaaatc agttaaagat actatgatta aaaatggtga
     7261 aaaagtcaaa aaaactattg aaatcgatat aaatatggat gatagaattc caattaatgt
     7321 gtggacagga gattcaatag cgagaacggg taaagggact cctataaaat taaatctaga
     7381 aaatcttagt gcattaagta atttggtaac tggtgaaaca agtaatatgt taaccgattg
     7441 tgtaaattat ttgaatgaga gctataatat ttcacaggtt gaaaattcta attttggaga
     7501 aagaaaacat aaacttaaac aagattttaa aaagattgtc gaagttgata tccttgaagg
     7561 aatgagtagg gaattaactt cttttaaaca ggatgtattt agcgcaattg atgatgtcaa
     7621 aaaaaatctt gttgtaataa tcttaatagc tccagcagct ggagtcctta ttcttgaatt
     7681 aaatactatt gaagaaattc ttgaagattt agataaaaaa ttacaacgtg gcatagaaag
     7741 tttacatgat tcacttgata acgtaattaa agaaatgttt aaaaatttag atcatgattt
     7801 tgaagatggc gtgacagagg aaatgatgaa gcatttaaat gttgtgaata ataatataaa
     7861 ttttgtgaaa aagcaaaatg atgtatatgg agagcagata accgatatta aaaatataat
     7921 gtctcatcaa gatgcaacgg taatggatgg aaacttatct attaattata gcggtgaaca
     7981 tatgatttcg ggtcaagtac aat
//
LOCUS       DAHHYJ010000121         7918 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000121 DAHHYJ010000000
VERSION     DAHHYJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.121"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            236..1273
                     /locus_tag="NBK22_002098"
     CDS             236..1273
                     /locus_tag="NBK22_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD2415277.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1324..2154
                     /gene="sph"
                     /locus_tag="NBK22_002099"
                     /pseudo
     CDS             1324..2154
                     /gene="sph"
                     /locus_tag="NBK22_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2392..3408)
                     /gene="lukG"
                     /locus_tag="NBK22_002100"
     CDS             complement(2392..3408)
                     /gene="lukG"
                     /locus_tag="NBK22_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCD2415278.1"
                     /translation="MIKQLYKNITICTLALSTTFTVLPATSYAKINSEIKAVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRIQDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAHLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKKDEGKSKF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(3430..4482)
                     /gene="lukH"
                     /locus_tag="NBK22_002101"
     CDS             complement(3430..4482)
                     /gene="lukH"
                     /locus_tag="NBK22_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCD2415279.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKDKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNPKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKEGQRLIVTYEVDWKNKT
                     VKVVDKYSDNKSFREG"
     gene            4917..6140
                     /locus_tag="NBK22_002102"
     CDS             4917..6140
                     /locus_tag="NBK22_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCD2415280.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRG
                     TTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSE
                     LKKHDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDDIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            complement(6565..7356)
                     /locus_tag="NBK22_002103"
     CDS             complement(6565..7356)
                     /locus_tag="NBK22_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD2415281.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSDILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKG"
     gene            complement(7418..>7918)
                     /locus_tag="NBK22_002104"
     CDS             complement(7418..>7918)
                     /locus_tag="NBK22_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2415282.1"
                     /translation="EILKEIGKLTNNEDKAKKWIEEWDDKTRKDKKEIQSKIGQATAS
                     VFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKDKLQEDKKGYASISKENISKYA
                     GDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYKAEDYWFTDPITLEHLRSKLKK
                     EILNKK"
BASE COUNT         2696 a         1261 c         1142 g         2819 t
ORIGIN      
        1 attagattgc cgaaacacga aactgaccga ttggttaaca tctgtttcaa aaaaatgagt
       61 tgaacaattc taaaataatg ttataataca ggtattgagg gctaaaggtc cacctatatt
      121 gatttgagct ttcggctcac tgacgtctgt attatacagg cgtctttttt tatacaattt
      181 tcacgggtag cccgcctacc cttattattt tttgccaatt ttgaggaggg aacgcatgaa
      241 aacacgttgt tacgatggta aaaaatggca atatgaattt aagtatgaag gaaaaagata
      301 ccgtaagaaa ggttttagaa caaagcgtga agctaattct gctggactag acaagttaaa
      361 tgagttaaga agtggtttta atatagataa ctatataact cttgaagaat acttcgaaaa
      421 ttggattaaa acgtataaac aacctgttgt taaagaaaat acctaccgtc attatagaaa
      481 tgcattacaa catatacaaa aacataaaat aggtaaaatg gagttatcaa agataaatag
      541 acaagtttat cagaaattca taaacgatta ttcaaaagaa cacgcaaaag aaactataag
      601 aaaaacaaac ggtgctattc ggtcagcttt agatgacgca ttatatgatg ggcttatttt
      661 taaaaatccc gcttataaag ttaattataa agccggaaaa cctacgaagt cagaacaaga
      721 aaaattcatc tcggtaactg aatatgaaat actaaaagat cacgtcagaa agaagagaac
      781 tcgttcatca ttagcgctat tcataatgat ttgtacgggt tgtcgtgtca gtggtgcaag
      841 aaatataaag attgagcata tcaaccaagt gaaaaacact atatttattg acgagcgaaa
      901 aaccgatact tcccctagat atatcagtat cgctaaatct gatatgaaac acattatgga
      961 cgtcataagt acatttgcaa ttagctatga tggttacatt ttcaaagaag ccggatctat
     1021 aattaacctt catgctatca ataatgcttt gaaatcagcc tgtagagtca ataatatacc
     1081 aattattaca tcgcacgcat taagacacac tcattgttct tatttactag caaaaggtgt
     1141 atctatacat tacatttcta aaagattagg tcataaaaat atagcaataa ctacatccgt
     1201 gtattctcat ttgttagaag aaaaatttaa tgaagaggac aaaaaaacaa ctaaaatttt
     1261 agaaagtatg taatttaggg acccattagg gactccaaac ccaataaata ctgttgttac
     1321 aaggtttcta tgtatccaaa ctgggggcaa tataaacgcg ctgatttaat cggacaatct
     1381 tcttatatta aaaataatga tgtcgtaata ttcaatgaag catttgataa tggtgcatca
     1441 gacaaattat taagtaatgt gaaaaaagaa tatccttatc aaacacctgt actcggccgt
     1501 tctcaatcag ggtgggacaa aactgaaggt agctactcat caactgttgc agaagatggt
     1561 ggcgtagcga ttgtaagtaa atatcctatt aaagaaaaaa tccagcatgt tttcaaaagc
     1621 ggttgtggat tcgataatga tagcaacaaa ggctttgttt atacaaaaat agagaaaaat
     1681 ggtaagaacg ttcacgttat cggtacacat acacaatctg aagattcacg ttgtggtgct
     1741 ggacatgatc gaaaaattag agctgaacaa atgaaagaaa tcagtgactt tgttaaaaag
     1801 aaaaatatcc ctaaagatga aacggtatat ataggtggtg accttaatgt taataaaggc
     1861 actccagagt tcaaagatat gcttaaaaac ttgaatgtaa atgatgttct atatgcaggt
     1921 cataatagca catgggaccc tcaatcaaat tcaattgcga aatataatta ccctaatggt
     1981 aaaccagaac atttagacta tatatttaca gataaagatc ataaacaacc aaaacaatta
     2041 gtcaatgaag ttgtgactga aaaacctaag ccatgggatg tatatgcgtt cccatattac
     2101 tacgtttaca atgatttttc agatcattat ccaatcaaag cctatagtaa atagtgctca
     2161 actaactaat aactcgcttc gttctaaaag gtcgaagcga gttatattgt taaaatttga
     2221 attgacttac attttaataa aatcatctta acaattttaa tttttcatta atacaagtct
     2281 ttactctaca ctcaaacaag attcatacac tgcacatcat aataaatcta tctattcaaa
     2341 tataaataaa agttacctac tacattctat gtagcaggca acttttatta cttatttctt
     2401 ttcattatca ttaagcactt ttacaaactt cacattatgt gtcttccaat caacttcata
     2461 taatgctgat aatttttctt cttttttatc tacatggttt tcaccagacc aatagcccca
     2521 gaaaccatga cgattccaat ctattttaaa ctcatccatt gatcttttat aatgaacaac
     2581 aaattttgat ttaccttcat cttttttatc atgtgacata acagctaaaa attctggatt
     2641 aaacccttca gaaacagtta caggcatctt attcttagga gtaaaattat ctttcgccca
     2701 taaatttcca tttcgtgtta aagaaaagat ttcactttta gttctattat cactatcatt
     2761 agttaattgt ctcgtatggt catgtcccat attatttatc aaatgtgctt ctactttcca
     2821 acctacacct ttatgtgacg tagattgatc aagtaatgta cgatacgatg gttgttgata
     2881 actaatcgtc tctgaataat tactctcttt tgtaatattt ccagttaacc ctccacgatt
     2941 aatcgaaaaa tctccacctg ttttataacc atacgtatat ttaacttctc ttgattcatc
     3001 ctgatttttt ggtgcaaagt cagtcacatt tgtattgttg ttgtcatcaa cattttgaat
     3061 tgaaactgaa taagatccag gccatcttaa tgtactattc caataaccat ttgggtcttg
     3121 aattcttaaa ccactaccta ttgttccttt cgctttaata aatactgttt ctttatcata
     3181 attaggttca gttaagaagt taaattgtaa gctttgagta atattttttt gactatcact
     3241 tgttgtagct gtacgtgtat acattttagt atcaccatca agattcttct cagaaacagc
     3301 tttaatttca gaattaattt ttgcataaga agtagctggt aatacagtga aagtagtcga
     3361 tagtgctaac gtacaaattg tgatattttt atatagttgt ttaatcattt gtaatccttc
     3421 ccttcgattt tatccttctc taaatgattt attatcagag tatttatcaa cgactttaac
     3481 tgttttattt ttccaatcaa cttcataagt gacaattaat ctttgacctt ctttattttt
     3541 ttctaaaatt ggaggtgcat aatgtattcc aggtctgttt ttcaaaatat cttgatttcg
     3601 tgtgtatgtt acttcaaatt gcgttttctc atttgacttt tcattagata aataagttaa
     3661 aaattctgga ttaaagccac ttcttactaa tgctgggtat ctatattttg aagcaaagct
     3721 tagttcaggg ttttcaacag tagcaattct cgtatttcta tagaataata attcatcatt
     3781 tctatttttc acttctccac catacttcaa gtcattcgca ataactgacc agtgtacatg
     3841 ccagttatta tttttaccgc tggcaattgt gtcataattt tgctgattat aactaatcgt
     3901 tttggagtag ctatttgatg aagttcgtcc aatacctttc gttgaatcga atttaccacc
     3961 tgagctatat gaaaatgtac tgtctacttt cgcagttgaa attttatttt tcggcaattg
     4021 gtctaatatt tcagttttag gatttctttt tacttgaaaa tctacatggt actcacttgg
     4081 atactttaac caatttgaat ttttttcttc tttatgagat tcaaacttta aatttgaatg
     4141 aattgagcct tgttttttaa caagtaatac attcttgtca taagttggat catcgataaa
     4201 gtcgaattgt aaattttgga gtatatttgt tttctcatca tatactgttt cagttcgttt
     4261 tgtgatttta ccgtttttcc caatatcatc cggtgctgtt gaatttttat ctttattagt
     4321 aacattacgt ttgtcttttt gttgagactt atcaacatgt tctttcttat tttggtcttg
     4381 agagtcttta tgagctgaat ttgcttgagt cgttgctgct gataacaata aaattgcaca
     4441 tgataatgat gacgctatta aaacacgttt tttatttttc attgttataa ccttctttcg
     4501 tatgattgat atttgttgat atgtatcgac atgtgaataa tatcacaaaa acagagaata
     4561 tatatttaac tatttattaa atgattttgt taatattatt aaatacttta tcctctttaa
     4621 aaataatgtg tgtacaaagt cattaattta gcaaatattt ttatttagta gttaataacc
     4681 atcgatttga aatttatata taattattag ctaaataata tcctgcatct ttctgataca
     4741 atttactata aaattgagca tacccgatat cagtgttaat aagatcgttg atactagaca
     4801 gttaatttca tagaacgaaa tcaaataaca cactactttc tgcattttaa attatgttta
     4861 agaataaaaa ttatgtttat ataaatatat atactacttt gaaaggtgtg agcttaatga
     4921 caacttttag tgaaaaagaa aaaattcaat tactagcaga tattgttgaa ctacaaactg
     4981 aaaataataa tgaaatagac gtttgtaatt atttaacaga tttattcgac aagtatgata
     5041 ttaaatctga aattttgaaa gttaatgaac accgcgccaa tatcgttgca gaaatcggta
     5101 acggctcacc tatactcgca ttgagtggtc atatggatgt tgttgatgca ggaaatcaag
     5161 ataattggac ttatccccct tttcaactca cagaaaaagc tggcaaatta tacggtcgag
     5221 gcacaacgga tatgaaaggc ggtctgatgg ctttggtcat cacactcatt gaattaaaag
     5281 aacaaaatca attgcctcaa ggaacgataa gattactagc tactgctggc gaagagaaag
     5341 aacaagaagg tgccaaatta ttggctgata aaggctattt agacgatgta gatggcttaa
     5401 ttattgctga accaactgga tctggaattt attatgcaca taaagggtct atgtcatgta
     5461 aagtaactgc aactggcaaa gctgtccata gctcagttcc atttattggt gacaatgcaa
     5521 ttgatacact gcttgaattt tataatcaat ttaaagaaaa atattcagag cttaaaaaac
     5581 atgatactaa acatgaatta gatgttgcac ccatgttcaa atcattgata ggaaaagaaa
     5641 tttctgaaga ggatgcaaat tatgcatctg gtcttacagc tgtatgttcg attataaatg
     5701 gcggcaaaca atttaactct gtaccagatg aagcttcact tgaatttaac gtaagaccag
     5761 ttcctgagta tgataacgac tttatagaat cgtttttcca aaatatcatt aatgatgtgg
     5821 atagcaataa gctttcactc gatattccaa gcaatcaccg acctgtaaca agcgataaaa
     5881 atagcaaatt aattactacg attaaagatg tagcttctag ttatgtagaa caagacgata
     5941 tatttgtttc ggcgcttgta ggtgcaacag atgcctctag tttcttaggt gataataagg
     6001 acaatgttga tttagccatt tttggaccag gtaatccatt aatggcacat caaatcgatg
     6061 aatatattga aaaagatatg tatctgaaat atattgatat ttttaaagaa gcttccattc
     6121 aatatttaaa agagaaataa gaacgatgct gtcagctgcc ctattcgcgt gctggcagtt
     6181 ttttattgcg aattttaagt tgatagaaat aacgatgaat attgtttgaa tgtaaatatt
     6241 caaatatcaa caagcacatt ttcattgatt aagtgatgta aaactgaaat tattgtgctg
     6301 atttgtcata catatattga ctaatggtca tataaaaaga tagcttctaa tagttacata
     6361 aaactcgtaa aaaccttctc gtaaaaggaa tgggacataa atccctagaa aacagcagct
     6421 caacgagctg taaattatca aatacatttg aaaaaagctg atttctatca aataatttat
     6481 agaaatcagc ttttttacat tgtctaagaa cttaatgtct ctgtctcttt ggaactttat
     6541 atttcataag tactatttta ttgtttaacc tttttttaaa taaggtataa atctacaata
     6601 tccaaataag ccccatcgat tcttataata gtcaaaataa ttatattcat gtattactct
     6661 tattgtattt ggtaaaattt cattcaattt ttttaaatca ttgtaaccat atttaaaaac
     6721 aggttgtgag gataactttg ataccgattg atgtctcttt gtattattcg caattgtttt
     6781 tgagcaaaat tcaatcgcaa atgtcaaatt atgatctccc atctttttga taatagtgtc
     6841 caataattga gtcattacac tctcatcaaa atacatcaat acaccttcaa taataaacaa
     6901 tatatctgac ttactattaa aaaatttata atttttgaca tcatcaatcc aactgtaatc
     6961 tagcatagat ttagatatca tcttataact attactttct ttaaaaaatg tttttcgtat
     7021 ttctattgat tctggtacat ctaaatctat ccatgatatt ttttctttat taaatctttg
     7081 aaaccttgta tctaagccac aacctatatt gaccacgatt aaatctttat tatccttgat
     7141 aagcctctta gtaacacaat ctattatcac agtacgtata ctaattccaa tttgagacat
     7201 gtcatcacat gtaacatttt tgtacatatc atctaaacca tcaaatattt ttttagatag
     7261 tgcgtctttt attattggtt tttcgttttc gtactctttt gctcgagcta tcaaaggaat
     7321 taacattgat tcgggaattc caaccagttg cttcatttaa tcactcctta aaaacattag
     7381 taccaatgca taagaaaatt tttacaactt atttctatta ttttttattt aaaatttctt
     7441 tttttaattt acttctcaaa tgttctaatg taataggatc tgtgaaccaa taatcttctg
     7501 ctttatatga aattacatgt ccttttttta cagcttcaat attctgccat gtatgtgttt
     7561 tttcaaaatc aaattttccg tacgaaggtt tacttaaaaa tatataatca ccagcatatt
     7621 tactaatatt ttcttttgaa atggaagcat agcctttttt atcttcttgt aatttatctt
     7681 tatattgctt tgtcattggc ataccgaatg catcatgaac aatatccaaa ccacgacccc
     7741 atgtagagtt atatatgtat atttgctttt catctggttc aaacacagat gctgttgctt
     7801 gaccaatttt actttgaatt tcttttttat cttttctagt tttatcgtcc cactcttcaa
     7861 tccatttttt agctttatct tcattattag tcaacttgcc tatttctttt aatatttc
//
LOCUS       DAHHYJ010000122         7672 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000122 DAHHYJ010000000
VERSION     DAHHYJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.122"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(26..649)
                     /gene="lexA"
                     /locus_tag="NBK22_002105"
     CDS             complement(26..649)
                     /gene="lexA"
                     /locus_tag="NBK22_002105"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCD2415283.1"
                     /translation="MRELTKRQSEIYNYIKQVVQMKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTNDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            792..1025
                     /locus_tag="NBK22_002106"
     CDS             792..1025
                     /locus_tag="NBK22_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415284.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            1161..1400
                     /locus_tag="NBK22_002107"
     CDS             1161..1400
                     /locus_tag="NBK22_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCD2415285.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            1521..3509
                     /gene="tkt"
                     /locus_tag="NBK22_002108"
     CDS             1521..3509
                     /gene="tkt"
                     /locus_tag="NBK22_002108"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD2415286.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDNAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEDERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYLELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TVGKVIAIDGFGASAPGDLVVEKYGFTKENILNQVMSL"
     gene            3787..4029
                     /locus_tag="NBK22_002109"
     CDS             3787..4029
                     /locus_tag="NBK22_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCD2415287.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            4016..4114
                     /locus_tag="NBK22_002110"
     CDS             4016..4114
                     /locus_tag="NBK22_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415288.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYDY"
     gene            4208..4675
                     /locus_tag="NBK22_002111"
     CDS             4208..4675
                     /locus_tag="NBK22_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCD2415289.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            4799..5920
                     /locus_tag="NBK22_002112"
     CDS             4799..5920
                     /locus_tag="NBK22_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD2415290.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIMISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISG
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            5924..>7672
                     /locus_tag="NBK22_002113"
     CDS             5924..>7672
                     /locus_tag="NBK22_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCD2415291.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIESLRTEGEQLGDELNNLIKGLEKVEDSIGNNESDYEKIIELNNAITNINNEINII
                     KENEKAKAELDKLLGSKQELENQINEETTIMKNLEIKLDHYDKSKLDLNDKESFISEI
                     KSAVKIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHNSEIK
                     FVNEKISNINIKTQSDF"
BASE COUNT         2899 a         1016 c         1359 g         2398 t
ORIGIN      
        1 cgataatata taacggttta aaatattaca tttcgcggta caaaccaatt actttcccaa
       61 ttacagcaac attgtcgagg taaattggct ccattgtact attttcaggt tgtaatcgat
      121 aacgattttt ttctttatag aagcgtttga cagttgcttc atcttcctca gtcatagcaa
      181 caataatgtc tccattttct gctatggttt gactgcgaac aattacttta tctccgtcta
      241 atataccagc ctcaatcata ctgtcgccta cgacgtttaa tatgaatatg tcgctattgt
      301 gtgtcgatgt taagtgttca ggtaatggaa aatattcttc aatattttct actgcggtaa
      361 taggaacacc tgctgtgact ttaccaataa ctggcacatg aatcgtttct tccatattaa
      421 tattatcatt tgtttgatca cttacaattt ctatagcacg tggtttcgtc ggatctcttc
      481 ttatatagcc tttttcttca agacgtgaaa ggtgaccatg aacagttgaa ctggatgcta
      541 agccaactgc ttcaccaatt tcgcgaacac taggcggata acctttcatt tgaacaactt
      601 gtttaatata gttatatatt tcgctttgtc gttttgttaa ttctctcata tataggcact
      661 ccctaaatta atttatatac agtatatcat taattcacaa ccaaaacaaa catttgttcg
      721 ttaaaatatt gacagagaac ataagttctg ataaattact aatacaaaca aatgttctag
      781 gagtggaaat gatgttttac aataaatata aaaacgtatc aacatatatc atcatatttt
      841 tagtttcaag tgcagccttt gcaatattct tgttaagtgc gaacattagt gctcactcgg
      901 aacaagtgta cgaaatgact gaccatcaaa ttaagaacaa tacgataaat aaagcatacg
      961 aacataaaga ccctacaaac aatagcgaac aaagagatgg gaaagtgttc gctttaataa
     1021 attgatacat tgtcacaacg ttattttgcc tatttttgcg aaatagcgtt ttttattact
     1081 tttttactga tcttaaattt gttatatttt gttaaagtat tataatgatt gaataaacaa
     1141 attgaaggta ggttttttaa ttgagtaatt ctgatttgaa tatcgaaaga attaacgagt
     1201 tagctaaaaa gaaaaaagaa gtaggattaa ctcaagaaga agcaaaggag caaacagcct
     1261 taagaaaagc ttatcttgag agttttagaa aagggtttaa acaacaaatt gaaaatacta
     1321 aagtaattga tccagaaggt aatgatgtaa cacctgaaaa aattaaagag atacaacaaa
     1381 aaagagataa taaaaattaa atcacaaatc tgtaaagaat tttctgacat tataacttga
     1441 aataagtatt ttacttatct ttttatttta aaataagtta taatgtattt gataaaattg
     1501 aagaagggaa gatacacaag atgtttaatg aaaaagatca attagctgtt gatacgctgc
     1561 gtgcactaag tatcgacaca atcgaaaaag cgaattctgg tcatccagga ttacctatgg
     1621 gagctgcccc aatggcttac actttgtgga cacgtcatct gaattttaat ccacaatcta
     1681 aagattactt caatagagac cgtttcgtat tatctgcagg gcatggttca gcattattgt
     1741 atagcttgtt acatgtttct ggtagtttag aattagaaga attaaagcaa tttagacaat
     1801 ggggttctaa aacaccaggt catcctgaat acagacatac agatggtgta gaagttacta
     1861 ccggaccact tggacaaggt tttgctatgt cagtaggatt agctttagca gaagatcacc
     1921 tagcagggaa atttaataaa gaaggatata atgttgtaga tcattacaca tatgtattag
     1981 cttctgacgg tgatttaatg gaaggtattt cgcatgaagc agcttcattt gctggacata
     2041 ataaattaag taaattagtt gttttatacg attcaaatga tatttcatta gatggcgaat
     2101 taaacaaagc tttttctgaa aacacaaaag ctcgttttga agcatatggt tggaattact
     2161 tactagttaa agatggtaat gatttagaag aaattgataa tgcgattact actgctaaat
     2221 ctcaagaagg accaacaatt atcgaagtta aaacaacaat cggatttggt tcaccgaata
     2281 aagcaggaac taatggtgtt catggggcgc ctttaggtga agatgaaaga aaattaacat
     2341 ttgaaaatta cggtttagat cctgaaaaac gttttaatgt ttcagaagaa gtatacgaaa
     2401 ttttccaaaa tactatgtta aaacgtgcta atgaagatga atctcaatgg aattcattat
     2461 tagaaaaata tgcagaaaca tatcttgaat tagcagaaga atttaaatta gcaattagtg
     2521 gtaaattgcc taaaaattat aaggatgaat taccacgttt tgaacttggt cataatggtg
     2581 catctcgtgc tgattctggt actgttattc aagcaatcag taaaactgtc ccttcattct
     2641 ttggtggatc agcagacctt gctggttcaa acaaatccaa tgtaaatgat gcaactgatt
     2701 atagttctga aacacctgaa ggtaaaaatg tgtggtttgg tgtacgtgaa tttgctatgg
     2761 gtgctgctgt aaatggtatg gctgcacatg gaggtttaca tccatatggt gcaacattct
     2821 tcgtatttag tgattattta aaaccagcgt tacgtttatc atcaattatg ggattaaatg
     2881 caacgttcat cttcacacat gattcaattg cagtaggtga agatggtcct actcatgaac
     2941 caattgagca attagctgga ttaagagcca ttccaaatat gaatgttatc cgtcctgctg
     3001 atggtaatga aacaagagta gcatgggaag ttgccttaga atctgaatct acacctactt
     3061 cattagtatt gacacgtcaa aacttaccgg tattagatgt accagaagat gtagttgaag
     3121 aaggcgttcg aaaaggtgcc tatacagttt atggctctga agagacacca gaattcctat
     3181 tattagcttc aggttcagaa gttagtcttg cagttgaagc tgctaaagat cttgaaaaac
     3241 aaggtaaatc agtacgtgtt gtttcaatgc ctaactggaa tgcatttgaa caacaatctg
     3301 aagaatataa agaatcagtt attccatcaa gcgtaacaaa acgtgttgcg attgaaatgg
     3361 cttcaccgct tggatggcat aaatatgtag gtactgtagg taaagttatt gctattgacg
     3421 gctttggcgc aagtgcacct ggcgatttag tagttgaaaa atatggattt actaaagaaa
     3481 atatcttaaa ccaagttatg agcttataag aataatttat aaagcgagta tgtttagaag
     3541 tctaggatgc ataatcttag gcttctttta aagtgttgaa atttagagta tagcacttaa
     3601 actacatcat aagtgataag ttatgaaagt atactatttc agattaatct ttaaaagctc
     3661 tgttataaca gcatgatttt tgatattatt tttagtatcg atattaaaat acttgaataa
     3721 actagttctt gaaataatgt gatgaattta gtaaaattca gtaggataaa gaaagcgggt
     3781 gaaacaatgg caacttggtt agcaattatt tttatagtag ctgcattaat tttaggttta
     3841 attggaggtt tccttttagc tagaaaatat atgatggact acttgaagaa aaacccacca
     3901 atcaacgaag aaatgcttcg tatgatgatg atgcaaatgg gtcaaaaacc ttctcagaag
     3961 aaaattaatc aaatgatgac gatgatgaat aaaaatatgg atcaaaatat gaagagtgcg
     4021 aaaaagtaaa ttcgcaattg atagaggcta ttttccagat atggaaatgg cctcttttta
     4081 taatcaaatt aataaaaaca aatatgatta ttaaaattaa agttaacaaa atgacgaata
     4141 gactgagaaa tgctataatt cattttgtat gatttacaga gagtttattt aacgagaagg
     4201 tgtctgcgtg ctctatttaa tattttcaat cattgtagct ttatttatgg gaactatagt
     4261 tatagttatt cgtatgaaag ctcaaaatta tccggtaaat gagaaaaaaa tagttttgcc
     4321 accgtttttt atggcgaccg gtgcattgat gtacgtcgtt ccatatttta ggctaacagg
     4381 atcggaaatg ctagaagcct ttataattgg tttgcttttt tctacagttc taatttggac
     4441 ttctcgattt gaagtcaaag gtacagaaat ttatatgaaa cgatctaaag catttccagt
     4501 tattttgatt tcattactta tcattcgtac tgtgatgaaa atattcatta gtaatgaaat
     4561 agatcctgga gaattaggcg gcatgttctt tttattagca ttctgtatga ttgttccttg
     4621 gagagcagca atgctatata aatacaaaaa actaaagaaa acattaatca attaattact
     4681 tttaaaacca cttgtgatcg acttctaaat cagtcgatga gtggttttaa ttttacttga
     4741 aaaggtgaaa tgattatatt aaaataaaag cgaacaaatg ttctatatgg aggcagtaat
     4801 gaaaattata catacagcag actggcactt agggaaaata ttaaatggca aacagctttt
     4861 agaagatcaa gcgtatattt tagatatgtt cgtagaaaaa atgaaagaag aagaacccga
     4921 tatcattgtg atagctggag atttatatga cacaacatat ccaagtaaag atgcaatcat
     4981 gttattagaa caagcgattg gaaagttaaa tttagaactg cgtataccaa taattatgat
     5041 tagtggaaat cacgatggta aagagaggtt aaactatggg gcgagttggt ttgaacataa
     5101 tcagttattt ataagaacag attttacatc gattaattca ccaatagaga taaatggggt
     5161 taatttttat acactccctt atgctactgt gagcgaaatg aaacactact ttgaagatga
     5221 caccattgaa acacatcaac agggaattac gcgctgtatt gaaacaatag caccggaaat
     5281 tgatgaagat gccgtcaata ttttaattag tcatctgact gttcaaggtg gaaagacatc
     5341 tgattctgaa agaccattaa ctattggaac ggttgaatca gttcagaaag gtgtttttga
     5401 tatatttgat tatgtcatgc taggtcactt gcatcatcca tttagtatag aagacgacaa
     5461 aattaaatat agtggctcct tattgcagta ttcattttcg gaagcgggtc aagctaaagg
     5521 gtatagacgt gtaacaatta atgatggcat tattaacgat gtatttattc ctcttaagcc
     5581 acttagacaa ttggaaatta tctcaggcga atataatgat gttattaatg aaaaagttca
     5641 tgtgaaaaat aaagataatt atttacattt taaacttaaa aatatgtctc atattactga
     5701 tccaatgatg agtttaaaac aaatttatcc taatacttta gcgctgacga atgaaacttt
     5761 taattacaat gaagaaaata atgctataga aataagtgga aaagatgaca tgtcaattat
     5821 cgaaatgttt tataaacata taactgataa agaattatcg gatatccaat ctaaaaagat
     5881 aaaaaatatt ttagaaaacg aattgagaaa ggaggattaa tgaatgaaac cattacattt
     5941 aaagttgaat aatttcggcc cctttttaaa agaagaaatt gatttttcta aaattgataa
     6001 taatgaattg tttttaataa gtggtaagac tggatcgggt aaaacaatga tttttgatgc
     6061 aatgacttat gccttgtttg gtaaagcatc aactgaacaa agagaagaaa atgatttgag
     6121 aagtcatttc gctgatggta aacagccgat gtcagtaaca tttgaatttc aattaaatca
     6181 tcgaatttat aaagtgcata gacaaggccc ttatatcaaa gaaggtaata caacaaaaac
     6241 gaacgctaaa tttgatgtat ttgagatggt ggatggcaag tatgaaatta gagaaagtaa
     6301 agtaatttca ggtacccaat tcattattga attattagga gtaaatgcag atcaattccg
     6361 acaattgttt attttgcctc aaggtgaatt caaacgcttt ttaatatcaa acagtcgtga
     6421 aaagcaaggg atattaagaa cactgtttga cagtgaaaaa tttgaagcta tacgagaaat
     6481 attaaaagaa gaagtaaaaa aagaaaaagc tcaaatcgag aatagatatc aacaaattga
     6541 ccttttatgg caagaaattg aatcatttga tgatgacaat ataaaaggct tattagaggt
     6601 tgccactcaa cagatagaca aattgattga aaatatacca cttttacaag ctaggtcgaa
     6661 agaaatacta gcatctgtaa atgaaagtaa agaaactgct attaaagaat ttgaaataat
     6721 agaaaagaaa acattagaaa ataatatatt aaaagataat attaatcaac tcaacaaaaa
     6781 taaaattgat ttcgttcaat tgaaagaaca acaacctgaa atagagggaa ttgaagctaa
     6841 gttaaagttg ttacaagata ttacaaacct attgaattat attgaaaata gagaaaaaat
     6901 tgaaactaaa attgctaata gcaaaaaaga tatttctaaa accaataata aaatattgaa
     6961 tcttgattgt gataagcgaa acatagacaa agagaaaaaa atgttagaag aaaacggaga
     7021 tttaattgaa agtaaaatct cttttattga taaaactaga gtattattta acgatattaa
     7081 taagtatcaa caaagttatc tcaatattga aagcttgaga actgagggtg aacaattagg
     7141 tgatgaatta aataatctaa ttaaaggttt agaaaaggtc gaagattcaa taggtaataa
     7201 cgaaagtgat tacgagaaaa ttatcgaact aaataatgcg ataactaaca taaataatga
     7261 aattaatata attaaagaaa atgaaaaagc taaagctgaa ttagataaac tattaggtag
     7321 taagcaagag ttagagaatc aaattaatga agaaacaact ataatgaaga atctcgaaat
     7381 aaaattagat cactacgata aatcaaaatt ggacttaaat gataaagaaa gctttataag
     7441 tgaaattaaa tctgctgtaa agattggaga tcaatgtccg atatgtggta atgaaattca
     7501 ggatttaggg catcatattg attttgacag tattgctaaa cgtcaaaatg aaattaaaga
     7561 aattgaagca aatatccacg caataaaatc gaatattgct gtgcataatt ctgaaattaa
     7621 atttgttaat gaaaaaatat cgaatattaa tattaaaacg caaagtgatt tt
//
LOCUS       DAHHYJ010000123         7624 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000123 DAHHYJ010000000
VERSION     DAHHYJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.123"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(95..403)
                     /locus_tag="NBK22_002114"
     CDS             complement(95..403)
                     /locus_tag="NBK22_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCD2415292.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(418..846)
                     /gene="ruvX"
                     /locus_tag="NBK22_002115"
     CDS             complement(418..846)
                     /gene="ruvX"
                     /locus_tag="NBK22_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCD2415293.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(850..1110)
                     /locus_tag="NBK22_002116"
     CDS             complement(850..1110)
                     /locus_tag="NBK22_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCD2415294.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(1173..3803)
                     /gene="alaS"
                     /locus_tag="NBK22_002117"
     CDS             complement(1173..3803)
                     /gene="alaS"
                     /locus_tag="NBK22_002117"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCD2415295.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQMKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(4146..6623)
                     /locus_tag="NBK22_002118"
     CDS             complement(4146..6623)
                     /locus_tag="NBK22_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCD2415296.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNTLGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDRNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMSSNDSGEVTSDSTKTDIN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(6625..7293)
                     /locus_tag="NBK22_002119"
     CDS             complement(6625..7293)
                     /locus_tag="NBK22_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD2415297.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTNM
                     KEEE"
BASE COUNT         2282 a         1492 c         1031 g         2819 t
ORIGIN      
        1 aaaattataa gcgttaatta tttatacatt gcttaactta aaaaaatact cttgcctccc
       61 catcttaagg ttagcaagag taaaatcttt ttaattattc ttccatttca gtatttacaa
      121 cttcttcaat catgtcccat tcttcatcag tttcgattgg tactaactta ccaccgtcac
      181 ctgactcatc tggttcattg atcattggta caagctcaat catatcgtct tcatctgatt
      241 gagcaccttc ttcagctaag ataacatact cttttttgaa ttcaggatga taaaattcta
      301 aaacttttcg gtataaaact tcatttccct cttcatcgaa taaagttaat aattcttctt
      361 cgttattaat ttctagttgt gaatcatgat tatgttcagt catagtaaaa tctcctttta
      421 atgtagtgaa tctaaatagc cttgtaaaat aaataccgct gccattttat caatcacttg
      481 ttttcttttt tgtcttgaaa catctgcttc taataatgat cgttcagcag ccattgtgct
      541 taatctttca tcccacatca caatctcaat agaaggataa gcttctaata atttttcttt
      601 atatgttaac gaagcttcgc ctcgaaatcc tattgaatta ttcatgtttt taggtagtcc
      661 tattacgact gtacccacat tatgtttttt aataatgtct actaattggt caatacctaa
      721 ttcattattt tcttcattga ttcggagtgt gtctaatcct tgtgccgtcc aacccattat
      781 atcactaatt gcaattccta ccgttctact accgacatcg agtcctaaaa ttttatgttg
      841 taacataaat tatttatttt gctcttttaa atagtaagaa acaagctctt ccataataac
      901 atctctatca atatgacgaa tttgatttct tgcttcattt tggcgtggaa tatacgcagg
      961 gtcacctgat aataaataac ctacaatttg gtttacggca ttatatcctc gttcatctaa
     1021 tgttcgataa acattattta aaacatctct tacatcttgc gttggaagtt cttcatagtc
     1081 gaatttcatt gttttatcaa agttttccat ttgcgacact cctttaatta caaatataac
     1141 tcactatcat catacaatat tatggcttta aattatagat ttttaatgta atctttaata
     1201 aagcttaatg attttgagat attttcaggt tgtgtaccgc caccttgagc catatctgga
     1261 cgaccgccac ctttaccacc aacgattggt gccatttgtt tgataagatc accggcttta
     1321 acgttatttg ttaaagattt agggacagtt gcaaccatcg atactttatc atcaacatta
     1381 cttgcaagaa tgataattgt atcttgcagt ttagatttaa aatcgtccat tgtcgagcga
     1441 attgctttcg catttggtac atccacttca gtaaccaata ctttatagcc attgatttct
     1501 tcaacttgat cttcaatatt acccatttta agtgatgtga tttctttgtc acgttgctct
     1561 aattgtttta ataatgcttt ttcttcatct tgtaattgtg ttaacttatc gactacttga
     1621 tcatcagatt tcactttcat ctgtgatttc atcgtattaa atttctcttg aatatcttct
     1681 aaatataaga aagctgcttt acctgttaat gcttcaatac gacgcacacc agctcctgta
     1741 cctgactcac ttactatttt gaataagcca atttcagaag tattgcggac atgaatacca
     1801 ccacataatt caattgaaaa tggtgccata tttactacac gcacaacatc accatatttt
     1861 tcaccgaata atgccattgc gcccatttct ttagctgaag caatatccat ttcttgaatg
     1921 ttaacgtcaa tacctttcca aatttcttca tttactaagc gttcaacttg atcaatttca
     1981 tcattagtca ttggaccaaa atgagagaaa tcaaaacgta aacgatctgc ttctactagt
     2041 gaaccagctt ggttaacatg atcacccagt actgatttca acgctgcatg taataaatgt
     2101 gttgcactat ggttcttttg aatgtcacgt cgatcatttt ggttcacttc agcagacact
     2161 gtagcgccaa catttacttg gccaaattgt actactcctt tatgcaagtt ttgaccattt
     2221 ggtgctttgg ttacttcact aacagcaatt tcaaaattgt cattataaac aatacctgta
     2281 tccgcaactt gtccaccact gactgcataa aatggtgttt ccgttaacat gaagtatact
     2341 gtttcacccg cttcaacttg tgaaacttct tcaccattat atatcaagtg tgttagtgtt
     2401 gtttgagctg tcgcagtatc ataaccaaca aaagtacttg cagatgtaat atttttcaat
     2461 acttcacttt gaacttgcat tgattgagaa ttttgacgtg cttgacgtgc acgatcacgt
     2521 tgttgttgca tttctgactc gaatgttgtc atatcaactt tcaatcctgc ttgaactgct
     2581 atttcttcag ttaattcaat tgggaacccg tacgtatcat acaatttaaa tgcatctttc
     2641 ccattaattt catttgttgt cgctttagct tttttaatta attcatttaa aatcgctaaa
     2701 ccatcttcta atgtttcatg gaatcgttct tcttcagact ttataacacg cttaatgaaa
     2761 tctgcttttt ccttaacatt tggataatat ggttccataa tgtctgcaac aatatcaaca
     2821 agtttgtaca taaatggctc attgattcct aacgtttgac taaaacgaac ggcacgacgt
     2881 aacaatcgac gtaatacata tcctctacct tcattggcag gtaatgcacc atcagaaatt
     2941 gcaaatgcaa tcgtacgaat gtggtcagca attactttaa atgccacatc ttgttcgttg
     3001 tttactaaat attgtttacc tgatactttt tcgatttcat tcattatagg cataaataaa
     3061 tctgtttcat agttagtacg tacattttga gaaactgagg ccatacgctc aagccccatg
     3121 ccagtatcaa tgtttttatt aggtaatggt gtgtaactat gatctttatt atgattgaat
     3181 tcactaaata ctaagttcca tacttcaaga tagcgttcat tttctccacc tggatacatt
     3241 tcttctgccg gatcgtcttg tccatatgct tctccgcgat catagaaaat ctcagtgttt
     3301 ggtcctgaag gcccttcacc aatatcccag aagttacctt caatgcgaat aatacgactt
     3361 tcttcaagcc caatatcttt atgccaaatg ttgtatgctt ccatatcttc cggatgaatc
     3421 gtaacgtaca atttatctgg ctccatcccc atccatttat cactcgttaa aaattcccaa
     3481 gcaaattcaa tcgcttcttg tttaaaataa tcaccaattg agaagttacc taacatttca
     3541 aagaatgtat ggtgacgcgc tgtgaaacca acattttcaa tatcatttgt acgaatagct
     3601 ttttgagagt ttacaattct tggcttttta ggtgtttcac gtccatcaaa atatttcttt
     3661 aatgttgcta cacctgaatt aatccataat aatgtatcat catcaattgg cactaatggt
     3721 gcagaaggtt caaccatatg tcctttttca acaaagaaat ctagatattt ttgtctaatt
     3781 tcactcgctt ttaacttttt catcttttac acatcctatt tactgttttt aaattaccat
     3841 tccataaaaa ttgatgacac agatagtcga tttgaaaaac tagtataaat caatatcatt
     3901 ttttattatt aaaaaataaa aaacgcccat cctcaaaagg gacgaacgtt atcgcggtac
     3961 caccctagtt ataaatgcaa ttcaacacat ttatcacttt aaatcgacta tacagttgtg
     4021 cataaagtag cgttcactaa tgtttgttgt acttttcacc aaccagtaca tctctgataa
     4081 acaaatcatt aactactcat ctttatacga atttaattct attttagtta catttacgct
     4141 tgttgtcaac gttctataaa gtcatacggc gtgatttctc ccatattaat cattgggtca
     4201 attttaaaca ttgtagcttc cgttaataca tttatatctg tttttgttga atcagacgta
     4261 acttctccac tatcattcga tgacattggc gcttctactt gatcatctat tgtcgtttgt
     4321 gaagctcctg tatcattagt tgctgtgttt tccagcattt cttcatcttc tgaattaaaa
     4381 taatttttca acaatgtaca taattgtgtt aaacgcgctt gaccatttgt ttttaatcca
     4441 atatcaaatg cttctggatc accaagtaga actaaactcg ttttcgctct agttaaacca
     4501 gtatataata tcggtctttg taacattcta aaatactgtt taacaatagg catgataaca
     4561 ataggaaatt ctgaaccttg tgatttatgg attgatgtac aataagcatg tgttaattcc
     4621 atcatatctt gtttcgtaaa tgtaatttca ttaccttcaa aatccacaac aagtacatct
     4681 ttattaaggg cattttcttt cgcccaaaaa ataccaacaa taactcctat gtcaccattg
     4741 aatatgttat catttggcct attaacaagt tgtaatactt tgtcaccttt tctaaagact
     4801 acatcaccaa actcaatttc tcgtgtgtct ttctttttag ggtttaaaat atcttgtaaa
     4861 acttgattta aacgtttaat accggcattt cctttataca ttggtgcaag cacttgaata
     4921 tcagccatag tatacccttt attaacagca ctagtaacta ccttctcaac aactgttggt
     4981 atttggtttg cctgacagtt aataaaactt ctatcatgaa aacgctgtgt aatatcaatt
     5041 ttctgaccca atttcattcg atgtgctaat tctataatgc ttgaaccatc ttgttgacga
     5101 tatacttcag tcagattaac tcgtggtata gctttcgatt caattaaatc tttaaatact
     5161 tgaccaggac ctacagaagg caattggtcc tcatcaccta caaatatcaa ttgtgcatct
     5221 aaaggaactg cacttaaaaa ttggtgaaac aaccaagtat ctaccataga catctcatca
     5281 atgattatga gtcgtgcgtt tatttcattt tctaatatat cctctggctt tgtgtcttga
     5341 ttccaaccta ttaaacgatg aatcgtcatt gcttctaatc cagttgactc ttgtagtctc
     5401 ttagacgctc ttcctgttgg cgctgctaat acaactggat aatcatcatt gacataatca
     5461 tcataatcta atgataagcc atgaatctca gcatataatt ctacaatacc tttaattact
     5521 gtcgtctttc ctgttccagg tccaccggtt aatagcatca ccttagaatt gatagccgtt
     5581 tgcaaagctt ctttttgtga agctgcatag ttcacttgat tcgcatcttc tatttcacca
     5641 atatgcattt gtaaatctga ctgttcaatt tccgtaagtt tatttgtatg cgtctttatt
     5701 ctgaataagt tttgaacact tttgatttca gaataataca aacttggaat tgcaacttgt
     5761 tcattgtcaa taattagtcg tttttcctca tttaagtatt gcaacatttc gtctaatttt
     5821 tcaggttcga tgacctcttc atcttgataa tttaatacat caaccgttaa atctataaca
     5881 acattgatag gcaaatatgt atgtccctgt ttaatacatt cttcttctaa cgtatagagc
     5941 aacgcagctt ttaatcgttc attatcgtta taagcgatac caatatttct agcaagttga
     6001 tctgctttat taaaaccaat acctttaata tcataaatca attgatatgg atttcgatct
     6061 aaaatagtca gtgtatcgcc gagataaaac tgataaattg ccattgaaag tttaggacca
     6121 aaccctaaat catgtaaacg aatcattatt ttttcagatt cttgatttgc tgaaatttgt
     6181 tctgcaattt gtttctgttt ctttttagat aatcccgaaa ctttttctag cactgaatgg
     6241 tcatctaata tatcatttat cgcattgtca cctaatgtat taacaatatt ttgagctgtc
     6301 tttttaccta cacctttaaa caaatcacta gataaataac ttataattgc ttctttcgtt
     6361 tgtggcattt ctttttcaaa agtctctgct tttaattgtt taccataacg tggatgatca
     6421 acaacttgcc ctttaaatgt gtagacatcg ccttcaacaa tattcggaag aaaccctaca
     6481 acagttggca ttgtatcaaa gtcttcattt gtttcaatag tatctacttt aagcactgta
     6541 taaaaattat cactgttttg aaacaatatc gcttcaacag tacctttgat cattgaataa
     6601 tcaaatagtg tagggtctga catgttactc ctcctctttc atattagtga atgttttcag
     6661 cgcatgctga cttaataaat gtttagggtc gatagtcaca gcttctttaa aatgagttat
     6721 tgcttcatca atatcttcat ttttcataaa taacgctaag cccaaattgt atcttgcatc
     6781 aacatgattt ttatcaatcg ttaatacatg tttaagttga gttatggctt cattaaacat
     6841 ttctaattga cataatacaa gaccatattg aaattgaact tctgcatctt tgtctttatc
     6901 tagttccgca gcagtcatta aatacggcaa tgccagctta aatgattcta actgattaaa
     6961 cgccataccg atcatataat tacaatcaac ttgttcaatc tctgtttgta atgcttgttg
     7021 atataattta atagcttctt gataacgttg ctgattataa tatacatttg ctagattata
     7081 aaatacgacg ccattcttcg gatctattgt taaagctttt tggaaaaaac gctctgcctt
     7141 ttcaatctca ttcgcatcag caagtacgat accagcatta atataatttt caataattgt
     7201 aggatcttct tcgatatttc cgaacaatgc ttgtaacgct tcttctattt ttccattttg
     7261 tatgtattga taaattgttt gttgatctat catttacgaa cctcatttct catcaattat
     7321 aacatcttga taaattgtat gtctcgaatc acttaacaac gaataaaata taatctaata
     7381 tcattttcat tcatgaaaaa gcgggaatgg aatagaaatg ctaaagaacc attaacggtt
     7441 tattatgtaa tggttcttcc acattagcca ccactattat gtacttaaaa ataggaatac
     7501 ataattagat tcatgcatag ggagtgggac agaaatgata ttttaacaaa attaaattcg
     7561 tttaccccaa ctcgcacatt attgaaagct gacttttggt cagcttctgt gttggggccc
     7621 cgtc
//
LOCUS       DAHHYJ010000124         7489 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000124 DAHHYJ010000000
VERSION     DAHHYJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.124"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(459..1151)
                     /locus_tag="NBK22_002120"
     CDS             complement(459..1151)
                     /locus_tag="NBK22_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD2415298.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFNFAGVTKEKCIEAPGYRVKESPVHFECEYVQTIRIPTGDPVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPAASKEELAGLE
                     GRNFDNQSDEKI"
     gene            1387..1845
                     /locus_tag="NBK22_002121"
                     /pseudo
     CDS             1387..1845
                     /locus_tag="NBK22_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1832..2134
                     /locus_tag="NBK22_002122"
     CDS             1832..2134
                     /locus_tag="NBK22_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415299.1"
                     /translation="MRTNDHITDLDAIELSSGWNYNSKLETGKNALLSDSLLRDIKVL
                     SSNAKGDADILETELRAVINSMSQNEGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            2288..2647
                     /locus_tag="NBK22_002123"
     CDS             2288..2647
                     /locus_tag="NBK22_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415300.1"
                     /translation="MILRYATNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRLASLSTIGILSVAAYKHFEAGQGKKGAQHALNL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2732..3343)
                     /locus_tag="NBK22_002124"
     CDS             complement(2732..3343)
                     /locus_tag="NBK22_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD2415301.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTELSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGIFTIKRPEK"
     gene            complement(3422..3898)
                     /locus_tag="NBK22_002125"
     CDS             complement(3422..3898)
                     /locus_tag="NBK22_002125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCD2415302.1"
                     /translation="MAVVYVAGGCLWGVEAFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGQKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3901..4401)
                     /locus_tag="NBK22_002126"
     CDS             complement(3901..4401)
                     /locus_tag="NBK22_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2415303.1"
                     /translation="MVLRKVIMQDLDQIIALENIGFSPEEAATREALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFETLAKNHHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4636..5403)
                     /locus_tag="NBK22_002127"
     CDS             complement(4636..5403)
                     /locus_tag="NBK22_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCD2415304.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILQGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5400..6092)
                     /locus_tag="NBK22_002128"
     CDS             complement(5400..6092)
                     /locus_tag="NBK22_002128"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCD2415305.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIQDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(6109..6771)
                     /locus_tag="NBK22_002129"
     CDS             complement(6109..6771)
                     /locus_tag="NBK22_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCD2415306.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2488 a         1322 c         1021 g         2658 t
ORIGIN      
        1 aatactcatt ttgttaaaat ttgtaaaaat tcatttttat tcgtctaaat gtaatcgttt
       61 tcatattttt aaaattactt tttctcgttt atgcgtataa tcttttttta tataaatttg
      121 gctaattggc tttatgttta atcattataa ttgtttcgtt tttaaaataa ttattgtatt
      181 aatatatcta taccatccac cttttattta taaatagtta atttacaact aaacgataaa
      241 tattatatgc aaaatacatc tttaatatta aagtaatacc aatatttttt caataaacct
      301 agtgtaatat atgtgtaatt ctaaaagatt cttctttaaa aatataaata ccacgacata
      361 ttgctttaac attttcattt ataaagcgaa aaaatgcatc gctactaagt tgaatgttta
      421 gtaaggatgc attgaattca ctaaaatgat taaattactt atatcttttc atctgattga
      481 ttatcgaaat ttcttccttc taaacctgct aactcttctt tagaagctgc aggtgctttc
      541 atttcaaata tctcattcac tactgtgtaa tcgtaatatc ctaatctggc aataggttta
      601 atcgacttaa tgtccaattt accattatca agaataacct tatcgtcaat atgaacttgg
      661 gcaactcttc ctataacaat atctacggta gatactggat ctccagttgg aatacgaatc
      721 gtttgaacgt actcacattc aaaatgaact ggcgattctt ttacacgata tcctggagct
      781 tctatacatt tttcctttgt tacacctgca aaattaaatt catcctcttc tggtggcaat
      841 gctttcgatg ataaattaac tgcttctctt aaatcatacg ttgccatatt ccacacaaac
      901 caacctgtct cttcagcatt tttcactgta tctttacgtt cgtgatcacc aagaacggat
      961 tgatttgctg cgaacataac cataggcgga tcccaagtta agttttgata ctgactataa
     1021 ggcgctaaat tatctttccc atctttcgat acagtagaga tccaccctat tggacgtggt
     1081 actgtactac ttttaaatgg gtcgtgcggt aaaccatgac ttcttacacc ttgttttggc
     1141 gaataattca tactatcttc accccttata agtaattaca tttaaggtta cgccctcttt
     1201 acataagcgt ctaatataaa taaacaattt atttataagt agaaactata tatgacgtgg
     1261 ttgcttataa tttgcgttct tgattcgaaa aattcagata aggatttata caattaatat
     1321 ttatgatatc ttttgtaaat ttaattaatt atagttactt caatcatgat tagtttataa
     1381 taataaagtg aaattgaaaa agacagctat tatgcgatga gcgaaaaact tcaagtaaaa
     1441 caagaattta acggcgttac aaggcaatac ttgaatttca gaacagatag tatcgatgat
     1501 ctggataagt tcaaaaaaga attcaaacct gttatgcact taaaaggtga tgcattcaat
     1561 caacagttac aatctttgat taacaaatat ccacaaatac aaaaaaatat gaaatcagag
     1621 ttcattgctt attatgataa agaaaaaaat agagaaacag taaaaaacta tgcttggaac
     1681 cttcaaaaat ctataaatga cattatgcaa tcatatccta gcacaaaatt tgtacagttt
     1741 tataaagatg atgtttcccc gtcaatggta gatggaaatg gccgtttaaa atcggatact
     1801 aatgtaattt ccatcgaggg aggaaagtat gatgagaaca aatgatcata taaccgattt
     1861 agatgctatt gagttaagta gcggttggaa ttacaattcg aaattggaga ctggcaaaaa
     1921 tgcactacta tcagatagcc ttttaagaga tattaaagta ttaagttcta atgctaaagg
     1981 cgacgctgac atactagaaa cagaacttcg tgctgtaata aactcaatgt ctcaaaacga
     2041 aggtaaatct gttgatgcac ttaaaaatag taccgcttct ctaaaaaaca atctaaccaa
     2101 attaaaagag caactcgaca aaactacaca ttaatttcat acattttgag tgaacagaat
     2161 tatatttcca tagcaaacat tcctaaacta cacatacgtt acataattga ttcattttta
     2221 tagaaacggg taaaaatgat aaagtaatca tctttcacaa aaaatcaaat attgggagga
     2281 attaaatatg attttgcgtt acgcaacgaa tttaaaactt gcgaaagagc tataccaagc
     2341 cagtaaacct aaattgcaag aagataaggg tatgattgaa ctgtttgaac atacttttgg
     2401 cttgcaaaaa gaacttgtaa aatacgtcgg cattgcagaa gcaactactg ctgctttata
     2461 ctcagctagc tttatcaata aaaacatttc tcgccttgca tcactatcaa cgataggcat
     2521 tttaagtgta gctgcttaca aacatttcga agctggccaa ggtaaaaaag gtgcacaaca
     2581 tgcattaaat ttattaggtc ttgctacttt aagtttacta gatacatttt cttgtaataa
     2641 aaaatagtct tcattttaga agcacatact ttagcacctg tgtacttttg attcaattca
     2701 taaacggtat accgctatag catgtgtttt attatttttc tggtctcttt atcgtaaata
     2761 ttccccctaa tttagcataa tcatttccgg ctaaacgaga aacagcctgt aatgcgtgtg
     2821 gattgatttt aaaatcaccc tcataaatac tgtcatcaat atgataagtt acaatttcac
     2881 ctattattaa atcagcccca tctaatacat ctccaagcaa tatcatttgc gatagtttac
     2941 attcgaatct cattttcgca tctttaattc ctggcgtctt aatcgttgta gatgttaaaa
     3001 gtgataattc tgtacgactc aactcactgt caccatatgc taacggcgct gcagtctcat
     3061 taatatcttg aacattatct tcgtctgtaa tatgcacaac aaagtctcca gtccgttcta
     3121 tatttaatgc agtatctttt ctcttacctc ctgcacgttg aactgcaata gcaatcattg
     3181 gcggatgatt attaacaata ttaaaaaagc taaatggtgc tgcatttact gatgcatctt
     3241 gatttaatgt tgtaacaaaa gctataggtc gtggaataat tgaaccaatt aataatttat
     3301 agttttctct agcagttaat gattgtgcat caaacgtata cataatacct acctcttttc
     3361 taagtatatc taggtatttc tccgattttg gttaatttaa acatctattc tcctctgaaa
     3421 atcacttgta tttatttagc aaatcttttg aaatatgaca catatgcata tcttctggat
     3481 atttttctaa atgttgctga tgttcttcag cacttttaat gtagttagac agcggtaaga
     3541 cttccactgc aatttgatct ctgtctttac gtcgttcaat gaactgacgc gcttcaatta
     3601 agtggtcatc tacacaacta tataaacccg ttcgatactt ttgtccaata tcatttcctt
     3661 gttgattcac actgtaagga tcaatgattt caaataaata attcataatg tctgtaattg
     3721 ttaacatacg atcatcgaaa tgaagtttga cacattcagc ataaccatca tacggaccgt
     3781 ctaatttaga gcttcttcca tttgctcttc ctgcttctgt atgtataatt ccaggtattg
     3841 ttgcaaaaaa tgcttcaaca ccccataaac atcctcctgc tacataaaca actgccatat
     3901 ttacacctca tcatcctttt ttatattttt aacaaggtta taccatttaa taccgccatg
     3961 acatgattct gatacacctt cattacgata cccatatttt tcataaaatg aaattaatga
     4021 ttctcgacat gttaacgtta caccatgtcg atgatgattc ttagcaagag tttcaaaata
     4081 gtttagtaag cgacctgcaa taccctgacc ttgataattt ggtgctacaa caagacctaa
     4141 cacactaata tagccacctt cactattatt tgtggagaca tttttaaata aatcatcgct
     4201 aatgtaacgc tcttttatga ctggaccgtt gatgtatcca attacttcat cattcttttc
     4261 agctacaata aaagtttctt gaatttgttc aattctcaat ttcaatgcct ctcgtgttgc
     4321 agcttcttcc ggtgaaaaac caatattttc aagtgcaata atttgatcta agtcctgcat
     4381 gattactttt cttaatacca taaaaacact ttcttagccc tatatatcaa aagtaatacc
     4441 ttcatcttta tattcattat tttcaaacta ccttcactat aaaaaacgag tctttttatt
     4501 gaaagccatt cgccttaatc aattagacaa gttgtattat acgaattagt aaataatcat
     4561 gattataatt cattttaagc atactcaaaa agactggtac atgtatacca gtctaattcg
     4621 aaaaaatata tttagttaaa accatttcaa aaacgacttc acttttcctg gcttagtcgg
     4681 tctgtctaaa taaacaaaat tatcattgat tactgaactg gcatttcgaa gtaatgcttc
     4741 aatatcattc gaatatttct tcaatttatg attgtgaaat aattcttgca tcaaaaatgg
     4801 tctttggtca catgaatgtg catctgaagc tacaaaatga gccaaattac attctataaa
     4861 ttgtaatgat aacttttgaa tgttttttcc aaatccacca actaaagaac tcgatgttaa
     4921 ttgactcaat gccccatttg caacaagttc atataatatt tctggatttt tggcgatact
     4981 tctatttctt tcaggatgtg caatgattgg tatgtagcct ctcgattgta tttcaaaaaa
     5041 caattgtttt gtataatgtg gtacttcgcc cgttggaaat tcaattagta aatatttcga
     5101 acgattaata ccttgaatac taccattatc taagccttgc agaatcgaat ctgtaattct
     5161 aatttcttgc ccgggaaata atttaatatc caatgcttga acttctggat gcgttcttaa
     5221 ctccgccaat ttcacaagca cttgttgaaa tgtattatca tatctcggat gcaaatgatg
     5281 aggtgtcgct acaatacttg ttacaccttc atccttagct tgctttaata gtgcaatact
     5341 cttttcaatt gttttaggac catcatctat atcaactaat atatggttat gaatatcaat
     5401 catgattcat cagtcccata atatgcatag taactagcac ttttatcttt aggcattcta
     5461 tttaagacta cacctaataa tttagcacct gttgcttcga taagttcttt tcctttttta
     5521 acttcatctt tattattatt ttccgaatta actacgtaga caacattgcc ggtaaacttt
     5581 gaaaataatt gcgcatctgt aactgtgttc actggtggcg tatcgataat gacaaagtta
     5641 taattcatca ataatgtgtc atacaaattt gcaaatgccc ttgatgtaat taactctgac
     5701 ggattcggtg ggattggccc agacgtcaag acgtctaaat cttgaatttc agttgagata
     5761 atactgtctt gataagttga ccaatttagc aataaacttg aaagtccttc attgtttggc
     5821 aaattaaaaa tataatgctg cgtaggttta cgcatatccc cgtctacgat tagtgtttta
     5881 taacctgctt gtgcatatgc aactgctaaa tttgctgcaa ttgtagactt acctgcgcct
     5941 ggtgcctctg aagtgattac aatgctttga actgcactgt caggatttgc aaacataata
     6001 tttgatctta tgcctcgaaa tttctcgcta ataggtgact ttggttgttc atggacaatt
     6061 aaacttgatg tacttcttcg tgtattcgtc atggtaattc ctcgtaaatt aaaatttttg
     6121 tattgaacct aaaataggta atcctagttg cgattcaaca tcttcttccg tcttaatacg
     6181 cttatctaat aattctttta agaaaataat caatattgct aaaacaatac caacaataat
     6241 gctgatcact aagttgacag atactattgg agatactttt acagcattat catgtgctga
     6301 ggaaagtatc gtaacattat caacactcat aattttaggc atgtcatgag caaaaacttt
     6361 agatatttta ttaacaattt tgtcagattc agatttattc ccagtggtaa ctgatacagt
     6421 aataatttga gagtttgttt gattggttac ttttaaaaat gaattcaact cagctgttga
     6481 atactgacca tcaaattctc tagatacttt atctagaatt ctaggacttt tgataatttc
     6541 cgtatatgta ttaacagact gcaaactact ttgaacattt tgaaaagcta aatcacttga
     6601 agactttttc atgttcacta atatttgagt agaagcagta tatttgtcag gcataacaaa
     6661 aaaggttaat gccgcactta ctacaagaca tattgccggt aaaataagca ataatttaat
     6721 attcttcttt agaatattta atagttttac taaatcaaac ttttctttca tggtttcctc
     6781 cacataatca atcattgtat tcattatgta tgttttataa atcggacaat tatatctagt
     6841 ttaacgacta caaaacatac acaactacat tttctctaat tatttatata aatattttat
     6901 cgtttaaaat tatatcacga ttcttgacca ttatgtataa cttatttata tttttgtaca
     6961 agatataata ttgtccaact tcaaatatcc aaacctatta ataataaacc tagataccat
     7021 cgtacgctat catggctttc ttataatcga gtagaagcat catcattact tgattatttg
     7081 ctctttacaa caccgagcgt gcccgtactc ggtagttcaa taccttgcgt aacccgtcac
     7141 tgtgaattgg gttaatgata ataaagacca caccttttaa aaagatgtgg gtaatttata
     7201 taatttttat ttacattttt aacttataaa aaaaagcgcc tatgtcatga tttaccatca
     7261 cataggcgct tatcaataaa ttattactta ttactttcca tttcatctaa tttatgcgga
     7321 tttcctgtaa ttagatgaca acttattctt ttcaggggaa cattacactt ttataatatg
     7381 ttcaaagaca aacttaacca ttcacaaata taaagaataa tattatcaaa tcattgaaca
     7441 aatcgtattt tgcaacaatt gatatttata ttaatgtatt gcatttaat
//
LOCUS       DAHHYJ010000125         7193 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000125 DAHHYJ010000000
VERSION     DAHHYJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.125"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(170..1435)
                     /gene="lysA"
                     /locus_tag="NBK22_002130"
     CDS             complement(170..1435)
                     /gene="lysA"
                     /locus_tag="NBK22_002130"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCD2415307.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPSRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPEINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEEADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDTK"
     gene            complement(1425..2510)
                     /locus_tag="NBK22_002131"
     CDS             complement(1425..2510)
                     /locus_tag="NBK22_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD2415308.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYN
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSITLKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            complement(2515..3666)
                     /locus_tag="NBK22_002132"
     CDS             complement(2515..3666)
                     /locus_tag="NBK22_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD2415309.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDAGQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            complement(3809..4528)
                     /gene="dapD"
                     /locus_tag="NBK22_002133"
     CDS             complement(3809..4528)
                     /gene="dapD"
                     /locus_tag="NBK22_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCD2415310.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            complement(4555..5277)
                     /locus_tag="NBK22_002134"
     CDS             complement(4555..5277)
                     /locus_tag="NBK22_002134"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCD2415311.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVHALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGFYTFDNL"
     gene            complement(5274..6161)
                     /locus_tag="NBK22_002135"
     CDS             complement(5274..6161)
                     /locus_tag="NBK22_002135"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCD2415312.1"
                     /translation="MTHLFEGVGVALTTPFTNNKINIEALKTHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKTVIDLVDKRVPVIAGTGTNDTEKSIQASIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVEILSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNSFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            complement(6163..7152)
                     /locus_tag="NBK22_002136"
     CDS             complement(6163..7152)
                     /locus_tag="NBK22_002136"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCD2415313.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQGKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
BASE COUNT         2163 a         1385 c         1109 g         2536 t
ORIGIN      
        1 ataaaggatt aatgacaaaa agaaataaat actttctaac taaaaatgtg ctgttcttcc
       61 ttttttagag tatatgtcca agcatgtttt ctacagtcat ctataatgca taaaaattcc
      121 ccgagtcaca gtataatttt ctgtgcttcg gggattactt tgtttatttt tattttgtat
      181 cattaataat gagttgtctt aacgattgtc gctttataac ttcacgtgct ttgccatctt
      241 ttaagaaaaa cacagaaggc ttttgcattt gattgtaatt ggatgccata gagtaatgat
      301 atgcaccagt tgataatata gcaagatagt ctccacgttt gactgatgaa ggtaatttag
      361 cgtctttaat aatgatatca ccagactcac ataattttcc agctatagtt acactgtcat
      421 ctgcttcttc atttctatta acaagcaatg cttgatactt tgcgtcataa agtgcagttc
      481 tgatatgatc actcataccg ccatcgattg aaacatattt attaatctct ggaatttctt
      541 taatggttcc aacttcatat aaagtaacgc cagcttcacc tacaattgat cgtcccggtt
      601 caatacctat ttctggtgca tctataccta gaactttaat ttcggatttt attgcgtctg
      661 taatatcttt aataccactt tcgataggga aactttcgtc accttcaaca tatttaatac
      721 caaagccacc accaaggttt aataattcaa cttgaatgcc ttgctcttta agccaacgta
      781 aaacaatttt agcagtttca ataaatgctt ctgtaccttc aatctgtgaa ccaatatgac
      841 aatgtacacc ttttaatttt aagtgtttag attgttggac tttgtcaatt gcttttttag
      901 ctaagccata ttgaattgat aatccaaact tactatcttc ttgcccagtt tgaataaatt
      961 cgtgtgtatg tgcttcaaca cctggattaa ctcgtaatac aacttgaacc gtatcattag
     1021 catagcggtc tattaattca atttcttcta atgaatctat aacaaaataa ccgatattat
     1081 tttctaaagc atacctaatt tcatgtttcg ttttattgtt accatggaaa tggatgcgac
     1141 tcggttcaaa acctgcttct aaagctgtat ataattcacc ttcagaaaca acatctaact
     1201 gtaaatcttc ctcagctaca agtttgacca tttgaatgca agtaaatgcc tttgaggcgt
     1261 atgaaatatt gtattttaat ccactatctt taaatgcgcg atggtaacgg cgcatctgtt
     1321 ctctaatttg tagttcatca taaacaatgg taggtgtacc aaagctttgt gcaatcgttt
     1381 ttaaactaat accatccatt gttaattcgc cattttgatt atatttaaca gtcatttgaa
     1441 atgtactcct tttttaaaga atcatgattc atagcactta attgttcaga atttgcacca
     1501 acacctttaa aggtatattc atcgatgcgg atatcattat tataaagaat aacttcatct
     1561 tgtgcatgta cattgccatc tacttcaaca aacatatggc tcatcattaa tgcacgtatc
     1621 gggtagcgtt taccattgat aagtgcttca tgtttagcac gagttcttaa aattccatcg
     1681 ccataaccga tatcaactac agctaatttt gtattgtttt tagtcacttc aaaggcaaag
     1741 ctataaccgc aataatcacc agcttgtact tcgcgcactt gaataacatg tgcttttaaa
     1801 gttattgact gaactatatc atgttgattc agtgaactat atggtcttga accgtataac
     1861 gcaataccta cacgcgcatg tgtatggtgg ggtagtaata tttgtccttc ccgataaaaa
     1921 ctcgcactat tttgagcatg gattaggtcg aactgataac cttcagataa aagtgcttca
     1981 acaatttcca tccattgtga acgttcaaca ttataatctg acacatcgaa ttcatcagca
     2041 tatccaaaat gggtccataa accactaata atcatttttg cattttgatt atgatggtga
     2101 tctttcaata cttctttaat ttcgtttaaa tctttaaatc cagaccgatg taataaattt
     2161 tcaaattcta agtgaacatg aataccagct aaatcatttt tatggttata gtaatatgtc
     2221 aacgacggca aagtcatgtg tatttgatgt tcacggacta aatcaaactc gtaaactgca
     2281 ttcattaaaa agattgttgc atctggagca agttgtctaa tttgaattgc ttctcgtagt
     2341 gatgttgtgc taaatgtatc tatacctgca tggataaact gagttacagc aaattctagg
     2401 tcatagtgat atgcattatt tttaacaact gccattaatg gctgattgtt tttgactgtg
     2461 attgcatttt gtaaaaatat tttcttattt acagaccatg ttgctgtcaa tgtattacac
     2521 ctctttgtaa ttatttaata aattttcgta aaaattaacc acgtttatta acactttttc
     2581 atcaaaattt aaatgtgatg tgtgcaaacc agttacaaaa cctttatctt catttcgtgt
     2641 tcctataaaa acaaagtaag ctggagctag ttgttgacca taaaaactaa aatcttcccc
     2701 aaataagaat ggcgttggtt tgtcatagac atttaaatca gcttttatta aggcgtcctc
     2761 tatttgagta cgtaatttcg gactattgat tgtaggggga taaccttctg caaatttaac
     2821 ttcacaatct acattaaaca gaagcttgac actttctgct atcttgtgca tttgattttt
     2881 aacgattgtt aaatcatcaa tatcatatgt acgaatagta ccttctaaat agccattact
     2941 tggtacagtg ttaatcgctt caccagcttt aaaatgacca atatgaacaa tatttcgttt
     3001 caaaccgtta aggtgaaatt gttgaatttg tgatatttga gttaatacat gttgtaatgc
     3061 ttcaccacaa gaatgacctt gttctttatc agcaacatga cttgacaggc ctgttaagaa
     3121 aaagcgatac tctgttgcgc tggccgtaat ttcttcatct cttatcactg caatgccttc
     3181 atcagcaaat gggttaacat gaataccaaa taccgcttca attggatact tatcaaaggc
     3241 accggctttt attaatcgat ttgcaccgcc accagtttct tctgcaggtt ggaaaatgaa
     3301 aacgacattt tgcggtaatt gacctgcatc ttgcatgtct ttgcaacgtt gtacaaaaag
     3361 cattaatgca gttgtatgac catcatgtcc acaagcatgc atcacatgat cagattgact
     3421 gcgataaggc acatcatttt cctctaaaat aggtaacgca tcaatatcag ctctatacgc
     3481 tatcgtatgt gagccattac cttctaagta tgcaatgacg ccagtttcca atgggcaatc
     3541 gtatttaata tttaaactat ctaaaaacgc tttaatataa gcagttgttt caaattcatg
     3601 taagcttaat tcaggatgtt gatgtaaatg acggcgatgt ttcgtaacaa attctaattc
     3661 attcataatt atcaatcctt tgtgttaaat tactatataa atagtgtaac gtatttcgaa
     3721 atttgtgatc ataagtttat tcaatgataa acaataaggt tgagacataa tcgtatctca
     3781 accttgaaat tattatacgt tgacgtcact agtcattcag ttttcttaat gctgctacaa
     3841 tctctttttt agtatcttgt acttcagaag cttgcttaat cacttttgca ggtgtaccag
     3901 caacaactgc accagctggt acatcttgtg tcacaatcgc gccagctgca acaatagcac
     3961 ctttaccaac acgtacacct tctaaaataa ctgcatttgc accgattaat acatcatcct
     4021 cgattataac cggtgatgca ctagggggtt caatcacacc tgctaatact gcgccagccc
     4081 ctacatgtac atttttacca gttgtagcac gaccaccgag agtagcattc atatcaatca
     4141 ttgtaccttc gccaacgact gcgccaatat taattgttgc gcccatcata acgacagcac
     4201 catcttcaat aatggcttgt tctctaataa acgcacctgg ttcaattcgt gcattcgtat
     4261 ttgttaagtc ttttaatgga atggcagaat tgcgacgatc catttcaatt tctatatctt
     4321 cgaattgact accatatgct tcgtaaaaag gtttccaatc atccgcttca caaaagatta
     4381 ctttagattg ttctgaacca aatactttaa aactttctgg atatgtgatg ccttcaaaat
     4441 taccatttaa atatactttt attggtgtag actttttagc atcacttata tattgaataa
     4501 tttcttcagc tgttaaatgt tgtaccataa aataatcgat ctcctttaat atgtttataa
     4561 gttgtcaaac gtataaaagc cgtttggttt attaactaag cgttctgctg cttgtattgc
     4621 accattcgca aaaatatctt ttgattgtgc acgatgcgtg atttgaatcg tttcatcagt
     4681 gccagcaaat agaacttcat gttcaccgac aatcgtacct ccacgaatag aatgtatacc
     4741 aatatcttgt ggctggcgtt tttcatttaa ttcatgtcta tcatacacag gtgttacatt
     4801 ttctttcaaa gatacgatca catcatacaa tttttctaac gtaccacttg gtgcatctac
     4861 ttttttatta tgatgtgcct cagtcaattc gatgtcgaaa tcatcaagta ggggaacagc
     4921 agctgctaaa attttagtca atgcatgaac gccataactc atgttcgcgc tgaaaaatac
     4981 aggcatattt tgactcaatt catctaactt attaagtagt ttttctttct cgccagttgt
     5041 tgccacaact aatggcaaat gaaactcttc atctaataaa gggaacagca gatttggatt
     5101 tgaaaaatct attgcaacat cggcatcttt aacatctgca atatgttgat attgttgata
     5161 tggcgttgtt gctttcggtg tattttcaat gaccccaacg atttcatgtc ctttttcttc
     5221 tgctaatcta gcaacgcgtt gattcattgc accatagcca attagtaata ttttcactca
     5281 ttttcacccg ctttaaatgt gtcatatgct tcacgaagca ctttagtatc tgtatcttct
     5341 aggctaacca atggtaaacg taattcataa tttccaaatc ctaaataact tgttaaagct
     5401 ttaataggaa ttgggttaat atcaactgat agggctgata acagtgtgcc gattggttta
     5461 aattgatctt gaatatctaa tccactttgt tgagcatcgt ataacgcttg aaattcttta
     5521 ggaatgacat tggcaataac agagataacc ccttgaccgc cacgttgata gtattcaacg
     5581 acgttgtcat cattgccact atataatgca aatgaatttg tatcaattcg ctttttcact
     5641 tcttctaaat actcaaaatc attcgtagca tcttttaaag caactatata aggatgttga
     5701 cttaatattt ctacagtttc tggttcaatt gtcatgttcg ttcttgaagg aacattgtac
     5761 agcacgactg gtaatttcac agcatctgca atcgcttcaa agtgtttgac taaaccacgt
     5821 tgattcgttt tgttgtagta gggcgtaatt aacataattg catcagcccc taaggcttta
     5881 gcttggattg aagcttggat tgacttttca gtatcattag tgccagttcc tgctatgaca
     5941 ggaacacgtt tatctacaag atcaataact gtttttagaa tgcgttcttt ttcatctgtt
     6001 gttaaagtag ggctctcagc agtagttcca ttaacgatga ttgcttgggc attattttct
     6061 agtaaaaaat taacgtgtgt tttcaaagct tcaatattaa ttttgttatt tgtaaaaggg
     6121 gttgtaagtg caacgccaac accctcaaat aaatgtgtca ttttaattcg ctccttttaa
     6181 acgcataact tgttccaata cttgtacagc atttaatgca gcacctttta ataaattgtc
     6241 tgatgtacac catacatgga aagtattttc taatgaatca tctctacgta tacggccaac
     6301 aaacacttca tctttattag tagaattgat tgccattgga tattcattgt tctctggatt
     6361 gtctactaaa acaacgcggt catcttgatc aaataacgct ttaatatctt ctgctgttgt
     6421 ttctttgtca agcgttacat caatttcaac actatgacta tcttgaacag gcacacgtgc
     6481 gcatgttgct gttactttta agtctggcgc atttaaaatt tttctcgtct catcaatcat
     6541 tttttgttct tcttttgtat atccgttttc taaaaacaca tcaatatgcg gtaacacatt
     6601 attataaatt ggatgtggat atgcttctgg tgctttacca tttacacctt cagctaaatc
     6661 tttcttacct ttcatccctg aacctgatac agcttgatat gttgtatatg ccactcgttt
     6721 taaaccataa gcatcttgca atacttttag aggtacaaca gattgaatcg tagagcagtt
     6781 tggattggca atgatacctc ttgtaaatgt aggttcattg acttccggaa cgattaaatc
     6841 aatatcttct gccatacgcc attgacttga attgtctata acgattgcac cagctttttc
     6901 aaaaagtggg gcaaagtgtt cgcttgtacc gccaccagca ctcattaata cataatcgaa
     6961 atgttcactt gcacgagcat cagttaattc ttgaactgta tatgtttttc cttgaaattc
     7021 aacttcttgc cctgcagaac gtgctgatga aaataatact aattcatcga aaggaatatt
     7081 tttacgattt aatgtctcca acatttttgt acctactaat cctgttgcac ccacaactgc
     7141 taactttgtc ataacttgtc actccatttt ataataattt ccaattttta gaa
//
LOCUS       DAHHYJ010000126         7056 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000126 DAHHYJ010000000
VERSION     DAHHYJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.126"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(11..1372)
                     /locus_tag="NBK22_002137"
     CDS             complement(11..1372)
                     /locus_tag="NBK22_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD2415314.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAFKTQFGHASFTNLYEHGIFAKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKDPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKNA
                     TYKLLGGKYMGYLIFVFFIFVFGLLFINVDTRRAIYFIPIWFILLAFMYLRYKRIAAK
                     SNK"
     gene            1720..2841
                     /locus_tag="NBK22_002138"
     CDS             1720..2841
                     /locus_tag="NBK22_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCD2415315.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            2890..4158
                     /gene="icd"
                     /locus_tag="NBK22_002139"
     CDS             2890..4158
                     /gene="icd"
                     /locus_tag="NBK22_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCD2415316.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVEKEGRDAANAAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            4548..5252
                     /locus_tag="NBK22_002140"
     CDS             4548..5252
                     /locus_tag="NBK22_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2415317.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVES
                     EKPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            5252..6913
                     /locus_tag="NBK22_002141"
     CDS             5252..6913
                     /locus_tag="NBK22_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCD2415318.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLLCLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVVT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
BASE COUNT         2544 a         1011 c         1319 g         2182 t
ORIGIN      
        1 aaacttgttg ttatttattt gatttagcag cgatacgttt atatcttaag tacataaatg
       61 ctaaaagtat aaaccaaatc ggaataaaat aaattgcacg tcttgtatca acattaataa
      121 ataataaccc gaacacaaaa atgaagaata caaatattaa gtagcccata tatttaccac
      181 ctaatagttt gtacgtagca tttttatgta gatctgggtt tttacgacta taattgatat
      241 atgcaatgat aatcagaccc catacaacta aaaataacac tgtagagatg gtagtcacat
      301 acgtaaatac ttttgtcgca tctgggaaaa tatagtttag taatgctgcc acaagtaata
      361 atgctgatga agcaaagatt gcaacatgtg gaacgccata tttattcgtc ttagaaaagt
      421 tcggaggtgc ttgttgttga cttgataaac cgaaaagcat acggctattt gagaatatac
      481 cactattaca tgatgaagca gcagcggtta atactacaaa attaatcaag cccgcagcaa
      541 acggaatacc gatcaatgcg aataatttta cgaatggact gttatcagga tcaacttgct
      601 gccaagggat aatagacatg ataaccgcta acgccccaac gtaaaatatt aaaattctaa
      661 tgggtacact gttaattgct tttggaattg ttttaactgg atcttttgtt tccccagctg
      721 taacaccaat catttcaatt cctacaaatg agaatagtgc catttggaaa gacataaaga
      781 atccagaagc acctttagcg aatatgccgt gttcatataa atttgtgaaa ctggcatgcc
      841 cgaattgagt tttaaatgca aatagaatca tgacgaaacc aactactatt aaaccaataa
      901 ttgtcgctat tttaatgata gagaaccaaa attctaattc tccgaaaagt cttgcgctaa
      961 gtaggttgaa tgacattaat aacagtacac aaaatagtgc acttatccag tttggaattt
     1021 ctgggaacca aaagctaaca tattttgcca cagccgttac ttcagccata cctgtaataa
     1081 tccaacagaa ccagtatgtc catccggtaa caaatcctgc aaaaggccca atatatgtat
     1141 tggttacatc tgcgaaagat ttaaattcag tattctgtat aatgatttct cctaaacctc
     1201 gcataaacat aaataacata aatcctataa tgatgtatgt taatagaatt gaagggccgg
     1261 ttaatgcaat cgtttgacca gcacctaaga ataagcctgt accaattgca ccgccaattg
     1321 caattaattg tatgtggcga ttgctcagtt ccctttgtaa tttttcagcc ataatacatc
     1381 tcccttaaat atagatatgt ttattatgca cttatattga gatatataca attattttcg
     1441 gtaaaaatgt gtaaaattcc atgttaatat actttggttt ttataatcat atataataac
     1501 caattgaaaa tttaattcta ttgtaaaatt catggattat tcacatcttg aaaaagcttt
     1561 aatggtgcta tttgtggcta ttctgtgaca tttacataga tttacaaaaa aattgttgca
     1621 catataatgc cagtttttat atttcacaaa cgaaatgcgt ttagctataa tattagttga
     1681 aagccatttc ataaagaaac agtaaagggg aaatttatca tggcagaatt acaaagaggt
     1741 ttagaagggg ttatcgcagc ggagactaaa ataagttcaa ttattgaaag tcaattgact
     1801 tatgccggct atgatattga tgatctagct gaaaatgcgc aatttgaaga agttattttc
     1861 ctattatgga actatagatt gccaaacgaa gaagagcttg ctcatctaaa agggaaatta
     1921 aatcaataca tgacattaaa tcctcgtgtg tatacacatt ttgaggagta tgttacagat
     1981 cacgtgcatc caatgacagc attacgtacg tcattatcat atattgcaca tttcgatcct
     2041 gatgctgaaa atgaatcaga tgaaaatcgt tatgaaagag caatgcgtat acaggctaaa
     2101 gtagcatcat tagttacagc gtttgctcga gtaagacaag ataaagaacc acttaagcct
     2161 aatcctgact taagttatgc ggcaaacttc ctatatatgt tacgtgggga attaccaaca
     2221 gatatagaag tagaagcctt caataaagca cttattttac acgctgatca tgagttgaac
     2281 gcatctgcat ttacagcacg ttgtgcggta tcatcattgt cagatatgta ctcaggtatt
     2341 gtagcagctg taggttctct gaaagggcca ttacatggtg gtgcaaacga acaagttatg
     2401 acgatgttat ctgagattgg gtcaattgaa aatgttgatg cttacttaga tgaaaaattt
     2461 gctaataaag ataaagtaat gggcttcggt catcgtgtat ataaagatgg tgatcctaga
     2521 gcgaaatatt taagagaaat gagccgtcaa attacgaaag acgctggtcg tgaagaatta
     2581 tttgaaatgt cagtgaaaat ggaaaaacgt atggcagaag aaaaaggatt aattcctaat
     2641 gttgattttt atagtgcgag tgtttatcac tgtatggaaa tacctcatga cttattcacg
     2701 ccaatctttg ctgtaagtcg ttctgcagga tggattgctc atattttaga acaatataaa
     2761 gataatagaa ttatgcgtcc tagagcgaaa tatattggcg aaacgaatcg taagtatatc
     2821 ccgcttgaag aaagaaaata atcaatacaa attaaaaatg aagatgtaaa atttggaggt
     2881 aaaataacta tgactgcaga aaaaattact caaggaactg aaggattaaa cgtacctaat
     2941 gaaccaatta tcccatttat tatcggtgat ggaattggac cggatatttg gaaggcagca
     3001 agccgagtta tagatgctgc tgttgagaaa gcctataatg gcgaaaaacg cattgaatgg
     3061 aaagaagtgc tagctggcca aaaagcattt gatacaactg gtgaatggtt acctcaagaa
     3121 acacttgata caattaaaga atatttaatt gctgttaaag gacctttaac aacaccaatt
     3181 ggtggtggta ttagatcatt aaatgtggct ttacgccaag aattagattt atttacttgt
     3241 ttaagaccgg tacgttggtt taaaggagta ccatcacctg ttaaacgtcc acaagatgtt
     3301 gatatggtta ttttccgtga aaatactgaa gacatttatg ctggtattga atttaaagaa
     3361 ggtacaacag aagttaaaaa ggtaattgac ttcttacaaa acgaaatggg tgcgacaaac
     3421 attcgattcc cagaaacttc aggtattggt attaaaccag tttctaaaga aggaactgag
     3481 cgattagtta gagcagctat acaatatgct atcgataata accgtaaatc agttacttta
     3541 gttcataaag gtaatattat gaaatttaca gaaggctcat ttaagcagtg gggttacgat
     3601 ttagcattat ctgaatttgg tgatcaagta ttcacttggc aacaatatga cgaaattgtt
     3661 gaaaaagaag gcagagatgc tgctaatgct gctcaagaaa aagctgaaaa agaaggcaag
     3721 attatcatta aagattctat tgctgacatt ttcttacaac aaattttaac tcgtccagct
     3781 gagcatgatg ttgtagcaac tatgaacttg aatggtgact atatttcaga tgctttagct
     3841 gcacaagttg gtggtattgg tattgcgcca ggtgcaaaca ttaattatga aacaggtcat
     3901 gctatttttg aagcaacaca tggtacagct ccaaaatatg caggtttaaa taaagtgaat
     3961 ccatcttcag taattttaag ttctgtatta atgttagaac atttaggatg gcaagaagcg
     4021 gcagataaga ttacagattc aattgaagat acaattgctt caaaagttgt tacttatgac
     4081 tttgcccgtt taatggatgg tgctgaagaa gtttctacat cagcatttgc agatgaattg
     4141 attaaaaatt taaaataagc aaaataaaaa taggtgagtc tagcaacttt aattgctagg
     4201 ttcgcctttt taaatgaatt gaagttggca acaggataga aataataaag agtcactaat
     4261 gattgattat atagtgtttc ttacaaatta gacaaagcga ttgtgtacat aagcttagga
     4321 ataaatgagt aaaactcatg cataggtagt ggcatagaaa tgatattttc aaaaagttta
     4381 tttcgttgtc ccagagtaag tcatgcaaat gatagaagga aatataagaa gtattttatt
     4441 tatatgtaaa gtaatgtaaa aaatgtgtaa agataatgtg taggatgtta tttttataaa
     4501 tagttcggtt ataatgtaaa tataattata agttagggag gcataccatg tcgcaaaaag
     4561 tgttggtagt agatgacgaa cattcaatcg taacattact taaatataac ttagaaacag
     4621 ctggttatga agttgttgtc gcatttgatg gtgatgaggc tttagaaaag gtagaaagtg
     4681 aaaagccaga tttaattatt ttagatgtta tgctacctaa aaaagatggc attgacgtat
     4741 gtaagactgt aagaacaaat aaaaatttag tccctatatt aatgttaacg gcgaaaaatg
     4801 atgaatttga tcgggtatta ggtttagaat taggtgccga tgactatatg actaagcctt
     4861 tttcacctag agaagttgtt gcccgtgtga aagccatttt aagacgttct caatttgtaa
     4921 aagaagtaga agagattgac gatgatatta ccatcggatc gatacgtatt agacccgatt
     4981 attttgaagt atataagcat aatgagttat tggagctaac accgaaagaa tttgaactct
     5041 tgctttattt gatagaaaga caaggcagag tcattacgag agagcatatg ttaaatacgg
     5101 tatggaatta tgaatttgca ggagactcaa gaattgttga tgtacatatc agtcatttaa
     5161 gagataaatt agaagataat cctaagaagc cacaacttat taaaactgtg cgtggcttag
     5221 gctataaatt ggaaagacct aaagaacaat gatgaagttt caccaccgct taatgttatt
     5281 gataagcagt atattaatta tcagttttgt tacattaggc attattattc acgcttccat
     5341 cacatatgtt ttatcagaaa aacacgaaca agctctagca acagatgcaa gatcatatat
     5401 ttatttagtc caagataatg agattgataa agttaaagaa attgtaaaac agcaaaatat
     5461 agatttattg ataactaagc atgataaaaa ggtgtttagt agtggcaaac tgaaagctat
     5521 cccaaatagt gatagctata ttcaaaaaag ggacgttttt atttttaata agaaggttaa
     5581 cggttatcat gtatggatta aaggttacaa taacgatatt acagaaatgc attggacgtt
     5641 gtggaaatat cttattttga catgtttagt tgtactcctt tgtctgtatt ttgcaagccg
     5701 tagttttaag cgtacattaa tccgtcccat tcaagaagtt acttatgcaa cacaattgtt
     5761 ggcaaatggt tattatcaca tacgtgtacc tgaaagtaat gttgtagaaa ctaaagcgct
     5821 ttttgtgtct accaatgatt tagcacgacg ccttcaaaag cttaataatg agcaaaaaat
     5881 tcaatccaac cgtttaaaaa caacgattga aaatattcca agtgctattt taatgataga
     5941 tcgaaatgga aaaatcgttg ttgcaaataa agcgtattat gaacagttca acatctcgca
     6001 taatattgaa caagtaggct atcacggcta tgttaatact gaaattgagc aattaatttt
     6061 agaaagtttt aaagttgaaa aacctattta tgaacaattg gaagttgcaa taaaccaagt
     6121 ccatgccaag tactttgata tttcatgcgt tccgatttta actaggtctc aaaaaagttt
     6181 gcaagggatt ttagttgtga tgcatgacat aacaaatctg aaacaacttg aaaatctaag
     6241 acgtgagttt gtagcaaatg tttcacatga attaaaaaca cctattactt caattaaggg
     6301 atttgctgaa acgcttattg atggtgcaaa aaatgatgcg gaatcattag acatgttttt
     6361 aaacataatt ttaaaagaat caaatcgaat agaatcttta gtaacagatc tgttagattt
     6421 atcgcatatt gagcaacata cagagttaga tacagactat atgaatttat cagatttaac
     6481 gcggcgtatt attgataata tgatgactca agccaatcaa aaaaatattt ccattcatac
     6541 tgatattgaa aaagatgtca ttgttaaagc acaagaaagt aaaattgctc aagttgtcac
     6601 gaatttgtta acgaatgcaa ttaactattc ttatgaagat ggagatatta acgttcgtgt
     6661 gtatcgagat gactttcgtg tcattttcga agtacaagat tttggtatag gtattaaatt
     6721 agaagaccaa caacgtattt ttgaaagatt ttatagagta gataaagcac gaagtagaga
     6781 ttctggtggg acaggtcttg gattatcaat tacaaagcat atagtagagg cccaccaagg
     6841 caatattgaa gtgaatagtc aagttggcaa aggctcaacg ttcaaagtta ttctaaaaga
     6901 ttataaagaa taaaaattaa agtggtaaca gcgcgtgtat ttttcacgaa gttgttgctg
     6961 cttttttatt tcgttcaaat ctctattggt attaaattag agttaatgaa gtggaataaa
     7021 ttcggttgtc aattttgtca tttgtatatg tcgatt
//
LOCUS       DAHHYJ010000127         7050 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000127 DAHHYJ010000000
VERSION     DAHHYJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.127"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            20..988
                     /locus_tag="NBK22_002142"
     CDS             20..988
                     /locus_tag="NBK22_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD2415319.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(1349..1843)
                     /locus_tag="NBK22_002143"
     CDS             complement(1349..1843)
                     /locus_tag="NBK22_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCD2415320.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            2076..3455
                     /gene="uhpT"
                     /locus_tag="NBK22_002144"
     CDS             2076..3455
                     /gene="uhpT"
                     /locus_tag="NBK22_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCD2415321.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCLFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(3814..4572)
                     /locus_tag="NBK22_002145"
     CDS             complement(3814..4572)
                     /locus_tag="NBK22_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD2415322.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLAPCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(4565..6121)
                     /locus_tag="NBK22_002146"
     CDS             complement(4565..6121)
                     /locus_tag="NBK22_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD2415323.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVIIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLSEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(6118..>7050)
                     /locus_tag="NBK22_002147"
     CDS             complement(6118..>7050)
                     /locus_tag="NBK22_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD2415324.1"
                     /translation="IFLSACANTRHSESDKNVLTVYSPYQSNLIRPILNEFEKQEHVK
                     IEIKHGSTQVLLSNLHNEDFSERGDVFMGGVLSETIDHPEDFVPYQDTSVTQQLEDYR
                     SNNKYVTSFLLMPTVIVVNSDLQGDIKIRGYQDLLQPILKGKIAYSNPNTTTTGYQHM
                     RAIYSMHHRVSDVHQFQNHAMQLSKTSKVIEDVAKGKYYAGLSYEQDARTWKNKGYPV
                     SIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVAEFDAKSIRKDVS
                     EQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
BASE COUNT         2247 a         1084 c         1293 g         2426 t
ORIGIN      
        1 aaagaaggag tgcttttcaa tgaaaatagg tgtattttca gtattatttt acgataaaaa
       61 ttttgaagat atgttagatt atgtcgcaga atctggattg gatatgattg aagttggaac
      121 aggtggtaac ccaggagata aattttgtaa gttagatgag ttgttagaaa atgaagacaa
      181 gcgacaagca tttatgaagt caatcacaga cagaggctta caaataagtg gtttcagttg
      241 tcataacaat ccaatttctc cagatccgat agaagcgaaa gaagccgatg aaacgttacg
      301 taaaacaatc cgtttagcaa atctattaga cgtgccagtt gttaatacat tttctggcat
      361 tgcagggtca gatgataccg ctaaaaagcc taattggcct gttacacctt ggccaacagc
      421 ctactctgaa atttatgatt atcagtggaa tgaaaagttg ataccatatt ggcaagattt
      481 agctgagttt gcaaaagagc aagatgtaaa aattgccata gagttacatg caggattttt
      541 agttcataca ccatatacga tgttaaagtt acgtgaggct acaaatgaat atatcggtgc
      601 taacttagat cctagtcatt tatggtggca aggtattgat ccaattgctg cgattcgcat
      661 attaggccaa gcaaatgcaa ttcatcactt ccatgctaaa gatacgtata ttaatcaaga
      721 aaatgtaaat atgtatggtc taactgatat gcaaccatat ggtaacgttg cgacaagagc
      781 atggacattc cgtacagttg gttatggaca tagtccatat gtatgggcag atatcataag
      841 tcaacttatt attaatggat atgattatgt attaagtatt gaacatgaag atcctattat
      901 gtcagtagaa gaaggtttcc aaaaagcttg tcaaactttg aaatctgtta atatttacga
      961 caagccagca gacatgtggt gggcataata cgaactcgag gttagtctga agtttgtctg
     1021 aagtaagact ggtggcagtg ttgaataaat gcatatgtcg ccaagccatt gccaaaaatt
     1081 tcacacctta aatcaagtca ttgtttgtaa agaaggtgta ctttatataa gtatatagcg
     1141 atggtcatac ccattcacag taacaatcct caccattgaa aagagtatat aaccttttca
     1201 atagtgaggt atatgataat aaaaaaagcc tgttgtcaca atggtcatag acacgacata
     1261 ctttaaaggt ttctgaatat aatatttcag attgcacttt aaagatggac gtcgatgtag
     1321 actaatgtga tgacaggctt tcatcttttt aaatattcat taatttctct tcttgtttaa
     1381 tacgtacata taagaaatac gcatacggta ctaatagaat agttgtatat gttgcgtgtg
     1441 ttaataataa tacaccgatt aattcaggaa tgatgtttaa gaagtaattt gggtgttttg
     1501 taattttata taatccagat ttaataatag gatggttagg taaaatgaat aattttaatg
     1561 tccaaatacc acctaaagtt ttaataacca taaataacat gatataagca aagattaata
     1621 taactaagcc aataccattt gcaaagctaa atgtatcttt attaataaat gcctctacac
     1681 cagccaatac ataaattaaa acgtgtgtta ttgctaaaaa cttcgaattt ttaacgccat
     1741 attcaactgc accgtctgct tttaattgtt ttgagtgatt aatagatatc tttaagctga
     1801 caagtctgat acagaaaaag ataagtaata tagatagaat catgatgtcc tccgtcatta
     1861 tgtcatatgt ataagcgttg attttgacaa cataaagtat tttatagata aaacttgtca
     1921 aatactatta actatttatt aattttagta cataaatatg tttctaagta tgtgtttatg
     1981 ttcagtattt tggataattt aataatttta aggatattaa gcgcttacac cgacgtgata
     2041 tatttggctt aacgaaaatg attgaggtga cagagatgaa cttttttgat atccataaga
     2101 ttccgaacaa aggcattcca ttatcggtac aacgtaaatt atggcttaga aacttcatgc
     2161 aagctttctt tgtagtgttc tttgtttata tggctatgta tttaattcga aacaacttta
     2221 aggcggcaca accgttttta aaagaggaaa ttggattatc tacattagaa cttggttata
     2281 tcggattagc atttagtatc acgtacggtt tagggaaaac attacttgga tattttgtcg
     2341 atggacgtaa cacaaaacgt attatctcat tcttacttat cttatctgcg attacagttt
     2401 taattatggg atttgtgtta agttactttg gttctgtaat gggattatta attgtacttt
     2461 ggggacttaa cggggtgttc caatcagttg gtggacctgc aagttattca acgatttcaa
     2521 gatgggcgcc aagaacgaaa cgtggccgat acttaggatt ctggaataca tcacataata
     2581 tcggtggtgc cattgcaggt ggtgttgcac tttggggtgc taatgtattc ttccatggaa
     2641 atgttatagg gatgttcatt ttcccatcgg tgattgcatt acttattggt atcgcaacat
     2701 tatttatcgg aaaagatgat ccagaagaat taggatggaa tcgtgctgaa gaaatttggg
     2761 aagagccggt cgataaagaa aatattgatt ctcaaggtat gacgaaatgg gagatcttta
     2821 aaaaatatat cctgggaaat cctgttatat ggattctatg tgtttcaaac gtctttgtat
     2881 acattgtacg aatcggtatt gataactggg caccgttata tgtgtcagag catttacact
     2941 ttagtaaagg cgatgcagtt aatacgatat tctactttga aattggtgct ttagttgcaa
     3001 gtttattatg gggctacgta tcagacttat taaaaggtcg tcgtgcaatt gtagctattg
     3061 gctgtttgtt tatgattaca tttgttgtct tattctacac aaatgctaca agtgtcatga
     3121 tggttaacat ttcattgttt gcattaggtg cgttaatctt tggtccgcaa ttattaattg
     3181 gtgtatcatt gactggtttt gttcctaaaa atgccatcag tgtagcaaac ggaatgacag
     3241 gttcattcgc gtatctattc ggtgactcaa tggcgaaagt tggtttggcg gctattgctg
     3301 atccaacacg taacggttta aacatctttg gatatacatt aagtggatgg acagatgttt
     3361 tcatcgtctt ctatgttgca ttattcctag gcatgattct attaggaatc gttgctttct
     3421 atgaagaaaa gaaaattaga agtttaaaaa tttaatataa atcggattaa aagtatcgcc
     3481 aatctattgc aatatagttg gcaatcctgc cccgacggca tgtgcgtgaa gagatgaaag
     3541 atactgcttc tacccttgca aatatatcat ctctatgtct cggggcagat cataattccc
     3601 tgttatgaag tatccttatt tgcccgactt agggtgactc aatgaattta ctccttacaa
     3661 taaagacata tagcggtgtc aatattgtag ggagtattgt tttatattta aactctctaa
     3721 aaagcggact gaaagaaaag tgaaaacttc tctatcagtc cgctttttca tagaacaaaa
     3781 tgtaggcgcc ataatcatta gttatttgct aatctatttt gcttgcttac aataatcact
     3841 tggagacatt tgtaaatatt ttttaaaatg atagctaaac attttatact ctgaaaagcc
     3901 tactttgtct gcaatttcat agtgtttata atgtcgatct aacaattgca gagattgtaa
     3961 aatacgatag cgatttaaat aatcgacaat tgtaatacca acatgatctt taaatgttcg
     4021 catcgcatac gattcactaa catcgatatg ttgaattaaa tctgaaacag tcactttcgt
     4081 ttgataagat tgcttaattt gatcaacaat ctggtttaca taataatcat cgtattctac
     4141 ttttaatagt ggttggaagg catcatgaca aggtgctaag ctacggccgt tctgtgattg
     4201 ttgctctaat aaggtacgga caagtcttcc taaaataact tctaattgtg catggtctac
     4261 tggttttaat aaataatcaa gaacatgatg ttgaatgccg gctttcatat attcaaagtc
     4321 atcgtaactc gataatatga tgacattaca atctagatgc gcaatatcat tgagtaaatc
     4381 gacgccattt ttacgtggca tacgaatatc agtaattact aattctggct gatgttgttg
     4441 aattagtgat aatgcttcaa caccatcttt agcagtgtat attgtattga aatgatagtc
     4501 tccccaagga atgatttgct ttaaaccttc tcgaataatt cgttcatcat cacaaataac
     4561 taccttaaac atctacattc ccccttgaaa gtggtatttt ataacaaatt aacgtacctt
     4621 gattacgctt tgaaaaaata tggagtcgtg catgtgaacc atattgaatc attgctttat
     4681 tgtgtaaatg atttaatccc aaatgcttag tatcaaatac atcattatta agagattggc
     4741 gtacatattg caggcgagat gacgacatcc caataccatt gtcgcaaact aaaacatgta
     4801 aattctgacg tgccaatgtc aggcgtatag taatatccaa tgactcagta tctctaccat
     4861 gtttaatagc attttctatg agtggttgaa gcatcatttt accaattgtc tggtgacgcg
     4921 cttcttcaga actttcgata tggagcttaa tcatgtcatc aaaacggatg ttttgtatcg
     4981 caacatactg ttcaatgtag ttcaactctt cgtttaattc cactgtatgt gagtttgtac
     5041 gtaatgagta acgtaacatt tgcgataatt gttggaccac agtttgtgct aatttcggag
     5101 ataacgtaat taaatattgt attgtttgca tcgtattgaa taggaaatga ggctggaatt
     5161 ggcgttctat ttcctttaac tgaatatcac gcaagcgacg ttctgtatgc tcgatagaat
     5221 ggatcagttg ctcatttgat tcaaataaat cgtaaatata attattaatt tcttctagtt
     5281 cactgttgtt ttttaaaggc gtatatgtac ctagatgacg atttttggca tagtaaattt
     5341 tttgaataat cgtttcgata tcttttgttt gtcgtttagc catattatct gcgctaatga
     5401 aaccaaatat tactagtaaa acaagaacta cggccataac aattaataac gtgataccat
     5461 cttcaatatt ttcatgtata tctttataaa taatgagacg atggtcagca tggtttaatt
     5521 ttacagattc attcataaat ccgaattgtt gtggtctata cttttcacct atagtaaaac
     5581 ggtcatcatt ggcgtataaa atattgtcat attgatcaac gataagtgcg aattgtcggc
     5641 tatctttctt aatttcactt aaacgtgggg tgttagccat ataaatttta agcatatatg
     5701 tactattttt gaatttaagc tgatgcgttg aaaataaata catattttta gtgtttaaat
     5761 gttcataatt attggttata aactgatttg gtccagataa ttcataataa agtgttgcgg
     5821 gctgttggtg tattaatttt aataattcac gttttgtagc ggtaacatca tgatgatttg
     5881 ttaaatcgag ctcttgaaac gaattattat gctgtgtaat aaatgtctga atctgctttt
     5941 cagtatgatg taaagatgac tgactttcat caacatgttg atgaatcgta cgatgctcaa
     6001 tccaaatata gatggcatag aagcttacta gtccaataat aatgactaaa aatactggaa
     6061 aaatagtaga cgcaaataac gatcgtctca attgatgtct ataaggtttg tatgccgtca
     6121 ttgaatcatc tccaaaaatt tatgatgtgg aatgtccggt aatttagatt tcggtattaa
     6181 aggtatgttc ttaagatttt cgatagactg atcgctttgt tcactaacat cctttcgaat
     6241 tgacttggca tcgaactctg caactaatcg ttgttgtact gagcggcttg ttaaatattg
     6301 cactaacttt ttacgcttag gatgagggtg tgcattttta actaaagcaa taccatcaac
     6361 atttaacatt gttccttcaa ttggataaac gattgataca ggataacctt tgtttttcca
     6421 tgtgcgtgca tcttgttcgt agcttagacc tgcgtaatat ttaccttttg caacatcttc
     6481 aatgacttta gacgtctttg acagttgcat cgcatggttt tggaattgat gcacatcact
     6541 tactcgatga tgcatgctat aaatagcacg catatgttga tagcctgtcg ttgttgtatt
     6601 tggatttgag tacgcaattt tacctttaag tataggttgt aataaatctt gataacctcg
     6661 aatcttaata tctccttgta aatctgaatt aactactata actgttggca ttaatagaaa
     6721 actagtaaca tatttattgt tcgagcgata atcctctaat tgctgtgtta cagatgtatc
     6781 ttgataggga acaaaatctt ctggatgatc aattgtttct gacaacacac cacccataaa
     6841 gacatcacca cgctccgaaa aatcttcatt atgcaagttt gaaagcagta cttgagtaga
     6901 tccgtgttta atttcaattt tgacatgctc ttgtttttca aattcattta aaattggacg
     6961 aatcaagttt gattgatacg gagaataaac tgttaataca tttttatctg attcagagtg
     7021 acgcgtatta gcgcatgctg ataaaaaaat
//
LOCUS       DAHHYJ010000128         7027 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000128 DAHHYJ010000000
VERSION     DAHHYJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.128"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            273..1184
                     /gene="rapZ"
                     /locus_tag="NBK22_002148"
     CDS             273..1184
                     /gene="rapZ"
                     /locus_tag="NBK22_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCD2415325.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1181..2176
                     /locus_tag="NBK22_002149"
     CDS             1181..2176
                     /locus_tag="NBK22_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCD2415326.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2287..3231
                     /gene="whiA"
                     /locus_tag="NBK22_002150"
     CDS             2287..3231
                     /gene="whiA"
                     /locus_tag="NBK22_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCD2415327.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3805..4392
                     /gene="clpP"
                     /locus_tag="NBK22_002151"
     CDS             3805..4392
                     /gene="clpP"
                     /locus_tag="NBK22_002151"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCD2415328.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            complement(4635..5537)
                     /locus_tag="NBK22_002152"
     CDS             complement(4635..5537)
                     /locus_tag="NBK22_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCD2415329.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGMILSNEGGALQTMKLPYKYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLIKE"
     gene            6240..6869
                     /locus_tag="NBK22_002153"
     CDS             6240..6869
                     /locus_tag="NBK22_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCD2415330.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
BASE COUNT         2635 a          968 c         1272 g         2152 t
ORIGIN      
        1 tagaatttct tttcgaaatt ctctgtgttg gggcccctga ctagagttga aaaaagcttg
       61 ttgcaagcgc attttcattc agtcaactac tagcaatata atattataga ccctaggaca
      121 ttgatttatg tcccaagctc cttttaaatg atgtatattt ttagaaattt aatctagaca
      181 tagttggaaa taaatataaa acatcgttgc ttaattttgt catagaacat ttaaattaac
      241 atcatgaaat tcgttttggc ggtgaaaaaa taatggataa taatgaaaaa gaaaaaagta
      301 aaagtgaact attagttgta acaggtttat ctggcgcagg taaatctttg gttattcaat
      361 gtttagaaga catgggatat ttttgtgtag ataatctacc accagtgtta ttgcctaaat
      421 ttgtagagtt gatggaacaa ggaaatccat ccttaagaaa agtggcaatt gcaattgatt
      481 taagaggtaa ggaactattt aattcattag ttgcagtagt ggataaagtc aaaagtgaaa
      541 gtgacgtcat cattgatgtt atgtttttag aagcaagtac tgaaaaatta atttcaagat
      601 ataaggaaac gcgtcgtgca catcctttga tggaacaagg taaaagatcg ttaatcaatg
      661 caattaatga tgagcgagag catttgtctc aaattagaag tatagctaat tttgttatag
      721 atactacaaa gttatcacct aaagaattaa aagaacgcat tcgtcgatac tatgaagatg
      781 aagagtttga aacttttaca attaatgtca caagtttcgg ttttaaacat gggattcaga
      841 tggatgcaga tttagtattt gatgtacgat ttttaccaaa tccatattat gtagtagatt
      901 taagaccttt aacaggatta gataaagacg tttataatta tgttatgaaa tggaaagaga
      961 cggagatttt ctttgaaaaa ttaactgatt tgttagattt tatgataccc gggtataaaa
     1021 aagaagggaa atctcaatta gtaattgcca tcggttgtac gggtggacaa catcgatctg
     1081 tagcattagc agaacgacta ggtaattatc taaatgaagt atttgaatat aatgtttatg
     1141 tgcatcatag ggacgcacat attgaaagtg gcgagaaaaa atgagacaaa taaaagttgt
     1201 acttatcggt ggtggcactg gcttatcagt tatggctagg ggattaagag aattcccaat
     1261 tgatattacg gcgattgtaa cagttgctga taatggtggg agtacaggga aaatcagaga
     1321 tgaaatggat ataccagcac caggagacat cagaaatgtg attgcagctt taagtgattc
     1381 tgagtcagtt ttaagccaac tttttcagta tcgctttgaa gaaaatcaaa ttagcggtca
     1441 ctcattaggt aatttattaa tcgcaggtat gactaatatt acgaatgatt tcggacatgc
     1501 cattaaagca ttaagtaaaa ttttaaatat taaaggtaga gtcattccat ctacaaatac
     1561 aagtgtgcaa ttaaatgctg ttatggaaga tggagaaatt gtttttggag aaacaaatat
     1621 tcctaaaaaa cataaaaaaa ttgatcgtgt gtttttagaa cctaacgatg tgcaaccaat
     1681 ggaagaagca atcgatgctt taagggaagc agatttaatc gttcttggac cagggtcatt
     1741 atatacgagc gttatttcta acttatgtgt caatggtatt tcagatgcgt taattcattc
     1801 tgatgcgcct aagctatatg tttctaatgt gatgacgcaa cctggggaaa cagatggtta
     1861 tagcgtgaaa gatcatatcg atgcgattca tagacaagct ggacaaccgt ttattgatta
     1921 tgtcatttgt agtacacaaa ctttcaatgc tcaagttttg aaaaaatatg aagaaaaaca
     1981 ttctaaacca gttgaagtta ataaggctga acttgaaaaa gaaagcataa atgtaaaaac
     2041 atcttcaaat ttagttgaaa tttctgaaaa tcatttagta agacataata ctaaagtgtt
     2101 atcgacaatg atttatgaca tagctttaga attaattagt actattcctt tcgtaccaag
     2161 tgataaacgt aaataatata gaacgtaatc atattatgat atgataatag agctgtgtaa
     2221 aaaaatgaaa atagacagtg gttctaaggt gaatcatgtt ttaaataaga aaggaatgac
     2281 tgtacgatga gctttgcatc agaaatgaaa aatgaattaa ctagaataga cgtcgatgaa
     2341 atgaatgcaa aagcagagct cagtgcactg attcgaatga atggtgcact tagtctttca
     2401 aatcaacaat ttgttataaa tgttcaaacg gaaaatgcaa caacggcaag acgtatttat
     2461 tcgttgatta aacgtgtctt taatgtggaa gttgaaatat tagtccgtaa aaaaatgaaa
     2521 cttaaaaaaa ataatattta tatttgtcgt acaaagatga aagcgaaaga aattcttgat
     2581 gaattaggaa ttttaaaaga cggcattttt acgcatgaaa ttgatcattc aatgattcaa
     2641 gatgacgaaa tgagacgcag ttacttgaga ggagcttttc tggcaggtgg ctcagtgaat
     2701 aaccccgaaa catcttcgta ccatttggaa attttttctc aaaatgagag tcatgcagaa
     2761 ggcttaacga aactaatgaa tagttatgag ttgaatgcca aacatttaga gcgaaaaaaa
     2821 ggaagtatta cgtatttaaa agaagcggaa aagatttcgg attttcttag tttgataggt
     2881 ggctatcaag cgttattaaa atttgaagac gtacgtattg taagagatat gcgtaattct
     2941 gttaaccgac tcgttaattg tgaaacggca aatctaaata aaacagttag tgctgcgatg
     3001 aaacaagttg agagcattaa attgattgat aaagaaattg gtattgaaaa tttaccagac
     3061 aggttgagag agattgctag aattcgagta gaacatcaag aaatttcgtt gaaagagctt
     3121 ggagaaatgg tatcaactgg tccaatttca aaatcaggtg taaatcatcg attaagaaaa
     3181 cttaatgact tagccgataa gattagaaat ggtgaacaaa tagaattata agtaagaagg
     3241 tgtttttggt agtgaattat caaaaatacc ttttttatga ttaaaaagtg tttaatgaat
     3301 aatagtcgac taactattaa attgagctgg aaagtttaat gaaggaaatt caataaccaa
     3361 gcgttaccta attttctcat taagtagcat catatttatc aatttggtca taagaaaata
     3421 cgaatgtgat aattaattga gtcactgaaa gtccctgatc atagacaaaa acaaaatcag
     3481 cataaataag aatcccgaca ttgcgggatt ctgtgtattg aaaagtattg tattttatta
     3541 aaacagcctc cttgaaggga attgaacccc tatcttaaga accggaatct tacgtgttat
     3601 ccattacacc acaaggagca aatatgtaat catatctctg tactatgaga agtaaaataa
     3661 gtacaatcta aaatagatat tatcagttta caaaggaaag agaaaagcgt caaacaatgt
     3721 aactatttaa agtcaaagtg tttgaccaaa tttgacttaa tatgtaaaat aatgagtaac
     3781 agttattaca aggaggaaat atagatgaat ttaattccta cagttattga aacaacaaac
     3841 cgcggtgaac gtgcatatga tatatactca cgtttattaa aagaccgtat tattatgtta
     3901 ggttcacaaa ttgatgacaa cgtagcaaat tcaatcgtat cacagttatt attcttacaa
     3961 gcgcaagact cagagaaaga tatttattta tacattaatt caccaggtgg aagtgtaaca
     4021 gctggttttg cgatttatga tacaattcaa cacattaaac ctgatgttca aacaatttgt
     4081 atcggtatgg ctgcatcaat gggatcattc ttattagcag ctggtgcaaa aggtaaacgt
     4141 ttcgcgttac caaatgcaga agtaatgatt caccaaccat taggtggtgc tcaaggacaa
     4201 gcaactgaaa tcgaaattgc tgcaaatcac attttaaaaa cacgtgaaaa attaaaccgc
     4261 attttatcag agcgtactgg tcaaagtatt gaaaaaatac aaaaagacac agatcgtgat
     4321 aacttcttaa ctgcagaaga agctaaagaa tatggcttaa ttgatgaagt gatggtacct
     4381 gaaacaaaat aattcaaagt aaagagtaga ctaagcagtc tgctcttttt gtatgagtaa
     4441 accgaggtgt caataaattg tttactatac tttgagcgga aatatgattg aatgaagcta
     4501 gttgaaccgt aactatatga aatgttccct tcaaagtaga cattgaaagg aacatttcaa
     4561 tcctttgttt gtaagtcgct ctagacatta catttagtac atatgttgtt tctaatgctc
     4621 attaatggta ttgattattc tttgattaaa tcttcaagtg ccatttttaa attactatat
     4681 ttaaattgga atcccaatgc ttgaatttta ttaggtaata ctttttgagt atccaatact
     4741 actgttgaca tttgaccaag tatgagacgc attgcaagac ttggtgccca agtttcatgg
     4801 ggcttatgca tagctcttgc taaagtgtag ccaaataaat tttgacgctc aggtataggt
     4861 gcagttaaat taaacggacc actagctgac tcgttattta ttaaaaataa aatagcttga
     4921 attaaatcat tgatatgaat ccatgaatac cattgttgac cagaacctaa tttaccacca
     4981 atgtaatatt tgtatggtag tttcattgtt tgtaacgcac cgccttcatt cgataaaatc
     5041 ataccgaaac gaccgatgac aactcgcgta cctaattgtt caaattgttg tgcgaaacgt
     5101 tcccattgat acacaatatc tgataagaaa tcaaatggta aagttttata aacttctgtg
     5161 taactcataa ataaatcagg aggatagtaa ccagtggcac tagcattaaa taaaacttta
     5221 ggtgctttat tacgtgattt aaacaattca tataaagctt gcgtagattg aattctactt
     5281 agcattagcg tttgtttata ttccggtgtc catcgtttat tcagtgtagc acctgctaag
     5341 ttgatgacca catcgatatt ttgaggaact ttgtgttccc acccagattt agcccagttg
     5401 acatatgaaa ttttcttatc atttgaaatt tggtcgtgtc gcgttaatat cgtgatatgt
     5461 gaatctgatt ttttaatttc attaactaat tgagatccaa ccataccagt cccaccagta
     5521 attaagtatt gtttcattat cattcacccc atgtaatttt gtatttagtt actatttaat
     5581 aatcattatt gttgttcaaa ggttatacat tatttagaca ataatatgtc aataactttt
     5641 tttgaattgt attttaataa aaatgatata agttataata aaggtgtacc ttcgataacg
     5701 aataaacatc tcttaaaagt atgtgtaaaa cgctgcatga tacaaacgaa ggtaaaaatt
     5761 tgactccctt tagtagtgga cccgtacgtt aatcgtgcgg gtcgttttta tttatttctt
     5821 attcccatta tacatcaatt taaagcatta attttttaaa caaatttaag aatacatagt
     5881 aatataacaa tctaaacata aaaactttta acacaacact taaaccaatg ctttaatttt
     5941 caatacgtag ctataatttg ttgtaaaatc aaaaaggtta aaatgttaat tttcaaaaaa
     6001 aggctcaaaa tatgtttgat ttagttatta aatgttaaga tatataagac tactatttct
     6061 ttgtaaaaat gaatccgatt tacgagtgag taatagtgaa tacagtttaa agttgaagaa
     6121 ggcaaaaaga gtaaatgttt attaatattt gtagaaacta ggtaagcaaa ttagttgtga
     6181 aaatgttaat ggttgcgtga taatttctat atttaaatta gtttgaagtg agggagagta
     6241 tgtcgaatca aaattacgac tacaataaaa atgaagatgg aagtaagaag aaaatgagta
     6301 caacagcgaa agtagttagc attgcgacgg tattgctatt actcggagga ttagtatttg
     6361 caatttttgc atatgtagat cattcgaata aagctaaaga acgtatgttg aacgaacaaa
     6421 agcaggaaca aaaagaaaag cgtcaaaaag aaaatgcaga aaaagagaga aagaaaaagc
     6481 aacaagagga aaaagagcag aatgagctag attcacaagc aaaccaatat cagcaattgc
     6541 cacagcagaa tcaatatcaa tatgtgccac ctcagcaaca agcacctaca aagcaacgtc
     6601 ctgctaaaga agagaatgat gataaagcat caaaggatga gtcgaaagat aaggatgaca
     6661 aagcatctca agataaatca gatgataatc agaagaaaac tgatgataat aaacaaccag
     6721 ctcagcctaa accacagccg caacaaccaa caccaaagcc aaataataat caacaaaaca
     6781 atcaatcaaa tcagcaagca aaaccacaag caccacaaca aaatagccaa tcaacaacaa
     6841 ataaacaaaa taatgctaat gataagtagt atttagtcaa acaaaaatga accagtatga
     6901 cagacaacac aattaattag gttgtctcga atattggttc ttatttttat aattgttaat
     6961 tagaggagag atgatactta aatagttagt tgtttatttt acggatagtg aaatttattt
     7021 tgagtga
//
LOCUS       DAHHYJ010000129         7002 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000129 DAHHYJ010000000
VERSION     DAHHYJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.129"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            178..1116
                     /locus_tag="NBK22_002154"
     CDS             178..1116
                     /locus_tag="NBK22_002154"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCD2415331.1"
                     /translation="MANIVFKLSDKDITTLMSRITFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMLQGNHAEAVSKELLPQHSQLNTNKTKKKNMANSFLEQTLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVPILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYNHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(1218..3620)
                     /locus_tag="NBK22_002155"
     CDS             complement(1218..3620)
                     /locus_tag="NBK22_002155"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCD2415332.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYVPKTFESGIYVFSEAQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPETTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     QTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGEFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVLVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFDKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFSMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3620..4678)
                     /gene="pheS"
                     /locus_tag="NBK22_002156"
     CDS             complement(3620..4678)
                     /gene="pheS"
                     /locus_tag="NBK22_002156"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCD2415333.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEDKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5059..5799)
                     /locus_tag="NBK22_002157"
     CDS             complement(5059..5799)
                     /locus_tag="NBK22_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCD2415334.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPVRLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPHYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGIVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(6040..6147)
                     /locus_tag="NBK22_002158"
     CDS             complement(6040..6147)
                     /locus_tag="NBK22_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415335.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHDNNKLLKHSLI"
     gene            complement(6184..6507)
                     /gene="isdG"
                     /locus_tag="NBK22_002159"
     CDS             complement(6184..6507)
                     /gene="isdG"
                     /locus_tag="NBK22_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCD2415336.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAYTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(6526..>7002)
                     /gene="srtB"
                     /locus_tag="NBK22_002160"
     CDS             complement(6526..>7002)
                     /gene="srtB"
                     /locus_tag="NBK22_002160"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCD2415337.1"
                     /translation="LQGKTNHDYLNLDFEREHRRKGSIFMDFRNELKNLNHNTILYGH
                     HVGDNTMFDVLEDYLKQSFYEKHKIIEFDNKYGKYQLQVFSAYKTTTKDNYIRTDFEN
                     DQDYQQFLDETKRKSVINSDVNVTVKDRIMTLSTCEDAYSETTKRIVVVAKIIKVS"
BASE COUNT         2252 a         1349 c          939 g         2462 t
ORIGIN      
        1 atgtttaaat tgtgtcacta taacattttc ataaacatta tacatgacca ctatgtattt
       61 tgtaagtatc cgcaattaat tctttacaac atacataaat gtttctgacg ttattatcat
      121 ttatgatatg attatttttg ctaaagataa tgaaatttta tgaaaggatt tacacaaatg
      181 gcgaatatcg tttttaaatt gtcggataaa gacataacga cattaatgtc acgcattact
      241 tttgatactg agaatttacc tcaaggtatg aaagcacgtg caaagtatca aaatacaact
      301 gtaaatattt accaatcagg taaagtcatg cttcaaggta atcatgcaga agctgtgtct
      361 aaagaacttc taccgcagca ttctcaattg aatactaata aaactaaaaa gaaaaatatg
      421 gctaattctt ttcttgaaca aactttaatg tatgatcaat ttaactgtat tggtagtgat
      481 gaagctggaa gtggcgatta ctttggtcca ttgactgtat gtgctgcttt cgttaccaaa
      541 gagcatgtcc ctattttaaa aactttgggt gtagatgatt caaaaaaatt aaccgatacc
      601 aaaattgtag aacttgctga gcaattggtt actttcatcc cacattcatt attaacacta
      661 cacaatgaaa aatataatat tcagcaagca aaaggctgga ctcaagtaaa aatgaaagct
      721 gttcttcata atgaagccat caaaaatgta cttgaaaaaa ttgattcgtc tcaattagac
      781 tatatcgtca ttgatcagtt cgcaaaacgc gaagtgtata accattacgc attatccgat
      841 ataccacttc caaagaaaac aaaatttgaa acaaaaggag aatcgaagtc attagccatt
      901 gcagtggcaa gcataatctc tcgatacgca tttgttacat atatggatca aatatcaaaa
      961 aatattaata tgacgattcc taaaggtgcc ggtgctaaag tcgatgtaat cgccgccaaa
     1021 attataaaaa aatacggtct ttcacgttta gataccattt caaaaaagca ttttaaaaat
     1081 cgtgaaaaag cccaaaaaat tttaaagcct ctttgatttt taaatgaatc gaaacctaat
     1141 tggtgaaccc aaaaataaaa ccccatgtta gggatatcaa tctacttcag atatccttat
     1201 acatggggtt taaatcatta tctaataaca gcaccttgtt caattaatgc tgcttcaatt
     1261 tccgcttgca cttttgaaac gcgctcatct gtcaatgttt cttctgtgtc taaataattt
     1321 aaacgtattg caattgattt tttacctttt tctaaatgtt cgccctgata tacatcaaag
     1381 acaagtgtat cttttaatat attgccaccg tgtgcatgaa tcgttgataa taaatcagct
     1441 gctggaatat tttgatctac ttctaatgca atgtcacgag acatgcctgg gaatctcgga
     1501 attggctggt aattaatgta acctaccgac acagccatta atgcatcaaa attcaactca
     1561 aaaacatacg tacgttttaa atcattatca gctgctaatg ttggatgtaa ttcaccaata
     1621 aaaccaacaa ctttattctc taataagatt tcagcagtac gacctggatg taatccatca
     1681 atatcagcac gacgataact aaattcaaga tttaactttt cagatactcg atccacgaca
     1741 ccttttgcta aatagaaatc aaccgtttct ttcttacctt gccattgatt gactacataa
     1801 tctccagtta aaataccact taaatattca acttgatctg gtagttcacc ttctccatta
     1861 gcaaagaaga cattgccgat ttcaaataat tttacatctt tatttttgcg tgccacatta
     1921 tatgatgctg cttcgattaa atgtggtaat aaactttgac gtaatgacgc atgcgcttca
     1981 ctcattggca tcaataaatc aattgtttga cgctgttgca tcgaaaatgc agtagcatcc
     2041 tctttagaaa ctaacgaata cgtaatagct tgatctaatc cagcaccttc taacacttct
     2101 ttaaccattc tagttttata ttggcgatca gttagctgac cactagtaac tttatcgaag
     2161 acaggtaacg ttgatggaat atcgtcgtat ccatatatac gtgcaacttc ttcaattaaa
     2221 tcttctttaa ttgtaatatc tttacgacgt gatggtacta gcactgtaat aacatcatca
     2281 tttatttctg tatcaaaccc tagttgatta aaaatagtaa caatatcatt ttgtgacaaa
     2341 tcaaatccaa tagtgcgatt aattttatca gcagtgatgt cgattggtgt aataaattca
     2401 ccaagttctc ctgaagacac tctatctttt agcacttttc cgtttgcata agtttgtaat
     2461 aaataacatg cacggtcgac tgcttcatct acaaattcag tagctattcc tttttcaaaa
     2521 cgactagatg attcactgcg taaatttaaa cgtcttgatg tatgacgaat tgaaactgga
     2581 tcaaaaatag caccttcaat cactatattt gatgtttgtt ctttaacttc tgaaaaatcg
     2641 ccacccataa caccagctaa tgcaattgga gtttgtccat tagtaatgac aatatcgctc
     2701 gttaataatt cacgttctgt atcatctaat gttgtcattt tttcgccttc attagcttga
     2761 cgaacaacaa tttgttgtga accaatcgca tcttgatcaa acatgtgcaa tggttgaccg
     2821 tattctaata acacataatt tgaaatgtca acaacattat taataggacg tatacccgct
     2881 tttattaagc gtgcttgcat ccaaattggc gaaggttcaa ttgtcacgtc gtgaacaaca
     2941 cgtgcactat aatatggtac tttatcttca ttttcaattg tcacagtcaa ttcatcattt
     3001 gcagataact caagctcatt tgatgttgtc tctggcttag tcatttttgt attatataat
     3061 gctgcaactt cataagcagt acctatcata cttaaagcat cagcacgatt cggcgttaaa
     3121 tcaaattcca ttacttgatc atctaaatat aaagcttgta aggcatctgt tcctggaact
     3181 tgggcttcac taaatacata gataccagat tcaaacgttt ttggtacata attacttgaa
     3241 ataccaattt cttgtaacga acaaatcata ccttctgaac gttcaccgcg taatttggca
     3301 cgcttaattt taataccacc aggcaatctg ccacctactt tagcaacaat gacatattgt
     3361 cctgcatcaa cgttcggtgc accacatacg atttgtacag gttcgtcttc tccaatatca
     3421 acttggcaaa catttaattt atctgcatca ggatgtttct cttttgactt aacgaagccg
     3481 acaactaaat ttttgatatc ttttgtgtag tcaattaaat catccacttc aatacctgtg
     3541 cgcgtaatac gttctgccaa attacttaca gaatcatcga ttgttacata ttctttcaac
     3601 cattcatttg atatcaacat tacatgtcac ctctatcttc taccgcttta aattgatcta
     3661 aaaatctcac atcattagta tagaaatgac gaatatcttc tataccatat ttcaacattg
     3721 caatacggtc tggtcccata ccaaatgcaa atcctgagta ctctgaagaa tcaaaaccag
     3781 ccatttctaa tacattagga tgtaccattc cagcacctaa aatttcaatc catcctgtgt
     3841 gtttacacac attacaacct tttcctttac atttaaaaca tgacacatca acttctacag
     3901 aaggttcagt aaatgggaag taacttgggc gtaaacgaat ttcacgatca gcaccaaata
     3961 atttcttagc taacaattct aaagtacctt tcaaatcact cattttaacg tttttatcaa
     4021 caactaatcc ttcgatttgt gtaaattgat gactatgtgt cgcatcatca gagtcacgac
     4081 gatacacttt accagggcaa ataattttaa ctggaccttg accatgacgt gattccatcg
     4141 tacgtgcctg cactggtgat gtatgcgtac gtaataaaat ttcatccgta atatagaaac
     4201 tatcttgcat atcacgtgca gggtgtgatt taggtaaatt cagcatttcg aagttataat
     4261 gatcttgttc aacttcatat ccattcacaa tttcataacc taaacctaag aataagtctt
     4321 caatttcttc tattgtacgt gttaatggat gctttgaacc gatttcaata tgacgacctg
     4381 gtaatgatac atcaattgtt tcttcagcca attgcttatt taatttttct ttaactaaca
     4441 tctgttgtct ttcatctaat tcattttgaa ttgtttgacg caattcattc actttttgac
     4501 caaacgcagg tttatcttca ttcggcaaat ccttcatcaa tttcattagt ccgctaactg
     4561 accctttttt acctaagtat ttcactttaa cttcttgcag tgcacgttca tcatttgctt
     4621 cattaatatc tacaagcgct tgttgtttta actctgacat tgtttgttgt tcagacatat
     4681 gtcactgacc tccctattcg aactattcat tttctattga tcatatttgt tgtttaacta
     4741 aaaaaagact ttcatcctct taaaaaataa gggacgaaag tcatttccgt ggtaccaccc
     4801 aaatttatgc ctaagcatac acttgtttaa tattgataac ggattgtctt tccgacttaa
     4861 atctctttaa gtaaccaaaa aggtgaaaag actttaaata attggaataa cttgcagtca
     4921 cgattattct ccctaaaaca attatttata taaatgtcca tgccttttta ttggtttata
     4981 ttcaattgtg tatgcattca cgtagaaatg ttctgttaaa caattaatta taattatatc
     5041 ggaaaacttt caacacggtc aacctttcaa atgataaagt aaaatactac ctgcaatcgc
     5101 tacatttaaa ctttcagctt taccataaat aggtatgatt aaattttgtg tagtatgtgc
     5161 taataattca ggattaacac cttctccctc attacctaat agtaatgcaa aagaatcact
     5221 tgaagtaact tctttgtatg ccactgcgtt ttcaagtgct gtaccataaa caggaccatt
     5281 aaattgagta ataaacgtat cgagatcttg tgtcataact ggcaaatgga aaacaatacc
     5341 ttgactcgct cgcaacactt tatcttgata aggatctgtc gtacccttct ccattattac
     5401 agcatccatt ccagcagcat ccgcagttct aattaatgtg cctaaatttc caggatcttg
     5461 aacacgatcg atgagcaata cttgttgtgc tttagaaata tcataatgcg gcttctcaat
     5521 gattgcgaaa aacccttgtg gtgtcactgt acctgataaa gattcagcaa ctttcatgtt
     5581 tatttcaaaa acttcttgcg cgtatgcgat aatttgctga tctaatctta ccggttcaat
     5641 tgcaaataat tgtgtaatta caattccact ttgataagct tcttcaatta aatgcacacc
     5701 ttcaattaaa gctaatccag ttttatccct ctcacgtttc ttttttagct tgttcgcttg
     5761 tttaattcta ttattttgtg cagaagtaat ttgttccatt gatagctcct cgctttattt
     5821 ttaaaaataa aaatattaat cattaataag atgaaaacat ttgattgtat agttaatatt
     5881 aattaatcgc ttttatcact cactaatatt tcaaattgta taaatttctt ttatcgatac
     5941 tactactata aatcattcgc cccaaaatat cattattaat tcttttcttc ttcaaaataa
     6001 atcaaaatca tatatttgat gattattttc aaagcacctt caaatcaaac tatgttttag
     6061 caatttgttg ttatcatgtt tgtgttcatt aaaaaaacga ccatcatcag tatcatgtat
     6121 ggtcgttaca aaagctaaca ataccaattg tcataacaag tactgcaacc tctttaaatt
     6181 caattatttc atgtaactat agcctatatc atatgtaatt actttgttat tgataatcgg
     6241 gctactttca tcttcatttt tacttctaac atgtttatgc gctgctttaa agacatcaga
     6301 ttttaaccaa tccgtataag cttgctttga tttccaaact gttaaaattt tcacttcatc
     6361 aaaatcttct tgttctaaag tttgtgtaac aaacatgcca tcaaagcctt ctaatgtttc
     6421 aatcccatgt ctcgtgtaaa atcgttctat aatatctttt gctgttcctt ttgttaacgt
     6481 cagcctattt tctgccataa atttcataat tatcctcttt tctgtttaac ttaccttaat
     6541 tatttttgcg acaacaacaa ttcttttcgt cgtttcacta tatgcatctt cgcacgttga
     6601 taaagtcatt attctatctt ttaccgttac attaacatct gaattaatta cagatttacg
     6661 ttttgtctca tctaaaaatt gttgataatc ttgatcattt tcaaaatctg tacgtatgta
     6721 attatcttta gtagtagttt tatatgcact aaatacttgc aattgatatt taccatattt
     6781 attgtcaaat tcaattatct tgtgtttttc ataaaacgat tgctttaaat aatcttctaa
     6841 cacatcaaac atcgtattat caccgacatg gtgcccgtat aaaatagtat tatgatttaa
     6901 attcttcaat tcatttctaa aatccataaa aatactacct ttacgtcgat gttctcgctc
     6961 aaaatctaaa tttaaataat cgtgatttgt cttaccttgt ag
//
LOCUS       DAHHYJ010000130         6948 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000130 DAHHYJ010000000
VERSION     DAHHYJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.130"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            102..893
                     /locus_tag="NBK22_002161"
     CDS             102..893
                     /locus_tag="NBK22_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCD2415338.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            907..2793
                     /locus_tag="NBK22_002162"
     CDS             907..2793
                     /locus_tag="NBK22_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HCD2415339.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRVSYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDVVVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            3018..4361
                     /gene="brnQ"
                     /locus_tag="NBK22_002163"
     CDS             3018..4361
                     /gene="brnQ"
                     /locus_tag="NBK22_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD2415340.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(4434..5570)
                     /locus_tag="NBK22_002164"
     CDS             complement(4434..5570)
                     /locus_tag="NBK22_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCD2415341.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNTTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVNRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVAAQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(5602..6231)
                     /locus_tag="NBK22_002165"
     CDS             complement(5602..6231)
                     /locus_tag="NBK22_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCD2415342.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(6250..6519)
                     /locus_tag="NBK22_002166"
     CDS             complement(6250..6519)
                     /locus_tag="NBK22_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCD2415343.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            6682..>6948
                     /locus_tag="NBK22_002167"
     CDS             6682..>6948
                     /locus_tag="NBK22_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCD2415344.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVL"
BASE COUNT         2311 a         1019 c         1160 g         2458 t
ORIGIN      
        1 agcattgcgt tttatatcag aatagaatag taaactaagg catttggaga acttattcca
       61 tgtcttagtt ttttaattta tttaaaggag aacgttttaa aatggcacta aaacattata
      121 agaattcaga ttcaacagtt ttcaatgatg cgaaggcatt atttgattta aataaaaata
      181 ttttacttaa aggtccaaca ggttcaggga aaacaaagtt ggcagaaaca ttaagtgaag
      241 ttgttgatac acccatgcat caagtcaatt gttctgttga tttagataca gaaagcttat
      301 taggctttaa aacaattaaa acaaatgcgg aaggtcaaca agaaattgtc tttgtagatg
      361 gtccagttat taaagctatg aaagaggggc atattttata tattgatgaa ataaatatgg
      421 ctaaacctga aacattgcct gtattaaatg gggtcttaga ttatcgtcgt caaattacga
      481 atccatacac tggtgaagta atcaaagctg taccaggatt taacgttata gcagcgataa
      541 atgaaggtta tgttggtact ttgccaatga atgaagcact aaaaaatcgc tttgttgtta
      601 ttcacgttga ttatattgac ggggacattt taaaaaatgt gattaaagag caaagtttat
      661 tacaagatga taaacaaatc gaacaaatta ttaagtttaa cgaagattta cgtactatgt
      721 ctaagcaggg acaaatttct gaagaagccg ctagtatccg tgcattatta gacttgtgtg
      781 atttaatcac tgtaatgcca gttgaacgtg caattaaacg tacaattatt gataaattgg
      841 aagatgaacg tgaacaacaa gcaatatata atgctgtaga actaaacttt taaagaggga
      901 taaaacatga gtgatcgttt cataaaattt aacgacgaac agttagatgc caagcaagta
      961 atgatgttgc aagacttagc tcgactctta ttaaaaaatg aacaaacaca agttaaaatc
     1021 caaaaattcc cttactataa tcctgttcaa aatgttttga ttacgagttg gttttggtca
     1081 catcgaccaa gccatatcga aatggctggt ttaaaaacag atgttatgtt agctgcttac
     1141 ggttatcata tgatggatgt acaaattgta aatgaagtag tacaagataa aacttttaaa
     1201 caccctaaat tttatcaaca actttttaaa ttattagaag atatgcgcgt attaaattct
     1261 attaaagttg agcgcccaag tactgctaaa ttaattgatc ttcgcttaga tacacgggta
     1321 tcatatacgg aatctcaaat taaagtgtat cgtactaaaa cacaatatac agatttatta
     1381 tttttgtatt tagaacatgc atttttaagt caagatttct ttgatatacc atctatacat
     1441 tcagatttag atgatgttgt agttaatatg ttcttatatt tacctaattt tttccaaaat
     1501 caaaattctg aagataatat gtatctggct caaagaatta tgtatcaagt agatgatatt
     1561 ttaaaagaag atatgctaaa cgaatattat tacttaccta aaacactata taatacatta
     1621 gctagtccag agtttgatga tttaaagaga actgatgcaa gtcaagtcga tgggcaagat
     1681 gatacttcag aagatgatga taacgaaagt gaaaaagctg actctaaatc tgcagatagt
     1741 gagtcaaaag gtggcgccta tttagaaatg gagcttcacg aaggacaaaa tagtgaaaca
     1801 ttaggtaatg atgaagcacg tgaaggcgat gctacagatg atatgactga tatgatgacc
     1861 aaaaagggca aaggatcaaa cgatacgcta aatcgtgaag aaggcgatgc tgttggtcaa
     1921 agccaagcat ttcaacttga tggtgtgaac aaaaatgtag aaataaaatg gcaaattcct
     1981 gaaattgagc cacaatatgt tttagaatat caagaatcga aacaagacgt acaatatgaa
     2041 atcaaagact taattcaaat tataaagaaa acgattgaac gagaacaacg agatgcacgt
     2101 tttaatttaa caaaagggcg attacaaaag gatttaatta attggtttat cgacgatcaa
     2161 tataaattgt tttacaaaaa acaagattta agtaaatcat ttgatgcgac atttacattg
     2221 ttaattgatg cgtcagcaag tatgcatgat aaaatggctg aaacgaagaa aggtgtagtg
     2281 ttattccacg agacgttaaa agcattgaac attaaacatg aaattttatc attcagtgag
     2341 gatgcctttg attcagacga gcatgcacaa ccaaatatca ttaatgaaat tattaactat
     2401 gattactcaa cctttgaaaa agatggccca cgtattatgg cacttgaacc tcaagatgat
     2461 aatcgtgatg gtgttgctat tagagttgca agtgaacgat taatgcgtcg aaatcaacat
     2521 caacgatttt taatcgtatt ttcagacggc gaaccgtctg catttaatta tagtcaagat
     2581 ggtattattg atacgtatga agctgtagaa atgtcacgta aatttggtat tgaagtattt
     2641 aatgtatttt tgagtcaaga tccaataact gaagatgttg aacaaacaat tcataatatt
     2701 tatggtcaat atgctatttt cgttgaaggt gttgcgcatt tacctggaca tctctctcca
     2761 ttattaaaaa aattactact taaatcttta taaataaatt taaaatatga gtttatatga
     2821 aattgtgaat aaaagtcatc agttactatt taaaaagtgg atttgaagaa ttttattttc
     2881 tgacataatt tgatttaaac aacacatgtg ttttgtttct gctatgtaca acatatttca
     2941 tatagactct ttattatttt ttgtgctaaa atatttactt gtaaattatt tcgtgtttta
     3001 aaaaaaggag taactctatg aataaaaata catgggtcat tgggtttacg ctcttcgcta
     3061 tgttttttgg cgcaggcaat ctaatttttc ctcctaatct cggattggat agtggtcaat
     3121 ttttctggcc cgccatttta gcgtttgttc taactgggat tggtttacca ttattaggtg
     3181 tgattgtagg tgcacttgat aaagaaggat atattggcgc attaaataaa atttcaccta
     3241 aattttcaat attgttctta atcatcattt atttgactat aggaccactt tttgcaatac
     3301 ctagaactgc atctacatct tttgaaatga caattacacc aattatacat agcaatagta
     3361 gtatcgcttt atttatattt acgattatct acttcatagt cgttttgtat atttgtttaa
     3421 atccatctaa gttaatcgat cgtattggtt cattattaac accattatta ttgattacta
     3481 ttttagcgat gattattaaa ggatacttag actttagcgg taatagtgct ggaaagggca
     3541 atgaagcact atatcattct aatttttcaa gttttgctga aggctttaca caaggctatt
     3601 taacaatgga tgccattgca gcaattgctt tttcaatgat tgttgttaat gcagtaaaac
     3661 taacaggcat tactaaaaca aatcaaatat tcaaacaaac tttgactgct ggtttaattg
     3721 cagccgtagc tttaattttc atatatattt cattaggtta tattggtaat catatgccag
     3781 taagtgacat gacgttagat caattgaaat ccaaagatcg aaacattggg acatatttat
     3841 taacgacaat ggcttcaaca ggatttggtt cattcggaaa atatttattg ggcatcattg
     3901 tggcgctggc atgtctaact acagcatgcg ggcttattgt tgcagtttct gaatatttcc
     3961 atagaatcgt acctaaagta tcatacaaag catttgtatt agttttcatt ttaatgagtt
     4021 ttattattgc taaccaaggt ttaaatgctg ttatctcaat gtcaattccg gtattaagca
     4081 ttgtataccc agtagcaata actgttgtat tattaatttt aattgccaaa ttcataccga
     4141 caaaacgcat ttcacaacaa attccagtta ttatcgtatt tatattgtcg attttcagtg
     4201 ttattagtaa gttaggttgg ctgaaaatta actttataga atcattgcct ctaagagcgt
     4261 attctttaga gtggttccca gtagcaatta ttgcaacgat attaggctat ctagtcggca
     4321 tatttgtaaa acaagatcca attaaatatc aacaggaata acgaataata taaaaggaag
     4381 catctccaat ttaatcgtag gatgcttcct ttttgttata tctaattcgt aatttatgat
     4441 tgtttattcg gtcctttgat gttcactaaa tgtgacttta aatcttgttc taattgttgt
     4501 aattcttttt cagctaattg tcgttcttcg cgaccgtgtt gttgaataat taatgtttct
     4561 tcaattgtct caataatgtt acgctgtgta cgtttcaatg tatcaagatc aacaatgcca
     4621 cgctcatttt ctgttgcagt ttcaatcgca ttttgtttca acatttcagc atttgctgtt
     4681 aataaatcat tagttgtatc agtgacagct cgttgtgcag caactgcatt acgctgtctc
     4741 attaatgtaa gcgcaatggc catttgattt ttccatagtg gaatacttgt caaaattgaa
     4801 ctttgtatct tctcggcaag tgcttgatta acattttgaa tcatacgaat ttgtggcgca
     4861 gtttgcaaag ctatttgtct tgaaagctgt aagtcatata tgcgtttatc tagtctatct
     4921 ataaattgct gcatatctgc aacttgttga atatccattt gattagtgga ttgctgcgct
     4981 tgctgttgca attgtggtag cttttcattt tctaattgca actttttttg ctgtgcagca
     5041 atgatatgca atgataagtc atcaaagtat tgtttgtttt tatcatatag cgtatctaat
     5101 aattcaatat ctcttgttaa atgtgtttga tgtttctgca gttgaatcgt tatgcgattg
     5161 acttgagcac taactgattg cattcttgaa aagatttcat tgatagacga ctttgctctg
     5221 ctaaaaattc tttttaacat agatggttta tcagtattta actcatttgg attaactgac
     5281 tttagttttg acattagatc tgacaaagta tctccaatag gaccaacatc tttactttgt
     5341 acttcatcca acatttgatg tgaaaattga gacatttgtt tctgtaaatc agaaccaaac
     5401 gctaataaac cttcattgtc taaagggtta atttgtttac tgattgtgtc tacctgtttt
     5461 tgttgttcaa ttgttaactt ttctttttga atagtagtat tcgataattc tttatcgctt
     5521 ataaaatcat ctagtggatg gctttctttg aaacttttat tttcagtcat caatcatctc
     5581 tccaatagtt gctattttac atcaatcttg atgatgcttt tgatgtaact tatttaattc
     5641 catttcaata tctaaacgtt cataatcatc ttcgttgaga cgctttaaat cagcgattaa
     5701 tgttcgtttg acctcatcca aagtaattcg tgtttgttct aatttttgct gttcattaat
     5761 tgattttttg ggcatttttg ctagacgtgt atatgcatca accaaattta aagcattatc
     5821 aatatgagaa taaaaaaagc cttcaacttt ataaaatgat gcaggtctct gtctaactgt
     5881 cgtataaata gaacgtgaaa tttggtatat atcattaatc tgcctaaaat ctttaattga
     5941 tcttatattg acatacgttt ttaaaatacc tctaagtttt tggtgtgtat gatttaactg
     6001 atttcgaata tagcgatagt cttttctagt caaaccaatt tcgtttaaat atttgcgtga
     6061 agtgagtttt tgtatcggta ggtatgtcat taaaaagcca acaataccaa tagacatatc
     6121 aattaaaaaa gatacatcaa gtgcaatcat cccaaatatg cttgttaaaa acgctacagg
     6181 aattcccact aacaccccaa atatatgaga aatattatat ctcactgtca tcttccttta
     6241 tttagcattt tatattgatc gaaaatccga taatttttga tttagttcta actcttcaag
     6301 ttgatggctt gttacatttg atgctggtga ggcaccttca attacacctt gaataaatct
     6361 ctctatatct gcgtcatccc cttgtgcata tatctctaca tagtcatcta cattttgaac
     6421 agtaccgaca atgttatagt tcattgcaat gcgttgtgta aaatatctaa atccgacgcc
     6481 ttgaacgcgt ccgaatactt gtaaatgtat atgtctcatt tttaccacct cataatgtta
     6541 ttatacgtag ttttacttaa aaaaactaat aattactata gttactactt tgtttgtttc
     6601 aagtcgtcaa acttgatttt cagaggataa aggtataaaa ataagtatag agtttttgaa
     6661 gtatggaagg ggtctttaat aatgtggaca gttaccaaaa ttagagccga ttatgaggga
     6721 tggtggttat tcagtgactg gccagaaaac attgttgaaa aatatcaata tcaagatttt
     6781 gatgacatgt ttaagcacta tcaacaattg attaatcaat gtaaagttca gttcgataac
     6841 tatgtcacag gcaaatataa tatttatgca ttttataata attgtgacat gaactattgc
     6901 gaagattgtg aagaggattt acaaatattt tatagtttca ttgtttta
//
LOCUS       DAHHYJ010000131         6935 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000131 DAHHYJ010000000
VERSION     DAHHYJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.131"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(79..639)
                     /gene="icaR"
                     /locus_tag="NBK22_002168"
     CDS             complement(79..639)
                     /gene="icaR"
                     /locus_tag="NBK22_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCD2415345.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
     gene            803..2041
                     /gene="pgaC"
                     /locus_tag="NBK22_002169"
     CDS             803..2041
                     /gene="pgaC"
                     /locus_tag="NBK22_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCD2415346.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            2005..2310
                     /gene="icaD"
                     /locus_tag="NBK22_002170"
     CDS             2005..2310
                     /gene="icaD"
                     /locus_tag="NBK22_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCD2415347.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            2307..3179
                     /gene="icaB"
                     /locus_tag="NBK22_002171"
     CDS             2307..3179
                     /gene="icaB"
                     /locus_tag="NBK22_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCD2415348.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKASEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNDDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            3166..4222
                     /gene="icaC"
                     /locus_tag="NBK22_002172"
                     /pseudo
     CDS             3166..4222
                     /gene="icaC"
                     /locus_tag="NBK22_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
     gene            complement(4726..6768)
                     /gene="lip1"
                     /locus_tag="NBK22_002173"
     CDS             complement(4726..6768)
                     /gene="lip1"
                     /locus_tag="NBK22_002173"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCD2415349.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSLNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVTSQQQSKEAD
                     NSQDSNANNNLPSQSLTKEAPSLNKSDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFKNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKENDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGNREEIEYQKKHGGEISPLFKGNHDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
BASE COUNT         2161 a         1135 c         1059 g         2580 t
ORIGIN      
        1 ctttatatag atttaaaaaa ataatctcat gtcttttttt acaaaagtaa gttaattatt
       61 acaaactagt aacaaaaatt atttcttcaa aaatatattt agtagcgaat acacttcatc
      121 tttgaattga cttttacttt cttccactgc tccaaatttt tgcgaaaagg atgctttcaa
      181 ataccaactt tcaagaaaca gcaatattaa attctgaaag tcttcttttg tcatctttat
      241 ctttgattca tcatagaatt ttgctatctc tttacttaat gattgattta aatcttgtat
      301 ttgtccgtaa atatttccag aaaattcctc aggcgtatta gataattgaa cgtacattct
      361 aatatacctt tcttcgatgt cgaaaataaa ctcaaataag aattgatata aagcatcaat
      421 tgaatagttc gatttatttt gattcatcat aataatatta ttaaggtaat caaaacaaca
      481 tttaacactt tgttcgtaaa tacttttttt cgagtcaaaa tggtaatata aactcgcttt
      541 ttttatattt acacttttag ctatatcatc aagtgttgta ccgtcatacc ccttctctga
      601 aaataaggtt attgcgttat caataatctt atccttcaat ttttaaaacc ccctactgaa
      661 aattaatcac actatgttac aggaaaatta agttgcaatt acaaatattt ccgtttaatt
      721 ataacaacaa tctattgcaa attaaaatac tatcaattac catatggctt acaacctaac
      781 taacgaaagg taggtaaaga aattgcaatt ttttaacttt ttgctttttt atcctgtatt
      841 tatgtctatt tactggattg tcggttcaat ttatttctat tttaccagag aaattagata
      901 ttcattgaac aagaagcctg acataaatgt ggatgaatta gaaggcatta catttttact
      961 tgcctgttat aacgaaagtg aaacaattga agatacgttg tctaatgttc tggcactcaa
     1021 atacgagaaa aaagaaatta ttatcattaa tgatggaagt tcagataata cagcagaact
     1081 catctataaa attaaagaaa ataatgactt tattttcgtc gatttacaag aaaacagagg
     1141 taaagccaac gcactcaatc aaggcattaa acaggcttca tatgattatg taatgtgctt
     1201 ggatgcagat actatcgttg atcaagatgc accatattat atgattgaga atttcaaaca
     1261 tgatccaaaa cttggtgcag ttacaggtaa tcctagaatt cgaaataaga gttctatttt
     1321 aggtaaaatt caaacgatag aatatgcaag tttaattggc tgtattaagc gaagtcagac
     1381 acttgctggc gcagtcaata ctatttcggg tgtcttcact ctatttaaaa aaagtgcagt
     1441 tgtcgatgtt ggctactggg atactgatat gattaccgaa gatattgcag tttcttggaa
     1501 attgcattta cgtggatatc gtattaagta tgaaccgctt gccatgtgtt ggatgttggt
     1561 tccagaaaca ttgggaggtc tttggaagca acgcgtgaga tgggctcaag ggggacacga
     1621 agtattacta cgagactttt ttagcacaat gaaaacgaaa aggtttcctt tatatatttt
     1681 gatgtttgag caaatcatct cgattttatg ggtatatata gtgcttctat atttaggcta
     1741 tttgttcata acagcaaact tcttagacta tacatttatg acatatagtt tttcaatatt
     1801 tctactatca tcatttacta tgacttttat aaacgttatt caatttacag tcgcactctt
     1861 tattgatagt cgctacgaga aaaagaatat ggctggactc atatttgtaa gttggtatcc
     1921 gacagtatac tggattatta acgcagcagt agttcttgtc gcatttccaa aagcattaaa
     1981 acgtaagaaa ggtggttacg caacatggtc aagcccagac agagggaata cccaacgcta
     2041 aaatcatcgc taaatattgt aagagaaaca gcacttatcg ctatatcgtg tgtcttttgg
     2101 atatattgtt tagttgttct actcgtttat attggtacta tatttgaaat tcatgacgaa
     2161 agtatcaata caatacgtgt tgctttaaac attgaaaata ctgaaatttt agatatattt
     2221 gaaactatgg gcattttcgc gattatcatt tttgtatttt ttacaattag catattgatt
     2281 caaaaatggc agagaggaag agaatcgtga agtatagaaa attaataatt ttagtgttga
     2341 gtatcttgat catattgcct gtaagcacac tggatggtca tcatattgca aatgcagatg
     2401 acgattcacc taaaaaactg aaatataaag aaaatagtgc tctggcatta aattatcacc
     2461 gtgtaagaaa agcgaatttt ctgaataatt tcatttactt cttttctagt agtaaagaaa
     2521 ttaaaaatta tagtgttagt caatcacaat ttgaatctca aataaaatgg ctaaaatcac
     2581 atgatgctaa atttttaacc ttgaaagaat ttttatatta caagaaaaaa ggtaagtttc
     2641 caaaacgaag tgtatggatt aactttgatg atatggatga aactatttat gaaaatgctt
     2701 atccaatctt aaaaaaatat aaaataccgg caactgggtt tattatcaca ggtcatgttg
     2761 gggaagaaaa ctttcacaac ctcgatatga ttagtaaaaa agaactaaaa gaaatgtata
     2821 aaactgggtt atgggaattt gaaacacata cccacgattt gcataactta tctaaaaata
     2881 ataagtcaaa attaatgaaa gcttctgaag ctacaatcat aaaagattta aacaaaagtg
     2941 aaaaatatct aactaaaaac tttaaaaagt cgcagaaaac tatagcctat ccttatggct
     3001 tgatgaatga cgataaatta ccggtaatca aaaaagctgg gttaaaatac ggtttttcat
     3061 tagaggaaaa agcagtcact ccgaactcca atgattatta catccctaga atattaatta
     3121 gtgatgatgc ttttgagcat ttaattaaga gatgggacgg attccatgaa aaagattaga
     3181 cttgaactcg tatatttacg cgctattata tgtgcaatta ttattatcac acatttactt
     3241 acacaaatta ctttaaaaca tgagaatatg gagggaggat ccttagtatt acaattttac
     3301 attcgtaata ttgtgatttt tggtacacct tgctttatta tcttgtcaca gttactgaca
     3361 accttgaatt accaaaaagt cacctataga tacttaacta cacgcgtaaa atatatactt
     3421 attccttaca tattaatggg attgttttac agttatagtg aatcattatt aacagattca
     3481 agtttcaata aacaattcat tgaaaatgtc ctattaggtc aatggtatgg ctattttatc
     3541 gttgttatca tgcaattctt tattttgagt tatatcattt ttaaaattaa ctataaccta
     3601 ttcaacagta aaatattatt attgttatct tttattttac agcaattatt tttatattac
     3661 tttacgaaca acacagcgtt tcacgatacc gtgctacact attatccatt aagtgaaaac
     3721 actataatat tcggatggat tttttatttc tttttaggtg catatatggg ttataactac
     3781 gaacgtgtat taaatttctt agaacgttat ttagttatta tgattgtatt agctgtagct
     3841 acttattttg tgtttattgc gttagcaaat ggagactatt ggaacgttac cagcttttca
     3901 tattcattaa caccatataa tagtattatg tttattgtta tcttgggtat ttgcacgcat
     3961 tttaaaacaa tgttatttaa tacgattcaa atgattagtg ctttctcatt ctttatttat
     4021 ttatttatta catccaatca ttctagactc attgtttgca tatacaaata tatttgagga
     4081 taatacaatg gtctttctag cgatatcact actattcatt ttagggttat gtataggtgt
     4141 cggtatgata ttgcgtgaat tctatatctt taggtttatt attggaaaac aaccatataa
     4201 attgaacatt aatgcttatt aattattaag ctatgttaaa aacacgcggt gggcgaaatc
     4261 agtttgaatt gactgacttc gttttaccgc gtgtttaata atgttataca tatattctaa
     4321 ttgcacattt aaacttcgta aatgccaatg ggagtgggac agaaatgata ttttcgcaaa
     4381 atttatttcg tcgtcccacc ccaacttgca cattattgta ggctgacttt ccgccagctt
     4441 ctatgttggg gccccgccaa cttgcacatt attgtaggct gactttccgc cagcttcttt
     4501 gttggggccc cgccaacttg cattgtctgt agaaatcggg aatccaattt ctctttgttg
     4561 gggccccgcc aacttgcatt gtctgtagaa attgggaatc caatttctct ttgttggggc
     4621 cccgccaaga tattacttga ataattcttt tagaattcta aataatgatc cgattaattg
     4681 aaagaagtct gctgtcattg tatatacctc cctttacttt ataaattatg cttgcttagt
     4741 atcagtcacc ttttcagttt tcactaaatc gtctgctaaa tgatgccaaa aatcttgtaa
     4801 ttcttctctt gtgcgcactg tatcagaact gtcttgtcct acaaagtcaa catgatccca
     4861 atcatgtttt gtaggcgtca cttgccaaat gcctttttga attttatccg tcgcatttgt
     4921 ataagcttga ttaaatggat gttgagaaga aataacggat actaaaccat cgttttctcg
     4981 ccattctttt tcagttgctt taccgattaa gttaccagta atcacaaatg ggaaaaacat
     5041 atttaagtct gctttttgtc tatcgctatt taatgctttg tgcgttgctt caccagtgta
     5101 tgttttatac acaatgttag ggttcaacga cgttttacga tttaaatctg ttgcaccctc
     5161 acgcgtcaga tcgtaaaatc cattatcttt tgatttccat aaattagatt gtttaacgcg
     5221 tttgacataa tcaatatatg attcatttgg cttctgtttt agaccccatt gagccaaccc
     5281 gaagtctact cttgaatttt tattaccaaa cattttaccg atatcaaata cgatttgtct
     5341 cactaaagct tcattaccag ctaaatctga tgcgtgtgta ccattatgtg gtgttcctaa
     5401 agtagtaatt gatgaaatca tattgtcatg attacctttg aatagtggag aaatttcgcc
     5461 accatgtttc ttttgatact ctatttcttc acgattacca ttacgcagta attcttctag
     5521 ttgacgtatc gtttgaccgc ccatactatg tccaactagg tgtaccttct gtcctggttt
     5581 ccagtctttg taaattcctt cgtatgtttt tccataacgt tcatgtccat attttgctgc
     5641 atgtgctgca ccataatcta cacgaccgcc tttgatataa taataaagtt caactgcgcg
     5701 gtcatagtta cttccaaaag cacttatact tgcttcataa gctttgtaac cattttcttc
     5761 taaatcttgg cgaatgttca ttttattacc gccccaataa tgagctaaca ctgaaggatt
     5821 aatatcatct gtaaacccat tgaaaccatg cactaaaacg ataggatcct gatttttata
     5881 ctgcccttgt ttggcaactt tattcgtttg atcatctttc gcttttgcag taagttcctt
     5941 tgtccctttg tttggtaacg ttgctactgc actacttttt aataaattta atccattttt
     6001 cttgtcattt tcctttgttg gttctactgg tgatttttga ttgtttttta aatctgaaac
     6061 tgatagtgtt ttttcgtctt tttgaggttt cacttcttgt tcatttttaa aattgtagtt
     6121 agtaatttta tcattcgctt gattattttg ttgtggttgt actttctcta tttctgtctc
     6181 attaacaatt tctcgttgac ttgtttgatc tgacttattt aatgatggtg cttcctttgt
     6241 taaactttgt gaaggtagat tgttattagc gtttgaatct tgactattat ctgcctcttt
     6301 tgactgttgc tgtgacgtca ctttactttg tttgccatca ttaatagcct gttctaaccc
     6361 ttgatttaca tcgctaggat tagcattgtt attttttgtt gtatcacgtt gaatattttt
     6421 attattaagc gatgattttt tatcatcctt tagttgatct ttattcaaat catctgtttt
     6481 acgatgattt tcttctgata ttgttttatc attatgcaag ttcttagtta aattttcagt
     6541 atgttgctgt tcgtcaacac cgttttcacg ttgataatta gcattttccc tagtttgttg
     6601 atccccaata gattgtgctg taactgtatc ctcctgtgaa tttcccatat tcacttgctt
     6661 ctcagctgct tgtgcttgtc caccacttaa aaatagtaat gtagctatta aaatggaaga
     6721 tgcacctaca ctaaatttac gaatactata cttattttga cttttcatca ttgtcagcac
     6781 ctctattttt ttattttaga taaacaaatt gataattatt cgaggaataa actcaaataa
     6841 tttacttaac caagcactca taatgaccat cctttctatt gtttaatttg acgagaaagc
     6901 gtttacattt gtatttaaaa aatatattct ccaaa
//
LOCUS       DAHHYJ010000132         6808 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000132 DAHHYJ010000000
VERSION     DAHHYJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.132"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            161..1354
                     /gene="fmtA"
                     /locus_tag="NBK22_002174"
     CDS             161..1354
                     /gene="fmtA"
                     /locus_tag="NBK22_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCD2415350.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETK
                     LSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRP
                     NILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFY
                     AKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(1891..2169)
                     /gene="qoxD"
                     /locus_tag="NBK22_002175"
     CDS             complement(1891..2169)
                     /gene="qoxD"
                     /locus_tag="NBK22_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCD2415351.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFVQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(2178..2783)
                     /gene="qoxC"
                     /locus_tag="NBK22_002176"
     CDS             complement(2178..2783)
                     /gene="qoxC"
                     /locus_tag="NBK22_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCD2415352.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(2773..4761)
                     /gene="qoxB"
                     /locus_tag="NBK22_002177"
     CDS             complement(2773..4761)
                     /gene="qoxB"
                     /locus_tag="NBK22_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCD2415353.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(4761..5861)
                     /gene="qoxA"
                     /locus_tag="NBK22_002178"
     CDS             complement(4761..5861)
                     /gene="qoxA"
                     /locus_tag="NBK22_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCD2415354.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
     gene            6163..6285
                     /locus_tag="NBK22_002179"
     CDS             6163..6285
                     /locus_tag="NBK22_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415355.1"
                     /translation="MIERKRKHFNKEFAKSNLSLNNEKWKFVCSKSKHFFKKSS"
     gene            complement(6433..6750)
                     /locus_tag="NBK22_002180"
     CDS             complement(6433..6750)
                     /locus_tag="NBK22_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCD2415356.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
BASE COUNT         2425 a         1060 c         1162 g         2161 t
ORIGIN      
        1 tttgttgtat agataacata ttagaaaaac ttattactat gtagcgtaaa gcataaatga
       61 atgataagtt aatttttgta agttgtatta ttaggcatct aggttcaaat ttttaacgat
      121 ttaaatatat aaaatgaata cattgtgagg caattatcaa atgaaattta ataaagtaaa
      181 actagttata catgcgtgtg tactattatt tatcattatt tctatagcgt taatttttca
      241 tcgattacag acgaagacac attctataga cccaatacat aaggaaacaa aattatcaga
      301 caatgaaaaa tatttagtgg atcgtaataa ggaaaaggtt gcgccgtcta aactaaaaga
      361 ggtatataat agcaaggatc ctaaatataa gaaaattgac aagtatttac aaagttcatt
      421 atttaacggt tcagtagcta tatatgaaaa tggcaaattg aaaatgagta aaggttatgg
      481 atatcaagat tttgaaaaag gtattaaaaa cacaccgaat acgatgtttt taataggttc
      541 agctcaaaaa ttttcaacag ggttactgtt aaaacagtta gaagaagaac ataaaataaa
      601 tatcaatgat ccagtaagta aataccttcc atggtttaaa acatctaagc ctatcccatt
      661 gaaagattta atgttgcatc aaagtggatt atataaatat aaatcctcaa aagattataa
      721 aaatttagat caagcagtta aagcgattca aaaacgtggt attgatccta agaaatacaa
      781 aaagcatatg tataacgatg ggaattattt agtacttgcg aaagtaattg aagaagttac
      841 aggtaaatct tatgctgaga attattatac aaaaatagga gatcctttaa aacttcagca
      901 cacagcattt tatgatgaac aaccttttaa aaaatatcta gcaaaaggtt atgcttataa
      961 tagtacagga ctttcattct taagacctaa tattttggac caatactatg gtgcaggtaa
     1021 tttatatatg acaccaacag atatgggtaa attaattact caaatacaac aatataaatt
     1081 attcagtcct aaaataacca atccattatt acatgagttt ggtacgaaaa agtatccaga
     1141 tgaatatcga tatggtttct atgctaagcc aacattgaat agacttaacg ggggattctt
     1201 tggacaagtc tttactgttt actataatga taagtatgta gttgtacttg cattaaatgt
     1261 aaaaggaaac aatgaagttc gaatcaaaca tatttataat gatattttaa aacaaaataa
     1321 accttacaat acgaagggtg ttattgttca ataattaata tagaagatat aacatgtata
     1381 tggcattaag gcatcgacct tatctgacca gtatacgagt tatatcttct tttttatagt
     1441 ggtaaaaagt ttaaagtata aggttgaaga aggatgagtt taaaaatatg tgttaactga
     1501 taaaagggga aatcatttgg tgagttggca tcagactaaa atgaatgaag acgaatacgt
     1561 tggtccatgc gtggtgtgaa tgttttctaa atctaactgg aaatgataat ttaataatgt
     1621 acacttttga ttgtttaaac atgtacaatt tttaaaaacc tagggataat tttattgacg
     1681 tgaacaacat tgagattatc tttagacata aatatggatt ttgaagttac tatatttatt
     1741 agttgtggtt gttaatgtat taattgaatt gattataatg attgtcttat aagatataga
     1801 aggggactta accatttaaa aattgaagga aaataaaaac cagctaacaa aagtgttagc
     1861 tggttaaact ttacattatt aaaatgttgt ttataagtgt gaagagtgac cgccttgcat
     1921 aacccaatat gttccgacaa cgaaacaaag tgtaattaca agagcaaaga taactttgaa
     1981 tgtttgtaaa cgtccatctt taccttcagt taaatgcatg aacattaata attgaagtcc
     2041 tgcttggacg aatgcaaagc caaagataat tgtcaacttc gcgtggaatg ttaatgacgt
     2101 gtatagtgtt acgtatactg ctaaaagcgt taatacgata gatgcgataa atcctacagt
     2161 atgtttcatt attgtactca tccgctatac accatcccta tcatatatac ggcagtaaag
     2221 atgaaaaccc aaacaacatc taagaagtgc cagtataaac ttactataaa taattttggc
     2281 gcattatatt tgtctaatcc gcgtcgttgg atttggatta ataaacaaat ggcccaaacg
     2341 atacctagcg atacgtgaca accatgcgtt cctaatagga taaagaaact agaccagtaa
     2401 gaaccaattg ttgggttaac gccttctgat gcatagtgtg cgaattcata aatttcgaat
     2461 ccaacaaaga ctaaacctaa aagtaacgta atgatcatcc aaaacatcat taacttttgt
     2521 ttttcttggc gcatgtagta aatagcaata ccacatgtgt aagaactgaa taataatgca
     2581 aacgtcatta ttaaaacaag aggcaattca aataactcag tagtcatttt acctgcataa
     2641 tcgccaccat gttgcaaagt taatagtgtc gcaaataggg taccgaataa cgcaaattcg
     2701 gctgtaatga aaatccaaaa gccaagctta tttaattcgc cttcatgtgt gcgtgaatca
     2761 atagtgtttg tatcatgact catgacttac agcctccctt tctttaatac gcgcttctct
     2821 taatctagct tcagtttcag caacttcagc agcagggata tgatatccgt gatcgatttg
     2881 gaaactgcga taaatcatag taccaaaaat accgaataaa caaattaatg ctggaattac
     2941 agtttcgaaa attaagaaga aaccgccgat agtcataaag ataccaatcc agaatccaac
     3001 aggagtattg tttggcatat gaatgtcttt gtaattatgg ttgtctaagt aatgacgacc
     3061 atgttctttc atatcaacaa atgtgtcgta gtcattccaa tctggtgtaa tggcaaagtt
     3121 gtatttaggt ggaattgctg atgcagtagt ccactctaga gtacgaccaa ggccatccca
     3181 gttatctcca gttgcttcac gtggagattt gaagtgactg tatacgatac taacaacaag
     3241 gaataagaat ccgattgcca ttaataatgc accgatagtt gaaattaagt ttaataagaa
     3301 ccaaccatct gatggcatat aagtgtataa acgacgtggc ataccatcta atccaagaat
     3361 gaattgtggt aagaaacaaa cgttaaatcc gatcatgaag aaccagaagc accatttgtt
     3421 taatgtttcg tttaatttgt aacccatcat ctttggatac cagaagatta aaccagctaa
     3481 gcaggcaaat acaacaccag taaccaatgt atagtggaag tgagctacta agaagtacgt
     3541 attgtgatat tgatagtcag ctgatgccat tgctaacatt acacccgtaa caccacctaa
     3601 aaggaagtta gggataaatg ctaatgagaa tagcattggt gactcaaatg taatacgtcc
     3661 tttatataat gttaataacc agttaaacaa tttcacacca gttggaatac caatcagcat
     3721 tgttgaaatt gagaagaatg agttgattaa cgcaccatta cccattgtga agaaatggtg
     3781 aacccaaact aagaaactaa ggaacgcgat accggcagtt gcccatacca tactttgatg
     3841 tccgaataaa cgcttacgag cgaatgtcgg gataatttct gagtaaatac caaatgctgg
     3901 aaggataacg atataaactt cagggtgccc ccatacccag aagaagttag cccaaagcat
     3961 tggcataccg ccatgtgcaa ctgtgaagaa tgctgtgtca aatattctat cagttgtcat
     4021 taatgctaac gctactgtta aaggagggaa agcaagaata acaattaatg tagtaataaa
     4081 tgttgttact gtaaacattg gcatttgcat aaacttcata gttggtgttt tacatcttaa
     4141 aattgttaca aagaagttga tacctgtagc taaggtacca agccctgaaa tttgtatagc
     4201 tattaagtaa tagttaacac ccggaccagg actgaattca cctgctagtg gcgcatagtt
     4261 tgtccaacca gctgctggtg aaccaccaat aataaatgac aggttgaata aaatcatacc
     4321 tgcaaagaat agccagaaac ttacgttgtt taatactggg aatgcaacat cacgtgctcc
     4381 aatttgtaat ggaacaacga tattccataa accaaagata aatggcattg ccatgaagat
     4441 aatcatgatt acaccatgtg tactaaaaat ttcgttatag tggttagatt ctaaaaattt
     4501 gttatcaggt actgttaatt gcgcacgaat aagtaacgca tcaataccac cacggacgaa
     4561 cattaatacg gcacagatta aatacataat accgattttc ttatggtcta cagatgtgaa
     4621 ccattctttg taaagatatt tccataattt aaagtaagta attactgcga ttaaaccaat
     4681 aactaagaat ggggcaccaa tttgtgccat tgtaatcatc cagttacctt taactagtaa
     4741 ttgatcccat ggaaaattca ttaatgtcca cctccatgat catcattgtc ttgatcttgc
     4801 gcatcttttg aaattttctt catttctttc gcatttttcg attcatcttt cttgaactca
     4861 ttgttatatg gttcgtcatt tccaagaatc atcaacttca taccatgtcg tttatagttc
     4921 gcatttgtaa tttgagcttt acgagcaggt attaatggtt tgtctgatac atctttaaac
     4981 atattttctt cactagtgaa gtttggatct ttcaattcga aattgaaacg tttatatgca
     5041 tagaagatgt attctggatc ggctgctgga tcaacaaacg ccatatgtgt accattaaat
     5101 tctaaagctt tattaggtgt gcttggtaat aattgtttat caaatgtatc ttgatctaac
     5161 gttttcttac ctttaacttc tttcacccat ttgtcgtagt ctttttgact aacggcattt
     5221 actttaaatg tttgacgtga gaatccttca ccattgaagt tagagttacg acctctgaac
     5281 gtaccagttt gagatgcttc taacgtccaa ttcattgtca tgccagtcat ggcatatttt
     5341 tgaccaccta attgtggaat ccagaaactt gtcattgtat ccatagcttg aagcttaaat
     5401 acaacaggac gatctttagg gattgttaat gtgttaacag tctctatatg ttcatctgga
     5461 taagcaaaga accatttgta tcctgcactt actgcatata caaccattgg atctttctca
     5521 ctcttcggtg gtttttcgta atcgtataaa gttttaactg taggaatagc taaagcagca
     5581 acgattatga taggtattac aaaccatatt gtttcaatga tggcattatg gtgcatctta
     5641 ccagattcgg cattcttatt ataactatac ttgtaaataa aaatggcgaa catgccaagt
     5701 acaacgaaac aaataacaag catgaagacg attgaataaa gaatcaagaa cttctgacta
     5761 cttgctactg gcccttttgc gttgaaaatt tctatatttg aacaaccact aagtaaaatt
     5821 agtgtgccaa ataatagaag caaagactta aattttgaca cttttttgac ctcctaatac
     5881 tacaaatgta gggcttaaca ttaattttaa gttattacac aatatttaca agggcttatg
     5941 ggaaaaaaat taataaaatt gtatcaaaaa tgttgataaa tcaaggtgtg acgtgggttc
     6001 acacatttgt taaaattatg tgtacatttt gtgactaata gcgtttttga aaaaattgct
     6061 tcatatttaa aaggaaagtt gagaatagct ttcaattaaa ggttaattga atgaaaataa
     6121 aaaaagtttg aaattgtcta ataaattgaa aatgatggct agttgattga aaggaagcgc
     6181 aaacatttta ataaggaatt tgccaaaagt aatttatcgt taaacaatga aaagtggaaa
     6241 tttgtttgtt caaaatcaaa acattttttt aaaaagagca gttaagtttt aataatttga
     6301 taggtagtcc tttggtatac agaaaaaata caatatttgg gttaaagttg cagatgaagt
     6361 tagaataaca aataaaatgg tagcaaaaca tgttcgtttg ctaccatttt atttgcagta
     6421 tagtgatgag ggctatttaa cctcaatata cctagtggaa attttaattg caccatctga
     6481 atcagtgaca tgatatttaa tttgatattt accagattta gatgtatcaa tttggccatc
     6541 gactttaatt ttatcggtta aatctccatc ttctttatca aatgcactta tgccgtttag
     6601 aaggttataa tcttgacctt tcttaataac gatatcatta gcgcctttaa tttgtggtgt
     6661 agtattcgtc gttgcatctg catttaaatt tggtgttact agtgtagcag aaacaccgag
     6721 ggctgataat gactgtaata gtttattcat aatgtaacct ccgaatgata agtcatttct
     6781 gagttagctt tactatacct taatttat
//
LOCUS       DAHHYJ010000133         6756 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000133 DAHHYJ010000000
VERSION     DAHHYJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.133"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(164..1870)
                     /gene="acsA"
                     /locus_tag="NBK22_002181"
     CDS             complement(164..1870)
                     /gene="acsA"
                     /locus_tag="NBK22_002181"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCD2415357.1"
                     /translation="MKVEVYKGAQGKHNLKDYEEIYNTFDWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGLGDKIALNYKDEYRKESYTYKDMQRLSNKAANVLSEHAEVAKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVADRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            2039..2671
                     /locus_tag="NBK22_002182"
     CDS             2039..2671
                     /locus_tag="NBK22_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD2415358.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTYEYLKALTFDAHLTAFRD
                     AEDQYEALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLHLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            2696..3865
                     /locus_tag="NBK22_002183"
     CDS             2696..3865
                     /locus_tag="NBK22_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCD2415359.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVVKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(3963..4952)
                     /gene="ccpA"
                     /locus_tag="NBK22_002184"
     CDS             complement(3963..4952)
                     /gene="ccpA"
                     /locus_tag="NBK22_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCD2415360.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(5025..5138)
                     /locus_tag="NBK22_002185"
     CDS             complement(5025..5138)
                     /locus_tag="NBK22_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415361.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
     gene            complement(5492..6583)
                     /locus_tag="NBK22_002186"
     CDS             complement(5492..6583)
                     /locus_tag="NBK22_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCD2415362.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLSDLYNAKKLK"
BASE COUNT         2158 a         1264 c          959 g         2375 t
ORIGIN      
        1 attgttatgt catcattttc aaatgctttc tacgaaatga tatgacacaa gttaacagct
       61 ctttatattg cttatcataa agtaaactaa tatttaaata ttaacatgaa atatgttgtc
      121 tccctttatt cttcgaaaga aatcttcaat aacattcatg tcattattcc attgtactta
      181 aatccccagc atctaaattt aattcccaag cttttaatac acgtctcata attttacctg
      241 accgtgtttt aggtaattta tctttaaatt cgatttcacg tggtgctgca tgtgccgaca
      301 aaccttcttt aacaaataaa cgaatttctt cttttaattc gtctgttggt tcatatcctt
      361 ttctcaatgc aacaaacgcc ttaattattt caccgcgaac cggatcaggt ttaccaataa
      421 ttcccgcttc ggcaactgct tcgtgttcaa ccaatttaga ctcaacctca aatggtccaa
      481 ctcgttcacc agctgtcata attacatcat caacacgtcc ttggaaccag aagtaaccat
      541 cttcatcttt atatgccgaa tcaccagata cataccagtc tccaataaaa tatgatttat
      601 atttttctgg attcttccag atacgataca tcattgatgg ccagcctttt tttatagcaa
      661 ggttgcccat tcgatttggt ggtaattcat tacctgcatc atcgataatt gcagcttgaa
      721 taccaggtaa tggtttgccc attgagccaa gcttgacgtc catcgttgga tagttaacaa
      781 tcatatgtcc acctgtttct gtcatccacc aagtatctaa caccgttaaa ccgtatactt
      841 ttttcgccca ttttataact tcaggattta aaggctcacc tactgataga atcgaacgta
      901 acgatgacaa gtcatatttc tcaacaatat cgtcaccagc actcattaac attcttaaag
      961 ctgttggtgc cgtataccaa atcgtcactt taaaatcttc aatcatacta taccactgtt
     1021 ctggtgaaaa gcgaccacca gctatacaat ttgtagcgcc atttaaccat ggtgcaaaaa
     1081 taccataaga tgttcctgta acccaacctg gatctgctgt acaccaataa acatcatctt
     1141 cttgtaaatc taatacatat tttccagaaa tatagtgcac taacattgct tgttgaacat
     1201 gcaatacacc tttaggttgc ccagtagaac ctgatgtata atgtaaaatc aaaccatcat
     1261 ccgactttaa ccattcaatg tcaaattcat cgctagcagt ttccatcaaa ctaatgaagt
     1321 ctatgtaatt gtcttctaca tcctcatcta cgacaacaat ttttttcaag ttcggtaatt
     1381 tatctacagg tactcgaggt aacaatgcct tattagtaat taacacttta gcttcactgt
     1441 tctctaatct atccgcaact gccttttcca taaatgcttc aaataacggc ccaacaattg
     1501 caccaatttt taaaacgcct aacaacgcaa aatatagttc aggtgtacgc gacataaata
     1561 taaatactct gtcacctttg gcaacttctg catgttcaga caaaacattc gctgctttat
     1621 tagataaccg ttgcatatct ttataagtat acgattcttt tctgtactca tctttgtaat
     1681 ttaacgctat tttatcccct aatccttgat ctacatggcg atctatacat tcatatgcca
     1741 tgttcatttt tccagtttca ctccaagaaa atgcttgttc tacgtctttc caatcaaaag
     1801 tattatatat ttcttcataa tctttaaggt tatgtttacc ttgcgctcct ttataaactt
     1861 cgactttcat ttgaaactcc ccctttgtta tgtgaaaacg ctttctttct caatattata
     1921 cataacattt ctaattattc aaaattttta cttttatcct ttttctagta ttaattattg
     1981 taaattttct atataatgaa tttaatacaa tcacgacgtc gattgaggag ttgaaaatat
     2041 gaatcattta aagacgtatc aatccgaaga ttattacatt catgacaagc aatttgttat
     2101 tgaaggtcct ttaacatacg aatatttgaa agcgcttact ttcgatgcgc atttaaccgc
     2161 atttagagat gctgaagatc agtatgaagc tttgttagaa attacaacat taccagaagg
     2221 tagaatttat gttgctcgcc aagatcaact cattgtgggt tatgtcactt tccactatcc
     2281 tgatgaaatt gagcgctggt ctacaggtaa ccttccatat ttaatcgaat tgggggcaat
     2341 tgaagtcagc atcaatttta ggcaattaca tcttgcagaa aagctaatac aacttagcct
     2401 ttctacacca gaattcgagg attatatcgt tataactact gaatattact ggcattggga
     2461 tttaaaaaat tcaaagttag atgtatttga ctataaaaaa ttaatgcagc ggttaatggc
     2521 aactggtgga cttgaaatat tcgctacaga tgatccagaa ataacaagtc atccagctaa
     2581 ttgtttaatg gcaagaattg gcaaaaatat tacattagaa cagcaacaag cgtttgatga
     2641 tattcgttat atgaatcggt ttttctttta atttttggaa tatcggaggg attatatgca
     2701 acaacattca tcaaaaactg catatgttta ttcagataag ttattacaat atcgatttca
     2761 tgaccaacat cccttcaatc aaatgcgttt aaaattaaca acagagctac ttttgaatgc
     2821 aaatttattg tctccagaac aaatagtaca acctagaatt gcaacagatg acgaattaat
     2881 gttaattcat aaatatgatt acgtcgaagc aattaagcat gcttcacatg gcattatcag
     2941 tgaagatgag gctaagaaat atggattaaa tgatgaagag aatggtcaat ttaagcatat
     3001 gcaccgccat agtgccacaa ttgttggagg cgctttaact ttagcagatc ttattatgtc
     3061 aggcaaagta ttaaatggtt gtcacttagg tggtggattg catcacgctc aacctggtcg
     3121 agctagtggt ttttgtatat acaatgatat tgcaattacc gcacaatact tagctaaaga
     3181 atacaatcaa cgcgttttaa tcatagatac cgatgcacat catggagatg gtacacaatg
     3241 gagtttctat gccgataacc atgttactac ttattctatc catgaaaccg gaaaatttct
     3301 tttcccaggc tctggtcact atactgagcg cggtgaagat atcggctatg gacacactgt
     3361 aaatgtccca cttgaaccgt atacagaaga tgcatcattt ttggagtgtt ttaaattaac
     3421 agttgagcct gtcgtaaaga gttttaaacc tgatattatt ctaagcgtaa atggtgtcga
     3481 tatacattat cgtgatccac taactcatct aaattgtacg ttacattcat tatatgaaat
     3541 tccatatttt gtaaaatatt tagctgattc ttatacgaat ggaaaggtaa ttatgtttgg
     3601 tggcggaggc tacaatattt ggagagtcgt accacgtgca tggagtcatg tattcttaag
     3661 tttaattgat caaccaattc aaagtggtta tttaccgtta gaatggatta ataaatggaa
     3721 acattattca tctgaattat tacctaaaag atgggaagat cgtttaaatg attataccta
     3781 tgtccctcgc acaaaagaaa ttagtgaaaa aaataaaaaa ttagctttac atatagcgag
     3841 ttggtacgaa tctactcgtc aataagtaac ataatctgca acgattttaa aagataaata
     3901 aaaggcattc ttccaacacc catataattc gggtcgttag atgaatgcct aattttgtga
     3961 atttattttg tagttcctcg gtattcaatt ctgtgaggta aaactacatt tggttcttct
     4021 atcttttcat cattcatata ttttgttaat aagcgcatcc ctactgcacc gatatcatat
     4081 aatggttgaa taacactaga aagttgtggt ctaaccatct caactaaacg tgtattattg
     4141 aaactaataa tttgtaattc ctctggaact ttaataccag catccattgc actatgcata
     4201 ataccaatag cttcttcgtc actaatacat aatatagcat ctggaagatt acctttcatt
     4261 ttggcaaaag cttttacgcc ttctttataa ctttcagcac cagaacaatt caatgtatca
     4321 cctaattgaa gaccattttt atttaacact tcagttaaac cttctaaaac atcttcttga
     4381 gcttttttag aatgttctcc acctactaaa gcaaatgatt tagcgccttt ttcaattaat
     4441 tctcccgtaa tttctttcgc agcttcagta aaatcaatat taactgatgc tatatgtgca
     4501 tccttaccat ttgttcctga tactactaca ggtacagatg attgatttat caattctttc
     4561 atttcttcag taattgtacc accaaggaaa ataataccat caacttgttt acttaataag
     4621 ttattaaaaa tttctttttc cttttcagga tcgttatctg aatttgaaat aattgagtga
     4681 tatttataca ttgttgcaat atcttcaagt ccacgagcaa gttgtgaata atagatatta
     4741 gatatatctg gaatgatcac acctactgtt gttgtctttt tactagctaa acctctagca
     4801 acagcatttg gacgataatt caaacgctta atgacttcgt taactttatt tttagtttct
     4861 gctttaacat tttggttccc attaacaaca cgcgacactg tggccataga gacacgcgct
     4921 tctcttgcta catcatatat agtaactgtc ataatttcct ccttgtaaac gttttattca
     4981 ttataataac atgttttcat ttcattttca tagtttacca tattttaatt tacagtaaca
     5041 tatttttgtt tggaaaaatt tagcgaaaaa cttgaaataa aaacaaacgc taaatgataa
     5101 cgaacgatat attttaattt tatataaact tttttcattt ttaattgtaa tattttgaga
     5161 aagccttttc aaatatcaat tgcatttgtt gaaaaatgtt cacatttcag caacaaatgt
     5221 gtgaatctat tatattttac tgataattca tagttataat tttaattact gctatcagtc
     5281 ataatcttta ataattgtga aattgaacaa gtgtcgaagt attataacaa tcgttaagtg
     5341 cagtatacct ttatttctat acaaaaaata atagccactc taaaagtaat atactcttag
     5401 aatggctact agtttaaaat tgtcgattca aagataatat tctacttcaa catgacatat
     5461 tagttaagta gtctatagtt tccttggaat attattttaa ctttttagcg ttatataaat
     5521 cagacaaagg ctttaattca tcataaaatg cttggaattc atctaaatcc atttgttgac
     5581 ccgcatcact aagcgcaaca gatggatctg gatgcacctc agccataact ccatcagcac
     5641 caactgctaa tgctgctttc gcagttggta acatgatatc tttacgacct gtactatgcg
     5701 taacatctac catgactggt aagtgtgtac cttgttttaa aattggtact gctgaaatat
     5761 ctaaagtgtt acgtgtcgcc ttttcataag ttcggattcc acgttcacat aaaataatat
     5821 tttgattacc ttgtgaagca atgtattcag ctgcataaac aaactcttcg attgtagcag
     5881 ataaaccacg ttttaataga ataggctttt tcgtacggcc agcttctttt aataactcga
     5941 agttttgcat attacgtgca ccaatttgga atacgtctaa atactcatca gccacttcaa
     6001 aatcatttgg atttacgatt tcgctgacaa catttaaatc atatttatct ttaatctgtt
     6061 taagtatttt aagtccttca acacctaggc cttggaaatc atatggtgat gtacgtggtt
     6121 taaatgcacc gccacgaata aatttttcac ctttagcatg taagttttta gcaacagctt
     6181 caacttgttc aaatgattca actgaacatg gcccaaatac aaatgattta ttgccgtctc
     6241 caataatgcc cccattatca aatgttacaa tcgtatcttc aggtttcaac ttacgtgata
     6301 catataaatg tttttcattt tcagattttt gtaaatctgt agaggctttg aaaatttctt
     6361 taaataattg cttaatagta ttatcgttga atggtccttt gttactatcg attaagtcgt
     6421 taagcatttc tttttcacgt tgtggatcat agatacgtgt accttgtttt aatttttctt
     6481 ccccaatttt ttgtgctagt tcaccacgtt tagataataa gtctaaaatt tgatgattca
     6541 gtgatacaat ctcacttctg tatgattcta atttattact catccttact cacctctact
     6601 aattatttca agtatattcc actttaaagc aataaaatat aatgttaaaa aatattctaa
     6661 caatatcata tgtatagttc aaccctaaat tgacagaaaa taatcaattt tctaattata
     6721 tgattcgaga ctgacatttc gattttctca gtcatt
//
LOCUS       DAHHYJ010000134         6628 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.134, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000134 DAHHYJ010000000
VERSION     DAHHYJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.134"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(270..896)
                     /locus_tag="NBK22_002187"
     CDS             complement(270..896)
                     /locus_tag="NBK22_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415363.1"
                     /translation="MTVKVISLSELLTGDKQEVKRKIPSVLNILNSFETISISGSESA
                     HDVDLFLKNKSIAFDRQNLSRTHLVFSQFKSKQILVGYFTISNKPLVFTKRMLDKISN
                     TLKKKLYQKGETHSGNDNLIIQRYLIAQIGKNYSEEALATKSINGNDLLSLAYEKVLE
                     GANIFGGSYIWIEYEDVYRLREFYRKFRFTEIKDHTSENNLKMAILKI"
     gene            complement(893..1072)
                     /locus_tag="NBK22_002188"
     CDS             complement(893..1072)
                     /locus_tag="NBK22_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415364.1"
                     /translation="MATISITTDYKITKKSAQKLIDAMEINENNSNVNKTNIKATKIK
                     STCEIENLLKDYRSN"
     gene            complement(1606..3222)
                     /gene="groL"
                     /locus_tag="NBK22_002189"
     CDS             complement(1606..3222)
                     /gene="groL"
                     /locus_tag="NBK22_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCD2415365.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDARVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEEGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(3298..3582)
                     /gene="groES"
                     /locus_tag="NBK22_002190"
     CDS             complement(3298..3582)
                     /gene="groES"
                     /locus_tag="NBK22_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCD2415366.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            3757..4500
                     /locus_tag="NBK22_002191"
     CDS             3757..4500
                     /locus_tag="NBK22_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD2415367.1"
                     /translation="MTRLWASLLTVIIYILSQFLPLLIVKKLPFVQYSGIELTKAVIY
                     IQLVLFLIAATTIILINLKIKNPTKLELEVKEPKKYIIPWALLGFALVMIYQMVVSIV
                     LTQIYGGQQVSPNTEKLIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGN
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRLAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(4525..5706)
                     /locus_tag="NBK22_002192"
     CDS             complement(4525..5706)
                     /locus_tag="NBK22_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415368.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATYTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHESNQTGNEGTGSNSRDANPDSNNVKPDSNNQNPST
                     DSKPDPNNQNPSPNPKPDPDNPKPKPDPKPDPDKPKPNPDPKPDPDKPKPNPNPKPDP
                     NKPNPNPSPDPDQHGDSNHSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNPTGND
                     FVSQRFLALANGAYKYNPYILNQINKLGKDYGEVTDEDIYNIIRKQNFSGNAYLNGLQ
                     QQSNYFRFQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDKPGSKQRSFEPH
                     EKDDFTVVKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNESQR
                     R"
     gene            5903..6529
                     /locus_tag="NBK22_002193"
     CDS             5903..6529
                     /locus_tag="NBK22_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCD2415369.1"
                     /translation="MGLFKKDKKAMSFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
BASE COUNT         2037 a         1171 c          967 g         2453 t
ORIGIN      
        1 agagataatt ttattgctgt gaacaacaat gaaattatct tcagacataa atacggattt
       61 tgaagttact actctttcat atttacaaaa attaataatt gttatgatga acttaaacat
      121 gaaaataagc aaaagtgaaa tagctagaca catgggtgtt gatagaagag cagttgataa
      181 atattcaaat gtctttgact tttattttat aaattcttcc ataaaaataa ccacactcct
      241 aaatcaatag gtgggtgtgg ttttgtgcct tatattttaa gaatagccat cttcaaatta
      301 ttttcacttg tatgatcttt gatttctgta aatctaaatt ttctataaaa ttctcttaat
      361 ctataaacat cttcatactc tatccaaata tatgaaccac caaatatgtt cgcaccttct
      421 aaaactttct catatgctaa agataacaaa tcgtttccat ttatggattt tgtagccaat
      481 gcttcttcag aataattttt tcctatttga gcaattaagt atctttgtat gattaaattg
      541 tcatttccac tgtgagtttc acccttttga tataacttct tctttaacgt gtttgatatt
      601 ttatctaaca tacgttttgt aaaaactaag ggtttattgc taattgtaaa atagccaact
      661 agtatttgtt tgcttttgaa ttgtgaaaaa actaaatgag ttctagacag gttttgtcta
      721 tcaaatgcta tagacttatt tttcaaaaat aaatcaacat cgtgcgctga ttcacttcct
      781 gaaattgata ttgtctcaaa tgaatttagt atatttaaaa ctgaaggtat ctttcgctta
      841 acctcttgct tatcacctgt taacaattct gaaagtgata ttacttttac agtcaattac
      901 ttcgatagtc cttcaataga ttttcaatct cacaagttga ttttatttta gtcgctttta
      961 tgtttgtttt gttcacatta ctattatttt cgttaatctc cattgcatct attagttttt
     1021 gcgctgattt ttttgtgatt ttataatctg tagtaatact tattgtagcc atactactca
     1081 cttccttatt ttacttcatt tgacaactaa gtatacattt tatacgttgt cttcttgttt
     1141 acaatgtatc atgaaagcat cttttcgaca atagattcaa taaatttttt agtgtatatg
     1201 attttacact taaaccaatt tgtttcttag cataaaaaca cccatactgt aactagtgtg
     1261 ggtgttttca aaatgcagac agcttcttac tacaatacgc aagtgagtcc tctgcaaagc
     1321 ctaattgatt accggaaatc taattacatt acaacatact agaagaaact ccttatatac
     1381 tcaaaaggta tactaaactt ctctatataa ttttaacatt gaaaaagccc cccattatga
     1441 atctcaataa cacatcagca cataataaag acgcctctca taagtttagt caactaatgg
     1501 aaggcgtctt aaattaacat attttcatac gctctaatat ttatttggaa cgaatcatac
     1561 tcgtctatca tggaatgttc tagttagtca aaactataaa cagttttaca tcattcctgg
     1621 catgccaccc atgttaggtt ggtcattatt tttttctgga attgatgcta caaccgcttc
     1681 agtcgttaag aacattgctg caacacttgc agcatgttgt aatgctgagc gtgttacttt
     1741 agttggatca acgatacctt cttctaacat attaacccac tcgtttgtag cagcgttaaa
     1801 accaacaccc ggctctgcgt ttttcaaacg ttctacaata acagaacctt ctaatcctgc
     1861 attttcagca atttgacgaa ctggtgcagt taatgcttta agtacaatat ttacacctgt
     1921 ttcaatgtca ccttcagctt caatttcact tactttttgg taaacattta ctaatgcagt
     1981 accaccacct gcaacaatac cttcttcaac tgctgcacgt gtagaattta atgcatcttc
     2041 aatacgtaat ttacgttctt taagctctgt ttcacttgct gcacctactt tgataactgc
     2101 aacaccacct gctaatttag ctaagcgctc ttgtaatttt tcacgatcaa agtcagattc
     2161 agtttcttca atttgagatt tcaattggct aacacgtgca tcaatgctgt tttcgtcacc
     2221 gtcaccatca acaacagtgg tattatcttt agttacttct actttacttg cagtacctaa
     2281 catatcaatt gatgcatctt ttaaatctaa gcctaaatca tcagtaatca cttgcgcacc
     2341 agttaaaata gctaaatctt caagcatcgc ttttctacga tcaccaaaac caggtgcttt
     2401 tactgcaaca gctgtaaatg tgccacgcat acggtttagc acgatatttg ttaatgcatc
     2461 gccttcaact tcatcagcta caattaagat tggacgatta gattgaacca cttgttctaa
     2521 taaaggtaag atatcttgga aagacgagat tttcttatct gttactaaaa tgtatgggcg
     2581 ttctaattca gcaaccattt tatctgaatc agtaaccata tacggtgatt gataaccacg
     2641 atcaaattgc ataccttcaa ccacttctag ttcagtgttt agtccatttg attcttcaat
     2701 tgtaatgaca ccatcgttac ctactttttc catagcttca gaaatataac gtccaatttc
     2761 ttcatctgct gctgaaatcg cacctacttg cgcaatttca tttttatttt caactttttg
     2821 agaattttca tgtaacgctt caacagcaac tttaactgct ttgtcgatac cttgtcgtaa
     2881 accaactggg ttcgcaccac ttgtaacatt tttcaatcct tcttgaatca ttgcttgagc
     2941 taatactgtt gcagttgtag taccgtcacc tgcaatttca tttgtcttat ttgcgacttc
     3001 ttgaactagt ttagccccca tattttcata tggatcttct aattcgattt ctttagcaat
     3061 cgttacacca tcgttcgtaa ttaaaggtgc tgtaaactct ttatctaata caacattacg
     3121 tcctttagga ccaatcgtta ctttaactgc atttgctaat tggtctacac cacgtaacat
     3181 tgcttgacgt gcatcttcag agaatttcaa ttgtttaacc ataaatgata aacctccatt
     3241 tgtttagtca tatttcattt tcgttcttta caatttatct atgaattaag ttctgtatta
     3301 ttcaataact gctaaaatat cttcttcatt taataccaga tatgtttcat tatctcgttt
     3361 aacttctgta ccagcatatt gttggaacac gacacggtcc ccttctttca cttcaggagt
     3421 cactcttgta ccatcattta atagacgtcc agttcctact gcaacgataa cgccttcgtt
     3481 tgatttttct ttagcactat cagttaaaac aataccactt ttagttgttt gttcttgttc
     3541 ttttttctca ataatcacac gatttccaat tggttttagc atgattgttc ctccttaaaa
     3601 aacctaaagt ttagcactta acattaaaga gtgctaacat acatttataa taatcaaatt
     3661 tggtcaaaat ttcaagtcat aaccttttaa ttaagttttg taacttcaag ttatttacga
     3721 taaaataaat tataaacaaa tatttggagg aaaattatga caagattatg ggcatcattg
     3781 ctaactgtta ttatttatat attgtctcaa tttttaccgc ttctcattgt aaaaaaatta
     3841 ccatttgtac aatatagtgg catagaactg actaaagcag tcatttacat acaacttgtt
     3901 ctatttttaa tcgccgccac gacgattatt ttaattaatt taaaaattaa aaatccaaca
     3961 aaattagaat tagaagttaa agaacctaaa aaatatatca ttccatgggc attgcttgga
     4021 tttgcattgg taatgattta tcaaatggta gtgagcattg tattaacgca aatttatggt
     4081 ggacaacaag taagtcctaa tacagaaaag ctaattatta ttgctcgaaa aatacctata
     4141 tttatcttct ttgtatctat tattggtcct ttattagaag aatatgtatt cagaaaagta
     4201 atctttggag aattatttaa tgcgattaaa ggtaatcgta tcgtggcatt tattattgct
     4261 acaacagtaa gttcattaat atttgcatta gcacataatg atttcaaatt tattccagtt
     4321 tattttggca tgggtgtcat tttttcatta gcatatgttt ggacaaaacg gcttgctgtt
     4381 ccaattatta tccatatgtt acaaaacgga tttgtcgtta tattccaatt acttaatcct
     4441 gaggctttga aaaaagccac ggaacaagcg aattttatat atcacatttt tattccataa
     4501 taaaaaaaac gaatcacaca cggattatcg tcgctgtgat tcgtttttat ttttcttttt
     4561 atttcgcttt acaaataaga atagcatgat tgaaaatacc accatcatag aacatacaat
     4621 tgctagccaa cttttactat atgcagttga cgcacttttc ttattatctt cttgtttttt
     4681 aactactgta aaatcgtctt tttcatgcgg ttcaaatgag cgttgttttg aacccggctt
     4741 atcttcgggc tttttcaaat ctggcattga tccaatttca ccagtaatta atgcgagtac
     4801 ttgttcatct aaattacgat agtacctttc tgatttcaat ggattgaaat attggaatct
     4861 aaagtaattc gattgctgtt gtaatccatt taaatatgca tttccgctga aattttgttt
     4921 tcgaataata ttataaatgt cttcatcagt aacttctcca taatctttgc ccaacttatt
     4981 aatttgattt aaaatatacg gattatactt gtaagcccca tttgccaagg ctaaaaatcg
     5041 ttgggataca aaatcattac cagtaggatt ttgtgagttt ccttgattcc catttggttg
     5101 ccattgacct tgattagatc catctgaagc atttgggttc catgtccccc catttttcga
     5161 gccaccagaa tgattggaat ccccatgttg atcgggatct ggtgacgggt taggatttgg
     5221 cttattaggg tctggttttg gattcggatt tggctttggt ttatctggat ctggttttgg
     5281 atccggattt ggctttggtt tatctgggtc tggttttgga tccggttttg gtttcgggtt
     5341 atctggatct ggtttaggat tcggacttgg gttttggtta tttgggtctg gttttgaatc
     5401 tgtacttggg ttttggttgt ttgagtctgg cttcacatta ttcgaatcag gattagcatc
     5461 acgactattc gaacctgttc cttcattacc cgtttgattt gattcgtgag gtgtcccagt
     5521 ctcattatta ttcatttcac tatttactga tggtgaagtt atattatgat tcaaattagt
     5581 agttggtttt tctccattta agttatcctt tgcttcaact gtgtacgttg caagccctaa
     5641 actactacta cttaaaatta aaatacttgt tgaaagtaac attttcttaa accaatgata
     5701 tgacatttca cgagacacct ccctttttca tgtaagtatt aatctaagta taataaatat
     5761 gcgttgaaaa tgcatttata tttggaattt gaaaatgaat attaatattc ttgaaatttt
     5821 cgtaaatatt gctttacaag tacgagtata aattttatac ttaatctatt aagtattctt
     5881 tactaaagaa ggagcaataa ttatgggctt atttaaaaaa gataaaaaag caatgtcttt
     5941 tgacaatgca atggaagaaa gacgttcaat atataatttg aaagattcga tatcaattag
     6001 cgatgacgaa ttagaatcaa tgattgcaca tgcagttaaa catgttcctt catcatttaa
     6061 ttctcaatca actagaattg ttctcttgtt aaatgacaac aataataaat tttgggataa
     6121 cacaaaagca attttaaaag aagtcatggg agagaatcgt gatttcgagc caactgaaca
     6181 aaaaattgat aacttcaaac attcatacgg tacaattttg ttttatgaag atcaagatgt
     6241 tgtaagtggt ttacaagaac aaatgccaaa ctactatgat aattttgcaa tatggtcaac
     6301 tcaaacaaat gccatgcatc aatttgcaat ttggactgca ttagctacta aaggcatagg
     6361 tgcttcatta caacactata atccacttgt tgatgagatg acttcaaatg aatttaatat
     6421 tcctaaaagt tggaaactta ttgctcaaat gccatttggt gatattcgtg aagctgctgg
     6481 cgaaaaaaca tttaatccag tggaagatcg tttcgtaata aaaaaataat caatatattg
     6541 ttaggaatgg gacagaaatg ataaagaatc attagttatt cattatgtag tagttcttac
     6601 acattagccg ctgctaatgt gcacttaa
//
LOCUS       DAHHYJ010000135         6615 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000135 DAHHYJ010000000
VERSION     DAHHYJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.135"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..131
                     /locus_tag="NBK22_002194"
     CDS             <1..131
                     /locus_tag="NBK22_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415370.1"
                     /translation="MKACYSYFFIFSYRIMSKKVYNMNVKTNFLKWECEVRRNVNK"
     gene            180..506
                     /locus_tag="NBK22_002195"
     CDS             180..506
                     /locus_tag="NBK22_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCD2415371.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            536..1555
                     /locus_tag="NBK22_002196"
     CDS             536..1555
                     /locus_tag="NBK22_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD2415372.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SKDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMTRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGEKAPSILKAGEAFNSKTSFRIEE
                     KVEND"
     gene            complement(1789..2442)
                     /locus_tag="NBK22_002197"
     CDS             complement(1789..2442)
                     /locus_tag="NBK22_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCD2415373.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPSLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGDALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDLLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            2616..3302
                     /locus_tag="NBK22_002198"
     CDS             2616..3302
                     /locus_tag="NBK22_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCD2415374.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKIVQYLGETFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(3368..4627)
                     /gene="sdpC"
                     /locus_tag="NBK22_002199"
     CDS             complement(3368..4627)
                     /gene="sdpC"
                     /locus_tag="NBK22_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCD2415375.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLIILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDEETNHTEAEKSSSNIKNAEKTGVATASTVGVAKNDTENTVADEQSIHEGTEKTEP
                     QHHIGNQTESNHDEDHDITSESVESAESVKQAPQSDDLTNDSNEDEIEQSLKEPATYK
                     EDRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            4917..5852
                     /locus_tag="NBK22_002200"
     CDS             4917..5852
                     /locus_tag="NBK22_002200"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCD2415376.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCEILVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(6108..6527)
                     /gene="fosB"
                     /locus_tag="NBK22_002201"
     CDS             complement(6108..6527)
                     /gene="fosB"
                     /locus_tag="NBK22_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCD2415377.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLEDRLNYYKEAKPHMTFYK"
BASE COUNT         2219 a          897 c         1233 g         2266 t
ORIGIN      
        1 tgatgaaagc gtgttactct tattttttta tatttagcta tagaataatg tctaaaaaag
       61 tgtacaatat gaatgtgaaa acgaactttt taaaatggga atgtgaagta agaagaaatg
      121 taaacaaatg atatgtagcc gatagattat tggtagcaaa tgtgtgatgg agtgatttca
      181 tgttatatcc gatttttata tttatattag ctggcttatg tgaaataggt gggggatacc
      241 tgatttggct gtggcttagg gaaggacagt cttcacttgt tgggctaata ggcggtgcga
      301 tactcatgtt atatggtgtc attgcgacat ttcaatcatt tccatcattc ggaagagtat
      361 atgcagcata tggtggcgta tttatcatca tgagtttgat ttttgcgatg gtcgtagata
      421 aacaaatgcc agataaatat gatgttatcg gtgcaatcat ttgtattgta ggtgttttag
      481 taatgttgct acccagcaga gcttgaacac ttgtggttag aagggagaag taaaaatgat
      541 agttgaagtt gagcaccaaa gacaaggtgt agatttaatc aaaattgata atgatgaaac
      601 taaaattatt tttacgaatt atggagcaag agtagtgtct tggaaatatc atgataataa
      661 tattgttctt ggaaatgttg ttgaagcgga tgaattttat catagtaatc cttttaaatt
      721 tggtgcttct ataggaagat atagcggtag aattgataat gctaaattta agttgaaagg
      781 taaagaatat cagttagaaa aaaacaacgg tgaacatcat ttacatggtg gatgtcatgg
      841 tttggacaat aagttatttg attatgaaat tagaaatgaa attgctcaaa ttaaagtgat
      901 atttaagacg gtgttgaaat cagctgatga tcatttcccg ggtgatatcg atgtgacgat
      961 tacacatatt tatgatgcgg atcaccaatg gtcgattgaa tacgaagcag ttgcatcaaa
     1021 agacacattg tttagtccta cgaatcatgt gtacttcaat ttgaatcgag ataataatgt
     1081 cgttgataat catcgcattt caagtaatca attagatatg tatgtattag atgagcgaaa
     1141 tattgttaca ggtgatattc tagatttaca tgaagtgttt gaagataaca agataaaatt
     1201 gagtgatatt tttacatcac aacatgcgca attaagccaa caaatgacac gttttggtgg
     1261 tctcgatcac ccatttacag ttggtgaaca taaaatgtat gttgaaaatc atgaatttat
     1321 gttagaggtc gatacagata tgcctcatgt tgtgttcttt acttttaacc agcctgatga
     1381 gtgggatagt ccatttaata tttataaacc acattctggc tttacattag aaacacaatt
     1441 tttaccaaat gatattaatt tatatggtga aaaagcaccg tcaattttaa aagctggtga
     1501 agcgttcaat tcgaaaacaa gtttccgtat cgaagaaaaa gtagaaaatg attaaaagta
     1561 tcggcattta tataatattt atgttatttg gcggaatttt gaataggtta tcaagaatcg
     1621 aatcaaatga agtgacatca ttaatataca gtttgaaata aaaaaatata taacataaaa
     1681 aaggaatcaa tagcatcagt agttataggt aagactatta tacaaacgat gtgtaagttg
     1741 attccttttg ttttgaaaag ttcatttaac acttataatg aaaggtaatt atttcaatgt
     1801 tctgttatac attttttgaa gtttatcgcg tcttgttggt gacagaaatg gattgtttag
     1861 tgcatatcgt aacagatctt gattttgtct atcattataa taaaggtcat taagctgttg
     1921 tacagataaa cgatgttcga ttggtgcttc ttgtattaag taaagctggt catttggaga
     1981 tacatagcct tgctttaata cccggaaata gaaacctgtt ttaccagatg tagacatgcg
     2041 cttcactaaa tcaggaatat tatattttgc ttgaattttc cagcatggtt ctctaatttc
     2101 agaaacctca attaacgcat cacctaaacg atattgatta ccaaagtaaa catcagattc
     2161 gtctaaataa tcaaatgtta aattctctcc aaacatcgca tgagtaggta gtgagggtaa
     2221 atcatcttga tacagtgcat agttgtgttt actaaaccca caaactgctt tatgtggtcc
     2281 accatgatct ttataagcct gttcatcacc agtgaaacca gttgaagata accatgttgt
     2341 ttgtgaaatc ttagttttat ctaaagcaga gcgcatcggt cttttcgagc tataaggcaa
     2401 atcttggatt tttcctgtcg aaattgcgtg aattgatatc atatcattct ccttaacgag
     2461 tattatttaa aatatattgt actataaaac atttttagaa ttgtaaattc tgattgatat
     2521 gtttaaaaaa atgttagaaa taagggtatg ttaatttata cgagtcatgt aaaatatgaa
     2581 ttgaatagat tacataaata tgggaggcta ccttcatgaa agatgtcaaa gcactcaagt
     2641 taatgacatt aaatgatgtt ttaagtcaaa tcaatggtga catgacgctg ggaattggta
     2701 caggaagtac aatggaatta ctcttacctc aaatggcgca actaattaaa gaacgtggtt
     2761 acaatattac aggtgtatgc acttctaata aaattgcatt tttagcaaaa gaattaggta
     2821 ttaaaatatg tgaaatcaat gatgttgatc atatcgactt agcaattgat ggtgctgatg
     2881 aagtagatcc atctttaaat ataattaaag gtggcggtgg tgcgctgttc agagagaaag
     2941 ttatagatga aatggcatcg cgttttgttg tggttgtcga tgaaacgaaa atagttcaat
     3001 acttaggtga gacgtttaag ttaccagtag aggtggataa atttaactgg tatcacatat
     3061 tacgtaaaat tgaatcatac gctgatataa aagtagaacg tcgtgtaaac gaagatgtcg
     3121 catttataac tgataatggc aattatattt tagattgtaa gttaccaaaa ggaattgacc
     3181 catataagtt ccatgagtat ttaattcatc tgacaggtgt gtttgagaca ggatacttct
     3241 tagatatggc agatcaagtt attgtaggca ctcaagaggg tgttaaaatt ttagaaaaat
     3301 aatatatagt atagaaaagc gcttgctagc atcatctttt taagaggtac tagcaagcgc
     3361 tttgttatta tttgttttta tctgaagatt gttcttcagt tttttcgata tgtttttctg
     3421 catcaattac aactgatgaa cgtctgtctt ctttataagt cgcaggttct tttaatgatt
     3481 gctctatttc atcttcattt gaatcgtttg ttaaatcgtc actttgtggt gcttgtttaa
     3541 ctgattctgc tgattctact gactccgaag tgatgtcatg atcttcatca tgattagatt
     3601 cagtttgatt acctatgtga tgttgaggtt ctgttttttc agtaccttca tgaatgcttt
     3661 gttcgtcagc cactgtattt tcagtatcat ttttagcaac accgaccgtt gatgcagtag
     3721 ctacacctgt tttttcagca tttttaatat ttgaagaaga tttttcagcc tcagtatgat
     3781 tagtttcttc atcgtcattg acatggtcta aataattatt aggctcaagt tcttcaaggt
     3841 ttcgtcttgt tgttaaatat gcaatacctc ggataattaa gcttaaacca atgtatccta
     3901 ctgcaacgat tgctgttgaa atggcgatga ctttgattga aaatagatcg ccgatttctt
     3961 cgctaaacaa gaaaataagt ccgaatgtca ttgaagcatg gaatgtcgtt gcaatataaa
     4021 ttgtacgtcc tttagtcgct ctaattaatt caccaagaat catagagaat gagaatgtat
     4081 aaaggaagtt ataagcagca aattctgtac cataagttgt atttgctgag aaaactgaat
     4141 acatcaaacc aacaacaata cttgcaaaga acgtgttcat tttcgtttca acaatatttt
     4201 gtaagtatga acggaatccg aattctacta cgaacgccat cagaatatgt ccaatcagaa
     4261 tgtgtgtaat aggtactgat aagcctgttg attgtaataa aataaagcta tctgcaaatg
     4321 tattaaagct gtacatacca ataattagaa taattaaagg taaaattaaa gctagcaata
     4381 gacgttcaat tactttcagg ctgattgaga attttaaacc tgcaagttgg acctttttat
     4441 atttgaaaac gagtatacaa atgattgcag caattaatgg tgctagatct gtaacttcaa
     4501 atataaagtg tttgacacca attatagact ggaaatctct gagcataatg gataacgcca
     4561 ttgtaatgac aaagaagacg aaaatcgtca ttgcccattg aaaaccagaa attttattgt
     4621 tcttcattat atgtaacctc cattaggtaa caagcaaaat atcttttagt aacaattata
     4681 catattacaa gccgagagat aaattgtcta tgtcaatatt aatgaaaatg taatagtctt
     4741 gacgtatttt attaacataa atcagctaag tattcgttat tacatagaaa ataggggtaa
     4801 aagtaatgta gataagtatt tttaagatac ggatttaact atgaattgaa ttcaaaattt
     4861 cctatacgtt atactttaat tgttaataag ccacataaat aagaggggga aatgctgtgt
     4921 acaagcaagg tgaaccaaat ttatggactg gaaggttaga tagtgaaaca gacccgaaaa
     4981 aatttagaca ttttcaaaca gtaacatttg aagatttgtc taagctggaa aagagtagta
     5041 tgccatcagg ggtcggtata ttaggctatg ctgttgacaa aggtgttgct ttaaacaagg
     5101 ggcgcattgg tgcaaaagaa ggaccagatg cgattaaaca agcatttgca ggtttgccgg
     5161 atttgaatca atgtgaaatt ttagtcgatt acggaaatgt ttatcatgat catgaggaat
     5221 taattgatac tcaaaaagaa tttgctatgc ttgcagcgaa gtcaattgct aatcatagac
     5281 aaacattttt attaggtggt ggacatgata ttgcgtatgc tcaatattta gcaacacgta
     5341 aagtctatcc aacacaatct attggtgtga taaatattga tgcgcatttt gatacacgtg
     5401 ctgaacaaca atctacatct ggaacgagct ttagacaaat tttagaagaa gatgaaaaca
     5461 cagattattt agtgcttggt attgctcaag gtggtaatac gcaaagttta tttgattatg
     5521 ctaaagagaa aaagattgat tatgtctttg cagatgaatt attgagtcac gtatcaccaa
     5581 caattaaaga tatgattgaa cgttttgtac atgaacatga tgtcattatg tttacgattt
     5641 gtatggatgt cattgatagt gcgtttgcgc ctggggtaag tgcgccggca gtgttaggtt
     5701 tgtatccaca tactgttctt gaattagcaa aacgtattat tccaagtgat aaggtgtctt
     5761 cagttagtat tgctgagatg aatccaacat atgacgctga caatagaact gctaagctcg
     5821 ttgctaattt agtgcatcat tttttaaaat aattaaaaat ttttatatgt gacgagattt
     5881 gcattgcagg gcagaatgtg agtatttctt aatagaagtt atattaacgt tgatatattt
     5941 gaaaaataat tgcagtaaga tgactcttaa gttgtagtca tcttactgct tatttatgtt
     6001 tgttgaaata gcaaaaataa caactgtatt gtttgaaaag ttctcttatt atataatcat
     6061 taattaaaat tttaacggag ttcaagaggc ctttttataa tgacgcctta tttgtaaaat
     6121 gtcatatgtg gtttagcctc tttataataa tttaatctgt cctcaagtgt gccagtatgt
     6181 aattctagct tatgaccatc agggtcggta aagtaaattg attgtctatc tctaatatct
     6241 ctaactcttc cttctaaaat attcacgtta ttatctttta atctctgatg ccaatattta
     6301 aattcgctgt catctatagt gaaagctata tgtgtatatg aaaagtgaat ttcattacgt
     6361 ggtatatctt tttcttcatt taaagcaatc cataggcctg caagctcaaa ataagcagtt
     6421 tttttaccag tcaacagcaa tttcccaagt aaaatatctc tataaaaatg tattgaatcg
     6481 tttaaatttc tgactgaaaa gcatatatga ttaatagatt ttaacatctg cttcacttct
     6541 ttcttagaat ttttataata aattattttt gtatcgattt ttgaaatgac gtcattaatt
     6601 tttatacgat gatct
//
LOCUS       DAHHYJ010000136         6589 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000136 DAHHYJ010000000
VERSION     DAHHYJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.136"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(75..3845)
                     /locus_tag="NBK22_002202"
     CDS             complement(75..3845)
                     /locus_tag="NBK22_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional autolysin"
                     /protein_id="HCD2415378.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVN
                     GDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSENQINPELIKS
                     AAKPAALETQYKAAAPKAATTSAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYK
                     PQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTING
                     EISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASF
                     ARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSH
                     NYSYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTGKLTVAANNGV
                     AQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVK
                     EGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQI
                     DKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPTPKPSTPTTNNKLTVSSLNGVAQINA
                     KNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVI
                     YNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIY
                     LFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNN
                     TGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNV
                     QNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVY
                     LYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYS
                     LKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVA
                     QIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISID
                     KINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNK
                     VVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQ
                     NTLYKMRWNPAHPGTHQYATDIDWANINAKIIKGYYDKIGEVGKYFDIPQYK"
     gene            complement(4073..4507)
                     /locus_tag="NBK22_002203"
     CDS             complement(4073..4507)
                     /locus_tag="NBK22_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD2415379.1"
                     /translation="MFSKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYEYESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAKDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(4662..5132)
                     /locus_tag="NBK22_002204"
     CDS             complement(4662..5132)
                     /locus_tag="NBK22_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCD2415380.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSHDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(5180..6397)
                     /locus_tag="NBK22_002205"
     CDS             complement(5180..6397)
                     /locus_tag="NBK22_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCD2415381.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQ
                     APQASNNQNGVVN"
BASE COUNT         1889 a         1079 c         1096 g         2525 t
ORIGIN      
        1 tataaaaaag ggatcaaagg tcatccccca tgattgatag tgggggatga cttttgatcc
       61 tatgttcatg ttgcttattt atattgtggg atgtcgaagt atttgccgac ttcgccaatt
      121 ttatcatagt agcctttgat gattttagca ttgatgttag cccaatctat atctgtagca
      181 tattggtgtg ttcctggatg tgcaggattc catctcattt tgtaaagcgt attttgacca
      241 gcttttacat atgagttgcc gatgaattta gcaccaccaa cgattgcttt tgatactgtg
      301 tcccaaccag cttgtttagc atatttaata ccttcacgta aaggatcgtt atcatatgca
      361 gcaataccaa atacgttatg gtatttcgtg tttgagttag ttacaacttt gttgttcact
      421 acatctgcac ctttcgctaa ttgagaagta ccgttacctg tttctaatag ggcatgtgag
      481 ataagataaa cttcattaat gccatacatt tgagcagctt tgttaaatgc agcaccttgg
      541 ttttctaata cacctttacc ttttaagaat tgattaattt tatcaataga aatattttgt
      601 ggttggtcta agcgtaagaa ttgatatttt aatgctggat cttgagctaa acgcttcgta
      661 tccattgcat gcttaacatc attaaagtta gcatctgtcc acttacctgg tacacgttgt
      721 acttgtggtt tatattgtaa accagcttgt atttgagcaa cttggtttaa tgtcatacct
      781 gtttgattat acttaattaa ttctttagct aaatcagttg atttaatcca tgctaattta
      841 ccgttagata atttaccata gtaccaagtt tgtccattaa tgacttgttc tttaacaact
      901 gcgaatggtt gttcattaaa tgcttttaat gagtatttag ctgtatcaga atttggtgtt
      961 acatagtaat aaccattacc atttttaatt acataagtgt agttataatc tttggcagct
     1021 gatgtagttg gtttaacagc agttggtgca gttaaatctt ttgcatttac ccaaccagtg
     1081 cggttattaa tagtaccgta taaataaaca tctttgccta cagatacttg tttcgttgca
     1141 ttaaatgtac cttgagcaat gttattgcct gttaaaatga cttggttttt agtaccccaa
     1201 ggaaccattg ataagccgtt atttgattta ttaacagtat atttttgagt cgttttaact
     1261 tctttgccta agttttgaac atttaagtct tttacattga accaacctaa tgggatgtta
     1321 tggcttgtat tgtttaataa tacatacgtt tcattaccat gagcacgctc ttttgttaca
     1381 tagaacgtac ggtctgcata tttcgcaccg tttttcgctg ttttttcata aacagaagca
     1441 cgaataccag tgttgtttgg tttaacttga gcaatcttgc taactgtttg agtcgtttgt
     1501 ggtttagtaa cagtataagc ttttacagct gtttttggtt gtgctactgc ttttttaggt
     1561 gcagcaggta cagctaaata tgctttactt acccaaccag atttaccatt tacagttcca
     1621 aataaataga tagatttatc aatttgttgt tgcttagtcg ctttaaaagt ttggttacct
     1681 gtaccagaaa ctgcaccagc ttcttgttta taagtgcccc aaggtactga atataattta
     1741 gtgcctggtt ttactgtata tgtttgcatt acatttacag gtgattttgc attgttataa
     1801 ataacgtcac cttgtttaac ccaaccaatt aaagttggac tattgtaatc tttaactaag
     1861 tagaatttgt ttccacctaa acttgcttct tttgttacag caaatgtttt ttgaacttct
     1921 ttcgttggct taccagtttt gtcataaact gtagtgaata agccattgtt tttagcatta
     1981 atttgagcaa caccgtttaa tgatgaaact gttaatttat tatttgttgt aggtgttgat
     2041 ggcttaggtg ttggtgtagg cgtaggttta gcagtatcaa ctaaatatgc tttacttacc
     2101 caaccagatt taccattcac agagccatat aaataaattg atttatcaat ttgttgttgc
     2161 tttgaagcct taaatgtttg gtttccagag ccagacacac taccagcaac ttgtttagat
     2221 gtaccccaag gtactgtata aagtttcgta ccaggtttga ttgaatatga ttgatttaca
     2281 tttacaggtg atttagctgt gttgtaaacc acatcgcctt ctttaaccca accaaattta
     2341 ttaccagaat tgtaatcttg aacaagatag aatttttgat tacctaatgt agctgtttta
     2401 gatacagcaa atgttttttg aacttcatta gttgctttac cagttttgtc gtatacagta
     2461 gtatataaac cactatttgt tggtttgatt tgtgcgacac cattgtttgc agcaactgtt
     2521 aatttaccag ttgatggttt cgacggtgtt gttggttttg atggtgtagt aggggtagtt
     2581 gtagattgcg taccccatgg cgccacttta cccattttta ttaaatattt ttcattaatt
     2641 aagtcatata attgatcata actataatta tgacttctta aatatccatg tggatcggca
     2701 tggtcagtac cacctaaata tttacttaca gcgtagtgag tccatactgt accatttcca
     2761 tcatactcag cactgtctgg ttttaaacca taatattgta attgtgtagc tgcatagtca
     2821 gcatagttat tcattgaacg tgcaaatgaa gcatagtcgt gtgtgtgtac gatttcaaca
     2881 ttgatgaatc tagggttacc gactgcaccg acaccccaag ataagtaatc cgttggtgct
     2941 gtttcgatta tacgatcccc atcaacaaat gcatgtacga atgcgttttg atagttattt
     3001 ttcatataac taatttcacc atttatcgtc gaacgatcat tagctgtatc atgaactacg
     3061 ataccttcag gacgacctac gccgttacgg tatgcgtatt tagggaagta agatgtataa
     3121 tcttcttcaa ttttaggtgc ttttaagtta tttttacgaa tgtaatcgtt aattgaagag
     3181 tttacttgtg gtttatattt tggcaaactc gtttttggtg ttgcagcaac tgatcttggt
     3241 tgtgctgaag cgctaaaagt agttacttta ggtgtcgctt cagttttagc tttaggtgct
     3301 gatgtagttg cagctttagg tgctgcggct ttatattgcg tttcaagagc tgcaggttta
     3361 gcagctgatt taattaattc tggattaatt tgattttctg aattatcatc ttcatcatca
     3421 actaaactat aaccagcatt tgtaacatta gtgttagttt taggtgctgt agtgcttgtt
     3481 gactttgcaa caggctgcgt attatttgta gtcgctgatt gattagcacg agtgtcacca
     3541 tttacttgtg cagtatcaac tttttgactt acttgagcat tgcctgtttt gttatttgct
     3601 gtttttggtt ggacaatagc agggtcttga tatacttgag tgccagaaat gttttgcgtt
     3661 ggatttttta cctcagcttt tgcttgttca gtagttgctt taactttatt actatctaaa
     3721 acgtttttat tagtagtttg atcttgtgtc gtctcagctg cttgaacttg atgtgcagtg
     3781 actgctgaac ctacaagcgt taatgcaacc attgatggta gtttgtaatt gaattttttc
     3841 gccattctat ttattactcc taacatttat taattattac taacattata gtacctgttt
     3901 tatatacctg tgcgtattta accaatttgt aatatactct taatttacat tttcactgta
     3961 aaaaaatgaa aaaaattttg aatctcaatg gtgacgtgac aaatcaaata gaattgatgt
     4021 caatttaaga attggtttat tacaaatgtt tctaaaatag atacaacttt ttttaattaa
     4081 gcgaggttaa cttttttgtc atttcaatat gctcgatgcc ttcctcaaga aatatattac
     4141 ctctcatttt aaagtttaaa ctttcataaa atgggatagc atgacattgg gcattcatag
     4201 tagctacgta aaaaccttca tctttagcta atgattctac agcttgcata agcattctac
     4261 ccattccttg tccacgatgt gatttcatca cagctactcg ttctattttg acagttgttt
     4321 cattaatagg gcgtattcga gcagtggcaa ctggctgtcc attatcatat ccaatgaggt
     4381 gaatagattc atattcatat tcatcaattt cactttcctc agggacgcct tgttcttcta
     4441 caaacacttt ctttcttata tagaagcaat cttctaacat cttttgattg tttacttttg
     4501 aaaacataaa attcctccac atcctaacaa aatcatttac atttaattta ctaataataa
     4561 catagaataa cgattgttat tttcctcaaa aataattaga cataaaaaag ctataaagat
     4621 gattgttgtg aatcatcttt atagctaatt aattattaat gttatagtgc ttcttttcta
     4681 ataattgctg tatattgcca aaagattcgc atttgagcaa tattccaatt attcattccc
     4741 attgtaaatc ctgaaggctt aaatatattc acatcagtta cttctaaagc tgctgcaact
     4801 aaatattgta atggaatgct aagtgatttc attgtttcgg taatgccatt ttcatcatat
     4861 acccatgaaa atggtaactc tgattcgaaa acatcaacac tattgaaaat ttcaggtagg
     4921 gctaaaatat aacatgcacc atctacaata ggattgtcga tactattttt gtaataaatt
     4981 aggagtgctt catagttttc tcgatgctca tgattgacgt aaaaaaattc acttctaaaa
     5041 tgagccatat catggctatg aatgatttgt tgttctaaca tattaaacat gccatttata
     5101 tttgcaatct tttcgtatgt ttttctactc atgtatgatg ctcctttcat aaatagtaaa
     5161 tgttaatcgt catataatat taatttacaa caccattttg gttatttgaa gcttgtggcg
     5221 cttgttgtgt gccaccttga ttttgatttg agttttgatc tgtagcaggt tgttgttgat
     5281 tgctggaatc actgttatta gttgaatcac tgttctcgtt agatgtctta tctttatctg
     5341 tcgtatcatt ttgttctttt ctcaataaac tattatctaa aggcgttaat ggtattaatg
     5401 aaccataatg attaatgaca cgttgatcta agaaatcatt tttatcatta ataggtgata
     5461 attctaagtc tttacgaagt aagtttgcat atttttgaat gctttcaaca cttggatgat
     5521 aatagtaaat accatttaac atatcatctt taccttttaa ttgcgcagtt ttaatttcaa
     5581 catcatttgt taagtacatt tttgctaaag ctttaatttc agagttagtt aaattatgct
     5641 ttgcattttt acctacaatt tgaatcacgt tatcaagttt atcaatagaa tcaacttcct
     5701 gtgctttttg gaataaaatc ttaattaatt ccatttgacg ttgtccacgt tttaagtctg
     5761 aatcatgatg tctagttcta gcaactgcta aagcctcatc accatttaat ttttggtacc
     5821 cttttttaat tttaatctta ccagtatcat ctgtgttagg ttcatttaag tcgtatggca
     5881 catcatagta tatgccacca agctcattta cagcctcgac aaatgctttc atattgactc
     5941 tcacataata atcaacaggt acattcatgg tagcttctac cgaatccatt gcggcaattg
     6001 gaccaccata tgcatgtgca tgggtaatct tatcgtaata gccaacttta ggaatgtagc
     6061 tgatagtatc acgtggaata ctaagcattc taatttgatg ttttgattga ttaaaagtag
     6121 ttaaaatcat agcgtctgat ctagagtgtt cagcatcctg tccttttttt cttcttccat
     6181 cgttatcatc gatacctaag aaaagaatag agataggttg ttcttcggga ttgactttat
     6241 tatctcttaa gttggattga cgattagcat ttttgctgtc ttgagaagat tcgaatgcat
     6301 cttgggacgt tttaaaaagt aacgtagcga agactattgg aacaacaatg agaaccaatg
     6361 ctagaaggat caaaaagtat tttaaaaatt tattcatgat tgatgctcct atattaaatt
     6421 tttgttaaat cataatcata attttaaaat ataattagtt gaacgatatt tatacaactt
     6481 cattactaat acattatagc cttaaattgt agtactttat agtaaaaaat acaaatgtta
     6541 tgtcaaagtt ccttgtaatt tgaatgtttt gttaaaatca aaaagcatt
//
LOCUS       DAHHYJ010000137         6553 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000137 DAHHYJ010000000
VERSION     DAHHYJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.137"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            91..1320
                     /locus_tag="NBK22_002206"
     CDS             91..1320
                     /locus_tag="NBK22_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCD2415382.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GVPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
     gene            1515..2372
                     /locus_tag="NBK22_002207"
     CDS             1515..2372
                     /locus_tag="NBK22_002207"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCD2415383.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
     gene            2372..3316
                     /locus_tag="NBK22_002208"
     CDS             2372..3316
                     /locus_tag="NBK22_002208"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCD2415384.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMAHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDVEQQA
                     LAIKSAFVAQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            3585..4553
                     /gene="pfkA"
                     /locus_tag="NBK22_002209"
     CDS             3585..4553
                     /gene="pfkA"
                     /locus_tag="NBK22_002209"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCD2415385.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            4575..6332
                     /gene="pyk"
                     /locus_tag="NBK22_002210"
     CDS             4575..6332
                     /gene="pyk"
                     /locus_tag="NBK22_002210"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCD2415386.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKDLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVSNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
BASE COUNT         2313 a          882 c         1344 g         2014 t
ORIGIN      
        1 cagatttacc caatattttt acttttataa tgtaatgcgt tttatccaag ttatttttta
       61 aaaataaata ttgaattagg ggctgatttc atgtcattaa gagatgaagc attggaaatg
      121 cacaaacgta atcaaggtaa attagaagtt aaaccaaatg taaaagttac taataaagag
      181 gaattaagtt tagcatactc acctggcgtt gctgaaccgt gtaaagatat ttatgaagat
      241 aaaagaaaag tatatgatta cacaattaaa ggaaatacag ttgcagttat tactgatgga
      301 acagcggtat taggtttagg taacattgga cctgaagcaa gtattcctgt aatggaaggt
      361 aaagcagtat tattcaaaag cttcgctggt atcaatgggg tgcctattgc gttaaataca
      421 actgataccg aagaaatcat taaaacagtt aagttgttag aacctaatta tggtggtatt
      481 aatttagaag atatttcggc accacgttgt tttgaaattg aagaacgatt gaaaaaagaa
      541 actaatattc cggtattcca tgacgatcaa catggtacag caattgtaac aatggcaggt
      601 ttggtaaatg cattgagagt tgttaacaaa gatattgcta aaataaaagt tgtactaaat
      661 ggtgctggtg cagcaggaat agccattgtt aaattactat acgcgtatgg tgtaagaaat
      721 atggttatgt gtgactcaag aggcgcaatt tttgaaggac gttcatatgg tatgaatcct
      781 acgaaagatg ttgtagctaa atggacaaat aaagataaga ttgaagggtc tttagaagaa
      841 gtcgtaaaag acgcagatgt atttatcgga gtttctgtag ctaatgcgct gtcacaagat
      901 atggttaaga gtatggcaga taatccaatt atatttgcaa tggctaatcc aaatcctgaa
      961 ataatacctg atgatgccaa agcggcaggt gcacgagttg ttggtacagg acgttcagac
     1021 tatcctaacc aaattaataa tgtattagct ttccctggta tttttagagg tgcattagag
     1081 gttgaagcta cacatataaa tgaagaaatg aaaaaggcag ctgtagaagc gattgctgat
     1141 ttaatcgata gttctgaatt aaatgaagac tactgtatcc caggaccgtt tgataaacgt
     1201 gtagcgccat cagttgctcg taatgttgct aaagcggcaa tggaatctgg agtagctagg
     1261 attgaagttg atccgcaaga tgtgtatgat aaaacaatga aacttacaga tttacaataa
     1321 aaataattaa cgtttaatta aattgcttaa aagttagcaa taaagagtca ttaaaatgat
     1381 gaaaatgaaa gtttatgata aacattcaac agtcaaacga atataaatca aataaattta
     1441 aacccgtttt taactggtca agttcagttt aaggctctaa atggttagaa cagaggttat
     1501 ttggaggttt tcctatgttt aaagattttt ttaatcgaac aaagaaaaag aaatatctta
     1561 cagtacaaga ctctaaaaat aatgatgtgc ctgcaggtat tatgactaag tgtccaaagt
     1621 gtaagaaaat tatgtacaca aaagaattag ctgaaaattt aaatgtgtgc tttaattgtg
     1681 atcatcatat tgctttaact gcgtataaac gtatagaagc aatttctgat gaaggatcat
     1741 ttacagaatt cgataaggga atgacctctg cgaatccatt agattttcca agttatttag
     1801 aaaaaattga aaaggaccaa caaaagacag gtcttaaaga agcagttgtg actggtacag
     1861 cgcaactaga tggtatgaaa tttggcgttg ctgtcatgga ttcacgtttt agaatgggaa
     1921 gtatgggatc ggttatcggt gaaaagatat gtcgcatcat tgattactgc actgagaacc
     1981 gtttaccatt tattcttttc tctgcaagtg gtggtgcacg tatgcaagaa ggtattattt
     2041 ccttgatgca aatgggtaaa accagtgtat ctttaaaacg tcattctgac gctggactat
     2101 tatatatatc atatttaaca catccaacta ctggtggtgt atctgcaagt tttgcatcag
     2161 ttggtgatat aaatttaagt gagccaaaag cgttgatagg ttttgcaggt cgtcgagtta
     2221 ttgaacagac aataaacgaa aaattgccag atgatttcca aactgcagaa tttttattag
     2281 agcatggaca attggataaa gttgtacatc gtaatgatat gcgtcaaaca ttgtctgaaa
     2341 ttctaaaaat ccatcaagag gtgactaaat aatgttagat tttgaaaaac cactttttga
     2401 aattcgaaat aaaattgaat ctttaaaaga atctcaagat aaaaatgatg tggatttaca
     2461 agaagaaatt gacatgcttg aagcgtcatt ggaacgagaa actaaaaaaa tatatacaaa
     2521 tctaaaacca tgggatcgtg tgcaaattgc gcgtttgcaa gaaagaccta cgaccctaga
     2581 ttatattcca tatatctttg attcgtttat ggaactacat ggtgatcgta attttagaga
     2641 tgatccagca atgattggtg gtattggctt tttaaatggt cgtgctgtta cagttattgg
     2701 acaacaacgt ggaaaagata caaaagataa tatttatcga aattttggta tggcgcatcc
     2761 agaaggttat cgaaaagcat tacgtttaat gaaacaagct gaaaaattca atcgtcctat
     2821 ctttacattt atagatacaa aaggtgcata tcctggtaaa gctgctgaag aacgtggaca
     2881 aagtgaatct atcgcaacaa atttgattga gatggcttca ttaaaagtac cagttattgc
     2941 gattgtcatt ggtgaaggtg gcagtggagg tgctctaggt attggtattg ccaataaagt
     3001 attgatgtta gagaatagta cttactctgt tatatctcct gaaggtgcag cggcattatt
     3061 atggaaagac agtaatttgg ctaaaattgc agctgaaaca atgaaaatta ctgcccatga
     3121 tattaagcaa ttaggtatta tagatgatgt catttctgaa ccacttggcg gtgcacataa
     3181 agatgttgaa cagcaagctt tagctattaa atcagcgttt gttgcacagt tagattcact
     3241 tgagtcatta tcacgtgatg aaattgctaa tgatcgcttt gaaaaattca gaaatatcgg
     3301 ttcttatata gaataatcaa cttgagcatt tttatgttaa atcgatactg ggttttacca
     3361 taaattgaag tacattaaaa caataattta atatttagat actgaatttt aactaagatt
     3421 agtagtcaaa attgtggcta ctaatctttt tttaattaag ttaaaataaa attcaatatt
     3481 taaaacgttt acatcaattc aatacattag ttttgatgga atgacatatc aatttgtggt
     3541 aatttagagt taaagataaa tcagttatag aaaggtatgt cgtcatgaag aaaattgcag
     3601 ttttaactag tggtggagat tcacctggaa tgaatgctgc cgtaagagca gttgttcgta
     3661 cagcaattta caatgaaatt gaagtttatg gtgtgtatca tggttaccaa ggattgttaa
     3721 atgatgatat tcataaactt gaattaggat cagttgggga tacgattcag cgtggaggta
     3781 cattcttgta ttcagcaaga tgtccagagt ttaaggagca agaagtacgt aaagttgcaa
     3841 tcgaaaactt acgtaaaaga gggattgagg gccttgtagt tattggtggt gacggtagtt
     3901 atcgcggtgc acaacgcatc agtgaggaat gcaaagaaat tcaaactatc ggtattcctg
     3961 gtacgattga caatgatatc aatggtactg attttacaat tggatttgac acagcattaa
     4021 atacgattat tggcttagtc gacaaaatta gagacactgc gtcaagtcac gcacgaacat
     4081 ttatcattga agcaatgggc cgtgattgtg gagatctagc attatgggct ggattatcag
     4141 ttggtgctga gacaattgta gttccagaag tgaaaacaga tattaaagaa atagctgata
     4201 aaattgaaca aggtattaaa cgtggtaaga aacactcaat cgttcttgta gcagaaggtt
     4261 gtatgactgc gcaagattgt caaaaagaat tatcacaata catcaatgtt gataatagag
     4321 tgtctgtgtt aggtcacgtt caacgtggtg gtagcccaac aggtgcggat agagttttag
     4381 catcacgttt aggtggatat gcggtagact tattaatgca aggtgaaaca gctaagggtg
     4441 ttggaattaa gaacaataaa attgtagcaa catcttttga tgaaattttt gatggtaaag
     4501 atcataaatt tgattatagt ctatatgaac ttgctaacaa gttatctata taagatttca
     4561 ggaggaatta taaaatgaga aaaactaaaa ttgtatgtac aattggacca gcttcagaat
     4621 cagaagaaat gattgagaaa ttaatcaatg ctggtatgaa cgttgcacga ttaaactttt
     4681 cacatggtag tcatgaagag cataaaggta gaattgatac aattcgtaaa gtagctaaaa
     4741 gattagacaa aattgtagca attttattag atacaaaagg tccagaaatt cgtacgcata
     4801 atatgaaaga cggtatcatt gaacttgaac gtggtaacga agttattgtt agcatgaatg
     4861 aagttgaagg aacacctgaa aagttctcag taacatatga aaacttaatt aacgatgttc
     4921 aagtaggttc atacatttta cttgatgatg gcttaattga attacaagtt aaagatattg
     4981 accatgctaa aaaagaagtt aaatgtgata ttttaaactc tggtgagctt aaaaacaaaa
     5041 aaggtgttaa cttacctggc gtaagagtaa gtttacctgg tattacagaa aaagatgctg
     5101 aagatatccg tttcggtatt aaagaaaatg ttgacttcat tgcagcaagt ttcgtacgtc
     5161 gtcctagtga tgttttagaa attcgtgaaa ttttagaaga acaaaaagct aacatttcag
     5221 tattccctaa aattgaaaac caagaaggta ttgataatat tgcggaaatt cttgaagtgt
     5281 ctgatggttt aatggttgca cgtggtgaca tgggtgttga aattccacct gaaaaagtac
     5341 caatggttca aaaagattta atcagacaat gtaacaaatt aggtaaacca gttattacag
     5401 ctacacaaat gttagattct atgcaacgta acccacgtgc tacacgtgca gaagctagtg
     5461 acgttgccaa cgcaatctat gatggtacag atgcagtaat gttatctggt gaaactgctg
     5521 ctggtttata tcctgaagaa gctgttaaaa caatgaggaa tattgctgta tcagctgaag
     5581 cagcccaaga ttacaaaaag ttattgtcag atcgtactaa attagttgaa acttcattag
     5641 tgaatgctat cggtatttcg gttgcacata cagctttaaa cttaaatgtt aaagcaattg
     5701 tagctgctac tgaaagtggt tcaacggcac gtactatctc taaatatcgt ccacattcag
     5761 acattattgc ggtgactcca agtgaagaaa ctgcacgtca atgttcaatt gtttggggag
     5821 ttcaacctgt agttaaaaaa ggacgtaaga gtacagatgc attgttaaac aatgcagttg
     5881 caacagctgt tgaaactggt agagtatcta atggtgattt aatcattatt actgctggtg
     5941 taccaactgg tgaaactgga actactaata tgatgaaaat ccacctagtt ggtgacgaaa
     6001 ttgctaatgg tcaaggtatt ggacgtggat cagttgttgg tactacgtta gttgctgaaa
     6061 ctgttaaaga tttagaaggt aaagatttat ctgacaaagt tatcgttact aactcaatcg
     6121 atgaaacgtt tgtaccttat gtagaaaaag ctttaggctt aattacagaa gaaaatggta
     6181 ttacatcacc aagtgcaatt gttggtttag aaaaaggtat tccaacagtt gtaggtgtag
     6241 aaaaagctgt taaaaacata agcaataaca tgttagttac gattgatgct gctcaaggta
     6301 aaatctttga aggatatgca aacgtactat aatttataaa aaaacgcctt tccatttatc
     6361 aacaatggaa aggcgttttt tggttcatct ggtattttat gacgtaatta ataggttatt
     6421 tgataatgat agtgtatgaa tggcaatcta tataaatgtt tatatctttt atacatgtac
     6481 attatcacct tcaaactttc actcatatta ctttggaaat ttattataaa atagaagtat
     6541 ggatgtattt ctg
//
LOCUS       DAHHYJ010000138         6477 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000138 DAHHYJ010000000
VERSION     DAHHYJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.138"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(143..451)
                     /locus_tag="NBK22_002211"
     CDS             complement(143..451)
                     /locus_tag="NBK22_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415387.1"
                     /translation="MTVNIVVSIVFCISMVILGIYVAITKDFTLISYINQTTIADKHK
                     NQIAYIFALCISLSAVFLMSAILCFEYDFIALAFLFLTIALLLIALFYVYFYKITKYP
                     "
     gene            complement(595..861)
                     /locus_tag="NBK22_002212"
     CDS             complement(595..861)
                     /locus_tag="NBK22_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415388.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(1145..2953)
                     /locus_tag="NBK22_002213"
     CDS             complement(1145..2953)
                     /locus_tag="NBK22_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCD2415389.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWDVFLYNSLHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLSRYLNAQTNNPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPSSKQNTSYIKPFPKSVTDDYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLKSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDQAVELAN
                     KSHADLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLARYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGLEGKKKI
                     ESIMTHFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            3085..3477
                     /locus_tag="NBK22_002214"
     CDS             3085..3477
                     /locus_tag="NBK22_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCD2415390.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEEMSLPLMWEFPGGKIEKNE
                     TEKEALIREIREEMKCDLIVGDKVITTEHEYDFGIVKLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            3479..6340
                     /locus_tag="NBK22_002215"
     CDS             3479..6340
                     /locus_tag="NBK22_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCD2415391.1"
                     /translation="MSRLLNDFNQSLKKGFIDKDISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFELLWQKSTPLTQQWIKSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERINYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEEDIDTSLRILDF
                     SFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFKIFLEDLIELSKYNNEKYQK
                     GKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIRSQEMPIFITYDKHEDISDSTKY
                     EDEFLSQDELKWFTKSNRTLKSKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTA
                     GYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN"
BASE COUNT         2428 a          836 c         1077 g         2136 t
ORIGIN      
        1 atcaatttgt tttgtgatat taaaaagcat aaatgattct atattaatgc aactttgtat
       61 tcgtacaagg ttgtattttt tcgcaaacaa ttacttactg acagcgcacc attaaatcta
      121 accaacacac tatcacaacc atttacgggt attttgtaat tttataaaag taaacgtaga
      181 ataatgcgat aagtaataat gctatcgtta aaaataagaa tgccaatgca ataaaatcat
      241 attcaaagca tagaattgca gacattaaaa atactgcact caaactgata catagcgcaa
      301 atatatatgc aatttgattc ttgtgtttat ctgcaatggt cgtttgattt atataagaga
      361 ttagtgtgaa atctttagtt attgcgacat aaatgcctaa tataaccata ctgatacaga
      421 acacgataga aactacgatg ttgacggtca ttgttgcacc ttcctgatag aatataatgt
      481 actgtctttt agtaatggat gcgtttctta tatatgtatg tttctaaaaa acagtacgtt
      541 tttatcattt tttaatttat tagttgaatg aatggggtta tgaaaaagga agttttaatc
      601 ttttgtcatg aaataaatgg gatgaatatc acgactagaa gtaatgttac gaacaggagc
      661 gtataaatct agagacgcta aattcgacat agtatgttgc tcaattatgg ctgatgatga
      721 atttaaagta tgtgcgttgg aactgtcgga tttttgttca taatgttttg catattgcgc
      781 catgatgaat agtgtaaaaa taaataaaac aataagagat ataatgccca taatcaaaag
      841 tatttgttta gagcctttca ttatttcaca tcctttctaa aatatatttg taactaaatt
      901 taaaatagtt atttttgtaa ttctaaacct ttttcatcgc gaaaacaatt aaataggtcg
      961 cggtattaat tattatatta ttaccgctta atatgaaaaa tacatgaaaa ttaattttct
     1021 aatatacttt tgaaaaatta ttacaaatta gccccttcaa aacgcgaaaa cataaggatt
     1081 ctagtttcaa aagggctgat aagtataaaa tgaaatgtaa tatttcgatg tttaaaattt
     1141 ttaattagct aaaaatcatc gcattaattt tttgagctac atcatcaaaa ttcggacatt
     1201 ttaacgacac atataattta attttaggtt cagtaccaga aggacgtaaa gcgataaatc
     1261 cttcgtcaaa taagacacga ataacatttg atttaggaga attaatctgc gacgttgtgt
     1321 ctttatccaa atgataaacc tcgctagtta aataatcttc aattgctttc actttcagtc
     1381 cctgaatctc ttgcggtgga tttgaacgga aatgtgtcat aattgattca atttttttct
     1441 taccttctaa tccttctagc gtatgcgaga ataatgtatc ctcatgtcta ccaacagttt
     1501 gataaatctg ttctaattca tctttcaatg ttttgccata taattttaac tcagaagcgt
     1561 attttataat gagtggcaca atttgtacgg catctttatc acgtacaaaa ggctctgata
     1621 gaaaaccgta actctcttca aatgcgaaaa tcatattttg atgatcatcc agttgtctta
     1681 tttcctgagc aataaattta aagcccgtca gcacctcttt gtattcaaca ttattatagc
     1741 gtgctaatga ctttgttaac tcactactta caatagattg aatcattaag cggtgacgta
     1801 attgagacgt ttgttgaata cgataattaa gtaacagcgc accaatttga ttgccattaa
     1861 aatacgtgat atgaccatgt gcatcacgtt cagcaatgcc aagacgatcc gcatcagggt
     1921 cagttgatat taataagtct gcatgactct tatttgcaag ttcgactgct tgatcaaaag
     1981 cgcgatgatc ttcaggatta gcactttgaa ctgagctaaa attcggatca ggtttacatt
     2041 gtgcttctac aagattaaat tgattgaaat tcaaagattt taataactca ggtacaatcg
     2101 gaacacttgt accatgtaaa ctagtgaaca caacttgtaa atcagattta ggaatatagc
     2161 caatcatatt ttgaatgtgt ttcatatagt catcagttac agatttcgga aatggcttga
     2221 tataagatgt attttgtttt gaagaaggta tgtcaatctg taatggatcg ccaacttctt
     2281 cgatataacg acttgcaagc tcagatgcat cagttgatag ttgcgcgcca tcagaaccat
     2341 atactttaat gccgttatag tctttcggat tatgacttgc tgtaatcata atgccagcag
     2401 tagtattaag attgcgcacc gcgaacgata attccggtgt cgttttatat gtatcaggta
     2461 aataaactgt tatttgatgg tttgctaata cattagcaat aatttgggcg aattcagttg
     2521 aaagatgtct aatatcataa tgaatgacta ttgttggatt gtttgtttgt gcatttaaat
     2581 aacgcgataa acctaatgcc aatttttcaa tagtaaactt attaagtcga ccttcaccaa
     2641 gaccgaattt tccgcgtata cccgcagtac caaatgttaa tttactttca aaaccttctc
     2701 gctgttcaat gtcagattgc tgctcataaa aatgtttaac taaactatca ttagctcttt
     2761 ctatccataa ttctttatcc attgttgcta aacatccttt caaaatctca gttagactta
     2821 ataaaacatg aaaactaaag cccttacatt tatgtaatga attataaaga aatacgtccc
     2881 aaaagtaaaa aaacacagcc ccaagacaat acttttcaca agtattatat aatagatgtg
     2941 tatgaaaatg catggagtag atgtaagagt gatatatcat gcaggaaatt gtaaaatatt
     3001 aatttttaat attgatttat atatagtttt gtaatttttg atagaataac aattaagatg
     3061 attttaaatt gaagggtgag tcttatgaaa aaagtaatca atgtagtagg agctattatt
     3121 ttttctgata acaaaattct ttgtgcacag agaagtgaag aaatgagtct acctttaatg
     3181 tgggaatttc ctggcggtaa gattgaaaag aatgaaactg aaaaagaagc tttgatcaga
     3241 gaaattagag aagaaatgaa atgtgatcta attgttggag acaaagtcat aactacagaa
     3301 catgaatatg attttggcat tgttaagtta acaacataca aatgtacttt aaacaaagag
     3361 ttaccaactt taactgaaca taagagtatt gaatggttgt caataaacga acttgataaa
     3421 ttaaattggg ccccagcgga tataccagcc gttaataaaa ttatgactga gggataatat
     3481 gagtagatta ctaaatgatt tcaatcaatc tttaaaaaaa ggttttatag acaaagatat
     3541 ttctcataaa gggaattata caccgaagct tttagtaaat aataaaaatg aaaaagtcct
     3601 ttcaacaatt atagatgaac ttcaaaaatg tgaaacgttc tatttttctg tagcttttat
     3661 aacagaaagc ggattagcca gcttaaaggc tcaactatta gatttaagca ataagggtgt
     3721 taaagggaaa atattaacat ctaattactt aggatttaat agtcctaaaa tgtatggaga
     3781 attacttaaa ttaaaaaatg tagaggtaag attaactgat attgctggat tccatgccaa
     3841 aggatatatt tttgaacata aggattatag ttctatggta ataggaagct ctaatttaac
     3901 atctaatgcg ttaaaggtta attatgagca taatgtttta ctgtctacta tgaaaaatgg
     3961 cgatttagtt gatagtgtaa aaaatgaatt tgaattgtta tggcaaaaga gtaccccact
     4021 gactcagcaa tggattaaat catataaaga atcatttgag taccgctcct tagaaaaatt
     4081 agcggaggtt gaacaaactc aaatgctgct agctgataag gtgaaaaaat cagtggaaat
     4141 tgtacccaat ttaatgcaag cagaagcatt aaggtcatta aaagctatta gggataaagc
     4201 caaagataag gcgttaataa tatctgcaac tggtacaggt aaaacgattt tatgtgcatt
     4261 agatgttaga gaagtaaacc ctaataaatt tttatttatt gttcataatg aggggatttt
     4321 aaatagagct aaggaagaat ttaaaaaagt attacctata aaaaatgata gtgattttgg
     4381 attgttgaca ggaaaacata gagatgtcga tgccaaatat ttatttgcaa ccattcaaac
     4441 attatctaga gatgataatt ttaagcaatt tgatgaaaat gaatttgatt atattgtttt
     4501 tgatgaagca catcgttctg ctgcatctac ttatcaacga gtatttaact acttcaaacc
     4561 taagttcatg ctgggaatga ctgcaacacc agaaagatca gatgaattaa gtatatttga
     4621 actgtttgat tataatattg cgtatgaaat aaggttacaa gcagcattag aaagtgatat
     4681 tttatgtcca tttcattatt ttggtgtgac tgattatgta catcaaggta ttaaagaaga
     4741 tgatgtaact aaactgagat atttaacttc tgatgaaaga attaattata ttattcaaaa
     4801 gacagattac tatggatatt caggtgaaat tttacaagga ttaatttttg taagtagcaa
     4861 aaaagaagct tatgatttag cggataaact aagttcaaaa gggattaaaa gtgttgcttt
     4921 aacaggagat gatagtgtta attatcgcca aattgtaata gaaaaattaa aagaaggtaa
     4981 gattaactat attataactg tagatctttt taacgaaggt attgatattc cagaagtcaa
     5041 tcaagttgta atgttgagac caacagagtc tagtattata ttcattcagc aacttggtag
     5101 aggattaaga aaaagttcaa ataaagagta tgttacagta attgatttta ttggtaatta
     5161 taagacaaat tatttaattc cgattgcgct ttctggggat caatcgcaaa ataaagataa
     5221 ttataagaag tttttaacga ataacgattc gattaatgga gtatctacaa ttaattttga
     5281 agaagttgct aaaaaacaga tttacaattc attggatgca gtatcattaa atcaaaataa
     5341 attaatatta aaagcttatg aagaagttga aaatagattg ggacacatgc cgttactaat
     5401 ggatttcata caacaacatt ctatagatcc aagcgttata ttttctaaat ttagtaatta
     5461 ttacgagttc ttagtgagat ataaaaaaat agatacatta ttgacggaaa atgaatctaa
     5521 aaatctggtt ttcttttcaa ggcaaatagc acctggattg aaaagaatcg atagtttagt
     5581 tttagaagaa cttttaaaaa atgaattaac atatgatgaa ttaaaaaata aaatgttgaa
     5641 cgaagttaag gatataacag aagaggatat agatacttca ttaagaattt tagatttttc
     5701 attttacaac gcaggaattg aaaaaatcta tggaagtccc attatagaac gtaatgaaag
     5761 aatgataaga ctaagcgatg catttactaa tgctctaagt aatcaaacat ttaaaatatt
     5821 tttagaagat cttattgaat tatcaaagta taacaatgaa aaataccaaa aaggtaaaaa
     5881 tggactgatt ttatataata aatattcaag agaagatttt agcaaaatat ttaattggaa
     5941 taaaaatggt tcgtctgtaa tcatgggata catgataaga agccaagaaa tgccaatatt
     6001 tatcacttat gataagcatg aggatataag tgatagcaca aaatatgaag atgaattttt
     6061 aagtcaagat gaattaaaat ggtttactaa atctaataga acgctaaaat caaaagaagt
     6121 tcagaaaatt ttgtcccata gagcaaaagg aattaaaatg tatatttttg tacagaaaaa
     6181 agatgatgat ggtatatatt tttattattt aggaactgct ggatatatag aaggttcaga
     6241 aaagcaagat aaaatgccga atggttcaaa cgtagtaacg atggatttag cactggataa
     6301 agctgtaaga gatgatatat atcgttatct aacaaattaa tgtaaaccga aaaatgaatg
     6361 ttagtaaagt tgtatttaaa catcaatttt aataagtaaa tacattgagt ttcagtgtta
     6421 ttattttaaa tataagcgca atttatctta attaattagt tggttaggcc aataaaa
//
LOCUS       DAHHYJ010000139         6355 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.139, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000139 DAHHYJ010000000
VERSION     DAHHYJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.139"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            142..684
                     /locus_tag="NBK22_002216"
     CDS             142..684
                     /locus_tag="NBK22_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCD2415392.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPYAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNSWKDVEQYFLDNIEK"
     gene            complement(1309..2226)
                     /locus_tag="NBK22_002217"
     CDS             complement(1309..2226)
                     /locus_tag="NBK22_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCD2415393.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ENEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMSHDLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKINNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            2316..2417
                     /locus_tag="NBK22_002218"
     CDS             2316..2417
                     /locus_tag="NBK22_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415394.1"
                     /translation="MVITDIDVYRVGRENIVIAVYIEHIKLLFILIS"
     gene            complement(2495..4000)
                     /locus_tag="NBK22_002219"
     CDS             complement(2495..4000)
                     /locus_tag="NBK22_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCD2415395.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(4341..4841)
                     /gene="queF"
                     /locus_tag="NBK22_002220"
     CDS             complement(4341..4841)
                     /gene="queF"
                     /locus_tag="NBK22_002220"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCD2415396.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(4861..5727)
                     /locus_tag="NBK22_002221"
     CDS             complement(4861..5727)
                     /locus_tag="NBK22_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD2415397.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGRLSSQPLLITRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
BASE COUNT         2249 a          975 c         1017 g         2114 t
ORIGIN      
        1 atttatgtct caagctcaat gcagtgtgaa tgatgaggtg agagtattca gtgtaaaaag
       61 caacaataga tgatattgtt ttgtatcaat tgcttttttg ctatactgaa tcaatactga
      121 tattttgagg agaagattaa aatgacccgt aaatcaatcg cgattgatat ggatgaagta
      181 ttggcagata cattaggaga aatcattgat gctgtcaatt ttagagcgga tttaggtatt
      241 aaaatggaag ctttgaatgg tcaaaaactt aaacatgtta ttcctgaaca tgatggatta
      301 attacagaag tattgagaga accaggcttc ttcagacatc ttaaagtgat gccgtatgca
      361 caagaagttg tgaaaaaatt aactgaacat tatgatgtat atattgctac agcagcaatg
      421 gatgtaccaa catcatttag tgataaatat gaatggttac tagagttctt tccattttta
      481 gatcctcagc attttgtttt ttgtggtaga aaaaacatcg ttaaagctga ttatttaata
      541 gatgacaatc ctagacagct tgaaattttt actggtacac cgattatgtt tacagcagtg
      601 cataatatta atgatgatcg atttgaacgc gtaaatagct ggaaagatgt agaacagtat
      661 tttttagata atattgagaa ataaaaatat atcacttgaa aaatttcatg tagaaaagat
      721 gatggatagg ctataaagta attgtgactg agatgaactt ttatgtctta gacactacaa
      781 cactatattg gcagtagttg actgcggggc cccaacatag agaaattgga ttcccaattt
      841 ctacagacaa tgcaagttgg ggtgggcccc aacataaaga aatacttttt ctttagaaat
      901 tagtatttct tatgcatgag tgtaactcat gcattcatat ttttaagtac acattagctg
      961 tgactaatga taaagaatcg ctacataatc aatcattagt cgttctttat catttccgtc
     1021 ccgctctcaa taaatgttag tctatctcat tattataaat cggatgaatg tgttaatcta
     1081 tggcagatta cacgtcatcc gattttttat agaatttgaa aaagacgcat aaaccactat
     1141 gatttaaaat acaacatcaa tcattttagt ggcatgcgcc aaaattatat gtctgttttt
     1201 gaaacagggt aatagcttaa agctaataaa aacgaatata aggtgcgttg aatcttatga
     1261 ttatactcca aacctaatat aatatcgggt taagatcatt ccggatgctt acaaatcatt
     1321 gacagtaagt aactgaatgg catttggtat aacctcaata tcaataggtg tttctaatga
     1381 aatttcgcca tcaatatcaa ctttcattgc tggatctgtt gtaagtgaaa tctttttacc
     1441 aggtatatgc tcaatacctt gagtaatttc attccaattc atgctatcac gctttttaaa
     1501 aatatcattt aaaatactga aactttgttc attaaaaatg aaagtgttca gttcaccatc
     1561 ttgaggagac aaatcagtca atggtatacg actaccacca atgaatggac catttgctgt
     1621 tagtatcatg gtcgtttcgc cagaatatgt cttatcatct attgataatt gataattaaa
     1681 ttgtgttgga tttagcagtg ttttgacagt tgatccaata taactcaatt taccaaatat
     1741 atcttttgaa ccatcttgta cgttttcagc gttttgaaca atgagaccta agccaacaaa
     1801 gttgagtgca tattgattat ttattttaat tacatcgtat gtaccaactt gtgcagaaat
     1861 catttgttca ctagcttgtt tatgattagg tgctatattt agcgtttttg taaaatcatt
     1921 aaaagtaccg cctggtaaaa tgccaatagg gagttgaagg tcatgtgaca taacaccatt
     1981 tataagttcg ttaaccgtgc catcaccgcc aagaataaat aatatatcta catcttttgc
     2041 atagttttta gttttgattt cttggcaata tttaataatg tcaccttcgt tttcactcaa
     2101 ttgaatagaa agatgcttac aaattgaact taatgctgtt gtaacttccc caataccttg
     2161 attaatattt tttaatccac tgtgttcatg gtaaaagagg acaccatgtg tatatttatt
     2221 ttccatagtt tagcctactt tctaaaaatt ggttcattaa atatatatac ccacttttaa
     2281 ttgttaatac caaaaatatg ttttaaatag agaaaatggt aataactgac attgatgtct
     2341 atagagtggg acgagaaaat atagttatag ctgtctatat cgagcatatt aagcttttat
     2401 ttatactgat atcttgaatt taattaatag aaacctataa aaaaacagta agccatttaa
     2461 atgacttact gttttttaat ttaggccaac aatattaacg aatacctttc attgctttaa
     2521 taattaaagg tgagaatgct aatacaattg ttgtaacaat aattgcaaca acacctagga
     2581 aaataaagta atttgtttga cctagtggtt ctattaactt aactaaagta ccattgattg
     2641 cttgtgcaga agcgttagtt aagtaccaaa tactcatcat ttgggcatta aatgctttag
     2701 gtgctaactt aacagcagca ctattacccg ttggtgataa gcatagctca ccgataacac
     2761 aaataatgta cgataaaata acccagttaa ctgagaagtt tgatgaacct gatgcataac
     2821 ctacaatacc aattagtatg tatgacgcac ctgctaagaa cgtaccaatt gcaaatttta
     2881 ctggcaggct aggttgttta gttccaagct tttgccataa aagtgaaata attggagcta
     2941 gtaataaaat aaataatggg ttaattgatt ggaagatcgc ttcaccaaag tttgttttcc
     3001 aaccaaataa gtttaatttc atatctgaat gttcaattcc atatatgttt aatacattag
     3061 acccttgttc ttgaatagcc cagaacacca ttccaagaat aaataatgga ataaatgctt
     3121 taacacgaga acgttcagta tcagtgacat ctttacttct aataattaaa gtgaagtaaa
     3181 tgattggtaa tgcaatacct aatactaaaa cagtattact aactaagtta aatgataatg
     3241 aattagttaa tgcaccaata acgataatta atacaattgc taagacaaca cttccgataa
     3301 taagaccata ctttttcttt tcagctggtg tcaatgggtt agtaggtttc ataccaacgc
     3361 tacctaagtt tttgcggttg aaaagtacat accatactaa acctaatgcc ataccaactg
     3421 ctgcaatcaa gaatccgccg tggaagtttt taacattaac aaagtgttgc aaaataatag
     3481 gtgataataa tgcacccata ttaactgaca tatagaaaat aacaaaacct gcatccatac
     3541 gtctatcatt ttcaggatat aaacggccaa cgatatttga aatgttaggc ttcattaaac
     3601 ctgaaccaat aatgatgaag aacattgatg tgaataagcc gattaatgca aatggtaagc
     3661 ttaaacaaat atgtccgata ataataaaga ctgcacctaa taaagtagcg cctctagtac
     3721 ctgtaattct gtcagcaatc catccgcctg gtattgatgt catatagatt aatgaaccat
     3781 aaactgacat aattgacata gctgttgttt tatcaattcc aaggccatta tctgttacgg
     3841 caaagtacat gtagaaaatg agtagggcac gcatgccata ataactaaac ctttcccaga
     3901 actctacaaa gaagagtacg cctagtcctc gaggatgccc gaaaaatcct gtttgaggta
     3961 tgtcttgaat ttgatttcca tgggagtttt gttgtgtcat gtatacatcc catcctttct
     4021 tcccctaata caatagttta agtaaatttg attgtgcctt atcaactaaa taccccaaca
     4081 aatcacaaaa aaataaaaat attgagaata ttctgttatt aagcgcgcac aattaattag
     4141 agataatata aaaatatact tattgacgtt aaagtgcaat aggtttctat aaaaatgttg
     4201 tttaattcgt gtactaatat tcttttggtg tactttggta taaaatgtta ttttaatgta
     4261 acctattatg tatgataaat gaaaaacaca ccaataagtt aggaaaatca acttattggt
     4321 gtgtttatta aatgatacat ttaacgatta tctatttttt cgggatataa atcatgattc
     4381 atcaaacgat gctcagccat tttttcatat ttagaatttg gacgtccata gtttgtataa
     4441 ggatcaatag aaattccacc acgtggtgtg aacttgcccc agacttcaat ataatgtggg
     4501 tccataagct ctatcaaatc attcataata atattcatac aatcttcgtg aaaatcaccg
     4561 tgatttctga aactaaataa gtataatttc aaagattttg attcaaccat tttaacattt
     4621 ggaatatatg aaatatagat agttgcaaaa tctggttgcc cagtaattgg acataatgat
     4681 gtaaattctg gacagttgaa ttttacgaaa tagtcacgac cttgatgctt attatcaaac
     4741 gattctaata catcaggacg gtagtcgaaa ttgtaagtat tgtcttgatt tcctaataaa
     4801 gttatatctt gtaattcatc ttgttgacgg ccatgtgcca tataaagcgc tcctttaaat
     4861 ttattttttt attattttgg cgtctcggcg tgctttttca aacatgtaat aacttgcacc
     4921 gataataaca acgtaaccta atgttgcata gaaatctgga gattctccga atagaataaa
     4981 tccaagtatt gctgtgaaaa ttatagatgc atacgtaaaa atagaaatat cttttgcggc
     5041 tgcaaaacta tatgctaaag taacaccaat ttgacccaca gcggcagcta agccagcccc
     5101 taatagataa agtatttgca tctgactcat tggttcataa gtatatgcag tgaaaggtat
     5161 taaaacgatg acagaaaata aggagaagta aaatactata gtatatggtg cttctcttgt
     5221 actaagtgct cgaacacatg tatatgctga tgctgcaaaa atacctgaga ataagccagc
     5281 taatgatgga atcatagatg atgaaaattc aggtttcact attaaaagca tacctaaaat
     5341 agcaattatc attgctgtta tttgatactt ccttactttt tcatgtaaga aaacaatgct
     5401 taataaaatc gtccagaaag gattgagttt cattaatgaa tcggcatcac taagtaccat
     5461 atgatcaatg gcataaatat ttaacaatac accaataagt ccaagtgttg atcgtgttat
     5521 taataagggt tgacttgaaa gtctgccaaa cattggctga tggtatttat atataaaaaa
     5581 taatggaata aacattgcta ctaagtttcg tgctaatgat ttttgaaaaa caggaaggtc
     5641 acctgcaagt ctgaaaaaca ctgacataaa actgaaacca atagccgaaa ttaaaatggc
     5701 aatgatacct tttactttag gattcaattt tatcgcctct tttatataaa attaacgtat
     5761 ttatattagc ataaaacaac atgttgtgca taaatagttg aaatttacta taaaaagact
     5821 ataatagact gtaccgaaca aacgttctgt gtttatttgt cggaataata gggcattaca
     5881 cttttatgaa tgtttgtgtt attacataaa acaaatatca attcagtatc aagctaataa
     5941 gctttttctt gatttctgtt gatacaattg agattgacac agatttaaaa aaatcaagtg
     6001 atatctacta aaaaattttt ttaaatttgt tcaagttttt ctaatttagt attggtgcct
     6061 agttggaacg ttttacgaac attcgattag aaaatggcac tttaaatcat agtgtgtctt
     6121 atgtataatg aaacacataa tatagtgttg gtgaaacgaa aaagacacaa tatcttgtgt
     6181 tttgtatgca aatgctttat ttatgaagaa attacattta aaagtaattt aacacagaaa
     6241 tttaatagtt attatcaatt aatagtcata tttttagaaa atgtactgag caaatggaag
     6301 atatccaatg atgtaaacac tacatatagt gatttttata cattcaaccc atata
//
LOCUS       DAHHYJ010000140         6354 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000140 DAHHYJ010000000
VERSION     DAHHYJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.140"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(253..1131)
                     /locus_tag="NBK22_002222"
     CDS             complement(253..1131)
                     /locus_tag="NBK22_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCD2415398.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYALEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1151..4591)
                     /locus_tag="NBK22_002223"
     CDS             complement(1151..4591)
                     /locus_tag="NBK22_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCD2415399.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHEIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSAKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERTFYVSKFDHPENELEVLSSYLISLDQHRETIEDYTSRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKPEMIEDLYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLIGRQETTYQVATDNTQDNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDMSYSTIEDFINSDLEKLKSHLNKNQ
                     LAFIQAVEKHYKLYVNMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVESKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTRVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIINPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAELQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4895..6232
                     /locus_tag="NBK22_002224"
     CDS             4895..6232
                     /locus_tag="NBK22_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD2415400.1"
                     /translation="MKNTFNPLQRLHFYVAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWTIFMILSGTYLL
                     IKKHLISNKGKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSSHHGNAAMPQTKLDYQISIDKVV
                     EQAQNSGIKKPFSIVYPSDKNGAFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVTIGMGFVSWIKRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYVKDRRAK"
BASE COUNT         1991 a         1021 c          945 g         2397 t
ORIGIN      
        1 actaattaat caaatatgtt ttttagatta ataaagttgt aactgtacta ttttgtagtg
       61 tatggtaatt tatttggatg aaatataatt ctttatactc tattgattaa tcaaatatgt
      121 atctatcaaa attcggctta tttattcact ctgacatatc caaatacgca aaaagactat
      181 ttctaccttg tatcgataga aatagtcttt ttatacttaa ttggtttaat ttttcataaa
      241 atcttatact ttttaaaaat gggatgaaat atattttcct gaaacatgta attcatgttc
      301 gttacaaatt gataaaatat gattaagcgt tgttgcaaat gacattttct caaaaagcgc
      361 ttcactatca attggtactt gtgtgtgaat ttcagccagt cgcttcgata aatatagttc
      421 atctaaattg tcattaatct tattacgttg cccagctgat aatgcatcaa tgttttcaac
      481 cacattttca acgctttgat attgctgaat taacttaata gccgttttct cgccaatacc
      541 tttaacacct gcatatccat ctgctgtatc acccataaac gctttaatat cgattagttg
      601 ttgtggttca agggcatatt cttcgttaaa acgatgtaat gtatatctat tataaatgtt
      661 aaaacctttt ttaattagcc agacttcaac attgtcatta atacattgca gtaaatcttt
      721 gtcgcccgta ataatataga catcgttatc agttgaatat tgttgtgcta atgtacctat
      781 aacatcatcc gcttcatagt ttttaacgcc aatatttaca aagccaaatt gctctgaaat
      841 ttctttaaca taatcaaatt gtggtatcaa ttcttctggt ggtgcagaac gattttgctt
      901 ataaccatca aacatatcat ttctaaaagt tgattgtccc atatcccaac atacagctac
      961 atgtgtaggg cgtatttcat gtattgccga aaagatatga cgcacaaacc cttgtattcc
     1021 atttgtaggt acaccttgtg aattgtacat aaattgttta tgaagacttg tagcgtagaa
     1081 atgtctaaat aatagtgcca taccatctac aagtaatatt ttattaggca tttaaaacgt
     1141 tccttctttc ttaatataaa atagttttca gttgttgatg tttatctttc aattgattga
     1201 taagtgttgt atctaaatca aatgctaata aatcgtttat ttgcgtttga atattagctt
     1261 caaaactttc aaattgcaat tcagcttcta cagccatttg cttaacatat aattcaagtt
     1321 gttgcctcaa taatcccatt tgtggttgta ataatcctaa cgtactttga caaattgatt
     1381 catgtatatc tctttgccca tttggattca aaattttacg ttttgttaat tgtttaggca
     1441 atgcatttag catatcgttg aaatcgatgt gcaataatgg ttgctctata ttagctgatt
     1501 caaagttaaa ctgaggatta ataatgacat gtaaatctgc taattgttga acgattggtg
     1561 caatttgctc agtgagttgc ttattaaagt atttttttat acgttctgta attaaagatt
     1621 gctctaaaaa caatcgttga tgaatttgat ctaagtatac tctcgtagac actttctttt
     1681 cttcattaaa atcactattt tgcgtcattt gagaattaaa cactgattta acatcgtcaa
     1741 gtagttgtaa ttttaaacgt gcatttaaat gataaacttg ttcttcaact tcgttgtctg
     1801 tatgttgtag cgtcgcatca attaattgat gttgtaaacg ctgtttatct ttatagtctg
     1861 ttaacctttg ttggcgacgt gaaatatcag ctttatttgt ttcaaattct gtaatcatct
     1921 ctacataaga acgatccatt tgttgaagct gatgaatcat ttgttgttct aaaattgatt
     1981 tagattcaac atcaacaaat tgttgtatgc tttgttttaa ttgatcaatg cccttatctt
     2041 cagcttgcaa tgcatttcga cttgatacag caaaaatgtc tgattgtaag tgtacttgtc
     2101 ttaatgcatc tgatacatat gtttcaactg cttcaagatc atcttgactt tctgctaaat
     2161 cagcagcatt aattaccatt ttaaatgctt ggttttcatt caactggttc atatctttca
     2221 tgtgttctat aaacgctttg tcattatcag taaatgaatg attaaaataa cttacataca
     2281 atattaagtc tgaagaagtt aaaatttgtt cggtttcatt tgtatgcctt tggttatttg
     2341 agtgtagccc taatgaatca acaattattt taccttttaa ccaatcatgc attaatgcaa
     2401 tgtgtactgt cttaacaaat gttgcatatt catcttctgc actccacttt ttcaattcct
     2461 gttgattaat ggcatgtttt tctccatttt ccaacatatt gacatacaat ttataatgtt
     2521 tttcaactgc ctgaataaaa gcgagttgat ttttatttaa atgtgatttt aacttttcta
     2581 aatctgaatt aataaagtct tctatagtcg aataagacat atcttggtat tcaactactg
     2641 cattaatttc atctaataat tgcgattgtg attttagcgt tatataactc tcgtctccat
     2701 aagatatttc tgtagtagct gctgtagtag gatttggaga actgactaaa atatgctcgc
     2761 ctaataatgc atttatcaaa ctacttttac cagcactaaa tgttccaaat acacctattt
     2821 ttattaattt attatctata cgtgttaatg tttcgtggat atcttgcttt gtacgcttga
     2881 acaaaggcac atccgaaatt atatcaagcg ctttttgaat atctatagac atatttgttg
     2941 tagctgtatt ttgatttagc tgctgattat cacgattatc ctgagtatta tcagtagcca
     3001 cttgatatgt tgtctcttgt cgacctatta atttatctag agattcatct aaatgaatat
     3061 agtagtgacg ataattctta gtcgtcaatg actgacgcag ctcatttaat tctgtataac
     3121 gttgatattc ttttaaatca tcactttctt ctgttggtaa ttcatctgcc tgtacatttt
     3181 ctattatttc tttaaaaatt ggtgttgatt gttgttcaac atatttctta atggctttaa
     3241 ccacttcgtc tgaaaatgta agtacataag tattgctgat tgatgtttgt ggttgatata
     3301 aatcttcaat catttccggc ttaacgtcat aatgctgatt taatacttta tctgaagctt
     3361 cttttttatt gataaaacgc gttacaaatg acatatcttc tcgcattggt tgacgaattt
     3421 gttgattaac atgttcttgt aacgcatctg tcgctgttaa taatcgctgt tgttgaattt
     3481 gtagcttttt cttcttttta ttaaaaaacc caccaacatt aaagtcttga gacatacttt
     3541 ccaaataact tcgtaacatt tctctcatat tatgcggcat aatgtatgca ttttctaaaa
     3601 tatttttacg cttattcttt aaaaatgcca ttaattcatc tggattattt aaaagttgtg
     3661 cctcttcact aattgcttga tgttgttgac tatttaaaaa tgcttgttca aactccgctt
     3721 cttcgatacc taaatcttct agtacttcct gaatttcaga ctgaatgtaa tctagctgag
     3781 cttcggtaat gtattcaacc gttcttgatg tataatcctc tattgtctct ctatgttgat
     3841 ctaatgaaat tagataactt gataatactt caagttcatt ttcagggtga tcaaatttag
     3901 atacataaaa ggtgcgttct aatttaatac cccaatctgc aattgatttt tcaactcgag
     3961 atttaaacgt agagaatgac aattcatcgt cttgatgctt gtcaatttga ttaatgataa
     4021 acacaacagg tattccaaca tcatttatat gcttcataaa cttaaagtta agttcagatt
     4081 gaacgtggtt atagtcaacc gtataaaata tcatattact tgtatacata tattgttctg
     4141 ttattgactg atgtgatgca acatttgaat caacacctgg tgtatcttgc aacgtaaacc
     4201 cattttcaaa tttagctgat tgaaaattaa tttctacaga ttcaacgtcg acattttggc
     4261 gattcatttc ccttacttca tcataattag ataatttggc atacgtttga ttcggcaaat
     4321 tagcaataat ttcgtgattg tctgaaactg acacaatagc agtattactt gtcgttggta
     4381 caggtgaact tggtaagata tcttgttcaa ttaataaatt tatcagtgtc gatttacctg
     4441 cagaaaaatg tccaacgaac gaacatgtat attgctgcaa atatactttc ttaattactt
     4501 ggttaattgt atgtaaaagt gcttcatttc gcgacttttc aacttctttt tttaatttat
     4561 ataaaagatc taattgttct ttattaatca tgttactttc ccctttggtt cgttcacata
     4621 gaaatgcttg cttgttaaaa cgaattcata caatatcgat gtccatttaa acatagatcg
     4681 ttaaatggct cgcattgtat tatgtttaaa ttttatcata gctgcttgat aaaacatacg
     4741 aataatcaat tttcttcaaa cattccaact taaaagttca tttttctaaa aatcacaaat
     4801 catcgttaaa tttttctaga aaataaatat tgtcacagtt tgttcacaca cgtcacagaa
     4861 ttgacaccgt ataatagcca attgggaggg aaatatgaaa aatacattta atccattaca
     4921 aagattacat ttctatgtag caatttttat tgctccactg ttaatcactt taaccatttc
     4981 gggcattggt tacttgttct ttccagaagt tgaaaataat atttataaga atgagttttt
     5041 tggtgacagt gatgtaaaaa cgcatcaaac attaaatgat gcagtacatc aagttgaaca
     5101 acaatatgaa ggattctttg taagtaaagt tagcatactt gatgaaccat ataacaaacg
     5161 aattacactg agtgatatgg caggaaatca acgttacgtc tttctagatc ataacaatca
     5221 atttgttgca gatcaaaatg cgaaacatac gtattctaat gtgatgcgaa gtatacatag
     5281 ttctttgttt actgaaaata ctattattaa ttatttagta gagttaaccg catgttggac
     5341 gatattcatg attttatctg gtacttattt actcattaag aagcatttaa tttctaacaa
     5401 aggtaaggca cttcgttggc aaaagtggca cgcaatgatt ggagttatca ttgcaattcc
     5461 agtatttgta ttagttttaa ctggattgcc atggtctggt tttatgggca gtaaaattgc
     5521 cggtatgatg gacacaaacg gtgaccttgg tcaaggtgaa ttagcgatta atccacctaa
     5581 atcagatttg aacgaattac cttgggctac acgtaaaaat aaacagccag cttcatccga
     5641 aaaaggttca agtagtcatc atggtaatgc agcaatgcct caaaccaaat tagattatca
     5701 aatatctatt gataaggtcg ttgaacaggc gcaaaattct ggtattaaaa agccgttttc
     5761 aatcgtatat ccaagtgata aaaatggtgc ctttattgta tctaatacta gtaattcagg
     5821 tgttactggg ctagatgtat caccttacaa ggaacaaaca ctttatttcg atcaatatag
     5881 cggtaaaaag ctaggtacga ttaaatatga tgactacggt attattgcta aatggtttac
     5941 atggggcatt ccgcttcacg aaggtcattt attcggcatt ttaaataaaa tcattaattt
     6001 atttgtatgt atcgctttat tagtaaccat tggcatggga tttgtctctt ggataaagcg
     6061 tacaaaaaat actgcagtaa aagtaccaca tcgcgtaaaa aaaccagcat ctatatcact
     6121 catcatatgt ttaattgtat taggactatt aatgccatta tttggattat cacttatact
     6181 agtattcata attgaattaa tattatatgt taaagaccgt cgtgctaaat aatcatacac
     6241 ttaaagtttt gaactgacga aatttacaaa atgattctcg actctctaat tacttaaaac
     6301 gggttctata ataaatcgta ctgatggagc ttgggagata aatcaatgtc ctag
//
LOCUS       DAHHYJ010000141         6349 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000141 DAHHYJ010000000
VERSION     DAHHYJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.141"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            109..1029
                     /gene="rnz"
                     /locus_tag="NBK22_002225"
     CDS             109..1029
                     /gene="rnz"
                     /locus_tag="NBK22_002225"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCD2415401.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     CVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKV"
     gene            complement(1132..1947)
                     /gene="proC"
                     /locus_tag="NBK22_002226"
     CDS             complement(1132..1947)
                     /gene="proC"
                     /locus_tag="NBK22_002226"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCD2415402.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNVEDQ"
     gene            2089..2844
                     /locus_tag="NBK22_002227"
     CDS             2089..2844
                     /locus_tag="NBK22_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2415403.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDMKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNLHIMTVNVGPIDTPFHQKADPSMKYAKKMGKIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            2860..3108
                     /locus_tag="NBK22_002228"
     CDS             2860..3108
                     /locus_tag="NBK22_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415404.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDSTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(3324..4232)
                     /locus_tag="NBK22_002229"
     CDS             complement(3324..4232)
                     /locus_tag="NBK22_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD2415405.1"
                     /translation="MQKNILKSGIALSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIVIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHTNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            4314..4856
                     /locus_tag="NBK22_002230"
     CDS             4314..4856
                     /locus_tag="NBK22_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD2415406.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCALTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDENRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            4961..5410
                     /locus_tag="NBK22_002231"
     CDS             4961..5410
                     /locus_tag="NBK22_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD2415407.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            5459..6346
                     /gene="xerD"
                     /locus_tag="NBK22_002232"
     CDS             5459..6346
                     /gene="xerD"
                     /locus_tag="NBK22_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCD2415408.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTITEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
BASE COUNT         2190 a          947 c         1142 g         2070 t
ORIGIN      
        1 attaactagc aaagacgaat ttttaacaga ttttgattca agtataaatt aaaactaaat
       61 tgatacaaat tttatgataa aatgaattga agaaaaggag gggcatatat ggaagttaca
      121 ttttttggaa cgagtgcagg tttgcctaca aaagagagaa atacacaagc aatcgcctta
      181 aatttagaac catattccaa ttccatatgg cttttcgacg ttggtgaagg tacacagcac
      241 caaattttac atcatgcaat taaattagga aaagtgacac atatatttat tactcatatg
      301 catggcgatc atatttttgg tttgccagga ttactttcta gtcgttcttt tcagggcggt
      361 gaacagaagc cgcttacatt ggttggacca aaaggaatta aagcatatgt ggaaatgtct
      421 atgaatttat cagagtcaca tttaaactat ccaattacat atattgaaat cgatgatcat
      481 ctgacatatc atcatgatgg ttttactgta gaggctcatt tacttaatca tggtattcca
      541 tcatatggat atagagtaat ggcacctgaa actacaggta caatcaatgt tgaagcgtta
      601 aaaaacattg gtcttgaacc aggtcctaaa tatcaagaag taaaatctca tgatacattt
      661 gaacataatg gtcaagttta tcaatcaaaa gattttagag gtgagtccaa acagggtcca
      721 gttgtagcaa tttttggtga tacaaaacca tgttctaatg aatgtgtgat ttctagagat
      781 gctgatgtga tggtacatga agcgacctat atagatggtg aaaagcattt agccaacaat
      841 tatcatcata gccatattga agacgtattt gcattgataa aagaagccaa tgttaaacgt
      901 acgttgatta cacatttgag caatcgctat aatacagaag atataaatga aatttatcaa
      961 acattgatac aaaatgaaga tacacctaat tttaattttg taaaagattt tgatagtttt
     1021 aaagtctaat gacaaagtaa taaacaatgc ttgccaaata gcaataattt ataaaaataa
     1081 cccgtccaac tatgtatgcg atgatgcata catgtgttgg cgggtttgtt tttattggtc
     1141 ttctacatta gaaagttcaa tactacggtc gacggcagca tttagacaat cttcgaaaat
     1201 agatactaaa tcatattgtg acaatgtatc aaggccagct tgtgtcgtac cacctttaga
     1261 ggtaatattt tttcttaatt gagccatgct caaatctgaa cgttcaatca tcttacttgt
     1321 acctataata aggttgcgta tagattcttc aacttgttct ttttctagac caagtttcgt
     1381 accagcttta acgtattgct cgaatacatg atataaaaat gctgggccgc ttccggtgat
     1441 agctgttact tgatgtaaat gatcttctga tacttcaatt acagaaccaa atgctttaac
     1501 taaatcgtta atttcatctt tagatttagg gtcaaagttg tttgaaaaac taatgccagt
     1561 aacagagtgt ccgacttgcg catttgtgtt tggcataatt ctagcaactg gattttggca
     1621 ttctaattgt tgtttaatat aatcaatcgg aatacctgcc ataattgaaa tgaagcaatt
     1681 gtcttttgtg atatgtggtt tgatgcgtgt tgctagagca tcaaagtcat gcggtttggt
     1741 acctaaaaaa acataatctg catcttttaa taatgtcgca tcatcataac tatagttaac
     1801 acctagtttt tcagcgaatg cttttaaagc ttgttcatta gatttatttg ttaaatatat
     1861 atcattggca tctaagttgc ttgagttaat aattcctgta aatatagctt gtgccatatt
     1921 accagctcca taaaatacga gtttcattaa cgtcacatcc ctttagtttt tgatttgtat
     1981 gttatatttt cttattattg tatatatatt aggaaagata atcaacttgt ctcaattatg
     2041 taaagttgat aaattgcatt ctatataatt gaaaaggaag gttaattgat gaaaggacaa
     2101 cattttatag tgactggtgg tacgagtggt ttaggtttat cgattgtccg aaagttactt
     2161 gaaaataaag tgcatgtaac gttgttggtg cgtgatgttg ataaagcaac tagaatcttt
     2221 gaacaggaat tgggaaaaac tataaatgtt attccttgtg acttaaatga tatgaagtcg
     2281 atacaggcac tgcaatttga agacaataca tcattcgatg gattcattta tagtgcaggt
     2341 ttaggttatt tcaaatctat ttcagaccat agtttttcag aaatgattga aacgtatcag
     2401 cttaatttaa ttagttttaa tgttttatat actgtgttga gaccatattt aacatctaac
     2461 gcacatattg taggtatttc aagtcaagct gcattttcca ctcaagcaaa tgctgcacat
     2521 tatggggcat cgaaagcagg gttttatgca ttaatgaatg ctttaagact agagtcacca
     2581 aacttacata tcatgacagt taatgttggc ccgattgata caccttttca tcaaaaagcc
     2641 gacccatcaa tgaagtatgc taaaaagatg gggaaaatta tgcttgatgc taaccagctt
     2701 gcagaagata tcatttatgg cattaaaaca aaacaattag aaattaatcg gcctaaatgg
     2761 atgcaccatg cgttgaaaat gtatcaaatt gcgcctagat tttttgaacg atgttttcct
     2821 aaattattta aaaataaagc ataaataggt gggatggcaa tgaactgtca atcggagagt
     2881 gtggtaagac tttgtgtacg atatgcggaa caactatcag tgtttgaaga atttacagtg
     2941 ctggatattt taggcgatat ttctgtagac caaatttcag acagtacatt atattatact
     3001 tgtgaaaaat tcaaactact tgtgttgcaa ggaaatgtgc tgggtgtaca aataataact
     3061 aataatgatg aatcaacatg cgaagtgaaa tatagaaaga tgttttaata aaattattaa
     3121 aagatgaagt ggcagaagtt tgcttaaggc actgccgcag ggcgaaatta ctaaatactg
     3181 atgcgtgagg acgactcttt aatttctatc ataagtgagt aatcttaatc ataaattata
     3241 gcgaattgta cataaacaac agcagtaagg catttttata atgaaacgcc tcatgccgct
     3301 gtttttatta ctgaaaatga cttctacact aaatggttgg tatattccaa atcctttaca
     3361 cgagctcttg ctgttttaat ctgatctaaa ctaactttag tatgatagtt ttcaatattt
     3421 tcttttaatt ggtcgacgct acttgcacct acaatgatgg aaccaagcac gtcatgtgat
     3481 gttaaataac taaatgtcaa tgcagataaa ttactttcaa tttctttaat agaggctatt
     3541 gtttcaccca attcatcatg agaataatca aaaataccat ctttaaattt attgtcgagc
     3601 acattaacac tgtttgaagt taataatcct ttagacacag gtcctcttgc caatacttta
     3661 acatcattgg tgtggattgc atctaataat gattctggac gattatcaat caaattgaat
     3721 tgagacatta tcgtttcgat ttgactatgt tttaaataat aatcaattac atttgggcga
     3781 atagaagaaa taccgtaagc acgtataatt ccttcttgtt tcaattcatc aaatgcgctt
     3841 attgtttcgt ctaatgggtc atcaatggtt ccgccatgaa gttgatataa atcgatatga
     3901 tcgataccta aacgctttag tgaaccttta actgcctctt taatatagga tttactcgga
     3961 tcccatgttg tactgccatc ttttgttaaa cgattgccta cttttgtacc aatgacaata
     4021 tcatcgcgtt gttgatattt aagaagtgcc ttaccaacaa ctgattcatt tataccttta
     4081 tcgtacatgt ctgctgtatc aaaataagtg ataccatttt caacagcaca atctataatt
     4141 tgttcggctt tttttaaatc tgtacctaaa ctcatgcaac ctagacctaa ttcagataat
     4201 gcaataccac tttttaatat atttttttgc atgtctgttc tcctttcgat acactttagc
     4261 taacaagcat agtgtatcaa tatataatat gaatttaaag gaacgtgatt ataatggatt
     4321 taaatgaaaa aacaattgat cgaacagtta tttataatgg caaaattgta gatgtagaaa
     4381 ttcatacagt gacattacca aacggtgaaa cgtcaacaag agaattagtt tatcataatg
     4441 gtgcagttgc tgtttgtgca ttgacaccta aaaaagaagt cgtattagtg aaacagtatc
     4501 gtaaaccagt agaaaaacca ttgctagaaa ttccagctgg taaattagaa gatgatgaaa
     4561 atagagtcga agcggctaag cgtgaattag aagaagaaac aggatatatt gccaaagagt
     4621 taacacatgt tgtagatatg tatggttcac caggtttttg cgatgaacaa ttatcaattt
     4681 attttactga taatttagaa gaaggtacag ttcacttaga tgaagatgaa tttgtcgaag
     4741 tcattaaagt tccgattgag aatgttaaat cgatgctaat gaataaagaa atagaagatg
     4801 cgaagactat cattgcattg caacacctat tattaaatta taatcattct aaataacaaa
     4861 tgagaaaagc ttgcatttta ttgagaaaac tgttagtttt aattgtgaag tttgaaataa
     4921 tttgtaatga ttttaattat tagtagggga gtggacatcg ttggaagaac gattaaatcg
     4981 cgttaagcaa caattacaac aatcatcata taagctaacg ccacaacgcg aagctactgt
     5041 tagagttcta attgaaaatg aaaaagatca tctaagtgct gaagacgtat atctgaaagt
     5101 aaaagacaaa gcgcctgaaa ttggcttggc gacagtatac agaacgttag agttgttagc
     5161 tgaactaaaa gttgtcgaca aaattaactt tggtgatggc gtcgctcgtt ttgatttaag
     5221 aaaagaaggc gcaaaacatt tccaccatca tttagtatgt atggaatgtg gtcgtgtaga
     5281 tgaaatcgat gaagatttgt taccagaagt tgaaaatcga gttgaaaatg agttcaattt
     5341 taaaatttta gatcatcgtt taactttcca tggtgtgtgt gaaacgtgcc aagctaaagg
     5401 taaaggatag taaattgcgt aggttaaatt aaccttcgct ttttttagag gtgtggttat
     5461 ggaaacaatt attgaagaat atttgcgttt tatacaaatt gaaaaaggac taagttctaa
     5521 cacaattggt gcttatagac gtgatttgaa aaagtatcaa gattatatga ctgaacatca
     5581 tatctcgcat attgatttta tagatcgaca attaattcaa gagtgtttgg ggcatttaat
     5641 agaccaaggg caatctgcta aatctattgc gcgatttatt tcaacaatcc gtagttttca
     5701 tcaatttgct ataagagaaa aatatgcggc gaaagatcca acggtattat tagattcacc
     5761 aaaatatgac aaaaaattgc ctgacgtttt aaatgttgac gaagtgttgg ctttattaga
     5821 aacgccagat ttaaataaaa ttaatggtta tcgtgatcgt acgatgttag aacttctgta
     5881 cgcaacggga atgcgtgtat ctgaattgat acatttagag ttagagaacg tgaacttaat
     5941 aatgggattt gtacgcgtat ttggtaaagg cgataaagaa agaattgtac cattaggcga
     6001 cgcagtcatt gagtacttaa ctacttatat tgaaacaatt agaccgcaac ttttaaaaaa
     6061 gactattacc gaagtcttat ttttaaatat gcatggtaaa cctttatcgc gacaagcaat
     6121 atggaaaatg attaaacaaa atggtgtgaa ggcaaacatt aaaaagacgt taacgccaca
     6181 tacgttacgc cactcttttg cgacacattt attagaaaat ggcgcagatt taagagcggt
     6241 gcaagagatg ttaggtcact ctgacatatc tactacgcaa ctctatacac atgtttcaaa
     6301 atctcaaatt agaaaaatgt ataaccaatt tcatcctaga gcataaagt
//
LOCUS       DAHHYJ010000142         6337 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000142 DAHHYJ010000000
VERSION     DAHHYJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.142"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..1148)
                     /locus_tag="NBK22_002233"
     CDS             complement(<1..1148)
                     /locus_tag="NBK22_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCD2415409.1"
                     /translation="MTTTFIISYIILALIIVGVINLFLIRSRKKGKRQQKEQQFTTRQ
                     SNQSKFKASDLDKTTDQSTQRMTHEELRVDNQDDHSQVSLNGYTKGSEKDQEAFTNNN
                     GEEAVAAKNPESEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTLLN
                     VALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFTIG
                     SLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMILA
                     PAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILIGFVWFKLYQYTTNPKADIPGIIFS
                     TIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKSP"
     gene            complement(1161..1808)
                     /locus_tag="NBK22_002234"
     CDS             complement(1161..1808)
                     /locus_tag="NBK22_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCD2415410.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYVTTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMDLKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASIKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLESEPSKQVVPGMNAEVKIHKN"
     gene            1929..2480
                     /locus_tag="NBK22_002235"
     CDS             1929..2480
                     /locus_tag="NBK22_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD2415411.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKHNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQN"
     gene            complement(2710..3918)
                     /locus_tag="NBK22_002236"
     CDS             complement(2710..3918)
                     /locus_tag="NBK22_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCD2415412.1"
                     /translation="MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGKGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMRVFKTNR"
     gene            complement(4028..4155)
                     /locus_tag="NBK22_002237"
                     /pseudo
     CDS             complement(4028..4155)
                     /locus_tag="NBK22_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4186..5568)
                     /locus_tag="NBK22_002238"
     CDS             complement(4186..5568)
                     /locus_tag="NBK22_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCD2415413.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGYLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSSKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            complement(5808..6263)
                     /locus_tag="NBK22_002239"
     CDS             complement(5808..6263)
                     /locus_tag="NBK22_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2415414.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
BASE COUNT         2087 a         1137 c          824 g         2289 t
ORIGIN      
        1 attggtgatt tcattcttaa ttctcttatt acaaatagaa taataaagat aataccaatc
       61 gcaaacattg tttctatctc aacagaaccc catcctttgt tccccgcttc tgaaaagccg
      121 tataacaaag caccaaaacc aatcgtacta aaaatgatac ctgggatatc agctttaggg
      181 tttgttgtat attgatataa cttaaaccat acaaaaccaa ttaaaatagc gataatcccg
      241 ataatgaaca taccgtaaaa catcacattc caatggtaat tttgtacaat ataacctgat
      301 aatgttggac caattgcagg tgctaaaatc attgcgatac ccattgtacc catggcagca
      361 ccacgttttt caggtggata aattgtaata ataacaattg aacctaatgg cattagtaca
      421 cctgcaccaa tggcttgtaa tacacgtcca accatcatga ttgggaaatt cattgaaatc
      481 gcacagatta atgaaccaat tgtaaagagt actaacgcaa ctaaaaataa ttttcgatat
      541 gaatatttat taaatagata cgccgtaatt ggtattaaaa taccgtttac taacatgaat
      601 cccgtcatca accattgccc tgttgacgca gaaatattaa attccgtatt aatttttggc
      661 aaagcaacat ttaataatgt ttggtttaaa atcgcaataa acataccgaa taataatgcc
      721 gctaatattt taccgcgtga aacaccttca ccaaaaataa agtttttatg ttcttttttt
      781 attttttcat tcactttata ttcttctgat tcaggatttt tagcagcaac tgcttcctca
      841 ccattattat tagtgaatgc ttcttgatct ttctcagacc cctttgtgta accatttaga
      901 ctaacttggc tatgatcatc ttgattgtca acacgcaact cttcatgcgt catacgttgt
      961 gtcgattgat cagttgtttt gtctaaatca ctagctttaa atttagattg atttgattga
     1021 cgtgtcgtaa attgttgttc cttttgttgg cgtttgcctt tttttcttga tcttattaaa
     1081 aataaattga taaccccaac aataatgagc gctaaaataa tgtagctaat aatgaaggtc
     1141 gtagtcattt aatgaccccc ttaattttta tgaattttta cttcagcgtt cattccagga
     1201 acaacttgtt tagacggttc tgattctaga gtaattttaa caggtattac ttgagaaact
     1261 ttagtgtagt taccatcact atttgatgat ggcattaatg aaaagcttgc agcagttgct
     1321 tttccaatac tatcaacttt acctttaata gaagcttttt gaccgtcaat agtcacatca
     1381 acatctttac ctacttcaac atctttaata tctttttcgt caatatttgc tgttacatat
     1441 aaatcatcta aattgtatgc ataagcgatt gggttaccag cttgcaccat tgaaccttcc
     1501 ataccatcta atttggcaat tgtacctttt tgaggcattt taagatccat atctttcgtt
     1561 tcgccatctt gaccttgtac agtaacagtt gctactttgt cacctttatc gagtttgtca
     1621 ccttgtttaa cattaagtga tttaatttgt ccagatgcag gacttgctat tttaatttga
     1681 tcgccattta cttttgcatt atcagttgtt acatagcttg ttgttttatt ccagaaataa
     1741 aagccagcaa tcccaattgc taacagtaca acaaccgtaa tgacattaat taatatcatc
     1801 tttttcatga aacatttcct cctatttgct tttaaaattc actttattta ttatagagac
     1861 atgataacta aaaaaacacc aacaattttt ataagagtgt tggttaaaat tcacaaaggt
     1921 gtaacaatat gaaacgacaa gccaaaatag aaattcaaaa cgcacttgtt gatttgatgg
     1981 ctgaatatcc ttttcaagaa atatccacta aaatgatttg cgcgtattgt aatattaatc
     2041 gttctacttt ttatgattat tacaaagata aatttgattt attggatacc atcaactcaa
     2101 agcataaaga aaaattccaa tttcttctaa gcgcgctaca tcataatttc gaaaatatta
     2161 aacaggataa gcttaaactt tataaattct tcattattat tgctaagtat atcaagcaca
     2221 atgagcagtt tttcaaagac attttagtta cctatccaat gaaaacatta ttcatagact
     2281 atataaattt agcgagagat tactatcaac aaattatgaa tgactatagt actacagtaa
     2341 ccaataaaca gctttatgtt acatatatca taggcggtca agctggggtg tttattaatt
     2401 ggctttcgaa tggttgtaat gaatcacctg aagaagttgc tgacatttta ttggctaata
     2461 cgattaaact tcaaaactaa taatacggtt tcattctaat aaagaaatgc tataaaacaa
     2521 ctagataaag ttaaaccaaa tggatagata atagtacata tgttacggtg ctatcaatat
     2581 aaaaatgccg agatttccac ataataatct cggcattttt atttttgatg ctaaattcat
     2641 aggtctatga atagctcaag ttttgttttt taatttttca attcaatata atagaagaaa
     2701 ctatgtttat catcgatttg ttttaaatac cctcatatta taaatctgta aaataattaa
     2761 tataactgct gtagctataa taataattat gtaaggtata ggattatctt caccttttac
     2821 ccccactaat ggcgatgcaa caccaccaaa taaaaactgc actaaaccta ataaacttga
     2881 tgaactccct ttaccactac tactttcatc catagctatt gtaaaaccaa gtgtcgcaac
     2941 acctgtcact ggtgctatta aaatgataaa tcctatcgct aaaatccaaa agttccaatg
     3001 attaagcaat acaatcgtca ctaaaattac accgataatc tgaatcatcg tcatgcctct
     3061 catcaatttt tgtgagtcta taaaatcaac aaggtaccca gtaagttgac ttgatataat
     3121 gagcgtaata ccaattcctg caaacatcca actaaattgt attgcagtca tgccataaat
     3181 tttttgaata ataaacggag acgcagaaat ataagtaaat aatattacaa atgtcatacc
     3241 ttgaatgagc attggtaata caaaacgtgg cgtcttcaat aatattttga agtttttaaa
     3301 cattgtcttt aatccactac ttgactcacg atttgtcact gttaatgatt caggtacttt
     3361 taataaagaa cctatgacca tgacgaagcc aaagatagtc agaatgacaa agaccatacg
     3421 ccagacagaa taatttaaaa ttatgccccc tattgttggc gcaacaactg gtgcaatacc
     3481 attaacaagc atcaataatg ccataaactt agttagttca ttaccactat acatatcact
     3541 tgctatagct cttgaaataa ctgctgctgc gccacctgtc actccttgaa gaaatcttaa
     3601 tgcaaccatc agccaaatat tatgtacaaa aacaatacct aaacttgcta atgtaaaaat
     3661 aatcatagct ataataagcg gctttctgcg cccagttgaa tctgaaattg gaccagcaaa
     3721 caaattacca aacgctaaac caatcataaa catcgataat gttaattgtg cgttagacgt
     3781 tgttgtttga aaatcatgtc taatatctgg taatccaggc aaaaacatat ctatcgataa
     3841 tgctccaatg gcagtcaaag atcctaaaat aataataaat atcggtgaac gcttgttaaa
     3901 atgcgttaaa tcattcatat gactatctcc tttatataat gattagttta taactatcac
     3961 gaacagaaac taaatttaaa tatgctcttt tatatattag cattagtcac aagtttcatg
     4021 aacatcatta caacttataa aaggtataaa atgatataaa cctcagcact tcataaattt
     4081 ttccacaaaa acaatccctt caaagtaatt cacactaatt gtaaatactt tgaaaggatg
     4141 ttgtttgtat tccatgacac atgcatctta tttaagtttt atactctatt tttctgatgt
     4201 cttgattaat tgcaagcggt cttgcgagcc ttgttcaagc tcatattctt tatctacttg
     4261 cttaccgttt tcatttatta atcttacggt tgcggtaatc ttatcaccat gtttttcagc
     4321 atctataatt tgtggtgaac taatcatcat taaacttcct ttagatacac gcttctttac
     4381 atcatcataa aaagatgatc ctttttttat atatgatgat acaaaatcaa aatccgactg
     4441 attaaacgca gcattattcg ctaaagaata tccagcaaag aattctatca atttgttctt
     4501 caagctgttt tcttcttttt ctttcttttc aacatagtct tcgatatctt cactgtcaaa
     4561 attcaaagtt atttctgtat tgtattttaa atcgctagct ttaatcgtct tcgtctgtgt
     4621 tgtaaatgtt ttatctttcg ctttacctga agctgaaatg gtaatatctt tattttgtgg
     4681 atatggacca tacgttttgg aacttgaaaa tgccatttca cggtcattta tagttacttt
     4741 tttagaacta tcatttaatt ttgtatcgcc ttttaaagtt acagttatat ttgcttcttc
     4801 aaaatcttca gtaacatcta ccgtttcaga attactttgt ctaaaatcaa attttaaata
     4861 gcctgcaaaa tcaccgttct cagttgattt catagctgga attctatatg tacccggtat
     4921 aaaattacct attggtgtca ctttatttgc ttcagctata accatctttt tcttaccacc
     4981 agatttaaac tcatattttg ttttttcttt cggtttaaca attggttgct tggtaggtgc
     5041 agtaaagctc atattatcga aaaagatata acgcgtgcca tttttactta cacgtaatat
     5101 attttgacca gatctggttt gaatataaga agctacgctg gtcttactct tattcaattt
     5161 atgtaccgta tttttaaggt cgctgacaaa ttgcttaagc ccaacttcat ctttgatata
     5221 gttaatgtat actttcgctt cttcagaatc tactttatta tctttagtac ttaataatgt
     5281 tgctactttt tgtttatcat tattctcaat cgcattcact aatattttag tttgcgcttc
     5341 aggtgaattg aagtttctta gtaaaaagaa caatataata attaagatta aaatgaaaaa
     5401 accaattgcc caaggcacca tttttctcat cttgatgtta ttagtttgta tatcttcatc
     5461 ggttgacttt ctatataatg cttgacgact atcaaattta tgtccacatt gtgtacatat
     5521 ctgtacatca tcttgtgctt gttgtccgca cttcgggcaa gatttcatac tttcacctca
     5581 attctgttcc tcttcaattg ttatatgtat atcacaaaaa ttatttcatt attttaccat
     5641 tattatttta caaatattat cccaagaata caattcaaga aatgattaac tttactttag
     5701 aattgtacct tattacatta tttatgaaag tactctctca attataacta aaaagaaggt
     5761 gttcaataca tacatattta gtcttatatt tgaacacctt caaaaaccta tttatgattg
     5821 ctatatgttt taatgcgatg attgatgact tctaatactt gccttacatt ttcaatatct
     5881 tcagaattta aatcattagt aatatcttgc gcaatatctg tcataatcgc attacgttct
     5941 ttcaaatatg ctctaccttt gtcagttaag gttatgaatt tcaaacgttg atcaatattt
     6001 aaatttggtt tatctaactt aactaattta gcatcgatta actttttaat tcgtcggctt
     6061 actgcggcct tatttacacc ttgtctttgc gtgatttcac taattgtcaa aggttcttta
     6121 tttaacatac ctaatacgtt agactgctct aatgaaattt catattcatt ttgtaaatct
     6181 ttcaacattt ttgcagttaa tgcgttaacg ttatttataa actgctctaa aaattgaata
     6241 tggtcttgta aatgttttac cataattatt tctccctaat tactattttt ataaagtatc
     6301 ctcagcaatc attgtatcgc acatctaaaa aacacaa
//
LOCUS       DAHHYJ010000143         6241 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000143 DAHHYJ010000000
VERSION     DAHHYJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.143"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            58..774
                     /locus_tag="NBK22_002240"
     CDS             58..774
                     /locus_tag="NBK22_002240"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCD2415415.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            767..1792
                     /locus_tag="NBK22_002241"
     CDS             767..1792
                     /locus_tag="NBK22_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD2415416.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            1814..3502
                     /locus_tag="NBK22_002242"
     CDS             1814..3502
                     /locus_tag="NBK22_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD2415417.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDAYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            3535..5256
                     /gene="tarS"
                     /locus_tag="NBK22_002243"
     CDS             3535..5256
                     /gene="tarS"
                     /locus_tag="NBK22_002243"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD2415418.1"
                     /translation="MMKFSVIVPTYNSEKYMTELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLSVNANKQYRFNIMLEDIKAYLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISLKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMIMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            5400..6074
                     /gene="scdA"
                     /locus_tag="NBK22_002244"
     CDS             5400..6074
                     /gene="scdA"
                     /locus_tag="NBK22_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCD2415419.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAASLEK
                     KNVDLNELLQRLNDVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKETYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
BASE COUNT         2282 a          845 c         1158 g         1956 t
ORIGIN      
        1 aatgcaacga aatttaaata attaaatata gatatagttg aatggaggaa gtattttatg
       61 aaatacgctg gtattctagc tggaggtata ggctcaagaa tgggtaacgt acctttacct
      121 aaacaatttt tagatttaga caacaaaccg attttaatcc atacattaga aaaatttatt
      181 ttaattaatg attttgaaaa aattattatc gcgacgccac aacaatggat gacgcatacg
      241 aaagatacac ttagaaaatt caaaatttct gatgaaagaa ttgaagtcat tcaaggtggt
      301 agcgatcgta acgatacaat tatgaatatc gttaaacata ttgaatcaac aaatggtatt
      361 aacgatgacg atgtcattgt gacacatgat gcagttagac catttttaac gcatcgtatt
      421 attaaagaaa atattcaagc tgctttagag tacggtgcag tagatacagt gattgatgct
      481 atagatacga ttgttacatc taaagataat caaacgattg atgcaattcc agtgcgtaat
      541 gaaatgtacc aaggtcaaac acctcaatcg tttaatatta atttattaaa agaaagctat
      601 gcacagttga gtgatgagca aaagagtatt ttatctgatg cttgtaagat tattgtagaa
      661 acaaacaaac cggttcgact tgtaaaaggt gagttatata acattaaagt aacaacacct
      721 tacgatttaa aagtagcgaa tgctattatt cgaggtggta ttgccgatga ttaatcaagt
      781 atatcaatta gttgcaccta gacaatttga agttacgtat aacaacgtag atatttacag
      841 tgactatgtc attgtacgtc ctttatatat gtcaatttgt gctgccgatc aaagatatta
      901 tactggtagc cgtgatgaga atgtcttatc tcagaaattg ccaatgtctt taattcatga
      961 aggtgttggt gaggtcgtat ttgacagtaa aggtgtgttt aataaaggta caaaagtagt
     1021 tatggtaccg aatacgccga cagaaaaaga cgatgtcatt gctgaaaact atttaaaatc
     1081 gagctacttc agatcaagtg gacatgatgg gtttatgcaa gattttgtgt tgctaaatca
     1141 tgatagagct gtaccactac ctgatgatat tgatttaagt attatttcat atacagagct
     1201 tgtaacagta agtttgcatg ctattcgtcg ttttgaaaag aaatctattt caaataaaaa
     1261 tacatttggt atttggggtg atggtaactt aggttacatt acagccattt tattacgtaa
     1321 attatatcca gagtctaaaa tatatgtctt tggtaaaaca gattataaat tgagtcactt
     1381 ctcatttgtt gatgatgtct tctttattaa taaaatacct gaaggcttaa catttgatca
     1441 tgcatttgag tgtgtgggtg gtcgcggtag tcaatcagcc ataaatcaaa tgatcgatta
     1501 catttcacca gaaggaagca ttgcactgtt aggtgtaagt gagttcccag tagaagttaa
     1561 tacacgtcta gtattggaaa aaggactaac gttgattggt agtagtcgaa gtggttcaaa
     1621 agatttccaa gatgttgtag acttatacat tcaataccca gatattgtag ataaattagc
     1681 gttgttaaaa ggtcaagaat ttgaaattgc aacaattaat gatcttacag aagcttttga
     1741 agcagacctg tctacatctt ggggtaaaac agtattaaaa tggattatgt aaatagaaaa
     1801 ggatgaatta acgttggtta aaagtaagat atatatagat aaaatctatt gggaacgtgt
     1861 tcagttattc gttgaaggac atagtgaaaa cctagattta gaagatagta attttgtatt
     1921 aagaaattta actgagacac gtacaatgaa ggcgaatgat gtcaaaatag atgggaatca
     1981 attcgtttgt cgtttcaatg tagctatctt agataatggt tattacttgc ctgaagataa
     2041 gtacttatta gtgaatgagc aagaacttga ttatattgca cagttaaacc cagatgtgat
     2101 taatgatgca tatcaaaatc taaagccaga acaagaagaa gaatacaacg aattagaaac
     2161 acaaaatggt aaaatcaatt tcttattgca gacttaccta aaagaattta gaaaaggcgg
     2221 catttcgaag aaaacggttt atactgttac acctgaaatt tctagcgatg ttaatgaatt
     2281 tgtccttgat gttgttgtaa cgactccgga agttaaaagt atttatatcg ttcgtaaata
     2341 taaagaatta cgtaagtatt tccgcaaaca atcatttaat acaagacaat ttatttttaa
     2401 agcgatattt aatacgacga aatttttcca cttgaaaaaa gggaatacgg tgttgttcac
     2461 atcagactct agaccaacga tgtctggaaa ctttgaatac atctataacg aaatgttacg
     2521 tcaaaattta gataaaaagt atgatattca cactgttttt aaagcgaata ttacagatag
     2581 acgtggcatc atcgacaagt ttagattgcc atatttactt gggaaggcag actacatctt
     2641 tgttgatgac tttcacccat tgatttatac agtgcgtttt agacgttctc aagaagttat
     2701 tcaagtatgg catgccgttg gtgcctttaa aacagttggc tttagtcgta ctggtaaaaa
     2761 gggtggacca tttattgatt cattaaatca tcgtagctat acaaaagctt atgtatcatc
     2821 tgaaaccgat attccattct acgctgaagc atttggtatt aaagagaaaa atgtagtgcc
     2881 tacaggtgtt ccacgtactg atgtactatt tgatgaagct tatgcgacac agattaaaca
     2941 agagatggaa gatgaattac caattattaa aggtaagaaa gtcattcttt tcgcaccaac
     3001 atttagaggt agtggtcatg gtacagcaca ttacccattt ttcaaaattg atttcgaacg
     3061 tttagcaaga tattgcgaaa aaaataacgc ggttgtatta tttaaaatgc atccatttgt
     3121 gaaaaataga cttaatattg cagacaaaca taaacaatat tttgttgacg tttctgactt
     3181 tagagaagtt aatgatatac tgttcataac agatttatta attagtgact attcatcttt
     3241 aatatatgaa tatgcagtat ttaaaaagcc aatgattttc tatgcatttg atttagaaga
     3301 ttatattacg acgcgtgatt tttatgaacc atatgaatca tttgttccag gtaaaattgt
     3361 gcaatcattt gacgcattaa tggacgcctt ggacaatgaa gattatgaag gagaaaaagt
     3421 cattccattc ttagataaac attttaaata tcaagatggc cgatcaagtg agcgtttagt
     3481 cagaaattta tttggtagct aagtttatat agtagtcaaa gtgggagagg tataatgatg
     3541 aaattttcag taatagttcc aacatacaat tcagaaaaat atatgacaga attacttaat
     3601 agccttgcga aacaagattt tccgaaaact gaatttgaag tggttgtagt tgatgactgt
     3661 tcaacagatc aaacgttaca aatagttgaa aagtatcgta ataaattgaa cttgaaagta
     3721 agtcaactcg aaacaaattc tggtggtcca ggtaaaccta gaaatgtggc gttaaaacaa
     3781 gcagaaggtg aatttgtatt atttgtggac tccgatgact atataaacaa agagacttta
     3841 aaggatgcag cagcatttat tgatgaacat cactcagatg tcttattgat taaaatgaaa
     3901 ggtgttaatg gtcgtggtgt accacaatct atgtttaaag aaacagcacc tgaagttact
     3961 ttgttaaatt caagaattat ctatacttta agcccgacta aaatctatag aacagcatta
     4021 ctaaaagata atgacattta ttttccagaa gaattaaaga gtgcagaaga tcaattattt
     4081 acaatgaaag catatttgaa tgcaaatcga atcagtgtgt taagtgataa agcgtattat
     4141 tatgctacaa agcgtgaagg ggaacatatg agtagtgcgt atgtttcacc tgaagacttt
     4201 tacgaagtca tgagattgat tgctgtagaa atattaaatg cagatttaga agaagcccat
     4261 aaagatcaaa tcttagcaga atttttaaat cgtcatttta gtttttctcg tacgaatggc
     4321 ttctcactta aagttaaact agaagatcaa ccgcaatgga ttaatgctct aggagacttt
     4381 atacaagcag ttccagaacg tgtagatgca ttggtgatga gtaaattacg accattgttg
     4441 cactacgcga gagcgaaaga tatagacaac tatagaactg tagaagaaag ttaccgtcaa
     4501 ggtcaatact accgttttga tattgtagat ggtaaattaa acattcaatt caatgaaggc
     4561 gaaccatact ttgaaggcat tgatatcgct aagccaaaag tgaaaatgac agcatttaaa
     4621 tttgataatc ataaaattgt tacagagcta acgttaaatg aatttatgat tggcgaagga
     4681 cattatgatg tcagacttaa attacattca cgaaacaaga agcacacaat gtatgtacct
     4741 ttaagtgtca atgcgaataa acaatatcgt tttaacatta tgttagaaga tattaaagcg
     4801 tatttaccta aagaaaaaat ttgggatgtt ttcttagaag tccaaatagg tacggaagta
     4861 tttgaagtgc gtgttggtaa tcaacgtaat aaatatgcat atactgcaga aacaagtgca
     4921 ttaattcatt tgaataatga tttttataga ttaacaccgt atttcacaaa agactttaat
     4981 aacatttcgt tatactttac agctattaca ttaacggatt caatctcatt gaagttaaaa
     5041 ggtaaaaaca aaatcatttt aactggtctg gatcgtggtt atgtatttga agaaggtatg
     5101 gctagtgtcg tgctaaaaga cgacatgatt atgggaatgt taagccaaac gtcagaaaac
     5161 gaagtggaaa tcttacttag taaagatatt aagaagcgag acttcaaaaa tattgttaag
     5221 ttaaacactg cacatatgac ttactcgcta aaataaataa taaatgccct caaatctatg
     5281 tgaactaaca tgatttgagg gttttatttt actatttatg aaatgattat gacatttccc
     5341 tgattttcat tttcatatac attaaattgt atacactgaa aatgaggagg tcgtctataa
     5401 tgataaataa aaatgacata gtagcagatg tagtaacaga ttatccgaaa gcagcagata
     5461 tttttagaag cgtaggtata gacttttgtt gcggtggaca agtaagtata gaagcagcat
     5521 ccttagaaaa gaaaaatgta gatttgaacg aattattaca gcgtctcaat gacgttgaac
     5581 aaacgaatac accaggttcg cttaacccta aatttttaaa tgtttcgtca cttattcaat
     5641 atattcaagc agcatatcat gaacctctta gagaagaatt taaaaattta acaccttatg
     5701 tgacgaaatt atcgaaagta catggaccta accatccata tttagtcgag ttaaaagaaa
     5761 catatgatac atttaaaaat ggcatgttag agcatatgca aaaagaagat gatgttgatt
     5821 ttccaaaact aattaaatat gaacaaggtg aagtagtaga cgatattaat acagtgattg
     5881 atgatttagt atctgatcac attgcaacgg gacaattgtt agtgaaaatg agcgatttaa
     5941 catctagcta tgaaccaccg atagaggcat gtggtacgtg gcgactcgtt tatcagagat
     6001 taaaagcact tgaagtgtta acacatgagc atgttcattt agagaatcat gttttattta
     6061 aaaaagtatc ataaataacg cgattagaaa ctgttggcaa agtaatgtca gcagtttttc
     6121 gctatactta acagaaattt agtgatatga acagcattat ttgaaaagaa aaatggtcaa
     6181 cttagcataa aaattgatat gaaaatttaa tggtatagat aattaaatag tagcgtgttt
     6241 t
//
LOCUS       DAHHYJ010000144         6206 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000144 DAHHYJ010000000
VERSION     DAHHYJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.144"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(16..1182)
                     /gene="norA"
                     /locus_tag="NBK22_002245"
     CDS             complement(16..1182)
                     /gene="norA"
                     /locus_tag="NBK22_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCD2415420.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLRLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADISPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSIVLIHDPKKSTTSGFQKLEPQLLTKINWKVFITPVILTLVLSFGL
                     SAFETLYSLYTADKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     LYSVVVLILLVFANGYWSIMLISFVVFIGFDMIRPAITNYFSNIAGERQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKEQNM"
     gene            1391..1813
                     /locus_tag="NBK22_002246"
     CDS             1391..1813
                     /locus_tag="NBK22_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD2415421.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITTFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLDNGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            1999..2616
                     /locus_tag="NBK22_002247"
     CDS             1999..2616
                     /locus_tag="NBK22_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCD2415422.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLISHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            2613..2897
                     /locus_tag="NBK22_002248"
     CDS             2613..2897
                     /locus_tag="NBK22_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415423.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(3052..4425)
                     /locus_tag="NBK22_002249"
     CDS             complement(3052..4425)
                     /locus_tag="NBK22_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCD2415424.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVTAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDIAFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEANFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(4511..6064)
                     /locus_tag="NBK22_002250"
     CDS             complement(4511..6064)
                     /locus_tag="NBK22_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCD2415425.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAVLAGSDLLGTNHALSLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSVALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSPREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLYLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
BASE COUNT         2103 a         1121 c          890 g         2092 t
ORIGIN      
        1 accaaaatac ttatgctaca tattttgttc tttcaatttt gctctatgtt gcttttcaat
       61 taaaacaata acaacacctg ctaatgaaac acctatagcc atataaattg gtgcttcaat
      121 gtgtacatca aataacgcac ctgcgattaa aggacctatg aaattaccca tactagtgaa
      181 tgtcgagttc aatccgcctg caaagccttg cctttctcca gcaatattag aaaaataatt
      241 tgtaatggct ggtcttatca tatcgaaacc tatgaagaca acaaaactga ttaacattat
      301 tgaccaatag ccattagcaa aaactaataa tattaagaca acaactgaat ataataatga
      361 ccaagctata aatgttaact ctgagaaata cttcataaat ttatcgaaga aatagatttg
      421 gaaaagtgcc ccaaatatac cgccacccgt aatagcaatc gaaatatctt taggtgaata
      481 atttaccttg tcagctgtgt atagtgaata caatgtttca aatgcagata aaccaaacga
      541 taatacaagt gttaaaataa ctggtgtaat aaacactttc cagttaattt tcgttagcaa
      601 ttgtggctct aacttttgga aaccacttgt cgtagacttt ttcggatcgt gaatcaatac
      661 aattgacatt ataaatgcta gaatacctaa tgctcctgca aagtaaaatg gcatacgatg
      721 tgaaacttct gccataaatc caccaatccc tggtcctaaa atgaatccag aattgataat
      781 cgctgacatg tagccaaagt tttttgcttt ttgatggctt ggtgaaatgt cagctattaa
      841 acctgtcaca ccaggcatta ccataccagc actcatacca ccaatcactc tcgataacat
      901 caataccgaa aaattgtggc caactgcaaa cataaattct gacactgaaa acaaaattaa
      961 tcctatacat ataattaatt tcttccctaa tttgtcagct agcgtaccac caaacggcga
     1021 tataatcatt tgagataacg caaaagcagc aactagtaat cctaaatcac taccagttaa
     1081 tctcaaatct tttaaataaa caggcaagac tggtattact aaaccgatac ctaaaaaaat
     1141 caagaaaata ttaaaatata agacaaaaat ctgtttattc atatgctcac ctcttttttc
     1201 tccatgtcat gcttaaagct gttattcttc ttttccacga cagattgcaa ttcatgctaa
     1261 atattcgtaa aaagtttcta tattgtatta caacattgct acatttgaca atattttttc
     1321 tttgtaatta tcactatttt ccatttaatt gtataattaa tgacatatta aaataaaggg
     1381 gattgatcat atgaaattgc aaaaaagtaa tcatactata ctactagtgt tggaaaaagg
     1441 agaagatatc gttgaatgta taacaacctt tgcagatgac caagacttaa catttacatc
     1501 tgtcagcggc attggtgcat gtgatgacgt tgtattaaaa tttttcaatt taacaacgaa
     1561 acaatacgaa gaaaaacata ttactgagcc actggaattg acgagcttat taggtaatat
     1621 ttctcgtcta gacaatggtc attttgcaca tttgcatgct actttcggta ctcaatcata
     1681 tgaaactttt agcggacatc tcgctaaagc aattgtttct gcaactgctg aaatcattct
     1741 tactgtaact gatttggaca ttcaacgttc attcaaagat gcagtcggtt taaatttact
     1801 tgatcctcaa tagcacttta ctttttagtt gaatcaaatt ttattacaag ttatcataag
     1861 tgcttaatca taaatgcatt ctcgtttatt actatacttc aacgagttaa attaaatttt
     1921 taattaggaa ttgaatgcgt ttataactat cacatttgaa tgaataactt tttcattaga
     1981 aagtagtgtt tattattgat gcaatctcga tacatcgcta gaatttactt tttaatttta
     2041 tttatcgtct cactttttga gactaaaatt tttcaattta tgactttaaa tttgttttta
     2101 gcatatatac cttttgagct tagtctatta ttacgattat tcaaaccgaa taaaaagtat
     2161 gaatggccac tatttatcat atatagtttt atatttgtgt tacttttacc aaatacattt
     2221 tatatggtaa ctgatttaat ccatttgaac caatttcaat tcaactttta cgcaggacta
     2281 aatttaagtg agtggaaata ctttacctat ttactgcttg gtgtcttctt agcggtttat
     2341 gtgatgattt taatctattt tgaaatacta acattgattt ctcaccgttg gcttaataga
     2401 ttaattgttg tcgtgctaat gtttttaaat ggattcggaa tttatattgg tcgtttctta
     2461 cgcttacatt ccgtttattt ctttaatgag cccttacatg ttttaaatca cattctagaa
     2521 agtctaacgc ttaaaactgc tatgtttgta tgttttatgg ttgtcatgca agcagcaatt
     2581 ctattatttg gaaaaggagt gcgtttgaac aagtgacaat aaatataatc gttttaatag
     2641 tatctatcat tgtctttcaa ttgattattg gacacatatg gcatgatatt ggtttaagtt
     2701 atttaagaag tatcttactc atgatgctgc catttggact aggtgtattt atacaacaag
     2761 tgtcgtatta cgaacgacaa tatcctaaat ggcaagtgcc tcaaaacata aaagttagat
     2821 tgaaatatat ttatctcgct acgtttttag agtatgtcgt actatacctc actttattta
     2881 cagacatact aagataacat caaattaata agaaaactaa atacaactta tatatttgag
     2941 gaaagccaat ttctatcaca tcaataatag aaattggctt atttatattg cttaagaact
     3001 ttatattctg ctccagattt tactcatgcc agatctatca attgagccga attagtctag
     3061 cgctttaaat gtagctaaaa cttgtgtacg tttttcttga tgatctacca tttgtcttgg
     3121 ataatgacta cctaattcaa tcccctgttc aaaaaggttg gactcattgc gttgtgtatc
     3181 atgcaaatat tttgcatcaa tttgattaaa aatcggaaga tatgttttga tatacaaagc
     3241 tttagcatca aagcgttcac tctgtcttat tggattaaac attctaaaat acggcactgc
     3301 atccgtacct gtagaagcag accattgcca tccatgaata tttgatgctg catcatagtc
     3361 aataaggtgc tttctaaaga atttttctcc ccatgtccaa tctataaata aatctttggt
     3421 taaaaattgc gacacaacca ttctcattcg attatgcata aaaccagttt gtgtcaattc
     3481 cattattgct gcatcaatga ttggaaatcc tgtttgccct tcgcaccatg cattaaaatt
     3541 cgcttcattt tgcgaccatt ttatctgtcg atatttaggt ttgaaagctt ggtatgaggt
     3601 ttcaggatac tgtgtcatta acacataata aaattctcta aaaatgagtt cacgtataaa
     3661 tgcttcatag tttgcctcat cttcatcata acgcgccaat aaatcattaa aaatttcaat
     3721 aatatctaat aatccatatg ctaaagcaac acttagctga cttgttaata cttcaggtaa
     3781 atagtctctt ccgctttcgt aattctgtat atcttgatct aaaaaatgtt gccatttatt
     3841 ctgttcaatt atttgtgagg atccaaatgc tatgtcatca aacactaaat catcaggtga
     3901 tgcaatgacg aagtcttcga atgattttaa atcatagtga tatacgtctc tatgcctcaa
     3961 gtaaggtcgc cattttttat agaaactagt aaaaactttg tatggctccc cttgttgatt
     4021 cattgtttta ctcgctttaa agtaatgatt cccacgtatc aaagtaacgg caatgcgtgc
     4081 ttcattgaat aaagaacgtt gatgtaaaat atcatattct tcaagatgat aactcataat
     4141 atcacccgca gtcactactt cagagatatt gttttcttta caaaatgaaa tgatatcgtc
     4201 ataagacata acataaggtg taagcttttc tgcgtgcaac gattggtaaa agcgctgcaa
     4261 agtaccgcga taaaactgtg ctttcaaatc agaagctgaa gacatgtcct cttccggaat
     4321 gataaaataa catgcatcaa tgtcttcttt ttgttgataa atatattcaa ataatggatt
     4381 gtgttccatt cgaaacattc gatttaataa cacagcaatt gccataaaca aaacaccctt
     4441 caattatctt tttattttat taagcttttt gtcactttaa aataacctag attcttattt
     4501 atatttttaa ttaaagcaaa cctaaccatt tccaataagt catgctaaaa actatcacta
     4561 gaatataccc tacaattgtc aatggtatac ctgccttcaa gaaatcttta accgtaaaag
     4621 taccagtgcc ataagccaac atattttgag gtgcactaac aggtaataag aaaccaaaac
     4681 taataacaaa ttgttgaatt aaaacaaatc ctatagactg gtctcctaag tataacgtag
     4741 aggttagcga aataaaaaca ggtattaacg ctgatgataa acttgttgca ctcgcaaagc
     4801 ccaaatgaat caatatatta aaaagcgtga taagtgcaat tgtcgcgata ataggtaaat
     4861 gttttaaacc taaaacacca aaagtttgat cacttaacca ttgagctgca cctgttttca
     4921 aaagaacgtt acctagtgaa atacctacac caaacacgat aattgttccc catggtattt
     4981 tattttcaac atgtttccat gtcatgacac caattttcgg cattaacata acacctaaag
     5041 caataatagt aatggatgca gagtcaatcg gatgtaatac tttttcagtt gaccaaaaca
     5101 gtaataataa catcgatata acaattaaac gccattcacg tgggctaacg gggccaagtt
     5161 tatgcaattc ttcttttatt aaatctttac caccttctat tgtattaatt tctggaggca
     5221 tcactttaat catgatgaaa tataaagcta cggacataac tatggaccaa ggcgctgcat
     5281 ataagaacca ctcgccccat gaaacatcaa atcctaattg atggtttata aaattaatcg
     5341 ctacgatatt ttgtgctgcc gccgttttga taccaatatt ccaaattgac acagcttgta
     5401 ctgaagtaat tattaataaa gacgctaact tgctatcttt ggaaacttta aatgccgcaa
     5461 tcatacccag caagattggt acaactgccc ctgctctagc tgttgcagaa ggaacgaaaa
     5521 atgcaagtac aattgaaacg ataattgctc caataactat atttctagtt ttattaccaa
     5581 caattgataa cactaaaaga gctagtcttt tatgcaaatt cgtttcttgc atagcagcag
     5641 ccaaaaataa tgcggctgca acgagagcta cagctgaagt tgcaaatcca ctaaacgcta
     5701 atgataatgc atgattagtt cctagaaggt cacttccagc taaaactgca ctgccacttt
     5761 tcggattacc tagcttctcc cctaaatttt ggaccgggct aaatcctaaa agtaatatca
     5821 ttaagccaat aattaaagtt gctgacaccg gatatgatac agcttccgtt acccacataa
     5881 taactgcaaa agctaaaata gctagtactg ccttagccat tattggcaga cttgaaggtg
     5941 ccgggagtaa tagtacaact ataagtatta taaaactcat gacaatccaa aatggcttgt
     6001 attgcttttt tgtcttttct ttcttttgtt gttcatgttc cactacatgt ttttcttctg
     6061 acataacagt cacccctttg tttaatctta atttaattat aacaattttt taaaacatac
     6121 atttttaatt tatgcaaaat acacaatttc atcgaaatat tttgtaaata atattgctag
     6181 gtgatgagtt atctcggtta attaca
//
LOCUS       DAHHYJ010000145         6187 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000145 DAHHYJ010000000
VERSION     DAHHYJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.145"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            45..614
                     /locus_tag="NBK22_002251"
     CDS             45..614
                     /locus_tag="NBK22_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCD2415426.1"
                     /translation="MNTKFLGKTLVASALVLTTLGTGLHSSYLGLNTNKVVKTAKAEE
                     KMTNGQLWKKVKDSLIDSKFISGNDGESVKVTYLTKDGQSTHVESTANHDDVTNTPSN
                     FSKLSQIDITKENIPQEDFNTRLDANEAWHNITKKLLEKGLVSNGNKVIISTKDPKDP
                     QIAGTVGSEMNDHKGFNLNKRDINKITIE"
     gene            complement(1088..1376)
                     /locus_tag="NBK22_002252"
                     /pseudo
     CDS             complement(1088..1376)
                     /locus_tag="NBK22_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500445.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BsaG protein"
     gene            1562..2035
                     /locus_tag="NBK22_002253"
     CDS             1562..2035
                     /locus_tag="NBK22_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415427.1"
                     /translation="MHLKLNDEDLNYLVNLSKEILEQYNIKLDETCKGDINITSCNSE
                     YFFTLSYFLKPGKATINFRECRYNYSLLRLNLNDGFHKNSNNEKIRGNRINIFSENEF
                     IQKADGVTHMKAYPLPYKIFEDSSDFVKQLFTLLEYTKTNHNDNIKIETNLFMSW"
     gene            2040..2831
                     /locus_tag="NBK22_002254"
     CDS             2040..2831
                     /locus_tag="NBK22_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HCD2415428.1"
                     /translation="MHDNTIKSISKDYFDYLQTESKFIPLENETIEFYSPIVDYFGDS
                     ISVNISFSGDRYKLTDHGETLWNMEQLGIDLTSNKKQKKYQLLNNIVQNYSLVIENKH
                     INFYTNRINLSQAIHDYVLAISEISYLGILKKENIKSLFKDEVMQYFLSNRKIYPNIF
                     PEFKVEGKSKLIHSFEAVFPGEATEYVKTIKRIDKNNAKNVLFDWNDVEVHRNKNFDS
                     NARLNIITEQQDDISEAVSTMLSQYNVEVFSFENKKQLEEKFSNV"
     gene            complement(3188..4171)
                     /gene="lukD"
                     /locus_tag="NBK22_002255"
     CDS             complement(3188..4171)
                     /gene="lukD"
                     /locus_tag="NBK22_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit D"
                     /protein_id="HCD2415429.1"
                     /translation="MKMKKLVKSSVASSIALLLLSNTVDAAQHITPVSEKKVDDKITL
                     YKTTATSDNDKLNISQILTFNFIKDKSYDKDTLVLKAAGNINSGYKKPNPKDYNYSQF
                     YWGGKYNVSVSSESNDAVNVVDYAPKNQNEEFQVQQTLGYSYGGDINISNGLSGGLNG
                     SKSFSETINYKQESYRTTIDRKTNHKSIGWGVEAHKIMNNGWGPYGRDSYDPTYGNEL
                     FLGGRQSSSNAGQNFLPTHQMPLLARGNFNPEFISVLSHKQNDTKKSKIKVTYQREMD
                     RYTNQWNRLHWIGNNYKNQNTVTFTSTYEVDWQNHTVKLIGTDSKETNPGV"
     gene            complement(4173..5108)
                     /gene="lukE"
                     /locus_tag="NBK22_002256"
     CDS             complement(4173..5108)
                     /gene="lukE"
                     /locus_tag="NBK22_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114304764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukED subunit E"
                     /protein_id="HCD2415430.1"
                     /translation="MFKKKMLAATLSVGLIAPLASPIQESRANTNIENIGDGAEVIKR
                     TEDVSSKKWGVTQNVQFDFVKDKKYNKDALIVKMQGFINSRTSFSDVKGSGYELTKRM
                     IWPFQYNIGLTTKDPNVSLINYLPKNKIETTDVGQTLGYNIGGNFQSAPSIGGNGSFN
                     YSKTISYTQKSYVSEVDKQNSKSVKWGVKANEFVTPDGKKSAHDRYLFVQSPNGPTGS
                     AREYFAPDNQLPPLVQSGFNPSFITTLSHEKGSSDTSEFEISYGRNLDITYATLFPRT
                     GIYAERKHNAFVNRNFVVRYEINWKTHEIKVKGHN"
     gene            complement(6047..6135)
                     /locus_tag="NBK22_002257"
     tRNA            complement(6047..6135)
                     /locus_tag="NBK22_002257"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6098..6100),aa:Ser,seq:gga)
BASE COUNT         2232 a          992 c          816 g         2147 t
ORIGIN      
        1 ttttcattgt aaacgtttac aatatgaaaa aaggagcaat taaaatgaat acaaaatttt
       61 taggtaaaac attagtagca agtgctttag tattaacaac attaggaaca ggtttacatt
      121 cttcatactt aggattaaat acaaataaag ttgttaaaac agcaaaagca gaagaaaaaa
      181 tgacaaatgg tcaattgtgg aaaaaagtta aagattcatt aattgattcc aaatttatta
      241 gcggaaacga tggtgaatct gttaaagtca catatttgac aaaagatggt caatcaactc
      301 atgttgaatc aactgccaat cacgatgatg taactaacac tccaagtaat ttttcaaaat
      361 taagccagat tgatattact aaagaaaata ttcctcaaga agactttaat acaagacttg
      421 atgcaaatga agcatggcat aatatcacta aaaaattatt agaaaaagga ttagtttcaa
      481 atggtaacaa agttataata agtacaaaag atcctaaaga cccccaaatt gcaggaactg
      541 ttggatctga aatgaacgat cataaaggtt ttaacttaaa taaaagagac atcaacaaaa
      601 tcacaattga ataaacctta aattcaaatt tccttaattt ataaaacctt aataactaac
      661 cctttcttac aaaccaccta gcccataata tattaaaacg tcatttttat ttcaattagc
      721 ggaaaataaa aaaaccattc cgcattaact ttggcaagtt ggaatggtta aaaaaatatt
      781 ttagaagcca gcgtttggct tgctttgacg acatgttcgc gcatttcgtc ttatttttac
      841 atttatataa taacacgcaa ttcaataaaa tgtctaatca ataaacctca attacattca
      901 aattcaaaaa ctttgcacct aatattcatc gcttaaataa acattacgtt taattaacca
      961 gacattgtca atcgcatcta atacccctgc tgtccgaata caaaattatt ttgtattcat
     1021 atttatctgt tctctttcct cccttttaaa cgcaactaaa tttcttttac cattagcgcc
     1081 ccaatcttta atctttaatt gttctaccgc tccatttttt aaatccttta aagagtaaaa
     1141 ctgctaatag caacgtgata ataatataga tcgccaatgt taatgtaact ggtatactcc
     1201 cttctataaa catataaacg taacgtgtaa catatgtgat tggtaaatag aaccacgaat
     1261 gatctccaag cacttctaag ccaaaataga cgttaaaaat aaacattaaa attccggcaa
     1321 caatagccat tacatcttta atgaaaaata tatctaacat tatcacaaat tattaataat
     1381 gttaaaaatg acggtattaa ataaattaat aaatattcca ttgtcatgta ttgctatacg
     1441 attatgacgc taaaaaggaa taattattaa gtttatagaa aacatctgta taacttacta
     1501 caaatactga aaataatata aggatttaag atcgtaaaat ctaaactaag gagatgttta
     1561 aatgcatttg aaactgaacg atgaagattt aaattattta gtaaatctaa gtaaagaaat
     1621 acttgaacaa tacaacatta aattagacga aacttgtaag ggagatatta acataacatc
     1681 atgtaattct gaatactttt tcactttaag ctatttttta aagcctggaa aagcaactat
     1741 aaatttcaga gaatgccgtt acaactatag cttgttacgt ttaaatctta atgacggttt
     1801 tcataaaaac tctaataatg agaaaataag gggaaataga attaatatat tttctgaaaa
     1861 tgaatttata caaaaagctg acggcgtaac ccatatgaaa gcatatcctt taccttataa
     1921 aatatttgaa gatagctctg attttgtaaa acaattattt actttgcttg aatatacaaa
     1981 aactaatcat aatgataata ttaaaattga aactaatcta ttcatgagtt ggtgatacta
     2041 tgcatgacaa cacaattaaa tctattagca aagactattt tgattactta caaacagaat
     2101 caaagtttat cccattagaa aatgaaacta tagaatttta ttcacctatc gtcgattatt
     2161 ttggcgattc tatttctgta aatatttctt tttcagggga tcgatataaa ctaactgatc
     2221 atggtgaaac tttgtggaac atggaacaat taggtataga tttaactagt aataaaaaac
     2281 aaaaaaagta tcaacttctc aataatattg tacaaaacta cagtctagtc atcgaaaata
     2341 agcacataaa cttttataca aatagaataa acttatctca agctattcat gattatgttt
     2401 tggctatttc cgagatcagt tatttgggaa tattaaagaa agaaaatatt aagtccttat
     2461 ttaaagacga agtgatgcaa tattttctat caaacagaaa gatttatcct aatatatttc
     2521 ccgaatttaa ggtagagggg aaatctaagt taatccatag tttcgaagca gtatttccag
     2581 gagaagctac agagtatgtt aaaacaatta aacgaatcga taaaaacaat gcaaaaaatg
     2641 tccttttcga ttggaatgat gttgaagtac atagaaataa aaattttgat tctaatgcta
     2701 gacttaatat cattacagaa caacaagatg atattagcga agcagtatca actatgttat
     2761 ctcaatataa tgtagaggtg ttttcatttg aaaacaaaaa acaacttgaa gaaaagttta
     2821 gtaacgtata attatcgtgt tatatagtgt ttcttcgata atagtttcta tttcaattaa
     2881 atcaccgata cttaaaatga taaatttacc aataccaagt ttgtaatgaa ctttcaccgt
     2941 catacaatca tagatgctat atctacaaaa agaaccacgc cgattaagac gtggttccat
     3001 ttttttattt tggaagcttt taaataattc tactttcaca aatttagtac cctgatattt
     3061 agcaaagcaa tctacatttc cctaactaaa aatcatcatg catgtaaata aaaattatgt
     3121 tagttcttta attccgtttg ataattgaaa taattgcact ttgttgatta tttaatcata
     3181 tacaattcta tactccagga ttagtttctt tagaatccgt accgattaat ttaacagtat
     3241 ggttttgcca atcaacttca taagtagatg taaacgttac tgtattttga tttttgtagt
     3301 tattaccaat ccagtgtagt cgattccatt gatttgtata tctatccatt tctctttggt
     3361 aagttacttt gattttagat ttttttgtat cattttgttt atgagaaagt acgcttataa
     3421 attctgggtt aaagttacca cgcgccaata aaggcatttg atgtgttggc aagaaatttt
     3481 gaccagcatt tgaactactt tgtctaccac ctaaaaacag ttcattacca tatgttgggt
     3541 cataactatc tctaccatat ggtccccaac cattattcat aattttgtgc gcctcaacac
     3601 cccagccaat tgatttatga tttgtttttc tatcaatcgt agttctgtaa ctttcttgtt
     3661 tataatttat tgtttctgaa aatgattttg atccattcaa tccacctgat aagccattag
     3721 atatattaat atctccgcca taagaataac ctaatgtttg ttgaacttgg aattcttcat
     3781 tttgattttt aggtgcatag tcaacaacat ttacagcatc atttgattct gaactaaccg
     3841 aaacattata cttaccgccc caataaaact gtgagtaatt gtaatctttt ggattaggct
     3901 ttttataacc tgaattaatg tttccggctg ccttaagtac taacgtatct ttgtcataac
     3961 ttttatcctt aatgaaatta aacgttaaaa tttgagaaat attcaattta tcattatcag
     4021 atgttgctgt tgttttgtat aaagtgattt tgtcatctac ttttttctcg cttacaggtg
     4081 tgatatgttg agctgcatca actgtattcg atagcaaaag cagtgcaatt gatgaagcaa
     4141 ctgatgattt gactaatttt ttcattttca tattaattat gtcctttcac tttaatttcg
     4201 tgtgttttcc aattaatttc atatctaact acaaagtttc tatttacaaa tgcattatgc
     4261 tttctttctg cgtaaatacc agttctaggg aataaagtcg catatgtaat atctaagttt
     4321 ctaccatatg aaatttcaaa ttcactcgta tcacttgaac ctttttcatg tgatagtgta
     4381 gtgataaatg atggattaaa gccactttga actaaaggtg gcaattgatt atcaggagca
     4441 aaatattctc ttgctgaacc tgttggacca tttggacttt gtacgaataa atatctatca
     4501 tgcgcagatt ttttaccatc aggcgtaaca aattcgtttg ctttaacacc ccatttaact
     4561 gattttgagt tttgcttgtc tacttcactg acataactct tttgggtata actaattgtt
     4621 ttagaataat taaatgagcc attgccacct atagatggtg ctgactggaa attacctcca
     4681 atgttatatc ctaatgtttg accaacatca gtagtttcta ttttgttttt aggaaggtaa
     4741 ttgattaagc taacatttgg atctttagtc gtcagtccta tattatattg gaatggccaa
     4801 atcattcgtt tagtcaattc atatccacta cccttcacat ctgaaaatga agttctggaa
     4861 ttaataaaac cttgcatttt aacaattaaa gcatctttgt tatatttttt atcttttaca
     4921 aagtcgaatt gaacattttg agtaacgccc catttcttac tacttacatc ctccgtacgt
     4981 ttgattactt cagcaccatc accaatattc tctatattag tattagctct agattcttga
     5041 atcggagatg ctaaaggcgc aatcagtcct actgacaaag ttgcagctaa catttttttc
     5101 ttaaacataa gtttcacttt ctttctatat aaatttttaa tacaaattta ttctagatta
     5161 tttcacttat ttataaaatt aactgttcat taaaaaataa aatgctataa aatattaaaa
     5221 atgacacaat ttattcattt atgatgctga aaaccggtca ttttggcgca accttcatta
     5281 catttaaata gttcccttta tactcgaaca atatgataaa attgccgatg ctcaactttt
     5341 tcgctataaa aatcaaaacg aactttaatc aaatcttaaa aaataatgtt tgtattatca
     5401 aaaataatct ataattgaat ttaaaattaa ctgattatta actattgtcc gttaaacgga
     5461 gatgaaacaa cttatgttaa taaatcatat gaaaacaagt ctgttataac tgaaatcttc
     5521 tatatatcat ttcgtaatat cgctagcttc atctaaaaaa tttaatgcta tgatttagcc
     5581 gaattaaaaa catatatata tttactatga actaaaattt cactttaaaa ataaaaaacc
     5641 atgatttcga agtcttcata aactaaagct tctactctca tggcaattta tactatgata
     5701 ttcaattata atgcctagtc tcatagtaaa cggctactac attcgcatgt acttcgtttt
     5761 actgtccacg catggtcgtt gacttattta tattataata taaatatttt atttttcaat
     5821 acttaatttt aaaaatcccc tatgaaatca ttatgctaac gattaggtca ctcttcaata
     5881 tcacaaacgt taaacaccta atctcactta cttgaagaac ataaaaaaga gcaaggccaa
     5941 cgcgtcagaa agttattttc ttgacacgta tttgacactt gctagaataa aaagctgtta
     6001 tatcaacgtt ttgtttaacg agatttaacg agggatagga aaatagcgga gagtgaggga
     6061 ttcgaaccct cgagacgctt gtggcgccta cacactttcc aggcgtgctc cttcggccaa
     6121 ctcggacaac tctcctcaac aataaatatg taataaaaaa acagaagcga tatttcactt
     6181 ctgtttg
//
LOCUS       DAHHYJ010000146         6095 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000146 DAHHYJ010000000
VERSION     DAHHYJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.146"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            105..1592
                     /locus_tag="NBK22_002258"
     CDS             105..1592
                     /locus_tag="NBK22_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415431.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAIIYKEKQPVSVTVLF
                     YVLCFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIATIGTIIMFSNP
                     NYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSMIAALIIYLLLR
                     SDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFVNVIVCGYFAFS
                     IFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVYTIHVIILIILL
                     KEFRKYKSINVNFIKGASLVLAVIYLSAFIYVHYEHEKRVQLLKQQIKDHPRQEVYTV
                     ESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
     gene            1886..4516
                     /gene="polA"
                     /locus_tag="NBK22_002259"
     CDS             1886..4516
                     /gene="polA"
                     /locus_tag="NBK22_002259"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCD2415432.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTFEIETSFDNIDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYVELVSWLENPNSKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            4532..5404
                     /gene="mutM"
                     /locus_tag="NBK22_002260"
     CDS             4532..5404
                     /gene="mutM"
                     /locus_tag="NBK22_002260"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCD2415433.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            5420..6043
                     /locus_tag="NBK22_002261"
     CDS             5420..6043
                     /locus_tag="NBK22_002261"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCD2415434.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
BASE COUNT         2205 a          793 c         1102 g         1995 t
ORIGIN      
        1 gaggaaacat gcataggtga aaattagaaa tacattaaaa atattgaatt aatttttcga
       61 cctacaagtt gaggaaaatt taactagatt tgaggattag caaagtgaaa acaattcaat
      121 tacacaagac aactttgata ttaattgcga tattattgtt ttatactttt atgggtatct
      181 tactaccact catgcacgat gatttacaat ggtttagcaa ttataatact gatattttaa
      241 aagtaggatt tgcatcactt aatggtcgct atatcggaaa tatctttgaa attatagctg
      301 tacatgtaag ctggttacgt tggctttcat atggtctcat tagtatgggc atcatttgga
      361 tgattatgca cattacacgt tgtaaagcgt ggacaagcta ttatttgtta gcattttctt
      421 taatgttaat tttaccaagt gctatttatg cagatacata tggctggttt gcaggatttt
      481 ataattatgc gacatcaaca ctaatttcac tctttattat ttattattgt attaatgcaa
      541 ttatatataa agaaaagcag ccggtaagtg ttactgtact attctatgtt ttatgcttct
      601 tcggtcaatt atttatggag aatgtgacat tattccactg tatcatttta attttagctt
      661 ttctatatga atttactgca aatcgaacac ttaattataa attattgttt tcatttatga
      721 ttgctactat aggtacaatc attatgtttt ccaatccaaa ttatcgcaaa attttatttg
      781 aaggatcaga atatcaacaa gtttcaaata atcaaggtat cttttcaaaa tttgcagaaa
      841 tgatatcaac gtcacttcca tatggtgtca ttttcagtca gattataatt ttaagtatga
      901 ttgcagcgct tattatttat ctactgctta gaagtgatcg gtatgtacat ttaacaatat
      961 taaagcgtcg tatcattatg ataggattta tcactttacc gctttattat ttactatttt
     1021 acaatcagtt tttattaaat aaaaatacag atattggatt ggttggtttt gtgaacgtaa
     1081 ttgtatgtgg ttactttgcg ttttcgatat ttgtaggtat ttatttgtct attaatgatc
     1141 gtaaaacaca aatcacttta tattccttac ttattgcaat atgtgtttcg gcgtcgacac
     1201 tagtcattgt tactccaata gaaccaggta attttttgat tgtttatacg atacatgtaa
     1261 tcattttaat tattttatta aaagaatttc ggaaatataa gtcaatcaat gttaatttta
     1321 ttaaaggtgc atcactagtt ttagctgtga tatatcttag tgcatttatt tatgttcatt
     1381 atgaacatga aaaaagagtt cagttattaa aacaacaaat taaagatcat ccacgacaag
     1441 aggtttatac agtggaatca ttgccgtttg aacattatat gcatcatccg tcaccaatca
     1501 ataaaaatta ccaagagtgg tttaataatt atgaagaatt accgaaaatg accaaagtta
     1561 aatatattcc atttggttca gacgattcat aacttattct tacctataaa aatttaaaat
     1621 agcagcataa aaatattaaa aagtagacat aatcatgaaa tgataatgtg catctcaaaa
     1681 gttggacttt taacctgact ttatggagtg ctcatcaaga acaatcatga ttatgtcttt
     1741 tttatttttt agctaagtct atataggcac ttgttttatt tgaaattagg tcgtgtataa
     1801 atactctatg agatagtttc tttaattacg acatcacagt cgaatatgct aaaatatata
     1861 taaattagtt ctggaggtta aaattgtgaa taaattagta ttaatcgatg gtaatagctt
     1921 aagttttaga gctttttatg cattaccttt gttatcaaac aaagcaggca ttcataccaa
     1981 tgcagtatat ggttttgcaa tgttactaga gaagatttta aaagaagaaa agccaaatca
     2041 ttttttagta gcgtttgatg caggtaaaac gactttcaga catgaaaaat atagtgaata
     2101 taaaggtgga cgccagaaaa cgccgcctga actaagtgag caatttcctt atattcgcca
     2161 attattagat gcttatcata ttaaacgtta tgaattagat aactatgagg cagatgatat
     2221 tatcggaact ttaagtaaag aagcagataa agcgggattt cagacaatta ttattacggg
     2281 agaccgagat ttaacacaac ttgcaacgga taatgtaacc atttattaca ctaaaaaagg
     2341 tgttactgat gttgatcatt atacacctga ctttattgcg gaaaaataca acgggttaac
     2401 acctaatcaa attattgata tgaaaggatt aatgggagat acttctgata atataccagg
     2461 tgttgcaggt gttggagaga aaacagcaat aaaattgcta aaccaatttg acacagtaga
     2521 aggtgtctat gaacatttag atgaaatttc aggtaaaaag ttaaaagaaa aacttcaaaa
     2581 tagcaaagaa gatgcattaa tgagtaaaga attagcaacg attaatgtag atagtccgat
     2641 tgaagtaaaa cttgaagata cgttaatgac tcatcaagat gaacaacaag aaaaaatcga
     2701 attgtttaag aagttagaat tcaaacaatt gttggctgac attgatcaat cagcaagcgt
     2761 tgaagatgca atagaaaaga catttgaaat tgaaacgtct tttgataata ttgattttac
     2821 ttcattgaaa gaagcggcca tccatttcga attagacggt ggtaactatt tgcgaaataa
     2881 tattttaaaa ttctctttat ttacaggtga gaaacatatt gtaattaatg cggatgacat
     2941 aaataattat gtcgaactag tttcatggtt agaaaatccg aattcgaaaa aagtcgtata
     3001 tgatgctaaa aaaacatatg tagcatcaca tagattgggg attgatattc aaaatatttc
     3061 tttcgatatt atgttggcca gttatataat tgatccatct cgtacgatta gtgatgttca
     3121 atcagttgtt tcattgtacg gtcaaagttt tgtgaaagac gatgtgagta tatatggaaa
     3181 aggtaagaaa tttaaggtac ctgaagatga tgttttaaat ccatatgttg cttctattac
     3241 tgatgcaatt tactttgcga aaccaaatat ggataaacaa ttagaagaat acaatcaggt
     3301 agaactcttg gctgatttag agctaccgct agctaagatt ttaagtgaaa tggaagaaat
     3361 tggtatattt acagatgttc atgatttaga agaaatggaa aaagaaattc aagaaaaatt
     3421 agacgtcttg attcgaaata tccatgatgc agctggtgaa gattttaata taaattctcc
     3481 taagcaatta ggtgttgtat tgtttgagac attacaatta cctgttatta aaaagacgaa
     3541 aacaggatat tctactgctg tagatgtctt agagcaattg caaggtgaac atcctatcat
     3601 tgattatatt ttagaatatc gtcaattatc taagttacag tctacttatg ttgaaggatt
     3661 gcaaaaggta attagtgatg atcaacgtat ccatacacgt tttaatcaaa ctttagcgca
     3721 aactggacgt ttatcaagtg tagatcctaa tttacaaaat attccggtta gacttgaaga
     3781 agggcgtaaa ataagaaaag cctttaaacc aacttcaaaa gatagcgtta tattatcagc
     3841 agattattct caaattgaat tgcgtgtatt agcacacatt acacaagatg agagtatgaa
     3901 agaagcattt atcaacggcg atgatattca tacagcaact gctatgaaag tatttggtgt
     3961 agaagctgat caagtcgata gtttaatgcg tcgtcaagca aaagcagtta actttggaat
     4021 tgtttatggg ataagtgatt atggtttaag tcaaagttta ggtattactc gtaaaaaagc
     4081 aaaagcattc attgatgatt atttagctag tttcccaggt gtaaaacaat atatgtctga
     4141 tattgtaaaa gatgccaaag ctttaggtta cgtggaaaca ttgctacatc gtcgacgcta
     4201 tattcctgat attacgagtc gtaactttaa tttacgcggc tttgctgaac gtactgctat
     4261 gaatacgcca atacagggca gtgctgcaga tatcattaaa ctggcaatgg ttaaatttgc
     4321 tcaaaaaatg aaagagacaa catatcaagc taaactatta ttacaagtac acgatgaatt
     4381 aatttttgaa gtacctaagt cagaagtaga ttcatttagt gaatttgtag aagagataat
     4441 ggaaaatgca ttgcaattag atgttccatt aaaagtagac tcaagttatg gtgcaacttg
     4501 gtatgatgca aaataaagtg aggttaatgg tatgcccgaa ttaccagaag tagaacatgt
     4561 aaaaagagga attgaaccct acgttataaa tcaaaaaatt gaacacgtta tattttcgga
     4621 taaagtaatc gaaggtaaag ctcaaggcaa ggaaacaatt ataaaaggaa ttgaattaga
     4681 tacttttaaa actttatcgg aaggctatac cattacaaat gtagaacgaa gaagtaaata
     4741 tatcgttttt cagctagata ataaacgaga acaacgtaca ttaattagtc atttaggtat
     4801 ggcgggcggt tttttcattg tagatgaact tgaagatatt atgataccta attatcgtaa
     4861 gcattggcat gttatttttg agttgtcaaa tgacaaaaag ttaatttatt cagatatacg
     4921 tcgttttgga gaaattagaa atgttgcgtc tgtagcatct tatccttcat ttttagaaat
     4981 agcgcctgaa cctttttcaa atgaagcatt gacgtattat ttaaatcgaa ttcatcaaca
     5041 gtctaataag aataaaccaa tcaaacaagt gattttggat cataaagtaa tcgcaggttg
     5101 tggaaatatt tacgcatgtg aagcgttgtt tcgtgcaggt gttttaccag ataaaaaagt
     5161 aaaagactta acacaccaac aacaagaaat ggttttttat tatgttagag aagtactaga
     5221 agaaggtatt aaatatggcg gtacaagtat ttcagattat cgtcatgctg atggaaaaac
     5281 tggtgagatg caattgcatc ttaatgtata taagcagccg gtatgcaaag tgtgtggaag
     5341 tcaaattgaa acaaaaatca tcgcgacaag aaatagtcat tattgtccag tatgtcaaaa
     5401 ataaagaaag agtgaaatta tgccgaaagt tattggtcta acaggtggaa tcgcctcagg
     5461 aaaatcaaca gtatcagaac tcttatccgt attcggtttt aaagtagtag atgctgataa
     5521 agcagccagg gaagctgtta aaaaggggag taaaggttta gctcaagtac gagaagtctt
     5581 tggtgatgaa gcaattgatg aaaatggtga gatgaatcgt cgttatatgg gtgatctagt
     5641 gtttaatcat ccagaaaaac gcttagaatt aaatgctatc atacatccta tcgtgcgaga
     5701 tattatggaa gaagaaaagc aagaatattt aaaacaagga tataatgtaa tcatggatat
     5761 tccattatta tttgaaaatg aattggaaaa tacagtagac gaagtgtggg ttgtatacac
     5821 ttctgaaagt atacaaatgg atcgtttaat gcaacgtaat aatttgtcat tagaagatgc
     5881 gaaagcacgt gtctatagcc aaatttctat tgataaaaaa agccgaatgg ccgatcatgt
     5941 tatcgataat ttaggggata aacttgaatt aaaacaaaac cttgagagat tgttagaaga
     6001 agaaggttat attgaaaagc cgaattacgg agaagaagat taatattaca ctataaataa
     6061 gtcattactt tacgtacgcg ttgatgtatg taagt
//
LOCUS       DAHHYJ010000147         6055 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000147 DAHHYJ010000000
VERSION     DAHHYJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.147"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            132..299
                     /locus_tag="NBK22_002262"
     CDS             132..299
                     /locus_tag="NBK22_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5325 family protein"
                     /protein_id="HCD2415435.1"
                     /translation="MFWVFSILAVVFLVLFSFAVGASNVPMMILTFILLVATFGIGFT
                     TKKKYRENDWL"
     gene            complement(453..1280)
                     /locus_tag="NBK22_002263"
     CDS             complement(453..1280)
                     /locus_tag="NBK22_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCD2415436.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLSQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYITPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTISGPNSILVGNRGVHREILDVYLEPHHDALIQLHEQRFKRKSK"
     gene            1437..2051
                     /locus_tag="NBK22_002264"
     CDS             1437..2051
                     /locus_tag="NBK22_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCD2415437.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATNKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            2235..3587
                     /locus_tag="NBK22_002265"
     CDS             2235..3587
                     /locus_tag="NBK22_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCD2415438.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQS
                     "
     gene            complement(3669..4088)
                     /locus_tag="NBK22_002266"
     CDS             complement(3669..4088)
                     /locus_tag="NBK22_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD2415439.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGNVSLDMLQTTMGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWVIVMIMLISKKESKETTHKDDEYIYH"
     gene            complement(4371..5390)
                     /locus_tag="NBK22_002267"
     CDS             complement(4371..5390)
                     /locus_tag="NBK22_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD2415440.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDERTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            complement(5464..>6055)
                     /locus_tag="NBK22_002268"
     CDS             complement(5464..>6055)
                     /locus_tag="NBK22_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD2415441.1"
                     /translation="YNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERD
                     LTKLAPQVRGVVGDEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWF
                     DNMVIPKTAQNKEGAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEVKDDHRF
                     YPTKKEQERLEVYKDLGPEVLSEYNENFLNFKMSLK"
BASE COUNT         2006 a         1028 c          862 g         2159 t
ORIGIN      
        1 gccatataaa tagtttcctt ctttcttaag taaaatccca cttttaatta caactttttt
       61 attatatcat ataattgaga aaagataagc aaaaggtggg gataggatga aacaaaaaaa
      121 aatctaaaaa tatcttttgg gtattttcta tattagctgt tgttttctta gtattattta
      181 gttttgctgt tggtgcatca aatgtaccaa tgatgatttt aacatttata ttactcgttg
      241 caacctttgg aattggattt actacaaaga aaaaatatcg agaaaacgat tggctataag
      301 ttatatcgac aataacacgc gttgattaaa gaccacgcat gttcatgatg aacatttaaa
      361 tggttttata ttggcgcgtg tttttattat ttaagatata aaagcgtaat atgttaccta
      421 attgttagga ctacatatta cgcttttggt aattattttg attttctttt aaatcgttgt
      481 tcatgtaatt gtattaacgc atcatggtgg ggctctaaat atacatcgag gatctctcta
      541 tgaacaccac gatttccaac taagattgaa tttggaccac taattgttaa tggttctcct
      601 agtaaatttg aagcttgtcc atttacttca tataaaataa ccaatccgcc agcaaaatcc
      661 catggttgaa gtcttggcgt tatatatgct tctaaattac ctgtagcaac tgaaacgatt
      721 tcaagcgctg cactaccata tgcccttgca cttctagaat cattaacaat ttctttaaaa
      781 atttctccta aaattggttt agttaaccag ttcggattga tcccaataat gctttgactt
      841 agattagaat cattcaatgg tttcaagggt tggctacctc gatatgcacc ttcccctact
      901 ttagcatgat ataagacatc agccataaca tcatatacaa aacctgcata aggtttacca
      961 tcgatataaa taccaattga aattgcgaaa ttttcttgtt gatgaacaaa attcaatgta
     1021 ccgtctattg ggtcaacaac ccataccgta cctttggaag tatcgatgtc atgaccatgc
     1081 ccttcttcac ctaatacttg atgattggga tatgtttcta aaattgtatc aaaaataaaa
     1141 tcttctgttg ctttatctac atttgtaaca aggtcattcg gatttgactt tgtttcaatt
     1201 gttaaatttt gctccatcaa ttgtttaatt ctaattcctg cttcttgaat aagtccttgg
     1261 gcaaatccat aaagtgccat aacatcatct cctaacatct ttattataca tcaacatttt
     1321 ataaaaataa cttcttatga taaaaatgaa aatactaatt ttaatgagag cgtcttaagc
     1381 cgcaattgat aaaacatatg ctacaatatt tttaaacact atacaggagg taccctatga
     1441 caaaatatac atttaaacct aaagatttca aagcgttcaa cgtagaaggc ttagacgcac
     1501 gaatggaagc tttaaacgaa tacatacgac cacaactcca tgaattagga gaatatttta
     1561 gtgacttctt tacaagtcaa actggtgaaa cattttatcc tcacgtagca aagcatgcta
     1621 gaagaagtgt gaatcctcct aaagatacat gggttgcttt tgcaacaaac aaaagaggct
     1681 ataaaatgtt acctcatttc caaattggta tgtttgaaga tcaactgttt gttatgtttg
     1741 gaatcatgca tgaagcaaaa gataaagcaa cacgtgcaaa agtttttgaa agaaaattta
     1801 aagctattca acaattacct gatgattatc gtgtttgctt agatcatatg aaacctgata
     1861 aaccatttat taaagattta acggatgatg atttaaaaga agcgatacaa agagccatca
     1921 atgtaaaaaa aggtgaattc tttatagcgc gtgcaatcac accacaagat aaaagattaa
     1981 aaagtgacaa agcatttatt gcatttttag aagaaacctt cgatcagttc ttaccatttt
     2041 attctgcata aataactttg tttaaataat agagcacgta atcacatcca tgatttcgtg
     2101 ctcttttttc ttaatattaa atcgaacgtt caacataata attcatactt ttaaaaaaat
     2161 taaaataaat ttaggttgac ctaaacattt tattaggtta ttatattgtc cataagaagt
     2221 agaggtgagt caaaatgaat aataaacgac attcaacaaa tgaacaatta agtttagacg
     2281 aaataaacaa tacaattaaa ttcgatcatc gcagttcaaa taaacagaaa tttttatcat
     2341 ttcttggacc tgggttatta gtcgctgttg gttacatgga tcccggaaac tggataacat
     2401 caatgcaagg tggcgcccaa tatggctata ctttgctatt cgtaattctt atttcaagtt
     2461 tatcagcaat gttacttcaa agcatgacag tgagattagg aatagcaaca ggtatggact
     2521 tagctcaaat gacacgacat tatttatcaa gacctattgc tataatcttt tggatcattg
     2581 cagaactagc aattatcgct acagatattg ctgaggttat tggtagtgct attgctctta
     2641 atctcctatt taacatacct ttaatcgtcg gtgcactaat aactgtactt gatgtatttt
     2701 tactactttt tataatgaaa tatggtttta gaaaaattga agctattgtt ggtacattaa
     2761 ttttcacagt gttattcatc tttatatttg aagtctatat ttcatcacca cagttgaatg
     2821 ctgtgttaaa tggatttata ccacatagtg aaatcattac aaataacggc attctctata
     2881 ttgcattagg tattattggc gctacaatta tgcctcataa tttgtactta cattcatcaa
     2941 ttgtacaatc tagaacatac tcaagacata acaatgaaga aaaagcgcaa gcgattaaat
     3001 ttgctacgat agattcgaac attcagttat caatcgcatt tgtagtcaat tgcttattat
     3061 tagtgttagg agcatcacta tttttcaact caaatgctga cgatttaggt ggtttctatg
     3121 atttatatca cgccttaaaa actgaacctg tactaggtgc aacaatgggt gcaatcatga
     3181 gtacattatt tgcagttgca ttattagcgt caggtcaaaa ttcaacgatt actggtactt
     3241 tagcaggaca aattgtaatg gaaggatttt taagattaca cataccaaat tggttaagac
     3301 gtttaattac acgttctctt gctgtcattc ctgttatcgt atgcttaatc atttttaaag
     3361 gtaatgcagc taaaattgag caactacttg tattctctca agtattttta agtattgctc
     3421 tgccattctg tttaatacca ttacaattgg ctactagtaa taaagacttg atgggacctt
     3481 tctataataa aacatgggtt aatatcattt catggacact tattattatt ctaagtattt
     3541 taaatgtcta tttaatcgtt caaacattcc aagaactaca aagttaaaaa taaaaccatt
     3601 aaaatgttgg ttgtatctaa taattaacaa cttaacattt taatggtttt tcttttgcta
     3661 tgaacatatt aatgataaat atactcatcg tccttatgtg ttgtttcttt tgattctttt
     3721 ttagaaatca acataatcat cacgataacc cataagacaa ttgcaattaa attaagagcc
     3781 atcaaactta caattatcgc gataacaaaa agtataatcg ataattttct acgcccttta
     3841 atacaaattg tcgcaaatat accataaagt gttgaaatta ataaaataat tgctaatgca
     3901 cccattgttg tctgaagcat atctaaagaa acattgccac cgttctgaga taattgcttc
     3961 gctaattctt cacgttgtga tgcattacca ctttttaaag aaaaatagga tagccccata
     4021 actaataaat aaataatact aatactattt gcaatccaag ccatgattaa ttcaggtttt
     4081 ctattcatct taaaatcctt tctttgttta atattttaat aagtatacta cgagcctata
     4141 gatatacgca cattaattta cttgtatatt aacatatttt tcatctaatt tttataataa
     4201 gcatctgaat tcagattgat ggtaatttag gtgtatggta atgagaaatc gtcatttaaa
     4261 attatgacac ctaaacactt taattaaacg ttatcgataa ttaacctcat ataaaaaccc
     4321 agattaataa gcaaaagacc tctgcttact aatctgggta tcatcaatat ttattcttgc
     4381 tcttttttgt ccttaacttc acttgaattg cgttcttcag aaatatcatt gttgtaaatt
     4441 tgacgtcgct cttcaaaatt cattcgacgt tgactaactg cgctaggttg atttttccta
     4501 cgttctttca ttcgttggtt ataagcttta cgtttttctt ttttcttagc tttaatttca
     4561 gctttttctc gcttttgttg tgctttcaat tcttttggat caacttcagg ttctgcgtaa
     4621 taaccatcat cttcaatacc tagttcttca gtactttttt gtttgtattt agcctgtctg
     4681 gataatactg atggttcttc ctcagtgcgc tcatcatgat tatattcact ttcaactgca
     4741 cgtttaggaa attgatcata tttatctctt tcctgttctt gatcatatac agtatttgat
     4801 ttatccaatt ctttttcata tttaggcaca tcataataat catctttcgg ttgggggcgc
     4861 tcatacacag gttcacgata atcataaatc ggtccgttat actcttcata atattgtggc
     4921 gcaaccattt caactttatc ttttcttata aatagcatca tcgcaataat aaagaataat
     4981 atcggaatga ttaaagtaac gataagtaaa actaaaggaa tcgttacaat tgctgatatt
     5041 aagaataaga aacctgacaa gattcttata ttcatagata tgagagctaa aaatgaaatc
     5101 aacaaacaaa caataaagta aacgataata gcccatatac cattttgtaa ccatattaca
     5161 aattgtgttg tattatagcc atttccagct aataattgct ggataaatgc attattgttt
     5221 aatgtatttt caagattagc aatcgatgtg ttattactga atgaaacaag tgctataaac
     5281 atcgtaatga cagtaagcac taataacatc acccaacata accaacctag aactttttca
     5341 gttaatctac ttactggacg tttaatttga gtaaattgtt ctccagtcat tcgttacaac
     5401 tccttatagt acttatcccg ttattataac taaatataca gtaaataact actatttatg
     5461 attttatttt aatgacattt tgaaattcaa aaagttttca ttgtattcac ttaaaacttc
     5521 aggtcctaaa tctttataaa cttcaaggcg ttcttgctct ttcttagtcg gataaaaacg
     5581 atggtcgtct ttaacctctt taggcaacaa ttgtcgagca gccttgtttg gcgttgcata
     5641 gcctacgaat tctgtatttt gcttgttatt tttagcatct aataaaaaat tcataaattt
     5701 atatgcaccc tctttatttt gtgccgtttt tggaattacc atattgtcga accataaatt
     5761 cgatccttct ttaggaataa cataattata tttatcccct tcttgcacta gaggtgctgc
     5821 aacaccactc caaacaaccg ctatgttacc ttcattttgt tgaagcatca tggtaatttc
     5881 atcacctacg acacctctta cttgtggtgc tagtttggtt aaatctcgct ctgcttcttt
     5941 taaatggtgc gaattacggt cattaagatt atacccaagt ttattcaaac tcatgcctat
     6001 aatctctcta gcaccgtcaa ctagtaaaat ttggttttta aatttaggat tatac
//
LOCUS       DAHHYJ010000148         6001 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000148 DAHHYJ010000000
VERSION     DAHHYJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.148"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            94..369
                     /locus_tag="NBK22_002269"
     CDS             94..369
                     /locus_tag="NBK22_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCD2415442.1"
                     /translation="MAKQATMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            683..1909
                     /gene="ftsW"
                     /locus_tag="NBK22_002270"
     CDS             683..1909
                     /gene="ftsW"
                     /locus_tag="NBK22_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCD2415443.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFIIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            2462..5914
                     /locus_tag="NBK22_002271"
     CDS             2462..5914
                     /locus_tag="NBK22_002271"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCD2415444.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGESVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATV"
BASE COUNT         2102 a          843 c         1138 g         1918 t
ORIGIN      
        1 ctaacgacac attttaaatc tacttgcttt ttaatattta gtaagataaa cttttagtaa
       61 gacttgagaa tttatataga gggggagcgt gtcatggcga aacaagcaac aatgaaaaat
      121 gcagctttga aacaattgac taaagatgct gatgaaatct tgcatctgat taaagttcaa
      181 ctagataatt taacattacc ttcatgccca ttatatgaag aagtactaga tacacaaatg
      241 tttggacttc aaaaagaagt tgattttgct gttaaattag gtttagttga ccgcgaagat
      301 ggcaaacaaa ttatgttacg tcttgagaaa gaactttcaa aattacatga agcttttaca
      361 cttgtttaag gtttaatata taagcatatt aaaatggcgt gagatatcga caattgagct
      421 gcgccgattt tatatttata gtttaattac tgattaaagt ggctgttatg ttgtcttctt
      481 gatgacatgt cagtcacttt tattgggttt tagcaattca cgatattaag taaacaaata
      541 ttctataaat tacctcaaga gacctgttaa tatttgtgat tatgactgtg tcatgtatat
      601 aataagtaac atggattttt aaaattaaag tataaaacga aatcatttga agtataaatt
      661 gtagaattgg atgactgaat atatgaagaa ttttagaagt attttacggt atattggtaa
      721 aacctcaaag tttattgatt atccgttatt agttacatat attgtattga gtttaattgg
      781 attagtgatg gtatatagtg caagtatggt tccagcaact aaaggcacat tgactggtgg
      841 tatcgatgtt ccaggaacgt atttttacaa ccgacaatta gcatatgtca taatgagttt
      901 tataattgta ttttttattg catttttaat gaatgttaaa ttactgagta atattaaagt
      961 gcaaaaaggt atgattataa ctatcgtctc actattatta ctgacgttag taataggtaa
     1021 agatattaat ggttctaaaa gttggataaa cttaggattt atgaacttac aggcatctga
     1081 gttattaaaa attgcaatca tattatatat cccatttatg atcagtaaaa aaatgcctag
     1141 agtattaagt aaaccaaaat taattttaag tcctattgta ttagcattag gttgtacgtt
     1201 tttagttttc ctacaaaaag acgtagggca aacattacta atattaatta ttttagttgc
     1261 gatcattttt tattcaggaa ttggggtaaa caaagtccta agatttggta taccagcagt
     1321 actaggattc ttagtagtat ttgtcattgc attaatggct ggttggttac caagttattt
     1381 aactgccaga tttagtacgc taacagatcc attccaattc gaatcaggaa ctggatacca
     1441 tatttccaat tcattgcttg cgataggtaa cggtggcgta tttggaaaag gattaggaaa
     1501 tagtgcaatg aaattgggct atttaccaga accacataca gattttattt ttgcaattat
     1561 ttgcgaagaa ttaggtttaa tcggaggatt gctagttatt actttagagt tctttattgt
     1621 atatcgtgcc ttccagtttg caaataaaac atcatcatat ttttataaac ttgtgtgtgt
     1681 tgggattgcc acatactttg gaagtcaaac gtttgtaaac attggcggta tttcggcaac
     1741 aattccatta actggtgtgc cattgccatt tatcagcttt ggtggatcat caatgattag
     1801 tttaagtatt gctatgggat tacttctgat tgtaggtaaa caaatcaaag tagaccagca
     1861 acgaaagaaa caacaacaaa aagttgatat aagaagacaa tttaattaaa aaatactagc
     1921 caatatttag tacgagatga cttctgtcat taagcaatgc taaacattgg ctatttatat
     1981 actctttgtc aaattggact gatgagttca ataacggaag ttaggcaacc aaacaacgct
     2041 taacttcctt tttgttttga tattacgaga ttactcatga ttatcaaatt ttataaacaa
     2101 aacaacgtat aattataact aaattttatg actaaaaatt actaaatgtg gtggcgaaat
     2161 tagaatcatg atgataagat aaagttatcg ctattgataa taaatgttga tgttttgcaa
     2221 ttttattttt aattttttag agttgtagaa atcgaaaatg tttcacaaca aaaacgtgaa
     2281 ctacctgttg ttgcatagtc atttttgaaa ttattatttc tgaagcggag gttttaattg
     2341 tctcaagaat gttaaatttt gaaaatttga aacataacta tagcaaaaca gagcgcttta
     2401 agataaaatt ttattatcta attagttaga aaattctaat agtttgagga gtgtatcgct
     2461 attgaaacaa ataaaaaagt tacttgttgc taaccgtgga gaaattgcaa ttcgtatatt
     2521 cagagcggcg gcagaattag acatcagcac agttgcaatt tattcgaatg aagacaaaag
     2581 ttcattacat agatataaag cagatgaatc ctatttagtt ggaagtgatt taggtcctgc
     2641 tgaaagttat ttaaatattg agcgtatcat tgatgtagca aaacaagcga atgtggatgc
     2701 gattcatcct ggctatggat ttttaagtga aaatgaacaa tttgcgcgtc gttgtgcaga
     2761 agaaggaatt aaatttattg gtcctcattt agaacattta gatatgtttg gagataaagt
     2821 taaagctcgt acaacggcta tcaaggcaga tttaccagtt attcctggta cagacggtcc
     2881 aattaaatca tatgaattag caaaagaatt tgcagaagaa gctggtttcc cgctaatgat
     2941 taaagccaca agtggtggcg gcggtaaagg tatgagaatc gttcgtgaag aaagtgaatt
     3001 agaagatgct ttccatagag caaaatcaga agctgaaaaa tcatttggta atagtgaagt
     3061 ttacatagaa agatacattg ataatccaaa gcatattgaa gtacaagtta taggtgacga
     3121 acatggaaat atcgtacact tatttgaacg tgattgttca gtacaacgtc gtcatcaaaa
     3181 agttgtagaa gttgcaccat cagttggatt atcaccaaca ttacgtcaac gtatttgtga
     3241 tgctgcaatt caattgatgg aaaatattaa atatgtcaat gcaggtactg ttgaatttct
     3301 agtatctggt gacgaattct tctttataga agttaaccct cgtgtacaag ttgagcatac
     3361 aattacagag atggtaacag gaattgatat tgttaagaca caaattttag ttgcagcagg
     3421 tgccgattta tttggtgaag aaattaatat gccgcaacaa aaagatatta caacattggg
     3481 ctatgccatc caatgtcgta ttacaacaga agatccgtta aatgatttca tgccggatac
     3541 tggaacaatc attgcttatc gttcaagcgg tggctttggt gtacgtctcg atgctggaga
     3601 tggtttccaa ggtgctgaga tatcacctta ttatgattca ttactcgtaa aattatctac
     3661 acacgcgata tcatttaaac aagcagaaga aaaaatggta cgttcattac gagaaatgcg
     3721 tattcgtggt gttaaaacta atattccatt cttaattaat gtaatgaaga ataaaaagtt
     3781 cacaagtggt gattacacaa ctaaatttat tgaagaaaca ccagaacttt tcgacattca
     3841 gccgtctcta gatagaggta ctaaaacatt agaatatata ggtaatgtaa caattaatgg
     3901 tttcccaaat gttgagaaac gtccgaaacc agactatgaa ttagcatcaa ttccaactgt
     3961 atcttcaagt aaaatcgctt catttagtgg tacgaaacaa ttgcttgatg aagtaggtcc
     4021 aaaaggtgta gctgaatggg ttaaaaagca ggatgatgtt ttactaacag atacaacctt
     4081 tagagatgca caccaatcat tattagctac acgagttaga actaaggata tgattaatat
     4141 cgcatccaaa acagcggacg tatttaaaga tggtttctca cttgaaatgt ggggcggtgc
     4201 tacatttgat gtggcatata atttcttgaa ggaaaaccca tgggaacgac ttgaacgtct
     4261 acgtaaagct attccaaatg tattattcca aatgttgtta cgtgcttcaa acgcagttgg
     4321 ttataaaaac tatcctgata atgttattca taaattcgta caagaaagtg ctaaagcagg
     4381 catagatgtc tttagaattt tcgattcttt aaactgggta gatcaaatga aagttgccaa
     4441 tgaagcagta caagaagcgg gcaaaatctc tgaaggtact atttgttata caggtgacat
     4501 tttaaatcct gagcgatcaa acatttatac tttagagtat tatgtcaaac tagctaaaga
     4561 gttagaacgt gaaggtttcc atattttagc gattaaagat atggcaggct tattaaaacc
     4621 taaagccgct tacgaattga ttggtgagtt aaaatcagct gtagatttac caattcatct
     4681 tcacactcat gatacaagtg gtaatggttt attaacatac aaacaagcaa tagatgctgg
     4741 tgtcgatatc attgatactg ctgttgcttc aatgagtggt ttaacaagtc agccaagcgc
     4801 caattcgtta tattatgcat taaatggctt cccacgccac cttagaactg atattgaagg
     4861 tatggagtca cttagtcatt attggtcaac tgtacgtact tattattcag actttgaaag
     4921 tgatatcaaa tcaccgaata ctgaaattta tcaacatgaa atgcctggtg gacagtattc
     4981 gaatttaagt caacaagcta aaagtttagg tttaggcgaa agatttgatg aagtcaaaga
     5041 tatgtatcgc agagtgaatt tcttatttgg tgatatcgta aaagtaactc catcgtctaa
     5101 agtagttggt gatatggcac tttatatggt acaaaatgat cttgatgaac aatccgtgat
     5161 tacagatggc tataaattag atttcccaga atcagtagtg tcgttcttca aaggcgaaat
     5221 aggacaacct gtaaatggtt ttaataaaga tttacaagcg gttattttaa aaggccaaga
     5281 agcactaaca gctcgtccag gtgaatatct agagccagtt gattttgaaa aagtccgtga
     5341 gttgcttgaa gaagagcaac aaggtcctgt tacggagcaa gatattatta gttatgtatt
     5401 atatccgaaa gtatatgaac aatatattca aactagaaat caatacggaa acttatcgtt
     5461 acttgatacg cctacattct tctttggtat gcgtaatggt gaaacagtag aaatcgaaat
     5521 cgataagggt aaacgattaa ttattaaact agaaacgatt agtgaaccag atgaaaatgg
     5581 taatagaacg atttactatg cgatgaatgg tcaagcgaga cgtatttaca ttaaagatga
     5641 gaatgtgcat acaaatgcga acgttaagcc aaaagcagat aagagtaatc caagtcatat
     5701 cggtgcgcaa atgccaggtt cagtaactga agtcaaggtt agtgtaggtg aatctgtgaa
     5761 agctaaccag ccgttgctaa ttactgaagc tatgaaaatg gaaacaacaa ttcaagcacc
     5821 atttgacggt gtgattaaac aagtaactgt aaataatggt gacacaatag cgacaggcga
     5881 tttattaatc gaaattgaaa aagcaactgt ctaaaatgat taaaataaaa cgagattaca
     5941 caacaattga cgtgtaatct cgtttttaca tggcttaggt ttaattttaa atattgatat
     6001 a
//
LOCUS       DAHHYJ010000149         5935 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000149 DAHHYJ010000000
VERSION     DAHHYJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.149"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..983
                     /gene="argF"
                     /locus_tag="NBK22_002272"
     CDS             <1..983
                     /gene="argF"
                     /locus_tag="NBK22_002272"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD2415445.1"
                     /translation="SFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKNIALL
                     FEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYDGIEYRGF
                     SQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTYVGDGRNN
                     VANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDIDQGVKGS
                     DVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFHNADTK
                     IGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1006..1938
                     /gene="arcC"
                     /locus_tag="NBK22_002273"
     CDS             1006..1938
                     /gene="arcC"
                     /locus_tag="NBK22_002273"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD2415446.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVDENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLKTTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2110..3666
                     /locus_tag="NBK22_002274"
     CDS             2110..3666
                     /locus_tag="NBK22_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCD2415447.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKNDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFTIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            3974..4201
                     /locus_tag="NBK22_002275"
     CDS             3974..4201
                     /locus_tag="NBK22_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415448.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWNLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
     gene            complement(4506..5453)
                     /locus_tag="NBK22_002276"
     CDS             complement(4506..5453)
                     /locus_tag="NBK22_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCD2415449.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMIKYFNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLLLAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKENQDKFLRNASQ"
     gene            5702..5890
                     /locus_tag="NBK22_002277"
     CDS             5702..5890
                     /locus_tag="NBK22_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD2415450.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKNERSLDNMSL
                     KNAELLYKFANSIFSNEN"
BASE COUNT         2108 a          806 c         1116 g         1905 t
ORIGIN      
        1 gaagtttttt aactttatta gacttttcac gacaagaggt agaattctta ttaacactct
       61 ccgaggattt aaaacgtgct aaatatattg gcactgaaaa gcctatgtta aaaaataaaa
      121 atattgcact gttatttgaa aaagattcta caagaacgcg atgtgcattt gaagttgcag
      181 cgcatgatca aggtgcaaat gtaacttatt taggcccaac tggatcacaa atgggtaaaa
      241 aagaaacaac taaagatact gcacgtgtgc ttggtggaat gtatgatggc attgaatatc
      301 gtggtttttc acaaagaaca gtagaaactt tagctgaata ttcaggcgta ccagtgtgga
      361 atggtttaac tgatgaagat catcctactc aagttcttgc tgatttctta acagcaaaag
      421 aagtcttaaa aaaagattat gcagatatta actttacata tgttggagat ggtcgtaata
      481 acgttgcaaa tgcattaatg caaggcgctg ccattatggg tatgaacttc catttagttt
      541 gtccaaaaga attaaatcca acagatgaat tattaaatcg ctgtaaaaat attgccgctg
      601 aaaatggtgg caacatatta atcacagatg atattgacca aggtgtaaaa ggttcggatg
      661 taatttacac tgatgtttgg gtatcaatgg gtgaacctga tgaagtatgg aaagaacgac
      721 ttgaattatt gaaaccatat caagtaaata aagaaatgat ggataaaact ggtaatccaa
      781 atgttatttt tgagcattgc ttaccatctt tccataatgc tgatacgaaa attggtcaac
      841 aaatttttga aaaatatgga attcgagaaa tggaagttac agatgaagta ttcgaaagta
      901 aagcttcagt tgtattccaa gaagctgaga acagaatgca tacaatcaaa gcagtgatgg
      961 ttgctacatt gggtgaattt taaatgatat aaggaagtga atatgatggc gaaaatagta
     1021 gtagcattag gtggtaatgc tttaggaaaa tcacctcaag aacaactcga gcttgttaaa
     1081 aatactgcga aatcattagt aggattaata acaaaaggac atgagattgt tattagtcat
     1141 ggtaatggac cacaggttgg aagcattaat ttgggactta actatgctgc agaacataac
     1201 caaggtccgg catttccatt tgctgaatgt ggcgcaatga gtcaagctta catcggctat
     1261 caattacaag aaagcttaca aaatgaattg cattctattg gaatggataa acaagtggta
     1321 acactagtga cacaagttga agttgatgaa aatgatccgg catttaacaa tccttcaaaa
     1381 ccaattgggt tattttacaa caaagaagaa gctgaacaaa ttcaaaaaga aaaaggattt
     1441 atatttgttg aagatgctgg aagaggatat agacgcgttg ttccttcacc acaacccatc
     1501 tctattattg aattagagag tattaaaaca cttattaaaa atgatacact cgttattgct
     1561 gctggtggtg gaggtatacc agtaattaga gagcaacatg atggttttaa aggtattgat
     1621 gcagttatag acaaagataa aacaagtgcg ttgttgggtg ctaatattca atgcgatcaa
     1681 ttgattattt taacagcaat tgattatgta tatattaatt ttaacactga aaaccaacag
     1741 cctttgaaaa caacaaatgt tgatgaatta aaacgatata tagacgaaaa tcaatttgca
     1801 aaaggaagta tgttaccaaa aattgaagca gccatatcat ttattgaaaa caatccaaaa
     1861 ggaagtgtgc ttataacatc attaaatgaa ttagatgctg ccttagaggg taaagtaggt
     1921 actgtgatta aaaagtaatt gaattgaaac gcttttcaat tactatatgt caaatgcatg
     1981 atttttatta tttatgtgca ccccctgaaa ataatgcctc tattttgatg cggggtgcac
     2041 tttcttaatt tatatttata aaatctttaa ggtagaaatg ctaggttaaa tgtcgaagga
     2101 gatgaaaccg tggaaaatac aattaatgaa agtgaaaaga aaaaacgatt taaattaaaa
     2161 atgccaggtg catttatgat tttattcatt ttaacggttg ttgcagttat agcaacatgg
     2221 gttattcctg ctggtgcata ttctaaactt tcttacgaac cttcatccca agaactaaag
     2281 atagttaacc ctcataacca agtgaaaaag gttccgggta cgcaacagga actagacaaa
     2341 atgggggtta aaattaagat tgaacaattt aaatcaggtg caattaataa gccggtatca
     2401 attccgaata cttatgaaag attaaagcaa catccagctg gaccagaaca aataacaagt
     2461 agcatggttg aaggtacgat agaagcggtc gatatcatgg tattcattct tgtactaggg
     2521 ggacttattg gcgtagttca agccagtggt tcttttgaat cgggattgtt agctttaacg
     2581 aagaaaacaa aagggcatga atttatgcta attgtgtttg tatcaatact aatgattatc
     2641 ggcgggacat tatgtggtat tgaagaagaa gctgtagcat tttatccgat tttagtccct
     2701 atatttatag cgttaggata cgattctatc gtttcagttg gtgccatatt ccttgccagc
     2761 tctgtcggta gtacattttc aactattaac ccgttctcgg ttgtaattgc ctctaatgcc
     2821 gctggtacaa cttttacgga tggcttgtat tggagaatag gtgcttgtat tgtcggtgcg
     2881 atttttgtta ttagttattt atattggtac tgtaaaaaaa ttaaaaacga tcctaaagcg
     2941 tcatattctt atgaagacaa agatgctttt gaacagcaat ggtctgtatt aaaagatgac
     3001 gatagtgccc attttacttt gcgtaagaag ataatcctta cattatttgt actaccattt
     3061 ccaattatgg tatggggagt tatgacgcaa ggttggtggt tcccagttat ggcttcagca
     3121 tttttaatat ttacaattat aataatgttt attgctggga caggtaaatc tggattggga
     3181 gaaaaaggaa ctgtagatgc atttgtcaat ggtgcatcaa gtttagtagg tgtatcttta
     3241 attattggtt tagctcgagg tattaattta gtgttgaatg aaggtatgat ttcagataca
     3301 atcttacact tttcatcatc tttagttcaa catatgagtg gaccattatt tatcatcgta
     3361 ttactattta ttttcttctg tttaggtttt atcgtgccat catcttctgg cttagcggta
     3421 ttatcaatgc ctatctttgc accactagct gatacagtag gtataccaag attcgtcatc
     3481 gttacgacat atcaatttgg tcaatatgcg atgttattct tagcgccgac tggacttgtt
     3541 atggccacac tacaaatgtt aaacatgcga tattcacatt ggttccgatt tgtatggccg
     3601 gtagttgctt ttgtattgat tttcggtggc ggagtactaa ttacgcaagt actaatttat
     3661 tcataatttg aaatgctata ttataaaaaa tactaatggg gttttatgca tctcgtaggt
     3721 ttgtagaaat actaaactaa gcgaggtgca ttattatttt tgattaagaa aataatgacg
     3781 gtaatgataa cactagtaag taattgatac aatgctctat ttaataatga tatttttaaa
     3841 atttgttttt aatgtaatgt tagatctatg gtatattata tttatcgtgg taaaaatgta
     3901 tttgctgtaa acagtttttg taacacatga tatatatggt tgtgaaaata gcatatataa
     3961 aggatggcta taaatgacac atttgacaaa ggttttagat acactaactg gaatatgcgt
     4021 agtattatta tttagtaaat attttgtggc gtatgcaaat atggtgtttg attggaattt
     4081 aagatggtat ttgctagaaa acataccaca tttgccaatt atattattta ttctgatgtt
     4141 tattttcgga gtaccttctg aaatgataaa agataggcaa aggaaaaata acggcgttta
     4201 atttatcgat atttagaggt gataaaattt gtcaactatt acaaagacta aaataaaaat
     4261 ctttattaat tattttattg gtatattagc cttggcgttt gctgcttata tattatttaa
     4321 ataaaggtta gagtgaaacg tgtttatgaa ctagacgttc tagtatagtt actacagctt
     4381 agtcagaagg tatcattgat aagatcatat taaatcaaag aggcattgat atacactaaa
     4441 aagaggcaag attacctgcc tcttttttag ttattaaata tgcgtgttaa ttcttggtaa
     4501 tagtgttact gagacgcatt acgaagaaat ttatcttgat tttccttttt taaaaagaag
     4561 tgaagatatc ctataaagac tctaagtact attacagtag ctaataacac ttcgataaac
     4621 aaaagacctt tccaaatgtc tggaaacata agtacaggca aactgttctt taaagcagtt
     4681 gctgagatta ctaaagggaa tgtgaaagct gaaaatacgg gcgaaaaagg ttcttttagt
     4741 aatttaggca attgtataat gatataaaaa taaaatattt gagctaatag taaaaatata
     4801 attacgataa aagcattcgc cttaggaaag gctataacat atgctgcagc gactaaagaa
     4861 aatggtgcac aaattgtcga tgtgttcggt ttgattgacg tctgcaatgg aaatgctttt
     4921 aatcgcttga aaactatagg taagacaata caagtagcta caaagccata tattactgtt
     4981 aattgaccta tgaaaaaata tccgctaacg ggtgccgtca atcctgcgat agcaatacca
     5041 ataaaaagta cagtccacga aggataaaca ttttcaagtg aaaaatcttt taaatatttt
     5101 attgaaaaaa taatcatatg cgtcataatt cccacaaggc ataaaatcca aataggcgtt
     5161 attaagctat tgataaaagt tatgttacta aaaaatgtat ttaaataagt agtacctaaa
     5221 aagccagaca tgaaaaatgt tgtgaacact gatgaaacta gaggactgtt taattgttct
     5281 ttcacattat taaaatattt gatcatagta cataaaaggt gaatccaaat caagaaagca
     5341 aagattccgc aaacagcgtt taaagtaaga gataagtctt ttaatagatt acccaggcct
     5401 aataatccta agactagtcc tgacgttact agaggtgctt tttgaagtct catgatttac
     5461 aacctttctt atgtgatttt cttcactaat tatatcatga tagctatggc caattaataa
     5521 gaagagtgtg tactattacg ttattagagt atgtatattg gattgataga cacataattg
     5581 acatttaaat ctcaaaatta atgatatttg aggtatgctt tgcaacctaa atattggaat
     5641 atgtggaaaa gtaattattt aatttaaatg atgattgaag atttacaagg gggtgtacaa
     5701 aatgagaaat caaattcaaa aactattaga cagtgatttg agcagtttac atatatcgaa
     5761 acaaacagga gttccacaaa gcacaataca cagaatgaga aaaaatgaaa gatcattaga
     5821 caatatgtca ttgaaaaacg ctgaactact ttataaattt gccaatagta tatttagcaa
     5881 tgaaaattaa gaagaataat tagtgagtta aataattaaa aaaccacgtc tcgat
//
LOCUS       DAHHYJ010000150         5880 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000150 DAHHYJ010000000
VERSION     DAHHYJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.150"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            70..1368
                     /locus_tag="NBK22_002278"
     CDS             70..1368
                     /locus_tag="NBK22_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD2415451.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            1820..2761
                     /gene="pde2"
                     /locus_tag="NBK22_002279"
     CDS             1820..2761
                     /gene="pde2"
                     /locus_tag="NBK22_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCD2415452.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMDDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            2782..>5880
                     /locus_tag="NBK22_002280"
     CDS             2782..>5880
                     /locus_tag="NBK22_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCD2415453.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDACIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKKVGDQHRDIVQVFETHNDTYMDHLSISIQGRKHVWIQD
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTEKEVNQLDINQEYLTQVDV
                     IAQKCNAELKYHQSLLPQYETPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTNDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIEKIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKKTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSFLKVHYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHWFYKPSQKGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDHR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSQIDMFEEMIKATKENANDVMLSFY
                     DET"
BASE COUNT         2114 a          811 c         1122 g         1833 t
ORIGIN      
        1 acttcaatgc tttttatcca aaataaagtt ataatgtagt taatactgtt cgaaaagaag
       61 aggtaaagaa tgacaaaaca tgaacaaatt ttagattata ttgaatcgtt atctataggc
      121 tctaaaattt cggtgcgtaa aatcgctaaa tttttaaacg tatctgaagg gacagcatat
      181 cgtgcaatta aagatgctga taaaatgggc atggtcgcaa cgatagatcg tgttggtacg
      241 gttcgaatcg aaaaacgtaa tcgtaatgaa attgaacatt taacttttaa tgaaattgtc
      301 aatattattg atggccaagt gttaggtggc aataaaggta tcactaagat ggtatctaaa
      361 tttgcaattg gagcaatgga actcaaagat attttaaaat atattggtcc aaagacatta
      421 ttgattgtcg gaaatcgcga agatgtgcag attgaagctt taaaaagagg tacggctata
      481 ttaattactg gtggatttaa gccatctaat aaagttatta attttgcgaa tgaacatgat
      541 ttacctgttc tatcatcaag ttatgataca ttcctagttg caaatattat caacaaagca
      601 ctttttaatc agaaaatacg aaaagatatc ttaatagttc aagatataat gacgccatta
      661 gatgatttgt ctgtgctgtt tgatacgatg aaaatagcag attataagcg tatggcgaat
      721 cgaactggcc atacaagatt tccggtagtc aatgagtcgt ataaattagt aggtattgta
      781 acaagtcgag agatgattaa tacaaaggac gacgacgaaa tagataaagt gatgacgcga
      841 aatcctattt atgtaaatgc tatgagtacg gtagcaagtt gtgcgcatat gatgatttgg
      901 gaaggtattg aattgattcc tgtcgtttcg agtaataaaa agacagtggg tgtgattaat
      961 agacaagatg ttttaaagtc tatgcaactt ttaggaagac agcctcagat gggtgaaaca
     1021 attaacgatc aaatcgccaa atatataaca atgaatcaag atggaattac agtagaagtt
     1081 tcaccacttt taatcaatca ttacggaaca gtaagtaaag cggcatttgt aagtattata
     1141 gaagaaacaa tacaatatga aatgcgtaaa tttaaaaaag gtaatgtcat gatagagaac
     1201 ttaaatatcg tgtatattaa aacagtacct attgaatcac atataacagt gcgctttggc
     1261 atactagacg tcggaagaaa cttcgccaaa atagaagtga acatgcatag tcaaaacgac
     1321 aaagtagcga gcgctctcgt gatatgccaa atgtttgacg aagtatagga acttagcaga
     1381 gaaaataggc ataagataga tttcgcacaa acgttggagc ccaacttgca cattatagta
     1441 agctgacatt acgtcacatt ctatgttggg gcccacccca acttgcatgg tctgtagaat
     1501 ttcttttcga aattctctat gttggggccc caccccaact tgcattgtct gtagaaattg
     1561 ggggtccaat ttctctatgt tggggcccct gaattaaata aagcttgtta tttgtgcatt
     1621 tttatttggt caactgttgc tagtataata ttgcttgatg tcctataatc gttatgaact
     1681 aaatagtatg aaaaaatata tctaaataat taaaacgggc aaatagttta atatggtagt
     1741 aacaattttt ataatttaga tattttgata tagactaatg cttaaattaa gcaacttttc
     1801 taaaaaaggg agtatgaaaa tgattagtac tttgaatgaa attatgaaat gtatcgaaga
     1861 caacgataca attattatac acagacatgt tagaccagat cctgatgcat atggttcaca
     1921 acttggttta aaatactata ttcagcagaa atttccgcaa aagcaagtat ttgcagtggg
     1981 tgaagcggaa tcatcattaa gttttattgg agagttagat aacattgatg ataaaacata
     2041 tcaagatgcg cttgtaattg tatgtgatac tgccaatgct ccacgaattg acgatgaacg
     2101 ttatagtaca ggtcgtaaac ttattaaaat agatcatcat cctgcagttg atcagtatgg
     2161 tgatattaat ttagttaata cgaacgcgtc atctacaagt gaaatcattt acgatttaat
     2221 ctcacatttt aatgatgaag caattgttaa taaagatatc gcgagcgttt tatatcttgg
     2281 tatcgtcggt gatactgggc gattcctttt taacaatacc tcagaacata ctatggaaat
     2341 tgctggaaag ttaattgggc atgatattga tcataatgcg cttttaaata aaatgatgga
     2401 gaaggatcca aaaatgttgc cgtttcaagg ttatgtttta caacatttcg aacttatgga
     2461 tgatggattc tgccaagtta aaataactga agatgtattg gagcaattcg gtattcagcc
     2521 aaatgaagca tctcagtttg ttaatacaat tgctgacatc aaaggtttga aaatatgggt
     2581 gtttgcagtt gatgagggta gtgaaataag atgtcgctta cgctctaaag ggcaattgat
     2641 tattaatgat attgcgcaag attttggtgg cggtggtcat ccgaatgcgt caggagtttc
     2701 agtggacagc tgggatgaat ttgagcaact tgctacagct ttacgcacaa aactaaacta
     2761 atagaaagga gccattcaat catggtggca tatttaaata ttcatacggc ttatgatttg
     2821 ttaaattcaa gcttaaaaat agaagatgcc gtaagacttg ctgtgtctga aaatgttgat
     2881 gcacttgcca taactgacac caatgtattg tatggttttc ctaaatttta tgatgcatgt
     2941 atagcaaata acattaaacc gatttttggt atgacaatat atgtgacaaa tggattaaat
     3001 acagtcgaaa cagttgttct agctaaaaat aatgatggat taaaagattt gtatcaacta
     3061 tcatcggaaa taaaaatgaa tgcattagaa catgtgtcgt ttgaattatt aaaacgattt
     3121 tctaacaata tgattatcat ttttaaaaaa gtcggtgatc aacatcgtga tattgtacaa
     3181 gtgtttgaaa cccataatga cacatatatg gaccacctta gtatttcgat tcaaggtaga
     3241 aaacatgttt ggattcaaga tgtttgttac caaacacgtc aagatgccga tacgatttct
     3301 gcattagcag ctattagaga caatacaaaa ttagacttaa ttcatgatca agaagatttt
     3361 ggtgcacatt ttttaactga aaaggaagtt aatcaattag atattaacca agaatattta
     3421 acgcaggttg atgttatagc tcaaaagtgt aatgcagaat taaaatatca tcaatctcta
     3481 cttcctcaat atgagacacc taatgatgaa tcagctaaaa aatatttgtg gcgtgtctta
     3541 gttacacaat taaaaaaatt agaacttaat tatgacgtct atttagagcg attgaaatat
     3601 gagtataaag ttattactaa tatgggtttt gaagattatt tcttaatagt aagtgattta
     3661 atccattatg cgaaaacgaa tgatgtgatg gtaggtcctg gtcgtggttc ttcagctggc
     3721 tcactggtca gttatttatt gggaattaca acgattgatc ctattaaatt caatctatta
     3781 tttgaacgtt ttttaaaccc agaacgtgta acaatgcctg atattgatat tgactttgaa
     3841 gatacacgcc gagaaagggt cattcagtac gtccaagaaa aatatggcga gctacatgta
     3901 tctggaattg tgactttcgg tcatctgctt gcaagagcag ttgctagaga tgttggaaga
     3961 attatggggt ttgatgaagt tacattaaat gaaatttcaa gtttaatccc acataaatta
     4021 ggaattacac ttgatgaagc atatcaaatt gacgatttta aaaagtttgt acatcgaaac
     4081 catcgacatg aacgctggtt cagtatttgt aaaaagttag aaggtttacc aagacataca
     4141 tctacacatg cggcaggaat tattattaat gaccatccat tatatgaata tgccccttta
     4201 acgaaagggg atacaggatt attaacgcaa tggacaatga ctgaagccga acgtattggg
     4261 ttattaaaaa tagattttct agggttgaga aacttatcga ttattcatca aatcttaaca
     4321 caagtcaaaa aagatttagg tattaatatt gatatcgaaa agattccgtt tgatgatcaa
     4381 aaagtgtttg aattgttgtc gcaaggagat acgactggca tattccaatt agagtctgac
     4441 ggtgtaagaa gtgtattaaa aaaattaaag ccggaacact ttgaagatat tgttgctgta
     4501 acttctttgt atagaccagg tccaatggaa gaaattccaa cttacattac aagaagacat
     4561 gatccaagca aagttcaata tttacatccg catttagaac ctatattaaa aaaaacttac
     4621 ggtgttatta tttatcaaga gcaaattatg caaatagcga gcacatttgc aaacttcagt
     4681 tatggtgaag cggatatttt aagaagagca atgagtaaaa aaaatagagc tgttcttgaa
     4741 agtgagcgtc aacattttat agaaggtgca aagcaaaatg gttatcacga agacattagt
     4801 aagcaaatat ttgatttgat tctgaaattt gctgattatg gttttcctag agcacatgct
     4861 gtcagctatt ctaaaattgc atacattatg agctttttaa aagtccatta tccaaattat
     4921 ttttacgcaa atattttaag taatgttatt ggaagtgaga agaaaactgc tcaaatgata
     4981 gaagaagcaa aaaaacaagg tatcactata ttgccaccga acattaacga aagtcattgg
     5041 ttttataaac cttcccaaaa aggcatttat ttatcaattg gtacaattaa aggtgttggt
     5101 tatcaaagtg tgaaagtgat tgttgatgaa cgttatcaga acggcaaatt taaagatttc
     5161 tttgattttg ctagacgtat accgaagaga gtcaaaacga gaaagttact tgaagcacta
     5221 attttagtgg gagcgtttga tgcttttggt aaaacacgtt caacgttgtt gcaagctatt
     5281 gatcaagtgt tggatggcga tttaaacatt gaacaagatg gttttttatt tgatatttta
     5341 acgccaaaac agatgtatga agataaagaa gaattgcctg atgcacttat tagtcagtac
     5401 gaaaaagaat atttaggatt ttatgtttcg caacacccag tagataaaaa gtttgttgcc
     5461 aaacaatatt taacgatatt taaattgagt aacgcgcaga ataataaacc tatattagta
     5521 cagtttgata aagttaaaca aattcgaact aaaaatggtc aaaatatggc attcgtcaca
     5581 ttaaatgatg gcattgaaac tttagatggt gtgattttcc ctaatcagtt taaaaagtat
     5641 gaagagttgt tatcacataa tgacttgttt atagttagcg ggaaatttga ccatagaaag
     5701 caacaacggc aactaattat aaatgagatt caaacattag ccacttttga agaacaaaaa
     5761 ttagcatttg ccaaacaaat tataattaga aataaatcac aaatagatat gtttgaagag
     5821 atgattaaag ctacgaaaga gaatgctaat gatgttatgt tatcctttta tgatgaaacg
//
LOCUS       DAHHYJ010000151         5860 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000151 DAHHYJ010000000
VERSION     DAHHYJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.151"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            192..1148
                     /locus_tag="NBK22_002281"
     CDS             192..1148
                     /locus_tag="NBK22_002281"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD2415454.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1348..1827
                     /locus_tag="NBK22_002282"
     CDS             1348..1827
                     /locus_tag="NBK22_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD2415455.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1842..2681
                     /gene="fakB2"
                     /locus_tag="NBK22_002283"
     CDS             1842..2681
                     /gene="fakB2"
                     /locus_tag="NBK22_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCD2415456.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2767..3300
                     /gene="msrA"
                     /locus_tag="NBK22_002284"
     CDS             2767..3300
                     /gene="msrA"
                     /locus_tag="NBK22_002284"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD2415457.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3293..3721
                     /gene="msrB"
                     /locus_tag="NBK22_002285"
     CDS             3293..3721
                     /gene="msrB"
                     /locus_tag="NBK22_002285"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD2415458.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3733..4233
                     /locus_tag="NBK22_002286"
     CDS             3733..4233
                     /locus_tag="NBK22_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2415459.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4233..4454
                     /locus_tag="NBK22_002287"
     CDS             4233..4454
                     /locus_tag="NBK22_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCD2415460.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4646..>5860
                     /locus_tag="NBK22_002288"
     CDS             4646..>5860
                     /locus_tag="NBK22_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCD2415461.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKGKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDALKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTRELKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNV"
BASE COUNT         2195 a          836 c         1060 g         1769 t
ORIGIN      
        1 ttaccaattg gaaacatata tcacgttacg atggggtagg tacttaatca gcattttata
       61 aataaagtaa cggaattcat gatattaata tcatattcct aaaatgagtg ataacaaaat
      121 gctacataaa gttaagttat atcaaactaa atatacatac tataaataat gaaaatgagg
      181 tgttatcgca tatgttgaat tcatttgatg cagcatatca cagtctttgt gaagaagttt
      241 tagaaatagg aaatacacga aatgatcgca caaatacagg tacgatttcg aaatttggtc
      301 atcaacttcg ctttgactta tctaaaggat ttccactatt aacgacaaag aaagtttctt
      361 ttaaattagt agcaaccgaa ttattatggt tcattaaagg agatacaaac atccaatact
      421 tattaaaata taataataat atatggaacg aatgggcttt tgaaaattat atcaaatcag
      481 acgagtataa aggtccagat atgacagatt tcgggcatcg tgcattgagt gatcctgaat
      541 ttaacgaaca atataaagaa caaatgaaac aatttaagca acgtattctt gaagatgata
      601 catttgcgaa gcaattcggg gatttaggaa atgtttatgg taaacaatgg cgagattggg
      661 ttgataaaga tggtaatcat tttgatcaac ttaaaacagt aattgaacaa attaagcata
      721 atccagattc aaggcgacac atcgtatctg catggaatcc aacagaaatt gatacaatgg
      781 cacttccgcc ttgtcatacc atgttccagt tttatgtcca agatggtaag ttaagttgcc
      841 agttatacca acgtagcgca gatatctttt taggtgtgcc atttaatatc gcaagctacg
      901 ctttattgac acaccttatt gccaaagaat gtggacttga agtgggtgaa tttgtgcata
      961 catttggaga tgcacatatt tattcaaatc atattgatgc gattcaaaca caattagcac
     1021 gtgaaagctt caatcctcca acattaaaaa ttaacagtga caagtctatt ttcgacataa
     1081 attatgaaga tttggaaatt gttgactatg aatcacatcc agcaataaaa gctccaatag
     1141 cagtgtagtc attgcatagt tagctaacca tatagacatc aaaatgacat catagtattt
     1201 tcaagtgcaa aaaagtactt ttttgtgtta aacgttttca taaattatgc aaaatcatta
     1261 tttctatcac actttatgat aaaaattgtg ttaaattaaa gataacttag taataaaaaa
     1321 tgaaatgata gaagaaggag gataattatg actttatcca ttctagttgc acatgacttg
     1381 caacgagtaa ttggttttga aaatcaatta ccttggcacc taccaaatga tttgaagcat
     1441 gttaaaaaat tatcaacagg tcatacttta gtaatgggtc gtaagacatt tgaatcgatt
     1501 ggtaaaccac taccgaatcg tcgaaatgtt gtacttactt cagatacaag tttcaacgta
     1561 gagggcgttg atgtaattca ctctattgaa gatatttacc aactaccggg ccatgttttc
     1621 atatttggag ggcaaacatt atttgaagaa atgattgata aagtggacga catgtatatt
     1681 actgttattg aaggtaaatt ccgtggtgat acgttctttc caccttatac atttgaagac
     1741 tgggaagttg cctcttcagt tgaaggtaaa ctagatgaga aaaatacaat tccacatacc
     1801 tttctacatt taattcgtaa aaaataaggg ggaaaacgac catgacaaaa cagattatag
     1861 taacagactc aacatccgat ttatctaaag aatacttaga agcaaacaac attcatgtaa
     1921 ttcctttaag tttaactatt gaaggagctt catacgttga ccaagtagat attacatcag
     1981 aagaatttat taatcatatt gaaaatgatg aagatgtaaa gacaagtcag ccagccatag
     2041 gtgaatttat atctgcttat gaagaactag gaaaagatgg ctctgaaatc ataagtattc
     2101 atctttcttc aggattaagt ggtacatata acactgctta ccaagcaagt caaatggtag
     2161 atgctaatgt aactgttatt gattcaaaat ctatttcttt tggtttaggg tatcaaatac
     2221 aacacctagt agagcttgta aaagaaggtg tctcaacttc tgaaatagtt aaaaagttaa
     2281 atcatttaag agaaaacatt aaattatttg tagttatagg gcaattgaat caattaatta
     2341 aaggtggcag aattagtaaa acaaaaggtt tgattggtaa tcttatgaaa attaaaccaa
     2401 ttggtacact agatgatggt cgcttagagc ttgtgcacaa tgcgagaact caaaattcta
     2461 gtatccaata cttgaaaaag gaaattgctg aatttatagg agatcatgaa atcaaatcca
     2521 ttggtgtcgc acatgctaac gtcattgaat atgttgataa attgaagaaa gtttttaatg
     2581 aagcttttca tgtgaataat tacgatataa atgtaactac accagttatt tctgcacata
     2641 ctggtcaagg tgcgattggc ctcgtagtcc ttaagaagta aatttaatct tttcagtgtt
     2701 aattacttcc atttcaatcc tttatagact aaatttataa ttagatagat agaggaggta
     2761 attcatatga caaaagaata tgcaacatta gcaggaggat gtttctggtg catggttaaa
     2821 ccatttacat catatccagg catcaagtca gtcgtatctg gttatagtgg cggtcatgtt
     2881 gacaacccaa cttatgaaca ggtatgtacg aatcaaaccg gccatgtcga agcagtacaa
     2941 attacgtttg atccagaggt tacttccttt gaaaatatat tagacatata tttcaaaaca
     3001 tttgacccaa ctgatgatca agggcaattt ttcgatagag gcgaaagcta tcaaccagtc
     3061 attttctatc atgatgaaca tcagaaaaag gctgctgagt ttaaaaagca acaattaaat
     3121 gaacaaggta ttttcaagaa accagtgatt acacctatta aaccatataa aaatttctat
     3181 ccagctgaag actaccatca agattattac aaaaagaacc cggtacatta ttaccaatat
     3241 caacgtggtt caggtagaaa agcgtttata gaatcacatt gggggaatca aaatgcttaa
     3301 aaaagataaa agtgaactaa cagatataga atatattgtt acacaagaaa acggcactga
     3361 accaccattt atgaatgaat attggaatca ttttgctaaa ggaatttatg tagataaaat
     3421 ttctggtaaa cctttattta catctgaaga aaagtttcat tctgaatgtg gatggcctag
     3481 cttttccaaa gcgcttgatg acgatgaaat tatagaatta gtcgacaaat catttggtat
     3541 gttgagaact gaagtgcgtt cagaagaatc aaatagtcat ttaggacatg tctttaatga
     3601 tggacctaaa gaaagtggcg gcttaagata ctgtatcaat tccgctgcaa ttcaatttat
     3661 tccatatgaa aagttagaag aattgggtta tggcgattta atatcacatt ttgataagta
     3721 ggagtggaaa gtatgtttaa aaaattattc ggaaaaggca aagaagttca gaaagatatc
     3781 gcaatttatg caccattaac tggagaattc gtgaaaattg aagatattcc agatcctgta
     3841 ttcgcacaaa aaatgatggg cgaaggtttt ggtattaatc caactgaagg agaagttgtg
     3901 tctccaattg caggacgtgt tgacaatgtc tttccaacta agcatgcgat tgggttaaaa
     3961 gcagataacg gattagaatt attagttcat atcggtttag acacagttca attagacggt
     4021 gaaggatttg aagtgttagt gtctagtggt gacgaagtta acgtcggaga tccattagta
     4081 agattcaacc ttgaatatat taataataac gctaaatctg taatttcacc aattataatt
     4141 actaacactg atcaagcagc ttcaattaat atttatgatg aaaatgctgt gattaaaggt
     4201 gaaacaaaag tgattgatgt gacaatgaac taatgaaaaa ctattcgttt tatcaatttg
     4261 tcatgacagt tcgtggtcga cacgacgata aaggtcgtct agcagaagag atatttgacg
     4321 atcttgcttt cccaaaacac gatgatgatt ttaacatact gtctgattat attgagacac
     4381 atggtgattt cacattgcca atgtctgtat ttgatgattt atatgaagaa tatacggaat
     4441 ggctaaaatt ttaatataat ttttaataat aatagttaga accagggtga tgcaattcgt
     4501 tatcctggtt ttaatttaaa ataaactaag ttgtgactaa aaattaatca attatagtga
     4561 aatatggtgc gctatcttgc ataaattgat atgattaact acacagaatt taaaagtaca
     4621 taatacataa taaggaagtg atacaatgga tgataagcaa cacacatctt catccgatga
     4681 tgaacgcgct gaaattgcaa caagcaatca agaccaagaa actaattcat cgaaacgcgt
     4741 tcacttaaaa cgttggcaat tcatatcaat attaattggt acaatcctaa taacagctgt
     4801 catcacagtt gttgcatata tttttataaa tcaaaaaata agtggtttaa acaaaactga
     4861 tcaagcaaac ttaaataaaa ttgaaaatgt gtataaaatc ttaaatagtg attattacaa
     4921 aaaacaggac tctgacaagt taagtaaagc tgcaattgat ggcatggtca aagaattaaa
     4981 agatccttat tctgaatatt taacaaaaga acaaacgaaa tcctttaatg aaggtgtttc
     5041 aggtgatttt gtaggtattg gtgcagaaat gcaaaagaaa aatgatcaaa ttatggttac
     5101 tagtcctatg aagggatctc cagcagaacg tgctggcatt cgtcctaaag atgtcattac
     5161 taaagtaaat ggaaaatcaa ttaaaggtaa agcattagat gaagttgtca aagatgttcg
     5221 tggtaaagaa aacactgaag tcactttaac tgttcaacga ggtagtgaag aaaaagacgt
     5281 taagattaaa cgtgaaaaaa ttcatgttaa aagtgttgag tataagaaaa aaggtaaagt
     5341 tggagttatt actattaata aattccagaa tgatacatca ggtgaattga aagatgcagt
     5401 tctaaaagct cacaaagatg gtttgaaaaa gattgtttta gatttaagaa ataatccagg
     5461 tggactacta gatgaagctg ttaaaatggc aaatattttt atcgataaag gaaaaactgt
     5521 tgttaaacta gaaaaaggta aagatactga agcaattcaa acttctaatg atgcgttaaa
     5581 agaagcgaaa gacatggata tatccatctt agtgaatgaa ggttcagcta gcgcttctga
     5641 agtctttact ggtgcgctaa aagactataa taaagctaaa gtttatgggt caaaaacatt
     5701 cggcaaaggt gtcgtacaaa ctacaagaga gcttaaggat ggttcattgt taaaatatac
     5761 tgaaatgaaa tggttaacgc cagatggtca ttatattcac ggtaaaggca ttaaaccaga
     5821 cgttactatt gacacaccta aatatcaatc tttaaatgtc
//
LOCUS       DAHHYJ010000152         5532 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000152 DAHHYJ010000000
VERSION     DAHHYJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.152"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            108..668
                     /locus_tag="NBK22_002289"
     CDS             108..668
                     /locus_tag="NBK22_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD2415462.1"
                     /translation="MNKVEAIKFNDDIVKMYEALKIKSERDYLFFKLAIHSGLKVSEL
                     LTITVFQVKRLIEKCTLSEMCKAHFHSLIKIRLPETLSKELLQYIEDRSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQASIEAGIDNVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            687..3089
                     /gene="mnhA2"
                     /locus_tag="NBK22_002290"
     CDS             687..3089
                     /gene="mnhA2"
                     /locus_tag="NBK22_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCD2415463.1"
                     /translation="MSLVYLLIAILVIMAMILLMSKRRALAKYAGYIALVAPVISSIY
                     FLIQIPSVAKLQYLSTSIPWIKTLDINLDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYFYLTLFMFSMIGIVLSDNTILMYIFWELTSVSSFLLISYWYNNGDSQ
                     FGAMQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHGLFIPMIFMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQDAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMLQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMIAIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNSNKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            3076..3501
                     /gene="mnhB2"
                     /locus_tag="NBK22_002291"
     CDS             3076..3501
                     /gene="mnhB2"
                     /locus_tag="NBK22_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCD2415464.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWI
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            3498..3842
                     /gene="mnhC2"
                     /locus_tag="NBK22_002292"
     CDS             3498..3842
                     /gene="mnhC2"
                     /locus_tag="NBK22_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCD2415465.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            3832..5328
                     /gene="mnhD2"
                     /locus_tag="NBK22_002293"
     CDS             3832..5328
                     /gene="mnhD2"
                     /locus_tag="NBK22_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCD2415466.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMASITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFVIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            5329..>5532
                     /gene="mnhE2"
                     /locus_tag="NBK22_002294"
     CDS             5329..>5532
                     /gene="mnhE2"
                     /locus_tag="NBK22_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCD2415467.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQ"
BASE COUNT         1741 a          762 c          985 g         2044 t
ORIGIN      
        1 taattttatt ttctatgttt aaaatctata aaaaaggcaa tagatatatg taactaaaat
       61 ataatatagc tatgattaag acacttaaag caaggggagg ttgagtaatg aataaagtag
      121 aagcgattaa atttaatgat gatattgtta aaatgtatga agcgctcaag ataaaatctg
      181 aacgtgacta tttattcttt aagttagcta tacatagtgg attgaaagta tcagaattat
      241 taacaattac agtctttcaa gttaagagac taattgaaaa gtgtacgtta tcagaaatgt
      301 gtaaagcaca ttttcattcg ttgattaaaa ttaggttacc agaaacatta tcgaaagaac
      361 tacttcaata tatagaggac aggagtcttt cgaatgaaga cgttcttttt caatcactac
      421 gaacaaatca agtattatct agacagcaag catatcgaat aattcaccaa gcatcaattg
      481 aagctggtat agataatgta ggactaacga cattgcgtaa gacatttgca tatcatgctt
      541 atcaaaaagg tatacctata ccagtcattc aaaagtattt agggcatcaa tctgctattg
      601 aaacactaaa ttttatcggt ttagaaaatg agtgtgaaca tagtatttat atttcattac
      661 aattatagaa acaaaggagg ctaataatga gtttggttta tttattaatt gctatacttg
      721 tgattatggc gatgatactt ctaatgtcta aacgtagagc attggctaaa tatgccgggt
      781 acatagcgtt ggttgcacct gtaatttcat ctatctattt tttgattcaa ataccatcag
      841 tagctaaact gcaatatctt tctacctcta ttccatggat taagacatta gatattaatt
      901 tagatttacg tttagatggt ttaagtttaa tgttttctct tattatttca cttattggaa
      961 ttgcagtatt cttctatgca actcaatatt tatcctctcg aaaagacaat ttaccaaggt
     1021 tttattttta tttaacgtta tttatgttca gtatgattgg tattgtatta tcagacaata
     1081 cgatattgat gtacattttt tgggaattaa cgagtgtatc atcattttta ttgatttcat
     1141 attggtataa caacggagat agtcaatttg gtgcgatgca atcatttatg attacagtat
     1201 ttggtggttt ggcattatta gttggtttta tcatgttgta tattatgaca ggaacgaata
     1261 acatcacaga gatattagga caagcagatc atattaagaa tcatggattg tttatcccta
     1321 tgatttttat gtttttatta ggtgcattta caaaatcagc acaatttcca tttcatattt
     1381 ggctacctag agcaatggct gcacctacac ctgtaagtgc ttatttacat tcagccacga
     1441 tggtaaaagc tggtatcttt ttattacttc gatttacacc attattaggt cttagcaata
     1501 tgtacatata tatcgtcacg tttgttggtt taataacaat gttatttggt tcaatcacag
     1561 ctttaaaaca atgggattta aaaggaatcc tagcatattc aacaatcagt caactaggaa
     1621 tgattatggc aatggtcggt atcggtggtg gatatgctca acaccaacaa gacgcaatag
     1681 catctattta tgtatttgta ttatttggtg cgctatttca tctaatgaat catgccatct
     1741 ttaaatgtgc gcttttcatg ggagtaggta ttttagatca tgaagcaggt tcaagggata
     1801 tacgaatttt aagtggaatg cttcaactat ttcctaaaat gaatctagtc atgacgatag
     1861 cggctctatc tatggctgga gtaccatttt taaatggatt tttaagtaaa gaaatgtttt
     1921 tagatgcatt aacacaaact ggacaattat cccaatttag tttgatttca atgatagcta
     1981 tcgtgtttgt tggtgttatt gcgagtgttt ttacattcac atatgcacta tacatggtaa
     2041 aagaagtatt ttggacaaaa tatgattcta aggtttttac taaaaaaaat atccacgaac
     2101 catggttgtt tagtttacca tctcttatat taatggtgct agtacctgta atcttttttg
     2161 taccaaatat atttgggaag gggattatcg ttccagcatt aagagctgta tcaggtggta
     2221 atcatcaaat tgatcaattg gcaccacatg tttcgcaatg gcatggattt aacataccgc
     2281 ttcttttaac catcatcatt attttattgg gtagtgtact agcaatcaaa gtagattgga
     2341 aaaaagtgtt cacaggtaaa attagacaga tttcagtttc aaaaagctat gagatggtat
     2401 atcgacattt tgaaaagttt gctacgaagc gatttaaacg tgttatgcaa gatcgtttaa
     2461 accaatacat tattatgacc ttaggcatat ttatgattat cattggatat ggttatattc
     2521 gaattggact tcctaaagta catcagttac atgtttctga atttggggca ttagaaatta
     2581 tattagcaat cgtaactgtc acaattggta tttctttaat ttttatacgt caacgactga
     2641 caatggtcat tttaaatgga gtcatcggat ttgttgtgac cttattcttt atagcaatga
     2701 aagcccctga tttagcattg actcagctag tagttgaaac aataacgacg atactattta
     2761 ttgtcagttt ttcaagatta ccaaacgtgc caagatctaa ctctaacaaa aaaagagaaa
     2821 taattaaaat ttctgtatca ctcttgatgg cacttattgt tgtatcatta atttttatta
     2881 cacaacaaac agatggttta tcatcaatat cagactttta tttaaaagct gacaaactaa
     2941 caggtggtaa aaatattgta aatgcgatac ttggtgactt tagagcatta gatacattat
     3001 ttgaaggatt agtgttaatt attactgggc taggtattta cacattatta aattatcaag
     3061 atcggagggg acaagatgaa agagaatgat gtcgtgttaa gaacggtcac gaaacttgtt
     3121 gtatttattt tattgacttt cggattctat gtcttcttcg caggtcataa taatcctggt
     3181 ggtgggttta ttggtggttt aatatttagt tcagcgttta ttttaatgtt tctggctttt
     3241 aatgttgaag aggttttaga aagtttaccg attgatttta gaattttaat gattattgga
     3301 gcattggtat catctattac tgcgataata cctatgtttt ttggaaaacc atttttgtct
     3361 caatatgaaa caacttggat acttccaatt ttaggacaaa ttcatgtaag tacaataaca
     3421 ctttttgaat taggtatttt attctcagtt gttggtgtta ttgtcacagt gatgttgtcg
     3481 cttagcggag gtcgatcatg aatttaatat tattactagt tataggattt ttagtgttta
     3541 taggaacata tatgatttta tcaatcaatt taattcgtat tgtaatcgga atttcaatat
     3601 atactcatgc tggtaatctc attattatga gtatgggaac gtatggttct agtagatcag
     3661 aaccactaat aactggtgga aaccaattgt ttgttgatcc cttgttacaa gctattgtac
     3721 taactgcaat agttataggg tttggtatga ctgcgttttt acttgtactt gtttatagaa
     3781 cttataaagt aacaaaagaa gatgaaattg aaggcctaag gggggaagat gatgctaagt
     3841 aacttattga ttttaccaat gttattacca ttcctttgtg ccttaatcct tgtattttta
     3901 aaaaataatg atcgtatttc taaatattta tacttaggta caatgactat caccacaatt
     3961 atttcattaa tgctattaat ttatgttcag cgtcaccgtc caattacgct agactttgga
     4021 ggatggtcag cgccctttgg tatacagttt ttaggagatt ctttaagttt aattatggtt
     4081 acaaccgctt cgtttgtgat tactttaatt atggcatacg gatttgggcg tggcgaacat
     4141 aaagcaaatc gttatcactt gccatcgttc atattatttt taagtgttgg cgtgataggc
     4201 tcttttctaa catcagattt atttaattta tacgtcatgt ttgaaattat gttactagcg
     4261 tcatttgtac tcattacact tggacaatct gtagaacaat tacgtgctgc aattatttat
     4321 gttgtcttga atattattgg ttcatggcta ttcttattag gtataggttt actttataaa
     4381 acagtaggta cattaaactt ttcacatatt gcaatgcgtt tgaatgacat gggagataat
     4441 cgcactgtta caatgatttc attaatcttc ttagtcgcat ttagtgcgaa agcagcgctg
     4501 gttcttttta tgtggctacc caaagcatac gcagtgttaa atactgagct tgcagcatta
     4561 tttgcagcgt taatgaccaa agtaggggcc tatgcattaa ttcgattctt cactttacta
     4621 tttgatcaac ataatgatct catacatcca ttgctagcaa ctatggcttc tataactatg
     4681 gtcatcggcg ctataggtgt cattgcttat aaagatatta aaaagattgc agcttaccaa
     4741 gtcataatct caataggatt tatcatttta ggtttaggaa caaacacgtt tgcaggtatt
     4801 aatggtgcaa tattttattt ggtaaatgac attgttgtaa aaacattgct attctttatt
     4861 attggtagtt tagtttacat tacaggctat cgacaatatc aatatttgaa tggcttagct
     4921 aaaaaagaac ctttatttgg agttgcgttt gttataatga tttttgctat tggcggcgtg
     4981 cctccattta gtggctttcc ggggaaagta cttattttcc aaggtgcatt gcaaaatggc
     5041 aattatattg gactagcgtt aatgattatt actagtctaa ttgcaatgta cagtttattt
     5101 aggatacttt tttatatgta ttttggagat aaagatgggg aggaagttaa ttttaagaaa
     5161 atcccgctat atcgaaaaag aattttaagt attttagtag ttgtggttat cgcaatcgga
     5221 attgctgcgc ctgttgtgtt aaatgttaca agtgatgcaa ctgagttgaa cacgagtgat
     5281 caattatatc aaaaacttgt aaatccgcat ttgaaaggag aggactaaat gaatcaaata
     5341 gttttaaata ttatcattgc attcttatgg gtattatttc aagatgaaga tcattttaaa
     5401 ttctcgactt tcttttctgg atatctaatt ggtttaattg tcatttatat attacacagg
     5461 tttttcagcg atgattttta tgttagaaaa atttgggtag ctattaaatt tttaggcgtt
     5521 tatttatatc aa
//
LOCUS       DAHHYJ010000153         5500 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000153 DAHHYJ010000000
VERSION     DAHHYJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.153"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(416..1060)
                     /locus_tag="NBK22_002295"
     CDS             complement(416..1060)
                     /locus_tag="NBK22_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCD2415468.1"
                     /translation="MSQNTNHSYYHQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLQNPIFYTI
                     ITVVSLLLFLLYTLYDFNRLKRGDYSPREMGFNLFINLLNIIKDILYLANMFRR"
     gene            1421..2218
                     /locus_tag="NBK22_002296"
     CDS             1421..2218
                     /locus_tag="NBK22_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD2415469.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
     gene            complement(2805..3812)
                     /locus_tag="NBK22_002297"
     CDS             complement(2805..3812)
                     /locus_tag="NBK22_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCD2415470.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIIWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNIF
                     F"
     gene            complement(3828..4445)
                     /locus_tag="NBK22_002298"
     CDS             complement(3828..4445)
                     /locus_tag="NBK22_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCD2415471.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
     gene            complement(5170..>5500)
                     /locus_tag="NBK22_002299"
     CDS             complement(5170..>5500)
                     /locus_tag="NBK22_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415472.1"
                     /translation="FLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMARGLKFK
                     IMFNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPVFIVMGLYICMYAVFAVTAYNHSK
                     RTIRHSI"
BASE COUNT         1976 a          919 c          709 g         1896 t
ORIGIN      
        1 agttatatat tcattttcaa tttaaaaata aacacttatt taaatcacat tctacttttg
       61 agatgagctc aaactaacat taaattgttt atattatcta tacttcttcc tattaatgtt
      121 tcgattttaa aattaatacg tagctatact ttacttttct aataaaagtg taattagaaa
      181 ggcaagtaca cctgtttaaa gtttatcttc taagatttgt ttactttaga actatagcag
      241 ttaatcattt caatgattaa taatatcttt acaattaagg taatatattt ttattgcttt
      301 atcagtaaaa tctcagttta agtttctaac ttatgacaat cgctccagtt taaggttgat
      361 attcattatt gattattcca aaactttata caaatttctt tattaattta actgtttatc
      421 ttctgaacat attagcaaga taaagtatat ccttaataat attcaacaaa ttaataaata
      481 gattaaatcc catttctctt ggtgaatagt cacctctttt taaacgatta aaatcataca
      541 aagtataaag tagaaataac aacaaactaa cgacggtaat aatagtgtaa aaaataggat
      601 tttgaagaaa aataccaatt agactcgcaa taattaatgt tattaatgta acgaataaat
      661 atttgcctat actcgatgca tctccaacga agaaataacc aataatacca aatgctataa
      721 atgcaaaaat tgctaatgcg atatttttat agaaaatatc tggtcctaaa ttttgtaaat
      781 acgtggtaaa cgttgcgtat gacaataagc cgatcactac agcgtaaata tgtgaaataa
      841 ttaaaccgaa ccgtctcgct ctttcaaaaa caagtgttgc taaaataata atcagtaatc
      901 caaatgacaa gggttgtcgc caacttaatg gtaaaaactg acctagatag catcctatgc
      961 caaatataat ccaataatac ataaaataaa gccacacttt acttattgat tgagcatgct
     1021 ggttttgatg ataatatgaa tgatttgtat tttgcgacaa aataattcct ccttctactg
     1081 tcgttttatg cgtcaaattt tacagtaaat tttgcttcat caaaagaaat aacctttaat
     1141 aagtatagta attttacatt ttacaatgtt acaaaatata attttttata attagttaaa
     1201 atcactaaaa cgcttttata cactatcaaa tcagcattta taaaaatatg aaccgatatc
     1261 ctaaaatgtt aataatatta caagataata acaaaccaca caaagctact tatttttgat
     1321 aatatggaaa tcgtaatata aaacgaaaac ttaatttact atataaattg tcttaataat
     1381 ttttaaaagt agtaaaactt aattttaagg aggagtccct ttgaaaaaat tagcatttgc
     1441 aataacagca acatctggtg cagctgcatt tttaacgcat catgatgcac aagcttctac
     1501 acaacataca gtacaatctg gtgaatcatt atggagtatt gctcaaaaat acaacacttc
     1561 agtagagagt attaaacaaa ataaccaatt agataacaac ttggtattcc ctggtcaagt
     1621 tatctcagta ggtggaagtg atgcacaaaa tacgtcaaac acttctccac aagctggttc
     1681 agcatcatct catactgtac aagctggtga atcattaaat atcattgcta gcagatatgg
     1741 tgtttcagtt gatcaattaa tggcagccaa taacttacgt ggttatttaa ttatgcctaa
     1801 ccaaacatta caaattccta atggtggatc aggtggtaca acaccaacag ctacaacagg
     1861 tagcaatggc aatgcatcat cttttaatca ccaaaattta tacactgctg gtcaatgtac
     1921 atggtacgta tttgaccgtc gtgctcaagc tggtagtcca attagcacat attggtcaga
     1981 cgctaagtat tgggctggta acgcagctaa tgatggttac caagtaaaca acacaccatc
     2041 agttggttca attatgcaaa gcacacctgg tccatatggt catgttgctt atgttgaacg
     2101 tgtcaatggt gatggtagta tcttgatttc tgaaatgaat tacacatatg gtccatacaa
     2161 tatgaactac cgtacaattc cagcttcaga agtttctagc tatgcattca tccattaatt
     2221 aaataaattg tactgatata tactagcaat tcacatcatg tgagattgct agttttttat
     2281 ttttgaaaaa aattttcatt ttggtacaaa aaattatctc acccttccct atcatacata
     2341 tttatatttt gtatgaatgg tagttaggta aaaattaaca acctacctat ttgattaaat
     2401 taaatgcata atatattaaa aatcaaaata atgcaactaa gttgactata taatgattta
     2461 atttgttata ccagtatttt acgctttttc gtctacatat acaaatttat attaaataaa
     2521 gcccaataca atttaggtta attaaacaag ttgataacta tttaattatt ccttcattga
     2581 agaatataaa ctattaaatc attattttgc tcttacatat attttaatga cctaactgat
     2641 tatgttccat ggaatacatt tataatatag ccccaaatta aaatgctttg tcttggtctt
     2701 tctatgtaaa ctatataaaa tatgttatct acttacataa aaaaatgaac ttcaatacca
     2761 ccatatgttt gtgatactga agttcagttt agttttattt tcaattagaa aaatatgtta
     2821 agtatataga atagtaaacc tgctaacaat gctgaaatag gtaatgtaat cacccatgta
     2881 atgatcattc gttgtgcagt actccatttt acacctttag ctcggttaga agcaccaaca
     2941 cctaagattg atgatgacac aacgtgagtt gttgataatg ggaaatgtag cgatgatgca
     3001 acaaaaattg ttaatgcaga tgataaatcg gccgcagcac catttgctgg acgtattttc
     3061 ataatattac cacctacagt tttgataatt ttccagccac caattgcagt accaagcccc
     3121 attgctgtcg cacaggcaaa ttttacccat aactgtggtt caacgctgcc atcattctgt
     3181 acattagcaa caattaatgc caacgtaata atacccattg acttttgcgc atcattcgta
     3241 ccgtgagaga atgattgtaa cgctgctgtg aaaatttgga aaaatctaaa gttacgattc
     3301 gctcttgtta aatttgcatt tttaaagata actttaaaaa ttgaatacat caagaaacca
     3361 acacaaaatg cgataatcgg tgaaacgatt aatacaataa taatttttgt gaaaccttgg
     3421 taatgtaaca ctccaaatga gccttcagat gcgattgctg cacccgcaat tgaacctata
     3481 agtgcatgtg aagacgaact tggaattccg taaaaccaag tagctaaatt ccaaataata
     3541 gccgcaagta ttgcagctaa cacaacaact aatccatttt ccaatttaaa tggatcgaca
     3601 atgtctttag taatggtgcc tgcaacgccc gtaaatgtta aagcacctat aaagttcatc
     3661 actgctgcca ttaaaattgc cgttttaggc gttaacgctc tagtagatac agcagtagct
     3721 actgcattgg ctgtatcatg gaatccattg ataaagtcaa atatcagcga gaaaataact
     3781 acagctatag tgacgatgat tatatatgac ataaatatat actcccctta gctatttttc
     3841 ataataatag tttcaaaatt atttgctacg atttgacatt tatcagcgat ttcttccatg
     3901 ctttcataaa tatcttttat tttaattaaa gtgattggat ctgtttcgct attgaaaata
     3961 tgtttaattg actgtcttaa aataccatca cagtttgttt caaattcttt aatattaatt
     4021 gaatgaatac gcatatgtga taatttttta tcgactaata agccgacagc aagtttcatt
     4081 tctgcaactg ctttttgaat gttatcaaca aactcagcca tatattcatc tgtgtattcg
     4141 attgaataca tttcaaacat agctgccgtt tcttcaattg catctaaaac atcatcaatt
     4201 gcatcacata atgataaaat atcttcacgt tcaattggtg tgataaatgt ttgatttaaa
     4261 tcagtaatta cttgatgtac taattcgtca ccatgtgact cataagtttt aatgttgtct
     4321 gagtatgctt ttaaatctaa atgtgtattg aaatccattt taccgaattc aatagcagca
     4381 cgatccagat tgaaaaccat ctcttctaat tgaaccataa acttatcttt tttcttacta
     4441 aacatttaaa atcctccatt taagcgattg tcaccaatca cattcagtta taatttgttt
     4501 caaattaaga caagtgaatt tacaaactaa tgatacaaat ttgttattat caatcgtcag
     4561 tataatttta gtgtactgat attaatttca aaaatgcctc acagtaaaca atttactgta
     4621 tttgccctta tatgaaaata gcacaaaatt tacaatccca caattcaatt tacaaatttt
     4681 taacaattcc gaaacttaac ttttacataa aatttaaata gtagtaggca tttgttttga
     4741 aaaagttatt gtataggcga ttttttatct atacaactct aaaaattgta aatggatata
     4801 gttgggaaat ttgcaacgat acaatattta ttacataact tttacatatt aaaatacact
     4861 tatcttgttt aaaacgatca ttgactcata tatctctcaa tgaatatact tttattttct
     4921 cacatttaat acaatttaca aagttgaatc ctgttaatct tttcaattaa aagcatcacc
     4981 aattaaatat atttcattca aaacactttc ataatcactc atacctttac ttcattatta
     5041 atatggtatt ttaacccagt attcaactat acatgctaaa aatataactt ctcttcattt
     5101 ataataaaaa gcttccagta tagttcgtga atccgaatcc actactctac tgaaagccat
     5161 ctgtatattt tatatggaat gtctaattgt tcgcttggaa tgattataag ccgtcactgc
     5221 aaaaacagca tacatacaaa tgtataatcc cattactatg aaaaccggta tttgattcgt
     5281 tgtacccatt aatttcatat atgctaatga tgtaaaatat gcatgtgata gtgcaataac
     5341 taaaggtaac ccaaaattaa acataatttt aaactttagt ccccttgcca tatctttttg
     5401 tgtaaatcca agctttctca aaatactata attctctaac tcatcttcgg tttcatctat
     5461 ttgctttatg tatataatgc aacatacagc aatcaagaat
//
LOCUS       DAHHYJ010000154         5495 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000154 DAHHYJ010000000
VERSION     DAHHYJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.154"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..248)
                     /locus_tag="NBK22_002300"
     CDS             complement(<1..248)
                     /locus_tag="NBK22_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415473.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPIL"
     gene            complement(559..738)
                     /locus_tag="NBK22_002301"
     CDS             complement(559..738)
                     /locus_tag="NBK22_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415474.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            1697..3118
                     /gene="eap"
                     /locus_tag="NBK22_002302"
                     /gene_synonym="map"
     CDS             1697..3118
                     /gene="eap"
                     /locus_tag="NBK22_002302"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCD2415475.1"
                     /translation="MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHH
                     GHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRL
                     SKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQV
                     PYTINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYT
                     VYFKNGGKRILQLNSKNYTANLVHVKDVKRIEITVKTGTKAKADRYVPYTIAVNGTST
                     PILSDLKFTGDPRVGYKDITKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGKKKVI
                     NLNSKISQLNLLYVQDIKKIDIDVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNK
                     QLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNL
                     FSAKDIKKIDIDVKQYTKSKKNK"
     gene            3129..3419
                     /locus_tag="NBK22_002303"
     CDS             3129..3419
                     /locus_tag="NBK22_002303"
                     /inference="COORDINATES: protein motif:HMM:NF015598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD2415476.1"
                     /translation="MKFPVTINKFENIVSNEFVFYNASKITINDLSIKLKSAIANDQG
                     ITKHDIELAERAVYKVYFKNGSSKYVDLKTEYKDERVFKATDIKKVDIELKF"
     gene            complement(3698..4984)
                     /locus_tag="NBK22_002304"
     CDS             complement(3698..4984)
                     /locus_tag="NBK22_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD2415477.1"
                     /translation="MNPLAQSLNEQLQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPDHNWGNYKLVFNTRNGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQN
                     IQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR"
     gene            5184..5282
                     /locus_tag="NBK22_002305"
     CDS             5184..5282
                     /locus_tag="NBK22_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415478.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
BASE COUNT         2110 a          743 c          814 g         1828 t
ORIGIN      
        1 agtataggca aacaataaag tattgaaaaa agtaagttta atatgaaaat tgttaaatga
       61 acgacatctt ttgtttttat aaatatcaag aaaataatca aactcaaaat aaataacgta
      121 actgtagtca taggcgtcca tacataatca gcattagtca ttaagaatgg tgcagccatt
      181 atgaaaaaat ttataatgca gatgaaatag acaattagac tataaattag gtaaataaca
      241 atacacaccc ttcataaata aataatttaa atcctatata ttttaacaaa agtaaaacac
      301 agaagtgtag aaaataaaaa atattggtaa ataaaatcaa taagtttaac caatatgttg
      361 ctcgcttcat accgtatatt gcaacaaaaa ttccgatcaa gaaaaatata gcccctatga
      421 taaaacagaa atccgatgct gaactattaa aaaatgaggt gtttagagtt agaaaatgag
      481 ttaatgagtt gactataact aataagatat taattatatt tgtatggttc ttcacatgat
      541 acctccaagt aaaaaaatct aattaataaa gtgaatgctt gatgaacaag cagttattcc
      601 aaacagaatc aataagaaaa gtagaatcaa catgctaatg ccccataaac aacccttttc
      661 actttctcta ttattaattt cttgacttct tttaaagata ttattacttt tacattcttt
      721 agttgtttta aatttcacgt ttttattact tccttttgtc taaaagttta caatgaattt
      781 ttgattataa taatatattc aaaatagtac tatctagttt gatatgtcaa gcaatattat
      841 tataaaattg gaattctgag ttgtctactc taatttatta tatttaccta taaaaataca
      901 cctcaaaaaa tagatttttc agtctagctt ttggggtgta cattccacac aaacatgtga
      961 ttattttgat gtttctatta aacttgtaat tttaaattta aagtccctaa aaagtcccta
     1021 aaattttatt ttatatgggg tattattgat aatgataaag ttataaacct tgatattatg
     1081 ctgttttact ttttgaatga taagtaattt tatgttaaaa gtctccagtt tggatacaaa
     1141 acggtcgata acatataaac gttatgacta actaacttca aatcagtatc atctttctta
     1201 gattcggctt tggcactatt gtcagtaagt gcaccaacta ataataaatt tgctaatgca
     1261 agtgttgcaa ctttttttag tgaattggat tttgtttttt ttcaccatca ttatcactcc
     1321 ttttatatag cttacaacaa aatagatgca aaattgatta actaaattta aattaattat
     1381 taaatcgaat ttatattttt aagtaaatgt tttttgagta taaagatgct gtaaatataa
     1441 ttgacaaatg taatcggaat taagaaatga agattgttat gacatttaag tttgaaactt
     1501 ggtataaaca aaatcaaaca acattttagg tattgtaaat tagttagtta atcaaaatta
     1561 aaagaacttt taacagtgtt aaaaaattat tgttaaagat aatttattat taatattcag
     1621 ttaattcaaa aaatagagaa agtctggcta taataaagtt gcaatcacga attacataaa
     1681 aaaggagaga taatttatga aatttaagtc attgattaca acaacattag cattaggcgt
     1741 tatagcatca acaggagcaa actttaatac taacgaagca tctgccgcag ctaagccatt
     1801 agataaatca tcaagtacat tacaccatgg acattctaac atccagattc catatacaat
     1861 tactgtgaac ggcacaagcc aaaacatttt atcaagctta acatttaata agaatcaaaa
     1921 tattagttat aaagatatag agaataaagt taaatcagtt ttatacttta atagaggtat
     1981 tagtgatatc gatttaagac tttcaaagca agcggaatat acggttcatt ttaaaaatgg
     2041 aacaaaaaga gttatcgatt tgaaatcagg tatctacaca gctgacttaa tcaatacaag
     2101 tgacattaaa gctatcagtg ttaacgtaga tactaaaaag caacctaaag ataaagctaa
     2161 agcaaatgtt caagtgccat atacaattaa tttaaatggt acatctacaa acattttatc
     2221 gaatctttca ttttcaaata aaccttggac aaattacaaa aatttaacta gtcaaataaa
     2281 atcagtactg aagcatgata gaggtattag tgaacaagat ttaaaatatg ctaagaaagc
     2341 ttattatact gtttatttta aaaatggtgg taaaagaatc ttacagttaa attcaaaaaa
     2401 ttacacagca aacttagttc atgtgaaaga tgttaagaga attgaaatta ctgttaaaac
     2461 aggaactaaa gcgaaagcag acagatatgt accatacaca attgcagtaa atggcacatc
     2521 aacaccaatt ttatcagatt taaaatttac aggtgaccca cgtgtaggct acaaagatat
     2581 cactaaaaaa gttaaatcag tattgaagca tgatagaggt atcggggaac gtgaattaaa
     2641 atatgcaaaa aaagcaactt acacagtaca ttttaaaaat ggaaagaaaa aagtgattaa
     2701 tttaaactca aaaattagcc aactgaatct gctttatgtt caagatatta aaaagataga
     2761 tatcgatgtt aaaacaggaa ctaaagcgaa agcagacaga tatgtaccat atacaattgc
     2821 agtaaatggc acatcaacac caattttatc aaaactaaaa atttcgaata aacaattaat
     2881 tagttacaaa tatttaaatg acaaagtgaa atctgtatta aaaagtgaaa gaggcatcag
     2941 tgatcttgac ttaaaatttg cgaaacaagc aaaatataca gtatatttca aaaatggaaa
     3001 gaaacaagta gtgaatttaa aatcagacat ctttacacct aatttattta gtgccaaaga
     3061 tattaaaaag attgatattg atgtaaaaca atacactaaa tcaaaaaaaa ataaataaat
     3121 ctaataatgt gaaattccca gtaacaataa ataaatttga aaacatagtt tcaaatgaat
     3181 ttgtgttcta taatgcaagc aaaattacaa ttaatgattt aagtataaaa cttaaatcag
     3241 caattgcaaa tgatcaaggg ataactaaac atgacataga acttgctgaa cgcgcagtgt
     3301 ataaagtgta ttttaaaaat ggttcgtcaa aatatgtaga cttaaaaact gagtataaag
     3361 atgaaagagt atttaaagca actgacatta aaaaggtaga tattgaactt aaattctaat
     3421 ctttaatttt ataataaggc atctcacaat cgtggggtgc cttttacatt tgtagagatg
     3481 tgatgcttga atgtttgaag tgatttgcca aaagttatat aaatttgtac caaataaatt
     3541 aaattttgtt aggagagatg tataaaacta attaggtata gtgtttgaca agttgctgaa
     3601 taaaatatat cttatgatac tttgaaagaa ggaataattt taaaaataaa aaaccaataa
     3661 tccgagtcat actcatcaga ttattggttg aaattaatta tcttaagtca tcaatcgctt
     3721 tagcaattga atcaaataca tgtgggatat cttctttttc tacacagcta aacgcaatac
     3781 gaatatctgt tgcattaagt gcaataacac caattgaata tttttcaatt aaatgtttac
     3841 gaagttcttc agggtcaaca tcatgaactt ttattgccat aaagtatcca gagttaaagt
     3901 cataggcttg ccaatgtgaa tgatattgat cagcatagac aacttcctta gtgacttcat
     3961 agcgctcttt taatgtttga atattttgtt cgatttcttt atcgaattgt ttattatttt
     4021 ttaatacatg cttaacagcg ctttgtgttg gaagaggtcc actagaaatg ttacttcgta
     4081 taagaccttt tactttggct tctaatactt ctttagttgt ttgatcagac gttccaaatg
     4141 tcataaaccc aacacggaat ccccatgcga aaaattcttt tgttgcacca tctaaacgaa
     4201 caggtaatat tgcatttgaa tttaaattag ataatgcagt aaataatgat tgagtataca
     4261 catcttcata gaataaacca tagtatgcgt catccacaac agctataact tttgtacctt
     4321 tatcagctaa tgctttaatt gcatcgacga tggtagtcac ttctttatgc gtaggtgtgt
     4381 aacctgtcgg attattagga taatttaaaa tcataatgac tttatcttta ttgtatgatt
     4441 gtaaagcttc tacaagtgaa tcagtagtat aatgcccgtc tttatcaaag ataggatatg
     4501 tttgaaggtt tgcaccattt ctcgtattga aaacaagttt gtaattaccc caattatgat
     4561 ctggtaacaa gatagtgtca ccttgattta caaataaatc gccaactaaa gataaaccat
     4621 gtgttaatgc attcgtaaca attggtagtg acatgttgtc gattgatagc tctggattgt
     4681 cacgcaacat tttttgttgc cataaatcac gtaattcttc gatgccttgt ggtggcgcat
     4741 aagggaatat ttcatctgga gttaaatcat taaacattgc atctaaagat gatgcaaaca
     4801 tttttccgtc tttgtttgtc gccataccta tagttgcatt atatgttgta ctctttgctt
     4861 cagcagattg agataaaatg ccttttggat aaaacatatt ttgacctaag tcagataaca
     4921 ttgcaaaggc agttgcattt gactgttgaa gttgttcatt taagctttgg gctaaaggat
     4981 tcattattgc ttaacctctc tttaaaaaaa ttgtatgcct atatattatc ttcaattata
     5041 ccaaagataa agtgttgttg gtgaattaac gaataatatg taaaatctgc tgccaaaata
     5101 cgaacatatg ttctgttttt atgttagaat aggtgtaggt catccttcag tatgatactt
     5161 tagtcgaacg gaggtgtagt gtaatgatgt ggtggcaaga agggatgatg acacttatta
     5221 caggtggctt actaattatt tttcgacttt ggttagaact gaaatggaaa aataaaaaat
     5281 gatcctacac acataaataa ccccaagtct gctggtacca gacttggggt tgtgtagtat
     5341 aattctgtgg atacacttaa ataatatcat tgaacacaat atatcgcaat caataatcat
     5401 tttgattgaa tggttttgtt gcgctatttt agttgaaagt gattagtacc ttgaactttt
     5461 gaaattcaat aacttattaa aaaaatttat atttg
//
LOCUS       DAHHYJ010000155         5457 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.155, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000155 DAHHYJ010000000
VERSION     DAHHYJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.155"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..849
                     /locus_tag="NBK22_002306"
     CDS             <1..849
                     /locus_tag="NBK22_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD2415479.1"
                     /translation="NSSKIQNKILSLAGSSTIPDLNHSDFYSISSSYPLLREQQKIGK
                     FFSKLDRQIELEEQKLELLKQQKKGYMQKIFSQELRFKDENGNDYPEWESKSIQEIFE
                     NKGGTALETEFNFDGNYKVISIGSYSINSTYNDQNIRVNKNKKTEKYILSKGDLAMVL
                     NDKTKDGKIIGRSIFIDKDNQYIYNQRTERLIPFAENDNKFLWFLMNTDLIRNKIKGM
                     MQGATQVYINYSSIKLISIQLPLLEEQQKIRGFLEVLSGITTEQLHKIDQLKERKKAF
                     LQKMFI"
     gene            1255..1950
                     /locus_tag="NBK22_002307"
     CDS             1255..1950
                     /locus_tag="NBK22_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCD2415480.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASEQSRLSVTSKDTQ
                     ELKSYYSGTGFNFQNVSGYREENKMNVIVGQQLNVISLLGSDKERFDDGKDYNNLDVF
                     VVREGSGKQADNISIGGITKTNENKYKDTVKHVNMFIYRNTSHNTTTVKSTFHNIYKE
                     EISLKELDFKLRKQLIDNHDLYKTEPKDSKIKVTMENGDYYTFELNKKLQEHRMGDVI
                     DGTKIKEINVELN"
     gene            1972..3474
                     /locus_tag="NBK22_002308"
     CDS             1972..3474
                     /locus_tag="NBK22_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCD2415481.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTSGQAEASETNQNGVNAKT
                     NTTESTSNSITNKNEMKITDSENGKNFVKLDPINPGDKKVTGTTLPNHYILLSVDGKS
                     ADSVDGENSTFITSNNEGKFEYPLKNRKIVHNQEIEVSSSSLDDLGEDEEDEEVEETS
                     TEKAGAEEESTDAKATYTTPRYEKAYKIPNEQLNKENGRHQVFIEPITEGSGIIKGHT
                     SVKGKVALSINNKFINFEESVKGGVSKEDTKASSDGIWMPIDEKGYFNFDFKTKRFDN
                     LELKEGNDISLTFAPEDDEDALKPLIFKTKVTSLEDIEKAETKYDHAKVKEVNVLKDV
                     NEELHVDEIYGSLYHTDKGKGILDKEGTKVIKGKTKFANAVVKVYSELGEAQLFPDLQ
                     VNEKGEFNFDSHGAGFRLQNGEKLNFAVVNPITGDLLSNEFVSKEIDIEETPEQKADR
                     EFDERMENTPAYYKLYGDKIVGFDTNDFPITWFYPLGEKKVERKAPKLEK"
     gene            complement(3583..3891)
                     /gene="spn"
                     /locus_tag="NBK22_002309"
     CDS             complement(3583..3891)
                     /gene="spn"
                     /locus_tag="NBK22_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCD2415482.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHDDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            4258..5040
                     /locus_tag="NBK22_002310"
                     /pseudo
     CDS             4258..5040
                     /locus_tag="NBK22_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
     gene            5088..>5457
                     /locus_tag="NBK22_002311"
     CDS             5088..>5457
                     /locus_tag="NBK22_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2415483.1"
                     /translation="MGYLKRIGMCISLLIVIIFVTSCGGGNKITGDSKETQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGMWTIYTDFAKSNKPGELDDEGMVLNLDR
                     NTRTAKGYYFVKKFYEKDKLP"
BASE COUNT         2253 a          638 c          959 g         1607 t
ORIGIN      
        1 aattcatcta agatacaaaa taaaatacta tctttagcag gatcttcgac gataccagat
       61 ttaaaccata gtgattttta tagtattagt tcttcttatc cgctgcttag agaacagcaa
      121 aaaataggta aattcttcag caaacttgac cgacaaattg aactagaaga acaaaaactt
      181 gaattactaa aacaacagaa aaaaggctat atgcagaaaa tcttctcgca agaattgcga
      241 ttcaaagatg agaatggaaa tgattatcca gagtgggaat caaaatcaat tcaagaaata
      301 tttgagaata agggtggcac tgctctagaa acagaattta attttgacgg taattataaa
      361 gttataagta taggaagtta ttctataaat agcacttata atgatcaaaa tataagagtc
      421 aataaaaata aaaaaactga aaaatatatt ttatcaaaag gcgacttagc aatggtatta
      481 aatgataaaa caaaagatgg gaaaattata ggtagaagta tatttataga taaagataat
      541 caatatattt ataatcaaag aactgaaaga ttaataccat ttgctgaaaa tgataataaa
      601 tttttatggt tcttaatgaa tacagattta attagaaata aaataaaagg tatgatgcaa
      661 ggagcaaccc aagtttatat aaattattca tctattaaat tgatatctat acaattgcca
      721 cttcttgaag aacaacagaa aataagaggt tttctagaag tcttgtctgg aataactact
      781 gaacaattgc acaagataga ccaattaaaa gagaggaaaa aggcgttttt acagaaaatg
      841 tttatttgat ttgtcgctgc aatatagttt ttattatttg tttatttcag atgtttcacc
      901 atcatattgc ataactttta caaataagaa ataaagttca atgaaatcaa atacgaacat
      961 taaatttagg cactgtgaaa gcgcagtgtc ttttttgtgt cgaaattgtg tacagaataa
     1021 gtagttaaat aaagattaag ttgagataaa gtgttattcg taaataaaag agagtagatc
     1081 gataggaatt gaatgatatt agttaactat ttattaaatt acttaataat gattaatttt
     1141 tagttaaagt aagtttaatg tgaagcacga ccgttgctca ttataatgaa tgaggattgt
     1201 tcgtattgcg taatagaata aatcaaatag actaaaaatt gggagcatag aattatgaaa
     1261 ttaaaaaata ttgctaaagc aagtttagca ctagggattt taacaacagg gatgattaca
     1321 actactgctc agccagtaaa agcaagtgag caaagcagat tatcagttac ttcaaaagat
     1381 acacaagaat taaagagtta ttatagtgga actggattta attttcaaaa tgtcagtggt
     1441 tatagagaag aaaataaaat gaatgttatt gttggacagc aacttaatgt aatttcgtta
     1501 cttggttcag acaaagaaag atttgacgac ggaaaagatt ataataatct agatgtattt
     1561 gtagtaagag agggatctgg taaacaggca gataatattt caattggtgg aattacaaaa
     1621 acaaatgaaa acaaatataa agatactgtt aaacatgtaa atatgtttat ttatagaaat
     1681 acaagccata atactactac tgtaaaatct acattccata acatatataa agaagaaata
     1741 tcgttgaagg aattagattt caaattaaga aaacagttaa ttgataatca tgatctttat
     1801 aaaacagaac ctaaagacag taaaattaaa gttacaatgg aaaatggtga ttattatact
     1861 tttgaattga ataaaaagtt acaagaacac cgcatgggtg atgtaatcga tggtactaaa
     1921 ataaaagaaa ttaatgttga attaaactaa taatattcga gggagtaaat catgagggaa
     1981 aattttaaat tacgtaaaat gaaagtcggt ttagtatcag ttgcaattac tatgttgtat
     2041 ataatgacaa gcggacaagc agaagcatca gaaactaatc aaaacggagt aaatgcaaaa
     2101 actaatacaa ctgaatcaac tagcaattca ataactaata aaaatgaaat gaaaattact
     2161 gattcagaga atggtaaaaa ttttgtaaaa cttgacccta taaatccagg tgacaaaaaa
     2221 gtcacaggta ctacactacc taatcattac attttactat cagtcgatgg aaaaagtgca
     2281 gattccgttg atggagaaaa tagtacattt ataacttcta ataatgaagg gaaatttgaa
     2341 tatccactta aaaatcgtaa aattgtccac aaccaagaaa ttgaggtatc atcatcttct
     2401 ttagatgatt taggagaaga cgaagaggat gaagaagttg aagaaacttc aactgaaaaa
     2461 gctggtgctg aagaagaaag tacagatgca aaagctacat atacaactcc tcgatatgag
     2521 aaagcgtata aaataccgaa tgaacagtta aataaagaaa atggacgtca ccaagttttt
     2581 atcgaaccta ttactgaagg ttcaggtatt attaaaggcc atacttctgt aaaaggtaaa
     2641 gttgcgctat ctattaataa taaatttatt aattttgaag agagcgttaa gggcggtgtt
     2701 agtaaagaag atactaaagc tagttcagat ggtatctgga tgcctattga tgaaaaaggc
     2761 tactttaact ttgacttcaa aacgaaacgt ttcgataatc tagagttaaa agaaggtaat
     2821 gatatctcat taacatttgc accggaagat gatgaagatg cattaaaacc tttaattttc
     2881 aaaactaaag taacaagttt ggaagatatt gagaaagcag aaacaaaata cgaccatgca
     2941 aaagtgaaag aagttaatgt tttgaaagat gttaatgaag agttacatgt agatgaaatt
     3001 tatggaagct tatatcatac agacaaagga aaaggtattc ttgataaaga aggtactaaa
     3061 gtaattaaag ggaagactaa attcgcaaac gcagtagtga aagtatattc agaactgggt
     3121 gaagcacaat tgttccctga tttacaagtt aatgaaaaag gtgaatttaa ctttgactca
     3181 catggtgctg ggtttagatt acaaaatgga gaaaagctta acttcgcagt agttaatcca
     3241 attactggtg acttgttaag taatgagttt gtttcaaaag agattgatat tgaagaaaca
     3301 cctgaacaaa aagcggatcg tgagtttgat gaaagaatgg aaaatacacc cgcttactac
     3361 aagttatatg gtgataaaat agttggtttc gatactaatg atttcccaat cacttggttt
     3421 tatccattag gtgaaaagaa agttgaacgt aaggcaccaa aattagaaaa ataattaata
     3481 aaataaaaca gcttaatgat acgatggaac tagtgagtta atcactgact tcaacgtcat
     3541 tgagctgttt tttatttgct tcgttacaaa gcattattga atttatttta cgtgttcata
     3601 ttttgaaaca tcaaagccgt cttgcttagc tttgttgata atgtctttga ttgaatgtag
     3661 tcctttatcg gcgaagtatg atcttaagtt gtcttttgta gcttggtcag cattcttatc
     3721 taataacaca tcgatataac ttaactcgtg ttctaagaag tttgcgtcat catgtagtac
     3781 gagtccattt tgagaataca ctttcgcatc tgcttgatta ccatatccaa caacgccagt
     3841 tgctaaaaca cctaccattg ccgtagctac taaaaccttt ttaaatttca tatctatcac
     3901 tcctctaaaa aatgtaactc catcataaca ctgaatatta agaaaattac gtttattaag
     3961 tcgaattaat aatttttaat aaatagttaa agtgacaaat attgctaaaa agcaattaat
     4021 ctttaataag atgattatgg attttcctac taaaaccttg atttcaaaga gggcttaaat
     4081 caaatgaaac aataataaaa aaatgacaca acataataat aagtacaaat ttaattaaga
     4141 aattaaattg attgtatatg tatattttgg taacgtaaaa gagaaatata caaaataatt
     4201 aattatttat atgaaaagag aatataaatg aagtataaaa cagagagacg tgaagcgatg
     4261 ggatatttaa aaagactttc atggtacata agcgttatga ttttaatagt tattgtagca
     4321 ggttgtggca aaagtgacga aacaaaagaa ggttccaaag aagaacaaat taaaaagagc
     4381 tttgcgaaaa cgttagatat gtacccaatt aagaatctcg aggacttata tgacaaagaa
     4441 ggatatcgag atggcgaatt taaaaaggga gataagggga cgtggacttt actcacaagt
     4501 ttttcaaaaa gtaacaaacc aggtgaaata gatgacgaag gtatggtttt attccttaat
     4561 agaaatacaa agaaggcaac aggatattat tatatgagta aagctcatga tgaatttaat
     4621 gaaaaagatc aacaaaaaaa atatcatgtt gaattaaaaa ataataagat agtacttttg
     4681 gataaagtag aagattcaaa gctcaaaaat aaaatagaaa attttaaatt ctttagttag
     4741 tacgctgatt ttagagattt taaaaactat aaaaatggaa atatatcaag tgcagataat
     4801 gtaccaagtt tcgatgcaga atatcaaata agtaatacgg ataaaaatgt aaaaaaactt
     4861 agagaggttt atccaatcac aacaaaaaaa tctccagtat taaaattaca tatagatggt
     4921 gacataaaag gaagttcaat tggatataaa aatatagaat ttaatttttc aaaagtaaaa
     4981 gacgaagaaa cagctgttag agattttgtg aattttggac catctgatgg agttagctaa
     5041 agataattag atgtagaaat aattctaaaa cagagagacg tgaaacgatg ggatatttaa
     5101 aaaggattgg aatgtgtata agcctgttga ttgtaatcat ttttgtaaca tcttgcggtg
     5161 gtggtaataa aatcactgga gattcaaaag aaacacaaat caaaaagagt tttgcgaaaa
     5221 cgttagatat gtacccaatc aaaaatcttg aagatttata cgacaaagaa ggatatcgag
     5281 atggcgaatt taaaaaaggt gacaaaggga tgtggacgat atacacagat ttcgctaaaa
     5341 gtaataaacc gggtgaattg gatgatgaag gtatggtttt aaatctggat agaaatactc
     5401 gaacggctaa gggatattat tttgttaaga aattttatga aaaggataaa ttacctg
//
LOCUS       DAHHYJ010000156         5397 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000156 DAHHYJ010000000
VERSION     DAHHYJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.156"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..1543
                     /locus_tag="NBK22_002312"
     CDS             <1..1543
                     /locus_tag="NBK22_002312"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD2415484.1"
                     /translation="KQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSE
                     KAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQ
                     DFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVN
                     LDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDK
                     LRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFDIRN
                     DDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQH
                     MVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPT
                     CILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVERIIDTYKRKETIDKYGYSA
                     TLQEIAENDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIAEIEQEINAYLKELG
                     VLKDE"
     gene            1536..2750
                     /locus_tag="NBK22_002313"
     CDS             1536..2750
                     /locus_tag="NBK22_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001806403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD2415485.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKQLGEISEQSMYGIGASATNF
                     DSKNIYIRITDIDEKSRNLNYKNLTTPDEINDKYKLKRNDILFARTGASTGKSYIHKE
                     EKDNYNYYFAGFLIKFEINQLNSPMFIYQFTLTSKFNNWVKVMSVRSGQPGINSEEYA
                     KLPLVLPSKLEQQKIGEFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDEN
                     GNDYPDWEEKKMGAITTMFSGGTPQSTNTRYYKGDIPFIRSGEISKTYTELKINEEAL
                     NNSSAKLVEVGDLLYALYGATSGEVAISKINGAINQAVLCIRTNESVEFLLNYLFFSK
                     NKILNTFIQGGQGNLSANIIKNLIIETPTIIEQRKIADFFGIIDNLIEIQERKLEFLK
                     QRKQSLLQKMFV"
     gene            3404..3850
                     /locus_tag="NBK22_002314"
     CDS             3404..3850
                     /locus_tag="NBK22_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD2415486.1"
                     /translation="MKKNYIFVIVVAILIVLIVIAYAFHKSKKEHYIETQEKRIDLYF
                     KYNLKNYHGLKITKFQKGPMGSYSAKGYINNNKKYKFQAFIGLGDDKNQYNSDFEYNE
                     EKIGELLKEKDAKDRLTVDEIIEKEHLDKNEYEAEPPLFFFSGSLD"
     gene            4537..4919
                     /locus_tag="NBK22_002315"
                     /pseudo
     CDS             4537..4919
                     /locus_tag="NBK22_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4930..5106
                     /locus_tag="NBK22_002316"
     CDS             4930..5106
                     /locus_tag="NBK22_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415487.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
BASE COUNT         2073 a          688 c          967 g         1669 t
ORIGIN      
        1 aaaacaacgt cagcaacaag ctgaattaca taaaaaatta tggtcgattg cgaatgattt
       61 aagaggaaat atggatgcga gtgaattccg taattacatt ttaggcttga ttttctatcg
      121 cttcttatct gaaaaagcag aacaagaata cgcagatgcc ttgtcaggtg aagacatcac
      181 gtatcaagaa gcatgggcag atgaagaata ccgtgaagac ttaaaagcag aattaattga
      241 tcaagtcggt tacttcattg aaccacaaga tttattcagt gcgatgattc gtgaaattga
      301 aacgcaagat ttcgatatcg aacatctggc gacggcaatt cgcaaagttg aaacatctac
      361 attaggtgaa gaaagtgaaa atgactttat cggactgttc agcgatatgg atttgagttc
      421 aacgcgttta ggtaacaatg tcaaagaacg tactgcacta atttccaaag tcatggttaa
      481 tcttgacgat ttaccatttg ttcacagtga tatggaaatt gatatgttag gagatgcata
      541 tgaattcctt atcgggcgct ttgcggcgac agcgggtaaa aaagcaggcg agttctatac
      601 accacaacaa gtatctaaga tactggcgaa gattgtcaca gacggtaaag ataaattacg
      661 tcacgtgtat gacccaacat gtggttcagg ttcattgttg ttacgtgttg gtaaagagac
      721 acaagtgtat cgttatttcg gtcaagaacg taacaatact acatacaact tagcacggat
      781 gaatatgtta ttacatgatg tgcgttatga aaattttgat atccgtaatg atgacacgtt
      841 ggaaaatcca gcctttttag gcaatacatt tgatgcggtt attgcgaacc caccgtatag
      901 tgcgaaatgg actgcagatt caaagtttga aaatgatgaa cgcttcagcg gttacggcaa
      961 acttgcgcct aagtctaaag cagactttgc ctttattcaa cacatggtac attacctaga
     1021 cgatgaaggt accatggccg ttgtactccc acatggtgta ttattccgag gtgctgcaga
     1081 aggtgtcatt cgtcgttatt taattgaaga aaagaactac ttagaagctg tgattggttt
     1141 gccagcgaat attttctatg ggacaagtat tccaacatgt atcttagtat ttaaaaaatg
     1201 ccgccaacaa gacgacaacg tattatttat cgatgcatcc aatgattttg aaaaaggaaa
     1261 aaatcaaaac catttaagcg atgcccaagt cgaacgtatt atagacacat ataagcgtaa
     1321 agaaactatt gataaatatg gctacagcgc gacattacaa gagattgctg agaatgatta
     1381 caacttaaac ataccgagat atgtcgatac attcgaagaa gaagcgccga ttgatttaga
     1441 tcaagtccaa caagatttga aaaatatcga caaagaaatc gcagaaattg aacaagaaat
     1501 caatgcatac ctgaaagaac ttggggtgtt gaaagatgag taatacacaa aagaaaaatg
     1561 tgccagagtt gaggttccca gggtttgagg gcgaatggga agaaaagcag ttaggggaaa
     1621 tatcagaaca atcaatgtat ggaataggtg catctgcaac aaattttgat tcgaaaaata
     1681 tttatataag aattactgat attgatgaaa aatcaagaaa tttgaactat aaaaacttaa
     1741 ctacacctga tgaaattaat gataaatata agctgaaaag aaatgacatt ctttttgcgc
     1801 gaactggtgc aagtacgggc aaaagttata ttcacaaaga agaaaaggat aattataatt
     1861 attatttcgc tggattttta ataaaatttg aaataaatca actaaatagt cctatgttca
     1921 tttaccaatt tactttaaca tcaaaattta acaattgggt gaaggtcatg tctgtaaggt
     1981 caggtcaacc tgggataaat agcgaagaat atgcaaaatt acctttagtt ttgcctagta
     2041 aactagaaca gcaaaagata ggcgaattct tcagcaaact cgatcgacaa attgaactag
     2101 aagaacaaaa acttgaattg cttcaacaac agaaaaaagg ctatatgcag aaaatcttct
     2161 cacaagaatt gcgatttaag gatgagaatg gaaatgatta tccagattgg gaagagaaga
     2221 aaatgggggc aatcactaca atgtttagtg gaggtactcc gcaatcaaca aacacgagat
     2281 attataaagg ggatattcca tttataagat ctggcgagat ttctaaaaca tacactgaac
     2341 tgaaaattaa tgaagaagct cttaataatt catcagctaa attagttgaa gtaggagatt
     2401 tattatatgc tctatacggt gcaactagtg gtgaagttgc aataagtaaa attaatggtg
     2461 caattaatca agctgtattg tgtattagaa caaatgaatc tgttgaattc ttattgaatt
     2521 atttattttt tagtaaaaat aaaattttaa atactttcat tcaaggtggt caaggaaatt
     2581 tatcggcaaa tattataaaa aatttaatta tagaaactcc tacaataata gaacaacgaa
     2641 aaatagctga cttttttggt attattgata acttaattga aatacaggaa cgaaaacttg
     2701 aatttttaaa acagaggaaa caatcgcttc tacaaaaaat gtttgtttaa taagtctata
     2761 caaaacctct cactaattga gttagtgagg ggctttttaa taagcttttt tattttacga
     2821 tattaatgtc taaaaaataa gaatgctatt gaagacttct agatatgttc aaaatgatat
     2881 attttcagaa atttcagtgt agtttacaat tagatatgca aaataataat taaattatta
     2941 attggtaata tccttatgat atgatggaag tgaaataatc catttatata acaatctatt
     3001 caagatatgt gaatcaattt atttacattc gtaaaaaaac acatttaaca tgagataata
     3061 tatgtagcga gttgcaaaat gtgttttaaa ttaaaccatt atatagtaat tgtcattaca
     3121 tgaaataata acaacattag gattggggta tatttttgtt ttaacaaaaa acataacatt
     3181 gcaatgcatg aaaagaacat agactaagtt ttaaaattca atttgaatca ttaacatttt
     3241 tttaaagtta atgatttaaa aatatgatag taccagcaga catacatcaa tatttaagta
     3301 ttaagactta tgatgaatta attgaaaaat aacacttgga tagaaacaaa taaaaatcag
     3361 gatcatcact attcatcttt tcaggtaaat tgaggtaatg gatatgaaaa agaattatat
     3421 atttgtgata gttgttgcaa tattaatagt actaattgtt attgcatacg cgtttcacaa
     3481 aagtaaaaaa gaacattata ttgagacaca agaaaaacga atcgatttgt attttaagta
     3541 taacctcaaa aattatcatg gtttgaaaat aacaaaattt caaaaaggtc cgatgggttc
     3601 atattctgct aaaggttata taaataataa caaaaaatat aagttccagg cgtttattgg
     3661 tttaggtgat gataaaaatc aatataatag cgactttgaa tataatgaag aaaaaatagg
     3721 ggaattattg aaagagaaag atgctaaaga ccgattaaca gtggatgaaa taattgaaaa
     3781 agaacacttg gacaaaaatg aatatgaagc cgagccaccg ttatttttct tttcaggatc
     3841 gttggattag attcggttgt ataatctaat aaaaagtgta atcaccctca aaaacattaa
     3901 ggaacatgag tatttttaaa taatcattac agaacaaaca atgtttttta tgatgatgac
     3961 aaatataata agaaaataat taagaacgaa atgtcaaata ttaggcgtaa tttttagatt
     4021 tatctatata tttatgctac cgctcaaact gttattaaaa gttaaaaaca atattttata
     4081 ttgaacttgt gacgtgaatt tctaaatcac aaccaacaat tcacaaggaa gctagtaaat
     4141 aattttgaaa atcttaaaga ttatagaaga atcatactga ctaaaaagat tttaaaagtg
     4201 attaagtatt aaaaacacga caatcttaaa tagttaaaat acacatttgt gaagcgttat
     4261 tgacactaga tgcatatttt agaaatttga aaaatatatt gattgaaatg tatttaatta
     4321 ttggtgataa attattatta tgatatattg tgtttaggga tgttgatagg gaataacttt
     4381 acaagagggg tttttacgat ggataagtat tcaaaaaaat tatctttatt attagctgct
     4441 ggtgtattat ttgttagtcc aatttcatta tcttataatt cagatgtagc tcatgctgaa
     4501 gataagttag accattctca agcaaaggta atatatttga gtaaccaaaa tttatttgat
     4561 gaacttgaga aaaaaggtta taaactggaa gatatattta caaaagaaga aataaaaaaa
     4621 tataaagctg aagaccaatt aagagcgggt aaaactcaat atgtagaaac aggtaaagat
     4681 actgcaacat tatatctttc ttctgcatat acaaaaacaa tagctgcttt aggtgcgggg
     4741 ctattagtgt tatttctgca ctaataggag gtttagttgg cgcaggtgtt ggtagtttcc
     4801 ttggatctat agctgcatcg aatattgata ctagtaaggg aatatattta aaattaaaaa
     4861 ctaaaaagaa tgcagccggg gaatacgttc tgataggaga aaaatgggga tatcagtaag
     4921 ggtgatttta tggatagatt aaaatattca cttaaagttg gtattttagc attattatta
     4981 ttttgtactt taaattattt agttccaatg caaagcaatg ctttttcaat aattatatat
     5041 tcggcaattt ttgctgtgtt acttatgctt ttagtttata tatttttagg aattttgaag
     5101 aaatgacatg aaacaaatta gcattggcta tgaagaaatc tatgaggata gaatttttca
     5161 tagccatttt tttaaaagag caagaagtaa aaaattggaa aaccgtcgat caaataatta
     5221 aaaatgaaca cttagttaaa agtgattatg aagcagaacc accgttgttc ctcttttcgg
     5281 gatcgttgga ttagattcgg ttgtacagta tgatgaaaaa tattataata aatttataaa
     5341 aaatgccatt ttattttgaa atcatgtccc tccgttacat ttagtagtgg aggggtt
//
LOCUS       DAHHYJ010000157         5368 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.157, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000157 DAHHYJ010000000
VERSION     DAHHYJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.157"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            298..975
                     /gene="recU"
                     /locus_tag="NBK22_002317"
     CDS             298..975
                     /gene="recU"
                     /locus_tag="NBK22_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCD2415488.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNTFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEVFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
     gene            972..3155
                     /locus_tag="NBK22_002318"
     CDS             972..3155
                     /locus_tag="NBK22_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD2415489.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTAEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDTKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSTHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(3670..4011)
                     /locus_tag="NBK22_002319"
     CDS             complement(3670..4011)
                     /locus_tag="NBK22_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415490.1"
                     /translation="MTNITKEVFDNLEQEIDVFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDKLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
     gene            complement(4016..4675)
                     /gene="nth"
                     /locus_tag="NBK22_002320"
     CDS             complement(4016..4675)
                     /gene="nth"
                     /locus_tag="NBK22_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCD2415491.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            complement(4665..5351)
                     /locus_tag="NBK22_002321"
     CDS             complement(4665..5351)
                     /locus_tag="NBK22_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCD2415492.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDSAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
BASE COUNT         1823 a          900 c          840 g         1805 t
ORIGIN      
        1 taaaatcatt taaatcaaat acacctctgc tgattaacaa cacatacttg tacttgcctc
       61 aaaaataaaa attactgatc atgatttgac ttttataaca aaattcaaaa atattgtaat
      121 gagtattcat ttttataagc aaaacttttc aattaattaa aattgtgtgt aaaattccaa
      181 cattaacggt aaatgtatta taatattatt gtgttaatgc tgaaatcacg ttaaaataag
      241 ctttggtgta ttgtgtcttt cgcatttata ctaatttaag tttggtgttt cactataatg
      301 ttgtgtataa taaacaacgt ttactaccaa acttgtgtgg tgaaatttat gaattatcca
      361 aatggtaaac catatcgtaa aaatagtgct atagacggag ggaaaaagac cgctgccttt
      421 agtaatattg agtatggtgg acgtggtatg tcacttgaaa aagatatcga acattcaaat
      481 acgttttatc ttaaaagcga cattgcagtt attcacaaaa agcctacgcc agtacaaata
      541 gttaatgtca actatcctaa gcggagtaaa gctgtgatta acgaagctta ttttcgtaca
      601 ccttcaacaa ctgattacaa cggcgtttat caaggttatt atattgattt tgaagcaaag
      661 gaaactaaaa acaagacgtc ctttccttta aataatattc atgaccatca agtcgaacat
      721 atgaaaaatg catatcaaca aaaaggtatt gtgtttttaa tgattcgttt taaaacgcta
      781 gatgaagttt atcttttacc ctattcaaaa ttcgaagtat tttggaagag atataaagat
      841 aatattaaaa agtctataac agttgatgaa atacgaaaaa atggttacca tattccttat
      901 cagtatcaac caagattaga ctatctaaaa gcagttgata agttgatatt agatgaaagt
      961 gaggaccgcg tatgacggaa aacaaaggat cttctcagcc taagaaaaat ggtaataatg
     1021 gtgggaaatc caactcaaaa aagaatagaa atgtgaagag aacgattatt aagattattg
     1081 gcttcatgat tattgcattt ttcgttgttc ttttactagg tatcttattg tttgcttatt
     1141 atgcttggaa agcacctgct tttaccgaag ctaaattaca agatccgatt cctgcaaaga
     1201 tatatgacaa gaacggagaa cttgttaaaa cattagataa tggccaaaga catgagcatg
     1261 taaatttaaa agacgtgccg aaatcaatga aagacgcagt acttgcaact gaagacaatc
     1321 gtttctacga acatggcgca cttgattata aacgtttatt cggtgcaatt ggtaagaact
     1381 tgactggtgg atttggttct gaaggtgcct caacattaac acaacaagtt gttaaagatg
     1441 catttttatc acaacataaa tctattggac gtaaagctca agaagcatac ttatcatatc
     1501 gtttagaaca agagtatagt aaagatgata tcttccaagt atatctaaat aaaatttact
     1561 attctgatgg cgtaacaggt attaaagctg ctgctaagta ttactttaat aaagatttaa
     1621 aagatttaaa cttagcggaa gaagcttatt tagccggttt acctcaggtt ccaaacaact
     1681 ataatattta tgatcatcca aaagctgctg aagatcgtaa aaacactgtt ttatacttaa
     1741 tgcattatca taaacgcatt acagataaac agtgggaaga tgctaagaaa atcgatttaa
     1801 aagcgaactt agtaaatcgt actgctgaag aacgtcaaaa cattgataca aatcaagatt
     1861 ctgagtataa ttcatacgtt aactttgtaa aatctgaatt aatgaataat aaagcattca
     1921 aagatgaaaa tttaggtaat gtattacaaa gtggtattaa aatttataca aacatggata
     1981 aagatgttca aaaaacatta caaaatgatg ttgataatgg tagcttctac aagaataaag
     2041 accaacaagt tggtgcaacg attcttgata gtaaaactgg tggtttagtt gctatatctg
     2101 gtggacgtga tttcaaagac gtcgttaaca gaaaccaagc aacagatcct caccctactg
     2161 gttcatcttt aaaacctttc ttagcgtatg gacctgccat tgaaaatatg aaatgggcaa
     2221 caaaccatgc gattcaagat gaatcttcat atcaagttga tggttctaca tttagaaact
     2281 atgatacgaa gagtcacggt actgtatcta tttatgatgc tttacgacaa agtttcaata
     2341 taccagcttt aaaagcttgg caatcagtta agcaaaatgc tggtaatgat gcacctaaga
     2401 aattcgctgc caaacttggc ttaaactacg aaggcgatat tggtccatct gaagtacttg
     2461 gtggttctgc ttcagaattc tcaccaacac aattagcatc agcatttgct gcaatcgcta
     2521 acggtggtac ttataacaac gctcattcaa ttcaaaaagt agttactcgt gatggtgaaa
     2581 caatcgaata cgatcatact agccataaag cgatgagtga ttacactgca tacatgttag
     2641 ctgagatgct aaaaggtaca tttaaaccat atggttctgc atatggccat ggtgtatctg
     2701 gagtaaatat gggtgctaag acaggtactg gtacttacgg tgctgaaact tattcacaat
     2761 ataatttacc tgataatgca gcgaaagacg tgtggattaa cggctttaca cctcaataca
     2821 ctatgtcagt gtggatgggc ttcagtaaag ttaaacaata tggtgaaaac tcatttgtgg
     2881 gacatagcca acaagaatat ccacagttct tatatgaaaa tgtgatgtca aaaatttcat
     2941 ctagagatgg cgaagacttt aaacgtccta gctcagtaag tggtagtatc ccatcaatca
     3001 atgtttctgg tagtcaagat aacaacacta caaatcgtag tacacacggt gggagtgaca
     3061 catcagcaaa cagcagtggt actgcacaat caaataacaa tactagatct caacaatcta
     3121 gaaacagcgg tggattaaca ggtatattca actaatccac acaacataaa atcctcagtt
     3181 ataccatatt tatggcgtag ctgagggttt tttaggttct tcatctttta ttgctatatt
     3241 agtagaaacc ttaaaatcag tcattcacat acattttgaa acatctaaat agtttttcat
     3301 attcacatct ttagctattt aaaattattg acattgcaaa agtggttgtt cccattttta
     3361 aaattatgat tatttttctc atagtcatct tagacgctgt tttaaattga aatgtttatc
     3421 aattccctgt gtacgttaat cagtgaaata tcaagatgtt tgcaacatat tttcacaatc
     3481 atagatataa gaaatccacc tacgattcga aataggtatc ttaaagcata tctcaagata
     3541 tccattatga tgtagatgga cagtttttat attaagacgt tgtaatgaca cgacacctaa
     3601 cataaataca aatatgtgtg catgaattag ttttatatat tttcaacatt aggaaatgat
     3661 caatcttgtc tatacctgct tattctttaa ccttgtttga taactagcat tcattttaat
     3721 caattcagtt ttaaaggtct tcatttgtcg atagcccata aaatgatatt tacgttcaat
     3781 catataatca tatctttctt taaaattcat tggcacaaca ggatattgat ttaatttatc
     3841 aaaatcaata ttgccagtta tgtcattaac ctgcttaaaa taatcaataa ttttactatg
     3901 gtattcatcc aagtagggct ttgcttcact agaacctaat gtcttatttt tggcaaatac
     3961 atcaatctct tgttctaaat tatcaaatac ctcttttgta atatttgtca tgttatcacg
     4021 cttctttcaa actagcttta taacgttttt gtccttctct acaatcttct aatagtggac
     4081 aaatatcgca tttaggtttt cgggctaaac agtggtatct tccaaagaaa atgagttgat
     4141 gatggctcct attccatcta tctctaggta tgacagaaca taaacggtct tctacctgtc
     4201 tcacattatc tttccaacga ttaataccta agcgtttaga aacacgttct acatgcgtat
     4261 caacagctaa tgaaggttca tcaaatgcta cactcatgac tacattagca gttttacgtc
     4321 ctacacctgc taaactttct aattccttat gtgtttgtgg tatttctcca ttaaattgat
     4381 caatcaaaga ttgacaaagt ttcttaatat tcttagcttt gttacgatac agaccgatag
     4441 aacgaatatc attcataagt tcttcatcac tgactgccaa ataatcttca ggcgttttgt
     4501 attttttaaa cagctcagtt gttactctat ttactagaac gtctgtacat tgcgctgaca
     4561 ataatacagc aatagttaat tcgaacggat tatcatgttt taattcacat tctgcatccg
     4621 gaaacatatt tgctataaca tcaatcattt ctaatgcttt tttcttactt accatcaagg
     4681 ttctccccgt ttaaccaatc aaatttaggt accgttttaa ctgtgtgcgt cattttcggt
     4741 ttattaaatt tttctcttat ttttctagaa tcgtcaattg ttttgacatt gtttttcttc
     4801 caattaagta aaatacgatc catatattta aagctaagtt tattcaaact attcgcctcg
     4861 tctaatgccg cttgtataat tgcactatcg tgtttatcaa catcaatcca ttgatttaac
     4921 gtttctattt catatggaga taacggcctt gcaaatgtat cctctaaaac tctaaataat
     4981 tgtttaaatt tttctttact attttgctct ttcgtttcca tactttgttg cttcaatata
     5041 tgacttaatt tttcgaaaaa aggatctaga ttcatatatt cggtaaatct accttcttca
     5101 tctttttgaa cttgtaattc tagcaattca cgttgtatca aattttgaat aaccattgta
     5161 atatcgcgtg gttgcatagt tgagcccttc tgaagtaatt caattgaagg ctgtttattt
     5221 gatgtttcgg aagcataaat caatttaagc aaaatgacta aatcttgctc atctaaacct
     5281 aagtcactgt aatggtctaa taattctctt cgtatcacta caggtcttgc ttttaattga
     5341 tatttatcca tgttcgtgcc ccctttta
//
LOCUS       DAHHYJ010000158         5335 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.158, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000158 DAHHYJ010000000
VERSION     DAHHYJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.158"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            77..783
                     /locus_tag="NBK22_002322"
                     /pseudo
     CDS             77..783
                     /locus_tag="NBK22_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
     gene            908..1630
                     /gene="splB"
                     /locus_tag="NBK22_002323"
     CDS             908..1630
                     /gene="splB"
                     /locus_tag="NBK22_002323"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplB"
                     /protein_id="HCD2415493.1"
                     /translation="MNKNVVIKSLAALTILTSVTGIGITLVEEVQQTAKAENNVTKVK
                     DTNIFPYTGVVAFKSATGFVVGKNTILTNKHVSKNYKVGDRITAHPNSDKGNGGIYSI
                     KKIINYPGKEDVSVIQVEERAIERGPKGFNFNDNVTPFKYAAGAKAGERIKVIGYPHP
                     YKNKYVLYESTGPVMSVEGSSIVYSAHTESGNSGSPVLNSNNELVGIHFASDVKIDDN
                     RNAYGVYFTPEIKKFIAENIDK"
     gene            1688..2407
                     /gene="splC"
                     /locus_tag="NBK22_002324"
     CDS             1688..2407
                     /gene="splC"
                     /locus_tag="NBK22_002324"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplC"
                     /protein_id="HCD2415494.1"
                     /translation="MNKNIVIKSMAALAILTSVTGINAAVVEETQQIANAEKNVTQVK
                     DTNIFPYNGVVSFKDATGFVIGKNTIITNKHVSKDYKVGDRITAHPDGDKGNGGIYKI
                     KSISDYPGDEDISVMNIEEQAVERGPKGFNFNENVQALNFAKDAKVDDKIKVIGYPLP
                     AQNSFKQFESTGTIKRIKDNILNFDAYIEPGNSGSPVLNSNNEVIGVVYGGIGKIGSE
                     YNGAVYFTPQIKDFIQKHIEQ"
     gene            2528..3244
                     /locus_tag="NBK22_002325"
     CDS             2528..3244
                     /locus_tag="NBK22_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD2415495.1"
                     /translation="MNKNIIIKSIAAMTILTSITGVGTTVVDGIQQTAKAEHNVTKIT
                     NTSISPYKGILRVGAGTGFVVGKNTILTNRHVAKDVQVGSTVLAHPNGENDTGGYYKV
                     KKVIPYTGSADLAIVQVEEDSVYPKNKKFGENTEILTLTSEVKANERIAIVGYPAPYK
                     NKHHMYQSTGTVLSINGDKLVSDAFAEGGNSGSPVFNNKNEVVGVLYSGDQIGHSKKE
                     SYSVYLTTEIKKFITDNMAK"
     gene            3402..4118
                     /gene="splE"
                     /locus_tag="NBK22_002326"
     CDS             3402..4118
                     /gene="splE"
                     /locus_tag="NBK22_002326"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplE"
                     /protein_id="HCD2415496.1"
                     /translation="MNKNLIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIK
                     NTNVAPYNGIVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKV
                     KKIVRFSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYI
                     NKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTK
                     GYGVYFSPEIKKFIADNTDK"
     gene            4269..4988
                     /gene="splF"
                     /locus_tag="NBK22_002327"
     CDS             4269..4988
                     /gene="splF"
                     /locus_tag="NBK22_002327"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease SplF"
                     /protein_id="HCD2415497.1"
                     /translation="MNKNIIIKSIAALTILTSVTGVGTTMVEGIQQTAKAEHNVKLIK
                     NTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYK
                     VTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
                     GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGEST
                     RGFAVYFSPEIKKFITDNLDK"
     gene            5057..5191
                     /locus_tag="NBK22_002328"
                     /pseudo
     CDS             5057..5191
                     /locus_tag="NBK22_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2116 a          761 c          924 g         1534 t
ORIGIN      
        1 ttttatataa aatgtatata gtaaatgtgt atctagatac attaactata aaaataaatt
       61 ttaaaggagg taaaatataa ataaaaatgt aatggttaaa agtttaactg ctttaactat
      121 tttaacatct cttggtttcg ctgaaaatat ttctgatcag tctcattcaa ttgccaaagc
      181 agaaaagaat gtcaaagaaa ttaccgatgc aactaaggca ccatacaatt cagtggtagc
      241 atttgcgggt ggtattggtg tagttgttgg taaaaataca atcgtaacta acaaacatat
      301 cgctaaaagt aatgatattt tcaaaaatag agtatcagca catcatttga gtaaaggtaa
      361 aggcggagga aactacgacg ttaaagacat tgtagaatat ctcggaaaag aagaccttgc
      421 gatagttcat gttcatgaaa caagtacaga aggtttgaat tttaataaga acgttagtta
      481 tacaaaattt gcagacggag caaaaatgaa agacagaatt tctgttattg gttatccaaa
      541 gggtgcacaa acaaaatata aaatgtttga atcgacagga acgattaatc gtatcaatgg
      601 aacatttatg gaatttgatg cgtttgcgca accagggaac tctgaatcac ctgtattgaa
      661 ttctaaacat gaactgattg gtattttata ttcaggtagt ggaaaagatg aatctgaaaa
      721 gaatttcggt gtttatttca cacacaatta aaagaattta tacaaaataa tattgaaaaa
      781 taagtatcat caattcattc gtgaagttga ttttttaaag aatggttaag aaaacggtta
      841 cataattaac taaatatact gaattatgta tctagatatc aaaataatta aaaaagagga
      901 acttaaaatg aacaaaaacg tagtcatcaa gagtttagca gcattaacaa ttttaacatc
      961 tgtaacaggt attggaataa cattggttga ggaagtacaa caaactgcca aagcagaaaa
     1021 taatgtcaca aaagttaaag atacaaatat ttttccatat actggtgtag ttgcttttaa
     1081 aagtgcaact ggatttgtag ttggaaagaa tactatttta acaaataaac atgtgtcgaa
     1141 aaattacaaa gtgggcgata gaattactgc acatccaaat agtgataaag gtaatggtgg
     1201 tatttattcg attaaaaaga ttattaatta tccaggtaaa gaagatgtat cagtcattca
     1261 agttgaagag cgtgcaatag aacgtggacc aaaaggcttt aattttaatg ataatgtaac
     1321 gccattcaaa tatgcggcag gggctaaagc tggtgagcga attaaagtga ttggttatcc
     1381 acacccatac aaaaataaat atgttttata tgagtcaact ggccctgtga tgtcagtaga
     1441 aggtagcagt attgtatatt cagcgcatac tgaaagcgga aactctggat cacctgtatt
     1501 aaacagcaac aacgaattag ttggtattca ttttgcttct gatgtaaaaa ttgatgataa
     1561 cagaaatgca tatggcgtct actttacacc agaaattaaa aaattcattg cagaaaacat
     1621 agataaataa acaaattgac tttaaacgag cgttgcaaca tatctcgaat tgtaaaggag
     1681 cttgaaaatg aataaaaata tagtcattaa aagcatggca gcattagcca ttctaacctc
     1741 agtaactgga ataaatgctg cagtcgttga agagacacaa caaatagcaa atgcagagaa
     1801 gaatgttacg caagttaaag atacaaatat ttttccatat aatggtgtcg tttcatttaa
     1861 agatgcgaca ggttttgtaa ttggaaaaaa tacaattatc accaataaac atgtatcaaa
     1921 agattataaa gttggcgata gaattactgc ccatccagac ggtgacaaag gaaatggtgg
     1981 tatatataaa attaaaagca tttctgatta tccgggtgat gaagacatct ctgtcatgaa
     2041 tattgaagaa caagcagttg aacgtggacc aaaaggcttt aattttaatg aaaatgtcca
     2101 agcattaaat tttgcgaaag atgctaaagt tgatgacaaa attaaagtta ttggttaccc
     2161 attacctgct caaaatagtt ttaaacagtt tgaatctaca ggaactataa aaagaatcaa
     2221 agacaatatt ttaaattttg atgcatacat tgaacccggg aattcaggat caccagttct
     2281 aaattctaac aatgaggtca taggtgtggt gtatggcggt attggaaaaa ttggttctga
     2341 atataatggt gccgtatact ttacgcctca aatcaaagat tttattcaaa agcacattga
     2401 acaataaaca aatttaaata tacaccatga gcatgtgttc aataatttta atgaaaaaca
     2461 tcggtcgaat ataacataaa aaaacgtcta tatcaaaagc atcatgaata aacagaggag
     2521 cacaaaaatg aataaaaata taatcatcaa aagtattgcg gcaatgacga ttttaacatc
     2581 aataactggt gtcggcacaa cagtggttga tggtattcaa caaacagcca aagccgaaca
     2641 taatgtgacg aagataacga atactagtat ttcgccatac aaaggcatat taagagtagg
     2701 tgcaggtaca ggttttgtag taggtaaaaa taccatttta acaaatagac atgtcgcgaa
     2761 agatgttcaa gtaggcagta ctgttcttgc ccacccgaat ggcgaaaatg atacaggtgg
     2821 atactataaa gtaaaaaaag ttatcccata tacaggctcg gcagatttag ctattgttca
     2881 agttgaagaa gattcagttt acccaaaaaa taaaaagttt ggtgaaaata ctgaaatcct
     2941 aacgctgaca tcagaagtta aagcgaatga aagaattgct attgttggat atccagctcc
     3001 atataaaaat aaacatcata tgtatcaatc aacaggaaca gtactatcaa ttaatggaga
     3061 taaattagta tcagatgcat ttgctgaagg tggcaattca gggtcaccag tctttaacaa
     3121 taaaaatgaa gttgtgggcg ttttatatag tggagaccaa attggtcatt ctaaaaaaga
     3181 gtcatacagc gtatatttaa cgactgaaat taagaaattc atcacagata acatggcaaa
     3241 ataaatcatc catccgtaca ttgataaatg atttttagaa attaacaaca aaatcaacaa
     3301 ttttaaacat ctctgtgatt ctatttattc gaaatgattt aaaaaataaa acttcaaaaa
     3361 cctaacctta tattaatacg aatacttaga ggagcagaaa aatgaataaa aatttaatca
     3421 tcaaaagtat tgcagcattg acgattttaa catcagtgac tggcgtcggc acaacagtgg
     3481 ttgagggtat tcaacaaacg gctaaagctg aacataatgt gaaactaatc aaaaatacta
     3541 atgtagcacc atataatggt atcgtttcga taggatctgg aacaggattc attgtcggta
     3601 aaaatacaat tgttaccaac aagcatgtcg ttgcaggtat ggaaattggc gcacatatta
     3661 ttgcgcatcc gaatggtgaa tataataatg gcggatttta taaagttaaa aaaattgtcc
     3721 gtttttcagg tcaagaagat attgccattc tacatgtgga agataaagct gttcatccaa
     3781 aaaacaggaa ttttaaagat tacacaggca ttttaaaaat agcatcagaa gcgaaagaaa
     3841 atgaacgcat ttcaattgtt ggctatccag aaccatatat aaataaattt caaatgtatg
     3901 aatcaacagg aaaagtgctg tcagttaaag gcaacatgat tattactgat gctttcgtag
     3961 aaccaggcaa ctcaggttca gctgtattta acagtaaata cgaagttgta ggtgttcact
     4021 ttggtggaaa cggtcctgga aataaaagta caaaaggata tggtgtttat ttctctcctg
     4081 aaattaagaa attcattgca gataacacag ataaataaat ccttacatag ataaatgatt
     4141 ttaaaaatta acaacaaact caacaattca aatcatctct gtgattccat ttattcgaaa
     4201 tgattaaaaa aaataaaact tcaaaaagct aacattataa ttatacaaat acttagagga
     4261 gcagaaaaat gaataaaaat ataatcatca aaagtattgc agcattgacg attttaacat
     4321 cagtgactgg tgtcggcaca acaatggttg aagggattca acaaacggcc aaagctgagc
     4381 ataatgtgaa actaatcaaa aatactaatg tagcaccata cagtggtgtt acatggatgg
     4441 gcgctggaac aggatttgta gttggaaatc atacaatcat taccaataaa catgttactt
     4501 atcacatgaa agtcggtgat gaaattaaag cgcatcctaa tggtttttat aataatggtg
     4561 gtggacttta taaagttact aagattgtag attaccctgg taaagaagat attgcggttg
     4621 tacaagttga agaaaaatca acacaaccaa aaggtagaaa attcaaagat ttcactagta
     4681 aatttaatat agcatcagaa gctaaagaaa atgaacctat atcagtcatt ggttatccaa
     4741 atcctaatgg aaataaacta caaatgtatg aatcaactgg taaagtatta tcagtgaatg
     4801 ggaatatagt gtcatcggat gcaattattc agcctggtag ctctggttca cctatattaa
     4861 atagtaaaca cgaagctatt ggtgtaatct atgcaggtaa taagccatca ggtgaaagta
     4921 caagaggatt cgctgtttat ttctctcctg aaattaagaa attcattaca gataatttag
     4981 ataaataatt aaaacttaga cattcaccca atcctgacaa aatatactat aactaacatt
     5041 tattaatata tatatattgc attatttaat atgcatcaaa gccaatcaat gattgatttt
     5101 caccaactca attgttgatt ggttttattt atgtatgaat gaacaacttt tagacatcat
     5161 tgagaatata aattattttg aaagtatttg aaagctacaa catttccata aaatttttca
     5221 attataatcg cgccactaaa actcaaaatt tccaccacca acatcaaaat tctcaacatc
     5281 gcaacataac caaatgttat aataaatcta ttacacaaag agataaatta cttat
//
LOCUS       DAHHYJ010000159         5146 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000159 DAHHYJ010000000
VERSION     DAHHYJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.159"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..1043
                     /locus_tag="NBK22_002329"
     CDS             <1..1043
                     /locus_tag="NBK22_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD2415498.1"
                     /translation="NAIFNNVNKNDRMNDNNDADAQKYTTTMKNANNTVKSVVTVENE
                     TSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNAHVVGDKENQKITFSNNKSVVGK
                     VLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLGEPILVVGNPLGVDFKGTVTEGI
                     ISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNSGGAVVNREGKLIGVVAAKISMP
                     NVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKMKNIANLNSFERQAVKLPGKVKN
                     GVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDLRFRQIIFSHKDDLKSITAKIYR
                     DGKEKEINIKLK"
     gene            1060..2418
                     /locus_tag="NBK22_002330"
     CDS             1060..2418
                     /locus_tag="NBK22_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD2415499.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFVVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            2557..4068
                     /locus_tag="NBK22_002331"
     CDS             2557..4068
                     /locus_tag="NBK22_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD2415500.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNGITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFVVKNR"
     gene            complement(4248..4336)
                     /locus_tag="NBK22_002332"
     tRNA            complement(4248..4336)
                     /locus_tag="NBK22_002332"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4299..4301),aa:Ser,seq:gct)
     gene            complement(4339..4413)
                     /locus_tag="NBK22_002333"
     tRNA            complement(4339..4413)
                     /locus_tag="NBK22_002333"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4379..4381),aa:Asn,seq:gtt)
     gene            complement(4555..5124)
                     /locus_tag="NBK22_002334"
     CDS             complement(4555..5124)
                     /locus_tag="NBK22_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCD2415501.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT         1803 a          686 c          948 g         1709 t
ORIGIN      
        1 tgaatgccat ttttaataat gtgaacaaaa atgatcgcat gaatgataat aatgatgcag
       61 atgctcaaaa atatacgaca acgatgaaaa atgccaataa cacagttaaa tcggtcgtta
      121 cagttgaaaa tgaaacatca aaagattcat cattacctaa agataaagca tctcaagacg
      181 aagtaggatc aggtgttgta tataaaaaat ctggagatac gttatatatt gttacgaatg
      241 cacacgttgt cggtgataaa gaaaatcaaa aaataacttt ctcgaataat aagagtgttg
      301 ttgggaaagt gcttggtaaa gataaatggt cagatttagc tgttgttaaa gcaacttctt
      361 cagacagttc agtgaaagag atagctattg gagattcaaa taatttagtg ttaggagagc
      421 caatattagt cgtaggtaat ccacttggtg tagactttaa aggcactgtg acagaaggta
      481 ttatttcagg tctgaacaga aatgttccta ttgatttcga taaagataat aaatatgata
      541 tgttgatgaa agctttccaa attgatgcat cagtaaatcc aggtaactcg ggtggtgctg
      601 tcgtcaatag agaaggaaaa ttaattggtg tagttgcagc taaaattagt atgccaaacg
      661 ttgaaaatat gtcatttgca atacctgtta atgaagtaca aaagattgta aaagatttag
      721 aaacaaaagg taaaattgac tatccggatg taggtgttaa aatgaagaat attgccaatc
      781 taaatagttt tgaaagacaa gcagttaaat tgccaggaaa agttaagaac ggtgttgttg
      841 tagatcaagt tgacaacaat ggtttagcag atcaatctgg tctgaaaaaa ggtgatgtaa
      901 ttactgaatt agatggcaaa cttttagaag atgatttacg ctttaggcag attatattta
      961 gtcataaaga tgacttgaaa tcaattacag cgaagattta tagagatggt aaagagaaag
     1021 aaattaatat taaactaaaa taatgttgag gtgaacaacg tgtcaatttt tagccagttt
     1081 ttaaaaagat caagccctca acaaggtatt gtattgtact atatcgtcgc aattgtcatt
     1141 gcatttttat tattaaactt accgtatgtt cataaaccag gtgtagaagt aaatccaatt
     1201 gacacattat ttgttgccgt atccggaatt agtgttactg gattgtctcc gataagtatt
     1261 gtcgatacct attctacatt tggacaatta attatcctcg tgatattaaa tattggtgga
     1321 attggcgtca tggcaattgg tacgatgtta tgggtggtac taggtaaaca tattggaatt
     1381 agagaacgtc agttaattat gttagataat aacaaaaaca caatgagtgg taccgtcaaa
     1441 ttgattattg atattgtaaa atcaatattt gtaatcgaac tcgtaggagc catgttatta
     1501 gcattttact tttatcgaga taatccagat ttaaaatatg caatcatgca aggtgttttt
     1561 gtttctattt ctgctactac caatggtgga ttagatatta caggtaagtc attaattcct
     1621 tatgcacatg attattttgt acaagcgata gttatatttt taataatttt aggatcaatc
     1681 ggcttcccag tattattaga agttaaagct tatattcaaa atagggttac taattttaga
     1741 ttttcattat ttactaaaat tacgacatca acatatttat tcctatttgt tgttggggta
     1801 ttagccattc tattatttga acataaccat gcgttcaaag gattaagttg gcatcaatcg
     1861 ttattctatt ctctgtttca atcagcgact acaagaagtg caggtcttca aacaattgat
     1921 gtgacaactc taagtgaccc tacgaatatt atcatgggta ttttaatgtt tataggatct
     1981 tcgccaagtt cggttggtgg cggtattcgt acaacaactt tcgctatttt aattttgttt
     2041 ttaattaact ttagtaataa tgccgataaa acatccatta aagtttacaa tagagaagta
     2101 cacattatgg atattcaacg ttcatttgca gtatttacaa tggcgacaat tttaacattt
     2161 ttaggaatgc taattatatc agctactgaa aatggtaagc ttacattttt acaagtattt
     2221 tttgaagtca tgtctgcatt tggaacttgt ggactatcgc ttggtgtcac aagtgatatt
     2281 agtgatattt ccaaggtcgt actaatggta ctcatgttta taggacgtgt tggcttaata
     2341 tcatttatca ttatgatagc aggacgtcga gaaccagata aattccatta tccaaaagaa
     2401 cgtattcaaa taggataata taatagcaat ctaagttaag ttaatgtaga ttttaactgg
     2461 aacttagatt gcttttttag tttgtatttt taacttattt tataagacga ttggttttga
     2521 aaatggtaaa ctagtaacaa tgagaggtgt aacatgatgg aaaaaaatga aaacattaat
     2581 gtagagattt taactacgtc agatatgcat agtcatttct taaatggtga ttatggttca
     2641 aatatttata gagctggtac ttatgttaac caagtaagag cacaaaatca tcgcgtcatt
     2701 ttattagata gtggcggaag tttagctggc tcgttagcgg cctattatta tgctattgtt
     2761 gcaccttata aacgacatcc aatgataaag ttaatgaaca gaatgcatta tgatgctagc
     2821 ggtgtgagtc caagtgaatt caagtttggt ttatcatttt taactcgttc aattgctttg
     2881 gcacgttttc catggttatc agcaaatatt gaatacaatg ttactaagga gccttatttt
     2941 tcaactccat attgtattaa acattttggt gacttaaaaa ttgctatcgt aggcgtcaca
     3001 gcagatggtt taatggaaaa tgagtattct gaaatggagc aagatgtatc tattgaaaag
     3061 acattagtgg catcaaaacg atggattaga tatatccatg aagttgaaga gccagatttt
     3121 ttgattgtaa tttatcatgg tggattgaat aaaattagta atagtacgaa aaataaaaag
     3181 gcaagttcga atgaagctga aaaattaatg gaagaactcg gtgttataga tttaatgatt
     3241 acagctcatc agcatcaaac aatagtaggt caagatcatg aaacgtatta tgttcaggct
     3301 ggtcaagatg ccaaagagct tgtacatctt tcgattaatt ttaaaaagag aacaacaact
     3361 tatgatgttg aaagcattga ttctaaagtg attgatttaa atgagtatga agaggatcaa
     3421 gaattattag atttaacatt ctatgataga aaagcagtgg cttattggtc acaggaaatc
     3481 ataagtgata aaggtttgat gttatcagta aatgggttac aagatttagt ctgtcaaaca
     3541 catccatttt cgcaattatt acatgatgca attcaccttg catttgataa tggtataaca
     3601 tgtgtccacg tgcctatgaa cggagagaag gggctgagtg gacagattcg aaatgaagat
     3661 ttgtatcatg catacccata tccagataag ccaatggata tgacaattag tggtcaaaat
     3721 atcaaagata tattggagta tagttattca catttagatt ttgttaacga gcaattaagc
     3781 ttaacaatta ttgatgaaac attatgtaca atgtggcaag gattcaatta tgagattgat
     3841 atgaatcaag aacctggtca acgagtaatg ttagatcaaa ttgatttgac taagagttat
     3901 agagttacaa tgactgacta ttgttatcgt aactacaaga attatttaaa aaatgctatt
     3961 atacatgaat catacgatga aacaatgagt acattaattg cagagaagtt aagagatccg
     4021 aattatcgta tttcatgtag tgataatttt gtagttaaaa acaggtaaat attgctatgt
     4081 aataaattaa ttataagttt aacaagtata aaaacctttc ttagagaaga ttatctagga
     4141 aaggttttta gaattttaaa attgatataa aagaacagtg aaattctcat tcattatata
     4201 taagtaaaca aaaaagctta aatggtatat accatttaag caagtaacgg aaacggaggg
     4261 attcgaaccc tcgcgccgct ttcgcgacct acacccttag caggggcgcc tcttcagcca
     4321 acttgagtac gtttccaatg gctccacagg taggactcga acctacgacc gatcggttaa
     4381 cagccgatag ctctaccact gagctactgt ggattaattt gtatgtgttt gaatatcaag
     4441 cacaaatatt attataacaa gattaagtta atttgcaata gataccttaa aaaaatatgc
     4501 agaacattta cactatattt ataaaacatt ccgcatattt taaaaataaa caatttattt
     4561 atcttcaaat aaagaatagc gtgtcaatgc ttcaaacaca ttcaatgtgg ctttattata
     4621 atctattggt tggtcgggat tttttgatat catgcgagat tgtttatcta ccatgtaata
     4681 gtaaaaaatg gaattgttat attgatgcca taaactgtgg tatgaaacat gaagaatgat
     4741 agattcatta ttaataaatg tcactttaca tttacgattt tttaattctt taatactttc
     4801 gatataatgc atatttaacc aaatattttc attttgtctg tcagaatgtg ttgggaaaaa
     4861 ataagttgga aataatggtg ttagtaaaat aggtggttta ctagaaatgc cagtaatgcg
     4921 gtttgtctct gctttcttgc caagataagt attaccataa aatttgcaag agcgttcaat
     4981 gattttctgg actttaaaag gattttgaat acgcgttttg tcaaaccgaa ttatttcact
     5041 accgtttctt tgatcgtcat catcaaatgc tggtcgaata accatgtctc ctttgcgtat
     5101 cacataaata ttttgagaat acataagcaa aacctcgctt tattaa
//
LOCUS       DAHHYJ010000160         5121 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000160 DAHHYJ010000000
VERSION     DAHHYJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.160"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..1685)
                     /locus_tag="NBK22_002335"
     CDS             complement(<1..1685)
                     /locus_tag="NBK22_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCD2415502.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNDQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGITLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDL"
     gene            complement(1709..2680)
                     /locus_tag="NBK22_002336"
     CDS             complement(1709..2680)
                     /locus_tag="NBK22_002336"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCD2415503.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLE
                     NFDESIRHRATSIQLHDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(2667..3869)
                     /locus_tag="NBK22_002337"
     CDS             complement(2667..3869)
                     /locus_tag="NBK22_002337"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCD2415504.1"
                     /translation="MDKSLFEQPRPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSSVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFKAMRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKS
                     LKAFNYMPYFEQLDMNQINVTEPIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNLQTIN
                     ETLHRLTMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(3874..5016)
                     /gene="bshA"
                     /locus_tag="NBK22_002338"
     CDS             complement(3874..5016)
                     /gene="bshA"
                     /locus_tag="NBK22_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCD2415505.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
BASE COUNT         1587 a          944 c          677 g         1913 t
ORIGIN      
        1 tgtaaatcct tcaaaatgtc ttttgtttca aaagtaaata cattatggaa gcgatgaata
       61 tcatcatcat aaacatcaga atcattgata atcgtaaata tcgttgagaa caattgctca
      121 tttaactcat gaatctcatt catactagcc ttcaagccaa aaatatcaat tggtgcaata
      181 tctaattttt ccaaaattcg ctgcttttcc agttgatcaa ttgcctttaa caatttttca
      241 ttttcgtttt taccaatcaa accaagctga tatttaatat cagcataact caactcattt
      301 gtcacttgat ttaaggcata gtctggtaag cgatgtgctt catccactat acaatcatca
      361 aacaattgat atattgaatt ttcaacatca gaatgaatta aatgtgcatg atttgtaata
      421 ccaatttgaa tgttctgtgc atttcgctta ataaaattat aataatgaac atcgtgacgt
      481 gccggtacat atgtttcaat tttctggtca aaatacatct tttgaccacc ttttaaattt
      541 aattcctgta tatctccgga cggcgtctct gtaatccaaa ttaacaattg cattttcaag
      601 atattcactt cgtaattact tgtgtcatct tttaaaattt gactaataag tcccaatgaa
      661 atgtaatcac ttttactttt aatcaatagt gcattaattt taaaatttaa cgcttcattc
      721 attgctggaa tatctttttc taacaattga ctttgcagta atttagtatt ggtagaaatc
      781 atgacatgct tcccagtttc aatattatac atcaaggccg caagtaaata tgctaatgat
      841 ttaccactgc ctagtgatgc ttcaatcatt gctttttcac tatgcatgag ctgatctaat
      901 atagtttccg ctaaatataa ttgttgcggt cgatatgtta agccaagttg atctacagct
      961 ttgctatata aagacttcaa gctgccatta taatttgttg tcggcttttt aaaatcaact
     1021 tgcttacgat agataatctg ttcgaacttt tcgtacgatt tatccaatgg ctttgcatca
     1081 tattgcctaa ccatctcaaa gaaaatatca tacaaatcgt atttcaactg tttacttaaa
     1141 taatataatt gcttcaaagt atctaacggt aacttttcaa atttttcaaa agctaatatc
     1201 atcaatttag cagtagtagc ggcatcttcg tcagctcgat gggcatttgc taaggtaata
     1261 ccatgtgcct ctgctaattc acttaattga tagcttttat ctgtaggaaa agctatttta
     1321 aagatttcta gtgtatctat aacttttttg ggacgatatt gaatattaca atctttaaat
     1381 gcctttttaa taaaattcaa atcaaaatct acattatgag cgacaaaaat gcaatctttt
     1441 atcttatcgt agatttcttg tgcaacttga ttaaaatatg gcgcttgttg tagcatattt
     1501 tcttcaatgg atgttaacgc ttgaatgaac ggcggaatct ctaaatttgt tctaatcata
     1561 gaatgatatg tatcaataat ttggtcattg cgcacaaacg ttattccaat ttgaatgata
     1621 tcgtcaaaat ctaattggtt gcctgttgtt tccaaatcca caacggcata ggttgccata
     1681 cccatagcta tctctccttg ctttagtgtt aaaaatctat atctgcacta attaaacggt
     1741 gtgattcacc cgcttcatct ctaacaatta gatagccatc gtaatctaaa tcaattgctt
     1801 gtcctttaaa ctgtttatca ttttctgtaa atagcaacgt tctattccaa atattagaag
     1861 ctgcaatata ttcttcacga atttcggaaa aaggtaacgt taaaaattga ttatatcttt
     1921 tttcaatttc ttgaagtaat ctctctaaaa attgatatct atctaattta tttttatcat
     1981 gtaattgtat acttgttgct ctatgtctaa tactttcatc aaagttttct agttgttgcg
     2041 tcaaattaat acctatacca catattattg cttctatacc atcgttatta gcaaccattt
     2101 cagttaagaa accacacact ttaccattat caatatatat atcattcggc catttcactt
     2161 tgacttcatc ttgactaaaa tgttgaatcg catctcttat ccctaatgca ataaataaat
     2221 taaatttaga tatcattgag aatgcaacgt taggtcttaa cacgacagac atccaaagtc
     2281 cttgcccttt tgaagaactc caatgtctat taaatcgccc acgacctttc gtttgttcat
     2341 cactcaagat aaaaaatgaa gattgatttc caacaagtga ctttttcgca gcaagttgtg
     2401 tagaatctat tgaatcgtat acttcactaa aatcaaacaa agcagaactt tttgtatatt
     2461 ggtctattat accctgatac caaatatctg ggagttgttg taataaatgc cctttatgat
     2521 ttactgaatc aattttacat ccctctaact ttaattggtc aatcaccttt tttactgcag
     2581 tgcgtgaaat attaagtgat tccgcaatgc tttgtccaga tatataattc ggtttatttt
     2641 tatagagtaa ttgaagtaca tcttgactat attttgacat gattatccac ccatttcaaa
     2701 atttcagttt cttcgttgct tactttacct gttacaatcg ctatctcaat ttgtcttagt
     2761 acatctttta accacggacc acttttggca tttaaatgtg ccataagtac accgccatta
     2821 accatcatgt cttttctatt atgcatagtt aaacgatgca atgtttcatt aatcgtttga
     2881 aggttaacga ttaatggttc atgtccttga atatcatttg cctttaacac gtctgctgca
     2941 accattacat ttttaatgag attcgtatca taatcataaa caaacatttt taattgttct
     3001 tttgtaataa tacttggtaa tgcattcata atttgaatat attgattgat atcttttact
     3061 tgtcggttac ttagctttaa aggcttcaat gagtaattaa tatcaaattt aactgacact
     3121 atagcaatca acaattctaa atcaattggt tcagttacat taatttgatt catatcaagt
     3181 tgttcgaaat atggcatata attaaatgct ttcagcgatt ttaaatgatt aaaactctct
     3241 tcaacattaa tacctcgcat taatttagtt agttcaatca ctatacgctc aattgataaa
     3301 aatttaatat ctgccatttg tgtacgcatc gctttgaatg tttccgttgc aatatcaaat
     3361 gataattgtg actggaatct taaacatcga atcatacgta aagcatcttc ttggaatcgt
     3421 tcctcagcta tacctacagt tcttattatt cgattattaa tatcttgttg accatcaaaa
     3481 taatcataca atttgtatgc tgtatccatt gctatcgcat tcatagtgaa atctcttcgt
     3541 tgcaaatctt cgtataaatc acgaacaaat gtaacactac ttggtctacg gtgatcgaca
     3601 taatcttctt cagcccggaa tgttgtcact tcataatttt catcattaaa aactacattt
     3661 atcgtgccat gttctttacc tacaggtatc gtatgactaa agatagattc tatttcatcc
     3721 ggcgttgcac ttgttgtgat atctatatca tgaatatttc ttcccatgac ataatctctt
     3781 acagagccac ctacataata tgcttcaaaa ccattgtctt gaatttgttc taatataggc
     3841 cttggctgtt caaataatga tttatccata ttattactcg cctttacttt tgttatgctc
     3901 atttagcatt ttttgataat aatactcata ttgatctgta atgagttctg atccaaaacg
     3961 ttcagcaata tctgctagca tgtttttctg aagtttgttg tataacacct tatcttcaag
     4021 taatcggata gcatagtcac tcgctgaatc acaatcaccc acatctacga caaatccagt
     4081 ttcaccatgt ttaataacct ctttaattcc accggcattt gaaccaattg gaacgacgcc
     4141 tgttttcata gcctcaagta aagttagtcc aaagctttct ttttcactta ataataatac
     4201 taagtcagat aattggtaaa attcacttac gcaatcttgt ttccctaaaa ataaaacatc
     4261 ctcttctacg tttaactctt tcgtcaattg acgcattggc actaattcag gaccatctcc
     4321 aagtaaaatt aatttactag gtatcttttc acgtactttt gcaaatgttt ctataatagt
     4381 atctatgcgt tttacttgtc taaaattcga tacatgtatt aacacttttt catctggtgc
     4441 tataccaaat tgtgatttta gtgctgtgtt atgtttagtt ggaaactcat tttcacgtac
     4501 aaaattataa atcggtataa tttctttgtt agtttcgata atttcatgtg tttcttgcgc
     4561 taaagattta ctcacacttg tcacaatatc acttttttca atgccaaatt taattgcacc
     4621 ttggagtgaa tgatcatagc ccaaaacagt aatatcagta ccgtgtagcg ttgtcataat
     4681 ttttatatct ttacctgaca tctcacgagc taaaatccca caaattgcat gaggtacagc
     4741 atagtgcata tgcaacaaat caagatcata ttctttaata acttcagcga ttttagtact
     4801 taatgtaata tcatacggtg gatactgaaa tactgcatat tgattcactt caacttgatg
     4861 aaaaatcata ttcggtaatg gttttcttat tctaaacggg atatttgaag tgataaaatg
     4921 tacttcgtga cctcgctctg ctaatttaat tcctaattct gtggcaataa ttccagaacc
     4981 acccatggac gggtaacatg ttatacctat cttcattcgc ttgcccatcc tttctttcta
     5041 tttctctctc tattattctc gatgcgtaga taattgttta aatttaagtt tatagtaatg
     5101 ttgagtttat aatttcaaat a
//
LOCUS       DAHHYJ010000161         5104 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.161, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000161 DAHHYJ010000000
VERSION     DAHHYJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.161"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(17..92)
                     /locus_tag="NBK22_002339"
     tRNA            complement(17..92)
                     /locus_tag="NBK22_002339"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Ala,seq:tgc)
     gene            complement(116..189)
                     /locus_tag="NBK22_002340"
     tRNA            complement(116..189)
                     /locus_tag="NBK22_002340"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Pro,seq:tgg)
     gene            complement(210..286)
                     /locus_tag="NBK22_002341"
     tRNA            complement(210..286)
                     /locus_tag="NBK22_002341"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Arg,seq:acg)
     gene            complement(292..380)
                     /locus_tag="NBK22_002342"
     tRNA            complement(292..380)
                     /locus_tag="NBK22_002342"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(344..346),aa:Leu,seq:taa)
     gene            complement(388..462)
                     /locus_tag="NBK22_002343"
     tRNA            complement(388..462)
                     /locus_tag="NBK22_002343"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(428..430),aa:Gly,seq:gcc)
     gene            complement(496..568)
                     /locus_tag="NBK22_002344"
     tRNA            complement(496..568)
                     /locus_tag="NBK22_002344"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(533..535),aa:Lys,seq:ttt)
     gene            complement(575..650)
                     /locus_tag="NBK22_002345"
     tRNA            complement(575..650)
                     /locus_tag="NBK22_002345"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Thr,seq:tgt)
     gene            complement(667..742)
                     /locus_tag="NBK22_002346"
     tRNA            complement(667..742)
                     /locus_tag="NBK22_002346"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(707..709),aa:Val,seq:tac)
     gene            complement(755..869)
                     /gene="rrf"
                     /locus_tag="NBK22_002347"
     rRNA            complement(755..869)
                     /gene="rrf"
                     /locus_tag="NBK22_002347"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1416..2903)
                     /gene="lysS"
                     /locus_tag="NBK22_002348"
     CDS             complement(1416..2903)
                     /gene="lysS"
                     /locus_tag="NBK22_002348"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCD2415506.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            complement(2956..3048)
                     /locus_tag="NBK22_002349"
     CDS             complement(2956..3048)
                     /locus_tag="NBK22_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415507.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            complement(3442..3918)
                     /gene="folK"
                     /locus_tag="NBK22_002350"
     CDS             complement(3442..3918)
                     /gene="folK"
                     /locus_tag="NBK22_002350"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCD2415508.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRIRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVVEPRSKLKVKDLVFVDDSVKRYK"
     gene            complement(3915..4280)
                     /gene="folB"
                     /locus_tag="NBK22_002351"
     CDS             complement(3915..4280)
                     /gene="folB"
                     /locus_tag="NBK22_002351"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCD2415509.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            complement(4258..5061)
                     /gene="folP"
                     /locus_tag="NBK22_002352"
     CDS             complement(4258..5061)
                     /gene="folP"
                     /locus_tag="NBK22_002352"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCD2415510.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAINRVKAMIDEGADII
                     DVGGVSTRPGHEMVSLEEEMNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDMI
                     NDQWAGLYDHRMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
BASE COUNT         1569 a         1115 c          707 g         1713 t
ORIGIN      
        1 tcgtgtaaaa aataaatggt ggagactagc gggatcgaac cgctgacctc ctgcgtgcaa
       61 agcaggcgct ctcccagctg agctaagccc ccataaaatg gaatttaaga agtaatcggg
      121 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
      181 tacttcccgt tattcaatta atttaaaaat ggcgcgcccg ataggagtcg aacccataac
      241 ctcttgatcc gtagtcaaac gctctatcca attgagctac gggcgctaaa atggtgccga
      301 ggaccggaat cgaaccggta cgatgatctc tcaccgcagg attttaagtc ctgtgcgtct
      361 gccagttccg ccaccccggc aaaataatgg agcagaagac gggattcgaa cccgcgaccc
      421 caaccttggc aaggttgtat tctaccgctg aactacttct gcattatttt gctaaatata
      481 aaaattaaat cgtaatgagc catagaggat tcgaacctct gaccctctga ttaaaagtca
      541 gatgctctac cagctgagct aatggctcaa aagatggtgc cggccagagg acttgaaccc
      601 ccaacctact gattacaagt cagttgctct accaattgag ctaggccggc tatatctaag
      661 aataaatggt ggagaatgac gggttcgaac cgccgaccct ctgcttgtaa ggcagatgct
      721 ctcccagctg agctaattct ccgatttgtc atttgcctgg caacgttcta ctctagcgga
      781 acgtaagttc gactaccatc gacgctaagg agcttaactt ctgtgttcgg catgggaaca
      841 ggtgtgacct ccttgctata gccaccagac aaaataactt acttatttat gatacacgct
      901 taattaataa ggtgcaatag ttaattttac actttgttgt gaactttgtc acatcagctt
      961 ttagtgtgtt tcgtctgttt ttgacgactt ttatatatta cttcattcaa ttgataaagt
     1021 caacactttt aatcaacttt tttattcttc aaaactgaca tttctcgtgc tctctaattc
     1081 ataacacttt tagtattcta tcatacacat atgtacaaaa ccatacacat tctatacaat
     1141 tttaaagtac ttttatagct gattaagata aattatacta atatagcaat ttttgttata
     1201 agatatctca atgtctatcg ttaacatttc tcatccgaac tatatcgtca ttttcatcta
     1261 tcagccaacc accttgttta acagtcaaaa atagcaccac tatcgttaac tattactatc
     1321 aaaaacattc aaacacgaac taattaccat aattaagaaa accctcgcat acacatgttg
     1381 tatacacgag agctcttact aacaatcaac gtcatttatt tttgtctcat ataagggaat
     1441 aataacacgt ctctgattga tggagaatca gttaataaca taaccaatct gtcaataccg
     1501 ataccaagac cacctgtcgg aggcatacca tattctaacg cttcaatgta atcttcatcc
     1561 atttcatgcg cttcatcatt accttgcgct ttttcaacaa gttgcgcttc aaaacgacct
     1621 ttttgatcaa taggatcatt taattcagta aatgcatttg catgctctct acctacaatg
     1681 aacaattcga aacgatcagt aaatctagga tcttcaggat ttttcttcgc taaaggtgaa
     1741 atttcagtcg gatgaccata gataaacgtt ggctgaataa gtgtttcttc aactttttgc
     1801 tcaaagaatt catttaaaat atgaccatat ttcattgtat ctttaatttc aataccatgt
     1861 tctttagcta aagctttagc ttcttcatca cttttaactt cataaaaatc tacaccagta
     1921 gcttctttta cagcatcaac aatatgcaaa cgagtccaag cagattctaa atcaatcgtt
     1981 tccccattgt attgtacttt tgcagaacct aatacttcat tggcaatatg tctcaccata
     2041 gattctgtta aatccataat gtcatgataa tctgcatatg cttcatataa ttcaatcatt
     2101 gtgaattcag ggttatgtct agttgataca ccttcattac ggaatactct accaatttca
     2161 tatacttttt caagtccacc gacaattaaa cgttttaaat gcaactcaat agcaatacgc
     2221 atgtataacg ttgcatctaa tgcattatga tgtgttacaa atggtctagc agctgcgcca
     2281 ccagcaattt ggtgcatcat aggtgtttct acttccaaga aacctttatt atttaaataa
     2341 ttacgcattt cttgaatgat tttactacga ttaataaatg tacgagtgct atcttcgttc
     2401 gtaattaaat ctaaatatct ttgacgatat ctctgttcaa tatcctgtaa accgtggaat
     2461 ttatccggta atggtcgcaa tgatttagtt agtagcgtga atttcttcgc tttaaccgat
     2521 aattcgccag tatttgtttt gaacattaca ccttcaacac caacgatatc gcctaaatca
     2581 gcatttttcc ataaatcaaa ttcgtcatcg ccaacttgat ctttacgaac gtaaatttga
     2641 atttgtccag ctaagtcctg aacgtgtgca aatcctgctt tacctttacc acgcttagtc
     2701 attaatcgtc cagctatagc gacatgacta tccgcttctt tttctaccaa ttcttcttta
     2761 gaatactggt cccactcttc tttcaaatca ctagataaac ctgaacggtc aaatttagaa
     2821 ccaaacgggt ctataccaag atcatataat tcttgtaatt tttgacgtcg aaccaacatt
     2881 tggtcattca tttcttctga cataactttc tctcctttaa cttgttgtaa tcctttagta
     2941 ttccaactgg tacactcaaa cattttcctt taaagtttta taaaatatca gacatttttg
     3001 ctcaacatac atctctttcg atgtttctaa tactttatca ttaaccatta caccttaaag
     3061 ttatgatgtg gcatgttttc ttatattcat aacatcaatt ttatcacttt tttaatttta
     3121 caagagcttg taaatgtaat aaagcaattt ctgttttcgt ttatgaatta cttattcatt
     3181 taaaatacga ttatcattaa tacaacccta agtaaatgta taaaaattat cttccacaaa
     3241 cttcaacaaa agcccaaata aattacagca atttatcaaa tattgcttac tttgatttta
     3301 tgaaataact taattctaac acatactaaa tcatcatata ctaattcgaa atcaaatgca
     3361 tttagagata atcaaaatgc ggaaacatct ccaataatca atcatctatt cccaataaat
     3421 atgaatgttc tcaacaatgc attatttata tctctttaca ctgtcatcga caaaaactaa
     3481 atctttcact ttcaatttcg aacgtggttc tacgacattt gctgctatat catttaatgg
     3541 gattaaaaca aatgcacgtt cattcattct cggatgtggc accgacagtt ttggtaaatc
     3601 tatcatttct tctccataca acaaaatatc cacatctaaa gttctaggac cccatcgttc
     3661 ctttctaata cggtgtaaac attcttctgt cttcaaacaa cattccaaca gttgtaatac
     3721 tgtgagtgtt gtttgaattt caacacacaa atttaaaaag ttaggttgct cagtataccc
     3781 aactggtgct gtttcataaa tcggagaaat attagatacg ttaataccat catattcatt
     3841 caaaatcttt atagcatcgt ttaactggct ttctctatca ccaatattac tacctaatcc
     3901 taagtatgct tgaatcattt attctccctc actatttcga tacctactcc atcataatga
     3961 cccggaatcg gtgggttttc tttagtgatt ctcactttcg tttccattac acgattatat
     4021 tgtgaattta tacgatttgc aatacgttca gctagatgct caagtaaatt aacggcctta
     4081 ccttccataa ttgatttaac ctcttcgaac acttcaccat aatgaactgt atcaataaca
     4141 ttatcagtac gcccagcttc agctaagtct actttcaaag ttacatccac tttgaaaatt
     4201 tgtcctattt cattttcagc tgataaagca ccatgatatc cataaaagcg cataccttta
     4261 agaaagattg tgtcttgcat tttcattctc ctttaaaaaa tctatacctt tagctaattt
     4321 agcattcaac tcgacattat gaacgcgtac tgctctaacg cctttcataa taccatatgc
     4381 agtcgtagct gcagttactt catctctttc aaccggtgtt gtatcataac ccatcatctc
     4441 tttagtgaaa cgtttccggc ttgtcgctaa taaaactgga tattctgttg caacaagttc
     4501 atccagtctt gccataactt cggcttcttc atttctagtt ttagcgaaac ctatacctgg
     4561 atctagccaa attttatttg aaggtatacc agctatttta gcttgatgtg cttgtgctaa
     4621 caaagatgtt aacatttctt cgacaaccgg ttcatcacga ttaccatttc cattatgcat
     4681 taaaataatt tccgcgtcat atttagctac aatttggaac atacgatgat catacagacc
     4741 cgcccattga tcattaatca tatcaacacc taattttaaa catgcttcag caacctcact
     4801 tcgaaatgta tcgactgaaa tttttacatc aaaaccgaca atagcttcaa caacaggtaa
     4861 tactctgttc atctcttctt ctaatgaaac catttcatga cctggtctcg ttgaaacacc
     4921 tccaacatct ataatgtcag caccttcatc tatcatggct ttcactctat ttatagctga
     4981 ttcaacatta ttaaattttc caccatctga gaatgaatca ggtgtgacgt ttaatatgcc
     5041 cataattttt gttttagtca tgttaaccac cctttaatta tgtattgttt aactctaata
     5101 aaat
//
LOCUS       DAHHYJ010000162         5017 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000162 DAHHYJ010000000
VERSION     DAHHYJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.162"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            18..863
                     /locus_tag="NBK22_002353"
     CDS             18..863
                     /locus_tag="NBK22_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCD2415511.1"
                     /translation="MSELSKSEDQRITKTKDEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGAKVDISIKKSIGKISFEFESQDDFLRIIEQLN
                     RRYGK"
     gene            1020..1901
                     /locus_tag="NBK22_002354"
     CDS             1020..1901
                     /locus_tag="NBK22_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCD2415512.1"
                     /translation="MNQVMNIISSLFEPLTKIETYENIATKIAMIIIYIIVALIVIKI
                     LNKMIEQGFKIQNKSKKSNKKRSKTLISLVQNVVKYIVWFIVITTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFESQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVEENIDNVDKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAIEDTRVILRISAETIPGEGFAGARIIRKEIQKMFLQEGI
                     KTPQPIMTAYNHSENGV"
     gene            1931..2134
                     /locus_tag="NBK22_002355"
     CDS             1931..2134
                     /locus_tag="NBK22_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCD2415513.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIESHDDTQR"
     gene            2146..3243
                     /gene="ychF"
                     /locus_tag="NBK22_002356"
     CDS             2146..3243
                     /gene="ychF"
                     /locus_tag="NBK22_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCD2415514.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILTTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(3329..3520)
                     /locus_tag="NBK22_002357"
     CDS             complement(3329..3520)
                     /locus_tag="NBK22_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415515.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            3764..4060
                     /locus_tag="NBK22_002358"
     CDS             3764..4060
                     /locus_tag="NBK22_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCD2415516.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            4081..4584
                     /gene="ssb"
                     /locus_tag="NBK22_002359"
     CDS             4081..4584
                     /gene="ssb"
                     /locus_tag="NBK22_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD2415517.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            4636..4878
                     /gene="rpsR"
                     /locus_tag="NBK22_002360"
     CDS             4636..4878
                     /gene="rpsR"
                     /locus_tag="NBK22_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCD2415518.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
BASE COUNT         1861 a          681 c          998 g         1477 t
ORIGIN      
        1 atcaaataaa aagtgatgtg agtgaattgt caaaaagtga agatcaacgt attactaaaa
       61 caaaagatga acaaattaag caaatagata tatcggatat caaaccgaat ccgtatcagc
      121 cccgaaaaac tttcgatgaa aatcatttaa atgatttggc agattcaatt aagcaatatg
      181 gtattttgca accaattgtg cttagaaaaa cagttcaagg ttattacatt gtagttggtg
      241 aaagaaggtt tagagcttcg aaaattgctg gtctaaaata cgtatcagcg attatcaaag
      301 atttaacaga tgaagatatg atggaactgg cggtcatcga aaatttacaa cgagaagact
      361 taaatgcgat tgaagaagct gaaagttatc aacgtttgat gacagatttg aaaattacac
      421 aacaagaagt agcgaagcga ttgagtaagt cgcgcccgta tatagcgaat atgttgaggt
      481 tattacattt gccgaaaaag attgctgaca tggtaaaaga tgggcgactg acaagtgcac
      541 atggacgaac gttattggca attaaagatg aacaacaaat gcttaggtta gcgaaacggg
      601 ttgttaaaga aaagtggagt gtcagatatt tagaaaacca tgttaatgaa ttaaaaaatg
      661 tttcgtcaaa gtcggaaaca gacaaagtag atataactaa gcctaaattt ataaagcaac
      721 aagaacgaca gttgcgagaa cagtatggcg caaaagtaga tatatcgata aaaaaatcaa
      781 tcggcaaaat ctcatttgag tttgaatcac aagatgattt tctaagaata attgaacaat
      841 taaatcgtag gtatggtaaa tagttacaca attttatata ataactcttt gtgcaagtgt
      901 aaataaattg taatcagtga acatttgatt ctagatatat tgagactttc gtaggttgga
      961 agtatataaa aatatgaata caatattgat atatcatata gaagggagca acgcttatca
     1021 tgaatcaagt catgaatatt atttcatctc tatttgagcc attaacaaaa atagaaacat
     1081 atgaaaacat tgcaactaaa atcgctatga ttattattta tattatcgta gctctcatag
     1141 ttattaaaat actgaataaa atgattgaac agggatttaa gattcaaaat aaaagtaaaa
     1201 agagtaacaa aaagcgctct aaaactttaa tatctcttgt tcaaaatgta gtgaagtata
     1261 tcgtttggtt tatagttatt acgacgattt taagtaaatt tggcattagt gttgaaggtg
     1321 ttattgccag tgccggtgtc gtaggtttag cagtaggttt tggtgctcaa accattgtta
     1381 aagacgttat tactggattt ttcattatat ttgaaagtca atttgatgta ggtgattatg
     1441 ttaaaataaa caatggtggt acgactgtgg cagagggaac ggttaaatca ataggacttc
     1501 gttcaacacg aatcaataca atttcaggag aattaacaat tttaccaaat agtagtatgg
     1561 gtgaaataac gaactactca attacaaatg gtacagctat cgttaaaatt ccagtgtctg
     1621 tcgaagaaaa cattgataat gttgataaaa aactaaacaa actatttact tctttacgta
     1681 gtaaatatta cttatttgtt agtgatccgg ttgttattgg tattgatgct attgaagata
     1741 caagagtaat attgagaata tctgcagaaa caattccagg tgaaggattt gctggagctc
     1801 gaattattcg caaagaaata caaaaaatgt ttttacaaga aggtattaaa acacctcaac
     1861 caattatgac tgcttataat catagtgaaa acggtgttta gtagtttata atacatggag
     1921 gtcatattta atggcgtcaa aatatggaat aaatgatata gtagaaatga aaaaacaaca
     1981 tgcgtgtgga acaaaccgtt ttaagattat tagaatgggt gcagacataa gaattaaatg
     2041 tgaaaattgt caaagaagta ttatgattcc acgtcaaacg tttgataaaa aacttaaaaa
     2101 aatcatcgaa tctcatgatg atacacaaag ataggagaat gattaatggc tttaacagca
     2161 ggtatcgttg gattgccaaa cgttggtaaa tcaacattat ttaatgcaat aacaaaagca
     2221 ggtgctttag cagcgaacta tccattcgct acgattgatc ctaatgtagg gatagtagaa
     2281 gtgccagatg ctagattact taaattagaa gaaatggttc aacctaaaaa gacattgccg
     2341 actacatttg aatttacaga tatcgctggt attgtgaaag gtgcttcaaa gggagaaggg
     2401 ttaggtaata aatttttatc acatattaga gaagtagatg cgatttgtca ggtcgttcgt
     2461 gcatttgatg atgataacgt aactcatgtt gctggtcgag tagaccctat tgatgatatt
     2521 gaagttatta atatggaatt agtactagcg gacttagaat ctgttgagaa acgtttgcct
     2581 agaattgaaa aattagcacg tcaaaaagac aagactgctg aaatggaagt acgtatttta
     2641 acaactatta aagaagcttt agaaaatggt aaacccgctc gtagtattga ctttaatgaa
     2701 gaagatcaaa aatgggtgaa tcaagcgcaa ttactgactt ctaaaaaaat gctttatatc
     2761 gctaatgttg gtgaagatga aattggtgat gatgataatg ataaagtaaa agcgattcgt
     2821 gaatatgcag cgcaagaaga ctctgaagtg attgttatta gtgcaaaaat tgaagaagaa
     2881 attgctacat tagatgatga agataaagaa atgttcttag aagatttagg tatcgaagaa
     2941 ccaggattag atcgattaat tagaacaact tatgaattat taggattatc aacatatttt
     3001 actgctggtg tgcaagaagt acgtgcttgg acatttaaac aaggtatgac tgcacctcaa
     3061 tgtgctggta tcattcatac tgattttgaa cgtggattta tccgtgccga agtaacaagt
     3121 tatgacgact atgtacaata tggtggcgaa agtggcgcta aagaagcggg cagacaacga
     3181 ttagaaggta aagaatatat tatgcaagat ggcgatatcg ttcatttcag atttaatgta
     3241 taaacgatag agtgaagtta attaaatagt atatatggag aagaggcgga atcaattgtt
     3301 cgcctctttt aattatgcgt ataatttatt aaaagaatgg aataatttta ctcgcgttaa
     3361 taatatcttg agtgctgaaa aattgtttgc cttcgccagt ataagcaggc tctaaaacaa
     3421 gattagcctt tgcacaataa agccattcag gatgaatgcc actattaagt atctcttgga
     3481 attcttgaaa atctttagac caatcaatat ttaaattcat tccttaccac ctcacaacta
     3541 ttatagaaca tgcgttcgct tttgtgaagt gtatttttaa aaatatcata gaaaatttca
     3601 aatgaattaa tgtcaaaaat gatatgaaga gtagtttgaa ttattattgt aaaaagtaat
     3661 ggcgcatgat ataattcttt attgtgagta atgaaaatta ttccttgctt atctgtttta
     3721 agattgataa gccgtataga ccacaaggag gtgcaaatat aaaatgagaa catatgaagt
     3781 tatgtacatc gtacgcccaa acattgagga agatgctaaa aaagcgttag ttgaacgttt
     3841 caacggtatc ttagctactg aaggtgcaga agttttagaa gcaaaagact ggggtaaacg
     3901 tcgcctagct tatgaaatca atgatttcaa agatggcttc tacaacatcg tacgtgttaa
     3961 atctgataac aacaaagcta ctgacgaatt ccaacgtcta gctaaaatca gtgacgatat
     4021 cattcgttac atggttattc gtgaagacga agacaagtaa taattagagg gggcgtttaa
     4081 atgctaaata gagttgtatt agtaggtcgt ttaacgaaag atccggaata cagaaccact
     4141 ccctcaggtg tgagtgtagc gacattcact cttgcagtaa atcgtacgtt cacgaatgct
     4201 caaggggagc gcgaagcaga ttttattaac tgtgttgttt ttagaagaca agcagataat
     4261 gtaaataact atttatctaa aggtagttta gctggtgtag atggtcgctt acaatcccgt
     4321 aattatgaaa atcaagaagg tcgtcgtgtg tttgttactg aagttgtgtg tgatagcgtt
     4381 caattccttg aacctaaaaa tgcgcaacaa aatggtggcc aacgtcaaca aaatgaattc
     4441 caagattacg gtcaaggatt cggtggtcaa caatcaggac aaaacaattc gtacaataat
     4501 tcatcaaaca cgaaacaatc tgataatcca tttgcaaatg caaacggacc gattgatata
     4561 agtgatgatg acttaccatt ctaataaaaa ttaacgaaat taaagcgaaa aaattatcaa
     4621 aggaggcaca caatcatggc aggtggacca agaagaggcg gacgtcgtcg taaaaaagta
     4681 tgctatttca cagcaaatgg tattacacat atcgactaca aagacactga attattaaaa
     4741 cgttttatct cagaacgcgg taaaatttta ccacgtcgtg taactggtac ttcagctaaa
     4801 tatcaacgta tgttgactac agctatcaaa cgttctcgtc atatggcatt attaccatat
     4861 gttaaagaag aacaataata tataatttat tgtcaaaccc cgtaggcata ggcttacggg
     4921 gctttttgtg ttttggggta tagaaaaagg gcaaaaaagg atgatgtgaa tgttttgtgt
     4981 tcggaatttg cacaaagata tgtttatatt gcaaaaa
//
LOCUS       DAHHYJ010000163         5003 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000163 DAHHYJ010000000
VERSION     DAHHYJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.163"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            53..379
                     /locus_tag="NBK22_002361"
     CDS             53..379
                     /locus_tag="NBK22_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCD2415519.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            381..860
                     /gene="rsbW"
                     /locus_tag="NBK22_002362"
     CDS             381..860
                     /gene="rsbW"
                     /locus_tag="NBK22_002362"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCD2415520.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKNENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            835..1605
                     /gene="sigB"
                     /locus_tag="NBK22_002363"
     CDS             835..1605
                     /gene="sigB"
                     /locus_tag="NBK22_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCD2415521.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHQ"
     gene            2038..4188
                     /locus_tag="NBK22_002364"
     CDS             2038..4188
                     /locus_tag="NBK22_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCD2415522.1"
                     /translation="MDNQLINSIIEKYQFSKKQIEAVLTLLEEKNTVPFIARYRKEQT
                     GGLDEVQIKQIDDEYQYMVNLQKRKEEVIKNIEQQGLLTEELKNDILKQNKLQRVEDL
                     YRPFKQKKKTRATEAKRKGLEPLAIWMKARKHEVSIEEKAQQFINEEVQSVEDAIKGA
                     QDIIAEQISDNPKYRTKILKDMYHQGVLTTSKKKNAEDEKGIFEMYYAYSEPIKRIAN
                     HRVLAVNRGEKEKVLSVKFEFDTTSVEDFIARQEINHNNVNRSYILEAIKDSLKRLIV
                     PSIEREIHADLTEKAENHAIDVFSENLRNLLLQPPMKGKQILGVDPAFRTGCKLAVIN
                     PFGTFIAKGVIYPHPPVSKKEAAEKDFVQMVKAYDVQLIAIGNGTASRETEQFVADLI
                     KKHQLPVQFIIVNEAGASVYSASEIARDEFPDFQVEERSAVSIGRRVQDPLSELVKID
                     PKSIGVGQYQHDVNQKALENALTFVVETAVNQVGVDVNTASSSLLQYVSGLSSQIAKN
                     IIAYREENGAIKHNKELSKIKRLGAKTFEQSIGFLRIVDGSEPLDNTSIHPESYKVTY
                     QLLDKLGFGGNDLGSDALKDKLNSLDMDELAIELQVGVPTLEDIIKSLKAPNRDPRDE
                     FETPILKSDVLSIEDLQEGMKLSGTVRNVVDFGAFVDIGVKQDGLVHVSKLSKKFVKN
                     PMDIVSVGDIVDVWIYSIDKNKDKVSLTMIDPHE"
     gene            4181..4636
                     /locus_tag="NBK22_002365"
     CDS             4181..4636
                     /locus_tag="NBK22_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCD2415523.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFRHCAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDQDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTNRMRCGHCNGKLRMKRQLK"
BASE COUNT         1910 a          627 c          968 g         1498 t
ORIGIN      
        1 tacagcgctt aaaatgaaga taaatatttt taataagtag gagtgtaatg aaatgaatct
       61 taatatagaa acaaccactc aagataaatt ttacgaagtt aaagtcggtg gagaattaga
      121 tgtttatact gtgcctgaat tagaagaggt tttaacacct atgagacaag atggaactcg
      181 tgatatttat gttaatttag aaaatgtgag ttatatggat tcgacaggtt taggtttatt
      241 cgtaggtaca ttaaaagcat taaaccaaaa tgataaagaa ctatacattt taggtgtgtc
      301 agatcgtatc ggtagactat ttgaaattac tggtcttaag gatttaatgc atgttaatga
      361 aggaacggag gtcgaataac atgcaatcta aagaagattt tatcgaaatg cgcgtgccag
      421 catcggcaga gtatgtaagt ttaattcgtt taacactttc tggcgttttt tcgagagctg
      481 gtgctacata tgatgatatt gaagatgcca agattgcagt tagtgaagct gtgacaaatg
      541 cagttaaaca tgcatacaaa gaaaataaca atgtaggtat tattaacata tattttgaaa
      601 ttttagaaga taaaattaaa attgttattt ctgataaagg tgacagtttt gattatgaaa
      661 caactaaatc aaaaataggt ccttacgata aaaacgaaaa tatagacttt ttacgcgaag
      721 gtggcctagg tttattttta atcgaatctt taatggatga agtgacagta tataaagaat
      781 ctggtgtgac aatcagtatg actaagtata taaaaaaaga gcaggtgcga aataatggcg
      841 aaagagtcga aatcagctaa tgaagtttca cctgagcaaa ttaaccaatg gattaaagaa
      901 caccaagaaa ataagaatac agatgcacag gataagttag ttaaacatta ccaaaaacta
      961 attgagtcat tggcatataa atattctaaa ggacaatcac atcacgaaga tttagttcaa
     1021 gttggtatgg ttggtttaat aggtgccata aatagattcg atatgtcctt tgaacggaag
     1081 tttgaagcct ttttagtacc tactgtaatc ggtgaaatca aaagatatct acgagataaa
     1141 acttggagtg tacatgttcc gagacgtatt aaagaaattg ggccaagaat caaaaaagtg
     1201 agcgatgaac taaccgctga attagagcgt tcaccttcta tcagtgaaat agctgatcga
     1261 ttagaagtct cagaagaaga agtgttagaa gcaatggaaa tgggacaaag ttataatgcg
     1321 ttaagtgttg atcattccat tgaagctgat aaagatggtt caactgttac gctattagat
     1381 attatggggc aacaagatga ccattatgac ttaactgaaa aacgtatgat tttagaaaaa
     1441 atattaccta tattatctga tcgcgaacga gaaatcatac aatgtacgtt tattgaagga
     1501 ttgagtcaaa aagagacagg tgagcgtatc ggtttaagtc aaatgcatgt atcacgactt
     1561 cagagaacgg caattaagaa attacaagaa gcagcacatc aatagaattt gtttattaat
     1621 gatacgtttt ataatgaaaa atccatataa ttatcccttg attattaaat tgaaatcgag
     1681 gggtattttt aatttaatta agattttcga attaatacat tattagtgta gtttaatgtg
     1741 tatccacata aatgtcgcga tatagtatta ataatttaag tgaagaagat atctaattgt
     1801 cgttttaaat aggtgggttg ctattagaat aaaaaagtag tcttagatta tgaaatttag
     1861 aaatgatggt gtgtcatttt caataatctt agtgcgtttt aaaatatagt atgacctaat
     1921 cattcgtttt aaatgttttg gaagtgaaaa ttacattaag tatcatacct taatagaagt
     1981 attttagaat atgttaaaat aaatgagtaa atttaagaaa aagtgtgggt taagtaaatg
     2041 gacaatcaat tgattaattc aatcatagag aaatatcaat ttagtaaaaa acaaattgaa
     2101 gcagtattaa cactgctaga agaaaaaaat acagtaccat ttattgcgag gtatcgaaaa
     2161 gagcaaactg gtggactaga tgaagttcaa ataaagcaaa ttgatgacga ataccaatat
     2221 atggtcaatt tacaaaaacg taaagaagaa gttatcaaaa atatagaaca gcaaggatta
     2281 cttactgagg aattaaagaa cgatatttta aaacagaaca aattacaacg tgttgaagac
     2341 ctatataggc cttttaaaca aaagaaaaag acaagggcaa ctgaggcgaa acgtaaaggg
     2401 ttagagccat tagcgatatg gatgaaggca cgtaaacatg aagtctcaat tgaagaaaaa
     2461 gcacaacaat ttataaatga agaagtgcaa tcggttgaag atgctatcaa aggtgcacaa
     2521 gatattattg cggaacaaat ttcagataat cctaaatata gaacaaaaat tttaaaagat
     2581 atgtatcatc aaggtgtgtt aactacatct aaaaagaaaa atgctgaaga tgaaaaaggt
     2641 atttttgaaa tgtactatgc atatagtgag ccaattaaac gcattgctaa tcatagagtt
     2701 ttagctgtta atcgtggtga aaaagagaaa gtattatctg taaagtttga attcgatacg
     2761 acatcagtag aggatttcat tgcacgtcaa gaaatcaatc ataataatgt aaatcgcagt
     2821 tatattttag aggcgattaa agatagcttg aaacgcttaa ttgtcccttc gatagagcgt
     2881 gaaatccatg ctgatttaac tgaaaaagct gaaaatcatg caatagatgt ttttagtgaa
     2941 aacttaagaa atctattact gcaacctcca atgaaaggta aacaaatatt aggcgtagat
     3001 ccagcattta gaacaggttg taaattagca gtcattaacc cattcggtac ttttatagca
     3061 aaaggtgtga tttatccgca tccaccagtt tctaaaaaag aggcagcaga gaaggatttt
     3121 gtacaaatgg ttaaagcgta tgatgtgcaa ttaattgcaa ttggcaatgg tactgcaagt
     3181 cgtgaaacag aacaatttgt tgcagattta attaaaaagc atcagttgcc agtacaattc
     3241 atcattgtca atgaagcggg cgcttcagta tactcagcat cagaaattgc tagagatgaa
     3301 tttcctgatt ttcaagtgga agaaagaagt gccgtatcaa ttggacgacg cgttcaagat
     3361 ccattaagtg aattagttaa aatcgatcct aaatctattg gtgtcggaca ataccagcat
     3421 gatgttaatc aaaaagcact agaaaatgca ttaactttcg tcgttgaaac agcagttaac
     3481 caagtaggtg ttgatgtcaa tacagcatca tcatcattat tacaatatgt gtctggtcta
     3541 agttcgcaaa ttgctaaaaa tataattgca tatagagaag aaaatggcgc aataaagcat
     3601 aataaagagt taagcaagat taagcgtttg ggtgctaaaa cttttgagca gagtatagga
     3661 tttttaagaa tagtagacgg ttcagagcca ttggataata catcgattca tcccgaaagc
     3721 tataaagtaa cgtatcagtt attagataaa ctcggatttg gtggcaatga tttaggtagt
     3781 gatgcattaa aagataaatt aaattcattg gatatggatg aactggcgat tgaattacaa
     3841 gtcggtgtac ctacattaga ggatattatt aaatccttga aagcacctaa tcgagatcca
     3901 agggacgagt ttgaaacacc aatccttaaa tccgatgtgc tatcaattga agatttacaa
     3961 gaaggaatga aattgagtgg cacagttaga aatgtagtag attttggagc atttgtagat
     4021 ataggtgtta aacaagatgg acttgtccat gtttcgaaac tttctaagaa gttcgtaaaa
     4081 aatccaatgg atattgtcag tgtcggtgat atcgttgacg tttggattta cagtattgac
     4141 aaaaataaag acaaagtatc attaacgatg attgatccac atgaataatg acaaacttca
     4201 gcgaatggtt gagaaccttt cggaagaaaa atttggacgt acgtttcgac attgtgcata
     4261 ttttaataaa aggttacgta caacaggcgg acgttatctt ttaaaatccc atgatattga
     4321 aattaatcca aaacaatatg agcattatgg agaagatgct gttgtaaaaa ttatactgca
     4381 cgaattatgt cattatcact tgcatattgc tggtaaaggt tatcaacata aagatcaaga
     4441 ttttaaacga ttgagccaac aggttggagc gcctagattt tgtaacagca ttgaaagcta
     4501 tcaacaacgg gcaaattatg aatattattg cactaaatgt catgcaaaat atatacgtat
     4561 ccgtaaagtt gatacgaatc gtatgcgttg cggacattgc aatggaaaat taagaatgaa
     4621 aagacaattg aaataagtac aatacaaata gagctaattc gtgtatatgt aagattattt
     4681 atgagttgta tggaatttct atagaattta tcagtgagtt attttgattg gtaaacatag
     4741 taattgaata agaagaaatt gtgtttataa aatgtttaaa gccgagattg tcgtaattta
     4801 atacttcgca atctcggctt ttctacttat gatataggta atggcataaa ttgtgacttg
     4861 ataggatgaa attaaattga ttagattaaa aatcttgacg tattttagta ataaataata
     4921 tataaacttt acccaattaa agctttaaag tagcacaaaa gataattata catttttaat
     4981 gacattttga ttgaactttt cta
//
LOCUS       DAHHYJ010000164         4996 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000164 DAHHYJ010000000
VERSION     DAHHYJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.164"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..235)
                     /locus_tag="NBK22_002366"
     CDS             complement(<1..235)
                     /locus_tag="NBK22_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCD2415524.1"
                     /translation="MEKQLQIRKQSAFTMIEMLVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISFITELNYIKSQAIANQGYINVRFYEN"
     gene            complement(213..524)
                     /locus_tag="NBK22_002367"
     CDS             complement(213..524)
                     /locus_tag="NBK22_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCD2415525.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            complement(538..1608)
                     /locus_tag="NBK22_002368"
     CDS             complement(538..1608)
                     /locus_tag="NBK22_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCD2415526.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQ
                     LFKTYFVTNELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQIL
                     EKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILG
                     LFIVAIYLVIMLPMFQMMQSIK"
     gene            complement(1580..2554)
                     /locus_tag="NBK22_002369"
     CDS             complement(1580..2554)
                     /locus_tag="NBK22_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCD2415527.1"
                     /translation="MKILFQEIINKAIEMNASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            complement(2606..3229)
                     /locus_tag="NBK22_002370"
     CDS             complement(2606..3229)
                     /locus_tag="NBK22_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD2415528.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSIQDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(3226..3555)
                     /locus_tag="NBK22_002371"
     CDS             complement(3226..3555)
                     /locus_tag="NBK22_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCD2415529.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(3555..4541)
                     /locus_tag="NBK22_002372"
     CDS             complement(3555..4541)
                     /locus_tag="NBK22_002372"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCD2415530.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFTPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(4538..4741)
                     /locus_tag="NBK22_002373"
     CDS             complement(4538..4741)
                     /locus_tag="NBK22_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCD2415531.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(4722..>4996)
                     /locus_tag="NBK22_002374"
     CDS             complement(4722..>4996)
                     /locus_tag="NBK22_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415532.1"
                     /translation="YADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFEL
                     AIANRSLNDDEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK"
BASE COUNT         1627 a          880 c          654 g         1835 t
ORIGIN      
        1 tgttttcata aaatctaaca ttgatatatc cttgatttgc tatagcttgc gacttaatat
       61 aattcaattc agtaataaaa gaaatgatat ttgcctcatc atctattact ctaagattgc
      121 ttaatccttt agatgtcatt gtcaaaagta gaaatatact gattaacatc attaccacaa
      181 gcatctcaat catagtaaat gctgactgct ttctaatttg caactgcttc tccattacta
      241 attgttattg tctctcctga tttacatgtc ttttgtgctt cttttataaa accatctgca
      301 attaagtctt caatagacga tggatttcta ttatgtttca atgcatacgc ttcaatttga
      361 ctattaacca tttttacctg tgcattacaa cctgttgatt gtatgtgagc agtttgttta
      421 gcaatatttg gaatgattaa aattaataat aaactgatga ttaataacac taatagcatc
      481 tctatcaatg taaacgcttg agttttctta agaaatttaa acatatatta acctccatta
      541 ttttatactt tgcatcattt gaaacattgg taacataatt actaaataaa ttgcgacaat
      601 aaataaacct aaaatcaaaa ataaaatagg ctgtaaaaac tgagtctgtt ttatcgcttt
      661 atcttctatt tgttttacta atatttgcga atataacttt agttctactt ctagcttccc
      721 tctcttttca ccttgtagta caaacttaat taattgaggc ttaaagcatt ttagtttttc
      781 taaaatttga ggcaaaccat atcccatttc tgaataagtt aataagtatt tacctagaaa
      841 ctgtctaaat ggatcactac tatggtttat ataaacgtct actattgatt gaagtgtaat
      901 accattttta taaaacaaca ctaattcatt agttacaaaa taagttttaa ataattggaa
      961 atagcctgat attagcggta gtttcatcac aaagtttatc ttattgagca tatttaaatt
     1021 gttataaatt aatttcataa taatagccaa catagatact attatgagca ttactacaat
     1081 tatagtaggt aagctggtaa tgaaaaaaga caatgttttt tgaaaagaag atagttgaat
     1141 attcatagaa gtatataatt gttgaaactg tggaattact gtgaggttta atataataat
     1201 catagcaata aagatagaaa ctagtattaa ggggtattgc agtgtcttta acaatcgttg
     1261 ttctgacttt ctattcactt tcatataatt tacggtttct tctagaacgt ctataatatt
     1321 gccaaatctt tctgccaaat atacttgcat gacgatagta tcgctataac ctatcagtga
     1381 taatatttga ttgcatggtg caccatttga aatttcactt agaatggtgg tacctaattg
     1441 cttattttta tatgtcattt gaagatttaa aaattgaaaa ctttgataca gagtgaaacc
     1501 atatttcaac aaattacata aatttgaaag taagtcgatt tgttgggcct tacttaattg
     1561 tcgcttctta gaatgtagtt taaatgtatt tatccattgt agtttcacaa atgacacctg
     1621 cttttgtcat atcatcaagt ttatcttcta agttcttaaa tgatgatggt aatgaatgat
     1681 tatgggaaaa gaaatatcgg agttgttgct gagatagaat ttcacatact aattgtcgct
     1741 gttgcttaat agtagttaca agtcgttggt ttataattaa gttagttgcc tgtatcaatt
     1801 cttgtacaga aatgcccatt tctaatagcc ttaaaatagc acctttacaa tcagttgcat
     1861 gcaatgtagt cagaacaagg tgaccactta aactagcctg tataacacac ttggcaacat
     1921 ctttatctct gatttcacct attaaaataa catcaggatc acatcttaaa atagctttaa
     1981 acgaatttac atagttaatg ccagctttat cattcacatt aatttggacg ataccaggaa
     2041 tttgcatctc tacaggatcc tctatagaaa ttacatttaa attcaaggct ttattcgcgt
     2101 atgagaccat ttgatacatt aatgtactct ttcctgaacc agttggccca ctaaacaata
     2161 gtaatccttg tttcttattc atgaggtgtt taaaatcatt gaatttataa gttgatttct
     2221 gttgttgaaa aaattgaggt acaattctga taacacaact ttcttggcca agtgacaatg
     2281 gtaaagttga tattctcaaa aaatatattt tattgaaatg gtaactatat cgaccgctct
     2341 gtgcgacttg ctgtgtagaa acatcaagcc cagcttgaaa cttcatataa actaataact
     2401 tttgataaat gctattccca atttgttcat actgctccaa gttatcatta attctaaatt
     2461 taatacttac ttcattttta actggaataa aatgtacatc actcgcattc atttctatcg
     2521 ctttattaat tatttcttga aatagaatct tcaaaaaaac acctcctaca tataatcacg
     2581 taggaggtgt ttttattact tcaatttaac cgtgtaaaaa tggatttaat tgttcatcat
     2641 caaccgtcgt atatggacca tgtccaggga ataaaggtaa atcgccttct aattcaaata
     2701 ttttatcttg aatagaatca actagcgttt cataatcacc tttatataaa tctgtacgtc
     2761 cgattccatt attaaataat gtatctccaa caactgcgaa ttcatcgaac acatatgtta
     2821 aacttcctgg tgaatgtcca ggtgtgtgta acacattaaa cttaaatcct tctatttctg
     2881 tgctaccttc gtttaacttt tcaggagtta ccttacttgt aataattggt aatccatatt
     2941 gcttaaattt atctgcccca tttttaacgg gatcttttag aaaatcaaac tctgcttcat
     3001 gcatataaac cgggacatcg aatcgatcaa ctatatcatc gactgctccg atatgatcaa
     3061 agtgtgcatg tgttaataaa atagctttta acggtttatt tatttggttt aattttttaa
     3121 taattttttc actttcacct gaagggtcaa ttagaataac agctttgtca ttttcgatga
     3181 aatacgtatt agtatcaact aagcctaaag ttaagcttga aatcctcata ggttttcacc
     3241 actattttct aaatgttttt gtactgatgt tagtttatca ttcatttttc tagatttgtc
     3301 tcgtcggtca tcaatacgga tatttgtaca aactctactt aaacctttat caaaaggtaa
     3361 ttcatgtatc acttgcacaa cttctaatac atcgcttaat tcaccttcaa ttagagtatt
     3421 cattggtgtt aattgaaaat caattttacc cattgcttta tattcttgaa gttttttctg
     3481 aatatctgca atatatttac taacactcgg accttccgtt ccaactggaa taacaaccac
     3541 atcaacaata gccattattt taccccctct ttatctaata cataggtctt gattaatcct
     3601 gctgctcctg taatacctgc atcattacct aatttcgctt gtacaatttc agtttcaaat
     3661 tgagcaggtg taaatgttaa attatgatat tctgttttaa tattttcaat taaaataggt
     3721 cctgcagtag acattcctcc acctaaaacg atatatttcg gattacttgt aacactaata
     3781 atactacata aatatccaat atagtttgca accttttcag taatgaaaat acagaattgg
     3841 tcaccagctt ttgccgcatc aaaaacagct tttgctgtaa ccttattttc tttaatcaat
     3901 tctaatatag aagatctaaa cgtcaacttc ggatagtaga agttaactaa gttaacaacg
     3961 cctgtcgctg aagcaactgt ttcaatacat ccagaacgac cacaattaca tttaaatcgt
     4021 tgatcgaagt ctgctctaaa atgacctatt tctgcgccag agccattatg accatgtacg
     4081 atttcaccat ttgaaataat tcctccacct agacctgtac caagtgtgat ggcaacaaca
     4141 tcatcggcac cttcaccagc acctttgtgt ttctccccta aagcagctat gttagcatca
     4201 ttatctacat acactggaca atcaacgaat tgttcaaaaa tctcacgtac attaactttt
     4261 tctggccaat ataagtttac tgctccattt actgtacctt tttcaaagtc aacaggacct
     4321 ggtacaccaa tacctacgcc aagtacattt gaaaaattat aattattttc atttactttt
     4381 tcaacaaacg aatcataaat tcctttcaaa agtgtatatc ctgtactatc agatgtatca
     4441 gtgtgaatag accatttatg taattgttct aattcaggtg tgaaaatacc taatttacaa
     4501 gtcgtcccgc ctacatcagc tgctaaaata atttttgtca tttctgttca ttccttctct
     4561 gattaattat caacgtacat ttcaaatatt cttccttaga taacagttca tattgataca
     4621 atgatgaaat ctcctgttga atcatttcgt acatatcttc tggattttta aaatatatta
     4681 gaaatccgta acttttcaat aactgttgta catcataaaa gttatttatt tttcgactca
     4741 tttgatttag tcaactcttt ttctaagtta atataatctg tatttttagg gtcagcattt
     4801 aatgctttac gcacatattt taatgctttt tcatcatcat ttaatgaacg atttgctatc
     4861 gctaactcaa aatttaataa acctgattta ggaaacattc taagtcctcg ctcccattct
     4921 gtcatacctt cagacttaga attgatagta gccataatca taccacttaa ataatatgtt
     4981 tgatcatcgg cataat
//
LOCUS       DAHHYJ010000165         4784 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000165 DAHHYJ010000000
VERSION     DAHHYJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.166"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            144..806
                     /locus_tag="NBK22_002375"
     CDS             144..806
                     /locus_tag="NBK22_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2415533.1"
                     /translation="MLLEIKDLVYKASDRIILDHISLKVDKGESIAIIGPSGSGKSTF
                     QKQICNLISPTSGELYFKGKPYNDYDPEELRQRISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDRKRAKQLIKDVGLGHYQLSSEVENMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDVHNKEKIENIIFKLVEQDVSIMWITHSDDQSMRHFQKRITIVDGKISKVEELNQ
                     HE"
     gene            799..1575
                     /gene="fetB"
                     /locus_tag="NBK22_002376"
     CDS             799..1575
                     /gene="fetB"
                     /locus_tag="NBK22_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCD2415534.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLILCVLVIIINASWNTISRASPVMHHVFWISFLAIFIGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            1971..3158
                     /locus_tag="NBK22_002377"
     CDS             1971..3158
                     /locus_tag="NBK22_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2415535.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTF
                     KSS"
     gene            complement(3220..4572)
                     /locus_tag="NBK22_002378"
     CDS             complement(3220..4572)
                     /locus_tag="NBK22_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCD2415536.1"
                     /translation="MKINTTGGQIHGITQDGLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSTTFTEHEDCLYLNIWKQHNDQTKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKDFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYLDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MVFWFGNLQILAAHQYPTTAHLKFLSRQMQNDLANFAKSGKMPWPMYHNERRYYRTYQ
                     "
BASE COUNT         1507 a          716 c          915 g         1646 t
ORIGIN      
        1 tttgttttta atttaaatgc tgaaaatcaa ttatgcctaa attttgatat tacaagaaaa
       61 tgattttttc ttaaatgtaa ttgcactaaa aaccaaaaaa acgggaataa tatacctgat
      121 atattacatg aggagcggtg caaatgttgt tagaaattaa agatttagtg tataaagcga
      181 gcgatagaat catactagat catatcagtc taaaagtaga taaaggcgag agcattgcca
      241 ttataggtcc atcaggtagt ggtaaaagta catttcaaaa gcaaatatgt aatttgatta
      301 gtccaactag tggagaactt tattttaaag gtaaacccta taatgattat gacccggaag
      361 aattgcgtca acgaatcagt tatttgatgc agcaaagtga cttgtttggt gaaacgattg
      421 aagataacat gatattccca tcacttgcac gtaatgataa atttgataga aaacgtgcaa
      481 agcaattaat taaagatgtc ggtttgggac attatcaatt aagttcggaa gtggaaaata
      541 tgtcgggtgg tgagcggcaa agaattgcta tagcgcgcca actgatgtat acaccggata
      601 ttcttttatt agatgaatcg accagtgcat tagacgttca taataaagaa aagatagaaa
      661 atatcatttt taagttagtg gaacaggatg tttctatcat gtggattacc catagtgatg
      721 atcaaagtat gagacatttt caaaagcgca taacgattgt tgatggcaaa atttctaagg
      781 ttgaggagtt gaatcaacat gagtaacacg gcacttggat tgacggcatt gcttttagtc
      841 attccgatta tcatttcata taaagaaggt ttacatatta ttaaagattt aattgttgcg
      901 acattacgcg cagttgtgca attaatcatt ttgggatttt tgctgcatta tatttttaaa
      961 ataaacgata aatggctgct tattttatgt gtattggtca ttattattaa tgcatcatgg
     1021 aatacaatta gtcgagcatc accagtgatg catcatgtgt tttggatatc atttctagct
     1081 atcttcattg gaacggcatt accgcttgca ggtactattg cgacaggggc cattcaattt
     1141 acagccaatg aagttatacc tattggcggc atgcttgcaa ataatggctt gattgcaatt
     1201 aatttagctt accagaattt agatcgtgca ttcgtacaag atggtactaa tattgaatct
     1261 aaattatcac ttgcagctac acctaaattg gcttctaaag gtgcaatacg tgaaagtatt
     1321 cgtttagcta tagtgccaac aattgattcg gttaaaacat atgggcttgt gtcgattcct
     1381 ggtatgatga caggcttaat tattggtggc gtaccacctt tacaagcgat taaatttcaa
     1441 ttgttagtcg tgtttattca tacaactgcg accattatgt ctgctttgat tgcgacatat
     1501 ttaagctatg gtcaattttt caatgcaaga catcaattag tagcacgaaa tactgatgtt
     1561 aagagtgaat catgatagat tttactgcat cagatttagg cattagtttt aattggaaat
     1621 gaagtgacgc gcacatatag tatcgctatt cattaacaca gcgaaaatat tcataaaggc
     1681 acgcatactt tgtagtcagt tatctgttct gacatataaa gcgtgcgtgc ttttttggag
     1741 tcattgttga aactgaagta attatacata attattaaat gacatacttg tgttaatttt
     1801 tcaaatactg aaaaacaatt tcaataattt tccgattaag cacagaaaat taaagcaaaa
     1861 tattatataa tagaacggtt atatataaaa ttgtgttgca cacatttttt aataaatcgt
     1921 tattctaagg gaaatgaata tcggaaattt tgtttgaaag gagttttaaa atgtcaatca
     1981 tgcgactatt tacattcatt ttaagtattt ttatcgtagg aatggttgaa atgatggttg
     2041 caggaattat gaacttgatg agtcaggact tacatgtatc agaagctgtc gttggtcaat
     2101 tagtgacaat gtacgcttta acatttgcga tatgtggacc tattctggtc aaattaacga
     2161 accgtttttc atcaaggcct gtattattat ggacattact tatatttatc attggtaatg
     2221 gcattattgc tgtagcgcca aatttttcaa tattagtagt tggtagaatt atctcatctg
     2281 cagcagcagc actaattatc gtaaaagtat tagctattac agcgatgtta tcagcaccta
     2341 aaaatcgtgg taaaatgatt ggacttgtct atacagggtt tagtggggcc aatgtttttg
     2401 gtgtaccaat tggaacggtt atcggcgatt tagtaggttg gcgctataca tttctattct
     2461 taattattgt gagtattatt gttggcttct tgatgatgat ctatttaccg aaggatcagg
     2521 aaatacaacg aggccctgtg aatcatgagg caccatctca tgaaaatcat gttacttcga
     2581 aaatattaag acctgctgaa gtagcaaaat atttaattat tacgttttta gtattgattg
     2641 caaactcagt gacattcgtc tttataaatc cacttatttt atctaatggt catgatatgt
     2701 cattcgtttc attagcactt ctagtaaatg gaatcgctgg cgttattgga acatcattgg
     2761 gtggtatatt ctccgataaa attacaagta agcgttggtt aatgatttct gtttctattt
     2821 ttatcgttat gatgttactt atgaatttaa tcttacctgg ttcaggtcta ttgttagcag
     2881 gactatttat ttggaatatc atgcaatgga gtactaatcc agcagtgcaa agcggtgtga
     2941 ttcaacatgt tgaaggcgac acaagccaag taatgagttg gaacatgtct agtttaaacg
     3001 ctggtattgg tgttggaggc attattggag gcttggtcat gacacatgtt tctgttcaag
     3061 ctatcacata tacgagtgcc atcattggcg cattaggatt aatcgttgtt ttcacattga
     3121 aaaataatca ttatgctaaa acatttaaat catcataatt ctcttatgaa aagcacgcct
     3181 gctatcaaat tcaggtgtgc ttttttagat gcgataacgt tattgatatg tgcgataata
     3241 gcgacgttca ttatgataca tcggccaagg cattttaccg cttttagcaa aattagctaa
     3301 atcattttgc atttgtcgac ttaaaaattt aaggtgagca gttgttggat attgatgtgc
     3361 tgctaatatt tgtaagtttc cgaaccaaaa taccatatct agaatatggt aagcactgcg
     3421 atagtgtgcg ctagaagtat catgccatgc aaattcagca agccatagtt gtgcgtttga
     3481 atcttgtata ttaagttgtt gtaaaaattg cttcatcggc tgtttgaaat aacaatgtgt
     3541 aatcgctaaa gattgttgct tcgcagtctg aacatcttcg tattttaaag gaatatcatt
     3601 taattccata atgtcgataa agcgttgcgg cgataatttt ttctgttcac tagtaatata
     3661 aatatcgcct tcatcttttg tataacatgc aaaaattggt ttcgttgttg gataattatt
     3721 ttgtatataa tctgttttaa taggcgcata tattaaatca agccctttag aaggtcctcg
     3781 agattgtttt agcttcgcca tcagcataag aatatcatct gtcgataatg atgtaacatc
     3841 atctgtatct aaataatcga gcatcatttt ttggaaatgt tgtgctttat tgcgtgcact
     3901 ctcaagggtg tctaatcgta gtgcgccact tagtagaacg actttatgga agtatggctc
     3961 aatgtcaggt attttaagta aagtcaaaat gctcatactg cctgcagact gacccattaa
     4021 agtaatgtta ttagcgtcgc caccgaagga ttcaataaat tgatgcaccc attttatgac
     4081 attgatttga tctgaaaggc cattattgga atgaaaatct ttattaaaat atgaccagtc
     4141 taaatatcct aatgcgccta aacgataatt gcatgtaata acgataatgt cgttattttg
     4201 tactaaatgt gccggttgat agagttcggc tgtaccatga ccattttcaa aactaccacc
     4261 ataaaaataa atgatgacag gtttcttcgt ctgatcatta tgttgtttcc aaatatttag
     4321 atataaacag tcttcatgtt cagtaaaagt tgtagattgt gaggagaaaa aatcttctaa
     4381 tttgttgtct ggttgcggtg ggatgggttg tatttcagtt gcatcaattg gctctgacca
     4441 ttgtgttttt aacgtagaat gtttaaagcg attgtcatga actggtggtt ctgcataagg
     4501 aatgcctaag aagatatcta aaccatcttg tgtaatacca tgaatttgac cacctgtagt
     4561 attaatcttc atatttatta acctccttta tcatccccaa tttaatcata ccacggtcta
     4621 tagatgatag gtgacaagta taacaagaca attttgtgac tgaatcagac tagcttgata
     4681 tctgttttaa ctcaaaccat tttcagcatt atatgatgtt cttctacggg agtgggacag
     4741 aaatgataaa gagccactaa tgatttatta tgtagtggtt ctta
//
LOCUS       DAHHYJ010000166         4524 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000166 DAHHYJ010000000
VERSION     DAHHYJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.168"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            36..596
                     /locus_tag="NBK22_002379"
     CDS             36..596
                     /locus_tag="NBK22_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD2415537.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            649..1578
                     /locus_tag="NBK22_002380"
     CDS             649..1578
                     /locus_tag="NBK22_002380"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCD2415538.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(1773..1946)
                     /locus_tag="NBK22_002381"
     CDS             complement(1773..1946)
                     /locus_tag="NBK22_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415539.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            1998..3377
                     /locus_tag="NBK22_002382"
     CDS             1998..3377
                     /locus_tag="NBK22_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCD2415540.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKIARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSEQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(3497..>4524)
                     /locus_tag="NBK22_002383"
     CDS             complement(3497..>4524)
                     /locus_tag="NBK22_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCD2415541.1"
                     /translation="TNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYLV
                     RNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFEA
                     VYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAVT
                     DLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAEY
                     NDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQHL
                     ELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVAS
                     EGVCVIF"
BASE COUNT         1554 a          762 c          710 g         1498 t
ORIGIN      
        1 gagtttattt tattattata taggagagat gttgaatgac acatcgcgca ctattagttg
       61 ttgactattc atatgacttt atcgcagacg acggcttact aacatgcggt aaacctggac
      121 aaaatattga agattttatt gtttctcgta tcaatgactt taattattat caagaccata
      181 tattcttttt aatggattta cattatttac atgacattca tcatcctgaa agtaaattat
      241 tcccaccaca caatatcgta gatacaagtg gtagagaatt atacggtaaa gtaggtaaat
      301 tatacgaaac aattaaagcg caacctaatg tacatttcat tgataaaacg cgctatgatt
      361 cgttctttgg taccccgctt gatagtttat tgagagaaag aagtattaat caagtcgaaa
      421 tcgttggtgt atgtaccgat atttgcgtgt tacatacagc aatttctgca tacaacttag
      481 gttataaaat ttcagtacct gctgagggag tggcttcatt taatcaaaaa gggcatgaat
      541 gggcacttgc acatttcaaa aactcattag gtgcagaggt agaacaacac gtttaaatcg
      601 tgctaaaata attataaaga atacaattta caagggagat atttgacaat ggctaaaaca
      661 tatattttcg gacataagaa tccagacact gatgcaattt catctgcgat tattatggca
      721 gaatttgaac aacttagagg taattcagga gccaaagcat accgtttagg tgatgtgagt
      781 gcagaaactc aattcgcgtt agatacattt aatgtacctg ctccggaatt attaacggat
      841 gatttagatg gtcaagatgt tatcttagtt gatcataacg aattccaaca aagttctgat
      901 acgattgcct ctgctacaat taagcatgta attgatcatc acagaattgc aaatttcgaa
      961 actgctggtc ctttatgtta tcgtgctgaa ccagttggtt gtacagctac aattttatac
     1021 aaaatgttta gagaacgtgg ctttgaaatt aaacctgaaa ttgccggttt aatgttatca
     1081 gcaattatct cagatagctt acttttcaaa tcaccaacat gtacacaaca agatgttaaa
     1141 gcagctgaag aattaaaaga tattgctaaa gttgatattc aaaagtacgg cttagatatg
     1201 ttaaaagcag gtgcttcaac aactgataaa tcagttgaat tcttattaaa catggatgct
     1261 aaatcattta ctatgggtga ctatgtgact cgtattgcac aagttaatgc tgttgacctt
     1321 gacgaagtgt taaatcgtaa agaagattta gaaaaagaaa tgttagctgt aagtgcacaa
     1381 gaaaaatatg acttatttgt acttgttgtt actgacatca ttaatagtga ttctaaaatt
     1441 ttagttgtag gtgctgaaaa agataaagtc ggcgaagcat tcaatgttca attagaagat
     1501 gacatggcct tcttatctgg tgttgtttct cgaaaaaaac aaatcgtacc tcaaatcact
     1561 gaagcattaa caaaataata ctatattact gtctaatcat agacatgttg tatttaacta
     1621 acagttcatt aaagtagaat ttatttcact ttcaatgaac tgttttttat ttacgtttga
     1681 ctaatttaca accccttttc aatagtagtt tttattccct tagctaccct aacccacaga
     1741 ttagtgattt ctatacaatt ccccttttgt ctttacattt tcttaaaata tttgcgatgt
     1801 tgagtataaa tttttgtttt cttcctacct ttttcgttat gattaaagtt ataaatatta
     1861 ttatgtacac gattcatcgc tctattttca actttcaaca tatataattc gaaagaccat
     1921 ttaaaattaa cggccacaac attcaaatca attaatcgct ttttccaaaa taatcatata
     1981 aggaggttct tttcattatg aatatcattg agcaaaaatt ttatgacagt aaagcttttt
     2041 tcaatacaca acaaactaaa gatattagtt ttagaaaaga gcagttaaag aagttaagca
     2101 aagctattaa atcatacgag agcgatattt tagaagcact atatacagat ttaggaaaaa
     2161 ataaagtcga agcttatgct actgaaattg gcataacttt gaaaagtatc aaaattgccc
     2221 gtaaggaact taaaaactgg actaaaacaa aaaatgtaga cacaccttta tatttatttc
     2281 caacaaaaag ctatatcaaa aaagaacctt atggaacagt tttgatcatt gcaccattta
     2341 actatccttt tcaactagtg ttcgaacctt taatcggtgc tattgcagca ggtaatacag
     2401 caattattaa accatctgag ttgacaccaa atgttgcacg agtgattaaa cgattaatca
     2461 atgaaacatt tgatgcaaat tacattgaag ttattgaggg aggaattgaa gaaacgcaaa
     2521 cgttaattca cttacctttt gactatgtct tctttacagg aagtgaaaat gtaggcaaaa
     2581 tcgtttatca agctgccagc gaaaatttag ttcctgtgac attagaaatg ggcggtaaat
     2641 ctccagtcat cgttgatgaa acagcgaata ttaaagttgc tagtgagcgc atttgttttg
     2701 ggaaattcac taatgccggc caaacatgtg ttgcaccaga ttacatttta gtacacgaat
     2761 ctgtaaaaga tgatttaatc acagccctat caaaaacgtt gcgtgaattt tatggtcaaa
     2821 atatacaaca aagtccagat tatggccgca ttgtaaacct taaacattat catcgtctga
     2881 cttcattact taacagtgaa caaatgaata ttgtatttgg tggtcatagt gatgaggatg
     2941 aacgttatat agaaccaaca ttgttagatc acgttacaag tgattcagca attatgcaag
     3001 aagaaatttt tggtcctatc ttaccgattt taacgtatca gtcattggat gaagcaatag
     3061 cctttattca ccaaagacca aaacctttga gtttatattt atttagcgaa gatgaaaatg
     3121 ctacacaacg tgtaataaac gagctatcat ttggcggcgg cgctattaat gatacattga
     3181 tgcacctagc gaatcctaaa ttaccatttg gtggtgttgg cgcctcaggt atgggacgct
     3241 atcatggtaa atattcattc gacactttta cacatgaaaa aagctacatt ttcaaatcta
     3301 cacgattaga atcaggtgtc catttaccac catataaagg taaatttaaa tacatcaaag
     3361 ctttctttaa aaattaattt ttgagacaaa aataaaccat acttccataa tttgatgttc
     3421 ttgaacacaa taaatttcaa ggtatacata aattttttgg aaacatggtt ttctttttat
     3481 catgattaat taaagattaa aatatgacac atacaccttc agatgctaca cgagtgttat
     3541 cacatagcgt aattttacct gtttctttat ttctttcgaa aacagttaca actgaatctc
     3601 cttgctcatg agcacaaact aaaaggtcat ctgatgaggc aatattaaaa tctcttggga
     3661 attgaccacc actttcagta attgttacta gttctaagtg ttgaccatta tcaagaactt
     3721 taaaaattgc aatgctatca tgccctctat tagatacata taagaattgt tgatcatgag
     3781 ataaacgcac tgctgcaagt ttagtatctc catcaaagtt ttcaggaatt gttaaatgac
     3841 gctcgagctc ttcaaattta ccgtcattat attctgcaac actcacagta tttgataatt
     3901 cgtgtacgac ataagcaaat tttccattat catgaaattc aatatgtctt gtcccatcac
     3961 tatctttaaa taaagattct ttataaaatt caaacccgtt gtcatcaaat ttataagtaa
     4021 cgatacgatc agcacctaaa tctgttactg caacgtactt accatctgga gtttgattaa
     4081 tataatgtgc gtgtggatga tcttgtcttt catgtgtacc tgttggaaaa tcatgtgcaa
     4141 gttcttgaat tagacgtata atttcacctg tgtgcgtatt taattcatac atacgtatga
     4201 tgccagcacc atataccgct tcaaataaat atcgtttatc ttctgaaatc gatacataac
     4261 aacctgtacc agcttttgaa gacaaacatt tatttaataa atgcaattca ccattgtcat
     4321 caattttcaa acttgcaaca ccgcattgtt ctccttcttt gttgattcca tataaaactt
     4381 cattattacg caccaaatat gtagacgctt ctaattcaaa tcctgtttct aataaatcaa
     4441 tacgtgactg attttcgttt aattcaaaac gataaatccc tttaccattc tttttagtgt
     4501 aagaaccaat atatccattt gtca
//
LOCUS       DAHHYJ010000167         4508 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000167 DAHHYJ010000000
VERSION     DAHHYJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.169"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(89..619)
                     /locus_tag="NBK22_002384"
     CDS             complement(89..619)
                     /locus_tag="NBK22_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD2415542.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNTLELVAVLQNEVVGHGLLSEVYLDNEAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            complement(758..1279)
                     /locus_tag="NBK22_002385"
     CDS             complement(758..1279)
                     /locus_tag="NBK22_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415543.1"
                     /translation="MINIISAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQI
                     LLEREEDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGKDKVIQKKYVHIL
                     PSKEGYMLPINKNVYEELERTIENNGHEADLNVRMTYYHNVSRKQQEVILKGQIDRFN
                     TYNNKEIYDLQFI"
     gene            complement(1287..1442)
                     /locus_tag="NBK22_002386"
     CDS             complement(1287..1442)
                     /locus_tag="NBK22_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415544.1"
                     /translation="MMVKKLLKAIKQNFLYEQSNITFKMIVFGYKRLSKMLFQFEINH
                     INFLWEG"
     gene            1877..2593
                     /locus_tag="NBK22_002387"
     CDS             1877..2593
                     /locus_tag="NBK22_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCD2415545.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELKE
                     FKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDKDLNP
                     SSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFIYELR
                     GDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEYKKEQ
                     EREKEKEKQKDDDHSGLDEV"
     gene            2678..2931
                     /locus_tag="NBK22_002388"
                     /pseudo
     CDS             2678..2931
                     /locus_tag="NBK22_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2947..3270
                     /locus_tag="NBK22_002389"
     CDS             2947..3270
                     /locus_tag="NBK22_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415546.1"
                     /translation="MNNEELEMRLLLMKQSIEQLQEELAPNLKTRDLVLLRYMYSYKE
                     INMLDSYLFQLATNKEQITKKQFKTKLENIREVPEIPIRQVNDILEGYKNSELYVELI
                     NSILK"
BASE COUNT         1721 a          663 c          602 g         1522 t
ORIGIN      
        1 taagaattac aaacgacaac gtacgatttg attgcaaaca ttttttatta ttaatatgaa
       61 ctctacctaa tgtaatccta gcttgaaatc atattttttc aaaagcagat gtgtaattta
      121 tggtacctgt ttttcccgct aaactgttca cttttaattc tttaattaaa aacgcttcgt
      181 ctggtatacc atcatatggt ggataaatgt cgtacatact tgcgcgttga tatcctagat
      241 tggcataata cgtcggccat cctaatacac tgataaaatt atatcctttt aatattgctt
      301 ctcgttctaa tgcttgaatc aatcgcttcc caataccttt attttgatga tgaatatcaa
      361 cagatacagg tgctaacact aatccaattt cccgttgtgc ctcgttatca agataaactt
      421 cacttagtaa accgtgccct acaacttcat tttgaagaac agctactaat tctaaggtat
      481 tgtcatacgt atcacttaga cgaatttggt ctactagctc tgattcatta ccataaccat
      541 gttcactatt ttcaaatgcc gttctaatta actggtccac ttgagcaaag tcatgttgat
      601 gtatttgtct aatttgcatc tttgcccctc cttttagttc tatcttatag aaaaatgcat
      661 tacaacataa agtaatattt tatagtaatg acgccattat ttcattaaaa ttgaatgata
      721 tttttcgcaa taaacaacta ccctcttaaa tcaattttta gataaactgc aaatcataaa
      781 tttctttatt attataagta ttaaaacggt cgatttgacc ttttaatata acttcctgtt
      841 gtttgcgact tacattatga taataagtca tacgtacatt caaatcagct tcatgaccat
      901 tgttctcaat cgttctttct aattcttcgt acacattttt attaattggt aacatgtacc
      961 cttctttact aggtaaaata tgcacgtatt ttttctgaat gactttatcc ttccccaata
     1021 aaacctctat tgcaattttt ttagcagcac cgccacctaa attatatagt ttaatataat
     1081 ctttatgatg atgctcttct gttgccgaat tcattatatt aagttgatcc tcctcccttt
     1141 ctaataaaat ttggttaaaa cctaaagctg gcagaaagct aattttcatt tgataaagtt
     1201 gaactgaaac tgatacaaaa taaaataaag ccataataaa tgttccaata gatcctatag
     1261 ctgaaataat gttaatcatt aagatatcaa ccctcccata agaaatttat atgattaatt
     1321 tcaaactgaa aaagcatttt acttaatcgt ttatatccaa aaacaatcat tttaaacgtt
     1381 atattcgact gctcatataa aaagttttgc tttatagcct taagtaattt tttaaccatc
     1441 aagtacgatt attattacgg caatctattt gtattgacta aaaaatacaa taacgctttt
     1501 aagagctccc atctttgacc agtattttcc aaattaataa cacgtcttct tcaatacata
     1561 tttatctcat acaaataagt tgattttttt atttttacaa acgtaatttc ttaacaaatc
     1621 aaaattttac atttaaatct ttcgatatat tacaagtttt ttaaaaaagt aatatatttc
     1681 tatcaataaa agtaatataa tattagtaat tatagtttca ttaatttatt gcaacaatac
     1741 ataggatcat tattacaaga attatttaat atcatcttag aatctcacaa aatatcaact
     1801 ttgtttaatt actcaaaatg ataagtcatt tagagttttt aaaataaatt tttgcgaaat
     1861 aaaggagaca tgttaaatga aaaaagtaat ggggatatta ttagcaagta cacttatctt
     1921 aggtgcttgt ggacatcatc aagatagtgc aaaaaaagag agcactagtc acaaaaagaa
     1981 agaaaatgac aatgaagaac ttaaagaatt taaaagcaaa aaaaatatgg atataaaaat
     2041 taaaggcgat actattgtta gtgacaaatt tgaagctaaa ataaaagaac cgtttatcat
     2101 caatgaaaaa gatgagaaaa agaaatatat cgcttttaaa atggaaatta ctgctaaaaa
     2161 agacgataaa gatttaaatc catcttctat ttctcatgac tatattaata tcactcaaga
     2221 tgataaaaat acagtaaata aattaagaga tggttatctt ttaagtgata aaaaatataa
     2281 agattggaca gaacataacc aagatcaaat taaaaaaggc aaaactgcac aagccatgtt
     2341 tatctatgag ttaagaggtg atggaaatat taatttaaat gtccataaat actcagaaga
     2401 taaaacagtt gattctaaat cattcaaatt tagtaaactt aaaaccgaag atttttctca
     2461 tagagcggaa acaagagaag aagtagaaaa gaaagaaaaa gaatttgaag aagagtacaa
     2521 aaaagaacaa gaacgagaga aagaaaaaga aaagcaaaaa gatgacgacc acagtggttt
     2581 agatgaagta taaatttggt ttgttgattg tcttacattc agttatttaa tttttagaga
     2641 aactgactta tttatagctt agccaacatg atactataaa gctatgatta aaaataaaaa
     2701 atctgtgttt aatggtgtta aagaaatcag tccccgtgcc ggaaaatcaa aaaaaaaaga
     2761 tgggataaat ttgtaaatcc cgcattgaat aatgcattaa aacaagaaga ggctacatgg
     2821 ggaaatgtac aaggtcaagt ttcacaagca ttgatgggaa ctggtattaa agattctact
     2881 gcaagaagca tagggttttg ggtttcccaa gttggtcaag cattaatata aaaagagtgt
     2941 gataaaatga ataatgaaga attagaaatg agattgttat taatgaaaca atcaatagaa
     3001 caattacaag aagaactagc gcctaactta aaaactagag atttagtgtt attaagatat
     3061 atgtattcat ataaagaaat taatatgcta gattcttact tgttccaatt ggctacaaat
     3121 aaagagcaaa taacaaaaaa acaatttaaa acaaaattgg aaaacattag agaagtacca
     3181 gaaataccta ttagacaagt taatgatata ttagaaggtt ataaaaatag tgagttatat
     3241 gttgagttga taaatagcat cctaaaataa aaataaacat actaagatta gctatgaagg
     3301 aatctatgac gatagatttt ttcatagcta ttttttatag ttataaagag gagtagtctg
     3361 ttctgactct tggattttaa atatgtataa taaacaagta agctttactc tcgcctttta
     3421 aaattcgttt gtagtatgtt gggttcttaa aatcgtgaat aggaaaatgc aatgagaaag
     3481 gttaagtaaa gtttttaact tctcatttat tcaatggaaa attttttatc gattacttag
     3541 acattaatat tagtaaaaga agtagtgtag ttgcaaatta taaaaactaa aaattccaaa
     3601 aagagtttat catccaaaac aataaaaatg gctacaatgg tatgttgtga aatgcatttc
     3661 ttaactacga cttcttttat ttccgtctcg tcaactattg ctgattgtgt taataggcat
     3721 attgattcta atgaaaatat caaaactcgg ctcatctctt tataactaca cccacttagt
     3781 gacgtaacaa aaatgaaaag ttctttttca gtagccattt atccaattaa gtgtagacga
     3841 ttcacatttt tatattgaca tatataacat ggagaatttt ttgcgtataa agaagtttct
     3901 tcatgaacaa tgttgaatgt aaaactcaga atccaacttt tttgacactt atttgacaca
     3961 catcactgtc cccttatatc tcaaaaacta attcataatc acttattact ctttcctcca
     4021 tcaaataaac acctatacaa acactgctat atcaatactc acaacaaatt ctgcaatgca
     4081 ttatcccctg aaaaacacat aataaaaaag cccacatcca caaaagttgt aggctacaaa
     4141 tatggagacg gcgggagttc attaaagtgt ttatctattc atgtataact cgctataagc
     4201 attgtcatat caacgtttaa attggctact ctattgtgtg taaaatgtta aaattgataa
     4261 aactatataa atttgtgcac ccaacatcac ctcataatta acgtattcaa atacgtattc
     4321 actgcctgca acgtgtggga tgatacatat ttttctataa ctcttccttt ataacctttt
     4381 gtatttttta tgaattttct aaaatttatt attcaactta tatttataat gttattataa
     4441 atataatgaa aaggaagtgc ctaatatgaa aaaaagatta ttatttgtaa ttgttattac
     4501 tttattta
//
LOCUS       DAHHYJ010000168         4486 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000168 DAHHYJ010000000
VERSION     DAHHYJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.170"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            581..2830
                     /gene="pflB"
                     /locus_tag="NBK22_002390"
     CDS             581..2830
                     /gene="pflB"
                     /locus_tag="NBK22_002390"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCD2415547.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            2853..3608
                     /gene="pflA"
                     /locus_tag="NBK22_002391"
     CDS             2853..3608
                     /gene="pflA"
                     /locus_tag="NBK22_002391"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCD2415548.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            3944..>4486
                     /locus_tag="NBK22_002392"
     CDS             3944..>4486
                     /locus_tag="NBK22_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCD2415549.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWTEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVF"
BASE COUNT         1583 a          691 c          837 g         1375 t
ORIGIN      
        1 catcaattct atgtatattt taatacaata attttagcaa taaatgacgc ataagtaatg
       61 ttaaatattt agaaatgttt atagatgact tgttaagacg ttgcaaatgt tgtgatagca
      121 caaaattttt gtttgtcaag acgatttacc gaggctgtaa aatcaaactg ttatatttta
      181 tttgtagctg ttatataaaa aaaggcaaga tattgaacgg ttcaaaagtg aatttttacg
      241 tcaataaaag tatttaatcc agtctcttca tatataaaag taaatctttc taagtgttga
      301 tttaacgctt atcaacaatc attttttata aacaaatata tactcctaaa ttaactttta
      361 aagcaatgaa aatagtgaac attataactg ttgtgtaaca gaatgcaatt agcatattac
      421 tgttacacaa attagtacag tttctatgtt ttgacataca tttgatgaaa attgtacata
      481 atttatgtga aaaaaatcac aacaaacatg ctacaatgac tatgaaaacg ttaacatagc
      541 atttcaaatt cacaacatta tacagatgga ggcgtttagt atgttagaaa caaataaaaa
      601 tcatgcaaca gcttggcaag gatttaaaaa tggaagatgg aacagacacg tagatgtaag
      661 agagtttatc caattaaact acactcttta tgaaggtaat gattcatttt tagcaggacc
      721 aacagaagca acttctaaac tttgggaaca agtaatgcag ttatcgaaag aagaacgtga
      781 acgtggcggc atgtgggata tggacacgaa agtagcttca acaatcacat ctcatgatgc
      841 tggttattta gacaaagatt tagaaacaat tgtaggtgta caaactgaaa agccattcaa
      901 acgttcaatg caaccattcg gtggtattcg tatggcgaaa gcagcttgtg aagcttacgg
      961 ttacgaatta gacgaagaaa ctgaaaaaat ctttacagat tatcgtaaaa cacataacca
     1021 aggtgtattc gatgcatatt ctagagaaat gttgaactgc cgtaaagcag gtgtaatcac
     1081 tggtttacct gatgcatacg gacgtggacg tattatcggt gactatcgtc gtgtagcttt
     1141 atatggtgta gatttcttaa tggaagaaaa aatgcacgac ttcaacacga tgtctacaga
     1201 aatgtcagaa gatgtaattc gtttacgtga agaattatca gaacaatatc gtgcattaaa
     1261 agaattaaaa gaacttggac aaaaatatgg tttcgattta agccgtccag cagaaaactt
     1321 caaagaagca gttcaatggt tatacttagc ataccttgct gcaattaaag aacaaaatgg
     1381 tgcagcaatg agtttaggtc gtacatcaac attcttagat atctatgctg aacgtgacct
     1441 taaagcaggc gttattactg aaagcgaagt tcaagaaatt attgaccatt tcatcatgaa
     1501 attacgtatt gttaaatttg ctcgtacacc tgattacaat gaattattct ctggagaccc
     1561 aacttgggta actgaatcta tcggtggtgt aggtattgac ggacgtccac ttgttacgaa
     1621 aaactcattc cgtttcttac actcattaga taacttaggt ccagctccag aaccaaactt
     1681 aacagtatta tggtcagtac gtttacctga caacttcaaa acatactgtg caaaaatgag
     1741 tattaaaaca agttctatcc aatatgaaaa tgatgacatt atgcgtgaaa gctatggcga
     1801 tgactatggt atcgcatgtt gtgtatcagc gatgacaatt ggtaaacaaa tgcaattctt
     1861 cggtgcacgt gcgaacttag ctaaaacatt actttacgct atcaatggtg gtaaagatga
     1921 aaaatctggt gcacaagttg gtccaaactt cgaaggtatt aacagcgaag tattagaata
     1981 tgacgaagta ttcaagaaat ttgatcaaat gatggattgg ctagcaggtg tttacattaa
     2041 ctcattaaat gttattcact acatgcacga taaatacagt tatgaacgta ttgaaatggc
     2101 attacatgat acagaaattg tacgtacaat ggcaacaggt atcgctggtt tatcagtagc
     2161 agctgactca ttatctgcaa ttaaatatgc acaagttaaa ccaattcgta acgaagaagg
     2221 tcttgtagta gactttgaaa tcgaaggcga cttccctaaa tacggtaaca atgacgaccg
     2281 tgtagatgat atcgcagttg atttagtaga acgctttatg actaaattac gtagtcataa
     2341 aacatatcgt gattcagaac atacaatgag tgtattaaca attacttcaa acgttgtata
     2401 cggtaagaaa actggtaaca caccagacgg acgtaaagct ggcgaaccat ttgcaccagg
     2461 tgcaaaccca atgcatggcc gtgaccaaaa aggtgcatta tcttcattaa gttctgtagc
     2521 taagatccct tacgattgct gtaaagatgg tatttcaaat acattcagta tcgtaccaaa
     2581 atcattaggt aaagaaccag aagatcaaaa ccgtaactta actagtatgt tagatggtta
     2641 cgcaatgcaa tgtggtcacc acttaaatat taacgtattt aaccgtgaaa cattaataga
     2701 tgcaatggaa catccagaag aatatccaca gttaacaatc cgtgtatctg gttacgctgt
     2761 taacttcatt aaattaacac gtgaacaaca attagatgta atttctcgta cattccatga
     2821 aagtatgtaa caaaatttaa ggtgggagca ctatgcttaa gggacactta cattctgtcg
     2881 aaagtttagg tactgtcgat ggaccgggat taagatatat attatttaca caaggatgct
     2941 tacttagatg cttgtattgc cacaatccag atacttggaa aattagtgag ccatcaagag
     3001 aagtcacagt tgatgaaatg gtgaatgaaa tattaccata caaaccatac tttgatgcat
     3061 cgggtggcgg tgtaacagtc agtggtggcg aaccattgtt acaaatgcca ttcttagaaa
     3121 aattatttgc ggaattaaaa gaaaatggtg tgcacacttg cttagacaca tcggctggat
     3181 gtgctaatga tacaaaagca tttcaaaggc attttgaaga attacaaaaa catacagact
     3241 tgatattatt agatataaaa catattgata atgacaaaca tattagattg acaggaaagc
     3301 ctaatacaca catccttaac ttcgcgcgca aactgtcaga tatgaaacaa cctgtatgga
     3361 ttcgacatgt ccttgtgcct ggttattctg atgataaaga cgatttaatt aaactagggg
     3421 aattcattaa ttctcttgat aacgtcgaaa agtttgaaat tctgccatat catcagttag
     3481 gtgttcataa gtggaaaaca ttgggcattg catatgaatt agaagatgtc gaagcgcccg
     3541 atgatgaagc tgttaaagca gcctaccgtt atgttaactt caaagggaaa attcccgttg
     3601 aattataaat acaattcaga ccgaaaagaa agcatatgta acttcaagag tgaaggggca
     3661 tatgcttctt tttcaattga gtattgagta ttagcaagac gtagtaggta tatgagacaa
     3721 cttctacaat ggttgaagga agacgttttt gtaagtagct atgctgataa agaatgtgat
     3781 gtcttgttaa aggtggggtt ccaatatcat catttagctg atgttgaatg ggttattatt
     3841 tgctacttgc atatgaatat gagtcttttc aaatttttat tgaccctgag taatgaaaaa
     3901 tattaagatg aaacttaata ttaaagcaat gcggagcgtg attatgaaga gaattagtaa
     3961 agatatatgg gcagtattta aattactgta tcaaaataaa gggcgtttta gcattaatgc
     4021 cttactattg cagttaatca tgatttttat tagtagtaca tacttaattt tactatttaa
     4081 tatgatgtta aaagtagctg ggcaaagcca acttacgatt aacaattgga cggaaatcgt
     4141 tagtcatccc gccagtgtga tacttcttat tatattcata ttaagtgttg cctttctgat
     4201 ttatgtagag ttttcattgt tagtttatat ggtttatgcc ggctttgatc gacagattat
     4261 tacatttaaa tccattttta aaaatgcctt tgtaaatgtg cgtaaactca taggtgtacc
     4321 agttattttc tttgtcattt atttaatgtt aatgataccc attgccaacc taggactaag
     4381 ttcagtatta acaaaaaata tttacatacc taaattttta acggaagaac ttatgaaaac
     4441 gacgaaaggt ataatcattt acggtacctt tatgattgct gtattt
//
LOCUS       DAHHYJ010000169         4475 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000169 DAHHYJ010000000
VERSION     DAHHYJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.171"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(287..994)
                     /locus_tag="NBK22_002393"
     CDS             complement(287..994)
                     /locus_tag="NBK22_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415550.1"
                     /translation="MKFDLIISNIMIGILVICSAFALMFVFYIQRKLTPEQYFSFSEF
                     FLIGRGLNIKKILNRMILIVSFNCILFLILKNFFKIELVCSMCILSTLLGSILIIYPA
                     FKIRHDLPNNEMKSLLNYMYVSFVFLALIISTLTVFTLVVLFDDISIVTLWQEHKSGI
                     ILYLLLVIPSLFFNPTNYEKKMEYAIEKHHESEKEIKETLIKNNNETIIPDESQSSSP
                     FKIKKVFRLLNRIMKDD"
     gene            complement(1106..4234)
                     /locus_tag="NBK22_002394"
     CDS             complement(1106..4234)
                     /locus_tag="NBK22_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD2415551.1"
                     /translation="MSFQFSEDDLEQVALKWFEGLGYSVKNGRDSSDNGIMNERESDK
                     DVVFDDRLEAALRRINPELNNSAIEQAMREISIEKSPSLLENNRSFHEMITNGIEVEH
                     YNDDGETINDLVYVFDFDNPGNNDFLAVNQLTVVNGDYKKRPDIVLFINGLPVVVIEL
                     KNSTNESVGIEDGYHQIETYKMRTPQLFNHNAVLVTSDGINTKAGSLTAEYDRFMTWR
                     TKDGKTEDSSTFRSLDILIHGMLNKEVLLDLIRHFVLFQDDGKGNIVKILAAYHQYYA
                     VNKAVDRAMEATSEHGDGKGGVIWHTQGSGKSLTMVFFSGKLIQKLNNPTLVVVTDRN
                     DLDNQLYGTFVKSKGRSGKGLLRQTPKQAESRKELKELLSVESGGIVFTTMQKFEPEE
                     GQVTMDALTERKNVVVMADEAHRTQYGFKATYNEKGEGIKYGYAKYLRDALPNASFVG
                     FTGTPVSSTDKNTQMVFGNYIDVYDMTQSVEDGSTVKIYYESRIIPLNLPTDLDIDDA
                     YDVITSGQEEDTKSRLKSKWSRIEALSGAEKRVHALATDIINHFETRQKAMKGKGMIV
                     TMSRRIAVDLYDEIIRLKPEWHSDDDNKGVIKVVMTGSSSDPQNFQKHIGPKKRRNLL
                     ENRMKDVNDELQLVIVRDMWLTGFDVPSMHTMYIDKPMKGHNLMQAIARVNRVFEDKP
                     GGLIVDYVGIAESLKEALKEYTDSDREQTGIDTEKALELMLMKYDIIQDMLYNHDYSD
                     FESEDQLNRYNAISNTMDYIIGLGEKERERFVTTVTELSKAFALCATEEAAQELNSEI
                     AFLKAVKSGLVKLLAPPSEGSKRKKTPAEIEAEINQLISKSVVTEEVVDIYQTLGIEN
                     PDISILSDDFLKDVEGLQQKNVAVELLNKLLKGQVKSLMKTNTTVSKRFSEMLSKSIQ
                     KYNNRSIEASKVIEELIQMAKEINQEKERGKELGLSTEEIAFYDALASHETAKEAMDD
                     KELRAIAHELTKTVKNNMSVDWSKRESAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVV
                     EQAELMASQQYK"
     gene            complement(4218..>4475)
                     /locus_tag="NBK22_002395"
     CDS             complement(4218..>4475)
                     /locus_tag="NBK22_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD2415552.1"
                     /translation="SLNRGSTQPLLSQKDIKNTKVVMPTNATLLSKYQKKNNHILKMM
                     DQKIIENKKLTQLRDTLLPKLMSGEIEIPDDIEVNEDELSI"
BASE COUNT         1387 a          847 c          548 g         1693 t
ORIGIN      
        1 caatcccaat aatttatcta atgactctaa atataaatta ttagacacgc aaaaactctt
       61 ttttacttga aatggaccta tagaaaagtc tttatttata caatattttt gaaaatactt
      121 tactaatata tactccgcta tctttataat atttgaacct ctatgcatgt tctcaataca
      181 ataaatataa cttaacatat cgaagttatc cttaaaatta acttttaaat gtaaaatttt
      241 gcatttatta ttttcatata aatgtataat tttcttcaaa taatattcaa tcatctttca
      301 taatcctatt caacaatcta aaaacttttt taattttgaa tggtgaagaa ctttgcgact
      361 cgtctggtat aattgtttca ttattatttt ttattagcgt ttcctttatc tctttttcac
      421 tctcatgatg tttttcaatg gcatattcca ttttcttttc ataatttgta ggattaaaaa
      481 atagactagg tatcactaat agaagatata aaattattcc acttttatgc tcttgccaaa
      541 gtgttactat actaatatca tcaaataata ctactaaagt aaatacagta agagtagaaa
      601 taataagtgc taaaaaaaca aaagatacat acatatagtt caacaaactt ttcatctcat
      661 tgttaggtaa atcatgccta attttaaacg ccggataaat aattaaaatc gaacccaaca
      721 atgtacttag tatacacata ctacatacta attcaatttt aaaaaaattc ttaagtatta
      781 aaaacaaaat acagttgaaa ctaacaatca atatcattct attcaaaatt ttcttgatat
      841 ttaaacctct tcctattaaa aagaattctg aaaaagaaaa gtattgttcc ggcgttaact
      901 ttctttgaat ataaaagaca aacattaagg caaatgcaga acaaataact aatatcccta
      961 tcataatatt acttataatt aagtcgaatt tcatatcaat ctccttttct ctttagtcta
     1021 ccggtatatt gacacttttt tatatactct gtcttaaatg tttaatttaa aaaagtatct
     1081 aacaggtcaa caatataaac agtatttatt tatattgttg acttgccatt aattctgctt
     1141 gttccactac ttgttctact gccatttttt gtaagtctgg tggataacca tatttcttta
     1201 atagtcgtct tacttggact ctcattttag cctttgcgct ctcacgtttt gaccagtcta
     1261 cgctcatatt attcttaaca gtttttgtta attcatgtgc aatagccctt aattctttat
     1321 catccatcgc ttcttttgct gtttcatgtg aagctaatgc atcataaaat gcaatttctt
     1381 cagtacttaa acctaattct tttcctcttt ctttttcttg atttatttct ttcgccattt
     1441 gaattaattc ttctatgact tttgatgctt caatagaacg gttattatat ttttgtattg
     1501 atttacttag catctcagaa aaccttttag atacagtagt attagttttc attaatgact
     1561 taacttgtcc tttgagtaat ttgttcaaca attcaactgc aacatttttt tgctgtagtc
     1621 cttctacatc ttttaagaaa tcatctgaca atattgagat atctggattt tcaattccga
     1681 gtgtttgata aatatctaca acttcttcag ttacaactga ctttgaaatc aattgattta
     1741 tttctgcttc tatttcagca ggtgtctttt tacgcttgct tccttcacta ggtggagcta
     1801 ataatttaac aagccctgat tttactgctt ttaaaaacgc aatttcacta ttcagttctt
     1861 gagcagcttc ctctgttgca cataatgcaa atgccttaga aagctctgta actgttgtca
     1921 caaatcgctc tctttctttc tcacctaatc caataatata atccatcgta tttgagatag
     1981 cattatagcg atttaattgg tcttctgact caaagtcaga atagtcatgg ttatatagca
     2041 tatcttgaat gatatcgtat ttcattaaca ttagttctag cgctttttct gtatctatac
     2101 ctgtttgttc tctatcagag tccgtatact cttttagtgc ttccttcaga ctttcagcaa
     2161 tacctacata atcaacaatt aagccgcccg gtttatcttc aaatactcta ttaacacgtg
     2221 caatagcttg cattaaattg tgacctttca tcggtttatc tatgtacatt gtatgcattg
     2281 aaggtacatc aaatcctgtt aaccacatgt ctcgcacaat tacgagttgc agttcatcat
     2341 taacatcttt catacgattc tctagaagat ttcttcgctt tttaggacct atatgttttt
     2401 ggaagttttg cggatcacta gatgaacctg tcataacgac tttgataaca cctttgttat
     2461 catcatctga atgccattcg ggtttaagtc ttataatttc atcatataaa tcaactgcaa
     2521 tacgacgact catcgtgaca atcatacctt tacccttcat tgccttctgt cttgtttcaa
     2581 agtgattaat aatatcagtc gctaaagcat gtactcgttt ttctgcacca gatagagctt
     2641 ctatacgtga ccatttagat ttcaatcgag atttagtatc ttcttcttgg ccagaagtaa
     2701 tgacatcata tgcatcatca atatctaaat cagttggtaa atttaatgga ataatacgac
     2761 tttcatagta tattttaacc gtactaccgt cttctacaga ttgtgtcata tcatatacat
     2821 caatataatt accaaatacc atttgcgtat tcttatctgt cgaagataca ggtgttccag
     2881 taaaaccaac gaacgaagcg ttaggtaaag cgtctcttaa atattttgca taaccatatt
     2941 ttataccttc acctttttca ttgtaagttg ctttaaaacc atattgtgtg cggtgtgctt
     3001 cgtctgccat aactactaca tttttacgtt ctgttaaagc atccattgtc acttgacctt
     3061 cttcaggttc gaatttctgc atagttgtaa atacaatacc acctgattct acagataata
     3121 gttctttcaa ttcttttcgt gattctgctt gtttaggtgt ttgtcttagt aaccctttac
     3181 ctgagcgtcc tttggactta acaaaagtac catataattg attgtctaag tcattacggt
     3241 ctgttacgac cactaatgtt ggattattta atttttgaat taactttcct gagaagaaga
     3301 ccattgtcaa actcttacca gaaccttgtg tgtgccaaat aacgccgccc ttaccatcac
     3361 catgttcaga tgttgcttcc atagcacgat caacagcttt attaaccgca taatattgat
     3421 gatatgcagc taatatttta actatatttc ctttaccatc atcttggaac aaaacaaaat
     3481 gacgaattaa atctaatagt acctctttat ttaacatacc gtgaatcaat atatctaaac
     3541 tacgaaatgt tgatgaatct tcagtttttc catctttagt acgccaagtc ataaaacgat
     3601 catactcagc agttaaagaa ccagcttttg tatttatacc atcactcgta actaatactg
     3661 cattatgatt aaataattgt ggagttctca ttttataagt ttctatctga tgatacccgt
     3721 cttcaatacc aacagattca tttgtagaat ttttcagttc aattaccact actggtaatc
     3781 catttatgaa taacacaata tcaggtcttt ttttataatc tccatttaca acagttaatt
     3841 gattaaccgc taagaaatca ttatttcctg ggttatcaaa gtcaaacaca tagaccaaat
     3901 cattaatcgt ctcaccatca tcattatagt gttcaacctc aataccatta gtaatcattt
     3961 catggaaaga acgattattc tctaataaac taggagactt ctcaatagaa atttcacgca
     4021 tagcctgttc aattgcacta ttatttagtt caggattaat acgtctcaaa gctgcttcca
     4081 aacgatcatc aaacacaaca tctttgtcgc tctcccgttc attcataatc ccattgtcac
     4141 tactatctcg accattctta acagaatagc caagaccctc aaaccacttc agtgcaacct
     4201 gttctaaatc atcttcacta aattgaaagc tcatcttcat tcacctcgat gtcatcaggt
     4261 atctcaattt cacctgacat taattttggt agtaatgtgt cacggagttg tgttaatttc
     4321 ttgttttcta tgattttttg atccatcatt tttaaaatat gattgttttt cttttgatat
     4381 tttgaaagca aagttgcatt ggtgggcatt actactttag tattcttaat atctttttga
     4441 gataatagag gttgtgtaga acctctattc aatga
//
LOCUS       DAHHYJ010000170         4469 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000170 DAHHYJ010000000
VERSION     DAHHYJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.172"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            203..1378
                     /gene="rpsA"
                     /locus_tag="NBK22_002396"
     CDS             203..1378
                     /gene="rpsA"
                     /locus_tag="NBK22_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCD2415553.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLESEEEDNPTIGDMIGDKLKNLKL"
     gene            1600..2910
                     /gene="der"
                     /locus_tag="NBK22_002397"
     CDS             1600..2910
                     /gene="der"
                     /locus_tag="NBK22_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCD2415554.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            2927..3925
                     /locus_tag="NBK22_002398"
     CDS             2927..3925
                     /locus_tag="NBK22_002398"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCD2415555.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLNVNIIATSDMTKAIQFADIYLMALPTKAMREVATQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            4096..4368
                     /locus_tag="NBK22_002399"
     CDS             4096..4368
                     /locus_tag="NBK22_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCD2415556.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
BASE COUNT         1604 a          599 c          897 g         1369 t
ORIGIN      
        1 tatgtataaa catagcaaac tgtattactt tgatacaaaa atggttgtaa taaatattta
       61 tcgatatgac gacttgaata tgataaagtg acatatttat gtatatgact atttcgcaaa
      121 atgtaatcga ggtagaattt cttgacaatt ctgtcagttt ataagatgtt ataaatatgt
      181 agtgtataag gaggcaaaca agatgactga agaattcaat gaatcaatga ttaacgatat
      241 taaagaaggt gacaaagtca ctggcgaggt acaacaagtt gaagacaagc aagttgttgt
      301 tcatatcaac ggtggtaaat ttaatgggat tattcctatt agtcaactat ctacgcatca
      361 tattgatagc ccaagtgaag ttgtaaaaga gggcgacgaa gttgaagcat atgtcactaa
      421 agttgagttt gatgaagaaa atgaaactgg agcttacatc ttatctagaa gacaacttga
      481 aactgagaag tcttatagtt atttacaaga aaaattagat aataatgaaa tcatcgaagc
      541 gaaagtaaca gaagttgtta aaggtggttt ggttgttgat gtaggacaaa gaggttttgt
      601 tccggcttca ctaatttcaa cagacttcat tgaggatttc tctgtgtttg atggacaaac
      661 aattcgtatt aaagttgaag aattggatcc tgaaaataat agagtcattt taagccgtaa
      721 agcagttgaa caagaagaaa acgatgctaa aaaagatcaa ttattacaat ctttaaatga
      781 aggcgatgtt attgatggta aagtagcgcg tttaactcaa tttggtgcat ttatagacat
      841 tggcggtgtt gatggtttag tgcatgtatc tgaactttct cacgaacatg ttcaaacacc
      901 agaagaagta gtttcaattg gtcaagatgt taaagttaaa attaaatcta ttgatagaga
      961 tacagaacgt atttcattat caatcaaaga tacgttacca acacctttcg aaaatattaa
     1021 aggtcaattc cacgaaaatg atgtcattga aggtgtcgta gtaagattgg caaactttgg
     1081 tgcatttgtt gaaattgcac caggtgtaca aggacttgta catatttctg aaattgcaca
     1141 caaacacatt ggtacgccag gtgaagtgtt agaacctggt caacaagtaa atgttaaaat
     1201 attaggtatt gatgaagaga atgaaagagt atcactatct attaaagcaa cattaccaaa
     1261 cgaagatgtt gttgaaagtg atccttctac gactaaggcg tacttagaaa gcgaagaaga
     1321 agataatcca acaattggcg atatgattgg tgataaactt aaaaatctta aactataatt
     1381 taatatttaa tagtcaactc cacatgttta tgattgcatg tggagtattt ttatgtaaca
     1441 aaatatactc agaatgataa cgtggacaaa tttaactaag tgtttaaaaa gataaagttt
     1501 taagtgctga tttttatcat tacagtaata aactcatttt gaatacacag tctcatgtga
     1561 tattattaaa aagatataag aaagagagga agttagctta tgactaaacc tatagtagct
     1621 attgtaggta ggcctaatgt aggtaaatct acaattttta atagaatagt tggagaacgt
     1681 gtttcgattg tggaagacac gccaggtgta acacgagatc gtatttattc ttcaggtgaa
     1741 tggttaacac atgatttcaa tattattgat acaggtggta ttgaaattgg tgatgcacca
     1801 ttccaaacac aaattagagc gcaggcagaa atcgccatag atgaagcgga tgttattatt
     1861 tttatggtta acgtgcgtga aggattgaca caaagcgatg aaatggtcgc tcaaatttta
     1921 tacaaatcta aaaaaccggt cgtattagcg gttaacaaag tagataatat ggaaatgcgt
     1981 acagacgtgt atgatttcta ttcattagga tttggtgaac cgtatccgat atcagggtca
     2041 catggtttag gtcttggtga cttgttagat gcagttgttt ctcattttgg tgaagaggaa
     2101 gaagatcctt atgatgaaga tacaattcga ctatccatta ttggacgacc aaacgtaggt
     2161 aaatcaagtt tagtaaatgc tattttaggt gaagatcgcg ttatcgtttc taatgttgca
     2221 gggacaacga gagacgctat tgatacagag tatagttatg atggacaaga ttatgtttta
     2281 atcgatactg ctggtatgcg taaaaaagga aaagtatatg aatcaactga gaaatattca
     2341 gtattaagag ctttaaaagc gattgaacgt tcaaatgttg ttttagtggt catagatgca
     2401 gaacaaggca tcattgaaca agataaacgt gttgcaggat atgcacatga acaaggtaaa
     2461 gcagtcgtga ttgtcgtaaa taaatgggac actgtggaaa aagatagtaa aacgatgaag
     2521 aaatttgaag atgaagtacg taaagaattc caatttttag attatgcaca aattgctttt
     2581 gtgtctgcta aagaacgcac aagattacgt acattattcc cttacatcaa tgaagcaagt
     2641 gaaaaccata aaaaacgtgt tcaaagttca actttaaatg aagttgttac tgatgcaatt
     2701 tccatgaacc ctacaccaac agacaaaggt agacgtttga atgtctttta tgcaacacaa
     2761 gttgctatag aaccaccgac atttgttgta tttgttaatg atgtagaatt aatgcatttt
     2821 tcttataaac gctatttaga gaatcaaatc cgtgccgctt ttggttttga aggtacacca
     2881 attcatatta tagctcgaaa gagaaattaa cgattggggg ataacaatga ctaaaattac
     2941 cgtttttggt atgggaagtt ttgggacagc ccttgccaat gttcttgcag aaaatggaca
     3001 tgatgttttg atgtggggta aaaatcaaga tgctgttgat gaattaaata catgtcatac
     3061 aaataaaaag tatttaaaat acgcgaaatt aaatgttaac atcatcgcta cttcagatat
     3121 gaccaaagca attcaatttg cagatattta cttaatggct ttacctacta aagcaatgcg
     3181 agaagttgct actcaaatta atgataagct gacatctaaa aagactttta tacatgttgc
     3241 taaaggtatt gaaaatggga cgtttaaacg tgtgtcagaa atgattgaag attctatttc
     3301 acccgaatat aatgcaggta ttggcgtgtt gtcagggcca agtcatgcgg aagaagttgt
     3361 agtcaagcaa ccaactacag ttgctgcatc atcaaaagat aaaagtgtaa gtaaattaac
     3421 gcaagattta tttatgaatg attatttgcg tgtgtacacg aatgatgact tgattggtgt
     3481 tgaacttggt ggtgcattga aaaatatcat cgcagtagca agtggtatcg tagctggaat
     3541 tggctacggt gataatgcaa aagctgcgtt aatgactcgt ggtttagcgg aaattagtag
     3601 attaggtgaa aagttaggtg ccgatcctat gacatttcta ggtttaggtg gtatcggtga
     3661 cttaatcgtt acttgcacat caacacattc tcgaaatttc acattaggat ataaacttgg
     3721 acaaggtgaa tcaatggatc aagcattatc tgaaatgaat atggttgttg aaggtattta
     3781 tacaactaaa tcagtttatc atttagctaa agaaaaaaat gtggatatgc caattacaaa
     3841 tgcattatat agagtattat ttgaaaatat ctcagtaaaa gaatgcgtaa aagatttaat
     3901 ggagcgcgat aaaaaatctg aataaaatgt gtattattac acatatacgg ctaataatta
     3961 cgtaaaatga atagaaatgg cttaaattca acgtttttgc cacataaatg attgcgattt
     4021 atgtaatgct tgtgttaaag ttttaccata atgatataaa catatcatta tcaaacctca
     4081 ggaggtgaat gtctaatgaa caaaacagat ttaatcaatg cagttgcaga gcaagctgat
     4141 ttaactaaaa aagaagctgg ttcagcagta gatgctgtat tcgaatcaat ccaaaactca
     4201 cttgctaaag gtgaaaaagt acaattaatt ggtttcggta actttgaggt acgtgaacgt
     4261 gctgcacgta aaggtcgtaa ccctcaaact ggtaaagaaa ttgatatccc agcaagtaaa
     4321 gttccagcat tcaaagctgg taaagcatta aaagatgctg taaaataatt ttacttaaaa
     4381 agccctgaat aagggctttt tattttgctt ttaatactta caacctgtac ataatttgta
     4441 atgttcttct aagtttttaa tctttggaa
//
LOCUS       DAHHYJ010000171         4450 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000171 DAHHYJ010000000
VERSION     DAHHYJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.173"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(29..184)
                     /locus_tag="NBK22_002400"
     CDS             complement(29..184)
                     /locus_tag="NBK22_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415557.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIIYSKLIVRKFKIF
                     VDILKQI"
     gene            222..683
                     /gene="tsaE"
                     /locus_tag="NBK22_002401"
     CDS             222..683
                     /gene="tsaE"
                     /locus_tag="NBK22_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCD2415558.1"
                     /translation="MIKINNLDEMNQFAIFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPATHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            664..1326
                     /gene="tsaB"
                     /locus_tag="NBK22_002402"
     CDS             664..1326
                     /gene="tsaB"
                     /locus_tag="NBK22_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCD2415559.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGEVIAQLPNASVMYQLLDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            1299..1763
                     /gene="rimI"
                     /locus_tag="NBK22_002403"
     CDS             1299..1763
                     /gene="rimI"
                     /locus_tag="NBK22_002403"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCD2415560.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDLERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            1756..2781
                     /gene="tsaD"
                     /locus_tag="NBK22_002404"
     CDS             1756..2781
                     /gene="tsaD"
                     /locus_tag="NBK22_002404"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCD2415561.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2883..>4450)
                     /locus_tag="NBK22_002405"
     CDS             complement(2883..>4450)
                     /locus_tag="NBK22_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCD2415562.1"
                     /translation="LLTSVIGIVGRYMSRQRLLKSMETLWQTISPLEAFIRPNSHFDY
                     EYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMKLYACLRGMLSITNKS
                     LLSLFNDNAEFRKNVTYHLALIGKTVYPTFPDQITPVKRQNILFLCPFLPVISFAVIF
                     INSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLKQGYTISKIKHAPQPE
                     VNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLFHSIQKSYTTHMNELK
                     NCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELTHPLIADAVANDFSLS
                     QNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPGIVFTSMANADDVLSG
                     DSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAASESVLSFLHEKSNFRV
                     IAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKANTRNAIELLKITSFPA
                     KIYERAKDNVPKI"
BASE COUNT         1526 a          627 c          824 g         1473 t
ORIGIN      
        1 tgtctaaaaa tttgataaat ttaatttact atatctgctt tagtatgtca acaaatattt
       61 taaattttct tacaattaac tttgaataga taatctgttt caaagataag aaatttaagt
      121 tgaaatttgt tacaatataa aggttaaata taaagttaga tacgattgaa caatggataa
      181 tcatttttat gaatgaaaag cataatatag gagaatcaac attgataaag ataaataatt
      241 tagatgaaat gaatcaattt gctatatttt tagttgagca attgaaaagt ggtgatttga
      301 ttttacttaa cggagattta ggagcaggta aaacaacgtt aacgcaattt ataggaaaag
      361 ctcttggtgt aagacgtacg attaattccc cgacatttaa catcattaaa tcatataggg
      421 gtaaaaattt aaaattgcat catatggatt gttatcgctt agaagattct gatgaagatt
      481 taggatttga tgaatttttc gaagatcagg caattactgt tattgaatgg agtcaattta
      541 taaaagattt acttccagcg acgcatttat ctattaacat ttcaacaata tctgaaaata
      601 caagacaaat tgagttgttc gcgcaaggag aacattatga acaaattaag gaggcaatta
      661 tccatgaatt cgctgctcat tgatacatcg aatcaaccat tatcagtagc attgatgcaa
      721 aacgatgaag ttttagctga aataactacg gattctaaac aaaatcattc agtgcaatta
      781 atgcctgcga ttagtcagct atttgagcag agcaaaatag ctaaacagca attagatgcg
      841 attattgttg ctgaaggtcc tggttcatat actggcttac gtatcggtgt tactgttgct
      901 aaaacattag cgtatgcgtt agatgttaaa ttatatggtg tttcatcatt aaaggcattg
      961 gcagcaacaa tcgatcatac tgataaatta ttggttcctg tgtttgatgc aagacgacaa
     1021 gcagtttata caggcatatt ccagtggcaa aatggtcaat tagtgactat tttagaagat
     1081 caatatatgt cgatagaggc attgcgagct tttttagaag atttaaacca accatttatt
     1141 tatattggga aagatactgt aaaattacaa gatgaattac aaggtgaagt gattgcgcag
     1201 ctaccaaatg catcagtcat gtatcaattg ctagataaac cttcagacgt acatacattt
     1261 actcctaaat accataaatt agctgaggcg gaacgaaatt ggatcaacag tcaaaagaac
     1321 aattaaacat tagagagatg actaaagaag atgtgccaca agtctttgat cttgagcgtc
     1381 ggagtttcaa tgacagttct tggacaattg acgcatttta tcatgaaata gagcaaaata
     1441 actttgcgaa atattttgtc ttagaatttg aacaacaaat tattggttat ttaggtttat
     1501 ggattgttat cgatcaagct caaattacaa cagttgcaat cgatgataat tatagaggct
     1561 atggtttagg tcaaatgctt ttaaaatacg gaataaacta tgcaagtcac acttgtgatg
     1621 tgatgagttt agaagtaaga gttaataata aagtggcaca acatgtatat gaaaatttag
     1681 gatttcaata tggtggtaaa cgtaaaaatt attatggtga aggtgaggac gcaatggtta
     1741 tgtgggtgaa tttaaatgac taaagatata ttaatactag ctgttgaaac aagttgtgat
     1801 gaaacaagcg ttagtgttat aaaaaatggc agagatattt tatcaaatac agttttaagt
     1861 cagattgaaa gtcataaacg atttggcggt gtcgttcccg aagtggcaag tagacatcac
     1921 gttgaaggta taacagcaac aataaacgag gctctagggg atgccgatgt atcaatagaa
     1981 gatattgatg ccatagcggt tacagaaggc cctggactaa ttggtgcgtt actaataggt
     2041 gttaatgcag ccaaagcatt ggcatttgct tacgataagc cacttattcc tgttcatcat
     2101 attgcaggac atatatatgc taatcacata gaagagccat taacattccc gctaattgca
     2161 cttattgttt caggtggaca tactgaatta gtttatatga aggatcattt atcatttgaa
     2221 gtcattggtg aaacacgaga tgacgcagta ggtgaggctt atgataaagt ggcacgaaca
     2281 attggtttaa attatccagg tggtccacaa gttgatcggt tggctgctga aggtgaagat
     2341 acttattcat tccctcgtgt ttggttggat aaagatagtt atgattttag ttttagtggg
     2401 ttgaaaagtg ccgtaatcaa tcaacttcac aatcaacgac aaaaaaatat tccaatcatt
     2461 gaagctaacg tagcaacgag ctttcaaaac agtgttgtag aggtgctcac gtttaaagct
     2521 attcaagctt gtaaagaata tggtgttcag cgattaattg ttgctggtgg cgtggcgagt
     2581 aataaaggat tacgtcaatc tttagcggat caatgcaaag tcaatgacat tcaattaact
     2641 atcccaagtc ctaaattatg cacagataat gctgcaatga taggcgttgc cggccactat
     2701 ttgtatcagc aaggtcgatt tgctgattta gcattaaatg ggcacagcaa tatagattta
     2761 gaagagtatt ctgcagaata aaataaacgt cagttattaa catgattact atgaacatta
     2821 ttgaaactgt tcatagtctt ctgtcatgtg atagctgacg tttttatgtt taaagttaaa
     2881 tgctaaattt tcgggacatt atcttttgct cgttcatata tttttgctgg aaatgaagtg
     2941 atttttaata attcgatggc attacgtgta tttgctttgc caggcttaat tttgtaatca
     3001 aaatgtatgt tattattttc tattacctca ttgaaatggt aattttcata acgttgtttt
     3061 aagagttcag ctaactcaat atcatgtgtt gctgcaataa ctctaaagtt agatttttca
     3121 tgtaaaaatg atagtactga ttctgaagcg gcaattcgtt cagttgtgtt ggtaccttta
     3181 aaaatttcat ctataaagca gtatattttt tgattatctg gaatttcaac aatacgctta
     3241 atagatttta attcagccat aaaataacta tcgcctgata acacatcatc ggcatttgcc
     3301 attgacgtga acacaattcc aggttgatat acaaatttgc ttgcggtaac tgtttgtata
     3361 gccgatgcaa gaataatgtt tattgctatg gatttcataa atgttgattt acccgaagca
     3421 ttagacccag ttaacagtat attttgtgat agtgagaaat cattggctac tgcatctgct
     3481 atgagtggat gtgttaactc agagaatact atgccatcat ttgaatcatc aatttgtggt
     3541 tcagtataac attctagcgt tctacgatac attgctaatg cataatgatt atctaattca
     3601 gctatgtaat cgaaacaatt tttaagctca ttcatatgtg ttgtatagct tttttgaatt
     3661 gaatgaaata aaacataatc aagcatgaaa ataagtttaa ctagtttaat taccatagct
     3721 ccgatatctt catcattaac ttcagctaaa acactcgtta aatgacgtgc tgttctaaat
     3781 tgcttaaaat tgacttcagg ttgtggagca tgtttaattt ttgagatagt atagccttgt
     3841 tttaaaacat tggatgcata aaagatagat tttaagtcat cctcatatgt acgctttaaa
     3901 gttgctgata aaatgatatt aaataggcaa ctcataagaa ataataaaat accaacttgt
     3961 gaattaataa atataactgc aaatgagata actggtaaaa acgggcatag aaacaatata
     4021 ttttgacgtt ttaccggtgt gatttggtct ggaaatgttg gataaacagt tttaccaatc
     4081 aaagctaaat gatatgttac gttttttcta aattcagcat tatcattaaa taaactaagt
     4141 aatgatttgt tcgtaattga aagcattcca cgtaaacagg catatagctt catttcacca
     4201 atagctgtta aattataatt catcttatga aagattgcat tcatatttaa gtcggaccaa
     4261 gttttatcat caactaatga atgtgattca aatttttcct tgtagagctt atactcatag
     4321 tcgaaatgtg agttcggtct gataaaagct tctaatggag aaatcgtttg ccataatgtt
     4381 tccatagatt ttaatagacg ttgacgactc atgtatcgtc caacaattcc tattacagat
     4441 gtaagtagaa
//
LOCUS       DAHHYJ010000172         4407 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000172 DAHHYJ010000000
VERSION     DAHHYJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.174"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(9..182)
                     /locus_tag="NBK22_002406"
     CDS             complement(9..182)
                     /locus_tag="NBK22_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415563.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
     gene            633..1673
                     /locus_tag="NBK22_002407"
     CDS             633..1673
                     /locus_tag="NBK22_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCD2415564.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYHPETLTAYFTLGENAPPEIIDFKSWLDGQDLNITHFT
                     GNIDTDLTFANK"
     gene            complement(1730..2572)
                     /locus_tag="NBK22_002408"
     CDS             complement(1730..2572)
                     /locus_tag="NBK22_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCD2415565.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNLSSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIENSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(2569..3126)
                     /locus_tag="NBK22_002409"
     CDS             complement(2569..3126)
                     /locus_tag="NBK22_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCD2415566.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEG
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            complement(3186..3710)
                     /locus_tag="NBK22_002410"
     CDS             complement(3186..3710)
                     /locus_tag="NBK22_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415567.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            3831..4394
                     /locus_tag="NBK22_002411"
     CDS             3831..4394
                     /locus_tag="NBK22_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD2415568.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRADKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
BASE COUNT         1589 a          751 c          565 g         1502 t
ORIGIN      
        1 aaatgttatt agttattttt actttcaata tcagaaataa tttctttagt ttttctttca
       61 atatcttcaa aatctttttt aaagatataa gcaaatgcta ctacaccaac accagcaact
      121 aatgttgtaa tagcaactaa gctaaatacg aatttaaaca cacatttaat aaaattccac
      181 atctcaattt cacctctgtt aaattaaata cctatctaca tttcattata ctatcatata
      241 tagaataatc attaaataaa tacccacttt tacgaaatat attcatcaaa ttatatagcg
      301 agcaagctaa tgattatttt caaaaatgcc gaggaacctc tgtttcaacg tgacatgacc
      361 ttatatgcta atgttaacaa tgactattat cgtgaatgct aacttaagat tgatatttat
      421 gataagatga ttttaagttt ttaactcaat atccccacca tctataaaaa tatgttttaa
      481 ctgattgata agtcttatac accgagaaat cacctcaaac aattttatat ttcatttagt
      541 aaaatataac tttatttaac atgacattca atatacatac gattactatt tatttacaat
      601 atattacaac taaaaagaaa ggggccgatg atatgcaaca agtgacatca gatattatga
      661 cttttagagg ttcacatttc gagttaggtg ttaaaactgg taaatggctt caacaaacac
      721 ctcttctaaa aaatcgagaa aaagaatgga aaaaacgtgt cccacgtttc gatatagatg
      781 taaatgaaac ttatcaaata ttccaaacat acgcacctca aatatgggaa gaattaatgg
      841 gactacaaag tatactcaaa atgccaacac gccaaattat tttaaacttt ggacattatc
      901 gttttaccga tttaaaagaa agtggttgca cggtctttca aggcaaagac ttcatggtta
      961 gaaattatga ttaccaccct gcaacttatg atggtcgcta cttactttat caacctacag
     1021 atagcggttt agctcaaatt ggtcctgtat caagggtaac tggaagaatg gatggtatga
     1081 atgagtcagg attaactatg ggctataact ttatgcaccg gaagaaacca gcaaatggtt
     1141 ttgtatgtta tatgattggt cgtctaattc ttgaaaattg ccgaaatgta acagaagcaa
     1201 tccaattatt gaaagaaata ccacacagaa gctcatttag ctacatactg atggacaaat
     1261 cattgaacca tgcaattgtt gaagtgacgc cgagatcaat agatgttcgt tatgataata
     1321 tatgtacaaa tcattttgaa atattaacac atgaaaatcg caattataca aaagaatcta
     1381 aagaaagact ggcgcgtacc atttcacaaa ctaatgataa tttagatatg actacagcat
     1441 ttaagttatt caataatcct cagtatgaaa tttacagtaa actattcaaa agctggtctg
     1501 gcaccatcca tacatcaatg tatcaccctg aaacattaac tgcatatttt acattgggag
     1561 aaaatgcgcc accagaaata atagatttca aatcatggtt agatggtcaa gaccttaata
     1621 tcactcactt tactggcaat atagataccg acttaacttt tgccaacaaa taaataacac
     1681 cgatattaga gaaattatgc ttcctaatat cggtgcttac atcctttatt tattcaactt
     1741 tgatattacc atcaatcgta taaaattcta aaacattgtc gcttttccca actttaccat
     1801 tagtaaatgt tttattttta actacgctat cacccgttcc cggatttagc tttagtatag
     1861 tgtcttcagg tttactatca tacttaaaac gaatatctcc ttgtttagta gaagcttttg
     1921 catctacttt tgatggcatg tttttaaatt caatgtcacc atgatcattt aaaaatatgg
     1981 attgtttaat ttgactcttg tcgatttgta gcgtaccaat aacaacttta atattgctat
     2041 tttcaatgtt acttttacta atataagtag aactattatt tgtctttgaa tctagtgcgt
     2101 caagatttga gtttttaata actacttcac cgttaatgct ttgaatactt gtgttctcta
     2161 aatcaacatc actgattcta acacttcctg ctccagatga tagatttaaa cgtttaatca
     2221 ttttatcagg catttcaatc gttaacgttt tcttattctc atgaaaagga ttcatatcaa
     2281 ttgcatatcc tcttgtctta gaccttttat cactaatctt caacgtctta tctactatat
     2341 taattaatat atcattgtca ccatcatatt taactctaaa ttgattcccc cgttttatac
     2401 gaagttctgt actatccaaa tttatagata cattgtcaaa agcatcgtct ttgaatgttt
     2461 tatcatattg tttagtacca tatttgtttt tatcatagct gaaccaaata atggttgctg
     2521 ctgtaaaaaa gacaacaaac actaaaagcc caataaaaaa gaatttcttc attttctaac
     2581 gcttcctttg acagttttaa tataccaaag taaatatttt aagattaaac gataaatgta
     2641 ttctaaaatc ttaaatatga caatgataaa tactaatcct aatcctgaat atgatatggc
     2701 aaaaaataca ttactaattg agtccacaat tcctctggat attgctgatg ctaataaaat
     2761 aattggacaa aacagcagca aaagtgatat taaaaataat gctaatacaa ataatccaac
     2821 tatagatact ggtataagga tgacaaaaat agatagaatg cctaaactta atgaagcagc
     2881 aacagctctt gaaatatttt caaatgttgg tttagattca gcataatcaa taacacttct
     2941 agcttttgtt tcagctgcaa catcttgtgg gtcttttaac ttctttaaaa tttccccttc
     3001 atcaacgcca ttaagttctt gctcataaaa ataacgttca tattcataca ttttttgatc
     3061 tcttaaatgc ctaggtagcg catcaagtgc tgtttctaat tcatttaaat aagtaatctt
     3121 gttcatatag gtcactcatc ccaataaaat tattcattcg ttttttataa ctttaagacc
     3181 taaagctatt tactaaaaaa ttcaatacgt ttaatttcta aatcttgata aagtaagttt
     3241 aattttaaat ggttatctct atcaatatac tttaccgatg agaaatcatt gccaactttt
     3301 gttcttttat atccgaatcc aagattttca atcgcgttat ttatcggcga accaattccc
     3361 ccatcaaaat tagtatgtat cttttcatct cttactaata ttatcccctt aacgatgtta
     3421 tccttttctc tcactttaac ggtaagatca gggtgagctt tactgttata aaatttaaaa
     3481 cgatcaaact taattttttt atttacttca tagccttttt tatcaaactt ctcatttaat
     3541 ttaatatcgt ttatttcaag attagacaaa tccacagttt tcgtatgacg atattgatat
     3601 aaaccaatta atacaccaaa agaacagatt aaaaataata taaacacaac ccagcgattt
     3661 tgaatacgca ctgatgtcac cctttctttg tacttattta aatatagcat aaggtgcttc
     3721 ccgtttcatc cttaacaaaa attaacaaag aatttgcaat taatttattt cattaccttt
     3781 tattactata aattggtata atgttgtaaa tgctaaagga ggtttttaca atgacaaatt
     3841 tagaaactta tttcaaaaat agccaaccct taaatgaata cattgatgga atgaagacaa
     3901 atcaagatac cgttctcagt atctatcaat catttgagtt accagaagat gattcacgca
     3961 tcaaaaaaat aaaagagatg aattattcta aagtattagt aattactgaa gactggtgtg
     4021 gagatgcaat gatgaatctc ccaattctaa aacacatcag tgaagcatta aatttagaag
     4081 tacgcgtgtt ccatagagat gatgatacaa aactgatcga tcaatattta acaaatggta
     4141 aatcacgtgc aattcctatc ttcgtattct tgaatgatca attcgaacaa gaaacagttt
     4201 ggggaccaag agcaaatgaa gtccaaaaat ttgtaactga tgttcgtgct gataaattac
     4261 cgagcaaaga tcatccagac tatgcagatt tagaaaaaga aacacactta gttatatcaa
     4321 accgctataa aactgacgct tcattttgga aagcagttta taattccatc ctaaataaat
     4381 tagaaaataa ataaatttac aataaga
//
LOCUS       DAHHYJ010000173         4180 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000173 DAHHYJ010000000
VERSION     DAHHYJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.175"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            132..782
                     /locus_tag="NBK22_002412"
     CDS             132..782
                     /locus_tag="NBK22_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HCD2415569.1"
                     /translation="MKITYKYRGDLPLNTENNKNQNQSVKNSERRGMLKGCGGCLISF
                     ILLIILLSACSMMFSNNDNSTSNQSSKTQLTQKDENKNEDKPEEKSETATDEDLQSTE
                     EVPANENTENNQHEIDEITTKDQSDDDINTPNVAEDKSQDDLKDDLKEKQQSSNQHQS
                     TQPKTSPSTETNTQQSFANCKQLRQVYPNGVTADHPAYRPHLDRDKDKRACEPDKY"
     gene            863..1858
                     /locus_tag="NBK22_002413"
     CDS             863..1858
                     /locus_tag="NBK22_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCD2415570.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLGPTNAKGIFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QNDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SDAKKTSKAKKDKQDTEVAVASSNSDNVSYEASATTPATTSSANNDSEENEQSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEHMPQSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            1934..2560
                     /locus_tag="NBK22_002414"
     CDS             1934..2560
                     /locus_tag="NBK22_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415571.1"
                     /translation="MKFKAIVAITLSLSLLTACGANQHKENSSKSNDTNKKTQQTDKT
                     TQSNREKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNENEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGKIINIFDDMSEKDKEEFEAFKKRSPKYNPGMSDQAEMDNESEDI
                     QHHDNDNNKAIQNDIPDQKVDDKSDKNAVNKEEKHDNGTNNSEETKVK"
     gene            2601..2942
                     /locus_tag="NBK22_002415"
     CDS             2601..2942
                     /locus_tag="NBK22_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCD2415572.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     MESLSSLGVKPDIIDLIHKGTELEDFAAFNLSIEDTVTVCLQRTEELLNQYKNVEFND
                     KILINWRIIQE"
     gene            3043..3615
                     /locus_tag="NBK22_002416"
     CDS             3043..3615
                     /locus_tag="NBK22_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415573.1"
                     /translation="MEKNEYTAKYNEYSQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSISKGKYTKASEGLYCHHISEDKFQNLSDLRFISKFKYSYDDQKKENL
                     VYCDLIEHLILHAIITKESDGQFGAAGLCQMIKPTVIEWYIGEYDPKPAWMQPTKARA
                     YLPGILVEKLLIKIDDMLEGIEIYDFLESR"
     gene            complement(3813..>4180)
                     /locus_tag="NBK22_002417"
     CDS             complement(3813..>4180)
                     /locus_tag="NBK22_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCD2415574.1"
                     /translation="GYLSGRTIYINNNHSINNTRFAIARELGRYLYKIKDNNDNSLKN
                     LHEMIYESDVNQFSVDLLMPEKQIEALVYNFYEVNNINLNSGLSEKERNKLLNLISTK
                     LEVSKVAAGLRLYNLGIHI"
BASE COUNT         1658 a          651 c          679 g         1192 t
ORIGIN      
        1 tttttaaagt tttgaaaaaa attaaagtat ttccactact aatttggtaa aatatgatta
       61 tgtgatatag tgaaagcgtt ttacacttaa taactccctc ttaaatgcat ccaggtttta
      121 tgtagtaaat catgaaaata acatataaat atagaggaga tttacctttg aatacagaga
      181 acaacaagaa tcaaaaccaa tctgttaaaa attctgaaag acgcggcatg ttaaaaggat
      241 gcggcggttg ccttatttct tttattttat taataatctt attatcagcc tgttcaatga
      301 tgtttagtaa taatgacaat tccactagta atcaatcatc aaaaacgcaa ttaactcaaa
      361 aagatgaaaa taaaaatgaa gataagcctg aggaaaaatc agaaacagca acagatgagg
      421 atttacaatc aaccgaagaa gtacccgcaa atgaaaatac tgaaaataat caacatgaaa
      481 ttgatgaaat aacaacaaaa gatcaatcag acgatgatat taacacacca aacgttgcag
      541 aagataaatc acaagacgac ttgaaagatg atttaaaaga aaagcaacaa tcaagtaacc
      601 agcatcaatc cacgcaacct aagacctcac catcaactga aacaaacacg caacaatcat
      661 ttgctaattg taagcaactt agacaagtat atccgaatgg tgtcactgcc gatcatccag
      721 catatcgacc acatttagat agagataaag ataaacgtgc atgtgaacct gataaatatt
      781 aaacaacaag cgaattgaat tcaaattgta tttagcttta tgcactaatc acatagtaaa
      841 taatgaggga gattttttag gcatgagcaa tcaattcaaa agcgaagaag agcgaagaca
      901 atgggaacaa ttccaagctt tccaaaatca acaaaaccaa cagaaccagc aatacggaca
      961 aaagaaatct aaaaaaggat ggttctgggg ctgtggtggt tgtctagtat tatttatttt
     1021 aattatcatc ggtatttcag cttgtacagc tggtattaca ggtaaccttg gcggaaatag
     1081 ttctaaagaa acgaacaaaa cccataaaat cggtgaaact gttaaaaatg gcgaccttga
     1141 agtcactgta aattcagtgg aaactatgaa atctgtagga ccatctcttg gaccaacaaa
     1201 cgctaaaggt atatttgtcg ttgctgatgt gacgattaaa aacaaaggta aagaagcatt
     1261 aacaattgat agttcaatgt ttaaactgaa atctggtgat aaaacatttg aagcagataa
     1321 tacaggctca atgtctgcta atcaaaatga caacggtagt atagaaaatt catttttctt
     1381 acagcgtata aatcctgata gcactgctca aggtaaaatt gttttcgatg tgtcagaaaa
     1441 catagccaac gcaaaagata aaaaattaga agttatttct agtttattta gcgtcaagaa
     1501 gattacattt gatttatccg atgctaaaaa aacatcaaaa gctaaaaaag acaagcaaga
     1561 tacagaagta gctgttgcga gttcaaatag cgataatgta agttatgaag cttcggctac
     1621 tacacctgct acaacttcta gtgcaaataa tgattctgaa gaaaatgaac agtctagtaa
     1681 agatgaagat aagcagaatg cgtctaaaag tgataaatct agtgtagaaa aaagtgaatc
     1741 taatgaagaa actgctcctg tagagcacat gccccagagc aaacctacca ctagtgaagc
     1801 accacctagc caaaatattc ataatgaaga tagcatgtac gacgcttcaa cagaataaaa
     1861 tttcagggta gctcggctac ccttctttta cggaaaaatt aattatacat aatcaaatca
     1921 aggagataaa aaaatgaaat tcaaagctat cgttgcaatc acattatcat tgtcactatt
     1981 aactgcctgt ggtgctaatc aacataaaga aaatagtagt aaatcaaatg acactaataa
     2041 aaagacgcaa caaactgaca aaactacaca gtcaaataga gaaaagcaaa tgacaccaca
     2101 agaagccgaa gatatcgttc gaaacgatta caaagcaaga ggtgctaacg aaaatcaaac
     2161 tttaaattat aaaacaaatc ttgaacgaag taatgaaaat gaatattatg ttgagcatct
     2221 agtccgcgat gcagttggca cacctttaaa acgttgcgca attgttaatc gacacaatgg
     2281 taagattatt aatatttttg atgatatgtc agaaaaagat aaagaagaat ttgaagcatt
     2341 taaaaagaga agccctaaat acaacccagg tatgagtgat caagctgaaa tggataatga
     2401 gtcagaagac attcaacatc atgacaatga taataacaaa gccattcaaa atgacatacc
     2461 agatcaaaaa gtcgatgata aaagtgataa aaatgctgtt aataaagaag aaaaacacga
     2521 taacggtaca aataattctg aagaaactaa agttaaataa gggtatactt tgattaatcg
     2581 taatttttag gagaaccgct atgataacat ttgaaaatat acaacaactt gaaaaatata
     2641 ccttaatgac tatgcatggt ctttttaatc aacttaaact cggtattatt tcaatcgaca
     2701 atgcagagca taccctcttt acaccttata tgatggaatc actctcttcc ctaggtgtga
     2761 aaccagacat tatcgattta attcacaaag ggacagagtt agaagacttt gcagcattta
     2821 atttatcaat tgaagataca gttacagtct gtttacaaag aactgaagaa ctattaaatc
     2881 aatacaaaaa tgtggaattc aatgacaaaa tattaatcaa ttggcgtatt atacaagagt
     2941 aatagacata taaaagtcga atgtaactac gtgagtactg attttattct ttgtaaatta
     3001 caagcatttc atattataaa gtttgaaaag aggtatattg aagtggagaa aaacgaatat
     3061 acagctaaat ataatgaata tagtcaatta ttagacgcta cttactcgca agctgtagca
     3121 taccttttaa ataaatatgg cgctgtaacc gatgattatt ataaggaaaa atcatacacg
     3181 cgatttttaa atggagaaat caaaagtatt tctaaaggaa aatacactaa agctagcgaa
     3241 ggattatatt gtcatcacat aagcgaggac aaattccaaa atctatctga tctaagattc
     3301 atttccaaat ttaagtactc atacgacgat caaaagaaag aaaacttagt ttactgcgat
     3361 ttaatcgagc atttaatttt acatgcaatt attacaaaag aatctgatgg tcaatttggc
     3421 gcggctggat tatgtcaaat gatcaaacca acagtcattg aatggtacat tggcgaatat
     3481 gatccaaaac cagcttggat gcaacccact aaagcacgtg cctatttgcc tggaatatta
     3541 gtagagaagt tactcattaa aattgacgat atgttagaag gaatagaaat atatgatttc
     3601 cttgaatcgc gataaatgat taatgtagat ttattttttg ctgttcagat tttgttatag
     3661 atgtttaaaa cgataattaa atatatttta taaaaagaga ccacgcatac caatctataa
     3721 acggtcaatg tttataattg agtttgcatg gtcttgaatt gtattagttt tcttttggtt
     3781 ttaaaaaatc gactagattt tcacaatatt tatcaaatat gtattcctaa attatacagc
     3841 cttaatccag cagctacttt cgaaacttcc aacttagttg atataaggtt caatagtttg
     3901 tttcgttctt tttcagataa accagaattt agattgatat tattgacttc ataaaaatta
     3961 tagactaatg cctctatttg cttttcaggc ataagtaagt cgactgaaaa ctgatttacg
     4021 tcgctttcat aaatcatttc atgtaaattc tttagactat tatcgttgtt atctttaatt
     4081 ttgtataaat aacggccgag ttcacgagct attgcaaatc ttgtattatt aatcgagtga
     4141 ttattattaa tatagattgt tcttccactt aaataacccg
//
LOCUS       DAHHYJ010000174         4110 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000174 DAHHYJ010000000
VERSION     DAHHYJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.177"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            37..759
                     /gene="sasD"
                     /locus_tag="NBK22_002418"
     CDS             37..759
                     /gene="sasD"
                     /locus_tag="NBK22_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCD2415575.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMKDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGINHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMQSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTN"
     gene            complement(953..1708)
                     /locus_tag="NBK22_002419"
     CDS             complement(953..1708)
                     /locus_tag="NBK22_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2415576.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYNNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            1944..2651
                     /gene="deoD"
                     /locus_tag="NBK22_002420"
     CDS             1944..2651
                     /gene="deoD"
                     /locus_tag="NBK22_002420"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD2415577.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2658..4010
                     /gene="tet(38)"
                     /locus_tag="NBK22_002421"
     CDS             2658..4010
                     /gene="tet(38)"
                     /locus_tag="NBK22_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCD2415578.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQKKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         1365 a          637 c          732 g         1376 t
ORIGIN      
        1 aaatgtaatc aaattgtaat ataaggggaa aagacaatga aaaaattagc aacagtaggt
       61 tctttaattg taacaagcac tttagtattc tcaagtatgc cttttcaaaa tgcgcatgcc
      121 gacacaactt caatgaatgt gtcgaataaa caaagccaaa atgtacaaaa tcatcgtcct
      181 tatggcggag tagtaccaca aggaatgacg caagcacaat atactgaatt agagaaagct
      241 ttaccccaat taagcgctgg cagtaatatg aaagactata atatgaaatt gtatgatgcg
      301 acgcaaaata ttgctgataa atacaatgtg ataattacaa ctaatgtagg ggtatttaaa
      361 ccacatgctg ttagagatat gaatggccat gcgttacctt taacaaaaga tggcaatttt
      421 tatcaaacga atgtagatgc aaatggtatt aatcatggtg gtagtgaaat ggtgcaaaat
      481 aaaacaggtc atatgagtca acaaggccat atgaatcaga acacacacat gaaccaacag
      541 ccacacatgc aacaaggtca tatgcaatca tcaaaccatc aaatgatgag tccaaaagca
      601 aatatgcaat catcaaatca tcaaatgaac caaagtaaca aaaaagtttt accagctgct
      661 ggtgaaagta tgacatcaag tatacttact gcaagtattg ccgcactact attagtatct
      721 ggtttattct tagcatttag acgacgttca acaaattaat aaacataata cgattaataa
      781 tagaaaaatc gtgtgattat ctgagcgagc ctaggacata aattaatgtc ctaggctcgc
      841 taatgttata ttggcagtag ttgactgaat gaaattgcgc ttgtaacaag cttttccatt
      901 tcttacccac tccctaaata gtcatcaaaa aatttcttat attttaataa ttttaataat
      961 ccgattgtct tatacgtgtc agtgttaatt cagatatttc ctgtggaata taccacttat
     1021 taatcataat tggataaggt gtttgtgcgt acaatgtttc aataatcagc caacaatgtg
     1081 tatcaccatc aaacacgtga ctatgatttt tgaagtgggg tgctttggta atagacattt
     1141 ttaaatctga ttgatatgca ttgttataaa tcgtttgctc aacgaacgtc ttcatgtcgt
     1201 cttcgttttg tgtattcact ttaaatgtgt caatgacatt taacggtata aaggtaaagc
     1261 aaaatgcatc agcttgctta gaatgattgt cctttttttg ataatagcgt tccattgcaa
     1321 tgacggcaga aggatggttt gcaaacaaat gatttgtata ttcactttct aaatcaacac
     1381 gataattaat tgatgacata gatacgcgag ctagcaatat ttgatcaagt ggatgcttaa
     1441 attgatccat acttgaagcg tgttgggcat ttgtttgtgg aataacaaag tgtcccttcc
     1501 ctcttgtact ctctacgatg ccatcttcgg ctaacaattt tatagcttgg cgcaaagtca
     1561 tacgactgac atcaaagcgc gcacaaagtt ccttttcagt aggtaatgca tggccactcg
     1621 gatattttcc tatttgaatt tctttatata acgtattata aatcgttaaa aattttggtt
     1681 gtgtttgcgt cacgtagaca acctccataa agttacttaa tcactctcat catacaataa
     1741 tttttactcg aattggaaaa attataaaaa ttaaatatag ataggctttg aaaattagtt
     1801 ttatacaagg ttagtagctg taactgtaaa atgttcttaa tattgtcaaa atgtaatgct
     1861 tgaaagcgct tttaaaaaat attattatat acatggttag acaaatagac aaatcactat
     1921 acaaatattg ggaggaatat tttatgaaat caacaccaca cattaaacca atgaatgacg
     1981 tcgaaattgc agaaacggtt ctattgccag gagatccgtt aagagctaag ttcattgcag
     2041 aaacttattt ggatgatgtg gaacagttca atacagtgcg aaacatgttt ggttttaccg
     2101 gaacatataa aggtaaaaaa gtttctgtca tgggttcagg tatgggtatg ccatctattg
     2161 gcatttactc ttatgaatta attcatacat ttggttgtaa aaaattaatt cgcgttggct
     2221 cttgtggcgc gatgcaagaa aacattgatt tatatgatgt gattattgcg caaggtgcct
     2281 ctactgattc aaattacgtt caacaatatc aattaccagg tcattttgcg ccaattgctt
     2341 cttatcaatt attagaaaaa gcagttgaaa cagcacgtga caaaggtgta cgtcatcatg
     2401 taggtaatgt gttatcaagt gatattttct ataacgcgga tacaacagcg agtgaacgtt
     2461 ggatgcgtat gggtatttta ggtgtagaaa tggaatcagc tgcattatac atgaatgcaa
     2521 tttacgctgg tgtcgaagca ttaggtgtgt tcacagtgag cgatcattta attcatgaaa
     2581 cgtcaacaac acctgaggaa agggaacgtg catttacaga tatgattgaa attgcactgt
     2641 cattggtgta gatgattatg aatgttgaat attctaaaat aaagaaagca gtacctattt
     2701 tattattctt atttgtattc agtttggtta tagacaactc atttaaattg atttctgtag
     2761 ccattgctga tgacttaaac atatctgtaa cgacagtaag ttggcaagcg acattagccg
     2821 gtttagtaat tggtattggc gctgtagtat acgcttcatt atctgatgcc attagtatac
     2881 gcacactatt tatttatggc gtgatattaa tcattatcgg atcaattatt ggttacattt
     2941 tccaacatca attcccatta cttttagttg gacgtattat tcaaactgcc ggtttagctg
     3001 ctgcagagac attatatgtg atatatgttg caaagtatct ttctaaagag gaccagaaga
     3061 cttaccttgg cttaagtacg agcagttatt ccttgtcatt agttatcggt acattatcag
     3121 gtggatttat ttctacgtat ttacactgga caaatatgtt tttaattgca ttaatcgtag
     3181 tatttacgtt gccattccta tttaaattat taccaaaaga aaataatacg aataaagctc
     3241 atttagattt tgttggctta attctagtgg caactattgc tacaacagtc atgctgttta
     3301 ttacgaactt taattggtta tatatgattg gtgccttaat tgcgattatc gtttttgcgc
     3361 tatatattaa aaatgcgcaa cgtccattag taaataaatc atttttccaa aaaaaacgtt
     3421 atgcttcatt tttatttata gtatttgtaa tgtatgctat ccaattgggt tatattttta
     3481 cgttcccatt cataatggag caaatttatc atctgcaact agacacaaca tcactgttat
     3541 tagtaccggg ttatatagta gcagtcattg ttggtgcact aagtggtaaa atcggcgaat
     3601 atctgaattc aaaacaagcg attatcacag caattatttt aatagcactg agcttgattt
     3661 tacctgcatt tgcagtaggt aatcacattt caatcttcgt catttctatg atattctttg
     3721 caggtagctt tgctttaatg tatgcacctt tacttaacga agccattaaa acaatagatc
     3781 ttaatatgac aggtgtggct attggttttt ataatttaat tattaatgtg gcggtatctg
     3841 taggtattgc gattgctgcg gctctaatcg attttaaagc attaaatttc ccaggcaatg
     3901 atgcattaag ttcacatttc ggtattattt taattatttt aggtttaatg agtattgtcg
     3961 gattagtttt attcgtcagc ttaaatcgtt ggacacaatc tgaaaaataa atagatataa
     4021 attcgcgaga tatattcgta tttatagtaa aattaaataa agagattata taacacgagg
     4081 agtagtaagt atgaaatttg agaaatatat
//
LOCUS       DAHHYJ010000175         3991 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000175 DAHHYJ010000000
VERSION     DAHHYJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.178"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            7..183
                     /locus_tag="NBK22_002422"
     CDS             7..183
                     /locus_tag="NBK22_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415579.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSNDRRKNSK"
     gene            442..822
                     /locus_tag="NBK22_002423"
     CDS             442..822
                     /locus_tag="NBK22_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2415580.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVAPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            819..1715
                     /gene="pmtA"
                     /locus_tag="NBK22_002424"
     CDS             819..1715
                     /gene="pmtA"
                     /locus_tag="NBK22_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCD2415581.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYSENYFNERWTTKQLEKMIAPF
                     YRKWDHQVFEFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGAIEDLDDELASLLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHESIKLMEEGF"
     gene            1716..2396
                     /gene="pmtB"
                     /locus_tag="NBK22_002425"
     CDS             1716..2396
                     /gene="pmtB"
                     /locus_tag="NBK22_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCD2415582.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEADVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLMNYFVLSQSSFPDLYSYILNIGFLIISIVILIVNYFKQLNKINT
                     RKFKGGSR"
     gene            2393..3265
                     /gene="pmtC"
                     /locus_tag="NBK22_002426"
     CDS             2393..3265
                     /gene="pmtC"
                     /locus_tag="NBK22_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCD2415583.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
     gene            3265..>3991
                     /gene="pmtD"
                     /locus_tag="NBK22_002427"
     CDS             3265..>3991
                     /gene="pmtD"
                     /locus_tag="NBK22_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCD2415584.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFALIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLV
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVA"
BASE COUNT         1409 a          491 c          667 g         1424 t
ORIGIN      
        1 aataaattga aacatctaac aaaaatattt gtagtacttg caattatttt atttattatt
       61 ggttattatt tacaagcaac taatcatgaa agccaaggta taaaattatt attagcagcg
      121 attatgttta tgatttgtgc ttttataagt agaagtaatg atcgacgtaa aaatagtaaa
      181 tagaattgac tgtttaaaac gattgtttat gttgacgcta cggttgtcac gtagagatta
      241 aagacattcg ttttagcagt cttttttatt gaagtagaca atgcaagatt aaatggtagt
      301 gtcatttcat tttattcaac tgctagtaat acaatactca gtgttatttc tgaagactgg
      361 ttgataatga gacatgcata tttacaaaac tgtgtatatt gtgtatattg tatatataca
      421 gaaagtgata gggggacgtt gatgaaaata attttaaaaa acaatagtga ttttccgatt
      481 tatgaacaga ttaagcaaca agtaaaacaa aatattttaa agggacatgt tgctcctgga
      541 gagcatttgc cgtcaatgag agaacttgcc aaagatcttc aagtaagttt gattactacc
      601 aaacgtgctt atgaagattt agagaaagac ggttttgtta caacaattag aggaaaaggg
      661 acctttgtta aggagcaaga tagttctatt ttaaaagaga aacaattttt taccattgaa
      721 aatttggtta aagaattggt taatgaagcg caagccatcg aaatgtcact tgaggaactt
      781 caagatattt taacgttcat ttatgaggag gaatcatcat gaatgccata gaattaagta
      841 atgttaatta ttcaagtgat cagtttaatt taaagaatat ctctttcaaa gttcctcaag
      901 gattcgtcac tggttttatt ggtagaaatg gcgctggtaa aaccacaata ataaggttaa
      961 ttatggattt gtatcaacca caaactggtg ttattagagt attagaagag gatatggctc
     1021 tcaatccgat agagttaaag aatagaattg ggtttgttta ctctgaaaat tattttaatg
     1081 agagatggac tacgaagcaa cttgaaaaaa tgattgcccc tttttatcgt aaatgggacc
     1141 atcaagtatt tgaattttat cttgaaaagt tcgatttacc aatcaataaa tcaataaaaa
     1201 cattttcgac tgggatgaaa atgaaattat cactagcagt tgcattttca catcatgctg
     1261 aattgtatat ttttgatgag cctacttcag gtcttgatcc cttagctaga aatgagctat
     1321 tggagataat tcagcaagaa ttaattgatg aaaataaaac aatattcatg tcgacacaca
     1381 ttatctcaga tttagaaaaa atagctgatt atatcattca cttaagtgac ggtgaagtta
     1441 tcttaaatgg ttcaaaagaa caactgttac aaagatatca agttgtgagt ggtgctattg
     1501 aggaccttga tgatgaatta gcatctctac ttatttatga agaacataag cgtacgggat
     1561 ttataggatt aacagaacat gcgcaagtat ttaaagaaat tttaggtcat aaagtgaata
     1621 tcactacacc ttctattgag aacttaatgg tttatcttga aaagagaaaa cctaaatatc
     1681 atgaaagcat caaattgatg gaagaaggtt tttaaatgaa ggcattgtta attaggaatt
     1741 ttaaattacg acgatataca cttatcatct atgttctatt attaacctta tatccttttt
     1801 atataatgct tgactcaaca aagttctttt acctattgca atcttttata agtccaacaa
     1861 tattgattat ttggatttta gatgctggtc acttatttag actcaatcgt agattaggtg
     1921 gtaatgattc atattatttt tatatgagtt taccagtgtc aaaaaaacaa ttgcttaatg
     1981 ccaactatat tacatgcatt gttttaacat taattggaac gcttgttatt agtttatatg
     2041 cttatgaagc agatgtgatt gaaccaaatt caatctattt ttcaactgca tatgcatttg
     2101 tcatatctaa cttcttgtct ataccaattg catttagtca atttacagaa ttgcgtagag
     2161 tcaaagtgcc atatggtata tacgtgttta ctattatcat tttagttcca tttttatttt
     2221 caattgcaat agtgttgatg aattattttg ttctaagtca atcatcattc ccagatctat
     2281 attcatatat tttaaatatt ggttttctaa ttatcagcat tgttatactt attgttaatt
     2341 attttaaaca gctcaataaa ataaatacta gaaaatttaa aggaggcagt cgatgaaatt
     2401 agaacatatt acaaaaaaat acggctcaaa tgtcgtttta aatgatattg attttgactt
     2461 tggcgatagt agaattgtcg gattaatagg aaaaaacggt gttggtaaaa caaccgttat
     2521 gaaagtaatg aatggtaata ttattaaatt tgatggaaaa gtagacattg ataatgcaga
     2581 taatatcggt tttttaattg agcatcctaa attatatgat aataaatcag gattgtataa
     2641 cttgaaatta tttgcacaag tattaggtaa gggttttgat aaagcataca cagacaaaat
     2701 tatagatgca tttggtatga gaccttatat taaaaagaaa gttaagaaat attcaatggg
     2761 gatgaagcaa aagttagcaa ttgcagtatc tttaatgaat aaacctaaat ttttaatctt
     2821 ggatgagcct acaaatggta tggatccaga tggctcaatt gatgtgctga ctacaattaa
     2881 gtctttagta aatgaacttg atatgagaat tctaatatca agtcataagt tagaagatat
     2941 tgaattaatt tgtgatagag ctgtattttt aagagacgga cattttgttc aagatgtaaa
     3001 catggaggaa ggtgttgcat ctgacacaac gatagttact gttgatcata aagactttga
     3061 tagaactgaa aaatatcttg cagagcattt ccaattacaa aatgtcgaca aagcagacgg
     3121 acatttaatg atcaatgcac aaaaaaatta tcaagttata ctaaaagcat tatctgaatt
     3181 agatatttat ccgaaatata ttgaaacacg taaaagttca ttgcgtgata cgtacttcaa
     3241 tataaatcaa agaggtgata aataatgaga attttaaatt tagttaagta tgatttttat
     3301 agtattttta aaagtccttt aacatattta gcgatactag tcgtatctag tttgattgca
     3361 actcaaagta tacttatggc aaattcgatg gataacccga aacatattat tgtctatgga
     3421 tctgtatttg ctgcagcaaa atggttattg ttaataattg gtttaatgtt tgttgttaag
     3481 acaattacgc gtgatttttc acaaggtaca attcaactat atatgagtaa agttaaaaca
     3541 cgcgttggat acattatttc gaaaacaatt tcaattattt taatttcaat attatttgca
     3601 ttaattcatt atgtgatttt gattgttgtg caggcatcta gtaatggaaa aaatttggcg
     3661 ttttctaaat atgtagataa tttatggttc ttcctaatct ttttactatt ctttggcttg
     3721 tttttattct taatcacact tgtatcacaa aaaacagcaa tgatattttc attaggtgta
     3781 tttttagtac tcattgtacc gtttattaaa ccttttatta catttatccc aagatacggt
     3841 gaaaaagttt tagatgcttt tgattatatc ccttttgctt acttaactga taaaatgatt
     3901 agctctaact ttgattttag caattggcaa tgggtaattt cattaggttc tatagtgata
     3961 ttcttcattt tgaatatctt atatgtcgct a
//
LOCUS       DAHHYJ010000176         3907 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000176 DAHHYJ010000000
VERSION     DAHHYJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.179"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            147..3299
                     /locus_tag="NBK22_002428"
     CDS             147..3299
                     /locus_tag="NBK22_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCD2415585.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQRAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQVYHLDNETLRDNKEIEDLDNRINYIVKQIETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLSTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            3356..3838
                     /locus_tag="NBK22_002429"
     CDS             3356..3838
                     /locus_tag="NBK22_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415586.1"
                     /translation="MEKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIEALIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKR
                     "
BASE COUNT         1531 a          486 c          726 g         1164 t
ORIGIN      
        1 ttttattagt gaggatttta caaacatttg ttgaataaat atggtaatga taatatatga
       61 atatttttga aaaaacacta atatgattaa attatttgtt aaaatggcat taagtaaaaa
      121 acaaacaggg aacaaggtgg gatttcatga gtcaattgct aaatgatacg ttatcggctt
      181 ggttgttaat tgaatcttta agtccaggag aagtaaattt tacagcggaa gatatactct
      241 cagctgaaca ttttaaaaat ggtgcaaagc aagcacaact tcaaagtttt gatgaatatt
      301 ttgaaatatg gaatgctgaa cgctttatta tatcagaaga aaaatcagag actggggaac
      361 ttatatttaa attttataga cattgcttcc gctataatga aattaatttg aaaattcagg
      421 atatttttga tgattattct gagattcata atccaaatgg gacacactgt tatggttaca
      481 catttaacac agataaacac ggcaaagtga tagttgattc tatacatatt ccgatgatta
      541 tgagtgcatt aaaagaaatt gaaaagaaca aaaatgccaa tatagaagaa aaatttaatg
      601 attctgttga aaaatttgtt caaaaagtaa aagaaatttt agcagatgaa ccaattaatg
      661 aatttaaatt gaagaagatg gacaaagctt atgatgagta cttttctgta ttaaattcaa
      721 agaaagatgg attatttgga cattatgtag caatagaata tgtgaaagat agtgatttac
      781 cacagccgga atttaacagc ttcttcataa gtgatattga gaaagcaaga aaatctccca
      841 accaaacttt aattgattac attgaaggtg tagaagaaag tcagcgcata gaagtagatg
      901 aaaataaaga aatgtttgac aaatttttac atccttcacg tttgcctgat ggacgatggc
      961 catcacagac tgagtttaga ttgtctttaa tgcaacaact tgctgtaaac caaattacga
     1021 gtggtaatga aagaataagt tcagttaatg ggccaccagg tacaggtaag actactttat
     1081 taaaagatat atttgctcat ctagtagttg aaagaggtaa agagttagct aaactaaata
     1141 atcctaaaga tgcatttgtc aaaacaaaaa ttcatgaaac ggatgataaa tacgtatact
     1201 tactaaagga atctattgcc aaatataaga tggtagtcgc atctagtaat aatggagctg
     1261 ttgaaaatat atctaaagat ttaccgaaaa ttgaagaaat tataagaaat cccgaaaaat
     1321 gtaaattccc taaatatgaa cagaattatg caaatttagc acatgaatta aaagattttg
     1381 ctgaaatagc tgaagatttg attggtgaaa gtgcctgggg cttattttct ggagtttttg
     1441 gtaaaagtac taatattaac caagtattga gtcatatgtt aaaacaagat gcgaatgata
     1501 ttggctttgc taaattacta caaaatgaga ataatcgtat gagttataac gagttaatga
     1561 gtgaatggca atcacatcaa cgtgcatttt tagaagagtt gaggcatgtt gaaatgttaa
     1621 aagaagaatc tattagagca tatgatgttt ataaaaattg tgagtctttc tctaagattg
     1681 aacaggttat taatagtgaa aaaacaagta ttgaagaaca ggtatatcat ttagataatg
     1741 aaacgttacg agacaataaa gaaatagaag atttggataa tcgaattaat tatattgtta
     1801 agcaaataga aactttaaat gagttaatta aatccatcaa agaaagcaac aaaggtttta
     1861 ttaacaaact gaaagcgatg tttaattcag aagaagatga aagctataaa gatcataata
     1921 aagagaagca acaattatta acacaacagt tagagttaga gaaatgtaaa aaaaacaaac
     1981 atgaagacct tgttagcaaa ctaaaagaaa aagagaaatt aattaaacaa ttaactaaag
     2041 tacagttgca attagacgag ttaaattcac agttacaaga gttagaagca tatcgtattg
     2101 agtcaaaaat tacaattcca gaaaaagatt tttggagtga caacaattat gatgagcgcc
     2161 aagttactaa tctgtggacg agtgacgaac ttcaatacag acgtgccatg ctctttttaa
     2221 gagcaatgat attgcataaa ttattattga ttgctaataa tacaactatt tattatgcga
     2281 ttaatgattt taaagataga aggaaattaa ttgatgcaaa tccagataaa gtacacaacg
     2341 catggaatgt gatgcattta atatttccag tagttagtac gacgtttgca agctttaaat
     2401 ctatgtatgg gggcatacca aaagatttca tagactactt atttattgat gaagcaggac
     2461 aagcaatacc tcaagcagct gtgggagcat tatatcgttc aaaaaaagtt gtagctgtag
     2521 gtgatccgat tcaaatagaa ccggttgtga ctttagaaag tcatttaatt gataacattc
     2581 gtaaaaatta tcatgttccg gaatatctag tttctaaaga agcttctgtg cagtctgttg
     2641 cagacaacgc caatcaatat ggtttttgga aatctgatgc tactgatagt aatcaaaaaa
     2701 cctggatagg cataccttta tgggtgcaca gacgatgttt aaaacctatg ttcacgatag
     2761 ctaaccaaat cgcttataat aataaaatgg tgttgccaag taatattaca aaagtaggta
     2821 aaacaggttg gtatgacgtt aaaggaaacg cagttcaaaa acaatttgtg aaagagcatg
     2881 gtgaaaaagt agtgggatta ttagctgatg attggattga agcaattaag gaaggtaaaa
     2941 atgaaccgag ctcatttgta atatcgcctt tttcagcagt acagcaacag attaaacgta
     3001 tgttaaagca acaactatcg actagaattg atattgaacg tacaaaaatt aatcaatggg
     3061 tcgataaatc cattggtact gttcatactt ttcaaggtaa agaggctcag aaggtgtatt
     3121 ttgtaatagg tactgataat acccaagatg gtgctgtgaa ctggtcatgc gaaaaaccaa
     3181 acttgttaaa cgttgcagtg acaagagcta agaaagagtt ttatgtaatt ggcgacatgc
     3241 aaagaataca gatgaaacca ttttatgaga cgatttttaa agaaagaaat gtaaaataac
     3301 atacaaaaaa gtatatagag gaagttatac attttaaaag gagcaaaatt gaataatgga
     3361 gaaatttaac aactggatat taaatgcaat aagtggatct caaacagaca agaatgaaac
     3421 aactgaagaa ttaaaagggg caaaatttat cattttatat gcatattcaa tgctcgtttt
     3481 gcttgcgtta gtaatttcta acatattcat tcacattttg gagcctaaac tatcaatcac
     3541 cactcaaatc atcatcgttt tgattttaat tgaagcacta attggactgc gtttcttgaa
     3601 agcgtacgat gttaagcgtg gcaaagataa agaaaataag aaaaatagta aggatttcgt
     3661 taaactaaaa tcaattttag tagcaatttt atttacatca ttggcgctga cagcaggtac
     3721 tgtagctgat atatacggtt tcactgactt aggaaatact agaagtgatt taatcgtttg
     3781 gagcataggt ggtattatat ttggcctcgt atgttacaca atggaagata aaagataacg
     3841 ataaggagct ggcgattata aagctagctc cttttttaac ttatatatgt aaagaactat
     3901 cctaagg
//
LOCUS       DAHHYJ010000177         3899 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000177 DAHHYJ010000000
VERSION     DAHHYJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.180"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(126..310)
                     /locus_tag="NBK22_002430"
                     /pseudo
     CDS             complement(126..310)
                     /locus_tag="NBK22_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(399..1463)
                     /locus_tag="NBK22_002431"
     CDS             complement(399..1463)
                     /locus_tag="NBK22_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCD2415587.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNALKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            complement(1611..3053)
                     /gene="lmrS"
                     /locus_tag="NBK22_002432"
     CDS             complement(1611..3053)
                     /gene="lmrS"
                     /locus_tag="NBK22_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCD2415588.1"
                     /translation="MAKVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFVFGLVSISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALIISMVIIYLFIRRQFNITNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFLSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            complement(3375..3848)
                     /gene="sepA"
                     /locus_tag="NBK22_002433"
     CDS             complement(3375..3848)
                     /gene="sepA"
                     /locus_tag="NBK22_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCD2415589.1"
                     /translation="MIVNYLKHKFYNLLTTMIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYTVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
BASE COUNT         1344 a          745 c          502 g         1308 t
ORIGIN      
        1 ttttttggaa ttaacgttga catattgtca ttcagttttc aatgttcatt aatgttcaat
       61 ctcttttatt ctacttcatt tatttcttga agtcaataac tttttgaacg attactttat
      121 ttattctata ttgtttttat agttatttca atggtaagtt ttacactttt gaaattcttc
      181 tttaaaaaca actgcgtcgt ttttgacgac tttatcatat tatcaacttt ggaatttaaa
      241 gtcaataact tttttaaaac tttttgtgtt cacaacccgc ttctttttca acgcgtttat
      301 tgcttaacac aagaacttat tttaccagca ttccaaaaca aatcaacata aaaacgtaca
      361 aaataaaagt aattttgtac gtttagcata tattatacct atttatttgt agcagctata
      421 actttttgtg caatcgagct ataaatttta cctagacgat catctgattg atatattgac
      481 ggtgcaaaat cttttggatt ccaagatggt tgctctaaag gtaattcccc aagtaattga
      541 gtattaagtt catcagctaa cttagtaccg ccacctttgc caaagacata ttctttatta
      601 cccgtctctt tactttcaaa ataactcatg ttttcaatta cgccaagaat agaatgatcc
      661 gtatgttttg ccatcgcacc tgcgcgagct gcaacaaatg ctgctgtagg atgaggtgtc
      721 gttacaataa tttccttact tgaaggtaac atcgtatgaa catctaaagc tacatctcct
      781 gttccaggtg gaagatcgag tattaaatat tcaatgtctc cccatttaac ttctgtaaag
      841 aaattcgtca acattttacc taacattggc cctctccata taactggcgc attttcttcc
      901 acaaaaaagg ccattgatat aactttaacg ccatgacgtt caactggaat tacttccttc
      961 cctttaattc caggcttttc atcaataccc atcatatctg gtacactaaa tccatatata
     1021 tcggcatcta ctaatccgac ttttttccct tcacgagcta aggcaacggc taaatttact
     1081 gcaacagtag atttaccgac accaccttta ccggaggcta ttgcaataaa ttcgactgga
     1141 ttattttgag ataataggcc ttctatcgtt ttaggtttat tttcttcttt tggtttaaat
     1201 tgatttactt tttcttccgg caatgtttca aatcgtatac cgaccgtttt cgcaccattt
     1261 tcttttaatg cattaacaac agccatctgt aaatctaatt gcggtgcacc acctaattgt
     1321 gccattgcta atttaacact cacatgttct ttttcttcct taatagaaac ttcaacaata
     1381 ccttctgttt cttttaaagg cacatctata ataggatctt taatttctcc taccaattct
     1441 ttcacttgat ctaccgttaa caaagcaaat cccccttaat cattttaaga catctacatc
     1501 atagcaaata acaatttttg cttagtctat cactaaaagt ataaaacttt ttattaaata
     1561 aaaaaagcag ttagcctatt attttaaata gaccaactac tataattatt ttaaaatttc
     1621 cttctattac tttctatttc tttaggttct cttataaaaa gtgctatcac ataagctgcc
     1681 ataacaagca cagctacaaa agcaaatgag atatccaccc ctatagcaat ggattgctgt
     1741 ttaatttcag ttgcagttaa atgcgatggc gatgtagata aaaatatttt tgcaccaaaa
     1801 ctcattaatg taaccattaa agctgtgcct agtgaaccag ccattacacg accaaagttc
     1861 ataatagccg tgccatgtga gatttcttca tttctcaaag aattaattcc tgtagtattt
     1921 atcggcatca tgagtaaaga aactgaaaac attctaattg catatacgac tattaaatac
     1981 gtataagatg tgtctgtatg caagaaacac aacataattg tagtaattgt taaaattgta
     2041 aaaccagtat aaatcaatgg tctaggacca tacttatcat aaaatttacc tgtaaaaact
     2101 gacataatac catttattat tgcaccaggc atgataacaa gtcctgataa caaggcagat
     2161 aaagataaac tgttttgaac atatagcggt ataagcagcg caggtccaac cattgacatc
     2221 attataatca ttgaactaat cgtacataat gcaaatgttc tatttttaaa aacccttaaa
     2281 tttaaaagtg cattagtaat attaaattgt ctacgtataa ataaatagat aataaccata
     2341 cttattatca acgcacctat tactattgga cttgtaaatc ctaaatctcc tgcactacta
     2401 aatgcgtata gcattaaccc gaatccaata gttgaataca ttactgagcg cttatctaat
     2461 ttcgtatatt tcacttcatt gtaacttgag attgaaacta agccgaaaac aaaggcaact
     2521 aaagcaattc ctacaattat aataaatggt actcgccaac tcgcttgatc aataattaat
     2581 cctgtaactg tagggcctat tgctggtgca aattgaatta ctaaacctgc tagtcccatt
     2641 gcaaaacctc gatgttcttt actgaacaat gtaaataacg taaattgcat taagggcata
     2701 agtacgcctg cacccatcgc ttgaattaca cgtgctaaca ttaaaactcc aaaattcgga
     2761 gctaaggctg caacaataga acctaataaa aatgtcccca tcgccgctaa gtataaaggt
     2821 ctagttttaa ttctatccat tagatatgcc gtcagaggta tcatgacgcc attaacaagc
     2881 ataaacccag taactagcca ttgcgatgta ctttcattga tatttaattc tctcattata
     2941 ctaggtaacg ctgtatttaa taatgtttga tttaaaatag ctacaaacgc actaatcaac
     3001 ataacagcaa ctatgaagtt tcgtctccgg gttgttaatt caactttagc catcataatt
     3061 cctccttttt aaaacagata tacacttgtc cctttaatac gtatagccat gaacataatg
     3121 ggagtagtac tcaaataata aagaagcact acataataaa tcattattgg ttcttacaca
     3181 ttaggcacag ctaatgtgta cttaaaaact aacaatataa cattgtaggg cctaataatt
     3241 tattttatgt ctcagactca tacaatggtt atttaactta tttaccacaa attgagcact
     3301 aagactcact attatcgatt cttctattga acaaaaagaa gttggaacac atatgtgccc
     3361 tcaacttcag atatctattt tctattattt aaatttttac tttcagggta aaattgaagc
     3421 ttacaaacat ataagaaccc tataataacc atgatgaccc aaaaatcgat attgatatac
     3481 acatagttca gatgaataat aaaataaaat aacgttgttg caactgtgta agaaatcaaa
     3541 tgaaaagtcg cgcctctaaa atatgcattt tcaggcaaca tttttctaaa ataatccatt
     3601 aaatgctcga ctgcaaatat gatgaagtac ccaaagatta tataactgcc ataatataat
     3661 aggttatcgt acatacttct attatatgta aagtcaccca acctaaaata aataagtatt
     3721 ctacttaatc catataaacc aaaccctaga aatgttaaaa aaatcgcacc agaaagtaca
     3781 aaaataaaga gaacaatcat tgtagttaac aagttataaa acttatgttt aagataattc
     3841 acaatcattg gcctccttat taataaaata ttgaacgatt tatctagtat ctaggaaat
//
LOCUS       DAHHYJ010000178         3849 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000178 DAHHYJ010000000
VERSION     DAHHYJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.182"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..210
                     /locus_tag="NBK22_002434"
     CDS             <1..210
                     /locus_tag="NBK22_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCD2415590.1"
                     /translation="YNVKQLRKRYNIPTNKAPKLLIKGDGDLKGSSVGSKNLEFTFVE
                     NKEENIYFTDSVQYTPSEDTSYESN"
     gene            complement(214..336)
                     /locus_tag="NBK22_002435"
     CDS             complement(214..336)
                     /locus_tag="NBK22_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415591.1"
                     /translation="MSYIIKFTFKFFEMTTYISQLTLDIELVSSELLVILPGFI"
     gene            771..962
                     /locus_tag="NBK22_002436"
     CDS             771..962
                     /locus_tag="NBK22_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415592.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            complement(1101..1286)
                     /locus_tag="NBK22_002437"
     CDS             complement(1101..1286)
                     /locus_tag="NBK22_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415593.1"
                     /translation="MLYILITALNVILNKLKMKDSNIISFIYSRHDRILYVNLVGNRF
                     KNNISMTKATSNIGRGF"
     gene            complement(1222..1503)
                     /locus_tag="NBK22_002438"
     CDS             complement(1222..1503)
                     /locus_tag="NBK22_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD2415594.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVIYSYNGIKRNFKQVENEGF"
     gene            1792..2487
                     /locus_tag="NBK22_002439"
     CDS             1792..2487
                     /locus_tag="NBK22_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2415595.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKEKLQ
                     NVANQLSQDSVKVVPTDVTNKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            2758..3159
                     /locus_tag="NBK22_002440"
     CDS             2758..3159
                     /locus_tag="NBK22_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD2415596.1"
                     /translation="MIIGVILCICIVASVIYLKVKYDEKEKQKAIYYKEQQERITLYL
                     KHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQFGGSLIK
                     SKNLSTLLKPVHQTKSPDEIKKELESKKNDR"
     gene            3426..3836
                     /locus_tag="NBK22_002441"
     CDS             3426..3836
                     /locus_tag="NBK22_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD2415597.1"
                     /translation="MSKKKILILTSIMLIILISVAGIYFKMKYDEKEKQKSIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTNLETSPMGSAVIEGYINENKKDDFVAYASPENNFQ
                     FVGDLIKSERLSELLKPANQLKSPDDIKKELNKK"
BASE COUNT         1445 a          548 c          642 g         1214 t
ORIGIN      
        1 tacaatgtga agcaacttcg aaaaagatat aatattccaa ccaacaaagc tccgaaatta
       61 ttaataaaag gtgatggtga tttaaaaggc tcatctgtag ggtcaaaaaa tctagaattt
      121 acctttgtag aaaataaaga agaaaacatt tattttacag attctgtaca atatactcca
      181 agtgaggata caagctatga gtcaaactga atatcaaata aaacctggta atataacaag
      241 taactctgaa gaaacaagtt cgatatctaa agtgagctgt gaaatatagg tagtcatttc
      301 aaaaaattta aaggtgaatt tgataatgta gctcaagggg attgggttaa aaaggcgaag
      361 aatgaactgg atgatattag taagaaatta aaaaacattc aaagaactga aatttaatat
      421 cttatatgat tcctgaagct aagacagcat gcgttcattc atgccattat taatataatc
      481 accacaacaa aaaagcttct aatgtgatat tggaacctca aattccgtat catgttagaa
      541 gcttttaatg tctaaagaac acctacattt tatcatattt tcttacttat taaactttta
      601 tataattaac tatttcttaa ttttccaaaa tagtgataaa tttgtgaaat acatcacaaa
      661 cccctttatt tatttggaaa ttcatgtaat attagacttg taagaagtta ataaatagag
      721 agagacgaga gagtttatat aaataatata taaacattgg agtgatgatt atgagaaaag
      781 agattgaagc gcttattttc tcagacgtaa gtagctatga tatttacgtg aacactggtg
      841 taaatcaagg attaattggt gacatcaaag atggttacct aactattgat tctatgcctt
      901 acattgatgc tgagcgtttg tatcactttg ctatggaacg taaatcgtta gtcactaact
      961 agttcttatt gccaatgatt actaccccta gtcggcggca attgaagtgt gattgatgta
     1021 acttgccctc gttggtgagc aattgagggc agaccccttt aattaagtaa accctaactc
     1081 cccacaaatc tggaacgata ctaaaagcca cgtcctatat tggatgtggc tttagtcatg
     1141 cttatattat ttttaaaacg attacctaca agatttacat ataaaattct atcatgtctg
     1201 ctatatataa acgaaatgat attagaatcc ttcattttca acttgtttaa aattacgttt
     1261 aatgccgtta taagaatata taacattata taattttact tgggcgtcat ggacacttga
     1321 gaagggcatt cttacatcgt ccatattttt aacttcgtgt cgaaatgctt tttcagcttt
     1381 ttgtctttct gcaagcgaca tacttagcca ttcttctttg ctaaataaat cttcgaatcg
     1441 aaaatcaaat gcagttcttt tactttctgc tcttaatgct aaaacacgct catgatatgt
     1501 cacgttttat tcctccctct aatcattcaa tcgaatgata aatcacagta ttaactttag
     1561 tactaatata atgtttttac aataaagaag tgacgatttt tcaacatttt taaaaataaa
     1621 aaatataagc aacgattaat tgtatgtttc gaatggcttg taatgtaatt tatctatgaa
     1681 aatgaagcga gttattatga ccgttatatg aattgtgata cgctttttaa aagtaatatg
     1741 tatttatgat ttaccctgaa aaaataaaaa gaatcaaagg agtcgatcat tatgacagta
     1801 ttaacagata aagtagcagt agttacaggt gcaggtagtg gtattggaga agcaattgca
     1861 acattactac atgaagaagg ggcaaaagtt gtcttagcag gtagaaataa agaaaaatta
     1921 caaaacgtag cgaatcaatt gtcacaagat agtgtgaagg tagtgccaac agatgtaacg
     1981 aataaagaag aagtcgatga attgataaaa attgcacaac aaacattcgg tggtttggat
     2041 attgttatca atagtgcggg gcaaatgttg tcgtctaaga ttactgatta tcaagtagat
     2101 gagtgggata gtatgattga tgtgaatatc aaaggcactt tatatacggc acaggctgca
     2161 ttaccaacta tgttagaaca atcaagtggc catcttatta acattgcatc tatttctggc
     2221 tttgaagtaa cgaaaagtag tacgatttat agtgcgacga aagcagcagt tcacactatt
     2281 actcaaggat tagaaaaaga gttggcaaag acaggcgtta aagtaacaag catttctcca
     2341 ggaatggtag atacagccat aactgccgca tacaatccaa cagatcgtaa aaaacttgaa
     2401 ccacaagata ttgcagaagc agtattatat gcattaacac aaccaaagca cgttaacgtg
     2461 aatgaaatta cagtgcgtcc tgtataaaaa ggtgtttgag gtctggggta taggcattta
     2521 atgtaatagc tatcgatttt gttaatgtta gtaaggcaat aattacatga gggcataata
     2581 ggcaattatt ccttgtacaa ttctcagtaa ttggtgattt taacaatcaa gttaatgaag
     2641 gcgacgatgt tagtgttatg gtttataatc tgtaatgttc ataattatag attataaaaa
     2701 ttttttcatt ttccaccaca ttttaggagt tactttaatg aacaaaaaac atgttttata
     2761 attattggtg tcattttatg tatatgtata gttgcatcag tcatttattt aaaagtgaaa
     2821 tatgacgaaa aagaaaaaca aaaagcaata tactataaag aacaacagga gcgtattaca
     2881 ctctatctta agcataatac taaagaaccc aataccatca aaactgtaca tttcacaagt
     2941 ttaaaaagag gacctatggg cgatgcagta attgaaggtt acataaatga aaataaagaa
     3001 gatgattttg ttgcatatgg gtctccagaa cataattatc aatttggtgg aagtttaatc
     3061 aaaagtaaaa atttaagcac gttattaaaa ccagtacatc aaaccaaatc acctgatgag
     3121 ataaaaaagg aattagagtc taaaaagaac gaccgctaaa actatgcggc cgttttaatt
     3181 ttgttttcaa gttaatcata cttacatata aaaatgaaaa gttgaatttt tagtacaatt
     3241 tccactgtat ttatctgtta accactttat acccgcaata tttcgttgat cttatataaa
     3301 cacttttggc tgcttttcct tgacctagat taaaattata gattttcttt gtaagagtgt
     3361 gttttgtagt atcataaata tgtaaactgt ataaaattcc accacatttt aggagctggt
     3421 gatagatgtc gaaaaagaaa attttaatat taactagtat tatgttaatc atattaatta
     3481 gtgttgcagg tatctatttt aaaatgaaat atgacgaaaa agaaaaacaa aaatcaattt
     3541 attataaaga acaacaagcg cgcattacac tttatctcaa gcataacact aaagaaccga
     3601 acacgattaa atctgtgcat ttcacaaact tggaaacaag tcctatggga agtgctgtga
     3661 ttgaaggtta cataaatgaa aacaaaaaag atgattttgt tgcctatgca tcacctgaaa
     3721 ataactttca atttgtaggt gatttaatta aaagtgaaag attaagtgag ttactaaaac
     3781 cagcaaatca attaaaatca ccagatgata taaaaaaaga actaaataaa aagtagagtc
     3841 actaaagtt
//
LOCUS       DAHHYJ010000179         3825 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000179 DAHHYJ010000000
VERSION     DAHHYJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.183"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(285..1931)
                     /locus_tag="NBK22_002442"
     CDS             complement(285..1931)
                     /locus_tag="NBK22_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD2415598.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
     gene            2135..2497
                     /gene="mscL"
                     /locus_tag="NBK22_002443"
     CDS             2135..2497
                     /gene="mscL"
                     /locus_tag="NBK22_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCD2415599.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
     gene            complement(2568..3539)
                     /locus_tag="NBK22_002444"
     CDS             complement(2568..3539)
                     /locus_tag="NBK22_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCD2415600.1"
                     /translation="MSQRKLIEQNNLNHYENQLETYNNDLELNEKSIEMEMSRLNLTD
                     DNDINEIIAWRGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYE
                     QKKEKMNTLIDEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKN
                     NKNLTLENFVLIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHS
                     NKSRHISSLSGGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETA
                     LDTLLNLKSTGRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
BASE COUNT         1271 a          670 c          550 g         1334 t
ORIGIN      
        1 attgatttat gtcccagtct gagttaattt tctataaaag tatagttaat tttttatacc
       61 gtcgtacttc actttagctt tgtcaaaccc ctttctatta agttgtcaga aataaaccta
      121 tcttaaaata taaaaaaaat cgagaattcg tagtttaata acgaaattct cgttcttatc
      181 cttttgaata tactcaattt tccacaaaaa caaacaagta gtatatctgt tctagctact
      241 agaatgacat actacttgtt atttaaatac ttaactaaat tttattagtt atcttttttc
      301 tctatatttc tacgtgactg acgcttttca agaatgtcag attcataatc ttcttgttga
      361 ctcttgatat attcttgtaa gcgatgttta ttcggagtca atgttaaacc taggaattta
      421 cgttcctggt tcgcatcctt gtagaaactt accatcatga gtatgacgac aaaggagaat
      481 gggaatgcac ttataattgc agcactttga atcgcattta aagcttcagc gccgttaccg
      541 ccaccagcta ataaaagtac aaatgctatt aaggcctgtg aaattcccca aacaactttt
      601 accatactag atggatttaa tgaaccaaat gttgtttgca ttcctaatac aaatgttgct
      661 gagtcagcag atgtaataaa gaatgatgca attaataata atgcaatcaa cgataaaaca
      721 atgccaaatg gcacatgatt aaacactcca aatagctgtg tttcaggagt catatcaaaa
      781 atttctttgt gtttcttacc tgtctcgatg cctaatacac caaagacact aaaccaaaca
      841 aaactaacaa ttgctggaac tagcaagaca ccagaaatga actctctaat tgaacgtcct
      901 tttgaaactc gtgcaataaa cactccaacg aatgggctcc aacttaacca ccaaccccag
      961 taataaagtg tccatgaaga catccattca cgtttttgag gatttaaagc tgctgtatca
     1021 aaactattaa acaagaatgt gtttagtaaa ctacccgtag agctagttaa catatttaaa
     1081 ataagaacag ttggtccaac aattaaagca gctaccatta aaatagtacc taaaccaatg
     1141 ttcaagttac ttaagtattg aataccttta cttaatccag accatgcact tgctataaat
     1201 aagatagtaa caacaatgat gataatcgct tgtacaaacg tattatttgg aacattgaac
     1261 aagtaatgta aaccaccatt aatttgtaga gcacccatac ctaacgaaac ggctacccca
     1321 acgattgtcg caaatacaga taaaacgtca ataaaaatcc caataggacc ttctacttta
     1381 tcacctaaaa gaggacgtaa agttctagat aataaacctg gttcaccttt acggaattgc
     1441 gaatatgcca acgctaacgc aacaacacca taaacagccc aagcatggaa tccccaatgg
     1501 aaaaatgttg aacgtagagc ttcagtataa gcttcagtag ttttgggatc tgctgtaggt
     1561 ggcgtagcaa agtgcgccat cggttcagct gcaccataaa acaccaaacc tatccccata
     1621 ccagcactaa acaacatagc aaaccatgaa attgtattaa actcaggttt gtcatttggt
     1681 ttacctagtt taagttttcc aataggacta aaaataagga atatacagaa gaacacgata
     1741 atcgtagtaa gaataagata ataccaacct aacttttctg taatccacat tttaatatta
     1801 ttggtaacat agttgaattg ttcaggtaaa aatgcaccaa gtaatacgac tatagcaaca
     1861 acaattgcac tatagatgaa gactggtgaa tacttctttc catttggatt ctctggtgaa
     1921 gaagaattca taattaatta ctcccttcaa ttctatattt aattttatgt agtagaataa
     1981 aaagattatc taaacatttt attcaataac tcacgcacct ttataggata acaaaggtct
     2041 ttgcgataat caaattttct tgaataatct attttctaat ttttttattt gttgtataat
     2101 tgagattaca tttaaaaaag agaggttgag tgtaatgtta aaagaattca aagagttcgc
     2161 cttaaaaggt aacgtcttag atttagcaat tgctgttgtg atgggtgcag ctttcaacaa
     2221 gattatatct tcattagtag aaaatatcat catgccatta attggtaaaa ttttcggatc
     2281 agttgatttt gctaaagaat ggtcattctg gggtattaaa tacggtttat ttatccaatc
     2341 tgttatcgac tttattatca tcgcgtttgc tttattcatc tttgttaaga ttgcaaatac
     2401 attaatgaag aaagaagaag ccgaagaaga agcagttgtg gaagaaaatg ttgtgttatt
     2461 aactgaaatc agagatttat tacgtgagaa aaaataattt ttaaataaaa agagatgggc
     2521 taaggttgtt ctatacattc caaagtacca tctctatttt ttatgtttta atttcttttg
     2581 aatcttgttg aactctgata ttgatctgat ttcacttcta aaactaaagg tattctattt
     2641 ttcaattcgc tcacatgtga aataatccca accattctac cagttgattt aagatttaat
     2701 aaagtgtcta acgctgtttc aagcgtttct tgatctaatg taccgaatcc ttcatcaata
     2761 aatattgatt ctagtgaaat acctcctgat tgctgctgta caatttcgct taaccctaaa
     2821 gctaatgcaa gcgacgattg gaaagtttct ccacctgata acgagctaat atgtctagac
     2881 ttatttgaat gcaaatcaaa tacatcaatt tctaggccac taagaccatg agaaaccgct
     2941 tctcgcctaa ttagttggta tcgattatct gacattgttg ctaatctcag atttgcttgg
     3001 gcaataattt gatctaaata gtaaattaag acaaagtttt ccaatgtaag atttttgtta
     3061 ttcttaccac tgacaatttc agccaattga aaaatttctt gttgatcctt taattcttga
     3121 tttaagtaat taatatgcga aactatagat tgtgttttat taatattatt ttgacattga
     3181 taatgaacag cagagtattc atctatcagt gtattcatct tttctttttt ttgctcatat
     3241 tcatctttta atttatcaga gtccaataac tctttatcct tggttaatga ttctaaccta
     3301 gctatttcca tttcaaattc atgataacgt tttttataag tatccctttt ctgctctaat
     3361 tcctcttgct cgcccctcca ggctattatt tcatttatat cattgtcgtc agttaaattc
     3421 agcctcgaca tttccatttc aatagatttt tcattcaatt ctaagtcatt attgtaagtt
     3481 tctagttgat tttcatagtg atttagatta ttttgctcaa ttaactttct ttgagacaat
     3541 tgcattgact tatcttcaat ctcctttgag tgttcttgat gttggttcac atcttgaata
     3601 tacttcttat aatccacctc aaaattagtg atattattat atttcgagag tgtttcaaat
     3661 tcagtgatga gatctcgaca taatttcaac tcagattcat ttttatttag tcgcaattgt
     3721 ttattatgaa tttgcaacgt tagattatct ttttcttctt tcatttgttc tataaattta
     3781 ttaagatctc tttgattatt caatgcattt tcattttcta gcaga
//
LOCUS       DAHHYJ010000180         3769 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000180 DAHHYJ010000000
VERSION     DAHHYJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.184"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..712
                     /gene="gcvT"
                     /locus_tag="NBK22_002445"
     CDS             <1..712
                     /gene="gcvT"
                     /locus_tag="NBK22_002445"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCD2415601.1"
                     /translation="LKHKEKFDVEVQNVSNQYGQLAIQGPKARDLINQLVDEDVTEMK
                     MFEFKQDVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEYNVMPCGLGARD
                     TLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQKENGAPRRTVG
                     LELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRDEFEMGRELLVQ
                     VRKRQLKAKIVKKNQIDK"
     gene            732..2078
                     /gene="gcvPA"
                     /locus_tag="NBK22_002446"
     CDS             732..2078
                     /gene="gcvPA"
                     /locus_tag="NBK22_002446"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCD2415602.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            2071..3543
                     /gene="gcvPB"
                     /locus_tag="NBK22_002447"
     CDS             2071..3543
                     /gene="gcvPB"
                     /locus_tag="NBK22_002447"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCD2415603.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLLKPMVIKDGDKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
BASE COUNT         1356 a          519 c          740 g         1154 t
ORIGIN      
        1 tttaaaacac aaagagaaat ttgatgttga agtacaaaat gtatcaaacc aatatggtca
       61 attagcaata caaggaccaa aagctagaga tttaattaat caattagttg atgaagatgt
      121 aactgaaatg aaaatgtttg aatttaaaca ggatgtcaaa ttatttggag caaacgtcat
      181 tttatctcaa tcaggttaca caggtgaaga tgggtttgaa atttattgta atatcgatga
      241 tactgaaaaa atttgggatg gtttattaga gtataatgtt atgccatgtg ggttaggcgc
      301 tcgtgataca ttaagattag aggccggatt gccattacat ggacaagatt taactgaatc
      361 aattacacca tatgaaggtg gtatcgcttt tgcaagtaaa ccattaattg atgctgattt
      421 tattggtaaa tctgtattaa aagatcaaaa agaaaatggt gcaccaagaa gaacagtggg
      481 attagaatta cttgaaaaag gaattgcaag aactggttat gaagttatgg atttagatgg
      541 aaatattatt ggagaagtaa cttcaggaac acagtctcca tcatcaggaa aatcaattgc
      601 acttgcaatg ataaaaagag atgagtttga aatgggtaga gagttgcttg ttcaagttcg
      661 taagcgtcaa ttaaaagcga aaattgttaa gaaaaatcaa attgataaat aattaaaaag
      721 gggtgtgcat tgtgagtcat cgttatatac ctttaactga aaaagacaag caagaaatgt
      781 tacaaacaat tggtgcaaaa tctataggag aattattcgg tgatgtacca agtgacattt
      841 tattaaatag agatttaaat attgctgaag gcgaagcgga aacaacgtta cttagaagat
      901 taaatcgcat tgcaagcaag aatatcacta aagaaacgca tacatcattt ttgggtgctg
      961 gtgtttatga tcattacgcg ccatcagttg tggatgctat gatttcaaga tcagaatttt
     1021 acacagcata tacgccatat caaccggaaa tttctcaagg tgaattacaa gccatttttg
     1081 agttccaaac tttaatttgt gaattaacag atatggatgt agcaaattct tctatgtatg
     1141 atgggatgac tagctttgct gaagcatgta tcttagcatt tagtcaaaca aagaaaaata
     1201 aaattgtagt ctcaaaagga ttacattatc aagcattaca agtacttcat acttatgcta
     1261 agacgcgtaa ggaattcgaa gtagtggaaa tcgatttaga tggcactgtt acagatttga
     1321 aaaaattaga agcagcagtg gatgatgaaa cagcagctgt tgcagtacaa tatccaaact
     1381 tctacggttc aattgaagat cttgaaaaga ttcatagctt tattgaagat aaaaaagcat
     1441 tatttatcgt atatgcaaat ccattagcat tgggattact tacacctcca ggttcatttg
     1501 gtgcagatat tgttgtaggt gatactcagc cattcggaat tcctgctcaa ttcggtgggc
     1561 cacattgtgg atattttgca acgacgaaaa agttaatgcg taaagtgcca ggtagattag
     1621 tcggtcaaac acaagatgat gaaggtaatc gtggctttgt attaacatta caagcacgtg
     1681 aacaacatat tcgacgtgat aaagcgacat ctaatatttg ttctaaccaa gcattaaatg
     1741 ctctagcgtc atcaattgca atgtcagcac ttggtaaaca aggtatctat gatattgcag
     1801 ttcaaaatat agaacatgct aactatgcta aacagcaatt tattaaaaaa ggttttgaag
     1861 tattagatgg tacatctttc aatgagtttg tagtcaaatt tgacaaacca attcaacaag
     1921 ttaacgagga attagtgaag tacaatatta tcggtggttt tgatttaggt gttgtttcag
     1981 acgactttaa aaatcacatg ttaatagctg taactgaatt aagaacaaaa gatgaaatcg
     2041 atacatttgt agaaaaggct ggtgagttaa atgactagta aatcaagtcc attaattttt
     2101 gaaagatctc gtgaaggcag atatgcatat tcattaccaa aaagtgatat taaaacaaat
     2161 tctgttgagt cattgttaga tgataaattt attcgtaaaa acaaagcgga gtttcctgaa
     2221 gttgctgaac tagacttagt acgtcattat actgaacttt caaataaaaa tttcggtgta
     2281 gacaacggat tttatccatt aggttcatgt acgatgaagt ataaccctaa aattaatgaa
     2341 aaggttgcta gaataccagg atttagtgaa tcacatccat tacaagatga agaccaagtt
     2401 caaggttcat tagaaattat ttatagtttg caagaagaat taaaagaaat tactggtatg
     2461 gatgaggtga cattacaacc agctgctggc gcacatggtg aatggactgc attgatgata
     2521 tttaaagctt atcatgagaa taatggtgaa ggtcatcgtg atgaagtcat tgtgccagat
     2581 tctgcgcatg gtacgaatcc agcctcagct tcatttgcag gatttaaatc agttactgta
     2641 aaatcaaacg aacgtggcga agttgatatt gatgacttga aacgtgttgt aaatgaaaat
     2701 acagcagcta ttatgttaac taatccaaac actttaggta ttttcgaaaa aaatattatg
     2761 gaaatccgtg aaatcgtcca taatgctggg ggtctattat attatgatgg tgcgaattta
     2821 aacgctatta tggacaaagt tcgcccagga gatatgggat ttgatgctgt tcatttaaac
     2881 ttgcataaaa catttactgg tccacatggt ggtggcggtc ctggttcagg tccagtcggt
     2941 gtagtaaaag aactagcaag ttacttacta aagccaatgg ttattaaaga tggcgacaaa
     3001 tttaaatatg ataatgacat taaaaattct atcggacgtg taaaaccatt ttatggtaac
     3061 tttggtattt acttaagagc ttatacgtat attcgaacta tgggagcaac tggacttaaa
     3121 gaggtttctg aagcagcggt tcttaatgca aattatatta aagcacgttt atctgaacac
     3181 tttgaaatac cttataaaca atattgtaaa cacgagtttg tattaagtgg tgtgcgtcaa
     3241 aaagaatttg gtgtacgtac tttagacatg gctaagcgat tattagattt cggtgtacat
     3301 ccaccaacaa tatacttccc attaaatgtt gaagaaggta tgatgattga accgactgag
     3361 acagagtcta aagaaacact tgattatttt atcgatacat taattagtat tgctgaagaa
     3421 gctaaaaatg atcctgataa ggtgctagaa gcaccacata caactgtgat tgatcgatta
     3481 gacgaagcta cagctgctcg taaaccaata ttaaagtttg aaaatcttaa acaggaaaaa
     3541 taaagtatta aacacatatt ccgagaatta ttattctaac tttgtatgaa gatttaagga
     3601 taatggtttc aaaatcaatt gaaaaagaca atttctattt gaacaagaaa actaaaccga
     3661 agtaataact cttagggttt ggtattattc tttcatagaa attgtctttt cattttttag
     3721 attgcggtaa ttgaatcgta ttgaaaatga gctgaacttt ctttattat
//
LOCUS       DAHHYJ010000181         3725 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000181 DAHHYJ010000000
VERSION     DAHHYJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.185"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(72..305)
                     /gene="nrdH"
                     /locus_tag="NBK22_002448"
     CDS             complement(72..305)
                     /gene="nrdH"
                     /locus_tag="NBK22_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCD2415604.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            503..1864
                     /locus_tag="NBK22_002449"
     CDS             503..1864
                     /locus_tag="NBK22_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCD2415605.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKMSMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            1861..2880
                     /locus_tag="NBK22_002450"
     CDS             1861..2880
                     /locus_tag="NBK22_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCD2415606.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLLVFDKEYV
                     EGKKS"
     gene            3013..3675
                     /locus_tag="NBK22_002451"
     CDS             3013..3675
                     /locus_tag="NBK22_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCD2415607.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     QSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
BASE COUNT         1184 a          498 c          692 g         1351 t
ORIGIN      
        1 atgttagtta atttaaaaat cgatatttaa tttatgatag ctataaatga tgaagttgat
       61 ttagatgaaa attaatcttg gatatttaat actttgttga tttcatcaag atcaacatgg
      121 tacattggat taccattcaa caaaataaac ggagttgaaa aagcatcaaa atctatcatt
      181 tcgtttcgat attgttgatt gttgatattt ctctcttcaa aatcaatttg atgctcatta
      241 agataatttt ttacaaatgt acaaggtggg caatcattct gcgtataaac gattatttct
      301 gacatgtatc atgctccttc gtgttttatt ctaatgtaat atacacaatt atacattgaa
      361 attcaaattt gtgaacacat tgtgaactga cataaattat acacaatttc atagcgcata
      421 atgttttaaa aatacacatt atagcaatta taattaagtt cattgtgttg tcatcaaaaa
      481 aagaaaaagg tgatgttttt aaatggatac agttgaaatc agtcggtttt tgacagctat
      541 gactttagca gttcatatca tttttgcaac gattggtgtt ggtatgcctt taatgttcgc
      601 aattgcagaa tttttaggta ttcgcaaaaa tgatcttcaa tatatagcta tggccaaaag
      661 atgggctaaa gcttatacaa ttactgtagc agtgggagtt gttacaggta caattatagg
      721 acttcaatta tcattgattt ggcctacatt tatggaaatg ggtggacacg ttattgcact
      781 tccattattt atggaaacat ttgcgttctt ctttgaagct attttcttaa gtatatattt
      841 atatacttgg gatcgtttta aaaataaatg gacacatttc ttaattagta taccagtaat
      901 tattggtggc tctttctcag cattcttcat tacttcagtg aattcattta tgaatacgcc
      961 tgcaggtttt gagttgaaga atggaaagat ggtaaatgtt caacctatag aagcgatgtt
     1021 taacccatcg tttatagtta gatcatttca cgtaattact acagctggta tgacgatggc
     1081 atttgttatt gcatcaatag cagcttttaa attattgcgt aatcgtcaac ctaaagatac
     1141 tgtctaccat aagaaagctt tgaaaatgtc tatgattgtt ggattctttt caactttact
     1201 ttctatgttg gcaggggatt tatctgcaaa atttttgcat aaattccaac ctgaaaaatt
     1261 agcagcttat gaatggcatt tcgatacatc ttcccatgct aaattattat tatttggtgt
     1321 gttagatgaa aagactcagc aagttaaagg tgcgattgaa ttacctggac tattgagttt
     1381 cttagcagat aatagtgtca aaactaaagt gcaagggtta aatgattttc caaaaagttt
     1441 acatccgcct atgattgtcc attatttctt tgatttaatg gtaacgatgg gaattttatg
     1501 ttttgtcatt tcaggtgttt atgttttaac tttaatgttt aaaaagctta gaaagttttc
     1561 tactcataaa tggatgcttt acggaatatt attaacagga ccagcttcaa tgctagctat
     1621 agaatttgga tggttcttaa cagagatggg tagacagcct tggattgttc gtggttatat
     1681 gcgcgtggca gaagcagcaa cacaagcagg cggaataacc ttcgttacaa ttttatttgg
     1741 catattgtac atcattttaa tgtatacatg tgcatacgta ttaattcgta tgtttaaaaa
     1801 taaaccggcg tatgaagatg taaatcgttt agccaagaag caaggaggag aaatagaaaa
     1861 atgatttatg catttatagg tataacagtg ttatggttgt ttttattttg ctatatcatt
     1921 attgcttcta tagattttgg ggcaggtttc ttcgcattgc attcaaagtt aactggtgat
     1981 gaaaagaaaa ttaatcactt aatttcacgt tatttaaacc cagtttggga agttacgaat
     2041 gtattctttg ttttcttctt cgtaggattc gtaggtttct ttccagagtc aatcaaatat
     2101 ttaggcacgg tattgttaat accaggttca atagcactga ttatgatatc gttgagaaac
     2161 agtttttatg catttgaaaa ttatggtcaa gatacaaaat tagcatggat gatcatgtat
     2221 ggggtaagtg gattattaat tccagcttca ttatctactg ctttaactat tacagaaggt
     2281 ggctatatta atgttcgaaa caatgttatc gatctagatt gggtgcagtt actattaagc
     2341 ccatttgctt ggtctgtagt attcttggca attatttcag ttttatatat ttcatcagga
     2401 tttttgacat attatgctaa aaaagcaaat gacgaaccag catataattt aacaagacaa
     2461 tggcacatat ttttagggcc gccgatgatt atcatttgtt tattcgtatt tctatcatta
     2521 cgcattcaaa attctgaaca tttttattca gctgtttttg actattggtg gatgtttgct
     2581 ataagtttct tattctttgc attagcttca ttgttaacat tctttaagaa aaaacatggt
     2641 ttggcttttg tatttgttat tttacaaatg atgttcgcgt tctttggcta tggaattagt
     2701 aaattgccat accttttata tccgtttgta aaaattacag atgcatatgt taatccagaa
     2761 atgggctgga cattagtgat tgtctttatt ttaggtttac ttttattact tccatcgtta
     2821 atattattat taagattatt agttttcgac aaagaatatg ttgaaggaaa gaaatcataa
     2881 gttgagacga taccccaact ttcatttact tgcattactg catttagata atttagttgg
     2941 ggtactcatt tataataatt ttaaatttgt attttcagtg aattgatgaa aatcatttaa
     3001 tggagggcga gtatgggtaa agaatatgta gtcatcggtc taggtcgttt tggaggtagt
     3061 atcgttcgtg agttgaatgc attagacatg gatgtaatgg ccatcgacca tgatgaaaat
     3121 agagtgaatg aatatagtga tatcgctact catgcggttg ttgcagacac aacagatgaa
     3181 gcagttatga aaagtttagg tatccgtaac tttgatcatg tcattgtggc aattggtgaa
     3241 aatattcaat caagtacgtt gacgacttta attttaaaag agttaggtgt aaaaaaagta
     3301 actgctaaag cacaaaatga ttatcatgca aagattttaa ataaaattgg agcagatacg
     3361 gttgtgcacc ctgagcgtga tatgggtaga cgtattgcgc ataatgttgc gagtgcaagt
     3421 gtacttgatt atcttgagtt ggcagacgag cattctattg tagaattgaa agcaactgaa
     3481 aagatggcgg ggcagtctat cattgattta gatataagag cacaatatgg aattaacatt
     3541 attgcaatta aacgaggcaa agagttcatc atttcaccaa atccaaatat taatttagaa
     3601 ataggtgata ttttaatcat gattggacat gataatgatt taaatcgctt tgaaaaaaat
     3661 attgcgacga gataatcggg agtgggacag aaatgatatt ttctcaaaat ttatttcgtc
     3721 gtccc
//
LOCUS       DAHHYJ010000182         3631 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000182 DAHHYJ010000000
VERSION     DAHHYJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.186"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(402..475)
                     /locus_tag="NBK22_002452"
     tRNA            complement(402..475)
                     /locus_tag="NBK22_002452"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Arg,seq:tct)
     gene            839..973
                     /locus_tag="NBK22_002453"
     CDS             839..973
                     /locus_tag="NBK22_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCD2415608.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            1030..1164
                     /locus_tag="NBK22_002454"
     CDS             1030..1164
                     /locus_tag="NBK22_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCD2415609.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            1288..1983
                     /locus_tag="NBK22_002455"
     CDS             1288..1983
                     /locus_tag="NBK22_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCD2415610.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(2092..2532)
                     /locus_tag="NBK22_002456"
     CDS             complement(2092..2532)
                     /locus_tag="NBK22_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD2415611.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            2727..>3631
                     /gene="bshC"
                     /locus_tag="NBK22_002457"
     CDS             2727..>3631
                     /gene="bshC"
                     /locus_tag="NBK22_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCD2415612.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQISFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHF"
BASE COUNT         1300 a          510 c          635 g         1186 t
ORIGIN      
        1 taattataat tataggattt cgggcccctg aaaagtccct aaaattttga atagtatcaa
       61 aatctatagg aataatataa taataaaaat ctctacgcat gcaaatggtg agtgttaaaa
      121 atcttggtgt agtgttggtg tagttcaggt gcagtataga tgaaatttga gcacaaaatt
      181 acttaattaa aaatgtataa acaagtgtca gacgcgtgtc aattgtgtgt caaaaaattc
      241 tataacgaat aagcatgctt gttttaatta ctgtattttc ggggcttatg cgggggacgt
      301 tttggtgacg catcatacta tattactgaa attcaaaaca aaaagagccc tgtaatcacg
      361 gaactctttt gtttggtaat gcgtataaaa atacctataa acgtcctggg agggattcga
      421 acccccgacc gatggcttag aaggccattg ctctatccag ctgagctacc aggacacgtt
      481 taacaacaca agaattatta tatctaaatg aacttaaatt agcaatacct tgtaaataaa
      541 aaatgtttat atttttcact attatagagc tatttatcta aaaaggttca ataagactta
      601 aatacgaatt caggcaactt aattgtgtta aatacagttt tgaatgccta actgtatttc
      661 ttttctcttt aaaatacagt taagtacatt ataagatgtt gtgcggataa acaaactaat
      721 tgcatcaaat ttattttaaa ataacaacaa caaaacgtta agcgaataac atttcggtga
      781 tttaaaagct acgcacgttt ttgttatctt caaatttaaa ttttaaggag tgttttcaat
      841 ggaaggttta tttaacgcaa ttaaagatac cgtaactgca gcaattaata atgatggcgc
      901 aaaattaggc acaagcattg tgagcatcgt tgaaaatggc gtaggtttat taggtaaatt
      961 attcggattc taatttcaat atgtaatgta agtaatcagt attatttcaa aggtgaggga
     1021 gagatttaaa tgactggact agcagaagca atcgcaaata ctgtgcaagc tgcacaacaa
     1081 catgatagtg tgaaattagg cacaagtatc gtagacatcg ttgctaacgg tgtgggttta
     1141 ctaggtaaat tatttggatt ctaatataat aactaatatt ctttaaaata aactgggtga
     1201 gcatacttta atgttatgca ctcagtttat tttatttgca gaaatttgag cctctgttaa
     1261 gatttagata catagacaat ataggagatg gggaaattgg gatataaaaa tattttgata
     1321 gactttgatg atacaattgt tgatttttat gatgcagaag aatgggcgtt tcactatatg
     1381 gcgaatgttt ttaatcataa agcaacaaag gatgattttt taacatttaa aaaaatcaat
     1441 caccaacatt gggaagcttt tcaacaaaat aaattaacga agtctgaagt attatcagaa
     1501 cgatttgtga attacttcaa acatcatcaa atggaagttg atgggcatcg tgcagatgtg
     1561 ttatttagaa atggattagc agaagctaaa gttaaatact ttgatcaaac attagaaaca
     1621 attgtcgaat tatcgaaaag acatgattta tatattgtta ctaatggtgt aaccgaaacg
     1681 caaaagagaa ggttaaatca gacgccgttg cataaatata ttaaaaagat atttatatct
     1741 gaggaaacag gatatcaaaa acctaatccg gaatttttta attatgtttt taatgatatt
     1801 ggtgaggatg aaagacagca ctcgattata gttggagatt ctttaacatc tgacattcta
     1861 ggtggaatca atgcgggtat agctacttgc tggtttaatt ttagaggatt tgatcataat
     1921 ccaggaatta tacctgatta tgaaattaat tcatggaaac aactaaatga tattgtacgt
     1981 taagacaaat aatttgaaaa tggtttactt atattagctt aagaaggtaa ttgtaaaaaa
     2041 gacactacga aaagatgcat tttcatgctc gtagtgtcga atattattat cttatggttt
     2101 ccacaataag acatcatgtc cctctatatt tttagcttcc acatctgtaa atccatgatg
     2161 taaaaagaaa tccttagaat catttctgcc aatggcttta attggcatat tgaaactctt
     2221 tgcaaaatca atcaattctg aagcgtaccc tctgttttga tattttggta atacttctaa
     2281 cttccataat aatatataat cttcaaaatc tgggaagtag atttcttcga catcaccttt
     2341 ttttagtaat gccattctag ctcctaattg atctccgaca aatatgccat aaaatggtga
     2401 atctgaactt gcatcaatca tttgaccgtt taactcattg accatgtata agtctttgtt
     2461 gccaaacgct ctaaagtttt cgaataattc gtcagttttg taattaattt caagacgttt
     2521 gatttcactc atatttatct accccttatt tgtttactac tattattata cattaaacca
     2581 cttgtgtttt tcattgattt gaaaatgtaa aacaatgaat attataaatt ttccatgatt
     2641 aaattgattt tcaagccgta aaattgtcaa tcaccggtat agactttata ctatagtttg
     2701 taatcgcgta cttaaggaga aaaaacatgg actgtaaagt agttagttta aatgaaaaag
     2761 atcagtttat accaaaaata aagagcagtg accctgtaat aacaggatta tttcaatatg
     2821 atgcagctca acaaattagt tttgaaaaaa gaatgtctaa agaaaataat ggaagagaag
     2881 cggcattagc gaatgttatt cgtgaatata tgagtgattt aaagctttct agtgaacaag
     2941 aattaaacat acaacattta gctaatggtt caaaagttgt gattggtgga caacaagcag
     3001 ggcttttcgg gggaccattg tatacattcc ataaaatatt ttcaatcatt actttatcta
     3061 aggaattaac ggatacacat aagcaacaag tagtaccagt tttttggatt gcaggagaag
     3121 atcatgattt cgatgaagtg aatcatacat ttgtttataa cgaaaatcat gggtcgctgc
     3181 ataaggttaa atatcataca atggagatgc cagagacgac tgtctctaga tattatcctg
     3241 ataaggctga gttgaaacaa actttaaaaa cgatgttcat tcatatgaaa gaaactgttc
     3301 atacacaagg tctactggag atttgtgaca gaattattga ccaatatgac tcgtggactg
     3361 atatgtttaa agcactactg catgaaacat ttaaagcata tggcgttcta tttatagatg
     3421 cgcagtttga gccgttaaga aaaatggaag cgcctatgtt taaaaagatt ttgaaaaaac
     3481 atcagttgct tgatgatgct tttagagcaa cacaacaacg tactcaaaat caaggcttga
     3541 atgcgatgat acaaacagat acaaatgttc atttattctt acatgatgaa aatatgcgtc
     3601 aattagtttc gtatgatggt aagcatttta a
//
LOCUS       DAHHYJ010000183         3549 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000183 DAHHYJ010000000
VERSION     DAHHYJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.187"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(136..1263)
                     /locus_tag="NBK22_002458"
     CDS             complement(136..1263)
                     /locus_tag="NBK22_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD2415613.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPGGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNRRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISYMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFVEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1625..2002)
                     /locus_tag="NBK22_002459"
     CDS             complement(1625..2002)
                     /locus_tag="NBK22_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCD2415614.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKRAIRND"
     gene            complement(2417..3010)
                     /locus_tag="NBK22_002460"
     CDS             complement(2417..3010)
                     /locus_tag="NBK22_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD2415615.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            3074..3457
                     /locus_tag="NBK22_002461"
     CDS             3074..3457
                     /locus_tag="NBK22_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCD2415616.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
BASE COUNT         1127 a          648 c          499 g         1275 t
ORIGIN      
        1 atgattacaa gcaaaagaat tgataatttt acacttaatc aaaagtatat tttactaaga
       61 atatttttat ttataaatat tgaaaaccac taacaaattg catacacaat atcattagtg
      121 gtaacagtta aacacttatt tatctttacg tggtaatgga tagaatcctt cattaaattg
      181 atgccataaa ttaggagtta agtgatgatt atcttctctc tctggatcaa agtaattaat
      241 aatttcatct tcccgtccat ctttaatttt ttcaacaaat tgataatcca gtagtagctc
      301 tctaccaatg gctactaagt caacaccaac attttcaact gcatctaaag cttcgtcagc
      361 tgtgaaaatt gaaccaatac cgataagtgg catacgacca tttatccatt tgtgaattaa
      421 aggcagtctt tcttgtccag cgtatttacc ttcacgtgtc gttgcatgcg tatccatcat
      481 cgaaacatga atatagtcga ttggcatata gctaatttta ttaacgagtt cctctgtaat
      541 ttccattgtg attcctggag actccgcttc ctctggagat aatctgtatc caactataaa
      601 gtctttattg ccatacgctt ctttcgcttt aagcacctct tcaatcacag cgaccgggaa
      661 tttatattga tttgcccaaa catcatttct tctattatag tatggtgata caaattgatg
      721 aattaagtaa tgattcgcgc catgtatttc aacaccatca aatcctgctt caattgcacg
      781 tcgcgttgct tcaccaaaat ccttgattgc ttgttcaatc tcttcattcg tcatttctct
      841 agcactatgt tcttgtttct gaccgaaact ttttaaagaa attggacttg gtgctacgac
      901 gtctccacca ggtgttaatt caggcaatgc ttgtgcaccg ccatgatgta tttgtacgag
      961 tgctttggca ccgtttttct tcattgctgt agctaatcgt tttagtcctt caatatcact
     1021 gtcatgcgcg attgatggct gtcctggaaa tgcttttccg acatcactca cattactcgc
     1081 agcattaatt gtaataccaa catcttgtga acgcttttca atataaggaa gttctacatc
     1141 tgaaatagta ccatcatcat ttgaagaaat atgtgttaaa ggggctaaca caaatcgatt
     1201 tctcaactct actccatttg gtaattctac tttatcaaac aatggttcgt atttactttt
     1261 cattacttaa ttcctcctta aaattattga gttgactata acatgtaatt ttttcattct
     1321 ccaattatct gtttatatag ttgcaattcg tttaatactt taagttgtag ttctcatttc
     1381 taaaataaat taaaaaaaca aatccacata caatattgca aatcaatgat tctatctatc
     1441 aaagataatg gatatatcac cttaaaccat ttgtacgtgt aaatagaatc aatcatatta
     1501 cgttatcgta gacaaaaaga gattagtatc taataaaatt taatagatac taatctcttt
     1561 tatagtctaa gaaactttca tttcagaccc tttgtatgat tttcggtacg atttatctgt
     1621 tcctttagtc gtttcgaatt gctcgctttg actgttttat ccagattggt aagcgttctt
     1681 taagtgtttg aaacccatat ttttctgttt ctctgatttc atctaatact gattgttttt
     1741 ccttcttacg ctcataattt ttgaagtaat cattatcctt taatgataga ttaagctttc
     1801 cttcatcatc tatagacaaa attttagctt taacaatttg gccttctgat agaaatttct
     1861 tcaaattatg aacgtagtca tccataattt ctgatatatg aatcagtcct tcagtatgat
     1921 taggggtctc aacaaacgca ccgtatggtt gaataccagt tacacgcacc ttgatatgtt
     1981 ggccaatttt gtagttattc aacttaataa ccccttattt cgtttactta acactattat
     2041 catagcacaa atcgaaatgg tggactaatt aaaacatcaa atcatacgaa tattatttta
     2101 taaatgaata agaattctac aaacttggat ttacaaataa aattagcaac aaactcctat
     2161 ctaaatcaat atgatatcca gctactttgt tttcaattta ttaactattt gaatagcaat
     2221 tctctttaaa aacataaaaa gcaacctcaa ctattatctt tagaatacac atctatttaa
     2281 aaactgtgct ttcttgataa atatattgag attgctcatc atgttagttt atttgtttcg
     2341 ttggaatata tactaatcca ttcataaatt agctttatcc gagtttaaaa tgttggtagt
     2401 taattatgtt tagatattat tcttcaacat caatagattc aattacaaca tcatgaagtg
     2461 gtttgtcttg tggtcccact tttgtatttg caatatcttc taatgtagtt tcaccatcaa
     2521 tgatttgacc gaatactgta tgtttttgat ctaaccatgg tgtaccaccc ttctcgccat
     2581 atgcatcaac gattggttgt ggccaaccac catctgcaag ttgacttaac atattttgag
     2641 gtacttcttt catttgaaca atgaaaaatt gtgaaccatt agtattaggt cctgagttag
     2701 ccattgataa tgcgccatat aagttaaatg catttaatga aaattcatct tcaaaagcac
     2761 cgccataaat actttcgcca cccataccag tagctgttgg atcgccacct tgaatcatga
     2821 agtcattaat gacacggtgg aatgttattc catcataata accatttttt gcatgtgtca
     2881 caaaattttc aactgttttt ggtgcaatat ttggaaataa tttgaatgtc atgtcacctt
     2941 tatttgtgtg cataaccact ttaatttcac cttgttgtac ttctttgttt aactgtggat
     3001 agttagccat ttatattctc cattcatgtt atgatacata tatattaaca tacttttaag
     3061 ggaggaaaat agcatgaagc tatatcgatt ttcacacaaa acaggtagct atggtgtcac
     3121 gattgttgaa gaaaatggag acgatgtcct tgttaaagtt gaacaagtta tcaaacaccc
     3181 gaaacaaggt gatttacata accctactga aactgaaggt gtatttttcc atgaacgcaa
     3241 agcattaagc cattttgaaa aacgctatac gaaacgttca caattacgcg acttcaatgt
     3301 tgatgtattg cgctatgaag attcacttca acaagcaatt acagatttag aaactaaact
     3361 taaagctcag caaacaaaac atgctgaaat gtctttagct agtttagcac gcttaaaaga
     3421 agattattca cttcaataca aagaaaattt taaataaaaa atgacgtgtt tcttcttatt
     3481 taaatacttc tgaaaataaa gcacaacaag gagtgtgtac gtttttcaga actatatcat
     3541 tcatgtata
//
LOCUS       DAHHYJ010000184         3516 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000184 DAHHYJ010000000
VERSION     DAHHYJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.188"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..2066
                     /locus_tag="NBK22_002462"
     CDS             <1..2066
                     /locus_tag="NBK22_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCD2415617.1"
                     /translation="LKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQIAQG
                     SHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEMLDTA
                     EKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDKQLLS
                     KIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSKLPGV
                     NTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYLEYQY
                     EDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDKQIKK
                     LRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGSSVKG
                     GTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSNVYMF
                     KTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETRGQIEPLTN
                     NPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGPLKKK
                     INGTVLNKVNNTEKEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEVFQNG
                     EPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGKDDKN
                     KDKDK"
     gene            2179..2328
                     /gene="rpmG"
                     /locus_tag="NBK22_002463"
     CDS             2179..2328
                     /gene="rpmG"
                     /locus_tag="NBK22_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD2415618.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            2537..3076
                     /locus_tag="NBK22_002464"
     CDS             2537..3076
                     /locus_tag="NBK22_002464"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCD2415619.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGIY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            3088..>3516
                     /locus_tag="NBK22_002465"
     CDS             3088..>3516
                     /locus_tag="NBK22_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD2415620.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKV"
BASE COUNT         1399 a          478 c          629 g         1010 t
ORIGIN      
        1 gactaaaaga aaaatcaaat gatgaaatcg ttcaaaatac aattaacaag agaattaact
       61 ttatatttgg tgtaattgta tttatttttg cagtactagt actacgttta ggttatttac
      121 aaatcgcaca aggctcacat tataaacaaa ttataaaaaa tgatgaaaac attacagtga
      181 atgagtctgt gccaagaggt cgtattttag acagaaatgg gaaagtttta gttgataatg
      241 cttctaaaat ggctattaca tatactaggg gtcgaaaaac aacacaatcg gaaatgttgg
      301 atacggctga aaagttatca aagctaatca agatggatac taagaaaatt acagaacgtg
      361 ataagaaaga tttctggatt cagttgcatc ctaaaaaagc aaaagcaatg atgacaaaag
      421 aacaagctat gttagcagat ggaagtatta aacaagatca atatgataaa caactgttat
      481 cgaaaatcgg aaaatcacaa ttagatgaat tgtcttctaa agatttacaa gttttagcta
      541 tttttcgaga gatgaatgca ggaacagttt tagatccaca aatgataaaa aatgaagatg
      601 tcagtgaaaa agagtatgca gcagtttctc agcaactttc caaattacca ggtgttaaca
      661 cgtctatgga ttgggataga aaatatccat atggcgatac tttaagaggt atattcggag
      721 atgtatcgac acctgctgaa ggtattccaa aagaattgac agaacattac ttatccaaag
      781 gatattcacg caatgatcgt gttggaaaat cttacctaga atatcaatat gaagatgtat
      841 tgcgtggtaa gaagaaagaa atgaaataca caacggacaa atctggtaaa gttacatctt
      901 cagaagtgtt aaatcctggc gctcgcggtc aagatttgaa attaacgatc gatatagatc
      961 ttcaaaaaga agtagaagca ttattagata aacaaattaa gaagcttcgc agtcaaggtg
     1021 ccaaagatat ggataatgca atgatggttg tacaaaatcc taaaaatgga gacattcttg
     1081 cgcttgccgg aaagcagatt aataagagtg gtaaaatgac tgattatgac attggtacgt
     1141 ttacttctca atttgcggtt ggatcttctg taaaaggtgg aacattatta gctggttatc
     1201 agaataaagc tatcaaagtt ggagaaacaa tggtcgatga accattacat ttccaaggtg
     1261 gtttgacaaa acgatcatac ttcaataaaa acgggcatgt atctattaat gataagcaag
     1321 ctttgatgca ttcatcaaac gtatatatgt ttaaaacagc attaaaatta gcgggagacc
     1381 cttattattc tggtatggct ttaccttcag acataagttc acctgcccaa aagctaagaa
     1441 gaggattaaa tcaagtaggc ttaggtgtga aaacagggat agatttacca aatgaaacaa
     1501 gaggtcaaat cgaaccatta acaaataatc caggtaatta tctagattta tcaattggtc
     1561 aatatgatac ctatacacca ttacaattat cacaatatgt ttcaactata gcgaatgatg
     1621 gttatagaat acagccacac attggattaa cgattcatga atcaactaat aaagatgagg
     1681 ttggtccact caagaagaaa attaatggca ctgtcttgaa caaggttaat aatactgaaa
     1741 aggaaatcaa acaaattcaa gaaggattca aaatggcatt taatgataaa gatggtactg
     1801 gatatgttag ttttaaagat acagtagtac ctactgctgg taaaacgggt accgctgaag
     1861 tgttccaaaa cggagagcca agagttaact ctacttatat aggatacgcg ccaattgatg
     1921 atccaaaatt agcgttttca attgtatata caaatcagcc tgtaccacca ccatggttaa
     1981 caggtggaga cttaggtaga gatgtaatta actactactt taagcagtta ggtaaagatg
     2041 ataaaaataa agacaaagac aaataaaatt taacctgacg attgtgtagc gcatggttgt
     2101 aaaattttaa ctttgcaaat attatagatg ttggtataat aataaagtcg tatttagaaa
     2161 tgataaggag gaataaccat gcgcgtaaac gtaactttag cttgtacgga atgtggtgac
     2221 agaaactaca ttacaactaa gaacaaaaga aataatccag aacgtgttga aatgaagaaa
     2281 ttctgttcac gtgaaaacaa acaaacttta caccgtgaaa caaaataatc attaaaatta
     2341 aatttaaatt aacaataaaa aaggtgaaat ctcaaaataa gaggtttcac ctttttaatt
     2401 atgcacacgt atgaaatgat aaaagatttt caattgaaat aataaatttt agaattgtcg
     2461 cataattcat catgacaaca taatgacatg tattgttatt aacgatataa tagaattgaa
     2521 taaagtgggt gataatgtga ctaaaaatga gattagaaaa tacattttac ataaaatgaa
     2581 gaattttaat aaagctgaaa agcgaaaagc agacacatgg ttaagaaatc aattttttgc
     2641 aactgaagaa tacaaagaag caaacgcaat tgcgctagtt ctttctttta atcatgaagt
     2701 agatactttt tctattattg aacaagcctt aatggatcat aaacgtattt ttgtaccgaa
     2761 aatggattat ttaaatcatc aaatgacttt taaagaaata tttaatctca aagatattga
     2821 tgtcgataat aaggggattt actatccaac ttcaaaaggt gaaacaacga ataacctaga
     2881 tttaattgtt gttcctggtg ttggatttca agacgatgga tatagaattg ggtatggtgg
     2941 tggctattac gacaggtttt tagctaatta tcagacaaag acaataagct tattatacga
     3001 ttttcaaata acatcatttg aaccggaatc attcgatcaa ccagtcgata aattgattat
     3061 atatcaatca gcatagtgga ggactaaatg aacatagaca aacaattttg gaaaacaata
     3121 tattattgga ttaggtactt gaattttgat attgtcagta gggaaaaaga tgaccaagaa
     3181 atttggttag cccataaaag aaaaaaacaa gtagtcattt ttaaacaaca tatcaagtct
     3241 acccaagaaa tacgttttga caaagcgaaa gtgcttgaac acaaagatga aatagcaaat
     3301 tttatttctt tcgaaccaca aagttttgaa ttttattatt ttacagaatc ggaattttca
     3361 gaagaacaat taaatgaagt ttcgccaatt agaattaaat tcaatgttat aagacacaca
     3421 aaagatttga taaagcatat gccgaatata tttttggcta gacttatttc agaagataat
     3481 gataaaaaga catatatgtt ctataaacgc aaagta
//
LOCUS       DAHHYJ010000185         3470 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000185 DAHHYJ010000000
VERSION     DAHHYJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.189"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            213..1337
                     /locus_tag="NBK22_002466"
     CDS             213..1337
                     /locus_tag="NBK22_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCD2415621.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSIMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDAIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNTYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(1418..2371)
                     /locus_tag="NBK22_002467"
     CDS             complement(1418..2371)
                     /locus_tag="NBK22_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCD2415622.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKN
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(2733..3233)
                     /locus_tag="NBK22_002468"
     CDS             complement(2733..3233)
                     /locus_tag="NBK22_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD2415623.1"
                     /translation="MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
BASE COUNT         1181 a          542 c          611 g         1136 t
ORIGIN      
        1 ctaggacatt gatttatgtc ccaagctccc acatcattgt tacaaatgta catgatgtaa
       61 atgaatattg ctgtctaaat gtgcatgtaa tgtacaatgg tgcagataat acacttaaat
      121 ctttaaaaat gaaacgttag ttccaagagt catttttaaa caatagtgca tgtgataaaa
      181 tagaaaagaa tgaaaaatat agaggtgaca atatgaagat agcaattata ggtgcaggca
      241 tcggtggatt aacagctgct gcattattac aagaacaagg tcatactatt aaagtctttg
      301 aaaaaaatga gtcagttaaa gaaattggcg ctgggattgg tatcggagat aatgtgctta
      361 aaaaactagg taatcatgac ttagctaaag gtattaaaaa tgctgggcaa atcttatcta
      421 taatgacagt gttagatgac aaagatcgcc tgttaactac tgttaaatta aaaagtaata
      481 cattgaatgt gacgttacca cgccaaacat taattgacat tattaaatct tatgtaaaag
      541 atgacgcaat atttacaaat catgaagtca cgcatataga taatgagaca gataaagtta
      601 ccatacattt cgcggaacaa gaaagtgaag catttgattt atgtattggt gctgatggaa
      661 ttcattctaa agtgagacaa tctgtaaatg ctgacagtaa agtattatat caagggtata
      721 catgctttag aggtttaatt gatgatattg atttaaagca tccggattgt gcaaaagaat
      781 actggggaag aaaaggaaga gtaggtattg ttccgttatt aaataatcaa gcatattggt
      841 tcattacaat taactcgaag gaaaacaatc ataaatatag ttcgtttggt aaacctcatt
      901 tgcaagcata ctttaatcac tatccaaatg aagttagaga gatcttagac aaacaaagtg
      961 aaacaggtat cttattgcat aatatttatg atttgaaacc actcaaatct tttgtttatg
     1021 gtcgtactat tttactagga gatgcagcac atgcgacaac gcctaatatg gggcaaggtg
     1081 ctggacaagc aatggaagat gctatcgtat tagtaaattg ttttaacaca tacgactttg
     1141 aaaaagcatt acagcgttat gataaaatac gtgtcaaaca tactgcaaaa gtaattaagc
     1201 gttctagaaa aatcggtaaa attgcccaat atcgtagtcg tttagttgtt gcagttagaa
     1261 atcgtattat gaaaatgatg ccaaatgcat tagcagctgg acaaactaaa ttcttatata
     1321 aatcgaaaga gaaataatac aacaatatga aaacccccgt atgttgaaac gagagctcaa
     1381 catatggggg ttcttgtttt tataataaat tcaattatta gttaacgaca aattgtggtt
     1441 tctcaccttg aacggcacta attgcagcat tagcaacaat tttagacatc atgtcacgtg
     1501 cttcaaatgt agcattacca atatgcggtg ttaatactac attattaagt gattttaagt
     1561 catcggtaat atctggttca aattcatata catcaagtgc agcaccttca atttcattat
     1621 ctttcaatgc ttgcactagt gcttgttcgt gcacgattgg accacgagag gcattgatta
     1681 aatacgccgt agatttcatc attttaaatt gttctgtatc aattaaatga tgcattttag
     1741 gattataagc agcgttgata gtgataaaat ctgcattctt taatagtgta tctaaatcta
     1801 catattttgc accgatttct cgttcttttt cttctttgcg attaggtcca gtgtatagca
     1861 catccatgtc aaatgctctt gcacgacgag ctactgcact accaatttca cctaaaccga
     1921 taatgccgat tgttttccca gatacttctc tacctctgaa aaataaaggt gcccatccgt
     1981 caaatccagt tgtacgtgat aattggtccc cttcaacaat acgacgcgct actgcaagta
     2041 ctaatccaat tgttaaatca gcagtcgcgt ttgttgatgc tttaggtgtg tttgtaacat
     2101 ctatactttt ttctcgggca tactcgatat caatattatt aaaaccagcg ccatagttgg
     2161 caatgatttt taagtcttta ccagcatcga taacatcttt atcaacgttt gtagataata
     2221 aactaattaa ggcagtcgcg tttttaacac ctttaattaa agtgtcttta tcgactaatc
     2281 ctttaccttc atacatttca acttcaaaat gttcttgtaa aagttttaaa cctacttctg
     2341 gtattgcacc agcaacataa actttttcca taaaagatca ctccttttat cttagtatag
     2401 tagaagatta gacagtatac aactatgtca tgatgtcttg tgtatcaatg atgtaagcgc
     2461 gtacttttga tggaggtgat ataacttagg cactgtagaa ctatgaatat tgtaatgtgg
     2521 aaaaactgga tcaattaaat tagataacgt agttttaaag ttaatagtat tagaaaaaat
     2581 taatattatg aatatgggag gaaatataaa taagtaggtg gcaacgaaaa atagcaaaaa
     2641 aagagcttct cctataaagg aaagctcaaa gttttttgat gacatatgta ctagaattaa
     2701 gtttcaagac aatatgtatc atcgtgttta tattaaatat ggatgtagtt gtagttacct
     2761 gcttcacttg cagaaatagt tctagaactt actgagaaag gtccgccact ataattcatt
     2821 tctgaaattg taactgaacc atcactgttt acactttcta catatgcaac gtgaccaaat
     2881 ggcccttcag aagattgtag gatagcgcct ttagaaggtg tatgatttac tgtgaatcca
     2941 gcaccttgtg cagcagcagc ccaattatta gcatttcccc aagtagaacc gatttctccg
     3001 ccaactttat catatacata ccaagtacat tgtcctgcag tgtataagtt accagaatgt
     3061 gaaattgatg atgtagttgt cgtagttgtt gtagtcgttg tagtttgagt cgtgttgtag
     3121 ttatagttgt tgtaatttgt ataattttca gcagcatctg catgatgtgc ttgacctact
     3181 aatgctgtgc cgattcctgc tgttaacgta gttgctgtta ctaatttttt catgaataaa
     3241 gtcctccaaa gttctatatc tttttttata aataaaacgt agcgactgtt ttattctcac
     3301 atctcgaatt gatgacaata gttactttaa caaaattaat gcttcttgtg gggaatgtta
     3361 ttgatttgta aaagaataaa aaaactttga ctaattttgt aataaaaatt agtcaaagtt
     3421 acaatgagat taacagataa ttaataggaa atatttattt gtaatatgtt
//
LOCUS       DAHHYJ010000186         3377 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000186 DAHHYJ010000000
VERSION     DAHHYJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.190"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(165..1067)
                     /gene="cvfB"
                     /locus_tag="NBK22_002469"
     CDS             complement(165..1067)
                     /gene="cvfB"
                     /locus_tag="NBK22_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCD2415624.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQPGDYLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKIALTKKGWSRMDSKE"
     gene            1214..2815
                     /locus_tag="NBK22_002470"
     CDS             1214..2815
                     /locus_tag="NBK22_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2415625.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNISFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSYKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
BASE COUNT         1127 a          562 c          560 g         1128 t
ORIGIN      
        1 taatagaaat cagctttttt acattgccta agaacttaat gtcccagcct attcgtttga
       61 attatttaat tttttacttt gaaaggaatg tttaaaaaac tacttcatct gtccgattta
      121 atattgaaaa aaaaacacgc atcctacgac acgtgtaaaa atgattattc ttttgagtcc
      181 attcgactcc aacctttttt agttaaagcg attttacctg tttctatatt aataatcttc
      241 tgtttatata agtgaccgat tgcacgtttg aatgaacctt tactcatatt gaatacttct
      301 ttaatcgctt cagggcttga tttgtcccag aatggtaatt caccatcata ttcaactagt
      361 aaatcaaaga tgacttggcc gtcatcgtct aaacgttcat gtgcaagtgg taaaaatgaa
      421 ccatttaact cacctttatc attatgcccg ataattctaa cttgaacaga ttcacctaat
      481 cttggttcag ctttacgttc tgattcatgt acgaaaattt tgtaacctga ttcgcttaat
      541 aaaaagctac caattcgtaa tacgcggtaa ggtttggctt caatgacttc gttttttaaa
      601 ttatcgtcgt gtacaggtgt aaacatattt tctacaacag attcactcgc taaacgtcca
      661 tacatatgat tctcacggtc aattcgtaat gtaactagca aataatcacc aggttgtggc
      721 catagtgatt tcacttttgg taaatcttcc catggtacta acacttcacg gggtaatcca
      781 acatctatac gtgccccatc gcgatccgtt ttaagtactt tagcaaagtc atatttatct
      841 ttcgtaatat caggcatatt ttgagttgca aataattcac ctgaacggtt tggataaatg
      901 aagaaactat attcttcacc tacttctaat tcatcatcat cgttcatttc tgattggttt
      961 aactttacgt tttcaccgtt tggtcctttt aaaaggtaag ttgaaccttg taaccctact
     1021 acttcaagga attctataga acctactata tctttgtcta atgccatgtt attctccttc
     1081 gattcttgta ttctttatta ttataacatg tgtgagtcat tctatcatta caacttatat
     1141 gaggtcgaaa ctgtgacgat aatatgatat aattcttccg ttagaatatc gaatgaaaaa
     1201 aaggagaaaa tgcatgttac aagtaactga tgtgagttta cgttttggag atcgtaaact
     1261 atttgaagat gtaaatatta aatttacaga aggtaattgt tatggattaa ttggtgcgaa
     1321 tggtgcaggt aaatcaacat tcttaaaaat attatctggt gaattagatt ctcaaacagg
     1381 acatgtttca ttagggaaaa atgaacgtct agctgtttta aaacaggacc actatgctta
     1441 tgaagatgaa cgcgtgcttg atgttgtaat taaaggtcac gaacgtcttt atgaggttat
     1501 gaaagaaaaa gatgaaatct atatgaagcc agatttcagt gatgaagatg gtatccgtgc
     1561 tgctgaactt gaaggtgaat ttgcagaaat gaatggttgg aatgctgaag ctgatgctgc
     1621 taacctttta tctggtttag gtatcgatcc aactttacac gataaaaaaa tggctgaatt
     1681 agaaaacaac caaaaaatta aagtattatt agcgcaaagt ttattcggtg aaccagacgt
     1741 actattactg gatgagccta ctaacggtct cgatattcca gcaatcagtt ggttagaaga
     1801 tttcttaatt aactttgata atactgttat cgtagtatcg catgaccgtc atttcttaaa
     1861 taatgtatgt actcatatcg ctgatttaga cttcggtaaa attaaagttt atgttggtaa
     1921 ctatgatttt tggtatcaat ctagtcagtt agctcaaaag atggctcaag aacaaaacaa
     1981 gaaaaaagaa gaaaaaatga aagagttaca ggactttatt gctcgtttct cagctaacgc
     2041 ttctaaatct aaacaagcaa caagtcgtaa aaaacaactc gagaaaattg aattagatga
     2101 tattcaacca tcatcaagaa gatatccttt cgttaaattc acacctgagc gcgaaatcgg
     2161 taatgactta ctaatcgttc aaaatctatc taaaacgatt gacggtgaaa aagtattaga
     2221 taatatttca ttcacaatga atccaaatga taaagcaatt ttaattgggg atagtgaaat
     2281 tgcgaaaacc acattgctta aaatattagc cggtgaaatg gaaccagacg aaggttcata
     2341 taaatggggt gtaacaacgt cattaagtta cttccctaaa gataactcag aattctttga
     2401 gggcgttaat atgaaccttg tagattggtt aagacaatac gcaccagaag atgagcaaac
     2461 tgaaacattt ttacgcggct tcttaggccg tatgctattt agtggagaag aagttaagaa
     2521 aaaagctagt gtactttcag gtggagaaaa agtacgttgt atgctaagta aaatgatgtt
     2581 atcaagtgca aacgttcttt tacttgatga accaacaaac cacttagact tagaaagtat
     2641 tactgctgtt aatgatggac ttaaatcatt caaaggttct attatcttta cttcatatga
     2701 ctttgaattt atcaatacaa tcgcaaaccg tgttatcgat ttaaataaac aaggtggcgt
     2761 ttcaaaagaa attccatatg aagaatattt acaagaaatc ggcgttttaa aataaacata
     2821 atatgtagaa tttcttttcg aaaatctctg tattgaggcc cgaccaactt gcacattatt
     2881 gtaagttggc ttactgtcac cttctatatt gggacttatt ccgcaacttg tattgtttgt
     2941 agaaattagg aatccaattt ctctatgtgt tgcaccgaca aggattgaaa aaagctagct
     3001 acaaagtcat tttcattcag tcaactactg tcaatataac attgcagggc ctagggcatt
     3061 gattcatgtc cgagactata ttcaatttaa tagacattaa ctgggcgtct cgatgctaat
     3121 atttatagca tcgaggtgtc ctttttattt tattttttaa cacatcagca tagtgaacac
     3181 cccttctcta aattacaaat acagttatca acgcattaaa agagtacatt tatttaatgt
     3241 gcatattgat tatgctataa gtattttgaa ttttattttt caaaaagtct cattatcacc
     3301 accttttaaa atataagcaa cgctataact tgaattttca ggttattcca aaaaatctaa
     3361 attatcggtt tacattc
//
LOCUS       DAHHYJ010000187         3335 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000187 DAHHYJ010000000
VERSION     DAHHYJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.191"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            227..1021
                     /locus_tag="NBK22_002471"
     CDS             227..1021
                     /locus_tag="NBK22_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2415626.1"
                     /translation="MIHSKKLTLGICLVLLIILIGGCIIMTKINSRNAQIKDIFNQTL
                     NVYPTKNLDDFYDKEGFRDQEFDKRDKGTWIINSGMYIQLKGGALKSRVMVLYINRNT
                     RTAKGYFLISETTEDKKGYVHNKDKKYPVKMERNRIIPTKPITDEKLKKEIENFKFFV
                     QYGNFKDFKDYKDGDISYNPNVPSYSAKYQLNNDDYNVQQLRKRYDISTKRAPELKLR
                     GSGDLKGSSVGSKELEFNFVRNKEENVYFSDGINFKPTEEMNHEQN"
     gene            1008..2333
                     /locus_tag="NBK22_002472"
     CDS             1008..2333
                     /locus_tag="NBK22_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415627.1"
                     /translation="MNKTKYQINSDNIKSNSEETSAISSISYEIENANNNDLNNASIQ
                     TQIEILKSQNSFPKNLSYLKSYTDPKTGTTTSAFLNKDTGKVTLGMTGTNVHKDAILK
                     QTFGVPSYQGYIDASETLKDIGADVNIGLHSVTDKDPHYKNTQDFIKNIKKDYDIDII
                     TGHSLGGRDAMILGMSNDIKHIVVYNPAPLAIKDVSGLYADQEELKKLIEKYDGHIVR
                     FVSDEDELDAGVRNHLYETAGEKIVLKNGEGHAMSGILMSRTQAIILAELNKVKGYQD
                     ENNKALKSVRKQTRHRLHKVETLRANWIQTTGGSLSSSQQQLLEALTALTIAEGLNQL
                     VNEESQHLKKMYHAMAHKFGDNWKKAQEVGNEIGEKLTSEEVIDELRKGGAYESKLET
                     DPKRKIDDKIKKLNDVYKNCNGYIAKIKQSIEAIVSNDQMLASQIDGMM"
     gene            2333..2659
                     /locus_tag="NBK22_002473"
     CDS             2333..2659
                     /locus_tag="NBK22_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415628.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            2662..2949
                     /locus_tag="NBK22_002474"
     CDS             2662..2949
                     /locus_tag="NBK22_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415629.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKNFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
BASE COUNT         1372 a          430 c          585 g          948 t
ORIGIN      
        1 aatgaaaatt ccataaagca ctaggtaaaa gattataacg ataactctta aaattaataa
       61 taaatcacag aaactttatc ttttgattta attgttttat aattctgata agaagattta
      121 aaatacaaaa taattacaat taaattcatt tgttcaaaac aatttttcgt tttagttgta
      181 aatattaaaa actgagtcag aattataaat ataaggtgga gaaatgatga ttcattcaaa
      241 aaaattaacg ctcggtatat gtttggtatt actcattata ttgattggag gttgtatcat
      301 tatgacaaaa ataaatagtc gaaacgccca aattaaagac atatttaatc aaacattaaa
      361 tgtgtatcca accaagaatc ttgatgattt ttacgataag gaaggctttc gagatcaaga
      421 atttgataaa agagataaag ggacttggat tattaattct ggaatgtaca tccaattaaa
      481 aggaggagcc ttaaaatcca gagtgatggt actttacatc aatcgtaaca ctagaacagc
      541 taagggatat tttttaataa gtgagacaac agaagacaaa aaaggatacg ttcataataa
      601 agataaaaaa taccctgtca agatggaacg taatcgaata attccaacga agcccataac
      661 ggacgagaag ttaaaaaaag agattgaaaa ctttaagttc tttgtacaat atgggaattt
      721 taaagatttt aaagattata aagatgggga tatttcatac aatcctaacg taccaagtta
      781 ttccgcaaag tatcaattga ataatgatga ttataatgtc caacaattaa gaaaacgata
      841 tgatatatca accaaaaggg cacctgaatt aaaattgaga ggatctggcg atttaaaagg
      901 ctcatccgta ggttctaaag aactagaatt taactttgta agaaataaag aggaaaatgt
      961 gtatttttct gatggtatta atttcaaacc tactgaggaa atgaatcatg aacaaaacta
     1021 aatatcaaat aaattctgac aatataaaga gtaactctga agaaacaagc gcaatatcta
     1081 gtataagtta tgaaatagaa aacgcaaata ataacgattt aaataatgca agtattcaaa
     1141 cgcaaataga aatattaaaa tcacaaaata gtttccctaa aaatctttca tatcttaaaa
     1201 gttatacgga ccccaaaaca ggtacgacta caagcgcctt tttaaataaa gacactggca
     1261 aagttacttt aggtatgaca ggtactaatg tacacaaaga cgcaatatta aaacaaacat
     1321 ttggtgttcc ttcttatcaa ggatatatag atgcgagtga aacgctaaag gatattgggg
     1381 ccgatgtcaa tattggcctt cattccgtca cagataaaga tccacattat aaaaataccc
     1441 aagactttat caaaaatatc aaaaaagact atgatattga tattattacc ggacattcgc
     1501 tgggcggtag agatgcgatg attttaggta tgagtaatga tattaaacat atcgttgtgt
     1561 ataatccagc accattagcg atcaaagatg tgagcggcct ttatgccgat caagaagaac
     1621 ttaaaaaatt gattgaaaag tacgatggtc acattgtaag atttgtgtct gatgaagacg
     1681 aattagatgc aggtgtccgc aatcatttat atgaaactgc tggagaaaaa atagtactta
     1741 aaaatggaga aggccatgca atgagtggta ttttaatgag cagaacacag gctataatct
     1801 tagctgaatt aaacaaagtt aaaggctacc aagacgaaaa taataaagca ttaaaatccg
     1861 ttcgtaaaca aacgaggcat agattacata aagtagagac gttaagagcg aattggattc
     1921 aaacaacggg tggatcactc tcttcctccc aacaacaatt attagaagct ttaacagcac
     1981 taaccattgc cgaaggctta aatcaattag tgaatgaaga aagccaacat ttgaaaaaaa
     2041 tgtatcacgc gatggcacat aaatttggag acaactggaa aaaagcgcaa gaagttggaa
     2101 atgaaattgg tgaaaaatta acctctgaag aggttataga tgaattaaga aaaggtggcg
     2161 cgtatgaaag taaacttgaa acagatccca aaagaaaaat tgatgataag ataaagaaat
     2221 taaatgatgt ttataaaaat tgtaatggct atatcgcaaa aattaaacag agtatcgaag
     2281 caattgtttc taatgaccaa atgttagcga gccagattga tgggatgatg taatgtttac
     2341 tacgtataaa aatattaatg aacttgaaaa tgcctatgat gaagaaagaa aacaattgaa
     2401 tgatgcattc aatcaaattg atgaattaag acatcaaaca cgcaagaaat gtgaacaaat
     2461 gtatgatcat ttcttatatc tcaaacataa aatgaattat agtgaagacg ctatgatcag
     2521 gatgacacgt attatagaat ctttcgatag agaaacgaat caacgtatcc gacatcacga
     2581 aatgaaatta gaagattata aagatgagtt aagaagagaa tatctaaaac aatctgacag
     2641 aattgaagga gatgaataag cgtgggactt gattttagtg gtttaccaga tttagcagta
     2701 ttggaacaaa tgaaggaaaa agaacagatt agtgaggtta ttgcgcctga acatgttcgt
     2761 atgcatcatg atcatcaaaa taagctgaaa agtgatgaga aaatattact tgaccaaatg
     2821 gtaagtcatt tcaaaaactt tgaagatgat tttaaaaatg cggcacaagg ggcttgggtg
     2881 aaaaatgcca cagacgaatt aaaagatatt agtaatgatt tagaaaaaat tcaagatatt
     2941 aaagtataaa aaggtattaa gaaacacaat aactatataa tccatttaat aataaataaa
     3001 ttatatagtt cataatttcg actataagtg gctattagcc attatttttc gggatctttg
     3061 tcaaatcgga ctaatgaatt caataatgga agttaagcaa ccaatctttg tttaacttct
     3121 tttttatttt ttggaaaata aagttttgaa cataataaaa tttgattatg ttttaacgaa
     3181 ttttgatgtt tcttgaacta tatatcatct agtcgtcatt tacaattggt aaatatgact
     3241 tcaaactgta tgaaggtgat ggcgattaaa aggctcatcc gtaggttcta aagaactaga
     3301 atttaacttt ataagaaata aagaagaaaa tgtgt
//
LOCUS       DAHHYJ010000188         3325 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000188 DAHHYJ010000000
VERSION     DAHHYJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.192"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..356
                     /locus_tag="NBK22_002475"
     CDS             <1..356
                     /locus_tag="NBK22_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD2415630.1"
                     /translation="DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLFGGLFAA
                     LGSLLLFGRRKKQNK"
     gene            750..>3325
                     /locus_tag="NBK22_002476"
     CDS             750..>3325
                     /locus_tag="NBK22_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046462978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HCD2415631.1"
                     /translation="MINRDNKKAITKKDMISNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQP
                     EAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNN
                     TNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKE
                     ELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRVNAKMRFAVAQPAAVASNNVN
                     DLIKVTKQTIKVGDGKDNVVSAHDGEEIEYDSEFTIDNKVKAGDTMTVDYDKNVVPSD
                     LTDKDLPVDITDSSGEVIAKGAYDRATKRITYTFTDYVDKYENVKSRLTLYSYIDKQA
                     VPNKKAVNLTFSTAGSSLSKDVTVDYQDPMVQNNSNIQSIFTKLDEDKHTIEQTIYVN
                     PQQKNAKDTRVDIAGNEVDSNGNFKYGNGGTIIDSNSEIKVYKVKKGQQIPDSNRIYD
                     YSQYEDVTSQFDDKKTFTNNQVRLNFGDIDSTYIVKVVSKYTPTSDGDLKIIQGTRMI
                     TTNDYYNTDDYAGYSNFIVSSTDTGGGDGTVKPEEKLYKIGDYVWEDVDKDGVQGTDS
                     KEKPMANVLVTLTYPDGTTKSVRTDANGHYEFGGLKDGETYTVKFETPTGYLPTKVNG
                     TTDGEKDSNGSSVTVKINGKDDMSLDTGFYKEPKYNLGDYVWEDTNKDGIQDANEPGI
                     KDVKVTLKDSTGKVIGTTTTDASGKYKFTDLDNGNYTVEFETPAGYTPTVKNTTADDK
                     DSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQDSTEKGIKDVTVT
                     LQ"
BASE COUNT         1368 a          497 c          574 g          886 t
ORIGIN      
        1 cagactcaga tagcgactca gactcagata gcgactcaga ctcggatagc gattcagatt
       61 cagacagtga ttcagattca gacagcgact cagattcgga cagtgactca gactcagata
      121 gcgactcaga ctcggatagt gactcagact cagatagcga ttcagactcg gatgcaggaa
      181 aacatacacc tgttaaacca atgagtacta ctaaagacca tcacaataaa gcaaaagcat
      241 taccagaaac aggtagtgaa aataacggct caaataacgc aacgttattt ggcggattat
      301 tcgcagcatt aggatcatta ttgttattcg gtcgtcgtaa aaaacaaaat aaataataca
      361 gcttatacca ggtccgtgag gcctggtttt ttatttcaaa atttatgaaa tgtatcttat
      421 gtttgagatt aaatagtgca atttttaaaa aaatataaca taaatcaaca taaatttaat
      481 tcatctttac attaaaagta caaacgaata agaaaagcaa ttaaacacag tatcaaacca
      541 tttaaaacaa ttgatataaa aaacaaacga ataattaaat attactagaa ttaatttatt
      601 caaaccaaag tgtaaaataa cagtcttaga taaataaatt tatttaaagt attgtgcttt
      661 atctaaaaat gtattacgat gggaatacaa atttttatat gtaaaaatat taattttttg
      721 caaatattga tattttaaag gagatttata tgattaacag ggataataaa aaggcaataa
      781 caaaaaagga tatgatttca aatcgcttaa acaaattttc gattagaaag tatactgtag
      841 gaactgcatc gattttagta ggtacgacat tgatttttgg tctagggaac caagaagcta
      901 aagctgctga aagcactagt acagaaaatg caaaacaaga tgatgcaacg actagtgata
      961 ataaagaagt agtgtcggaa actgaaaata attcgacaac agaaaatgat tcaacaaatc
     1021 caattaagaa agaaacaaat actgattcac aaccagaagc taaagaagaa tcaactacat
     1081 caagtactca acaacagcaa aataacgtta cagctacaac tgaaactaag cctcaaaaca
     1141 ttgaaaaaga aaatgttaaa ccttcaactg ataaaactgc gacagaagat acatctgtta
     1201 ttttagaaga gaagaaagca ccaaataata caaataacga tgtaactaca aaaccatcta
     1261 caagtgaaat tcaaacaaaa ccaactacac ctcaagaatc tacaaatatt gaaaattcac
     1321 aaccgcaacc aacgccttca aaagtagaca atcaagttac agatgcaact aatccaaaag
     1381 aaccagtaaa tgtgtcaaaa gaagaactta aaaataatcc tgagaaatta aaagaattag
     1441 ttagaaatga taacaataca gatcgttcaa ctaaaccagt tgctacagct ccaacaagtg
     1501 ttgcaccaaa acgtgtaaac gcaaaaatgc gctttgcagt tgcacaacca gcagcagttg
     1561 cttcaaacaa tgttaatgat ttaattaaag tgacgaagca aacaataaaa gttggtgatg
     1621 gtaaagataa tgtagtttct gcacatgatg gtgaagaaat tgaatatgat tcagaattca
     1681 caattgataa taaggtcaaa gccggcgata ctatgactgt tgattatgat aaaaatgttg
     1741 tgccatctga tttgacagat aaggatcttc ctgtagatat tactgattca tctggtgaag
     1801 ttattgcaaa aggtgcatat gatagagcaa ctaaacgtat tacttataca tttacagact
     1861 atgtagataa atatgaaaat gttaagtcac gtttaacact atattcatat atcgataagc
     1921 aagctgtacc aaataaaaaa gctgtaaatt taacattttc tactgcaggt agttctttat
     1981 cgaaagatgt aacagtagat tatcaagacc cgatggtaca aaataattcg aatattcaat
     2041 ctatctttac gaaattagac gaagacaaac atacaatcga gcaaacaatt tatgttaatc
     2101 cacaacagaa aaatgcaaaa gatactagag tggatattgc tgggaatgaa gttgatagca
     2161 atggtaattt caaatatggt aatggtggta ccataatcga ttcgaattct gaaataaaag
     2221 tttataaagt taagaagggt caacaaatac ctgatagcaa tagaatttat gattacagtc
     2281 agtatgaaga tgtaactagc caatttgatg acaaaaaaac tttcactaat aaccaagtaa
     2341 gattaaattt tggcgatatt gattctacgt atattgtaaa agtagtcagt aaatatacac
     2401 caacatcgga tggtgatttg aaaattattc aaggtacaag aatgattaca acgaatgatt
     2461 attataatac tgatgattat gcaggatatt caaacttcat cgtaagttct actgacactg
     2521 gcggtggtga cggtactgtt aaacctgaag aaaagttata caaaattggt gactatgtat
     2581 gggaagacgt tgataaagac ggtgttcaag gtacagattc aaaagaaaaa ccaatggcaa
     2641 acgttttagt tacattaact tacccggacg gtactacaaa atcagtaaga acagatgcta
     2701 atggtcatta cgaattcggt ggtttgaaag acggagaaac ttatacagtt aaattcgaaa
     2761 cgccaactgg atatctccca acaaaagtaa atggaacaac tgatggtgaa aaagactcaa
     2821 atggtagttc ggttactgtt aaaattaatg gtaaagatga tatgtcttta gatactggtt
     2881 tttacaaaga acctaaatac aacttaggtg actatgtatg ggaagatact aataaagatg
     2941 gtatccaaga tgcaaatgag ccaggaatca aagatgttaa ggttacatta aaagatagta
     3001 ctggaaaagt tattggtaca actactactg atgcctcggg taaatataaa tttacagatt
     3061 tagataatgg taactataca gtagaatttg aaacaccagc aggttacacg ccaacggtta
     3121 aaaatactac agctgatgat aaagattcta atggtttaac aacaacaggt gtcattaaag
     3181 atgcagataa tatgacatta gacagtggtt tctataaaac accaaaatac agtttaggtg
     3241 attatgtttg gtacgacagt aataaagacg gcaaacaaga ttcaactgaa aaaggtatca
     3301 aagatgtgac agttacattg caaaa
//
LOCUS       DAHHYJ010000189         3322 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000189 DAHHYJ010000000
VERSION     DAHHYJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.193"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            92..808
                     /locus_tag="NBK22_002477"
     CDS             92..808
                     /locus_tag="NBK22_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD2415632.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDEILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            808..1281
                     /locus_tag="NBK22_002478"
     CDS             808..1281
                     /locus_tag="NBK22_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCD2415633.1"
                     /translation="MKSAEQWIDELQLESHPEGGFYRETIREVLKDERRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYTTATLGTDIQNGDVLQYVVPK
                     GTIFASSIEISNTFSLVGCMCQPAFEFKQFELFKQSELITQYPHLKSVIEKYALK"
     gene            1373..1489
                     /locus_tag="NBK22_002479"
     CDS             1373..1489
                     /locus_tag="NBK22_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415634.1"
                     /translation="MLQLFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1634..2278
                     /locus_tag="NBK22_002480"
     CDS             1634..2278
                     /locus_tag="NBK22_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD2415635.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNIEKSKQIKPKQYNQTKNHQQQKKN"
     gene            2394..3260
                     /locus_tag="NBK22_002481"
     CDS             2394..3260
                     /locus_tag="NBK22_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2415636.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
BASE COUNT         1255 a          414 c          625 g         1028 t
ORIGIN      
        1 aataatgtta attgtgaaaa tgtatataaa cataaaaaaa tcatgtataa tatatgttgt
       61 taattaaaca gttcgaaagc gagatgacat tatgggacgt aaatggaata acattaaaga
      121 aaaaaaggcc caaaaagata aaaacacaag tagaatatat gcgaaatttg gtaaggagat
      181 ttatgttgca gcaaaatctg gtgaacccaa tccagaatct aaccaagctt taaggttggt
      241 gcttgaacgc gctaagacat attcagtgcc gaatcatatt attgaaaaag caatagataa
      301 agctaagggt gctggagacg aaaactttga tcacctaaga tatgaaggat ttggcccaag
      361 cggatcaatg ctaattgttg atgcgttaac aaataatgta aatcgtactg cctctgatgt
      421 gcgagctgct tttggtaaaa atggcggtaa tatgggtgta tctggatcag ttgcttatat
      481 gtttgatcat gtggcaacat ttggtattga aggaaagtct gttgacgaaa tacttgaaac
      541 attaatggaa caagatgtag atgtaaatga tgtgattgac gataatggat tgacaatagt
      601 ctatgctgaa ccagatcaat ttgcagtcgt tcaagatgcg cttcgtgcag caggtgttga
      661 agaatttaaa gttgctgaat ttgaaatgtt acctcaaaca gatattgaac tttctgaagc
      721 ggaccaagta acatttgaaa aattaatcga tgcattagaa gatttagaag atgtacaaaa
      781 cgtattccat aatgtggatt tgaaataatg aaatcagcag aacaatggat tgatgaattg
      841 caacttgaat cacatcctga aggtggtttc tatagagaga caattcgaga agtattgaaa
      901 gatgaacgca gagcgccgtt tagtagtatt tattttttac ttacagatga caatatttcg
      961 cattttcatc gaattgatgc tgatgaagta tggtactatc atgctggcga ttctctaaca
     1021 attcatatga taaatccgga tggggaatat acgactgcaa cattgggtac tgatatccaa
     1081 aatggagatg tattgcaata tgtagtgcct aaaggaacaa tttttgcttc ttcaatcgaa
     1141 atttcaaata cttttagttt agtaggttgt atgtgtcaac cggcatttga gtttaagcaa
     1201 tttgaattgt ttaagcaatc tgaattaatt acgcaatatc cgcatcttaa atcagtaatt
     1261 gaaaaatatg ctttaaaata aaaatgatca atgaagtggt ttgaaggttg ttaataaacc
     1321 tttgggtcac ttcattttta tatgtattct tgattgaatc agaatagatt tgatgcttca
     1381 gctgttttta atgaaaatag cattaaatga ttttgaaaac gataagagtg tgttatttat
     1441 atttttgaaa aatcactttt atgaagaaat gtgtgtacta tctaattaat attgtctatt
     1501 ttaagtaaat tgaagcgagt tgtagtaaaa taataataaa tattttcata tgattaacaa
     1561 aaactataaa actgtatcat gacatgacat catttatgta gttttatata ataatgacaa
     1621 ggagttggtg actgtgaaag taaagtatat agataaacgt cactggcgtc gcctaattga
     1681 tagggaatac acagaggtaa aagttaataa taataggttt aagggtatta taggcttagt
     1741 cacgatgaaa aaggttcgtg atcctttaga ggtgacggta gttggacaaa atatcattgt
     1801 cgcagatgac aattataaat ggttgcaaat actacctgaa aagaaacgtt atagtataac
     1861 tgtaatgttt gataataaag gcaatccatt agaatattat tttgatataa atatcaaaaa
     1921 tataacgcaa aaaggtaatg cgcgtacagt agatttatgt ttagatgttt tagcattacc
     1981 aagtggtgaa tatgagttgg tagatgaaga tgatttaatg tttgcattag aaagtgagca
     2041 aattacaaaa aagcaatttc atgaagcata tatgattgca catcaaatta tggcagagtt
     2101 agaaaatgat tttaaaggat tccaaaagaa aatcatgtac tgctttaata aaattaatgc
     2161 aaaggctcaa aaaaatcatc aaaagccaca aaataaaact aatattgaaa aaagcaaaca
     2221 aataaagcct aagcaatata atcaaactaa aaatcaccaa caacaaaaga aaaactaagt
     2281 aattcaagct gcagccatac caataaaatt ggtgaaatta ttcgttagta atgaactcat
     2341 gaatgcttgt agccatgaaa gttcaataat tgaataattt atggggggaa attatgaaga
     2401 ttgaagacta tcgtttacta ataacattag acgaaacgaa aacgttacgt aaagcggctg
     2461 aaattttata tatatctcaa cctgctgtta cacaaagact aaaagctatt gaaaatgctt
     2521 ttggagtaga tatttttatc agaacaaaaa aacaattgat tacaacaact gaaggaacaa
     2581 tgattattga gcatgcccgt gacatgttga aaagagagcg attatttttt gacaaaatgc
     2641 aggcacatat tggtgaagtg aatggaacaa tatcaatcgg gtgttcttct ttgattggac
     2701 aaaccttact tcctgaagtt ttgagcctat ataatgccca atttcctaat gttgaaatac
     2761 aagtgcaagt tggttcaact gaacaaatta aagcaaatca tagagattat catgttatga
     2821 taactcgtgg aaataaagta atgaatttag ctaacacaca tttatttaat gatgatcatt
     2881 attttatttt tccaaaaaat agaagagatg atgttacaaa gttaccattt atagagtttc
     2941 aagctgatcc gatttatata aatcaaataa aacaatggta taacgataat ttagaacaag
     3001 attaccatgc aactattaca gtggatcaag tagcaacttg caaagaaatg ttgattagtg
     3061 gtgtaggtgt tacaatcttg ccagaaatta tgatgaaaaa tatcagcaaa gaacaatttg
     3121 agtttgaaaa agtagaaatt gataatgaac cgctgattcg ttcgacattt atgagttatg
     3181 atccgagcat gttgcaattg ccacaagttg attcttttgt aaatctcatg gcgagctttg
     3241 ttgaacaacc aaaggcgtag ttttagacta atttaaggtt tgtatttaat tttaaactat
     3301 tcggttaaat tgaacgtagt tg
//
LOCUS       DAHHYJ010000190         3320 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000190 DAHHYJ010000000
VERSION     DAHHYJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.194"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(179..883)
                     /locus_tag="NBK22_002482"
     CDS             complement(179..883)
                     /locus_tag="NBK22_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCD2415637.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            1128..1322
                     /locus_tag="NBK22_002483"
     CDS             1128..1322
                     /locus_tag="NBK22_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCD2415638.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKAHH
                     FCINECSIGKEIKQLGNELQ"
     gene            1334..1585
                     /locus_tag="NBK22_002484"
     CDS             1334..1585
                     /locus_tag="NBK22_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCD2415639.1"
                     /translation="MKIISISETPNHNTMKITLSESRESMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            1623..2747
                     /locus_tag="NBK22_002485"
     CDS             1623..2747
                     /locus_tag="NBK22_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HCD2415640.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEETQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSDANKVLESVNEPQIDNHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTAHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHAINNYHFYRHVSLDEYHATDN
                     WKTRLRILNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDPQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            2763..3200
                     /locus_tag="NBK22_002486"
     CDS             2763..3200
                     /locus_tag="NBK22_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCD2415641.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVSEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQAP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
BASE COUNT         1222 a          510 c          591 g          997 t
ORIGIN      
        1 gatgacgaaa agtcagctta caataatgtg caagttgggg atgggcccca acaaagagaa
       61 attggattcc caatttctac agacaatgca agttggggtg ggacgacgaa ataaattttg
      121 cgaaaatatc atttctgtcc cactccctta aaacttattc ttttgtgtag taagtgcgtt
      181 aatagccttg atctaactta tcaatcttac ctttacgata aaatgattta gcaatatatc
      241 caaatggtac attgaaaact gttgaagcta attttaatac gtacgttgta ataaatattt
      301 caaatacaac tgtaccaggt aaacttccga taaatgcgat agctacaaat aaagctgtat
      361 caattattga gcttaaaaat gtactaccat atgcacggat gaaaaacgtt ttatcagaac
      421 taaatacttt tttaattagt gaaaagataa atacatcaat atgttgacca ataatatatg
      481 cgacgattga gcctaaagca atgcgtggca caacatcaaa gattgcgtgt aatgcttttt
      541 gtgccatatc ttctggtgca ggaataaaat gtaatgacaa ttgcataaca ataatcataa
      601 ttaatgttga tgaaaagcct aaccaaactg ctctttttgc aactctacgc ccataaatat
      661 cgtttaatat atctgttgct aaataaatag aagcaaacat gacattacct aaagttgctg
      721 aaataccaaa gatttctaca gttttaatca cttgtatgtt ggcaatgatt gtgccaattg
      781 caacccatgc aattaaacct tgtttaccaa aaaagcgata cataagtacc ataagcacga
      841 atgttgcaat aaacgtaact agtcctaaaa tttcattata catattaaaa tgtcctccta
      901 aattttgatc atgcgggtgt ttagaaaccg ctcaataaat aactaactca ttttactgaa
      961 aaatcgtttg cgatgcaata gcatttgaat ataaatacat tttacgtata gaattgtaaa
     1021 aggtttcatt caaatcttag ggtcaaaaat gttataatat ttttatgtca aatttaaaac
     1081 agtaacactt atttacaagg ttgcaatatt ttgaagtaat aaaggaagtg tcgcgtattt
     1141 taactttttc agagcaaaat gcactcgcga aaatagatga tttaatgaat acttattgca
     1201 atcaatgtcc aatcaaaact cgtctgcgta aattagaggg gaaaacgaag gcgcatcatt
     1261 tttgtatcaa tgagtgttca atagggaaag aaataaaaca attaggaaat gaacttcaat
     1321 aggaggaagt caaatgaaaa ttatatctat atcagaaaca ccgaaccaca acacaatgaa
     1381 gattacactt agtgaaagca gagaaagtat gacatcagat acgtatacta aagttgatga
     1441 ttcacagcca gcatttatta atgacatctt aaaggttgaa ggcgttaaat caattttcca
     1501 tgttatggac tttatttcag tagataaaga aaatgacgca aattgggaaa cagtattgcc
     1561 aaaagtagag gctgtattcg aataaatttt tcatcaacta gtattcgggg ggaataaagt
     1621 atatggaaat tttacgtata gagccaacac caagtccaaa tacaatgaaa gttgttttgt
     1681 catatacaag agaagacaag ttatctaata cttataaaaa agtagaagaa acacaaccaa
     1741 gatttataaa tcagttgtta tctatagatg gtatcacttc catttttcat gtcatgaact
     1801 tcttagctgt tgataaggca ccaaaagctg attgggaagt catattacct gatattaaag
     1861 ctgctttttc tgatgcgaat aaggttttag aatctgtaaa tgaacctcaa attgacaatc
     1921 attttggtga aattaaagct gaattattaa cttttaaggg tataccgtat caaattaagc
     1981 taacttctgc tgaccaagaa ttaagagaac aattaccaca aacatatgtt gatcatatga
     2041 ctcaagcgca aacagcacat gacaatattg tttttatgcg taaatggcta gatttaggaa
     2101 atcgctatgg aaatattgaa gaagtaatgg atggtgtcct agaagaagtg ctagctacct
     2161 atccagaatc acagttaccc gtattggtaa aacatgcttt agaagaaaat cacgcaatta
     2221 ataattatca tttctatcga catgtctctt tggatgaata tcatgcaact gataattgga
     2281 agactcgatt acgaatatta aaccattttc caaagccgac ttttgaagat ataccgctgc
     2341 ttgatttagc tttatctgat gaaaaagtac cggttagacg tcaagcgatt gtattattag
     2401 gtatgattga aagtaaagaa attttaccgt atttatataa ggggcttcgt gataaaagtc
     2461 ctgctgtaag aagaacagca ggggattgca taagcgattt agggtatcca gaggcactac
     2521 cagaaatggt gctactatta gatgatccac agaaaatcgt taggtggcgt gctgctatgt
     2581 ttatctttga tgaaggtaat gcagagcagc ttcccgcact aaaagcccat attaatgaca
     2641 atgcgtttga agttaaatta caaattgaaa tggccatatc tcgtattgaa aacggagatg
     2701 aagcacttgg ttcagtatgg aaacaaatgg caaacagaac aatttaattg gaggaattaa
     2761 atatgaatgc atatgatgct tatatgaaag aaattgcgca acaaatgcgt ggcgaattaa
     2821 ctcaaaatgg ttttacaagt ttagaaacga gcgaagcagt atcggagtat atgaaccaag
     2881 taaatgctga tgacactact tttgtagtta ttaactctac atgcggctgt gcagctggat
     2941 tagcaagacc agctgcagta gcagttgcaa cacaaaatga acatagacct acaaatacag
     3001 ttacagtttt tgctgggcaa gataaagaag caactgctac aatgcgagaa ttcattcagc
     3061 aagcaccatc tagtccttcg tatgctttat tcaaaggtca agatttagtt tattttatgc
     3121 ctagagaatt tatcgaaggt agagatatta atgacattgc aatggactta aaggatgcct
     3181 ttgacgaaaa ttgtaaatag tacacataaa taaatataaa ggttaacaca ttttataata
     3241 ttaaaaatgg tgtctgtcat tgaaaataga gaatatagtt gtattctatt tgttaaataa
     3301 agtccgtttt taccaactat
//
LOCUS       DAHHYJ010000191         3287 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000191 DAHHYJ010000000
VERSION     DAHHYJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.195"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..191)
                     /locus_tag="NBK22_002487"
     CDS             complement(<1..191)
                     /locus_tag="NBK22_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein"
                     /protein_id="HCD2415642.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNTQSVQAKTEVKQQSEADLKL
                     YYNGPSFEYKKVTGYGFIEG"
     gene            663..1508
                     /locus_tag="NBK22_002488"
     CDS             663..1508
                     /locus_tag="NBK22_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD2415643.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVRNVEKVPEDWRG
                     KVSVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQSGVAHII
                     FIGYYADQHNNPFHMSPYFGYASRLLATSGIDYTYVRMAMYMDPLKPYLPELMKMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKRYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAEMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            1527..1886
                     /locus_tag="NBK22_002489"
     CDS             1527..1886
                     /locus_tag="NBK22_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCD2415644.1"
                     /translation="MNIISTILIIFVALEFFYIMYLETIATTSKKTSETFNINVDKLK
                     DKNINLLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            2427..2579
                     /locus_tag="NBK22_002490"
     CDS             2427..2579
                     /locus_tag="NBK22_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415645.1"
                     /translation="MVPEEKGSITLSKEAAIIFAIAKFKPFKNRIKNNPQKTNPFLKL
                     HENKKS"
     gene            2840..3112
                     /locus_tag="NBK22_002491"
     CDS             2840..3112
                     /locus_tag="NBK22_002491"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2415646.1"
                     /translation="MTKERRTFSSEFKLQMVRLYKNGKLKNEIIRKYDLKPSIISNSI
                     KQHQNTGPFNHQDNLKSDEKELIKLRKEVQHLKMEHDVLKQILKLI"
BASE COUNT         1201 a          503 c          497 g         1086 t
ORIGIN      
        1 ccttcaataa atccatatcc agttactttt ttatattcaa aacttggtcc attataataa
       61 agttttaaat cagcctcact ttgttgttta acttctgttt tcgcttgtac tgattgtgta
      121 ttcgacgtaa ttacacctgt tgctaacatt cccaatgcca aacttgcttt tgctatcgct
      181 ttaaatttca taatttttag ccccaatcta tttaaatttg atttttcaat tacgtaatat
      241 gaacaatcta cctacattat attgaggcgc tatccccttt caaattaaac ctcatttaac
      301 taaacttgaa acattgttaa ggtaattaat aaaatgctaa ctgttattaa tcaattctta
      361 tccatcgaga ctcattttta taccattcac actttattcc aatttaattt taaaaactgt
      421 aaaactttca gacacaaaac agacatcata ttgtcgtagt acctgattaa aattcccaaa
      481 gatattagtg atacacatca actaaaacgc gtcattatgg tattttcaac attaataaca
      541 tatacgtatt tactatacac gacgacaata aatacagatt acaatttttt ataaaatcaa
      601 atgttttgaa aaccatcgaa taagtattat gttatgtata taaagattag gagatgaagt
      661 gaatgaatat tatgttaaca ggtgctacag gtcatttagg cacacatatt acaaatcaag
      721 ccattgcaaa tcacatagat cattttcaca ttggtgttag aaatgttgag aaagttccag
      781 aagattggcg tggaaaagtt tctgttcgac aattagatta ttttaatcaa gaaagcatgg
      841 tagaagcatt taaaggaatg gataccgttg tgtttattcc aagtattatc catccatcat
      901 tcaaacgtat tcctgaagtg gaaaatttag tatatgcggc aaagcaaagt ggcgttgctc
      961 atatcatttt cataggttat tacgcagatc agcataataa tccattccat atgagccctt
     1021 atttcggata tgcatcacgt ttattggcaa caagtggcat tgattatacg tatgtaagaa
     1081 tggcgatgta catggatcca cttaaaccat atttaccaga attgatgaag atgcataaac
     1141 tgatttatcc agctggtgat ggtcgtatta attatattac tagaaatgat attgctagag
     1201 gtgtcattgc tattattaaa aatccagata cttggggcaa acgctattta ttatcaggct
     1261 acagttatga tatgaaagaa cttgctgcaa ttttatctga ggcatcaggc acagaaatta
     1321 aatatgaacc cgtttcatta gagacatttg cagaaatgta cgatgaacct aaaggctttg
     1381 gtgcattatt ggcatcaatg tacgacgcag gagcaagagg actattagac caagaatcca
     1441 atgatttcca acaattagtc aatgatcaac cacagacact gcaatcattt ttacaagaaa
     1501 atatttaata ataaaggagc gttacagtga atatcatctc aacaatttta atcatatttg
     1561 tggcattaga gtttttctat attatgtacc ttgaaacgat tgctacaact tccaaaaaga
     1621 ctagcgagac atttaatata aacgtcgata aattgaaaga caaaaatata aacctacttt
     1681 tgaagaacca aggcgtatat aacggtttaa tcggagtttt gctaatatac ggtttgttta
     1741 tcagcagtaa tccaaaagaa atatgtgcag ctattttagt gtatatcatt ggcgttgcta
     1801 tttatggtgg cctttcaagc aatattagta tctttttcaa acaaggcaca ttgccagtat
     1861 tggcactcat atcaatgctt tggtaagtat tggtgtttgg gggttgggag atgtagtctg
     1921 ggtttggagg gtttggaggg tttggagggt ttgagcgatt tgggtatgga ctttagactc
     1981 agagtatttc atcctaatta tttcaagcag aggtgacagt agcgttgcct ctgtttcctt
     2041 atagaaaaat gatttaatga agaaaattaa tatctgtttt aatttttagc tgataaattc
     2101 tccatatatt agaatggcta ctttatctat tgcatcaatc ctttaaaaca aaaaacccat
     2161 gatttcaaaa ttcccgtatg atggagttcc tactctcatg gatcagttaa ataaatatta
     2221 tcactatcag tttattattt caatattatt aacaatatat gtagtcgtaa aaggaaagag
     2281 gacatgagaa cttcggtgtt gattggcatt acataacgct tccaaacata ttatttggta
     2341 acaataagaa actatttaca caatatattt tgtatagtaa aattatttta taatatttaa
     2401 atccaattgc acaaggagtg attatcatgg taccagaaga aaaaggttct attactttgt
     2461 caaaagaagc agctatcata tttgcaatcg caaaattcaa accatttaag aacagaatta
     2521 aaaataaccc acaaaaaaca aatccatttc ttaaattaca tgaaaacaaa aaatcttaat
     2581 cactttattt atagcatttc taatctcaga aatgctataa aggacaacgc cctctttcat
     2641 aactatataa gtcaagaaac aaagacattt caaacatctt taaacccttc aatagcacat
     2701 tccctaaaac ataaagcaat aacaatgaac gatcaaaatt ccttacgtac ttatgatgtg
     2761 tcagtgaatc gtctacactt aattgaacaa attctatgag aatagatatt gttaaattaa
     2821 gaaagtagtc aattttatta tgacaaaaga aagaagaaca tttagttcag agtttaagtt
     2881 acaaatggtt agattatata aaaatggtaa gcttaagaat gaaattatac gaaagtatga
     2941 tttaaaaccc tcaattatct caaattcgat aaaacaacac caaaatactg gacccttcaa
     3001 tcatcaagat aatttaaaaa gtgatgaaaa agagttaata aaattacgca aagaagttca
     3061 acatttaaaa atggaacatg atgttttaaa gcaaatttta aagttgattt agaaactgaa
     3121 ctttttaaag atacattact tgaagattct aatttatctg attatataaa gaaccaactt
     3181 ccaacgatat ggagagacta agaaatagtt taaatttatt taatttcaat aaaaactcat
     3241 aataatattt aagtaagtta gattgctttt agcattagga attcgct
//
LOCUS       DAHHYJ010000192         3280 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000192 DAHHYJ010000000
VERSION     DAHHYJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.196"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..127)
                     /locus_tag="NBK22_002492"
     CDS             complement(<1..127)
                     /locus_tag="NBK22_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002492847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD2415647.1"
                     /translation="METVYSIHKNPRYKVIEIDNRYLMVDLTQNWYSYICPMLNWF"
     gene            complement(111..473)
                     /locus_tag="NBK22_002493"
     CDS             complement(111..473)
                     /locus_tag="NBK22_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415648.1"
                     /translation="MSNKLDEINKIITAKHKQMDDLYDEKRVVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTEALYEQQQQIQHGIRKAEEEMIDL
                     EMRRNVEIETVTCNGNRI"
     gene            complement(488..766)
                     /locus_tag="NBK22_002494"
     CDS             complement(488..766)
                     /locus_tag="NBK22_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCD2415649.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            complement(1103..1969)
                     /locus_tag="NBK22_002495"
     CDS             complement(1103..1969)
                     /locus_tag="NBK22_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCD2415650.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRMDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTS"
     gene            2187..3251
                     /locus_tag="NBK22_002496"
     CDS             2187..3251
                     /locus_tag="NBK22_002496"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCD2415651.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTTFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRPLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
BASE COUNT         1079 a          644 c          464 g         1093 t
ORIGIN      
        1 taaaccaatt aagcattgga catatatatg aataccaatt ttgtgtcaaa tcaaccataa
       61 ggtatctatt atcaatttct ataactttgt atcttggatt tttatggata ctatatacgg
      121 tttccattgc aagtcaccgt ctcaatttca acattccttc gcatttccaa gtcaatcatc
      181 tcttcttccg ctttacgaat gccatgttga atttgctgtt gctgctcgta taacgcttcc
      241 gttgaacgtc gtttcgcaaa atgaaattca tctcggaact gttccatacg actagccata
      301 ttgctactat aataacgctc acctaaatgt tgatataatt gatctatcga atgattaagc
      361 gcatcacttt catctatcaa tgctttaacc actcgctttt catcatataa gtcatccatt
      421 tgcttatgtt tcgctgtgat tattttattg atttcatcca gtttattcga catcatttca
      481 ccacgtttta atttttacca atttcattac caatcattac gtcagtttgg tcaaaagcct
      541 caccaacttt ttgtatatta tcgctcgcag ttcgtattgc ttcagcaaca gattgattct
      601 tactcatcaa atcatctatc acactatgag cattattatt tccacttact gttgtacttt
      661 catctttatc cggtttcgac acattattac atgtttcaac agcgctttta atagatgatg
      721 atgcactatt agtcttgaaa atatcacttt gaactgtact catcatttac acatctctcc
      781 atttaaatta ttttaaaatt attagaaact gtatcaatat atgatattca aacattcaat
      841 tatacttaaa tattgtttta tgattatatt caaaattatt gtatcaataa atatgtatca
      901 ttcctagaat tttattgaac aaaaagttat aaaatcactt ttattgtaac tcaaccttgt
      961 tttacattac agaaattaac aatagcgatt aaaattttat tttccaacaa taaaaaagca
     1021 caaatatttt tattgaggca gctgaaggcc ttctcatagc aaaaatatct gtgcatatta
     1081 ttatctcaat ctaacgtaat aactaacttg ttgtatcttg taaaatctta tccatagaat
     1141 gcgcaacact atcacggtac atctcaccaa atttcaataa atggaccatc aataaatata
     1201 aacgataaaa ttcaagtcta taggatgcac cttttgcgag tggataatgt ttattatacg
     1261 catcataaaa ttcactcgta aaaccaccaa ataccgttgt aataccgata tcgaattctc
     1321 tgtcaccata taatggcgct ggatcaaata acgccggacg accatcttgt aagaacatat
     1381 aattaccacc ccataggtca ccatgtaata aagacggttt actttgatgc ttttctaatt
     1441 ccgccacaat ttgacgtcgc actttgtcat atactttgat atcgttggca tcccatagcc
     1501 ctctatttag caactcatct ttcaaatggt ccatgcgctt gtcgacaaaa atggtacacc
     1561 aatcgtcttg ccaatgatta tcaaaagaaa tatcgccacc ttcataaggt aatgagaagc
     1621 caaatttgcc ttcttcttgt tgctgactgt gtaattgagc tacgagttgc cctaattggc
     1681 gttgactccc tgaagcccct tcttctaaat acgtcatcac taaatacgca tcaccgttaa
     1741 cctcgccact tgcaattact ctaggtgccg tgatacctgc acgttcaaat tcatttaaac
     1801 ctgcaatttc tgcagcataa aatgattctt tacgtccacg ttggacaagt aagaaaaatg
     1861 tatccgtatc tgtttcgact cgatatgctt cgtttacatc accaccactc actggtgaaa
     1921 tctctttaat atcttttaaa ggtaaatgct ctaaccattg ttcattcata caaacacctt
     1981 ccaatctact tactttatat tataatattg cctaattagg atgatttaat cttaaaaacg
     2041 ttattcaaac ttgcgtcaat tttcaaaact ttttagtttt aactaggaaa ttatgtaggg
     2101 aaatgctagt ctattaaaag aatatatttc acatatatta agttgaggat agtaagcctc
     2161 aaatataatg aaataggagc atactcatgt acaaattaat taaacctttc ttattcaaaa
     2221 tcgaacccga aaaagcacac ggactaacta tcgatgcatt aaaaacgtta caaaagtttc
     2281 cggttttatt cccagtcgtc gataaactat ttacttataa gaatccaacg ttatcacaaa
     2341 cgatacaagg taatacgtac gacaatccaa ttggcttagc agctggtttc gacaaatctt
     2401 gcgaagtacc aaaagcattg gaacaccttg gattcggtgc tttagaatta ggtggtatca
     2461 cacctaaacc tcaaccgggt aaccctcaac cacgcatgtt tagattatta gaagatgacg
     2521 ccttgataaa tcgaatgggc ttcaataata ttggtatgaa caaagcacta agtcatttgc
     2581 gtaaaaatgc ttatcaagta cctgttggta tcaatgttgg tgtgaataaa atgacacctt
     2641 atgaagcgcg ttatcaagat tatataaaag tcattgatac gtttaaacac gacgtttcat
     2701 ttttcacagt caatatcagt tctccaaata ctgaaaacct tcaaaatttc catgataaag
     2761 atgaattttc aatgttatgt caagctttga caacatttaa aaaacaacat gatgtaacag
     2821 tgccaattta tttaaaacta acgtctgata tggatttcga tggcttaaaa gcactattac
     2881 cagcgattac tgagacattt gacggtatca tcttagcaaa cacaacgcga caacgagatg
     2941 gtttaacttc tgctaataaa gtcgaagaag gcggtttgag tggtcgtcca ttatttgaac
     3001 gtaatttaaa attgattaag tatgcttatc agcaaacaaa tggtgaattt ttaattatag
     3061 gtacaggcgg cgtattcagt actgaagatg caatcaaaat gatgcgtcac ggtgcgtcac
     3121 ttattcaaat ttattcatca cttgttattg aaggcccagg tttaactaag aaaatgaaca
     3181 aaggcatcgc acgttactta aaagatcatc attttgacaa tgtcagtgat attataggac
     3241 tagatgccta atccaacata gtcaaataat actttaaaac
//
LOCUS       DAHHYJ010000193         3236 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000193 DAHHYJ010000000
VERSION     DAHHYJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.197"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            190..759
                     /locus_tag="NBK22_002497"
     CDS             190..759
                     /locus_tag="NBK22_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415652.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLQPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            847..1131
                     /locus_tag="NBK22_002498"
     CDS             847..1131
                     /locus_tag="NBK22_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415653.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNNGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1193..1411
                     /locus_tag="NBK22_002499"
     CDS             1193..1411
                     /locus_tag="NBK22_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415654.1"
                     /translation="MSEWHINNQSTKPFLIQQAEKQLSIVKPLPNGSFQILTEINLEN
                     GNISNIDHSLLVSVDPKALEISIFDAVN"
     gene            complement(1907..2107)
                     /locus_tag="NBK22_002500"
     CDS             complement(1907..2107)
                     /locus_tag="NBK22_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD2415655.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
     gene            complement(2464..3150)
                     /locus_tag="NBK22_002501"
     CDS             complement(2464..3150)
                     /locus_tag="NBK22_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCD2415656.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
BASE COUNT         1120 a          523 c          424 g         1169 t
ORIGIN      
        1 aaatgaaatt cattttatat ccagtctgaa acgtctcaat gatgtttcag actgtttttt
       61 attcttaata gacatttcac actctcatac tttaatattt aagatatgtt ttagtcttac
      121 aagcttatta aagattacaa ttctaattta agactattta catatatttc ttcaacaagg
      181 agcttttaag tgaactatat taaacgaacg ataattttac ttatattatt cgttgtcgta
      241 tccccaataa actcaccaaa aacaattgca gataataagt attcagaaat tcaagatgac
      301 aaattccaat tacaacctgg tgatattatt gttacaaaag gccctgtcat gtggggattt
      361 tttggtcatt gtagtatcgc gattgatgat aaaacgattt tacaaattga ggggcctggc
      421 gacaaaccaa ctacacaatc cttcgaatct tttaaatata attatgcaag tggcaaaaat
      481 gattggatga aagtttatcg ttgcagctat cctggtgcag gtaaaaaagc ggctgaatgg
      541 gttaagaaaa actatgagaa tacaaatcat cgttatctag ttacattaaa tttgaacagc
      601 aaaaaattta cgtattgtac taaaattatc taccaagctt ataaatttgg tgtaagtgaa
      661 aaatcagtta aaagttatgg actacatatt atttcaccct atgcaattaa agataatttt
      721 atagatccat acaaactaag gcttgttaaa gcttattaac acagtttcat caaatagtta
      781 aattagtttg tgttttgcaa taaattgggt atagattaca aatgatattt caggaggccc
      841 aatattttga atgaactaac taaagaacaa aagtatacaa tagctaaatt ttacaaatta
      901 tatattgaac gttcgaataa tggagaaaca gaaacagtcg ctaacttttt tggcgatgct
      961 aaagatgcac gtgaaaacta tttttgtgat cgtgattatc aagacttctt aactaactgc
     1021 caaattttaa ttcaaaacaa atacttaact ggtgaagttt tggacgataa catttacaat
     1081 atttctattt taaacaagac atttattgaa ttcgaacaaa attttggttg aacatgaatg
     1141 tctcaactat aaactttcta accctatttt aaagcaggag tgaaaaacat acatgagtga
     1201 atggcatatt aataatcaat ctacaaaacc ttttttaata caacaagcgg aaaagcaatt
     1261 gtctattgtt aaaccgttgc ctaatggtag cttccaaata ctcacagaaa taaatttgga
     1321 gaatggcaat attagtaaca tcgatcattc tttacttgtt tcagtagacc ccaaagcatt
     1381 agaaataagt atatttgacg ctgtaaatta aacgcaatat ttttatttaa ttattttaat
     1441 gtttcgcctt ttaaatgaaa acaagattac tgaattatta tgagatttta aaaacaataa
     1501 atattctcaa taattaatca gtcttaataa aaaattaaca aaaagttgat tagttaaatt
     1561 attaattaag tagtaagata ttatgcgatg tggtatccta ttattgttaa acattttgag
     1621 tgtttaacat tacttcttgt tatcgccata tacagacttt aattctcgtc attattaaag
     1681 ttctgtattg gcgctttttt atattcaaaa tcaaaagtcg gacagatgaa aagtaaaaaa
     1741 cttttccatc agttcgattt attttagaac cattatatat tgttagatct ccacctaaat
     1801 ttattggaca aaaaaataac agctagtgct tttaccacac caactgttat cgatttaaag
     1861 cataaaaaaa cagccctata ataaggactg tttgaataag ttaaaattac attttaacta
     1921 cgtttgcagc ttgctcgcca cggtcgcctt caacgatgtc gaattcaact ttttggcctt
     1981 cttctaatga tttgtatcca tcttcagcga ttgctgagaa gtgtacgaat acgtcgctac
     2041 catcttctct ttcgatgaaa ccaaaacctt tttctgcatt aaaccattta actgtaccgt
     2101 tattcatatt gaatacctcc gtcgtgcttt tgcactaaat attttgtaac aaattcataa
     2161 atgaaaagga gaatattcta tataaataac tcacattttt cttcacgctc tttattactt
     2221 aactttcatt atacactttt aaatatagaa gtaaagcatt attttatttt ctgtgataat
     2281 acacattgaa atatatagta atattccatt ttaatactaa atttaaaatt tttattttct
     2341 aatattaaat acacttgctt ttgatactat ttactttttt aattctgaat tgataataaa
     2401 tcatatcgtg atccaaaaat tataaaagtg ccactagcag cagtgacact tttacaatga
     2461 gcattattga cctgaatcag cgttgtcttc gctccaaata tttaatttct cttttttcgc
     2521 ttgtgcttca ctttttctta aaagttgttc atgtgtattg ttaggtttat aaacataagc
     2581 aactttagcc aagccttgac gaactaaagc ttcgtttacc atttttccat cagcataaat
     2641 atacgctaag ccacgtccat atttatcagt tctttgacct ttgtcaaact cgacttcaat
     2701 tttctttgca ttttctacca tttttttcgt aaatgcactt gcttcaggac catatttctc
     2761 tacacctttt ttaggatgct ttgtttcagg tgtatcaacc aataatagtc taaatgtcat
     2821 tggttgacct ttgtacatta atttaaccgt atcaccatca atcgctttaa ttaaagtcgc
     2881 aggttcttta tgtaattttt tagttgaagt tgcactatat actgttggat cttcagaacc
     2941 acttctattt acgccgttat ctgtttgtga tgcatttgct gagctactta gacttgaaac
     3001 tacaactaaa gttaacacta agcaactagt agcgaaaaag aaaaacctct ttgcgtattg
     3061 ccctttcgaa acattactga tagccatccc tataagtaat attgaaacaa ttgccataca
     3121 tatgccagca cttaataagt attctgtcat aactaacacc tctttctttt tagttaattt
     3181 taatattaaa cgttaattaa ttataattct acatttttac aattaattaa taaaaa
//
LOCUS       DAHHYJ010000194         2958 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000194 DAHHYJ010000000
VERSION     DAHHYJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.198"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(192..1127)
                     /gene="trxB"
                     /locus_tag="NBK22_002502"
     CDS             complement(192..1127)
                     /gene="trxB"
                     /locus_tag="NBK22_002502"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCD2415657.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
     gene            complement(1194..2633)
                     /locus_tag="NBK22_002503"
     CDS             complement(1194..2633)
                     /locus_tag="NBK22_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD2415658.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMAKQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNIKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYQNELFVSENDLMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKKQITTWLGITQYKLNKMIEFLLSI"
     gene            complement(2641..>2958)
                     /locus_tag="NBK22_002504"
     CDS             complement(2641..>2958)
                     /locus_tag="NBK22_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD2415659.1"
                     /translation="NIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVD
                     EFRYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKM
                     IRR"
BASE COUNT          955 a          568 c          380 g         1055 t
ORIGIN      
        1 aagttataag tttaatataa catataaaaa aactagaact cattgaaacg cacaaatgat
       61 acgtactaag ttctagtttt ctatataatt tgctttaatt taaaagtaag acattatttt
      121 aacaatgtga tgacactatt aaaataaata tccaaataac ttacaactca acatcttaat
      181 tcgacctcga attaagcttg atcgtttaaa tgttcaatat attccgctgc actttgcgct
      241 gcaatactgc catcaccagt agcagtgaca atttggcgta aacctttgtc gcgaacatct
      301 cctgctgcaa aaatacctgg tactgatgtt gtcatatcat cttttgttac aatataacca
      361 acatcatttg taatacctaa gtctttaaat ggcgctgtta atggtttcat accaatatag
      421 atgaatacac catcagcctc gtgtgtttct tctgaaccat cttttgtaga cgttaatgtc
      481 acagaaccca ctttgccgtc tttttcatta attgatttca aagtatgact ccaaataaag
      541 tcgattttat catttttgaa tgctctatct tgtaaaatac gttgtgcacg taactcatca
      601 cgacggtgaa cgattgttac tttgtcagca aatttagtta agaatgttcc ctcttctact
      661 gctgagtcac caccaccgat aacgaatagg cgtttatttt taaagaatgc accatcacat
      721 actgcacaat aacttacacc gcgtccacca agttcttgtt cacccggaac accaattttc
      781 ttgtattctg cacctgtagc aataatgacc gctttcgctg ttaattcttt attaccaaag
      841 ttaatcactt tatattcgcc tttatcttct acagatttaa tatctccata ttgataaact
      901 gcaccaaact ttttagcgtg ttcaaacatt tttgtagata aatctggacc tgtaatcatt
      961 tcgaaaccag ggaagttctc tacttcttct gtattagcca tttgaccgcc tggaatacct
     1021 ctttcaatca taactgtttt taaattagca cgtgatgcgt atactgcagc agtcatacca
     1081 gctggacctg caccgataat tgctatatca aaatctattt cagtcatttt attaacgcct
     1141 cctcattatt aatcattatg cgcattatat aataaatcta acttttcata aatctatatg
     1201 ctcaagagaa attcaatcat tttgttcagt ttatattgtg ttatgcctaa ccatgttgta
     1261 atttgcttct ttgtaacgtt tcgaggttga tttttaaaat acaaataaat aaacgcaccg
     1321 atatatggct caacatcagt taaatctact ttttcagcaa ttatgagttc accttgatta
     1381 atccatgcaa ccattaaatc attttcactt acaaataatt cattttggta aagcgttaac
     1441 aagccgcgat gaatgaagtc taatttattg agcgttaaac cttgaactaa atacgttaaa
     1501 tacaatttct cataattatt tagattttcc agaacctgcc aaatactttc cgtcatcaca
     1561 atttctttac catttaattg atccaataaa aaaataccat ataaacgata atgactgtca
     1621 tcactttgaa gtaatggcaa aatatgttgg tcaaaaactt ctttgctatt ttcaattacc
     1681 caaggcgcat gtccaatttc cactttagaa atttgcttca atttatccca gtagtaatga
     1741 ctttcgtctt cttcacctaa ataataataa ttatatgcta aagcattgta catttgaatt
     1801 gataaaaatt tccctttttt atataaaggg tacaacaatt gttgtgatgc acgatactgc
     1861 tttaaataac ttaatacaat acctaattta aaactttcat cgtcattcat aggtacaact
     1921 ttgtttaata tttttaaata tttttgatat tgttcatttt ccttaatgtt ataaagtagc
     1981 aaagtataat ggcataatgc atatatatct gtactatcct cttgtaatag tgcttcgtac
     2041 aatgctttag ccgtatcata ttcaccgaga tataaataac acattgccaa taaattacgt
     2101 acgacacgat gatcttgaac ttcttgtggt tgatgtaaga cataattctt tgcatctttt
     2161 aatcgacctt gagaaaaaag atattggaat agtatttgaa ctacaaacaa ttgagcttca
     2221 agttcaattt gttcttcgct ttgatatttc acatcaaaca tttgatctaa ttcatctcta
     2281 taatcttggt cgtcgctaac aataacataa ttaataccaa acaagaatgc cttgtttggt
     2341 tcgttaactt caatgttgag ctgactcaat tcataaaagc tatcttctag atgcctatta
     2401 taaataatat tgtcataaaa taaatgttct gcttttttag caataccaag ttgcactaga
     2461 cattgtgcat aatcaatttg aatttccaga tcatcaggtg acaattctaa cactttttca
     2521 aagtattcag cagctcgttt atattctctt tgtttaaact tctgtttagc cattttacga
     2581 taaaatgcat catcaaaagt cattggaatt acattattat tcttttgcgc cataaaattg
     2641 tcacctccta atcattttta tatacatagg gttgccatat gcaaatccat tatccggtat
     2701 atcttttgaa acaaccgtac cagctgcaac ttttacattg tcaccaatcg ttataccggg
     2761 taaaatggta gcatttgcac caatcaaagt gttagatcct atcgtcactg gtccataacg
     2821 aaattcatca actaatgctt catgcgtcaa aattgttaca ttgtaaccaa taacactgtt
     2881 actaccaatc gtaatcagtt ctgggtaaaa aatatctaac attactttat aagctatcga
     2941 cgattgatta ccgatatt
//
LOCUS       DAHHYJ010000195         2877 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000195 DAHHYJ010000000
VERSION     DAHHYJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.199"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            113..484
                     /locus_tag="NBK22_002505"
     CDS             113..484
                     /locus_tag="NBK22_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCD2415660.1"
                     /translation="MLKKWLGMALITPILTFIIWLFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            complement(539..1783)
                     /gene="fabF"
                     /locus_tag="NBK22_002506"
     CDS             complement(539..1783)
                     /gene="fabF"
                     /locus_tag="NBK22_002506"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCD2415661.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINDNTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLGAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1795..2736)
                     /locus_tag="NBK22_002507"
     CDS             complement(1795..2736)
                     /locus_tag="NBK22_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020369794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCD2415662.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASIKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
BASE COUNT          876 a          573 c          427 g         1001 t
ORIGIN      
        1 aatgattgat tttagaattt caataaagct gaagaaatta aaaaatcatt cctcaatgtc
       61 tgattttaat gtaaactatg cgtaagagtt ttctataagt gggagtgatc aaatgttgaa
      121 aaaatggctt ggaatggcac taataacgcc aatattaacg tttattatat ggctttttaa
      181 tagccatact atcattacat atttaaatat attgttttat gtttcattaa ttatttttat
      241 tagcatattt ttgattttac ttgtacaaga aggcattttt gatgcgacaa gttatggttt
      301 tagaagatta aagtatcaaa tgtcatcttc gaaaaagaag aagtctatct ctgatgatcc
      361 atttttcaac ccacaagagg taaaaaaaga gcattacttt gtttctacat ggataatccc
      421 cttattatta ataaatattt tatattttat tatgacgatt gtgctgtcgc tcatcttagt
      481 ataaagaaac tgaaaaaatc ttcagtaact aaaacaacgt tactgaagat ttttatagtt
      541 atgcttcaaa tttcttgaat actaatactg cgttatgtcc accgaatcct aagctattac
      601 tcattgcata agtaatatca aggtcttgcg cttcatttgg aacaatatcc aaatcacatt
      661 ctggatctgg tgttaccgca tgaattgtcg gggcgacttt agagtcttta attgaaagcg
      721 ctgagaagat tgcttcaatt ccacctgttg caccaagtaa gtgaccagtc attgattttg
      781 ttgagctaac ttttaagtgt ttagctgctt caccaaatgt atttttaata gctttaactt
      841 catttaagtc accaacagga gtacttgtac catgggcatt taagtattgt acatctttag
      901 gttcaatacc agcatcatcc atagctgctt gcattgctct agaaccacct tcaccttctg
      961 gagctggcgc tgtaatatga taagcatcac ctgtagtacc atagccaact atctcagcat
     1021 aaatattggc acctcgagct tgtgctgatt ctaaagattc aattactaaa ataccagcac
     1081 cttcacccat aacaaaaccg tctctacctt cttggaatgg acgacatgct gtttcaatgt
     1141 catcatttgt agaaagcgct cgacttgcac tgaaaccagc aattgccata tgagtaattg
     1201 gtgcttctgt accaccagta atcattgcat ctgcatcacc gcgttgcaca attttaaatg
     1261 cttctccgat tgaatttgta cctgttgcac atgctgtaac tgttgcacca tttggtcctt
     1321 ttgcacctaa gtcaattgat acttgcccag ttgccatatc aggaattaac attggtacga
     1381 aaaatggact cacacgtctt gggcctttat ccattaattg tttatgtgca atttcaaatg
     1441 tttccatacc accgatacca gaaccaatcc atacaccgat tcgatctgca gtattatcat
     1501 tgatatctaa ttgcgcatct ttaacagcct ctctagctgc tacaattgca tattgagtaa
     1561 atctatccat acgacgcgct tcttttttgt cgatatgatc ttcaatatta aagtttttaa
     1621 gttctcctgc taagtgaacg ctataaggtt cagtatcgat acgtgtaatt ttatcgatac
     1681 catttacgcc ttttagagca ttctcccatg ttgttttgac atcattaccg attggagaaa
     1741 gggctcccat acctgtaata actactcttt tattttgact cattcgttat cctcctattt
     1801 tccccatttt attgtcattg cgccccaagt taggccgcca ccgaatccga caagaacaat
     1861 tgtatcatca tctttgagtt taccattttt taattcttga tcgatactta aaggtattga
     1921 cgcagctgaa gtatttccat atttatttac agaaacactc attttgtctt ttgaaatacc
     1981 taagcgttct ctagctgatt ccataattct aatattagct tgatgaggaa taaataaatc
     2041 tatatcatct gatgttaaat tcgctttttc aactacacgt gttgatgcat cacccataat
     2101 tctaacagca aatttaaata cttctcgacc attcattttc agtttaccag tatctttatc
     2161 taaatataaa tgtttaccac cagtgccatc agaacccatt tcataactta taatacctct
     2221 gccttctgaa acttcaccga tgataaccgc acctgcacca tctccaaata gaactgcagt
     2281 agaacggtca gttaaatctg ttattttaga taatttatct gcaccgacaa ctaaaatatt
     2341 atgataatct ccagattgaa catattgttt agctgtaatc attgaataca taaatccaga
     2401 acatgctgca agttgatcca tagaggcaac tttgcccgtc cctaaacgtt cttgcaacat
     2461 atttgcgaca gttggaaatg gcatatctcc agttgctgtg gcaacaatta tcatatctat
     2521 atcttcgggc tgaataccag cgtcagcgat tgcttttata cttgcttcat atgctaaatc
     2581 tgaagtatct tgatcgtcat ctgcccaatg tctttcttta attccagtca tcttagaaat
     2641 ccattcatca gatgtatcta aaaattgctc aaaataggca ttgtcaataa tcttttctgg
     2701 tgcatatgca ccaaaacctt taatacccac gttcatagtt gaacaccttc ttaataaaat
     2761 ttttaatacc aggtattaat ttaacataga ttacttttta aactcaagtc agaggtagga
     2821 atattgacta gttaataaaa ttttaatgct ttattatcgt caaatttcaa tatgaaa
//
LOCUS       DAHHYJ010000196         2849 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000196 DAHHYJ010000000
VERSION     DAHHYJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.200"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..966
                     /locus_tag="NBK22_002508"
     CDS             <1..966
                     /locus_tag="NBK22_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCD2415663.1"
                     /translation="GISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQK
                     STIRLYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEV
                     NTSLQSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMM
                     HMVQDMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGI
                     KCVMIAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAITRFRQGLGRLIRSENDRGII
                     VSFDDRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            1288..2580
                     /gene="asnS"
                     /locus_tag="NBK22_002509"
     CDS             1288..2580
                     /gene="asnS"
                     /locus_tag="NBK22_002509"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCD2415664.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKILERDTSKLEKVATPFPR
                     ISYDDAIEFLKSEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
BASE COUNT         1060 a          381 c          493 g          915 t
ORIGIN      
        1 ggcattagtc ataaaacagt taaatcatta cggaaacagt tgctgtattt aaaagataaa
       61 tttaaaaata tcgaacaaag tttaaaagct ggacatacta gtttcatttc gattaaaaat
      121 ttatctcaaa aatcaacaat acgtttatat gtgaaagatt acgctgtaaa agatgtatta
      181 acgaaacaag tattggaaaa atttaaatca ctcattttta tatctggtac tttaaaattt
      241 aatcattcgt ttgaagcttt taaacagtta ttcaacaaag atgttcactt taatacattt
      301 gaagttaaca cgtcgttaca aagtgcaaaa aatacaagtg tatttatacc gagtgatgta
      361 gcatcttatc aatataaaaa tatagatgag tatgtagcat cgattgtaag ctatattatt
      421 gaatatacga ctataacgtc atcaaaatgt ttagtcttat ttacgagtta taaaatgatg
      481 catatggtac aagatatgct aaatgaattg ccagaatttg aagattatgt tgtattaaca
      541 caacagcaga accaaaatta taaaatagta caacagttta ataattttga taaggccata
      601 ctgcttggta cgtcaacatt ttttgaaggt tttgattttc aagcaaatgg tattaaatgt
      661 gtgatgatag cgaagttacc gtttatgaac aagcataatg caaaatattg gcttatggat
      721 tctgaattca cttcaacttt caaggaatat gtattacctg atgcaattac acgttttaga
      781 caaggacttg gaagattaat tagaagtgaa aatgatcgcg gcataatagt ttcatttgat
      841 gatcgactta taaacagtaa ttataaaaac ttttttgaac aaacacttga aaattatcgt
      901 cagaaaaaag gcgacattca acagtttgga aaattattaa gacaaattca aaaaaagaaa
      961 aagtgaaata caaagtaaaa ttcttgtgac atgacatgtg ctatgttctg ttacaagaat
     1021 ttttgtttgg tcgtcaaaat ttgagtttta taattaaact tgaatgttag cacaaaaaac
     1081 aaccttcata ctaataatat tgaggcttta ttatatattt tgtctgaaac tgatgattgt
     1141 attgtcttaa tataaattaa tgttcaaatc tgtgcttaaa tcattaccat ttacacgtta
     1201 tcttttagtc tagttctaga acttgaaagc ggattttgtt aaaatgaatg agtattgtat
     1261 aacgaaaaaa taaataggag aatggttatg aaaacaacga ttaaacaagc gaaagatcat
     1321 ttaaaccaag acgttacaat tggtgcttgg ctaacaaata aacgttcaag tggtaaaatc
     1381 gcctttttac aattacgtga tggaacaggc tttatgcaag gcgtagtagt taaatcagaa
     1441 gttgatgaag aggtattcaa acttgcgaaa gaaattactc aagaatcatc tctatacgtt
     1501 acaggcacaa ttacagaaga taatcgttct gacttaggat acgaaatgca agtgaaatca
     1561 attgaagtta tttcagaagc gcatgactat ccgattacac ctaaaaatca tggtacagaa
     1621 ttcttaatgg atcaccgtca tttatggtta cgttctaaaa aacaacatgc tgtaatgaaa
     1681 attagaaatg aagttattcg tgcaacgtat gaatttttca acaaagatgg atttacaaag
     1741 gttgatccac caattttgac agcaagtgcg ccagaaggta caagtgaatt attccatact
     1801 aaatactttg atcaagatgc gtttttatct caaagtggtc agttatactt agaagctgca
     1861 gcaatggcac acggaaaagt attttcattt ggtccaactt tcagagctga aaaatcaaaa
     1921 acacgtagac acttgatcga gttctggatg attgaagggg aaatggcttt cacaaatcat
     1981 gctgaaagtt tagaaattca agaacaatat gtaacacatg ttgtaaaatc agttttagaa
     2041 aattgtaaac tagagttgaa aattttagag cgtgatacat caaaacttga aaaagttgcg
     2101 acaccattcc ctagaatttc atatgatgat gcaattgaat tcttaaaatc agaaggcttt
     2161 gatgatattg aatggggcga agattttggt gcgccacatg aaacagccat tgctaatcat
     2221 tatgatttac cggtgtttat tactaattat ccaactaaaa ttaagccttt ctatatgcaa
     2281 ccaaatcctg aaaatgaaga aactgtatta tgtgcagact taattgcacc tgaaggatac
     2341 ggtgaaatta ttggtggatc tgaacgtgtg gatgacttag aattgttaga acaacgcgtt
     2401 aaagagcatg gattagacga agaagcatat agttactact tagacttacg tcgttatggt
     2461 agtgtgccac actgtggatt tggtttaggt ttagaacgta cagtagcatg gatttctggt
     2521 gttgaacacg ttcgtgaaac agcgccattc ccaagattat taaaccgttt atatccataa
     2581 gttttagggt cgttacttaa aatatatatt atgataataa aaagaatcgt ccagcctata
     2641 ttaatagctg ggcgatttat gcttggaaaa tgaagattga taacggctta tattaatcat
     2701 gaaaaaactt ttgtttagta actatatttg gaaaataaat agttcatctg attataaata
     2761 aagttcattg ttaaaatcta gtgttaaaaa atactgttat ttaaaatttg tttgctacta
     2821 gttaaatata ttaagcattt tagtcgaaa
//
LOCUS       DAHHYJ010000197         2802 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000197 DAHHYJ010000000
VERSION     DAHHYJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.201"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            182..1207
                     /locus_tag="NBK22_002510"
     CDS             182..1207
                     /locus_tag="NBK22_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCD2415665.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFIN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
     gene            complement(1768..1962)
                     /locus_tag="NBK22_002511"
     CDS             complement(1768..1962)
                     /locus_tag="NBK22_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415666.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            complement(1973..2620)
                     /locus_tag="NBK22_002512"
                     /pseudo
     CDS             complement(1973..2620)
                     /locus_tag="NBK22_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065796.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
BASE COUNT          922 a          394 c          432 g         1054 t
ORIGIN      
        1 tcgctatgat taaaaattaa ttaattttct taatgtattg tttgatgtat aatatattta
       61 ttcaacttcg cgataatgcg atacaaactt taacaattca caatacattt taaatatcga
      121 actagttttg aaattagttt atcttattaa aatgctgata ttagttttag ggggaaatgt
      181 agtgatctca tttgaaaatg attatttaga aggtgctcat gaaaaagtat taaaacgatt
      241 agtagataca aatcttgtac aagcttcggg atatggcttt gatcaattca cagctcaggc
      301 aattgaaaag attaaagata caattgattg tccaaatgct actatccgtt ttttagtagg
      361 tggaacgcaa accaatcagg ttgttattaa ttcaatgtta gaaagttacg aaggggttat
      421 ttcagcagac actggtcatg ttgctgtgca tgagggcggt gcaattgaat atagtggaca
      481 taaagtaatt actataccct ctaaggaagg aaaagttagt gcttcagatg ttgaaactta
      541 catggagaca tttaaaagtg attttaaaaa ggaccatatg gtattcccag gaatggtata
      601 tatttctcat cctacagaat atggcacatt atactctaaa tcagaattgg aagagttatg
      661 caaagtatgt aagcaatatc agcttccatt atttatggat ggtgcacgat tagggtatgg
      721 attgatgagt gatcaatcag atatgactat taaagacatc gctaaatatt gtgatgtatt
      781 ttatataggt ggtacgaaga taggtgcatt atgcggagaa gcaattgttt ttacgaagaa
      841 taatgaaccg aaacaattta ccactcgaat caaacatcac ggtgcacttt tagcaaaagg
      901 acggttaaca ggcatacaat ttttagaatt atttacggat aatttatatt ttaatataag
      961 cagacatgct atcgaaatgg caaataagat gaaagacggg tttataaata aaggctatcg
     1021 tctttacttt gattctccaa ccaatcaaca attttttatt ttaagtaacg agaaaatagc
     1081 agagttagaa caaaaagtta aatttgctgt ttgggaaaaa tacgatgatc aacatcgtgt
     1141 tgttagattc gcaaccagct gggcgacaac cgaagaaaat ttaaataaac tattagaatt
     1201 aatctaataa tgctgttata tttcgtctgg caaatcattt attaattaag ccagttcagt
     1261 tgagtagttg tgtttattga tttatatatg aaactactca tttgaagccg ttgatgttat
     1321 aaaattacat cattccaaaa caagatacta cagattattg agtatcttgt tttattttat
     1381 atatttaaaa atgttaattt gtgctgtatt tactttaatt tgacgagatt tgaaaagtat
     1441 tatgatggtt gtagttattt tatgaaagtg atgtaatatc atttgaatta ctaaatctat
     1501 tttggtagga atatcgatgt ttagtttaaa tggaatgcac tatttaagtt tttaatatgg
     1561 agatgtttgt gacatttcga tgattaagat ttttataggt gtgcatcttt acaaataaac
     1621 tttgtgttta aaattgaact taggaaatcg ataggtttag atgaagattt tgttgaaggt
     1681 atgtgtcttg tatccttagt tgttataaaa gcgcaaaaaa tggcatcact ttatctttat
     1741 ctgtgtagaa aggatgctat ttagaaatta ttttaataat ctaatacttt ttatctcagt
     1801 ttcatcaaac gaataaacag caaaatcagt ctgtatatct acaacaagat ttcctgtttc
     1861 actctcaaat tcattttcat agtcagttac aaacccttcg tattcttcat tattcaaaag
     1921 cgtgataata acatctttac gatatgcatc ttcaattctc aatacttttc acctacttat
     1981 caatataagg tactatatga gcacctgtct tcgaataatg cacttttcct tttctggttt
     2041 caatatacac attatcgata tgtacttttc caataatttg attaaaatta ataatctctt
     2101 tcatatcaaa tagctcatca cttaatatta gattgcctgt tgacattttt tctcttaaca
     2161 attcattcaa taaatctata gaaagtattg tatagctagg caatttctta ttatttaaaa
     2221 tggctctttt tttattttca ttatatagat gatgacctaa catatgtcca ttttgttttt
     2281 cacaatttat ttctattttc attttacaac tttcaatcat ttctttcatc tggtttttag
     2341 ctttttcttg taatttagct tcttttactt ctacctgata tttaccttca cgctctttaa
     2401 agaacttgtc tcgccaactg ccaacatgtg gcactgtggt acttctacac taaggatgca
     2461 taggtggcgc attcacacct ggtatcatat ctttaacttt aaatattttt ccgttgagtg
     2521 aatgacataa tttagatgtt ttactatcta ttttggcaac atatttatat tcgccatctt
     2581 caccaagttc ttttaaatat gttaactttt gtgattctgc attttcagta aatagttaaa
     2641 aaagcgtata aaaatagcac caccttctct ttatctgtgt agaaaggatg ctattgatat
     2701 ttagactttg attgattaat ttctttttct aattcttctt tcttcgataa ttcatccata
     2761 tgcttaaaaa aatcttctat agaaatgtaa ccatctaatt gt
//
LOCUS       DAHHYJ010000198         2801 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.202, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000198 DAHHYJ010000000
VERSION     DAHHYJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.202"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(168..1016)
                     /gene="dat"
                     /locus_tag="NBK22_002513"
     CDS             complement(168..1016)
                     /gene="dat"
                     /locus_tag="NBK22_002513"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCD2415667.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     ISFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
     gene            complement(1020..2429)
                     /gene="sapep"
                     /locus_tag="NBK22_002514"
     CDS             complement(1020..2429)
                     /gene="sapep"
                     /locus_tag="NBK22_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCD2415668.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLAEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
BASE COUNT          912 a          553 c          373 g          963 t
ORIGIN      
        1 aatttttatc attatatttc aactgaaaat gagaaacaaa atggcacttt ttactaatat
       61 gtgttttcta aacaacactt ttaagcttcg ttttaaatta taacataatt cacttacgaa
      121 agttgataaa tttaagtaat ttaatctaaa aatatgatga aagaatttta aatactgtgt
      181 gactctatat acttttcaaa tccttcttgt agttgacgtg taattgggcc aactttacca
      241 tcattaactg gttcaccatc taatttaata acaggtgtaa cctcagctga agtacttgaa
      301 acaataactt catctgcgtt tttcaagaaa tctacagtaa acgtttcttc tttaaatgaa
      361 atgttatagt cttcggcaat ttttttaatt acaattcgtg taataccatt aagaatatag
      421 ttgttaatcg gatgtgtata aatcacaccg tctttaattg cataagcatt acttgaagat
      481 ccttcagtta cagtttcacc tcgatgttga attgcttcaa ctgcattata tttcacagca
      541 tattcttttg ctaatacatt tcctaataag ttcaagcttt taatgtcgca acgtaaccat
      601 cggatatctt caacggtaac accattcaca ccattttcta aatgatcata aggacgatca
      661 taactctttg tataagcaac aattgctggt tctacttcag gtgtcgggaa gctatgattc
      721 ctttcagcta caccacgcgt tgcttgaata taaattgccc cagtttcaat ttgattcata
      781 tcaactaatt tacgagatag ttcaattaat tcttctacag aataatttaa atctaaacca
      841 atctcattgg cactacgtaa aaatctttca taatgttctg ttactgtaaa taacttacca
      901 ttatatactc gaatgtattc ataaatacca tcgccaaata cgtatcctct gtcgttgtat
      961 gaaacctttg cttcacttgg acttacaaac tcaccattta aaaaaatttt ttccatatat
     1021 tattcctcca cgcataatga ataaattgct tctaagtaaa tactagttgc gttaaataac
     1081 tgttttttag tgatatattc atttttctga tgcattaaat cttcagaatc actaaacatt
     1141 gcgccaaatg ctacaccctt gtctaagttt ctcgcataag taccgccacc tatagtataa
     1201 ggttcagtca tatcatttgt ttgatttcta tatgcagtaa ctaacttttg tacaaaagga
     1261 tcatttttat caacataatg tggtggttgg actttaccta atttcacttc aaagccatat
     1321 tgttgaatct catttgcaaa acgatccata gctttttcaa attcaaatcc ttctgggtag
     1381 cgtaagttga taccgaaaag acctgcgttt tcattatcat atgtaataac accaatgtta
     1441 gttgtcacgt cacccatgac atctgtatgg aatttcattc ccatcttttc accaaaatct
     1501 gaattaaata agtagcgatt actaaatgct acaaacgctt gtgcattatt atcaagattt
     1561 aatgatgcta agaattttag taagtaaaga cccgcattca caccgataga tggatccata
     1621 ccatgaaccg ctttaccttc aactgttaaa actagaatgc cactatcaac agtactatca
     1681 ccttgtaaat gattttgttc taaaaagtac tcaaagtctt gaataacatc tgtcatattt
     1741 tctttaacaa gcactcttgc ttctgcatga tcaggcacca tgttgtaacg ttcaccagat
     1801 ttaaaagtta ttaattcata atcaggttca tcttgatctt cagcaagctt attttgaact
     1861 aagtcaaatg ttgtaatgcc tttttcacca tgaatacacg gaaattctgc atctggtgca
     1921 aaacctaatg ttggcatttc ttctgtttta aaatagcgat ccgtacattt ccaatcagat
     1981 tcttcatccg taccaataat catatgaata cgtttcttcc aatccacatt catatcttct
     2041 aatatcttaa ttgcataata agcagcaatt gttggacctt tgtcatcaag tgtacctcta
     2101 gctatgatag catcttctgt tacaaccggc tcgaacggat tactatccca tccatcacca
     2161 gcaggaacaa cgtcaacatg acataagata cctaatacgt catttccttt acctgcctca
     2221 attcttcctg caatatgatc cacatcatgt gttgtaaatc catctctatg tgcaatttca
     2281 tacatgtagt ctaatgcctt acgaggacct ggaccaactg gtgcgtcttc tgatgctttt
     2341 gcatcatctc tcacactttc aattgctaat aatcctttta agtcattaat gatttgatct
     2401 tcgtattgtt gaactttttc tttccacatt cgaaatcgac ttcctttttt ctataagtta
     2461 aattctattt tacatgaaat gatataaaaa ctacaataag atgtcagaaa ataataaaaa
     2521 ggaacaaaac gatgctatcg atatgacaca aatcataaat agctgctttg ttcctttttt
     2581 aatttatata tttaaaatac acatattcaa gagctcgaga tataagtcaa tgtactaggc
     2641 acacaattta atattgacag taattaaccg aacgaaaatg agccccggag ccccccaaca
     2701 cagagaattt cgaaaagaaa ttctacagac aatgcaagtt ggggtaaggg ccccaacaca
     2761 gagaatttcg aaaagaaatt ctacagacaa tgcaagttgg c
//
LOCUS       DAHHYJ010000199         2757 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000199 DAHHYJ010000000
VERSION     DAHHYJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.203"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            180..905
                     /locus_tag="NBK22_002515"
     CDS             180..905
                     /locus_tag="NBK22_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCD2415669.1"
                     /translation="MKKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISEK
                     ETNINELIKYYTQPHLSLSNKWLWQKPNGSIHATLQTWVWYSHIQVFGSESWGNINQL
                     RNKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKPYRLFLKYSDKQQTIIGGH
                     EFYKGNKPVLTLKELDFRIRQTLIKNKKLYNGEFNKGQIKITADGNNYTIDLSKKLKL
                     TDTNRYVKNPKNAQIEVILEKSN"
     gene            1000..1725
                     /locus_tag="NBK22_002516"
     CDS             1000..1725
                     /locus_tag="NBK22_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCD2415670.1"
                     /translation="MNNNITKKIILSTTLLLLGTASTQFPNTPINSSSEAKAYYINQN
                     ETNVNELTKYYSQKYLTFSNSTLWQKDNGTIHATLLQFSWYSHIQVYGPESWGNINQL
                     RNKSVDIFGIKDQETIDSFALSQETFTGGVTPAATSNDKHYKLNVTYKDKAETFTGGF
                     PVYEGNKPVLTLKELDFRIRQTLIKSKKLYNNSYNKGQIKITGADNNYTIDLSKRLPS
                     TDANRYVKKPQNAKIEVILEKSN"
     gene            1833..2549
                     /locus_tag="NBK22_002517"
     CDS             1833..2549
                     /locus_tag="NBK22_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCD2415671.1"
                     /translation="MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQD
                     ETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQL
                     KDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAH
                     KIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGNLKIIGGGNNYTIDLSKRLHS
                     DLANVYVKNPNKITVDVLFD"
BASE COUNT         1079 a          407 c          403 g          868 t
ORIGIN      
        1 tatactccag cctactttat taggtactgt ttctgtccaa aacttaaaaa tgataagttt
       61 tgcttaaata acactactaa ctgtttaagt ttatttaaca tagttttagc ttttatttaa
      121 ttccgaatcg gtgtaatagc ttatatactt tgggtaattc acgcaaagga gattttcata
      181 tgaaaaagaa catcatgaat aaattagttt tatcaacagc attgttactt ttaggaacca
      241 catctacaca acttcctaaa acaccaatca gtttttcatc tgaagcaaaa gcctataata
      301 tcagtgaaaa agagactaat atcaatgagt taataaagta ttatacacag cctcatttat
      361 cactatcaaa taaatggtta tggcaaaagc ccaatggtag cattcatgca acattgcaaa
      421 cgtgggtttg gtatagtcat attcaagtgt ttggatccga gagttgggga aacattaatc
      481 agttaagaaa taaatacgtt gatatatttg gaactaaaga tgaggacaca gttgaaggtt
      541 actggactta tgatgaaaca tttactggtg gtgttacgcc agcagctact tcatctgata
      601 aaccttatag actattttta aaatatagtg ataaacaaca aactatcatc ggtggacatg
      661 aattttacaa aggaaataaa ccagtattaa ctctaaaaga attagacttc cgtattcgtc
      721 aaacattaat aaagaataaa aagttatata acggagaatt taataaaggt caaattaaga
      781 taactgctga tggaaataat tacacgattg atttaagtaa aaagttaaaa ttaactgaca
      841 caaaccgtta tgttaaaaat cctaaaaatg cacaaattga agtcatactc gaaaaatcta
      901 actaacctat taccttttgt aaatgcggat aatttcaatt atctaattaa ccccttttta
      961 taattaaaca ttccaacaat actcaaagga gaaattcgaa tgaacaataa catcacgaaa
     1021 aaaattattt tatcaacaac attgttacta ttaggtacag catctacaca atttcctaat
     1081 acacctatca attcttcatc tgaagcgaaa gcttattata taaatcaaaa cgaaactaac
     1141 gtaaatgagt taactaaata ttactcgcaa aaatatttaa ccttctcaaa cagtacgtta
     1201 tggcaaaaag ataacggtac gattcatgca acgttgttac agttttcttg gtatagtcat
     1261 attcaagttt atggacctga aagttggggc aatatcaacc aattaagaaa taaaagcgtt
     1321 gatatttttg gcataaaaga ccaagaaacc attgattctt ttgcattatc tcaagaaacg
     1381 tttactggtg gtgttactcc tgcagcaaca tctaacgata aacactataa actgaatgta
     1441 acatataaag ataaagcaga aacgtttact ggcggatttc cagtttatga aggcaataag
     1501 cctgttttaa ctttaaaaga attagatttt cgtattcgtc aaacattaat taaaagtaaa
     1561 aaattatata ataattctta taataaagga caaattaaaa taacaggtgc agacaataac
     1621 tacacaatag atttaagtaa aaggttgcca tcaactgatg caaatagata tgttaaaaaa
     1681 cctcaaaatg caaaaattga agttatcctc gaaaaatcaa actaacaata ataatggagt
     1741 taataaaaat aatcgcaaat actatattga cttcgctcac atttaaattt cttattcctc
     1801 gtatcatgat tcctctgaaa ggagatgttc taatgagtaa gaacatcacg aaaaatataa
     1861 ttttaacgac aacattatta ctattaggta ctgtattacc tcaaaatcaa aaaccagtat
     1921 ttagttttta ctctgaagct aaagcttata gtattggtca agatgaaact aacatcaatg
     1981 aattaattaa atattacaca cagcctcatt tttcattttc aaataaatgg ctatatcaat
     2041 atgataatgg aaacatttat gttgaactta agagatattc atggtcagca catatatctt
     2101 tatggggcgc tgaaagttgg ggaaatatta atcagttaaa agatcgttac gtagatgtgt
     2161 ttggactaaa agacaaagat actgatcagt tatggtggtc ttatagagag acatttacag
     2221 gtggcgttac accagccgca aaaccttctg ataaaactta taatcttttt gtgcaataca
     2281 aagataaact acaaacgatt attggtgcac ataaaatata ccaaggcaat aaaccagtat
     2341 taacattgaa agaaatcgat ttccgtgcac gagaagcgtt aataaaaaat aaaatattat
     2401 ataacgaaaa tcgtaataaa ggtaatctta agatcatcgg tggcggtaat aactatacta
     2461 ttgatttaag caaaagatta cattcagatc tagcaaatgt ttatgttaaa aatcctaata
     2521 aaataactgt tgacgtcctc tttgattagt atatgaaggt gacttatact tcatgcactt
     2581 taattccaaa tcagattatt taaatgataa ttttcaaagt gtatgatgta tataataggt
     2641 aaaattttct ataaatttaa atggaattgg gagtaggaat gtgatagaaa tggtatttta
     2701 taaaatttat ttcgttgtca ctccccaact tgcattgtct gtagaatttc tttttga
//
LOCUS       DAHHYJ010000200         2732 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000200 DAHHYJ010000000
VERSION     DAHHYJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.204"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            34..303
                     /gene="rpsN"
                     /locus_tag="NBK22_002518"
     CDS             34..303
                     /gene="rpsN"
                     /locus_tag="NBK22_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCD2415672.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
     gene            460..1437
                     /gene="guaC"
                     /locus_tag="NBK22_002519"
     CDS             460..1437
                     /gene="guaC"
                     /locus_tag="NBK22_002519"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCD2415673.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKNHIPDSFVIAGNVGTPEGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            1459..2475
                     /locus_tag="NBK22_002520"
     CDS             1459..2475
                     /locus_tag="NBK22_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCD2415674.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMISYIKDKVNALYTNQNIITSKSKIKYNTPKSVVRQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
BASE COUNT         1074 a          341 c          490 g          827 t
ORIGIN      
        1 attacgattt gataaaaagt gaggtaacta tatatggcta agaaatctaa aatagcaaaa
       61 gagagaaaaa gagaagagtt agtaaataaa tattacgaat tacgtaaaga gttaaaagca
      121 aaaggtgatt acgaagcgtt aagaaaatta ccaagagatt catcacctac acgtttaact
      181 agaagatgta aagtaactgg aagacctaga ggtgtattac gtaaatttga aatgtctcgt
      241 attgcgttta gagaacatgc gcacaaagga caaattccag gtgttaaaaa atcaagttgg
      301 taaaatcaaa tttcgtactt tagcccattt acaatatcaa taaaacaatg tacagtatat
      361 acgaatgcta taaactgaat gttttctcat attaataagg aaacattcgg tttttaattt
      421 gcatttaaaa ataattagtt tttaaagggg ctatttaaag tgaaaatatt tgattacgaa
      481 gatattcaat taatacctaa taaatgcata gttgaaagta ggtctgaatg tgatacaact
      541 atccaatttg gtccgaaaaa attcaagcta cctgtagttc ctgcaaatat gcaaacagtt
      601 atgaatgaga aattagcgaa atggtttgct gaaaatgatt acttttatat catgcatcgt
      661 tttgatgaag aagcaagaat accttttata aaacatatgc aaaattcagg cttatttgca
      721 tctatttcag ttggtgtaaa gaaagcggaa tttgatttta ttgaaaagtt agctcaagaa
      781 aaattaatcc ccgaatatat tacaatagat attgcgcatg gtcactcaga ttcagtgata
      841 aacatgatta aacatataaa aaaccatata cctgatagtt ttgttattgc tggtaatgtt
      901 ggtacgccag aaggtgttag agaattagaa aatgctggtg ctgatgctac caaagtcggt
      961 ataggtcctg gtagagtttg tattacaaag attaaaacag gttttggtac tggtggttgg
     1021 cagttagcgg cattaaacat atgtagtaaa gcagctcgta aacctttgat tgctgatggt
     1081 ggtataagaa cgcatggcga catcgctaaa tcaattagat ttggtgcatc aatggtcatg
     1141 attggttcat tatttgcggc acacgaagaa tcacctggtg aaactgtaga acttgatggt
     1201 aaacagtata aagaatattt tggtagtgca tctgaatttc aaaaaggcga acataaaaat
     1261 gtagaaggta aaaaaatgtt tgtagaacat aagggttcat taatggatac cttaaaagaa
     1321 atgcaacaag atttacaaag ctcaatttca tatgccggtg gaaaagactt gaaatcatta
     1381 cgtactgtag attatgttat tgttagaaac tctattttca acggtgatag agattaatat
     1441 ttatagtagg tgatgtaaat taaaaaattc atagtaactg ttgttgcgtt tttatcaatt
     1501 attatcattg cgccaataac agaatttaaa ccattcattc atttacaaaa tgaagtaaga
     1561 caatatattg acattcacat caataaagaa acaatttctg cggaaaataa attggatacg
     1621 ccgaagaaac aacaatttgc ctttaacaat atacaaatga acatgtcaaa atcagatgtt
     1681 gagaaaacat taaataaacc aaaaagagtg acatttaatg aatatggtac gaagtggtat
     1741 acgtattatg atgacgatta caataatttt ataatgataa gttacattaa agataaagtt
     1801 aatgcgttat atacaaatca aaatataatc acttcaaaat caaaaattaa atacaataca
     1861 ccgaaatcgg ttgtaaggca aagattaggc gaaccagaaa cagagattgt taaaggtaga
     1921 gtgcgttacg aacaaaataa taaagaatat gatgttttcc ataaaaatca catttatacg
     1981 acggtatttt atgataagca tcgacgtaat aatgtaacag ctgttttaca agtaagtgat
     2041 gctatggaaa atagattaaa agaacaatat ggagcacctt cgaaatcgct tgcagatagt
     2101 tttgaactac aaaattttga tttagttaat gctgaaagaa aacaacatca attatctaca
     2161 ttgaagtatt ctaaacagaa ttctgaaact gcacgtaagc atagtaaaga tatggccaac
     2221 aatcattatt ttgatcatac aaatttaaaa ggtcaatcac catttgatcg attgaaaaaa
     2281 gatggtatta catttaactc agccggagag aatttagcat atggtcaagt tagtagtatc
     2341 tatgcacatc aaggattaat gaattctatt ggtcacagaa aaaacatttt aaatgatacg
     2401 tttaaaatat taggtgttgg tgttgatttt aatgatgaaa aacaaccttt ttggacagaa
     2461 aattatactg gttaatgttt ttggcatgag tttaaagggt aatgtttgtc tgtaaggaag
     2521 tagataaaca tgatgacaac agataaacca aaagatgcgg atatacttga acgcgtcaaa
     2581 gatatactta ataaaaaaga aagaaaaaaa taataatggc tcgctcctgt aaattattac
     2641 gggggcgagt tttaatttgt ttaacatttt gcaggaatct gtaattcaaa atttatatta
     2701 aagaatgaga ttatctaaca attcgcaatt tc
//
LOCUS       DAHHYJ010000201         2730 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000201 DAHHYJ010000000
VERSION     DAHHYJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.205"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            147..2297
                     /locus_tag="NBK22_002521"
     CDS             147..2297
                     /locus_tag="NBK22_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD2415675.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKYNKCLMLKPEPLVGKMVQEVKE
                     IILNYIEHYKNHLTDVIHIDEDKFETPKLFQTVIQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINDIETTYFVEEAFMSVF
                     RQISPNELSNHNNYEVHFVFDLSLMEIRTIYRMILKLHNIMLNVKLGLNITCLFEKPS
                     VFKSLVSQIKRLKFDSLIIDNANLSSPYLMGESDELLLKNILHFKNLKQVINELDIEQ
                     EKLIFLNVENHKLLNNKERDLSNSAPLIYKTLSALYHNFDGFGLNIFDNHHTFNAMHL
                     YDKNGFKTTLGLILEKFIEYVSKPKYENSYYSIFDIENYYCLVIYDWRVIESETIMSN
                     FEDSQVYINFKNNVLNDKYLIVIETLDENSGNINHLISKELRDKYEWNPSLLSKIDNY
                     LKPAIEIKEHNFSDNSLNINVTFNALYIIKIGKK"
     gene            2443..>2730
                     /gene="sarX"
                     /locus_tag="NBK22_002522"
     CDS             2443..>2730
                     /gene="sarX"
                     /locus_tag="NBK22_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarX"
                     /protein_id="HCD2415676.1"
                     /translation="MNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHC
                     KDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYI"
BASE COUNT         1092 a          306 c          411 g          921 t
ORIGIN      
        1 tatataagtt taaaaattaa ccattatata gttttattta tttttgattt ggtattcgta
       61 ttgtgttaaa tttacgttta attaaataag ttaaatatta tagggaatga taaaagtggt
      121 acttaaatat agaaagaggt aaagttatgg caaaatcatg cttgcatata cttactaata
      181 atgaatatgc gacaacgcgt tgccaagatg gcatagtctt attttggcca attgacgggg
      241 aaatcgaact acaaaaattt cgtaaaagta aaataattga agatgatata tatattatta
      301 atcatctgga tgtatttagt attaagaata ataaaaaaac gatcatgttg tatttgagta
      361 gcgattggtt tgcggaatta ggctttactt tctttaatta ccactataca gcaaagttga
      421 ttaaatcatc ctataatttg aaatgtctac tattaaaatt gacatatcga taccttgata
      481 atcagcctct taatgacgct gatattagaa aattacagga tattattaaa atcattgcaa
      541 aagaagcaag tatggataaa aagattgcac aaaatcaata tcgatatgcc tattatggtg
      601 atttgcgtga cgagctcgaa tatatttatc aaaatgtaaa tcaacgattg acattaaaaa
      661 gtgtcgctga taaattattt gtctcaaagt caaatttgtc atcacaattc cacttactta
      721 tgggcatggg ttttaaaaaa tatattgata ctttgaaaat tggtaaatcg attgaaattc
      781 tacttactac tgatagtact attagcaaca taagtgaaca tttaggtttt agtagtagct
      841 ccacttactc taaaatgttt aaaagttata tggatataac accgaatgaa tatcgtaatt
      901 tatcaaaata taataaatgt ttaatgctaa agccagaacc actagtaggc aaaatggtgc
      961 aagaagtaaa agaaatcata ttgaattata ttgaacatta taaaaaccac ctaactgatg
     1021 ttatacatat tgatgaagac aaatttgaaa cacctaaatt gtttcaaacg gttattcaaa
     1081 taaatactta tacagaaatg aaattagttt tcttagaagg aatctttaaa accttattga
     1141 ataagaacag tcaagttgtc tttttcatca tgccatcgat tctaaaaagt aaaaatacca
     1201 tgtccgaaga agaaaaattc acaatcatta aaacaataat tgaaagtgat ctaaagatag
     1261 catttaatat aaatgatatt gaaacaactt attttgttga agaagctttt atgagtgttt
     1321 tcagacaaat atctccaaac gaattaagta atcataataa ttacgaagtg cattttgttt
     1381 ttgatttatc attgatggaa attagaacaa tttatcgaat gatattaaaa ttacataaca
     1441 tcatgttgaa tgtgaaatta ggattgaaca ttacctgttt atttgaaaaa ccttcagttt
     1501 ttaaatcatt agtatcacaa ataaagcgac ttaaattcga ttcgttaata atagataatg
     1561 caaatttaag tagcccttat ttgatggggg aaagtgatga gttactattg aaaaatattt
     1621 tgcattttaa aaatttaaaa caagtaatta atgaattgga tattgaacaa gaaaagctta
     1681 tttttctaaa tgttgaaaat cataaactgc ttaataataa agaacgagat ttaagtaata
     1741 gtgctccatt aatttataag acattaagtg cgctgtatca caactttgat ggctttggat
     1801 taaacatttt tgacaatcat catacattta atgcgatgca tctatatgat aaaaatggat
     1861 ttaaaacaac actaggtctt atattagaaa agtttatcga atatgtctcg aaaccaaaat
     1921 acgaaaacag ttattattct atttttgata tagagaatta ttattgtctt gttatttatg
     1981 attggcgagt gatagagagc gagacaatta tgagtaattt tgaggatagt caagtttata
     2041 taaattttaa aaacaatgtt ttaaacgata aatatctaat tgtaatagaa acattggacg
     2101 aaaatagtgg caacattaat catttgattt ctaaagaatt aagagataaa tatgaatgga
     2161 accctagttt actatctaaa attgacaact accttaaacc agcaatagag attaaagagc
     2221 ataattttag tgataattcc ttgaatatta acgttacttt taatgcgtta tacataatta
     2281 aaataggaaa aaaataacac cttgatatgt attgcaaaat ttaatttgca tttgttggta
     2341 attttgacaa cgtattaaaa acaaatgaga aagacaatgg caaaaaaatt taagataata
     2401 atgacagaag cattgtcttt atatatttgg gggtgcaaca ttttgaatac tgagaaatta
     2461 gaaacattgc ttggcttcta taaacaatat aaagcattat ctgaatatat tgataaaaaa
     2521 tataagttgt cgctaaatga tttagcagtc ttagatttaa cgatgaagca ttgcaaagat
     2581 gaaaaagtac ttatgcaatc atttttaaaa actgcaatgg atgagctaga tttaagtagg
     2641 acaaaattat tagtttctat aagaagacta attgaaaaag aaagacttag taaagttaga
     2701 tcatctaaag atgagcgtaa aatttatatt
//
LOCUS       DAHHYJ010000202         2723 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.206, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000202 DAHHYJ010000000
VERSION     DAHHYJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.206"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..2080
                     /locus_tag="NBK22_002523"
     CDS             <1..2080
                     /locus_tag="NBK22_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HCD2415677.1"
                     /translation="INNALTKEEIEQAKAQLAQALQDIKDLVKAKEDAKNAMKALANA
                     KRDQINSNPDLTPEQKAKALKEIDEAEKRALQNVENAQTIDQLNRGLNLGLDDIRNTH
                     VWEVDEQPAVNEIFEATPEQILVNGELIVHRDDIITEQDILAHINLIDQLSAEVIDTP
                     STATISDSLTAKVEVTLLDGSKVIVNVPVKVVEKELSVVKQQAIESIENAAQQKIDEI
                     NNSVTLTLEQKEAAIAEVNKLKQQAIDYINNAPDVHSVEEIQQQEQAHIEQFNPEQFT
                     IEQAKSNAIKSIEDAIQHMIDEIKARTDLTDKEKQEAIAKLNQLKEQAIQAIQRAQSI
                     DEISEQLEQFKAQMKAANPTAKELAKRKQEAISRIKDFSNEKINSIRNSEIGTTDEKQ
                     AAMNQINEIVLETIRDINNAHTLQQVEAALNNGIARISAVQIVISDRAKQSSSTGNES
                     NSHLTIGYGTANHPFNSSTIGHKKKIDEDDDIDPLHMRHFSNNFGNVIKNAIGVVGIS
                     GLLASFWFFIAKRRRKEDEEEELEIRDNNKDSIKETLDDTKHLPLLFAKRRRKEDEED
                     VTVEEKDSLNNGESLDKVKHTPFFLPKRRRKEDEEDVEVTNENTDEKVLQDNEHSPIL
                     IAKRRKDKEVDVETTTSIESNEDDAPLLLAKKKNQKDNQSKGKKSASKKPSKKLAAKK
                     KKKKSKKNKK"
     gene            complement(2139..2540)
                     /locus_tag="NBK22_002524"
     CDS             complement(2139..2540)
                     /locus_tag="NBK22_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCD2415678.1"
                     /translation="MAKINFDAATKGNPGISTCAIVIKEDEQHYTYTHELGEMDNHTA
                     EWAACIYALKHARELNVSNALLYTDSKLIADSVNAGYVKNAKFKPYFDQLEIFEQDFD
                     LLFVKWIPREQNKEANQHAQQALYKLIKKNK"
BASE COUNT         1080 a          439 c          466 g          738 t
ORIGIN      
        1 cattaacaat gcgctgacta aagaagaaat tgagcaggca aaagcacaac ttgcgcaagc
       61 attacaagac atcaaagatt tagtgaaagc taaagaagat gcgaaaaatg caatgaaagc
      121 cttagctaat gcgaagcgtg atcaaatcaa ttcaaatcca gatttaacac ctgagcaaaa
      181 agcaaaagcg ctcaaagaaa ttgacgaagc tgaaaaacga gcactacaaa acgttgagaa
      241 tgctcaaact atagatcaat taaatcgagg attaaactta ggtttagatg acattagaaa
      301 tacacatgta tgggaggttg atgaacaacc tgctgtaaat gaaatttttg aagcaacacc
      361 tgagcaaatc ctagttaatg gtgaactcat tgtacatcgt gatgacatca ttacagaaca
      421 agatattctt gcacacataa acttaattga tcagctttca gcagaagtta tcgatacacc
      481 atcaactgca acgatttctg atagcttaac agcaaaagtt gaagttacat tgcttgatgg
      541 atcaaaagtg attgttaatg ttcctgtaaa agttgtagaa aaagaattgt cagtagtcaa
      601 acaacaggca attgaatcaa tcgaaaatgc ggcacaacaa aagattgatg aaatcaataa
      661 tagtgtgaca ttaacactgg aacaaaaaga agctgcaatt gcagaagtta ataagcttaa
      721 acaacaagca attgattata ttaacaatgc acctgatgtt cattcagttg aagaaattca
      781 acaacaagaa caagcgcata ttgaacaatt taatccagaa caatttacga ttgaacaagc
      841 aaaatcaaat gcaattaaat cgattgaaga tgcaattcaa catatgattg atgaaatcaa
      901 agctcgtact gatctaacag ataaagagaa gcaagaagct attgctaagt taaatcaatt
      961 aaaagaacaa gcaattcaag cgattcaacg tgcgcaaagc atcgatgaaa taagtgagca
     1021 attggaacaa tttaaagctc aaatgaaagc agctaatcca acagcaaaag aactagctaa
     1081 acgcaagcaa gaagcaatta gtagaattaa agacttttca aatgaaaaaa taaatagtat
     1141 tcgaaatagt gaaattggca caactgatga aaaacaagca gcaatgaatc aaattaacga
     1201 aattgtgctt gaaacaatta gagatattaa taatgcgcat acattacagc aagttgaggc
     1261 tgcattgaac aatggtattg ctcgaatttc agcagtacaa attgtaatat ctgatcgtgc
     1321 taaacaatcg tcaagtactg gaaatgaatc taatagccat ttaacaattg gttatggaac
     1381 tgcaaatcat ccatttaaca gttcgactat tggacataaa aagaaaattg atgaagatga
     1441 tgacattgat ccacttcata tgcgtcactt tagtaataat ttcggtaatg ttattaaaaa
     1501 cgctattggt gtggtgggta tctctggctt actagctagt ttctggttct tcattgccaa
     1561 acgtcgtcgt aaagaagatg aagaggaaga attagaaata agagataata ataaagattc
     1621 aataaaagag actttagacg atacaaaaca tttaccactt ttatttgcga aacgtcgcag
     1681 aaaagaagat gaagaagatg ttactgttga agaaaaagat tcgctaaata atggcgagtc
     1741 actcgataaa gttaaacata cgccgttctt cttaccaaaa cgtcgtcgta aagaagatga
     1801 agaagatgtg gaagttacaa atgaaaacac agatgaaaaa gtgttgcaag ataacgaaca
     1861 ttcaccaatc ttaatcgcaa aacgacgcaa agataaagag gtagatgttg aaacaacaac
     1921 tagtattgaa tctaatgaag atgacgcacc tttattattg gctaaaaaga aaaatcaaaa
     1981 agataaccaa tccaaaggga aaaagtcggc atcaaaaaaa ccttctaaaa agctagcagc
     2041 taaaaagaag aaaaagaaat ctaaaaaaaa taaaaagtaa tttgtttctt cgataaaaag
     2101 aggagcaccg attgaaatta tgtcagtcgg tgctcctttt atttgttctt tttaattaat
     2161 ttgtacaatg cttgctgtgc gtgttgattc gcttctttgt tttgttctct cggaatccat
     2221 ttaacaaata ataaatcaaa atcttgttca aatatttcca attgatcaaa ataaggttta
     2281 aattttgcat tttttacata accagcgtta acactatccg caattaactt agagtcagtg
     2341 tacaacaacg cattgctaac gtttaattca cgggcgtgtt tcaatgcata gatacaagca
     2401 gcccattctg cagtgtggtt atccatttcg cctaactcat gtgtatatgt ataatgctgc
     2461 tcatcttctt tgattacaat ggcacatgta cttatgcctg gatttccttt cgtcgcagca
     2521 tcaaaattta ttttcgccat aataaaccta ctttctattc aatacttagt taaagttact
     2581 attactgtaa tacaaaatat gttgggtaat ccattaaaaa acacgcatca cttaaataag
     2641 taacacgtgt ttaaaatact cgctgattca aagatgattt tctaatacgt agtactgtaa
     2701 tatacttcct aaaaaaatca tct
//
LOCUS       DAHHYJ010000203         2705 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000203 DAHHYJ010000000
VERSION     DAHHYJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.207"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            55..738
                     /gene="isdC"
                     /locus_tag="NBK22_002525"
     CDS             55..738
                     /gene="isdC"
                     /locus_tag="NBK22_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCD2415679.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            738..1814
                     /gene="isdD"
                     /locus_tag="NBK22_002526"
     CDS             738..1814
                     /gene="isdD"
                     /locus_tag="NBK22_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCD2415680.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELLIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQNKENNQDQLKKDIDDKKDSQKSDTKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENSPPTIKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            1811..2689
                     /gene="isdE"
                     /locus_tag="NBK22_002527"
     CDS             1811..2689
                     /gene="isdE"
                     /locus_tag="NBK22_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCD2415681.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
BASE COUNT         1144 a          355 c          457 g          749 t
ORIGIN      
        1 tatttatgat tttactaaag acttaaaagc aatcataaaa aggaggatta tgttttgaaa
       61 aatattttaa aagtttttaa tacaacgatt ttagcgttaa ttatcatcat cgcgacattc
      121 agtaattctg caaatgccgc agatagcggt actttgaatt atgaggttta caaatacaat
      181 accaatgaca cgtcaattgc taatgactat tttaataaac cggcaaagta cattaagaaa
      241 aatggtaaat tgtatgttca aataactgtc aaccacagtc attggattac tggaatgagt
      301 atcgaaggac ataaagaaaa tattattagt aaaaacactg ccaaagatga acgcacttct
      361 gaatttgaag taagtaagtt gaacggtaaa atagatggaa aaattgacgt ttatatcgat
      421 gaaaaagtaa atggtaagcc attcaaatat gaccatcatt acaacattac atataaattt
      481 aatggaccaa ctgatgtagc aggtgctaat gcaccaggta aagatgataa aaattctgct
      541 tcaggtagtg acaaaggatc tgatggaacg actactggtc aaagtgaatc taacagttcg
      601 aataaagaca aagtagaaaa tccacaaaca aatgctggta cacctgcata tatatatgca
      661 ataccagttg catccttagc attattaatc gcaatcacat tgtttgttag aaaaaaatct
      721 aaaggcaatg tggaataatg agaaatgtta aacaaattgc tacaaaatct attatagcta
      781 ttattagctt aggtatactt acatatacaa caatgattgg tagcgtgttg gctgatgaga
      841 taaaatatcc atcagccaaa tttaatcaac ctgaagcaaa agataaaaca gaattaacta
      901 catcaatttt tgatgaaaag ataaaagaga ataaagcgtt agagttacta atttttaatc
      961 aagaaaataa aaatgtaact gaggaacaac aactagttga cgaaaaggcg caattgattt
     1021 cagatatgac tggtaaaatt tacttgcaag taaagctaaa aggtcaaatt gataaagaac
     1081 aacttgtttt tcaaaatgac aaaaatgaag aatttccttt tgttataaaa gatgaaaagg
     1141 atgacacaat agtaagaatt ttaattgaac agcatatgga taaaatcaat atgcatgtta
     1201 aaacgttggc tgaaaagaaa aatctagata acaaagaaat ggtgtattct attcatttta
     1261 aagagaaaaa agttcaacat gatgatgcaa aagaagtgcc ttcaaaacat caaaataaag
     1321 aaaataatca agatcagctt aaaaaagata ttgatgacaa aaaagatagt caaaaatcag
     1381 atactaagga aagacgtact agccttttta ctgaaaaagg attaaatgat attcctgtac
     1441 aaaaagataa agtgcaacaa gacagtaata aaaagattga aaatgagcga cctaaagcat
     1501 caggtacatt aaaagttgaa aatagccctc caacaataaa aaaggttgaa aataatcaca
     1561 aagagcaacc gaaacataaa gatgaaaaat caaaaaagga aaagaaaaaa gtagttgaaa
     1621 aagaaaaagc gttaccagct tttaatagag atgatgatag caagaatagt agtcaattat
     1681 ctagtgatat taaagaactt gatgaaccaa atcataaaaa gcaatatatg ttatttgcag
     1741 ctggcattgt gttagcaact attttactta tttcggcaca tttatacagc agaaagagag
     1801 gtaaccaagt ttgagaatca taaagtattt aaccatttta gtgataagcg tcgttatctt
     1861 aaccagctgt caatcttcca gttctcaaga atcaactaaa tccggcgaat tcagaatcgt
     1921 accaacaact gttgcattga caatgacatt ggacaaattg gatttaccaa ttgtcggcaa
     1981 acccacgtca tataagacat tgcctaatcg ttataaagat gtacctgaaa ttggtcaacc
     2041 aatggagccg aatgttgaag ctgttaaaaa attaaaacca acacatgttt tgagtgtgtc
     2101 aacgattaaa gatgaaatgc aaccatttta caaacaatta aatatgaaag gctactttta
     2161 tgattttgat agtttaaaag ggatgcaaaa gtcgattaca caattaggtg atcaatttaa
     2221 tcgtaaagca caagcaaaag aattaaatga ccatttaaat tctgtaaagc aaaaaattga
     2281 aaataaagca gctaaacaaa agaaacatcc caaagtatta atattaatgg gtgtacctgg
     2341 tagctattta gtagcaactg ataaatcata tattggtgat ttagttaaaa tagcaggtgg
     2401 agaaaatgtt attaaagtga aagatcgtca atatatttcg tctaatactg aaaatttgtt
     2461 gaatatcaat ccagatatta ttttaagatt accacacgga atgcctgaag aagttaagaa
     2521 aatgtttcaa aaagaattta aacagaatga tatttggaaa cattttaaag ctgtgaaaaa
     2581 taatcatgtt tatgacttag aggaagtgcc attcggtatt acagcaaatg ttgatgctga
     2641 taaggcaatg actcaattat atgatttatt ttataaggat aaaaaatagt gagttgatat
     2701 gaaga
//
LOCUS       DAHHYJ010000204         2674 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000204 DAHHYJ010000000
VERSION     DAHHYJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.208"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            89..427
                     /locus_tag="NBK22_002528"
     CDS             89..427
                     /locus_tag="NBK22_002528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415682.1"
                     /translation="MLSRIDKIIVHSFGLFVNYTTINLIQLLNVLFINSNALTISYVL
                     FSSTMIWNLVPILNSDGYKIMLAALCLDEYKNYTKNHWIILIFQLIGVTIALNTLVHW
                     IIYWSKYLYT"
     gene            439..1074
                     /locus_tag="NBK22_002529"
     CDS             439..1074
                     /locus_tag="NBK22_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2415683.1"
                     /translation="MKLNNYSLKVKKKQLVENCNLNFYLGQINHIVGKNGVGKSQLAK
                     DFLLNNSGNIPKSISQNVSLISSSSNIPNDITKDFLLSLLKTKFKNNLQTFDKIYKIL
                     NIEVIPSNILLKNLSDGQKQKLKLLSFLLEDHDLIILDEITNALDKKTVNEIYDFLND
                     FIQNHQTKTIINITHNLSDLSALPGKYFIFKNLQIEEYQSKEEVINDYINL"
     gene            1080..1811
                     /locus_tag="NBK22_002530"
     CDS             1080..1811
                     /locus_tag="NBK22_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCD2415684.1"
                     /translation="MSTLKLEFKKSISNKIIFTFGVLFSFLFLLGYFLPIGIDKVKNL
                     SYGQFFFSSYTVATEFGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFIECLIYIILGITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFICPTEIINTVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(1973..2593)
                     /gene="srtA"
                     /locus_tag="NBK22_002531"
     CDS             complement(1973..2593)
                     /gene="srtA"
                     /locus_tag="NBK22_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCD2415685.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDNKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWEKRKIFVATEVK"
BASE COUNT          883 a          390 c          346 g         1055 t
ORIGIN      
        1 atattttgat aaaaaaattg ataaagttgg ttttaagtta aattatttta tttttccttc
       61 tttttacgtt caaatgaacg aaacttacat gctttctagg attgataaaa taattgttca
      121 ttcttttgga ttatttgtaa attacactac aattaatttg attcaattac ttaatgtatt
      181 atttatcaat agcaatgcgc ttacaatttc atatgtactg ttttcatcaa caatgatttg
      241 gaatttagtg ccaattttga actctgacgg ctataaaatt atgttagctg ctttatgttt
      301 agatgaatat aaaaattaca ctaaaaatca ttggattatt ttaatatttc aattaattgg
      361 cgttactatt gcgttaaata ctttagtaca ttggattata tactggagta aatatcttta
      421 tacataggag ttgtatacat gaaactaaat aactattctt taaaagttaa aaaaaaacag
      481 ttagttgaga attgtaattt aaatttctat cttggtcaga tcaatcatat tgttggtaaa
      541 aatggtgttg gaaagtctca attagctaaa gatttcttac taaataatag tgggaatatc
      601 cctaagtcca tttctcaaaa tgtaagctta atatctagtt catcgaatat tcctaatgat
      661 ataactaaag actttttatt atcattgtta aaaacaaaat tcaaaaacaa cctacaaacc
      721 ttcgacaaaa tttataaaat tctaaacatc gaagtaatac cgtctaacat actactaaaa
      781 aacttgagtg atggtcaaaa acaaaagctt aaattattaa gcttcttatt ggaagaccat
      841 gatttaatca tattagatga aattacaaac gcattagata agaagacagt taatgaaatt
      901 tatgattttt taaatgattt tatacaaaat catcaaacta aaactattat caacattaca
      961 cataatttat ccgatttaag tgctttgccg ggtaaatact ttatttttaa aaatctacaa
     1021 atagaagaat atcaatctaa agaagaagtc ataaatgatt acattaattt ataattgagg
     1081 tgtccacttt gaaattagaa tttaaaaaaa gtatttctaa taaaattatt tttacatttg
     1141 gtgtattatt ttcattcctt ttcttacttg gttatttttt accaataggt attgataaag
     1201 ttaaaaattt aagctatggt cagtttttct ttagttctta tacagttgcc actgaatttg
     1261 gctttttatt attttcattt gttattgcat attttattaa taaagagtat tcgaataaaa
     1321 atatattatt ttacaagctg attggcgata atatatttac cttcttctat aaaaaagtag
     1381 cagttctatt cattgaatgt ttaatttata ttattttagg catcacaatt atttcaataa
     1441 tatattcgga tttctcacat tacttactat taataatctt attttcactc gttatcttac
     1501 aatatatctt agttgttgga acgattagta tggtttcacc taacatttta atcagcttag
     1561 gtatcagcat tgtatattgg attggttcag tcatcttagt tgccattaat aaaaatatat
     1621 ttggtatcgt tgctccgttt gaggcgagca acacgatgta tcgtgccgta gaaaaaatat
     1681 tgaataacga gtcaacattt atatgtccta ctgaaattat aaatacagta tcattcttcg
     1741 ttcttctatt tatagttaat actattgtct tattactaag tagaaagaga tggttgaaaa
     1801 tcggtatgta gtacataaaa aagagcggca tccattgtcg ctccttttta ttatgtattt
     1861 ttacaatctc catcaatgac acgtcgtcat tggtgcctca caccaaacaa aaaaacagtc
     1921 tcgcaatcaa gactgttttc cactcaatat attcatccat tagcgtaatg gattatttga
     1981 cttctgtagc tacaaagatt ttacgttttt cccaaacgcc tgtcttttca ttgtaatcat
     2041 cacaagtaat taatgttaat tgtttatctt tacctttttg ttcatctaga acttctacat
     2101 ctgttggctt aacatctctt atacttgtca ttttatactt acgtgtttca ttaccaactt
     2161 taaagtacac catactacct tttttggctg ctttaagatt tgtaaattga tagttcggac
     2221 ggtcaatgaa agtgtgtcct gcaattgaaa tattttgatc atctagtgat tcattttctt
     2281 ctgcaaagct tacacctcta tttaattgtt caggtgttgc tggtcctgga tatactggtt
     2341 ctttaatatc agcatctgga atttcaatat agcctgccac ttttgattta tctttcggaa
     2401 tttgaggttt agcttgctgc ttattgtctt tactcgcctg ttcttttaca tttttatcat
     2461 attgttcaat cttttcatct ttatctttat cgtgaagata attatcgata tgtggtttag
     2521 caaacaaata tgctgccact aggataagta ctacaccagc gattgtcatt aatcgatttg
     2581 tccatttttt catacgttaa ggctcctttt atacatttca caatgcctat taacatttca
     2641 tatagtatac cattaattat aaaatgactc atag
//
LOCUS       DAHHYJ010000205         2624 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000205 DAHHYJ010000000
VERSION     DAHHYJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.209"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            15..497
                     /locus_tag="NBK22_002532"
     CDS             15..497
                     /locus_tag="NBK22_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415686.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            499..666
                     /locus_tag="NBK22_002533"
     CDS             499..666
                     /locus_tag="NBK22_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCD2415687.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(806..>2624)
                     /gene="typA"
                     /locus_tag="NBK22_002534"
     CDS             complement(806..>2624)
                     /gene="typA"
                     /locus_tag="NBK22_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCD2415688.1"
                     /translation="IAIIAHVDHGKTTLVDELLKQSGIFRENEHVDERAMDSNDIERE
                     RGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVVDAYEGTMPQT
                     RFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQLEFPVVYASA
                     VNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYNDYVGRIGIGR
                     VFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDLIAVSGMEDIN
                     VGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQIQERLNQQLET
                     DVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVIIKEIDGVMCEP
                     FERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARGMIGYTTEFMS
                     MTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLEDRGVNFMEPG
                     TEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRILTLEEALQFIN
                     DDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
BASE COUNT          838 a          505 c          394 g          887 t
ORIGIN      
        1 tagggagtgg gaaaatgata caaattaaag gagccgtaaa gtttcctata acattagata
       61 gtacgacttg gattttcgat gatagaaaag ttagcatcga agatttagaa aaaggtgtat
      121 ttgatggttc aaaaccaatc gactttgaag ataatcatga atggaatcgt gctatcctag
      181 aaggacaaac taatccgcct actttaaatt cagaaataaa atataaaaaa cgtgccatgt
      241 tagaaggttc atttgtcatt aatatgacac catttttcaa acattccgaa ccacatgact
      301 ctgcaactac tattcgatta tcaaatgagc atgatcattt agatataccg atagatctat
      361 taccctattt atttttccaa tttgcaaaag acggtaaacg actttacagc gataatggtg
      421 tagatagttt tatttacaat caagaagatg gttattcata tcgaatcaac catgtaactc
      481 atattgaggt gatttaattt gagacaagtg caatgcatta tctgtgatgc aaaagtattt
      541 atagatgagc gcacaactga gtctaaacga cttaaaaaca atcctattcg aacatttatg
      601 tgcgatgatt gtaaaagtcg attagacaca cctaaacaac gtgcgcaaca ctatccgctc
      661 gattaatatc tgaataaaca agttagaaat ttgaaaatgt gaaagtaaca ggaaataata
      721 gcatttaaac actttttcaa atattatatc ccaaaagaga agctaagcaa ctttcatcgc
      781 tcagcttctc ttttattatg ttaaattatt cgttttcttg catcatttgt ttgatacgct
      841 ttgcttcttt ttcacgaaca tttttgttta aaattttctt tcttaaacgt atactttctg
      901 gtgtaacctc aacaagttca tcatcattaa tgaattgtaa cgcttcttcc aatgttagaa
      961 tacgcggtct attcattgtt tgtgtttggt ctttcgttgc agaacgtacg ttagtttgat
     1021 gttttgtttt agtgatgtta acagttaaat cattttcacg attatgttca ccaacaatca
     1081 taccttcata aacttcagta ccaggttcca tgaagtttac acctctatct tcaagtccca
     1141 aaatggcata agtacttgca gaaccttgat ccattgaaat taatgcacca ttacgacgac
     1201 cgccaatttg tgctttaata cgtggtctaa attcttcaaa tgtatggtta ataataccgt
     1261 aacctcttgt cattgacata aattcagtcg tataaccaat cataccacga gccggtacat
     1321 taaagattaa acgtgtaagt ccattatcag ttgtagtcat atcaaccatt tcacctttac
     1381 gtgcacctaa tgattcaata acagcacctg cattttcttg tggcacttca cattgcacac
     1441 gttcaaatgg ttcacacatt acaccatcta tttctttaat aattacttgt ggttttgaaa
     1501 cttgtaattc ataaccttca cgacgcatat tttcaataag gattgataaa tgcaattcac
     1561 cgcgaccagc aactacccat gtatctggag aatctgtgtt agaaactttc aaagatacat
     1621 ctgtttctaa ttgttgattt aaacgttctt gaatttgacg tgctgttaca aagtcacctt
     1681 cacggccagc aaatggagaa ttgttaactt taaatgtcat ttcaagagta ggctcatcaa
     1741 tacgtagaac tggcaatgct tcttgatggt catgtggtgt tacagtttca ccaacattaa
     1801 tgtcttccat acctgaaaca gcaattaaat ctccagcttg tgcttcttca atttctaaac
     1861 gttttaatcc aaagtaacca aagattttag ttacacggaa gtttttcact gtaccgtcta
     1921 atttaattag tgatacatta tctccgacac gcattttacc tctgaataca cgaccaatac
     1981 caatacgtcc aacataatca ttgtagtcca acaatgctac ttggaattgt aatggctcat
     2041 cactgttatc aattggagct ggtacataat caataattgt ttcatataat gattgtaaat
     2101 tatcatcttg tttttcagga tctaagctag ctgttccatt tactgctgaa gcataaacaa
     2161 cagggaattc taattgttca tcgtttgctt ctaattcaat aaataaatct aaaacttcat
     2221 ctacaacacc ctctggacgt gctgatggtt tatcaatttt attaacaaca acaacaggtt
     2281 tcaggttttg ttctagcgct tttttaagta caaaacgtgt ttgaggcatt gtaccttcat
     2341 acgcatctac tactaagaca accccatcaa ccattttcat aatacgttct acttctccac
     2401 caaagtctgc atgtcctggt gtatccaaaa tattaatacg tgtaccttta taatcaacag
     2461 ccgtattttt ggctagaatc gtaattccac gctctctttc aatatcgtta gagtccattg
     2521 cacgttcatc gacatgttca ttttctctga atataccaga ttgttttaac aactcatcta
     2581 ctaaagttgt tttaccatgg tcaacgtgag caataattgc tata
//
LOCUS       DAHHYJ010000206         2605 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.210, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000206 DAHHYJ010000000
VERSION     DAHHYJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.210"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..1191
                     /locus_tag="NBK22_002535"
     CDS             <1..1191
                     /locus_tag="NBK22_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCD2415689.1"
                     /translation="VINLWTFLMLLNLNIANMIKGKQHFKTIRFINTVCKSILLVLIA
                     SVMIENTSVIKDLNKIKETEKYWNVLDDYYTIEFAPYHETKQSLIDNMLRSEQLVKAS
                     EAENNAILFKPKGDSVDNDNFSPDEGNVILVNNQFWSIYYKQFQPDIPIKNQKNNVEV
                     IIPQKFHAMRNEINQAYHSWFEFVQNKNNKENKLSIQFINKNDYRIFSFDARDSRHLS
                     FIEAPIIVNVQASDLSNDFYYAMISQGGYLFKNYDALVKNIEKYHLDGEISGITNYKD
                     SVMEMYHENNLKLTVLNFSQIIIAIILIIIILFDVKYYFEQHRKLLVIKKLYGYSTLR
                     ANYQYLLINNIVVVFIGILTNVILHSQYIMMIFATILVVQILLQICSLYYHGRRFNEV
                     IKEF"
     gene            1194..1514
                     /locus_tag="NBK22_002536"
     CDS             1194..1514
                     /locus_tag="NBK22_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD2415690.1"
                     /translation="MKFIIAILLGLIVSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDIPKE
                     VRQE"
     gene            1511..2152
                     /locus_tag="NBK22_002537"
     CDS             1511..2152
                     /locus_tag="NBK22_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2415691.1"
                     /translation="MIELKDLTIQKGNIHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYVYFNGQLEQFKSNFYRDKLGYLFQNYGLIDNLTVNENLDIGLAYK
                     KISKKEKEQLKIRYIEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFDLVDENKVLILATHDMAIANQCDEIIDLEQYSKVASM"
     gene            complement(2240..2530)
                     /locus_tag="NBK22_002538"
     CDS             complement(2240..2530)
                     /locus_tag="NBK22_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415692.1"
                     /translation="MFYACHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLIVLNIIILLFFSLPLLLS"
BASE COUNT         1030 a          316 c          419 g          840 t
ORIGIN      
        1 gttattaatt tatggacttt cttaatgtta ctaaatttaa atattgctaa tatgattaaa
       61 ggtaagcagc actttaaaac aattcgtttt attaatacag tttgtaaaag tattctctta
      121 gtactaatag ctagtgtaat gatagaaaat actagtgtta ttaaagattt gaataaaata
      181 aaggaaactg agaaatattg gaatgtatta gatgattatt acacgattga atttgcacct
      241 tatcacgaaa cgaaacaaag tttgattgat aatatgctgc gatcagaaca attagtaaag
      301 gctagtgaag cagaaaataa tgcgatttta ttcaaaccaa agggtgactc cgttgacaat
      361 gacaactttt cgcctgatga ggggaatgta atattagtaa ataatcagtt ctggtcgatt
      421 tattacaagc agtttcaacc tgatattccg ataaaaaatc aaaaaaataa tgtcgaagta
      481 attattccac aaaagtttca tgcaatgcgt aatgaaatca atcaagcata tcattcatgg
      541 tttgaatttg tacaaaataa aaataataaa gagaataagt tatctataca gtttatcaac
      601 aaaaatgatt atcgaatttt ttcatttgat gcacgagata gtcgccattt gtcatttata
      661 gaggcgccaa tcattgtgaa tgttcaggca tcagatttat cgaatgattt ttattatgcc
      721 atgatcagtc aaggcgggta tttattcaaa aattatgacg cgctagtaaa aaatattgaa
      781 aagtatcatc ttgatgggga aatcagtgga ataaccaatt ataaagatag cgtgatggaa
      841 atgtatcatg aaaacaattt gaaattaaca gtactcaact tttcacaaat cattatcgca
      901 atcattttaa taattattat tttatttgat gtgaaatatt attttgaaca gcatcgaaaa
      961 ttactcgtaa tcaaaaagct atatggttat tcaacattaa gagccaatta ccaatactta
     1021 ttaataaata atatagttgt tgtttttatt ggaatattga cgaatgtaat tttacattct
     1081 cagtatataa tgatgatatt tgcaacgatt cttgtcgttc aaatattatt gcagatttgc
     1141 agcctatact atcatggccg acgttttaat gaagttatca aggagtttta actatgaaat
     1201 ttatcattgc aatattatta ggattaatcg tatccattac tattgctttt acaatcatac
     1261 atcatcctat acttgatcgt tttaatcctt tcttaaaaac ggagtatagt tatgccaaag
     1321 tgccaaaagg tacgcaacaa tatgttaata ttacggctta tagtgaaaga ggggaaaagc
     1381 ttgattataa attaacattt aatggatttt cacctagtag aacgtatgtc gaaataaagc
     1441 ataaagggca atatgtcata tcgatcacat atgttgaaaa agaggatata ccaaaagagg
     1501 taagacaaga atgatagaat taaaagattt aaccatacaa aaaggtaata tacatatttt
     1561 gaagaaactt aatttgaaat ttcaatgtgg aaaatcatat gcacttattg gtaaaagtgg
     1621 atgcggtaaa tctacattat taaatactat tgctggactt gaaaaaacgg ggaaacaata
     1681 tgtctatttt aatggtcaat tagaacaatt taaatctaat ttttacagag ataaattagg
     1741 atatttattt caaaattatg gattaatcga taatttgaca gtaaatgaaa atttagatat
     1801 tggattagca tataaaaaaa taagtaagaa agaaaaagaa caattaaaga tacgttatat
     1861 agaacagttt ggtctgtcaa acagtttaaa aagaaaagtt catacgctaa gtggaggtga
     1921 acaacaacgt gtcgctttaa ttagaatgat gttaaaagat ccgattgtta tgttagctga
     1981 tgaaccaacg ggtgcgttag atcctaaaac aggacagatg attattcaat cattatttga
     2041 tttggtcgat gaaaataaag tgctgatttt agcaacacat gatatggcta ttgcaaatca
     2101 atgtgacgaa ataatagatt tagaacagta tagtaaagta gcatctatgt gatatccaaa
     2161 agataaaatt aaaagccaat caatgggaaa tgttgacttc aacactccgt ttgattggct
     2221 tttataatgt gttactaagt tatgaaagta acaacggtaa actgaaaaaa agcaatataa
     2281 taatatttaa tacgattagt aataaatgta attgattttt cgttttaata tagcaaaaaa
     2341 caatgactaa agataacaac aatatcatga ttgcacctgg gaagaaccat aatgctgaag
     2401 ctgtgtacca aatcattggt aacgccataa taataaaaga tataagacaa gaaaggatac
     2461 caataatatt ataagttcta gtcaaaatta cacctccaat tttcatttta aaatgacatg
     2521 cgtaaaacaa tcattttaaa tacatcatac aataaaatta aattgtgcgg tagaagaatt
     2581 tgaaattaaa ttgaattggc ttgat
//
LOCUS       DAHHYJ010000207         2597 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000207 DAHHYJ010000000
VERSION     DAHHYJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.211"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(390..1598)
                     /locus_tag="NBK22_002539"
     CDS             complement(390..1598)
                     /locus_tag="NBK22_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD2415693.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            complement(1591..1758)
                     /locus_tag="NBK22_002540"
     CDS             complement(1591..1758)
                     /locus_tag="NBK22_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415694.1"
                     /translation="MKKGLINPKLSVLHGRFVTVKGICMVSLMLVILHKWTVFIQHIM
                     NILSGGESIIE"
     gene            complement(1733..2584)
                     /locus_tag="NBK22_002541"
     CDS             complement(1733..2584)
                     /locus_tag="NBK22_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCD2415695.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMALNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVNISESEIIYLTLHIHHF
                     EERINQS"
BASE COUNT          952 a          424 c          324 g          897 t
ORIGIN      
        1 aacacttttg tcatattgat atctgtatta agaagtaaat agaaaatatt tctatctcaa
       61 acagaacaat atggttatct atactgattc atatttaaca taaagaaaat gatgaatcaa
      121 tacttatgaa aaatattatt aattaacagc cgattatgag tgcttcttaa tatattaaaa
      181 ataccactta tttttatttt cgacataata atgaagtact aatcaagtgg caaatactcg
      241 tttcattatg catgtgcttt ataaacggct aaactttcgt aaaaatttat aagctaaaca
      301 tgtgctgata atcataaatg taactgccca acattataag caccataaaa aataccaaca
      361 aacatatagt cattggtatt ttttattact tattcaacta ttttattact ttctttaata
      421 tcatattcac ctacatcatt accatagaaa cgattgtatc gacgtttaaa taattggaat
      481 aaatgagtaa ctaatacttt taaaatagca tatcccggaa tacctaaaat aacgcctaca
      541 ataccaagca atttgcctgc acacagtaaa atgaaaatga ttgtaagtgg atgaatctta
      601 agtgttttac ccatgatatt tggtgaaatg aagtgccctt caacaaattg tactaaagtc
      661 catactactg ctaattttaa gagcatccac ggcgatgtta tagcagctat tacaatagct
      721 ggagaaatcg ctatagtagg ccctaaatat ggtacaacac ttgtaactgc cgcaatacta
      781 gctaatacta agctatattt caacccgata accgaataac cgataaacaa cagtataccg
      841 atacagaatg aaacgataat ttgtccttga atgtatgaac caacttgaac actcattttt
      901 tcaagtagat catgaaaatc ttttcggaat ttcggtggca taatattcgt tgaaaattct
      961 ttgaagtgat gtccatcttt aagcataaag aatagtacaa atggtgttgt gacaatcaca
     1021 acgccaatat tagcaatcgt ttctgcaaac gttgcaattt tagacccaaa gccatctgta
     1081 aattcactta acatagatgg tattttctta ggtaaagaat ttaaccaatc attaatttga
     1141 ctataatacg aagagaaaaa cgtattattt gctattttat caatagaatt aattaatttt
     1201 tctagatatt gcggtgaatt tttaactaaa ctatctactt gcgaaccaat aatagggatt
     1261 aataaattaa caattaacgt aataacacct actacagcta gataaataat agaaatacct
     1321 gcaactcttg gtattctata acgttccatc atatttacga ttgggttaaa tagatagaat
     1381 agaattaaag agacgatgat aggtgctgct atcgtcttaa aaacgatgat aaaaggatca
     1441 aaaacgtatg atactttttc gaaaataaaa ataacaatac ccaacaatac tagcgctatt
     1501 aatgagaaga ctaaatcatt cccaccaaca aacttcatat accgtgattc ggtaaatttt
     1561 aaaaaattct ttcttatatt cttttcattt tcattcaatt atagattcac caccacttaa
     1621 aatattcata atatgttgta taaaaacagt ccacttatgt aaaattacta acatcaatga
     1681 taccatacaa atacccttta cagtaacgaa acgcccgtgt aatacactta atttaggatt
     1741 gattaatcct ttcttcaaaa tgatgaatgt gtaatgttaa atatataatt tcagactcac
     1801 tgatattaac atcaaattgt ttttgtatca tagttaaaat tttatatgct gtgttatagc
     1861 aaatcggata gtgattttta atcatagaca caaaatcatc ttgtgcatgt atatattctt
     1921 ttcttcttaa tcggcgaatt aaaaattgta cgtgtcttat aaaacgttgg tattgtaatg
     1981 attccttatc aacagttgtc acaaggtctg attcaatgat atctatacct tttttaataa
     2041 cattattgat caaggtcatc tcatgcatag ataaatcttc tgtattagat gcaatatgta
     2101 atgcaataaa tcctatctca tcttcaggaa aatgtacatc taatgctgca tttaactgat
     2161 caatcacctg tttagcaata tgatatgcat cactatataa ctgcatagtt tccataacaa
     2221 atggattgct aataacttga ttttgtttta agcgtttata agcaaatata atatgatccg
     2281 ttaatgaaac tacaagttgt tttgaatcaa cattcatcgc agtattagaa ataaaattca
     2341 acgaatcaat aattacttgt aatacattat catcagcgat ttcaactaaa cttttataat
     2401 gtgctttttg ttgctcactc tctaatttat aaattttctc tattgtaata gtttggtcgt
     2461 ttaacgccat tccctctttt ttgttaaaac caataccttt accgattaaa ataacttctt
     2521 gatcattatt agtacatact acgacattgt tgttcaatgt tttagtaaca atatattctc
     2581 ccattatcat cacctat
//
LOCUS       DAHHYJ010000208         2583 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000208 DAHHYJ010000000
VERSION     DAHHYJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.212"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..832
                     /locus_tag="NBK22_002542"
     CDS             <1..832
                     /locus_tag="NBK22_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415696.1"
                     /translation="AQILLGLSYSMMAGVDTAIIKRNITNEKYVQNKSNSYMFLSLLI
                     SGIIGSYLYGINIKWPIIMTSIFSILTIIIIRCTLVENRELNLIGETKGKIKKFLPEE
                     KFWILHYSFLRALILGFFIGFIPINIYNDLKLNNLQFISVLTCYTVMGFVSSRYLTKY
                     LNYKFVSEICLVIFLIIYTYQSFIAVTISMIFLGISSGLTRPQTINKLSSSSNLRVML
                     NYAETLYFIFNIAFLLMGGYLYTIGTIQYLILFISLLIFIYLIIIFYFTRREQHENKN
                     "
     gene            816..1331
                     /locus_tag="NBK22_002543"
     CDS             816..1331
                     /locus_tag="NBK22_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCD2415697.1"
                     /translation="MKIKTEFKGNNIPYEYAAGADVSDSINGNPIKSFPFEVIELPEG
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVSGNSISIKADLSRTKGDYVQGKNSFTS
                     GLLAEDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQHKID
                     QTSINLIGRKI"
     gene            1643..2077
                     /locus_tag="NBK22_002544"
     CDS             1643..2077
                     /locus_tag="NBK22_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD2415698.1"
                     /translation="MKLKSFITVTLALGMIATTGATVAGNEVSAAEKDKLPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDGSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
     gene            complement(2334..2519)
                     /locus_tag="NBK22_002545"
     CDS             complement(2334..2519)
                     /locus_tag="NBK22_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCD2415699.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNVVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
BASE COUNT          980 a          310 c          399 g          894 t
ORIGIN      
        1 ggcacaaata ttattagggt taagttactc aatgatggcg ggtgttgata ccgcaataat
       61 taaaagaaat ataacaaatg agaaatacgt acaaaataag tcaaatagct atatgttcct
      121 atcattatta atttcaggga ttataggtag ttatctttat ggaataaata ttaaatggcc
      181 tataataatg actagtatat tttcaattct aacaattata attattcgat gcacattagt
      241 tgaaaatagg gaattaaatt taataggaga aacaaaggga aagataaaga aatttctacc
      301 agaagagaag ttttggatat tgcattattc ttttttaaga gcgttaatat taggattttt
      361 tataggattt attccaatta atatatataa tgatttaaaa ctgaataatt tacaatttat
      421 ttcagtatta acttgttaca cagttatggg ttttgtatct tcacgttatt taactaaata
      481 cttgaattat aagtttgtgt cagaaatttg tttagtaata tttttaataa tatatacata
      541 tcaaagtttc atagcagtta ctatttctat gatattttta ggtatttctt cagggttaac
      601 tcgtccacaa actataaata aactttctag cagtagtaac ttaagagtga tgcttaatta
      661 tgcagaaacg ttatatttta tttttaatat cgcattttta cttatgggtg gttacttata
      721 tacaatagga actattcaat acttaatatt atttatttcg ttattaattt ttatatattt
      781 aataataata ttttatttta caaggagaga gcaacatgaa aataaaaact gaatttaaag
      841 ggaacaatat accatatgaa tacgcagcag gtgcagatgt gagtgattct attaacggga
      901 atccaattaa gtcatttcca tttgaagtaa ttgaattacc ggaagggact aaatatcttg
      961 cttggtcttt aattgactat gatgcaattc ctgtgtgtgg ctttgcatgg attcattgga
     1021 gtgttgctaa tgtaagtgtt agtggcaatt caatttctat aaaagcagat ttatcaagaa
     1081 caaagggtga ctatgtacaa ggtaaaaata gctttactag tgggttgtta gctgaagatt
     1141 tttcagaaat agaaaatcac tatgtaggac ctacaccacc tgatcaagat catcaatatg
     1201 aattaacagt ttatgcgtta gatcattctt taaatttgaa gaatgggttc tacttgaatg
     1261 aatttttaaa agaagtaaat caacataaaa ttgatcaaac aagtattaac cttataggaa
     1321 gaaaaattta atactaaata tctcatcaat ataaaaatgt tcaattaaaa gtacaaagaa
     1381 acaaaggttt taatttatat attaggtacg gcgttcgcta taatgcaaag aagtaattaa
     1441 atttaagaaa tgtaaactta gttattgtaa tgtgaattta tttgaaaaaa atagaaagta
     1501 ttaacaatta tagcttttac attaattaaa atttattttt aaaaacaagt aaacaattta
     1561 catacttata atttttgaaa cttttcaatt tgtgttatat tgattttgta agatacttta
     1621 actcacaaag gagagagatt atatgaaatt aaaatcattt ataactgtaa ctttggcact
     1681 gggcatgatc gcaacgactg gcgctactgt ggcaggtaat gaggtatctg cagcagaaaa
     1741 ggacaaacta ccggcaactc aaaaagctaa agaaatgcaa aatgttccat atacaattgc
     1801 agtagatggc attatggctt tcaatcaatc ttacttaaat ttaccaaaag atagccaatt
     1861 atcatattta gatttaggaa ataaagttaa agctttatta tatgatgaac gcggtgtaac
     1921 acctgagaag attcgaaatg caaaatctgc cgtttacacg attacttgga aagatggtag
     1981 taaaaaagaa gtggatctta agaaagatag ctacacagca aacttgtttg attcaaattc
     2041 aattaaacaa attgatatta atgtaaaaac taaataaaca cttaaaatta tcaaattcaa
     2101 catgaagcat ttaattttac agtgatgatt ataatataat tgccttgata caaagattac
     2161 tcgtaaatga catctttgta ttaaggcttt ttctaaattt aaaagtgatg ggttagaggt
     2221 cattgagctt taaaatattc aaaatacaaa acattaatgg ccaaaaataa aagccgcctt
     2281 tatctgggca gcttcaataa taagaaagac atatttcatt ttatactaaa tagttattgt
     2341 gatgaatctt tcggcggttt aattactgca gcaaaaattg ctgtgaaaat cgtgaacaat
     2401 actgccatga taattggatt cactacattt aagctgtctc cacctactag gctattaagt
     2461 acaaagttaa ccatttgcat taataataat gcccaaaaga atgttacgag gtgtttcatg
     2521 tcattctacc tccactttaa ttatatatat tttattttaa gtgaaagtta gaaatttgta
     2581 tag
//
LOCUS       DAHHYJ010000209         2576 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000209 DAHHYJ010000000
VERSION     DAHHYJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.213"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            293..1096
                     /locus_tag="NBK22_002546"
     CDS             293..1096
                     /locus_tag="NBK22_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCD2415700.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            1315..>2576
                     /locus_tag="NBK22_002547"
     CDS             1315..>2576
                     /locus_tag="NBK22_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD2415701.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQN
                     IDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYN
                     RDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHTPSISDDKDNVMRENHIVDDNPDNDINTPSLSKIDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNK
                     GRATNNNYSDILKNFWMMYWPKLVILMG"
BASE COUNT          969 a          346 c          478 g          783 t
ORIGIN      
        1 tgtataatcg aatttgttac attaaaaata attgtttcgt tgaagaaaaa taaattgtat
       61 attttaaaag aaaaaggtat actatgatgt atcaaatgaa taacctatgg cattttgtca
      121 gaggggagta acttaagaat catgaccgta taaatgattc gacactttat cgtcattacg
      181 aagatatctt ccggtaaagt gggcaattta aattgcttag tgagaccttt gctatttatt
      241 tagcataggt ctttttgttt gtacttaact tatttattta aaggagttgt acatgttaat
      301 ggatccaagt ttgatcttac cttatttatg ggtacttgtc gttttagtat ttttagaagg
      361 cttattagca gcagataacg cgattgttat ggctgtaatg gttaagcact taccacccga
      421 acaacgtaaa aaagctttgt tttacggttt gttaggtgca tttgtattta gatttttagc
      481 attattctta attagtatta tcgcgaactt ttggtttatt caagctgcag gagcggttta
      541 cttaatttat atgtcaatca aaaatctgtg gcagttcttt aaacacccag aaattgaaag
      601 tcctgaagct ggagatgatc atcattatga tgaatctggt gaagagatta aagcaagtaa
      661 caaatcattc tggggaactg tgttgaaaat agaatttgca gatatcgcat ttgccattga
      721 ttctatgctt gctgctttag ctattgctgt aacacttcct aaagttggta ttcactttgg
      781 tggtatggac ttaggtcagt tcgtagtcat gttcctaggt ggaatgattg gtgttattct
      841 aatgcgttat gcagcaacat ggtttgtaga gctattaaac aaatatccag gacttgaagg
      901 tgcagccttc gcgatcgttg gttgggtagg tgttaaatta gttgtcatgg tattagcgca
      961 cccagacatc gctgtattgc ctgagcactt cccacatggc gtattatggc aatctatttt
     1021 ctggacagta ctaattggat tagtaattat cggttggtta ggttcagttg ttaaaaataa
     1081 aaaatcgcat aaataattga tgtgaagcgg acaatcttaa tttagtttaa ggttgtcctt
     1141 tttcatttaa ttgagtgatt tatgaaaaat ggattttgaa gaatgtgaat caaaagatgc
     1201 gatatagtat taagaaaatg tgccttttat atttagcatt ttttcaatag aaattatata
     1261 gattttaaag caaattaggt gttaatgtgt cataatgata agtgatttta ttgaatggag
     1321 tggacattag tggatattgg taaaaaacat gtaattccta aaagtcagta ccgacgtaag
     1381 cgtcgtgaat tcttccacaa cgaagacaga gaagaaaatt taaatcaaca tcaagataaa
     1441 caaaatatag ataatacaac atcaaaaaaa gcagataagc aaatacataa agattcaatt
     1501 gataagcacg aacgttttaa aaatagttta tcatcgcatt tagaacagag aaaccgtgat
     1561 gttaatgaga ataaagctga agaaagtaaa agtaatcagg atagtaagtc agcatataac
     1621 agagatcatt atttaacaga cgatgtatct aaaaaacaaa attcattaga ttcagtggac
     1681 caagatacag agaaatcaaa atattatgag caaaattctg aagcgacttt atcaactaaa
     1741 tcaaccgata aagtagaatc aactgaaatg agaaagctaa gttcagataa aaacaaagtt
     1801 ggtcatgaag agcaacatgt actttctaaa ccttcagaac atgataaaga gactagaatt
     1861 gattctgagt cttcaagaac tgattcagac agctcgatgc agacagagaa aataaaaaaa
     1921 gacagttcag atggaaataa aagtagtaat ctgaaatctg aagtaatatc agacaaatca
     1981 aatacagtac caaaattgtc ggaatctgat gatgaagtaa ataatcagaa gccattaact
     2041 ttaccggaag aacagaaatt gaaaagacag caaagtcaaa atgagcaaac aaaaacctat
     2101 acatatggtg atagcgaaca aaatgacaag tctaatcatg aaaatgattt aagtcatcat
     2161 acaccatcga taagtgatga taaagataac gttatgagag aaaatcatat tgttgacgat
     2221 aatcctgata atgatatcaa tacaccatca ttatcaaaaa tagatgacga tcgaaaactt
     2281 gatgaaaaaa ttcatgttga agataaacat aaacaaaatg cagactcgtc tgaaacggtg
     2341 ggatatcaaa gtcagtcaac tgcatctcat cgtagcactg aaaaaagaaa tatttctatt
     2401 aatgaccatg ataaattaaa cggtcaaaaa acaaatacaa agacatcggc aaataataat
     2461 caaaaaaagg ctacatcaaa attgaacaaa gggcgcgcta caaataataa ttatagtgac
     2521 attttgaaaa atttttggat gatgtattgg cctaaattag ttattctaat gggtat
//
LOCUS       DAHHYJ010000210         2427 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000210 DAHHYJ010000000
VERSION     DAHHYJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.214"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..351
                     /locus_tag="NBK22_002548"
     CDS             <1..351
                     /locus_tag="NBK22_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD2415702.1"
                     /translation="SEDLNYYTDIPKEYNVSVQVFDDLWMDLYDLFKNLRNLFKEEGL
                     EPWTSCEFDFTRDGKLNVSFDYIDWANSEFGQMGREHYYMYKKFGIWPEKEYAINWVK
                     KIKDYVKEQDEAEL"
     gene            362..862
                     /locus_tag="NBK22_002549"
     CDS             362..862
                     /locus_tag="NBK22_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD2415703.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWENIYTIAYVTDQGGEVIF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRETFKEEGLEPWTSSEF
                     DFTSEGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEVKEIEQYIKE
                     QDEAEL"
     gene            873..1361
                     /locus_tag="NBK22_002550"
     CDS             873..1361
                     /locus_tag="NBK22_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin YezG family protein"
                     /protein_id="HCD2415704.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVDDEGGEVVF
                     NYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLEPWTSCEF
                     DFTREGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGVLPETEYEINKVKEVEKYVKE
                     QE"
     gene            1973..2347
                     /locus_tag="NBK22_002551"
     CDS             1973..2347
                     /locus_tag="NBK22_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCD2415705.1"
                     /translation="MKRILVVFLMLAIILAGCSNKGEKYQKDIDKVYKEQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
BASE COUNT          994 a          227 c          453 g          753 t
ORIGIN      
        1 agtgaagatt tgaattatta taccgatata cctaaggagt ataatgtttc tgtgcaagta
       61 tttgatgatt tatggatgga tttatatgat ttgtttaaga atttaagaaa tttatttaaa
      121 gaagaaggac ttgaaccatg gacatcatgt gaatttgact ttacaagaga cggcaaattg
      181 aatgtttcat ttgattatat tgattgggcg aattcagagt ttggacaaat gggaagagaa
      241 cattattaca tgtataaaaa atttggaatt tggcctgaaa aagaatatgc cataaattgg
      301 gtgaaaaaaa taaaagatta tgttaaagag caagatgaag ctgaactata ggggcgataa
      361 tatgactttc gaagaaaaac taagtcaaat gtacaatgaa attgcaaatg aaatcagtgg
      421 aatgatacca gttgaatggg aaaatatata tacaattgcc tatgtaactg atcaaggtgg
      481 agaggtcatt tttaattata ctaaaccagg tagcgatgaa ttgaattatt acacatatat
      541 ccctagagag tataatgtct ctgaaaaagt attttatgat ttgtggacgg atttatatag
      601 attgtttaag aagttaagag aaacttttaa agaagaaggg cttgaaccat ggacatcaag
      661 tgaatttgac tttacaagcg aaggtaaatt aaaagtttca tttgattata ttgattggat
      721 aaatacagag tttgatcaat taggccgtga aaactattat atgtataaaa agtttggtgt
      781 tttaccagaa atggaatacg aaatggaaga agttaaagaa atcgagcaat atattaaaga
      841 gcaagatgaa gctgaactat aggggcgata acatgacttt cgaagaaaag ctaagtcaaa
      901 tgtacaatga aattgcaaat gaaatcagtg gaatgatacc agtagaatgg gaaaaagtat
      961 atacaattgc ctacgtagat gatgaaggtg gagaggttgt ttttaattat actaaaccag
     1021 gaagtgaaga tttgaattat tattcagata ttcctaaaga ttgcaatgtc tcaaaagata
     1081 tttttaagaa ttcatggttt aaagtttatc gaatgtttga tgagttaaga gaaactttta
     1141 aaaaagaaga tttagaaccg tggacatcat gtgaatttga ctttacaaga gagggaaatt
     1201 tgaaagtatc gtttgattat atagattgga ttaaattagg ttttggtcca tcaggaaagg
     1261 aaaactacta tatgtataaa aagtttggtg ttttaccaga aacggaatat gaaattaata
     1321 aagttaaaga agtagagaag tatgttaaag agcaagagta gcagacatgt tatagaaggc
     1381 tgtgcaattt tacatttgat tcaaagaaga aggtaaacga taagattatt tgcaacttaa
     1441 aaagtctatt agcttctcgg tatccataca tcatggataa atgagtttaa ctaattaata
     1501 aatcacgata taaatttaaa attgccatta attgaaaagg ttattgtagt gtattttata
     1561 aagttgatgc acatcgactt attttttaaa acttatatca aaaaaatatg agaaaagtat
     1621 atactattaa aatgtttttt actataatta taaatagtta aatatttggg aattaaataa
     1681 atgtatagga ggcaattttt agagatacat agcaatgtaa aggtatcaaa tagttattgc
     1741 ataatttatt atgatgacat aattttcagt agatagttgt aaattaacac tgaaatagat
     1801 gtttatatta agaagacttt aatatcataa ttaatcaaaa tcttgcgtca aacatttaaa
     1861 cgaattttaa gttgataaca attggcaaaa cattgttcaa acatcacaat gataaagcgt
     1921 attatcagta ttgtagtgtg tggaaaatga cagccatcta aggagaaaaa tgatgaaaag
     1981 aatattggta gtatttttaa tgttagcaat tatattggca ggttgttcta ataaaggtga
     2041 aaagtatcaa aaagatattg ataaagtgta caaagaacag aatcaaatga ataaaattgc
     2101 ctcgaaagta caaaacacta ttaaaacaga cattaaacaa gaagacagta atacacatgt
     2161 ttataaagat ggtaaagtca ttgttattgg tattcaatta tataaagatc gtgaaaaaat
     2221 gtattatttc gcatatgaaa taaaagatgg taaggcagaa atcaatagag aaatagaccc
     2281 aattaagtat atgaaagacc ataaagcaga ttatgaagat gaaaatgtag aagtggaaaa
     2341 agattaacgt atgctttctt taaaactttt aaccagcttc aaaaaataaa tgagctttag
     2401 cgacaataat ttttaaaata aacacga
//
LOCUS       DAHHYJ010000211         2411 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000211 DAHHYJ010000000
VERSION     DAHHYJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.215"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            80..256
                     /locus_tag="NBK22_002552"
     CDS             80..256
                     /locus_tag="NBK22_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCD2415706.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            271..873
                     /locus_tag="NBK22_002553"
     CDS             271..873
                     /locus_tag="NBK22_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD2415707.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWIAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            1106..1251
                     /locus_tag="NBK22_002554"
                     /pseudo
     CDS             1106..1251
                     /locus_tag="NBK22_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226109.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            1790..1891
                     /locus_tag="NBK22_002555"
     CDS             1790..1891
                     /locus_tag="NBK22_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415708.1"
                     /translation="MKFVANKLTLTIFRKFCINNEKEIWIKYEEVFL"
     gene            1901..2173
                     /locus_tag="NBK22_002556"
     CDS             1901..2173
                     /locus_tag="NBK22_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCD2415709.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNCWVNRAYYNIY"
     gene            2217..>2411
                     /locus_tag="NBK22_002557"
     CDS             2217..>2411
                     /locus_tag="NBK22_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCD2415710.1"
                     /translation="MKWFKLILDVTTFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKRINQFAKNEN"
BASE COUNT          848 a          290 c          403 g          870 t
ORIGIN      
        1 atttttgatt gttaatagtt tatttgttaa gtgaatttta gtacaatata atttagttat
       61 gatttaagga ggacttaaaa tgactgttgc agaagtgggt aacattgttg agtttatgga
      121 tggattaaga ggtcgtgttg aaaaaatcaa cgataactct gttattgttg acttaacaat
      181 tatggaaaat tttaatgacc ttgatttacc ggaaaaaact gttatcaatc ataaacgata
      241 taagattgtt gaataagaag gtaagttata atgaataaaa tctcgaaggc tttaacttgg
      301 tttattataa gtttcattat atttcatctc atattattta ttatgtgggg cgaacaccaa
      361 gaatactggt atttatatac aggtataatg ctaattgctg gtatcagtta tgtattttat
      421 caaagagata ttgaatctaa gcggttgctt acatcaattg gtgttggtat tattacggca
      481 attattttaa ttatgcttca acttttattc tcacttataa cttctaattt aagttatagt
      541 tcattaatta aagaattagc aagaacaggt gtcaattgga agtggcaaat gttagtaact
      601 ttactttttg tcattccatg tcacgagtta tatatgagaa ctgttttaca aaaagaatta
      661 acacattttt caataccgaa atggatagct atattaataa ctgcaatttg ttctagttca
      721 ttatttattt atttagataa ttggtggata gtaacattta tatttgttgc acaagtgata
      781 ttatcattaa gttatgagta tactcgacgc atagcaacaa catccgttgc gcaaattgtt
      841 gctattatat tgttattaat ttttaatgct taaataagct tttcttagac gtctgtgttc
      901 taagaaaagc tttttgcttt agaaaagcaa tacgaagatt caaggcatgc tttattatag
      961 tgtatacatt tttaacggca agcagttatt attgaggtta tattttagtc agtttttatt
     1021 actaaatatt ttataaaaat agtataatat tttgtgggca agttgaattg atggtggcta
     1081 tctgagtaaa gggggggtgg tgcctatggc attacttaac acttttgaaa ggaaaagcct
     1141 attgtgatat ctattgcaaa cgcattacat ttaatgttaa gtttcggtat gtttatcgtc
     1201 actttcattg gtatagtagt agcaataata aatttaagca ataaaaaata accatcattt
     1261 caaaactttg acatagaatg atggttaaag aaaatctcat ttagtcaccg tctttttaac
     1321 gggctcatta ggcgacatgt tcctgcatgt cgtcttttta catttttata gtaacatatc
     1381 ttttatttaa ttgtctattt ttattagatg tgtaacaaaa ttttgaagta ttttccgtat
     1441 attgcctgct aaacatgtca gatttgaaag gaaaatcata ctgtggtaga aatcaattat
     1501 gattatatgg tagtacttat aaataagtga tttctattga atatgtaatg aagttcatat
     1561 attaatataa ctttcattga tatagaagtc gcaatgatta atttaaacaa taaaaaacaa
     1621 ccatcatttc aaaactttga caacgaatga tggttaaaga aaatctcatt tagtcaccgt
     1681 ctttttaacg ggttcaatag gggacatgtt cctgcatgtc gtctatttat gcactgcgca
     1741 atttaacaat aatacaatta tactttgatg tccatttaac ataaagtggt tgaaatttgt
     1801 agcaaataaa ttaaccttga ctattttcag aaaattctgt ataaataacg aaaaagagat
     1861 atggattaaa tatgaagagg tgtttttata gaagaataaa atttttggca ctatattata
     1921 tttaacttta gcacttggat tatcaacagc agcttatgca tctacagaat acgcagaagg
     1981 aggcacttgg agtcacggtg tcggcagtaa gtatgtttgg tcttattatt atcatggtca
     2041 taaaggacat ggtgcaacag ctattggaaa atatagatca tttagtggtt atacaagagc
     2101 tggtgtaaaa gcaaaagcat cagctactaa acataattgc tgggtcaata gagcgtatta
     2161 taacatttat taaatactaa aaaatcatag aaaataaaca acgcaagagg tacaacatga
     2221 aatggttcaa attaatactt gatgttacaa ccttcattct gattgctata ttgttatttg
     2281 tttatacata taaagaaaac gaagaaatat tgcctgatac taaataccct atagcggtaa
     2341 ctgactggaa taaaaaatat agtaaaaatg agatttataa acgtataaat caattcgcta
     2401 aaaatgagaa c
//
LOCUS       DAHHYJ010000212         2378 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000212 DAHHYJ010000000
VERSION     DAHHYJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.216"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(76..1269)
                     /locus_tag="NBK22_002558"
     CDS             complement(76..1269)
                     /locus_tag="NBK22_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2415711.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKQSKE
                     IPFIIIIQFACILINTGLWRVVLPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLLGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISLIIPLLSLSNIFISGGAIISMLSLAGLKF
                     VKKSM"
     gene            complement(1653..>2378)
                     /locus_tag="NBK22_002559"
     CDS             complement(1653..>2378)
                     /locus_tag="NBK22_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E domain-containing protein"
                     /protein_id="HCD2415712.1"
                     /translation="VNELTEFGGEKIPQGHKDIFDPNLPTDQTEKVPGKPGIKNPDTG
                     KVIEEPVDDVIKHGPKTGTPETKTVEIPFETKREFNPKLQPGEERVKQEGQPGSKTIT
                     TPITVNPLTGEKVGEGQPTEEITKQPVDKIVEFGGEKPKDPKGPENPEKPSRPTHPSG
                     PVNPNNPGLSKDRAKPNGPVHSMDKNDKVKKSKIAKESVANQEKKRAELPKTGLESTQ
                     KGLIFSSIIGIAGLMLLARRRKN"
BASE COUNT          766 a          432 c          321 g          859 t
ORIGIN      
        1 aaattataag ccaaactaat tttatatttc atttcaattc ccaccttaat agcattgatt
       61 aaaattctat tatgtttaca tagacttctt aacaaacttt aacccagcta agctaagcat
      121 tgaaataatt gcacctcctg atataaatat attacttaag cttagtaaag gtataattaa
      181 acttataagt cctaaagaca atgtatccgc tgcgtaattc gatgtagatg agatactaaa
      241 tactttcccc atcaaatgat ttggcgtttt tatttgaatg gcaactgatc ttgttagtcc
      301 ctctatagat tgtccaagtc ccaataatgt tgcacctata tataatatcg ccacacttgg
      361 aaacacatta ataatcgata agccaattcc ccaaactaaa acaccaatac taaatttaaa
      421 gattaatcgc ttttctgaca gcaaacccat aatcaatgac attaataaag atgctatacc
      481 taaacatgat gtagctagtc catatacacc aacgccctct tttaatatat tggaaataaa
      541 caatggtaat acaacacgcc aaaggccagt attaatcaat atgcaggcaa attggatgat
      601 tataataaat ggaatttctt tagattgttt caagaattcc caagtttcag aaaaatcttc
      661 ttttgagtgt ctatcaatca tgttgttatt tgtatatttt aaaagtgcat taaaaataaa
      721 tcctaaaaat agcaatatac tacaaataaa aaagacgcca acattaccaa ctagtattac
      781 aatgacacca attaaagcag gtaaaataat atttgagcct ctttgcaaac tatcgattaa
      841 cgcattactt gttgctaaat gctcctcatc aataatttca ggaagaattg ccctaaacgc
      901 aggatccgta tagcagttaa taatggtgat agctgtagat atggttagaa gcgtcagata
      961 atttaaattt gatgttattg caagtaaagg aataattatt ataatcaaac ttagtataag
     1021 atcagataga taaagtattt tctttttatt atgtttatca gaatatgcgc caccgaaaat
     1081 accaaatata ataaatggaa gtgtttgact cataaccatc attgataatt ttaaagatga
     1141 ttggtttgtc aattcaacag taaaccaaat tatttgtaac gaaaacagca caaaacaact
     1201 ccgacgtaag aaattaccaa tcaataaata tgtaaagttt ctatttttca aaacttctaa
     1261 gtacaacata tttatcacct ctcataaaaa taattgaatg catccaccag cctttttaga
     1321 ccttcttcta aactctcttt atccaaagcg caattaattc taatataatt tagtcagtta
     1381 aatatcaatt atttcgaaat atacatacta cttgaaacac catacataac ccccaaaatg
     1441 actactcaga ggttatattc tactaattat gattatatta aatatgaaaa tattatcaaa
     1501 aaaatcaaat ttataacaaa aatacacccc ttaaagttag gtctttcaat ccaacttttg
     1561 gggtgtatat cattctcatc atattctagg ttgtttttaa caaactaaat atagtgaatg
     1621 caaatcaact attatttaaa ttatgaatta ttttaattct ttcttctacg agccaataac
     1681 attaatccag caattccaat tatactacta aagatcaaac ctttttgcgt gctttctaaa
     1741 cctgtttttg gtaattctgc tcgttttttc tcttgattag ctactgattc tttagcaatt
     1801 ttagattttt taactttatc atttttatcc attgaatgaa ctgggccatt tggttttgct
     1861 ctgtctttcg ataatcctgg attgttagga tttactgggc cacttggatg agttggtctg
     1921 ctcggcttct ctgggttttc aggtcctttt ggatcttttg gtttctctcc accgaactct
     1981 acaatcttat ctactggttg ttttgtgatc tcttctgttg gttgaccctc gccaactttt
     2041 tcacctgtta atgggttcac tgtgattggt gttgtgattg tcttacttcc tggttgtcct
     2101 tcttgtttca ctcgctcttc accaggttgt aattttggat taaactcacg ttttgtttca
     2161 aacggtatct ctactgtttt tgtttctggt gtacccgttt ttggtccgtg tttaatcaca
     2221 tcatccactg gctcttcgat cacttttcct gtgtctggat tcttgattcc tggtttacct
     2281 ggtacttttt ccgtttgatc tgttggtaag tttggatcaa agatatcttt atgaccttgc
     2341 ggtattttct cgccaccgaa ttctgttaat tcattaac
//
LOCUS       DAHHYJ010000213         2369 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000213 DAHHYJ010000000
VERSION     DAHHYJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.217"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            31..306
                     /gene="rpsP"
                     /locus_tag="NBK22_002560"
     CDS             31..306
                     /gene="rpsP"
                     /locus_tag="NBK22_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCD2415713.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            494..997
                     /gene="rimM"
                     /locus_tag="NBK22_002561"
     CDS             494..997
                     /gene="rimM"
                     /locus_tag="NBK22_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCD2415714.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQEAPIGRVINIFETGANDVWVIKGSKEYLIPYIAEVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            997..1734
                     /gene="trmD"
                     /locus_tag="NBK22_002562"
     CDS             997..1734
                     /gene="trmD"
                     /locus_tag="NBK22_002562"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCD2415715.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            1837..2187
                     /gene="rplS"
                     /locus_tag="NBK22_002563"
     CDS             1837..2187
                     /gene="rplS"
                     /locus_tag="NBK22_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCD2415716.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
BASE COUNT          830 a          352 c          465 g          722 t
ORIGIN      
        1 ttaaaacatg acttaaagga gattttataa atggcagtta aaattcgttt aacacgttta
       61 ggttcaaaaa gaaatccatt ctatcgtatc gtagtagcag atgctcgttc tcctcgtgac
      121 ggacgtatca tcgaacaaat cggtacttat aacccaacga gcgctaatgc tccagaaatt
      181 aaagttgacg aagcgttagc tttaaaatgg ttaaatgatg gtgcgaagcc aactgataca
      241 gttcacaata tcttatcaaa agaaggtatt atgaaaaaat ttgacgaaca aaagaaagct
      301 aagtaattta gcgtaaaatt gttctaacaa taagaataac tcgtttacac tgacagttat
      361 tactcaatga tacgttggga atatcacatg ttagtaatat agaacgtatt gggtaccata
      421 atggtgccct ttttctttga attattttca attaaaatag aagtgttcaa agcatagagt
      481 tggaggtaat agaatgagag ttgaagttgg tcaaattgtt aacacacatg gtattaaagg
      541 tgaaattaaa gtaaaatcca attcagactt tacagacgtt cgttttcaac ccggtcaagt
      601 gctgacagtt gtgcataaca ataacgacct tgaatatact gttaagtcac atagagtgca
      661 taaagggctt catatgctca catttgaagg tattaataat attaatgata ttgagcattt
      721 aaaagggagt tctatttatc aagagcgtga tcatgaagat atcgtacttg aggaaaatga
      781 attttattat tcagatatta taggatgtac agtttttgat gatcaagaag caccaatcgg
      841 tcgtgttatc aacatctttg aaacaggtgc aaatgatgta tgggtaataa aaggttcgaa
      901 agaatattta attccataca ttgctgaagt ggtaaaagaa gtggatgttg aaaataaaaa
      961 aatcatcatt acaccgatgg aaggattgtt ggattaatga aaattgatta tttaacttta
     1021 tttcctgaaa tgtttgatgg tgttttaaat cattcaatta tgaaacgtgc ccaagaaaac
     1081 aataaattac aaatcaatac ggttaatttt agagattatg caattaacaa gcacaaccaa
     1141 gtagatgatt atccgtatgg tggcggacaa ggtatggtgt taaagcctga acctgttttt
     1201 aatgcaatgg aagacttaga tgtcacagaa caaacacgcg ttattttaat gtgtccacaa
     1261 ggcgagccat tttcacatca gaaagctgtt gaattaagca aggctgacca catcgttttc
     1321 atatgcggac attatgaagg ttacgatgaa cgtatccgaa cgcatcttgt cacagatgaa
     1381 atatcaatgg gtgactatgt tttaacaggt ggagaattgc cagcgatgac catgactgat
     1441 gctattgtta gactgattcc aggtgtttta ggtaatgaac agtcacatca agacgattca
     1501 ttttcagatg ggttattaga gtttccgcaa tatacacgtc cgcgtgaatt taaaggtcta
     1561 acagttccgg atgttttatt gtctggaaat catgccaata ttgatgcatg gagacatgag
     1621 caaaagttga tccgcacata taataaaaga cctgacttaa ttgaaaaata tccattaact
     1681 aatgcagata agcaaatatt agaaagatat aaaataggat tgaaaaaagg ttagtattgt
     1741 gctaacataa tctgagtgtg gaaacacaaa aatcactatg atccgctgct atatatttgt
     1801 cgaggcaaga acataggttg aagaggagaa tttataatga caaatcacaa attaatcgaa
     1861 gcagtaacta aatcacaatt acgtacagac ttacctagtt tccgtcctgg tgatacttta
     1921 cgtgtacacg tacgtatcat tgagggtact cgtgagcgta tccaagtatt cgaaggcgtt
     1981 gtaattaaac gtcgtggcgg tggcgtttct gaaacgttta cagttcgtaa aatttcatca
     2041 ggtgttggcg tggaacgtac attcccatta cacacaccaa aaattgaaaa aatcgaagtt
     2101 aaacgtcgtg gtaaagtacg tcgtgctaaa ttatattact tacgtagttt acgtggtaaa
     2161 gctgctagaa tccaagaaat tcgttaatca gcatttaaac aaagctatgt atgagtcaaa
     2221 ttcgacttaa acaataaagc catctaggtc acttttatgg agtgatatag atggcttttt
     2281 ttgctgttta gttaatgtaa agttgataat gtaattgttt attccttctc tattcataac
     2341 gtgtttgctc aaaatggtta tataaacaa
//
LOCUS       DAHHYJ010000214         2360 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000214 DAHHYJ010000000
VERSION     DAHHYJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.218"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            183..1742
                     /gene="rny"
                     /locus_tag="NBK22_002564"
     CDS             183..1742
                     /gene="rny"
                     /locus_tag="NBK22_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCD2415717.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
     gene            complement(2039..2254)
                     /locus_tag="NBK22_002565"
     CDS             complement(2039..2254)
                     /locus_tag="NBK22_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415718.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
BASE COUNT          885 a          336 c          439 g          700 t
ORIGIN      
        1 tcgagcttca aaactttaga aaatagtaga aatagcattc aatatagtgc aaaagtgcaa
       61 attgataact tgacacttat ctcctataaa ccgtacaatt aatttgtatg atttatatat
      121 aatttcataa agtcatattg aatttcatat aaagagcaaa ccctagaaaa ggaggtgttt
      181 gtgtgaattt attaagcctc ctactcattt tgctggggat cattctagga gttgttggag
      241 ggtatgttgt tgcccgaaat ttgttgcttc aaaagcaatc acaagctaga caaactgccg
      301 aagatattgt aaatcaagca cataaagaag ctgacaatat caaaaaagag aaattacttg
      361 aggcaaaaga agaaaaccaa atcctaagag aacaaactga agcagaacta cgagaaagac
      421 gtagcgaact tcaaagacaa gaaacccgac ttcttcaaaa agaagaaaac ttagagcgca
      481 aatctgatct attagataaa aaagatgaga ttttagagca aaaagaatca aaaattgaag
      541 aaaaacaaca acaagtagat gcaaaagaga gtagtgttca aacgttaata atgaagcatg
      601 aacaagaatt agaacgcatc tccggtctca ctcaagaaga agctattaat gagcaacttc
      661 aaagagtaga ggaagaactg tcacaagata ttgcagtact tgttaaagaa aaagaaaaag
      721 aagctaaaga aaaagttgat aaaacagcaa aagaattatt agctacagca gtacaaagat
      781 tagcagcaga tcacacaagt gaatcaacgg tatcagtagt taacttacct aatgatgaga
      841 tgaaaggtcg aatcattgga cgagaaggac gaaacatccg cacacttgaa actttaactg
      901 gcattgattt aattattgat gacacaccag aagcggttat attatctggt tttgatccaa
      961 taagaagaga aattgctaga acagcacttg ttaacttagt atctgatgga cgtattcatc
     1021 caggtagaat tgaagatatg gtcgaaaaag ctagaaaaga agtagacgat attattagag
     1081 aagcaggtga acaagctaca tttgaagtga acgcacataa tatgcatcct gacttagtaa
     1141 aaattgtagg gcgtttaaac tatcgtacga gttacggtca aaatgtactt aaacattcaa
     1201 ttgaagttgc gcatcttgct agtatgttag ctgctgagct aggcgaagat gagacattag
     1261 cgaaacgagc tggactttta catgatgttg gtaaagcaat tgatcatgaa gtagaaggta
     1321 gtcatgttga aatcggtgta gaattagcga aaaaatatgg tgaaaatgaa acagttatta
     1381 atgcaatcca ttctcatcat ggtgatgttg aacctacatc tattatatct atccttgttg
     1441 ctgctgcaga tgcattgtct gcggctcgtc caggtgcaag aaaagaaaca ttagagaatt
     1501 atattcgtcg attagaacgt ttagaaacgt tatcagaaag ttatgatggt gtagaaaaag
     1561 catttgcgat tcaggcaggt agagaaatcc gagtgattgt atctcctgaa gaaattgatg
     1621 atttaaaatc ttatcgattg gctagagata ttaaaaatca gattgaagat gaattacaat
     1681 atcctggtca tatcaaggtg acagttgttc gagagactag agcagtagaa tatgcgaaat
     1741 aatttttgtc tccctcacaa attagtgagg gagctttttt aagttgtagt ctcaatctaa
     1801 ttagacagca ctttatcggt aataactata gtaaacagtg gttatttgaa agtaagacgg
     1861 accttatatt aaataagaag ttattgcttt taataaaaat gttttaggct tcgtaattac
     1921 tatatttata ttatgtaaac ctataaagat gattgctttt ctatccaata aaaaagaaga
     1981 ggagatgtaa cacatcttct cttctgcaat attaattagg atttatttct aagttgagtt
     2041 attttaattg taaatctgtt ttctttaatt cttttataac ttctgcagta tcataacaat
     2101 ttgttgcaat tgttgaatat ctctctgcta aacgatatgc attaatgtaa agctttaaac
     2161 tttctttagc tatatcctct gcatcttcga attttgatgg gttagacata accactaatt
     2221 ctgcaaattt ttctggatca atattaatag acatgtattt atttacaact cctatttatt
     2281 ttgatgtctt aatactaaca tattgaagtt ttcagacaaa gtaatgtctc tctataattg
     2341 aagaaaaata attcaaagaa
//
LOCUS       DAHHYJ010000215         2312 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000215 DAHHYJ010000000
VERSION     DAHHYJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.221"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            8..124
                     /locus_tag="NBK22_002566"
     CDS             8..124
                     /locus_tag="NBK22_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415719.1"
                     /translation="MSIENMRTYQQRVNNLWIKGVDNVIYCGYQVGKQKIND"
     gene            complement(269..2197)
                     /gene="abc-f"
                     /locus_tag="NBK22_002567"
     CDS             complement(269..2197)
                     /gene="abc-f"
                     /locus_tag="NBK22_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCD2415720.1"
                     /translation="MILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKST
                     LMKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIK
                     EETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLYGLNFSEEDF
                     NKPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVI
                     ISHDRYFLDKIVTQIYDVALGDVKRYVGNYEEFIQQRDLYYQKRMQEYESQQAEIKRL
                     ETFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVK
                     NLEIGYQTAITKPMSIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANL
                     QIGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGG
                     EKARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQ
                     LANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHN
                     QKEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDS
                     EQKLEHAMSKWEELQQKL"
BASE COUNT          686 a          377 c          345 g          904 t
ORIGIN      
        1 aaaaacaatg tcaatagaga acatgcgtac ttatcaacaa agagtgaata acttgtggat
       61 aaaaggtgtt gataacgtca tttattgtgg atatcaggtg ggaaaacaga aaataaatga
      121 ttgattagtg ttaatttact tgtggataac taggataagt ctgtggatag ctataatatc
      181 agcgttttta ttgtgaataa aaaaaattaa aatttgtaaa aaaataactc taactatagc
      241 gattttacag ttagagttat gtatattttt atagtttttg ttgtaattct tcccatttag
      301 acatagcgtg ttctaatttt tgttcgctat cttgtttttg gatagctaat tcgtttgctt
      361 tttgtggatt gttatacact tctggttggg ttaattgctc gtctatttgt aagatagtag
      421 tctctaaagt ttctatttca ttttcacact cggatatttg tcgttcaagt ttgcgttgtt
      481 cacgtctttg ttctttttgg ttatgatacg acgattgttc catagacttt ttatgtgctt
      541 gtacgttaac tgactgttct tcttggtgcg ctttcaacgc ttccgcttcc tcaacttttt
      601 caatgtaata ttgataatct cctaaataca tctttccgcc atcaattgtt aaatcaaata
      661 ccttgtttgc taattggtta ataaagtaac gatcatggga aacaaatagt attgttcctt
      721 caaaatgttg gagtgcttgc tctaacattt cttttgaatc tatatcgagg tggttggtag
      781 gttcatctaa aataagtaca ttgtcacgtt gcaacataag tagtgctagt tgtaaacgtg
      841 ctttttcacc accagataaa tcattaatta tctttttaac atcgtcttgt acaaataaga
      901 aacgtccaag aactgctcga atatcttttt cattcattaa cggatattga tcccacacat
      961 aatctaaaat cgttttacta gatttaaatt ctgcttgctt ttgatcataa taaccaattt
     1021 gtaaatttgc gccgaaagta atatcgccat taagcgcttt ttgttgatta gcaatagttt
     1081 taattaaggt cgattttcca ataccatttg gcccaatgat tgctatatga tcgcctttag
     1141 agacctctat actcataggt ttggtaattg cagtttgata accgatttct aaatttttta
     1201 catgcatgac gtcattacct gtattccggt caaagccaaa ttgaatattt gcacttttgg
     1261 catctaacat tggtttatca atgcgttcca ttttttctaa aatcttacgt ctactttttg
     1321 ccattccact tgttgaagca cgggtaatat ttttctcaac aaaagtttct aatcgtttta
     1381 tttctgcttg ttgactttca tattcttgca ttcgtttttg ataatataaa tcccgttgct
     1441 gtataaattc ctcgtaatta ccaacatagc gtttgacatc acctaaagcc acatcataaa
     1501 tttgagtaac tattttatct aaaaagtaac gatcatggct gatgatgacg attgcacctt
     1561 taaaataacg tagataatct tcaagccact ttgtcgtttc taaatctaag tggttagtag
     1621 gttcatctaa aagtaataaa tcaggttcgt ttaatagcat ttgagctaaa gataaacgtg
     1681 ttttttggcc accgctaaaa tcattgatag gtttattgaa atcttcttca ctaaaattta
     1741 acccgtaaag tactgtttta attttacttt catattgata tccttctaat tgttcaaatt
     1801 gattcgataa agattcataa cgggacatat gtgttttata tgtatcacta tcgtaatcat
     1861 ttgcatgttt tgataaccaa tctgtttctt ctttgattaa gctttccatt cgtttaatat
     1921 gttcaaatgg ttttgacatt tcttcaaaaa ctgttgcatt agaatttaaa gtcatttgtt
     1981 gagttaaata acctagtttt aagtttttaa tttttgacac atttccagaa tcatagtttt
     2041 ctacgccagc tataattttc atcaatgttg atttaccggc gccatttctt cctacgatac
     2101 caattcgttc accagttttt acttcaaaat caacatcagt aaatatatct tcaccatcga
     2161 acgattttga tatatgattg agttgtaaaa gtatcattcg atcttcacct ttcgtctttc
     2221 gcaaatttta ttaacttatg aaaaaagtcg taattattac attaataacc attttacagt
     2281 ataaaacgcc gtcttgaaac taagatattt at
//
LOCUS       DAHHYJ010000216         2283 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000216 DAHHYJ010000000
VERSION     DAHHYJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.222"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            11..390
                     /gene="rnpB"
                     /locus_tag="NBK22_002568"
     ncRNA           11..390
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NBK22_002568"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            599..1744
                     /locus_tag="NBK22_002569"
     CDS             599..1744
                     /locus_tag="NBK22_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCD2415721.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            1828..2160
                     /locus_tag="NBK22_002570"
     CDS             1828..2160
                     /locus_tag="NBK22_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCD2415722.1"
                     /translation="MGKKMGLGLSISLIVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDDGKTFYKQEIVKHYDVPHGLMI
                     KIHDMHMN"
BASE COUNT          818 a          307 c          475 g          683 t
ORIGIN      
        1 ttaaaagtga tattttgggt aatcgctata ttatatagag gaaagtccat gctcacacag
       61 tctgagatga ttgtagtgtt cgtgcttgat gaaacaataa atcaaggcat taatttgacg
      121 gcaatgaaat atcctaagtc tttcgatatg gatagagtaa tttgaaagtg ccacagtgac
      181 gtagctttta tagaaatata aaaggtggaa cgcggtaaac ccctcgagtg agcaatccaa
      241 atttggtagg agcacttgtt taacggaatt caacgtataa acgagacaca cttcgcgaaa
      301 tgaagtggtg tagacagatg gttatcacct gagtaccagt gtgactagtg cacgtgatga
      361 gtacgatgga acagaacatg gcttatagaa atatcactac tagtttagct ctcctagatg
      421 atggagagct tttttcatga aaagaacact taaaattaac gccttgtctt gatataatga
      481 cactgccttg ttttaaaata gtaagcggat gcgttaatgt atcagcgatt aaatttgttg
      541 gaaatgtata aaaaacacaa gctaagaata aaatacctgt ataaaaggag aatcatatat
      601 gtttcaatta cttgcagttt gtccgatggg attagaagct gttgttgcta gggaaattca
      661 agaattaggc tatgaaacaa atgttgaaaa tggtcgtata ttttttgaag gagacgcaag
      721 tgcaattgta aaggcaaatt tatggttgcg cacagcagac cgaatcaaaa ttgttgttgg
      781 acgttttaac gcaacaacgt ttgacgaatt attcgaacaa accaaagcgc tcccttggga
      841 atctataatt gataaagaag gtaacttccc agttcaaggt agaagcgtta aatcaacact
      901 acatagtgtg ccagattgtc aggcgataac taagaaagct attgttgaac gattacgtcg
      961 cgcatataac gaaaaaggtt ggttaaatga atcaggtgcc aaataccctg ttgaagttgc
     1021 cattttaaaa gataatgtat tattgactat agacacatca ggttctggtt tgaacagacg
     1081 tggatataga ttagcacaag gtgaagcacc aattaaagaa acgttggcag caagtttaat
     1141 ccgtcttgca aactggaaag gtgatacacc tttaattgat ccattttgcg gttcgggtac
     1201 aatagctata gaagcttgtt taattgctca aaatatcgca cctggtttta atcgcgagtt
     1261 cgtatcagag caatggaaca tcatgccagc aaatatttat gatgattacc gtgatgaagc
     1321 ggataagatg gctgattatg ataaagaaat cgaagtatat gcttctgata ttgatccaga
     1381 aatggtagag attgctaagc gtaacgctga agaagttggg ttgtctgata ttattaaatt
     1441 tagtgtaaaa gatgtcaata cattaacaat tgatacagaa gaaccggtgg cgttaattgg
     1501 aaatcctcca tatggtgaac gtattggtga tcgtgaagaa gttgaagaaa tgtaccgtta
     1561 tattggtaaa ctaatgaaac aacatccatt tttatctaca tacattttaa caagtaataa
     1621 agaatttgaa tatttagtag atcgtaaagc aacgaagcga cgtaagttat ttaatggata
     1681 tatagaatgt acttattatc aatactgggg taaaaagaca gaacgtaaaa ctatcgaaaa
     1741 ctaaagtgtt tctaatgcgt gactaaaatt agtattaatt atgttctcat gataataggt
     1801 gtttttgaaa aatggaggag tctataaatg ggtaaaaaaa tgggtctagg tttatctata
     1861 tcattgattg ttattggtat tgccgttgta tgtttaatga ttttttctag tcaaaaaacg
     1921 acttattttg gttatatgaa tagtaataca aatgcagaaa aagttgtaag tgaaaaagat
     1981 ggattagtca aacataatat caaagtagaa ccatctaatg atttcaagcc gaaaaaagga
     2041 gactttgtaa aattagtttc taaagatgat gggaagacat tttataaaca agagattgtt
     2101 aaacattatg acgtcccaca cggtttaatg ataaaaattc acgacatgca tatgaattaa
     2161 taaaaaagca tctataacgt aattttgaag aagtagagtt atcttcttat gcgttttaga
     2221 tgctttttta tcttcaaatt caaaaaatcg gacagatggg agtgggacag aaatgataaa
     2281 gag
//
LOCUS       DAHHYJ010000217         2265 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.223, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000217 DAHHYJ010000000
VERSION     DAHHYJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.223"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..1335
                     /locus_tag="NBK22_002571"
     CDS             <1..1335
                     /locus_tag="NBK22_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCD2415723.1"
                     /translation="KDEYLAENIFWVPKESRWQYINDNSKKPEIGQIIDKAMIAIERE
                     NESLKGVLPKDYARPALDKEKLGDIIDLFTFKVGDSESKKQDVLGRVYEYFIAKFASA
                     EGKNAGEFYTPASIVKLLVEMIEPYQGRIYDPCCGSGGMFVQSERFVERHQGRLDNIA
                     VYGQESNPTTWKLAKMNLAIRGIDNDLGDHHADTFHNDLHKDLKADFILANPPFNASD
                     WGREKLLDDYRWKFGIPPKGNANYAWIEHMISKLAPSGTAGFVLANGSMSTSGKDELE
                     IRKNLIEQDLVECIVTLPGQLFYSTQIPVCLWFVTKNKAKNGKNERRGEVLFIDARNI
                     GSMVSRTLKEFSDDEIKDIANVYHSWRGTNNNQYEDKAGFCKVAKTEEIKNNEYILTP
                     GRYVGLAEVEEDSEPFEQKMERITADLSEQFAKSKELEDQIRKSLEGLGYGV"
     gene            1325..>2265
                     /locus_tag="NBK22_002572"
     CDS             1325..>2265
                     /locus_tag="NBK22_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD2415724.1"
                     /translation="MEFETFNLTDLYTISSGLSKNRKYFGTGTPFLTFKDVFDNLILP
                     NEFSGQVITEEKEREKYSVKKGDLFLTRTSEKQNELGISAVALKDYKNATFNGFTKRL
                     RPNKYCENKLLPVFAAFYFRSNNFRNQVNSMSIMSTRASLNNEMISKLKITIPSLQNQ
                     MKISHILLALLKKEKINQKIIANLEELSQTLFKRWFVDFEFPDENGNPYKSSGGEMVD
                     SELGEIPRSWKVDELGNYIKIKSGKRPKNKVDKEDIENVVPIIGASKIVGYTNDYLYN
                     EKIIIIGRVGTHGVIQRFSTRTWPSDNTFVITSDFES"
BASE COUNT          891 a          285 c          436 g          653 t
ORIGIN      
        1 aaagatgaat atttagcaga gaatattttc tgggttccta aagaatcaag atggcaatac
       61 ataaatgata attctaagaa gccagaaatt ggtcaaatta tcgataaagc tatgatagca
      121 attgaaagag aaaatgagtc tttaaaagga gtactaccta aagattatgc acgaccagct
      181 ttagataagg agaaattagg agatattatt gacttgttta ctttcaaagt cggagactct
      241 gaaagtaaga aacaagatgt cttggggcgt gtttatgaat acttcatcgc aaaattcgca
      301 agcgctgagg gtaagaatgc tggggaattc tatacaccag catcaatcgt aaaattgctt
      361 gttgaaatga ttgaaccata tcaaggtaga atttatgacc cctgctgtgg ttccggcggt
      421 atgtttgtac aaagtgaacg ttttgtagaa cgtcaccaag gacgcttaga taatattgcg
      481 gtatacggtc aagaatcaaa tccaaccact tggaaattag caaaaatgaa tttggctatt
      541 cgaggtatag ataatgattt aggtgatcat catgcagaca cgtttcataa tgatttacat
      601 aaagatttga aagctgattt tatattggct aatccaccat tcaatgcaag tgattgggga
      661 cgagaaaaat tactagatga ttaccgctgg aaatttggta ttccacctaa aggcaatgcc
      721 aactatgcat ggattgaaca tatgatttct aaacttgccc caagtggtac agcaggattt
      781 gtattagcga atggttcaat gtctacaagt ggtaaagatg aattagaaat acgtaaaaac
      841 ttaattgaac aagacttagt tgaatgtata gttacattac cagggcaact attctattca
      901 actcaaattc cagtatgttt gtggttcgtg acaaaaaata aagctaaaaa cggcaagaat
      961 gaacgacgtg gagaagtact atttatagat gctagaaata taggaagcat ggtatcaaga
     1021 acattaaaag aattctcaga tgatgaaata aaagatatag caaatgttta tcactcatgg
     1081 cgtggaacaa acaataacca atatgaagat aaagcaggtt tctgtaaagt agcaaagaca
     1141 gaagaaataa aaaacaatga atatatctta acgccgggac gttatgtagg attagcagaa
     1201 gtagaagaag actcagaacc atttgaacag aaaatggaaa gaatcacagc agatttaagt
     1261 gaacaatttg cgaaatcaaa agaactcgag gaccaaatcc gtaagtcatt ggagggatta
     1321 ggttatggag tttgaaacat ttaatttaac tgatttatat actattagtt caggtttatc
     1381 aaaaaataga aaatattttg gtactggtac acctttttta acatttaaag atgtattcga
     1441 taatttaata ttacctaatg agttttctgg gcaagttata actgaagaaa aagaaagaga
     1501 aaagtattca gttaaaaaag gagacctttt tttaacaaga actagtgaga aacaaaatga
     1561 gttaggtata agtgcagtag ctttaaaaga ctataaaaat gcgactttta atggatttac
     1621 caaaagatta agaccaaaca aatattgtga gaataaattg ttaccagtat ttgctgcatt
     1681 ttattttaga agtaataatt ttagaaatca agtaaattct atgtctatca tgtctacaag
     1741 agcaagttta aataacgaaa tgatttcaaa acttaaaatt actataccta gtcttcaaaa
     1801 tcagatgaaa attagtcata tactattagc acttttaaaa aaagaaaaaa tcaatcaaaa
     1861 aatcatagca aatcttgaag aactttctca aacactattc aaacgttggt ttgttgattt
     1921 tgaatttcct gatgaaaatg gaaatccata caaatcaagc ggtggagaga tggttgatag
     1981 tgaattgggt gaaataccta ggagttggaa agttgatgaa ttaggaaatt atataaaaat
     2041 aaaaagtggt aaaagaccta aaaataaagt tgataaagaa gatatagaaa atgtggtgcc
     2101 aataattggt gctagtaaaa tagtgggtta tacgaatgat tatttatata atgaaaaaat
     2161 tattataatt ggtagggtag gaactcatgg tgtaatccaa aggttttcta caagaacatg
     2221 gccatcagat aatacttttg taataacaag tgattttgaa agcat
//
LOCUS       DAHHYJ010000218         2236 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000218 DAHHYJ010000000
VERSION     DAHHYJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.224"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            45..>2236
                     /locus_tag="NBK22_002573"
     CDS             45..>2236
                     /locus_tag="NBK22_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding adhesin SdrG C-terminal
                     domain-containing protein"
                     /protein_id="HCD2415725.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNEATAPSENKTTEKVDSRQLKDNTQTVTADQPKVTMSDSA
                     TVKETSSNMQSPQNTTANQSTTQTSNVTTNDKSSTTYSNETDKSNLTQAKDVSTTPKT
                     TTIKPRTLNRMAVNTVSAPQQGTNVNDKVHFSNIDIAIDKGHLNKDTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDTFTFKYGQYFRPGSVRLPSQTQNLYNAQGNIIAKGIYDST
                     TNTTTYTFTNYVDQYTNVSGSFEQVAFAKRENATTDKTAYKMEVTLGNDKYSKDVIVD
                     YGNKKGQQLISSTNYINNEDLSRNMTVYVNQPKNKYTKETLVTNLTGYKFNPDAKNFK
                     IYEVTNQNQFVDSFTPDTSKLTDVTDKFKITYSNDNKTATVDLLNGQSSSDKQYIIQQ
                     VAYPDNSSTDNGKIDYTLETQNGKSSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANVGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDS"
BASE COUNT          921 a          323 c          390 g          602 t
ORIGIN      
        1 tattatattt atcaaatttt gatattttaa aggagtatta ttaaatgaat aataaaaaga
       61 cagcaacaaa tagaaaaggc atgataccaa atcgattaaa caaattttcg ataagaaagt
      121 attctgtagg tactgcttca attttagtgg ggacaacatt gatttttggg ttaagtggtc
      181 atgaagctaa agcggcagaa catacgaatg gagaattaaa tcaatcaaaa aatgaagcga
      241 cagctccaag tgagaataaa acaactgaaa aagttgatag tcgtcaacta aaagataata
      301 cgcaaactgt aactgcagat cagcctaaag tgacaatgag tgatagtgca acagttaaag
      361 aaactagtag taacatgcaa tcaccacaaa acactacagc taatcaatct actacacaaa
      421 ctagcaatgt aacaacaaat gataaatcat caactacata tagtaatgaa actgataaaa
      481 gtaatttaac acaagcaaaa gatgtttcaa ctacacctaa aacaacgact attaaaccaa
      541 gaacattaaa ccgtatggct gtgaacacag tatcagcacc acaacaaggt acaaatgtta
      601 atgataaagt tcatttttct aatattgata ttgctattga taaagggcac ttaaataaag
      661 atactggcaa aactgaattt tgggcaactt caagtgatgt tttaaaatta aaagcgaatt
      721 acacaatcga tgattctgtt aaagagggcg atacatttac ctttaaatat ggacagtatt
      781 tccgtccggg ttctgtaaga ttaccttcac aaactcaaaa tttatataat gcccaaggta
      841 atattattgc aaaaggtatt tatgatagta caacaaacac aacaacatat acttttacga
      901 actatgtgga tcaatataca aatgttagcg gtagctttga acaagttgca tttgcgaaac
      961 gtgaaaatgc aacaactgat aaaactgctt ataaaatgga agtaacttta ggtaatgata
     1021 aatatagtaa agacgtcatt gtcgattatg gtaataaaaa aggacaacaa ctcatttcaa
     1081 gtacaaacta tattaacaat gaagatttat cacgtaatat gactgtttat gtaaaccaac
     1141 ctaaaaataa atatacaaaa gaaacattag taacaaattt aactggttat aaatttaatc
     1201 cagatgctaa aaactttaaa atttatgaag taactaacca aaatcagttt gtggatagtt
     1261 tcaccccaga tacttcaaaa cttacagatg tcactgataa atttaaaatt acatacagta
     1321 atgataataa gacggcgaca gttgatttat tgaatggtca atctagtagt gataaacagt
     1381 acatcattca acaagttgct tatccagata atagttcaac agataatggg aaaattgatt
     1441 atactttaga aacacaaaat ggaaaaagta gttggtcaaa cagttattca aatgtgaatg
     1501 gctcatcaac tgcaaatggc gaccaaaaga aatataatct aggtgactat gtatgggaag
     1561 atacaaataa agatggtaaa caagatgcca atgaaaaagg gattaaaggt gtttatgtca
     1621 ttcttaaaga tagtaacggt aaagaattag atcgtacgac aacagatgaa aatggtaaat
     1681 atcagttcac tggtttaagc aatggaactt atagtgtaga gttttcaaca ccagccggtt
     1741 atacaccgac aactgcaaat gtaggtacag atgatgctgt agattctgat ggactaacta
     1801 caacaggtgt cattaaagac gctgacaaca tgacattaga tagtggattc tacaagacac
     1861 caaaatatag tttaggtgat tatgtttggt acgacagtaa taaagatggt aaacaagatt
     1921 cgactgaaaa aggaattaaa ggtgttaaag ttactttgca aaatgaaaaa ggcgaagtaa
     1981 ttggtacaac tgaaacagat gaaaatggta aataccgctt tgataattta gatagtggta
     2041 aatacaaagt tatctttgaa aaacctgctg gcttaactca aacaggtaca aatacaactg
     2101 aagatgataa agatgccgat ggtggcgaag ttgatgtaac aattacggat catgatgatt
     2161 tcacacttga taatggttac tacgaagaag aaacatcaga tagcgactca gactcagata
     2221 gcgactcaga ttcgga
//
LOCUS       DAHHYJ010000219         2157 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000219 DAHHYJ010000000
VERSION     DAHHYJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.225"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(208..753)
                     /locus_tag="NBK22_002574"
     CDS             complement(208..753)
                     /locus_tag="NBK22_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415726.1"
                     /translation="MKRKVLVLTMGVICATQLWHSNHANALVTESGANNTKQFTEIVS
                     EEKVIKDAISKVNESFIYYPQNDLKGLGGEHNDYEKITYSTSSNNVLELSMSSKYVGG
                     KSGAMVGYSEIDSSHFTDRDKRVIRRDHVKEAQSLVENYKDTQSADARMKAKQKVNTL
                     SKPHQNYFNKQIDKVYNGLQR"
     gene            complement(1087..2109)
                     /gene="emp"
                     /locus_tag="NBK22_002575"
     CDS             complement(1087..2109)
                     /gene="emp"
                     /locus_tag="NBK22_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCD2415727.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKNFVVPESGIN
                     KIIPTYDEFKSAPKVNVGSLADNKNFVASEDKFNKIADSSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNNHFITTQTHYKK
                     VITSYKSTHVHKHVNHATDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYRAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYYYSYKVVKGVKKYFSFAQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGS
                     LPAPRV"
BASE COUNT          642 a          331 c          337 g          847 t
ORIGIN      
        1 atattttagg tgccagaact tatatattta gccttatttg taaaattaaa aagcctacaa
       61 catgaatcgt tgcagactat catatcgaga caaagaggtt tattagcgtc tctatatcta
      121 tgcggaattt gaaaagatta tttattatat ttacggggat aaattgtcat ttaaaaacga
      181 gatgtataac ttataattta ctttggatta gcgttgtaat ccattataaa ccttatcaat
      241 ttgtttattg aaatagtttt gatgcggttt gcttaatgtg ttaacttttt gtttggcttt
      301 catcctagca tcagcacttt gtgtatcttt atagttctct actaagcttt gtgcttcttt
      361 aacatgatca cgtctaataa cgcgtttgtc acggtctgtg aaatgtgatg agtcaatttc
      421 actataacca accatagctc ctgatttacc gcctacgtat tttgaactca ttgataattc
      481 taaaacatta ttagaagaag tgctatatgt aattttttcg taatcgttgt gttctccacc
      541 taatcccttc aaatcatttt ggggatagta aataaagctt tcattgactt tcgaaatagc
      601 atcttttata actttctctt ccgatacaat ttcagtaaat tgcttagtat tatttgcgcc
      661 actctctgtc actaatgcgt ttgcgtgatt agaatgccat aattgagttg cacaaattac
      721 acccattgtt agtactagta ctttcctttt catttgttta tgctccctta tttgctttga
      781 ttgtaaaacc gactttttca ccctgttttc ttcgccattg aaaattatat accaatattt
      841 tagaaacaat aattaacttg cgatgaacaa actattaaca ataatcttga gtcttatatt
      901 tatcttaatt aataatatat ttatttaaga ttatgttact tccaactttc aaagtagaaa
      961 aacggtataa tttgttgata gatgtatatc acttagcgta aaaaatacag cctacaacca
     1021 catagattgt agactatatt taaaataata ggtatttatc atatctcgta aacttagtgc
     1081 caatttttat actcgtggtg ctggtaggct acccttaaat tcaggaacgt agtgtgtagg
     1141 gctataactt ggaacagcat attgataatt tacatttttg atatttaatg atggtttccc
     1201 aattttataa ccatttgatt gtgcaaatga gaaatatttc ttcacacctt taactacttt
     1261 ataagaatag tagtatttat agtcatatgc tttatttact ttagcatttt gatgtgttgc
     1321 tgttgtgtta ttttggaaac ttggtacatg catacgatgt gaattgtgac catatggtgg
     1381 aattaccttg aaactattta tttgtggcac aacacaaaag tgattaattt taatgctagc
     1441 atgccctggt gtaacaaatt tatgcgcgcg atatccagga actgcaaaat gatgcttgat
     1501 aattaaagat tgagatggat gtgtatatct aggcgattct gatggtttaa caataaagtg
     1561 tttattaata gaatcagttg catgatttac atgtttatgt acatgtgttg atttgtatga
     1621 agtaataact ttcttgtagt gggtttgcgt agtaatgaag tggttgttta ctttgttttg
     1681 cgtaataaat ctatttactt ctgggccttt agcaacaaaa tgcttatcaa cttgttgtga
     1741 agctggattg tttgttgcta cattcacgcg attattgatt tctttgtact ctggaacaat
     1801 gtttcctaac ttcgattctg gtacgacaaa gtttttatct acaattttac tagctgccga
     1861 tgaatctgca atcttattaa atttatcttc agaagctaca aagtttttgt tgtcagctaa
     1921 acttccaaca tttacttttg gtgcactctt aaattcatcg taagttggaa taattttatt
     1981 aatacctgat tctggaacta caaagttttt gtcaacactc tctgtcactg atgctttagc
     2041 gtgatttgaa ttcataattt gtgtagcaaa tagcgtgctc atagttaaaa ctaataattt
     2101 ctttttcatc tgttatttct cctttatata gactcaatat tataaccaat ttaattt
//
LOCUS       DAHHYJ010000220         2152 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000220 DAHHYJ010000000
VERSION     DAHHYJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.226"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            45..>2152
                     /gene="clfA"
                     /locus_tag="NBK22_002576"
     CDS             45..>2152
                     /gene="clfA"
                     /locus_tag="NBK22_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCD2415728.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSS
                     STNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLT
                     MPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFV
                     NPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRST
                     LYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSA
                     SDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDIDSDS"
BASE COUNT          776 a          381 c          434 g          561 t
ORIGIN      
        1 gtatatagtt aatataaaat gactttttaa aaagagggaa taaaatgaat atgaagaaaa
       61 aagaaaaaca cgcaattcgg aaaaaatcga ttggcgtggc ttcagtgctt gtaggtacgt
      121 taatcggttt tggactactc agcagtaaag aagcagatgc aagtgaaaat agtgttacgc
      181 aatctgatag cgcaagtaac gaaagcaaaa gtaatgattc aagtagcgtt agtgctgcac
      241 ctaaaacaga cgacacaaac gtgagtgata ctaaaacatc gtcaaacact aataatggcg
      301 aaacgagtgt ggcgcaaaat ccagcacaac aggaaacgac acaatcatca tcaacaaatg
      361 caactacgga agaaacgccg gtaactggtg aagctactac tacgacaacg aatcaagcta
      421 atacaccggc aacaactcaa tcaagcaata caaatgcgga ggaattagtg aatcaaacaa
      481 gtaatgaaac gacttctaat gatactaata cagtatcatc tgtaaattca cctcaaaatt
      541 ctacaaatgc ggaaaatgtt tcaacaacgc aagatacttc aactgaagca acaccttcaa
      601 acaatgaatc agctccacag agtacagatg caagtaataa agatgtagtt aatcaagcgg
      661 ttaatacaag tgcgcctaga atgagagcat ttagtttagc ggcagtagct gcagatgcac
      721 cggcagctgg cacagatatt acgaatcagt tgacgaatgt gacagttggt attgactctg
      781 gtacgactgt gtatccgcac caagcaggtt atgtcaaact gaattatggt ttttcagtgc
      841 ctaattctgc tgttaaaggt gacacattca aaataactgt acctaaagaa ttaaacttaa
      901 atggtgtaac ttcaactgct aaagtgccac caattatggc tggagatcaa gtattggcaa
      961 atggtgtaat cgatagtgat ggtaatgtta tttatacatt tacagactat gtaaatacta
     1021 aagatgatgt aaaagcaact ttgaccatgc ccgcttatat tgaccctgaa aatgttaaaa
     1081 agacaggtaa tgtgacattg gctactggca taggtagtac aacagcaaac aaaacagtat
     1141 tagtagatta tgaaaaatat ggtaagtttt ataacttatc tattaaaggt acaattgacc
     1201 aaatcgataa aacaaataat acgtatcgtc agacaattta tgtcaatcca agtggagata
     1261 acgttattgc gccggtttta acaggtaatt taaaaccaaa tacggatagt aatgcattaa
     1321 tagatcagca aaatacaagt attaaagtat ataaagtaga taatgcagct gatttatctg
     1381 aaagttactt tgtgaatcca gaaaactttg aggatgtcac taatagtgtg aatattacat
     1441 tcccaaatcc aaatcaatat aaagtagagt ttaatacgcc tgatgatcaa attacaacac
     1501 cgtatatagt agttgttaat ggtcatattg atccgaatag caaaggtgat ttagctttac
     1561 gttcaacttt atatgggtat aactcgaata taatttggcg ctctatgtca tgggacaacg
     1621 aagtagcatt taataacgga tcaggttctg gtgacggtat cgataaacca gttgttcctg
     1681 aacaacctga tgagcctggt gaaattgaac caattccaga ggattcagat tctgacccag
     1741 gttcagattc tggcagcgat tctaattcag atagcggttc agattctggc agtgattcta
     1801 catcagatag tggttcagat tcagcgagtg attcagattc agcaagtgat tcagactcag
     1861 cgagtgattc agattcagca agtgattcag attcagcaag tgattcagat tcagcaagtg
     1921 attccgactc agcgagcgat tcagactcag acagtgactc agattccgat agcgattccg
     1981 actcagacag tgattcagat tcagatagcg attcagattc cgacagtgat tccgactcag
     2041 atagcgactc agattcagac agcgattcag attcagacag cgattcagac tcagattcag
     2101 atagcgattc tgactcagac agtgactcag attcagatat cgattcagat tc
//
LOCUS       DAHHYJ010000221         2145 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000221 DAHHYJ010000000
VERSION     DAHHYJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.227"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            244..1176
                     /locus_tag="NBK22_002577"
     CDS             244..1176
                     /locus_tag="NBK22_002577"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCD2415729.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            1182..2021
                     /locus_tag="NBK22_002578"
     CDS             1182..2021
                     /locus_tag="NBK22_002578"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCD2415730.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYVENPSEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            2029..>2145
                     /locus_tag="NBK22_002579"
     CDS             2029..>2145
                     /locus_tag="NBK22_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD2415731.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKF"
BASE COUNT          745 a          285 c          424 g          691 t
ORIGIN      
        1 gccacctaat tccaatatat cattgtagag cttaggtcat tgatttatgg ctcggacttt
       61 tatggcgata tgaaccatgt aaattaagca agcaataaat taatgattga tattgacttg
      121 taaaataata acaataatga acaattaata tttattttag cttttcaatg tagattggtg
      181 ttatattttt gatatgataa gaagagatgt aagagtaggg ataaatacaa ttgaggtgaa
      241 cccatgttaa cgacagaaaa actagttgaa acattaaagt tagatttaat cgctggtgaa
      301 gaaggactat cgaagccaat taaaaatgct gatatatcaa gaccgggctt agagatggca
      361 ggttattttt cacattatgc gtcagataga atacaactat taggaacaac ggaactatcg
      421 ttttacaatt tattaccaga taaggatcgc gcaggtcgta tgcgtaaact atgcagacca
      481 gaaacgcctg caattattgt gacgcgtgga ttgcagccac cagaagaatt agttgaagct
      541 gcaaaagaat taaatacccc acttatagtt gctaaagatg cgactacaag tttaatgagt
      601 cgcttaacaa cgtttttaga gcatgcactt gcaaagacga catctttaca tggtgtttta
      661 gtagatgttt acggtgttgg tgtactaatt accggtgatt caggaatagg taaaagtgag
      721 actgcgttgg aattagttaa acgtgggcat agattagtag cagatgataa tgtagaaata
      781 cgtcaaatta ataaagatga actaataggg aaaccaccaa agttaataga acatctatta
      841 gaaatacgtg gactaggtat tatcaatgtt atgactttat ttggcgcggg ttcaatatta
      901 actgaaaaac gaattagatt aaatattaat ttggaaaact ggaacaagca aaagttatat
      961 gaccgcgtag gtcttaatga agagacgcta agtattttag atactgaaat cactaaaaaa
     1021 acaatacctg taagacctgg tagaaatgtt gcggtaatta ttgaggtcgc tgcaatgaac
     1081 tatcgattaa atatcatggg cattaacact gccgaagaat ttagtgaaag attaaatgaa
     1141 gaaattatca agaacagtca taagagtgag gagtaggttg aatgggtatt gtatttaact
     1201 atatagatcc tgtggcattt aacttaggac cactgagtgt acgatggtat ggaattatca
     1261 ttgctgtcgg aatattactt ggttactttg ttgcacaacg tgcactagtt aaagcaggat
     1321 tacataaaga tactttagta gatattattt tttatagtgc actatttgga tttatcgcgg
     1381 cacgaatcta ttttgtgatt ttccaatggc catattacgt ggaaaatcca agtgaaatta
     1441 ttaaaatatg gcatggtgga atagcaatac atggtggttt aataggtggc tttattgctg
     1501 gtgttattgt atgtaaagtg aaaaatttaa acccatttca aattggtgat atcgttgcgc
     1561 caagtataat tttagcgcaa ggaattggac gctggggtaa ctttatgaat cacgaggcac
     1621 atggtggacc tgtgtcacgc gcttttttag aacaattaca tttgcctaat tttataatag
     1681 aaaatatgta tattaacggc caatattatc atccaacatt cttatatgaa tccatttggg
     1741 atgtcgctgg atttattatt ttagttaata ttcgtaaaca tttaaaatta ggagaaacat
     1801 tctttttata tttaacttgg tattcaattg gtcgattctt tatagaagga ttacgtacag
     1861 atagcttaat gctcacaagt aatattagag ttgcacaatt agtatcaatt cttttaattt
     1921 taataagtat aagtttaatt gtatatagaa ggattaagta taatccaccg ttgtatagca
     1981 aagttggggc gcttccatgg ccaacaaaaa aagtgaagta gtgatatttt gagaaaattt
     2041 ttatcaaaaa cacatcatca cacaaaccct ttatggcgtg tataccgtct tgttaaattt
     2101 tcgaaagttt ttaagaatgt aattatcatt gaattttcga aattt
//
LOCUS       DAHHYJ010000222         2109 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.228, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000222 DAHHYJ010000000
VERSION     DAHHYJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.228"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            104..322
                     /locus_tag="NBK22_002580"
     CDS             104..322
                     /locus_tag="NBK22_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCD2415732.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            322..2019
                     /locus_tag="NBK22_002581"
     CDS             322..2019
                     /locus_tag="NBK22_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD2415733.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
BASE COUNT          744 a          330 c          367 g          668 t
ORIGIN      
        1 aattatagag ataattggaa tgaatttatc gatatatact ccaatacgat tttactaggg
       61 ttaacaataa attaaacaaa cattcttagg aggaattttt aacatggcag tatttaaagt
      121 tttttatcaa cataacagag acgaggtaat tgtgcgtgaa aatacacaat cactttatgt
      181 tgaagctcaa acagaagaac aagtacgtcg ttacttgaaa gatcgtaatt ttaatatcga
      241 atttatcact aaattagagg gcgcacattt agattacgaa aaagaaaact cagaacactt
      301 taatgtggag attgctaaat aatgaaacaa ttacatccaa atgaagtagg tgtatatgca
      361 cttggaggtc taggtgaaat cggtaaaaat acttatgcag ttgagtataa agacgaaatt
      421 gtcattatcg atgcaggtat caaattccct gatgataact tattagggat tgattatgtt
      481 atacctgact acacatatct agttcaaaac caagataaaa ttgttggcct atttataaca
      541 catggtcacg aagaccatat aggcggtgtg cccttcctat taaaacaact taatatacct
      601 atttatggtg gtcctttagc attaggttta atccgtaata aacttgaaga acatcattta
      661 ttacgtactg ctaaactaaa tgaaatcaat gaggacagtg tgattaaatc taagcacttt
      721 acgatttctt tctacttaac tacgcatagt attcctgaaa cttatggcgt catcgtagat
      781 acacctgaag gaaaagtagt tcataccggt gactttaaat ttgattttac acctgtaggc
      841 aaaccagcaa acattgctaa aatggctcaa ttaggcgaag aaggcgttct atgtttactt
      901 tcagactcaa caaattcact tgtgcctgat tttactttaa gcgaacgtga agttggtcaa
      961 aacgtagata agatcttccg taattgtaaa ggtcgtatta tatttgctac cttcgcttct
     1021 aatatttacc gagttcaaca agcagttgaa gctgctatca aaaataaccg taaaattgtt
     1081 acgttcggtc gttcgatgga aaacaatatt aaaatcggta tggaacttgg ttatattaaa
     1141 gcaccacctg aaacatttat tgaacctaat aaaattaata ccgtaccgaa gcatgagtta
     1201 ttgatactgt gtactggttc acaaggtgaa ccaatggcag cattatctag aattgctaat
     1261 ggtactcata agcaaattaa aattatacct gaagataccg ttgtatttag ttcatcacct
     1321 atcccaggta atacaaaaag tattaacaga actattaatt ccttgtataa agctggtgca
     1381 gatgttatcc atagcaagat ttctaacatc catacttcag ggcatggttc tcaaggtgat
     1441 caacaattaa tgcttcgatt aatcaagccg aaatatttct tacctattca tggtgaatac
     1501 cgtatgttaa aagcacatgg tgagactggt gttgaatgcg gcgttgaaga agataatgtc
     1561 ttcatctttg atattggaga tgtcttagct ttaacacacg attcagcacg taaagctggt
     1621 cgcattccat ctggtaatgt acttgttgat ggtagtggta tcggtgatat cggtaatgtt
     1681 gtaataagag accgtaagct attatctgaa gaaggtttag ttatcgttgt tgttagtatt
     1741 gattttaata caaataaatt actttctggt ccagacatta tttctcgagg atttgtatat
     1801 atgagggaat caggtcaatt aatttatgat gcacaacgca aaatcaaaac tgatgttatt
     1861 agtaagttaa atcaaaataa agatattcaa tggcatcaga ttaaatcttc tatcattgaa
     1921 acattacaac cttatttatt tgaaaaaaca gctagaaaac caatgatttt accagtcatt
     1981 atgaaggtaa acgaacaaaa agaatcaaac aataaataat caaaaagcta ctaactttga
     2041 agtgaagttt taattaaact caccacacat tgttagtagc tttttcttta tatatgatga
     2101 gcttgagac
//
LOCUS       DAHHYJ010000223         1976 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000223 DAHHYJ010000000
VERSION     DAHHYJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.229"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            295..1086
                     /locus_tag="NBK22_002582"
     CDS             295..1086
                     /locus_tag="NBK22_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCD2415734.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
     gene            1101..1745
                     /gene="trmB"
                     /locus_tag="NBK22_002583"
     CDS             1101..1745
                     /gene="trmB"
                     /locus_tag="NBK22_002583"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCD2415735.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK"
BASE COUNT          717 a          257 c          385 g          617 t
ORIGIN      
        1 gaaaatggga tagcaattat tttgaaaact agagtttatc gcctacagag attcaaaaac
       61 atgtagtttt agacacggta acattatatg tcaagtaagt gactatagaa tgtatggtgt
      121 caatgacaga cattacgatt taaatttagt cgtgtaatgt aggcattggt tgaggattta
      181 caataattat ctggacataa tatttgtacc agtatggtgt tataaattag aatatatggc
      241 tcttgaaaaa cctatttcta gagccatttt ctaattgaaa gtgaggtgga cgagttggag
      301 cagttttatc aattagggtg gacacttgat tcagcaggtg gtgcatctgg tgaagcatat
      361 atggctgaac aagatggaca aaagttgttt ttaaaacgaa attcaaatcc atttattgcg
      421 gcattatcag cagaaggtat tgtgcccaaa ttagtatgga cgaaacgcat agaaacaggc
      481 gaggttgtta cagcacaaca ttggaaaaat gggcgtgaac tatcttcaaa cgaaatgaag
      541 caaacaagag ttgcacattt attaaagaag atacacaatt ctagaccttt attaagtatg
      601 ttaaagcgta tggaaatgga acctattact cctgagatta tgcttaataa aattaatgcc
      661 tctttatcaa gagaagtttt aacacatcat attgtgagaa aatcattaac ctatttagaa
      721 gagcatatac cgagtttaga ttcgcgtttt ttcactgttg tacatggtga tgtgaatcat
      781 aataattggt tattatccga tcgagatgaa ctatttttag ttgattggga aggtgcaatg
      841 attgcagacc cggcaattga tatcggaatg ctgctttaca actatgttcc acaacaacaa
      901 tggtctgaat ggcttgaaac atatggtgtt caagagagct tgaatttaaa taaacgtatg
      961 aaatggtata cggtaattca atctataggt cttgttcaat ggtatgaaga gcaaaaaaga
     1021 tataaggata tgaacacttg gcttaaattc ctaaatgaag ttatgaatag taacatgttt
     1081 atttaagagg agaataaaat atgagagttc gatacaaacc atgggctgaa gattatttaa
     1141 aagatcatcc tgaattggtt gacatggacg gtcaacatgc agggaaaatg actgaatggt
     1201 ttgataaaac gcaaccgata cacattgaaa ttggttcggg tatggggcag tttattacaa
     1261 cattagctgc tcaaaatcct catattaact atatttcaat ggaacgtgag aaaagtatcg
     1321 tttataaagt attggataaa gttaaagaga tgggtttaac aaatttaaag attatttgta
     1381 atgacgccat tgaattaaat gaatatttta aagacggcga agtttcgcgt atttatttga
     1441 acttttcaga tccatggcca aaaaatcgtc atgcaaaacg acgtttaacg tatcatacgt
     1501 ttttagcgtt gtatcaacaa atcttaaatg atgaaggaga tttacatttt aaaactgata
     1561 acagaggact atttgcctac agtttagaaa gtatgtctca atttggaatg tattttacaa
     1621 aaataaatct aaatttacat caagaagacg atggcagtaa tattttaaca gaatatgaaa
     1681 agaaattttc ggataaaggg tcacgtattt atcgtatgga agcgaaattc cattcacaaa
     1741 aatagagcac ttatgagggt atgtacacat acaatcataa gtgttttttc gtactataat
     1801 ggttctatag ataaaggttc tataataaat cggactgatg gaaaaagttt ttaacttttc
     1861 atctgtccga ttttttgatt ttgaatataa aaaaagcgca attatctcta taattaaaag
     1921 tgactaaaca aataactaaa ggagataatg cgcctatgaa caatgttata ctaaat
//
LOCUS       DAHHYJ010000224         1949 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000224 DAHHYJ010000000
VERSION     DAHHYJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.230"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            75..548
                     /locus_tag="NBK22_002584"
     CDS             75..548
                     /locus_tag="NBK22_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCD2415736.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSKCNCPHADGRRVICKHMIALLFTASPEAANKHIMMLNEVEEDY
                     QLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEEEMFEDEFY"
     gene            complement(596..1363)
                     /locus_tag="NBK22_002585"
     CDS             complement(596..1363)
                     /locus_tag="NBK22_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD2415737.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDRLLQLQHDGHELIFASARPI
                     RDLLPVLPSVFHQHTLIGANGAMISQQSKISVIKPIHTDTYHHILKIIQKYELDYIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKIILLNIDPAQITTILDELNK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIFMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNTQAICKVLKSYK"
BASE COUNT          680 a          273 c          311 g          685 t
ORIGIN      
        1 caagtatatt aatctagaca cttatcgatt ttattttctt tatttaaaaa taataataaa
       61 aaggagtatc attaatggga ttacttgata ttgcaagtat tcgttctata gaaagaggct
      121 ttaattatta tcaaagtgaa tgcgtcatta acttaaaatc attttcagaa acgcagcatg
      181 aggctgaagt aaagggcagt ggcaacaaag tatatcgttg ttatattgat atggaacatc
      241 ctagaaaatc caaatgtaat tgtcctcatg ctgatggaag acgagtgata tgtaaacata
      301 tgattgcatt actctttaca gctagtccag aagcagcaaa taaacatata atgatgttaa
      361 acgaagttga agaagactat caattacgca gaaatatgtg gattgatagt cttaaagaaa
      421 tgattaatga tatgagtgaa gaagaactcc gcgatgcata tttaaacatg ttaattgaac
      481 atggagaaat ggcagaatta tttggattag atgaagagga agaaatgttc gaggacgaat
      541 tttattaaaa tagcccctat cgattgataa tgattatcat ttgatagggg tgcttttatt
      601 tatatgattt taagactttg caaatagctt gtgtattatt attgatgtgt ttgattttat
      661 caagtttcaa tattgcgtgt gtgtatgctt ctgatggtcc tataatatag ccactactag
      721 catgttgtaa catgaaaata tcattgtggt cattaccaaa tgctatatat ttaacatctg
      781 catcaaatat atattgtaat gcagtatatt tattaatatt ttgcgctgtt atatcaatgt
      841 tatactcatt tgaatggtga atcatttcca attcttgatg gtatttattt agctcgtcta
      901 atatagttgt aatttgtgcc gggtctatat ttaataaaat gatcttgatt ggtgtgtcga
      961 tatttgcaac atcaatacaa ctggccagct tatgtggatc taaacgctca aaaatcgcgt
     1021 tctcagcgtc aagttgtgca gcataattcc aatcatcatc aataatataa tctaactcat
     1081 acttttgaat tatttttaag atatggtgat atgtatctgt atgaattggt ttgataacag
     1141 aaatctttga ttgctgtgaa atcatagcac catttgcacc aattaatgtg tgctgatgaa
     1201 atactgatgg taaaactggc aacaaatcac gaatcggacg tgctgatgca aatataagtt
     1261 catgaccatc atgttgtaat tgtaacaatc tatcaataat agtctggtca attaatcggc
     1321 cgtcaaaaca aagcgtacca tcaatatcaa agacaaatct catcaaatca ctccaaacaa
     1381 tataatacca tgattatagc ataagtaagt tatgtgacga tattagataa tgattatgat
     1441 gtagggtacc ttttgcgtca cacacattac ttcctataat atatattatg taaactagaa
     1501 tgtgaaattc cataagggga cttatataac tgtatgtttg tgtagtgttt atgtcagtaa
     1561 gctaaattta cattcatgtt atgtttcatt aaaccaatta ctcacgtttt ggtgcatatt
     1621 aaatctttta tatcgatcat acatctatca tcatttttat ttctaaaata caaactgaat
     1681 actttgctag aatttgttac agcaatcatt gctcatgcat tttataaatt acaattagac
     1741 aaatatgaca tttgatatca cacacttgca aacacacaca tatataatca gacataaatt
     1801 gttatgctaa ggtttattca ccaaaaatat aatacatatt ggcttgtttt gagtcatatt
     1861 gaatgaatta aaaagtatac tcaactcaat catttacaaa taggttgtgc aattcatatt
     1921 tatatttatt gcaatcactt gttaaactt
//
LOCUS       DAHHYJ010000225         1927 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.231, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000225 DAHHYJ010000000
VERSION     DAHHYJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.231"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            192..896
                     /locus_tag="NBK22_002586"
     CDS             192..896
                     /locus_tag="NBK22_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD2415738.1"
                     /translation="MSKNINQANSKLNTSNKKLKQAYSKSDSKNMKVIYMPHWLEFYS
                     IAIHSDVTSNKKRYYKSYSRGTVVYVKLGSNIGSEFSGNHFCVILDNKDNKGKETVTI
                     VPLSSKGNKNYLKLNESVLNLTATDLKKQIIDISSKVQALAQKYREIDIKLSTEDKKL
                     LSNLNQKANELYRVIGVYSKHKGKDTYVNISAITTISKRRISKINDSDPTGTIIISED
                     DMNEIEKQLKIKFFSN"
     gene            1289..1825
                     /locus_tag="NBK22_002587"
     CDS             1289..1825
                     /locus_tag="NBK22_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415739.1"
                     /translation="MHNNNEFNKKLKDFIGSDKRMALVKGYVNEYKLTTVLRALNNSD
                     YTKGTIHTRSIGNLKQIVDNRIIPKNITQNTIFKLSNLTLEVNLYERNTIYHGDFAIY
                     YPVESALIIETDTPRLINHINNNPVQKIFIITTNDWSFNTLELEKIVDETIIYDLKQE
                     DPKQYEILYKNKHGKLPY"
BASE COUNT          807 a          296 c          273 g          551 t
ORIGIN      
        1 acatacatga actataaaaa gcgaataaat agcaggtcat tgcgaaattt gctaaataca
       61 aaattgatga gttccaccta ataaaaattg ttggttttat agtaacatct attttcgtac
      121 cattcatgtt aatttcagtg ctaataatgc tgatataaag aagaataaat tgattaggag
      181 aacactattt aatgtctaaa aatatcaatc aagcaaatag taaattaaat acgtcaaaca
      241 aaaaactaaa acaagcttat tcaaaatccg acagtaaaaa catgaaagtt atatacatgc
      301 ctcattggct agagttttat tcaattgcta tacatagcga tgttacaagc aataaaaaaa
      361 gatattataa atcatatagt agaggaacag tggtatatgt aaaactaggg agtaatattg
      421 gaagtgagtt ttctggtaat catttttgtg taatattaga taacaaagac aataaaggaa
      481 aagaaacagt tacaatagtt ccactttctt ccaaaggtaa taaaaattat ttgaagttaa
      541 acgaatcggt gctaaatcta acagcaacag atttaaaaaa acagattatc gatatcagta
      601 gtaaagtaca agcattagcc caaaaatata gagaaattga cattaaactt agcacagaag
      661 ataaaaagct tttgtctaac ttaaatcaaa aagctaatga actgtataga gtgataggtg
      721 tttattccaa gcacaaaggg aaagatacat acgttaatat atcagcaatt acaacaataa
      781 gcaaaagacg tataagcaaa ataaatgatt ctgatcctac tggaacgata ataatttcag
      841 aagatgacat gaatgaaatc gaaaagcaat taaagataaa gttcttttct aattgaattg
      901 taatattaca tctgatataa ttgatttaca aattcccggt aaccattccg gcttcattct
      961 cttgaagaat gacatattct cagcgtttta gctgaaggtc agatgatacg tcatctggcc
     1021 tcttttttta tataaatgcc ctttaagtga aatatacaac aatctgcaat caatattaga
     1081 catcacaaaa cacacaataa attacacaat gtgttataag ccacatgtca ctattatcgc
     1141 agctagctgc gtcactcaaa acgaatacgg aacgcgttgg ttatttcaat cataatatta
     1201 ctctgcaaat acacagccct gtaaccgagt aaatggatag agatttgaac aaatgaaaac
     1261 aatcaactaa tggaaaggat aaaatattat gcacaacaat aatgaattca acaaaaaact
     1321 gaaagatttt ataggtagcg ataaacggat ggctttagta aagggttatg ttaacgagta
     1381 taaattaaca actgttttaa gagctttaaa taattccgat tacacaaaag ggacgataca
     1441 cactcgttca ataggcaacc taaaacaaat agtagataat cgaattattc ctaaaaatat
     1501 aactcaaaac acaatcttca aattgagtaa tttaacctta gaggttaacc tctatgaaag
     1561 aaacacaatt tatcacggag actttgctat ttactatcct gtagaatctg ctctaataat
     1621 agaaacagat actcctaggc taattaatca tataaataac aacccagtcc aaaaaatatt
     1681 tataataaca acaaatgact ggagctttaa tacacttgaa ttagaaaaaa tagtagacga
     1741 aactataatt tatgatttaa aacaagaaga tcctaaacaa tatgaaattt tatataaaaa
     1801 taaacacgga aaattgcctt attgatgaat taaaacgcaa acttagaaca ccttacccct
     1861 tttccgtcct tattgtaatt ttccaactac tcaaattaaa aaccacccgt gcaaacaggt
     1921 ggtttta
//
LOCUS       DAHHYJ010000226         1913 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000226 DAHHYJ010000000
VERSION     DAHHYJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.232"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(395..856)
                     /locus_tag="NBK22_002588"
     CDS             complement(395..856)
                     /locus_tag="NBK22_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCD2415740.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(881..1792)
                     /locus_tag="NBK22_002589"
     CDS             complement(881..1792)
                     /locus_tag="NBK22_002589"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCD2415741.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
BASE COUNT          713 a          333 c          252 g          615 t
ORIGIN      
        1 taaaaaagat atattaaaaa tccaactaaa aataatacac cgacgactct tataattaac
       61 tttttcatca atcaaccacc taaaaagtat taatactatt gtaaaaaaca acacattaat
      121 tagcaaattt tcaacactga cataactgtg tcgtttcgat gaaacaaaat catcttcagg
      181 cataatttta acgtcataaa gattttccct acactctata tcataaccta tctttatgtt
      241 ttcaggttga atttcattat taggattaaa atatgtataa aaaaatggac agttaaggta
      301 tcaaatgaat accatcaact gtccgactat tcttcatcaa aaaacctgat aaaacaaatt
      361 gccttatcag attagtatca tttgtataag catattaatg accaaatgtt gctttaatca
      421 gtgatgtcgt ttctccacct ggatataata catataataa taagtaaact gctacacctg
      481 taattgcagt acagaaccat ataactgaag cgaatggccc gaatttgcgg tgtacattat
      541 atttatcttt aaatgcagta ataatttgaa ctaggcctag aataccacca attgttgcta
      601 aattaatgtg gaaaaataag aaaatcgtat aatatttctt aattgatgct ggtccgccaa
      661 atgctgtatt accgatgaaa atcgttctac ttgcatagat taagaaaaaa gttaaagcaa
      721 aaacagctgc agctaacata acatttttgt gtttatttat ttcccttttc caaataagtc
      781 tccaaccaat ggcaattaaa attgcactaa tgacaataca tgtcgtacta atcgttggta
      841 aaattggaac gcccatattt ttcatcctaa cttaattaat ctagatcaaa gtaagtaatg
      901 aaacaatcac agctaacacg aaaaagatca ctaaataatt tagtgaatat ataaacattt
      961 gtgttgccca ttttgtttga tctgaatttt tcttaaatgt tgttaaacct aatgcaatcc
     1021 atcctaaatt taataatgta gctaacacta cgaatacgac acctaaattt attagtaata
     1081 aaggtactgg caataaaata atcaaccaga taaacatact gacacgtgta cgtttaaagc
     1141 ctttaactga tggtaacatt ggaatatttg caagtgcata ttcatcttta cgtttaatag
     1201 ctaaggcata aaaatgaatt ggttgccaac aaaatacaac taaaaacagc gcaatcgctg
     1261 ttaaactaat ttgtccttca attgcaaccc atccaattag tggtggtact gctccaggaa
     1321 aactcccaat cactgtgttc catgttgtat gtcttttaga ccatattgag taataagaca
     1381 cataacctac aatccccata agaccaagta cgcctgatgg tatattcaat aaaaacaaac
     1441 aaatttctcc aactaacatc ataccaaaac ttaataataa taaattttga tctgtaattc
     1501 tattatttac agttggtcta ttttgtttac taggcataat acgatcaata tcttggtcgt
     1561 aataattatt taacgcacat gcgccaccca taattaaagt agatcctaat agcattaata
     1621 aaatttgagg tattgatgat aagaaggaat gatttgtcat tacaactgct agccatgcgc
     1681 ccgcaaaagc cggaattaag ttaccttgaa caagtcccat tttaattatc tgttgcaatt
     1741 ctttgaagtt aactctgcta atattttgtg acaaagtatg ctctttgctc ataatccccc
     1801 tccttaaatt tgttcatata agattatgat atcttagatt gcataaaaag actaggttta
     1861 ataaaattaa attgtgacaa attaacgaca agagaaaatg tcgattttgt gac
//
LOCUS       DAHHYJ010000227         1826 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.233, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000227 DAHHYJ010000000
VERSION     DAHHYJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.233"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(293..1222)
                     /locus_tag="NBK22_002590"
     CDS             complement(293..1222)
                     /locus_tag="NBK22_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD2415742.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIILNIIINPEVFTIHFYNNQSFNYTWVVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIVGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSIALLILTAIFNRSL
                     KLKTSHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(1417..1641)
                     /locus_tag="NBK22_002591"
     CDS             complement(1417..1641)
                     /locus_tag="NBK22_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCD2415743.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNVVTEQVVRDSMNALVDLN
                     IIKSNSGTITKVHSAQIIDKTTTVLFEDKK"
     gene            complement(1658..>1826)
                     /locus_tag="NBK22_002592"
     CDS             complement(1658..>1826)
                     /locus_tag="NBK22_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415744.1"
                     /translation="FTKVDANGKQTEFKRRFANINPDASNDQIKTFSKLIERLTGETY
                     SNIELIKSLSI"
BASE COUNT          682 a          255 c          273 g          616 t
ORIGIN      
        1 tgaattataa aaagttgtgc attttgactt atttttaatt aattgaataa ccaactatga
       61 aatattagca cgaataacat aatttttgct attaatttgg tgtaaattat taaaaaataa
      121 cattaattta ggtcttaaga atgagttgac tatcgaattt gctaaattaa aatttgttgt
      181 gtcaaagcat actacccaaa tcaacgcttt tctaaagatt tttagaccac aaaaaaatag
      241 cttatcactt ttaaaaataa ttacaagtta aaatgataag ctaaaagtta atttaaaata
      301 atcttaaaag tataatgcct gtcaaaatac ataatagacc aatagttttt ctggatgtca
      361 ttgctatttt aggtgaacca aataatccaa agtgatctat caatatgccc attagaatct
      421 ggccaaacat cccaataagt gttgttaatg ctgcacccat atgaggcatt aagataatgt
      481 tagctgttac aaaagccatg ccaagtatac cgccagtaaa atagataggc tttaatttac
      541 cgaattttaa atgacttgtt tttagtttta aagaacgatt aaaaatagcg gttaaaatca
      601 atagcgctat tgacccaatt gtaaatgata ctaatgatgc aaaggctggt gaatgagtat
      661 gactagctaa agcactatta attgtcgttt gaataggtgg aaagaaacca aaaataaatc
      721 ctaatagaag ccaaaacagt aaatactttt gatcagttag taataaatta ttcttgttaa
      781 attgattcat tatgacgatg ccgacaatga gtaacaatac tccaattgct ttaattaaat
      841 taaaatcatg aattgtagcg ccaaataatc caaatgtatc aataatgaca cccataataa
      901 tttgacctgc aactgttgca attacagtta atgttgcacc taattttggc aataacaata
      961 aattgccagt taaaaagcta accccaagca aaccaccgac tacccatgtg tagttaaatg
     1021 attgattatt gtaaaagtga atagtaaata cttctggatt gataatgata tttaaaataa
     1081 ttaaacaaat tgttccaact gaaaatgaaa tgaatgaagt atagaaaggc gatttagtat
     1141 acagtgatag tcttgaattg acagatgttt ggataggaat aagcattcca actaggacac
     1201 caatgatata gaaaagaagc atatgaataa agtctcactt tccaaagtta ataataaaat
     1261 catatattta cattatcatt atagagaaaa tatacaactc atttacaata atgttgaaaa
     1321 aaacaggaca ttattacaac aaaatcaccc tagacaatca tgatgaaagt ctagggtgtt
     1381 agtatttata ctaaattaat tgcgttttct ttatatctat ttcttatctt caaataaaac
     1441 agttgttgtt ttatcaataa tttgagcaga atgtactttg gtgatagtac cactatttga
     1501 ctttataata tttaaatcta ctaatgcatt catactatca cgtacaactt gctcggtcac
     1561 tacgttgttt aattgtggta attgcagttt tacaggcttg tctagtgaag atttaaaact
     1621 taattcaagt gttttagtca tgaatatgtc ctcctgatta aattgataaa gatttgatga
     1681 gttcgatatt gctatatgtt tctccagtaa gtcgctcaat aagtttgctg aatgttttaa
     1741 tttggtcgtt tgatgcatca gggttaatgt tagcgaatcg acgcttaaat tctgtttgtt
     1801 tgccgttagc gtctacttta gtaaat
//
LOCUS       DAHHYJ010000228         1786 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000228 DAHHYJ010000000
VERSION     DAHHYJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.234"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..214
                     /locus_tag="NBK22_002593"
     CDS             <1..214
                     /locus_tag="NBK22_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCD2415745.1"
                     /translation="LSPGGHNENTFKTILSGMINVINGLDEVRNEYGDAHGKSKKNYR
                     PETRHAFLAINAARTITEFLLASYKK"
     gene            339..1610
                     /locus_tag="NBK22_002594"
     CDS             339..1610
                     /locus_tag="NBK22_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HCD2415746.1"
                     /translation="MDNHIKIFDNLFQSNISKFQNLSSKSYIIRNDTEKNSYLPMVQE
                     IRELFGKEGEIFSKSIGTHPDFFEIENTNEYQYICSLFVDISGSTKLALKYSLDKVKL
                     YKNAIISSAIEIFRAFDGHIHRIQGDAVLVYFGHKELEKSDAIINAINAASLMQYFNA
                     TTLKKFFESENLEPLKIRIGIDFGDDSSVLWSKYGIDGINEITSTSIHTDLASKLQNK
                     APSNKIMIGENINKYLDIPTKFRSIKTEKNNGVDVEKRYILNTNNLGRYSMEVFEWEK
                     YLNSFSMLPPFSTENEQFYSPRDLKIRCWIIDEKNQDKYEYIERGSALKKEMNLLFKL
                     EIYNQCLEFKNIKWRVVNYGEEAKKDKELEFEMNQYEGYQYCNLKTAYTGLHFMECYL
                     YDINDKIICHDSFGLFINDNNREVRKLGIED"
BASE COUNT          716 a          191 c          311 g          568 t
ORIGIN      
        1 cctttctcct ggtggacata atgaaaatac ttttaaaaca attttgagtg gaatgataaa
       61 tgtaattaac ggtctggatg aagtaaggaa tgaatatggt gacgcacatg gtaaatcaaa
      121 gaaaaattat aggcctgaaa ctagacatgc ttttctagca atcaacgcag cacgtacgat
      181 aactgaattt cttttagctt catataaaaa gtaaaagcta tttgatgaat agcaatcaaa
      241 tatattggtt ataaaaaacc gcatcatcaa ctgataagca gaggcgtatc ataagtagaa
      301 gggggattag ccaatttaat aaataaaggg gtgacagaat ggacaatcac ataaaaattt
      361 ttgacaattt atttcaaagt aatatttcaa aattccaaaa tctttctagt aaatcataca
      421 ttattagaaa tgatactgaa aagaatagtt atcttcctat ggttcaagag ataagagagt
      481 tgtttggaaa agaaggagaa attttttcta aaagtatagg aacacatcct gacttttttg
      541 aaatagaaaa cactaatgaa taccagtaca tttgttccct ttttgtagat atatctgggt
      601 ctacaaaact agctttgaaa tatagtttgg ataaagttaa actttataaa aatgcgataa
      661 tttctagtgc tatagaaata tttcgtgcat ttgacggtca tattcataga atacaaggag
      721 atgcggtgct tgtttatttt ggacataaag aactggaaaa aagtgatgca ataattaatg
      781 caatcaacgc tgcgtcatta atgcagtatt ttaacgccac aacattaaaa aaattttttg
      841 aaagtgaaaa tttagagccg ttaaaaatta gaataggaat tgattttggt gatgattcta
      901 gtgtattgtg gtcaaaatat ggaattgatg gaattaatga aataacttct accagtatac
      961 atactgactt agcatcaaaa ttacaaaaca aagccccttc aaataagatt atgataggag
     1021 aaaatataaa taaatatctt gatattccta caaagtttag aagtataaaa actgagaaga
     1081 acaatggtgt tgatgttgag aaaagatata ttttaaatac aaataatttg ggaagatatt
     1141 ctatggaagt gtttgaatgg gaaaaatact tgaatagttt ttcaatgttg cctccttttt
     1201 ctacagaaaa tgaacaattt tactcaccta gagatttgaa aataagatgt tggattattg
     1261 atgaaaaaaa tcaggataaa tatgaatata ttgaaagagg tagtgcttta aaaaaagaaa
     1321 tgaatttgtt atttaaatta gaaatttata atcaatgtct agaatttaaa aatattaaat
     1381 ggagagtggt aaactacgga gaggaggcaa aaaaggacaa ggaattagaa tttgaaatga
     1441 atcagtatga agggtatcag tattgtaatc taaagacagc ttatacagga ttacatttta
     1501 tggaatgtta tttgtacgac atcaatgata aaataatttg tcatgatagt tttggtcttt
     1561 ttataaatga taataataga gaagtgagaa aattaggaat agaggattga gtaatgtgaa
     1621 aaaagatgag ttcataaagt tacatcaaag ctatctaaat gaatatatta aatttgctga
     1681 tgccaaggca ttagctatca taagtattaa tggctttatt ttaaatttta acttttcaaa
     1741 acgtaatatt aaatttcata ataccgaaga cattttcaat tttaca
//
LOCUS       DAHHYJ010000229         1777 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000229 DAHHYJ010000000
VERSION     DAHHYJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.235"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            387..1550
                     /locus_tag="NBK22_002595"
     CDS             387..1550
                     /locus_tag="NBK22_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD2415747.1"
                     /translation="MLKHKIINLIQEKREGSYWDFKAEYHKDKAELLHDIICLSNNLL
                     NQEAYLILGVADNGHILGVAGDSNRKNQEELISFITGKKFAAGRHPKISLMTFEYEEK
                     EIDVIIINPKGYVPYYLEKAETDQKSKKNKTVNAGSIYTRVEDKNTPIDSTASPLDTE
                     ILWKMHFGLYPTPIKRLQNYLLTPEKWMQNSTGYFYSESPEYIVYKNEDIEEKENYFN
                     LVSPFYAYNQINSNTLYSYYEFKYHSTVLYACRCISLDSGIYTTPVPKSGEINFNMHR
                     DDTIYYRYFIEETMLYNIHLFMYKGDSMEEKFAMEKFLECVLVYKNEEEKELFENYIL
                     HNWDKVNQSINENNKRVFGTEHLSQLKKEDITKKVKTVKVLKDKLENFRKIIG"
     gene            1563..>1777
                     /locus_tag="NBK22_002596"
     CDS             1563..>1777
                     /locus_tag="NBK22_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive phage resistance protein"
                     /protein_id="HCD2415748.1"
                     /translation="MKKSTLIDKFLDLLSSRSSLREIQNNFIDADIMRDSSINQKYNG
                     QRKSLAWEYISTLNLEDEAEFSKLLNV"
BASE COUNT          727 a          192 c          281 g          577 t
ORIGIN      
        1 ggttagataa aacaccacat ttctctggtt agtaaaaaca taacttctaa gacttttttt
       61 catatactaa taaattatat tataaatatt tttgaacata aaaggagtgt ataaaaatgt
      121 atgcaaagaa ggtgttttag tatagatttt aaggcattca tttttaatat aaaatattta
      181 taatatttaa aattgaaaat gattatcctt tcaaaatttt tgcaacgagt aattttactt
      241 taattatatt ataaagtaga attaatagta attaaaggaa cgtttgttcc tgtttttata
      301 tgtaaatacg agaacgattg aatagataaa gaaggataaa attgacttcg aaatagaata
      361 tttaatttca agaaggagag tattggatgt taaagcataa aataataaat ttaatacaag
      421 aaaagagaga agggagttac tgggatttta aagcagaata tcataaagat aaagcagaat
      481 tattacatga tataatttgt ttatcaaata acttgttgaa tcaagaagct tatttaattt
      541 taggagtcgc agataacggt catattctgg gggttgcagg tgattcaaat cgaaaaaacc
      601 aagaagaatt aatatcattt attactggaa aaaaatttgc tgcgggtagg caccccaaga
      661 tttcattaat gacatttgaa tatgaagaaa aggaaatcga tgtcattata ataaatccta
      721 aaggatatgt accatactat ctggagaaag cagaaaccga tcaaaaaagt aaaaaaaaca
      781 aaacagtcaa tgcaggtagc atttatacta gagttgaaga taaaaatacc ccaatagatt
      841 ctactgcaag tccattagat acagaaatat tatggaaaat gcattttggt ttatatccta
      901 ctcctataaa gaggttacag aattatttac taactcctga gaaatggatg cagaactcaa
      961 caggttattt ttatagtgaa tcgccagagt atatagtata caaaaatgag gacattgaag
     1021 aaaaagaaaa ttattttaat ttagtaagtc cattctatgc gtataatcaa atcaatagta
     1081 acactttata ctcatattac gaatttaaat atcatagtac agttttatat gcttgccggt
     1141 gtatctcttt agattcaggt atctacacaa caccagtacc taaatcaggt gagataaatt
     1201 ttaatatgca tagagatgat actatttatt atcgttattt cattgaagag acgatgcttt
     1261 ataatattca tctttttatg tataagggtg attcgatgga agagaaattt gcaatggaaa
     1321 aatttttaga gtgtgtttta gtttataaga acgaagagga aaaggaactt tttgaaaatt
     1381 atatattaca taattgggat aaagttaatc aatcaattaa tgaaaataat aaacgcgttt
     1441 ttggaactga acatttatca caacttaaaa aagaagatat cactaaaaaa gtaaaaacag
     1501 ttaaagtttt aaaagataaa cttgagaact ttagaaaaat tattggttaa taggagaaaa
     1561 tcatgaaaaa atcaacactc attgataaat ttttagactt actttcttca agaagctcac
     1621 tcagagaaat tcagaataat tttattgatg cagacattat gagagatagt tcgataaatc
     1681 aaaaatataa cggacaacgt aaatcgttag cgtgggaata tattagtact ttaaatttag
     1741 aagatgaagc tgaattttct aaattactta atgtttt
//
LOCUS       DAHHYJ010000230         1761 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000230 DAHHYJ010000000
VERSION     DAHHYJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.236"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            57..191
                     /locus_tag="NBK22_002597"
     CDS             57..191
                     /locus_tag="NBK22_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCD2415749.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            complement(788..1573)
                     /locus_tag="NBK22_002598"
     CDS             complement(788..1573)
                     /locus_tag="NBK22_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCD2415750.1"
                     /translation="MKVQIYQLPIIFGDSSKNEAQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSDGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
BASE COUNT          612 a          283 c          265 g          601 t
ORIGIN      
        1 tgcttttagc atgttttaat ataactagat cacagagatg tgatggaaaa tagttgatga
       61 gttgtttaat tttaagaatt tttatcttaa ttaaggaagg agtgatttca atggcacaag
      121 atatcatttc aacaatcggt gacttagtaa aatggattat cgacacagtg aacaaattca
      181 ctaaaaaata agatgaataa ttaattaatt tcattgtaaa tttgttatct tcgtatagta
      241 ctaaaagtat gagttattaa gccatcccaa cttaataacc atgtaaaatt agcaagtgag
      301 taacatttgc tagtagagtt agtttccttg gactcagtgc tatgtatttt tcttaattat
      361 cattacagat aattatttct agcatgtaag ctatcgtaaa caacatcgat ttatcattat
      421 ttgataaata aaattttttt cataattaat aacatcccca aaaatagatt gaaaaaataa
      481 ctgtaaaaca ttcccttaat aataagtatg gtcgtgagcc cctcccaagc tcgcggcctt
      541 ttttgtaatg aagaagggat gagttaatca tcattatgag acccgccgtt aaaatttttg
      601 aataagtcta atgttggaaa aggtcaaaag aataatcaat ttaattaaga aaatcattca
      661 tttgcaaagg gcgaaatggg ttcttactga gttatctatt ataaaaaaat aaacatagac
      721 ttatgaaaaa tctctcataa atctatgttt agtcatgaca tgtgttaaat attatttcgg
      781 gcgcttctta tttatacaaa tctaatttaa tacttttaaa tacaggtata ttttcgcgtt
      841 gctgttctac ttcatttaag tttaaatcta cagtcaaaat atctgcggat tcatttaatt
      901 ctccaactaa atctccattt gggtttataa ctatcgaatg accagcatat tctgtgttac
      961 catcgaatcc agtgctatta gttccaatga caaacatatt attttcaatt gcacgtgcct
     1021 ttagtaatga atgccaatgt tgaagacgtg acataggcca ttgcgccaca taaaatgcaa
     1081 ttttagcacc actgcgagca ggatatctta ataattctgg aaaacgtaaa tcataacaga
     1141 taagttgggt cacataagta ccgtcagaca attgaaaggg ttcagctacg ttttcgccag
     1201 cagttaaaaa ttcatgctct cttaacatag gaactaaatg aactttgtcg tattcattaa
     1261 ttagctggcc acttttattc acactaaaag ctgtattaaa tatttgattg tttctaatgt
     1321 tagaaactga cccagctacg atatcgactt tatatttttc agctaaatgt ttaataaatg
     1381 aaaaactttg tcctagatta ttatctgctt tttcatttaa atgctctaaa tcatagccat
     1441 tattccacat ttcaggtaaa acgactacat ctacttcagc attcatattt ttttcgaacc
     1501 attgcgttat ttgagcttca tttttagaac tatctccaaa aataatcggt aattgataaa
     1561 tttggacttt cataacatca catccttgat agatcttata tataacttac taaaagttat
     1621 gttgaaacgc aaaaaacgag cccaggacat aaaatcaaag tcctaggctc tacaatgtta
     1681 tattgacagt agttgatggg gccccaacat agagaaattg gaacaccaat ttctacagac
     1741 aatgcaagtt ggggtgggct c
//
LOCUS       DAHHYJ010000231         1749 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.237, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000231 DAHHYJ010000000
VERSION     DAHHYJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.237"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..536
                     /locus_tag="NBK22_002599"
     CDS             <1..536
                     /locus_tag="NBK22_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415751.1"
                     /translation="ILLMIMIIPTWINLLLKTYAFIGLLSHDGVINQFFHLFNLPSFN
                     LLFTTGAFLVVASYIYIPFMILPIFNSMKAIPNNLLQASSDLGASPFYTFRKVIMPLT
                     KEGVMTGIQVTFIPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMGSTIAIFLI
                     VFMAFILIITKSSNGRG"
     gene            542..1351
                     /locus_tag="NBK22_002600"
     CDS             542..1351
                     /locus_tag="NBK22_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415752.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            1351..>1749
                     /locus_tag="NBK22_002601"
     CDS             1351..>1749
                     /locus_tag="NBK22_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD2415753.1"
                     /translation="MKRFLQLIIGALVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDRGNKYS"
BASE COUNT          587 a          228 c          282 g          652 t
ORIGIN      
        1 atatcttatt aatgataatg attattccaa catggataaa tttattgtta aagacatatg
       61 cttttatagg tttattaagt catgatggcg tgattaatca atttttccac ttatttaatt
      121 taccatcatt taatctgtta tttacaactg gtgcgttttt agtagtagca agttatattt
      181 atataccatt tatgatttta cctatattta atagcatgaa agcaattcct aataatttat
      241 tgcaggcctc aagtgattta ggtgctagtc ctttctatac ttttagaaaa gtaatcatgc
      301 cgttaacaaa agaaggtgtt atgactggaa ttcaagtaac ctttattcca tcactttcat
      361 tatttatgat tacaagatta attgcaggta ataaagtcat aaatataggt acggcaatag
      421 aggaacaatt tttaacaatt caaaattatg gtatgggatc aactatagct atattcttaa
      481 ttgtatttat ggcattcatt ttaatcatta caaaatcatc taatgggaga gggtgaatca
      541 tatgaaatgg tatggaaagc tgtatatcgg gatactttta gcgattttat acatcccaat
      601 attcttttta atgttctatt cattcaattc ggctggtaat atgattcact ttgaacattt
      661 tacattagag cattatcaat cattatttca aaatgatcat ttaatgtcgg tcatttttaa
      721 tacgatagct gtagcacttt tagcagcctc aatttctaca gttattggta catttggtgc
      781 cattgctatt tattatttaa gaaataaaaa gtttaaagta actttactaa cattgaataa
      841 tgtcttgatg gtatcatccg acgttgtcat aggtgcatca ttcttaatta tgtttacaac
      901 gattggccat tttactggtc ttggtttagg attttggaca gttctaatat ctcatattgc
      961 attttgcata cctatagttg tgattatcgt cttaccacaa ctgtatgaaa tgaataataa
     1021 tatgttaaat gctgcaagag atttaggagc gactgaacca caattattaa gcaacattat
     1081 tattcctaat attttacctt ctattatagg aggattcttt atggctttaa cttattcact
     1141 agacgatttc acagtaagtt tcttcgttac tggtaatggc tttagtgtgt tatcagttga
     1201 agtttatgct atggcgagaa aaggaattag tatggaaatt aatgcgattt caacattatt
     1261 gtttgctgtt attgtattag gaatactagg atattatttg attcaatacg tgataaataa
     1321 gaaaaaacta atcaagcgag gtgtaaaata atgaaacgat ttttacaact cattataggt
     1381 gcattagttg tgggtatgct ttgtcttact ttaagtcatt ggtttaaatc taaagaacaa
     1441 gtgcatacaa atcaaaaaat ttacgtatac aattggggcg aatatattga tccagagtta
     1501 attaagaaat ttgaaaaaga aactggcatt caagtcgttt atgaaacttt cgattcaaat
     1561 gaagcgatgg aagccaaaat tcgcaatggc ggtacacatt atgatgttgc ttttcctagt
     1621 gaatatacag ttcaaaaatt gaaaagagat catttattgt taccaataga tcataataag
     1681 gtacctaata ttaaaaattt agattcagat tatatgaata tgtcatttga tagaggcaat
     1741 aaatattct
//
LOCUS       DAHHYJ010000232         1731 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000232 DAHHYJ010000000
VERSION     DAHHYJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.238"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..182
                     /locus_tag="NBK22_002602"
     CDS             <1..182
                     /locus_tag="NBK22_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HCD2415754.1"
                     /translation="MLHNQLRPLNYEEDIKKGLEDIERFAKENQLQRISPYYFILNDV
                     NGFKWIDIKVKVMEY"
     gene            192..803
                     /locus_tag="NBK22_002603"
     CDS             192..803
                     /locus_tag="NBK22_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
                     /protein_id="HCD2415755.1"
                     /translation="MEFLLLIVVAGLYYIIYLTAVMYSEKIVVLPIIIYAIVFVIIGI
                     TYIFIGDSYDQLTNFNVILYMGSLFYAWMAIRNLWNRPLLLKYKNITDSSSGIVNKSE
                     YNSVESLRINIEIAKYKGIISLIVAIVLTVLMILKSTPQITAETRDLSISFFILSLFI
                     IIIFAVWDLFIRVRKGAFAFVVIRPILFSCWLFILNMILSRLL"
     gene            939..1157
                     /locus_tag="NBK22_002604"
     CDS             939..1157
                     /locus_tag="NBK22_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415756.1"
                     /translation="MENQKQGNGLKIATWVFIVLTVVTPLFGIGSIVCSINYKKYDAE
                     KGSKLLQIAIIVTIIAFVLNLLAYLGLR"
     gene            1289..>1731
                     /locus_tag="NBK22_002605"
     CDS             1289..>1731
                     /locus_tag="NBK22_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD2415757.1"
                     /translation="MTFEEKLSEIYDKIANEISGMIPVEWEQVFTIAYVDDEGGEVVF
                     NYTKPNSDDLNYYTYIPREYNISEKVFYDLWTDLYILFKKLRNAFKEEDLEPWTSCEF
                     DFTRDGKLNVSFDYIDWVILGFGPLAKENYYMYKKFGVMPEMKYE"
BASE COUNT          646 a          163 c          310 g          612 t
ORIGIN      
        1 aaatgttaca taatcaactt agaccactaa attatgaaga agatattaag aagggtttag
       61 aagatataga aagatttgca aaagaaaatc aacttcaacg tatatcacca tattatttta
      121 tactcaatga tgtgaatgga tttaaatgga tcgatattaa agtaaaagtt atggaatatt
      181 gaggtgcgaa tatggagttc ttattattaa ttgtcgtagc cggactgtat tatattatat
      241 atttaactgc tgtgatgtat tctgaaaaaa tagtagtatt gcctataatc atctatgcca
      301 ttgtgtttgt aataattggt atcacttata tctttatagg cgacagctat gatcaattaa
      361 caaatttcaa tgtgattttg tatatgggga gtttgtttta tgcatggatg gctattagaa
      421 atctttggaa cagaccacta ttactaaaat ataagaacat tacagatagt tcaagtggaa
      481 tagttaataa atctgaatac aattcagttg aaagcttacg tataaatatt gaaatagcta
      541 agtataaagg gattatttct ttgatagtag ctatagtact aacggtatta atgatattaa
      601 agtcaacacc tcaaatcact gcagaaacac gtgacttaag tatctcattt ttcatactca
      661 gcttatttat cattattata tttgcagttt gggatttgtt tattagagtt agaaaaggag
      721 cgtttgcttt tgttgtaata aggccaatat tatttagttg ttggttattt attttgaata
      781 tgattttatc aagattatta taattttatt acatataggt gtgggtaaat ttttatttat
      841 gaaatgatga aaaagtatat actattaata tcttttttac tataattata aatagttaaa
      901 taaattgaaa gacaaaataa tgagtaggag ttaatgagat ggaaaaccaa aaacaaggca
      961 atggcttaaa aattgcaaca tgggtattta ttgtattaac agtagttaca ccgctatttg
     1021 gtattggaag tattgtttgt agtattaatt ataaaaaata cgatgctgaa aaaggttcga
     1081 agttattgca aattgcaatt atcgtaacaa taattgcttt tgttttaaat ttattagcat
     1141 atttaggttt aagataagta aaatgaattt gaaaaagcat taagcgacat ttgggtgttg
     1201 tttaatgctt tttatttaat agtaataagt gtagagaata aattaatagt ttaatcaaga
     1261 gatatttcga acacataggg gcgataacat gacttttgaa gaaaaactaa gtgaaatata
     1321 cgacaagatt gcgaatgaga ttagtgggat gataccagta gaatgggagc aagtatttac
     1381 aatagcctac gtagatgatg aaggtggaga ggtcgttttt aattatacta aaccaaacag
     1441 cgatgacttg aattattaca catatatccc tagagagtat aatatctctg aaaaagtatt
     1501 ttatgatttg tggacggatt tatatatatt gtttaagaag ttaagaaacg catttaaaga
     1561 agaagattta gaaccatgga catcatgcga atttgatttt acaagagatg gtaaattgaa
     1621 tgtatcattt gattatatag attgggttat attaggtttt ggaccattag caaaagaaaa
     1681 ttattatatg tataaaaaat ttggagttat gccagaaatg aaatatgaaa t
//
LOCUS       DAHHYJ010000233         1715 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000233 DAHHYJ010000000
VERSION     DAHHYJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.239"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(75..935)
                     /locus_tag="NBK22_002606"
     CDS             complement(75..935)
                     /locus_tag="NBK22_002606"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCD2415758.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            1153..1674
                     /locus_tag="NBK22_002607"
     CDS             1153..1674
                     /locus_tag="NBK22_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCD2415759.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHSDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
BASE COUNT          551 a          304 c          263 g          597 t
ORIGIN      
        1 gcaaacacca ttttgtctgc ttcttttaaa attaatatcc ctacgttatt aataacttaa
       61 aaactaaata ttaattattt agcgcggttt gaagtaccga actctttaat tttaccttta
      121 actgtttctt tgatagcttc acgtgcaggt cctaagtatt tacgaggatc gtaaacttct
      181 ttgtcgttat ttaaaacgtc acgaactgct tttgctgaag cgatttggtt ttcagtgttt
      241 acgttaattt tagctgtacc aaatggaatt gctttttgga tatctttagt cgggatacca
      301 gtaccaccgt gtaatactaa tggcaaacct gtagataaac cgatttcttc catttcttta
      361 aatcctaatt ttggttcacc tttgtatgga ccatgaactg aacctaatgc tggcgctaat
      421 gcatcaatac cagttttttc aactagttct tgacattctt taggatcagc ataaatgatg
      481 ccgtctgcta caacatcatc ttcttgtcca ccaacagtac ctaattcagc ttctacagaa
      541 acaccttttt catgagcgta ttcaacaact tttttagttg ttgctacgtt ttcttcgaat
      601 gggctgtgtg aagcatcgat cattactgat gtgaaaccag catcgatagc ttctttacat
      661 ttttcaaagc ttgaaccatg gtctaaatgg attgctacag gaatagtgat gtttaagtca
      721 tgcattaacc cttcaaccat tttaacaatt gtgtagaaac cgctcatgta acgagcagca
      781 ccttcagaaa cacctaaaat tacaggtgca ttttcttctt gtgacgcttc taaaattgct
      841 tgagtgaatt ctaggttatt aatattgtat tgacctaccg cataaccatt ttcttttgca
      901 tcaattaaca tttctttcat tgaaactaaa ggcatgaaag ttcctccttg agtgcttacg
      961 cacgtatttt tcaaagtaat tatatcaaaa aaatagcgtc aatgcattga aatactgcag
     1021 attaaatgtg tattttttga aaaatgcgat agttatacta aatgcaaacg tatacatttt
     1081 tatacaattt aagccgttgc ataatatgta gtcattaatt acaatatttt ataagtagat
     1141 tggaggataa agatgtcaaa aatcttaaac acacaactaa taggtatctt taataggcta
     1201 gaaaaacaat ctttagaaat tcaaatggcc gcccaatgtt taattcaagc aattggtggc
     1261 gaaggttatg tttatgtaaa aggttatgat gatttacagt ttttcgaaag tttcattcta
     1321 catagtgatg agcgtttaaa atctagccga aaacttgacg ctattaaaga ctttaaagaa
     1381 attgactcaa ctgatcgtgt attattattc gcaccatttt ataatgatca agttgcatta
     1441 gatattcaaa aacttatcga cttagatatc gatgtggtct taataagcaa caaacctaaa
     1501 acagatgatt tccctgatca tctcgtacat tttatcgatt tatctacacc aagacctata
     1561 gtctacactg aagattacga taagattgtc caaccacatg cgatggcatt gaattatgta
     1621 tactatgaca tttatacaca aatgattgaa atgacaagag acttagaatt ataaaagtca
     1681 ctattgttat ttcatacctt gtcactaaca taata
//
LOCUS       DAHHYJ010000234         1709 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.240, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000234 DAHHYJ010000000
VERSION     DAHHYJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.240"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..1174)
                     /locus_tag="NBK22_002608"
     CDS             complement(<1..1174)
                     /locus_tag="NBK22_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD2415760.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDEPGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEY"
     gene            1335..>1709
                     /locus_tag="NBK22_002609"
     CDS             1335..>1709
                     /locus_tag="NBK22_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD2415761.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAP"
BASE COUNT          664 a          223 c          254 g          568 t
ORIGIN      
        1 gatattctgt ggtaaggttc atacttaacc ctactgttcc gaatgcagaa accacttcga
       61 ataataactt gattaatgat atgttcggat taattatcga taatataaaa gtaatgatac
      121 tgataaatag aaatgagata ttaatggtaa caatagatag ttttatatgt ttgtcagata
      181 tttctttatt gaatactgaa acattatttt ctttacgtat ataatttagt acaaaaataa
      241 acgctactgc aaaagtagtt attttaattc ctccagctgc actgagaggg gcaccaccaa
      301 taaacataag tagcattaac attaaggcgg tagatttgtt aatgcttgct atatctatac
      361 tgttaaaacc cgctgttcgt gttgttactg attggaaaaa agaatttccg attttttcaa
      421 ctagtcccat atgttgcata gtattaaact gttctaataa aaagaatgta atagctccta
      481 taattattag gatactagtt gtagttaaga ctaatttaga atgtaaagat aatttactca
      541 attttttaca attaataaag tctatcacga caaaatgtcc aatacctcca aatattatga
      601 gtattgagat tgtaataatg acaattggat cactagaata atctattaag ttattcttaa
      661 aaagggcaaa tccagcatta ttaaaagctg atactgatgt gaataagctt aaaaataaac
      721 ctttgcctat accaaatttt ggtataaaag ataaacacaa acaaatcata ccaattaatt
      781 cagtgactaa actataaata gccaagtgtt taattagttt aataacacca ccaggttcgt
      841 caatattcca tgtaaccata atcaagaatc tatttttcat tgatatcttt ctatttaaaa
      901 atactagtgt caatagggtt acggtcacga tacccagacc acctatttga attaataata
      961 gtattactat ttcaccaagt atattaaact gtgatcctat atcaactggg gataggccag
     1021 taactgtaaa tgcacttgaa gctataaata gggcatctaa aaaagatatt ggctttttac
     1081 cagtgaaagg taaatacaat aaaagtgcac ctatgatagt tgtagagaag aaaagcatta
     1141 aataaaaata taaaggtttg tggactttgt tcattttaat ttcatactcc tttatattat
     1201 aaattaataa ttagataata tcataaatga aaatagaaat gttactgatg tgtattacta
     1261 acaggcgttg actaattctt ttaatatttt ataattgaaa atgaatatca attaaaaaac
     1321 aaaaggatga tattatgaaa agattaattg gaattttatt atgtaattta tttattttga
     1381 ctgcgtgttc agcatctgtc gataaaacaa gtaactcgac aaagactaca gactataaaa
     1441 ttgaaaatgg tgaaacactg aaagtaccag agaaacctaa aagagttgct gtattaactg
     1501 gattttatgt tggtgatttt ataaagttag gaatcaaacc aattgctgtt tcagatataa
     1561 ctaaagattc ttcaatttta aaaccctatt taaaaggggt tgattatatt ggagaaaatg
     1621 atgttgaaag agttgctaaa gcaaaaccag atttaattgt tgtagatgct atggataaaa
     1681 atataaaaaa ataccaaaaa atagcgcca
//
LOCUS       DAHHYJ010000235         1661 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000235 DAHHYJ010000000
VERSION     DAHHYJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.241"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(62..580)
                     /locus_tag="NBK22_002610"
     CDS             complement(62..580)
                     /locus_tag="NBK22_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCD2415762.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYTKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDDIALQFNTKKHQILQHSLTIRQYEMQK
                     QHYFEKLCKKYL"
     gene            700..1578
                     /locus_tag="NBK22_002611"
     CDS             700..1578
                     /locus_tag="NBK22_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCD2415763.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
BASE COUNT          683 a          187 c          261 g          530 t
ORIGIN      
        1 tgcgaaaatt gaaaagaaag tattagaatt agtataatta taaaatacgg cattattgtc
       61 gttataagta ttttttacat agtttttcaa agtaatgttg cttttgcatc tcatattgtc
      121 taattgttaa gctatgttgc aatatttggt gcttttttgt attgaattgc aaagcaatat
      181 catcattagt tgataagagg taatcaagtg caagataaga ttcaaatgtt tgggtattca
      241 tttgaatgat atgtagacgc acctgttgtt ttagttcatg aaaattgtta aacttcgcca
      301 tcataacttt cttagtatat ttatgatgca aacgataaaa ccctacataa tttaagcgtt
      361 tttcatctaa ggatgtaata tcatgcaaat tttctacacc tactaaaata tctaaaattg
      421 gctctgttga atatttaaaa tgatgcgtac cgccaatatg ttttgtatat tttactgggc
      481 tgtctaagag gttgaataat aatgattcaa tttcagtgta ttgtgattga aaacaattag
      541 ttaaatcact attaatgaat ggttgaacat ttgaatacat gataaactcc tttgatattg
      601 aaatttaatt taatcacgat aaagtctgga atactataac ataattcatt ttcataataa
      661 acatgttttt gtataatgaa tctgttaagg agtgcaatca tgaaaaaaat tgttattatc
      721 gctgttttag cgattttatt tgtagtaata agtgcttgtg gtaataaaga aaaagaggca
      781 caacatcaat ttactaagca atttaaagat gttgagcaaa aacaaaaaga attacaacat
      841 gtcatggata atatacattt gaaagaaatt gatcatctaa gtaaaactga tacaactgat
      901 aaaaatagta aagaatttaa ggcactacaa gaagatgtta aaaaccatct catacctaaa
      961 tttgaagcat attataagtc agcaaaaaat ttgcctgatg atacaatgaa agttaagaaa
     1021 ttaaaaaaag aatatatgac gcttgcaaat gagaagaagg atgcgatata tcaattaaaa
     1081 aaattcatag gtttatgtaa tcaatctatc aagtataacg aagacatttt agattatacg
     1141 aaacaatttg aaaaaaatag atacaaagtt gaatcagaaa ttaaattagc tgataataaa
     1201 agtgaagcaa ctaatcttac gacaaaatta gaacataata ataaagcgtt aagagatact
     1261 gcgaagaaga acctagatga tagtaaagaa aatgaagtaa aaggcgcgat taaaaatcac
     1321 attatgccaa tgattgaaaa acaaattacc gatattaacc aaactaatat tagtgataag
     1381 catgttaata atgcaaggaa aaacgcaata gaaatgtatt acagtctgca gaactattat
     1441 aatacacgta ttgaaacaat aaaggttagt gagaagttat caaaagtcga tgtagataag
     1501 ttgccgaaaa agggtataga tataactcac ggcgataaag cctttgaaaa aaagcttgaa
     1561 aaattagaag aaaaataact ataatcattt ttcaaagtta aaaattttga atttatggtt
     1621 aacatgtcaa cttactatgt gtataatggt aaacattgat a
//
LOCUS       DAHHYJ010000236         1645 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000236 DAHHYJ010000000
VERSION     DAHHYJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.242"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            84..701
                     /locus_tag="NBK22_002612"
     CDS             84..701
                     /locus_tag="NBK22_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD2415764.1"
                     /translation="MLCETEIINKNPKYRVIKYDDEYLMVDVISTWLVYFFPFINWFI
                     PKRCAKISREEYEKLNTVKPVKNKNFWPVVGGTILLGATSRKYIHLLNIQLEKRSVIF
                     ICFVVFLCILIFFVLLNRKLKLKVFDNKKEEQKIILVPTLKNAVLILYGYLLIGGMSI
                     LALSMLLTLENQNLITFIAWGMGLMLFFLMNITLIVNKTVKVIKR"
     gene            776..1405
                     /locus_tag="NBK22_002613"
     CDS             776..1405
                     /locus_tag="NBK22_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 domain-containing protein"
                     /protein_id="HCD2415765.1"
                     /translation="MLCESRVINQNPKYRIIKYNNEYFMVDLVSTWIAYFLPMINWFI
                     PKKYAKISREEFESLNIVKPAKNNTFWPVAGFAVLLTTLTRKYIYLLNIHLEKEIVIL
                     TCCMILLGVFALFIYINTKLKLHIFDKNKSNNEKIILIPTFKNICLSLFAYILFGGLS
                     TMALSMLVTSSPQNIIEFLALIGMTACFFLLNMSSVLDKNIHVILKTNK"
     gene            1462..1578
                     /locus_tag="NBK22_002614"
     CDS             1462..1578
                     /locus_tag="NBK22_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415766.1"
                     /translation="MILNRGAILEQGNKTAKVIKSELITNYIMLATLQTKNN"
BASE COUNT          628 a          193 c          256 g          568 t
ORIGIN      
        1 gatacaaaag ggcacaagtg ttttgagaaa cagaaacgat aatatagctg tgtgaatttg
       61 ttattaatag gaagtgataa ttattgcttt gtgaaaccga aattatcaat aaaaatccga
      121 aatacagagt tattaagtat gatgatgagt atttaatggt cgatgtaata agcacttggc
      181 ttgtatactt ttttccattt attaattggt tcattccaaa aaggtgcgcc aaaattagta
      241 gagaagagta tgaaaaactg aacactgtta agccagttaa aaataaaaat ttttggcctg
      301 ttgtaggagg aacaatccta ctaggtgcta cttccagaaa gtacatacac ttacttaata
      361 ttcaattaga aaaaagatcg gttatattta tatgcttcgt tgtatttcta tgtattttaa
      421 tcttttttgt gcttctgaat cgaaaattaa aattgaaagt tttcgataat aaaaaagaag
      481 aacaaaaaat aattttagta ccaacattaa aaaatgctgt tcttatatta tatggctatt
      541 tgttgattgg aggcatgtca atattagctt taagcatgtt gctgacatta gaaaaccaga
      601 atttaataac ttttatagct tggggtatgg ggttaatgtt gtttttcttg atgaatataa
      661 cactcattgt caataaaaca gtcaaagtta taaaaaggtg aataaataac taattatatt
      721 gtattggccg ttcttcaaat aaaaaataat ttgaataagg tggaagtgat aaacagtgct
      781 ttgcgaatct agagtcatta atcaaaaccc taaatataga attattaaat acaataatga
      841 atatttcatg gttgatttag taagtacttg gattgcttat tttttgccca tgattaattg
      901 gtttattccc aaaaagtacg cgaaaattag tagagaagaa tttgaaagtt taaatattgt
      961 caaacccgct aaaaataata ctttctggcc tgttgcagga tttgcagtgt tattaacaac
     1021 cttaacaaga aaatatatct atttgcttaa catccattta gaaaaagaaa tagttatatt
     1081 aacatgctgt atgatacttc taggtgtttt cgcattgttt atatatataa atacaaaatt
     1141 gaagttacat atttttgata aaaataaaag taataacgaa aagatcatat taatacctac
     1201 atttaaaaat atttgtttat ccttatttgc ttatatatta tttggtggat tgtcaacaat
     1261 ggctctgagt atgttagtaa cttcatcccc tcaaaatata atagaatttc ttgctttaat
     1321 tggcatgact gcatgcttct ttctactgaa tatgtcatcg gttctagata aaaatattca
     1381 tgttatttta aaaacaaata agtagtaaaa ttgattaact taggtagtat cggatactta
     1441 aatgttggtt cataaaaagc aatgatttta aatcgaggag ctatcttaga acagggaaat
     1501 aaaacagcta aagttataaa aagcgaatta ataactaatt atattatgtt agccactctt
     1561 caaacaaaaa ataattagaa taaggtggaa ttgataatca atgctatgcg aatctaaaat
     1621 catcaataaa aatccaaaat ataga
//
LOCUS       DAHHYJ010000237         1540 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000237 DAHHYJ010000000
VERSION     DAHHYJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.243"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(52..1525)
                     /gene="cls"
                     /locus_tag="NBK22_002615"
                     /pseudo
     CDS             complement(52..1525)
                     /gene="cls"
                     /locus_tag="NBK22_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
BASE COUNT          521 a          296 c          201 g          522 t
ORIGIN      
        1 ttttgggcaa aataaaaaga gcctctataa tcgagactcc ttacaaataa attataaaat
       61 tggcgaaact aatttagcta acgattcttt aaacttaaca gacagcggtc tattggcata
      121 tgattctttg gttagttgtt ttgattttgt aatatcatgt tcataagcca cccttaaatc
      181 tttagcaagc ttttcatcat atacaaaagc attgacttcg aaattcaatt caaaacttct
      241 aaagtccata ttagcagttc cgactgaaac catttcatca tctattaaac acattttaga
      301 atgtataaat ccattttcat acgtgtaaat ttttacgcca ctagatagta aatctgaagc
      361 atttgaatat gtcgcccaat aaactaaagg atgatctggc ttacatggaa tcattagatg
      421 aacatctacc cctgatttag ctgcaatttt aatggcattt atatatgagt tatccggaat
      481 gaaataaggc gattgcaaat ataccgactt cttagcgctc ataatcattt ttgtataacc
      541 atattcaatt tgatgccagt cactagccgg accacttgca gctatttgaa ttgcccaatg
      601 gtccgttctt tttagggaaa tacttaacat catattcaaa ctgtggacgg tgcgcttgcg
      661 aattccagtc taaaataaat cgcaactgca gtgcatcaac cgcatcccct tgtatacgta
      721 aatgcgtatc tctccaatac cctagtttac ctaatcctag atattcatca ccaatgttaa
      781 atcctccgac ataacctagt tgaccatcga ttacgatgat ttttctatga tttctgttat
      841 tcattctgaa attcaataac ggtaatttag aagcaaaaaa tgcttcaact tctccaccta
      901 acgatttaaa atgatcaaaa ttagccatct taactttctt tgaaccaacg tcatcatata
      961 aaattttcac ttctagacct tgtttcaatt tctcttctaa cgcttttaaa attctttggc
     1021 ccaagccatc tagagcgaat gtataatact ctaaatggat gtattcttta gcatttttaa
     1081 tatcttgtaa aacttgatca tataaatcat ttccatcaat gaaatgatca actttattat
     1141 tttcagttaa aaaaccatct tgatccatca aaagcatacg tactaaatca tgatgttttt
     1201 gaacttgttt gttatcagta ccataattac ctttatcaaa gctttctatt tgttgtttta
     1261 aaagtccatc gaaatccgtt aacacgttac cattgttttt attcaattta cgtgccgaaa
     1321 cggttctacc aaaaaacaag taaagaataa aaccaatcaa tggtaagaca aaaagtacaa
     1381 atagccatgc ccaagttgaa ctcgctgtac gtctatttct ttctaaaaag ataataataa
     1441 aagccaatac taaattaatg atgaatccaa tggctaaaat aatggtaaaa agtgttccta
     1501 aatcatttga aaatgtatat cgcatagtct tttctcctat
//
LOCUS       DAHHYJ010000238         1536 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000238 DAHHYJ010000000
VERSION     DAHHYJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.244"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(30..527)
                     /gene="efb"
                     /locus_tag="NBK22_002616"
     CDS             complement(30..527)
                     /gene="efb"
                     /locus_tag="NBK22_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Efb"
                     /protein_id="HCD2415767.1"
                     /translation="MKNKLIAKSLLTIAAIGITTTTIASTADASEGYGPREKKPVSIN
                     HNIVEYNDGTFKYQSRPKFNSTPKYIKFKHDYNILEFNDGTFEYGARPQFNKPAAKTD
                     ATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQKAVNLVSFEYKVKKMVLQERIDNVLK
                     QGLVR"
     gene            complement(787..1293)
                     /locus_tag="NBK22_002617"
     CDS             complement(787..1293)
                     /locus_tag="NBK22_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415768.1"
                     /translation="MIIKILTILLLLCILSYLVTNRKKPFLFLKTLFMGVVFIFIGYI
                     SLAISAVIIYGIIQFITIDFGSFFLMGIILILISSIFQLFIVRLLFRKKNVDLTEVVV
                     LEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVFFDVSNLNLVILPTLIISWVTIF
                     NYRMRSYK"
BASE COUNT          538 a          241 c          183 g          574 t
ORIGIN      
        1 attttgaact taaaataatg acagaagtat tatctcacta atccttgttt taatacatta
       61 tcaattcgct cttgtaagac cattttctta actttgtatt caaacgaaac taagttgact
      121 gccttttgtg cttttctgtg tgcactgaca gtatgtgttt tttcaaattc tctcacaaga
      181 ttttgagctt gaatcaattt ttgttctttt ttaatagttg catcagtttt cgctgctggt
      241 ttattaaatt gtggacgcgc accatattcg aatgtaccat cgttaaattc taaaatatta
      301 tagtcatgtt tgaatttaat atatttaggt gttgagttaa atttcggtct agattgatat
      361 ttaaaagtac catcattgta ctctacgata ttgtgattaa tactcactgg tttcttttct
      421 cttggaccgt atccttcgct cgcatctgct gttgacgcaa ttgtagttgt agtaatacct
      481 attgccgcta ttgttaataa agattttgct atcaatttat ttttcatgtt aattatcctc
      541 caaattatta tcttacattt attaagttat cctatttata caacttttga aattaactaa
      601 ttattaatta actataaaca ctttataata tagacaaaat atagttatct atcttgtgac
      661 tcatcagcac tgatacacta gtcaaaaaat tcacgcctct actttttcga atttcaaaaa
      721 taaaaccaca caacaaaaaa agacaccatt tgaatgttgg tgcctgatgg tataatttca
      781 tagattttat ttgtaacttc tcattctata gttaaatatt gtgacccagc taatgattaa
      841 cgttggtaga attactaaat tcaaattaga cacgtcaaaa aagacagatt ggaaattgtc
      901 gatattaata tcattaatgt ccattttttc atttactgct tgatagatcg caaagtaaac
      961 taagaaccat tgaattaaat gctctaaaac gacaacctct gtcaaatcga cattcttttt
     1021 tctaaaaagt aatctaacta taaataattg gaatatactt gaaatcaaga tcaatataat
     1081 acccattaag aaaaaactac caaaatcaat tgtgataaat tgaataatgc cataaataat
     1141 tacggcagat attgccagtg aaatatatcc tataaagata aataccacac ccataaagag
     1201 tgtttttaag aacagaaaag gcttctttct atttgtaacc aaatagctca atatacaaag
     1261 tagtaataga attgttaaaa ttttaattat catcgtttga cctcaatata tttatttata
     1321 tctttattat tgaccacgaa aaaataacaa tcacttaagt gattgccaat aatactcaat
     1381 gcctactcta tagtaatctt ggcacgtgat ttcacactat tttatctatt taaaaatgta
     1441 agtgttgaat tatgcttact tcgaaaatgc ataattcaat taaatagtat aatttttaac
     1501 ttatatatta atcataacat atcaacctta attaga
//
LOCUS       DAHHYJ010000239         1533 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.245, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000239 DAHHYJ010000000
VERSION     DAHHYJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.245"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            63..365
                     /locus_tag="NBK22_002618"
     CDS             63..365
                     /locus_tag="NBK22_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415769.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGFFNSFIKLILFCSV
                     INSIVLILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVRTFGFI"
     gene            621..986
                     /gene="crcB"
                     /locus_tag="NBK22_002619"
     CDS             621..986
                     /gene="crcB"
                     /locus_tag="NBK22_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCD2415770.1"
                     /translation="MQYVYIFIGGALGTLLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAVTS
                     YVFGILLCYVGIKLGGGLS"
     gene            983..1336
                     /locus_tag="NBK22_002620"
     CDS             983..1336
                     /locus_tag="NBK22_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCD2415771.1"
                     /translation="MISIILVMIGGGFGAIARSAITDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMTPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT          485 a          241 c          219 g          588 t
ORIGIN      
        1 actattgttt tatgattgtt tatgatacga tatgtaaatg ataatcatcc aggaggtcta
       61 gcatgtctcg ttcaaaaaaa tacttttact tatctagctt aatgattatt ttaagctttt
      121 tctttaatac aaataacgtt ttcctaagtg gattttttaa ttcttttatt aaattaatac
      181 ttttctgcag tgttattaac tcaattgtac taattttgtc tataattttt gcagatcgtt
      241 caattaaatc actaaagcct gatgcagatt ggattagaat tgcgagtaaa agtttgcctt
      301 ggattattct aattgttatt ttagtacata tcttttcaat tgttcgtaca ttcggtttta
      361 tttaaaaaag ataaaatgtc aatgtagcct taatacaaaa caatacaatg tatcatgcta
      421 taatgagtaa aacaatttga taacattgtt gcgtataaaa aatattagat tttcgaaatc
      481 ataactatgc atctaatcgc tatagttata caacaagata taacatataa tgaggtttga
      541 taatgcatcg acaatttttg tcgtcgcgtt gccaaaacct cttttttaaa ttcaaactac
      601 ttcttttcga ggtgaaccaa atgcaatatg tatatatttt tatcggtggt gctttaggca
      661 ctttattacg ttacctcatt tcttttctga atactgacgg aggttttcca atcggaacac
      721 tgatagccaa tttgactggt gcctttgtaa tgggattgct aacagcctta acaattgcat
      781 ttttttcaaa ccatccgacg ctaaaaaaag ctattacgac tggttttctt ggtgctttaa
      841 cgactttttc aacatttcaa ttagaattaa tacatatgtt tgatcatcaa caatttataa
      901 ctttactact atatgctgta acaagttatg tctttggtat tttgttatgt tacgtcggta
      961 taaaactagg tggtggttta tcatgatatc aatcatttta gtcatgattg gcggcggttt
     1021 tggcgcaatt gctagaagtg ccattactga ttattttaat cataaattta cttcaaagtt
     1081 acctatcgca acattgatag taaatctagt tggtagtttt ttaattggat taactatagg
     1141 cctatcaatt tcaatctcat ggttccctgc gttctttgtt accggttttt taggtggctt
     1201 aacaactttc tcaacgttag ccaaagaact tacactaatg atgacgccaa aatttaatat
     1261 taaccttttt ctcaattatt cacttttaca attcatcatt ggatttatag cttgttatat
     1321 tggctatcat atttaaaaat aaaatgcttc attcagcaaa taggtaaatt acgacacctt
     1381 cctgaacgaa gcatttttta attttcatgc aaatttttta gcaccatata atgcctatca
     1441 aaataaaaca atctttgttg ccgtttaaat aatgtgaatg tcaataaatt ctccaaacta
     1501 gtcgaaaata aaacaggctg ggacataaat ccc
//
LOCUS       DAHHYJ010000240         1516 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000240 DAHHYJ010000000
VERSION     DAHHYJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.246"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(33..236)
                     /locus_tag="NBK22_002621"
     CDS             complement(33..236)
                     /locus_tag="NBK22_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415772.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            complement(265..1074)
                     /locus_tag="NBK22_002622"
     CDS             complement(265..1074)
                     /locus_tag="NBK22_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD2415773.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
BASE COUNT          471 a          257 c          205 g          583 t
ORIGIN      
        1 tcatattagg cttcatttca ataattatgc gtttaaactt ctttgaaata tgttttatag
       61 taaccgccaa ctaatccaga attatctatt atttgataat attcttcagt ttcgttgata
      121 acatcatatt gtttgccaac cgttaacatg tcagaaactg taaatttctt tgcatctgta
      181 ttaatgactt ctactttctt gattggtgta cgttctttcc aagtttcatg taacataaat
      241 tttgtcctca ctatcgtaaa aaaattattt ttgtaaagtg aatataaacg ataatccact
      301 gttttcagta attctaattg attcaatatc atcttgctca agcgtttgat atggtatttc
      361 cacttcatct aagcgcgatt tcgcttgatt aaagaaatct gcatctttcg tttcaaactc
      421 aacttgatcg actgcgccat attccggcat tgtcatttct gtttctacag gcattaaatg
      481 tatttcgcca cctagaccac ccgttccaac tttgaatact tggacatgat agtcagcatt
      541 gtcgaagggt tggtattctg caaatacttc aagtccgaat atatttgtta aaatttgacc
      601 tgtaatatct acatgattca ctttaagaat cactggtcct aaaccttgca cttgatgtaa
      661 cggattgacc gcactctcaa aagaaggcat acctaaacca acgccataat tattctcgtt
      721 tgaatatatt gagaaaatat gaccattgtt atcttcgaga ctgaaatatt tattgccatt
      781 taattcttta actgttgtaa atggaatatc cttattcgat aatatttccg catactcctc
      841 taaacctgag tcagttggtg tacgtaatcc aatacccaca aagtgagatt cttctaattg
      901 ctcacttgga acttgtataa attgaagtct tgttcctggg cttatatctg catcgccgaa
      961 acgaattgag ttttcaagaa gttcttcata atttaatcct aatatgtcaa ccatgaaatg
     1021 ttttgtctgt tctatatttg ttgtacctaa tgttatactt ctaagtccac acattatagc
     1081 cacctctatc tttatcttta ctattatatt ataacttatt ttaatcataa attatagaat
     1141 atcggtcagt tgattttgat tatagattaa tatgtgaaaa atgtatttct ttaaaagaac
     1201 tatttcattt aaataggtaa cataatattg ttatggttgc tattgagtat gtcattaatg
     1261 agtatctgta tatagcaatt gaatatttcg ttttatgtag tacataaaaa acatatacat
     1321 gtcgtatcca taaagtgaat ttacatcttg ttcagatgat tttaaattaa ctaaaggcgc
     1381 ttatagaatt cataaatttc atcgcttgtt attatctaaa atgatttcat tagcatattg
     1441 aaacacgcca ttccacctat atttctattg tgagtctatt tcaataaaaa tgatatattt
     1501 tttaagttgt caaaat
//
LOCUS       DAHHYJ010000241         1515 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.247, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000241 DAHHYJ010000000
VERSION     DAHHYJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.247"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            395..499
                     /locus_tag="NBK22_002623"
     CDS             395..499
                     /locus_tag="NBK22_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415774.1"
                     /translation="MFKVNYSILSYYPEYNIAVSWQRLREGKTIKNKI"
     gene            628..879
                     /locus_tag="NBK22_002624"
     CDS             628..879
                     /locus_tag="NBK22_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415775.1"
                     /translation="MLTLIKLKEDEQVIIYEYIPEDDISNGKGSVTFNKKDAEVIDFS
                     LSEIENEEYFMLYRNKSFSVVRDFIEKQEFPENYKIAWY"
     gene            952..1158
                     /locus_tag="NBK22_002625"
                     /pseudo
     CDS             952..1158
                     /locus_tag="NBK22_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            1354..>1515
                     /locus_tag="NBK22_002626"
     CDS             1354..>1515
                     /locus_tag="NBK22_002626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCD2415776.1"
                     /translation="MLKDAKENNDSNEVAYLLKDGKVTKVYGDQDSVSFAPGEKATEL
                     LFNSKPNSIV"
BASE COUNT          602 a          182 c          267 g          464 t
ORIGIN      
        1 gaaaaaggag tatgtcgttt catttagaat ttattgcacg aaattagcat cgaataaggg
       61 aaaataagtg ttaagtttta aatgataaaa aagattggaa tggatcgtct tgaaatgctc
      121 ccttcaaagt tttcattttt tcaatgtcga cttcgaaggg ggcattttca ttaaattgtt
      181 atagcttttt atatttgtat aatgaacata taagtttaag aaggtgcgag tgaaggaaat
      241 aaaaaagctc aaatgtacca aattgttaat cttaataaat ctctacttta taaagattga
      301 atggacattc gagcgttaat cagtcaggag ggaatttcac tcctacaatt taataataat
      361 acttgcttca ccactgtaca aggagtgatt tgttatgttc aaagtgaatt attcgatttt
      421 aagttattat ccagaatata atatcgcagt aagttggcaa cgtttaagag aaggaaaaac
      481 aataaaaaac aagatttaat actgctgcgt catgaggcgc ttgaacatta tttgatgaat
      541 aagtataatt tcaactatga ttatgcacat aaaattgtat caaaaaaata cgattattca
      601 atttttataa aaaagaaggt ggattaaatg cttacattaa taaaattgaa agaagatgaa
      661 caggttataa tatatgaata tatacctgaa gatgatataa gtaacggtaa aggttcagta
      721 acttttaata aaaaagatgc agaggttata gatttctcat tatctgaaat agaaaatgaa
      781 gaatatttta tgttatatcg taataagtct ttttctgtag taagagactt tatcgagaag
      841 caagaatttc ccgaaaatta taaaatagca tggtattaat gcatcctttc tagacagata
      901 aagagaaagt ggtgctattt ttatatgctt ttttaactat ttactgaaat agcagaatca
      961 caaaagttaa catatttaaa agaacttggt gaagatggcg aatataaata tgttgccaaa
     1021 atagatagta aaacgtctaa actatgtcat tcgctcaacg gcaaagtatt taaagttaaa
     1081 gatatgatac caggtgtgaa tgcgccacct atgcatcctt ggtgtagaag taccacagtg
     1141 ccacatgttg gcaattggtg agacaagttc tttaaagagc gtgaaggtaa atatcaagta
     1201 gaagatgaca caaccaaaga tgaattacaa caagctaaag tattaggaaa gaaaatatat
     1261 attactgatc aagcaattga taaagtcaga tatgttgata ttccaacaca taccaaagaa
     1321 gaaaatcaat ttatacaaga acaacataaa gccttgctta aagatgccaa agaaaacaat
     1381 gatagcaatg aagtagctta tttattaaaa gatggtaaag ttacaaaagt atatggtgat
     1441 caagatagtg tatcttttgc accaggggaa aaagcaacag aattgttatt taacagtaaa
     1501 ccgaattcaa ttgtt
//
LOCUS       DAHHYJ010000242         1514 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.248, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000242 DAHHYJ010000000
VERSION     DAHHYJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.248"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..563)
                     /locus_tag="NBK22_002627"
     CDS             complement(<1..563)
                     /locus_tag="NBK22_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD2415777.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYK"
     gene            674..1514
                     /locus_tag="NBK22_002628"
                     /pseudo
     CDS             674..1514
                     /locus_tag="NBK22_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
BASE COUNT          584 a          180 c          236 g          514 t
ORIGIN      
        1 attttgtatt tttcaaataa agatgcctca gttagaagat gattattctc tttattggga
       61 gcaatcaata caattttacc ttcgcatact ttttcagaat gaacttctct tagtttaggt
      121 tgatttctgc taatcccgat gtcatacgta tgattattaa tatctttttc aatattttca
      181 tttttgacat gaagagaaac atcgataaaa ggatgctcgt taaagaaaga ttttaaaaat
      241 ttgggcataa tgaatgtcgc gatatatgaa gacacgacaa catttaattt cgattgaaac
      301 atcgtttttt taagttggat atgtttgatg ccactttcat attgttcaat aaaactttgc
      361 gcaattggaa gaaatgtatg accatcttca gtcaagataa tttgattttt ataagtttca
      421 aatagtttca cattgagatg ctgttctaaa tttttaattt gcttatgtat agaaggtata
      481 gtgagattaa tttcttcact agctaatcga tagtttaacg tcttcgctaa aatgacaaat
      541 gtatagtacc aatctaaatt catgaactta cctctttcaa aaaagttaat aattcattag
      601 ataatattta atttttattg cataaaaata atattaatat tatatcagag gttatcgagg
      661 ggggtgaagt aatatgattc gaattcaaga taatacaata agggatggta tgcaacaaag
      721 taatgttcga aaaagtctaa ttataaaaaa agaagtattg aaacaaatta acaagttaaa
      781 tataaattct gttgaagtag gcatgtgtac aactatcgag gatgaattta atattcatca
      841 attcagagac attttaagtc ctgaaaaaga attagtagta ttgactaggc ttaatgaaaa
      901 agaaataaaa aaatagtcaa attgaaaatt cataatttag tggtaaaaat actattgcca
      961 atatctgact tgcatataaa agaaaagctt aatttttcaa ataaatatta tattcagaaa
     1021 atcaaagact gcttggatat attaaagaaa gataaaaaag gagtagatat ttgttttgaa
     1081 gatgcaacaa ggacttctag agaaaaattg aaagaataca tggaaattat ttcaaaatat
     1141 caagttagaa cagttacatt tgcggacact gtaggatgtt cgacaccatt agaatacgga
     1201 gatattttta attactttgt aaaaaaatat tctaacataa ttttttctgc tcattgtcat
     1261 aacgatctag ggttggctac tgcaaataca ttagctgcaa ttttaaatgg tgcaaagcaa
     1321 atagaaacta catttttggg aattggtgag agagcgggta atgctcctat tgaggaaata
     1381 attactattt tgacaaaaaa acaaatagaa agtacggaat tcactttacc cgacgtatat
     1441 aaaactagta ttaatatttc taaaattctc gattttcaaa tatcagaaaa caaacctata
     1501 attggtgaaa atat
//
LOCUS       DAHHYJ010000243         1494 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000243 DAHHYJ010000000
VERSION     DAHHYJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.249"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            186..1028
                     /locus_tag="NBK22_002629"
     CDS             186..1028
                     /locus_tag="NBK22_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD2415778.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVLGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDNGENIDAYILRETL
                     VDNN"
     gene            complement(1153..1464)
                     /locus_tag="NBK22_002630"
     CDS             complement(1153..1464)
                     /locus_tag="NBK22_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD2415779.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
BASE COUNT          530 a          200 c          269 g          495 t
ORIGIN      
        1 aatttaaaat tacaggccga tggttatgat gcatttacat ataatatgat tgcattaatc
       61 tttataagta gttagaagtg tattagtgtg gggcgtttcg tttttgttcg catttatgaa
      121 tagtgatatg atgtaataaa cacatgctaa ttgtaataac gaatacagaa agaataggtg
      181 atgttatgaa aatcggggat atatctattc attatctaaa tggtggcaat acaaaaatgg
      241 atggcggtgc aatgtttggt gttgttccga agccgttgtg gtcaaagcaa tacaatgcaa
      301 atgaacgaaa tcaaatcaat ttaccgacac atccaatttt gattcaaacg gctcaatata
      361 atttgattat agatgcaggt attggtaatg gtaaattatc tgaaaagcaa ttacgtaatt
      421 ttggagtaga tgaagaaagt catataattg ctgatttggc aaattataat ttaacgccaa
      481 aggatattga ttatgtgcta atgacacata tgcattttga tcatgctgcc ggtttgactg
      541 atcaagcagg acatgcaatt tttgaaaatg cgattcatgt tgtgcaacaa gatgagtggc
      601 atgagtttat tgcacctaat ataagaagta aatcaactta ctgggataag aataaaggcg
      661 attatagtaa caagttgatt ttattcgaaa aacattttga accggttctg ggtatcaaga
      721 tgcaacatag tggaggtcat agctttggcc acacgattat tacgattgaa agtcaaggag
      781 ataaagcagt tcatatgggt gatatattcc caactactgc acataaaaat cctctatggg
      841 taacggcata tgatgattat cctatgcaat cgattcgtga aaaagaacgc atgataccat
      901 attttattca gcaacaatat tggttcttgt tttatcatga tgaaaactac tttgctgtaa
      961 aatacagcga taatggtgaa aacatagatg catatatttt acgtgaaaca ttagttgata
     1021 ataactaaaa taaagatgta ttactaaaca aattttcaaa aataaaaaat tgagccacat
     1081 ccaatcttac taattagggt gtggctcatt tttaagtttt acgatccaaa tcaaatatgg
     1141 ataaaattcg tattaacgct ctacaatgtt aatgacttca ccagtatatg catctgcata
     1201 aaaatcataa tgaatatttt gaccattttt aatagttgta attccacctt gataaactaa
     1261 acggtattta tcagtttcag gatgaacgaa tggttcataa acgatatatg agcctttgac
     1321 attcataaaa tatgatttta cttcattaag tactttaacg ggattatagc gtttacgatt
     1381 aataaattgg atgattgaaa tggtagaaat aattacaatg gctactgcaa ttattgttgg
     1441 aattatatat ttcagtttag tcatcattta tgctcccttg atttcaaatt cata
//
LOCUS       DAHHYJ010000244         1451 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000244 DAHHYJ010000000
VERSION     DAHHYJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.250"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..1192)
                     /locus_tag="NBK22_002631"
     CDS             complement(<1..1192)
                     /locus_tag="NBK22_002631"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD2415780.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGMNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYD"
BASE COUNT          439 a          278 c          223 g          511 t
ORIGIN      
        1 cgtcatatag tttttctacc gcttgttgcc cattaaaatc atcaatgacg tatgaaatac
       61 ttatttcaga tgttgttgtt tggtagaaag gtatattatt ttcaattaat gtcaaaaatg
      121 cttttgatgc cacacctgac atatcacgca tgcctgagcc aattaatgaa attttgacat
      181 aatgctcatt gattttataa gctaatgctt catattgatt ctttaatgtt tcaagaatca
      241 tagaaatttg atgaaaatca ctatctttaa tcgtgaagga tagttgtagc ccatccaagt
      301 tgacgatttg tgaaatcata tcaacattta cagcaccttc ttcaagttcc gtaaatagtt
      361 gggtaagtag ctgattgtca ggtaggggat aactaattgt tacatgcatc atatgtttat
      421 ccaaagccac accagtaact gcttttttct ctaatatttc ttcatttgac ataatccatg
      481 ttcctttcac gttcgataaa gtttttccta aatataaagg gatattatag tttttagcta
      541 attcaacact tcttgtttca agtacaccag cacctaaagc gctcatttcc atcatttctt
      601 catatgagac gatgtctagt cgtttagcct ttggtaaaag tcttgggtca gtggcataca
      661 caccatcaac gtcggtataa atttcacaag gtatttgatt actaacagca agtgccacag
      721 cggtcgtatc agaaccacct ctgcctaaag ttgttaattc ctgatgttca ttgatgcctt
      781 gaaatccagc aactactaaa atatcgtttt cttgaaaggc ttgttcaaat gtttgaggat
      841 taatttgagc aattttactt tttaaatgat ggccaatggt tttaataccc gcttgatagc
      901 cagtcattgc tttggcattc ataccgatat catttaatac cattgataaa taagatacag
      961 tttgttgctc tccggttgtc aataataatg ccagttcttg ttgttttggt gctttagtca
     1021 aggtcgatac attcgtcatt aattgatctg ttgtgttacc catagcactt acaacgacaa
     1081 ttaattgttc atcttgattg actcgctcct ttaacatttc agcgatcctt tttatttttg
     1141 taaaatcact gacggatgat ccgccaaatt tcaacacact tcttgttacc atataatcct
     1201 cctaattgtt aggaagggca cacaataata aaaaaagaac aagttcggaa tgcgaacttg
     1261 ctcttaatct atatacaagt gatgcactcc attattttta aataatgaca aactctcagc
     1321 tcttaaccaa aaagtccaac aaattataac tgctattata attgcttcgg catcgcaccc
     1381 tttcaaattt agctgttagc agacagtaat ctaaacttta ctcatgattg atgcgcctca
     1441 tcaaaatatt a
//
LOCUS       DAHHYJ010000245         1443 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000245 DAHHYJ010000000
VERSION     DAHHYJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.251"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            274..774
                     /locus_tag="NBK22_002632"
     CDS             274..774
                     /locus_tag="NBK22_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD2415781.1"
                     /translation="MTFEEKLSEIYNKIANEISGMIPVEWDQVFTIAYVNDRGGEVVF
                     NYTKPGSDELNYYTNISRDYNVSEEIFDDLWMNLYYLFKNLRNLFKAEEHEPWTSCEF
                     DFTRDGKLNVSFDYIDWIKLGLGPLARENYYMYKKFGVIPEMEYEMEEIKEIEQYIKE
                     QEETKL"
     gene            785..1285
                     /locus_tag="NBK22_002633"
     CDS             785..1285
                     /locus_tag="NBK22_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD2415782.1"
                     /translation="MTFEEKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKPGSDELNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKEEELEPWTSCEF
                     DFTRDGKLNVSFDYIDWVNSEFGPMGREHYYMYKKFGIWPEKEYAINWVEKIKDYVKE
                     QDEAEL"
     gene            1296..>1443
                     /locus_tag="NBK22_002634"
     CDS             1296..>1443
                     /locus_tag="NBK22_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF600 family protein"
                     /protein_id="HCD2415783.1"
                     /translation="MTFEGKLSQMYNEIANEISGMIPVEWEQVFTIAYVTDQAGEVIF
                     NYTKP"
BASE COUNT          564 a          137 c          298 g          444 t
ORIGIN      
        1 taaatagtta aataaattga aagacaaaat aatgagtagg agttttaggg ttattattta
       61 caaataatat aatactaaaa aagactcgaa attatttgga attccagtta tcgtaacaat
      121 agttgcagtt attttagttc cattagtgaa tttaggtatg aaatgagtaa tattaatttg
      181 aagaagcatt aagcgacgta gaggtgttgt ttagtgcttt ttatttaacg gaaatgaaac
      241 caatatctat tttgaaaata taggggcgat aacatgactt tcgaagaaaa actaagtgaa
      301 atatacaaca agattgcgaa tgagattagt gggatgattc cagtagaatg ggaccaagta
      361 tttacgatag cttatgtaaa tgatagaggt ggagaggtcg tttttaatta tactaaacca
      421 ggtagcgatg aattgaatta ttacacgaat atatctagag attataatgt ttcggaagaa
      481 atatttgatg atttatggat gaatctgtat tacttgttta agaatttaag gaatttattt
      541 aaagcagaag aacatgaacc atggacatca tgcgaatttg actttacaag agatggtaaa
      601 ttaaacgtat cttttgatta tattgattgg attaaattag gcttaggtcc attagctaga
      661 gaaaattatt atatgtataa aaaatttggg gttataccag aaatggaata cgaaatggaa
      721 gaaattaaag aaatcgagca atatattaaa gagcaagaag aaactaaact ttaggggaga
      781 taatatgact ttcgaagaaa aattaagtca aatgtataac gagattgcga atgagattag
      841 tgggatgata ccagtagagt gggagcaagt atttacaata gcttatgtaa ctgatcaagc
      901 tggagaagtc atctttaatt atactaaacc aggtagtgat gaattaaatt attattcaga
      961 catacctaaa gattgcaatg tctcaaaaga tatttttaag aattcatggt ttaaagttta
     1021 tcgaatgttt gatgagttaa gagaaacttt taaagaagaa gagcttgaac catggacatc
     1081 atgcgaattt gactttacaa gagatggcaa attgaatgta tcttttgatt atattgattg
     1141 ggtgaattca gaatttggac caatgggaag agaacattat tatatgtata aaaaatttgg
     1201 aatttggcct gaaaaagaat atgccataaa ttgggttgaa aaaataaaag attatgttaa
     1261 agagcaagat gaagctgaac tataggggag ataatatgac tttcgaagga aaactaagtc
     1321 aaatgtacaa cgagattgcg aatgagatta gtgggatgat accggtagaa tgggagcaag
     1381 tatttacaat agcttatgta actgatcaag ctggagaagt catctttaat tatactaaac
     1441 cag
//
LOCUS       DAHHYJ010000246         1407 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000246 DAHHYJ010000000
VERSION     DAHHYJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.254"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(136..1197)
                     /locus_tag="NBK22_002635"
     CDS             complement(136..1197)
                     /locus_tag="NBK22_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCD2415784.1"
                     /translation="MSNANNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAFIVHELC
                     KKFIPIYSNDIYKFEVPKQ"
BASE COUNT          550 a          249 c          165 g          443 t
ORIGIN      
        1 aaaaaatccg ctattatacg tgaacaaatc tcacatttta aaatcaatca aaaaagcgct
       61 actatcaata tcttattgac agtaacgcct atatttatca tatattaaaa cttattcttg
      121 tcatcaagct ataaattatt gttttggaac ttcaaattta taaatatcat tagaatatat
      181 aggaatgaat tttttacaca attcatgaac aataaatgcc gatacaaatg gtatgacaaa
      241 ataaccaagt gctaataaaa taatattcat tgttggatca cctgacattc ggttaaatgc
      301 atttattggg ccaactaatc ctgtatatcc aaatccagca gacaatggtg ttcctttaac
      361 ctgaagaacg taagcaatga ttccagtaat tattccattt atagtcaatg gtatcgaaat
      421 aattaaattt ttcaagtaaa ctgggatcat catttttgca gctcctatga gtaaaactgc
      481 atttacacca atagaattga cacgcaatga gccaaataag aaagtaacac aggcagccac
      541 tatacctagg tttgctgctc cacttcctag tccgtttaaa ctaatcgcag ttgcaatcgc
      601 tactaacgat attggtgtta ccattaataa tgaaaatgcc acactaataa gtatagacat
      661 taacaacgga tttaagtctg taaaagaatg aattacattt ccaattgctt gagtaatttt
      721 tcgaatgtaa ggtaatgtga ttagaccgat acccccacta acgataggta ctaaaactgg
      781 taatataatt aattcaaaag atccaagttt gttttgtaat accatatata taagacatgc
      841 aataataaca accaaactcg tatttataat gtcacctata ccttttaaca taaaactatt
      901 attactatat acaacagcac ctgaaccaat catagctgat gtacctacta tagcagcacc
      961 tgcgccatta aatttaaatt gatgagcagc taaaacccca ataataaatg ccataaatga
     1021 ttgaattagt atcactaact gatacgttaa ttctaaaatt tcattaccac ttttaaatat
     1081 ttttaatact tcacctaata gagcattcgg aacaagtgca ataacaacac cagcaccaat
     1141 agaatttaaa atcttactga aaaactgttt attatcagca ttatttgcgt tactcataaa
     1201 cgacctccaa tttgaacatt aaactcatca tattatagaa aattaacttt aacaatgatt
     1261 agttggctaa tttaatctaa atataacaat ccacaaaatg aataaagtaa atttaaacaa
     1321 tctactcact aatcaatgtc atgctaatct taaaatacta ctaattacat cctgtgacta
     1381 ataatgtaat aaattgtaaa tattaaa
//
LOCUS       DAHHYJ010000247         1402 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000247 DAHHYJ010000000
VERSION     DAHHYJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.255"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            70..>1402
                     /gene="sasG"
                     /locus_tag="NBK22_002636"
     CDS             70..>1402
                     /gene="sasG"
                     /locus_tag="NBK22_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored surface protein SasG"
                     /protein_id="HCD2415785.1"
                     /translation="MQMRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYL
                     GTQQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVTSNKDTIEH
                     EPSVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSK
                     NVVESTPITIQGKEHFEGYGSVDIQKKPTDLGVSEVTRFNVGNESNGLIGALQLKNKI
                     DFSKDFNFKVRVANNHQSNTTGADGWGFLFSKGNAEEYLTNGGILGDKGLVNSGGFKI
                     DTGYIYTSSMDKTEKQAGQGYRGYGAFVKNDSSGNSQMVGENIDKSKTNFLNYADNST
                     NTSDGKFHGQRLNDVILTYVASTGKMRAEYAGKTWETSITDLGLSKNQAYNFLITSSQ
                     RWGLNQGINANGWMRTDLKGSEFTFTPEAPKTITELEKKLKRFHSRKNVNLI"
BASE COUNT          564 a          186 c          279 g          373 t
ORIGIN      
        1 ctcctattgt atttgtaaaa ttacatgtaa attcactgta agtaaagtgg aaaatatgga
       61 aaaaggagta tgcaaatgag agataagaaa ggaccggtaa ataaaagagt agattttcta
      121 tcaaataaat tgaataaata ttcaataaga aaatttacag ttggaacagc atctatttta
      181 attggctcac taatgtattt gggaactcaa caagaggcag aagcagctga aaacaatatt
      241 gagaatccaa ctacattaaa agataatgtc caatcaaaag aagtgaagat tgaagaagta
      301 acaaacaaag acactgcacc acagggtgta gaagctaaat ctgaagtaac ttcaaacaaa
      361 gacacaatcg aacatgaacc atcagtaaaa gctgaagata tatcaaaaaa ggaggataca
      421 ccaaaagaag tagctgatgt tgctgaagtt cagccgaaat cgtcagtcac tcataacgca
      481 gagacaccta aggttagaaa agctcgttct gttgatgaag gctcttttga tattacaaga
      541 gattctaaaa atgtagttga atctacccca attacaattc aaggtaaaga acattttgaa
      601 ggttacggaa gtgttgatat acaaaaaaaa ccaacagatt taggggtatc agaggtaacc
      661 aggtttaatg ttggtaatga aagtaatggt ttgataggag ctttacaatt aaaaaataaa
      721 atagatttta gtaaggattt caattttaaa gttagagtgg caaataacca tcaatcaaat
      781 accacaggtg ctgatggttg ggggttctta tttagtaaag gaaatgcaga agaatattta
      841 actaatggtg gaatccttgg ggataaaggt ctggtaaatt caggcggatt taaaattgat
      901 actggataca tttatacaag ttccatggac aaaactgaaa agcaagctgg acaaggttat
      961 agaggatacg gagcttttgt gaaaaatgac agttctggta attcacaaat ggttggagaa
     1021 aatattgata aatcaaaaac taatttttta aactatgcgg acaattcaac taatacatca
     1081 gatggaaagt ttcatgggca acgtttaaat gatgtcatct taacttatgt tgcttcaact
     1141 ggtaaaatga gagcagaata tgctggtaaa acttgggaga cttcaataac agatttaggt
     1201 ttatctaaaa atcaggcata taatttctta attacatcta gtcaaagatg gggccttaat
     1261 caagggataa atgcaaatgg ctggatgaga actgacttga aaggttcaga gtttactttt
     1321 acaccagaag cgccaaaaac aataacagaa ttagaaaaaa agttgaagag attccattca
     1381 agaaagaacg taaatttaat cc
//
LOCUS       DAHHYJ010000248         1370 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000248 DAHHYJ010000000
VERSION     DAHHYJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.256"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            97..447
                     /locus_tag="NBK22_002637"
     CDS             97..447
                     /locus_tag="NBK22_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCD2415786.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     MLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTHG"
     gene            440..1003
                     /locus_tag="NBK22_002638"
     CDS             440..1003
                     /locus_tag="NBK22_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCD2415787.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTSYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            1017..1361
                     /gene="gpsB"
                     /locus_tag="NBK22_002639"
     CDS             1017..1361
                     /gene="gpsB"
                     /locus_tag="NBK22_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCD2415788.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
BASE COUNT          551 a          171 c          235 g          413 t
ORIGIN      
        1 acatttgaaa aaatagttaa atcaaattat atagagtgtt aaaacatgac gaatacagta
       61 tattgtgata aattaaaaaa tgtaggaggc gtcctcatga atgatgtagt cgaatcacta
      121 atttatgaag ttaacaacat gcaacaaaat tttgaaaatg tgaaatcaca acaacaagat
      181 catgattttt accaaactgt aaagccatat actgaacata ttgatagcat gctcaatgag
      241 atcaaattac atcgtgaatt tattatagaa gtaccttata tgaattcaag gaaatttgag
      301 ctactgattg ctaacattga acaactttct gtcgaatgtc attttaagcg aacaagtcga
      361 aagttattta tagaaaagct taaaagtgtt caatatgatt tacaaaatat attagatggc
      421 gtaacaaaag agggtactca tggttaaaac agtttatgta acaggttaca aatcattcga
      481 attaaacatt tttaaagatg acgcacctga agtacattat ttaaaacaat ttataaaaca
      541 taaaattgaa caactgttgg atgaaggatt agaatgggtg ttaatacaag ggcaaatggg
      601 tatagaatta tggactgcag aagttgttat tgaattacaa cgaacatatg attcgttaaa
      661 gtttgctgta attacaccgt tccaaggaca tacagaaaag tggaatgaac ataatcaaag
      721 taagtatgcc aacataatta agcatgcaga ttatgttgat agtatttttc atacgtcgta
      781 tcagggtcct tttcaattta aacaagcaga tcaatttatg cttgaacatt cggatcaaac
      841 attgctcatt tatgatgagg aacaagaggc aagtcctaag ttcttcaagc agatgttagt
      901 tgattttatg gataaaacaa actatacttg tgatattgtg acgttcgatg aattaacagc
      961 attcatcaat gacttacagt ggtctgaaga tcaaagtttc taatgaggtg gaaaaaatgt
     1021 cagatgtttc attgaaatta tcagcaaaag atatttatga aaaagatttt gaaaaaacga
     1081 tggctcgtgg ctatagaaga gaagaagtag atgcattttt agatgacatt attgctgatt
     1141 atcaaaaaat ggccgatatg aataatgaag ttgtaaaatt atcagaagag aatcataaac
     1201 ttaaaaaaga attagaagaa ttaagactac gtgtagcaac atcaagacct caggacaata
     1261 aaagtttttc ttcgaataat acaacaacaa atacatcttc aaataatgta gatattttaa
     1321 aacgtatttc aaacttagaa aaagctgtat ttggtaaata attactaaat
//
LOCUS       DAHHYJ010000249         1367 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000249 DAHHYJ010000000
VERSION     DAHHYJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.257"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..932
                     /locus_tag="NBK22_002640"
     CDS             <1..932
                     /locus_tag="NBK22_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415789.1"
                     /translation="LLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKESEEEKDPDL
                     EKYEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKVELADYRFT
                     KDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNSNTPVFYAE
                     IMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKPVPYSVAQI
                     KSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLNLVHMDFLN
                     ASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1095..1298)
                     /locus_tag="NBK22_002641"
     CDS             complement(1095..1298)
                     /locus_tag="NBK22_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCD2415790.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGILHLIFCWTGIPH
                     ILAIISAVITVFKPADEQGNVTL"
BASE COUNT          530 a          190 c          225 g          422 t
ORIGIN      
        1 tacttttaat tatgctttta gtcgtagcag gttgttcttt tggtgggaat cataaattat
       61 catcaaagaa atcagaagaa tcaaaacaag aaactgtaaa aaaagaatcg gaagaagaga
      121 aagatccaga tttagagaaa tatgaagaaa tagagaagaa aatgaaagga attaaagatg
      181 cgccatctct tgataagttg gatccattaa tgacagaaaa gtcgtttacg aatagtaaag
      241 ggattcaagg atggaaagat tacaaagaat taatgggtaa agtggaactt gcagattata
      301 gatttactaa agattcaaaa ggatcttcaa taaaagatgt tgatgcattc tttaaaggta
      361 agaaaggtat aaaaaggaaa gtgattgaaa cacacgatga tgtaaaacaa gttgattatt
      421 ggtatgtaga tccagatgga aagaaaattg gcaattcaaa cacacctgtt ttttacgcag
      481 aaattatgac aaaatataaa gatggaaagt tagtttatgc atcagtcgaa ccaggatctt
      541 acgtaataca taaagatgat gcaattaaat atgacgatta ttctaagtta aaaaaattaa
      601 gtcagctaac taaacttgat catccaaaac cagttccata tagcgtagct caaatcaaat
      661 ctttcggagt acctttaaca agcgtttcat ttatgacaca tggatcaaag gatactaaag
      721 atgaagtgtt gccggcattg gcctatttca ctttttcacc aaaaaattat gaagacaagt
      781 ctaatccaga tccaaaagtt ttaaatttag tacatatgga tttcttaaat gcatctagtg
      841 attttggtaa cgcacatttt gttgttttaa gtaaatatat taaagagtat gaatcaaact
      901 atgaaacagc gtcagatgat tctttaaaat agtatttact gtgtgaaaaa taaatagtgt
      961 actacattaa ataatcgcaa taataatccc gataaacaat cagcattact gcttatcaca
     1021 tagagttcgt aataactata actctatgat tcgcaaataa taaatgattg tcatcgggat
     1081 ttatttttat caatttataa agtgacatta ccttgttcat cagcaggttt gaaaacagta
     1141 atcactgcac taataattgc taaaatgtgt gggatacctg tccaacagaa tattaagtgt
     1201 agaatacctt gcatattctt gccggcataa aatttatgaa taccaaaact acctaagaac
     1261 aatgctaata aaatataaat aactttgttt acttgcattt ctttccctcc agttgaattg
     1321 cttataatga cattagcttc tctttttatt atacccactt ttagttc
//
LOCUS       DAHHYJ010000250         1365 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000250 DAHHYJ010000000
VERSION     DAHHYJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.258"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            185..490
                     /locus_tag="NBK22_002642"
                     /pseudo
     CDS             185..490
                     /locus_tag="NBK22_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            581..>1365
                     /locus_tag="NBK22_002643"
     CDS             581..>1365
                     /locus_tag="NBK22_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD2415791.1"
                     /translation="MFKRTKLILIATILLSGCSTTNNESNNETKSVPEEMEASKYVGQ
                     GFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVHCEDH
                     GIVFNASLPLYKDAIHQKGSMRSNDNADNMSMMVGTVLSGFEYRAQKEKYDNLYKFFK
                     ENEKKYQYTGFTKEAINKTQNVGYKNEYFYITYSSRSLKEYRKYYEPLIQKNDKEFKE
                     GMERARKEVNYAADTDAVATLFSTKKNFTNDNTEYDVIELSDK"
BASE COUNT          564 a          172 c          236 g          393 t
ORIGIN      
        1 accagtatta aactacattc ttgaagttat acattcggta gaaacagctg tgagacatat
       61 ttatgaaata agcagtttaa tatacaaatc aggaatgaaa ataaagataa aggcaatggg
      121 aataaatgtt gacgaaattg ataaaatgct tgaagtttta aaaagtaaac ttaaaaactt
      181 agacgaattt ttaaataatt taaaaatagc ttcgccgatt ttagaaaatc acttagatga
      241 tatagttaga aatatgaaac cgttaattgt taatcagatt tttgaaccat cacattatga
      301 cgatatgttt attttaaaca cacaagctca tgctcgtctt gatcaaatgg cacaacagtt
      361 tgaagttgtt tgtaatggct tgaacgaaaa tgaaggacaa gctattcaaa caatggatca
      421 atctgcctcg ttaattcgtt caaacttaat tcaagttaaa gaacaattag aaaaactagc
      481 agtttactaa ttaatttatt aaaagctact tgttttcatt gagaataagt agctttttca
      541 aacacaaaag ttttataaac acataaatgg gtgatgagct atgtttaaaa ggacaaaact
      601 aatcttaata gcaacgatac tactatcagg atgttcaact accaataacg aatccaacaa
      661 tgaaacaaaa tcagtaccag aagaaatgga agcttcaaaa tatgtaggac aaggcttcca
      721 accacctgca gaaaaagatg cgattgaatt tgcgaagaag catcgtaaag aatttgaaaa
      781 agtaggtgaa caattcttta aagataactt tggactaaaa gttaaagcta caaatgttgt
      841 aggtaaagat gatggtgtag aagtttatgt gcattgcgaa gatcatggca ttgtatttaa
      901 tgcaagtcta cctttgtaca aagacgccat ccatcaaaaa ggttcaatgc gtagtaatga
      961 caatgctgat aatatgagta tgatggtggg tacagtgctg agtggctttg aatatcgagc
     1021 gcaaaaagaa aagtatgata atttatataa attttttaaa gaaaatgaaa agaaatatca
     1081 atatacaggt tttactaaag aagcaattaa caagacccaa aatgtcggat ataaaaatga
     1141 atatttttat attacatact cttctagaag tttaaaagaa tatcgaaagt attatgaacc
     1201 tttgattcaa aaaaatgata aagaatttaa agaaggaatg gaacgagcaa gaaaagaagt
     1261 gaattatgcc gctgatacag atgcagtagc gacactgttt agtacaaaga aaaattttac
     1321 taatgacaat acagaatatg atgtaattga actaagtgat aaatt
//
LOCUS       DAHHYJ010000251         1357 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.259, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000251 DAHHYJ010000000
VERSION     DAHHYJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.259"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..973
                     /locus_tag="NBK22_002644"
     CDS             <1..973
                     /locus_tag="NBK22_002644"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD2415792.1"
                     /translation="NNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQN
                     TIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTEPIV
                     QALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNALKV
                     NPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDWE
                     NIEKLSNDKYRTWAWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFG
                     VGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS"
     gene            complement(1054..1272)
                     /locus_tag="NBK22_002645"
     CDS             complement(1054..1272)
                     /locus_tag="NBK22_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCD2415793.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
BASE COUNT          498 a          178 c          233 g          448 t
ORIGIN      
        1 taataataat attacagatc caactttaaa cttagcaatg gaagaatatg ttttaaaaaa
       61 tttaccagca gaagaaagtt actttttatt ttacataaat agaccatcta tcattgttgg
      121 aaagaatcaa aatacgatag aggaagtaaa tcagacatat atagatgctc ataacatcga
      181 tgtagttaga agaatttctg gtggtggcgc tgtttatcat gatactggca atttaaactt
      241 tagttttata acagatgatg atggtaacag tttccacaat ttccaaaaat tcactgaacc
      301 aattgttcaa gcattacaat ctttaggcgt aaatgctgaa ttaactggtc gtaacgatat
      361 tcaagttgga caagcgaaaa tctcagggaa tgctatggtt aaagttaaaa atagaatgtt
      421 tagtcatggt acgttgatgt taaatagtga tttagacgaa gttcagaatg cactaaaagt
      481 taatccagct aagattaaat caaaaggtat taaatctgtt cgtaaacgag tagcaaacat
      541 tcaagaattt ttaaatgacc ctttagaaat tgaagaattt aaaaaaatca tcttgaaaac
      601 tatctttgga gaaactgaag tagaagaata taaattaacg gatgaagatt gggaaaatat
      661 tgaaaagtta agtaacgaca aatatagaac ttgggcatgg aattatggta gaaaccctaa
      721 atataacttt gaacgcgagg aaaaatttga aaaaggattt gtacaaatta agtttgatgt
      781 taaacgaggt aaaatcgaac atgcaaaaat tttcggtgat ttctttggtg tcggagatgt
      841 cactgatctt gaaaatgcat tagttggctg tctacacgac tttgagcaca tagaagaagc
      901 actatcagaa tatgatttat atcattattt cggtgacata gatagacatg aattaattag
      961 attaatgtca taaacgatta aaggactatt cttacatgta tgagatgtta agaatagtcc
     1021 ttttatgatt gcgttatgga attcgaatca atgttattca ccgagaaatg cctccaaatt
     1081 tttgtattct gccgtatatt gattaaatgc tgcttcatct ttattgacga gtgcgtcatc
     1141 tataagttct atgagctgtc gttttcttac ctctcgaagt gcttcattga taattaattc
     1201 aatacccaaa tcattgacag ttgtgacaaa cgcttctaaa gttgtatgtt taacattagt
     1261 attgtatttc atagaaacca ctacctttct ttaatttata ataagtatac atttttctaa
     1321 ttttgaaatc aatgaatatt ctgactatta gttaaaa
//
LOCUS       DAHHYJ010000252         1351 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000252 DAHHYJ010000000
VERSION     DAHHYJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.260"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..1149)
                     /gene="scpA"
                     /locus_tag="NBK22_002646"
     CDS             complement(<1..1149)
                     /gene="scpA"
                     /locus_tag="NBK22_002646"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCD2415794.1"
                     /translation="MKRNFPKLIALSLILSLSVTPIANAESNSNIKAKDKKHVQVNVE
                     DKSVPTDVRNLAQKDYLSYVTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVL
                     NTEGNIDYIVTISPKVTKYSSSSSKYTINVSPFLSKVLNQYKDQQITILTNSKGYYVV
                     TQNHKAKLVLKTPRLEDKKLKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQ
                     YINKLENFKIRETQGNNGWCAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQRFQF
                     TGLTPREMIYFGQTQGRSPQLLNRMTTYNEVDNLTKNNKGIAVLGSRVESRNGMHAGH
                     AMAVVGNAKLDNGQEVIIIWNPWDNGFMTQDAKNNVIPVSNGDHYRWYS"
BASE COUNT          420 a          203 c          195 g          533 t
ORIGIN      
        1 tgaataccat cgataatggt cgccattgga tactggaata acattgtttt tagcatcttg
       61 agtcataaaa ccattatccc atggattcca tataattata acctcttgtc cattatctaa
      121 tttagcgttc ccaacaactg ccatggcatg ccctgcgtgc ataccatttc ttgattctac
      181 tctactacct aaaacagcaa ttcctttatt atttttagta agattgtcaa cttcattata
      241 tgtagtcatt ctattaagaa gttgtggact tcttccctga gtttgtccaa aataaatcat
      301 ctctcttggc gttaaaccag taaattggaa tcgttgtcct tgtaagtttg ggtgtaaaaa
      361 tctcatcaca gcttctgcat gatatttgtt agtattataa gtcgcattta gtaatgcaga
      421 catcgtatag cctgcacacc aaccattgtt accttgagtt tctcttatct tgaaattctc
      481 aagtttattt atatattgct cgttgtaagt ataattatta cttttaaatt gactagttgg
      541 catagtgaca gaagcttttt gctttagttg cgttacatta ttgccagtag gtatactctc
      601 ggtctttttt aactttttat cttctagacg tggtgttttt agtactagtt tagctttatg
      661 attttgagta accacatagt aaccttttga gtttgtcaaa atagtaattt gttgatcttt
      721 atattgattt aagactttag ataaaaatgg agaaacattg attgtgtatt tagaagatga
      781 actagaatac ttagtgactt ttggtgaaat tgtaactata taatcgatat taccttcagt
      841 gttcaaaact ggaaaataat aattaccatc actctttttg ttgaatttat aaattttaaa
      901 aggttcacca agtgtatacg aggctttctc tttattatag attttatcaa gtgaagtaac
      961 ataagaaaga tagtcttttt gcgctaaatt acgtacgtca gtaggaacac tcttgtcctc
     1021 gacattaact tgcacatgct tcttatcttt tgctttgata tttgaattgc tctcagcgtt
     1081 tgcaataggg gtaacactta aagaaagtat taaacttaaa gctattaatt ttggaaagtt
     1141 tcttttcata taaaaactcc tttattttat tataaaataa ttatattaaa tttagttaag
     1201 agtttcaata taaattttta atctatgttt aaaaaatatt taatttttac acattatcag
     1261 tttatgtaat aatttagtag cttatttact aaattgcaaa catctcattc aataattaat
     1321 atgatcgaaa gtttcatgaa aatatagtag t
//
LOCUS       DAHHYJ010000253         1350 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000253 DAHHYJ010000000
VERSION     DAHHYJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.261"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            536..>1350
                     /locus_tag="NBK22_002647"
     CDS             536..>1350
                     /locus_tag="NBK22_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD2415795.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQS"
BASE COUNT          519 a          201 c          170 g          460 t
ORIGIN      
        1 acaagtcgac tatggtaaca aaattcaata tcttaatttt tatcatacac aactatctaa
       61 cgcattattt gcaattaaat ttgcccatta acctattttt cataaaatgt catttaaaca
      121 agttatttat taaaattcac tttattacat aaattataca attaaaaagt ttcttcaaat
      181 tgtaaagatg cattaatcga gttataatca taatgattaa gatggtagat ttagtcttat
      241 ataactacat ttactaagat ttaaatagat gagtacataa tgtataaatt gggaatatac
      301 agtatgactt acggtaatat atcaatatat tgatatatta tttgcacatg ggagtatgct
      361 attttttatc tatacatctt aaaaatattt acagttttat aattcaatat cattaaacaa
      421 acaacatatt cagcttattt ttcattctct tacgttacaa gttgtaaata ccatcgcgtt
      481 aaacaacaat agccaacgct aataaaatat attgcgttaa agtggagtga ttaatatgtc
      541 aaacgtacta acagaaatag atagtcaata tccatatatg actaaaaatg aaaaaaagat
      601 agctaaattt atactaaatt cacctcaaaa ggtgattaaa atgaggtctc aagacttagc
      661 aagcttatta gatattagta catcatcagt cattcgattc agcaagaaaa ttactgatgg
      721 cggttttcac gacttgaaaa taaatatatc taaatatgtt cctaaagcat catcaattta
      781 caatgttgaa ttaatgaata acgaaagcac agaatcttta agaactaaac tccatacacg
      841 tactacacgc gcacttaatc atgctaataa cgaattaaat gataaaacta ttgatcaaat
      901 atgtcattgt ttaaaacgtt ctgagacgat ttttatatat ggttttggcg catcttttgt
      961 agttgctacc gacttatacc aaaagttatc aagaataggt cttaatattc aacttgttca
     1021 agaaacacat atttttgcaa cattattagc aactcacaat tcgaacgaca gtgttattct
     1081 tattactaac aatggtacgc aaagtgaaat gcaatcaatg gttaaagtta ttgatgacta
     1141 ccatatacct ataattacaa ttactagtac aagggataat cccgtagcac aggcatcaaa
     1201 tattgtttta acttatggga aaactgatga aaatgagatg catatgggag caacaacttc
     1261 attatttgct caaatgttca ctattgatat tttatattat cgttatgttg cattaaatta
     1321 tcacgcatca ttagatttca taacacaatc
//
LOCUS       DAHHYJ010000254         1323 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000254 DAHHYJ010000000
VERSION     DAHHYJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.262"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            124..1278
                     /locus_tag="NBK22_002648"
     CDS             124..1278
                     /locus_tag="NBK22_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD2415796.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRINNLDDCVNLTIGQPDFPMPD
                     VVKKAYIDAINNDKTSYSHNKGLLETREAISQYFKNRYHFSYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRNQLILIGGLSKSHSATGIRIGFLLGPQYLIDKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT          470 a          202 c          210 g          441 t
ORIGIN      
        1 gacatttttc gtttttcaaa taaagcttaa gtaatttacc cttttttgct ttaaaagtat
       61 gttatgatat tattaatttc taaattttta gacaatattt gatttatatt tgaggtgtca
      121 caaatgaaac tttctttaaa ttctaattct aaatatttaa gagcaccaag tattcgccaa
      181 ttttcaaatc gtataaataa tttggatgat tgtgttaatt taactattgg ccaaccagac
      241 ttcccgatgc ctgatgttgt gaaaaaggca tatattgatg ctattaacaa tgacaagaca
      301 tcatactctc acaataaagg gttattagaa actcgcgaag caattagtca atacttcaaa
      361 aatcgttatc atttttccta tgatcctgaa gaaattattg tgacaaatgg agcaagtgaa
      421 gcaatagaca caacgttacg ttctatcata gagcctggag atgaaattat aataccggga
      481 ccaatttatg caggctacat accactcatc gaagtactag gtggtaaacc aatttatatt
      541 gatacaacag caacacaatt caaaattaca cctgatgcgt tagaaagtca tatttctcca
      601 aagaccaaag ctgtcttgtt aaattatccg actaatccaa ctggcgtagt tttaaaaaga
      661 aatgaagttt taaatatcgt aaatgtatta aaaaaatatc cgatatttat tattagcgat
      721 gagatttatg ctgaaaatac atttagtggt aaacatgtat ccttcgctga atttgaagac
      781 attcgtaatc aattaatatt aattggtggt ttaagtaaat cacactcagc aaccggaata
      841 agaattggtt ttctattagg acctcaatat ttgattgata aattaacatt tatgcacgcc
      901 tataattgta tttgtgccaa tgttccagct caaatagcat gtataacagc acttaacgaa
      961 ggattagaag ctcctaaata tatgaacgaa gcttatgtag aacgaagaaa ttatttagta
     1021 tctgaattaa ctaaattagg ttttgagatt accgctcaac ctgaaggcgc gttttatatt
     1081 ttcccaagta ttaaacatat caccgatgac gatttcgaat tttgtgtcga tttacttgaa
     1141 tcaacacatt tagcaatcgt tccaggctca tcctttacag aattcggtaa aggatttgta
     1201 aggatttcct atgcttatga aatggatgtt ttaaaagaag gtatgaagag acttgctaaa
     1261 tatttaaata ctaaataaaa atgatatgtt gaactggacg tcgtgaacta aattcatagt
     1321 acg
//
LOCUS       DAHHYJ010000255         1304 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000255 DAHHYJ010000000
VERSION     DAHHYJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.263"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(6..>1304)
                     /locus_tag="NBK22_002649"
     CDS             complement(6..>1304)
                     /locus_tag="NBK22_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD2415797.1"
                     /translation="MFMSAIESSIISLALPTIKQDLNAGNLISLIFTAYFIALVIANP
                     IVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQGFGSGVLMSLSQI
                     VPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYINIPIAIIAII
                     LVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNFLSFILAIVVA
                     MCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIPVYLQEQLGLSPL
                     QSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIISFGIKLPVLIA
                     FVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIGSTIMGYLYAI
                     QSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE"
BASE COUNT          508 a          229 c          173 g          394 t
ORIGIN      
        1 taattctatt cttttgattg agatgactgt tctttaaaaa atacgaccca taaaacaata
       61 agcccaatgc taattacagc aacagcactt aacacattgt gtaagtttgg accaaagatt
      121 cctgattgta tcgcataaag atatcccatt attgttgaac ctattgatgc cccaagattt
      181 ttagttaatc cataaaatga catcattttc ttcatttgta atgggctagt ttcctcttgg
      241 acaatcacac tatctttcgt ataaatatat ccaaaactta atcctgccaa aattaacaca
      301 aatgctataa gtaccggcaa tttaatacca aatgaaatga taatactact tactagtagc
      361 aatgtaaatg atagtaagta aataactttc cttgatagtt ttgcttcaat tcgatgtaaa
      421 ttaaaattca atgtaatcca agctacagat aaaggaaaaa taaccaatcc actttgcaat
      481 ggagataatc ctagttgttc ttgaaggtag actggaatat ataaattgaa tcccattaaa
      541 caaatagctg ttagaaggtc agttataaaa actaaagtaa tcgaacgatt aaattcaacc
      601 acaggtaaaa atggtgaaga aacatgtttt tctactttaa ataaacacat tgccacaacg
      661 atagctaaga taaaactcaa gaaatttaaa agtaacagct gttggtttaa taaagcaaac
      721 ataatcaatc caataaatac ataaaatagc gtgagccctt tagtatcaaa ttttgattta
      781 gcaactgttt cttcctctgg aaaatgaaat gtccatataa ctaatataat ggcaatgatc
      841 gcaatcggta tatttatgta gaataaccaa tgccatgttg caaattctaa tataccacca
      901 cctagaagtg gcccaatgat actcgaaata ccccaaacac tgccaactat gcccataatt
      961 ttatatcgta acggaatttc aaatgctaac tttggaacaa tttgcgataa tgacatcaat
     1021 acacctgatc caaaaccttg tatcacgcgt gatataatta gcatagtaaa attagtactt
     1081 aaaccacaca taaaactacc tatactaaat aaaagtaatc ctgcaattgc cacataaata
     1141 attttaaatc tcgaaagtaa ttcacccact ataggattgg caattactaa tgcgataaaa
     1201 tacgctgtga aaattaatga aattaaattt cctgcattta aatcttgttt aattgtaggt
     1261 aatgcaagcg aaataataga tgattcaatc gctgacataa acat
//
LOCUS       DAHHYJ010000256         1274 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.264, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000256 DAHHYJ010000000
VERSION     DAHHYJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.264"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..522
                     /locus_tag="NBK22_002650"
     CDS             <1..522
                     /locus_tag="NBK22_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD2415798.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDADSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSSSDSDSESDSNSD
                     SESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANSSLIWGLLASLGSL
                     LLFRRKKENKDKK"
     gene            743..>1274
                     /locus_tag="NBK22_002651"
     CDS             743..>1274
                     /locus_tag="NBK22_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HCD2415799.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNRASAVVSGEKNPYVSESLKLTN
                     NKNKSRTVEEYKKSLDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFS
                     EEKKIKNGNTDNLDYLGLTHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEE
                     QLKCDLELNKLENQILM"
BASE COUNT          500 a          165 c          250 g          359 t
ORIGIN      
        1 agtgactcag attcagatag cgattcagat tcagacagtg attcagactc agatagcgat
       61 gcagactcag acagtgactc agactcagac agcgactcag actcagacag cgattccgac
      121 tcagacagtg actcggattc agatagcgat tctgactcag acagtgactc ggattccgac
      181 agtgattcag actcagacag tgactcagat tcagatagtg actcggattc agcgagtgat
      241 tcagactcag atagtgactc cgattcatca agtgattccg actcagaaag tgattcaaat
      301 agcgattccg agtcaggttc taacaataat gtagttccgc ctaattcacc taaaaatggt
      361 actaatgctt ctaataaaaa tgaggctaaa gatagtaaag aaccattacc agatacaggt
      421 tctgaagatg aagcgaattc gtcactaatt tggggattat tagcatcatt aggttcatta
      481 ctacttttca gaagaaaaaa agaaaataaa gataagaaat aagtaataat aatattaaat
      541 taatcatatg attcatgaag aagccacctt aaaaggtggc ttttttactt gaattttcca
      601 aatatattgt ttgaatataa ttaataatta attcatcaac agttaattat tttaaaaagg
      661 tagatgttat ataatttggc ttggcgaaaa aatagggtgt aaggtaggtt gttaattagg
      721 gaaaattaag gagaaaatac agttgaaaaa taaattgcta gttttatcat tgggagcatt
      781 atgtgtatca caaatttggg aaagcaatcg tgcgagtgca gtggtttctg gggagaagaa
      841 tccatatgta tctgagtctt tgaaactgac taataataaa aataaatcta gaacagtaga
      901 agagtataag aaaagcttgg atgatttaat atggtccttt ccaaacttag ataatgaaag
      961 atttgataat cctgaatata aagaagctat gaaaaaatat caacagagat ttatggctga
     1021 agatgaggct ttgaagaaat tttttagtga agagaaaaaa ataaaaaatg gaaatactga
     1081 taatttagat tatctaggat taactcatga aagatatgaa agtgtattta atactttgaa
     1141 aaaacaaagt gaggagttct taaaagaaat tgaagatata aaaaaagata accctgaatt
     1201 gaaagacttt aatgaagagg agcaattaaa gtgcgactta gaattaaaca aattagaaaa
     1261 tcagatatta atgt
//
LOCUS       DAHHYJ010000257         1264 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000257 DAHHYJ010000000
VERSION     DAHHYJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.265"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(57..968)
                     /locus_tag="NBK22_002652"
     CDS             complement(57..968)
                     /locus_tag="NBK22_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCD2415800.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
BASE COUNT          463 a          207 c          199 g          395 t
ORIGIN      
        1 tataaaaaac cgtaatttta atgggcgtgc cattaaaatt acggtttgct tttttgttat
       61 tgcttgtcac ttcttactga tcgtaacatt agcatgataa agtatgttgt cataccaaat
      121 aaataagtga taaataatgc atggaaaagt gcgattatca ggttaacatt tgtcataata
      181 gataatgcac ctgtgataac ttgtaatata acaagtataa atgcagctgt ataaccataa
      241 tgaacagtac ggttatttgg ataattttta acagcgtgaa tataagtaat cataataatc
      301 gtaaacacta taaaagccat gatacgatgc gtgagttgaa cccaatcttg ttctgaatgt
      361 ggtacaagat cgtggaatgg caatggccaa ccaccatatg ctaaacttgc atccgcatgt
      421 ctcactagtg caccagtata aacaccacaa taaatgatga ttgccattaa ccatgttaaa
      481 cgtcttaatg gctttttgat atataattcg tcagcttcat atttttgatc tatagagaaa
      541 ataatcaatg ttattaaaaa tacagatgag aaactgatta atgatatacc aaagtgcaat
      601 gctaaaacgt aatcgttttg ttgccaaata acagcagcag ctccgattaa tgcttgcaat
      661 aataagaatc caacactaat gattgataaa ggtttaattt ctttaatata gcctatatgt
      721 ttccatgcag tgataactaa ccataagacc attaataaag acaaagctga aacggctcta
      781 tgacttaact caataatcgt atcaatagga aagaattctg gaatcaacgc accatgacat
      841 agtggccaag aagaaccaca accatcagct gatccggttt tggtaactaa ggctccacca
      901 agttgtacaa atgtcatcat taacgttgct acgacaccta accattttaa attctttttg
      961 ccaaacaatt tatacacccc atcataaaaa aacgcatcac tgagaattag aattaatagc
     1021 cggatttgag atgatactat aaattgacct gattgtagga tatgaaaaga tgggatccaa
     1081 cgcaggaaat atgatttaat tttacaagta ttgtatgtgg acgacataaa aagaacttaa
     1141 tcgtaatgat ttttttattg ggataattat gtaatccacg tttactcaaa tttgaagctt
     1201 aaatattaat ctcttttaga tgcatgattc tacttattaa aattttctaa catatacatt
     1261 atag
//
LOCUS       DAHHYJ010000258         1219 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000258 DAHHYJ010000000
VERSION     DAHHYJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.266"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(18..1019)
                     /locus_tag="NBK22_002653"
     CDS             complement(18..1019)
                     /locus_tag="NBK22_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCD2415801.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
BASE COUNT          382 a          223 c          152 g          462 t
ORIGIN      
        1 taatcttctt ttttaaatta atttactctt tttataatca aaatagtcat atcatctttt
       61 ttatttgggt tttgaagctt taaaattgct tcatagataa tttgaacaat atcttgtggg
      121 tgcatatgtt tatgtttttt aatatattct aaaagttttt gtttatctat aaaggtacct
      181 tcactatttc tagcttcagt cacaccatcc gttaaaatga taattaaatc atcaaggtat
      241 attggaattt cttgttgttg atatcgtgtt tgtgaactga ttcctaacac tctacctcta
      301 actgaaattt cttcgaattc ttctttttca gcgcgataaa tatatccagg ctcatgacca
      361 gctgaactac aatacaataa atggttcatt tcttcatata aaccataaaa cattgtgacg
      421 aacatatttt gattaatatt cttttcaaca acacgattta aacgttttaa accatcactc
      481 ggtaattgtg agtgtccata agaatccatg ccaaacttta tcatactcat tgctaaagca
      541 gctggtatac cttttccaat gacatctgca acagcaaagc tcattgtgcc atcgttatgg
      601 tcaattaaat taaaataatc tccacttact ttttgtgccg ccactgaaat aacgccaatt
      661 tgaatactat caaattgtgg aatatctgtt ttaagcattg tttgttgtaa gctagaagct
      721 aagtctatct ctttatcgtg aacttgaagt ttatctacca atctttgata atctcgataa
      781 ctataaccaa agcctttaac gatttcttgt aagacatcta atgtcttgaa cacatcttca
      841 cgcgttaagt ttaacgtcaa tatatagttt ttgtgaatat cgacaatgtc ttcaggcaag
      901 acgtccttac gaatcacttc gtcagtgtat ttctcacact tttttatcaa ttctacttta
      961 tcttggcacg ttaaactttc atcaattaaa cccttataat gttgcttaaa ttcttccacg
     1021 attagttgcc tcctcacttc ccttacaaag caaatgctta agcaaggata attatgattc
     1081 acttactaac atttatttaa ttgcacttat caattttgtc gtttgcaatt ttgctgtagt
     1141 tgatactttt aagctaaaaa aaactttagg atggaatacc taaagcctaa aaaacttaag
     1201 acatcctttt ataataaaa
//
LOCUS       DAHHYJ010000259         1205 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000259 DAHHYJ010000000
VERSION     DAHHYJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.267"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            1..930
                     /gene="isdF"
                     /locus_tag="NBK22_002654"
     CDS             1..930
                     /gene="isdF"
                     /locus_tag="NBK22_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCD2415802.1"
                     /translation="MLVILIATAYISFVTGTIKLSFNDLFTKFTTGSNEAVDSIIDLR
                     LPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRALCMLFIPQLYFY
                     LPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLEILTQNPLMKIPQ
                     GLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGFNIDRYRWLTGLL
                     AVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGAWLLLVADLLGRV
                     IQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            992..>1205
                     /locus_tag="NBK22_002655"
     CDS             992..>1205
                     /locus_tag="NBK22_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB family sortase"
                     /protein_id="HCD2415803.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDI"
BASE COUNT          391 a          171 c          220 g          423 t
ORIGIN      
        1 ttgttagtca ttttaatcgc aactgcttat atttcgtttg taaccggtac aattaaattg
       61 tcatttaatg acctatttac aaaatttaca actggtagca atgaagcagt ggattcaatc
      121 attgatttgc gattgccacg tatattaatt gcattgatgg ttggcgcaat gttagcagtt
      181 tctggagcat tattacaagc agcactacaa aatcctttgg cagaggcgaa tatcattggc
      241 gtttcttcag gtgcacttat aatgagagca ctttgtatgt tgtttattcc acaattgtac
      301 ttttacttac cattattaag ttttattgga ggtttaatac catttttaat aattatattg
      361 ttgcattcta aatttagatt caatgcggta agtatgatat tagtaggtgt tgcgttattc
      421 gtattattaa atggtgtttt agaaatttta actcaaaacc ctttaatgaa aattcctcaa
      481 ggcttaacaa tgaaaatatg gagcgacgta tacatattag cagtatcagc attattggga
      541 ttaatattaa cattactatt gtcccctaaa ttgaatttac taaatttaga cgacatacaa
      601 gcgcgaagta tcggttttaa tattgatcgt tacagatggt taacaggttt attagcagta
      661 tttttagcaa gtgcaactgt tgcgattgtg ggacaactag cctttttagg tattattgtg
      721 ccacatgtgg ttagaaagct agttgggggc aattacagag tacttattcc attttctaca
      781 gttattggtg catggctatt gttagtggct gatttattag gacgagtgat acagcctcct
      841 ttagaaattc cagccaatgc tattttaatg attgtcggtg gtccaatgct aatttactta
      901 atttgtcaaa gtcaacgaaa tcgaatctaa aacggtacaa caactggttt agtgatgact
      961 gaagaaacgt cgttaataaa agaggatttt tatgagaatg aagcgatttt taactattgt
     1021 acaaatttta ttggttgtaa ttattatcat ttttggttac aaaattgttc aaacatatat
     1081 tgaagacaag caagaacgcg caaattatga gaaattacaa caaaaatttc aaatgctgat
     1141 gagcaaacat caagcacatg tgagaccaca atttgaatca cttgaaaaaa taaataaaga
     1201 cattg
//
LOCUS       DAHHYJ010000260         1201 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000260 DAHHYJ010000000
VERSION     DAHHYJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.268"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            57..1133
                     /locus_tag="NBK22_002656"
     CDS             57..1133
                     /locus_tag="NBK22_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD2415804.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKEEAEEAGVEVGNMVT
                     PYSEFEVLANDKYLTAKAFDNRYGCALAVEVLKRLKDENIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKEHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDESYKNIMW"
BASE COUNT          412 a          164 c          272 g          353 t
ORIGIN      
        1 tttctgaaag ttagtgatac aatagtttta agttgaaata caggaggatg aataacatga
       61 atcagtcagt caaattactt aaacatttaa cagatgtaaa cggcattgct ggttatgaaa
      121 tgcaagttaa agaagcaatg cgtaactata tagagcctgt cagtgatcaa attattgaag
      181 ataacttggg tggcattttt ggaaagaaaa atgctgagaa tggtcaatac tcaattatga
      241 tttctggtca tatggatgaa gttggtttta tggtaacaaa gattgataaa catggtttta
      301 tttcatttac gccagttggt ggatggtgga atcaagtcat gctatctcaa aaagtaacga
      361 ttacaacaga ttcgggcaaa gaaattagag gtatcatcgg ttctaaaccg ccacatgtct
      421 taacgcctga agaacgtaaa aagccaatgg aaatcaaaaa tatgtttata gatattggtg
      481 ttagtagcaa ggaagaagct gaagaagctg gcgttgaagt aggcaatatg gtaacgccat
      541 atagtgaatt tgaagtgctt gcaaatgata aatatttaac tgcgaaagca tttgataatc
      601 gctatggctg tgcattagct gttgaggtat taaaacgttt aaaagatgaa aatattggca
      661 ttaacttata cagtggtgcc acagtgcaag aagaagttgg tttgcgtggt gcgaaagtgg
      721 cagcgaatac aattaaacca gatttggcga tagctgtcga tgtaggtatt gcttatgata
      781 ccccaggtat gtcaggtcaa acgagcgata gtaaactagg cggtggtcca gttgtcatta
      841 tgatggatgc tacaagtatt gctcaccaag gtttgcgtaa gcatattaaa gatgtagcta
      901 aggaacataa catcgaagta caatgggata cgacaccagg tggaggtaca gatgcgggaa
      961 gtattcatgt cgcaaatgaa ggtattccaa cgatgacaat cggtgttacg ctgcgataca
     1021 tgcattctaa tgtttcagtg ctcaatgtag atgattatga aaattctatc cgtcttgtta
     1081 ctgaaattgt ccgttcattg aatgatgaaa gttataaaaa tatcatgtgg taatcaaatc
     1141 cataaataat aaagaatcct tttaatatgg taggttgtta aacaattgtc taactttaat
     1201 t
//
LOCUS       DAHHYJ010000261         1193 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000261 DAHHYJ010000000
VERSION     DAHHYJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.270"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>1193
                     /gene="vraG"
                     /locus_tag="NBK22_002657"
     CDS             <1..>1193
                     /gene="vraG"
                     /locus_tag="NBK22_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease VraG"
                     /protein_id="HCD2415805.1"
                     /translation="AILGIIIGIFGSKLLLMIVFTLLGIKEKVPIIFSLRAVFETLML
                     IGVAYFLTSAQNFILVFKQSISQMSKNNQVKETNHNKITFEEVVLGILGIVLITTGYY
                     LSLNIVQYYDSIGILMFILLSTVIGAYLFFKSSVSLVFKMVKKFRKGVISVNDVMFSS
                     SIMYRIKKNAFSLTVMAIISAITVSVLCFAAISRASLSSEIKYTAPHDVTIKDQQKAN
                     QLASELNNQKIPHFYNYKEVIHTKLYKDNLFDVKAKEPYNVTITSDKYIPNTDLKRGQ
                     ADLFVAEGSIKDLVKHKKHGKAIIGTKKHHVNIKLRKDINKIYFMTDVDLGGPTFVLN
                     DKDYQEIRKYTKAKHIVSQFGFDLKHKKDALALEKAKNKVDKSIETRSEAISSISSLT
                     GILLF"
BASE COUNT          441 a          148 c          195 g          409 t
ORIGIN      
        1 ggcaatatta ggtattatta ttggtatttt tggttcaaaa ctgttattaa tgattgtctt
       61 tacattatta ggaattaaag aaaaggttcc aattattttt agtttgaggg cggtatttga
      121 aacattaatg ttaatcggtg tcgcttattt tttaacctct gctcaaaatt ttatattagt
      181 gttcaaacaa tctatttcac agatgtcaaa gaataaccag gttaaagaaa caaatcataa
      241 taaaattaca tttgaagagg ttgttttagg catcttaggt atagtattga ttaccacagg
      301 atactatcta tctttgaaca ttgttcaata ttatgattct atcggtatac ttatgtttat
      361 tttattgtca actgtgattg gggcatactt attttttaaa agctctgttt ctctagtttt
      421 taaaatggtg aagaagttta gaaaaggtgt tataagtgta aatgatgtca tgttctcatc
      481 atctattatg tatcgtatta agaaaaatgc tttttcactt acggtcatgg caatcatttc
      541 agcgattact gtttcagttc tttgctttgc tgctataagt agagcgtcct tatcaagtga
      601 aataaaatat actgcaccac acgacgttac aattaaagac caacaaaaag ctaatcagtt
      661 agcaagtgaa ttaaacaatc aaaaaattcc tcatttttat aattataaag aagtaattca
      721 tacgaaattg tataaagata atttatttga tgtaaaagcg aaagaaccat acaatgtaac
      781 aattactagt gataaataca tccctaatac tgatttgaaa cgtgggcaag ctgatttatt
      841 tgtagcggaa ggttctatca aagatttagt gaaacataag aagcatggta aagcaattat
      901 aggaacgaaa aaacatcatg ttaatattaa gttacgtaaa gatattaata aaatctattt
      961 tatgacagat gttgatttag gtggaccaac gtttgtctta aatgacaaag actatcaaga
     1021 aataagaaag tatacaaagg caaagcatat cgtctctcaa tttggattcg atttgaaaca
     1081 taaaaaagat gctttagcat tagaaaaagc gaaaaataaa gttgataaat ctattgaaac
     1141 aagaagtgaa gcgataagct caatatcaag tttaaccgga atattattat ttg
//
LOCUS       DAHHYJ010000262         1189 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000262 DAHHYJ010000000
VERSION     DAHHYJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.271"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            89..1057
                     /locus_tag="NBK22_002658"
     CDS             89..1057
                     /locus_tag="NBK22_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCD2415806.1"
                     /translation="MLLNLLLIFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFVTFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQASATKIIVSGGQGPDEPISEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
BASE COUNT          370 a          195 c          163 g          461 t
ORIGIN      
        1 agacttacat gttgctgcgt tctccttgct ttaataaatt aaatcatcta catttatatt
       61 taatatttat tataagcgag gtgtcagtat gttattgaat ttattattaa tatttatact
      121 tatcgttatt tgtccgttaa ttttcaacta tactctgacg ttaaatttaa catgctacat
      181 cttagttgtc atatcgatat ttttgtcact catctatagc atcatgacct atactttccc
      241 ttggtctatg tttgtaacat ttataataat cagtattatc atgctattca aacatagata
      301 tttattttct catacttcta gtcgcttatt tattaaaaaa ttattatctt tttcatataa
      361 atttgtactc tacacatcag cacttctatt tataagtacg attcagtcac ctattgtgaa
      421 aggtttgtct atgtggttag ctatattatg tctttccctg ttactaacct ttatttgtta
      481 tctcgtttgg acagtatcat atagacatag atcatacaac aaagaagttg atatcataat
      541 tgtgttaggt gctggtattt ttaccgaatt tgtcacacca atgcttgcag cgcgtttaga
      601 ccgtgcactt gatatctatc aacaacaagc atcagcaaca aagattattg tatctggcgg
      661 acaaggacca gacgaaccaa tttctgaagc tttagctatg caacgttatt tgattgcaca
      721 tggtgttgat aaaacagata tactaatgga atctcactct ataaatacat acacaaactt
      781 tttatattct aaacaaatca tcaacaattt gtatcatgaa gcagttaaaa ttgtttgtgt
      841 aacaagtcag tttcatgtat tgcgtgcctt acgactagcc caaaaattag gtttatctat
      901 cgatggtgtc ggaagtcata caccatatca ctttttctta catgtgctca ttaaagatta
      961 tctaggcgta atgtatcagt atagattgtt attaacatta tattgttcaa gtagttggtt
     1021 tatatgtctt tacgccgctt ttatttataa tagttaatcc acaaacaaac tattattata
     1081 ggttgtttct aatattgcgt caaactatct ttaaatattc gttaatcctt gattagacaa
     1141 ttgttatatt gaaaataaat aaactaaaga gtctgggaca ttaatccct
//
LOCUS       DAHHYJ010000263         1169 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.272, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000263 DAHHYJ010000000
VERSION     DAHHYJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.272"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..918
                     /locus_tag="NBK22_002659"
     CDS             <1..918
                     /locus_tag="NBK22_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulase"
                     /protein_id="HCD2415807.1"
                     /translation="LDLIMGYKDEERANKKAVNKRMLENKKEDLETIIDEFFSDIDKT
                     RPNNIPVLEDEKQEDKNHKNMAQLKSDTEAAKSDESKRSKRSKRSLNTHNHKSASQEV
                     TAEQKAEYERKAEERKEKFLAKNKDNPVVSLIDDEDDNENDKQLVVSAPSKKPTTPPT
                     YTETTTQVPMPTFERQTQQQIVYKAPKQLAGLNGESHDFTTTHQPPTTSNHTHNNVVE
                     FEETSALPGRKSGSLVGISHIDSSHLTEREKRVIKREHVREAQKLVDNYKDTHSYKDR
                     LNAQQKVNTLSEGHQKRFNKQINKVYNGK"
BASE COUNT          515 a          176 c          181 g          297 t
ORIGIN      
        1 ttagatttaa ttatgggata taaagatgaa gaaagagcaa ataaaaaagc agttaacaaa
       61 aggatgttag aaaataaaaa agaagactta gaaaccataa ttgatgaatt ttttagtgat
      121 atagataaaa caagacctaa taatattcct gttttagaag atgaaaaaca agaagataaa
      181 aatcataaaa atatggctca attaaaatct gacactgaag cagcaaaaag tgatgaatca
      241 aaaagaagca agagaagtaa aagaagttta aatactcaca atcataaatc tgcatctcaa
      301 gaagtaactg cagaacaaaa agcagaatac gaaagaaaag ctgaagaaag aaaagaaaag
      361 tttttagcta agaataaaga taatccagtg gtttcactaa tcgatgacga agacgacaac
      421 gaaaacgaca agcaacttgt ggtttctgcg ccatcaaaga aaccaacaac accgcctaca
      481 tacactgaaa caacgacaca ggtaccaatg cctacatttg agcgtcaaac tcagcaacaa
      541 atcgtttata aagcaccaaa acaattggct ggattaaatg gcgaaagtca tgatttcaca
      601 acaacgcatc aaccaccaac aacttcaaat cacacgcata ataatgttgt tgaatttgaa
      661 gaaacgtctg ctttacctgg tagaaaatca ggatcactgg ttggtataag tcatattgat
      721 tcttctcatc taactgaacg tgagaagcgt gtaattaagc gtgaacacgt tagagaagct
      781 caaaagttag ttgataatta taaagataca catagttata aagaccgatt aaatgcacaa
      841 caaaaagtaa atactttaag tgaaggtcat caaaaacgtt ttaataaaca aatcaataaa
      901 gtatataatg gcaaataatt aatgcatggc tgcaaagcaa ataatgagtt tgccgtaaaa
      961 ataacatcat tttaatctag caataaataa tatcaaagtc atcatttcaa tgatgcaatc
     1021 tagtatagtc cacattctaa acaggtgtgg actattactt ttttcacttt atattacgaa
     1081 aaaattatta tgcttaacta tcaatatcaa taattaattt taagctgaaa aacaataaaa
     1141 atgttaagac aacgtttatt tcaagttaa
//
LOCUS       DAHHYJ010000264         1166 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000264 DAHHYJ010000000
VERSION     DAHHYJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.273"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>1166
                     /locus_tag="NBK22_002660"
     rRNA            <1..>1166
                     /locus_tag="NBK22_002660"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          267 c          358 g          248 t
ORIGIN      
        1 gatttcacac tgccgagaaa agcctctaga tagaaaatag gtgcccgtac cgcaaaccga
       61 cacaggtagt caagatgaga attctaaggt gagcgagcga actctcgtta aggaactcgg
      121 caaaatgacc ccgtaacttc gggagaaggg gtgctcttta gggttaacgc ccagaagagc
      181 cgcagtgaat aggcccaagc gactgtttat caaaaacaca ggtctctgct aaaccgtaag
      241 gtgatgtata ggggctgacg cctgcccggt gctggaaggt taagaggagt ggttagcttc
      301 tgcgaagcta cgaatcgaag ccccagtaaa cggcggccgt aactataacg gtcctaaggt
      361 agcgaaattc cttgtcgggt aagttccgac ccgcacgaaa ggcgtaacga tttgggcact
      421 gtctcaacga gagactcggt gaaatcatag tacctgtgaa gatgcaggtt acccgcgaca
      481 ggacggaaag accccgtgga gctttactgt agcctgatat tgaaattcgg cacagcttgt
      541 acaggatagg taggagcctt tgaaacgtga gcgctagctt acgtggaggc gctggtggga
      601 tactacccta gctgtgttgg ctttctaacc cgcaccactt atcgtggtgg gagacagtgt
      661 caggcgggca gtttgactgg ggcggtcgcc tcctaaaagg taacggaggc gctcaaaggt
      721 tccctcagaa tggttggaaa tcattcatag agtgtaaagg cataagggag cttgactgcg
      781 agacctacaa gtcgagcagg gtcgaaagac ggacttagtg atccggtggt tccgcatgga
      841 agggccatcg ctcaacggat aaaagctacc ccggggataa caggcttatc tcccccaaga
      901 gttcacatcg acggggaggt ttggcacctc gatgtcggct catcgcatcc tggggctgta
      961 gtcggtccca agggttgggc tgttcgccca ttaaagcggt acgcgagctg ggttcagaac
     1021 gtcgtgagac agttcggtcc ctatccgtcg tgggcgtagg aaatttgaga ggagctgtcc
     1081 ttagtacgag aggaccggga tggacatacc tctggtgtac cagttgtcgt gccaacggca
     1141 tagctgggta gctatgtgtg gacggg
//
LOCUS       DAHHYJ010000265         1118 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000265 DAHHYJ010000000
VERSION     DAHHYJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.274"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(42..1052)
                     /locus_tag="NBK22_002661"
     CDS             complement(42..1052)
                     /locus_tag="NBK22_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCD2415808.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSDNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSP
                     YTSLENEGQSVFSPFKRLILYGIILIAMTAFLNLMSTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISVIIVYVLSTNFVCKWTNPIINLQRPSQFK
                     DS"
BASE COUNT          429 a          144 c          160 g          385 t
ORIGIN      
        1 gaatgaagtg gttacagtta atgtacttag acattataaa attatgagtc tttaaactgt
       61 gatggtcttt gtaaattaat tattggattt gtccatttac aaacaaagtt tgtagataat
      121 acatatacga tgattacaga tatacttatt aaataaagat acgtcattaa tgatatagga
      181 ttatcgaatg ggtaccattc aaatcctcta acaatgccaa taattaaacc atgtaataaa
      241 tatacgtata gcgtacgact accaatataa gtatataatt ttttctttgt tgacattaaa
      301 tttagaaacg cagtcattgc gattaatata attccatata atataagtcg tttaaaagga
      361 ctgaatacac tctgtccttc attttcaagt gaagtatatg gtgaacttcc taataaccaa
      421 tctgcattga taggatgaat cacgtaaatg ataaaaaaca aaataaaggt aatgatagat
      481 actggtatta gttttttatt tttaaaaata gccgtatgtt ttttggtgaa aatgtaacct
      541 aggtaaaata ttgggaaaaa tacgattgtc cttgaaatgc tcaagtagct atcgatgtta
      601 tctgaaaaac ctgctccaat agatataata attgaaactg atagcacttt atatggatta
      661 aatcttctaa ctattactaa aatgacatga aagaaaaata gcgtgatcaa aaaccataac
      721 gcaaatactg ggttaaaagg atcaagttgt aattcgtcac ttttacctgt taagaaataa
      781 tatattgaaa agaatgcaaa aaatatcata taaggtacta tcaaacgttt tgaaattttt
      841 tctaaatagt atggtttatc aatatttttc gcgaaataac cagatataaa caaaaatgtt
      901 ggcatatgga aactataaat aactaggtat aatgctgata aatatttatc gccactagtg
      961 tagggttgta acatatgtcc aaatacgact aataatatta aaattgctct tgcgttgtca
     1021 aaaaaataat ctctctcttt taatgaagtc atattttatc tcctttatgt ccataagact
     1081 cttactaatt tattatattt tgtattagtt atgcaaca
//
LOCUS       DAHHYJ010000266         1046 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.275, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000266 DAHHYJ010000000
VERSION     DAHHYJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.275"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            118..>1046
                     /gene="hyl"
                     /locus_tag="NBK22_002662"
     CDS             118..>1046
                     /gene="hyl"
                     /locus_tag="NBK22_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCD2415809.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     TVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYK"
BASE COUNT          399 a          160 c          183 g          304 t
ORIGIN      
        1 taaatatact aatataaacg attataaata tttgatatgt ctcaactgca atattctaaa
       61 ttgacatatt gattattgtt tcctcaaatc gtttaaaaaa atagaaggat gatgaaaatg
      121 aaaacacgta tagtcagctc agtaacaaca acactattgc taggttccat attaatgaat
      181 cctgtcgcta atgccgcaga ttctgatatt aatattaaaa ccggtactac agatattgga
      241 agcaatacta cagtaaaaac aggtgattta gtcacttatg ataaagaaaa tggcatgcac
      301 aaaaaagtat tttatagttt tatcgatgat aaaaatcaca ataaaaaact gctagttatt
      361 agaacgaaag gtaccattgc tggtcaatat agagtttata gcgaagaagg tgctaacaaa
      421 agtggtttag cctggccttc agcctttaag gtacagttgc aactacctga taatgaagta
      481 gctcaaatat ctgattacta tccaagaaat tcgattgata caaaagagta tatgagtact
      541 ttaacttatg gattcaacgg taatgttact ggtgatgata caggaaaaat tggcggcctt
      601 attggtgcaa atgtttcgat tggtcataca ctgaaatatg ttcaacctga tttcaaaaca
      661 attttagaga gcccaactga taaaaaagta ggctggaaag tgatatttaa caatatggtg
      721 aatcaaaatt ggggaccata tgatagagat tcttggaacc cggtatatgg caatcaactt
      781 ttcatgaaaa ctagaaatgg ctctatgaaa gcagcagata acttccttga tcctaacaaa
      841 gcaagttctc tattatcttc agggttttca ccagacttcg ctacagttat tactatggat
      901 agaaaagcat ccaaacaaca aacaaatata gatgtaatat acgaacgagt tcgtgatgac
      961 taccaattgc attggacttc aacaaattgg aaaggtacca atactaaaga taaatggata
     1021 gatcgttctt cagaaagata taaaat
//
LOCUS       DAHHYJ010000267         1034 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000267 DAHHYJ010000000
VERSION     DAHHYJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.276"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            5..80
                     /locus_tag="NBK22_002663"
     tRNA            5..80
                     /locus_tag="NBK22_002663"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Val,seq:tac)
     gene            97..172
                     /locus_tag="NBK22_002664"
     tRNA            97..172
                     /locus_tag="NBK22_002664"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:130..132,aa:Thr,seq:tgt)
     gene            178..253
                     /locus_tag="NBK22_002665"
     tRNA            178..253
                     /locus_tag="NBK22_002665"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Lys,seq:ttt)
     gene            257..338
                     /locus_tag="NBK22_002666"
     tRNA            257..338
                     /locus_tag="NBK22_002666"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:291..293,aa:Leu,seq:tag)
     gene            342..416
                     /locus_tag="NBK22_002667"
     tRNA            342..416
                     /locus_tag="NBK22_002667"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:374..376,aa:Gly,seq:gcc)
     gene            427..515
                     /locus_tag="NBK22_002668"
     tRNA            427..515
                     /locus_tag="NBK22_002668"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:461..463,aa:Leu,seq:taa)
     gene            534..607
                     /locus_tag="NBK22_002669"
     tRNA            534..607
                     /locus_tag="NBK22_002669"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:568..570,aa:Arg,seq:acg)
     gene            617..690
                     /locus_tag="NBK22_002670"
     tRNA            617..690
                     /locus_tag="NBK22_002670"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:651..653,aa:Pro,seq:tgg)
     gene            708..783
                     /locus_tag="NBK22_002671"
     tRNA            708..783
                     /locus_tag="NBK22_002671"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:741..743,aa:Ala,seq:tgc)
     gene            805..878
                     /locus_tag="NBK22_002672"
     tRNA            805..878
                     /locus_tag="NBK22_002672"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:839..841,aa:Met,seq:cat)
     gene            907..980
                     /locus_tag="NBK22_002673"
     tRNA            907..980
                     /locus_tag="NBK22_002673"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:941..943,aa:Ile,seq:cat)
BASE COUNT          234 a          243 c          285 g          272 t
ORIGIN      
        1 aatcggagaa ttagctcagc tgggagagca tctgccttac aagcagaggg tcggcggttc
       61 gaacccgtca ttctccacca tttattctta catattgccg gcctagctca attggtagag
      121 caactgactt gtaatcagta ggttgggggt tcaagtcctc tggccggcac catggaagag
      181 ccattagctc agttggtaga gcatctgact tttaatcaga gggtcagagg ttcgaatcct
      241 ctatggctca ccatttgcgg gtgtggcgga attggcagac gcactagact taggatctag
      301 cgccttacgg cgtgggggtt cgactccctt cacccgcata tgcagaagta gttcagcggt
      361 agaatacaac cttgccaagg ttggggtcgc gggttcgaat cccgtcttct gctccatttt
      421 tatagtgccg gggtggcgga actggcagac gcacaggact taaaatcctg cggtgagtga
      481 tcaccgtacc ggttcgattc cggtcctcgg caccattttc aataaaaaca tatgcgcccg
      541 tagctcaatt ggatagagcg tttgactacg gatcaagagg ttatgggttc gactcctatc
      601 gggcgcgtta attatacggg aagtagctca gcttggtaga gcacttggtt tgggaccaag
      661 gggtcgcagg ttcgaatcct gtcttcccga tatactgtaa ttattatggg ggcttagctc
      721 agctgggaga gcgcctgctt tgcacgcagg aggtcagcgg ttcgatcccg ctagtctcca
      781 ccatattatt tacaaactat ataaggcggt gtagctcagc tggctagagc gtacggttca
      841 tacccgtgag gtcgggggtt cgatcccctc caccgccact atttattagt tgtaaaatta
      901 tatttaggac ctttagctca gttggttaga gctaacggct cataaccgtt cggtcgcagg
      961 ttcgagtcct gcaaggtcca tataattttg gaggaatacc caagtccggc tgaagggatc
     1021 ggtcttgaaa accg
//
LOCUS       DAHHYJ010000268         1031 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000268 DAHHYJ010000000
VERSION     DAHHYJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.277"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..256
                     /locus_tag="NBK22_002674"
     CDS             <1..256
                     /locus_tag="NBK22_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCD2415810.1"
                     /translation="NMYQYLVPSDLGLKNSQVVQFPISNISSKKILHNKFKSIVNTEE
                     IDENISFYKLVNKFSPEVAPEDTVDLLQIIKRRSKDGNFK"
     gene            240..818
                     /locus_tag="NBK22_002675"
     CDS             240..818
                     /locus_tag="NBK22_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HCD2415811.1"
                     /translation="MEILNDSVKSFEKLYLQLQDGFPVIVPTDTNYNLCSLPNNDLCI
                     DKIFEYKKRSKDKPLSLFIDKPEDWKLYGDNQNTEIVDKLVEIFWPGPLNIILKNKTM
                     RRFISYINSPIAITSANISGTADDILITENEAIKHMGENVRYMLRSQNKTNYKTSSTI
                     IKVTDNKIELLREGDIKFEEIKERLGTGIIYE"
     gene            811..>1031
                     /locus_tag="NBK22_002676"
     CDS             811..>1031
                     /locus_tag="NBK22_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415812.1"
                     /translation="MNKLILGIYLYRIFSRAYFYLPFLLIYFLIQGYSIIQLEILMAS
                     YGIAAFLFSLYKEKCFKICNLKDSNKLVV"
BASE COUNT          409 a          111 c          155 g          356 t
ORIGIN      
        1 aaatatgtat caatatttag ttcctagtga tttaggattg aaaaattcac aagttgttca
       61 gtttccaata agtaatattt ctagtaagaa aatcttgcac aataaattta aatcaatagt
      121 taacactgaa gaaattgatg aaaatatttc tttctataaa cttgtgaaca agttttcacc
      181 tgaagtagca cctgaagata cagtggattt acttcagata ataaaaagga ggagtaaaga
      241 tggaaatttt aaatgattct gtaaaaagtt ttgaaaaact ttatttacaa ttacaagatg
      301 gttttccagt gattgttcct actgatacta attataattt atgtagttta cctaacaacg
      361 atctttgtat cgataaaata tttgaatata aaaagcgatc gaaagataag ccattatcat
      421 tatttattga taagccagag gattggaaat tgtatggaga taatcaaaat acggaaatag
      481 ttgataaact agttgaaata ttttggcctg gaccattaaa tattatttta aaaaataaaa
      541 cgatgagaag atttatttca tatattaatt cacctatagc aattacttca gcaaatatat
      601 ctggaactgc cgatgatatt ttaataactg aaaatgaagc aattaagcac atgggcgaaa
      661 atgtaagata tatgctaaga agtcaaaata aaactaacta taaaacatct agtacgatta
      721 ttaaagtgac agataataaa attgaattat taagagaagg agatataaag tttgaagaaa
      781 taaaagaaag actaggtaca ggtattattt atgaataagt taatacttgg gatttattta
      841 taccgaattt tttcacgagc atacttttat ttaccgtttt tattaattta ctttttgatt
      901 caaggttatt ccataataca attagaaata ttaatggcgt cttatggcat tgcagcattt
      961 ttattctctc tatacaaaga gaagtgtttt aaaatttgta acttaaaaga ttctaataaa
     1021 ttagttgtta g
//
LOCUS       DAHHYJ010000269         1020 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000269 DAHHYJ010000000
VERSION     DAHHYJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.278"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            20..544
                     /locus_tag="NBK22_002677"
     CDS             20..544
                     /locus_tag="NBK22_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCD2415813.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRILRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      531..628
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            703..>1020
                     /locus_tag="NBK22_002678"
     CDS             703..>1020
                     /locus_tag="NBK22_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCD2415814.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLV
                     IYKL"
BASE COUNT          373 a          117 c          196 g          334 t
ORIGIN      
        1 ttgaaagaag ggaaaacgca tgaatcatga caaatcacca ataattttaa ttggtttcat
       61 gggtaccggt aaatctacga ttggtaaata cgttgcagat gagcaaaatt tatcatttat
      121 tgatatagat tcatatatcg aagagaagta taagttaaca ataccagaaa tatttagtaa
      181 acatggtgaa caatatttca ggaatttaga gttcacatgt ttgcaagaat gtattaacac
      241 tgcagatata attgctactg gtggtggtat tattgaaagt gaagaggcat ttaatttttt
      301 gaaaaatcaa aaaaacatta tttggttaga ttgtaatatt gatattatat atagtcgaat
      361 caatgatgac ccacatcgac ctaatgcaaa taataagaca atcaagcagt taaatgattt
      421 gtattgctcg cggattttaa gatataatga aatcgcattc aagaaatttg atagtcattt
      481 gctatcaatt tcagaaatat attatgaatt gctaaattta ataaaagcga gtgatcagta
      541 ttagagagaa tagagcgtta agactctatc gccgaaggtg caagtaattt attacgaaac
      601 tctcaggcaa aaggataata ctgtaacgcg ttcctgaatt ggtgatttat aaacagggta
      661 gcgattgcta tcctgttttt ataattttaa gggggtattt caatgtcaag tgatttaaaa
      721 caaacacctt tatatcaaaa ttatgttgat agaggtgcaa aaattgtgga attcggagga
      781 tgggcgatgc ctgttcaatt ttcaagtatt aaagaggagc ataatgctgt tcgatacgaa
      841 attggcctgt ttgatgttag tcatatgggt gaaattgaag taacaggtaa agatgctagt
      901 cagtttgtgc aatatttatt atcaaatgat actgataatt taactacttc aaaagcatta
      961 tatactgctt tatgtaatga agaaggcggt attattgatg atttagtaat atataaatta
//
LOCUS       DAHHYJ010000270         1011 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.279, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000270 DAHHYJ010000000
VERSION     DAHHYJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.279"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(39..440)
                     /gene="msa"
                     /locus_tag="NBK22_002679"
     CDS             complement(39..440)
                     /gene="msa"
                     /locus_tag="NBK22_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCD2415815.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFKTNIDKTYIFFNIIFIDFYYYIYNVHLMTLPKFNNYIKAEMMELEDIDVLITSKD
                     FGFDEILFYTLYLLLILIVLYYLKKQVKHKI"
     gene            complement(637..837)
                     /gene="cspA"
                     /locus_tag="NBK22_002680"
     CDS             complement(637..837)
                     /gene="cspA"
                     /locus_tag="NBK22_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCD2415816.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
BASE COUNT          389 a          164 c          108 g          350 t
ORIGIN      
        1 atcatataca taccacactt taaacaagca tcatgaaatt agattttatg ctttacttgc
       61 tttttcaaat aatataaaac gataagaatt aacaacaaat ataatgtata aaacagaatt
      121 tcatcaaatc caaaatcttt acttgtaatt aatacatcaa tatcttctaa ttccatcatc
      181 tctgctttaa tataattatt aaactttggc aaagtcatta aatgtacatt gtaaatatag
      241 taataaaagt cgataaatat gatgttaaaa aatatgtatg ttttatcaat attagttttg
      301 aaaaatagta tcccagaaat cattatcgga atcactaata caatcatcat tgctgctaaa
      361 atattaatat ttaatccaat tgcagataat acaccgaaaa ctaataaatt agcaactaaa
      421 ctaaggataa gatatttcat aaaatcacct cgtcgttgta caccataatt atattatata
      481 aatatatttt tctattgcaa acaatgttgg tgatataaaa aaagagttct caatttggtt
      541 gagaaactca tcgcgttaag tatttgtaat aagcatataa aaacacctta cttcttggta
      601 aggtgttagt taaaatcaat gattcaaatc taagaattat agtttaacaa cgtttgcagc
      661 ttgtggaccg cggtcgcctt caactacttc aaactcaaca gcttgacctt cttctaatga
      721 tttgtaacca tcttggttaa ttgctgaaaa atgtacgaat acgtcatttt ctccttcaac
      781 ttcgataaag ccgaatcctt tttcagcgtt aaaccattta actgtacctt gtttcataat
      841 ctgaaacctc caagactaaa attcattcaa tatgcttatt cgcatattac aaaaaataca
      901 tgcaaatttc actcaaatat tctttgcaat atggaataat catctgctta acttgtatta
      961 tagtgcagtt attaccaaat tgcaatacaa taataaaaaa tattcattta a
//
LOCUS       DAHHYJ010000271         1007 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.280, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000271 DAHHYJ010000000
VERSION     DAHHYJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.280"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(137..634)
                     /locus_tag="NBK22_002681"
     CDS             complement(137..634)
                     /locus_tag="NBK22_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HCD2415817.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKVFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDILEDFKGVDRNDIELRQQRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
                     KTIYV"
     gene            complement(552..851)
                     /locus_tag="NBK22_002682"
     CDS             complement(552..851)
                     /locus_tag="NBK22_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCD2415818.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            complement(838..>1007)
                     /locus_tag="NBK22_002683"
     CDS             complement(838..>1007)
                     /locus_tag="NBK22_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCD2415819.1"
                     /translation="KLKVGKIINVAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFH
                     IEKGRIRYEKL"
BASE COUNT          335 a          153 c          108 g          411 t
ORIGIN      
        1 aaaaagtgaa aatggaaaaa taaattgctg ttagcatcaa taataacggc gtaacatatc
       61 ctttttcttt atataaatca aaattaataa attatttcac atccttttta atagtactca
      121 tttaaatact agtaagttat acataaatag ttttagtctg cacatttgta ccgactttaa
      181 ctgttatcgt tattttaata atggatttat agtagatatt tgcagtaaat gaagtaacat
      241 tattaatcat tgttatattt cctctgtcat ttacaacttt aataatttta ttatttatta
      301 atttgtattc gatcatttct tcacctttat gtaaaatgat acgctgttgc ctaagttcaa
      361 tatcgtttct atctactcct ttaaaatcct ctagaatatc tcttgagaaa aattcgaaat
      421 ctacagttgt taattcccta ctttcaatta gaaaagtttt gctaagtcta attaagtctg
      481 gaacaattag taaagttata cttataacca tcatcgctac taacatttca atgagcgaaa
      541 aaactttgac attaatactg tatacatgtc ttttgataag aagtggtatt ttttgaaata
      601 gcacaaattt gttggtcact ttgcttaata tcatacttcc ctatagttac ccccttctta
      661 agttcttctt tattaatttt agatacagtc gtcaaaatta ctttagaagc atcaattgtt
      721 tgtagtttat ggtttatact cgcttgcatt tgattcatca ttggtatcaa tagtaatgta
      781 atcaatccaa ttagcaaaaa tccagccata ctatctatta agaatgatcc tttacactta
      841 tagcttttca taacgaattc ttcctttttc aatatggaat attattctat aaattgaatt
      901 gttattgtca attgttatac taccaaattt attgatattc ccttttttat caaaggcaat
      961 aatatcaact tttgcaacat taattatttt gcctactttt aatttta
//
LOCUS       DAHHYJ010000272          999 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.281, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000272 DAHHYJ010000000
VERSION     DAHHYJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.281"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            352..753
                     /locus_tag="NBK22_002684"
     CDS             352..753
                     /locus_tag="NBK22_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCD2415820.1"
                     /translation="MKKNITKTIIASTVIAAGLLTQTNDAKAFFSYEWKGLEIAKNLA
                     DQAKKDDERIDKLMKESDKNLTPYKAETVNDLYLIVKKLSQGDVKKAVVRIKDGGPRD
                     YYTFDLTRPLEENRKNIKVVKNGEIDSIYWD"
     gene            713..805
                     /locus_tag="NBK22_002685"
                     /pseudo
     CDS             713..805
                     /locus_tag="NBK22_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          380 a          150 c          138 g          331 t
ORIGIN      
        1 taaacattaa ttatcaaaaa agctatatgt ataacaattt ggaatgtttg tctacccaac
       61 attgcaaaca aaatgcaaag aaatatatat cgttttaaca ataaaaatta tccaatcata
      121 gttgagtgac gtttactact attattatac tatcaacaaa ttcaataata cctcaaataa
      181 aaataatacc accaaattca tcattattct ttttcgattg cattaaacct tattaatttg
      241 cacttaattt taattaatga agtgttaact ggtaaatgca tgaaaccgtt tgtaaaatag
      301 cagtgtactc gcattagtaa gtacaaattt attttacagg gagaacttat tatgaaaaaa
      361 aatatcacaa aaactattat cgcatctaca gtaatcgctg caggtctttt aactcaaact
      421 aatgatgcta aagctttctt tagttatgaa tggaaaggtt tagaaatcgc aaaaaattta
      481 gcagatcaag ctaaaaagga cgatgaacgt attgataagt taatgaaaga gtctgataaa
      541 aatctaactc cttacaaagc tgaaactgtt aatgatctgt accttattgt taaaaaatta
      601 agccaaggtg atgtgaagaa agcagttgtc agaattaaag atggtggtcc tagagattac
      661 tatacttttg acttaactcg tcctttagaa gagaacagaa aaaatattaa agttgttaaa
      721 aacggtgaaa ttgactcgat ttattgggat taattaacat actgaattaa atagttgtac
      781 gccaaacgtt agaaaacaat gctaacgtat tggcgtgctt tttttatttt aagtaacttc
      841 caatttattt agcatcttta cgactgttta ataaagcacg tatgattaac acggttgcca
      901 ctatatccgt tacaattttt ataattgtta atacattcga tttccttttc acaatagcca
      961 cctcgcttgt tcaaaacata aacaactatt gcattcact
//
LOCUS       DAHHYJ010000273          982 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.282, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000273 DAHHYJ010000000
VERSION     DAHHYJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.282"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            93..866
                     /locus_tag="NBK22_002686"
     CDS             93..866
                     /locus_tag="NBK22_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2415821.1"
                     /translation="MIHSKRLRLWLYLVLLAVFIGACGMKKEESSKDKQIKENFNKTL
                     SLYPTKNLEDFYDKEGFRDQEFEKGDKGTWIIHSKMTIETNGKNMESRGLVLYVDRNT
                     RTTKGEFIVRELWEDKKGYSRSKEKEYPVKMEHNKIIPTKPIADDKLRKEIENFKFFV
                     QYGDFKDINDYKDGDISYNPNVPSYSAKYQLSNDDYNVKQLRKRYDIPTKKAPKLLIK
                     GDGDLKGSSIGHKNLEFTFIENKEENIYFTDSINFKPTE"
BASE COUNT          412 a          112 c          184 g          274 t
ORIGIN      
        1 cgaggattta atcataaaat ttcctctagt attattaaaa ttggaatttg aacttaactt
       61 tgcatcaaaa ataaaaatgg ggatgtgaaa tgatgattca ctcaaaaagg ttgaggctct
      121 ggctgtattt agtattatta gctgttttta taggggcttg cggaatgaaa aaggaagaaa
      181 gcagtaaaga taaacaaatt aaagaaaact tcaacaaaac gttgagcctg tatccaacta
      241 aaaatcttga agacttttat gataaggaag gctttcgaga tcaagagttt gaaaagggcg
      301 acaaaggcac ttggataatt cactctaaaa tgacaataga aacaaatgga aagaatatgg
      361 aatccagagg cttggtcctg tatgtagacc gcaataccag aacgacaaaa ggtgaattta
      421 ttgtgaggga attatgggaa gataaaaaag gatactcacg tagtaaagaa aaagaatatc
      481 cagtaaaaat ggaacataat aaaattatcc caaccaaacc gatagctgat gataaattaa
      541 gaaaagaaat cgaaaacttt aaattctttg tacaatacgg tgactttaaa gatattaatg
      601 attataaaga tggtgacatt tcatataatc cgaatgtgcc aagttattca gcaaagtatc
      661 aattaagtaa tgatgactac aatgtgaagc aacttagaaa gaggtatgat ataccaacta
      721 agaaagcacc taaattatta ataaaaggcg atggtgattt aaaaggatca tctataggac
      781 acaaaaattt agagtttaca tttatagaaa ataaagaaga aaacatctat tttacggata
      841 gtattaattt caaacctact gaatagggtt aatagaagtt tgaaatgaaa atttcataaa
      901 gtgttcaaaa taatttttcg ctttagttgt gtaaattaaa gattgagtca gtattaaaaa
      961 tataaggtgg agaaatgatg at
//
LOCUS       DAHHYJ010000274          963 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000274 DAHHYJ010000000
VERSION     DAHHYJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.283"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(90..173)
                     /locus_tag="NBK22_002687"
     tRNA            complement(90..173)
                     /locus_tag="NBK22_002687"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Leu,seq:caa)
     gene            complement(212..286)
                     /locus_tag="NBK22_002688"
     tRNA            complement(212..286)
                     /locus_tag="NBK22_002688"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Gly,seq:tcc)
     gene            complement(390..464)
                     /locus_tag="NBK22_002689"
     tRNA            complement(390..464)
                     /locus_tag="NBK22_002689"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(429..431),aa:Gly,seq:tcc)
     gene            complement(472..545)
                     /locus_tag="NBK22_002690"
     tRNA            complement(472..545)
                     /locus_tag="NBK22_002690"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(511..513),aa:Cys,seq:gca)
     gene            complement(551..622)
                     /locus_tag="NBK22_002691"
     tRNA            complement(551..622)
                     /locus_tag="NBK22_002691"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(588..590),aa:Gln,seq:ttg)
     gene            complement(634..706)
                     /locus_tag="NBK22_002692"
     tRNA            complement(634..706)
                     /locus_tag="NBK22_002692"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(671..673),aa:His,seq:gtg)
     gene            complement(709..782)
                     /locus_tag="NBK22_002693"
     tRNA            complement(709..782)
                     /locus_tag="NBK22_002693"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Trp,seq:cca)
     gene            complement(798..878)
                     /locus_tag="NBK22_002694"
     tRNA            complement(798..878)
                     /locus_tag="NBK22_002694"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(842..844),aa:Tyr,seq:gta)
     gene            complement(884..959)
                     /locus_tag="NBK22_002695"
     tRNA            complement(884..959)
                     /locus_tag="NBK22_002695"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Thr,seq:tgt)
BASE COUNT          281 a          224 c          222 g          236 t
ORIGIN      
        1 ttttatttgt attgtataga gagaaataaa aagaaacctt gttttacaag gtttctaata
       61 cgttatgtta tgtaaataac agttaattat accggtggtc ggggtcgaac cgacactcca
      121 caagtggaac gggattttga gtcccgcgcg tctgccaatt ccgccacacc ggcttaatgg
      181 taaacaaaaa acttcccttt ggaagcaatt atggagcgga agataggatt tacacctata
      241 cctcgttccg ggaaggaacg tgttctaaaa gttgaactac tcccgcaaat attaaattat
      301 ggagcggaag ataggattta cacctatacc tcattccagg aaggaatgta ttctaagagt
      361 tgaaatactc ccgcattatt attaaattat ggagcggaag ataggatttg cacctatacc
      421 tcgttccggg aaggaacgtg ttctaaaagt tgaactactc ccgcataaac ctggaggcgg
      481 caaccggatt tgaaccggtg ataaaggttt tgcagacctc tgccttacca cttggctatg
      541 ccgccaataa ctgggctagc tggattcgaa ccaacgagtg acggagtcaa agtccgttgc
      601 cttaccgctt ggctatagcc cattaataat aagggcggct gaaggggatc gaaccctcga
      661 atgtcggaac cacaatccga tgtgttaacc acttcaccac agccgccatg gcaggggcag
      721 taggaatcga acccacacca aaggttttgg agacctctat tctaccgttg aactatgccc
      781 ctattaaaaa taataaatgg aggggggcag attcgaactg ccgaacccga aggagcggat
      841 ttacagtccg ccgcgtttag ccacttcgct acccctccat aaatggtgcc ggccagagga
      901 cttgaacccc caacctactg attacaagtc agttgctcta ccaattgagc taggccggct
      961 aag
//
LOCUS       DAHHYJ010000275          825 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000275 DAHHYJ010000000
VERSION     DAHHYJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.284"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            80..649
                     /gene="agrB"
                     /locus_tag="NBK22_002696"
     CDS             80..649
                     /gene="agrB"
                     /locus_tag="NBK22_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCD2415822.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNF
                     HINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
     gene            646..786
                     /locus_tag="NBK22_002697"
     CDS             646..786
                     /locus_tag="NBK22_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCD2415823.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
BASE COUNT          289 a          106 c          119 g          311 t
ORIGIN      
        1 ttatttccta cagttaggca atataatgat aaaagattgt actaaatcgt ataatgacag
       61 tgaggagagt ggtgtaaaat tgaattattt tgataataaa attgaccagt ttgccacgta
      121 tcttcaaaag agaaataact tagatcatat tcaatttttg caagtacgat tagggatgca
      181 ggtcttagct aaaaatatag gtaaattaat tgttatgtat actattgcct atattttaaa
      241 catttttctg tttacgttaa ttacgaattt aacattttat ttaataagaa gacatgcaca
      301 tggtgcacat gcaccttctt ctttttggtg ttatgtagaa agtattatac tatttatact
      361 tttaccttta gtaatagtaa attttcatat taacttttta attatgatta ttttaacagt
      421 tatttcttta ggtgtaatct cagtatatgc tcctgcagca actaaaaaga agcccattcc
      481 tgtgcgactt attaaacgaa aaaaatatta tgcgattatt gttagtttaa cccttttcat
      541 tatcacactt atcatcaaag agccatttgc ccaattcatt caattaggca tcataataga
      601 agctattaca ttattaccta ttttctttat taaggaggac ttaaaatgaa tacattattt
      661 aacttatttt ttgattttat tactgggatt ttaaaaaaca ttggtaacat cgcagcttat
      721 agtacttgtg acttcataat ggatgaagtt gaagtaccaa aagaattaac acaattacac
      781 gaataattta aatagagagt gtgatagtag gtggaattat taaat
//
LOCUS       DAHHYJ010000276          795 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.285, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000276 DAHHYJ010000000
VERSION     DAHHYJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.285"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            237..422
                     /locus_tag="NBK22_002698"
     CDS             237..422
                     /locus_tag="NBK22_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415824.1"
                     /translation="MNKHINKKATKLLEDYKKVQQRKSEETDRGCLKSVIMLIVLFFL
                     IIFGIVACSTNVNFFFQ"
BASE COUNT          266 a          126 c          117 g          286 t
ORIGIN      
        1 gcaccattac tattcattta tgtattctag tgcttcatta atattttaat gcatcagttt
       61 aaggaaattc catttggaaa ataaaaagca aatatcatca aattaacata tttaacgtta
      121 tactttcata aaatttgaaa caattgaaca tccttacttt agctttatat ctttatttca
      181 agtcatgttt tcaactattt taatgaatta aacatatatt ataaggagtc aacatcatga
      241 ataaacatat aaataaaaaa gcgacaaaac tacttgaaga ttataaaaag gttcaacaaa
      301 gaaaatcaga agaaactgat agaggttgtt tgaaaagtgt catcatgctt atcgtcttat
      361 ttttcttaat tatttttggt atcgtggcat gttctacaaa cgtaaacttt ttcttccagt
      421 aaaagagtga gatagaaatg ataaagaacc actaatgatt tattatgtag tggttcttta
      481 tcattagctg tagctaatgt ctacttaaaa aatgaataca tgagtaaagc tcatgcataa
      541 gaaatactaa ttgctaaaga aaaagtattt ctttatcgat gtcctacccc aacttgcaca
      601 tttttgtaag ctgacttttc gccagcttct atgttggggc cccgccaact tgcattgttt
      661 gttggaattg gtgatcctat ttctctctgt tggggcccca ccccaacttg cattgtttgt
      721 agaatttctt ttcgaaattc tctgtgttgg ggccccacga gtattgaaaa aacttgttat
      781 aagcttattt tcatt
//
LOCUS       DAHHYJ010000277          785 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000277 DAHHYJ010000000
VERSION     DAHHYJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.286"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            235..636
                     /locus_tag="NBK22_002699"
     CDS             235..636
                     /locus_tag="NBK22_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCD2415825.1"
                     /translation="MTSYEEETDYRNVPRELLDNNIPMGRGMIKWAPFATLPEQFETI
                     QQYIIDQNKITRPVLSDDQLAELNIRLHEALQYAQPVEVKFYNNGFVDSVRLTIYRID
                     AINYEIDGYVYNQQQRQKISIFDILDIVLLP"
     gene            complement(654..785)
                     /locus_tag="NBK22_002700"
                     /pseudo
     CDS             complement(654..785)
                     /locus_tag="NBK22_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          268 a          124 c          119 g          274 t
ORIGIN      
        1 ttattaaatt ttagctagaa ttaacaatca tttatcattt ccttaaagct aaaactattt
       61 aactacttta aaagaactat ttgctgtgat atgtcattat tcgattttaa ttaatccctc
      121 ttttttactt acacgagcat taattattaa cgcgtttatg cttccacaaa atttttgttg
      181 caaaatagaa cacatgttcg tataatgtaa ttatcagatt ggggtgaatt aagggtgaca
      241 agttatgaag aagaaactga ttatcgtaac gttccaagag aattactaga taacaatata
      301 cctatgggaa gaggcatgat taaatgggct ccttttgcaa cattacccga acaattcgaa
      361 acgattcaac aatacattat cgaccaaaac aaaattacac gtcctgtatt atcagatgat
      421 caattggctg agctaaacat tcggctacac gaggcgctac aatatgccca acctgtagaa
      481 gttaaatttt ataataatgg ctttgtagat tcagtacgct taaccattta tcgtattgat
      541 gctattaatt atgaaattga tggttatgtt tacaatcagc aacaacgtca aaaaatttct
      601 atctttgata tcttagatat tgtcttgtta ccttaatgct ttgaacataa ttaaacagat
      661 tgagtatatg catataagat gatttttaac atcatttttg gatgatagga tgttgcgcca
      721 cgatgatgtc tgaattcatc gaattcgcta tcaggtattg tttcaacaat ttcattaaca
      781 tatcg
//
LOCUS       DAHHYJ010000278          778 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.287, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000278 DAHHYJ010000000
VERSION     DAHHYJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.287"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..549
                     /locus_tag="NBK22_002701"
     CDS             <1..549
                     /locus_tag="NBK22_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415826.1"
                     /translation="LSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVKTML
                     QFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSA
                     LANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAI
                     ILLYALGSILAVYCISHFKRAE"
     gene            553..>778
                     /locus_tag="NBK22_002702"
     CDS             553..>778
                     /locus_tag="NBK22_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD2415827.1"
                     /translation="MKFLKDTSIAEISSILYLIFPIAGIFFNEVYGPKWLYIISVIVF
                     SLSYLILVIVNNRLNTLMFYILLIIHYFIIC"
BASE COUNT          241 a           91 c          123 g          323 t
ORIGIN      
        1 ttaagtttta gtttactaac ttttccatta gatattatta atgaaaaaca aaatgaatgg
       61 cgccaaagat taatggtaac accatttact tttactagtt attatatttc aaaagtagtg
      121 aaaactatgc tgcaatttgc aatagcgata ttagttattt ttatggttgg acatttttat
      181 aaaggtgttg caatgagtgc agttcaatgg ttagagtcag gaatattttt atggttaggt
      241 gcgtctctat taataacttt tggcatatta ttttctttgt taaatgatat tcaaaaaaca
      301 agtgctttag ctaatatcgt aacaattggt ttagcagtat taggtggatt gtggtttccg
      361 ataaacacat ttccaaattg gcttcaacat gttgctcatg ttttaccgag ctatcatttg
      421 cgtaaactag gtgtagatat tgcttcaaat catcatatca atttaatatc atttgctata
      481 atactcttgt atgctttagg gagtatatta gcagtatatt gtattagtca ttttaaaagg
      541 gcggaataaa atatgaaatt tttaaaagat acttcaattg ctgaaatatc gtctatactt
      601 tatctgattt ttcctattgc cggtatattt tttaatgaag tatatggtcc caaatggttg
      661 tatattatat cagtcattgt cttttcgttg tcgtatctta tattagttat agtaaataat
      721 agacttaata cattaatgtt ttacattttg ttgattattc attattttat tatttgtt
//
LOCUS       DAHHYJ010000279          776 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.288, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000279 DAHHYJ010000000
VERSION     DAHHYJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.288"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            130..>776
                     /locus_tag="NBK22_002703"
     CDS             130..>776
                     /locus_tag="NBK22_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCD2415828.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSKFDSKR
                     LSNAKMSFINPTQLENKNTNDRLLKHDLLFHDMFVNDDWKKDFKVEFENEALSKKFIN
                     KDIDIFAGNYGYGCHGGATNKTQCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQAEIEL
                     TAVKTKKKIVTIQELDVQLRNYLNEKYKLYEQGGDIVKGYVKYHNDDEQNIEYNF"
BASE COUNT          293 a           90 c          143 g          250 t
ORIGIN      
        1 gcttgttaca agcgcatgtt cgttcagtca actactgcca atataacttc gtagagcata
       61 gaacattgat ttatttccca gcctgtttat catttatgtc tcgctctcaa atgtaaatta
      121 ataattgttt tgggggagtt tgaagttaaa tatttaacag gatttatttt aatattattg
      181 ttagaaggaa tttttacaaa ttcagcgagt gcaatcgaat attcagactt acatcataaa
      241 agtaagtttg attcaaagcg tctaagtaat gctaagatgt cattcatcaa tccaactcag
      301 cttgaaaata aaaacacaaa cgatagactg ttgaagcatg atttgttatt tcatgacatg
      361 ttcgtaaatg atgattggaa aaaggatttt aaagttgaat ttgaaaatga ggcactttca
      421 aagaaattta taaataagga tatcgatata tttgctggaa attatggata cggatgtcat
      481 gggggagcaa ccaataaaac gcaatgtagt tatggtggtg ttactttaag tgacaataat
      541 aagtacgatg attataagaa tataccttgt aatttatgga ttgacggaca tcaagcagaa
      601 atagaattaa cagcagtaaa aacgaaaaag aaaattgtta ccattcaaga attagatgtt
      661 caattaagaa attatttgaa tgagaagtat aagttgtacg aacaaggtgg cgacattgtt
      721 aaagggtatg ttaaatatca taatgatgat gaacaaaata tagaatataa ttttta
//
LOCUS       DAHHYJ010000280          764 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000280 DAHHYJ010000000
VERSION     DAHHYJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.289"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..753
                     /locus_tag="NBK22_002704"
     CDS             <1..753
                     /locus_tag="NBK22_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD2415829.1"
                     /translation="EQIEVEDNGINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQ
                     LIQWAQEKEERFIIEDDYDSEFRYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRV
                     AYMVLPYSIMKKYHSQNHIEGNTVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTY
                     ILKRLKPYKEQLDIQGAETGMHFTITVKNGLTLQECLERANKVKLKLQVYNFDDDQDY
                     KKTPKFILGFGGIDDDALKPHVDALIKSLIIK"
BASE COUNT          299 a          110 c          123 g          232 t
ORIGIN      
        1 gaacaaattg aagttgaaga taatggaata aacgtagatg aagttattaa atcacaaaaa
       61 aatatagtat atataacgcc ttcccatcaa tttccaactg gatatgtgat ggacttaaaa
      121 aaacgaactc aattaataca atgggctcaa gaaaaagaag aacgatttat tatagaagat
      181 gattatgatt ccgagtttag atatttcggc aaacccattc cagcaattca aggtctatat
      241 tcaagaggag aaaaagtgat ttatatcagt acattttcaa agtctatttt tcctagctgt
      301 cgtgttgcat atatggtgtt accctattct ataatgaaaa aatatcattc tcaaaatcac
      361 attgaaggca acactgtacc tgttcatatg caaaacctta ttgcaacgtt tatatcttcc
      421 ggtggttttg aaaggcattt aaataaaatg agacgaattt atcgtagaaa actaacctat
      481 attttaaaaa gattgaaacc ttacaaagag cagcttgata ttcaaggtgc tgaaacgggt
      541 atgcatttta caattacagt aaaaaatgga ttaacattac aagagtgtct tgaaagagca
      601 aacaaagtaa agttaaaatt acaagtttat aattttgatg atgatcaaga ctataagaaa
      661 acccctaaat ttatattagg ctttggcggt atagacgatg atgctttaaa accgcatgta
      721 gacgctctaa taaaatcatt aattataaag taacacttct ttga
//
LOCUS       DAHHYJ010000281          752 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.290, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000281 DAHHYJ010000000
VERSION     DAHHYJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.290"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..335
                     /locus_tag="NBK22_002705"
     CDS             <1..335
                     /locus_tag="NBK22_002705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415830.1"
                     /translation="YVEKLKKDIEIINQLKKEYENSEEKKESDFNNIVNEAIEFRKTL
                     LENLEIPKDANEKIDECYEELRKEMLTKLKEYHYFENQLYPDLDKQIAYILSKYNDQS
                     SKKYISDL"
     gene            complement(546..>752)
                     /locus_tag="NBK22_002706"
     CDS             complement(546..>752)
                     /locus_tag="NBK22_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4888 domain-containing protein"
                     /protein_id="HCD2415831.1"
                     /translation="NERIDANNTWKNLTNKLKGQKIIKDGDKVTIHSKDQKDPKISGI
                     VGQDFTEHKNYMLYKKDIDKITIN"
BASE COUNT          297 a           83 c          109 g          263 t
ORIGIN      
        1 aatatgtaga aaagttaaaa aaagacattg aaataatcaa tcaattaaaa aaggaatatg
       61 aaaatagtga agaaaaaaaa gagagtgatt ttaataatat agtaaatgaa gctatagaat
      121 ttagaaaaac actattagaa aatcttgaaa ttcctaaaga tgcgaatgaa aaaattgatg
      181 agtgctatga agaactcaga aaagaaatgt taactaagct aaaagaatat cattattttg
      241 aaaatcaact ttatccagat ttagataaac aaattgcgta tatattgtct aaatacaatg
      301 accaatcaag taaaaaatac atttctgatt tgtaaatttg gtgaaaatat agaatttaac
      361 tatgcgacta cgagagtggt ggttttttac gctgagaaac gccctgtgtt gcagtaagcg
      421 atgagtgtgt atatagaaat agataaagta tagatataga tttagaaatt ataaattaat
      481 atttgagata ctgcctcttt aaaaaaagac aagttacatt aaaagtaacc tgtctttatc
      541 tataactagt ttatagttat tttatctatg tctttcttgt ataacatata attcttatgc
      601 tcagtaaaat cttgtccaac tattccagag attttaggat ctttttggtc tttactatga
      661 atggttactt tatcaccatc tttaataatt ttctgtcctt ttaatttgtt agttaaattc
      721 ttccatgtat tattagcatc aattctttcg tt
//
LOCUS       DAHHYJ010000282          751 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000282 DAHHYJ010000000
VERSION     DAHHYJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.291"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            146..595
                     /locus_tag="NBK22_002707"
     CDS             146..595
                     /locus_tag="NBK22_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002475708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5085 family protein"
                     /protein_id="HCD2415832.1"
                     /translation="MELDALVMPNLAKMEFSINPEYWKDEMSRIKSFFIDKRIYTVGP
                     IVFTREVVGLDEMKFVTYISLNSEIDDIPELNIKFIPSIALLPTISHKCFDEDEFENV
                     YMKIKEFAVENNITLSNSPFYHVMVDYFGGQVYEIHAEINLDEDELE"
     gene            592..>751
                     /locus_tag="NBK22_002708"
     CDS             592..>751
                     /locus_tag="NBK22_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415833.1"
                     /translation="MNELSYSTIMFRNVISKEYLDIDLKNLNEVTTHFLNEVKHYDLN
                     ENGPLIITI"
BASE COUNT          295 a           86 c          116 g          254 t
ORIGIN      
        1 attaaacaca gcagatgatg actaaatgat atagatttac aaaacacact tttaaaaagt
       61 gaattgatct gaaaaagtga tttgaaaatc ggaaattcta tagagttttt cactgctttt
      121 atattttaaa ttggaggaat aataaatgga acttgatgca ttagtaatgc ctaacttagc
      181 taaaatggaa ttttctatta atccagaata ttggaaagac gaaatgagca gaattaaatc
      241 attttttata gacaaacgta tctatacagt tggaccaata gtatttacac gagaagtcgt
      301 cgggctagat gaaatgaagt ttgtaaccta tatttcatta aattctgaaa ttgacgatat
      361 acctgaattg aatattaaat ttataccttc aattgccctt ttaccaacta tttctcataa
      421 atgctttgat gaagatgaat ttgaaaatgt ttatatgaaa atcaaagagt ttgcagtaga
      481 aaataacata acgctatcaa attctccatt ctaccatgtg atggttgatt attttggtgg
      541 tcaagtatat gaaattcacg ctgaaatcaa tttagatgag gatgaattgg aatgaatgaa
      601 ttaagctatt ctactataat gtttagaaat gttatatcaa aagaatattt agatatagac
      661 ttaaaaaatt taaatgaagt gacaacacat tttttaaatg aagtaaagca ttatgattta
      721 aatgaaaatg gaccattaat tatcactatt a
//
LOCUS       DAHHYJ010000283          743 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000283 DAHHYJ010000000
VERSION     DAHHYJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.292"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..253)
                     /locus_tag="NBK22_002709"
     CDS             complement(<1..253)
                     /locus_tag="NBK22_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCD2415834.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTYQEK"
BASE COUNT          255 a          116 c           93 g          279 t
ORIGIN      
        1 ctttttcttg ataagtaaat aaatctaaat gtctaaaact caagacttca tcaattttca
       61 tttgtgttac acccaattcc tttaattctt ttttatggat atcaacacaa taatgacaac
      121 cgttaatttt tgaaattaat aacttaatca atgcactaag ttcatcatta acgggtgatt
      181 gatgaataaa cttttcacct tggctcatta cagacattaa ctctccattt tgtttcttgt
      241 aattataagg catttgcaat ctctccttta cattgtttaa taccaatcca gtgatactac
      301 acttcgaaca atattggata gaatttgaaa ttctgtataa catcttgtca atctatgctt
      361 tagttaaaac atttagcgct tatatttaaa aaatatataa aatatgaaaa cagattgaaa
      421 ggatattata ccttgctcta ccctttttta taaaaaatca cacacatttg agaaaatttg
      481 ataatgtcaa taaaccacgt catcgacatt gttattcgat atatattgtt cttataatgg
      541 ccaaactatt attccattta aattaacgtg gttcgtttgt gttaatgtac tgtacaaaac
      601 tggggaagta ttggtgatga tgaagtgatt tatattaatt gcgaaacacc ttcgatagca
      661 atgacataga ttattggaag attaaatcaa aaataaagaa gaccctagtg accttctttt
      721 ttgtctaatt cttctttgat ttt
//
LOCUS       DAHHYJ010000284          731 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.293, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000284 DAHHYJ010000000
VERSION     DAHHYJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.293"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            123..>731
                     /locus_tag="NBK22_002710"
     CDS             123..>731
                     /locus_tag="NBK22_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD2415835.1"
                     /translation="MYKNYNLIQLTLPIETSVRIPQIDISRYVNEIVETIPDSEFDKF
                     RHHRGATSYHPKMMLKITLYAYTQSVFSGRRIEKLLHDSIRMMWLAQNQTPSYKTINR
                     IRVNPNTDALIESLFIQFHSQCLKQNLIDGTKVEANANRYTFVWKKSIQNHESKLNEN
                     SKTLYRDLVEEKIIPEIKEDGDSDLTIEEIDLIGSHLDKEIEG"
BASE COUNT          287 a          104 c          110 g          230 t
ORIGIN      
        1 aaaaaataag acactttgcc aaacttgcac ataaatgttt aattcaataa tttgaatttt
       61 ctgtgttggg tcccttcgta taatttaata aataccacta aactaaatta atgaagtgcc
      121 ttatgtataa aaattataac ttgatccaac ttacactacc aatagaaact tctgttagaa
      181 ttcctcaaat tgatatttcg cgatatgtta atgaaattgt tgaaacgata cctgatagcg
      241 aattcgataa attcagacat catcgtggcg caacatccta tcatccaaaa atgatgttaa
      301 aaatcacctt atatgcatat actcaatctg tattttctgg tcgtagaata gagaaattac
      361 ttcatgacag tattcgaatg atgtggttag ctcaaaatca aacaccttct tataaaacta
      421 ttaatcgtat tagagtgaat cctaatactg atgcgttaat tgaatcttta tttatccagt
      481 ttcatagtca atgtttaaag caaaatctta ttgatggtac aaaagtagaa gctaatgcca
      541 ataggtatac atttgtgtgg aagaaaagta ttcaaaatca cgaatcgaaa ttgaacgaaa
      601 attcaaaaac attatatcgt gacttagttg aagaaaaaat aataccagag ataaaagaag
      661 atggagatag cgatttaaca atagaagaaa tagatttaat tggtagtcat ttagataaag
      721 aaatcgaagg t
//
LOCUS       DAHHYJ010000285          731 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.294, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000285 DAHHYJ010000000
VERSION     DAHHYJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.294"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(326..559)
                     /locus_tag="NBK22_002711"
     CDS             complement(326..559)
                     /locus_tag="NBK22_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415836.1"
                     /translation="MVFNDWEINVVYSGEHEVVTNEKSLFVIDEFYDVAIAIYYLDGK
                     LKVAHVNYGSDFTIDVANKVFELNIQNPNVDEH"
BASE COUNT          258 a          106 c           73 g          294 t
ORIGIN      
        1 tttagtttta atataaacta ttaggataat cgacgtaaga agaatcatat atattaatga
       61 tgaagaagtc catacaaaat ccgcatcatt tgttgttaat aatgggacta taattaatcc
      121 gaaattaatc atgcatgcta tatatactat aatgttatac acaatgttaa tttttgttca
      181 ccaccttata cttctatttt aaaaacttct ttataatgat atattgttta atgttgaaat
      241 aattagatta tctaattttc atttgcttta catgtaaaat gctatatata gtatgctctt
      301 tatgattcta aatgcttttt aatatttaat gctcatcaac atttggattt tgaatattca
      361 attcaaaaac tttattagct acgtcaattg taaaatcaga accatagttg acatgagcta
      421 cttttaattt tccatctaaa taatagattg cgattgcaac atcgtaaaat tcgtcaatga
      481 caaataaact cttttcgttt gttacaacct catgctctcc tgagtataca acgttaattt
      541 cccaatcatt aaaaaccatt tgttaacctc cttgaacatt taaattgatt caacttaagt
      601 ttaacttatt catacaactt cgtacaatat ctagatgaac attaattgta tttctagaaa
      661 tctttttcaa ttatatgtac taattatact tttaaatttc ttatttcagt atagttttaa
      721 aacgatttta a
//
LOCUS       DAHHYJ010000286          721 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.295, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000286 DAHHYJ010000000
VERSION     DAHHYJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.295"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
BASE COUNT          298 a           97 c           83 g          243 t
ORIGIN      
        1 taatttttta acatcgtttt gttgttttga attataaaaa ctaaagtaaa aatgttggat
       61 gtgaaagatt gttgatgaga attttgagat gtagataaat atcaaactag tctataggta
      121 aaaatttata attaatttga aaagaatgtc tcaaataatg tttgaattag caggtgtcat
      181 actaagtaat gttaagtaaa cattttatac tttatcagaa caaaaaagac atgcatcaac
      241 atgtcagtga aactaatcca tattatctta acaacgaaat tgaatcgaac ctgaaaaacg
      301 tatttacaaa tcgcaactac tctatccaag gcgaaatcgc aaaatctgat ccccgaaata
      361 acatatctcg aaaaaaattc cataattcaa tcatcttcaa taattaacaa tacattacta
      421 gttccaaata tactcataca tacatgtttc attgattgac tgcatattgt cataaataat
      481 aggggaacta atcgctaata atattgttaa acacaataca taataaagtg aaagatttta
      541 aatacatgaa ttgttcacca ttccgccata tattttcaat tacctaattt ccgaataaat
      601 attacaattt taattcaata attaatattt aagaccaaaa taatcctaaa aagtcaattt
      661 taattgcgtg atattgagtt ttaagcatat ttaaataaat gagtcaatta atgtataatt
      721 a
//
LOCUS       DAHHYJ010000287          703 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000287 DAHHYJ010000000
VERSION     DAHHYJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.296"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            109..>703
                     /locus_tag="NBK22_002712"
     CDS             109..>703
                     /locus_tag="NBK22_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD2415837.1"
                     /translation="MVCEVIAIYKNPKYRIIKYNDEYLMVNIINNWLVLFIPLLNWLT
                     PKRYIKISQEELESLNTFKPAKNNAFWPALGSSVLFSVTFRKYMPLFNVRLEKTIVIA
                     IFFVVFLGILFFYLNLNRRLALSVFTMNKEKSQKMILLPNLKHFFFIIFAYLYFGGFS
                     FFTLYALITIDVQNIIIFICWGAVTMFFFFINIVPIGI"
BASE COUNT          250 a           74 c          102 g          277 t
ORIGIN      
        1 caaataacaa tgaataaaat ttttaatgat agtaagttga taatatttta atgtcttttg
       61 aagtatcgaa aaatgtatta cacataaaat taaagggatg tggagacatt ggtttgcgag
      121 gtaatagcta tatacaaaaa tcccaaatat agaattatta aatataatga tgaatatttg
      181 atggtgaata taataaataa ttggttagtt ttattcattc cattgcttaa ttggttgaca
      241 ccaaaaagat acatcaagat cagtcaagaa gaattagaaa gtttaaatac tttcaaacct
      301 gctaaaaaca atgcattttg gccagcatta ggtagttcag ttttatttag tgtgactttt
      361 agaaagtata tgcctttatt taatgttaga ttggaaaaaa caatagtaat agccatattt
      421 tttgttgtat ttcttggtat tttatttttc tacctaaatt taaatcgtag gctagcatta
      481 agtgttttta ctatgaataa agaaaaaagt cagaaaatga tattgctacc taatttaaaa
      541 cattttttct ttataatatt tgcatattta tatttcggtg gtttttcatt tttcacttta
      601 tatgccctga taactataga tgttcaaaat ataattatat ttatctgttg gggtgcagta
      661 actatgtttt tcttttttat aaacattgtt cccattggca tta
//
LOCUS       DAHHYJ010000288          699 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000288 DAHHYJ010000000
VERSION     DAHHYJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.297"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(128..414)
                     /locus_tag="NBK22_002713"
                     /pseudo
     CDS             complement(128..414)
                     /locus_tag="NBK22_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(381..509)
                     /locus_tag="NBK22_002714"
     CDS             complement(381..509)
                     /locus_tag="NBK22_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415838.1"
                     /translation="MKVIFYCPKRADDILKASMEYQSTDYKRRYVSWRKMKVILLT"
BASE COUNT          190 a          126 c           91 g          292 t
ORIGIN      
        1 ttgtttggat agatttattt ccaataaaca tctttaaacc attcaatgaa aatcctgatt
       61 gtccaggatg gttatgtaac ataacaattg aattcggttt actgttaaat aataattcgg
      121 ttgcttgtta ccctggcaca aaagatacac tatcttgatc gccatatact tttgtaactt
      181 tagcaccttt taataaataa gctacttcat tttagctaac ttctcagctt cttgaaccgc
      241 atctttaata cgttcatctg attttttgcg ttctctagct atacgctctt taatcaattg
      301 tgatacttct tcttgagaaa atgttttctc attctgttgt gattgttcat cactgttgtc
      361 tagttgctct tcgttctgag ttacgtcagt aatattactt tcatttttct ccatgagaca
      421 tacctccgtt tatagtctgt cgactgatat tccatacttg cttttaaaat gtcatcagca
      481 cgttttggac aataaaaaat aaccttcaca ttggaaggtt aaaaaagtgt ataaaaatag
      541 caccactttc tctttatctg tgtagaaagg atgctatttt tctaattttt gttctttatt
      601 cttattttct aattctattg ctttcttgta ttctttatcc aataattctt gtgactgttt
      661 attagttatt tcaccatcta actgtgcaaa atacatttt
//
LOCUS       DAHHYJ010000289          671 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.298, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000289 DAHHYJ010000000
VERSION     DAHHYJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.298"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(230..454)
                     /locus_tag="NBK22_002715"
     CDS             complement(230..454)
                     /locus_tag="NBK22_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415839.1"
                     /translation="MLIQERGLKMTELNNIINSLQSLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKLEDARFRTIIKLYSYYVSLKEH"
     gene            complement(411..557)
                     /locus_tag="NBK22_002716"
     CDS             complement(411..557)
                     /locus_tag="NBK22_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415840.1"
                     /translation="MKLFYIVFLIIIWLNIFLGNEIIHTLTVLITTLYIVNSRKGIKN
                     DRVE"
BASE COUNT          215 a          114 c           82 g          260 t
ORIGIN      
        1 gtgttggggc ccatccccaa cttgcacatt attgtaagct gacttttcgt cagcttctgt
       61 gttggggccc cgcctataat tgaaaaatgc ttgttacatg ggcattttca ttcggtcaac
      121 tactaccaat ataatattgt agagcctaag acattgattt attatgtctt aggctctatt
      181 ccttcattta atgattcaat tattatagca atactttatt gtcccatgat tagtgttctt
      241 ttaatgagac atagtaacta taaagtttaa taatcgttct aaatctagca tcttctagtt
      301 tggttttccc atttcttaaa tcttgtactg tttgatatgg aactcctgaa tttttcgaaa
      361 ttttatagcc tgattcagac tcaaacaaag attgaagaga gtttataata ttattcaact
      421 ctgtcatttt taatcccctt tcttgaatta acaatataca atgttgttat taaaacagtc
      481 agtgtatgga tgatttcatt acctaaaaat atattcagcc atataataat aagaaatacg
      541 atataaaata gtttcatttt aatcccctat catattttta ttaatcaaat ttcaataaga
      601 ttcataatta aaagctaacc tatactcaaa attttcttat aatgcctcta actaaaaacc
      661 tacattttat t
//
LOCUS       DAHHYJ010000290          656 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000290 DAHHYJ010000000
VERSION     DAHHYJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.299"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..465
                     /locus_tag="NBK22_002717"
     CDS             <1..465
                     /locus_tag="NBK22_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCD2415841.1"
                     /translation="STQKYKKIYARFLVIINVLTTITVVFALCNLYYFAAVQNHYDLF
                     NYWLMGTISIIISYFLLVIGGMFTLLKLPKVTKCWGGKTKTHFGLLLTALSSFIYIEK
                     IIEYILVPNVVESKFIIIVSMLVIAGAQFVAFQFIMQYSRFYIFELNTEDDD"
     gene            570..656
                     /locus_tag="NBK22_002718"
                     /pseudo
     CDS             570..656
                     /locus_tag="NBK22_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5080 family protein"
BASE COUNT          221 a           68 c          103 g          264 t
ORIGIN      
        1 agtacacaga aatataaaaa aatatatgcc cgatttttag ttattataaa tgtcttaact
       61 actatcactg tagtatttgc actgtgtaac ctttattatt ttgcagctgt acaaaatcat
      121 tatgatttat ttaattattg gttgatgggt acgatttcaa tcataattag ctatttttta
      181 ttagtaattg gcggaatgtt tacgttgtta aaattaccta aagtaacaaa atgctggggt
      241 ggtaaaacta aaacacattt cggtttatta ttaactgcgt tgagttcatt tatatatatt
      301 gaaaaaatta tcgaatatat attggttcct aatgtcgtag aatccaagtt tataattatt
      361 gtgagcatgt tggttatcgc tggagcacaa tttgtggcat ttcaatttat tatgcaatac
      421 agtagattct atatttttga attaaacact gaagatgatg actaaataat tttataaatt
      481 actgttggag tatacttata taaaaaatca acttcaacac attatttttt actcaatgat
      541 gtgaaagaat ttgagtggat tgatattaat gggatttgat tattagaatt agaaaaggaa
      601 cgtttgcttt tgttttaata aatctaatat ttttcagttg ttggttattt attctg
//
LOCUS       DAHHYJ010000291          645 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.300, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000291 DAHHYJ010000000
VERSION     DAHHYJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.300"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..394)
                     /gene="lspA"
                     /locus_tag="NBK22_002719"
     CDS             complement(<1..394)
                     /gene="lspA"
                     /locus_tag="NBK22_002719"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCD2415842.1"
                     /translation="MYKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPI"
BASE COUNT          262 a           98 c           72 g          213 t
ORIGIN      
        1 atattggaaa atcataacca aaaatatttg tatcaataaa gtcaacaact tctcctgtta
       61 aaattctatc aataaagttt ccaagtgcac ctgcaaaaag taaactaata gcaacttgca
      121 taaacaaatt atattgagca tctttaataa agaaatatac taaggctatt aatataataa
      181 tggtaataat aaagaaaaat gtcatttttc cactcaatat tccccatgca gcaccattat
      241 ttcgatgtga tgttatgttt aaaaagtgcg gtatcacttc aaatgaatct ccaattttca
      301 ttgtagtagc tataatatat ttagtaactt ggtcaaatat aacgacaaat actgctatta
      361 aaatggaagt gccaataaaa tattttttgt acattttcgt tcctccaatc aatcgtccat
      421 gagacaactc tttatattat agcttacacc tgctaataaa aaaagtaagc atattacatt
      481 aaatctaatg ttactaactc aatacttgat aaacaactat gttttgacat taaatatgaa
      541 cttaattatt catttatcat atttaagatg acattaaaaa ttaggaaagc aggctggaac
      601 ataaatccct aaaaagacag tagtaagata ttttctaatt aaaaa
//
LOCUS       DAHHYJ010000292          642 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.301, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000292 DAHHYJ010000000
VERSION     DAHHYJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.301"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(229..579)
                     /locus_tag="NBK22_002720"
     CDS             complement(229..579)
                     /locus_tag="NBK22_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCD2415843.1"
                     /translation="MKFKKYILTGTLALLLSSTAIATIEGNKADASSKKDYIIQSEFH
                     DKRIAEELKSLLDQSYVNDLAAGSLNPYYKRTIMMNQYRAKAALKSNNFAKMAEAKVA
                     LENIYKEIDEIINR"
BASE COUNT          200 a           92 c           78 g          272 t
ORIGIN      
        1 tgtacacttt tgatttttta aacatgtaca tatttaaaaa aataggagag caaagtaaat
       61 aattgatata gttattttga gagtaatcct aggaactatt gtatttatat ttgtctcccc
      121 tacttttaaa tgtcattcat tatacataag cattttgata tagaatttat cacatatgca
      181 aattgaaaac aggttaagac cattttttgt ctcaacctgt tttatttatt atctatttat
      241 aatttcatca atttctttgt aaatattttc taatgctact ttagcttcag ccatttttgc
      301 aaaattatta ctttttagtg cagcttttgc tctatattga ttcatcataa tcgtacgttt
      361 gtagtatggg tttaagctac ctgcagctaa atcatttaca taagattgat caagtagtga
      421 tttcaactct tcagcaattc gtttatcatg aaactcactt tgaattatat agtctttctt
      481 actacttgca tctgctttat ttccttctat agttgctatc gcagttgatg ataaaagtaa
      541 tgctaatgtt cctgttaata tatatttttt aaatttcatt gtaaactctc cttaaaagtt
      601 gatacttaac gattatatag tcaattttag gaaagttaca tt
//
LOCUS       DAHHYJ010000293          603 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.303, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000293 DAHHYJ010000000
VERSION     DAHHYJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.303"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>603
                     /locus_tag="NBK22_002721"
     CDS             <1..>603
                     /locus_tag="NBK22_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD2415844.1"
                     /translation="ATYTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEW
                     YRIVTSMFLHFSFEHILMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFN
                     TTTISVGASGAIFGLIGSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVA
                     HIGGFIGGLLITLIGYYYKVNRNIFWILLIGMLVIFIALQ"
BASE COUNT          176 a           59 c          113 g          255 t
ORIGIN      
        1 cggcaacata cacaataata tttgtaaatg tcttaatatg gttatgtatg attttatatt
       61 taaataattt ttcggatgta aaattattag atgttggcgg gttagtgcat tttaatgtcg
      121 tacatggtga atggtatcga attgttacat cgatgttttt acattttagt tttgaacata
      181 tacttatgaa tatgctttca ttatttattt ttggtaaaat agtcgaagca attattggtt
      241 catggcggat gttaactgta tactttattg cagggttgtt tggaaacttt gtatcactat
      301 catttaatac gactacaatt tcagttgggg ctagtggtgc tatatttggt ctgattggat
      361 caatttttgc gatgatgtat gtttcaaaaa catttaacaa aaaaatgtta ggacagttat
      421 taattgcatt agtgatatta gttggtgttt ctctgtttat gtcaaatata aatattgtgg
      481 cgcatattgg aggattcatt ggtggtttat taataacttt aattggctat tactataaag
      541 tgaatcgtaa tattttttgg attttactaa ttggtatgct tgttatattt attgcacttc
      601 aaa
//
LOCUS       DAHHYJ010000294          597 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.304, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000294 DAHHYJ010000000
VERSION     DAHHYJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.304"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..594
                     /locus_tag="NBK22_002722"
     CDS             <1..594
                     /locus_tag="NBK22_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2415845.1"
                     /translation="KRDKGTWIINSGMNIQLKGGALKSREMILYINRNTRTTKGYFIV
                     GEITKDKKGYTHDKDKKYPVKMEHNQIIPTKPIKDEKLKKEIENFKFFVQYGNFKDFK
                     DYKNGDISYNPNVPSYSAKYQLSNDDYNIQQLRKRYDIPTKKAPELLLKGDGDLKGSS
                     IGSKDLEFTFVQNKRENIYFTDSVEFTPSEDTSYESN"
BASE COUNT          264 a           77 c           99 g          157 t
ORIGIN      
        1 aaaagagata aagggacttg gattattaat tctggaatga acatccaatt aaaaggggga
       61 gccttaaaat ccagagagat gatactttac atcaatcgta acactagaac aacgaaagga
      121 tattttatag taggggagat aactaaagat aaaaagggat atacacatga taaagataaa
      181 aaatatcctg tcaagatgga acataatcaa attatcccaa caaaacctat aaaagatgag
      241 aagttaaaaa aagaaattga aaacttcaag ttttttgtac aatatggcaa ctttaaagat
      301 ttcaaagatt ataaaaatgg ggatatttca tacaatccaa acgtaccaag ttattcggca
      361 aaatatcaat taagtaatga tgattataat atccaacaat taagaaaaag atatgatata
      421 ccgactaaaa aagcaccaga actattgttg aaaggtgatg gcgatttaaa aggttcatcc
      481 ataggctcta aagacttaga atttaccttt gtacaaaata aaagagagaa tatctatttt
      541 acagattcgg ttgaatttac tccaagcgag gatacaagtt atgagtcaaa ctaaata
//
LOCUS       DAHHYJ010000295          572 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.305, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000295 DAHHYJ010000000
VERSION     DAHHYJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.305"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            78..>572
                     /locus_tag="NBK22_002723"
     CDS             78..>572
                     /locus_tag="NBK22_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2415846.1"
                     /translation="MIKLIQISNINKSFNKRCVLKNISFDIEQGKCIALIGKNGAGKS
                     TLIDILIGNVNANSGEIFDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENP
                     LPLEEIIELTKFDSSQLNQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEI
                     REYFW"
BASE COUNT          226 a           68 c           84 g          194 t
ORIGIN      
        1 tcatttcaaa tctatgacaa agtgttctat tttaaatcta ataaatttac taatattaaa
       61 ttaaggaaaa aatagaggtg attaaattga ttcaaatatc taatatcaac aagtcattta
      121 ataaaagatg tgttctaaaa aatatttcgt tcgatattga acaaggtaaa tgtatcgctt
      181 taattggaaa aaatggtgct ggaaagtcaa cgttaattga tatattaatt ggtaatgtta
      241 atgctaattc tggtgagata tttgataaag acaagttatt acaaagtgaa aatcgcagta
      301 taatgttcca aaaaacgatg tttccagatc aattaaaagt tattgagatt atcaacttat
      361 atcaatcatt ttacgaaaat ccattaccat tggaagaaat aatagaactg acgaaatttg
      421 attctagtca actgaaccaa tttgtaaata aactttctgg tggtcaacaa cgattactcg
      481 attttgtatt atctttaatc ggacaaccac aattgatctt attagatgaa ccaacatcga
      541 ctatggatat agaaattaga gaatattttt gg
//
LOCUS       DAHHYJ010000296          570 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.306, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000296 DAHHYJ010000000
VERSION     DAHHYJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.306"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            39..359
                     /gene="ytxJ"
                     /locus_tag="NBK22_002724"
     CDS             39..359
                     /gene="ytxJ"
                     /locus_tag="NBK22_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCD2415847.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
BASE COUNT          200 a           71 c          105 g          194 t
ORIGIN      
        1 ctatttaaat gtgataaata tttttagagg tgaatagagt ggctataaag ctaagttcaa
       61 ttgaccaatt tgaacaggtt attgaggaaa ataaatatgt ttttgtatta aaacatagtg
      121 aaacttgtcc aatatcggca aatgcgtacg atcaatttaa taaattttta tatgaacgcg
      181 atatggacgg ttattatttg attgtccaac aagaacgcga tttgtcagat tatattgcta
      241 aaaaaacgaa cgttaaacat gaatcacctc aagcatttta ttttgtaaat ggtgaaatgg
      301 tttggaatcg agaccacggt gatatcaatg tgtcgtcatt agcacaagca gaagaataat
      361 gaaactatag ggttggaaca ttttgcctta cactactaga cgtgaatagc acaacttaaa
      421 ttcgtgtgaa tcagagtagt ttggctataa tgatgttctg accttttatt ttatgtcacc
      481 tttagaagca gttaagttag tactttttta caaacatatg tataatgtat tcgagtattt
      541 ttattgaaaa tattttggaa aacgacgaat
//
LOCUS       DAHHYJ010000297          567 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.307, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000297 DAHHYJ010000000
VERSION     DAHHYJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.307"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            12..431
                     /locus_tag="NBK22_002725"
     CDS             12..431
                     /locus_tag="NBK22_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD2415848.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            458..>567
                     /locus_tag="NBK22_002726"
     CDS             458..>567
                     /locus_tag="NBK22_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415849.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
BASE COUNT          219 a           84 c           97 g          167 t
ORIGIN      
        1 agtagaacaa catgtataaa caacttgaaa aacttattac actgactaac aatgacttaa
       61 acttagtgaa tagaagattt ggacaacgca cggatatcac atctgaacag ctagaacttc
      121 tccgtatttt atttaattac gatcgcttat cacagtatga tttgacgatg aagattagca
      181 gggaacaatc tatagtttca aggtggatta agaaattagt tttgaaaggg tacatcacaa
      241 gtcaacaatc tagcgaagat ttaagatgta aagaattaat tttgactgat caagcacgta
      301 cattaatttc acaaataaat aatgcacgtt gcgaattgat tgaagcaaga tgtcaatgtt
      361 tatcggaagt cgaattagac aatttaaatc aattacttga taagttaaat caacgacgca
      421 tatcgttgta acacgacaat agcaaacaca taatttagtg tcattgtgca cacaacaaaa
      481 attgaatcat gaattacaag caaaagtagc ggtgattgtt aaaattgatg gtaaacaatc
      541 accgctattt ttgcttgtgt atgtata
//
LOCUS       DAHHYJ010000298          549 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.309, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000298 DAHHYJ010000000
VERSION     DAHHYJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.309"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            99..>549
                     /locus_tag="NBK22_002727"
     CDS             99..>549
                     /locus_tag="NBK22_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCD2415850.1"
                     /translation="MCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPS
                     ACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIV
                     DESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANS"
BASE COUNT          209 a           88 c           93 g          159 t
ORIGIN      
        1 ggaaggtaaa acttcctgct ttttttaata cacaaaaagc gcaattgcct ctataattta
       61 aagtgaccaa acccaaacta aaggagacaa gtgcgcctat gtgtaatgat accttagaat
      121 tactaagaat aaaagatgaa aatataaaat atataaacca agaaattgac gtcattatca
      181 aaggaaaaaa agcaacagtg gttaatgctg tactaacgta taagccttcg gcctgttatt
      241 gttgtggagt taaaaatgaa ggacaaattc ataaacatgg taagcgtgtt tctcgtatta
      301 ctttacttaa aactcaaggg tataacacat acctcaactt agctaaacaa cgttttaaat
      361 gcttagaatg caatggcact tttactgcta aaacgtcaat tgttgatgag tcgtgtttta
      421 tctcaagatg tgttactcaa aaagttatag aagaagctac taaagttaaa acagagattg
      481 atactgcaga agataactgt atctctccat ctactgtaag tcgtattaga actaaagcgg
      541 ctaattcat
//
LOCUS       DAHHYJ010000299          547 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.311, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000299 DAHHYJ010000000
VERSION     DAHHYJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.311"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..>547)
                     /locus_tag="NBK22_002728"
     CDS             complement(<1..>547)
                     /locus_tag="NBK22_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD2415851.1"
                     /translation="IDIISTWISLFFPFINWFITKRYVKISREEFENLNIVKPAKKNV
                     FWPVAGISTLFAVTLRKYTHLLDTQLDKKLVIAICCITFIGILIFYVRLIKKSSLNIY
                     NTKNKRSKIILIPTLKNFCFTLFGYVFLIFWTVVFSYALLSMSYQNIIVYFAWITAIM
                     GFFLVNIALIIDKNIHVILKN"
BASE COUNT          215 a           72 c           67 g          193 t
ORIGIN      
        1 taatttttaa gtatgacatg aatgttttta tctataatta aagctatatt cactagaaaa
       61 aaacccatta ttgctgtaat ccaagcaaaa tatactatta tgttttgata actcattgat
      121 aatagagcat atgagaaaac cactgtccaa aaaattaaaa aaacatatcc gaacaatgtg
      181 aaacaaaaat tttttagtgt tggtattaag atgattttcg accttttatt tttagtatta
      241 taaatattta aagatgattt tttaattagg cgtacataaa atattaaaat ccctataaat
      301 gtgatacaac atatggcaat aactaatttt ttatcaagtt gagtgtcaag taaatgcgta
      361 tactttctta atgtaactgc gaataaagta gagatacctg caactggcca aaaaacattc
      421 tttttagcag gtttaacaat atttaaattt tcaaactctt ctctactaat tttgacgtat
      481 cttttggtga tgaaccaatt aataaaagga aaaaacaaac taatccaagt gcttattata
      541 tcgatca
//
LOCUS       DAHHYJ010000300          544 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.312, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000300 DAHHYJ010000000
VERSION     DAHHYJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.312"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>544
                     /locus_tag="NBK22_002729"
     CDS             <1..>544
                     /locus_tag="NBK22_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1181 family transposase"
                     /protein_id="HCD2415852.1"
                     /translation="ELNKYRVQVMNEYRNKKGPDYTIFKNNWKVLLMDTSKTIFSKYR
                     WNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLRKDLRLCNWPKFINRLNSVSKKS
                     VSKGVWKAVKYYRKHQRMLRNTIYYPAFNNGAIEGINNKIKLIKRISFGYRNFNNFKA
                     RIMMIFSLYKGEKKKTTKPNN"
BASE COUNT          222 a           71 c           91 g          160 t
ORIGIN      
        1 gaacttaata agtatcgtgt acaagttatg aatgaatacc gtaataaaaa aggacctgat
       61 tatacaattt ttaagaataa ctggaaagtc ctattgatgg atactagtaa aaccatattt
      121 agtaaataca gatggaataa atcttttaag gcttataaac gctcatctga cattgtagaa
      181 ttcatgcttt caaaagacga tatactacga cactcctacg aacttgtcca aggattacga
      241 aaagacctaa ggttatgtaa ttggcctaaa tttattaatc gtttaaattc agttagtaaa
      301 aagtctgtga gtaagggtgt atggaaagcg gttaaatatt atagaaaaca tcaaaggatg
      361 ttaagaaata caatttatta cccagcattt aataatggtg ctatagaagg aattaataat
      421 aagataaaat taatcaagcg aatttctttt ggttacagaa atttcaacaa ctttaaagca
      481 cgtataatga tgattttcag cttgtacaaa ggagaaaaaa agaagacaac caagcccaat
      541 aatg
//
LOCUS       DAHHYJ010000301          532 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.313, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000301 DAHHYJ010000000
VERSION     DAHHYJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.313"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>532
                     /locus_tag="NBK22_002730"
     CDS             <1..>532
                     /locus_tag="NBK22_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD2415853.1"
                     /translation="LVLLIILIVGYVIMTKTNGRNAQIKDTFNQTLKLYPTKNLDDFY
                     DKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKDMETRGMVLYINRNTRTTKGYYFISEM
                     TDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPNDKLRKEIENFKFFVQYGDFKDINDYK
                     DGDISYNPNVPSYSAKY"
BASE COUNT          221 a           74 c           94 g          143 t
ORIGIN      
        1 cttggtatta ctcattatat tgattgtagg ttatgtcatt atgacaaaaa caaatggtcg
       61 aaacgcccaa attaaagaca catttaatca aacattaaaa ttatatccaa ccaaaaatct
      121 cgatgatttt tacgataaag aaggctttcg agatcaagaa tttaaaaagg gtgataaagg
      181 tacttggata gttaattctg aaatggtaat cgagccaaaa ggtaaggata tggaaacgag
      241 aggaatggtg ctctatatca atcgcaatac tagaaccaca aaagggtatt attttataag
      301 tgaaatgaca gatgacagta acggcagacc aaaggatgat gaaaaaaggt atccggtaaa
      361 aatggaacat aataaaatca taccaacgaa gccactaccg aatgacaagt taagaaaaga
      421 aatcgaaaac tttaaattct ttgtacaata cggtgacttt aaagatatta atgattataa
      481 agatggtgac atttcatata atcctaatgt tccaagttat tccgcaaaat at
//
LOCUS       DAHHYJ010000302          518 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.314, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000302 DAHHYJ010000000
VERSION     DAHHYJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.314"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            149..231
                     /locus_tag="NBK22_002731"
     tRNA            149..231
                     /locus_tag="NBK22_002731"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:182..184,aa:Leu,seq:gag)
     gene            253..326
                     /locus_tag="NBK22_002732"
     tRNA            253..326
                     /locus_tag="NBK22_002732"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:285..287,aa:Gly,seq:tcc)
     gene            445..>518
                     /locus_tag="NBK22_002733"
     rRNA            445..>518
                     /locus_tag="NBK22_002733"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a           88 c          103 g          152 t
ORIGIN      
        1 gttttattat aaatagcgtt gtgttaacaa ctgataacac aaattaattt attgtaaaaa
       61 aacgattaaa agttattgac aaatattttt aaacataata taataaataa cgttctgaaa
      121 aacgaagatg ttttaaatgc ctatatccgc ggtcgtggcg gaatggcaga cgcgctaggt
      181 tgagggccta gtgggagaaa tcccgtggag gttcaagtcc tctcggccgc atcaaaattc
      241 ttaattaaat aagcgggtgt agtttaatgg caaaacctca gccttccaag ctgatgttgt
      301 gggttcgatt cccatcaccc gctccataga taattttaat gaacattgaa aactgaatga
      361 caatatgtca acgttaattc caaaaacgta actattagtt acaaacatta tttagtattt
      421 atgagctaat caaacatcat aatttttatg gagagtttga tcctggctca ggatgaacgc
      481 tggcggcgtg cctaatacat gcaagtcgag cgaacgga
//
LOCUS       DAHHYJ010000303          512 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.315, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000303 DAHHYJ010000000
VERSION     DAHHYJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.315"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            117..434
                     /locus_tag="NBK22_002734"
     CDS             117..434
                     /locus_tag="NBK22_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD2415854.1"
                     /translation="MKSMVEMQREVDKYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
BASE COUNT          217 a           53 c           97 g          145 t
ORIGIN      
        1 taaaataaac aacttgtcat ctagtttagc tagagtcagt agattgcact aaacatgcat
       61 gagacttcaa attgatatat actatattag aaatgcaata taaggagaac gtagaaatga
      121 aatcaatggt agaaatgcaa cgtgaagttg ataaatatat tggacaattt aaaacaggat
      181 atttttcacc attagctaac ttagctagat tgactgaaga agtgggcgaa cttgcacgtg
      241 aaataaatca tacctatggt gaaaaaaaga agaaagattc agaggaagca aatacgatta
      301 aagcagaatt aggtgataat ttatttgtgt tgttatgttt agcgaattca atgggaatag
      361 atatgacaga gagctttaat gaaacaatgg aaaagtttaa tacaagagat aaaaatcgat
      421 tcgaaagaaa gtgaatataa aaatatatgc tttatttgga tttgattaag attaaaaaca
      481 tagaccaatg aattgcatcg catgaaatta ta
//
LOCUS       DAHHYJ010000304          505 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.316, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000304 DAHHYJ010000000
VERSION     DAHHYJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.316"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>505
                     /locus_tag="NBK22_002735"
     rRNA            <1..>505
                     /locus_tag="NBK22_002735"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          141 a           99 c          150 g          115 t
ORIGIN      
        1 tgattattgg aggtagagca ctgtttggac gaggggcccc tctcgggtta ccgaattcag
       61 acaaactccg aatgccaatt aatttaactt gggagtcaga acatgggtga taaggtccgt
      121 gttcgaaagg gaaacagccc agaccaccag ctaaggtccc aaaatatatg ttaagtggaa
      181 aaggatgtgg cgttgcccag acaactagga tgttggctta gaagcagcca tcatttaaag
      241 agtgcgtaat agctcactag tcgagtgaca ctgcgccgaa aatgtaccgg ggctaaacat
      301 attaccgaag ctgtggattg tcctttggac aatggtagga gagcgttcta agggcgttga
      361 agcatgatcg taaggacatg tggagcgctt agaagtgaga atgccggtgt gagtagcgaa
      421 agacgggtga gaatcccgtc caccgattga ctaaggtttc cagaggaagg ctcgtccgct
      481 ctgggttagt cgggtcctaa gctga
//
LOCUS       DAHHYJ010000305          497 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.317, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000305 DAHHYJ010000000
VERSION     DAHHYJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.317"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..410
                     /locus_tag="NBK22_002736"
     CDS             <1..410
                     /locus_tag="NBK22_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD2415855.1"
                     /translation="DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDNDSDSDSDSDSDAGKHTPAKPMSTVKDQHKTAKALPETG
                     SENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT          180 a           98 c           99 g          120 t
ORIGIN      
        1 cagattcaga cagcgactca gactcagata gcgactcaga ttcagacagc gattcagatt
       61 cagatagcga ctcagactca gatagcgact cagactcaga tagcgactca gactcggata
      121 gcgattcaga ttcagacagc gactcagatt cagacagcga ctcagattca gatagcgatt
      181 cggactcaga caacgactca gattcagata gcgattcaga ttcagatgca ggtaaacata
      241 ctccggctaa accaatgagt acggttaaag atcagcataa aacagctaaa gcattaccag
      301 aaacaggtag tgaaaataat aattcaaata atggcacatt attcggtgga ttattcgcgg
      361 cattaggatc attattgtta ttcggtcgtc gtaaaaaaca aaataaataa tatgattaac
      421 ttaaccaggt ccatgtggcc tggttttttc tgtttagaaa ttcccagata taaaacaaaa
      481 aatctttaag gaataga
//
LOCUS       DAHHYJ010000306          489 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.318, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000306 DAHHYJ010000000
VERSION     DAHHYJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.318"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>489
                     /locus_tag="NBK22_002737"
     CDS             <1..>489
                     /locus_tag="NBK22_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCD2415856.1"
                     /translation="DKDIYVFNKSKATTITPFNAKYNIHYLSDDELLKKDIKGSYFVK
                     DKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIKQMNIEVPLSALLIVYFIYYIFE
                     KNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTLIISQILLTTSVLWILNYTGNLD
                     LF"
BASE COUNT          193 a           54 c           64 g          178 t
ORIGIN      
        1 tagataaaga tatatatgta tttaataaat caaaagcaac aactatcact ccttttaacg
       61 ctaaatataa cattcattat ttaagtgacg acgaattatt aaaaaaagat atcaaaggaa
      121 gttattttgt aaaagacaaa aattttgacg tgtctaaatt cataaatttt ttaaaagaat
      181 atggtgttac tgctgaatca tacaaaatag atcatatgat gattgccgtt ggtgtcatta
      241 aacaaatgaa tatagaagtt ccgttatccg cacttttaat cgtttatttt atttattata
      301 ttttcgaaaa gaacattaat ttcaaagcgt atgcgattaa gtatttaaat ggttttacat
      361 taagaaaaat aatttttgaa aatttttcaa aaaaatgcac gtattgggta acgttaatca
      421 taagtcaaat tcttttaact acaagtgtac tttggatatt aaattacacg ggtaatttag
      481 atttattca
//
LOCUS       DAHHYJ010000307          485 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.319, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000307 DAHHYJ010000000
VERSION     DAHHYJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.319"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..238
                     /gene="sec3"
                     /locus_tag="NBK22_002738"
     CDS             <1..238
                     /gene="sec3"
                     /locus_tag="NBK22_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type C3"
                     /protein_id="HCD2415857.1"
                     /translation="KARNFLINKKNLYEFNSSPYETGYIKFIENNGNTFWYDMMPAPG
                     DKFDQSKYLMMYNDNKTVDSKSVKIEVHLTTKNG"
     gene            complement(405..>485)
                     /locus_tag="NBK22_002739"
     CDS             complement(405..>485)
                     /locus_tag="NBK22_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCD2415858.1"
                     /translation="SFLKIYKDNKTVDSTQFHLDVEISKR"
BASE COUNT          185 a           55 c           78 g          167 t
ORIGIN      
        1 aaaagctagg aattttttaa ttaataaaaa aaatttgtat gagtttaaca gttcaccata
       61 tgaaacagga tatataaaat ttattgaaaa taacggcaat actttttggt atgatatgat
      121 gcctgcacca ggcgataagt ttgaccaatc taaatattta atgatgtaca acgacaataa
      181 aacggttgat tctaaaagtg tgaagataga agtccacctt acaacaaaga atggataatg
      241 ttaatccgat tttgatataa aaagtgaaag tattagatat atttgaaagg taagtacttc
      301 ggtgcttgcc tttttaggat gcatatatat agattaaacc gcacttctat attaatagaa
      361 agtgcggtta tttatacagt taatctaaac tataataatt ggaatcatct ttttgaaatt
      421 tcgacatcta gatgaaattg tgttgaatca acagttttat tatctttgta aatttttaag
      481 aagct
//
LOCUS       DAHHYJ010000308          485 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.320, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000308 DAHHYJ010000000
VERSION     DAHHYJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.320"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..379
                     /locus_tag="NBK22_002740"
     CDS             <1..379
                     /locus_tag="NBK22_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD2415859.1"
                     /translation="ILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQSQIT
                     LSDEYIRKLKLDKDDLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKVSIV
                     DSYFNNKKQRGSNYDTKLLENRI"
     gene            348..>485
                     /locus_tag="NBK22_002741"
     CDS             348..>485
                     /locus_tag="NBK22_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415860.1"
                     /translation="MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPEDV
                     KP"
BASE COUNT          197 a           65 c           60 g          163 t
ORIGIN      
        1 gatactctat acatcgcact atattgaaga agtcgaacgt atgtcagaca aaattattct
       61 cattgaaaat ggagaaataa tacttaatga ttcaacgtca catattagaa ccaatcagca
      121 atctcagatt acgttatccg atgaatatat aagaaagtta aaactagata aagatgattt
      181 agttattcaa aaaaatcata atggcactat caaaattatt acttcaaatg taaatgatac
      241 gattttatat cttcaacaac ttcatattaa tttggatgat attgaaatac aaaaagtctc
      301 aattgttgat tcatacttca acaataaaaa gcaaagggga tctaattatg atactaagtt
      361 acttgaaaat cgaatttaaa gttataatgc gtaaaaaaac aacattaata ttatctattt
      421 tatttcctgt tatattctat atattattta cttcgatatt ggaattgccg gaagatgtta
      481 aacct
//
LOCUS       DAHHYJ010000309          485 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.321, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000309 DAHHYJ010000000
VERSION     DAHHYJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.321"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..482
                     /locus_tag="NBK22_002742"
     CDS             <1..482
                     /locus_tag="NBK22_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD2415861.1"
                     /translation="SKLYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFFNYTKPGS
                     DDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEGLEPWTSCEFDFTSEGK
                     LKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIDQYIKEQDEAEI"
BASE COUNT          188 a           40 c          106 g          151 t
ORIGIN      
        1 taagcaaatt atataatgag attgcgaatg agattagcag tatgataccg gtagagtggg
       61 aaaaagtata tacaatggct tatatagatg atggaggagg tgaagtattc tttaattata
      121 ctaaaccagg tagtgatgac ttgaattatt acaccgatat acctaaggag tataacatct
      181 ctgtgcaagt atttgatgat ttatggatgg atttatatga tttgtttgag gaattaagag
      241 atttatttaa agaagaagga cttgaaccat ggacatcatg tgaatttgac tttacaagcg
      301 aaggtaaatt aaaagtttca tttgattata tagattggat aaatacagag tttgatcaat
      361 taggccgtga aaattattat atgtataaaa aatttggggt tttaccagaa atggaatatg
      421 aaatggaaga aattaaagaa atcgatcaat atattaaaga gcaagatgaa gctgaaatat
      481 agggg
//
LOCUS       DAHHYJ010000310          479 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.322, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000310 DAHHYJ010000000
VERSION     DAHHYJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.322"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>479
                     /locus_tag="NBK22_002743"
     rRNA            <1..>479
                     /locus_tag="NBK22_002743"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          139 a           89 c          142 g          109 t
ORIGIN      
        1 gtaataatcc tgtagtcgaa aatgttgtct ctcttgagtg gatcctgagt acgacggagc
       61 acgtgaaatt ccgtcggaat ctgggaggac catctcctaa ggctaaatac tctctagtga
      121 ccgatagtga accagtaccg tgagggaaag gtgaaaagca ccccggaagg ggagtgaaat
      181 agaacctgaa accgtgtgct tacaagtagt cagagcccgt taatgggtga tggcgtgcct
      241 tttgtagaat gaaccggcga gttacgattt gatgcaaggt taagcagtaa atgtggagcc
      301 gtagcgaaag cgagtctgaa tagggcgttt agtatttggt cgtagacccg aaaccaggtg
      361 atctaccctt ggtcaggttg aagttcaggt aacactgaat ggaggaccga accgacttac
      421 gttgaaaagt gagcggatga actgagggta gcggagaaat tccaatcgaa cctggagat
//
LOCUS       DAHHYJ010000311          478 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.323, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000311 DAHHYJ010000000
VERSION     DAHHYJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.323"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
BASE COUNT          197 a           56 c           61 g          164 t
ORIGIN      
        1 taacaactca atattaaatg ccttatgtgt taattgtcta atttgctata aaacttagta
       61 agtcatatag tcgattccta tcgtatagag atataataat aggcaagtac cgaagtacct
      121 gcctaaataa caacaagatt aacatgtgaa taataatgga aataaaaagt cagcccggag
      181 gctaacttat gaatagatga aaatttgaac acattcctgt gtctaaaatg attatagcat
      241 aaatgacgaa tatttctatc ttaaaattaa tatattgtaa taataaaatt ttatatcttc
      301 gttaataatt agttaattga tttacataaa taataattgt aaatttaatt tgtaatcgat
      361 tgcaaaataa attataggag aacttaaaat gaataaaaaa ctattaacaa aaacattgat
      421 agcaagtgct ttagttttaa caacagtagg ttcaggtttt cattcttctt caaattat
//
LOCUS       DAHHYJ010000312          462 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.324, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000312 DAHHYJ010000000
VERSION     DAHHYJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.324"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..157)
                     /locus_tag="NBK22_002744"
     CDS             complement(<1..157)
                     /locus_tag="NBK22_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCD2415862.1"
                     /translation="MNKSRFISCVILIFALILVLFTPNVLAESQPDPTPDELHKASKF
                     TGLMENMK"
BASE COUNT          164 a           70 c           53 g          175 t
ORIGIN      
        1 ctttcatatt ttccatcaaa ccagtgaatt tactcgcttt gtgcaactca tctggcgtag
       61 ggtctggttg gctctctgct aatacgttgg gtgtaaaaag aactagtata agtgcgaata
      121 tcaaaattac gcatgaaata aatcgactct tattcatttt tatctccttc atccaacatt
      181 cccaaaagta tctagataca ctttaaatat atgttataat cttaaatatt caattaaaaa
      241 aatattaaaa gaaaattaat tatatttaat gatttcgcgt tatttctaag aattaagtta
      301 tgctttgaaa attaattaac tatacttcat ctgaacttat atacaaaact ctttttcaag
      361 gagaaacaaa ggatttctaa gcatcttgaa ttaataacat tgtttatata ttcacacttt
      421 tataattaat gcattcattt ttgcaaattc taaaattggt tt
//
LOCUS       DAHHYJ010000313          460 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.325, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000313 DAHHYJ010000000
VERSION     DAHHYJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.325"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>460
                     /locus_tag="NBK22_002745"
     CDS             <1..>460
                     /locus_tag="NBK22_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YydG family peptide radical SAM peptide
                     maturase"
                     /protein_id="HCD2415863.1"
                     /translation="SILGKKITKFPMISVGAAKDKINEKDIHNFYNIKKDKNMLYCPG
                     YEIVYHHDGEIYPCCSPAIFETQLSLRESKNQSFERTVEKLNSNLLLYIIRKEGFNWF
                     LDILKEKSLLDTFEIPENFPSVCSICGSLFNTTEKINFFKPYMERYYNETV"
BASE COUNT          186 a           56 c           65 g          153 t
ORIGIN      
        1 tcaattttag gtaaaaagat aacaaaattc ccaatgattt ctgtaggagc cgcaaaagat
       61 aaaataaacg aaaaggatat tcataatttt tacaatatta aaaaagacaa aaatatgctt
      121 tactgtcctg gatatgagat agtttaccat catgatggag aaatatatcc atgctgttca
      181 ccagcaatat ttgaaacaca actttctctt agggaatcta aaaatcaatc ttttgaaaga
      241 actgttgaaa aattaaattc aaatttgtta ttatatataa ttagaaaaga aggttttaat
      301 tggtttttag atattttaaa agaaaagagt ttactagata cttttgaaat acctgagaac
      361 ttcccatcag tttgtagtat atgtgggagt ttattcaata ctactgaaaa aattaatttt
      421 ttcaaacctt acatggaaag atactataat gaaaccgtta
//
LOCUS       DAHHYJ010000314          455 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.326, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000314 DAHHYJ010000000
VERSION     DAHHYJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.326"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            41..238
                     /locus_tag="NBK22_002746"
     CDS             41..238
                     /locus_tag="NBK22_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415864.1"
                     /translation="MNCYDEIFNTIKKLIENKEISSYQINKDTGISYGNINAMRRGER
                     RIENLSLKNSKILYEYAKKVL"
BASE COUNT          182 a           44 c           72 g          157 t
ORIGIN      
        1 tgatattcta aaaaattaaa aatttcgcct catctaattt atgaattgct atgatgaaat
       61 attcaataca atcaaaaagt tgatagaaaa caaagagata tcgagttatc aaattaataa
      121 agatactggg ataagttacg gtaatattaa tgctatgcgc cgtggagaaa gaagaataga
      181 aaatttaagc ttaaagaatt caaaaatctt atatgaatat gcgaaaaagg tattataata
      241 taagcattga gttcaatagg cgtataataa ctgcccaatc tagtgactga gtggatgtta
      301 ctatagtatt aaaagttttc taaaagtgaa agaaatcatg tttagtgatt tacttatgtt
      361 tttcgaaata gtataataat tttatataat atatagaact tcagtatacc ttttgagtat
      421 atatggagtt ctttcttttt tacaagatgt atatg
//
LOCUS       DAHHYJ010000315          416 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.327, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000315 DAHHYJ010000000
VERSION     DAHHYJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.327"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..256
                     /locus_tag="NBK22_002747"
     CDS             <1..256
                     /locus_tag="NBK22_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415865.1"
                     /translation="PRTNNRSEKGIIFWDNINSMGEKEFIEKVKFEKEEELLEKTIQQ
                     NYFLAKTASMKYSIIRNVFIISALGYLCLLFSSIFQIICS"
     gene            295..>416
                     /locus_tag="NBK22_002748"
     CDS             295..>416
                     /locus_tag="NBK22_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCD2415866.1"
                     /translation="MATIGFEEKLWQAADKLRGSMDAAEYKNVALGIIFLKYVS"
BASE COUNT          175 a           36 c           72 g          133 t
ORIGIN      
        1 tccaagaaca aataatagaa gcgaaaaagg aattattttt tgggataata taaatagtat
       61 gggagaaaaa gaatttatag agaaagtgaa atttgaaaaa gaggaagaat tacttgaaaa
      121 aacgatacaa caaaattatt ttttagcaaa aactgcatct atgaaatatt caatcattag
      181 aaatgttttt ataatttcag cattaggcta tctctgttta ttattttcaa gtatctttca
      241 gattatatgc tcttgaattt tcagttttaa catgaaaagg agaaaaataa aaatatggca
      301 acaattggat ttgaagaaaa attatggcaa gctgcggata aattacgagg gagtatggat
      361 gcagcagaat ataaaaatgt agcattaggt attatatttt taaaatatgt ttctga
//
LOCUS       DAHHYJ010000316          389 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.329, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000316 DAHHYJ010000000
VERSION     DAHHYJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.329"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..168
                     /locus_tag="NBK22_002749"
     CDS             <1..168
                     /locus_tag="NBK22_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415867.1"
                     /translation="LLTNNTPYSISKNSGVPRQTVTDLKVGKTKIKEAKFKTIIKLYE
                     YQRTLENKTEC"
BASE COUNT          165 a           48 c           54 g          122 t
ORIGIN      
        1 ttattaacta acaatacacc atatagtatt tcaaaaaact caggtgtacc acgtcaaaca
       61 gttactgatt taaaggtagg taaaactaaa ataaaagaag ctaaatttaa aacgataatc
      121 aagttatatg aatatcaaag aacattagaa aataaaacag aatgttaaca aaaatatttt
      181 gttaggttag gtggacaaga aattaaattt ctaatgattt catggttgtc tgtccacgtt
      241 ttgtccacct caataagata atctcttagt tttagaaatt gtacatggtt aaataatcaa
      301 aaatgcatat tatgaaatta tcattgccat taaatcttga atgatcttta agcgatgata
      361 taaaaggctg aacacagtaa ataagttaa
//
LOCUS       DAHHYJ010000317          376 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.330, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000317 DAHHYJ010000000
VERSION     DAHHYJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.330"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..>376)
                     /locus_tag="NBK22_002750"
     rRNA            complement(<1..>376)
                     /locus_tag="NBK22_002750"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           80 a          120 c           77 g           99 t
ORIGIN      
        1 gcgttagctg cagcactaag gggcggaaac cccctaacac ttagcactca tcgtttacgg
       61 cgtggactac cagggtatct aatcctgttt gatccccacg ctttcgcaca tcagcgtcag
      121 ttacagacca gaaagtcgcc ttcgccactg gtgttcctcc atatctctgc gcatttcacc
      181 gctacacatg gaattccact ttcctcttct gcactcaagt tttccagttt ccaatgaccc
      241 tccacggttg agccgtgggc tttcacatca gacttaaaaa accgcctacg cgcgctttac
      301 gcccaataat tccggataac gcttgccacc tacgtattac cgcggctgct ggcacgtagt
      361 tagccgtggc tttctg
//
LOCUS       DAHHYJ010000318          327 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.331, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000318 DAHHYJ010000000
VERSION     DAHHYJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.331"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>327
                     /locus_tag="NBK22_002751"
     CDS             <1..>327
                     /locus_tag="NBK22_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Csa1 family protein"
                     /protein_id="HCD2415868.1"
                     /translation="SLYPTKNLEDFYDKEGFRDQEFEKGDKGNWIVDSEMVIELKDKK
                     MESRSMVLYINRNTRTTKGNFIVRELWEDSKGYAQSKDTKYPVKMEHNRIIPTKPIAD
                     DKLRKE"
BASE COUNT          135 a           36 c           73 g           83 t
ORIGIN      
        1 gagcctgtat ccaactaaaa atcttgaaga cttttatgat aaggaaggct ttcgagatca
       61 agagtttgaa aagggcgaca aaggcaattg gattgttgat tctgagatgg taattgagct
      121 gaaggataaa aaaatggaat ctagaagtat ggtgctctat ataaatcgta atactagaac
      181 aacgaaaggt aattttattg ttagagaatt gtgggaagat agtaaagggt atgcacaaag
      241 taaagataca aaatatccag tgaaaatgga acataatcga attatcccaa cgaaaccgat
      301 agctgatgat aaattaagaa aagaaat
//
LOCUS       DAHHYJ010000319          319 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.332, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000319 DAHHYJ010000000
VERSION     DAHHYJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.332"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..307
                     /locus_tag="NBK22_002752"
     CDS             <1..307
                     /locus_tag="NBK22_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD2415869.1"
                     /translation="VSKDIFKNSWFKVYRMFDELRETFKEEGLEPWTSCEFDFTRDGK
                     LNVSFDYIDWINTEFDQLGRQNYYMYKKFGVIPEMEYEMEEVKEIEQYIKEQEEAEQ"
BASE COUNT          129 a           29 c           66 g           95 t
ORIGIN      
        1 tgtctcaaaa gatattttta agaattcatg gtttaaagtt tatcgaatgt ttgatgagtt
       61 aagagaaact tttaaagaag aagggcttga accatggaca tcatgcgaat ttgactttac
      121 aagagatggc aaattgaatg tatcttttga ttatatagat tggataaata cagagtttga
      181 tcaattgggc cgtcaaaatt attatatgta caaaaaattt ggggttatac cagaaatgga
      241 atatgaaatg gaagaagtta aagaaatcga acaatatatt aaagagcaag aagaagctga
      301 acaatagagg tgataacat
//
LOCUS       DAHHYJ010000320          315 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.333, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000320 DAHHYJ010000000
VERSION     DAHHYJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.333"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>315
                     /locus_tag="NBK22_002753"
     rRNA            <1..>315
                     /locus_tag="NBK22_002753"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           99 a           61 c           87 g           68 t
ORIGIN      
        1 taagggcgca cggtggatgc cttggcacta gaagccgatg aaggacgtta ctaacgacga
       61 tatgctttgg ggagctgtaa gtaagctttg atccagagat ttccgaatgg ggaaacccag
      121 catgagttat gtcatgttat cgatatgtga atacatagca tatcagaagg cacacccgga
      181 gaactgaaac atcttagtac ccggaggaag agaaagaaaa ttcgattccc ttagtagcgg
      241 cgagcgaaac gggaagagcc caaaccaaca agcttgcttg ttggggttgt aggacactct
      301 atacggagtt acaaa
//
LOCUS       DAHHYJ010000321          307 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.334, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000321 DAHHYJ010000000
VERSION     DAHHYJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.334"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..>307)
                     /locus_tag="NBK22_002754"
     CDS             complement(<1..>307)
                     /locus_tag="NBK22_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415870.1"
                     /translation="KTDENGKYRFDNLDSGKYKVIFEKPAGLTQTVTNTTEDDKDADG
                     GEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS"
BASE COUNT           72 a           64 c           55 g          116 t
ORIGIN      
        1 tctgagtcgc tatctgaatc tgaatcactg tctgagtctg agtcgctgtc tgagtctgag
       61 tcgctgtctg agtcactatc tgagtctgaa tcgctgtctg atgtatcttc ttcgaagtat
      121 ccgttatcaa gtgtgaaatc atcatgatcc gtaattgtta cgtcaacttc gccaccatct
      181 gcatctttat catcttcagt tgtatttgta actgtttgtg ttaagccagc aggcttttca
      241 aaaataactt tgtatttacc gctatctaaa ttatcgaaac gatatttacc attttcatct
      301 gttttag
//
LOCUS       DAHHYJ010000322          306 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.335, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000322 DAHHYJ010000000
VERSION     DAHHYJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.335"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..>306)
                     /gene="deoC"
                     /locus_tag="NBK22_002755"
     CDS             complement(<1..>306)
                     /gene="deoC"
                     /locus_tag="NBK22_002755"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCD2415871.1"
                     /translation="PLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRFDDVQQ
                     DIEAVVKAAKGHTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGGATAED
                     "
BASE COUNT           70 a           81 c           50 g          105 t
ORIGIN      
        1 gtcttctgca gtcgcaccgc cacctgcaaa acctgttgaa gttttaacga agtccgcacc
       61 agccgctttt gttaattcac tcgcttttac aatttcgtca tggtccaaca ataccgtctc
      121 aataatcact tttactgtgt gacctttcgc agctttaacc actgcttcaa tgtcttgttg
      181 tacatcatca aaacgtccat cttttaatgc gccgatgttg atgaccatgt caatttcatc
      241 tgcaccattt tgaattgcat cttctgtttc aaatgctttc gttgcagttg tcgacgcacc
      301 taatgg
//
LOCUS       DAHHYJ010000323          294 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.336, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000323 DAHHYJ010000000
VERSION     DAHHYJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.336"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            52..228
                     /locus_tag="NBK22_002756"
     CDS             52..228
                     /locus_tag="NBK22_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCD2415872.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
BASE COUNT          121 a           45 c           52 g           76 t
ORIGIN      
        1 tgtgcaattg actataaagt tccgttgata tttggaggga gggaaataca gatgtctaaa
       61 acagtagtac gtaaaaatga atcacttgaa gatgcgttac gtagatttaa acgttcagtt
      121 tctaaaagtg gaacaatcca agaagtacgt aaacgtgaat tttacgaaaa accaagcgta
      181 aaacgtaaaa agaaatcaga agctgcacgt aaacgtaaat tcaaataatt aatacctctg
      241 ttgactccct caacacgaat attaattata taaaacaaac atcacaagtt agtg
//
LOCUS       DAHHYJ010000324          281 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.337, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000324 DAHHYJ010000000
VERSION     DAHHYJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.337"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>281
                     /gene="sel"
                     /locus_tag="NBK22_002757"
     CDS             <1..>281
                     /gene="sel"
                     /locus_tag="NBK22_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type L"
                     /protein_id="HCD2415873.1"
                     /translation="VFGISYKYGSNSRTIYGGVTKAENNKLDSPRIIPINLIINGKHQ
                     TVTTKSVSTDKKMVTAQEIDVKLRKYLQDEFNIYGHNDTGKGKEYGTSS"
BASE COUNT          117 a           39 c           44 g           81 t
ORIGIN      
        1 gtttttggta taagctataa atatggttct aactctcgta ctatatatgg tggtgttact
       61 aaagcagaaa acaataaatt agattcgcca agaataatac ctataaattt aattatcaat
      121 ggcaagcatc aaacagttac aactaaaagt gtttctacag ataaaaaaat ggttaccgca
      181 caagaaatag atgtcaaact aagaaaatac ttgcaagatg aatttaatat ttatggacac
      241 aatgatactg gtaaaggtaa agaatacggc acttcttcaa a
//
LOCUS       DAHHYJ010000325          274 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.340, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000325 DAHHYJ010000000
VERSION     DAHHYJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.340"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>274
                     /locus_tag="NBK22_002758"
     CDS             <1..>274
                     /locus_tag="NBK22_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive phage resistance protein"
                     /protein_id="HCD2415874.1"
                     /translation="INRLIKIINVLGYKYNKETGKITKNESEVNLSTIKSLAIKFDIE
                     YVLKECNRIEKEALTDPEDAITSAKSMVESTLKYILDSEGEKFNNN"
BASE COUNT          129 a           18 c           45 g           82 t
ORIGIN      
        1 aaattaatag attaataaaa ataattaatg tattaggtta taaatataat aaagagacag
       61 gaaaaataac taaaaatgaa agtgaagtta atttaagtac tattaaatct ttagcaataa
      121 aatttgatat tgaatatgta ttaaaagaat gtaatagaat tgaaaaagag gcactaacgg
      181 atccagagga cgcaattaca tctgcaaaat cgatggttga gagtactttg aagtatattt
      241 tagattctga aggagaaaaa tttaataata atga
//
LOCUS       DAHHYJ010000326          250 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.342, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000326 DAHHYJ010000000
VERSION     DAHHYJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.342"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>250
                     /locus_tag="NBK22_002759"
     CDS             <1..>250
                     /locus_tag="NBK22_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCD2415875.1"
                     /translation="EKIIPEIKEDGDSDLTIEEIDLIGSHLDKEIEDLNHSIENEDCA
                     QIRKQTRKKRTEIKKFKKKFDDYSERKNKYEEQKSILK"
BASE COUNT          126 a           22 c           43 g           59 t
ORIGIN      
        1 aagaaaaaat aataccagag ataaaagaag atggagatag cgatttaaca atagaagaaa
       61 tagatttaat tggtagtcat ttagataaag aaatcgaaga tttaaatcat tctattgaga
      121 acgaagattg tgctcaaatt agaaaacaga cccgtaaaaa aagaactgag attaagaagt
      181 tcaaaaagaa atttgatgat tattccgaaa gaaaaaataa atatgaagaa caaaaatcga
      241 ttcttaaaga
//
LOCUS       DAHHYJ010000327          216 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.345, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000327 DAHHYJ010000000
VERSION     DAHHYJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.345"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            67..>216
                     /locus_tag="NBK22_002760"
     CDS             67..>216
                     /locus_tag="NBK22_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5079 family protein"
                     /protein_id="HCD2415876.1"
                     /translation="MTTKENIDTLRKPGAQALSLISLFLILFSCLTFFFGLDYERFPN
                     YLKITT"
BASE COUNT           77 a           25 c           32 g           82 t
ORIGIN      
        1 agaatctaag tatatagctt taaatacgta atatgctatt taaaaaataa tgtataggag
       61 atagatatga ctactaaaga aaatattgat actcttcgaa agccaggtgc acaagcctta
      121 agtttaatat cattgttttt gatacttttt tcatgtctga ctttcttttt tggtttagat
      181 tatgaaaggt ttcctaatta tttaaagata acgaca
//
LOCUS       DAHHYJ010000328          209 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.348, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000328 DAHHYJ010000000
VERSION     DAHHYJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.348"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
BASE COUNT           90 a           32 c           21 g           66 t
ORIGIN      
        1 tataaagcac aataaaatag cacttttaca aatatattat tagaaaagtt gaaacaaata
       61 tataacccca aataatataa tcattatcat taatgtaata ccaaaaaaag aagttaaaca
      121 atgcatagtt gcttaacttc caatattgaa ctcatcatta caatttgaca tagagtctat
      181 taaagcgtgt gccatttgag tccactttt
//
LOCUS       DAHHYJ010000329          202 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.denovo.349, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000329 DAHHYJ010000000
VERSION     DAHHYJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.denovo.349"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..>202
                     /locus_tag="NBK22_002761"
     rRNA            <1..>202
                     /locus_tag="NBK22_002761"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           45 c           59 g           50 t
ORIGIN      
        1 gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa
       61 cgagcgcaac ccttaagctt agttgccatc attaagttgg gcactctaag ttgactgccg
      121 gtgacaaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgatttgggc
      181 tacacacgtg ctacaatgga ca
//
LOCUS       DAHHYJ010000330         4816 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.guided.165, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000330 DAHHYJ010000000
VERSION     DAHHYJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.guided.165"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            213..1169
                     /locus_tag="NBK22_002762"
     CDS             213..1169
                     /locus_tag="NBK22_002762"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD2415877.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPFNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            1369..1848
                     /locus_tag="NBK22_002763"
     CDS             1369..1848
                     /locus_tag="NBK22_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD2415878.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1863..2702
                     /gene="fakB2"
                     /locus_tag="NBK22_002764"
     CDS             1863..2702
                     /gene="fakB2"
                     /locus_tag="NBK22_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCD2415879.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGASYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSIGVAHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2788..3321
                     /gene="msrA"
                     /locus_tag="NBK22_002765"
     CDS             2788..3321
                     /gene="msrA"
                     /locus_tag="NBK22_002765"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD2415880.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            3314..3742
                     /gene="msrB"
                     /locus_tag="NBK22_002766"
     CDS             3314..3742
                     /gene="msrB"
                     /locus_tag="NBK22_002766"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD2415881.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMLRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3754..4254
                     /locus_tag="NBK22_002767"
     CDS             3754..4254
                     /locus_tag="NBK22_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD2415882.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            4254..4475
                     /locus_tag="NBK22_002768"
     CDS             4254..4475
                     /locus_tag="NBK22_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCD2415883.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4667..>4816
                     /locus_tag="NBK22_002769"
     CDS             4667..>4816
                     /locus_tag="NBK22_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD2415884.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQETNSSKRVHLKRWQFISILI
                     GTILIT"
BASE COUNT         1775 a          705 c          860 g         1476 t
ORIGIN      
        1 gttatagatt tgggtaaaca attaccaatt ggaaacatat atcacgttac gatggggtag
       61 gtacttaatc agcattttat aaataaagta acggaattca tgatattaat atcatattcc
      121 taaaatgagt gataacaaaa tgctacataa agttaagtta tatcaaacta aatatacata
      181 ctataaataa tgaaaatgag gtgttatcgc atatgttgaa ttcatttgat gcagcatatc
      241 acagtctttg tgaagaagtt ttagaaatag gaaatacacg aaatgatcgc acaaatacag
      301 gtacgatttc gaaatttggt catcaacttc gctttgactt atctaaagga tttccactat
      361 taacgacaaa gaaagtttct tttaaattag tagcaaccga attattatgg ttcattaaag
      421 gagatacaaa catccaatac ttattaaaat ataataataa tatatggaac gaatgggctt
      481 ttgaaaatta tatcaaatca gacgagtata aaggtccaga tatgacagat ttcgggcatc
      541 gtgcattgag tgatcctgaa tttaacgaac aatataaaga acaaatgaaa caatttaagc
      601 aacgtattct tgaagatgat acatttgcga agcaattcgg ggatttagga aatgtttatg
      661 gtaaacaatg gcgagattgg gttgataaag atggtaatca ttttgatcaa cttaaaacag
      721 taattgaaca aattaagcat aatccagatt caaggcgaca catcgtatct gcatggaatc
      781 caacagaaat tgatacaatg gcacttccgc cttgtcatac catgttccag ttttatgtcc
      841 aagatggtaa gttaagttgc cagttatacc aacgtagcgc agatatcttt ttaggtgtgc
      901 catttaatat cgcaagctac gctttattga cacaccttat tgccaaagaa tgtggacttg
      961 aagtgggtga atttgtgcat acatttggag atgcacatat ttattcaaat catattgatg
     1021 cgattcaaac acaattagca cgtgaaagct tcaatcctcc aacattaaaa attaacagtg
     1081 acaagtctat tttcgacata aattatgaag atttggaaat tgttgactat gaatcacatc
     1141 cagcaataaa agctccaata gcagtgtagt cattgcatag ttagctaacc atatagacat
     1201 caaaatgaca tcatagtatt ttcaagtgca aaaaagtact tttttgtgtt aaacgttttc
     1261 ataaattatg caaaatcatt atttctatca cactttatga taaaaattgt gttaaattaa
     1321 agataactta gtaataaaaa atgaaatgat agaagaagga ggataattat gactttatcc
     1381 attctagttg cacatgactt gcaacgagta attggttttg aaaatcaatt accttggcac
     1441 ctaccaaatg atttgaagca tgttaaaaaa ttatcaacag gtcatacttt agtaatgggt
     1501 cgtaagacat ttgaatcgat tggtaaacca ctaccgaatc gtcgaaatgt tgtacttact
     1561 tcagatacaa gtttcaacgt agagggcgtt gatgtaattc actctattga agatatttac
     1621 caactaccgg gccatgtttt catatttgga gggcaaacat tatttgaaga aatgattgat
     1681 aaagtggacg acatgtatat tactgttatt gaaggtaaat tccgtggtga tacgttcttt
     1741 ccaccttata catttgaaga ctgggaagtt gcctcttcag ttgaaggtaa actagatgag
     1801 aaaaatacaa ttccacatac ctttctacat ttaattcgta aaaaataagg gggaaaacga
     1861 ccatgacaaa acagattata gtaacagact caacatccga tttatctaaa gaatacttag
     1921 aagcaaacaa cattcatgta attcctttaa gtttaactat tgaaggagct tcatacgttg
     1981 accaagtaga tattacatca gaagaattta ttaatcatat tgaaaatgat gaagatgtaa
     2041 agacaagtca gccagccata ggtgaattta tatctgctta tgaagaacta ggaaaagatg
     2101 gctctgaaat cataagtatt catctttctt caggattaag tggtacatat aacactgctt
     2161 accaagcaag tcaaatggta gatgctaatg taactgttat tgattcaaaa tctatttctt
     2221 ttggtttagg gtatcaaata caacacctag tagagcttgt aaaagaaggt gtctcaactt
     2281 ctgaaatagt taaaaagtta aatcatttaa gagaaaacat taaattattt gtagttatag
     2341 ggcaattgaa tcaattaatt aaaggtggca gaattagtaa aacaaaaggt ttgattggta
     2401 atcttatgaa aattaaacca attggtacac tagatgatgg tcgcttagag cttgtgcaca
     2461 atgcgagaac tcaaaattct agtatccaat acttgaaaaa ggaaattgct gaatttatag
     2521 gagatcatga aatcaaatcc attggtgtcg cacatgctaa cgtcattgaa tatgttgata
     2581 aattgaagaa agtttttaat gaagcttttc atgtgaataa ttacgatata aatgtaacta
     2641 caccagttat ttctgcacat actggtcaag gtgcgattgg cctcgtagtc cttaagaagt
     2701 aaatttaatc ttttcagtgt taattacttc catttcaatc ctttatagac taaatttata
     2761 attagataga tagaggaggt aattcatatg acaaaagaat atgcaacatt agcaggagga
     2821 tgtttctggt gcatggttaa accatttaca tcatatccag gcatcaagtc agtcgtatct
     2881 ggttatagtg gcggtcatgt tgacaaccca acttatgaac aggtatgtac gaatcaaacc
     2941 ggccatgtcg aagcagtaca aattacgttt gatccagagg ttacttcctt tgaaaatata
     3001 ttagacatat atttcaaaac atttgaccca actgatgatc aagggcaatt tttcgataga
     3061 ggcgaaagct atcaaccagt cattttctat catgatgaac atcagaaaaa ggctgctgag
     3121 tttaaaaagc aacaattaaa tgaacaaggt attttcaaga aaccagtgat tacacctatt
     3181 aaaccatata aaaatttcta tccagctgaa gactaccatc aagattatta caaaaagaac
     3241 ccggtacatt attaccaata tcaacgtggt tcaggtagaa aagcgtttat agaatcacat
     3301 tgggggaatc aaaatgctta aaaaagataa aagtgaacta acagatatag aatatattgt
     3361 tacacaagaa aacggcactg aaccaccatt tatgaatgaa tattggaatc attttgctaa
     3421 aggaatttat gtagataaaa tttctggtaa acctttattt acatctgaag aaaagtttca
     3481 ttctgaatgt ggatggccta gcttttccaa agcgcttgat gacgatgaaa ttatagaatt
     3541 agtcgacaaa tcatttggta tgttgagaac tgaagtgcgt tcagaagaat caaatagtca
     3601 tttaggacat gtctttaatg atggacctaa agaaagtggc ggcttaagat actgtatcaa
     3661 ttccgctgca attcaattta ttccatatga aaagttagaa gaattgggtt atggcgattt
     3721 aatatcacat tttgataagt aggagtggaa agtatgttta aaaaattatt cggaaaaggc
     3781 aaagaagttc agaaagatat cgcaatttat gcaccattaa ctggagaatt cgtgaaaatt
     3841 gaagatattc cagatcctgt attcgcacaa aaaatgatgg gcgaaggttt tggtattaat
     3901 ccaactgaag gagaagttgt gtctccaatt gcaggacgtg ttgacaatgt ctttccaact
     3961 aagcatgcga ttgggttaaa agcagataac ggattagaat tattagttca tatcggttta
     4021 gacacagttc aattagacgg tgaaggattt gaagtgttag tgtctagtgg tgacgaagtt
     4081 aacgtcggag atccattagt aagattcaac cttgaatata ttaataataa cgctaaatct
     4141 gtaatttcac caattataat tactaacact gatcaagcag cttcaattaa tatttatgat
     4201 gaaaatgctg tgattaaagg tgaaacaaaa gtgattgatg tgacaatgaa ctaatgaaaa
     4261 actattcgtt ttatcaattt gtcatgacag ttcgtggtcg acacgacgat aaaggtcgtc
     4321 tagcagaaga gatatttgac gatcttgctt tcccaaaaca cgatgatgat tttaacatac
     4381 tgtctgatta tattgagaca catggtgatt tcacattgcc aatgtctgta tttgatgatt
     4441 tatatgaaga atatacggaa tggctaaaat tttaatataa tttttaataa taatagttag
     4501 aaccagggtg atgcaattcg ttatcctggt tttaatttaa aataaactaa gttgtgacta
     4561 aaaattaatc aattatagtg aaatatggtg cgctatcttg cataaattga tatgattaac
     4621 tacacagaat ttaaaagtac ataatacata ataaggaagt gatacaatgg atgataagca
     4681 acacacatct tcatccgatg atgaacgcgc tgaaattgca acaagcaatc aagaccaaga
     4741 aactaattca tcgaaacgcg ttcacttaaa acgttggcaa ttcatatcaa tattaattgg
     4801 tacaatccta ataaca
//
LOCUS       DAHHYJ010000331         4666 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.guided.167, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000331 DAHHYJ010000000
VERSION     DAHHYJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.guided.167"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            complement(<1..309)
                     /locus_tag="NBK22_002770"
     CDS             complement(<1..309)
                     /locus_tag="NBK22_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002475893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD2415885.1"
                     /translation="MGTLIKQECFKLFKKKSTFIIPIIIMLLMVVQAIISKNYDDVFS
                     PQSSIESAFSGFSWFIFLLIIQASTIISMEFYHGTIKNLLYRKYTRTSIIISKIITLI
                     L"
     gene            complement(311..1006)
                     /locus_tag="NBK22_002771"
     CDS             complement(311..1006)
                     /locus_tag="NBK22_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD2415886.1"
                     /translation="MDVLTVEHLTKKIGNKTILKDISLNLKRGQIVGLVGANGAGKTS
                     LMKVILGYSIYQEGNFEVIENKHKKSNVGALIENPGVYPFMSGYDNLKLLNETKNTQD
                     IDTIVSQLKMDEYIHNKAKTYSLGMKQKLGIAIAFLNQPQLIILDEPMNGLDPRAVRD
                     VRELIVKKAEEGVTFLISSHILSELVKITNSILIINKGKIVTETTEEELNQYKDNDLE
                     NVLLSIIDKEDQS"
     gene            1543..3009
                     /gene="msr(A)"
                     /locus_tag="NBK22_002772"
     CDS             1543..3009
                     /gene="msr(A)"
                     /locus_tag="NBK22_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(A)"
                     /protein_id="HCD2415887.1"
                     /translation="MEQYTIKFNQINHKLTDLRSLNIDHLYAYQFEKIALIGGNGTGK
                     TTLLNMIAQKTKPESGTVETNGEIQYFEQLNMDVENDFNTLDGSLMSELHIPMHTTDS
                     MSGGEKAKYKLANVISNYSPILLLDEPTNHLDKIGKDYLNNILKYYYGTLIIVSHDRA
                     LIDQIADTIWDIQEDGTIRVFKGNYTQYQNQYEQEQLEQQRKYEQYISEKQRLSQASK
                     AKRNQAQQMAQASSKQKNKSIAPDRLSASKQKGTVEKAAQKQAKHIEKRMEHLEEVEK
                     PQSYHEFNFPQNKIYDIHNNYPIIAQNLTLVKGSQKLLTQVRFQIPYGKNIALVGANG
                     VGKTTLLEAIYHQIEGIDCSPKVQMAYYRQLAYEDMRDVSLLQYLMDETDSSESFSRA
                     ILNNLGLNEALERSCNVLSGGERTKLSLAVLFSTKANMLILDEPTNFLDIKTLEALEM
                     FMNKYPGIILFTSHDTRFVKHVSDKKWELTGQSIHDIT"
     gene            complement(3233..3841)
                     /locus_tag="NBK22_002773"
                     /pseudo
     CDS             complement(3233..3841)
                     /locus_tag="NBK22_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3968..4546)
                     /locus_tag="NBK22_002774"
     CDS             complement(3968..4546)
                     /locus_tag="NBK22_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD2415888.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNQYGCEKIFSDHISGAKSKRPG
                     LDRAIEFARSGDTIVVWRLDRLGRNMADLITLVNELNNRGVSFHSLEENITMDKSSST
                     GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLKLLKTLYDNG
                     TPIKTIAEQWKVSRTTIYRYLNKLNNQENKDK"
BASE COUNT         1647 a          742 c          650 g         1627 t
ORIGIN      
        1 aagtattaat gtaataattt tactaataat aatgcttgtt ctcgtatatt ttcgatagag
       61 aagattttta attgtaccat gataaaactc cattgaaata atggtgctgg cttgaataat
      121 taataaaaag ataaaccatg aaaaaccact aaatgctgat tcaatagatg attgtggaga
      181 aaaaacatca tcatagtttt tagatattat agcttgaaca accattaata acataatgat
      241 aattggtata ataaacgtcg atttcttttt aaacaattta aaacattctt gcttaattaa
      301 agtgcccatt ttacgattga tcctccttat caatgatact taataataca ttttctaaat
      361 cgttatcttt atattggttt aattcttctt cggtagtttc agtaacaatt ttacctttgt
      421 taatgataag gatagaattg gtgattttta ctaactcact taatatatgg ctagaaatta
      481 aaaaggtaac gccctcttcc gcttttttta caatgagttc tctaacatct cttacagccc
      541 taggatctaa cccattcata ggttcatcta aaataattaa ttggggttga tttaagaaag
      601 ctattgcaat acctaatttt tgtttcatac ccaaggaata tgttttggct ttattatgta
      661 tatactcatc cattttaagt tgagagacaa ttgtatcaat atcttgagta tttttagttt
      721 catttagtag tttaagattg tcatagcctg acataaaggg atatactcct ggattttcga
      781 tcaatgcacc aacgttactt tttttatgct tattttcaat aacttcaaag ttaccttctt
      841 gataaatcga gtagcctaat ataactttca tcaaagatgt ttttcctgcc ccatttgctc
      901 caacaagacc gacaatctgt cctcgtttta aatttaaaga aatatctttg agaatagtct
      961 tattacctat cttttttgtt aaatgttcta ctgttaaaac atccatacaa ttatctcctt
     1021 tttaattatt tagatatatt ttaaatccaa acttaatgta tcataaaaaa ataaattatt
     1081 taaatacatg ttgagataca taaatataaa ttaaatgaag tgctgcattt tataagtaca
     1141 ctctaaatta acaagatgta taatcaatct caaattgtaa ttttatactt ttattatatg
     1201 atctttgtac ttagagatat attgtaaaaa aaccaataaa atgttatatt aacattacta
     1261 ttttatcgat gaaggaggat taaaatgaca gcttctatga gactcaaata aacctttttg
     1321 aatacaaaac taaattaatt caaaaaggct tgtccgcaat acacacactc ataggatgac
     1381 ttatgtcatt ttttatcctt cgataggaat aataaacgct ttcaaatact aaaacatcgt
     1441 aataattctt taagtgtttg aaagtgtaat gtgaatgaga cgggagtagc ctgtctcatt
     1501 ttttgcgtac acgtccttta aatatacaag gagtgtataa atatggaaca atatacaatt
     1561 aaatttaacc aaatcaatca taaattgaca gatttacgat cacttaacat cgatcatctt
     1621 tatgcttacc aatttgaaaa aatagcactt attgggggta atggtactgg caaaaccaca
     1681 ttactaaata tgattgctca aaaaacaaaa ccagaatctg gaacagttga aacgaatggc
     1741 gaaattcaat attttgaaca gcttaacatg gatgtggaaa atgattttaa cacgttagac
     1801 ggtagtttaa tgagtgaact ccatatacct atgcatacaa ccgacagtat gagtggtggt
     1861 gaaaaagcaa aatataaatt agctaatgtc atatcaaatt atagtccgat attactttta
     1921 gatgaaccta caaatcactt ggataaaatt ggtaaagatt atctgaataa tattttaaaa
     1981 tattactatg gtactttaat tatagtaagt cacgatagag cacttataga ccaaattgct
     2041 gacacaattt gggatataca agaagatggc acaataagag tgtttaaagg taattacaca
     2101 cagtatcaaa atcaatatga acaagaacag ttagaacaac aacgtaaata tgaacagtat
     2161 ataagtgaaa aacaaagatt gtcccaagcc agtaaagcta aacgaaatca agcgcaacaa
     2221 atggcacaag catcatcaaa acaaaaaaat aaaagtatag caccagatcg tttaagtgca
     2281 tcaaaacaaa aaggcacggt tgagaaggct gctcaaaaac aagctaagca tattgaaaaa
     2341 agaatggaac atttggaaga agttgaaaaa ccacaaagtt atcatgaatt caattttcca
     2401 caaaataaaa tttatgatat ccataataat tatccaatca ttgcacaaaa tctaacattg
     2461 gttaaaggaa gtcaaaaact gctaacacaa gtacgattcc aaataccata tggcaaaaat
     2521 atagcgctcg taggtgcaaa tggtgtaggt aagacaactt tacttgaagc tatttaccac
     2581 caaatagagg gaattgattg ttctcctaaa gtgcaaatgg catactatcg tcaacttgct
     2641 tatgaagaca tgcgtgacgt ttcattattg caatatttaa tggatgaaac ggattcatca
     2701 gaatcattca gtagagctat tttaaataac ttgggtttaa atgaagcact tgagcgttct
     2761 tgtaatgttt tgagtggtgg ggaaagaacg aaattatcgt tagcagtatt attttcaacg
     2821 aaagcgaata tgttaatttt ggatgaacca actaattttt tagatattaa aacattagaa
     2881 gcattagaaa tgtttatgaa taaatatcct ggaatcattt tgtttacatc acatgataca
     2941 aggtttgtta aacatgtatc agataaaaaa tgggaattaa caggacaatc tattcatgat
     3001 ataacttaaa aactatacac taatttatat tgaatatttc tttaaagtat tataataaag
     3061 taaataacaa tagtaccata accgatcatt tttgagaaaa agaatataga agaaaagaga
     3121 gggagttttt accctctctt ttcttgtttt attattaaaa tctcataata gatgattaat
     3181 atgcgtttta gaacgctgtg agaaacgctc atttgcattg acaacctatt tatcaagata
     3241 atcccttatc gtgtttaatt ctataaacag tctgccttgt tatatttact tcttttgcaa
     3301 ttttactaat tgcttggcct tcttctaaca tttcgacaac tcgatgatag ataacacgct
     3361 tttgaggatc tttcgcgttt ggcgaataaa gtaaaggtcg tcctttataa acgccttttt
     3421 cttttgcaac ttgaattcct tgtgcttgtc gacgtttact ttcgtttctc tcttgttctg
     3481 aaaccattgc taaaatttga acaattaaat ctttcataaa tttatccagt aatggattac
     3541 taataacttc attcatcata ggtaaacttg taatcattaa ctgaacgtct ttttctttta
     3601 agtaattcac agtttcaata atttcatcat aattacgtcc taaacgatca atagattcca
     3661 caacgaatcg atctcccgtt cttacaaagt taagtgcttc ttgaaatact ggtctatttt
     3721 caatagattt cccagactgt ttttctgtaa atattttttc agccccaaac gtctttaaat
     3781 tttcaagttg tctttctaaa ttttgatcga tcgatgatac tcacgcatag ccaacaatca
     3841 ttgattttca cctcataatt aatatacacc ctaaccatac attttagttg ctataaattc
     3901 aacaattata catgtatgat tagggtgtat cctgtctaat ttttatcttt cttaaaattt
     3961 agtaatatta tttatcttta ttttcttggt tatttaactt attgagataa cgatagattg
     4021 tggtacgtga aactttccat tgttctgcaa tagttttaat tggtgttcca ttatcataaa
     4081 gtgttttgag taattttaaa tcttgtttat ttaatttttc gggtcgacct ccatagcgtc
     4141 ctctagcacg agctgcaatt cgccccgctg aagaacgttc taaaattaaa ttacgttcaa
     4201 attctgcaaa tgctgcgaat aagtgaaata acaattgtcc tgtagaactt gacttatcca
     4261 ttgttatatt ttcttctaaa ctatgaaaac tcacgcctcg attattaagt tcattaacta
     4321 atgtgattaa atctgccatg ttacgtccta gtctatctag tctccaaaca acaatcgtat
     4381 ctcctgacct cgcaaattca atggctctat ctaagccagg acgtttactt tttgctccac
     4441 ttatgtgatc gctaaatatt ttttcacaac catattgatt taatcgatct tcctgcaaat
     4501 tcaaattttg caaaccagtt gaaactcttg cataacctat tttcaaaata caaacctcct
     4561 atatacacct tcagtgtacc ataacttaat gacaaggtct tttgtgaaca tagattattt
     4621 gttgggtttt tgaacacata attctatgta atacatactt atttag
//
LOCUS       DAHHYJ010000332         4120 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.guided.176, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000332 DAHHYJ010000000
VERSION     DAHHYJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.guided.176"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            6..728
                     /gene="sasD"
                     /locus_tag="NBK22_002775"
     CDS             6..728
                     /gene="sasD"
                     /locus_tag="NBK22_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070002951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasD"
                     /protein_id="HCD2415889.1"
                     /translation="MKKLATVGSLIVTSTLVFSSMPFQNAHADTTSMNVSNKQSQNVQ
                     NHRPYGGVVPQGMTQAQYTELEKALPQLSAGSNMKDYNMKLYDATQNIADKYNVIITT
                     NVGVFKPHAVRDMNGHALPLTKDGNFYQTNVDANGINHGGSEMVQNKTGHMSQQGHMN
                     QNTHMNQQPHMQQGHMQSSNHQMMSPKANMQSSNHQMNQSNKKVLPAAGESMTSSILT
                     ASIAALLLVSGLFLAFRRRSTN"
     gene            complement(922..1677)
                     /locus_tag="NBK22_002776"
     CDS             complement(922..1677)
                     /locus_tag="NBK22_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD2415890.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLARV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYNNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            1913..2620
                     /gene="deoD"
                     /locus_tag="NBK22_002777"
     CDS             1913..2620
                     /gene="deoD"
                     /locus_tag="NBK22_002777"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD2415891.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2627..3979
                     /gene="tet(38)"
                     /locus_tag="NBK22_002778"
     CDS             2627..3979
                     /gene="tet(38)"
                     /locus_tag="NBK22_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCD2415892.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFPLL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQKKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVSLNRWTQSEK
                     "
BASE COUNT         1364 a          646 c          733 g         1377 t
ORIGIN      
        1 agacaatgaa aaaattagca acagtaggtt ctttaattgt aacaagcact ttagtattct
       61 caagtatgcc ttttcaaaat gcgcatgccg acacaacttc aatgaatgtg tcgaataaac
      121 aaagccaaaa tgtacaaaat catcgtcctt atggcggagt agtaccacaa ggaatgacgc
      181 aagcacaata tactgaatta gagaaagctt taccccaatt aagcgctggc agtaatatga
      241 aagactataa tatgaaattg tatgatgcga cgcaaaatat tgctgataaa tacaatgtga
      301 taattacaac taatgtaggg gtatttaaac cacatgctgt tagagatatg aatggccatg
      361 cgttaccttt aacaaaagat ggcaattttt atcaaacgaa tgtagatgca aatggtatta
      421 atcatggtgg tagtgaaatg gtgcaaaata aaacaggtca tatgagtcaa caaggccata
      481 tgaatcagaa cacacacatg aaccaacagc cacacatgca acaaggtcat atgcaatcat
      541 caaaccatca aatgatgagt ccaaaagcaa atatgcaatc atcaaatcat caaatgaacc
      601 aaagtaacaa aaaagtttta ccagctgctg gtgaaagtat gacatcaagt atacttactg
      661 caagtattgc cgcactacta ttagtatctg gtttattctt agcatttaga cgacgttcaa
      721 caaattaata aacataatac gattaataat agaaaaatcg tgtgattatc tgagcgagcc
      781 taggacataa attaatgtcc taggctcgct aatgttatat tggcagtagt tgactgaatg
      841 aaattgcgct tgtaacaagc ttttccattt cttacccact ccctaaatag tcatcaaaaa
      901 atttcttata ttttaataat tttaataatc cgattgtctt atacgtgtca gtgttaattc
      961 agatatttcc tgtggaatat accacttatt aatcataatt ggataaggtg tttgtgcgta
     1021 caatgtttca ataatcagcc aacaatgtgt atcaccatca aacacgtgac tatgattttt
     1081 gaagtggggt gctttggtaa tagacatttt taaatctgat tgatatgcat tgttataaat
     1141 cgtttgctca acgaacgtct tcatgtcgtc ttcgttttgt gtattcactt taaatgtgtc
     1201 aatgacattt aacggtataa aggtaaagca aaatgcatca gcttgcttag aatgattgtc
     1261 ctttttttga taatagcgtt ccattgcaat gacggcagaa ggatggtttg caaacaaatg
     1321 atttgtatat tcactttcta aatcaacacg ataattaatt gatgacatag atacgcgagc
     1381 tagcaatatt tgatcaagtg gatgcttaaa ttgatccata cttgaagcgt gttgggcatt
     1441 tgtttgtgga ataacaaagt gtcccttccc tcttgtactc tctacgatgc catcttcggc
     1501 taacaatttt atagcttggc gcaaagtcat acgactgaca tcaaagcgcg cacaaagttc
     1561 cttttcagta ggtaatgcat ggccactcgg atattttcct atttgaattt ctttatataa
     1621 cgtattataa atcgttaaaa attttggttg tgtttgcgtc acgtagacaa cctccataaa
     1681 gttacttaat cactctcatc atacaataat ttttactcga attggaaaaa ttataaaaat
     1741 taaatataga taggctttga aaattagttt tatacaaggt tagtagctgt aactgtaaaa
     1801 tgttcttaat attgtcaaaa tgtaatgctt gaaagcgctt ttaaaaaata ttattatata
     1861 catggttaga caaatagaca aatcactata caaatattgg gaggaatatt ttatgaaatc
     1921 aacaccacac attaaaccaa tgaatgacgt cgaaattgca gaaacggttc tattgccagg
     1981 agatccgtta agagctaagt tcattgcaga aacttatttg gatgatgtgg aacagttcaa
     2041 tacagtgcga aacatgtttg gttttaccgg aacatataaa ggtaaaaaag tttctgtcat
     2101 gggttcaggt atgggtatgc catctattgg catttactct tatgaattaa ttcatacatt
     2161 tggttgtaaa aaattaattc gcgttggctc ttgtggcgcg atgcaagaaa acattgattt
     2221 atatgatgtg attattgcgc aaggtgcctc tactgattca aattacgttc aacaatatca
     2281 attaccaggt cattttgcgc caattgcttc ttatcaatta ttagaaaaag cagttgaaac
     2341 agcacgtgac aaaggtgtac gtcatcatgt aggtaatgtg ttatcaagtg atattttcta
     2401 taacgcggat acaacagcga gtgaacgttg gatgcgtatg ggtattttag gtgtagaaat
     2461 ggaatcagct gcattataca tgaatgcaat ttacgctggt gtcgaagcat taggtgtgtt
     2521 cacagtgagc gatcatttaa ttcatgaaac gtcaacaaca cctgaggaaa gggaacgtgc
     2581 atttacagat atgattgaaa ttgcactgtc attggtgtag atgattatga atgttgaata
     2641 ttctaaaata aagaaagcag tacctatttt attattctta tttgtattca gtttggttat
     2701 agacaactca tttaaattga tttctgtagc cattgctgat gacttaaaca tatctgtaac
     2761 gacagtaagt tggcaagcga cattagccgg tttagtaatt ggtattggcg ctgtagtata
     2821 cgcttcatta tctgatgcca ttagtatacg cacactattt atttatggcg tgatattaat
     2881 cattatcgga tcaattattg gttacatttt ccaacatcaa ttcccattac ttttagttgg
     2941 acgtattatt caaactgccg gtttagctgc tgcagagaca ttatatgtga tatatgttgc
     3001 aaagtatctt tctaaagagg accagaagac ttaccttggc ttaagtacga gcagttattc
     3061 cttgtcatta gttatcggta cattatcagg tggatttatt tctacgtatt tacactggac
     3121 aaatatgttt ttaattgcat taatcgtagt atttacgttg ccattcctat ttaaattatt
     3181 accaaaagaa aataatacga ataaagctca tttagatttt gttggcttaa ttctagtggc
     3241 aactattgct acaacagtca tgctgtttat tacgaacttt aattggttat atatgattgg
     3301 tgccttaatt gcgattatcg tttttgcgct atatattaaa aatgcgcaac gtccattagt
     3361 aaataaatca tttttccaaa aaaaacgtta tgcttcattt ttatttatag tatttgtaat
     3421 gtatgctatc caattgggtt atatttttac gttcccattc ataatggagc aaatttatca
     3481 tctgcaacta gacacaacat cactgttatt agtaccgggt tatatagtag cagtcattgt
     3541 tggtgcacta agtggtaaaa tcggcgaata tctgaattca aaacaagcga ttatcacagc
     3601 aattatttta atagcactga gcttgatttt acctgcattt gcagtaggta atcacatttc
     3661 aatcttcgtc atttctatga tattctttgc aggtagcttt gctttaatgt atgcaccttt
     3721 acttaacgaa gccattaaaa caatagatct taatatgaca ggtgtggcta ttggttttta
     3781 taatttaatt attaatgtgg cggtatctgt aggtattgcg attgctgcgg ctctaatcga
     3841 ttttaaagca ttaaatttcc caggcaatga tgcattaagt tcacatttcg gtattatttt
     3901 aattatttta ggtttaatga gtattgtcgg attagtttta ttcgtcagct taaatcgttg
     3961 gacacaatct gaaaaataaa tagatataaa ttcgcgagat atattcgtat ttatagtaaa
     4021 attaaataaa gagattatat aacacgagga gtagtaagta tgaaatttga gaaatatata
     4081 gatcacactt tattgaagcc tgagtcaaca cgtacgcaaa
//
LOCUS       DAHHYJ010000333         3852 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.guided.181, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000333 DAHHYJ010000000
VERSION     DAHHYJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.guided.181"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            <1..254
                     /locus_tag="NBK22_002779"
     CDS             <1..254
                     /locus_tag="NBK22_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCD2415893.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
     gene            complement(245..505)
                     /locus_tag="NBK22_002780"
     CDS             complement(245..505)
                     /locus_tag="NBK22_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCD2415894.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(477..1463)
                     /locus_tag="NBK22_002781"
     CDS             complement(477..1463)
                     /locus_tag="NBK22_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer MecR1"
                     /protein_id="HCD2415895.1"
                     /translation="MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1560..3569
                     /gene="mecA"
                     /locus_tag="NBK22_002782"
     CDS             1560..3569
                     /gene="mecA"
                     /locus_tag="NBK22_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCD2415896.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLGKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(3615..>3852)
                     /locus_tag="NBK22_002783"
     CDS             complement(3615..>3852)
                     /locus_tag="NBK22_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD2415897.1"
                     /translation="LWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAEITNKKS
                     IKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
BASE COUNT         1458 a          579 c          596 g         1219 t
ORIGIN      
        1 ctgtttttgg atttatgaag gctattttgg gtttcactcg gatgtctgtc cgaggactca
       61 ataaagtcaa aagagaactt ggttttgtat taatggcact taatataaga aaagtagtag
      121 ctcaacgagc tgaaaataat caaaaaattt ataaaaaaga caatttctat attatttcaa
      181 tagaaattgt cttttttcac ttatccaaga actttatgtc ccggactcat tatgatttat
      241 ttttttattg ctgacttgcc attaattctg cttgctctac aacttgttcc acagccattt
      301 tttgaagatc tggtggatag ccatatttct ttaataggcg tctaacttga actctcattt
      361 tagctttagc actgtctcgt ttagaccaat caacactcat gttatcctta actgttttag
      421 ttaactcatg tgcaatggct cttaactctt tatcgcccaa agcttcttta gctgtttcat
      481 gtgaagctcg atatactaca tacaatatac gcaacaatac gcttgtttcg attaggttta
      541 ggcattgaat cataaagtgc aatatactta acacgttctt taatatttga attaaaacct
      601 agtaaatatt gtgctgccac attatttatg tgctgagatt ttaaaataga gcattttaat
      661 atcgattcac cataacgtat atgttcatgg cgattcaaaa tttttaaaac gtttctatca
      721 catacttttt cacagtcatt gtccatcatt gttttactta tatatagtgc aggattaaac
      781 cagaatatca ttttaaaaac aacataaagc tggttgaata ttaagtcatg acttttcaca
      841 tgtgatagtt catgtagaat aatatattca atttctttgt cattcatggt ttcgactacg
      901 acagttggta gtacaatttg ggatttcact aaaccaaata ccatcggatt atcaatgttt
      961 gaactataac taattgttat atgctttttg tagaactgca tcttactttg acatacttta
     1021 agtcgttcat taagatatga cgattccaat gacgaacttt taataacatc aatttgtcgg
     1081 aatgccttaa tcatataaaa taagcacaac aaactaccaa atacccatat caaaagaatc
     1141 atatacgtta tatttgaggt ctcaaactga ttaacattaa ttgctaagtc tttcgtaaca
     1201 gatgattgtt gaccatctaa catatgacta accgaagaag tcgtgtcaga tacatttcga
     1261 ttcatcatat cttttgaaaa tgtaaaattc gatattttgt aaaatggtat taatggaatt
     1321 aacgtggaga cgagcactaa taaccaaatc ttatgtgaca taatattttg agtatatttt
     1381 atatagagca ttctcactaa aaaaattaca catatcgtga gcaatgaact gattatactt
     1441 aacattaaaa aagatgataa caccttctac acctccatat cacaaaaatt ataacattat
     1501 tttgacataa atactacatt tgtaatatac tacaaatgta gtcttatata aggagtatat
     1561 tgatgaaaaa gataaaaatt gttccactta ttttaatagt tgtagttgtc gggtttggta
     1621 tatattttta tgcttcaaaa gataaagaaa ttaataatac tattgatgca attgaagata
     1681 aaaatttcaa acaagtttat aaagatagca gttatatttc taaaagcgat aatggtgaag
     1741 tagaaatgac tgaacgtccg ataaaaatat ataatagttt aggcgttaaa gatataaaca
     1801 ttcaggatcg taaaataaaa aaagtatcta aaaataaaaa acgagtagat gctcaatata
     1861 aaattaaaac aaactacggt aacattgatc gcaacgttca atttaatttt gttaaagaag
     1921 atggtatgtg gaagttagat tgggatcata gcgtcattat tccaggaatg cagaaagacc
     1981 aaagcataca tattgaaaat ttaaaatcag aacgtggtaa aattttagac cgaaacaatg
     2041 tggaattggc caatacagga acagcatatg agataggcat cgttccaaag aatgtatcta
     2101 aaaaagatta taaagcaatc gctaaagaac taagtatttc tgaagactat atcaaacaac
     2161 aaatggatca aaattgggta caagatgata ccttcgttcc acttaaaacc gttaaaaaaa
     2221 tggatgaata tttaagtgat ttcgcaaaaa aatttcatct tacaactaat gaaacagaaa
     2281 gtcgtaacta tcctctagga aaagcgactt cacatctatt aggttatgtt ggtcccatta
     2341 actctgaaga attaaaacaa aaagaatata aaggctataa agatgatgca gttattggta
     2401 aaaagggact cgaaaaactt tacgataaaa agctccaaca tgaagatggc tatcgtgtca
     2461 caatcgttga cgataatagc aatacaatcg cacatacatt aatagagaaa aagaaaaaag
     2521 atggcaaaga tattcaacta actattgatg ctaaagttca aaagagtatt tataacaaca
     2581 tgaaaaatga ttatggctca ggtactgcta tccaccctca aacaggtgaa ttattagcac
     2641 ttgtaagcac accttcatat gacgtctatc catttatgta tggcatgagt aacgaagaat
     2701 ataataaatt aaccgaagat aaaaaagaac ctctgctcaa caagttccag attacaactt
     2761 caccaggttc aactcaaaaa atattaacag caatgattgg gttaaataac aaaacattag
     2821 acgataaaac aagttataaa atcgatggta aaggttggca aaaagataaa tcttggggtg
     2881 gttacaacgt tacaagatat gaagtggtaa atggtaatat cgacttaaaa caagcaatag
     2941 aatcatcaga taacattttc tttgctagag tagcactcga attaggcagt aagaaatttg
     3001 aaaaaggcat gaaaaaacta ggtgttggtg aagatatacc aagtgattat ccattttata
     3061 atgctcaaat ttcaaacaaa aatttagata atgaaatatt attagctgat tcaggttacg
     3121 gacaaggtga aatactgatt aacccagtac agatcctttc aatctatagc gcattagaaa
     3181 ataatggcaa tattaacgca cctcacttat taaaagacac gaaaaacaaa gtttggaaga
     3241 aaaatattat ttccaaagaa aatatcaatc tattaactga tggtatgcaa caagtcgtaa
     3301 ataaaacaca taaagaagat atttatagat cttatgcaaa cttaattggc aaatccggta
     3361 ctgcagaact caaaatgaaa caaggagaaa ctggcagaca aattgggtgg tttatatcat
     3421 atgataaaga taatccaaac atgatgatgg ctattaatgt taaagatgta caagataaag
     3481 gaatggctag ctacaatgcc aaaatctcag gtaaagtgta tgatgagcta tatgagaacg
     3541 gtaataaaaa atacgatata gatgaataac aaaacagtga agcaatccgt aacgatggtt
     3601 gcttcactgt tttattatga attattaata agtgctgtta cttctccctt aaatacaatt
     3661 tcttcatttt cattgtatgt tgaaagtgac actgtaacga gtccattttc tttttttatg
     3721 gatttcttat ttgtaatttc agcgataacg tacaatgtat tacctgggta tacaggttta
     3781 ataaatttaa cgttattcat ttgtgttcct gctacaactt cttctccgta tttaccttct
     3841 tctacccata at
//
LOCUS       DAHHYJ010000334         2336 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.guided.219, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000334 DAHHYJ010000000
VERSION     DAHHYJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.guided.219"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            70..1827
                     /gene="blaR1"
                     /locus_tag="NBK22_002784"
     CDS             70..1827
                     /gene="blaR1"
                     /locus_tag="NBK22_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD2415898.1"
                     /translation="MAKLLIMSIVSFCFIFLLLLFFRYILKRYFNYMLNYKVWYLTLL
                     AGLIPFIPIKFSLFKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAETIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLNVPFFN
                     KNILSHSFNGKKSLLKRRLINIKEANLKKQSKLILIFICIFTFLLMVIQSQFLMGQSI
                     TDYNYKKPLHNDYQILDKSKIFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPNSTYK
                     IYLAMFGLDRHIINDENSRMSWNHKHYPFDAWNKEQDLNTAMQNSVNWYFERISDQIP
                     KNYTATQLKQLNYGNKNLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGKPSGK
                     NAELISEKILKEMGVLNGQ"
     gene            1817..2197
                     /gene="blaI"
                     /locus_tag="NBK22_002785"
     CDS             1817..2197
                     /gene="blaI"
                     /locus_tag="NBK22_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCD2415899.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
BASE COUNT          948 a          266 c          334 g          788 t
ORIGIN      
        1 atcaataata ttacaattgt aatatcggtg tcaatagttt attataaata cttgaaagaa
       61 ggtgttgaaa tggctaagtt attaataatg agcatagtaa gcttttgttt tatatttcta
      121 ttgttattat tttttaggta catattaaaa cgctatttta attatatgtt aaattataaa
      181 gtttggtatc taactcttct tgcaggatta attcctttca ttcctattaa attctctctt
      241 tttaaattta ataatgtgaa taatcaagcg cccacagttg aaagtaagtc acacgacttg
      301 aaccataaca taaataccac caaacctatt caagagttca caacagatat ccataagttt
      361 aattgggatt caattgataa tatctgcaca gttatttgga tagttttagt tattatttta
      421 agttttaaat ttttgaaagc cttattatat cttaaatatt taaagaaaca gtcactttat
      481 ctaaacgaaa atgaaaaaaa taaaatagat acgatacttt tcaaccatca atataaaaaa
      541 aatattgtga ttcgaaaagc agagactatt caatctccaa taactttttg gtatgggaaa
      601 tatattattt tgattcctag ttcatatttt aaaagtgtaa ttgacaaaag actaaaatat
      661 ataatattgc atgaatatgc tcatgctaaa aatagagata ctttacattt aattatcttt
      721 aatatcttct ctattattat gagttacaat ccattagtac atattgtaaa aagaaagata
      781 attcatgaca atgaagtaga agccgataga tttgtcctta ataatattaa caaaaatgaa
      841 tttaaaactt atgcggaatc tattatggac tccgtattaa atgttccatt ttttaataaa
      901 aatatattaa gccattcatt taatggtaaa aagtcattac tcaaaagaag attaattaat
      961 ataaaagaag ccaatctgaa aaaacagtcc aaattaattc taatttttat ttgtattttt
     1021 acttttttgc tcatggtaat tcaaagccag tttttgatgg ggcaatccat aactgattat
     1081 aattataaaa agcccttaca caacgattac caaattttag ataaaagtaa aatttttggt
     1141 tcaaatagtg gatctttcgt catgtacagc atgaagaaag acaaatacta tatttataac
     1201 gaaaaagaaa gtagaaaaag gtattctcct aattcaactt ataaaattta tttagctatg
     1261 tttggacttg accgacatat tataaatgat gaaaattcac gtatgtcatg gaatcataag
     1321 cattaccctt ttgatgcttg gaataaggaa caagatttaa atacagcaat gcaaaattca
     1381 gttaattggt acttcgaacg tattagcgat caaataccaa agaactatac tgcgactcaa
     1441 ctcaagcaat taaattatgg taataaaaat ttgggaagtt ataaaagcta ttggatggaa
     1501 gatagtttga aaatatctaa tcttgaacaa gtaatagttt ttaaaaatat gatggaacaa
     1561 aataaccatt ttagtaaaaa agcaaagaat caattatctt cttcattatt gattaagaaa
     1621 aatgaaaagt atgaactgta tgggaaaaca ggtacaggta tagtaaacgg gaagtataat
     1681 aatgggtggt ttgtaggtta cgtaattaca aatcatgata agtattattt tgctacacat
     1741 ttatcagatg gaaagccatc tgggaaaaat gctgaattaa tcagtgaaaa aatattaaaa
     1801 gaaatgggtg ttttaaatgg ccaataagca agttgaaata tctatggctg aatgggatgt
     1861 tatgaatata atatggggta aaaaatcagt atcagctaat gaaattgtag ttgaaattca
     1921 aaaatataaa gaagttagcg ataaaacgat tagaacatta atcacaagac tatataaaaa
     1981 agagattata aaacgataca aatcagagaa tatttatttt tactcatcaa atattaaaga
     2041 agacgatatt aaaatgaaaa ctgctaaaac ctttcttaat aaactgtatg gaggggacat
     2101 gaaaagttta gtgctgaatt ttgcgaaaaa tgaagaatta aataacaaag aaattgaaga
     2161 attgcgagac attttaaatg atattagtaa aaagtaatta gagatatgtt tcaaacataa
     2221 tacatatgta aactatgctt gactcttatt aaattagttt tgttaataac atttaaaata
     2281 tattattaaa gatgagagtg tacagaaaat aaccattttc tgtacactcg tttttt
//
LOCUS       DAHHYJ010000335         2336 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.guided.220, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000335 DAHHYJ010000000
VERSION     DAHHYJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.guided.220"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            70..1827
                     /gene="blaR1"
                     /locus_tag="NBK22_002786"
     CDS             70..1827
                     /gene="blaR1"
                     /locus_tag="NBK22_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD2415900.1"
                     /translation="MAKLLIMSIVSFCFIFLLLLFFRYILKRYFNYMLNYKVWYLTLL
                     AGLIPFIPIKFSLFKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSI
                     DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV
                     IRKAETIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN
                     IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLNVPFFN
                     KNILSHSFNGKKSLLKRRLINIKEANLKKQSKLILIFICIFTFLLMVIQSQFLMGQSI
                     TDYNYKKPLHNDYQILDKSKIFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPNSTYK
                     IYLAMFGLDRHIINDENSRMSWNHKHYPFDAWNKEQDLNTAMQNSVNWYFERISDQIP
                     KNYTATQLKQLNYGNKNLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ
                     LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGKPSGK
                     NAELISEKILKEMGVLNGQ"
     gene            1817..2197
                     /gene="blaI"
                     /locus_tag="NBK22_002787"
     CDS             1817..2197
                     /gene="blaI"
                     /locus_tag="NBK22_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillinase repressor BlaI"
                     /protein_id="HCD2415901.1"
                     /translation="MANKQVEISMAEWDVMNIIWGKKSVSANEIVVEIQKYKEVSDKT
                     IRTLITRLYKKDIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF
                     AKNEELNNKEIEELRDILNDISKK"
BASE COUNT          948 a          266 c          333 g          789 t
ORIGIN      
        1 atcaataata ttacaattgt aatatcggtg tcaatagttt attataaata cttgaaagaa
       61 ggtgttgaaa tggctaagtt attaataatg agcatagtaa gcttttgttt tatatttcta
      121 ttgttattat tttttaggta catattaaaa cgctatttta attatatgtt aaattataaa
      181 gtttggtatc taactcttct tgcaggatta attcctttca ttcctattaa attctctctt
      241 tttaaattta ataatgtgaa taatcaagcg cccacagttg aaagtaagtc acacgacttg
      301 aaccataaca taaataccac caaacctatt caagagttca caacagatat ccataagttt
      361 aattgggatt caattgataa tatctgcaca gttatttgga tagttttagt tattatttta
      421 agttttaaat ttttgaaagc cttattatat cttaaatatt taaagaaaca gtcactttat
      481 ctaaacgaaa atgaaaaaaa taaaatagat acgatacttt tcaaccatca atataaaaaa
      541 aatattgtga ttcgaaaagc agagactatt caatctccaa taactttttg gtatgggaaa
      601 tatattattt tgattcctag ttcatatttt aaaagtgtaa ttgacaaaag actaaaatat
      661 ataatattgc atgaatatgc tcatgctaaa aatagagata ctttacattt aattatcttt
      721 aatatcttct ctattattat gagttacaat ccattagtac atattgtaaa aagaaagata
      781 attcatgaca atgaagtaga agccgataga tttgtcctta ataatattaa caaaaatgaa
      841 tttaaaactt atgcggaatc tattatggac tccgtattaa atgttccatt ttttaataaa
      901 aatatattaa gccattcatt taatggtaaa aagtcattac tcaaaagaag attaattaat
      961 ataaaagaag ccaatctgaa aaaacagtcc aaattaattc taatttttat ttgtattttt
     1021 acttttttgc tcatggtaat tcaaagccag tttttgatgg ggcaatccat aactgattat
     1081 aattataaaa agcccttaca caacgattac caaattttag ataaaagtaa aatttttggt
     1141 tcaaatagtg gatctttcgt catgtacagc atgaagaaag acaaatacta tatttataac
     1201 gaaaaagaaa gtagaaaaag gtattctcct aattcaactt ataaaattta tttagctatg
     1261 tttggacttg accgacatat tataaatgat gaaaattcac gtatgtcatg gaatcataag
     1321 cattaccctt ttgatgcttg gaataaggaa caagatttaa atacagcaat gcaaaattca
     1381 gttaattggt acttcgaacg tattagcgat caaataccaa agaactatac tgcgactcaa
     1441 ctcaagcaat taaattatgg taataaaaat ttgggaagtt ataaaagcta ttggatggaa
     1501 gatagtttga aaatatctaa tcttgaacaa gtaatagttt ttaaaaatat gatggaacaa
     1561 aataaccatt ttagtaaaaa agcaaagaat caattatctt cttcattatt gattaagaaa
     1621 aatgaaaagt atgaactgta tgggaaaaca ggtacaggta tagtaaacgg gaagtataat
     1681 aatgggtggt ttgtaggtta cgtaattaca aatcatgata agtattattt tgctacacat
     1741 ttatcagatg gaaagccatc tgggaaaaat gctgaattaa tcagtgaaaa aatattaaaa
     1801 gaaatgggtg ttttaaatgg ccaataagca agttgaaata tctatggctg aatgggatgt
     1861 tatgaatata atatggggta aaaaatcagt atcagctaat gaaattgtag ttgaaattca
     1921 aaaatataaa gaagttagcg ataaaacgat tagaacatta atcacaagac tatataaaaa
     1981 agatattata aaacgataca aatcagagaa tatttatttt tactcatcaa atattaaaga
     2041 agacgatatt aaaatgaaaa ctgctaaaac ctttcttaat aaactgtatg gaggggacat
     2101 gaaaagttta gtgctgaatt ttgcgaaaaa tgaagaatta aataacaaag aaattgaaga
     2161 attgcgagac attttaaatg atattagtaa aaagtaatta gagatatgtt tcaaacataa
     2221 tacatatgta aactatgctt gactcttatt aaattagttt tgttaataac atttaaaata
     2281 tattattaaa gatgagagtg tacagaaaat aaccattttc tgtacactcg tttttt
//
LOCUS       DAHHYJ010000336         1418 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.guided.252, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000336 DAHHYJ010000000
VERSION     DAHHYJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.guided.252"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            37..882
                     /gene="blaZ"
                     /locus_tag="NBK22_002788"
     CDS             37..882
                     /gene="blaZ"
                     /locus_tag="NBK22_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCD2415902.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLKNKSGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
BASE COUNT          595 a          167 c          206 g          450 t
ORIGIN      
        1 tataaaaatt acaactgtaa tatcggagga tttattttga aaaagttaat atttttaatt
       61 gtaattgctt tagttttaag tgcatgtaat tcaaacagtt cacatgccaa agagttaaat
      121 gatttagaaa aaaaatataa tgctcatatt ggtgtttatg ctttagatac taaaagtggt
      181 aaggaagtaa aatttaattc agataagaga tttgcctatg cttcaacttc aaaagcgata
      241 aatagtgcta ttttgttaga acaagtacct tataataagt taaataaaaa agtacatatt
      301 aacaaagatg atatagttgc ttattctcct attttagaaa aatatgtagg aaaagatatc
      361 actttaaaag cacttattga ggcttcaatg acatatagtg ataatacagc aaacaataaa
      421 attataaaag aaatcggtgg aatcaaaaaa gttaaacaac gtctaaaaga actaggagat
      481 aaagtaacaa atccagttag atatgagata gaattaaatt actattcacc aaagagcaaa
      541 aaagatactt caacacctgc tgctttcggt aagactttaa ataaacttat cgcaaatgga
      601 aaattaagca aagaaaacaa aaaattctta cttgatttaa tgttaaaaaa taaaagcgga
      661 gatactttaa ttaaagacgg tgttccaaaa gactataagg ttgctgataa aagtggtcaa
      721 gcaataacat atgcttctag aaatgatgtt gcttttgttt atcctaaggg ccaatctgaa
      781 cctattgttt tagtcatttt tacgaataaa gacaataaaa gtgataagcc aaatgataag
      841 ttgataagtg aaaccgccaa gagtgtaatg aaggaatttt aatattctaa atgcataata
      901 aatactgata acatcttata ttttgtatta tattttgtat tatcgttgac atgtataatt
      961 ttgatatcaa aaactgattt tccctctatt attttcgaga tttattttct taattctctt
     1021 taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt
     1081 ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc
     1141 atttataagg ttaaataatt ctcatatatc aagcaaagtg acaagttgtt ataaattcaa
     1201 caacttcatt tgtatgatta gggtgtacac taaacgtaca attccaataa aaagtaaaaa
     1261 tatgaagaaa agattagaag cagcgcaaat aagagaaaga aaagagagac gatcatcatt
     1321 caaaaaatat caaatagatt aggataagtc gttcgacatt ataaagtcat aaaaccaata
     1381 taaataatgc tataaaaata caagcagctt aaattagc
//
LOCUS       DAHHYJ010000337         1418 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.guided.253, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000337 DAHHYJ010000000
VERSION     DAHHYJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.guided.253"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            37..882
                     /locus_tag="NBK22_002789"
     CDS             37..882
                     /locus_tag="NBK22_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaZ family penicillin-hydrolyzing class A
                     beta-lactamase PC1"
                     /protein_id="HCD2415903.1"
                     /translation="MKKLIFLIVIALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD
                     TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKALIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKENKKFLLDLMLNNKSGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS
                     VMKEF"
BASE COUNT          594 a          167 c          206 g          451 t
ORIGIN      
        1 tataaaaatt acaactgtaa tatcggagga tttattttga aaaagttaat atttttaatt
       61 gtaattgctt tagttttaag tgcatgtaat tcaaacagtt cacatgccaa agagttaaat
      121 gatttagaaa aaaaatataa tgctcatatt ggtgtttatg ctttagatac taaaagtggt
      181 aaggaagtaa aatttaattc agataagaga tttgcctatg cttcaacttc aaaagcgata
      241 aatagtgcta ttttgttaga acaagtacct tataataagt taaataaaaa agtacatatt
      301 aacaaagatg atatagttgc ttattctcct attttagaaa aatatgtagg aaaagatatc
      361 actttaaaag cacttattga ggcttcaatg acatatagtg ataatacagc aaacaataaa
      421 attataaaag aaatcggtgg aatcaaaaaa gttaaacaac gtctaaaaga actaggagat
      481 aaagtaacaa atccagttag atatgagata gaattaaatt actattcacc aaagagcaaa
      541 aaagatactt caacacctgc tgctttcggt aagactttaa ataaacttat cgcaaatgga
      601 aaattaagca aagaaaacaa aaaattctta cttgatttaa tgttaaataa taaaagcgga
      661 gatactttaa ttaaagacgg tgttccaaaa gactataagg ttgctgataa aagtggtcaa
      721 gcaataacat atgcttctag aaatgatgtt gcttttgttt atcctaaggg ccaatctgaa
      781 cctattgttt tagtcatttt tacgaataaa gacaataaaa gtgataagcc aaatgataag
      841 ttgataagtg aaaccgccaa gagtgtaatg aaggaatttt aatattctaa atgcataata
      901 aatactgata acatcttata ttttgtatta tattttgtat tatcgttgac atgtataatt
      961 ttgatatcaa aaactgattt tccctctatt attttcgaga tttattttct taattctctt
     1021 taacaaacta gaaatattgt atatacaaaa aatcataaat aatagatgaa tagtttaatt
     1081 ataggtgttc atcaatcgaa aaagcaacgt atcttattta aagtgcgttg cttttttctc
     1141 atttataagg ttaaataatt ctcatatatc aagcaaagtg acaagttgtt ataaattcaa
     1201 caacttcatt tgtatgatta gggtgtacac taaacgtaca attccaataa aaagtaaaaa
     1261 tatgaagaaa agattagaag cagcgcaaat aagagaaaga aaagagagac gatcatcatt
     1321 caaaaaatat caaatagatt aggataagtc gttcgacatt ataaagtcat aaaaccaata
     1381 taaataatgc tataaaaata caagcagctt aaattagc
//
LOCUS       DAHHYJ010000338          547 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910025
            SAMN28744075-rid14512783.guided.310, whole genome shotgun sequence.
ACCESSION   DAHHYJ010000338 DAHHYJ010000000
VERSION     DAHHYJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744075
            Sequence Read Archive: SRR19442556
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317526.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317526.1
            Genome Coverage       :: 199x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 09:29:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,739
            Genes (coding)                    :: 2,680
            CDSs (with protein)               :: 2,680
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 59
            CDSs (without protein)            :: 59
            Pseudo Genes (ambiguous residues) :: 0 of 59
            Pseudo Genes (frameshifted)       :: 33 of 59
            Pseudo Genes (incomplete)         :: 15 of 59
            Pseudo Genes (internal stop)      :: 17 of 59
            Pseudo Genes (multiple problems)  :: 6 of 59
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744075-rid14512783.guided.310"
                     /isolate="M910025"
                     /isolation_source="Leg Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-09"
     gene            118..537
                     /gene="fosB"
                     /locus_tag="NBK22_002790"
     CDS             118..537
                     /gene="fosB"
                     /locus_tag="NBK22_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosB/FosD family fosfomycin resistance
                     bacillithiol transferase"
                     /protein_id="HCD2415904.1"
                     /translation="MLKSINHICFSVRNLNDSIHFYRDILLGKLLLTGKKTAYFELAG
                     LWIALNEEKDIPRNEIHFSYTHIAFTIDDSEFKYWHQRLKDNNVNILEGRVRDIRDRQ
                     SIYFTDPDGHKLELHTGTLEDRLNYYKEAKPHMTFYK"
BASE COUNT          214 a           73 c           88 g          172 t
ORIGIN      
        1 agttattaga gcctcttatg cagttgcgca gatcatcgta taaaaattaa tgacgtcatt
       61 tcaaaaatcg atacaaaaat aatttattat aaaaattcta agaaagaagt gaagcagatg
      121 ttaaaatcta ttaatcatat atgcttttca gtcagaaatt taaacgattc aatacatttt
      181 tatagagata ttttacttgg gaaattgctg ttgactggta aaaaaactgc ttattttgag
      241 cttgcaggcc tatggattgc tttaaatgaa gaaaaagata taccacgtaa tgaaattcac
      301 ttttcatata cacatatagc tttcactata gatgacagcg aatttaaata ttggcatcag
      361 agattaaaag ataataacgt gaatatttta gaaggaagag ttagagatat tagagataga
      421 caatcaattt actttaccga ccctgatggt cataagctag aattacatac tggcacactt
      481 gaggacagat taaattatta taaagaggct aaaccacata tgacatttta caaataaggc
      541 gtcatta
//
